LOCUS       DAVLWA010000001        87437 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLWA010000001 DAVLWA010000000
VERSION     DAVLWA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 87437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.001"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(5..76)
                     /locus_tag="VYN80_000001"
     tRNA            complement(5..76)
                     /locus_tag="VYN80_000001"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Glu,seq:ttc)
     gene            complement(170..649)
                     /locus_tag="VYN80_000002"
     CDS             complement(170..649)
                     /locus_tag="VYN80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1539989.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            complement(771..2729)
                     /gene="ftsH"
                     /locus_tag="VYN80_000003"
     CDS             complement(771..2729)
                     /gene="ftsH"
                     /locus_tag="VYN80_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1539990.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2745..3287)
                     /gene="hpt"
                     /locus_tag="VYN80_000004"
     CDS             complement(2745..3287)
                     /gene="hpt"
                     /locus_tag="VYN80_000004"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1539991.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(3291..4568)
                     /gene="tilS"
                     /locus_tag="VYN80_000005"
     CDS             complement(3291..4568)
                     /gene="tilS"
                     /locus_tag="VYN80_000005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1539992.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIINTVLYIEKIDR"
     gene            complement(4565..5833)
                     /locus_tag="VYN80_000006"
     CDS             complement(4565..5833)
                     /locus_tag="VYN80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1539993.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5953..6321)
                     /locus_tag="VYN80_000007"
     CDS             complement(5953..6321)
                     /locus_tag="VYN80_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1539994.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(6314..6580)
                     /locus_tag="VYN80_000008"
     CDS             complement(6314..6580)
                     /locus_tag="VYN80_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1539995.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6638..10147)
                     /gene="mfd"
                     /locus_tag="VYN80_000009"
     CDS             complement(6638..10147)
                     /gene="mfd"
                     /locus_tag="VYN80_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1539996.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEVEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(10148..10717)
                     /locus_tag="VYN80_000010"
     CDS             complement(10148..10717)
                     /locus_tag="VYN80_000010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1539997.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10789..11904)
                     /gene="ychF"
                     /locus_tag="VYN80_000011"
     CDS             complement(10789..11904)
                     /gene="ychF"
                     /locus_tag="VYN80_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1539998.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11988..12182)
                     /locus_tag="VYN80_000012"
     CDS             complement(11988..12182)
                     /locus_tag="VYN80_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1539999.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(12247..13383)
                     /gene="dnaN"
                     /locus_tag="VYN80_000013"
     CDS             complement(12247..13383)
                     /gene="dnaN"
                     /locus_tag="VYN80_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1540000.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(13542..14903)
                     /gene="dnaA"
                     /locus_tag="VYN80_000014"
     CDS             complement(13542..14903)
                     /gene="dnaA"
                     /locus_tag="VYN80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1540001.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(15116..15874)
                     /locus_tag="VYN80_000015"
     CDS             complement(15116..15874)
                     /locus_tag="VYN80_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1540002.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15932..17113)
                     /locus_tag="VYN80_000016"
     CDS             complement(15932..17113)
                     /locus_tag="VYN80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1540003.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            17296..17775
                     /gene="rlmH"
                     /locus_tag="VYN80_000017"
     CDS             17296..17775
                     /gene="rlmH"
                     /locus_tag="VYN80_000017"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1540004.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17819..17892
                     /locus_tag="VYN80_000018"
     tRNA            17819..17892
                     /locus_tag="VYN80_000018"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17853..17855,aa:Arg,seq:tct)
     gene            18058..18183
                     /gene="comC"
                     /locus_tag="VYN80_000019"
     CDS             18058..18183
                     /gene="comC"
                     /locus_tag="VYN80_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1540005.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            18204..19529
                     /locus_tag="VYN80_000020"
     CDS             18204..19529
                     /locus_tag="VYN80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1540006.1"
                     /translation="MDLFGFGTVIVHLLIISHNYHLICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIAIFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFS
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            19526..20278
                     /locus_tag="VYN80_000021"
     CDS             19526..20278
                     /locus_tag="VYN80_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540007.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQFIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            20330..20401
                     /locus_tag="VYN80_000022"
     tRNA            20330..20401
                     /locus_tag="VYN80_000022"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20363..20365,aa:Glu,seq:ttc)
     gene            20407..20480
                     /locus_tag="VYN80_000023"
     tRNA            20407..20480
                     /locus_tag="VYN80_000023"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20441..20443,aa:Asn,seq:gtt)
     gene            complement(20521..21063)
                     /locus_tag="VYN80_000024"
     CDS             complement(20521..21063)
                     /locus_tag="VYN80_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1540008.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            21242..22009
                     /locus_tag="VYN80_000025"
     CDS             21242..22009
                     /locus_tag="VYN80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1540009.1"
                     /translation="MIGISLIPALYNIIFLSSMWEPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQGLQGSLENLQNQSSNLST
                     LKNQANQVLPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            22013..22432
                     /locus_tag="VYN80_000026"
                     /pseudo
     CDS             22013..22432
                     /locus_tag="VYN80_000026"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22460..22774
                     /locus_tag="VYN80_000027"
     CDS             22460..22774
                     /locus_tag="VYN80_000027"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1540010.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(22875..25427)
                     /locus_tag="VYN80_000028"
     CDS             complement(22875..25427)
                     /locus_tag="VYN80_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1540011.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLNRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(25489..27111)
                     /locus_tag="VYN80_000029"
     CDS             complement(25489..27111)
                     /locus_tag="VYN80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1540012.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            27317..28342
                     /gene="trpS"
                     /locus_tag="VYN80_000030"
     CDS             27317..28342
                     /gene="trpS"
                     /locus_tag="VYN80_000030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1540013.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            28494..29972
                     /gene="guaB"
                     /locus_tag="VYN80_000031"
     CDS             28494..29972
                     /gene="guaB"
                     /locus_tag="VYN80_000031"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1540014.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(30023..31120)
                     /gene="recF"
                     /locus_tag="VYN80_000032"
     CDS             complement(30023..31120)
                     /gene="recF"
                     /locus_tag="VYN80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1540015.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(31123..31491)
                     /gene="yaaA"
                     /locus_tag="VYN80_000033"
     CDS             complement(31123..31491)
                     /gene="yaaA"
                     /locus_tag="VYN80_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1540016.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            31650..32900
                     /locus_tag="VYN80_000034"
     CDS             31650..32900
                     /locus_tag="VYN80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1540017.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32897..34180
                     /locus_tag="VYN80_000035"
     CDS             32897..34180
                     /locus_tag="VYN80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1540018.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            34222..35043
                     /locus_tag="VYN80_000036"
     CDS             34222..35043
                     /locus_tag="VYN80_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540019.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            35054..35599
                     /gene="pgsA"
                     /locus_tag="VYN80_000037"
     CDS             35054..35599
                     /gene="pgsA"
                     /locus_tag="VYN80_000037"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1540020.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            35596..36423
                     /locus_tag="VYN80_000038"
     CDS             35596..36423
                     /locus_tag="VYN80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1540021.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            36408..37247
                     /locus_tag="VYN80_000039"
     CDS             36408..37247
                     /locus_tag="VYN80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1540022.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            37240..38034
                     /locus_tag="VYN80_000040"
     CDS             37240..38034
                     /locus_tag="VYN80_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1540023.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            38093..38911
                     /gene="mreC"
                     /locus_tag="VYN80_000041"
     CDS             38093..38911
                     /gene="mreC"
                     /locus_tag="VYN80_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1540024.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38911..39405
                     /gene="mreD"
                     /locus_tag="VYN80_000042"
     CDS             38911..39405
                     /gene="mreD"
                     /locus_tag="VYN80_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1540025.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            39499..40677
                     /locus_tag="VYN80_000043"
     CDS             39499..40677
                     /locus_tag="VYN80_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1540026.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40901..41680
                     /gene="rpsB"
                     /locus_tag="VYN80_000044"
     CDS             40901..41680
                     /gene="rpsB"
                     /locus_tag="VYN80_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1540027.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            41759..42799
                     /locus_tag="VYN80_000045"
     CDS             41759..42799
                     /locus_tag="VYN80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1540028.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            43046..43369
                     /gene="tnpB"
                     /locus_tag="VYN80_000046"
     CDS             43046..43369
                     /gene="tnpB"
                     /locus_tag="VYN80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1540029.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
     gene            complement(43524..43832)
                     /locus_tag="VYN80_000047"
                     /pseudo
     CDS             complement(43524..43832)
                     /locus_tag="VYN80_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            43823..44221
                     /locus_tag="VYN80_000048"
     CDS             43823..44221
                     /locus_tag="VYN80_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1540030.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(44418..45338)
                     /gene="cysK"
                     /locus_tag="VYN80_000049"
     CDS             complement(44418..45338)
                     /gene="cysK"
                     /locus_tag="VYN80_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1540031.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(45623..46258)
                     /locus_tag="VYN80_000050"
     CDS             complement(45623..46258)
                     /locus_tag="VYN80_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1540032.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            46314..47612
                     /locus_tag="VYN80_000051"
     CDS             46314..47612
                     /locus_tag="VYN80_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1540033.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            47609..48271
                     /locus_tag="VYN80_000052"
     CDS             47609..48271
                     /locus_tag="VYN80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1540034.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            48351..48899
                     /gene="raiA"
                     /locus_tag="VYN80_000053"
     CDS             48351..48899
                     /gene="raiA"
                     /locus_tag="VYN80_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1540035.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            49035..51008
                     /locus_tag="VYN80_000054"
     CDS             49035..51008
                     /locus_tag="VYN80_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1540036.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            51005..51457
                     /gene="rplI"
                     /locus_tag="VYN80_000055"
     CDS             51005..51457
                     /gene="rplI"
                     /locus_tag="VYN80_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1540037.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            51501..52853
                     /gene="dnaB"
                     /locus_tag="VYN80_000056"
     CDS             51501..52853
                     /gene="dnaB"
                     /locus_tag="VYN80_000056"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1540038.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            52855..53121
                     /locus_tag="VYN80_000057"
     CDS             52855..53121
                     /locus_tag="VYN80_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540039.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            53210..54556
                     /gene="cbpD"
                     /locus_tag="VYN80_000058"
     CDS             53210..54556
                     /gene="cbpD"
                     /locus_tag="VYN80_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1540040.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      54639..54741
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            54812..55102
                     /locus_tag="VYN80_000059"
     CDS             54812..55102
                     /locus_tag="VYN80_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1540041.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETPH"
     gene            55065..55823
                     /locus_tag="VYN80_000060"
     CDS             55065..55823
                     /locus_tag="VYN80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540042.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            55861..56868
                     /locus_tag="VYN80_000061"
     CDS             55861..56868
                     /locus_tag="VYN80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540043.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            56868..57596
                     /locus_tag="VYN80_000062"
     CDS             56868..57596
                     /locus_tag="VYN80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540044.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            57715..58173
                     /locus_tag="VYN80_000063"
     CDS             57715..58173
                     /locus_tag="VYN80_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1540045.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            58175..60607
                     /locus_tag="VYN80_000064"
     CDS             58175..60607
                     /locus_tag="VYN80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1540046.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            60928..61581
                     /gene="rr06"
                     /locus_tag="VYN80_000065"
     CDS             60928..61581
                     /gene="rr06"
                     /locus_tag="VYN80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1540047.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            61578..62909
                     /gene="hk06"
                     /locus_tag="VYN80_000066"
     CDS             61578..62909
                     /gene="hk06"
                     /locus_tag="VYN80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1540048.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            62992..63519
                     /locus_tag="VYN80_000067"
     CDS             62992..63519
                     /locus_tag="VYN80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1540049.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            63598..65763
                     /gene="cbpA"
                     /locus_tag="VYN80_000068"
     CDS             63598..65763
                     /gene="cbpA"
                     /locus_tag="VYN80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HEV1540050.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGQDISKKYADEVKSHLEKILSEIQLDKRKHTQNLAFNKKLSRIQTEYFYLK
                     KKLKAELTSKTKEELTSKTEELTSKTKKELDAAFEQFKKDTLSTEPEKKVAEAQKKVA
                     EAKKKAEDQKEEDRRNYPTNTYKTLELEIAEFDVKVKEAELELLKEKAKESRDEEKIK
                     QAEAEVESKQAEATRLENIKTDRKKAEEEAKRKADAKLKEANVATSDQGKPKGRAKRG
                     VPGELATPDKKENDAKSSDSSVGEETLPSSSLKSGKKVAEAEKKVEEAEKKAKDQKEE
                     DRRNYPTNTYKTLELEIAESDVKVKEAELELVKEEAKEPRDEEKIKQAKAEVESKQAE
                     ATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAP
                     KPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQE
                     NGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWL
                     QNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATG
                     WLQNNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMK
                     ASQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(65993..66973)
                     /gene="dusB"
                     /locus_tag="VYN80_000069"
     CDS             complement(65993..66973)
                     /gene="dusB"
                     /locus_tag="VYN80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1540051.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(66960..67832)
                     /gene="hslO"
                     /locus_tag="VYN80_000070"
     CDS             complement(66960..67832)
                     /gene="hslO"
                     /locus_tag="VYN80_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1540052.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            67997..69424
                     /locus_tag="VYN80_000071"
     CDS             67997..69424
                     /locus_tag="VYN80_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540053.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGKDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            69583..71091
                     /gene="glpK"
                     /locus_tag="VYN80_000072"
     CDS             69583..71091
                     /gene="glpK"
                     /locus_tag="VYN80_000072"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1540054.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEIDD"
     gene            71238..73064
                     /gene="glpO"
                     /locus_tag="VYN80_000073"
     CDS             71238..73064
                     /gene="glpO"
                     /locus_tag="VYN80_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1540055.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLVDTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            73134..73838
                     /locus_tag="VYN80_000074"
     CDS             73134..73838
                     /locus_tag="VYN80_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1540056.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            74135..74338
                     /locus_tag="VYN80_000075"
     CDS             74135..74338
                     /locus_tag="VYN80_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540057.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(74425..75033)
                     /locus_tag="VYN80_000076"
     CDS             complement(74425..75033)
                     /locus_tag="VYN80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540058.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            76068..77345
                     /gene="ltrA"
                     /locus_tag="VYN80_000077"
     CDS             76068..77345
                     /gene="ltrA"
                     /locus_tag="VYN80_000077"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1540059.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            77801..78886
                     /locus_tag="VYN80_000078"
     CDS             77801..78886
                     /locus_tag="VYN80_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1540060.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYIT"
     gene            79293..79424
                     /locus_tag="VYN80_000079"
     CDS             79293..79424
                     /locus_tag="VYN80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1540061.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            79445..80995
                     /gene="dltA"
                     /locus_tag="VYN80_000080"
     CDS             79445..80995
                     /gene="dltA"
                     /locus_tag="VYN80_000080"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1540062.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVFTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            80992..82236
                     /gene="dltB"
                     /locus_tag="VYN80_000081"
     CDS             80992..82236
                     /gene="dltB"
                     /locus_tag="VYN80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1540063.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            82250..82489
                     /gene="dltC"
                     /locus_tag="VYN80_000082"
     CDS             82250..82489
                     /gene="dltC"
                     /locus_tag="VYN80_000082"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1540064.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            82482..83750
                     /gene="dltD"
                     /locus_tag="VYN80_000083"
     CDS             82482..83750
                     /gene="dltD"
                     /locus_tag="VYN80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1540065.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            83905..84345
                     /gene="adcR"
                     /locus_tag="VYN80_000084"
     CDS             83905..84345
                     /gene="adcR"
                     /locus_tag="VYN80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1540066.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            84345..85049
                     /locus_tag="VYN80_000085"
     CDS             84345..85049
                     /locus_tag="VYN80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1540067.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            85042..85848
                     /locus_tag="VYN80_000086"
     CDS             85042..85848
                     /locus_tag="VYN80_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1540068.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            85858..87363
                     /gene="adcA"
                     /locus_tag="VYN80_000087"
     CDS             85858..87363
                     /gene="adcA"
                     /locus_tag="VYN80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1540069.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT        26913 a        15419 c        17846 g        27259 t
ORIGIN      
        1 accatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
       61 ccttgaccaa cggacctgag ttgttatttt caactcttac tattatacag tcttttcaaa
      121 ctttgtcaac tactttttct aatttttttc attttttttg catgacttac taatgggtac
      181 ggatagtaaa ctccttaaac acaatgcgta agtctcttaa tactctttga cgccctcgaa
      241 atcgttcatt gcttaagacg cgttctagtt cttcttgttt ctctttactt tgtttgtttc
      301 tataatctct tagtgtttca tgaaagagat aataatcatc tagccagaga cccccctcac
      361 ttatacgatg actgatctca cccacttctt catagggttc tttatcgtat ctacgcttct
      421 gactttcctg cttacggata tagtctaaaa ttcgatttcg aaacttggtt ttgaaatatt
      481 tccttaaacg tggaatatcg tctaccagtc cttcttctct actaatcaat tcatgtaagc
      541 agagcatgcc ttcttggtcc caatccgaca attcccataa atgaaggtaa tattcatttc
      601 tacacttata cacagtccct tggacttctt catacaattc tttaatcata attttcccct
      661 ttctagatac agtctaacag attagaaaac acatttgttc cattttctcc attctgttcc
      721 ctttagctcc taaactgcac aaaaaagaga ggctccagcc tctctcaggg ttatttttcg
      781 tcattcattt ttgactttac ttcatcatag gatagtgcat gagattcctc ttctactgct
      841 tcaggcatct ttcctgtttc gtaaagagct ttaatttgtg tactatccaa tgtttcgtat
      901 ttcaataatg cttctgcaat taacttgtga gtttcacgat ttgactgaat aatttcagca
      961 gctttatttc gtgcctcatt taataatgaa cgaacctctt catcaatttc ataagctgtt
     1021 tgttctgaaa ttgatttttg aggattctgt gcaccaaaca tagcatggtt tccttcatat
     1081 tgtactgggc caagtttttc actcataccg tactctgtaa ccattgcacg tgccatttgt
     1141 gtcgcttgtt caaagtcgtt tgaagctcct gtagtttgga cattaaagat aatttcttca
     1201 gctacacgtc cacccattaa gccagccaat tgctctttca tatcttcttt agatagaagc
     1261 atttgatcct ctttaggaag tgcaatcatg tatccgcctg cacggccgcg tggtacaatt
     1321 gtaaccttat ggacaacgcg agcattcgac aagactagac caacaatggt atgtcctgcc
     1381 tcatggtaag caaccaattc tcgttctttt tgtgaaactg tcttatcttt cttagaaggt
     1441 ccagcaataa ctctatcttc tgcttcatca atatctgaag catcaattat cgatttattg
     1501 cgacgagcag caactaaagc tgcttcattc aagacattct ctaaatcagc accaacaaag
     1561 cctggagttt gttgagccac taatttcaaa tcaacatctt ctgctaaagg cttgttctta
     1621 gcgtgaactt tcaagattgc ttcacgacct ttaacatcag gacggccaac caatactttt
     1681 ctatcaaaac gtcctggacg caaaagggca ggatcaagta catctgaacg gtttgtcgca
     1741 gcgatgacga taatcccttc atttccctca aaaccatcca tctcaatcaa aagttggttc
     1801 aaggtttgtt cacgttcgtc attacctccg ccgagaccga ctccacgttg acgtccaaca
     1861 gcatcaattt catcgataaa gatgatagct ggtgctgctt ttttggcatc ctcaaaaaga
     1921 gagcgaacac gactagctcc aactccgaca aacatttcta caaagtcaga acctgagata
     1981 ctaaagaatg gaacacctgc ttctccagcg actgccttag caagcaaagt tttacctgtc
     2041 cccggaggtc cctccaaaag aacacctgct ggaatacggg ctccaagttt tgtgaatcgt
     2101 tttggatctt ttaagaactc aacaacttca actagttctt gtttttcttc ttcagctcca
     2161 gcaacatctg aaaatcttac tttaatatct tctttatttg ctgctttagc cttactacgt
     2221 ccaaaactca ttggattacg gccattgcct cctcccatat ttcccatcat agagaatagg
     2281 aagaagaata gaattccaaa tggcacaatg gatacgagta gattaatcca tataccactt
     2341 gaactttcat gcttaacagt tacttctgct ttatggtcag tagcaagttt ttgcaattct
     2401 gatacggtag tatctgcagg aagaatagtg ctggtaaatt tctctacctt agtaacagat
     2461 ggcgtgaaaa actgaatacc tgttccttct ttacttgttt taggattttt atagacacca
     2521 gaaacttcga taacactacc atttggttgg taagttaatt cttttacatt accatcggta
     2581 atttcttgta ccaactcagt atagttgatt tgctgacttc ctcctgagtt attcccagaa
     2641 tagaaatact ggaatcctgt cacaaggaaa aagataaata ataaccatag aaaaggattt
     2701 ttaattaaac cattattttg ttttttcatt aaagattatt ctttctaatt tgaatacact
     2761 tcctctttca atactccaat ataaggaaga ttacgataat tttctttgta gtctaaacca
     2821 taacctacta caaactcatt tgggatagta aagcaggtat agtctgcctc aatttctaca
     2881 acacgtcctt ctggtttatc caacaaggtt gcaattttaa cagaagctgc ttctctttct
     2941 ttaaacatat ctcgcaaatt cttcaaagtt tgacctgtat caatgatatc ttctacaaat
     3001 agaacatgtc ttcctttgat atcttgagtc acatcttgtt taatattgat aacaccacta
     3061 cttgctgttc caccatggta gctagaaacc atcatgaagt ccatttcaat atgtgtatca
     3121 atatgtttga ccaattcagc cataaaagga atagatcctt ttaaaatccc aactaagatt
     3181 ggattttttc ctgcatagtc tttagttaat tgagcaccta gttttttagc tgcttctgta
     3241 atttcatcgt gtgaaacgag gactttttta atatcgtttt ctaacatttt ttacctatct
     3301 attttttcta tataaagtac agtgttaatt atatcatttt tcgttttttt actcaaatta
     3361 ttggtcgcaa ttcccaaaat tgagacaatt tcaccaaatt gctcaataat aagagcagag
     3421 tttctctttt ccatagggat tttcaaatca ataaataaac gtctgagttt ttttctatgc
     3481 ccattttgaa tcaaaacatc tcctgttttt cgatgacgaa tgtgtatgga tgtttcacgt
     3541 gaaacaggta tttgttgaat taattcacct tctaatggaa gtccaaagga aaataaatat
     3601 ccttgataag ctacctgatt ttgatagtgt aacacaagtt catcttcctt ttcatcagcc
     3661 tgcggactga ttttacaaat ctgaaactgt tggtactctt ttatcaattc atagccattt
     3721 ttaatcggat gacgatactg gcttttagat tttaaaatct gctgaacttc agcaaactga
     3781 gcttttgtaa gattcaaatc tggaaaacga ttcagataag tttgaagtaa aactctttgt
     3841 gtagactcag agtaagaaaa taactgctgt aaatcttcca cattaatatt gttagataat
     3901 tcagctattg ccaaatcata atctaaaatt tcattgccaa tgcctaagat tgcatcccta
     3961 aatcgaggat tttctttttc caattctggt aagtaagagt ttcgaatacg atttcgaaaa
     4021 taatgattct cctgatttga tgtatcttca aagtgaaaaa ttgatggaaa gtcttttttc
     4081 tgaaaatgca agaagggacg aatgatttct atctctccga ctacttgctt ctccttaatt
     4141 cctgatagat agcgcaagcg agttcctcga atcaagcgca tcaaaatcgt ttccacctga
     4201 tcatcagcat ggtgggcagt gactaaagct gtcgcacctg tctttttcat aacctcttga
     4261 aaaaaatcat aacgaaaatt tcgtgcacgc gcttctgaaa attctcctga aaaattgctg
     4321 atataaatag gaagctctgc ttcagcagcc aacttcctta attccttttc ttcccaatct
     4381 gattcaattc tctgcttatg attcacatga gctagaatca attcaatctc taactctttt
     4441 tgataagtag acaatacctt aaatagaaac atggaatcta atccaccaga aagagctaga
     4501 accgccttag catgcttttt gaaatatccc ttcttgagaa aatgatttaa aaaatctggt
     4561 tccctcattt tagaacctca taaacatcct tggctatctt agaaatcgta tcataatcag
     4621 aattcttagt gaaaatagaa agaataaatg gagaatctgc atagacaaca cccgtatcat
     4681 gcttaaattc atccgcatct ccaattttat gagctacttt aacagaaaca cctttggcaa
     4741 ttcgctgact atcaaaatct gttttagtca aagactctag cacaaatcca ttttgattat
     4801 aaatagcttc cataaacttc ccggccatct tagaagaaat caatttttct tttggatccc
     4861 aatcatctcc cataatggca gacatcttgg atttgaatgt ggcatcagat tggtttgaaa
     4921 tgtaatatcc caatagatta tgagctacat tatcagattc ttttgatact ttcgtaatta
     4981 aatcctttaa agaatattct ttattatctt cttttttagg aagactacca cttccctctg
     5041 gtttataaga acctggaaaa tcattgactg cagatacgta ttttacagtc gtatctaact
     5101 gataaagacc ctcatttatt ttttcttgcg tataatagag ataggagagt ttcaaaacgc
     5161 tggctgcata catcttttca tcttgattga taccagcttc ttttccagta gtcagttgct
     5221 taacataaat agagaaagaa tctttctgat atttttcaga taacatttct tgaactttac
     5281 tcatccgatt atcttcttca gaagttgatt ctttagctac ccatccagcc tgatcaatat
     5341 gtagaaattc tcttccttct acaaacatgg tcttgtcgat tgatacttgc gaataagctg
     5401 ataaggatga tttcacttct tttaaatcat aaggactatt gtacagttta aagtcagatt
     5461 ctaaccatgc tttttttatt gttggagtta cctctgattg atcatataaa aatcgtttgt
     5521 ccgcagctat aaattgatga tttgatagct taaatactgg aattccttgt ttatttaagc
     5581 gccactcggt tatttgaaaa cttgttttgg gagtcaattt cccagattcc actactaaat
     5641 cttcattcgc ataaacagga acctctccat aaaccatggg agaacttaat ttttctctaa
     5701 aataaatact aaagtcagat tgtgaaaggt aataaatttc tttcgaagta tagacgactt
     5761 ctttttctgt gctaacgact tttgaaatgg tcaaaaaact tggtagcaac aaaataatta
     5821 agaatttacg cattcttcct ttccttttct tcttgtaaac gcaataattg atttttatca
     5881 gccaattctt ccagcttttc atctgacaaa ctcaaaaatt gctctattac gtctaataaa
     5941 ttttccattt tatcaccttt gaagcaagtc aggaatcgta taaacttttt ccctcgactt
     6001 agaataatag tacttcgctc gtgtatattt agcagcataa tcttcatctt tcaacttggt
     6061 agcaaatgct gtctccttat ccttttcatc actcaaagtt tgatactgag tttgcaagtc
     6121 tgctaattgc tgacgtcttt ggagtaattg ctgataactc tgcgctaaat taaaagttgg
     6181 caagataaat aatagcataa tcaaaatcaa tacccctccc ataaaacgat tccgtttttg
     6241 tcgttctttc atcaggtagc gacgacgttg gtattcattt tgaataaaag aattattcaa
     6301 ttgtacaata tttttagaca ttttcttcta cccgtgtttc actgataatt tcatacattc
     6361 ctgctgcatc ttcttttttt gtactatctt tcatctctag tacttttaca agcagcaact
     6421 tattgccaaa gcgaatttca acttggtcat taactttcaa gtccgttgaa cttttggcca
     6481 agattccatt aaccttgatt ctacctttat ctgctacttc ctttgcgact gtacgacgct
     6541 tgataattcg cgatactttt aaatatttat ctaatctcat ccttattacc tcaatttatt
     6601 attgtaccat ttttatctat tttatagaag aaaaatatca aatggaattt tcttccttaa
     6661 actcttttat ctctaataaa ctttctccaa aaatcagcag accttctaaa atttcataat
     6721 ctttcttatt ttggacatca aatacaagct ccattaatcc cttattctca gcgatgcctg
     6781 cttttaagtt cgttacggat aaagctttaa aataatcttg agctaaaaac agccgttgag
     6841 tgactttttc aaattgaatt gtaattttat tatcttttct ttccacacgt tgaacaaaga
     6901 ccttgtccaa gtatgattta accaaaccaa tctctaaaag ataggctact acatctgggt
     6961 attctccaaa acggtctatc aactcctctt gtaactcttc ataattgaca cggttgtcaa
     7021 tttgacgaat tttcttgtaa atttcaatct tatgtcgttg atcagaaata taagtatcag
     7081 gaagataggc atcaatttgc aaaatcaact cagcattccc tttggttctt gtgttagcgt
     7141 tagcattacc gtttcgttta gcaatagctt cctctaataa ctgcgaatac aattcaaaac
     7201 caacagaatc aatgaaacca gactgggatt ttcctaaaag atttcctgct ccacgaatcg
     7261 aaagatctcg cattgcaatc ttaaagccag agcccaattc tgtaaatcct ttaatcgctt
     7321 ctaatctctt ttcagagact tcactgattg atttttctgg acgatacatg agataagcat
     7381 aagcaatacg attactacga ccgactcttc ctcttaactg atataaggtt gacaagccca
     7441 tatggtccgc attttcaata aataaagtat tagcatttgg aatgtccacc cctgtctcaa
     7501 taatagtagt cgtcaccaag atatcgtatt gtccctcaat aaagtctaat agagtatttt
     7561 ccaactggac ttcactcatt cgaccatgaa catatccaat cgaagcctcc ggaatcaact
     7621 cctgtaattc tgaaaccttc tgaacaattg tgtcaacttt gttgtaaaga taataaactt
     7681 gacctccacg ctccatttca cgcaagacag catcacgaat gacactatca ttcttttcca
     7741 aaacataggt ctgaacagga tagcgattag tcggcggagt ttcaataaca gataaatctc
     7801 tgattcccag catagacata tggagggtac gagggattgg cgtagcggtc aaggttagga
     7861 catccacttg tttcttcagt tctttcaaag tttccttatg cttgacacca aatcgctgtt
     7921 cctcatcaat aatcatcaag cccaaatcag caaacacaac atcttttgac aaaacacgat
     7981 gtgttccaat caaaatatcg acttgaccgt ttttcaattt ttcaagtgtt gcagtctgct
     8041 cttttttact tctaaagcga ctcaacacat caacattaac tgcaaaattt tggaatcgtt
     8101 ccttaaaatt cgtatagtgc tgttgcgcta aaaccgtcgt cggaactaga atgacaacct
     8161 gtttgtgatc attgactgct ttaaaggctg cacgcatagc aacttcagtc tttccaaaac
     8221 caacatcccc aactaaaagt cgatccattg gctgagaagc ctgcatatcc ctcttgattt
     8281 cctcaatact acgaagttga tcatccgttt caacataagg gaaagcatca tcaaaggcat
     8341 cttgatcatc atcatcagct gagaaagcaa aacccttcaa ctgactacgt tcagagtaga
     8401 gtttgattaa atcatcagct atatcctcta cctggttctt aaccttttgc ttggcctttt
     8461 taaaatgacc gtcatttaat ttattgagtt ttggagcttt accatcactt gaaatatatt
     8521 tggacagtag atgaatctgt tccacgggga tagaaatttg atcaccattt tggtattgga
     8581 cactgacata atcgcgatga attcccttga tttcaatggt ttcaattcct agatattgac
     8641 caatcccatg gatatgatgg acaacatagt cccctttttc aagttcattg taatctttta
     8701 atctctctgc atttgaaaca tgttgtcttc gaaaacgacg ctttaatttc ttttgaaaaa
     8761 tctcatgttc agttatcaat aaaatctttt catctacaaa atgaaaacca tgtctgagat
     8821 taccctctat taagtttaca gattctttac agatacttgt cttatctcta gaatccaatt
     8881 taatctgata ttcctctaac atatcctcca atgttttact tcccattgaa ttgctagact
     8941 gcagaataat ggtgtaatcc atttttttat atcgttcaat ttcttctttt agaaaagaaa
     9001 actgattgaa aaattcctgc ataggatatt gattaaattg ataaattttg tcaaatttga
     9061 gatttcctaa acccttttga agattagaga aaaaggtcac tggactttgt tttttataga
     9121 tttgttcaat atcagaaaaa tactgcatat cagaaaatgc tttactattc tgtaattctt
     9181 ctgtaaagta ctgcgctaag tctctttcaa agacttcata ctgattcatc aatttttgat
     9241 aatcatcaaa gaatattgga gtatcttttt caatataatc aaagacagtc catgtcttat
     9301 catagcacaa agataaaaac ttccgagagt ctgcatgact ttgtttttgg tgaaaacttg
     9361 aaagaatttc ttctaggtat gatttcaaaa taggtgataa agtttttgaa atttgttttt
     9421 ctaaagctga ctgtcctcgt tgataatcct tttctctcaa aagcatatca ctagctggaa
     9481 agatagtgag ttctgtctta ttttctttcg ataattgtgt ttctacttca aatgacctga
     9541 taccatcaat ttcatcacca aaaaactcaa ttcgacaagg ttctaactgg gatatttcaa
     9601 aaatatctaa aatatctcct cgaagactaa attcgccctg agtttgtact tgagtaactt
     9661 ttcgatagcc attttccttt aactgatgga taaacgcgtg ttgatcatat tcttcaccaa
     9721 ctgagatttt tacaatacta tctttgaatg cattgggaga cggtaaaatc aatcgacttg
     9781 ctgcgatatt acaaactaaa atccctttct tagatgaatc agtcaaaaaa cgcaaggctt
     9841 caacccgtga aataattttt tcctgtgaag acatcaaaaa ctccaccata ggagcatcat
     9901 ctaccaaaaa tggatagacg agttcctcac ccaagataga aataagatca ctaacaagtc
     9961 cttctacttc tccataagtt gacgtcaata acacaatcct atcttctttt tctaaactgc
    10021 ttgcaattgc aagagcctta gtagatgttg ataaaccaag tattagttgt cttttcttat
    10081 ctgttaaatt ttgatgccat tttttaatct gatcattttc tgagaataaa tctaataagg
    10141 tcaccattta tccgttatac ctctgcattg ttttctcaaa atttttctct tgtaaatagt
    10201 attttacaga atcgtcaact ttgtcaacag actgtaaaat accgatataa tcatccctgt
    10261 caaacttact caaaacatga tgaacaactg acataccatt tttaggtctt ccaattccaa
    10321 tcttaacacg gttaaagacc tgagttccta tatgttgaat aatagacttg ataccattat
    10381 gaccacctgc tgagcctttt gctcttaaac gaattttccc aacttccatg tcaagatcat
    10441 cgtaaatgat aagtaaatcg tcaatatcca aaccatagta agttaataaa gcatgaactg
    10501 cttttccact ttcattcata aaggtcgttg gtttaaccag ataaattttt tctccattta
    10561 ggaaaaagga tgctaggtca gcttgaaata tcttatcgtg tgtaaaagtg acattctgtt
    10621 tcttcgctag ttgatcaatc aacataaaac caacattgtg ttttgtttca aaatatttat
    10681 cccctggatt tcccaagcct acaagtaatt tggtcattta tttttccttt caaaagccaa
    10741 aagggttgga attttttttc caacctaatt gacaccattt attaattttt agacattaaa
    10801 gcggaattcc atgatatcgc catcttgaac gatatattct tttccttctt cacgcaagcg
    10861 tccagcttct tttacggcct tttcagatcc gtatttcact agatcttcat atgacatggt
    10921 tactgcacga ataaagcctt tttcaaagtc tgagtggata ataccagctg cttgaggagc
    10981 cttcatacca cgtttgaaag tccaagcgcg aacttctttt tcaccagctg tgaagtaagt
    11041 tcccaatcca agcaagtggt aagctgcacg cgtcaactta tctacacctg attctgtcaa
    11101 accaatggct tcaagaaact cttttttatc ttcatcatcc aattcagaaa tttcttcctc
    11161 agcacgcgca gaaataacga ctacttcagc attttctgtc gctgcaaatt cacgaatttg
    11221 tttgacatag tcgatagagt caggttctga aaccacatcc tcgtccacat tagctacata
    11281 aagaactggt ttagtcgtca aaaggaaaag acctttgaca accttttgtt cctcatctgt
    11341 aaattcaatg gtacgagctg atttcccgtc ttctaggact ggtttaatct tttgaagaac
    11401 attgaattct gctactgatt ctttatcttt ttgcgtacgt gccatctttt ctacacgcgc
    11461 atatcgtttg ttcactgatt ctaagtcagc aagaatcaat tccagattaa ttgtatcaat
    11521 atctgcaagt ggatctacaa aggcgtcttc acgtccttgc tcgcgcatca cattttcatc
    11581 atcaaaagca cgaactacgt gaacaatcgc atctacttca cgaatattgg ccaagaattt
    11641 attccctagc ccttctcctt ttgaagctcc ttttacaatc cctgcaatat ctgtaaattc
    11701 aaatgttgtg ggaactgtct ttttaggagt tatcatttca gttagttttt gtaggcgttc
    11761 atctggaact tccaccattc caacatttgg atcaatagtc gcaaatgggt agtttgctgc
    11821 ctctgctcct gcttttgtaa ttgcattaaa taatgttgat ttaccaacgt ttggcaaacc
    11881 aacgatacct gctgtcaaag ccatatttct cattctccgt tttcatttca atccctaata
    11941 ttataacaca aaaagggata aagtgctaac cctctaacta agggttctca gtcaataatt
    12001 ttattcattt ttcgctcaaa atcatatcgc cccatcatga caacatgctc acaattactg
    12061 cattttattt tgatatctgc tcctacacgt gtaatttccc aacgattagc ctttttacca
    12121 gtcgacttga ttgtacaagc gtgtgatttt ttcatctcaa caaaatttcc aacttgatac
    12181 atactactct ccttttcttt ttcgattata tcataaaaga ggcgagccag gctcaacctc
    12241 tttcacttaa tttgtacgaa ctggtgtaat gagctgcatg aagtcttcgt cagtatctgc
    12301 tggcacaaga gtaaatggac gaacagctga gataaagcta atggtcacct tttcgctatt
    12361 taaagcttta agagaatcaa tcaagtaagt tgggttgaaa ctaatggtca aatcttcacc
    12421 agtaacctga tcagtatcga tttcttcgtt tactttacca acttctggag agtgaacatg
    12481 ggcgctaaca accccatcct taatttcaag tttcacagta ccattttgag tcgcacttga
    12541 taaaagacgg gcacgctcca ttgactggcg taagtttacc acattaaaag taatagtagt
    12601 gttaaagtct gttggaatca agcgatctgt atcaggatag tttccttcta ggagacgagt
    12661 atagaagcta atattttcgc ttctaaagag gatttggtta ttggcaaaga aaatctctac
    12721 agtttcgata tcatctgtaa ataccgctga aaattcgcgt agagaacggc taggaattac
    12781 gacatcaaaa tcatcactat ttttttcaag agtcaatttt ttctggctta ggcgatgaga
    12841 gtctgttgca actgttttta actctttgtg ttgactcaat acgaagtgga cacctgttaa
    12901 aatcggacga ctctcttgtg tacttgcagc aaaggctgtt tcattaataa ttttcttgag
    12961 taattttgtt tcaagtatta aaggagtgct tgctgaaatt tcttggattc gtggatattg
    13021 ttcgctatct tttcctttta gggtaatttc tgatttgcca ctggttaaaa caatttgatt
    13081 ttgttcaatt tctttaaaat caagagtcac atcaggtaaa ctagatacta cattgataaa
    13141 gaaagaagct tcaagaagga tcgaacctaa agaagtaatt aacaaaccag catcttcatt
    13201 tttttgagaa ataaaatttt caattgaaat ttgaccattt gaaccaatta aagtaatacc
    13261 ttcattggtc acgtcaattt ttactgttga taaaatagga atggcatttt tagaactaat
    13321 agctctctta gtagtattta atgcttgtag aaataaattt ttattaattg aaaaatgaat
    13381 catggattct cctttattta tttttagtat tagaaagata atagtaataa tagagtctgt
    13441 gaaatctgtg gaaaacagcc tttaaacaag tcatatcaag ttttttagct tgtttaaaaa
    13501 atgtggataa aaaagataaa aaactaaaag ttatccacaa attatttgat tttctttttg
    13561 attgattcaa tttctaaacg taaattatcg tcttgatcaa tcaaagattt tattttggca
    13621 tgggcatgaa tgactgtggt atgatctttt cccccaaatt ccttcccaat ttttggaaga
    13681 ctattatctg ttagttctct agataaatac atggctactt gacgggccaa aacaatattt
    13741 tgaaggcgtc tacttccctt catttctttg atactaacac cataaaagtt accaacttca
    13801 gtttggattt tatcaattgg gatgacgagc atttggctaa catcttgttt gcgggctcta
    13861 atggcttctg cagcaatatc aatagtgata tccttgattt tttttactct ggcaattaaa
    13921 gtgatgtcgt tgatggctcc ctcaagatct cgaacatttg aatcaaattg cccagctagg
    13981 tattctagag tatcactttg gaaattgtag cctaaatgtt ccgttttact ttgtaaaatg
    14041 gcaatacgtg tttcaaagtc agggggtgtg atagtttgtg tcaatcccca actaaaacgc
    14101 gtgacaagcc tctcctcgag cccttctaga tgttttggac tacgatcact cgttaggaca
    14161 atctgttttt gcttgtcatg aagggcgtta aaggtattga aaaattcttc ctgagttgcg
    14221 actttttttc cgctgagtga ctgtatatca tcgattaaca aaagatcaag actacgatag
    14281 gtctttttaa acttttccat ttccccaagt cttaggtgat caagaaagtc attaataaag
    14341 ctttcggcag ggatatattt aacacgcgca ttaggaatat tttttagaat ttcatttcca
    14401 atagcgttta ataagtgagt cttaccaagg cctggtcctc catagataaa aagagggtta
    14461 taggtcagag ccaaatcttc agagacagct aaagcggctg atacagccca aacatttcca
    14521 tccccttgaa taaagttatc aaaggtatac ttttctttta atcccgtatc tgaataagga
    14581 atagatacta actttggact atagtcataa agagttaaat ttgtagcttc ttcaacttgt
    14641 gagctagtcg tatcttgagg tttggtgaaa atatagtggg gagttatttc agcgtcataa
    14701 atttcaaaac cagctactac aataatatct tttagttgtt tttcccagac catttccatt
    14761 tcagagcgag gtagaaatat agtggcaaca ttttcctcta ccttgatgag ttcagcttga
    14821 atagcataga aatcatacat ggatcgagtc agtctttctt gtgcaaattc taatatacga
    14881 ttccaaaatt gtttttcttt caatcctttc tcctccttta ctatttaaaa gtttaatact
    14941 agagatattt taccatagat agcaagaatt ttccacaagc tgtgaaaaat aatccacaat
    15001 gttactaaac tttatccaca ggttggggat aaaagaagaa attattgatt tattaagctt
    15061 tttatcgaaa aacatagtgg ataaccttgt gagataaaaa aataaaagaa cagccttatt
    15121 tcaggctgtt gataattcta ctatattctt cttgatttga aaaagaaata atgatttttc
    15181 cactgtcttt tttagatagt ttaatttcta catctaatcc gagtagtttt cttaactgtt
    15241 tttcttcatt ttgtatgaaa tgattagttt tttgctgttt cttttgtttt ttctctgtca
    15301 gaagagcttc taacttcctt acagaaatat cttcttctat aatccgttga aagaaatagt
    15361 cttgttgttc cttatttaac ccaactaggg aacgcgcatg ggcttgtgat agtttgccat
    15421 tttctacttc tgagagaatc tgttctggca aggaaagtaa acgaatggag ttgctgatat
    15481 atggacgaga cttgcccatc ttatctgcaa tttcagcatg ggtgaatcct ttctctacga
    15541 gagattcata ggcgcgtgct tcttctattg ggtttaaatt ttctctctgt aaattttcaa
    15601 taatggactg gaccatcatc tcttggtctg aaatctgttt aacaacagct gggatagacc
    15661 gtagaccagc taaaagtgaa gcccgatagc gtctctctcc tgcaaggatt tcataaccaa
    15721 taacaggaga ttgacgaaca ataatcggtt gaatgacccc attttctttg atagactgtg
    15781 ctagttcatc tagtttttct ctatcaaatt cttttcgggg ttgataggga tttttttgta
    15841 tatctgtgat agaaatcatt tcaaattttt ccatgattct acactaacac atcttttctc
    15901 ttatgtaaag ctttctttac atagatgtca attaagattc taaatcacct gaactcttgt
    15961 taagtttgat agaggtagtt tcttctttcc cgttacgata gtaggttatc ttaatggtgt
    16021 ctccgataga atggttgtaa agagcacttt gtaaatctgt tgatgaagca atctctttgt
    16081 catctacttt tgtaattaca tcgtattttt caaggtgacc attggcaggc atattacttt
    16141 gtaccgaacg aacaactaca ccagatgtaa cattacttgg aatattgagt cttctgatgt
    16201 cgcttgtact cacattagat aaattaacca tctggattcc caaagctgga cgagtcactt
    16261 ttccgttttt ttctaactgt tcaataatat tgatagcatc atttgcagga attgcgaaac
    16321 caagaccttc tacagatgtt cctccatttg tagcaatttt acttgaggta attccgataa
    16381 cctgcccttg aatattgatc agtgggccgc cagagttacc tgggttaata gcagtatcag
    16441 tttggatggc ttttgtagaa atagcttgtc catcttccga tcttaaggat acatttctat
    16501 tgagactgga tacgatacct tgagtgacag tatttgcata ttcagaacct aacgggctac
    16561 cgatggcaat agcagtttct cctacagtta acttactaga atcaccaaac tcagctactg
    16621 ttgtcacttt ttctgaagag attttgacga cagcaatatc agagaaagtg tcagctccga
    16681 caatttctcc aggtacttta gtcccatctg acaatcgaat atctactttg ctggcgccat
    16741 ttataacgtg attgttggtg acgatgtaag cttctttatc attcttttta taaataactc
    16801 cagatccttc actagagatt cgctgagaat ctgtgtcagt atcatcattg ccaaatacgc
    16861 tattttgtct gtttgccgaa taagtaataa cagaaacaac agcatctttt actttgttaa
    16921 cagcctgtgt tgttgaattt tcgttcttat aggcagtttg tgtaatagta ctattgttgt
    16981 tagagttgtt tacactactt ttttgagtta gttgagttat tgaaaaacta cccaaggctc
    17041 cactaaaaaa gctaatgacg ataacgacta ataattgaaa ccattttttg taaaatgttt
    17101 ttagatgttt catatttgcc tccatatgtt tgaattactg aaagtataaa ctgactagct
    17161 taattataac ttaaacacaa aagttttaca caaactgtgg ataactcttt tgaaactgtg
    17221 attttcttaa ttgaaatcta ttttttattt tgtgaataag atgtgaaaaa atagagaata
    17281 tgttagaata gagtcatgaa aattaaagtt gtaacagttg ggaaactgaa agaaaagtat
    17341 ttaaaagatg gtatcgcaga gtattcaaaa cgaatttcta gatttgctaa gtttgaaatg
    17401 attgagttat cagatgaaaa aacaccagat aaggccagtg aatcagaaaa tcaaaagatt
    17461 ttagaaatag aaggtcagag aattttatca aaaattgctg accgtgattt cgttattgtg
    17521 ttagccattg aagggaaaac tttcttctca gaagaattta gtaagcagtt agaagaaact
    17581 tctataaaag gattttctac tcttactttt attattgggg gaagtttagg attgtcatca
    17641 tctgtaaaaa atagagccaa tctttctgtc agttttggtc gcctaacctt acctcatcag
    17701 ttaatgagac tagttcttgt tgaacaaatc tatcgcgctt ttacgattca gcagggattc
    17761 ccctaccata aatagaaaat tgacttttaa ttgaattttt ggtagaataa ttgtgttagg
    17821 tctcatagct cagctggata gagcattcgc cttctaagcg aacggtcgca ggttcgaatc
    17881 ctgctgggat caatataata gcaaagctgg gaattttccc ggcttttttc ttaaaaaagt
    17941 acactttggg agaaaaaaat gacagttgag agaattttat ctaaaacgaa attccatttt
    18001 gtataatggt ttttgtaagt tagcttacaa gaaaaaaaca ttttaggaga ttttattatg
    18061 aaaaacacag ttaaattgga acagtttgta gctttgaagg aaaaagactt acaaaagatt
    18121 aaaggtgggg agatgaggct gtcaaaattc ttccgtgatt ttattttaca aagaaaaaag
    18181 taatgaaata aggggaaaga gtaatggatt tatttggatt tgggacagtt attgttcatc
    18241 ttttaattat tagtcacaac taccatttaa tttgtaaagg tcaaataaat agaaaagaat
    18301 tattcgtttt tggtgcttat acattactaa cagaaatagt atttgatttt cccttatata
    18361 ttctatattt agatgggtta gggattgcaa tatttttatt tcctttgggc ttatattcct
    18421 attttcgatg gatgaaacag tatgagaggg atagaggact attcctaagt ttactactat
    18481 ctcttttata tgagagcact cataactttc tgtccgtaac tttctcctct ataacaggag
    18541 ataattttgt tttacaatat catttcccat tctttttcgt tgtgacggtg ttaacctatt
    18601 ttgttacatt aaaaatcatt tattatttcc atttggaact agcctatttt gacaaggact
    18661 acctttatcc tttcttgaaa aaagtatttt ttgctttact attgctacat attgtatctt
    18721 tcgtttcaga tatggtaagt acgattaaac atttgaatag ttttggaagt attttgtcat
    18781 ctattgtctt tatctctctc cttttgacct tctttgcaat gaattctcat aaagttcaaa
    18841 tggagaaaga gattgctttg aagcagaaga aatttgaaca gaaacattta cagaattaca
    18901 cagatgaaat tgtcggtctg tataatgaaa tccgtggttt tcgacatgat tatgctggga
    18961 tgcttgtcag catgcagatg gcaattgaca gtggtaattt acaggaaatt gacagaattt
    19021 acaatgaagt tttagtcaaa gcaaatcata aattgcgttc agataagtac acttactttg
    19081 atttgaacaa catagaagac tcagctttac gaagtttggt tgctcagtca attgtctatg
    19141 ctcgaaataa tggtgtagag tttacactgg aagtaaaaga tacgattacc aagcttccaa
    19201 ttgaactatt ggatttggtt cgtatcatga gcgttttatt gaataatgct gtcgaaggat
    19261 cggctgatag ctataaaaag cagatggaag tagcagttat taagatggaa actgaaacag
    19321 ttattgtgat tcagaattca tgtaaaatga cgatgactcc ttcaggagat ctattttcct
    19381 taggattctc cactaaggga agaaatcgcg gagtcggatt aaataatgtg aaagaactac
    19441 tagataagta caacaatatt attttagaaa cagagatgga aggcagtaca tttagacaaa
    19501 tcattagatt taagagggaa tttgaatgaa agttttaatt ttagaagatg ttattgaaca
    19561 tcaagtgaga ctagagagaa tattggatga aatttcgaaa gaatcgaata ttccaatatc
    19621 atacaagaca acgggaaaag tccgtgaatt tgaagaatac attgaaaatg atgaagtaaa
    19681 tcagctttat ttcctagata tcgatattca tggaattgag aaaaagggat ttgaagtggc
    19741 tcagttcatt cgtcattaca atccttacgc tattatcgtc tttatcacta gtcgatcaga
    19801 gtttgcgact ctaacctata aataccaggt atcagcccta gattttgttg ataaggatat
    19861 caatgatgag atgtttaaga agagaattga gcaaaatatc ttctacacga agagtatgtt
    19921 acttgaaaat gaagatgttg tagattattt cgactacaat tacaagggaa atgatttaaa
    19981 aattccttac catgatattt tgtatattga aacaacaggg gtatctcata aattgcgcat
    20041 tattggtaag aattttgcaa aagagtttta tggtaccatg acagatattc aggaaaagga
    20101 caaacatact cagcgatttt attctcctca caagtcattt ttggtaaata taggcaatat
    20161 cagagaaatt gatcgaaaaa acttagaaat tgttttctat gaagaccatc gttgtcctat
    20221 ttcaagatta aaaattagaa aattaaaaga tattttagag aaaaaatctc aaaagtgatt
    20281 gacaattagc aagaaattga tataatggtt atatctgcta cagatagaag gtccgttggt
    20341 caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc cgtacggact
    20401 attttatccg ccatagctca gttggtagta gcgcatgact gttaatcatg atgtcgtagg
    20461 ttcgagtcct actggcggag tatagataaa agggaacaca gctgtgttcc tctttttgta
    20521 tcaatttgta tcaccaagca ttttcataag gaagtctgtt atttcttgag aactttcttt
    20581 ttttccatgt gcaatccaag tttggcagac accaaaaagt gcatgagtta gatagatgct
    20641 actatattct aattcagtgg tatttagatt cagttgcata aatcgctttt gtaaatctgt
    20701 actgagcatg atatgaagtt tatttcgtaa gaaattttgg atttctttag tcccattttc
    20761 agaaagaagg gcagccagaa gtggttctga ctctagatat tcaaaaactt ctaaaatagc
    20821 gtctcttttg tgatgagcat gtttttgaaa aatatattca aatgtatgga atagcttgct
    20881 ttgatagtgc tcaatcatat catacttatc cttatagtga gtatagaagc tggaacgact
    20941 aattccggct ttttctacta atttgacagt agaaatttta tcaaatggct gttccatcag
    21001 taattgtacc atagcgtttt caatagttcg ctttgttttt aagcgtttgt tactttcttg
    21061 catatttcct ccttgtaaac aaattagact atatgtctaa aaatagattt tttatcttgt
    21121 aatttagatt ttttaatgta taatctatta tatcaaaatt ttagacaata tgtttaaaaa
    21181 aggagaaact atgtttaaag aatggaaagc aatttaaaaa aaaacaacct ttattattgt
    21241 catgatcggg atttctctta ttccagctct gtacaatatc atatttttgt catcaatgtg
    21301 ggagccatat gggcaattgt ctgacttacc tgtggcagtt gtaaataatg ataaagaggc
    21361 ttcctataat ggtaatacta tggcaatagg aaaagacatg gtgtccaatt taaaagaaaa
    21421 taaaaccttg gattttcatt ttgtagatga agaggaagga aagaagggat tggaagatgg
    21481 cgattactat atggtagtga ctttaccaag tgatttatct gaaaaaacaa ctacattatc
    21541 caatattcaa tcgacagcag cttatcaatc attgacaagt gagcaacaaa ctgagataag
    21601 tgattctgta tctcaaaatt caactgatag tattcaatcg gctcagtcaa ttgtagcttt
    21661 agtacaaggt ttacagggaa gtttagaaaa cttacaaaat caatcttcta atctttcgac
    21721 tttaaaaaat caagctaatc aagtattacc tattacttct acttctttga taggattgtc
    21781 aagtggatta acagagatac aaggagatgt tactagcaaa ttagttcctg ccagtcagtc
    21841 gattgcatca ggtgtaaacg catatactac aggtgttgat aaagtttctc agggcgcaag
    21901 tcaactaagt gaaaaaaatg ccaccttgac aggtagtttg gataaactag tttcaggctc
    21961 aaacaccttg acacaaaaat cttctagatt gacagcagga gttggttaat tacaatcagg
    22021 atctgggcaa ttagcagaca aatccagtca gttactttca ggtgcttctc cattagagaa
    22081 tagagctaat aaattggcag atggatctag gaaactagca gaaggtggaa caaagttaac
    22141 ttctggattg gaagatttac agacaggact tgcttcttta ggacaaggac taggtaatgc
    22201 tagtgatcaa ctcaaatcag tatcaacaga atctaaaaat gcagagattt tgtcaaatcc
    22261 actcaatctt tcaaaaacag acaatgatca agttcctgta aatggaatcg caatagctcc
    22321 ttatatgata tcagttgctc ttttttttgc agcaatatca acaaatatga tatttgcgaa
    22381 attgccttca ggacgtcatc cagagagccg ttgggcttgg ttgaaatctt gagctgaaat
    22441 aaatggtatt atagctgttt tggcaggaat tttggtatat ggaggagttc agcttattgg
    22501 tttaactgct aatcatgaga tgagaatatt tattctcatc atcctaacaa gtttagtatt
    22561 catgtctatg gtgaccactt tagcaacgtg gaatagccgt ataggagctt ttttctcact
    22621 tattttgctt ttactacagt tagcatcaag tgcaggtact tatccacttg ctttgacaaa
    22681 tgatttcttt agatctatta atccctggtt accaatgagc tattcagttt cgggattacg
    22741 acaaacaatc tctatcaaca agtcattttc ctagctgtca tactagctct atttactggt
    22801 ttaggtatgc tagcctatca acataagaaa atggaagaag attaaaaaaa tcgaccgatt
    22861 aactggtcga ttttttatgc cttagatgac tttcgtctgt gattatagat tccaaatagt
    22921 aagagagaag taaaggaaca gattgctcca gtaataaaac cattgggaat gaaggaaagt
    22981 gtaatagttc ctttcccctt gggaatgtca actttcataa atccagtttg agcttgttta
    23041 atttctattt tcttaccatc ttggtaggca gaccaacctt tgtcataagg aatggtgaag
    23101 aaaatagatg tatcttgttg gacatcatat gtagcaaaaa ccttgttttt agaagttgat
    23161 actgtgacag gttgttcttt aattttttga attgcctcgg tgaaagtttt ggtatctaaa
    23221 cgatagaagg taggagattc aaatgatact tgtgaatttc cagggaaact aacattgata
    23281 ttgaaagttt ttttctcttt agtatatcct agattaaaga aggagaagac attatcagtt
    23341 gtaaaagtct ttttttcacc atttacaagg atgtcaacct tcttttgttt atcgttagaa
    23401 aagtgaaggt ttatgaaaga gagataaact tggctgtttt ctggaacttc aatttgatac
    23461 tggattgctg catcttcatt tgaagaactt gtgacactaa tcaaatcatt agtattttct
    23521 attttttctg ttttttcata aggtattgga gaaaaataat caaaattgac gttagcaagt
    23581 tgatttaaaa atgaggcctg attatccaag gtatgttcat tgaacttgac atcattgtaa
    23641 acagattgac tagcaactgc aatcggaaga gagtattgat tttcatatag ggtaagatta
    23701 tctttttgat agatatcttt aaagccatac ttatcaatag gactgtctga gatattgtac
    23761 tggataccaa ataaactatc agccaaaata ctattatttg catatcggag attgagatta
    23821 gtcccagagg atttaaaacc aagtttatct aaagtagagc ttgatgaacg atttcgaaca
    23881 gatgaaaatt gagagattcc attgtagttg aatttcatac tgtcatttcc tgtctgagtt
    23941 tgtagttttt cagtacgagt aaattgattt ccaatatatg ttgagaaaga ttccatagct
    24001 gggatatctc gactataagc acttcgagaa gcaaatcccc attccttagc aattccgtcc
    24061 atttgagatg aagcatttaa actcatttca accagtataa ataaagagat tagaatggca
    24121 aatagattca cagatataaa ctttttgata actgcaagga gtaaaagaga atagacaacc
    24181 aaaaattcaa gagtaagcag aatattcaaa tctgttaaaa aagaataatg cgattttaga
    24241 tagatggtag ctaaaaatcc tgctactaca agaaaaagcg aaactaaaaa attccagact
    24301 ttaagttctt tcagacgatt taagacttct gctgctgtgt aaattaacaa ggtagagaaa
    24361 atccaagcat agcgatgtaa aaacatgttt ggagtatgca tgccttgcca aaataagtca
    24421 agagcttcta tgtaaaagct tgcaattaga aatgcaaaga atattacata tatgagtttc
    24481 acgtgaaact taatagattt cagcgtaaaa aataaaatgg tcaaaataaa gggaagtagt
    24541 ccaacaaaaa tcattgggat ggccccatac tttgttgtgt caaaggaacc aatgaattgc
    24601 ttagcaaaga gatcaagata ccagctactt tcagtttgaa actttgtaac ttcagtcaat
    24661 ttttccccat gtgtctgtaa atcaaacaga gtgggaagag tcataatcaa actagccata
    24721 ccagctaaaa aggagataac tatgaaatca agaacagatg attttcgagt cttaaagtcc
    24781 cacgaaattt gacagagata ccagaaaata agaaacaata ctgtcatata tccaaaataa
    24841 taattttgaa taaataagat tgacagactt gtaaagtaca ataggagttt cttttcagtt
    24901 atcagtagat gtaaaccagt tataattaaa ggaatcaaga taaaaacatc tagccaggtt
    24961 tttatctcta attgactgac agtgaaactc atcagagcat aggaagtaga taaggctagt
    25021 tttaaaatct gagggataga ttgaaacaat ttattcaaac taaaaaaggt tgacagacca
    25081 atcaatccaa attttaagag agttgtcaga tagatagcat ctggcatatt cgttagatca
    25141 aaaaagtaaa ccagaggcgc gagaaaacta cccaagtaat aactagatag ggcatagaag
    25201 tttagcccta gaccacttgt aaaggtgtaa aacagattac tatttccatg taggatattt
    25261 cgtaaggcta catcaaaaat aacgtattga tgaaagccat ctcctaatag aggagagttg
    25321 tcgctattcc agtagatacc ttgagataga tatactccag acataatcac tacaggaatg
    25381 atgaaagaaa taaaataggt tcgatatgtt tttaaaaatg atttcatgtt acctcgtaga
    25441 atgatagaaa actcagttgg ttaacccaac tgagttttga agttttattt agtctttcca
    25501 aagttcttta acttttgctt gtacttctgc attttctagg aattcatcgt aggtttcatc
    25561 gatacggtca atgacgccat ttttagataa gacaatgata tggttagcca aagtttgaat
    25621 aaattcgtgg tcatggctgg caaagatgat tgattcttta aagtttttca atccatcatt
    25681 caagcttgag atagattcca agtccaagtg atttgttgga tcatcaagta caaggacatt
    25741 tgattttaag agcatgagtt ttgaaagcat gacacgaact ttttctcccc ctgacaagac
    25801 atttacaggt ttgttaactt catctccaga gaagagcata cggccgagga agccacgtag
    25861 gaaagtattg tcatcttctt ctttacttgc gaattgacgc aaccagtcaa gaattgattc
    25921 tcctcctgca aaatcagctg agttatcttt tggtaggtaa gattgactag ttgtaactcc
    25981 ccacttgaca gttccttcat agtcaatatc tcccatgatt gcacgaatta atgcagtcgt
    26041 ttgaatatca ttttgtccaa taagtgctgt cttatcatct ggacgcaaga tgaaactaat
    26101 attatccaag atagtttcac catcaatctt tacagttaaa ttttctactg tcaagagatc
    26161 attaccaatc tcacgttccg ctttaaagtt gataaatgga tatttacgac tagatggcac
    26221 aatctcttct agctcaatct tatcaagcat tttcttacgt gatgttgcct gccttgactt
    26281 agaagcattg gcagagaaac gagcaacaaa ttcttgcaat tgtttaattt tttcttctgc
    26341 tttagcatta cggtctgcta gcaatttagc agcaagctca gaagattcct tccagaagtc
    26401 gtagtttccg acatagagtt tgatttttcc aaagtcaagg tcggccatgt gagtacaaac
    26461 tttgtttaag aagtgacggt cgtgggatac tacgataact gtgttatcaa agtcaatcaa
    26521 gaagtcttct aaccaagtaa tcgattggat atccaaaccg ttagtaggct cgtccaagag
    26581 aagaacatct ggtttaccaa aaagtgcttt ggcgaggaga acctttactt tttcaccgtt
    26641 ggccaattcg ctcatgtttt ggtagtgtaa ttcttctgga atgtttaggt tttgaagtag
    26701 ttgagaggct tcactctctg cttcccaacc tccaagttcg gcaaactctc cttcgagttc
    26761 ggcagcacga accccgtcct cgtctgagaa atcttccttc atgtagatag catctttctc
    26821 tttcatgatg ctataaagtt tttcatttcc catgataacg acatcaatgg cacgttcatc
    26881 ttcgtagtca aagtgatttt gacgaagaac agagagacgt tcatctggac caagagagat
    26941 gtgaccagta gtaggttcga tatctccagc taaaattttt aaaaaggttg attttccggc
    27001 accattagca ccgattaatc cgtaagtatt tccttctgta aatttgatat tgacatcatc
    27061 aaaaagtttg cgatcactaa aacgtagtga aacatcagat actgtaagca atgtttttct
    27121 cctatatgtg taatatattt attctactag aaaatacaga aatattcaaa tttttatttg
    27181 tcaattttgt gtaaattata tttacagtat cctttacaca aatctgtaaa aagcaaggct
    27241 gatttatttt gataaattac ggttatttca ttaaaaaaat gctataattg aaaggactat
    27301 atcgaaggag aacaaaatga ctaaacccat tattttaaca ggagaccgtc caacaggaaa
    27361 attgcatatt ggacattatg ttggaagtct caaaaatcga gtattattac aggaagagga
    27421 taagtatgat atgtttgtgt tcttggctga ccaacaagcc ttgacagatc atgccaaaga
    27481 tcctcaaacc attgtagagt ctatcggaaa tgtggctttg gattatcttg cagttggatt
    27541 ggatccaaat aagtcaacta tttttattca aagccagatt ccagagttgg ctgagttgtc
    27601 tatgtattat atgaatctag tttcgttagc acgtttggag cgaaatccaa cagtcaagac
    27661 agagatttct cagaaaggat ttggagaaag cattccgaca ggattcttgg tctatccaat
    27721 cgctcaagca gctgatatca cagctttcaa ggctaattat gttcctgttg ggacagatca
    27781 gaaaccaatg attgagcaaa ctcgtgaaat tgttcgttct tttaacaatg catataactg
    27841 tgatgtcttg gtagagccgg aaggtattta tccagaaaat gagagagcag ggcgtttgcc
    27901 tggtttagat ggaaatgcta aaatgtctaa atcactaaat aatggtattt atttagctga
    27961 tgatgcggat actttgcgta aaaaagtaat gagtatgtat acagatccag atcatatccg
    28021 cgttgaggat ccaggtaaga ttgagggaaa tatggttttc cattatctag atgtttttgg
    28081 tcgtccagaa gatgctcaag aaattgctga tatgaaagaa cgttatcaac gaggtggtct
    28141 tggtgatgtg aagaccaagc gttatctact tgaaatatta gaacgtgaac tgggtcctat
    28201 tcgtgagcgc cgtattgaat ttgctaagga tatgggagaa gtttataata tgattcaaaa
    28261 aggtagtgaa agagcgcgtg aagttgcggg tcaaacccta tctgaggtaa aaggggcaat
    28321 gggacttcat tactttaact aagtttattt tacaagaaac tatcttttct atctcgaaga
    28381 aaagatagtt ttttatttac ccctgtaaca tttgacaaat ttttatagaa tggtatgata
    28441 gatacaatat aaaaagagtc aagctcaaaa agaaagaaaa gaggaaactt cgaatgtcta
    28501 attgggacac taaatttttg aaaaaaggtt ttacctttga tgatgtattg cttattccag
    28561 ctgaaagtca tgtgttgcct aacgatgcag atttaacaac taaattggca gataatttga
    28621 ctttaaatat cccaattatt accgctgcca tggacacagt tacagagagt caaatggcca
    28681 ttgctattgc tcgtgcaggc ggtctcggag ttatccataa aaacatgtca attgctcaac
    28741 aagcagacga ggttcgtaag gtaaaacgtt ctgaaaatgg agttattatt gatccgttct
    28801 tcttgacgcc tgaacataca attgctgaag cagatgagct tatgggtcgt taccgcatca
    28861 gtggtgttcc agttgttgaa acacttgaaa atcgtaaatt ggttggtatt ttgacaaacc
    28921 gagatcttcg ttttatttca gattataatc aaccaatttc aaaccatatg actagtgaaa
    28981 atcttgttac tgctcctgtg ggtacggatc ttgcaacggc tgagagtatt cttcaagagc
    29041 atcgtattga aaaacttccg ttggtcgatg aagaaggcag tctttctggt ttgatcacta
    29101 tcaaagatat tgaaaaagtt attgagtttc caaatgcagc taaagatgag tttggtcgtc
    29161 ttctagttgc aggtgcagta ggtgttactt cagatacatt tgaacgtgca gaggctcttt
    29221 ttgaggcagg agcggatgcg attgttattg atactgcaca tggtcattct gcaggtgtct
    29281 tgcgtaaaat tgccgagatt cgtgctcatt tcccagatcg gactttgatt gctggaaata
    29341 ttgctactgc tgaaggtgca cgtgcccttt atgaagcggg tgtagacgtt gttaaggttg
    29401 gtattggacc aggttctatc tgtactactc gtgtgattgc tggtgttggt gttccgcaag
    29461 taacagctat ctacgatgct gcagctgttg cgcgcgaata tggtaaaacg attattgctg
    29521 acggtgggat caagtattct ggagatattg taaaagcact tgctgcaggt ggaaatgctg
    29581 ttatgcttgg atctatgttt gctggaactg atgaagctcc aggcgaaact gaaatcttcc
    29641 aaggacgtaa attcaagact taccgtggta tgggatcaat tgctgctatg aagaaaggtt
    29701 caagcgaccg ttatttccaa ggttctgtca atgaagcaaa caagcttgtt ccagaaggaa
    29761 ttgaaggtcg tgttgcttat aaaggagcgg cagctgatat tgttttccaa atgattggtg
    29821 gtattcgctc tggtatgggt tactgtggtg cagctaacct taaagaacta cacgataatg
    29881 ctcaatttat tgaaatgtct ggtgctggtt tgaaagaaag ccatcctcat gatgtgcaaa
    29941 ttactaatga ggcaccaaat tattctatgt aaaaaacaat gaaaagaact ccagtgaaaa
    30001 caggagttct tttacaatgt tgtcaatttc catttacagc agctttacca tcctgaatag
    30061 tgaagatact tagattttct ggcagatttt gaagatggtc taagcttgtt gttgtgataa
    30121 aggtttggat tgattgagaa atcgtttcta ataattttaa ctgtctagtg ttgtcaagtt
    30181 cactcatcac atcgtcaagc agtaatatag gagattctgt ggtaatgctt tccattaatt
    30241 cgatttctgc taattttatc gagaggacga gactacgatg ttgaccttgg cttccgaaac
    30301 tagcatccat cccatttata taaaaagaaa tgtcatctcg atgaggaccg acaccagtat
    30361 tctttttaaa taaatctctg gatctacttt tttctaaagc aattttgaaa gattcggata
    30421 agttttgttt gtcagttata ttgacagaag attgatagga tattgacaac tcttcgatct
    30481 gattagagag ttcaaaatgt ttcttacgcc caaatgattc tagttttttt atgaaatcta
    30541 agcggtgatt cattacacga catccataat caactagctg atcatctaac acagaaagga
    30601 atgtttcatc tattttttga gctgatttta ggtaagtgtt tctttgcttt aggatgtggt
    30661 tataattggt taagtcagat aaatagattg gcttaatttg cccaagttcc atatcaatga
    30721 attttcgtcg aatcgaaggt gctcctttaa ttagttgtaa atcttcagga gcaaataaga
    30781 caacattcat gtgtcctaca taatctgaaa ggcgtgcctg ttttaagtga ttaacttttg
    30841 ttacacgacc tttttgtgtt agttcgattt ctaggggaat ggatccagtt tttttctgaa
    30901 cgagacctga aagatgaagt tgttcctcat caaaatgaat gagattttta tctgttcgag
    30961 ttcgatgact acgcgttaag gctaaaaaat agatagcctc taacatgttt gtttttcctt
    31021 gtgcattacg tcccaaaaag acattcaatt taggattaaa gtctattttc gtttctttgt
    31081 aattacgaaa agtcttgaga gatagatgtt gtagccacat gattatctac cagggaatcg
    31141 tggagcttgt tggcttttag gtgatgaagc aggtttcgat ttgtcttttt taactccctt
    31201 attcatcttt ttgacaagtt tagcgatccg ttctttttca actttatcag cttggtattc
    31261 atcttgttct tcagaagtag gttgtgtcaa caagatgtca atgttcatgt cagggatgtc
    31321 aactttatcg ccaatacgaa gtttttttcc acgacggctc tctaattccc tattaaagta
    31381 aacagaatgt tcagagagaa atgatttaat agctcctccg ctatgtgtaa ttccaagttc
    31441 tttgagtagt gcttggaggg taataaattc ttcaaataat ttgtattcca taattcacct
    31501 caatctttgg tattatacca tattttccta aaaatagtga gagagacagc gagaaatgta
    31561 agcgattttt atttcaaaag tgttacaggg aacaagaaaa tttcaggttt tcgtgatata
    31621 atagaagtct gtatataagg aggtaaatca tggagttagt gcatggaatt tcaacacatt
    31681 ttatccaatc aaaaaagttt aaaacaaaca aaattaccgt gcgttttacc gctccattat
    31741 cccttgatac gattgcaggt cacatgttga gtgcaagtat gctagagact gctaatcaga
    31801 tgtaccccac ttctcaagat ttgaggagac acttggccag tctatacggt acagatatgt
    31861 caaccaattg tttcagaaga gggcaaagcc acattataga attgacattt acctatgttc
    31921 gtgatgagtt tttaagtagg aaaaacgtgc taacctctca gattttggaa cttgtaaaag
    31981 aaactctttt ttcacccgta gtagttgata atgggtttga tccggcctta tttgaaattg
    32041 agaaaaaaca attgctagca agtttagcag ctgatatgga tgattctttt tattttgcac
    32101 ataaagaatt ggataaattg ttttttcatg atgaacgtct tcaattggaa tatagtgatt
    32161 tacgaaatcg tattttagct gaaactccac aaagttctta ttcttgtttc caagaatttt
    32221 tagccaatga tcgaatagat ttctttttcc taggtgattt taatgaggtt gaaattcaaa
    32281 atgtattaga atcatttggc tttaaaggtc gaaaaggaga tgtgaaggtt cagtattgtc
    32341 aaccttattc taatatcctt caggaaggta tggttcggaa aaatgtggga caatccattt
    32401 tggaattagg ttatcattac cgttctaaat atggtgatga acaacattta cccatgattg
    32461 taatgaatgg tttacttggt ggatttgctc actctaagct ctttacaaat gtccgtgaaa
    32521 atgctggatt agcttatacc atttcaagtg agcttgattt atttagtgga ttcttgagga
    32581 tgtatgctgg tatcaatcga gaaaatcgta accaggctcg taaaatgatg aataatcaac
    32641 tgcttgattt aaaaaaaggt tattttacag agtttgagtt aaatcagacc aaggaaatga
    32701 ttcgttggtc gttgttactt tctcaagata atcaatcttc attgattgaa cgtgcttatc
    32761 aaaatgcctt atttggaaaa tcttcagcag actttaaaag ttggattgca aagcttgaac
    32821 aaattgacaa agatgctatt tgtagagtag ctaataatgt gaaactacaa gcgatttact
    32881 ttatggaagg aatagaatga caaaggttgt ttttgaagaa aaatactatc cagctgtaaa
    32941 agaaatggtt tatcgaactc gtttggccaa cggattgaca gttgctcttt tgcctaaaaa
    33001 ggaatttaaa gaggtttacg ggagtgtcac tgtacagttt ggttcggtag atacgtttgt
    33061 cacagaagtt gacggagatg taaaacaata tcctggagga attgctcatt ttcttgaaca
    33121 taaattattt gagagagaag attctagtga tttgatgtcg gcttttacga gtctaggtgc
    33181 agatagtaat gcctttacaa gctttacaaa aacaaactat cttttttcag caacggatta
    33241 ttttttagaa aatttagatt tacttgatga attggtaaca tcagcacact ttactgaagc
    33301 ttccattctg acagagcagg atattattca gcaagaacga gaaatgtacc aagatgatcc
    33361 agattcgtgt ttattctttt caactttagc gaatttgtat cctggtacac ctttagcaac
    33421 tgatatagtt ggaagtgagg agtccatttc ccaaatcaat ctaactaatt tgcaagaaaa
    33481 ttttacaaag ttttacaaac ctgtaaacat gtctctgttt ttagttggta attttgatgt
    33541 ggagcgagta caggactatt ttgaaagcaa agaactgaaa gattcagatt ttcaggaagt
    33601 agcaagagaa aagttgtttt tacagcctgt aaagccaaca gatagtatga gaatggaagt
    33661 atcttctccc aaactagcga ttggagttag aggtaagcga gaagtttctg aagcggattg
    33721 ctatcgacat catattttat taaaattatt gtttgcaatg atgtttggtt ggacttcgga
    33781 tcgttttcaa aaatgttatg aatcaggtaa aattgatgcg tccttatctc tggaagttga
    33841 aataacaagt cgctttcatt ttgtcatgtt gacaatggat acgaaagagc cagttgcttt
    33901 gtctcatcaa tttaggaagg ctattcgtaa ttttacaaag gatttagata ttacagagga
    33961 acatttagat attatcaaaa gagagatgtt tggcgaattt ttcagtagca tgaactctct
    34021 tgaatttatt gcaacgcaat atgatgcttt tgaaaatggt gagacaattt ttgatttgcc
    34081 gaaaatttta caggaaatta ctttagagga tgtccttgat gctggacatc atttaataga
    34141 tgatggtgac atagttgatt ttacaatatt cccatcgtag taacctatta taatagacac
    34201 tagaaagaag ggatgacaag tatgagaaaa aaaacaattg gagaggtttt acgattagct
    34261 agaatcaatc agggattgag tttagatgaa ttgcagaaaa agacagaaat ccagttagat
    34321 atgttggaag caatggaagc agacgatttc gatcaacttc caagtccttt ttacacgcgt
    34381 tctttcttga aaaaatatgc atgggctgtt gagttagatg accaaattgt tttggatgct
    34441 tatgattctg ggagtatgat tacttatgag gaagtagatg ttgatgaaga tgagttgaca
    34501 ggtcgtagac gttcaagtaa gaaaaagaag aaaaaaatat catttttacc tttattttat
    34561 tttatccttt ttgctttatc gattttaatt tttgtgactt attatgtttg gaactatatt
    34621 caaactcaac cagaggagcc ttctctttct aattacagtg tggttcaatc aacaagttca
    34681 actagctctg ttccccactc ctcaagtagt agttcttcta gtatagaatc agctataagt
    34741 gtatcaggcg aaggaaatca tgtagaaatc gcttataaga caagtaagga aacagttaaa
    34801 ttgcaattgg cagtttcaga tgttacaagt tgggtcagtg tttcagaaag cgaacttgag
    34861 ggcggtgtaa ccttatcgcc aaagaagaaa agtgcagaag caacagttgc aactaaaagt
    34921 cctgtaacaa ttacgttagg tgttgtaaaa ggtgttgatt tgacagtaga taatcagact
    34981 gttgatttat cgaaattaac agctcagact ggacaaatca ctgtaacctt tactaaaaat
    35041 taaggaaaaa cgaatgaaaa aagaacaaat tcccaatctc ttaacaatag gtcgaattct
    35101 ctttatacct atttttatct ttattttaac gataggaaat tcgatagaga gtcatatagt
    35161 tgcagctatt atctttgctg ttgccagtat taccgactat ttagatggat atttagctcg
    35221 taaatggaat gtggtcagta attttggtaa atttgcagat cctatggcgg ataagttact
    35281 agttatgtcg gcttttatta tgttgattga gttaggtatg gctccggctt ggattgttgc
    35341 agtgattatc tgtcgtgagt tagctgtgac aggtttaagg cttttattgg ttgaaactgg
    35401 tggaacaatt ttagcagcag caatgcctgg aaaaattaaa acttttagtc agatgtttgc
    35461 cattattttc ttgctattac attggacttt gcttggtcaa gttctacttt atgtagcctt
    35521 atttttcact atctactctg gctatgacta tttcaagggt agtgcctatg tatttaaagg
    35581 gacatttggt tcgaaatgaa atcaataatt gatgtaaaaa atctttcttt tcgctataaa
    35641 gaaaatcaga actactacga tgtgaaggat attacgtttc acgtgaaacg tggagaatgg
    35701 ctttcgattg tagggcataa tggtagtggt aaatcaacga cggttcgatt aattgatggc
    35761 ttactggaag cagaatccgg agagattgta attgatggcc aacgtttgac tgaggaaaat
    35821 gtttggaata tacgtcgtca aatcggtatg gtttttcaaa atccagacaa tcaatttgtt
    35881 ggagcgactg ttgaagatga tgttgctttt ggtttggaaa atcagggact ttctcgtcaa
    35941 gaaatgaaaa agagagtgga agaagctctg gctttagttg gcatgttgga ctttaaaaag
    36001 agagagccag cgcgtctatc aggtggccaa aagcaacgtg tggccattgc aggtgttgta
    36061 gccctaagac cagctatttt aatcttagat gaagcaacga gtatgttgga tcctgagggg
    36121 cgtagagaac ttattgggac agtaaaagga attcgaaaag actatgatat gacggtcatt
    36181 tctattaccc atgatttgga agaagtcgcc atgagtgatc gcgtattggt catgaaaaaa
    36241 ggggaaattg aatcaactag tagtccaagg gagcttttct ctcgaaatga tttagatcaa
    36301 attggattag acgatccttt tgctaatcaa ttaaaaaaat ctttgagcca gaatggctat
    36361 gatttacctg aaaattattt gacagaaagt gagctagagg ataagctatg ggaattgctc
    36421 tagaaaatgt gaattttaca tatcaagaag gtactccctt agcttcagca gctttgtcgg
    36481 atgtttcttt gacgattgaa gatggctctt atacagcttt aattgggcac acaggtagtg
    36541 gtaaatcaac tattttacaa ctcttaaatg gtttattggt gccaagtcaa gggagtgtga
    36601 gggtttttga taccttaatc acctcgactt ctaaaaataa agatattcgt caaattagaa
    36661 aacaggttgg cttggtattt cagtttgctg aaaatcagat ttttgaagaa acggttttga
    36721 aggacgttgc ttttggaccg caaaattttg gagtttctga agaagatgct gtgaagactg
    36781 cgcgtgagaa actggctctg gttggaattg atgaatcact ttttgatcgt agtccgtttg
    36841 agctgtcagg gggacaaatg agacgtgttg ccattgcagg catacttgcc atggagccag
    36901 ctatattagt cttagatgag ccaacagctg gtctagatcc tctagggaga aaagagttga
    36961 tgaccctgtt caaaaaactc caccagtcag ggatgaccat cgtcttggta acgcatttga
    37021 tggatgatgt tgctgaatat gcgaatcaag tctatgtaat ggaaaaggga cgtttagtaa
    37081 aggggggcaa accaagtgat gtctttcaag acgttgtttt tatggaagaa gttcagttgg
    37141 gagtacctaa aattacggcc ttttgtaaac gattggctga tagaggcgtg tcatttaaac
    37201 gattaccgat taagatagag gagttcaagg agtcgctaaa tggatagtat gattttaggg
    37261 cgttatatcc caggggattc gattgttcac cgattggatc cacgtagcaa attgcttgct
    37321 atgatgctac tgattttgat tgtgttttgg gctaataacc ccttgacgaa tctgattctt
    37381 tttatagcga cagggatatt tattgccttg tcaggagtat ctctttcatt ttttattcag
    37441 ggcttgaagt ctatgttttt cttgattgcc ttcacaacta tttttcaact atttttcatt
    37501 tctaatggga atgttttatt tgagttttcg tttgtgagaa tcacggatta tgctttgcaa
    37561 caagctggga ttattttttg tcgctttgta ttgattattt tcttttcaac tttgttaacc
    37621 ttaacaacca tgcctttaag tttggcatca gctgtcgaag ctttattagc acctttaaag
    37681 cgtgtgaaag ttccagttca tgaaattgga ttgatgctgt ctatgagttt gcgttttgtc
    37741 ccaaccttga tggatgatac gactcggatt atgaatgcac agaaagctcg tggagtggat
    37801 tttggagaag gaagcatcgt tcaaaaagta aaggcgatga ttcccatttt gattcctctt
    37861 tttgcgacaa gtttaaaacg tgcagattcc ttggctatcg ctatggaagc gcgtggctat
    37921 cagggtggaa aaggcagaag tcaataccga caattgaaat ggactctgaa ggatacgctg
    37981 accattcttg ttattctcgt acttggttgt tgtttatttt tcttaaaatc ttagtagctt
    38041 tcaggaattg ataaaaagtt actgtaacag tttttgatat aaaggtagga atatgaaccg
    38101 ttttaaaaaa tcaaaatatg tcattattgt ttttgtcact gttctgcttg tgtcagctct
    38161 cttagcgacg acttattcaa gtacaattgt gacaaaatta ggagatggaa tctcattggt
    38221 tgatagagtt gtacaaaaac cttttcagtg gtttgattct gtcaaatcag atttggctca
    38281 tttgacacga acatataatg aaaatgaaag tttgaagaaa cagctttacc aattagaagt
    38341 taaatcaaat gaggtggaaa gtttaaagac agaaaatgaa caactgcgcc aattgcttga
    38401 tatgaagtct aaattgcaag ccacaaagac tttagcagca gatgttatta tgcgttctcc
    38461 ggtatcttgg aagcaggagt tgaccttaga tgcaggtaga tcaaaaggtg cttctgagaa
    38521 catgttagct attgcaaatg gtggcttgat tgggagtgtt tcaaaagtag aggagaactc
    38581 tactatagtc aaccttctga caaatacgga aaatgctgat aagatttctg ttaaaattca
    38641 acatggctct actacaattt atggaattat tattggctat gacaaggaaa atgacgttct
    38701 taaaattagc caattaaata gtaatagcga tattagtgcg ggagataagg tgactactgg
    38761 tggattagga aactttaacg ttgctgatat tcctgttggt gaagtggttg ccacaacgca
    38821 tagtacagac tatttgacac gagaagtaac tgttaaattg agtgcagata ctcataatgt
    38881 agatgtgata gaattagtgg ggaattcata atgagacagt tgaagcgagt tggagtattt
    38941 ttattgcttc ctttctttgt tctaattgac gcccatatta gccagcttct gggctcattt
    39001 ttcccccatg tacatttggc tagtcatttt ctttttctat ttctcttatt tgagacgata
    39061 gaagtatcag agtatctcta cctagtctat tgttttgtta taggcttggt ttatgatgtt
    39121 tactttttcc atctaatagg gattacaact ctcttattta tcttattggg agccttcctt
    39181 cataaattga atagtgttat tttgttgaat cgttggacaa gaatgctagc tatgattgtg
    39241 ctgacattcc tgtttgaaat gggtagttat cttttggctt ttatggtagg gttgacagta
    39301 gatagcatgt cgatttttat agtctatagc ttggtaccga cgatgatttt aaatttttta
    39361 tggattactg tttttcaatt tatttttgaa aaatattatc tataagaacg acatataaat
    39421 gtaacaaagg cgtaatattt attaggcctt tttttggtat actagtattg tctttaaaag
    39481 aaggagtatc tacgtaatat gaagaaaaaa atcttagcgt cacttttatt aagtacagta
    39541 atggtttctc aagtagctgt tttaacaact gcgcatgcag aaacgactga tgacaaaatt
    39601 gctgctcaag ataataaaat tagtaactta acagcacaac aacaagaagc ccaaaaacaa
    39661 gttgaccaaa ttcaggagca agtatcagct attcaagctg agcagtctaa cttgcaagct
    39721 gaaaatgata gattacaagc agaatctaag aaactcgagg gtgagattac agaactttct
    39781 aaaaacattg tttctcgtaa ccaatcgttg gaaaaacaag ctcgtagtgc tcaaacaaat
    39841 ggagccgtaa ctagctatat caataccatt gtaaactcaa aatcaattac agaagctatt
    39901 tcacgtgttg ctgcaatgag tgaaatcgta tctgcaaaca acaaaatgtt agaacaacaa
    39961 aaggcagata aaaaagctat ttctgaaaaa caagtagcaa ataatgatgc tatcaatact
    40021 gtaattgcta atcaacaaaa attggctgat gatgctcaag cgttgactac gaaacaggca
    40081 gaactaaaag ctgctgaatt aagtcttgct gctgagaaag cgacagctga aggggaaaaa
    40141 gcaagtctat tagagcaaaa agcagcagct gaggcagagg ctcgtgcagc tgcggtagca
    40201 gaagcagctt ataaagaaaa acgagctagc caacaacaat cagtacttgc ttcagcaaac
    40261 actaacttaa cagctcaagt gcaagcagta tctgaatctg cagcagcacc tgtccgtgca
    40321 aaagttcgtc caacatacag tacaaacgct tcaagttatc caattggaga atgtacatgg
    40381 ggagtaaaaa cattggcacc ttgggctgga gactactggg gtaatggagc acagtgggct
    40441 acaagtgcag cagcagcagg tttccgtaca ggttcaacac ctcaagttgg agcaattgca
    40501 tgttggaatg atggtggata tggtcacgta gcggttgtta cagctgttga atcaacaaca
    40561 cgtatccaag tatcagaatc aaattatgca ggtaatcgta caattggaaa tcaccgtgga
    40621 tggttcaatc caacaacaac ttctgaaggt tttgttacat atatttatgc agattaattt
    40681 acagagggac tcgaatagag ccctcttttc aggttttacc gtgacaatcc ctattaaaaa
    40741 ttatatcaaa atagcttgaa aatattggaa aagtatggta aaatgaaaat tgtcgtgtga
    40801 acgataatac tcattcttga tgaattgtga agcagttgcc cttgggtcgt tttgcgagtt
    40861 gaagtcaaga agaggaaaaa aacaaaaagg agaaatactc atggcagtaa tttcaatgaa
    40921 acaacttctt gaggctggtg tacactttgg tcaccaaact cgtcgctgga atcctaagat
    40981 ggctaagtac atctttactg aacgtaacgg aatccacgtt atcgacttgc aacaaactgt
    41041 aaaatacgct gaccaagcat acgacttcat gcgtgatgca gcagctaacg atgcagttgt
    41101 attgttcgtt ggtactaaga aacaagcagc tgatgcagtt gctgaagaag cagtacgttc
    41161 aggtcaatac ttcatcaacc accgttggtt gggtggaact cttacaaact ggggaacaat
    41221 ccaaaaacgt atcgctcgtt tgaaagaaat taaacgtatg gaagaagatg gaactttcga
    41281 agttcttcct aagaaagaag ttgcacttct taacaaacaa cgtgcgcgtc ttgaaaaatt
    41341 cttgggcggt atcgaagata tgcctcgtat cccagatgtg atgtacgtag ttgacccaca
    41401 taaagagcaa atcgctgtta aagaagctaa aaaattggga atcccagttg tagcgatggt
    41461 tgacaccaat actgatccag atgatatcga tgtaatcatc ccagctaacg atgacgctat
    41521 ccgtgctgtt aaattgatca cagctaaatt ggctgacgct attatcgaag gacgtcaagg
    41581 tgaggatgca gtagcagttg aagcagaatt tgcagcttca gaaactcaag cagattcaat
    41641 tgaagaaatc gttgaagttg tagaaggtga caacgcttaa tttatacaaa tagtaattac
    41701 ctaggagggc ggggcttagc ccggctctcc tattttcaaa aaatatagga gaattaaaat
    41761 ggcagaaatt acagctaaac ttgtaaaaga gttgcgtgaa aaatctggtg ccggtgttat
    41821 ggacgctaaa aaagcgcttg tagaaacaga cggtgacatc gaaaaagcga ttgaattgct
    41881 tcgtgaaaaa ggtatggcta aggcagctaa gaaagctgac cgtgttgctg cagaaggttt
    41941 gactggtgtt tatgttaacg gtaatgttgc agcagttatt gaagtaaacg ctgaaactga
    42001 cttcgttgca aaaaacgctc aattcgttga attggtaaat actacagcta aagtcattgc
    42061 tgaaggaaaa cctgctaaca atgaagaagc tcttgctttg ataatgcctt caggtgaaac
    42121 tcttgaagct gcatacgtat ctgcaacagc aactatcgga gagaaaatct cattccgtcg
    42181 ctttgcattg attgaaaaaa cagacgcaca acactttgga gcataccaac ataacggtgg
    42241 acgtatcggt gttatttcag ttgttgaagg tggagacgaa gcacttgcta aacaattgtc
    42301 aatgcacatc gcagcgatga aaccaacagt tctttcttac aaagaattgg atgagcaatt
    42361 cgttaaagat gagttggcac aattgaatca cgttatcgac caagacaacg aaagccgtgc
    42421 aatggttaat aaaccagctc ttccacactt gaagtatgga tcaaaagctc aattaactga
    42481 tgatgttatt gctcaagctg aagctgacat caaagctgaa ttggctgcag aaggcaaacc
    42541 agaaaaaatc tgggacaaaa ttattccagg taaaatggat cgcttcatgc ttgataacac
    42601 taaagttgac caagcttaca cacttcttgc acaagtttac atcatggatg acagcaagac
    42661 agttgaagca taccttgaat cagttaacgc ttcggtagtt gagtttgctc gctttgaagt
    42721 tggtgaaggt atcgagaaag ctgcaaacga cttcgaagct gaagttgcag ctacaatggc
    42781 agcagccttg aataactaat aatagaaagg aagcaaattg cttccttttt tgtatagggc
    42841 ttagattttt cctgaaattg ttttttcccc agtctcactc gattagaata attaaaacaa
    42901 aaagccctat aataagggct aagggcattt aggagactat acttcaaatt catagtaagc
    42961 gcgttcataa caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata
    43021 attatcggag gaaaggtatt gtactatgat aattcaacta agtgatttag gtcaagttca
    43081 ccttgtttgt agcaagatag atatgaggca gggaatagac tcattagcct atgtagttaa
    43141 aacccacttt gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa
    43201 agaccgcttt aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt
    43261 tgagaacgtc agactgactt ggcccagtac agaaaaggat gtcaaagctc tcacacctga
    43321 acaagtagac tggcttatga agggcttttc tatcactcca aaaatatagt agattgaaac
    43381 tagaatagta cacctctgct tctaaaacat tgttagaaat cgatttgact gtcctgatcg
    43441 atttgtcctg ttcttatttc attttactat aaatccatca gaaagtcgtg atttctattg
    43501 aaatgaggac tttcttttta tactcatctg ttttcaaaaa gcattctagt ccatctccga
    43561 ttaacgatgg actttatcac ctccttctcc agtccttgta taacatcttg gagttgattc
    43621 atgacatctt ccaaagtttg aaaggtttta ttcttaaatc cacgtttacg aatctctttc
    43681 cacacttgtt caatggggtt catctctggt gtgtatggag gaataaatgc aaagccaata
    43741 ttagtcggaa tctttaaggt acttgattta tgccatatag cattgtccat aacgagtaaa
    43801 agataatcat ctggataagc ttgtgaaatc tcctattcct aaagcccctt tagcgcataa
    43861 ctttggctca gcttctatta tcgctcacac catccatcag aagtttaatc tgaaggtacc
    43921 caattatcgc caagaagaag attgggctag gatgggttta ccaatcacac gtaaggaaat
    43981 ctctaatcgg catatcaaga cgagtcaata ctatttggag cccctttata acctcttgcg
    44041 agagagacta ttgactcagc ccttacttca tgcggatgaa acttcttata gggtgctaga
    44101 gagtgatagt cagctgactt actattggac ttttttgtct gggaaagctg agaaacaagg
    44161 gattacgctt taccatcatg tcctaatcga tttgttcata tcttatttca atccattata
    44221 aatagtgaga aatatctatc ctatcttcta gaatgtcttc caaacgtgga aactctcgta
    44281 aacaaagagg ttttagaggc ctatttacca tggactaaag ttgtacaaga aaagtgcaaa
    44341 taagaaatct ccagattagg aactatccgt gagttctcta gtctggagat ttttcaatag
    44401 acttcgttat tggacggtta caattcataa agtgctgtag agagataacg ttcaccgtta
    44461 tctggtgcta gggcaaggac ttttttacct gtacctaatt ttttggcaac ctcgatggct
    44521 ccgtagatag ctgcagctga ggaaatccct acaaggaagc cttcttttcc accaatttca
    44581 cgtccgagtg caagagcgtc atctgatgtt acacgaacga taccatcata ggctttagta
    44641 tcaagtgtat caggaataaa tccagctgag ataccttgaa ttttgtgagg accaggtttt
    44701 tcaccagata gaatagcaga ttcatctgct tctactgcaa aaacttgaat gttagaattt
    44761 tctgatttga gtgcatgaga aacaccagaa atcgttccac cagtacctac tccagcaaca
    44821 aaggcatcta atccatcttt accgaaagca gctagtatct cagctcctgt tgttctttcg
    44881 tgtacttctg gattagctgg attgtcaaat tgaagaggaa ggaaaccatc acgttcagca
    44941 gcgatttctt gagccttagc aatagcacct ttcattccct cgctaccagg agttaggacg
    45001 agttcagcac cataagcttg gataatttta cgtcgttcta cactcatagt ttcaggcata
    45061 acgatgacga ctttataccc tttagcagca cctacccatg aaagtccaat accggtgttt
    45121 ccacttgttg cttcaacaat agtagaacca agtttcagaa taccatcttg ttcagctttt
    45181 tcaatcatgc taagggcaat acggtctttt acagatgaac caggattaaa tgcttcaagc
    45241 tttatataga cgtctgcagc accttctggc acgatgttgt taagtttaac aatcggtgtt
    45301 tgaccgatta attcagtaat gttgttataa atagacatat gaatacctct ttgtataaga
    45361 tatctttagt ataacgctat ccataccatt tgtaaaatat agaaatccta tcgaagcgat
    45421 aggatttcta tatatcatag atcaaagtca ttaattttca agtgattgcg ttcagttaat
    45481 tctagtaaat aggtgtgaag gaaaacgata tcaggtttcg tttgaattgc ttcattttct
    45541 agtatatttt ggtacgcatc tttttatttt gctcgctaaa gtagttgtga gaatgtcaac
    45601 gaaacgctgt attattcatt gtttacacta agtttacagg aacttcaacc tctcgtaaac
    45661 cttggtcagt taaagtgact tttccattaa aaaactccac aagtgcagct ttaatagttt
    45721 ctttttcttc tttatcaaca taaatcatcg tatcgacttg atctgtaaag tttgtatcca
    45781 gctccatgag atcatgttct ttaaggaagt tactgtactc ttggtactga gcataagaca
    45841 tttgaatagc aatgccagcc tgttctttta tttcaataat accaatttct ttgacagcta
    45901 aggcgacact gccggcgtaa gcacgaatta gtcctccagc gcctagttta ataccaccaa
    45961 agtagcgtgt cacgaccaca cagacattgg tgagattgtg attttctagt accccaagca
    46021 tgggaacacc agcagtacca ctaggctcac catcatcact tgtacgttta atttcactac
    46081 gttctccaat aataaaggca gagcagttat gtgtcgcttt gtagtgttct tttttgatgg
    46141 cagtaatgaa gtcacgagcc tcttcttcgc tataaacacg cttggcatgg cagataaagc
    46201 gagatttttt gatttcttct tggacttggc cgtcctcttt aattgtccta aattccatga
    46261 tttaattgta ctaaaaaatc atctaaagtg ctagttttta cgaataaaga agtatgaaag
    46321 taaatttaga ttatctcggt cgtttattta ctgagaatga attaacagaa gaagaacgtc
    46381 agttggcgga gaaacttcca gcaatgagaa aggagaaggg gaaacttttc tgtcaacgtt
    46441 gtaatagcac tattctagca gaatggtatt tgcccatcgg tgcttactat tgtcgagagt
    46501 gcttgctgat gaagcgagtc agaagtgatc aaactttata ctattttccg caggaggatt
    46561 ttccgaagca agatgttctc aaatggcgcg gccaattaac tccttttcaa gagaaggtgt
    46621 cagagggact gcttcaagca gtagacaagc aaaagccaac cttagttcat gcggtaacag
    46681 gagctggaaa gacagaaatg atttatcaag tagtggctaa agtgatcaat gcgggtggtg
    46741 cagtgtgttt ggctagtcct cgcatagatg tttgtttgga gctgtacaag cgcctgcaac
    46801 aggatttttc ttgcgggata gctttgctac atggagaatc ggaaccttat tttcgaacac
    46861 cactagttgt tgcaacaacc catcagttat tgaagtttta tcaagctttt gatttgctga
    46921 tagtggatga agtagatgct tttccttatg ttgataatcc cacgctttac catgctgtca
    46981 agaatagtgt aaaggagaat ggattgagaa tctttttaac agcgacttcg accaatgagt
    47041 tagataaaaa ggtccgttta ggagaactaa aaagactgaa tttaccgaga cggtttcatg
    47101 gaaatccgtt gattattcca aaaccaattt ggttatcgga ttttaatcgc tacttagaca
    47161 agaatcgttt gtcaccaaag ttaaagtcct atattgagaa gcagagaaag acagcttatc
    47221 cgttactcat ttttgcttca gaaattaaga aaggggagca gttagaagaa atcttacagg
    47281 agcaatttcc aaatgagaaa attggctttg tatcttctgt aacagaggat cgattagagc
    47341 aagtacaagc ttttcgagat ggagaactga caatacttat cagtacgaca atcttggagc
    47401 gtggagttac cttcccttgt gtggatgttt tcgtagtaga ggccaatcat cgtttgttta
    47461 ccaagtctag tttgattcag attggtggac gagttggacg aagcatggat agaccgacag
    47521 gagatttgct tttcttccat gatgggttaa atgcttcaat caagaaggcg attaaggaaa
    47581 ttcagatgat gaataaggag gctggtctat gaagtgcttg ttatgtgggc agactatgaa
    47641 gactgtttta acttttagta gtctcttact tctgaggaat gatgactctt gtctttgttc
    47701 agactgtgat tctacttttg aaagaattgg ggaagagaac tgtccaaatt gtatgaaaac
    47761 agagttgtca acaaagtgtc aagattgtca actttggtgt aaagaaggag ttgaagtcag
    47821 tcatagagcg atttttactt acaatcaagc tatgaaggat tttttcagtc ggtataagtt
    47881 tgatggagac ttcctgttaa gaaaagtttt cgcttcattt ttaagtgagg agttgaaaaa
    47941 gtacaaagag tatcaatttg ttgtaattcc cctaagtcct gatagatatg ctaatagagg
    48001 atttaatcag gttgagggct tggtagaggc agcaggcttt gagtatctgg atttattaga
    48061 gaaaagagaa gagagagcca gttcttctaa aaatcgttca gagcgcttgg ggacagaact
    48121 tcctttcttt attaaaagtg gagtcactat tcctaaaaaa atcctactta tagatgatat
    48181 ctatactaca ggagcaacta taaatcgtgt taagaaactg ttggaagaag ctggtgctaa
    48241 ggatgtaaaa acattttccc ttgtaagatg aggaaaaaat ttgcaaaaat agaatgaaag
    48301 cgttataata aaatcataaa aacaaaaatg tttagaaaga aggtactcat atgattaaat
    48361 atagtatccg tggtgaaaac ctagaagtaa cagaagcaat tcgtgattat gtagtttcta
    48421 aactcgaaaa gatcgaaaag tacttccaac cagaacaaga gttggatgcc cgaattaact
    48481 taaaagttta tcgtgaaaaa acggctaaag tggaagtaac gattccgctt ggatctatta
    48541 ctctccgcgc agaagatgta tctcaagata tgtatggttc aattgacctt gtaactgata
    48601 aaattgaacg tcagattcgt aaaaataaaa caaaaatcga gcgtaaaaat aaaaataagg
    48661 tagcaactgg tcaattattt acagatgctt tggtggaaga ttcaaatatt gtccagtcta
    48721 aagttgttcg ttcaaaacaa attgatttaa aaccaatgga tttggaagaa gcaattctac
    48781 aaatggattt attggggcat gatttcttta tctatgtgga tgttgaagat cagacaacca
    48841 atgtgattta tcgtcgtgag gatggcgaaa ttggtttgtt agaggttaaa gaatcttaat
    48901 ttcaatatgt gtaaaggtag gtttactgaa ttgtaaacta cctttttctt ataattgata
    48961 gaattactga ttacactttt aaaaagtcgc tttttgaggg gtattatggt ataatgggtg
    49021 ccaagaggtt ttgaatgaaa aaattttatg taagtccaat ttttcctatt ctagtaggat
    49081 tgattgcgtt tggagtctta tccactttca ttatttttgt taataataat ctgttgacgg
    49141 ttttaatttt gtttcttttt gtaggaggct atgttttttt atttaagaaa ctgagagtgc
    49201 attatacaag gagtgatgta gaacagatac agtatgtaaa ccaccaagcg gaagaaagtt
    49261 tgacagctct attggaacag atgcctgtag gtgttatgaa attgaattta tcttctggag
    49321 aggttgagtg gtttaatccc tatgctgaat tgattttgac caaggaagat ggtgattttg
    49381 atttagaagc tgttcaaacg attatcaagg cttcagtagg aaatccgtct acttatgcca
    49441 agcttggtga gaagcgttat gctgttcata tggatgcttc ttccggtgtt ttgtattttg
    49501 tagatgtatc cagggaacaa gccataacag atgaattggt aacaagtaga ccagtgattg
    49561 ggattgtctc tgtggataat tatgatgatt tggaggatgc aacttctgag tcagatatta
    49621 gtcaaatcaa tagttttgta gctaatttta tatcagagtt ttcagaaaaa tacatgatgt
    49681 tttctcgtcg ggtaagtatg gatcgatttt atctatttac tgactacacg gtgcttgagg
    49741 gcttgatgaa tgataaattt tctgttattg atgctttcag agaagagtcg aaacagagac
    49801 agttgccctt gaccttaagt atgggatttt cttatggcga tggaaatcat gatgagatag
    49861 ggaaagttgc tttgctcaat ttgaacttgg ctgaagtacg tggtggcgac caggtggttg
    49921 ttaaggaaaa cgacgaaacg aaaaatccag tttattttgg tggtgggtct gctgcttcaa
    49981 tcaagcgtac acggactcgt acgcgcgcta tgatgacagc tatttcagat aagattcgga
    50041 gtgtagatca ggtttttgta gtcggtcaca aaaatttaga catggatgct ttgggctctg
    50101 ctgtaggtat gcagttgttc gccagcaatg tgattgaaaa tagctatgct ctttatgatg
    50161 aagaacaaat gtctccagat attgaacgag ctgtttcatt catagaaaaa gaaggagtta
    50221 cgaagttgtt gtctgttaag gatgcaatgg ggatggtgac caatcgttct ttgttgattc
    50281 ttgtagacca ttcaaagaca gccttaacat tatcaaaaga attttatgat ttatttaccc
    50341 aaaccattgt tattgaccac catagaaggg atcaggattt tccagataat gcggttatta
    50401 cttatatcga aagtggtgca agtagtgcca gtgagttggt aacggaattg attcagttcc
    50461 agaattctaa gaaaaatcgt ttgagtcgta tgcaagcaag tgttttgatg gctggtatga
    50521 tgttggatac taaaaatttc acctcgcgag taactagtcg gacatttgat gttgctagct
    50581 atctcagaac gcgcggaagt gatagtattg ctatccagga aatcgctgcg acagattttg
    50641 aagaatatcg tgaggtcaat gaacttattt tacaggggtg taaattaggt tcagatgtac
    50701 taatagcaga ggctaaggac acgaaatgct atgatacagt tgttattagt aaggcagcag
    50761 atgccatgtt agccatgtca ggtattgaag cgagttttgt tcttgcgaag aatacacaag
    50821 gatttatctc tatctcagct cgaagtcgta gtaaactgaa tgtacaacgg attatggaag
    50881 agttaggcgg tggaggccac tttaatttgg cagcagctca aattaaagat gtaaccttgt
    50941 cagaagcagg tgaaaaactg acagaaattg tattaaatga aataaaggaa aaggagaaag
    51001 aagaatgaaa gtaatctttt tagcagatgt taaaggaaaa ggtaaaaaag gcgaaattaa
    51061 ggaagtacca acagggtatg cgcaaaactt tcttatcaaa aagaatctag ccaaagaagc
    51121 gactgctcaa gctgtaggtg aacttcgtgg taaacaaaaa tcggaagaaa aagctcacgc
    51181 tgagatgatt gcagaaggaa aagcaattaa agcacaactt gaagcagaag aaactgttgt
    51241 agaatttgtt gaaaaagttg gtccagatgg tcgtaccttt ggttctatta ccaataagaa
    51301 gattgcagaa gaattgcaaa agcaatttgg aattaagatt gataaacgtc atattcaagt
    51361 acaagctccg attcgagcgg ttggtttgat tgatgtgcca gtgaaaatct atcaagatat
    51421 cacaagtgta atcaatcttc gtgtgaaaga aggataagtt tacaccttct tgacaagatt
    51481 gtaaaaggaa gggaagtctg atggcagaag tagaagagtt acgagtacaa cctcaagata
    51541 tcttagctga gcaatccgtt ttaggggcta tctttattga tgagagtaaa cttgtttttg
    51601 tgcgagaata cattgagtct cgggactttt ttaagtatgc ccatcgtttg attttccaag
    51661 ccatggtcga tttatccgat cgtggtgatg ccatagatgc aacaacggtt cgtactatcc
    51721 ttgataatca aggtgattta cagaatattg gtggcttgtc ttacttggtt gagattgtca
    51781 attctgtgcc aacttctgct aatgcggagt attatgctaa gattgttgca gaaaaagcaa
    51841 tgctacgtcg tttaattgcc aagttgacag agtctgtcaa ccaagcttac gaagcgtcac
    51901 aaccagctga tgaaattatt gctcaggcag aaaaagggct gattgatgtc agtgaaaatg
    51961 caaatcgaag cgggtttaag aacattcgag atgtgttgaa tctcaacttt ggaaatctgg
    52021 aagctcgctc gcaacaaacg accgatatta caggtattgc gacaggttat cgtgatttgg
    52081 atcatatgac aacaggactt catgaggagg agttgattat cttagcagct cgtccagcag
    52141 ttggtaagac agcatttgcc ttgaatatcg ctcagaatat tgggactaag ttggacaaaa
    52201 cggttgctat tttttcactc gaaatgggtg cggaaagctt ggtagaccgt atgttagctg
    52261 cagaaggctt ggtggagtca cattctatcc gtacagggca attgacagat gaggagtggc
    52321 aaaaatatac tattgctcag ggtaatctag ctaacgccag tatctatatc gatgatacgc
    52381 caggtattcg gattacagag attcgttctc gttctcgtaa attggctcaa gaaactggaa
    52441 atcttggttt gattttgata gactatttgc aacttatcac gggaactggt cgagaaaatc
    52501 gtcaacaaga agtttctgaa atttctcgtc agttgaaaat actagccaag gaattgaagg
    52561 ttccagtaat cgctctgagt cagctttctc gtggtgtaga acaacgtcag gacaagagac
    52621 cggtcttgtc tgatattcgt gaatctgggt ctattgagca ggacgctgat atcgtagctt
    52681 ttctctatcg cgatgactac tatgaacgtg gtggtgaaga agaggagggt atcccaaata
    52741 ataaggtgga agttattatc gagaaaaacc gtagtggagc tcgtggaaca gtggaattga
    52801 ttttccaaaa agaatacaat aaattttcaa gtatctcaaa gagggaggca taagatgtca
    52861 gatgcattta cagatgtagc caagatgaaa aaaatcaaag aagaaatcaa ggcacatgag
    52921 ggacaagtcg tagaaatgac tttggagaat ggtcgtaagc gccaaaaaaa tagattgggt
    52981 aagctaattg aagtttatcc atccctattt attgtggagt ttggggatgt ggaaggagat
    53041 aaacaagtta atgtttacgt tgaatccttt acttactcag atattcttac agaaaagaat
    53101 ttgattcatt atcttgacta aagtgagaaa ttttctcact ttttcttttt tctccgaata
    53161 atttaggtga aggcaatcat cgctttatat tattttttaa ggaggaagaa tgaaaatttt
    53221 accgtttata gcaagaggaa caagttatta cttgaagatg tcagttaaaa agcttgttcc
    53281 ttttttagta gtaggattga tgctagcagc tggtgatagt gtctatgcct attccagagg
    53341 aaatggatcg attgcgcgtg gggatgatta tcctgcttat tataaaaatg ggagccagga
    53401 gattgatcag tggcgcatgt attctcgtca gtgtacttct tttgtagcct ttcgtttgag
    53461 taatgtcaat ggttttgaaa ttccggcagc ttatggaaat gcgaatgaat ggggacatcg
    53521 tgctcgtcgg gaaggttatc gtgtagataa tacaccgacg attggttcca ttacttggtc
    53581 tactgcagga acttatggtc atgttgcctg ggtgtcaaat gtaatgggag atcagattga
    53641 gattgaggaa tataactatg gttatacaga atcctataat aaacgaatta taaaagcaaa
    53701 cacgatgaca ggatttattc attttaaaga tttggatggt ggcagtgttg ggaatagtca
    53761 atcctcaact tcaacaggcg gaactcatta ttttaagacc aagtctgcta ttaaaactga
    53821 acccctagtt agtgcaactg tgattgatta ctattatcct ggagagaagg ttcattatga
    53881 tcaaattctc gaaaaagacg gctacaagtg gttgagttat actgcctata atggaagcta
    53941 tcgttatgtt caattggagg ctgtgaataa aaatcctcta ggtaattctg ttctttcttc
    54001 aacaggtgga actcattatt ttaagaccaa gtctgctatc aaaactgaac ccctagttag
    54061 tgcaactgtg attgattact attatcctgg ggagaaggtt cattatgatc agatacttga
    54121 aaaagacggc tacaagtggt tgagttatac ggcttataac ggaagtcgtc gctatataca
    54181 gctagaggga gtgacttctt cacaaaatta tcagaatcaa tcaggaaata tctctagcta
    54241 tggatccaat aatagttcaa ctgtcggttg gaagaaaata aatggtagtt ggtatcattt
    54301 caaatcaaat ggttctaaat caacaggatg gctgaaagat ggttctagct ggtattattt
    54361 gaaattatct ggtgaaatgc agacaggatg gttaaaggag aatggctcgt ggtattatct
    54421 gggtagttca ggggcaatga aaacaggctg gtaccaggtc tctggtaagt ggtattattc
    54481 ttactcttca ggcgccttag ctgttaatac gacggtggat ggctacagag taaacagtga
    54541 tggagaacga gtatagaaaa ttggagtagg agaaatttcc tgctcttttc tgtaagcagt
    54601 ttccttgaca tttttcttat tttgcgctat catatataca tataatatat gggagtctgt
    54661 gtacagtctg agaggaagtg ttaaacttcg accgcacctg atctgggtaa tgccagcgga
    54721 gggaacgata cttagtctaa ttttgcacct tttccatgta tggtaaaggt ttttcttttt
    54781 ttaaaaagga aaacgagaag aggaggttct tatgaaagca agcattgcct tgcaagtttt
    54841 acccctagca caggggattg atcggatagc tgttattgat caggtcattg cttatctgca
    54901 aactcaagaa gtgacgatgg tagtgacacc atttgaaacg gtcttggaag gggagtttga
    54961 tgagcttatg cgcattctaa aagaagcgct ggaagtggca gggcaggagg cagacaatgt
    55021 ctttgccaat gtcaaaataa atgtaggaga gattttaagt attgatgaga aacttgagaa
    55081 gtatactgag acgccacatt agtctattgg gctttctcgg agtcttgtca atctggcagt
    55141 tagcaggttt tcttaaactt ctccccaagt ttatcctgcc gacacctctt gaaattctcc
    55201 agccctttgt tcgtgacaga gaatttctct ggcaccatag ctgggcgacc ttgagagtgg
    55261 ctttactggg gctgattttg ggagttttga ttgcctgtct tatggctgtg ctcatggata
    55321 gtttgacttg gctcaatgac ctgatttacc ctatgatggt ggttattcag accattccga
    55381 ccattgccat agctcctatc ctggtcttgt ggctaggtta tgggattttg cccaagattg
    55441 tcttgattat cttaacgaca acttttccca tcatcgtcag cattttggac ggttttaggc
    55501 attgcgacaa ggatatgctg accttgttta gtctgatgcg ggccaagcct tggcaaatcc
    55561 tgtggcattt taaaattcca gtcagcctgc cctactttta tgctggtctg agggtcagtg
    55621 tctcctacgc ttttatcaca acagtggtat ctgagtggtt gggaggtttt gaaggtcttg
    55681 gtgtttatat gattcagtct aaaaaactgt ttcagtatga taccatgttt gccattatta
    55741 ttctggtgtc gattatcagt cttttgggta tgaagctggt cgatatcagt gaaaaatatg
    55801 tgattaaatg gaaacgtact taaaatagag cgtttttgaa aaagaaaaga ggaaatcaaa
    55861 atgaagaaaa catggaaagt gtttttaacg cttgtaacag ctcttgtagc tgttgtgctt
    55921 gtggcctgtg gtcaaggaac tgcttctaaa gacaacaaag aggcagaact taagaaggtt
    55981 gactttatcc tagactggac accaaatacc aaccacacag ggctttatgt tgccaaggaa
    56041 aaaggttatt tcaaagaagc tggagtggat gttgatttga aattgccacc agaagaaagt
    56101 tcttctgact tggttatcaa cggaaaggca ccatttgcag tgtatttcca agactacatg
    56161 gctaagaaat tggaaaaagg agcaggaatc actgccgttg cagctattgt tgaacacaat
    56221 acatcaggaa tcatctctcg taaatctgat aatgtaagca gtccaaaaga cttggttggt
    56281 aagaaatatg ggacatggaa tgacccaact gaacttgcta tgttgaaaac cttggtagaa
    56341 tctcaaggtg gagactttga gaaggttgaa aaagtaccaa ataacgactc aaactcaatc
    56401 acaccgattg ccaatggcgt ctttgatact gcttggattt actacggttg ggatggtatc
    56461 cttgctaaat ctcaaggtgt agatgctaac ttcatgtact tgaaagacta tgtcaaggag
    56521 tttgactact attcaccagt tatcatcgca aacaacgact atctgaaaga taacaaagaa
    56581 gaagctcgca aagtcatcca agccatcaaa aaaggctacc aatatgccat ggaacatcca
    56641 gaagaagctg cagatattct catcaagaat gcacctgaac tcaaggaaaa acgtgacttt
    56701 gtcatcgaat ctcaaaaata cttgtcaaaa gaatacgcaa gcgacaagga aaaatggggt
    56761 caatttgacg cagctcgctg gaatgctttc tataaatggg ataaagaaaa tggtatcctt
    56821 aaagaagact tgacagacaa aggcttcacc aacgaatttg tgaaataatg acagaaatta
    56881 gactagagca cgtcagttat gcctatggtc aggagaggat tttagaggat atcaacctac
    56941 aggtgacttc aggcgaagtg gtttccatcc taggcccaag tggtgttgga aagaccaccc
    57001 tctttaatct aatcgctggg attttagaag ttcagtcagg gagaattgtc cttgatggtg
    57061 aagaaaatcc caaggggcgc gtgagttata tgttgcaaaa ggatctgctc ttggagcaca
    57121 agacggtgct tggaaatatc attctgcccc tcttgattca aaaggtggat aaggcagaag
    57181 ctatttcccg agcggataaa attcttgcga ccttccagct gacagctgta agagacaagt
    57241 atcctcatga acttagcggt gggatgcgcc agcgtgtagc cttactccgg acctaccttt
    57301 ttgggcacaa gctctttctc ttagatgagg cctttagcgc cttggatgag atgacaaaga
    57361 tggaactcca cgcttggtat cttgagattc acaaacagtt gcagctaaca actctgatca
    57421 tcacgcatag tattgaggag gccctcaatc tcagcgaccg catctatatc ttgaaaaatc
    57481 gccctgggca gattgtttca gaaattaaac tagattggtc tgaagatgag gacaaggaag
    57541 tccaaaagat tgcctacaaa cgtcaaatct tggcagaatt aggcttagat aagtagaaaa
    57601 atagggagtt ggtgaagatt atcctttacc agcgcccttt ttcttttaaa aatgagaaaa
    57661 tttcggtata atagtcaaac aaggtcaagg tttaaagaga gaggtgggtt tgttatgaga
    57721 tttaaaaata catcggatca tattgaggcc tacatcaagg cgattttaga tcaatctggt
    57781 atcgtggagt tgcaacggag tcagttggca gatacctttc aggttgttcc tagtcagatt
    57841 aactacgtga tcaagacacg ctttacggaa agtagaggct acttggttga aagtaagcgt
    57901 ggtggcggag gctacattcg tataggacgg attgagtttt ctagtcatca tgaaatgctc
    57961 cgggatctgc tttactcgat tggtgagcga gtcagtcaag aaatttatga ggatattctc
    58021 cagcttttgg ttgagcagga attgatgacc aagcaggaga tgaatttgct agaatcagta
    58081 gctttggatc gcgttttagg agaagaagct ccagttgttc gagcaaacat gctacgtcag
    58141 gtcatacaag aggtagatag aaaagggaag taagatgaac tattcaaaag cattgaatga
    58201 atgtatcgaa agtgcctaca tggttgctgg acattttgga gctcgttatc tagagtcgtg
    58261 gcacttgttg attgccatgt ctaatcacag ttatagtgta gcaggggcaa ctttaaatga
    58321 ttatccgtat gagatggacc gtttagaaga ggtggctttg gaactgactg aaacggacta
    58381 tagccaggat gaaaccttta cggaattgcc gttctcccgt cgtttgcagg ttctttttga
    58441 tgaagcagag tatgtagcgt cagtggtcca tgctaaggta ctagggacag agcacgtcct
    58501 ctatgcgatt ttgcatgatg gcaatgcctt ggcgactcgt atcttggaga gggctggttt
    58561 ttcttatgaa gacaagaaag atcaggtcaa gattgctgcc cttcgtcgaa atttagaaga
    58621 acgtgcaggc tggactcgtg aagatctcaa ggctttacgc caacgccatc gtacagtagc
    58681 tgacaagcaa aattctatgg ccaatatgat gggcatgccg cagactccta gtggtggtct
    58741 cgaggactat acgcatgatt tgacagagca agcgcgttct ggcaagttag aaccagtcat
    58801 cggtcgggac aaggaaatct cgcgtatgat tcaaatattg agccgtaaga ctaagaacaa
    58861 ccctgtcttg gttggggatg ctggtgtcgg gaaaacagct ctggcccttg gtcttgccca
    58921 gcgtattgct agtggtgacg tgcctgcgga aatggctaag atgcgcgtgt tagaacttga
    58981 tttgatgaat gtcgttgcag ggacacgctt ccgtggtgac tttgaagaac gcatgaataa
    59041 tatcatcaag gatattgaag aagatggcca agtcatcctc tttatcgatg aactccacac
    59101 catcatgggt tctggtagcg ggattgattc gactctggat gcggccaata tcttgaaacc
    59161 agccttggcg cgtggaactt tgagaacggt tggtgccacc actcaggaag aatatcaaaa
    59221 acatatcgaa aaagatgcgg ctctttctcg tcgtttcgct aaagtgacga ttgaagaacc
    59281 aagtgtggca gatagtatga ctattttaca aggtttgaag gcgacttatg agaaacatca
    59341 ccgtgtacaa atcacagatg aagcggttga aacagcggtt aagatggctc atcgttattt
    59401 aaccagtcgt cacttgccag actctgctat cgacctcttg gacgaagcag cagcaacagt
    59461 gcaaaataag gcaaagtatg taaaagcaga cgattcagat ttgagtccag ctgacaaggc
    59521 cctgatggat ggcaagtgga aacaggcagc ccagctaatc gcaaaagaag aggaagtacc
    59581 tgtctacaaa gacttggtga cagagtctga tattttgacc accttgagtc gcttgtcagg
    59641 aatcccagtt caaaaactga ctcagactga cgctaagaaa tatctaaatc ttgaagcaga
    59701 actccataaa cgggttatcg gtcaagatca agctgtttca agtattagtc gtgcgattcg
    59761 ccgtaatcag tcaggaattc gcagtcacaa gcgtccgatc ggttccttta tgttcctagg
    59821 acccacaggt gtcggtaaga ccgagttggc caaggctttg gcagaagttc tctttgatga
    59881 cgaatcagcc cttatccgct ttgatatgag tgagtatatg gagaaattcg cagccagccg
    59941 tctaaatgga gctcctccag gctatgtggg ttacgaagaa ggtggggagt tgacagagaa
    60001 ggttcgcaat aaaccctatt ccgttctcct ctttgatgag gtagagaagg ctcacccaga
    60061 tatctttaat gttctcttgc aggttctgga tgacggtgtc ttgacagata gcaagggacg
    60121 caaggtcgat ttttcaaata ccattatcat tatgacatcg aacctaggtg cgactgccct
    60181 tcgtgatgat aagactgttg gttttggggc taaggatatt cgttttgacc aggaaaatat
    60241 ggaaaaacgc atgtttgaag aactgaaaaa agcttataga ccggaattca tcaaccgtat
    60301 tgatgagaag gtggtcttcc atagcctatc tagtgaccat atgcaggaag tggtgaagat
    60361 tatggtcaag cctttagtgg caagtttggc tgaaaaaggc attgacttga aattacaagc
    60421 ttcagctctg aaattgttag caaatcaagg atatgaccca gagatgggag ctcgcccact
    60481 tcgcagaacc ctgcaaacag aagtggagga caagttggca gaacttcttc tcaagggaga
    60541 tttagtggca ggcagcacac ttaagattgg tgtcaaagca ggccagttaa aatttgatat
    60601 tgcataaaag aataaaagta tcagcatctg accataagtc acagtggagt gaaactcaat
    60661 gaaaatcaaa gaacaaacta ggcagctagc cgcaggttgc tcaaaacact gttttgaggt
    60721 tgtagataga gctgacgtgg tttggagaga ttttcgaaga gtataagctg caagatgaat
    60781 gattttcttg tattgacgtt gttgttgaca aaaagtagcg gataaatgaa atccattcca
    60841 ttatcataga tgataggctg gtaggaaatt ttcaaatagc atacaggaaa tagatgtatg
    60901 gagttctggt agtagaaagg gagagagatg aacattttag ttgcagatga cgaggaaatg
    60961 attagagaag gaattgcagc atttctgaca gaagagggtt atcatgtcat tatggctaag
    61021 gatggacaag aggtcttgga aaaatttcaa gatctcccta tccatctcat ggtattggat
    61081 ttaatgatgc ctaggaagag tggttttgaa gtgttaaaag aaatcaatca aaagcacgat
    61141 attcctgtca tcgtcttgag tgctctggga gatgaaacta ctcagtcaca ggtatttgat
    61201 ctctatgctg atgatcatgt gacaaaacct ttttctttgg tactgcttgt caagcgtatt
    61261 aaggcgctta tcagacgtta ctacgtcata gaggatattt ggcgatatca ggatgtaaca
    61321 gtggatttta cctcttacaa agcacattat aaaaatgaag aaattgatct caaaccaaag
    61381 gaattactgg tactaaagtg tttgattcag cataaaaatc aagttttaag tagagagcag
    61441 atattggaag aaatttcaaa agatgtagct gatttacctt gtgatagggt cgttgatgtc
    61501 tatattcgta ctcttcgcaa aaaattagct ttagattgta tcgtgactgt gaaaaatgtt
    61561 gggtataaga ttagcttatg ataaaaaatc ctaaattatt aaccaagtct tttttaagaa
    61621 gttttgcaat tctaggtggt gttggtctag tcattcatat agctatttat ttgacctttc
    61681 ctttttatta tattcaactg gagggggaaa agtttaatga gagcgcaaga gtgtttacgg
    61741 agtatttaaa gactaagaca tctgatgaaa ttccaagctt actccagtct tattcaaagt
    61801 ccttgaccat atctgctcac cttaaaagag atattgtaga taagcggctc cctcttgtgc
    61861 atgacttgga tattaaagat ggaaagctat caaattatat cgtgatgtta gatatgtctg
    61921 ttagtacagc agatggtaaa caggtaacag tgcaatttgt tcacggggtg gatgtctaca
    61981 aagaagcaaa gaatattttg cttttgtatc tcccatatac atttttggtt acaattgctt
    62041 tttcctttgt tttttcttat ttttatacta aacgcttgct caatcctctt ttttacattt
    62101 cagaagtgac tagtaaaatg caagatttgg atgacaatat tcgttttgat gaaagtagga
    62161 aagatgaagt tggtgaagtt ggaaaacaga ttaatggtat gtatgagcac ttgttgaagg
    62221 ttattcatga gttggaaagt cgtaatgagc aaattgtaaa attgcaaaat caaaaggttt
    62281 cctttgtccg cggagcatca catgagttga aaaccccttt agccagtctt agaattatcc
    62341 tagagaatat gcagcataat attggagatt acaaagatca tccaaaatat attgcaaaga
    62401 gtataaataa gattgaccag atgagccact tattggaaga agtactggag tcttctaaat
    62461 tccaagagtg gacagagtgt cgtgagacct tgactgttaa gccagtttta gtagatattt
    62521 tatcacgtta tcaagaatta gctcattcaa taggtgttac aattgaaaat caattgacag
    62581 atgctaccag ggtcgtcatg agtcttaggg cattggataa ggttttgaca aacctgatta
    62641 gtaatgcaat taaatattca gataaaaatg ggcgcgtaat catatccgag caagatggct
    62701 atctctctat caagaataca tgtgcgcctc taagtgacca agaactagaa catttatttg
    62761 atatattcta tcattctcaa atcgtgacag ataaggatga aagttccggt ttgggtcttt
    62821 acattgtgaa taatatttta gaaagctatc aaatggatta tagttttctc ccttatgaac
    62881 acggtatgga atttaagatt agcttgtaga cagattagtt ttttattaaa gttcatatag
    62941 ggttaacata agtgtgttat tctttgtgta gataaaagaa aggatactaa tatggtatta
    63001 gcgattattt tagtaacatt ctttattcga ttgatttttt taaagcgttc gatagagaat
    63061 gagaaacgaa tccttagcaa tggcgggaaa gaatttggag ttgagaatac aaaacgatta
    63121 actatggctc atattgtttt ttatctctct tgcttggttg aggcaatggt gcacaagaca
    63181 atttttgatg gcatgggcat ggttggttta gtcttgctta ttttttctat gctgatgttg
    63241 atgttggtga ttcacttgtt gggagatatt tggacagtga agcttatgct tgtcaataat
    63301 cacaaatatg tagatcatat cttgtttagg acagtaaaac atcctaatta ctttttaaat
    63361 attcttcctg agttgattgg cttgaccttg ttgagtcatg cttatgtgac ttttgtttta
    63421 gtttttccag tttatgcagt tattttgtat cgacgaatag ctgaagagga aaagctatta
    63481 catgaagtta taatcccaaa tggaagcata aagagataaa tacaaaattc gatttatata
    63541 cagttcatat tgaagtgata tagtaaggtt aaagaaaaaa tatagaagga aataattatg
    63601 tttgcatcaa aaagcgaaag aaaagtacat tattcaattc gtaaatttag tgttggagta
    63661 gctagtgtag tagttgctag cttgttctta ggaggagtag tccatgcaga aggggttaga
    63721 agtggggata cccccaaggt tacatctagt gggcaagata tatcgaagaa gtatgctgat
    63781 gaagtcaagt cgcatctaga aaaaatattg agtgagatcc aattagataa aagaaaacat
    63841 acccaaaatc tcgccttcaa caaaaagttg agcagaattc aaacggagta tttctattta
    63901 aaaaagaagt tgaaagctga gttgacgtca aaaacaaaag aagagttaac gtcaaaaaca
    63961 gaagagttga cgtcaaaaac aaaaaaagag ttagacgcag cttttgagca gtttaaaaaa
    64021 gatacattat caacagaacc agaaaaaaag gttgcagaag ctcagaagaa ggtagcagaa
    64081 gctaagaaaa aagccgagga tcaaaaagaa gaagatcgtc gtaactaccc aaccaatact
    64141 tacaaaacgc ttgaacttga aattgctgag ttcgatgtga aagttaaaga agcggagctt
    64201 gaactattaa aagagaaagc taaggaatct cgagacgagg aaaaaattaa gcaagcagaa
    64261 gcggaagttg agagtaaaca agctgaggct acaaggttag aaaacatcaa gacagatcgt
    64321 aaaaaagcag aagaagaagc taaacgaaaa gcagatgcta agttgaagga agctaatgta
    64381 gcgacttcag atcaaggtaa accaaagggg cgggcaaaac gaggagttcc tggagagcta
    64441 gcaacacctg ataaaaaaga aaatgatgcg aagtcttcag attctagcgt aggtgaagaa
    64501 actcttccaa gctcatccct gaaatcagga aaaaaggtag cagaagctga gaagaaggtt
    64561 gaagaagctg agaaaaaagc caaggatcaa aaagaagaag atcgccgtaa ctacccaacc
    64621 aatacttaca aaacgcttga acttgaaatt gctgagtccg atgtgaaagt taaagaagcg
    64681 gagcttgaac tagtaaaaga ggaagctaag gaacctcgag acgaggaaaa aattaagcaa
    64741 gcaaaagcgg aagttgagag taaacaagct gaggctacaa ggttagaaaa aatcaagaca
    64801 gatcgtaaaa aagcagaaga agaagctaaa cgaaaagcag cagaagaaga taaagttaaa
    64861 gaaaaaccag ctgaacaacc acaaccagcg ccggctcctc aaccagaaaa accagctcca
    64921 aaaccagaaa aaccagctcc agctccaaaa ccagagaatc cagctgaaca accaaaagca
    64981 gaaaaaccag ctgatcaaca agctgaagaa gactatgctc gtagatcaga agaagaatat
    65041 aatcgcttga ctcaacagca accgccaaaa actgaaaaac cagcacaacc atctactcca
    65101 aaaacaggct ggaaacaaga aaacggtatg tggtacttct acaatactga tggttcaatg
    65161 gcgacaggat ggctccaaaa caatggctca tggtactacc tcaacagcaa tggcgctatg
    65221 gcgacaggat ggctccaaaa caatggttca tggtactatc taaacgctaa tggttcaatg
    65281 gcaacaggat ggctccaaaa caatggttca tggtactacc tcaacgctaa tggttcaatg
    65341 gcgacaggat ggctccaata caatggttca tggtactacc tcaacgctaa tggtgatatg
    65401 gcgacaggat ggctccaaaa caatggctca tggtactact taaacgctaa tggtgatatg
    65461 gcgacaggat ggctccaaaa caacggttca tggtactacc taaacagcaa tggtgctatg
    65521 gtaacaggat ggctccaaaa caatggctca tggtattacc tcaacgctaa tggtgatatg
    65581 gcgacaggtt gggtgaaaga tggagacacc tggtactatc ttgaagcatc aggtgctatg
    65641 aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg ctcaggtgcc
    65701 cttgcagtca acacaactgt agatggctat ggagtcaatg ccaatggtga atgggtaaac
    65761 taaacctaat ataactagtt aatactgact tcctgtaaga actttttaaa gtattcccta
    65821 caaataccat atcctttcag tagataatat acccttgtag gaagtttaga ttaaaaaata
    65881 actctgtaat ctctagccgg atttatagcg ctagagacta cggagttttt ttgatgagga
    65941 aagaatggcg gcattcaaga gactctttaa gagagttacg ggttttaaac tattaagcct
    66001 tctccaattg caagagggct tcaatctctg ctagggtgct agcttgcgaa atggctccac
    66061 ggagtttggc agcgccagat gttccacgga gatagtgagg agcgaggccg cggaattcac
    66121 gaactgcgac gttttctcct ttgaggttaa tcaatcgttt caagtgttcg taggcgatct
    66181 tcatcttgtc ttcaaaggtc aaatcaggta ggatttctcc tgtttcaaag taatggttga
    66241 tttggttgaa gaggtaagga tttcccatgg cagctcggcc aatcatgact gcgtcagcac
    66301 caacttcttc gatgcgttgc ttggcttctt ggacagtacg gatatcaccg ttggcgatga
    66361 atggaatctt ggttagagct tgggcaacct tgtaaagggt ctcaaggtct gcgtggccag
    66421 tatacatttg ttcacgggta cggccatgca tggcgagggc agaaacacct gcagcttcag
    66481 cagcgagggc attttctact gccagagatg ggtccgccca gccggtacgc attttgacag
    66541 taagtgggat atcaaggaca gactggacct tgttgatgat ggagtaaatc ttgtctggat
    66601 ccttgagcca catagcacca gcttcgttct tcacgatttt gttgacaggg cagcccatgt
    66661 tgatatcgac gatatcggtc ttggtgtttt cttggatgaa ttctgctgcg cgtgctaggc
    66721 tgtcttcatc gctaccaaaa agttggatag agacagggtt ttcgccctca tcgatatgaa
    66781 gcatgtgcag ggttttttcg ttgttgtatt ggattccctt gtcagagacc atttccatta
    66841 caacgagtcc agctccgagc tcttttgcga tggtacgaaa ggctgagttg gtcacgccag
    66901 ccataggcgc taaaacggta cgattgggaa tctcaacatt gccaatcata aaaggtgtat
    66961 taagatttgt cacgaatgag ttcctccagg tccttttcat caaagttgta agtagtttgg
    67021 cagaattgac aagtgatttc tgccccgtgg tcttcttctt tcatttcctg taagtctgag
    67081 cttggaaggc tggcaagagc gttcataaag cgttcatggc tacagtcaca ttggaaacgg
    67141 atttcttctt cagaaagacg cttgtaggct tcgtccccgt agatagcctt gaggagggct
    67201 tcgatatggt cgtcgctttc gagaagagta gagatagctg gcatttcttg gatgcgtttt
    67261 tcaaagcgag caatctcttc tttcttggct cctggcaaga cttgaactag gaaaccacct
    67321 gcaaccttga ccttgtcttc ctcgtccaaa aggacattga ggccgaccgc tgaaggcgtt
    67381 tgttggcttt cagtaaggta aaaggcaagg tcttcaccga tttctccaga gatgagggga
    67441 gttatagagt tgtaaggatt tccagtaccg tagtctgtga taacgaggaa ttgaccattt
    67501 ccaacaaaag gtccgactag gacttcacca gtcgcagtct ttttgatgtc aacaccagga
    67561 ttttgaacgt agcctttgac gttccccttg gtatcagcga cggtgataat agcacctaga
    67621 gagctagatc ccaacacctt aactgtaagt ttggtatttc ctttttcatt ggctgcgaga
    67681 atctggctgg cgataagagt tcgaccaagc gctacagttg agctagcttg ggtttgatgt
    67741 ttttcttgag cagtgcggac ggtttcagtg ctatcaagga caaaagcacg aaaggctccg
    67801 ctttctgata tagttttaat aattttatcc atagctacta ttttagcata aaaatgccca
    67861 aagggggagc cgtgtgttta ctgattttca ggataatgga ccaggaaatc agcatgaaaa
    67921 taaaaagaga aacagattat tttagcattt gtaagattta tgctatgctt aaggtagaaa
    67981 atgaaagggg taacaaatgt atttaggaga tttgatggag aaagccgagt gtggtcaatt
    68041 ttcaatcctt tcctttctat tacaagagtc tcagacgacc gtcaaggctg taatggaaga
    68101 aacaggattt tcaaaagcaa ccctaaccaa atatgtcacc ctgctcaatg acaaggcttt
    68161 ggatagtggc ttagagctgg ctattcactc agaagatgaa aatctgcgtc tgtctatcgg
    68221 tgcagctacc aaggggaaag atattcggag cttgtttttg gagagtgctg ttaaatacca
    68281 gattttggtt tatcttctct accaccaaca gtttttagcc catcagctgg ctcaagaatt
    68341 ggtgattagc gaggctacgc ttggtcgtca cttggctggt ttaaatcaga ttttgtcaga
    68401 atttgattta tccatccaaa atggacgttg gcgaggtcca gagcatcaga ttcgctattt
    68461 ctatttctgt cttttccgaa aggtctggtc gagtcaggaa tgggaaggtc acatgcagaa
    68521 accagagaga aaacaggaga ttgccaattt agaggaaatc tgcggtgcaa gtttgtctgt
    68581 ggggcagaaa ttggacttgg ttctctgggc tcacatcagt caacaacgtc ttcgggtcaa
    68641 tgcttgtcag tttcaagtca tagaagagaa aatgcgaggg tattttgaca atatcttcta
    68701 tcttcgtttg ctgagaaagg ttccgtcctt ttttgctggg caacatattc cactaggagt
    68761 tgaggatggt gagatgatga tattcttctc ttttctccta tctcatcgca ttcttcctct
    68821 tcatactatg gaatatattc ttggttttgg agggcagttg gcagatttac tgacgcaatt
    68881 gattcaagaa atgaagaagg aggaactatt gggggattat acagaggacc atgtcaccta
    68941 tgaactcagt cagctttgtg ctcaagtcta tctctataag ggctatattt tacaggatcg
    69001 ctacaagtac cagttagaga atcgtcatcc atatttactg atggaacatg attttaaaga
    69061 gacagcagag gagatttttc atgctctacc tgcttttcaa caggggacag atttagataa
    69121 gaagattctc tgggaatggc tccagttaat cgaatatatg gctgaaaacg gtggccagca
    69181 tatgcggatt ggtctggatt tgacatctgg ttttcttgtc ttttcaagga tggcagccat
    69241 tttgaaacgg tatttggaat acaatcgttt tattaccatt gaagcttatg accctagtcg
    69301 gcattatgat ttgctggtta ccaataaccc gattcataag aaggaacaga ccccagtcta
    69361 ttatttaaaa aatgacttgg atatggaaga tttggcaggg attcgtcagt tattattcac
    69421 ttaaaaggct tggttaatcc aggtcttttt tgtgaaattc acacaatctc ctcacatttt
    69481 tttaaaaatt aaaaaaagtt gataaacaag aaagcgcttt attttgtata ctagtaagtg
    69541 taaagaggaa acacctcaag atctttatca ggaggacagt acatgtcaca agaaaaatac
    69601 atcatggcta ttgaccaggg aactacaagt tctcgtgcca tcattttcaa caaaaaaggg
    69661 gaaaaggtta gctcgagtca aaaagagttt acccagattt tccctcaggc aggttgggtt
    69721 gagcacaatg ccaatgaaat ttggaactct gttcagtcag ttattgcggg tgctttcatt
    69781 gaaagtggtg tcaagccaaa tcaaatcgag gcaatcggga ttaccaacca acgtgaaaca
    69841 acggttgtct gggataagaa aacaggactt cctatctaca atgctatcgt ttggcagtca
    69901 cgccagacag cacctttggc tgagcaacta aaaagccaag gttatgtgga aaaattccat
    69961 gaaaagactg gtttgattat tgatgcttac ttctctgcta ccaaggttcg ttggattttg
    70021 gatcatgtag aaggtgctca agagcgagca gaaaaagggg aattgctctt tggtactatc
    70081 gatacttggt tggtttggaa attgactgac ggtgcggctc acgtgactga ctactcaaat
    70141 gcagctcgta ccatgcttta taacattaaa gaactcaaat gggatgatga gattttggaa
    70201 atccttaaca ttccgaaggc tatacttcca gaagttcgtt ctaactccga aatctacggc
    70261 aagacagctc cattccattt ctacggtgga gaggtgccaa tctcaggtat ggctggggac
    70321 caacaagcag ccctctttgg acagttggct tttgagccag gtatggttaa gaatacttat
    70381 ggaacaggct ctttcatcat catgaatact ggggaagaga tgcagttgtc tgaaaacaac
    70441 ctcttgacaa ccattggtta cggaatcaac ggtaaggttt attatgcctt ggaaggttct
    70501 atcttcatcg caggaagtgc tattcagtgg cttcgtgacg gtcttcgcat ggttgaaaat
    70561 tcaccagaat ctgaaaaata cgctcgtgat tctcacaaca acgatgaagt ttatgtcgtt
    70621 ccagccttta caggtctagg cgctccatac tggaaccaaa atgctcgtgg ttccgtcttt
    70681 ggtttgactc gtggaacaag caaagaagac tttatcaagg cgactttgca atctattgct
    70741 tatcaagtgc gtgatatcat cgacaccatg caagtggata ctcagaccgc cattcaagta
    70801 ctgaaggtgg atggtggtgc agccatgaac aacttcctca tgcagttcca ggcggatatt
    70861 ttaggcattg acattgcacg tgctaaaaac ttggaaacaa cagctctagg agcggccttc
    70921 ctagcaggtt tgtcagtagg gtactggaaa gacttggacg agttgaaact cttgaacgag
    70981 acaggagaac tctttgagcc atctatgaac gaatctcgca aggaacaact ctacaagggc
    71041 tggaagaagg ctgtgaaagc aactcaagtc tttgcggaaa tcgatgacta atactcaatg
    71101 aaaatcaaag agcaaactag gaagctagcc gcaagctgta cttgagtacg gtaaggcgac
    71161 gctgacgtgg tttgaagaga ttttcgaaga gtataatact ggcagaataa agcgatttag
    71221 ttagaaagtg tgtaaatatg gaattttcaa agaaaacacg tgaattgtca attaaaaaaa
    71281 tgcaggaacg caccctggac ctcttgatta tcggtggagg aatcacagga gctggtgtag
    71341 ccttgcaggc ggcagctagc ggtcttgaga ctggtttgat tgaaatgcaa gactttgcag
    71401 aaggaacatc tagtcgttca acaaaattgg ttcacggagg acttcgttac ctcaaacaat
    71461 ttgacgtaga agtggtctca gatacggttt ctgaacgtgc agtggttcaa caaatcgctc
    71521 cacacattcc aaaaccagat ccaatgctct taccagttta cgatgaagat ggagcaacct
    71581 ttagcctctt ccgtcttaaa gtagccatgg acttgtacga cctcttggca ggtgtgagca
    71641 acacaccaac tgcgaacaag gttttgagta aggatcaagt cttggaacgc cagccaaact
    71701 tgaagaagga aggcttggta ggaggtggag tgtatcttga cttccgtaac aacgatgcgc
    71761 gtctcgtgat tgaaaacatc aaacgtgcca accaagacgg tgccctcatt gccaaccacg
    71821 tgaaggcaga aggcttcctc tttgacgaaa gtggcaagat tacaggtgtt gtagctcgtg
    71881 atctcttgac agaccaagta tttgaaatca aggcccgtct ggttattaat acaacaggtc
    71941 cttggagtga taaagtacgt aatttgtcta ataagggaac gcaattctca caaatgcgcc
    72001 caactaaggg agttcacttg gtagtggatt caagcaaaat caaggtttca cagccagttt
    72061 actttgacac aggtttgggt gacggtcgta tggtctttgt tctcccacgt gaaaacaaga
    72121 cttactttgg tacaactgat acagactaca caggtgattt ggagcatcca aaagtaactc
    72181 aagaagatgt agattatcta cttggcattg tcaacaaccg ctttccagaa tccaacatca
    72241 ccattgatga tatcgaaagc agctgggcag gtcttcgtcc attgattgca gggaacagtg
    72301 cctctgacta taatggtgga aataacggta ccatcagtga tgaaagcttt gacaacttga
    72361 ttgcgactgt tgaatcttat ctctccaaag aaaaaacacg tgaagatgtt gagtctgctg
    72421 tcagcaagct tgaaagtagc acatctgaga aatatttgga tccatctgca gtttctcgtg
    72481 ggtctagctt ggaccgtgat gacaatggtc ttttgactct tgctggtggt aaaatcacag
    72541 actaccgtaa gatggctgaa ggagctatgg agcgcgtggt tgacatcctc aaagcagaat
    72601 ttgaccgtag ctttaaattg atcaattcta aaacttaccc tgtttcaggt ggagaattga
    72661 acccagcaaa tgtggattca gaaatcgaag cctttgcgca acttggagtt tcacgtggtt
    72721 tggatagcaa ggaagctcat tacctagcaa atctttacgg ttcaaatgca ccgaaggtct
    72781 ttgcacttgc tcacagcttg gaacaagcgc caggtcttag cttggtagat actttgtccc
    72841 ttcactatgc aatgcgcaac gagttggctc ttagcccagt tgacttcctt cttcgtcgta
    72901 ccaaccatat gctctttatg cgtgatagct tggatagcat cgttgagcca gttttggatg
    72961 aaatgggacg attctatgac tggacagaag aagaaaaagc aacttaccgt gctgatgtcg
    73021 aagcagctct cgctaacaac gatttagcag aattaaaaaa ttaagaaaaa ataaaagagg
    73081 tggagggcag cattccttgt cgcccgtccc ttctttttaa tggagacaga aagatgatga
    73141 atgaattatt tggagaattt ctagggactt taatcctgat tcttctagga aatggtgttg
    73201 ttgcaggtgt ggttcttcct aaaaccaaga gcaatagctc aggttggatt gtgattacta
    73261 tgggttgggg gattgcagtt gcggttgcag tctttgtatc tggcaagctc agtccagctc
    73321 atttaaaccc agctgtgacc atcggtgtgg ccttaaaagg tggtttgcct tgggcttccg
    73381 ttttgcctta tatcttagcc cagttcgcag gggccatgct gggtcagatt ttggtttggt
    73441 tgcaattcaa acctcactat gaggcagaag aaaatgcagg caatatcctg gcaaccttca
    73501 gtactggacc agccatcaag gatactgtat caaacttgat tagcgaaatc cttggaacct
    73561 ttgttttggt gttgacaatc tttgctttgg gtctttacga ttttcaggca ggtatcggaa
    73621 cctttgcagt gggaactttg attgtcggta tcggtctatc actaggtggg acaacaggtt
    73681 atgccttgaa cccagctcgt gaccttggac ctcgtatcat gcacagcatc ttgccaattc
    73741 caaacaaggg agacggagac tggtcttacg cttggattcc tgttgtaggc cctgttatcg
    73801 gagcagcctt ggcagtgctt gtattctcac ttttctagtt tatactcttc gaaaatcaaa
    73861 ttcaaaccac gtcagcgtcg ccttaccgta ctcaagtaca gcttgcggct agcttcctag
    73921 tttgctcttt gattttcatt gagtattaga aaacaattat gttgatagag cttgggcaag
    73981 agcccaattt cagcaaaaaa tgaagtaaat cttctcataa taaaacgcat catatcaagc
    74041 acgaaaattc cacgaggtca actacagtca gaaagctgaa caacaagcca aaacgcccaa
    74101 aaaaggcggc aaaaagcaag cacctgcaag caacgtgccg aaatggtcaa atcctgatta
    74161 tgtcaacgaa ttagacccaa aaatcgttga tatgctagta gaatttcaca agtcacaagg
    74221 cactttggaa actcccgagg cgcaagcaga aatcgcccaa aaacgtgaag aaatcgagca
    74281 aaggagagct gagcttgagg ataaaaaaca agagcttttg aaccgcttga acaaatagag
    74341 tttcgcaagt attatgctta caaattactt gagcaattaa ctaaaatata aaccctgcct
    74401 ttatatctag gcagggttta tattttagaa attcacgtag gttgttacgg tttttacata
    74461 cccagtatag tttgagtttc tatagtattc agtgataaac ttccattttc tttgagcaac
    74521 atggatataa gtacttgtta tgtagtatgg atatgggctt tgtgaatcca agtaagactg
    74581 ataagcttgt ataccaaaat atgctccacc aattattgca ccccatggac cccccaataa
    74641 agcacctatc ctaccaatca tataactgat tccagcacca gtcatgaagt tagcgaatgt
    74701 gttagcttgt ttattcccat gtattgtgtt gacgtaattc caaacattag gatcgtatga
    74761 tctaaaagat atatttaggt cgatttcatt cttttgataa gccatataaa atgccccatt
    74821 gatatagacg ccgtcagcac gtcgttcaat agtgtctaca cttccatctg gattgacaac
    74881 ctcaagaact tcatcgctta aaatatttac ttgcgtatct ccgaaccgca ctgatgagcc
    74941 attctcaaac tgagcctcac cagatacaac tttagagttt gccgataagc tatcatcagc
    75001 aaaaacaaca agcgacgggg aaatgctaga catacagaaa acagacataa ctagcaaaca
    75061 catgcattta aacatcttag acataacgga aactcctttg tatttttgat ttttttcaac
    75121 ttttattata caataaaacc aaataaaaag aaagcggtaa caatatgctt aatgcgaaaa
    75181 ttttttatat atttttatgt ttgatcgtta tcgaaactac aggcttgttg ttgttgaaaa
    75241 gaggtctcga aatgggttat ttagacacag aagctattat cctcgcagtt ttttcatttg
    75301 ctttttacaa cctatgttca ttcgcttggg tctgctctac aataaaaaac aataaaaaat
    75361 aaatagacgt attttcaaaa aaaaaaaaat gcatatttat cttagcaaaa cgacgattta
    75421 aatcgtcgtt tttttgtagt acgacgggca tgtcgtatat ctgaggtgta agtcctcggt
    75481 gggcacctgc taccggtgaa cccaatagcg attcccaagc ctgactatcg tgaggtagca
    75541 gggagaggaa gggatagcga aatcgtggct ctacgaacag gaacgtgata gtaaggcgta
    75601 tatagcggat aaggaggctt caaactctaa agtccaaaaa ggtagtcgta acctatatgt
    75661 gtaaatcacg agagtaattg aattcggact aaggtttgtg tgaaaaagat aaatctttct
    75721 agagtctaaa gactctgcgt cagatttcct attttcactg taacctttta acgtcctcat
    75781 atcttgtata aacgaggaaa gatgtacgac ttatcccgtg aggtttcatg agcgctgaaa
    75841 gcgtagtaac aacgaatcat gagaagtcag ccgagcccat agtagtgagg aaacttccgt
    75901 aatggaagtg gagcgaaggg gtgaacaact aatcgaagta atcctacttc acttgtgtct
    75961 gtaaaatgag tggtctgata gaactggact ttgacacgta atgactagat gaaggttcac
    76021 caatataaga tgtccctcag gcaccaaaac aagaaaggaa tacgcacatg tcaaaactgc
    76081 tagataagat attatcacgc gaaaatatgc tggaagccta caatcaagta aaatccaata
    76141 aaggctcagc tgggattgat ggaatgacta tcgaagagat ggataattat ctcagacaaa
    76201 actggcgctt gactaaggaa ctgataaaac agagaaaata taagcctcaa ccagttctta
    76261 aagttgagat acctaaacca gacggaggca tccgtcaact aggaattcca acagttatgg
    76321 atagaatgat tcaacaggcc attgtccaag tcatgagccc catttgtgaa ccccatttct
    76381 cagatacgag ttatggtttc agaccaaata ggtcatgtga aaaagccatc atgaagctct
    76441 tagaatactt aaatgacggc tatgagtgga tagtggatat agacctagag aaatttttcg
    76501 atacagttcc tcaagataga ttgatgtcct tagtacataa cattatcgaa gacggagata
    76561 cggaatcctt gattcgtaag tatcttcatt caggtgttat cattaatggt caacgttata
    76621 aaacactagt tggtacacca cagggaggaa atttatctcc tctcttatcc aatatcatgc
    76681 ttaatgaatt ggacaaggaa ttagaaaaga ggggacttcg atttgtgcgc tacgcagatg
    76741 attgtgtgat tacggtcgga agcgaggcag cctctaagcg tgtgatgtat tcagtcagtc
    76801 gttttattga gaaacggcta ggtttgaaag taaacatgac caagactaag attaccagac
    76861 caagagagtt gaaatatcta ggttttgggt tctggaaatc atcagatggt tggaaaagcc
    76921 gtccacatca agatagtgtt cggagattta agtttaaatt gaagaaacta acacagagga
    76981 aatggagtat agacctaaca agacgtattg agcaactgaa tttgtctatt cgaggatgga
    77041 taaactattt ctcattggga aatatgaaaa gtatagtcgc cagcatagat gagcgcttgc
    77101 gtactcgcct acgagtgatt atctggaagc aatggaagaa gaaatcgaga cgattatggg
    77161 gattgcttaa gttaggagtt cctaaatgga tagcagataa ggtatctggc tggggcgacc
    77221 attatcaatt agtagctcag aagtcggtac ttaaacgtgc tatatcaaaa ccagtcctgg
    77281 aaaaacgtgg actggtttcg tgtttggatt attaccttga acgacatgcg ttaaaagtta
    77341 gttgaaccgc cgtatgccga acggcacgta cggtggtgtg agaggggcta gagattatcc
    77401 cctactcgat taactcccct gaaatttatt ttaattatgc aaatttcacg tatttttgat
    77461 gctgagacga cgatcctggg aacttttcag atattttttt gactatctaa atctatcatt
    77521 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccag
    77581 tctctttttc gattgaagat gtaattattc tactaactaa ctaacttctt agtactagcc
    77641 aacaacgata atcataattc ctcctaaaat taggaataat aaaggcaata gtttttgttt
    77701 tttcatgtaa aaaacctcac ttttgttttc tgctatttta tgctaaaata ttaaaaatca
    77761 aatttaattc caaagtttgt aactaaaggg ggagcgctac atgtctaatt catttgtcaa
    77821 gttgttagtc tctcaattat ttgcaaattt agcagatatt ttctttagag taacaatcat
    77881 tgctaacata tacattattt caaaatcagt aattgccaca tcactagttc ctatcttaat
    77941 aggaatatcc tcttttgttg cgagtctttt agttccgttg gttactaaaa ggttagcgct
    78001 aaatagggtt ttatctttat ctcaatttgg aaagactata ttattggcga tactggtagg
    78061 aatgtttacc gtaatgcaat ccgtagcgcc tttggtgacc tatctatttg ttgttgcaat
    78121 ttccatacta gatggttttg cagcacccgt ttcctatgct attgtgccac gctatgcgac
    78181 cgatttgggt aaggctaatt cagccttatc aatgactggt gaagctgttc aattgatagg
    78241 ttggggatta ggtggactct tgtttgcaac aattggtctg ttacctacca cgtttatcat
    78301 tttagtcttg tatatcattt ctagctttct gatgttattt cttcctaacg ctgaagtgga
    78361 ggtgttagag tcagaaacta atcttgaaat tttgctcaaa ggttggaagt tagttgctag
    78421 aaatcctaga ttaagacttt ttgtatcagc aaatttattg gaaatttttt caaatacgat
    78481 ttgggtttct tccattatac ttgtttttgt aacggagtta ttaaataaaa cggaaagtta
    78541 ctggggatat tctaatacag catactctat tggtattata attagtggct taattgcttt
    78601 taggctatct gaaaagttcc ttgctgctaa atgggaaagt attctttttc ctcttgtagc
    78661 tatggcaata gtgacactga ctattctata ttttccaaac gcacagatgt ttttattatt
    78721 ttcagcttta gttggtatgt tatcgcaact aaaagaagtt cctgaaagtg tatttcttca
    78781 ggaaacagta gaggaaaatc atttagttaa tgtctattcc gttcttgaag tgatttctac
    78841 attagcattt tcagtatttg ttttgctaat gagctatatt acttagagtt ttggtattag
    78901 catcagtttt tggctatcag ccatttgtct gatgatagaa gctattttaa tttatatcag
    78961 acgagattat tttaaatgag agagtggtta aaaagtcttt aaaaatcaga aaagcgcata
    79021 gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt atgggaatat ttacttcatt
    79081 gtctcctgaa atggagtttt gaagcaggat cactctattt gaaaatatac atagaaaaac
    79141 tgtataatcc ttaaaataat ctgaaatcta ttggaaacat acggtaaaca attgaaaaga
    79201 tactatataa agtggtacaa tggtagaaaa ataagctagt aagaatcact cttattttag
    79261 cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca gagaaaagaa ttgtacctct
    79321 ttcttggtcg gacagccttg tattttctta tctttctagg gctgctttac ttctttagct
    79381 atcttggtca gggtcaagga agctttatct ataatgaatt ttaacttgaa ggagaatccc
    79441 tattgtgtca aataaaccaa tagcagatat gattgaaacc attgagcatt ttgctcagac
    79501 acagcctagc tatcctgtct ataatgtttt ggggcaggaa cacacttatg gcgatttaaa
    79561 ggctgattcg gatagtttgg ctgcagtcat tgaccaacta ggcttgcctg agaagtctcc
    79621 tgtggttgtt tttggtggcc aagaatatga aatgttggca acctttgtag cgctgactaa
    79681 gtcaggtcat gcctacattc caattgatag ccattcggcc ttggagcgag tttcagctat
    79741 tttagaagta gcagagccaa gcttgattat tgccatttca gcctttccct tggagcaggt
    79801 ttttacacca atgataaatc tagctcaggt tcaagaagcc tttgcccaag ggaataacta
    79861 tgaaatcacg catccagtca agggagatga taattactac attatcttta cttctggtac
    79921 gactggtaag cctaagggag tgcagatttc acatgataat ctcctcagct ttacaaactg
    79981 gatgattacg gataaggaat ttgcgacacc gagtcgtccg caaatgctgg cacagccacc
    80041 ttattctttt gacttgtctg tcatgtattg ggcaccgacc ttggcacttg gtggtacgct
    80101 tttcactctt ccttcagtca tcactcagga ctttaagcaa ctctttgcgg ctatcttttc
    80161 attgccaatc gctatctgga catcaacacc atcctttgca gatatggcca tgttgtctga
    80221 atacttcaac agtgagaaaa tgcctggaat cacgcatttc tactttgatg gtgaagaatt
    80281 gacggtcaaa acagctcaaa aactgcgcga gcgtttccca aatgcccgta tcatcaatgc
    80341 ttacggccca acagaagcga cagtagctct gtcagcagtt gccgtgacag acgagatgtt
    80401 agcgactctc aaacgcctac caatcggcta taccaaggct gattctccaa cctttatcat
    80461 tgacgaggaa ggaaataaac tgccaaatgg tgagcaggga gaaatcattg tttctgggcc
    80521 agctgtttca aaaggttata tgaacaatcc tgaaaaaaca gcagaagcct tctttgagtt
    80581 tgaagatctg ccagcctatc acacaggcga tgtgggaacc atgacagatg agggcttgct
    80641 tctctacggc ggacgcatgg acttccagat taagtttaac ggttaccgca ttgagttaga
    80701 agatgtctct caaaacctca acaagtctcg ctttatcgaa tctgctgtcg cagtaccgcg
    80761 ctataacaag gaccacaagg tacaaaatct attggcttat gtcatcttaa aagacggtgt
    80821 tcgtgagcag tttgagcgag atatcgatat taccaaggcc atcaaggaag acctgacaga
    80881 catcatgatg tcctatatga tgccatctaa attcctttac cgagacagtt tgccactaac
    80941 tccaaatgga aagattgaca tcaaaggatt gattaacgag gtgaataaga gatgatggag
    81001 ttttttcaac agcttcctca tttagagcca tatggcaatc ctcagtattt tgtttatgtg
    81061 attgctgcaa ccttgcccat ctttataggt ctctttttca agaaacgctt tgcctggtat
    81121 gaagtgttgg taagtctctt ctttattgtc accatgttgg tgggtggaaa gaccaatcaa
    81181 ctagctgcct tgggtattta cctttgctgg gaaatattgc tcctgctttt ctacaagcat
    81241 tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag ttagttttct gtccctactt
    81301 ccgattatct ttgtcaaggt gcaaccagct atcaatggaa cgcagtcttt gcttgggttc
    81361 ttgggaattt cttacctgac ctttcgttcg gttggaattg tcatcgagct gagagatgga
    81421 gtgattaagg attttaccct ctgggaattc ctccgtttcc ttctcttcat gccaactttc
    81481 tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa attatcaggc tattcctgag
    81541 cgagatgagt tgatggatat gctggatgaa tctgtccgct atatcatgtg gggctttttg
    81601 tataagttta tcctagctca tgttttagga gagaccttac tacctcctct gaagaattta
    81661 gccttgcagt caggtggctt ctttaatctc tatgccttgg cagttatgta tacttttggt
    81721 ctggagctct tctttgactt tgcaggttat tctatgtttg ctttggccat ctcaaacttg
    81781 atgggaatcc gtagccctat caactttaac aagccctttt tatcaaggga tttaaaggag
    81841 ttttggaatc gctggcatat gagtctgtcc ttctggttcc gtgactttgt ctttatgcga
    81901 atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc gtaatgtaac ctcaagcatg
    81961 gcctacattg taaatatgct gattatggga ttttggcatg gcgtgacctg gtactatatc
    82021 gcctatggac tctttcatgg actaggcttg gtcatcaatg atgcctgggt tcgcaagaaa
    82081 aaaacgctca ataaggaacg gaaaaaagca gggaaggctg ccctacctga gaatcgctgg
    82141 attcagtttc ttggcatggt tgtcactttc catgttgtca tgttgtcatt cttaatcttt
    82201 tctggattct tgaataatct atggtttaaa aaataaaagg aaataaaata tggatatcaa
    82261 atcagaagtt attgaaatta ttgatgagtt gtttatggaa gatgtttctg acatgatgga
    82321 tgaagatctt tttgatgcag gtgtcttgga tagtatggga acggttgagt tgattgtgga
    82381 gattgagaac cgttttgaca ttcgtgtccc tgtaacagag tttggtcgtg acgactggaa
    82441 tacagctaat aaaatcatag ctggtattgt ggagctacaa aatgcttaaa cgcttatgga
    82501 tgatcttcgg accggtcttg atcgctggtt tgttggtttt tctgctcatt ttcttttatc
    82561 ctactgagat gcatcataat ctaggagctg aaaagcgttc agcagtggct actactatcg
    82621 atagttttaa ggagcgaagt caaaaagtca gagcactatc tgatccaaat gtgcgttttg
    82681 ttcccttctt tggctctagt gaatggcttc gttttgacgg tgctcatcct gcggtattag
    82741 ctgagaaata caatcgttcc taccgtcctt atcttttagg acagggggga gctgcatcgc
    82801 ttaaccaata ttttggaatg caacagatgt taccacagct ggagaataaa caagttgtgt
    82861 atgttatctc acctcagtgg ttcagtaaaa atggctatga tccagcagcc ttccagcagt
    82921 attttaatgg agaccagttg actagttttc tgaaacatca atctggggat caggctagtc
    82981 aatatgcagc gactcgctta ctgcaacagt tcccaaacgt agctatgaag gacctggttc
    83041 agaagttggc aagtaaagaa gaattgtcga cagcagacaa tgaaatgatt gaattattgg
    83101 ctcgttttaa tgaacgccag gcctcctttt ttggtcagtt ttcggttaga ggctatgtta
    83161 actacgataa gcatgtagct aagtatttaa aaatcttgcc agaccagttt tcttatcaag
    83221 caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac ttccaataat gagatgggaa
    83281 tggaaaatta tttctataat gagcagatca agaaggattt gaagaaatta aaggattctc
    83341 agaaaagctt tacctatctc aagtcgccag agtataatga cttgcagttg gttttaacac
    83401 agttttctaa atctaaggta aacccgattt ttatcattcc acctgttaat aaaaaatgga
    83461 tggactatgc tggtctacga gaggatatgt accaacaaac ggtgcagaag attcgctacc
    83521 agttagaaag tcaaggtttt accaatatag cagatttttc taaggacggc ggggagcctt
    83581 tctttatgaa agacaccatt caccttggtt ggttgggttg gttggctttt gacaaggcag
    83641 ttgatccttt cctatccaat cccacaccag ctccgactta ccatctgaat gagcgctttt
    83701 tcagcaaaga ttgggcgact tatgatggag atgtcaaaga atttcaatag ataatataat
    83761 atagaagagt tcaagaatga aactagtttt cacgtttttt cttgactctt tttttgcttg
    83821 tgatataatt aactggtaaa caaaatgtca aagaatagta tttttctctt aaaaaagaga
    83881 agtctaaaag gaaaggagtc agatatgaga cagctagcaa aggatatcaa tgcttttttg
    83941 aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc taataggtca ttgcactagc
    84001 gaggtggccc tgaccaatac tcaggagcat atccttatgc tcttgtcaga ggaatcttta
    84061 acaaattcag aattggcccg tcgtctcaat gtcagtcagg cggcagttac caaggccatt
    84121 aagtctttgg tcaaggaagg gatgttggaa acatctaaag attctaaaga tgcgcgtgtg
    84181 attttttatc agttgactga cttggctcgt ccaatcgctg aggagcacca ccatcaccat
    84241 gagcatacac ttttaaccta tgaacaagtg gcgactcagt ttactccaaa tgaacaaaaa
    84301 gtgattcagc ggtttttgac tgctttagta ggagaaatca aataatgaga tatattacgg
    84361 tagaggattt gtccttctat tatgataagg agcctgttct tgaacatatc aattattgtg
    84421 ttgatagtgg ggaatttgtt accttgactg gggaaaatgg agcggctaag acgacgctca
    84481 tcaaggctag tcttggaatt ctgcaaccac gcattggaaa ggtggctatt tcaaagacaa
    84541 atacgcaagg taagaaattg agaatagcct atcttcctca acaaattgcc agttttaatg
    84601 ctggttttcc aagtacggtc tatgaatttg tcaagtcggg tcgctatccg agaaaaggct
    84661 ggttccgtcg tttgaatgct catgatgagg agcatatcaa ggctagtctg gactcagttg
    84721 gcatgtggga acatcgagac aaacgcttgg ggtctctatc tgggggacaa aagcagcgag
    84781 cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt tatcctagac gagccgacaa
    84841 cggggatgga tgcaggaagt aaaaatgaat tttacgaact catgcaccac agcgcccatc
    84901 atcatggtaa ggctgttttg atgattaccc atgaccctga agaagttaag gattatgcgg
    84961 atcgcaatat tcatctagtc cgtaaccaag actcgccatg gcgttgtttc aatgttcatg
    85021 agaatggcca ggaggtgggc catgcttagt ttattatctt acgactttat acagcgcgcc
    85081 tttttggcgg ttattgctat gagtcttttc tcaccggtat tgggaacctt cctcatcttg
    85141 cgtcgtcaga gtttgatgag tgataccctt agccacgtct cactttcagg tgtagccttt
    85201 ggtctggttt tggggatttc tccaactgtt tctactattg ccattgtctt gattgcggcg
    85261 gtctttctgg agtatctccg tacggtttac aagagcttta tggaaatcgg gacagctatc
    85321 ctcatgtcaa caggtctggc tgtttctctg attgtcatga gcaagggtaa aagctcgagt
    85381 tcaatgagtt tggaccaata tctctttggt tcgatcgtga ctatcagtga agaacaggtc
    85441 atttccctct ttgtcattgc ggcggttgtt ttgattttga cctttctctt tcttcgtcct
    85501 atgtatatct taacttttga cgaagatacg gcctttgtgg atggcttgcc agttcgtacc
    85561 atgtccattc tttttaacat ggtgacaggg gtggctattg cccttatgat tcctgcagca
    85621 ggagctcttc tggtatcgac cattatggtc ttgccagcta gtattgccct gcgtctgggg
    85681 aaaaacttta aatcggttat gctgcttgcc agtgcgattg gctttttggg aatggtagca
    85741 ggactttaca tttcctacta tgcagaaaca cctgcaagtg caagtattac tattattttt
    85801 gtaactgtct ttatactaat cagtttagta agacgtttta tcaaatagga gacgaacgtg
    85861 aaaaaaatta gcttattact agccagtcta tgtgccttgt ttttagtggc ttgttccaat
    85921 caaaaacagg cagatggtaa actcaatatc gtgacaacct tttaccctgt ctatgaattt
    85981 accaagcaag tcgcaggaga tacggctaat gtagaactcc taatcggtgc tgggacagaa
    86041 cctcatgaat acgaaccatc tgccaaggca gttgccaaaa tccaagatgc agataccttc
    86101 gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat tactagatac cttggataag
    86161 aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc tcttgccagg tggcgaggaa
    86221 gaagagggag accatgacca tggagaagaa ggtcatcacc atgagtttga cccccatgtt
    86281 tggttatcac cagttcgtgc cattaaacta gtagagcaca tccgcgacag cttgtcagca
    86341 gattatcctg ataaaaaaga gacctttgag aagaatgcag ctgcctatat cgaaaaattg
    86401 caagccttgg ataaggctta cgcagaaggc ttgtctcaag caaaacaaaa gagctttgtg
    86461 actcaacacg cagcctttaa ctatcttgcc ttggactatg gactcaaaca agtcgcaatc
    86521 tcaggacttt ctccagatgc agagccatca gctgctcgct tggcagaatt gacagagtat
    86581 gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa atgcctcaca agcccttgct
    86641 aacacacttt caaaagaagc aggtgtcaaa actgatgtcc tcaatccttt agaaagtctg
    86701 acagaagagg acaccaaggc tggagaaaat tacatctcta tcatggagaa aaacctcaag
    86761 gctttgaaac aaacaacaga ccaagaaggc ccagcgatcg agcctgaaaa ggcagaggat
    86821 accaagacag tccaaaatgg ttacttcgag gatgcagctg tcaaggaccg caccttgagt
    86881 gactatgcag gtaactggca atcagtttat cctttccttg aagacggcac gtttgaccaa
    86941 gtctttgact acaaggctaa gttgactggt aagatgaccc aggctgagta caaggcttac
    87001 tatacaaaag gctatcagac agatgtgact aagattaaca ttactgataa tactatggaa
    87061 tttgttcaag gtggacaaag caagaaatat acttacaagt atgtcggtaa gaaaattttg
    87121 acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg aagccacaga tgctgacgct
    87181 ggacaattca agtatgttca gtttagtgac cacaatatcg ccccagttaa ggcagaacat
    87241 ttccatatct tttttggagg cacaagccaa gaagccctct ttgaagaaat ggacaactgg
    87301 ccaacctact acccagataa cctatctggc caagaaatcg cccaagaaat gttagcgcat
    87361 tgatgagaaa tcgactagtt cataagagct agtcggtttt ttgatactag atggctaact
    87421 tcattataga cctttca
//
LOCUS       DAVLWA010000002        62972 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLWA010000002 DAVLWA010000000
VERSION     DAVLWA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.002"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            404..1267
                     /locus_tag="VYN80_000088"
     CDS             404..1267
                     /locus_tag="VYN80_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1540070.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            1553..1753
                     /locus_tag="VYN80_000089"
     CDS             1553..1753
                     /locus_tag="VYN80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1540071.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            1820..2485
                     /locus_tag="VYN80_000090"
     CDS             1820..2485
                     /locus_tag="VYN80_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1540072.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            2557..3783
                     /locus_tag="VYN80_000091"
     CDS             2557..3783
                     /locus_tag="VYN80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1540073.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTTLYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4136..4210)
                     /locus_tag="VYN80_000092"
                     /pseudo
     CDS             complement(4136..4210)
                     /locus_tag="VYN80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4281..4937
                     /locus_tag="VYN80_000093"
     CDS             4281..4937
                     /locus_tag="VYN80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1540074.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWHNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGDLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRISVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGMVLDGQ
                     "
     gene            4927..5136
                     /locus_tag="VYN80_000094"
     CDS             4927..5136
                     /locus_tag="VYN80_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1540075.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPTIVERFLKFL
                     PVSIIFALILSRVVTGKVGNLPQIK"
     gene            5354..6184
                     /locus_tag="VYN80_000095"
     CDS             5354..6184
                     /locus_tag="VYN80_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540076.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6338..7192
                     /locus_tag="VYN80_000096"
     CDS             6338..7192
                     /locus_tag="VYN80_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1540077.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7292..8665
                     /locus_tag="VYN80_000097"
     CDS             7292..8665
                     /locus_tag="VYN80_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1540078.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYHTHIELVE
                     ELIRSYE"
     gene            8658..9719
                     /locus_tag="VYN80_000098"
     CDS             8658..9719
                     /locus_tag="VYN80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1540079.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            9721..10413
                     /locus_tag="VYN80_000099"
     CDS             9721..10413
                     /locus_tag="VYN80_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540080.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(10442..11011)
                     /locus_tag="VYN80_000100"
     CDS             complement(10442..11011)
                     /locus_tag="VYN80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1540081.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11146..11760
                     /locus_tag="VYN80_000101"
     CDS             11146..11760
                     /locus_tag="VYN80_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1540082.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            11762..13408
                     /locus_tag="VYN80_000102"
     CDS             11762..13408
                     /locus_tag="VYN80_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1540083.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            13420..14706
                     /locus_tag="VYN80_000103"
     CDS             13420..14706
                     /locus_tag="VYN80_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540084.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            14758..15291
                     /locus_tag="VYN80_000104"
     CDS             14758..15291
                     /locus_tag="VYN80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540085.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            15369..15839
                     /gene="nrdI"
                     /locus_tag="VYN80_000105"
     CDS             15369..15839
                     /gene="nrdI"
                     /locus_tag="VYN80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1540086.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16132..17394)
                     /locus_tag="VYN80_000106"
     CDS             complement(16132..17394)
                     /locus_tag="VYN80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1540087.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(17704..18162)
                     /locus_tag="VYN80_000107"
     CDS             complement(17704..18162)
                     /locus_tag="VYN80_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1540088.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18159..18599)
                     /locus_tag="VYN80_000108"
     CDS             complement(18159..18599)
                     /locus_tag="VYN80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540089.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            18955..20904
                     /gene="mutL"
                     /locus_tag="VYN80_000109"
     CDS             18955..20904
                     /gene="mutL"
                     /locus_tag="VYN80_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1540090.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21229..21696)
                     /locus_tag="VYN80_000110"
     CDS             complement(21229..21696)
                     /locus_tag="VYN80_000110"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1540091.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(21697..22902)
                     /locus_tag="VYN80_000111"
     CDS             complement(21697..22902)
                     /locus_tag="VYN80_000111"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1540092.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLIERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(22922..23557)
                     /gene="ribE"
                     /locus_tag="VYN80_000112"
     CDS             complement(22922..23557)
                     /gene="ribE"
                     /locus_tag="VYN80_000112"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1540093.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(23542..24642)
                     /gene="ribD"
                     /locus_tag="VYN80_000113"
     CDS             complement(23542..24642)
                     /gene="ribD"
                     /locus_tag="VYN80_000113"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1540094.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(24777..24891)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25048..25641
                     /gene="ruvA"
                     /locus_tag="VYN80_000114"
     CDS             25048..25641
                     /gene="ruvA"
                     /locus_tag="VYN80_000114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1540095.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            25651..26214
                     /locus_tag="VYN80_000115"
     CDS             25651..26214
                     /locus_tag="VYN80_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1540096.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     VLLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26211..26888
                     /locus_tag="VYN80_000116"
     CDS             26211..26888
                     /locus_tag="VYN80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1540097.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGP
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(27043..28074)
                     /locus_tag="VYN80_000117"
     CDS             complement(27043..28074)
                     /locus_tag="VYN80_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1540098.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(28329..29012)
                     /locus_tag="VYN80_000118"
     CDS             complement(28329..29012)
                     /locus_tag="VYN80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1540099.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(29024..29968)
                     /locus_tag="VYN80_000119"
     CDS             complement(29024..29968)
                     /locus_tag="VYN80_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1540100.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30100..32931
                     /gene="uvrA"
                     /locus_tag="VYN80_000120"
     CDS             30100..32931
                     /gene="uvrA"
                     /locus_tag="VYN80_000120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1540101.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            32924..33985
                     /locus_tag="VYN80_000121"
     CDS             32924..33985
                     /locus_tag="VYN80_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1540102.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            34117..34515
                     /gene="spx"
                     /locus_tag="VYN80_000122"
     CDS             34117..34515
                     /gene="spx"
                     /locus_tag="VYN80_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1540103.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            34581..35150
                     /locus_tag="VYN80_000123"
     CDS             34581..35150
                     /locus_tag="VYN80_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1540104.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            35237..35503
                     /locus_tag="VYN80_000124"
     CDS             35237..35503
                     /locus_tag="VYN80_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1540105.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            35507..35926
                     /gene="ruvX"
                     /locus_tag="VYN80_000125"
     CDS             35507..35926
                     /gene="ruvX"
                     /locus_tag="VYN80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1540106.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            35942..36247
                     /locus_tag="VYN80_000126"
     CDS             35942..36247
                     /locus_tag="VYN80_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1540107.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            36505..37755
                     /locus_tag="VYN80_000127"
     CDS             36505..37755
                     /locus_tag="VYN80_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1540108.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIAEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            37752..38294
                     /locus_tag="VYN80_000128"
     CDS             37752..38294
                     /locus_tag="VYN80_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1540109.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            38440..39972
                     /gene="cls"
                     /locus_tag="VYN80_000129"
     CDS             38440..39972
                     /gene="cls"
                     /locus_tag="VYN80_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1540110.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            40230..41768
                     /locus_tag="VYN80_000130"
     CDS             40230..41768
                     /locus_tag="VYN80_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1540111.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWGLHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            41888..44095
                     /gene="nrdD"
                     /locus_tag="VYN80_000131"
     CDS             41888..44095
                     /gene="nrdD"
                     /locus_tag="VYN80_000131"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1540112.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMN
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            44107..44247
                     /locus_tag="VYN80_000132"
     CDS             44107..44247
                     /locus_tag="VYN80_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540113.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            44291..44797
                     /locus_tag="VYN80_000133"
     CDS             44291..44797
                     /locus_tag="VYN80_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540114.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            44790..45380
                     /gene="nrdG"
                     /locus_tag="VYN80_000134"
     CDS             44790..45380
                     /gene="nrdG"
                     /locus_tag="VYN80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1540115.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            45377..45994
                     /locus_tag="VYN80_000135"
     CDS             45377..45994
                     /locus_tag="VYN80_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1540116.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            46260..46568
                     /gene="rpsJ"
                     /locus_tag="VYN80_000136"
     CDS             46260..46568
                     /gene="rpsJ"
                     /locus_tag="VYN80_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1540117.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            46785..47411
                     /gene="rplC"
                     /locus_tag="VYN80_000137"
     CDS             46785..47411
                     /gene="rplC"
                     /locus_tag="VYN80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1540118.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            47436..48059
                     /gene="rplD"
                     /locus_tag="VYN80_000138"
     CDS             47436..48059
                     /gene="rplD"
                     /locus_tag="VYN80_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1540119.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            48059..48355
                     /locus_tag="VYN80_000139"
     CDS             48059..48355
                     /locus_tag="VYN80_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1540120.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            48373..49206
                     /gene="rplB"
                     /locus_tag="VYN80_000140"
     CDS             48373..49206
                     /gene="rplB"
                     /locus_tag="VYN80_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1540121.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            49310..49591
                     /gene="rpsS"
                     /locus_tag="VYN80_000141"
     CDS             49310..49591
                     /gene="rpsS"
                     /locus_tag="VYN80_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1540122.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            49603..49947
                     /gene="rplV"
                     /locus_tag="VYN80_000142"
     CDS             49603..49947
                     /gene="rplV"
                     /locus_tag="VYN80_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1540123.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            49960..50613
                     /gene="rpsC"
                     /locus_tag="VYN80_000143"
     CDS             49960..50613
                     /gene="rpsC"
                     /locus_tag="VYN80_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1540124.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            50617..51030
                     /gene="rplP"
                     /locus_tag="VYN80_000144"
     CDS             50617..51030
                     /gene="rplP"
                     /locus_tag="VYN80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1540125.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            51040..51246
                     /locus_tag="VYN80_000145"
     CDS             51040..51246
                     /locus_tag="VYN80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1540126.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            51271..51531
                     /gene="rpsQ"
                     /locus_tag="VYN80_000146"
     CDS             51271..51531
                     /gene="rpsQ"
                     /locus_tag="VYN80_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1540127.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            51557..51925
                     /gene="rplN"
                     /locus_tag="VYN80_000147"
     CDS             51557..51925
                     /gene="rplN"
                     /locus_tag="VYN80_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1540128.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            52003..52308
                     /gene="rplX"
                     /locus_tag="VYN80_000148"
     CDS             52003..52308
                     /gene="rplX"
                     /locus_tag="VYN80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1540129.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            52332..52874
                     /gene="rplE"
                     /locus_tag="VYN80_000149"
     CDS             52332..52874
                     /gene="rplE"
                     /locus_tag="VYN80_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1540130.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            52892..53161
                     /gene="rpsN"
                     /locus_tag="VYN80_000150"
     CDS             52892..53161
                     /gene="rpsN"
                     /locus_tag="VYN80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1540131.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            53446..53844
                     /gene="rpsH"
                     /locus_tag="VYN80_000151"
     CDS             53446..53844
                     /gene="rpsH"
                     /locus_tag="VYN80_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1540132.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            54036..54572
                     /gene="rplF"
                     /locus_tag="VYN80_000152"
     CDS             54036..54572
                     /gene="rplF"
                     /locus_tag="VYN80_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1540133.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            54656..55012
                     /gene="rplR"
                     /locus_tag="VYN80_000153"
     CDS             54656..55012
                     /gene="rplR"
                     /locus_tag="VYN80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1540134.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            55030..55524
                     /gene="rpsE"
                     /locus_tag="VYN80_000154"
     CDS             55030..55524
                     /gene="rpsE"
                     /locus_tag="VYN80_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1540135.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            55496..55720
                     /gene="rpmD"
                     /locus_tag="VYN80_000155"
     CDS             55496..55720
                     /gene="rpmD"
                     /locus_tag="VYN80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1540136.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            55865..56305
                     /gene="rplO"
                     /locus_tag="VYN80_000156"
     CDS             55865..56305
                     /gene="rplO"
                     /locus_tag="VYN80_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1540137.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            56318..57628
                     /gene="secY"
                     /locus_tag="VYN80_000157"
     CDS             56318..57628
                     /gene="secY"
                     /locus_tag="VYN80_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1540138.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            57779..58417
                     /locus_tag="VYN80_000158"
     CDS             57779..58417
                     /locus_tag="VYN80_000158"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1540139.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            58534..58752
                     /gene="infA"
                     /locus_tag="VYN80_000159"
     CDS             58534..58752
                     /gene="infA"
                     /locus_tag="VYN80_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1540140.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            58777..58893
                     /gene="rpmJ"
                     /locus_tag="VYN80_000160"
     CDS             58777..58893
                     /gene="rpmJ"
                     /locus_tag="VYN80_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1540141.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            58911..59276
                     /gene="rpsM"
                     /locus_tag="VYN80_000161"
     CDS             58911..59276
                     /gene="rpsM"
                     /locus_tag="VYN80_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1540142.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            59294..59677
                     /gene="rpsK"
                     /locus_tag="VYN80_000162"
     CDS             59294..59677
                     /gene="rpsK"
                     /locus_tag="VYN80_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1540143.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59720..60655
                     /locus_tag="VYN80_000163"
     CDS             59720..60655
                     /locus_tag="VYN80_000163"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1540144.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            60667..61053
                     /gene="rplQ"
                     /locus_tag="VYN80_000164"
     CDS             60667..61053
                     /gene="rplQ"
                     /locus_tag="VYN80_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1540145.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            61318..61584
                     /locus_tag="VYN80_000165"
     CDS             61318..61584
                     /locus_tag="VYN80_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1540146.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            61594..62931
                     /locus_tag="VYN80_000166"
     CDS             61594..62931
                     /locus_tag="VYN80_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1540147.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        18771 a        11880 c        13832 g        18489 t
ORIGIN      
        1 gagattaact attgatatta aaaaggcaat aatttataaa atttgtacaa aaaaatgtta
       61 aattttcaca ttttcatagt atactgttta atagaaagta ggttctttgt aaccgctcca
      121 ttataaaatc ctttcactct tccggtatac tattaggaaa aacataactg gaggatattt
      181 tgagctcaca cctcatcgtt ccagtggctg ttccccaagc tcgtgtactg cccaaaaaag
      241 ttagacattc tatttagaac gaggattgag ttctgtattg tacaaggctc ggtcctttta
      301 gagtcagctt aaggctggct tttttatttt ttctaactat cagaatattt tgacaaatga
      361 acacaaataa tgatataata tgcaaaagct aggaggtggt aggatgattg aaaaaatgga
      421 attgggggaa ttttacaagg aattacgttt ggcgagaaag ctcaagcagt cagatgtggc
      481 ttgtgacggg ctaacagctt ctcagttgtc caagtttgag ctagggcagt ccatgttgtc
      541 tgctgataag ctcatattag ccatccaagg gatcaatgtg acctttgatg agtttgggca
      601 caagctcaac aactatcaag aatctccaca tatgcagatt ggtagaaagg ttgtggatcg
      661 ctttgctcat caagatatag ctggcttaga gcaactgttg gaggaagtcg agcaagaaca
      721 gatggcagag acttatcgtc gtttgaatgc tattgtaatc aaagatgcca tccactcact
      781 agataaaagc tacccactag cagaggagga tagcgagttt ttgaccacct atctctatgc
      841 tatcgagtct tggacctggt ttgaactcta tctcttttgc aatactctgc ccttcttgag
      901 caatcaagat ctgatctttt tatcaacctc cttactcgaa aaatccaaag aatttaaaga
      961 gttagtacac aatcgattgt atatgaagca aggactctta aatatcatct cagagctcat
     1021 ggagcgcaaa cttttttctt atatcccaat ctttgaagcc gagctggaga gtatgctccg
     1081 tccatacgat gtttttgaga aagtgttgtg gcagttttta aagaagatga gcattttcct
     1141 tcaaactaag ggaagcaatc aaaaagagat tgaacacttt atccaatctc tgcaagtatt
     1201 agaaaatcca caattaatag ccctttttga attgcgtttg cagcaataca aaaagcttat
     1261 cgattagtta gaaatggcaa aataataata atctcagcaa cgattttcaa taatgttaaa
     1321 atttgtttct tcatatttct ccactcctca ttagtagtaa ctttattata ccagagaagt
     1381 aatcaaattc accaaaatcg caaatctgaa atttctataa gaaaaaatat caaatatgcg
     1441 attttctaaa ataagccaat tttcgtgtta tactgtactt gtaaagcact tgaagcaaat
     1501 cctaggtcgc agaagtggtt tacaaatgaa gataattgaa ggagtgtaaa agatgttaaa
     1561 tttacaattt gcagaaacaa tggaattgac agaagctgag ttggagatag tttatggagg
     1621 ggaatttggg aataatgctg ttatcccagc cggtgcttgg ggagggttcg ggacaccttg
     1681 gtcaatcact aatttctgga agaaaaattt taacgataga cctgactttg attctgaccg
     1741 ccgtcgttat taatatccaa agcccttgcc ttttcttggg taggggctgt ctttatttca
     1801 tagtatagga ggaaatagaa tgaaaaagat aatctcacac cgctacttta ttatagtttt
     1861 tctactagtt attgctgacc agaagttcag tgttctagtt ttacgcagcg accttgttac
     1921 tgggctatct gactttgcct attatctgtc ggatatgatg ttgaattttc ttgtggtttt
     1981 atttgctctt attgctatga tttggtcggg gaaatggcag aaaatcaata gtagaaagtt
     2041 taaaggatcc tatcttttct attccttcct agctctgctt gcttttgttg tttggaattt
     2101 cgttaccttt tttcttttcc cacctactcg aaatgaaatt tcttatcaac atgctgctcc
     2161 gacttttaca ggggctacag catttttgat gtattttttc tatcctgtga ttgcaggtcc
     2221 catttttgaa gagataatcc atcggggatt ggtgatgaca gctctggaaa aaggaaagaa
     2281 atgggggcta gatgtacttg gctctgctat tttatttggg gtctcgcaca ttagtaatca
     2341 cggttgggtc ttgacagact ttgtcttcta tatgggtgga ggtcttatat ttgcagtctt
     2401 atttagaatg acaaagtcaa tttattggcc tattggactg catatagtct acaatggtat
     2461 tggtcagctt ttgatgttac tgtaattttg gttgttagta gtatcgcggt ctattcttgg
     2521 ggaaagattc ccatttgaac ttaagaagga gttgtaatga aagtatttct tcaaaataga
     2581 gattttaggc aattaactat caaccagtgg atttcaacgc ttggggatac gattttttat
     2641 ctggccttcc tgaattatgt ggcagatgcg tcttttgccc ctttggcgat tttacttatc
     2701 acgatttcag aaacccttcc ccaagttcta caaatctttc tgggagtttt ggcggatttt
     2761 caacatcatc gtgtcttgaa atacacagtc attagtttta caaaattttt gctttactct
     2821 atagtgtccc tatcgctttc agggcagtct ttttccttgt tgctagtaac ctttatttgt
     2881 atgattaacc tcttgtctga cacattgagt tatttttcag gcgccatgct cactccgatt
     2941 ttcattagaa ttattgggca agatcatctg gcagaagcta ttggatttaa acagtcaact
     3001 gttagtttag tgaaaacaat cagtaatatc ctaggaggag tcttactagg tattctatcc
     3061 atccagttta tttccttact gaacgctctt acctttttaa tcgcattttt aggtatcctc
     3121 ttcataaaaa ctgacctctt gaaagtagaa aaaacgatta gctatcaaga aggactctct
     3181 gtaaaatcct tttgccagca tttgctccaa tcatcgaaat tgatatggaa tatgaataag
     3241 gtgctcttgg ttttgtttat tatctctact agtcaagcag tgataaatgt tacagttcct
     3301 gtctctactc tatttttgag gaatcaaccc tttttgaatt tacaaacagg tcaatctctt
     3361 gccttgctat ccacttttga actgtcagcc ctgattgtcg gaagccttgt aagtggctat
     3421 ctgcaacata ccatttccat aaaaacaact ctctatgcct cacttgtcat ccagttgctt
     3481 cttctagtag gatttgccac agttcgtttt gactggattc tcatctttag taccttggat
     3541 gcctttttcg caggtgttct ctctcctaga ttgcaagaac tcgtctttaa acaaatacct
     3601 gaggagtcaa tgggagcagt tcaatcttct atcagtgcca ttacggttgt tttacctagc
     3661 ttatttacaa tatctttggt aaccattgct actagctttg gaactctggc agttagcttt
     3721 gttttattgc tatttcttct agttgccttt gttatgcttc tgaatattcg tgaaagtatt
     3781 tagtggagaa tttctataat atatgtttga gactggattg ccagtctttt tatgatggtc
     3841 aaaatcaaca gactttttct cttttctgaa tgtgttatag tagactgaat ctaaaatagt
     3901 acgaaacaat tgctaaaaca tttatagaaa ttaattttac tttcccaatc gatttgttct
     3961 catcttattt caatctgcta tataatagtc catgctgtgg ctggattctt ttcagcctac
     4021 ttatactctt cgaaactctc ttcaaaccac gtcagcttca ccttgccgta tatgtgttac
     4081 tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact tcatcagttc
     4141 tatctgcaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctctt
     4201 tgattttcat tgagtattat taagacaata tgaggagaaa gatgaaagaa aaaggatttt
     4261 aggagggggc gcaggcagcc atgccaacgg ctcttggcta tgtcagtatc ggcctggcct
     4321 gtggaattat cggtgcgccc tatgtgacac ctgttgagat gggcttgatg agtctctttg
     4381 tttatgctgg gagtgcccag tttgccatgt tggcactgat tgtggttcaa gctcctgtgg
     4441 cagctattgc tatgacggtt tttctaatca atttgcgtct ctttttgttg agcttacacg
     4501 catcgactta tttccgtcat accagtcttt ggcacaatat cggtatgtct agtatcttga
     4561 cagatgagac ctatggcgtt ttgatgggcg aattagccca tacagataag gtcaatccga
     4621 tgtggatgca cgggaacaat ctcaacagct atgtggcttg gtttgtggga acagtggtcg
     4681 gaacggctct aggtgacctg ttgccaaatc cagaaatctt tggtttagat tttgccctgg
     4741 ttgggatgtt tatcgggatt tttgcttcgc aattccagat gatgcaaaga cggatttctg
     4801 tccgcaatct gctcattatc ttagcagttg ttgcagtgtc cttcttttta cttttgacag
     4861 tggtgtctca gtcactagct gttctgtttg cgacgctact tggttgtagc atggggatgg
     4921 ttttagatgg tcagtaagta tcttttatta gcagttattt tctctggtct tgtgacctgg
     4981 attccccgta tgattccctt catcttggtc aagtataagg gcctgcctac gattgttgag
     5041 cgttttttga agtttttgcc tgtttccatt atctttgcct tgattctttc aagagtagtg
     5101 acaggtaagg ttgggaacct tcctcaaatc aaatgactgg acttcttagc agtctttcca
     5161 acagcttggg tagcttttcg ctaccgcaat ctagtcggta cggttctctt tggagtagtc
     5221 ttgattgccg tcttgcgttt ggtcttttaa ataagtcata aagaaaactt atcacagaga
     5281 tagatatcat ataatggcgt aattactttt tttctgataa gattataagg tattctattt
     5341 tggaggaaat gacatgaaaa aaatcgttaa atactcatct cttgcagccc ttgctcttgt
     5401 tgctgcaggt gtgcttgcgg cttgctcagg tggtgctaag aaagaaggac aagcagctag
     5461 caagaaagaa atcatcgttg caaccaatgg atcaccaagg ccatttatct atgaagaaaa
     5521 tggcgaattg actggttacg agattgaagt cgttcgcgct atctttaaag attctgacaa
     5581 atatgatgtc aagtttgaaa agacagaatg gtcaggtgtc tttgctggtc ttgacgctga
     5641 ccgttacaat atggctgtca acaatcttag ctacactaaa gaacgtgcgg agaaatacct
     5701 ctatgccgca ccaattgccc aaaatcctaa tgtccttgtc gtgaagaaag atgactctag
     5761 tatcaagtct ctcgatgata tcggtggaaa atcgacggaa gtcgttcaag ccactacatc
     5821 agctaagcag ttagaagcat acaacgctga acacacggac aacccaacta tccttaacta
     5881 tactaaggca gatttccaac aaatcatggt acgtttgagc gatggacaat ttgactataa
     5941 gatttttgat aaaatcggtg ttgaaacagt gatcaagaac caaggtttgg acaacttgaa
     6001 agttatcgaa cttccaagcg accaacaacc gtacgtttac ccacttcttg ctcagggtca
     6061 agatgagttg aaatcgtttg tagacaaacg catcaaagaa ctttataaag atggaactct
     6121 tgaaaaattg tctaaacaat tcttcggaga cacttatcta ccggcagaag ctgatattaa
     6181 ataatttctt gaaatcatcc attctgagta agatgggtgg tttctcagtt tttagggata
     6241 tagttttaaa ctatatatct gactatctaa taacaatttg tgaaatcaaa caaattgtga
     6301 gatactagta cggtattatt tttaaggaga aagaatcatg aaaatcaaaa aatggcttgg
     6361 tctagcagcc cttgctacag tcgcaggttt ggctcttgca gcttgcggaa actcagaaaa
     6421 gaaagcagac aatgcaacaa ctatcaaaat cgcaactgtt aaccgtagcg gttctgaaga
     6481 aaaacgttgg gacaaaatcc aagaattggt taaaaaagac ggaattacct tggaatttac
     6541 agagttcaca gactactcac aaccaaacaa agcaactgct gatggcgaag tagatttgaa
     6601 cgctttccaa cactataact tcttgaataa ctggaacaaa gaaaacggaa aagatcttgt
     6661 agcgattgca gatacttaca tctctccaat ccgcctttac tcaggtttga atggaagtgc
     6721 caacaagtac actaaagtag aagacatccc agcaaacgga gaaatcgctg taccaaatga
     6781 cgctacaaac gaaagccgtg cgctttactt gcttcaatca gctggcttga ttaaattgga
     6841 tgtttctgga actgctcttg caacagttgc caacatcaaa gaaaatccaa agaacttgaa
     6901 aatcactgaa ttggacgcta gccaaacagc tcgttcattg tcatcagttg acgctgccgt
     6961 tgtaaacaat accttcgtta cagaagcaaa attggactac aagaaagcac ttttcaaaga
     7021 acaagctgat gaaaactcaa aacaatggta caacatcatt gttgcgaaaa aagattggga
     7081 aacatcacct aaggctgatg ctatcaagaa agtaatcgca gcttaccaca cagatgacgt
     7141 gaaaaaagtt atcgaagaat catcagatgg tttggatcaa ccagtttggt aataagaaac
     7201 agggaggtgg gagagaaaat tccacctctt gcttttgtat agagtataga ttgtaaagaa
     7261 gactattcgt tcatagaaag gtagagagaa tatggttttt cctagcgaac aagaacagat
     7321 tgaaaaattt gaaaaggatc atgtagtcca acattatttt gaggttttgc gtaccttgat
     7381 ttctaagaaa tcagtctttg cccagcaggt tggactcaag gaagtcgcaa attatctggg
     7441 tgagattttc aagcgtgttg gagctgaagt ggagattgat gagagctata cagcgccctt
     7501 tgtcatggca catttcaaga gttcgcgtcc agatgccaag accttgattt tctataacca
     7561 ctatgacact gtgccagcgg atggggatca ggtctggaca gaggatcctt ttacgctttc
     7621 ggtccgcaat ggcttcatgt atgggcgtgg ggttgatgac gacaagggtc atatcacagc
     7681 tcgcttgagt gctttgagaa aatatatgca gcaccatgat gatttacctg tcaatatcag
     7741 ctttatcatg gagggagcgg aggaatcggc ttcaacagac ctagataagt atttggaaaa
     7801 gcatgcagac aaactccgtg gggcggattt gttggtctgg gaacaaggga ccaaaaatgc
     7861 cttggagcag ctggaaattt ctggtggcaa taaggggatt gtgacctttg atgccaaggt
     7921 aaaaagcgct gatgtggata tccactcgag ttatggtggt gttgtggaat cagctccttg
     7981 gtatctcctc caagccttac agtctcttcg tgctgcggat ggccgtatct tggttgaagg
     8041 cttgtacgaa gaagtacaag agcccaatga acgagaaatg gccttgctag aaacttatgg
     8101 tcaacgaaac ccagaggaag ttagtcggat ttatggattg gagttgcctc tcttacagga
     8161 ggagcggatg gcctttctaa aacgtttctt tttcgagcca gcgcttaata tcgaaggaat
     8221 ccagtctggt tatcaaggtc agggtgttaa gactatttta cctgcagaag ccagtgccaa
     8281 gctagaggtt cgtctggttc cgggcctaga accgcatgat gttctggaaa aaattcggaa
     8341 acagctagac aaaaatggct ttgataaggt agaattatac tataccttgg gagagatgag
     8401 ctatcgaagc gatatgagcg caccagccat tctcaatgtg atcgagttgg ccaagaaatt
     8461 ctatccacag ggcgtttcag tcttgccgac gacagcgggg acaggaccta tgcatacggt
     8521 ctttgatgcc ctagaggtac caatggttgc attcggtcta ggaaatgcca atagccgaga
     8581 ccacggtgga gatgaaaatg tgcgaatcgc tgattatcac acccatatcg aattagtaga
     8641 ggagctgatt agaagctatg agtagagata ttatcaagtt agatcagatc gatgtgactt
     8701 ttcaccaaaa gaagagaacc attacagcgg ttaaggatgt gaccattcac atccaagaag
     8761 gggatatcta cggaatcgtt ggatattctg gagcaggaaa atcaaccctt gtacgggtga
     8821 ttaatctctt gcaaaaacca tctgcaggga aaattaccat tgacgacgat gtgatttttg
     8881 acggcaaggt gaccttgacg gcagagcagt tgcgtcgtaa acgtcaagat atcggaatga
     8941 ttttccagca ttttaacctg atgagccaaa agacagcaga ggagaatgta gcctttgccc
     9001 ttaaacactc tgaactcagc aaggaagaaa agaaggctaa agtagctaag ttgttggact
     9061 tggttggttt ggcagatcgt gctgaaaact acccttcaca actatctgga gggcaaaaac
     9121 agcgtgtggc aattgcgcgt gccttggcca atgatccaaa aatcttgatt tcagacgagt
     9181 caacttctgc ccttgatccg aagacaacca agcagatttt ggccttgttg caagatttga
     9241 accaaaaatt aggcttgact gttgtcttga ttacgcatga aatgcagatt gtcaaagaca
     9301 ttgccaaccg tgtggcggtt atgcaggatg ggcatttgat tgaagagggt agtgtgcttg
     9361 aaatcttctc agaccctaaa caacctttga ctcaagactt tatctcaaca gctacaggta
     9421 ttgacgaagc catggtcaaa atcgagaagc aagaaatcgt ggaacacttg tctgaaaaca
     9481 gtctcttggt gcaactcaag tacgctggat cttcaacaga cgagccactt ttgaatgaat
     9541 tgtacaagca ttatcaagta atggctaata ttctctatgg gaatatcgaa atcctcgatg
     9601 gtactcctgt tggagaattg gtggtggtct tgtcaggtga aaaagcagcg ttggcaggtg
     9661 cccaagaagc cattcgtcaa gcaggcgtac agttaaaagt attgaaggga ggacagtaag
     9721 atggaatcat tgattcaaac ctatttgcca aatgtctata agatgggttg gtctggtcag
     9781 gcaggctggg gaacagctat ctacctaacc ctctatatga cagttctttc cttcattatc
     9841 ggaggcttct tggggctagt ggcaggtctc tttctcgtct tgacagcgcc aggtggtgtc
     9901 ttggagaata aagtcgtatt ctggatttta gacaaaatta cctcaatttt tcgtgcgatt
     9961 ccctttatca tcctcttggc aatcttgtca ccactttctc acttgattgt taaaacaagt
    10021 atcgggccaa atgcagccct tgtcccactt tcttttgcag tctttgcctt ctttgcccgt
    10081 caggtgcagg ttgtcttggc tgaattggat ggcggtgtca ttgaggcggc tcaagcgagc
    10141 ggagcgactt tctgggacat cgtgggtgtt tacctatcag aaggtcttcc agatttgatc
    10201 cgtgtgacga ctgtgacctt gatttccctt gttggggaaa cagctatggc cggtgcggtt
    10261 ggagctggtg gtatcggtaa cgtagccatc gcttatggat ttaaccgcta caatcacgat
    10321 gtgaccatct tggcaaccat tattatcatt ttgattatct ttgcaatcca attcttggga
    10381 gatttcttga ctaagaaatt gagtcataaa taaaaagagc cgcgtggctc tttttaactg
    10441 atcagatttt ctgtgcaaat tttttactca aggcttgtcc aatcaaggca cccactaggg
    10501 ctccgatgac aatacttgcg ataaatagaa ggacagttcc agggtttgga gcgaccatga
    10561 tacggtcgat atattcttgg gattttcctc ttgccagaag agtagccata taggctttgg
    10621 gcgcaatcca cataagcaag attggtcctg ttgtactaaa ggcgaaaata atgaaagaaa
    10681 ggaagttctt tgttttgtcc ttgtattttc ctaaatgagc tacttcatct gctaggaggc
    10741 cacagataat tccaggaagg aaggaaccag caccgtgttt agttcccaag aaaaagaggg
    10801 caatgacaag gccgatagtg gtaatggctc caaagcgcgg aacttttgcg actaggatca
    10861 tatagacgct accgccgaca agggcagtaa aggcaggcgc ataaaacatg tttccagttt
    10921 ggtcaaagag attgcccaaa aggacaccaa tccccatgca gaggaagtaa agaactgcaa
    10981 aaagcagtgt agttaagata gatttcttca tgaattgtct cctgataatt ttttcacaat
    11041 tctcattgta ccacggtttg gtgggattgt aaatggatac tagaagtttt tgaaaacgct
    11101 tgaaatatga tataatggtt tcatagttat ttttagggga atatcatggg tagattttta
    11161 gactttgtct ttaatcgttt ctttttaggg atgattgcga cagccttctt ttggctatta
    11221 actttagcag gagggattat ccttggtcta gcgccggcta gtgccacctt gatgagctta
    11281 tatgcagaac atggttatag ctttcgggaa tacagtttga aggaggcttg gtctctttac
    11341 aagcaaaatt ttgtctcaag caacctgatt ttctatagct ttttaggtgt gggtctagtt
    11401 ttgacctatg gtttgtatct cttggtgcaa ttgcctcatc agaccattgt tcatttgatt
    11461 gcgacccttt tgaatgtcct agtagttgcc ctgatctttt tggcttatac agtatcttta
    11521 aaattacaag tttattttgc cttgtcctat cgaaatagtc tcaaattatc cttgattggc
    11581 atctttatga gtctagcagc tgtggccaag attctccttg ggactgtgct acttgtagca
    11641 attggttact atatgcctgc cctgctattt tttgtaggaa ttgggatgtg gcatttcttt
    11701 atcagtgata tgttggaacc tgtctatgaa atcatccatg aaaaattggc gacaaaatag
    11761 aatgaagcag ttttggctac atacgcttct aagaacctat agttcagtga tgatcattat
    11821 cattgcgagt tttgcaatct tactctctta cgctgactgg gattcacgtg aaaaggaagc
    11881 ccagagagta gcccagcgtg taactgctag aacagtgagt gaaattgaat attaccatag
    11941 agagtcaacc cagatagctc aggctttagt tgaaaaccaa gctcgtattg agggaatcta
    12001 taaatacttt agtcttagca tgccagacta tttttactgg caattagagc ggaaagcttc
    12061 gccttatata tcagtctctc tgtatgaaaa tgttgatgac ctctatgttc gaaatgattt
    12121 tgtaactgga gtggccattg cttttcaaga ttacaaggaa gtctatgttt ctactaaaga
    12181 caaacgtagt ggagaaaaaa tcagggctga ggatttcaaa ccagcaggaa atagttttgc
    12241 cattccggtg tcagatccag tgtcagatca agatttagga gtgatttaca tctccttgga
    12301 tcctgctgtt ttataccatg ccattgataa tactagaggt catactccga tggcagtaac
    12361 agtgacctca ccttttgata cggagatttt tcatattggt gagacagttg ataaggagag
    12421 tgaaaattgg ctagttggct taacttctca tggatatcag gttcaggtgg cagttcctaa
    12481 aaactttgtt ttacaaggaa cagtgactag ctctgctttg attgtgggtt tgagccttct
    12541 ctttattgtc attctttatc tgactttgag gcagactttt gctaattacc aaaagcaggt
    12601 agtggattta gtagaatcca ttcaagtcat tgctcaaggc gaagaggggc gtcggattga
    12661 catttccgag aaagatcagg aattactcct aatcgcggag acgaccaatg atatgttgga
    12721 tcgattggaa aagaatatcc atgatattta ccagttagag cttagtcaaa aagatgccaa
    12781 tatgcgagcc ttgcaggcgc aaatcaatcc tcattttatg tataatacgc tggagttctt
    12841 gcgcatgtat gcagttatgc agagtcaaga tgagttggca gatatcattt atgaattcag
    12901 tagtctcttg cgtaacaata tttccgacga aagagagacc ctcctcaaac aggaattaga
    12961 attttgccgt aaatacagct atctctgcat ggttcgctat cccaagtcca ttgcctatgg
    13021 tttcaagata gatccagagt tagagaatat gaagattccc aagtttacct tgcaaccgct
    13081 ggtagaaaac tatttcgcgc atggtgttga ccacaggcgg acagataatg tgattagcat
    13141 caaggctctt aaacaggatg gttttgtgga aattttggtg gtcgataatg gtagaggaat
    13201 gtcggctgaa aagttggcaa atatccgaga aaaattaagt cagagatatt ttgaacacca
    13261 agccagctac agtgatcaaa ggcagtctat cgggattgtc aatgtacacg agcgttttgt
    13321 gctctatttt ggagaccgct atgccattac gatagagtct gcagagcaag ccggtgttca
    13381 gtatcgtatt acaattcaag atgagtagaa agggagaaaa tgtataaagt attattagta
    13441 gatgatgagt acatggtgac agaaggtctg aagcgtttga ttccctttga taagtgggat
    13501 atggaggtcg tcgcaacagc cagtcatgcc gatgaagctc tagaatatgt tcaggaaaat
    13561 cctgtcgatg tcatcatttc cgatgtcaat atgccagaca aaacagggct tgatatgatt
    13621 cgggagatga aagagatctt accagatgct gcctatatcc tgctctcagg ttatcaggag
    13681 tttgattatg taaaaagagc aatgaacctt agtgtggtgg actatttggt caagcctgtt
    13741 gataaggtag agctgggaaa tctgctggag aagattgcag gtcagctcgg cgagagaggg
    13801 aagaaaagtc agactcttag tcaagaatta gacgaggctg gatttgttag ttatttaggg
    13861 gataaggaga attggtggat aggtctatcc aaggaaaaac aaggttcctt cgccattccc
    13921 tactatgtct tgggccaaga ctggcagatt ttcatttctg accaacccct agatggttta
    13981 gtcgttacac cttttgaagc tccttatcaa gaacactttg aacgctggaa gctgaatgct
    14041 gagaaaaccc tcttttacgg ttctgtaaat ctgcagcagt ctgagagtct ctttgcctat
    14101 tacgaaccga tttatagggt tatcattcag ggaaatctca atcaaatcgt agaagagtta
    14161 aatctcttgg agaaggtagt tcttgaaaat acgccgcgtg ttccgattac taaacagctt
    14221 tttattcagt ttgtcatgga tgttttccat ttatttgaac atctcaaagc tgatgacctg
    14281 acagacatcg tcaaaaccat tcatgctatt caatccttcg atgaattggt tccttatatc
    14341 aaggaaactc tgaccagctt tttcggtcaa taccgtatga atgaaaatgt ggtcagtgtg
    14401 ctggaagtca ttggtcgtga ttaccaaaaa gagctttccc tcaaggatat cagtaaggct
    14461 ctctttatca atcctgtcta tctagggcag ttgattaagc gtgaaaccga ttcgactttt
    14521 gcagagttac taaacaaaca acgtattaag gctgcccaac aacttttgct ttcaactagt
    14581 gacagcatcg aagatatttg ttatgctgtt ggttacagta accttggata tttctataaa
    14641 gtttttcgaa aattgtgcgg aaaatcgcca aaagcctacc gaaaacaggt agaaactata
    14701 ctataagatt tgtattcctt tacaaaaggt gctataatat tatcaggagg ttctatcatg
    14761 aaaaagaaac caatttatct atgggtcttg ctaatcttgt ctgcccttat ttcagttccg
    14821 tctctgtttg gaattgtgag tcccttgcct agtaaagaag cccttcgtgc tgctcagaaa
    14881 caagttgcag gggtcaatgc tcagcaatta gaagaccagc ttaattatac ctatagagta
    14941 gcagaagcat ctcattctat ttttaatgtt gctttgattg tgctatctac tattttagtg
    15001 gtcgtagcga ttgtcttcct tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt
    15061 ggttatgttt tgttagctat tattggttcg atttatggct atgttggttt acaagacgct
    15121 gtgcagttgg ttcaagatga gagcatgcgt ttgacagtga gtattggttc aaaagctgtt
    15181 agcattttct atatcgtcat caatgttctc ttcctagccc ttgtcttcta taagatgtgg
    15241 cgacaacaga aagccttggc agaagaagag gaaacagaag agcttaccta agtattgaca
    15301 aaaaagaact atcaagatac aatcttggta gttgtttttt gattatagat aaaatcatgg
    15361 aggtacatat gaagacaatt tctttagttt atattagttt gagtggcaac actgaaagtt
    15421 ttgtgacgcg cttgaaagac tatctcttgt cccagtacaa agggattgag gttcaaaaga
    15481 ttcatatcaa ggatttagtc aaggaaggca aaaatttcta tgaaatggac catccctatg
    15541 tcgctttttt gccgacctat ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga
    15601 ttttgacgac accggtggga gattttattg cctatggtaa caatgctagt aagtgttttg
    15661 gtgtggttgg gtcaggaaat cgtaacttta ataaccaata ctgcctaaca gccaagcaat
    15721 acagtcaacg ctttggcttc cctgtattgg ctgactttga aatgcgaggc atgctggaag
    15781 atatcaaaca tgtcgcagct attatcgcag atttgtatga gttggaaaag gagaattaat
    15841 actcaatgaa aatcaaagag caaactagga agctagtcgc aggttactca aagcacagct
    15901 ttgaggttgc agatagagct gacgtggttt gaagagattc tcgaatagta taaagttctt
    15961 ataagaggtg gttgaagttg ttcaactgtt ttttgagtat aaacagtctt taagaacgta
    16021 aaaaagcata agttcaggtc ttcaaaacct tgttcttatg ctttttatca tagaaatatt
    16081 tgctgaattt tctcttgaaa tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc
    16141 ttttccgatt gcgagtgcgt agataaagaa gatggcgaag ccaaagagga aaatcaatcc
    16201 cgcaatggca aggtgtttga gccaagaagg aagatttttg ttttcacgcg ttaccaaggc
    16261 gtaattcaaa agagcaaaga aaggtgttgt caggaaagag ccaatcatgg caaagcggag
    16321 catggttgaa acctgaccag cgaagaactt gataatgacg ataccgatga tagcagtgat
    16381 ggtcatccag atgttcaaag atttacgatt gtcctctttt tgactgatta gcagtcggag
    16441 agattcctga ttaacgcgag aatagccatc gataacagtt ataactgttc caaagataca
    16501 gaggaaggca ataaaggtaa tcaagtaacg ggaccattcg ccaagaacag aggcatacat
    16561 acccacgaat tgagagatgt atttggctga agcagcttca accgcctgcc ctgtaggata
    16621 ctgaatcagt gctcccagtg ccacaaagaa gacggctagg atagctgttc caatataacc
    16681 agtgttaaag tcaaacagag cgtcttctgt gttaaagttg acggtctttc tcttttcagc
    16741 tgaccaaagt gaattgatag ctgaaatttc aataggagcc ggcatccatc ctaggaggga
    16801 gacgatgaag ggcagagctg ccatttgcca aggtgtcttc tcgacaaaat cagaactgta
    16861 ttctggatgc ttgaccgccg caatgataac tgcaagaaca gtcgcaatgg ttaaggcaga
    16921 cataatccat ttgaccatgc cgtctaaaag tttgtagcct ccaaagagta gcatagccca
    16981 aatgattgca acgagaatga gggaccactg agtaatgcta agtccaatca ttgggaaggc
    17041 actggcgatg atagctgagc acagaatggc aacaccagcc gtgttgacca tagccgaaaa
    17101 gacattgagg ataaagaaaa tccagagata gagttttcct ttttcggcat aaccttcaac
    17161 caaagtcttt ccagtatcag ctgtgtattc agcaccaaaa cggaaaaatg gatatttaaa
    17221 gacattggct aagatgacca agagaagtag agaccaaccg taagaaccgc cagcttgagt
    17281 tgaggataca atgtgggaac ctccaacagc ggcagttgcc attaggattc caggtcccat
    17341 tgcttttagc ttgcttgccc aggttgattg atttaaggaa atagcttgcg acatgataaa
    17401 tactcctttt tgaattttca gaataatctg tatatgtaat ctatattcta caaaaaattt
    17461 tggaaaaatg caagagtatt ttgaaaaaag atagaaaatt tacaaagaag atctgaaata
    17521 actgttatga tatagaggat tgaaactaga atagtacacc tctgcttcta aaacattgtt
    17581 agaaatcgat ttgactgtcc tgatcgattt atcctgttct tatttcattt cactatagta
    17641 aggtgcagca ggataaagaa aaaccgagaa gtttctttct cggatttgct tattatgagg
    17701 ttcctatttt ctatgctcca agagttgatt gatgtgatct acttttttag cctctctttt
    17761 atagagaata gcccagatga tgagatagac aatcgcaaac tcgataatga gctggaggta
    17821 gaaaatccag tggaaaggga accaaccagc aagagttgct agtgggacaa agccagccag
    17881 cataaggaag aaatgagtca gagtggcacg aagcatgctc cagtcacggc taaataagcg
    17941 gttgccaaag ttgaagagca taccgatggt tgcccagata agtgtgcagt agagcaagac
    18001 cagggcaccg tgaacctgat gctgatccat cacttggcct atgagagagt agggatttag
    18061 tggtgcgtag gtatttggtg cataaatgag tgaaaagagg atagagagga tgaggccgat
    18121 aagaacaccg gtagctgcat cgtgaaatac ttgttttttc atagttctaa tttctccttg
    18181 atggttttta gataacggcg tgaagagtag gtgaagcttt cgtttttcaa gaaaatttct
    18241 accagaccgt ttggcgtgag cttgaggtga gagatggaat cgatattgat gatttctgat
    18301 tgggaaattt ggataaaatt gcttggcaag agtttaagaa cctgatagag tcgcaaatca
    18361 atgctgtagg tctgactcgc ggtttctgct agaaccttcc gattctcgat atagaagcgc
    18421 tgaatcttgc caatctcaac tagatagacc tgatcatcga tttttccttt gattttttct
    18481 ctttggtcca gattttctgc gaactcgatg actttctgga ctttttcggt ttcttgaggt
    18541 gcttggacaa tcagcttttc ctcctcgtaa gtctcactaa tctgtagttc tactttcata
    18601 aatttctctc cttgtttttc atacaaaatt aatctcactt cttgtacacc tttattaata
    18661 ctcttcaaaa atctcttcaa accacgtcag cgtcgtcttg ccgtaggata gttgttgact
    18721 ttgtcagttt tatctacaac ctcaaaacag tgttttgagc tgtctgcgtc tagctttcta
    18781 gtttgctctt tgatttttat tgagtatacc actattttac tccctctggc aagggacttt
    18841 gtctatgtgg agggattggg ctcctatgtg gtggagtttt tctgttcttt ctgaaatatg
    18901 gtataatagc actaattaat ttctaggaaa atagatacag aaaggggctg aaagatgtct
    18961 catattattg aattgccaga gatgctggca aaccaaatcg cagctgggga ggtcattgaa
    19021 cgtcctgcca gtgtggttaa agagttggta gaaaatgcca ttgacgcggg ctctagtcag
    19081 attatcattg agattgagga agctggtctc aagaaggttc aaatcacgga taacggtcat
    19141 ggaattgccc acgatgaggt ggagttggcc ctgcgtcgcc atgcgaccag taagataaaa
    19201 aatcaagcag atctctttcg gattcggacg cttggttttc gtggtgaagc cttgccttct
    19261 attgcgtctg ttagtgtctt gactctgtta acggcggtgg atggtgctag tcatggaacc
    19321 aagttagtcg cgcgtggggg tgaagttgag gaagtcatcc cagcgactag tcctgtggga
    19381 accaaggttt gtgtggagga tctctttttc aacacgcctg cccgtctcaa gtatatgaag
    19441 agccagcaag cggagttgtc tcatatcatt gatattgtca accgtctggg cttggcccat
    19501 cctgagattt cttttagctt gattagtgat ggcaaggaaa tgacgcggac agcagggact
    19561 ggtcaattgc gccaagcaat cgcagggatt tacggtttgg tcagtgccaa gaagatgatt
    19621 gaaattgaga actctgacct agatttcgaa atttcaggtt ttgtgtcttt acctgagttg
    19681 actcgggcta accgcaatta tatcagcctc ttcatcaatg gccgttatat caagaacttc
    19741 ctgctcaatc gtgctatttt ggatggtttc ggaagcaagc ttatggttgg acgttttcca
    19801 ctggctgtca ttcacatcca tatcgaccct tatctagcgg atgtcaatgt gcatccaact
    19861 aagcaagagg tgcggatttc caaggaaaaa gaactgatga ccctggtttc agaagctatt
    19921 gcaaatagtc tcaaggaaca aaccttgatt ccagatgcct tggaaaatct tgccaaatcg
    19981 accgtgcgca atcgtgagaa ggtggagcaa actattctcc cactcaaaga aaatacgctc
    20041 tactatgaga aaactgagcc gtcaagacct agtcaaactg aagtagctga ttatcaggta
    20101 gaattgactg atgaagggca ggatttgacc ctgtttgcca aggaaacctt ggaccgattg
    20161 accaagccag caaaactgca ttttgcagag agaaagcctg ctaactacga ccagctagac
    20221 catccagagt tagatcttgc tagcatcgat aaggcttatg acaaactgga gcgagaagaa
    20281 gcatccagct tcccagagtt ggagtttttc ggacaaatgc acgggactta tctctttgcc
    20341 caagggcgag atggacttta catcatagat cagcacgctg ctcaggaacg ggtcaagtac
    20401 gaggagtacc gtgaaagcat tggcaatgtt gaccagagtc agcagcaact cctagtgccc
    20461 tatatctttg aatttcctgc ggatgatgcc ctgcgtctca aggaaagaat gcctctctta
    20521 gaggaagtgg gcgtctttct agcagagtac ggagaaaatc aatttattct acgtgaacat
    20581 cctatttgga tggcagaaga agagattgaa tcaggcatct atgagatgtg cgacatgctc
    20641 cttttgacca aggaagtttc tatcaagaaa taccgagcag agctggctat catgatgtct
    20701 tgcaagcgat ctatcaaggc caatcatcgt attgatgatc attcagctag acaactcctc
    20761 tatcagcttt ctcaatgtga caatccctat aactgtcctc acggacgtcc tgttttggtg
    20821 cattttacca agtcggatat ggaaaagatg ttccgacgta ttcaggaaaa tcacaccagt
    20881 ctccgtgagt tggggaaata ttaaaagtat aaaaaagtct gggaaaaatt ttcaaaatca
    20941 gaaaaacgca taaaatcagg tgttcaaaaa ccttgatttt atgcgtttta tcatggaaat
    21001 agttacttca tgttttccta attcttttcg aaactctttt taaaccacgt cagttttatc
    21061 agtaatctca aaacagtgtt ttgagctaat tttgccagtt ttgtctgtaa catcgaagtt
    21121 gtgttttacc actctgcgac tggtttccta gtttgctcta tgattttcac agagtattaa
    21181 attgcgattt tgccaagttt ctttattcgt ctaaaagtag agtctgttct atgcgtctaa
    21241 tgtacgaatt aggttgacca tttcaatagc tccttgtgca cactcagaac ccttatttcc
    21301 tgctttggta ccagctcgtt ctatggcttg ttcaattgta tctgtcgtta gcacaccaaa
    21361 cataacagga atttcgctat ttaaactgat ttgggcgatt cccttagata cctcgctaca
    21421 tacataatca taatgacttg tattccctct aatgacagct cccaagcaga taattgcatc
    21481 atatttttta ctttttgcca tttttgatgc aatcagtggt atttcaaaag ctcctggaac
    21541 ccaggctacc tctatatctt tctcgtttac attctctctt ttgagattat ctagtgctcc
    21601 agataataat tttgaagtta taaattcatt aaatctcgct acaacaatac ctattttaat
    21661 attgtttgct actaaattac cttcataagt gttcatttat ttttcctcca tatttaaaat
    21721 gtgacccatt cgatttttct ttgtttctaa ataaaaacta tcgtaaggat tggcttctat
    21781 ttcgattgat attctactgg aaatggtaat tccatatttt tctaactgtt caaccttgtc
    21841 aggattattt gtcagtaaat gaagtgactg aagtcccaga tctttaagca tttgtgctcc
    21901 aatatgatat tctcttaaat caccttcaaa gcctaatgca agattggcat caagcgtatc
    21961 catgccttga tcttgtaaat gataggcttt taatttattg ataagtccaa ttcctcgtcc
    22021 ctcctgtcgc aagtaaagta agacacccga accattctca acaatcattt tcatagcttt
    22081 atcgaattgc tgtccacaat cgcaacgtaa agagcctaaa acatctcctg ttaaacattc
    22141 ggagtggacc cgacataata cattggcttc atcctctatg tttcccataa taagagcaag
    22201 atgatgttcc ccatttagtt tatctatata gctaattgct ttgaaattac cgtatctagt
    22261 aggcatattg acagttgaaa ctcgttctat cagctgatca tatacttttc tatattcttg
    22321 taattctttg atggtaatta gtggaatgtt gtgtttcttc gagaactgaa ttaaatcatc
    22381 tgttctcatc attttgccat catgattcat tatttcacaa cataggccac actcttttag
    22441 tccagctaat tttaataaat caacagttgc ttctgtgtgt ccatttcttt ctagaacacc
    22501 accttttttt gcaattaaag gaaacatgtg tcctggcctg cgaaaatcag agggtgttat
    22561 atcttcagct acacacatac gtgcggtcag tcctctttcc tcggcagaaa tacctgtggt
    22621 cgtttcttta taatcaattg aaactgtaaa agcagtctta tgattatctg tattgttttc
    22681 aaccataggt gaaagcatta attgattagc taaactttcg ctcataggca tacaaattaa
    22741 tcctttggca taagtagcca taaaattaac attttctgtt gtagctgctt gtgcagaaca
    22801 aattaagtct ccttcatttt ctctatcctt gtcgtctata acaagaacaa gtcgtccctt
    22861 ctgcaatgct tctaatgttt cttgtatttt tcgatattcc attgactgat tatcctttct
    22921 gctaaaatcc attttgatat aatagttcct tagatatttc tgattttgga gagttatcca
    22981 tcagtttttg cacatattta cctaagatat cattttcaag atttactgta ctcccgactt
    23041 gtttactttt aagaatggtt tgtttcaagg tatgagggat aacagatact gaaaagttta
    23101 ctttggagac tttagcgaca gtcagactaa tgccgtcaat tgtaatagat cctttttcaa
    23161 ctattaaatc taaaatttct ttttgtgtgt tgatttgata ccatacagca ttatcatctt
    23221 tttttattga cgagattttt cctgtaccat caatgtgtcc tgtaacgacg tgacccccaa
    23281 gtcgaccgtt gatagataag gctctttcta gattcacctc acttccatgt tttaatagag
    23341 taagagctgt tcgactccat gtttcattca ttacatcaac tgtaaaggat tgatgattga
    23401 aatgagtaac tgtaagacag ataccattta ctgctatact atcgcctaaa tggatatccg
    23461 ttaatatttt tgaggcttta attgatagtt tacaattacg agagtctttc tgtattcttt
    23521 caacttttcc gatttcttca attattcctg tgaacatgga taaatcactt cactttctat
    23581 gagatagtca tttcctattt gagaaaaagc ataaggtttc aatctaatag cgtcatttgg
    23641 caaagaaatg ccttcacctc cgacaggaaa cttggcactg cctccaaaaa tttttggtgc
    23701 aatatatatt ttcagctcat caacaatttg ttgttccaaa gcactccaat tcattagact
    23761 gcccccttct agaactaggc tatcaatctg catgtttcct agatgttgca ttaaactcga
    23821 taagtctata tgattgcctt ttttctttat ggaaagtatt tcacagccat gattttgata
    23881 tagcttcatt ttatttttgt cttcagagga agtggcaatg taagttttaa tatcatttgc
    23941 tgtttttacg attttagagg taagaggagt tcgtaaatgt gtatcgcata tgatacggat
    24001 aggatttttc ccttcctcca atctacatgt cagcaaagga tcgtcttgaa taacagtatt
    24061 gactcccacc ataattgcac taacatggtg tcgtaactga tgcacatgct ttcttgcttc
    24121 ttcttcagta atccatttgg attgatttgt tttagtggct atttttccat ccattgacat
    24181 tgcatatttc ataaaaacat agggtacatg ctgggtaata tactttctaa aactttttat
    24241 taagttaaga cactcatttt ctaaaattcc aacagtaact tgaagattat tttcctcaag
    24301 tatctttact ccttttccag atacaatagg attacagtct aggcttccaa tgactactct
    24361 tgtaatacca ctatcgatta tagcatctat acagggaggt gttttcccga agtgacaaca
    24421 gggttcaagt gttacataaa gcgtcgctcc gacaggggat tctctacagt ttttaagagc
    24481 atttctctca gcatgtgggc caccaaaaaa ctcatgataa ccttgtccga tgatgtgatt
    24541 atcttttaca ataactgcgc cgaccatagg attgggattg acgtaaccag cccctttttg
    24601 tgccagtttt attgctaatt tcatatattt tgaatcgctc atctcactac ctccaaaaaa
    24661 aatatacctt gaatagggga ctactcaagg catacaaaag aaaacttatg cgattaacaa
    24721 aaatactctg aaatgacaag taatcatttc agagcacgca aaaagcacaa atatactttt
    24781 atcttctttc atccagacta tactgtcggc tttggaattt caccaaatca tgcctttcgg
    24841 ctcgtgggct ataccaccgg tagggaatta caccctgccc tgaagatagt tattcaatta
    24901 cagatgatta tagtacttaa ttttgaatat gtcaacagat aaataccgat tgtttttgat
    24961 atactgtatt tgtgataatc gattctcgct cctcggataa agaaaatatg atatactaga
    25021 taaacgaaat aagagagaag gaatactatg tacgcatatt taaaaggaat cattaccaaa
    25081 attactgcca aatacattgt tcttgaaacc aatggtattg gttatatcct gcatgtggcc
    25141 aatccttatg cctattcagg tcaggttaat caggaggatc agatttatgt gcatcaggtt
    25201 gtgcgtgagg acgcccattt gctttatgga tttcgctcag aggatgagaa aaagctcttt
    25261 cttagtctga tttcggtctc tgggattggt cctgtatcag ctcttgctat tatcgctgct
    25321 gatgacaatg ctggcttggt tcaagccatt gaaaccaaga acatcaccta cttgaccaag
    25381 ttccctaaaa ttggcaagaa aacagcccag cagatggtgc tggacttgga aggcaaggta
    25441 gtagttgcag gagatgacct tcctgccaag gtcgcagtgc aagcaagtgc tgaaaaccaa
    25501 gaattggaag aagctatgga agccatgttg gctctgggct acaaggcaac agagctcaag
    25561 aaaatcaaga aattctttga aggaacgaca gatacagctg agaactatat caagtcggcc
    25621 cttaaaatgt tggtcaaata ggagcagaga atgacaaaac gttgttcgtg ggtcaagatg
    25681 acaaacccgc tctacatcgc ctatcatgat gaggagtggg gtcagcccct ccatgatgac
    25741 caagcattgt ttgagttgtt gtgtatggaa acctatcagg caggcctgtc ttgggaaacg
    25801 gtactcaaca aacgccaagc tttccgagaa gcctttcata gctatcaaat tcactcagtc
    25861 gcagagatga ctgagactga attggaagcc atgctggaga atccagctat cattcgaaat
    25921 agagccaagc tttttgctac acgcgctaac gcccaagtcc ttctacagtt acaggcagag
    25981 tacggctctt ttgatgccta tctttggtct tttgttgagg ggaaaattgt cgttaacgat
    26041 gttcctgatt atcgccaagc gccagctaaa acacccttat ctgagaaatt agccaaagat
    26101 ctcaaaaaac gaggcttcaa gttcacaggc ccagtcgccg tattgtcttt tctacaggct
    26161 gcagggctag ttgatgacca cgagaatgat tgtgagtgga aaggtcttaa atgatgtcta
    26221 acaaaaataa gggaattctg atttttgcga ttctctatac agtcctcttt gtgtttgatg
    26281 gcgttaaatt gctggcttct ttaatgccat ctgtcattgc aaattatctt gtttatgtag
    26341 ttttagcttt atatggctcc ttcttgttca aggatagatt gatccaacaa tggaaggaga
    26401 ttagaaagac taaaagaaaa ttcttctttg gcgtcttaac aggatggctc tttctcattc
    26461 tgatgactgt tgtctttgaa tttgtatcag agatgttgaa gcagtttgtg ggaccagatg
    26521 gacaaggtct aaatcagtct aatattcaaa gtacctttca agaacaacca ctactgatag
    26581 ctgtttttgc ttgtgtcatt ggacctctgg tagaagaatt atttttccgt caggtcttat
    26641 tgcattactt gcaggaacgg ttgccaggtt tactaagcat tattctggta ggacttgttt
    26701 ttgctctgac tcatatgcac agtttggctc tatcagagtg gattggtgca gttggttact
    26761 taggtggagg ccttgccttt tctattattt atgtgaaaga aaaagagaat atctactatc
    26821 ccctacttgt tcacatgtta agcaacagcc tctccttaat cattttagct atcagtatag
    26881 taaaatgaaa taagaacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt
    26941 tttagaagta gaggtgtact attctagttt caatttgctg taatatgtga tgaaaatacc
    27001 agtaatgata ccgagaaaaa agctgagaaa cttttcccag ctttatttgt tatagtcaaa
    27061 gcgaatgact tgttcctgtg catctacatg agcatggacc ccaaagggta caattgctct
    27121 tggagttgcg tggccgacat tcagattata gacaatcggg atattgctgt caatgatatc
    27181 caatagtgcc tctttatagt cgtcatggaa agtttcatcc ataggttttc cgaccaagag
    27241 tccattgatg accgcgaata tgccagtgtc ctttaaagtt agcaacatct ttttgaagtc
    27301 ttctggctta ggcttttctt cgcttgtttc gagcaagagg attttccctt cccagtctga
    27361 caagtcaggg aaaagtttgt atttttggca gagttccgtg ctatctgcgt atcgagagtt
    27421 gtcaaagata tcgtagaggg attcgaggca accaccgagg atttttccct cgaactgagc
    27481 acttccttgc aacaagtcaa aacctgtatt tgtatgactg acacgaggtg ttcccagggc
    27541 cgtgggacta aaatcagttc gttcctcata ccaaacgtca ctagggcgga tttctgaaat
    27601 tcttcccgtc ttaatcaatt ctttaaagta gtgaaggcta taggctagca tttctttgtc
    27661 taattcacaa atgtctgcta aaaaggattg accataaaaa gtcttgattc ctaatttatg
    27721 caacatgagg tggttcatgg ttgtatccga gaagccaaga aaaatttttt gcttgataac
    27781 cttttggagt tggtcatttt caaaaagata aggtagcaag cgataggtat cgtctccacc
    27841 gatggcacat aggatcatgt cgatgctatc atcagaaaag gcatgaatca aatcctctgc
    27901 acgagcttca ggatggtcct tgataaagtc taatcctttt aacgaatggg acaaaaagat
    27961 gggattgagt cccagatcct tgagacgttg gatacccaag tccacttcgt gtttgacaaa
    28021 atcctctccg ataatgccac tagataaact aacaatacca atagtagaaa ccatatctca
    28081 tcctcctaga aatagattga gcctatttta tcacaaaata taagagaaag ctatgtggga
    28141 tttcagaaga atacttttaa tactcttcga aaatctcttc aaaccacgtc agttttatct
    28201 gcaacctcaa agctgtgctt tgagcaacct gcagctagct tcctagtttg ctctttgatt
    28261 ttcattgaat ataactcaaa aaagtaagtg aaaaagcaac cgcacctgca gttgctttta
    28321 tcgttttctt attcttgata gcgtgttggt cctgcacttg cactacggat attcaagcgt
    28381 ttcttgagat agaagcgaag ggctaggagg gctgctccaa taatagctag tggcaatgga
    28441 tccagtactg ggttaaggct agctggtagg aagcttgttg caaagaagac aagcaaccaa
    28501 aggaacatag aagctaggat aactagtaca gatttccaga aaggtggacg ttgactgcga
    28561 tccatatctg atccatagta ttggtaaaca aagtagtaca tcaagtagaa ggcaaatcca
    28621 ccaaccagtc caactaatag aagagtaatc aatccatagc cgaaagcttg gtctgccgca
    28681 aagaaggttg tgagggcgct gacaagggca aagaggctag tgatgaaaag agctgagtcc
    28741 ataatcatca gttttgggtc atcattttct tttggatgct ctttttcgta ctgctctttg
    28801 acagtgaagc tatgagccca atgagttggt gcgccgtata gggaacgggc agttgtacct
    28861 ttagattgct cctcaaggat ttggggaatg acttcctcaa aaatagtctg gatttcagcg
    28921 tctgttttcc catctttgat gaattgttgg gtagcaatgt ggacaaactc ttggtttttc
    28981 ttagttaatt tttgtagatc aatttgagac ataggaactc ctcttagaac cattttttat
    29041 ggatgagata gagagtgagc gagacactca tagcaaaggc gataaagacg attaaccaga
    29101 aggcatttgg ctctccgttt agggggattt cattatcctt aaagttcatc ccgtaggcag
    29161 aaaagaccat ggttgggatg gacatgacga tggtcacaag ggccaaggtt ttcatgatgt
    29221 tgttctggtt gttagaaatg atagaggcaa aggtctctgt catagaatgc aagacgtttc
    29281 cataaatatc tgccatctcg atggcctgtt gggtttcaat cagggtgtct tcaagcaggt
    29341 cttcgtcctc aaggtatttc ttgatattgc tggttgaact ggtcaatttc ttaatcacgc
    29401 gctcatttgt tttgagggag gccttgaaat agacgatagt tttttccaat tccatgagct
    29461 caatcaattc ttcattacga gttgattgat gcagttgact ttcgatttgt tcactcttgc
    29521 ggtcgattga acgaagggct gttaggtaaa gctctgcatt gcgataaaga atttgaaaga
    29581 taaaacgtga acgcatgaag gtatagaaat tacgcaatcg acggttgata aagacatcaa
    29641 ggacaggtag tggttccaaa cacgtagtga taatggtttc ctcagtgatg ataataccaa
    29701 gcgggatggt tacgtagtag gtgcggttat ttctttcctc cgtgaccggc acgtctacga
    29761 taatcagggt atactcgtct tcaatggtaa tacgagacat ttcttccgca tcgagcggtg
    29821 ctcgaaggtc ggcaatatca atatcgaagg tgttggcgat ttcgagtgat tcattttgag
    29881 tcggattgac gagattgatc caagtacccg gttcaagcgt atcgatctct ttaaattcag
    29941 ttgttgtaga gagaaaaact tgtttcatat cccttatcct ttctcatttt tcagattttt
    30001 tcacactgta ctattatact acaaaatcgg ccttttgggt aatagaatct cacttttttt
    30061 ggtataatgg taagcaataa tggactagaa agaacaaaga tgcaagataa aattgtcatt
    30121 catggggcgc gtgcccataa tttaaaaaat attgatgtgg agattccgcg agacaagttg
    30181 gttgtcgtga ctggcttgtc aggttcaggg aagtccagtc tggcttttga tactctctat
    30241 gcggagggac aacgtcgcta tgtagagagt ttgtctgcct acgctcgtca gttcttggga
    30301 aatatggaaa aaccagatgt agatgccatt gatggtctca gcccagctat ttccatcgac
    30361 cagaaaacga ctagtaaaaa tcctcgctcg acggtgggaa cgacgactga aatcaatgac
    30421 tatctgcgtc tcctctacgc acgtgtgggg acgccttact gtatcaacgg acatggagct
    30481 atcaatgctt cttctgtgga gcaaatcgtt gataaggttt tggagttacc tgaacgccag
    30541 cgcttgcaga tcttggctcc tgtcatccgc aagaaaaaag gccaacataa gagtgttatc
    30601 gagaaggttc agaaagacgg gtatgttcgt gttcgtgtgg atggggaagt ctatgatgtg
    30661 accgaagtgc cagagttgtc taagagcaag caacacaata tcgatgtcgt ggttgaccgt
    30721 attgttatca aggagggcat tcgtagccgt ctctttgatt ccattgaagc tgcccttcgt
    30781 atcgcagaag gttatgtgat tatcgacacc atggacgact ctgagttgtt gttctctgag
    30841 cattatgcct gtccagtttg tggatttact gtcccagagt tagaacctcg tctcttctct
    30901 ttcaatgctc cctttggctc ttgtagtgag tgtgacggct tggggattaa gctggaggtg
    30961 gatactgatt tggtagtgcc agatgccagc aaaaccttac atgagggagc gctggcacct
    31021 tggaatccta tctcatccaa ctactatcca aacatgttag agcaggccat gaaagtcttt
    31081 ggagtggcta tggataagcc ttttgaggac ctgtcagaag aagataagaa cttgattctc
    31141 tacggctcag atggtaagga attccatttc cattatgaaa atgaatttgg tggtgtgcgc
    31201 gatatcgaca ttccgtttga gggagttatc aataatatca aacgtcgtta ccatgaaacc
    31261 aatagcgatt acactcgcac tcagatgcgt ctctacatga atgagctgac ctgcggaacc
    31321 tgtcaaggct atcgtctcaa tgaccaggcc ttgtctgtcc gtgtgggcgg ccagcaaggg
    31381 ccacatattg gagaaatctc agacctgtct atcgctgacc acttggactt ggtgagccag
    31441 ttaactttgt ctgaaaatga agccatcatt gctcgtccca ttctcaagga aatcaaggat
    31501 cgtttgacct tccttaataa tgtgggtctt aactatctaa ccctgtcacg ttcagcagga
    31561 accctttcag gtggggaaag tcagcgcatt cgtttggcaa cccagattgg ttccaaccta
    31621 tcaggtgtcc tctatatcct agacgagccg tcaatcggtc ttcaccagag ggacaatgac
    31681 cgcctgattg ccagtctgaa aaagatgcgt gacttgggca atactcttat cgtggtggaa
    31741 cacgacgaag ataccatgcg tgaagctgat tatctgattg acgttggtcc tggtgccggt
    31801 gtttttggtg gtgagattgt tgcagcaggt acacctaagc aagtggctcg caacagcaag
    31861 tctatcacag gtcagtactt gtcaggcaaa cgtgtcatcc cagtaccaga agagcgccgt
    31921 gtcggaaatg gtcgttttat tgaagtgaca ggagcgcgtg agaacaactt gcaaaatgtc
    31981 acggctcgct ttccactagg aaaatttatc gcagtgacag gtgtgtcggg ttcagggaaa
    32041 tcgactctaa tcaacagcat tctcaaaaaa gccattgccc agaagctcaa ccgtaattca
    32101 gacaaacctg gtaagtttaa gactattaca gggattgagc atgtcgaccg tttgattgat
    32161 attgaccaga gccctatcgg acggacgccg aggtctaacc cagctaccta tacaggagtt
    32221 tttgacgata tacgtgacct ttttgctcag acaaatgaag ccaagattcg aggctacaag
    32281 aaggggcgct tcagtttcaa cgtcaaggga ggtcgctgtg aagcttgctc aggtgacggg
    32341 attatcaaga ttgagatgca cttcttgcca gatgtttatg tggcttgtga agtttgccac
    32401 ggaacccgct ataacagtga aaccctagaa gttcactaca aggaaaagaa tatttcgcag
    32461 gtcttggata tgacggtcaa cgatgcggta gaatttttcc aacacattcc gaaaattcaa
    32521 cgcaaactcc agaccatcaa ggatgtaggt ctaggctatg tgaccttggg acagccagct
    32581 accacccttt ctgggggaga agcccaacgt atgaagttag ctagcgaact ccacaaacgc
    32641 tcgacaggaa aatctttcta cattctggat gagccgacga cagggcttca tacagaggac
    32701 attgctcgcc tgctcaaggt tttagctcgc tttgtagacg ccggcaatac agtcctcgtc
    32761 atcgagcaca atctggatgt tatcaagacg gcagaccata tcattgactt gggacctgag
    32821 ggcggtgtcg gtggtggaac catcatcgta acaggaactc cagaagaagt agctgccaac
    32881 gaagccagct atacaggaca ctatttgaaa ggaaagttac atcatgaata aacgcgtaca
    32941 agcatttcta gctaaaatgc aagaaaaaga actagatggt atcatcatca acaatcttaa
    33001 aaacgtctat tatttgactg gtttttgggg ctcaaacgga acagtcttta tcagccgtga
    33061 ccgtcaggtc ttagtgacag actctcgcta tatcatcgca gctaagcaag aaaccagtgg
    33121 ttttgagatt gtggctgatc gtgatgaatt ggctgtcatt gcaggaattg ttaaggacat
    33181 gaacttgact cgtatcggtt ttgaagatga gatttcagtg tcttattacc accgtatgca
    33241 ggcagctttt gcaggtttga acttgcttcc acaaactcag tttgtggaag gtcttcgtat
    33301 gattaaggat gaggcagaga ttgcagcgat tcgcaaggct tgttctatct cagaccaagc
    33361 tttccgcgat gcgcttgact ttatcaaacc aggaaaaact gaaattgaga ttgccaactt
    33421 ccttgatttc cgcatgcgtg agttgggagc atctggctta tcttttgata cgatcctagc
    33481 tagcggtatc aattcttcta aaccccatgc ccatccaatg cacaaaccag tggagttggg
    33541 agaagccatt accatggact tcggctgtct ctatgaccac tatgtcagtg atatgacccg
    33601 gactatctat ctagggcatg ttagcgatga gcaggcagag atttacaata cggttctaaa
    33661 agctaaccaa gccttgattg accaagctaa ggcaggctta ggtttccgtg actttgacaa
    33721 aatccctcgt gatattatca ttgaggcagg ttatggtgac tactttactc acggcattgg
    33781 ccacggtatt ggtctggata tccatgagga accctacttt agtcagactt ctacagaaac
    33841 tattaagaca ggtatggcct tgaccgatga accaggtatc tatatcgaag gcaaatatgg
    33901 cgttcgtatc gaggatgata tcctgattac agagacaggt tgtgaattat tgaccctagc
    33961 tccaaaagag ttgatagtca tttagttgtt tgtcaagaaa aaagtacgaa aatgacgaaa
    34021 aaagtcaaaa aaaattaaaa ataggtcgca agtcggatgt tttttatggt ataatagact
    34081 aaactgatag taacatgtag cgaaaggggt aggtacatga ttaaaattta tacagtctca
    34141 agttgtacta gttgtaaaaa agcaaaaacc tggctcaatg cccaccagtt aagttataaa
    34201 gaacaaaacc ttggtaaaga aggaattacg agagaagaat tactggatat tctaaccaaa
    34261 acagataacg gaatagccag catcgtttcg tctaaaaatc gctatgccaa agcccttgga
    34321 gtggatattg aagatttgag tgtcaatgaa gttctcaatc tgattatgga aacaccgaga
    34381 attttaaaga gcccaatcct tgtagatgaa aaacgcctgc aagttggcta caaggaagac
    34441 gatattcgtg ccttcctacc acgctctgtc cgtaatgtag aaaatgcaga agcacgtttg
    34501 cgtgcagctc tataaacatc aaggctggga gcacttccca gtcttattct attttaattt
    34561 caaaaagaaa gaagaaagaa atgaaaaaaa tagttcttgt tagtctagct ttcctttttg
    34621 tcctggttgg ttgcggacag aaaaaagaaa ctggaccagc tacaaaaaca gaaaaagata
    34681 cgcttcagtc ggcattgcca gttattgaaa atgctgagaa gaatacagtt gtaactaaga
    34741 ctttggtctt gcccaagtca gatgatggta gccagcagac acaaacaatt acttacaaag
    34801 acaagacttt tttgagtcta gctatccaac aaaaacgtcc agtctctgat gagttgaaga
    34861 cttatattga ccaacatgga gtggaggaaa ctcaaaaagc tcttcttgaa gcggaggaga
    34921 aggataagtc tatcattgaa gctcgtaaat tggcaggttt caaacttgaa acaaaactat
    34981 tgagcgcaac ggaacttcaa acaacgacta gttttgattt tcaagttctg gatgtcaaga
    35041 aggcttccca gttggaacat ctgaagaata ttggtttgga aaatcttttg aaaaatgaac
    35101 caagcaaata tatttcagat agattggcaa atggcgcgac agaacaatag aaaatccaaa
    35161 taaatagact gcctgaattg tagaaggtaa ggaattatgc tataatggta ctattctaag
    35221 gaaagagggt gttagaatgg gatttactga agaaacagta cgttttaaat tggacgattc
    35281 caataaaaaa gaaattagcg aaactttgac agatgtttat gcttcgttga acgataaggg
    35341 ttacaaccca attaaccaaa tcgtaggtta cgtattgagt ggagaccctg cctacgttcc
    35401 tcgttataat aatgcacgaa atcaaatccg taagtatgag cgtgatgaaa tcgttgagga
    35461 attggttcgc tactacctca aaggacaagg agtcgatcta taacctatga gaattatggg
    35521 attggacgtc ggttcaaaaa cggtaggggt ggcgattagc gatccgcttg gttttacagc
    35581 tcaagggctt gaaatcatcc agataaatga agaacaaggc caatttggtt ttgaccgcgt
    35641 taaggaattg gttgatactt acaaggtgga acgatttgta gtgggcttgc ctaaaaacat
    35701 gaacaataca agtggaccgc gcgtagaagc tagtcaagcc tacggagcaa agctagaaga
    35761 gttttttggt ttaccagtag actatcagga tgaacgcttg acaacagtgg ctgctgagcg
    35821 catgttgatt gaacaagcag atatcagtcg caataagcgc aagaaagtca ttgataagtt
    35881 agcagctcag ctgattttac aaaattattt agatagaaaa ttttaatata aaggagaggc
    35941 tatgtcacac gatcataacc acgaccacga agaacgtgaa ctaatcacac tagtagatga
    36001 gcaaggaaat gaaaccttgt ttgaaatcct tttgacaatt gatggaaaag aagaatttgg
    36061 taaaaactat gttcttctag taccagttaa cgcagaagaa gacgaagacg gacaagttga
    36121 aatccaagct tactcattca ttgaaaatga agatggaaca gaaggcgaat tgcaaccaat
    36181 cccagaagac tcagaagacg aatggaacat gattgaagaa gtcttcaaca gctttatgga
    36241 ggagtgatac agtctgggag actgtatcag cccaactcca gaaatgggga agagttggaa
    36301 cgtccagtga acatttttac cccaactctt agaaattgag aagagttgga acatctagtg
    36361 gatgttttta gcccatattc acattcatag tagctagtta ttagctaacc aggtaagagg
    36421 tcgggacgaa aatcctggcc ttgtttttat tagtaaccat actttaatgc gataattgat
    36481 tggacttttg tcaaggagat gtgtatgttt gaagtagaag aatggctcca tagtcggatt
    36541 ggtttgaatt ttcgatcagg tttgggccga atgcagcaag cggtggattt gttaggaaat
    36601 cctgagcagt cttaccctat tatccacgta acagggacta atgggaaagg atctaccatt
    36661 gcttttatga gggaattatt tatggggcat ggcaaaaaag ttgcgacctt tacctcccct
    36721 catatcgtct ctatcaatga ccgaatctgc attaatgggc aacctatagc agacgcagac
    36781 tttatccgtt tgactgatca ggtcaaggag atggagaaaa cgcttctgca aactcctgcc
    36841 cagttgtcct tttttgaatt gctgacctta gttgcttttc tttattttag ggagcaagag
    36901 gtggatttgg ttttattaga agtgggaatt ggtggcttac ttgacacgac caatgtggta
    36961 actggagagc ttgctgtcat cacctccatt gggcttgacc atcaagaaac cttgggtgat
    37021 agtctagaag caattgcaga gcagaaagct ggtattttca aggctggtaa aaaggcagtg
    37081 attgcgaaat tgcctccaga agctagactt gtctgtcaga aaaaagccga atctttagct
    37141 gttgaccttt atcaggcagg tcaagatttt tcagtgctga atggtgattt ttcaagctct
    37201 ttactaaatc tttcacagct gaacataggc ttagaaggag cctatcagca ggagaatgcg
    37261 gccttggcgt tgcaaacttt tcttcttttt atgggagaaa gaaaggaagc tattgctgaa
    37321 caggctgtaa gaaaggcctt ggaacagacc cattgggctg gtcgcttgga gcgtattcgc
    37381 ccacagattt acttggatgg tgctcataac ctccctgcct tgactcgctt ggttgagttt
    37441 atcaaagaaa aagagcagga aggctatcga cctcaaatcc tctttggatc cttgaaacgt
    37501 aaggattatc aagggatgtt gggttatctg actgaaaatt tacctcaagt ggaactcaaa
    37561 atgaccggct ttgactatca agggtctttg gacgagacag atgtaacagg ttaccatgta
    37621 attccctctt accgagaatt tatcagcagt ttcgaagaaa gggcagacgc tcaagacttg
    37681 ctgttcgtta cagggtctct ctattttatc tcagaagtac ggggctacct gctggaccgt
    37741 gagcagataa attgaccccc ttttttgaac ttgttatact aggggaattg ctggttagag
    37801 aaaatttctc taaattggta gcagaaagga aattcatcat gaaattaaaa agattcacac
    37861 tttctcttgc ttctctagca agttttagtc tcttagtagc ttgttcacaa agagctcaac
    37921 aggttcaaca gcctgttgct cagcaggtcc aacagcctgc tcaacagaat accaatactg
    37981 caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca aaaccaacct gttgctcaac
    38041 cgaccgatat tgatgggact tatactggtc aggatgacgg agaccgtatc actttagtgg
    38101 taactggaac gactggtaca tggactgagc tcgaatctga cggggatcag aaagtcaaac
    38161 aggttacatt tgattcagca aatcaacgca tgattattgg cgatgatgtc aaaatttaca
    38221 ctgtaaacgg taatcaaatc gtcgtagatg atatggatag agacccatcg gaccaaatcg
    38281 ttttaactaa ataagactat gcaagtagaa agggctggat ttttcagcct ttttactttt
    38341 acctagaaaa taatcataaa tacttgcctt ctaccgaata cctgcgttat actagtatca
    38401 attacttgtt tttagcattc aaactatcaa ggaggggata tgaaatatag gaaatttcaa
    38461 ttattgatgt ccaagtatgg ctttagtctt tcgattatgc tacttgaact ttgtcttgtt
    38521 tttggtctct ttctttattt aggacgcatg gctcccattt tatggattac tgtcctcatt
    38581 ctactgagta tcatcacaat catttcgata gtcaaccgta atacgactcc tgagaataag
    38641 gtaacctggt tgttagtagc ctttgtgcca gtatttggtc ccttgctcta tctgatgttt
    38701 ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga agaagctagg ctctatgcat
    38761 ttccaagaag caaatagcca gctactaaaa gagaaattaa aagaaagtga caaggcagct
    38821 tatggagtca tcaagtcctt attgagtatg gataccaatg ctgacatcta tgatcaaact
    38881 gcctctacat tttttcctaa cggagaagct atgtggaaaa agatggtaga agatcttaaa
    38941 aaggctgaga aatttatttt cttggaatat tacattatag aagaaggttt gatgtggaat
    39001 cgcatactag atatactaga gcaaaaggta gctcagggtg tagaggttaa gatgctctat
    39061 gatgatatcg gctgtatggc tactttaaca ggagattatg cacatcgact tcgtcagctg
    39121 ggcatcgagg cccataaatt caataaagtt attcctcgtt tgacagtggc ttataataac
    39181 agagatcata gaaaaatatt gattgttgat ggtcagatag cctatactgg tggggtcaat
    39241 ctggcagatg agtacattaa ccacgtcgag agatttggtt attggaagga tagtggaatt
    39301 cgcttagacg gactagcagt aaaagccctg acacgcttat ttttgaccac ttggtacatt
    39361 aatcgaggag aaattagtga ttttgatcaa tatcatttag aaaatcattc tatcccgagt
    39421 gacggtttaa ccattccata cggaagtgga cccaagccaa tttttcgagc gcaggtaggg
    39481 aaaaaagttt atcagagttt aatcaatcaa gcaacagaat cggtctatat tacgacacct
    39541 tatttgatta tagattatga tttaacagag acaatcaaaa atgcagctat gagaggggtc
    39601 gatgttcgaa ttatcacccc ttacatacca gataagaagt tcattcagtt agtcacgaga
    39661 ggagcttatc ccgaccttct ttctgctggt gttcggattt atgagtatag tccaggtttt
    39721 attcatagta agcagatgtt ggtagacgaa gattttgcgg tggtggggac aatcaatctc
    39781 gactaccgga gcttggtaca ccattatgaa aatgcagtct tactctataa aactccttct
    39841 ataagggaaa tcgcccgaga ttttcgaaat atatttgcag attctcagga agtctatcct
    39901 cattctatca aaacgagctg gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc
    39961 cctatcttat aagaaatcta gaacttagga tacaaaccag acctattttt cttgtctttt
    40021 actaatagaa agaaagagga ggggaattca tgctaactaa ggaagaagtg aatgatatga
    40081 ttgagttcaa acagacacat ttacaccgct ttcgggaaat tgaaaccttt gtgaaactat
    40141 gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc taggactttt tgatatacta
    40201 agacagataa attgaacagg agaaacgata tgagtgtgta tggtagagta gaagaagttc
    40261 ataaggagaa tcgtgaacct ctagaatacc aaatcgaaca agaatcgcat catcgtgaat
    40321 caagtcgtct tcctttggtg aaaattttac tatggagtac gcttgtaacg gggataactc
    40381 taggagtacc gctattactc gacttaatga gtgcacaaga agtgcaggat ttttatgcag
    40441 gttgggggct tcatcagaca gggaagattt acagcgacta ttatggaagt caaggtttgc
    40501 tttattattt gctgacttac gtgagtcagg gcggattttt ctttgccatc tttgagtggt
    40561 tagccttggt agcaggagga tttttccttt ttagatcagc ggacaccttg acagagcaag
    40621 gagaccaagc tggacatctg gtgactattt tttacatgct agttacaggt cttgcttttg
    40681 gtggaggcta tgcgactctt ttagcgcttc ctttcttatt cgcagccttt agtttagttg
    40741 cggcttacct aagcaatcca agccatgata agggatttgt acggattggg ctagctttgg
    40801 caggcggatt tttctttgct cccttatcat cgctcctgtt tattgctgta gtgagtttag
    40861 gcttgttggt ctttaacctt gggcatagac gctttgcgca tgggttttat cagtttcttg
    40921 cagtggcttt aggtttttca cttgtctttt atccaactgc ctactatagt gctgcaacag
    40981 gaagttttgg ggatgcgatt agtggtattc gttatcctat tgacagtatt cgctttgatt
    41041 ttacttctaa aattttagag aatatgtttt tttatggctt gttgtccctt ggtttgggat
    41101 ttgtggtttg tatcttttta ggtctctttc aatccaaacc ctttaaacta tacgtcattt
    41161 cggttcctgc aagtcttgtg gtaattttag gtttgatttt acttttcttt tcacaagagc
    41221 ctctgcacgc ttcttatttg atggtcgtct tccctgtttt cctactttta ttggtaacca
    41281 atattaagag tcaacagagg gggcgtagtg ctagaagaag ccgaagagaa acgccagtta
    41341 gcctatggag tcgtttcttc aaaggaaatc tatatctgct agtttttggg tttgtctatc
    41401 ttttgtctgt tccttttttg atgaagtttg tcctttatcc agtaccttat caagaacgta
    41461 atcgtcttgc tgatttggta aaagaggaga caaatacgga agatgctatc tatgcatggg
    41521 atgatactgc gactctttat cgtaagagtg agcgcttgtc gccatcggcg attttgtccc
    41581 cgttgcacta tacagcaact gaggaaaatc gtaataagtt acttaatgac ttgaaagaaa
    41641 aacaacctaa ggtgattgtg gtaaatgaca aggtggtagt ctggtctgaa gtggaaacac
    41701 ttttaaaaga aaattaccaa caagtaaaga ctgattactc agagtttaaa gtctataaaa
    41761 ttaaataacc aaatcaatat cttgtgtatt tttaaaaatt ttaggatttt taacacaaga
    41821 tattgatttt tctttttaga gtggtataat actttttaga aagaacattt tagaaaagag
    41881 catgcgtatg attgcactag aagaaaaaat tacaattttg ccaactctct tcgtcgagaa
    41941 acgagatggg agacgtgttg tatttgatgt ggacaagatt gacaaggctc tccacaaggc
    42001 ggctgacaag gttatgaatg tgacacccct ggttgaaaaa cgcctcaatg atctgactga
    42061 gcgaattatt acagaaattc atagtcgctt tccacaggga attaagattt acgaaattca
    42121 aaatatcgta gaacatgaac tccttgaagc caaagaatat gcgctggctg aggagtatat
    42181 tacttatcgg acacagaggg attttgagcg ctcaaaagcg acggatatca actttagtat
    42241 tcataaactt ctcaacaaag accagacagt tgtcaatgaa aatgctaata aagacagtga
    42301 tgtctttaac actcagcgtg atttgacagc agggattgtt gggaaatcaa tcggactgca
    42361 aatgcttcct aagcacgtag ccaatgccca ccaaaagggg gatatccact atcacgattt
    42421 ggactacagt ccctataccc ctatgaccaa ctgctgtttg attgatttta agggtatgtt
    42481 ggaaaatggt tttaagattg gaaatgcaga ggtagagagt cccaagtcta tccagactgc
    42541 gacagcacag atttctcaaa tcattgccaa cgttgcttct agccagtacg gtggctgttc
    42601 agctgaccgt atcgatgaaa ttttggcgcc ttatgcagag aagaattatc aaaaacatct
    42661 caaagatgca gaagagtggg tattgcctga aaaacgggaa gattacgctt ggaagaaaac
    42721 gcaaaaggac atctacgatg ccatgcaatc tcttgagtat gaaatcaata ctctcttcac
    42781 ttcaaatgga caaacacctt ttacttcgtt aggttttggt ctgggaacca gtcgttttga
    42841 acgagaaatt caaaaagcta ttttaaacat tcgcatcaag ggtcttggtt cagaacaccg
    42901 tacggctatc tttcctaaac ttattttcac tctgaaaaga ggccttaatt tagaggaagg
    42961 aactcccaac tacgacatca agcagttggc tcttgagtgt gcaaccaaac ggatgtatcc
    43021 agatgtttta tcttacgata agattgttga cttgacagga tccttcaagg tgcctatggg
    43081 ttgccgttct ttcctccaag gatggaagga tgaaaatggt gtagaagtca attcaggtcg
    43141 catgaatctg ggtgttgtga cggttaatct gcctcgtatt gctcttgagt ctgaaggtga
    43201 tatgaataag ttctgggaaa tcttcaacga gcgaatgaat atcgcagaag atgctcttgt
    43261 ttaccgtgtc gaacgcacta aagaggcgac accagcaaat gctcctattc tttatcagta
    43321 cggtgctttt ggccatcgtc taggtaaaga agaaagtgtt gaccagctct ttaagaatcg
    43381 tcgtgctacc gtttcgctgg gctatatcgg cttgtatgaa gtagcgacag ttttctttgg
    43441 taacagctgg gaaagtaatc cagatgctaa ggaattcacg ctagacatca ttcacgatat
    43501 gaaacgccgt gtagaagagt ggtcagacca atatggctac catttctcta tctactcaac
    43561 accatccgaa agtctgacag accgtttctg tcgactagat atagacaagt ttggctctat
    43621 tcctgatatc acagacaagg aatactacac caactctttc cactacgatg ttcgtaaaaa
    43681 tccaacaccg tttgaaaaat tggactttga gaaagtctat ccagaagcag gtgcgtcagg
    43741 tggtttcatc cattattgtg agtatccagt ccttcagcaa aatccaaagg ccttggaagc
    43801 tgtctgggat tatgcttatg accgtgtagg ctatctaggc accaatactc cgattgaccg
    43861 ttgctacaag tgtgactttg aaggggattt tgaaccaact gagagagggt ttgcttgtcc
    43921 aaactgtggc aatagcgacc ctaaaacagt agatgtggtg aagcgaactt gtggctacct
    43981 aggtaatcct caagcaagac cgatggtcaa cggccgtcac aaggaaatcg ctgcgcgtgt
    44041 caaacatatg aatggttcaa cgattaaaat agctgggcat caagtaacaa attagaaaga
    44101 aatgaaatgg gaaaatatca actagacgat aaggggcgcg cacaagtgac ccgttatcac
    44161 gagaaacact ctaaaggtgg agctggtaag aaagaacgct tgcttagctt cagagaacaa
    44221 tttttaaaca agaacaagaa aaaataaaag tgagagccag ctctcgcttt tctcatagtg
    44281 ggaggtaagg atggaattac gcagaccaag attagcggat aagaaagctg ttttagatat
    44341 gatgacagag tttgaaaaga gccaatcagc ccatgatgga ggattttggg atacagagaa
    44401 ttttgtgtat gaagagtggt tggaaagcaa tcaggaacag gaaatgggga ttaatctgcc
    44461 tgaaggatgg gttcctgcaa ttcagttagt ggctttttct gagaaaggtc aagcagttgg
    44521 atttcttaat ctccggttgc gcctcagtaa ctttttacta gaagaaggtg ggcacattgg
    44581 ctactccatc cgtccatctg aaagaggcaa gggttatgca aaagagactc tccgtcaggg
    44641 cttgcaagtt gctaaggaaa agaacatcaa gaaagctctg gtgacctgta gcgtgaataa
    44701 tcctgctagc agagcagtca ttctagcaaa tggtggaata tttgaggatg ctcacaatgg
    44761 agtcgagcgt tattggatag aggtagcgaa tgaataatcc aaaaccacaa gaatggaaaa
    44821 gcgaggaact tagtcaaggt cgtatcattg actacaaggc ctttaacttt gtggacggcg
    44881 aaggcgtgcg taactctctc tatgtatcag gctgtatgtt tcactgcgag ggatgttata
    44941 atgttgcgac ttggtctttt aatgctggca ttccctatac agcagaatta gaagagcaga
    45001 tcatggcaga tcttgctcag ccctatgttc aaggtttgac tttgttggga ggggagcctt
    45061 ttcttaatac tgggattctc ttgccacttg ttaagcggat tcggaaggaa ttgccagaca
    45121 aggacatctg gtcctggacc ggctacactt gggaagaaat gatgttggaa actccagata
    45181 aactggaatt cttgtcactg attgacattc ttgtcgatgg aagatatgat cgaactaaga
    45241 gaaatcttat gctccagttt cgaggttcat ctaaccaacg aattatcgat gtgcaaaaat
    45301 cgctcaaaag tgggcaagta gtgatttggg acaagctcaa tgacggaaaa gaaagctatg
    45361 aacaggtgaa gagagaatga agaaaaaaga cttagtagac caactagtct cagagatcga
    45421 gacggggaaa gtcaggacac tgggaatata cggtcatgga gcttcaggta aatcaacctt
    45481 tgcacaggaa ttgtaccaag ctttagattc tactacagta aatttgctag aaacagatcc
    45541 ctatatcacc tccggacgct atttggtagt acccaaggac gcgccgaatc aaaaggtgac
    45601 agccagtctg ccagtggcgc atgaactgga gagtttgcag agagatatcc ttgccttgca
    45661 ggcgggtatg gatgtcttga caattgaaga accttggaag gctagtgagg tcttgtctgg
    45721 agccaaacca attttgattg tcgaagggat gtctgttggt tttctaccca aggaactctt
    45781 tgaaaaaacc atctgtttct acacggatga ggagaccgaa ttaaagcgac gccttgctag
    45841 agatacgact gtgagaaatc gcgatgcatc ctttatattg gctagccatc agatgagacg
    45901 ggagcagtat ctgcgatact ataaagaaac tgagtctaag gcggatatct tagtggacca
    45961 atcagaagat aaatttgatg tcaagaggac ttaacttatg tatagaagaa atatagaaga
    46021 aaatccctaa ttttagaaag aaaaagtagg aaaacagttt catcccaaaa aaacgaaaaa
    46081 acctaacaaa tcccttgcaa tcgcagggga tttgtgttat tctattatgg tgctgtaaat
    46141 tacagcctta gttttgatgc aagaggttgc gacacgctcg gttgcattgc cacgcaacac
    46201 cgcgtcggtt ttcttgtgga gctagcctat tatcttaaat agacgaaaag gagaaaaaga
    46261 tggcaaacaa aaaaatccgt atccgtttga aagcttacga acaccgtacg cttgacacag
    46321 cggctgcaaa aatcgtagaa tcagctactc gtacaggtgc acaagttgcg ggtccaatcc
    46381 cacttccaac tgaacgtagc ctctacacaa tcattcgtgc gactcacaaa tacaaagact
    46441 ctcgcgaaca atttgaaatg cgtacacaca aacgtttgat cgatatcgtt aacccaactc
    46501 aaaaaacagt tgatgccttg atgaaattgg atcttccaag tggtgtaaac gtagaaatca
    46561 aactttaatc taaaatataa aagagcagag gctggtgttt caatctaatt gaacacgggc
    46621 tagactcggt gtgaaaaaga taaacttcct attgtccgct agacactgcg ttagtttcct
    46681 attttcactt tgagtttgac gccctttgta tcttagactt gagcataaaa aacgctcgtt
    46741 aaaaactttt tgaataaaaa atatagaaaa ggaactattt tctcatgaca aaaggaatct
    46801 tagggaaaaa agtgggaatg actcaaatct tcactgaagc tggcgaattg atccctgtaa
    46861 cagttattga agcaactcca aacgttgttc ttcaagttaa aactgttgaa acagacggat
    46921 acaacgctat ccaagttggt ttcgatgaca aacgcgaagt attgagcaac aaacctgcta
    46981 aaggacatgt agcgaaagct aacacggctc ctaagcgctt cattcgtgaa ttcaaaaacg
    47041 ttgaaggctt ggaagttggt gctgaaatta cagttgaaac attcgcagct ggagatgttg
    47101 ttgatgtaac tggtacttct aaaggtaaag gtttccaagg tgttatcaag cgccacggac
    47161 aatcacgtgg accgatggct cacggttctc gttaccaccg tcgtccaggt tctatgggac
    47221 ctgttgcacc taaccgcgta ttcaaaggta aaaaccttgc aggacgtatg ggtggtgacc
    47281 gcgtaacaat tcaaaatctt gaagttgtac aagttgttcc agaaaagaac gttatcctta
    47341 tcaaaggtaa cgtaccaggt gctaagaaat ctcttatcac tatcaaatca gcagttaaag
    47401 ctggtaaata ataaagaaag gggaaatcag tcacaatggc aaacgtaaca ttatttgacc
    47461 aaactggtaa agaagctggc caagttgttc ttaacgatgc agtatttggt atcgaaccaa
    47521 atgaatcagt tgtgtttgat gtaatcatca gccaacgcgc aagccttcgt caaggaacac
    47581 acgctgttaa aaaccgctct gcagtatcag gtggtggacg caaaccgtgg cgtcaaaaag
    47641 gaactggacg tgctcgtcaa ggttctatcc gctcaccaca atggcgtggt ggtggtgttg
    47701 tcttcggacc aactccacgt tcatacggct acaaacttcc acaaaaagtt cgtcgcctag
    47761 ctcttaaatc agtttactct gaaaaagttg ctgaaaacaa attcgtagct gtagacgctc
    47821 tttcatttac agctccaaaa actgctgaat ttgcaaaagt tcttgcagca ttgagcatcg
    47881 attctaaagt tcttgttatc cttgaagaag gaaatgaatt cgcagctctt tcagctcgta
    47941 accttccaaa cgtgaaagtt gcaactgcta caactgcaag tgttcttgac atcgcaaata
    48001 gcgacaaact tcttgtcaca caagcagcta tctctaaaat cgaggaggtt cttgcataat
    48061 gaatttgtat gatgttatca aaaaacctgt catcactgaa agctcaatgg ctcaacttga
    48121 agcagggaaa tatgtatttg aagttgacac tcgtgcacac aaacttttga tcaagcaagc
    48181 tgttgaagct gctttcgaag gtgttaaagt tgccaatgtt aacacaatca acgtaaaacc
    48241 aaaagctaaa cgtgttggac gttacactgg ttttactaac aaaactaaaa aagctatcat
    48301 cacacttaca gctgattcta aagcaatcga gttgtttgct gctgaagctg aataatctaa
    48361 ggaggaaata tcgtgggaat tcgtgtttat aaaccaacaa caaacggtcg ccgtaatatg
    48421 acttctttgg atttcgctga aatcacaaca agcactcctg aaaaatcatt gcttgttgca
    48481 ttgaagagca aggctggtcg taacaacaac ggtcgtatca cagttcgtca ccaaggtggt
    48541 ggacacaaac gtttctaccg tttggttgac ttcaaacgta ataaagacaa cgttgaagca
    48601 gttgttaaaa caatcgagta cgatccaaac cgttctgcaa acatcgctct tgtacactac
    48661 actgacggtg tgaaagcata catcatcgct ccaaaaggtc ttgaagtagg tcaacgtatc
    48721 gtttcaggtc cagaagcaga tatcaaagtc ggaaacgctc ttccacttgc taacatccca
    48781 gttggtactt tgattcacaa catcgagttg aaaccaggtc gtggtggtga attggtacgt
    48841 gctgctggtg catctgctca agtattgggt tctgaaggta aatacgttct tgttcgtctt
    48901 caatcaggtg aagttcgtat gattcttgga acttgccgtg ctacagttgg tgttgtcgga
    48961 aacgaacaac atggacttgt aaaccttggt aaagcaggac gtagccgttg gaaaggtatc
    49021 cgcccaacag ttcgtggttc tgtaatgaac cctaacgatc acccacacgg tggtggtgaa
    49081 ggtaaagcac cagttggtcg taaagcacca tctactccat ggggcaaacc tgctcttggt
    49141 cttaaaactc gtaacaagaa agcgaaatct gacaaactta tcgttcgtcg tcgcaacgag
    49201 aaataatatt aaactagtcg cttaagcaac tagtaaatcc gccagctcgg tagcgctcca
    49261 taggagtgca agccgctgtg gtacaacatt taaaggagaa aatataaaaa tgggacgcag
    49321 tcttaaaaaa ggacctttcg tcgatgagca tttgatgaaa aaagttgaag ctcaagctaa
    49381 cgacgaaaag aaaaaagtta ttaaaacttg gtcacgtcgt tcaacgatct tcccaagttt
    49441 cattggttac actattgcag tttatgacgg acgtaaacac gtacctgttt acatccaaga
    49501 agacatggta ggccacaaac ttggtgaatt tgcaccaact cgtacttaca aaggtcacgc
    49561 tgcagacgac aagaaaacac gtagaaaata aggagaacat aaatggcaga aattacttca
    49621 gctaaagcaa tggctcgtac agtacgtgtt tcacctcgta aatcacgtct tgttcttgat
    49681 aacatccgtg gtaaaagcgt agccgatgca atcgcaatct tgacattcac tccaaacaaa
    49741 gctgctgaaa tcatcttgaa agttttgaac tcagctgtag ctaacgctga aaacaacttt
    49801 ggtttggata aagctaactt ggtagtatct gaagcattcg caaacgaagg accaactatg
    49861 aaacgtttcc gtccacgtgc gaaaggttca gcttcaccaa tcaacaaacg tacagctcac
    49921 atcactgtag ctgttgcaga aaaataagga ggtaaaatcg tgggtcaaaa agtacatcca
    49981 attggtatgc gtgtcggcat catccgtgat tgggatgcca aatggtatgc tgaaaaagaa
    50041 tacgcggatt accttcatga agatcttgca atccgtaaat tcgttcaaaa agaacttgct
    50101 gacgcagcag tttcaactat tgaaatcgaa cgcgcagtaa acaaagttaa cgtttcactt
    50161 cacactgcta aaccaggtat ggttatcggt aaaggtggtg ctaacgttga tgcactccgt
    50221 gcaaaactta acaaattgac tggaaaacaa gtacacatca acatcatcga aatcaaacaa
    50281 cctgatttgg atgctcacct tgtaggtgaa ggaattgctc gtcaattgga gcaacgtgtt
    50341 gctttccgtc gtgcacaaaa acaagcaatc caacgtgcaa tgcgtgctgg agctaaagga
    50401 atcaaaactc aagtatcagg tcgtttgaac ggtgcagata tcgcccgtgc tgaaggatac
    50461 tctgaaggaa ctgttccgct tcacacactt cgtgcagata tcgattacgc ttgggaagaa
    50521 gcagatacta catacggtaa acttggtgtt aaagtatgga tctaccgtgg tgaagttctt
    50581 ccagctcgta aaaacactaa aggaggtaaa taaccaatgt tagtacctaa acgtgttaaa
    50641 caccgtcgtg agttccgtgg aaaaatgcgc ggtgaagcaa aaggtggaaa agaagtagca
    50701 ttcggtgaat acggtcttca agctacaact agccactgga tcactaaccg ccaaatcgaa
    50761 gctgctcgta tcgccatgac tcgttacatg aaacgtggtg gtaaagtttg gattaaaatc
    50821 ttcccacaca aatcatacac tgctaaagct atcggtgtgc gtatgggatc tggtaaaggg
    50881 gcacctgaag gttgggtagc accagttaaa cgtggtaaag tgatgttcga aatcgctggt
    50941 gtatctgaag agattgcacg tgaagcgctt cgacttgcta gccacaaatt gccagttaaa
    51001 tgtaaattcg taaaacgtga agcagaataa ggagaaggca tgaaacttaa tgaagtaaaa
    51061 gaatttgtta aagaacttcg tggtctttct caagaagaac tcgcgaagcg cgaaaacgaa
    51121 ttgaaaaaag aattgtttga acttcgtttc caagctgcta ctggtcaatt ggaacaaaca
    51181 gctcgcttga aagaagttaa aaaacaaatc gctcgtatca aaacagttca atctgaagcg
    51241 aaataataga ctagggaagg agaaatttca atggaacgca ataatcgtaa agttcttgtt
    51301 ggacgtgttg tatctgacaa aatggacaag acaatcacag ttgtagttga aacaaaacgt
    51361 aaccacccag tctatggtaa acgtattaac tactctaaaa aatacaaagc tcatgatgaa
    51421 aacaatgttg ccaaagaagg cgatatcgta cgtatcatgg aaactcgccc gctttcagct
    51481 acaaaacgtt tccgtcttgt agaagttgtt gaagaagcgg tcatcatcta atcaaacctg
    51541 aaaggagaaa actgaaatga ttcaaacaga aactcgtttg aaagtcgcag acaacagcgg
    51601 tgctcgcgaa atcttgacta tcaaagttct tggtggttca ggacgtaaat ttgcaaacat
    51661 cggtgatgtt atcgtggcat ctgtaaaaca agctactcct ggtggtgcgg ttaaaaaagg
    51721 tgacgttgtt aaagcagtta tcgttcgtac taaatcaggt gctcgtcgtg ctgatggttc
    51781 atacatcaaa tttgacgaaa acgcagcagt tatcatccgt gaagacaaaa ctcctcgcgg
    51841 aacacgtatc tttggcccag ttgcacgtga attgcgtgaa ggtggcttca tgaagatcgt
    51901 gtcacttgct ccagaagtac tttaattttt aggaacaaac tagtccccta gcttcaagct
    51961 agggtgccct tatgggcgta agaaaaatca aggagaaacc taatgtttgt aaaaaaaggc
    52021 gacaaagttc gcgtaatcgc tggtaaagat aagggaacag aagctgttgt ccttactgcc
    52081 cttccaaaag taaacaaagt tatcgttgaa ggtgttaaca ttgttaagaa acaccaacgt
    52141 ccaactaacg agcttcctca aggtggtatc atcgagaaag aagcagctat ccacgtatca
    52201 aacgttcaag ttttggacaa aaatggtgta gctggtcgtg ttggatacaa atttgtagac
    52261 ggtaaaaaag ttcgctacaa caaaaaatca ggcgaagtgc ttgattaatc acgaaggaaa
    52321 ggagaagtat aatggcaaat cgtttaaaag aaaaatatct taatgaagta gttcctgctt
    52381 tgacagaaca attcaactac tcatcagtga tggctgtgcc taaagtagat aagattgttt
    52441 tgaacatggg tgttggtgaa gctgtatcaa acgctaaaag ccttgaaaaa gctgctgaag
    52501 aattggcact tatctcaggt caaaaaccac ttatcactaa agctaaaaaa tcaatcgccg
    52561 gcttccgtct tcgtgaaggt gttgcgatcg gtgcaaaagt tacccttcgt ggtgaacgta
    52621 tgtacgaatt cttggataaa ttggtatcag tttcacttcc acgtgtacgt gactttcacg
    52681 gtgttccaac aaaatcattt gatggacgcg ggaactacac acttggtgtg aaagaacaat
    52741 taatcttccc agaaatcaac ttcgatgacg ttgacaaaac tcgtggtctt gacatcgtta
    52801 tcgtaacaac tgctaacact gacgaagagt cacgtgcatt gcttacaggc cttggaatgc
    52861 cttttgcaaa ataatatagg aggtaaatct aatggctaaa aaatcaatgg tagctagaga
    52921 ggctaaacgc caaaaaattg ttgaccgtta tgctgaaaaa cgtgctgcat taaaggcggc
    52981 aggggactac gaaggtttat ctaaattacc tcgcaacgcc tcaccgactc gtttacataa
    53041 tcgttgtaga gttacggggc gcccacattc agtttaccgc aaatttggtc tgagtcgtat
    53101 cgcttttcgc gaacttgcgc ataaaggtca aattcctggt gtaacaaaag catcttggta
    53161 atttaagata tcaagggtgt caaaactcca agtaaaaata ggaaacttga cgaagaaact
    53221 aaagtttcta ggaaagttta tctttttcac acagagttta gcccgggttc aattgggctt
    53281 gccaatttga acccgagcta cagctttggc aaaaaagacc aatttgcttt ggagcattgc
    53341 ttctgcatta aattgtctat ttttgctcgt gctgttacgc tctttgtatc atgtattaac
    53401 tagcaagtgc aacttgcaaa ctactagtaa gaggagaaaa acaaaatggt tatgactgac
    53461 ccaatcgcag acttcctaac tcgtattcgt aatgctaacc aagctaaaca cgaagtactt
    53521 gaagtacctg catcaaacat caaaaaaggg attgctgaaa tccttaaacg cgaaggtttt
    53581 gtaaaaaacg ttgaaatcat cgaagatgac aaacaaggcg tcatccgtgt atttcttaaa
    53641 tacggaccaa atggtgaaaa agttatcact aacttgaaac gtgtttctaa accaggactt
    53701 cgtgtctaca aaaaacgtga agaccttcca aaagttctta acggacttgg aattgccatc
    53761 ctttcaactt ctgaaggttt gcttactgat aaagaagcac gccaaaagaa tgttggtggt
    53821 gaggttatcg cttacgtttg gtaaaatcaa gatacaaagc tcgtaaagaa caaagcaaaa
    53881 ttaggaagtt ggagaagttt gtttacaaac aagccaactt atctattttg cacagttctt
    53941 agagcgtgtt cagttcagct cttgaactaa ataagtatct gaaccccgtg aaaactggcc
    54001 gttctggcct gacaatttaa caggagaaaa taaacatgtc acgtattggt aataaagtta
    54061 tcgtgttgcc tgctggtgtt gaactcgcta acaatgacaa cgttgtaact gtaaaaggac
    54121 ctaaaggaga acttactcgt gagttctcaa aagatattga aatccgtgtg gaaggtactg
    54181 aaataactct tcaccgtcca aacgattcaa aagaaatgaa aactatccac ggaactactc
    54241 gtgccctttt gaacaacatg gttgttggtg tatcagaagg attcaagaaa gaacttgaaa
    54301 tgcgtggggt tggttaccgt gcacagcttc aaggatctaa acttgttttg gctgttggta
    54361 aatctcatcc agacgaagtt gaagctccag aaggaattac ttttgaactt ccaaacccaa
    54421 caacaatcgt tgttagcgga atttcaaaag aagtagttgg tcaaacagct gcttacgtac
    54481 gtagccttcg ttcaccagaa ccatataaag gtaaaggtat ccgttacgtt ggtgaattcg
    54541 ttcgccgtaa agaaggtaaa acaggtaaat aatgttgagt ggttgatcat caaccaccaa
    54601 cctattttcc aactttgtgc atagcacacg atttaaaact aaagaggtga aaactgtgat
    54661 ttcaaaacca gataaaaaca aactccgcca aaaacgccac cgtcgcgttc gcggaaaact
    54721 ctctggaact gctgatcgcc cacgtttgaa cgtattccgt tctaatacag gcatctacgc
    54781 tcaagtgatt gatgacgtag cgggtgtaac gctcgcaagt gcttcaactc ttgataaaga
    54841 agtttcaaaa ggaactaaaa ctgaacaagc cgttgctgtc ggtaaactcg ttgcagaacg
    54901 tgcaaacgct aaaggtattt cagaagtggt gttcgaccgc ggtggatatc tatatcacgg
    54961 acgtgtgaaa gctttggctg atgcagctcg tgaaaacgga ttgaaattct aataggagga
    55021 cactagaaaa tggcatttaa agacaatgca gttgaattag aagaacgcgt agttgctgtc
    55081 aaccgtgtta caaaagttgt taaaggtgga cgtcgtcttc gtttcgcagc tcttgttgtt
    55141 gttggtgacc acaatggtcg cgtaggattt ggtactggta aagctcaaga agttccagaa
    55201 gcaatccgta aagcagtaga tgatgctaag aaaaacttga tcgaagttcc tatggttgga
    55261 acaacaatcc cacacgaagt tctttcagaa ttcggtggag ctaaagtatt gttgaaacct
    55321 gctgtagaag gttctggagt tgccgctggt ggtgcagttc gtgccgttgt ggaattggca
    55381 ggtgtggcag atattacatc taaatcactt ggttctaaca ctccaatcaa cattgttcgt
    55441 gcaactgttg aaggtttgaa acaattgaaa cgcgctgaag aaattgctgc ccttcgtggt
    55501 atttcagttt ctgatttggc ataagaaagg ggataaaatg gctcaaatta aaattacttt
    55561 gactaagtct ccaatcggac gcattccatc acaacgtaaa actgttgtag cacttggact
    55621 tggcaaattg aacagctctg ttattaaaga agataacgct gctatccgtg gtatgattac
    55681 agcagtatct cacttagtaa cagttgaaga agtaaactaa tgaagtttta ggggatgtgc
    55741 actgtaccat cccctaaaac tagatatagt catctatgat gacatcgtat aggcgagttg
    55801 atgggggaga caaccttttc tcccttatcg gcgctagcat tttacaaaag aggagaaaat
    55861 aaaaatgaaa cttcatgaat tgaaacctgc agaaggttct cgtaaagtac gtaaccgcgt
    55921 tggtcgtggt acttcatcag gtaacggtaa aacatctggt cgtggtcaaa aaggtcaaaa
    55981 agctcgtagc ggtggcggag ttcgccttgg ttttgaaggt ggacaaactc cattgttccg
    56041 tcgtcttcca aaacgtggat tcactaacat caacgctaaa gaatacgcaa ttgtgaacct
    56101 tgaccaattg aacgtctttg aagatggtgc tgaagtaact ccagttgttc ttatcgaagc
    56161 aggaattgtt aaagctgaaa agtcaggtat taaaattctt ggtaacggtg agttgactaa
    56221 gaaattgact gtgaaagcag ctaaattctc taaatcagct gaagaagcta tcactgctaa
    56281 aggtggttca gtagaagtca tctaagagag gtgacctatg ttttttaaat tattaagaga
    56341 agctcttaaa gtcaagcagg ttcgatcaaa aattttattt acaattttta tcgttttggt
    56401 ctttcgtatc ggaactagca ttacagttcc tggtgtgaat gccaatagct tgaatgcttt
    56461 aagtggatta tccttcttaa acatgttgag cttggtgtcg gggaatgccc taaaaaactt
    56521 ttcgattttt gccctaggag ttagtcccta tatcaccgct tctattgttg tccaactctt
    56581 gcaaatggat attttaccca agtttgtaga gtggggtaaa caaggggaag taggtcgaag
    56641 aaaattgaat caagctactc gttatattgc tctagttctc gcttttgtgc aatctatcgg
    56701 gattacagct ggttttaata ccttggctgg agctcaattg attaaaactg ctttaactcc
    56761 acaagttttt ctgacgattg gtatcatctt aacagctggt agtatgattg tcacttggtt
    56821 gggtgagcaa attacagata agggatacgg aaacggtgtt tccatgatta tctttgccgg
    56881 gattgtttcc tcaattccag agatgattca gggcatctat gtggactact ttgtgaacgt
    56941 cccaagtagc cgtatcactt catctatcat tttcgtaatc attttgatta ttactgtatt
    57001 gttgattatt tactttacaa cttatgttca acaagcagaa tacaaaattc caatccaata
    57061 tactaaggtt gcacaaggtg ctccatctag ctcttacctt ccgttaaagg taaaccctgc
    57121 tggagttatc cctgttatct ttgccagttc gattactgca gcgcctgcgg ctattcttca
    57181 gtttttgagt gccacaggtc atgattgggc ttgggtaagg gtagcacaag agatgttggc
    57241 aactacttct ccaactggta ttgccatgta tgctttgttg attattctct ttacattctt
    57301 ctatacgttt gtacagatta atcctgaaaa agcagcagag agcctacaaa agagtggtgc
    57361 ctatatccat ggagttcgtc ctggtaaagg tacagaagaa tatatgtcta aacttcttcg
    57421 tcgtcttgca actgttggtt ccctcttcct tggtgtgatt tccattttac cgattgcagc
    57481 taaagatgta tttggtcttt ctgatgttgt tgcctttggt ggaacaagtc tcttgatcat
    57541 tatctctaca ggtatcgaag gaatcaagca attggaaggt tacctattga aacgtaagta
    57601 tgttggtttc atggacagaa cagaataaaa gtatttactg aatcagtaaa tactgaggga
    57661 gtggaggttt aaactctgac atttgtaaga gttggatctc ccctcttcta ttttgttttt
    57721 aaatcggggt gaaaagactt tttgcttcta tttaaaaata aaataaggag atcaaatcat
    57781 gaatcttttg attatgggct tacctggtgc aggtaaggga actcaagcag caaaaatcgt
    57841 agaacaattc catgttgcac atatctcaac aggtgatatg ttccgcgctg caatggcaaa
    57901 tcaaactgaa atgggtgttc ttgctaagtc atatattgac aagggtgaat tggttcctga
    57961 cgaagttaca aatggaatcg taaaagaacg cctttcacaa gatgatatta aagaaacagg
    58021 attcttattg gatggttacc cacgtacaat tgaacaagct catgccttgg acaaaacatt
    58081 ggctgaactt ggcattgaac tagaaggtat tatcaatatt gaagtgaacc ctgacagcct
    58141 cttggaacgt ttgagtggcc gtatcatcca ccgcgtaact ggagaaactt tccacaaggt
    58201 ctttaaccca ccagttgact ataaagaaga agattactac caacgtgaag atgataagcc
    58261 tgagacagta aaacgtcgtt tggatgttaa tattgctcaa ggagaaccaa tcattgctca
    58321 ctaccgtgcc aaaggtttgg ttcatgacat cgaaggtaat caagatatca atgatgtctt
    58381 ctcagatatt gaaaaagtat tgacaaattt gaaataaagc gtttttcaca cttgcaaaaa
    58441 tccgctacaa atgttatact gaaatagtct gacttataat tgttgtctct gtgtctagag
    58501 gcatcgaatc gaaatttatg gaggtgcttt tgcgtggcaa aagacgatgt gattgaagtt
    58561 gaaggcaaag tagttgatac aatgccgaat gcaatgttta cggttgaact tgaaaatgga
    58621 catcagattt tagcaacagt ttctggtaaa attcgtaaaa actatattcg tattttagcg
    58681 ggagatcgtg ttactgtcga aatgagtcca tatgacttga cacgtggacg tatcacttac
    58741 cgctttaaat aatcgaaaaa cttggaggga taagaaatga aagtaagacc atcggtcaaa
    58801 ccaatttgcg aatactgtaa agttattcgt cgtaatggtc gtgttatggt aatttgccca
    58861 gcaaatccaa aacacaaaca acgtcaagga taagatagaa aggagaaaac atggctcgta
    58921 ttgctggagt tgatattcca aatgacaaac gcgtagtaat ctcattgact tatgtttatg
    58981 gtatcggact tgcaacatct aagaaaattt tggctgctgc tggaatctca gaagatgttc
    59041 gtgtacgtga tcttacatca gatcaagaag atgctatccg tcgtgaagtg gatgcaatca
    59101 aagttgaagg tgaccttcgt cgtgaagtaa acttgaacat caaacgtttg atggaaatcg
    59161 gttcataccg tggtatccgt caccgtcgtg gacttcctgt ccgtggacaa aatactaaaa
    59221 acaacgctcg cactcgtaaa ggtaaagctg ttgcgattgc tggtaagaaa aaataatata
    59281 ggaggtaaaa gtcttggcta aaccaacacg taaacgtcgt gtgaaaaaga atatcgaatc
    59341 tggtattgct catattcacg ctacatttaa taacactatt gttatgatta ctgatgtgca
    59401 tggtaatgca attgcttggt catcagctgg tgctcttggt ttcaaaggtt ctcgtaaatc
    59461 tacaccattc gctgctcaaa tggcttctga agctgctgct aaatctgcac aagaacacgg
    59521 tcttaaatca gttgaagtta ctgtaaaagg tccaggttct ggtcgtgagt cagctattcg
    59581 tgcgcttgct gccgctggtc ttgaagtaac agcaattcgt gatgtgactc cagtgccaca
    59641 caatggtgct cgtcctccaa aacgtcgccg tgtataatca tcgcattaca ctgcttttcg
    59701 tttaagaggg agtaactaaa tgatcgagtt tgaaaaacca aatataacaa aaattgatga
    59761 aaataaagat tatggcaagt ttgtaatcga accacttgaa cgtggctacg gtacaactct
    59821 tggtaactct cttcgtcgtg tacttctagc ttctctacca ggagcagctg tgacatctat
    59881 caacattgat ggtgtgttac atgagtttga cacagttcca ggtgttcgtg aagacgttat
    59941 gcaaatcatt ctgaacatta aaggaattgc agtgaaatcg tacgttgaag acgaaaaaat
    60001 catcgaactg gatgttgaag gtcctgctga agtaacagct ggtgacattt tgacagatag
    60061 cgatattgaa attgtaaatc cagatcatta tctctttaca attggtgaag gttcttctct
    60121 aaaagcgact atgactgtta acagtggtcg tggatatgta cctgctgatg aaaataaaaa
    60181 ggataatgca ccagttggaa cacttgctgt agattctatt tatacaccag ttacaaaagt
    60241 caactatcaa gtggaacctg ctcgtgtagg tagcaatgat ggtttcgaca aattaaccct
    60301 tgaaatcttg acaaatggaa caattattcc agaagatgct ttagggcttt cagcacgtat
    60361 tttgacagaa catcttgatt tgtttacaaa tcttactgag attgctaagt caactgaagt
    60421 gatgaaagaa gctgatactg aatctgacga ccgtatttta gatcgtacga ttgaggaact
    60481 ggacttgtct gtgcgttcat acaactgttt aaaacgtgcc ggtatcaata ctgtgcatga
    60541 tttgacagaa aaatctgaag cagagatgat gaaagtacga aatcttggac gcaagagttt
    60601 ggaagaagtg aaactcaaac tcattgattt gggtcttgga ttaaaagata aataaaggag
    60661 gaatacatgg cttaccgtaa actaggacgc actagctcac aacgtaaagc aatgcttcgc
    60721 gatttgacaa ctgacctttt gatcaacgaa tcaatcgtga caactgaagc tcgtgctaaa
    60781 gaaatccgta aaactgttga aaaaatgatt actctaggta aacgtggtga tttgcatgca
    60841 cgtcgtcaag cagctgcttt cgtacgtaat gaaatcgcat ctgaaaacta tgatgaagca
    60901 actgataagt acacttctac tacagcactt caaaaattgt tctcagaaat cgcacctcgt
    60961 tatgctgaac gtaacggtgg atacactcgt atccttaaaa ctgaaccacg tcgtggtgat
    61021 gcagcgccaa tggcgatcat cgaattagta taaaatcatc aattttgttg agtgttatga
    61081 tgatggagtc ttgtgctctt agtctagctc tggtctaccg ctaggatttc ggtcctagcg
    61141 ggaacactca tcataagttg ggatagtaga cgcttgttta cgaaattgtt tttttcttaa
    61201 gaacaacttc gtaagcaggc gtttttgagt attttcgtta gaattatgct atactatttg
    61261 aaaagaatcc tgtttaatgt taaggtttct tattttaaga agaattggag tttacttatg
    61321 aaagccatta taactgttgt tggtaaagat aaatctggaa ttgttgcagg tgtttctggt
    61381 aaaattgcag aattaggatt gaatattgac gatatctctc aaactgtatt ggatgaatat
    61441 tttacgatga tggctgttgt atctagtgat gaaaagcaag attttaccta tcttcgtaat
    61501 gaatttgaag cttttgggca aactttgaat gtaaaaatca atattcagag tgcagcgatt
    61561 ttcgaagcta tgtataatat ctaggaggtc atcatggata ttagacaagt tactgaaacc
    61621 atcgccatga ttgaggagca aaacttcgat attagaacca ttaccatggg gatttctctt
    61681 ttggactgta tcgatccaaa tatcaatcgt gctgcggaga aaatctatca aaaaattacg
    61741 acaaaggcgg ctaatttagt agctgttggt gatgaaattg cggctgagtt gggaattcct
    61801 atcgttaata agcgtgtatc ggtgacacct atttctctga ttggggcagc gacagatgcg
    61861 acggactacg tggttctggc aaaagcgctt gataaggctg cgaaagagat tggtgtggac
    61921 tttattggtg gtttttctgc cttagtacaa aaaggttatc aaaagggaga tgagattctc
    61981 atcaattcca ttcctcgcgc tttggctgag acggataagg tctgctcgtc agtcaatatc
    62041 ggctcaacca agtctggtat taatatgacg gctgtggcag atatgggacg aattatcaag
    62101 gaaacagcaa atctttcaga tatgggagtg gccaagttgg ttgtattcgc taatgctgtt
    62161 gaggacaatc catttatggc gggtgccttt catggtgttg gggaagcaga tgttatcatc
    62221 aatgtcggag tttctggtcc tggtgtggtg aaacgtgctt tggaaaaagt tcgtggacag
    62281 agctttgatg tagtagccga aacagttaag aaaactgcct ttaaaatcac tcgtatcggt
    62341 caattggttg gtcaaatggc cagtgagaga ctgggtgtgg agtttggtat tgtggacttg
    62401 agtttggcac caacccctgc ggttggagac tctgtagcac gtgtccttga ggaaatgggg
    62461 ctagaaacag ttggcacgca tggaacgacg gctgccttgg ccctcttgaa cgaccaagtt
    62521 aaaaagggtg gagtgatggc ctgcaaccaa gtcggtggtt tatctggtgc ctttatccct
    62581 gtttctgagg atgaaggaat gattgctgca gtgcaaaatg gctctcttaa tttagaaaaa
    62641 ctagaagcta tgacggctat ctgttctgtt ggattggata tgattgccat cccagaagat
    62701 acgcctgctg aaaccattgc ggctatgatt gcggatgaag cagcaatcgg tgttatcaac
    62761 atgaaaacaa cagctgttcg tatcattccc aaaggaagag aaggcgatat gattgagttt
    62821 ggtggtctat taggaactgc acctgttatg aaggttaatg gggcttcgtc tgtcgacttc
    62881 atctctcgcg gtggacaaat cccagcacca attcatagtt ttaaaaatta agaaaatagg
    62941 agaaatttta agttctattt aagattagac gt
//
LOCUS       DAVLWA010000003        57370 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLWA010000003 DAVLWA010000000
VERSION     DAVLWA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.003"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(94..843)
                     /locus_tag="VYN80_000167"
     CDS             complement(94..843)
                     /locus_tag="VYN80_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1540148.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            976..3312
                     /locus_tag="VYN80_000168"
     CDS             976..3312
                     /locus_tag="VYN80_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1540149.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3413..4090
                     /locus_tag="VYN80_000169"
     CDS             3413..4090
                     /locus_tag="VYN80_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1540150.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            4077..4874
                     /locus_tag="VYN80_000170"
     CDS             4077..4874
                     /locus_tag="VYN80_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1540151.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4891..5994
                     /locus_tag="VYN80_000171"
     CDS             4891..5994
                     /locus_tag="VYN80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1540152.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQAPANELFPGLQGFDNLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            5995..6075
                     /locus_tag="VYN80_000172"
                     /pseudo
     CDS             5995..6075
                     /locus_tag="VYN80_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            6295..7314
                     /gene="galE"
                     /locus_tag="VYN80_000173"
     CDS             6295..7314
                     /gene="galE"
                     /locus_tag="VYN80_000173"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1540153.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7316..8281
                     /locus_tag="VYN80_000174"
     CDS             7316..8281
                     /locus_tag="VYN80_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1540154.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFSAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(8332..8538)
                     /locus_tag="VYN80_000175"
     CDS             complement(8332..8538)
                     /locus_tag="VYN80_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1540155.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8590..9066
                     /locus_tag="VYN80_000176"
     CDS             8590..9066
                     /locus_tag="VYN80_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1540156.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9075..9746
                     /locus_tag="VYN80_000177"
     CDS             9075..9746
                     /locus_tag="VYN80_000177"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1540157.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIVKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9876..10214
                     /locus_tag="VYN80_000178"
     CDS             9876..10214
                     /locus_tag="VYN80_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1540158.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10217..10846
                     /locus_tag="VYN80_000179"
     CDS             10217..10846
                     /locus_tag="VYN80_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1540159.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGKNWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11086..12237
                     /locus_tag="VYN80_000180"
     CDS             11086..12237
                     /locus_tag="VYN80_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1540160.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12550..13299
                     /gene="truA"
                     /locus_tag="VYN80_000181"
     CDS             12550..13299
                     /gene="truA"
                     /locus_tag="VYN80_000181"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1540161.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13289..14062
                     /locus_tag="VYN80_000182"
     CDS             13289..14062
                     /locus_tag="VYN80_000182"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1540162.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14040..14501
                     /locus_tag="VYN80_000183"
     CDS             14040..14501
                     /locus_tag="VYN80_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1540163.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            14728..16069
                     /locus_tag="VYN80_000184"
                     /pseudo
     CDS             14728..16069
                     /locus_tag="VYN80_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(16197..16400)
                     /locus_tag="VYN80_000185"
     CDS             complement(16197..16400)
                     /locus_tag="VYN80_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540164.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(16393..17475)
                     /locus_tag="VYN80_000186"
     CDS             complement(16393..17475)
                     /locus_tag="VYN80_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1540165.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(17601..18383)
                     /locus_tag="VYN80_000187"
     CDS             complement(17601..18383)
                     /locus_tag="VYN80_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1540166.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(18383..19726)
                     /locus_tag="VYN80_000188"
     CDS             complement(18383..19726)
                     /locus_tag="VYN80_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1540167.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(19825..21141)
                     /locus_tag="VYN80_000189"
     CDS             complement(19825..21141)
                     /locus_tag="VYN80_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1540168.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            21334..22560
                     /locus_tag="VYN80_000190"
     CDS             21334..22560
                     /locus_tag="VYN80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1540169.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            22759..24333
                     /locus_tag="VYN80_000191"
     CDS             22759..24333
                     /locus_tag="VYN80_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1540170.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            24598..25386
                     /gene="codY"
                     /locus_tag="VYN80_000192"
     CDS             24598..25386
                     /gene="codY"
                     /locus_tag="VYN80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1540171.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            25386..25961
                     /locus_tag="VYN80_000193"
     CDS             25386..25961
                     /locus_tag="VYN80_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1540172.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            26043..26128
                     /locus_tag="VYN80_000194"
     tRNA            26043..26128
                     /locus_tag="VYN80_000194"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26077..26079,aa:Leu,seq:aag)
     gene            26411..27541
                     /gene="ugpC"
                     /locus_tag="VYN80_000195"
     CDS             26411..27541
                     /gene="ugpC"
                     /locus_tag="VYN80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1540173.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(27664..27834)
                     /locus_tag="VYN80_000196"
     CDS             complement(27664..27834)
                     /locus_tag="VYN80_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540174.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            28052..28810
                     /locus_tag="VYN80_000197"
     CDS             28052..28810
                     /locus_tag="VYN80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1540175.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            28897..29409
                     /locus_tag="VYN80_000198"
     CDS             28897..29409
                     /locus_tag="VYN80_000198"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1540176.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            29591..30535
                     /locus_tag="VYN80_000199"
     CDS             29591..30535
                     /locus_tag="VYN80_000199"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1540177.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            30544..31221
                     /locus_tag="VYN80_000200"
     CDS             30544..31221
                     /locus_tag="VYN80_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1540178.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            31319..32077
                     /locus_tag="VYN80_000201"
     CDS             31319..32077
                     /locus_tag="VYN80_000201"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1540179.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            32115..33620
                     /gene="lytC"
                     /locus_tag="VYN80_000202"
     CDS             32115..33620
                     /gene="lytC"
                     /locus_tag="VYN80_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1540180.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            34116..34634
                     /locus_tag="VYN80_000203"
     CDS             34116..34634
                     /locus_tag="VYN80_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1540181.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            34764..35270
                     /locus_tag="VYN80_000204"
     CDS             34764..35270
                     /locus_tag="VYN80_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1540182.1"
                     /translation="MTKKIVAIWAQDKEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPGELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            35270..35440
                     /locus_tag="VYN80_000205"
     CDS             35270..35440
                     /locus_tag="VYN80_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540183.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            35472..36704
                     /gene="clpX"
                     /locus_tag="VYN80_000206"
     CDS             35472..36704
                     /gene="clpX"
                     /locus_tag="VYN80_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1540184.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            36713..37300
                     /locus_tag="VYN80_000207"
     CDS             36713..37300
                     /locus_tag="VYN80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1540185.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            37311..37691
                     /locus_tag="VYN80_000208"
     CDS             37311..37691
                     /locus_tag="VYN80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1540186.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            37743..38633
                     /gene="rapZ"
                     /locus_tag="VYN80_000209"
     CDS             37743..38633
                     /gene="rapZ"
                     /locus_tag="VYN80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1540187.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            38630..39607
                     /locus_tag="VYN80_000210"
     CDS             38630..39607
                     /locus_tag="VYN80_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1540188.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            39604..40515
                     /gene="whiA"
                     /locus_tag="VYN80_000211"
     CDS             39604..40515
                     /gene="whiA"
                     /locus_tag="VYN80_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1540189.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(40549..41514)
                     /locus_tag="VYN80_000212"
     CDS             complement(40549..41514)
                     /locus_tag="VYN80_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1540190.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            41650..42507
                     /locus_tag="VYN80_000213"
     CDS             41650..42507
                     /locus_tag="VYN80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1540191.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            42494..43273
                     /locus_tag="VYN80_000214"
     CDS             42494..43273
                     /locus_tag="VYN80_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1540192.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            43297..44649
                     /locus_tag="VYN80_000215"
     CDS             43297..44649
                     /locus_tag="VYN80_000215"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1540193.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            44723..45097
                     /locus_tag="VYN80_000216"
     CDS             44723..45097
                     /locus_tag="VYN80_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1540194.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            45099..45947
                     /locus_tag="VYN80_000217"
     CDS             45099..45947
                     /locus_tag="VYN80_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1540195.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            46213..46980
                     /locus_tag="VYN80_000218"
     CDS             46213..46980
                     /locus_tag="VYN80_000218"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1540196.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            46992..48176
                     /locus_tag="VYN80_000219"
     CDS             46992..48176
                     /locus_tag="VYN80_000219"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1540197.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            48173..50044
                     /locus_tag="VYN80_000220"
     CDS             48173..50044
                     /locus_tag="VYN80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1540198.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(50187..51371)
                     /gene="mntE"
                     /locus_tag="VYN80_000221"
     CDS             complement(50187..51371)
                     /gene="mntE"
                     /locus_tag="VYN80_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1540199.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            51675..54371
                     /locus_tag="VYN80_000222"
     CDS             51675..54371
                     /locus_tag="VYN80_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1540200.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            54384..55175
                     /gene="yghU"
                     /locus_tag="VYN80_000223"
     CDS             54384..55175
                     /gene="yghU"
                     /locus_tag="VYN80_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1540201.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            55291..55701
                     /locus_tag="VYN80_000224"
     CDS             55291..55701
                     /locus_tag="VYN80_000224"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1540202.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            55713..57308
                     /locus_tag="VYN80_000225"
     CDS             55713..57308
                     /locus_tag="VYN80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540203.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        16680 a        10817 c        13155 g        16718 t
ORIGIN      
        1 tctaaaaatg ttttagaagt agaggtgtac tattctagtt ccaatctact atataaatga
       61 aaaccttagc tgtttggcca aggttcattt cttctatgca agttcttctc ttttcttatc
      121 tgggttccag atgaagcttg cgataaagct aaagatgatg gttaggatag cgaggataat
      181 agcaaggatg agggcaggga tgcggataaa ccaccagagt ggatttggtt ttttatcatt
      241 gtgccattgt ttcgtttctt cgtccagacg ttggaattct gtttgaatac gattggcgac
      301 tgtttcaatg ccttcatcat tcattttctt gatatctgag atatcgattg gatttccaaa
      361 gttcatatcg acacgttcac ggctaatcaa gcccttcaaa gtcatgggac cggtgtaggt
      421 aaccggcatg atacggacct tggccatttt ggcaatcagt gctgcgcccc ccttgacatc
      481 gtttgagtgg cggctaccac ttggaaacat gatgagagag cggtcacttt ttttgagaac
      541 gttgatagga tatttgatgg ctgaggcgct gggattttca cggtcgatgg gaaaggcgcc
      601 acacatacga atccaccaac caaagatacg gttggtaaag agttcttttt ttgccataaa
      661 gatgaactgt tttggcttgg tcgcaaaggc catataaaca ggatcccacc aggtacggtg
      721 aggcgcaact aaaatataat tttcatcttg attaggaatt ttatcagtat tatgatagtg
      781 agcattgcca ttgatggacc ataagagcaa tacaactaat ccacgcaaat aagtataaaa
      841 catgcgatct ccttcgattg ttttcttgtt attattatac cttatcaaag gagggctggc
      901 aaacttttcc cttgactaga tacatattta ggatgaaatt agaattctgt taaaaaaaat
      961 gatataatag aatttatgga taaaaataag attatgggat taacccaaag agaagtcaag
     1021 gaaagacagg ctgagggttt ggtcaatgac tttaccgcat cagccagtac cagcacttgg
     1081 caaatcgtta aacgaaatgt ctttaccctt tttaacgctt tgaacttagc cattgctttg
     1141 gctcttgcct ttgtgcaggc ttggagcaat ctggtcttct ttgctgttat ctgctttaac
     1201 gctttttctg ggattgtgac cgagctacga gccaaacaca tggtggacaa gctcaatctc
     1261 atgaccaagg aaaaggtcaa aaccatccgt gatggtcagg aagttgctct taatcctgaa
     1321 gaattagtgc taggagatgt cattcgtttg tctgcaggag agcagattcc tagtgatgcc
     1381 ttggttttgg aaggctttgc ggaagtcaat gaagccatgt taacgggaga aagtgatttg
     1441 gtgcaaaagg aagttgacgg cttacttttg tcaggaagtt tcctagccag tgggtcagtt
     1501 ttatctcaag ttcaccatgt cggtgcagac aactatgctg ccaaactcat gcttgaggct
     1561 aagaccgtta aacccatcaa ctcccgtatc atgaaatcgc tggacaagtt ggctggtttt
     1621 actgggaaga ttatcattcc ctttggtctg gctctcttgc tggaagcctt gcttttaaaa
     1681 ggcctgcctc tcaagtcatc tgttgtaaac tcgtcgacag ctcttttggg aatgttgcct
     1741 aagggaattg cccttttgac cattacttcg ctcttgactg cagttatcaa gctaggtttg
     1801 aaaaaggtct tggtgcagga gatgtactct gttgagacct tggcgcgcgt ggatatgctc
     1861 tgtctggaca agacgggtac catcacccaa ggaaagatgc aggtggaggc tgttcttccg
     1921 ttgacggaaa cgtatggtga agaggctatt gccagcatct tgactagcta catggcccat
     1981 agtgaggata agaatccaac tgcccaagcc attcgccagc gttttgtggg agatgttgct
     2041 tatcctatga tttccaatct tcccttctcg agcgaccgca agtggggggc tatggagtta
     2101 gaaggcttgg ggacagtttt cttaggggca cctgagatgt tgctggattc tgaagtccca
     2161 gaagccaggg aggccttgga gagaggatca cgtgtcttgg tcttagctct cagtcaggag
     2221 aaattagacc atcacaaacc acagaaacca tctgatattc aggctctagc cttgctggaa
     2281 atcttggacc ccattcgaga gggagcagca gagacgctgg actatctccg ttctcaggag
     2341 gtaggactca agattatctc tggtgacaat ccagttacgg tgtccagcat tgcccagaag
     2401 gctggttttg cggactatca caactatgta gattgctcaa aaatcaccga tgaggaattg
     2461 atggccatgg cggaggagac agctattttc ggacgtgttt cccctcatca aaagaaactc
     2521 atcatccaaa cgttgaaaaa agcgggtcat acaacggcta tgacagggga cggggttaat
     2581 gatatcttgg cccttcgtga ggcggattgt tctatcgtga tggcggaggg ggatccagca
     2641 acccgtcaga ttgccaatct ggttctctta aactcagact ttaatgatgt tcctgagatt
     2701 ctcttcgagg gtcgtcgcgt ggtcaataac attgcccaca tcgccccgat tttcttgata
     2761 aagaccatct attccttcct gttagcagtc atctgtattg ccagtgcttt actaggtcgg
     2821 tcagagtgga ttttgatttt ccccttcatt ccgatccaga ttaccatgat tgaccagttt
     2881 gtggaaggtt tcccaccatt cgttttgact tttgagcgaa atatcaaacc tgtcgagcca
     2941 aacttcctca gaagatccat gcttcgtgcc ctaccaagtg ccctcatggt cgtgtttagc
     3001 gttctttttg tgaaaatctt tggaacgagt caaggttggt ctgagttaga aatctcaact
     3061 ctcctctatt atctcttggg gtcaattggt ttcttatccg tatttagagc ctgcatgcca
     3121 ttcactctat ggcgagtcct cttgattgtc tggtcagtag gaggcttcct agccacagct
     3181 ctcttcccaa gaatccaaaa actgcttgaa atttcaacct tgacagaaca aacgttgcct
     3241 gtttatggtg tcatgatgtt ggtctttacc gtgattttca tcctgactag tcgctatcaa
     3301 gctagaaaat aaagaaagac tgcaatctgt ggattgcagt ttttttaggt acaagattgc
     3361 cagccgaaat gtggtataat aagaggtaat agagtttgga aagtgagaga aaatgatttc
     3421 aaagagatta gaattggtag cttcctttgt gtcacagggg gctattttac tagatgtggg
     3481 aagtgaccat gcttatctgc ctatcgagtt ggttgagaga ggccaaatca aaagcgctat
     3541 tgcaggtgag gtggtggaag gtccctatca gtctgcggtt aaaaatgttg aggctcacgg
     3601 cctaaaggag aaaatccaag tccgtttagc caatggcttg gcagcttttg aagagactga
     3661 ccaagtgtct gtcattacca ttgctggcat gggtggtcgt ttgattgcta ggattttaga
     3721 agaaggtttg gggaagttag ctaatgtaga gcgtttgatc ctccagccca ataatcgtga
     3781 agacgacttg cgtatctggc tacaggatca tggattccag attgtagcag aaagcatctt
     3841 agaagaagct ggaaagtttt atgagatttt ggtggtggaa gcaggacaaa tgaagctatc
     3901 agccagtgat gttcgctttg gtcccttctt gtccaaagaa gtcagtccag tatttgtcca
     3961 aaaatggcaa aaagaagctg agaagctaga gttcgccctc ggacaaatcc cagaaaaaaa
     4021 tctggaagaa cgtcaagttc tagtagataa gattcaagct atcaaggagg tgccccatgt
     4081 tagcaagtga agtgatccaa gcttatgaag ccttttgccc tcaggaattt tctatggagg
     4141 gagacagccg tggtctgcaa attggcactt tagacaaggg tatccaaagg gtcatggttg
     4201 ccctcgatat tcgtgaagag acagtggcag aggccattga aaagggtgtg gacttgatta
     4261 tcgtcaagca cgcgcctatc tttcgtccta tcaaggactt gcttgccagc cgtccgcaaa
     4321 atcagattta catcgacctg attaagcatg atatcgcagt ttatgtcagc cataccaata
     4381 ttgatatcgt tgaaaatggg ctcaatgact ggttttgtca gatgctagga atcgaggaga
     4441 cgacttatct gcaggaaaca ggtccagaac gtggaattgg acgtattggg aatattcagc
     4501 ctcagacatt ttgggaattg gcccaacagg tcaagcaagt ctttgaccta gatagccttc
     4561 gaatggtgca ttatcaagag aatgatttgc agaagcctat ttcaagagta gcaatttgtg
     4621 gtggaagcgg gcagtctttc tataaggatg ctttggcaaa gggggcagat gtctatatca
     4681 ctggcgacat ctactaccat actgctcagg atatgctgtc tgatggcttg ttagcattgg
     4741 atccaggtca ctatatcgaa gtgctttttg tggaaaaaat cgcagcactc cttactcaat
     4801 ggaaagagaa aaaaggctgg gaactagaga ttttacctag tcaagcatcg accaatcctt
     4861 tccgtcatat ctagttagaa agtgaagaca atgaaaaaag ttgccattat tggagcagga
     4921 attgtgggag caacagctgc ctactacctc tcgagagaaa gtgacctaga ggtgaccgtt
     4981 tttgaccatg agcaaggtca agccaccaag gccgcagcag gaattatcag tccttggttt
     5041 tccaaacgcc gtaataaagc ctggtataag atggcgcgct tgggggctga tttttatgtg
     5101 gatttattag ctgatttaga gaaatcagga caagaaatcg acttttacca gcgttcggga
     5161 gtctttctct tgaaaaagga tgaatccaag ttggaagaac tctatcaact ggctctccag
     5221 cgcagagaag aatctccctt gataggccag ttagccattc tggaccaagc cccagctaat
     5281 gaattattcc ctggcttgca gggattcgac aacttgctct atgcttctgg tggagcgaga
     5341 gtagatggtc acctcttagt aactcgtttg ctggaagcca gtcaggtcaa gttggtcaaa
     5401 gaaaaagtga ctctgacact cttatcatca ggctaccaga ttggcgaaga ggtgtttgat
     5461 caggttattt tggcaacggg agcttggttg ggggacatgt tagagccttt aggttatgaa
     5521 gtggatgtcc gtcctcaaaa aggacaacta cgagattatc agcttgccca agacatggaa
     5581 gattaccctg ttgtcatgcc agaaggggag tgggatttga ttccctttgc aggtgggaaa
     5641 ttatccttgg gcgctaccca tgaaaatgac atgggatttg atttgacggt agatgaaccc
     5701 ttgctccaac aaatggagga ggcggccttg cctcattacc cagttttagc tagagtgact
     5761 tcaagggctg agcgtgtggg aatccgtgcc tataccagtg atttctctcc cttctttggg
     5821 caggtgccag aattggcagg tgtctatgca gccagtggac taggttcatc aggcctcaca
     5881 actggtccta tcattggtta ccatctagcc caactgatcc aagacaagga gttgaccttg
     5941 gaccctctaa attacccaat tgaaaactat gtcaaacgag taaaaagcga ataatactca
     6001 atgaaaatca aagaacaaac taggaaacta gccgcgggtt actcaaagca ctgctttgag
     6061 gttgtggata gaactgacga agtcagctca aaacactgtt ttgaggttgt ggatagaact
     6121 gacgaagtca gtaaccatac ccatggcaag gcgacgttga cgtggtttga attggatttt
     6181 cgaagagtat aagcatttta ctgaaatttt atcaggtagt ttaggatggc aaatgacatt
     6241 ccctgtcaaa aatggtaaaa taagaaaaaa tgatctgaga atcgaggaaa aaagatgcaa
     6301 gaaaagattt tggtaactgg tggtgccggt tttattggaa cccacactgt tattgaattg
     6361 atccaagcag gccatcaagt tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt
     6421 ttagaagttg ttgaaagaat cacaggagtt gagattcctt tctatgaggc agatattcgt
     6481 gacacagata ccctcagaga tattttcaag caggaagaac cgactggtgt cattcacttt
     6541 gctggtttga aggctgttgg cgaatcaaca cgtatccctc ttgcctacta tgacaacaat
     6601 atcgctggaa ctgtcagcct tttgaaagcc atggaagaaa acaactgtaa aaacatcatc
     6661 ttcagttctt ctgcgacagt ttacggggat ccgcacacag tgcccatctt ggaagatttc
     6721 ccactttcag tgacaaaccc atacggtcgt accaagctca tgctagagga aattttgacc
     6781 gatatttaca aagcagactc agaatggaat gttgtcttgc ttcgttactt taacccaatc
     6841 ggagcccatg agagtggtga tttgggagaa aatccaaacg gtattccaaa caatctcttg
     6901 ccatatgtga ctcaagtagc cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat
     6961 tacgatacgg aagatggaac aggtgttcgt gactatatcc acgttgtcga tttggctaag
     7021 ggtcacgttg cagctttgaa aaaaatccaa aaaggttcag gactaaacgt ttataacctt
     7081 ggaactggta aaggttactc agttcttgaa attatccaaa acatggaaaa agcggtggga
     7141 cgtcctattc cttaccgcat cgtagaacgt cgcccaggtg atatcgctgc ctgctactca
     7201 gacccagcaa aagctaaagc agaactcggt tgggaagcag aactcgacat cacccaaatg
     7261 tgtgaagacg catggcgttg gcaaagcaag catccaaatg gatttgaaga ctaagatgat
     7321 gatttcaatc atcgtccctt gtttaaacga agaggaagta cttcctcttt tttatcaggc
     7381 tctggaagct ttacttccag atttggaaac agaaatcgag tatgtctttg tcgatgatgg
     7441 atcaagtgat gggaccttgg aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca
     7501 ttatatttct ttctctcgaa attttggcaa agaagcagcc ctttatgcag gcttgcaata
     7561 tgcgacagga gatttggtgg tggtgatgga tgcagacctc caagatcctc ctagtatgtt
     7621 gtttgagatg aaaaatgtac tagacaaaaa tgtagacttg gactgcgttg ggacacggag
     7681 aactagtcgg gagggagaac ccttctttcg cagtttctct gctgttctct tttatcgcct
     7741 catgcaaaaa atcagcccag tagctctgcc gtcgggtgtc cgtgattttc gtatgatgag
     7801 aaggtctgta gtcgatgcca ttttaagctt gaccgagtcc aatcgttttt caaaggggct
     7861 cttcgcctgg gtgggtttca aaacctacta tttggactat ccaaatgtcg aaaggcaggc
     7921 tggcaagaca agttggagtt ttaggcaact ctttttctac tccattgaag ggattattaa
     7981 tttttcagat tttcccttga ttatagcctt tgtagctggt ctcctatctt gttttctttc
     8041 tctgctgatg accttttttg ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg
     8101 ttggacctct ctgatggctg ttattctctt tcttggaggc attcaactct tgaccattgg
     8161 gattctcggc aagtatatca gtaagattta tttagagact aaaaaaagac cactttatct
     8221 tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa aataaagtga aaagactata
     8281 attttacatg gaaatgtgct aaactagagg gagtggatta tcgccattga tttaggcttc
     8341 ctggtttcca atcagggcgc gagttgggca atttttaaca gcctctaaga catcctgact
     8401 aggagaaatt tctttttcca gttggtcagg gtcatcgtaa aaacgcacga ttccattatc
     8461 gtggtaatca aataaatcag aataagtttg gcaaagccca caggcgatac atcgttcagg
     8521 tataagtgtg attttcatgt tcatatttat attgtaataa gaaaagtaaa aaaaaacaag
     8581 gagtaaggta tggcaaaaga accgtggcaa gaagatatct atgatcaaga agaatcaaga
     8641 gcagagcgtc ggcatcgaaa ccacggaggg gcggatagga tggctaatcg tattttgacg
     8701 atcctagcta gtattttctt tgtaattgtg gtggtgatgg tcatcgttct catctatcta
     8761 tcatcggggg ggagtaatcg cacagcagcc ttaaaagact ttcatgattc tgatgcaagt
     8821 gtagtacaaa tctcatcttc aagcagttct cagcctgagc agagttcaga gccagaatct
     8881 acttctagta gttcagaaga agctgctaat cctgaaggaa cgattaaagt tctcgcagga
     8941 gaaggggaag cagctattgc cgctcgtgca ggaatctcca ttgctcagtt agaggccttg
     9001 aatcctgggc acatggctac aggatcttgg tttgctaatc caggtgatgt tataaaaata
     9061 aaataggagc cgatatgaaa acaattcaaa ttgctattga tggtcctgct tccagcggta
     9121 agagtacggt cgcaaagatt attgctaagg attttggatt cacctacctt gatacaggag
     9181 ctatgtatcg tgcagcgacc tatatggctc ttaagaacca attaggagtt gaagaagtcg
     9241 aagcccttct agccttgttg gaccagcatc caatcagctt tggacgttca gaaactggag
     9301 accagcttgt ttttgtagga gatgtggata ttacccatcc tatccgtgaa aatgaagtga
     9361 ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg tgagaaactg gtttctctcc
     9421 aacaagaaat tgcccagcaa ggcgggattg ttatggatgg tcgcgatatt ggaactgttg
     9481 tattgccgca agcagaattg aaaattttcc tagtagcttc tgttgatgag agagcagagc
     9541 gtcgttacaa ggaaaatatt gtcaagggaa ttgaaacaga ccttgaaacc ctaaaaaagg
     9601 aaattgctgc gcgtgactac aaggatagtc atcgtgagac ttctcctctc aaacaagcag
     9661 aggatgctgt ctaccttgat acaactggtt tgaacattca agaagtagtt gaaaaaatca
     9721 aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg atagattgat atcggttttt
     9781 tctaaatttg tcaaaatgct gattttaggg tataatagtt gctgttcgag aaattttgat
     9841 tttcaaagaa tagtgaatga tgataaggag aaatcatgaa caatttacca aattgtccaa
     9901 aatgtaactc agagtatgtc tacgaagacg gtgccctact ggtttgccca gagtgtgctc
     9961 atgagtggaa tcctgctgaa gttgcagaag tagaagaagg tcttgtcgct atcgatgcca
    10021 acggaaataa attgactgat ggtgatacag taactctcat caaggacttg aaagtaaaag
    10081 gtgcgccaaa agatttgaaa caagggacgc gcgtgaaaaa tatccgcatc gtagaaggcg
    10141 atcacaatat cgactgtaag attgatggtt ttggtgccat gaaacttaaa tcagagtttg
    10201 tgaggaagat ttaagcatgt caaaacacta taaacttgta ttttatagcc gtatcttctt
    10261 gtttctagcg gcttttacgg gagtttatct tgaaattact aggcatggtg gttttgggat
    10321 gcttctctat tacacggttc tgtccaacct cttggtaact atttttaccc tttatcttct
    10381 aaaggttatg agccgtgtag gtaaaaattg gcaaaggcca agtctcttgc gcttaaaagg
    10441 tggggtcacc atgagtatca tgattacctg tgtgatttac catttcctct tggcgcccat
    10501 tgcgactaat ttctataccc tagaaaattt cctttgccac tatatcgttc ccatctggtt
    10561 tttagcggat accctctttt ttgacaaaca gggtcaatac aagatttggg atccagcagt
    10621 gtggaccatt ttaccctttc tgtatatgat gtttgctctt tttaatggct tggttctaaa
    10681 actcaatatt ccaaatgcca aggataatcc tttcccttac ttctttttga atgtgaacaa
    10741 gggttggaat gttgtgttta agtggtgtct gattatcttt gttgcctata tggtagcagg
    10801 atttattttc tactttatca agcaaatcaa gagaaagtca tcctaatact cttcgaaaat
    10861 ctcttcaaac cacgttagtt tccatctgca acctcaaaac actgttttga acaacctgcg
    10921 gctagctttc tagtttgctc tttgattttc attgagtatg agatacaagg gattcatttt
    10981 cgaatttaag aaggagtcta caaaccaatg aggcttcttc ttttcttgct tatttgataa
    11041 ctatcaaaaa agtagaagat taagagaaat atagaaagag atttcatgaa acaattttta
    11101 gaacgggcca gcattttggc tctctccctc gttttgatta cctccttttc catttcgagt
    11161 gccctaccag ccatgtttga ctattatcag ggttattcta aggaacaaat tgagctcttg
    11221 gtgagcttgc cttcctttgg aatcatgatg atgttactgc taaatggttt cttagaaaaa
    11281 atatttcctg agcgcttaca gattagtttg ggcttgctga ttttatcatt gagcggtaca
    11341 gctcccttct ggtaccaagc ctatcccttt gtctttggaa cacggcttct ctttggtttg
    11401 ggtcttggga tgatcaatgc caaggccatt tctattatca gtgaacgcta ccaaggaaaa
    11461 aggcgaattc agatgttagg gctacgcgct tctgcagagg tcgttggagc ttctctcatt
    11521 accttggccg tcggtcagtt gttggccttt ggttggacag ctatctttct agcctatagt
    11581 gctggatttt tggtgatgcc cctttatctg ctctttgtcc cttatggaaa atcaaagaaa
    11641 gaagtcaaga aaagagcgaa ggaagcaagt cgtttaactc gagaaatgaa aggcttgatt
    11701 tttaccttag ctatcgaagc ggcagttgta gtttgtacca atacagctat taccatccgt
    11761 attccaagtt tgatggtgga aagaggattg ggggatgccc agttatctag ttttgttctt
    11821 agtatcatgc agttgatcgg gattgtggct ggggtgagtt tttctttctt gatttctatc
    11881 tttaaagaga aactgctcct ctggtctggt attacctttg gcttggggca aatcgtgatt
    11941 gccttgtctt catccttgtg ggtggtagta gcaggaagtg ttctggctgg atttgcctat
    12001 agtgtagtct tgacgacggt ctttcaactt gtctctgaac gaattccagc taaactcctc
    12061 aatcaagcaa cttcatttgc tgtattaggc tgtagtttcg gagcctttac gaccccattt
    12121 gttctaggtg caattggctt actaactcac aatgggatgt tggtctttag tatcttagga
    12181 ggttggttga ttgtaatctc tatctttgtc atgtacctac ttcagaagag agcttaggat
    12241 tgattcctaa gtttttcttt tgatactcaa tgaaaatcaa agagcatagt tgattgagtt
    12301 tggaatagta cgctgtagct gctaaaatat ttctagaaat taatttgact tttctaatag
    12361 agttattcat atcttatttc aatctactat agaaagctag ccgcaggttg ctcaaaatag
    12421 tgttttgagg ttgcagatgg aaactgacgt ggtttgaaga gattttcgaa gagtatgaga
    12481 tttttatacc catacaatat ttgttttagg tccttgttta cagactaatt tcttgataaa
    12541 atagaggtta tgacgagata taaagcaact atttcctatg atggttatgc ctttgctggc
    12601 tttcagcgcc agtctcatgc gcgtagcgtt caggaagaaa ttgaaaaaac cttgaccaga
    12661 ttaaataaag ggcaaaccat tactgttcac ggtgctggta ggacagatag tggggttcat
    12721 gccctgggac aggtcattca ttttgacctg ccttatcaga tggatgagga gaaactccgt
    12781 tttgccttgg atacccagtc tcctgaagat attgatgtga tttcgattga gcttgtggca
    12841 gatgattttc attgccgtta tgccaaacat agcaagacct atgagtttat tgtggataga
    12901 ggacgtccca aaaatcctat gcgccgtcac tatgccaccc actttcccta cccactcgat
    12961 gtggaacgaa tgcagattgc aatcaaaaag ctagagggaa cccatgattt taccggtttt
    13021 acagcctctg ggactagtgt agaggataag gttcgcacca tcacagaagc tagtttaata
    13081 gtcgatgaga caggacaatt tttgaccttt accttttcag gaaatggttt cttgtataaa
    13141 cagattcgca atatggtggg gactcttctc aaaatcggta acaaccgcat gccagtagag
    13201 cagattgatc tcatcttgga gaagaaggac aggcaacttg caggtcccac tgcagcacca
    13261 aatggtttgt atttaaagga gattcgttat gaagaataat cgtattttag cactttctgg
    13321 aaatgatatt tttagtggtg gtggactgtc agctgatttg gctacctata ccttgaacgg
    13381 cttgcatggg tttgtagcag tgacttgttt gacagccttg acagaaaaag gatttgaagt
    13441 ctttccaact gatgatacca tttttcaaca tgaattagat agcttacgtg atgtggaatt
    13501 tgggggaatt aagattggtc ttctccctac tgtcagtgtg gctgagaagg ccttggactt
    13561 tatcaaacaa cgcccaggag tacctgtggt gttggatcct gtcttggtct gcaaggaaac
    13621 gcatgatgta gctgtcagtg agctctgcca agagttgatt cgctttttcc cttatgtcag
    13681 tgtgattacg cctaatctcc cagaagcaga attattatcc ggtcaggaaa ttaaaacctt
    13741 ggaagacatg aaaactgcag cgcagaaatt gcatgattta ggagcgccag cagtcattat
    13801 caagggaggc aatcgtctta gtcaggacaa ggctgtggat gtcttttatg atggacagac
    13861 ctttactatc ctagaaaatc cagttatcca aggccaaaat gctggtgcag gttgtacctt
    13921 tgcctctagc attgccagtc acttgattaa aggtgataaa cttttgccag cagtagaaag
    13981 ctctaaggct ttcgtttatc gtgctattgc acaagcagat cagtatggag taagacaata
    14041 tgaagcaaac aaaaacaact aaaatcgccc ttgtatccct attaaccgcc ctttctgtgg
    14101 ttctaggtta tttcttaaaa atcccaacac ctacaggaat tctaactctt ttagatgctg
    14161 gtgtcttctt tgcggccttt tactttggta gtcgtgaagg agcggtagtc ggaggactag
    14221 caagtttctt gattgacctc ttatcaggct accctcagtg gatgttcttt agcttggtca
    14281 accatggctt gcagggattt ttcgcaggat ttaaaggaaa aagtcagtgg ttaggcctta
    14341 ttttagcaac tattgccatg gtaggaggct acgccttggg ttctactttg atgaatggct
    14401 gggcagcagc cctcccagaa attctaccaa atttcatgca aaatatggta gggatgattg
    14461 taggatttat tcttagtcaa agtatcaaga agattaagta atactcttcg aaaatctctt
    14521 caaaccacgt cagcttccat ctgcaacctc aaaacactgt tttgagcagc ctgaggctag
    14581 ctttctagtt tgcactttga ttttcattga gtataaagag gttgagtaaa aagtatttca
    14641 aataagaaaa acgcatagta tcaggtgttg aatgaactgc cccccaaaag ttagacagaa
    14701 aaaatctaac ttttggggtg ttttattatg aaactaactt atgatgataa agttcagatc
    14761 tatgaactta gaaaacaagg atatagctta gagaagcttt caaataaatt tgggataaac
    14821 aattctaatc ttaggtacat gattaaattg attgatcgtt acggaataga gttcgtcaaa
    14881 aaagggaaaa atcgttacta ttctcctgat ttaaaacaag aaatgattca taaagtctga
    14941 catgaaggct agactaaaga tagagtttct cttgaatacg gtctcccaag tcgtacgata
    15001 cttcttaact ggctagcaca atacaggaaa aacgggtata ctattgttga gaaaacaaga
    15061 gggagagtac ctaaaatggg acgtaagcca aaaaagagac ctgaagagag gacagaatta
    15121 gaacgtcttc aagcagaaaa tgagtacctg agagtggaga atgccatcct aaaaaagtta
    15181 agagaactcc gattgaagga ggaaaaagag aaagacagaa attgtttaag aattaatgac
    15241 tgagttttcg ttagatcttc ttctaaaagc cattaaacta gctcgttcga cctactacta
    15301 tcacttgaaa cagctagaca aaacagataa ggaccaagag cttaaagctg aaattcaatc
    15361 catttttatc gaacacaagg gaaattatgc ttatcgtcga attcatttag aactaagaaa
    15421 tcgtggttat ctggtaaatc ataaaagagt tcaaggcttg atgaaagtac tcaatttaca
    15481 agctaaaatg cgacagaaac gaaaatattc ttctcataaa ggagacgttg gtaagaaagc
    15541 agagaatctc attcaaggcc aatttgaagg ctctaaaaca atggaacagt gctacacaga
    15601 tgtgactgaa tttgccattc cagcaagtac tcaaaagctt tacttatcac cagttttaga
    15661 tggctttaat agcgaaatta tcgcctataa tctttcaact tcacccaact tagaacaagt
    15721 acaaacaatg ttggaacagg cattcacaga gaagcactac gagaatacga ttctccatag
    15781 tgaccaaggc tggcaatatc aacacgattc ttatcatcag tttcttgagg gtaagggaat
    15841 tcaagcatcc atgtcacgca agggaaacag ccaagacaac ggtatgatgg agtccttctt
    15901 tgggattctg aaatcggaaa tgttttacgg ttatgagaag tcgtttcagt cgcttaagca
    15961 attggaacaa gctattgtag actatattga ttactacaac aataaacgaa ttaaggtaaa
    16021 actaaaagga cttagtcctg tgcaatacag aactaaatcc ttcggataaa ttatttgtct
    16081 aactgtttgg gggcagtaca ttttaacact tgatactatg cgttttattg tgggaagatt
    16141 ttcctcattt tctagtgaaa ttgaactttt tctaacatct tttgatagat gacttcttaa
    16201 tcaattatca tttccattgt tgtttcttgg ggtttcatcc cttgtctttg ataaaagcgg
    16261 agagcaccct cgttatcatt ccagacactt agggttagat tattgcagcc aatttcttta
    16321 gcataatagc agaaaaatga caataaaata gcccctagat attttgaaag gaaagcatag
    16381 gggctatata gtttaatcaa aataaagcaa atctttgctg gtgcttgtaa agaggtcgaa
    16441 gccatccttg gtaacaacac cgcagtcttc aatacgaaca ccgactttac cagggatata
    16501 gatacctggt tcaacagaga agcacatgcc ttcttcgatg accatgtcgt ttccttccat
    16561 gatagatggg aattcatgga catccatacc gataccatgc ccgagacggt ggttgaagta
    16621 ctcaccataa ccagcttttt cgatgacctc acgggcagcg cggtccactt catgagcagt
    16681 cacacctggc ttgataaagt caagagcagc ttgttgggct tcaagagtca agttgtaaat
    16741 atctttcttg aattggtctg gtttgccgac agcgactgta cgagtcatat ctgacgcata
    16801 gccattgacc agaacaccca ggtcaaagag gagaagagca tcattttcaa ccttattagc
    16861 tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc aagaccatgg tatcaaagct
    16921 catttcataa ccttcacgtt tcatggcaaa gtcgatttgt gcgatgatat ctgtctcagt
    16981 cttatcaaga gaaatattgt caaaaccaac atgaacagcc ttgtcagcat aaagacctgc
    17041 aaccatcatt ttttgcactt catcagctga tttgatgagg cgcatgcgtt ggatacgagg
    17101 agtgaggttg tcaaactcag cagtctcaaa aactgttttc aaaccatggt atttggtcaa
    17161 gatgagattg tcaaactcaa cagcgacacg tttgaagtca agttgtggaa gagcatgttt
    17221 gattttttgc catggatttt cagaatcgac atagcccact actgggaagg aaacggtgct
    17281 acttgcacgt tctacttcaa gagctgggac aaagaggaga ggttcctgat ccgctaggac
    17341 aaagaggaac atttggcgtt catggggatc actgtaaaaa ccagtgaggt aattgattgt
    17401 gacggggtca gatacgacag cgacgtctag tttttctgat tcaagatatg ttaggatttg
    17461 ttgtaattta gacatatgct accttctttc tacccctcta ttttggcaaa aaatgagaga
    17521 aaatgcaagg gagaagggta aaagaaaaga caaaaaactc cgacaagata acggagttct
    17581 ttgatatcat tttcctttgt ttaagaaaag tcagccttgc ttttgacgtc actgtactct
    17641 tcagcgattt cttggctgag aatgtcctca taggcaggga gttggatgtc ctgaccatat
    17701 ttcttcttga gggcagtagt aactaggcga taagccagat tggcattacg agagaggata
    17761 ggcccgtgga agtaggaacc aaagacattc ttataatgaa ccccttcacc gaccttttct
    17821 tcgttgtttc catttccata gacaacctgt cccagcggtt tttggtcatc agagaggaag
    17881 gtacgacctt ggtgattttc aaatccatag taggtttcat cgaaatcttc attgtgaatc
    17941 ttgatgtcac cgataaaacg gttattggtc tggttgagcg tgtagtgtcc catgacccct
    18001 agcccttcga tacgttttcc tgaagcttca acataatatt gacccaatag ttggaaacca
    18061 ccgcagatag ccagaactac accgtcgttt tggatgtagt tgtcaatgct ctctttttta
    18121 gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt gaccaccacc gaaaaaggcg
    18181 atgtcgtagt gattttcatc aaagtcatca tggagagaaa cgatgtcaac ggtcacatgg
    18241 gctcccagtt tttcagccac atacttgagc atgaggatgt ttccattgtc cccgtaggta
    18301 ttcatgagat ttccgtagag gtgggcaatg ttgagctgat aggggtaatt gctatctttt
    18361 gaggaaagtg aagtataaac cattagttca tctcctttct aacaatctga cgactagcca
    18421 gcagttcacg aaattccagc atggcagtat aagttgccag aatataggca tgcttgcagt
    18481 cttgattctc aatggtcttg agaacttgct ccagattact cgtttcagtg attttctcag
    18541 ctggatagcc agtcactcgg aggcgacgag cgatttcaga atgacgaaca ccgccagcgt
    18601 tgatttcagg aatgtccatg tcagtgattt gctcaaagtc tgcatcccag atccagctag
    18661 tgtcaattcc atctgcatag ttggcattaa ggaggacaga taggctaaat ggataaggtg
    18721 ctagtttgat catttcgata gcttgggttg caccgactgg atttttaatc aagacaaggg
    18781 tacattcctt gtcaccgata tgaaaggttt cttggcgtcc aaagacagca cggctcttgt
    18841 caaatccctg tttgatgagt tgggaatctg cgccgaggaa acgggcgatg gccacagcag
    18901 ctagggcgtt atagatatta tagagcccgc cgatttggat accgtattct tggccgtcta
    18961 tgacaaagcg agagcgattg ttggtcaact caaccagttt tgtcaaacga tagtcgagat
    19021 caggacgttt acatccacaa ccttcacaga tataggcacc caagtttgca taggtattat
    19081 gctcatattt gaggatgcct tggcagtcag gacagagaat cccttcggta ttgtagtgag
    19141 caagttgggc tggtcctttt tccaagtcaa aaccaaaata ctctataggg tttggaatag
    19201 ttggcttgta gaaaagtgga ctgtctccgt taaggagaac agtagcagtt ggaacttttc
    19261 gaatggcatc caatatcatg ttataggtag tatagatttc accgaaacgg tccatctggt
    19321 cacggaagat attagtaatg acaaaaagac taggctggat atagtcacag atacgagata
    19381 gactggcttc gtcaatttcg aggacggcaa tatttttccc agtttttgaa gatttggctg
    19441 ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc gcttgggttg gttagaactt
    19501 gaccataaac ctcttttaaa atgccgacag tgagggcagt tgtcagggtt tttccatttg
    19561 ttccagtgac aacgacaatc tcgtagttct tagctaggct ttgtaaaata tctttatcaa
    19621 attgaagggc gactttccct gggagcgtac ttccacgtcc aagacggctt aaaacgaagt
    19681 gggaagaacg cccagcaaga aggcccaaag tagtttttaa gttcatgttt ctattatatc
    19741 acaaaagagg gaaaagacag atttggtgtt tcgtagaagt aaaaacagcc caaagagggc
    19801 tgtcagttaa gatgagattt caacttaaat cgcaaacaag tcattcaagt tctcagccaa
    19861 ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa ggaatcttgt tgtccatagc
    19921 aacagtaatg atgttgatga tttcttgaga accttctgag aagatgcttg caccaagaat
    19981 ttcttttgtt tcagtattaa caacagcttt gaaagctccg cgaaggtctc catttacgtg
    20041 accacgaggc atggctgcaa cagggatttc tttcactgcg tatggaagtt tcaaatcagc
    20101 tgcttggctt tcagtcaaac caacttgtga aagtgcaggt gtgatgaaca tagtatttgg
    20161 cacattgaga cggtcctcaa gtgtgtagct gccatctcca gcaaggtagc tgtaaacaac
    20221 acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca ccgttgacat ctccaactgc
    20281 aaagacacca ggaacgtttg tttgacagtg tttgtctact ttaatagcac cacgttcagt
    20341 tagttcaata tctgtatttt caagttgaag tggttctaca tttggtttgc gtccagttgc
    20401 gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt acgacaagca cttggtcacc
    20461 atcgtttttg atttcagtag tatggatatt ttgaagcaat tcaatgccat cttcttccat
    20521 gtattgttta gcaagagctg cgatggaagg ttctgcacga ggtaggaatg tatccaaggc
    20581 atctaggact gtgaccttgc ttccaagttt gttgtaaagg ccggcaaatt caagaccgat
    20641 atttccgcca ccaaggattc caagtttttc aggtaatttg tccaagcttt ggatacctgt
    20701 tgagtcaaag atgtttttgc ttgtagcaag tccaggaatt ggcaagacgt ttgaaacagc
    20761 accagtgttg atgacgattg tttcagcagt cagttctttc ttttcatcac cagcttggat
    20821 ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca aagatatcta cgcctgtacc
    20881 agcaacagtc gcatagtttt taccgttgag gcgaccagtg atcgtgtttt tagtagcaat
    20941 cacttcttca aaagacaagt ccttttcagc agcaactagc aaggttttag ttgggataca
    21001 accaatgttg atacaagttc caccgtacat agccttgctg cgttcaacga gggcaacttt
    21061 tttgccagct gaagccaatt tacctgctag tgttttacca gctttaccaa atccaataac
    21121 aattaaatcg tatgttaaca tttatcttcc tcctattcga atttcattct agcacaagaa
    21181 aaaaactttt gcacaattct gctacggaaa agacagaaaa agataaaaac gctttccaga
    21241 caaaaaacta gtaatttttt gacaagcttg ataaactaaa atcctatcta tttttatata
    21301 agaaagaata aatgttcgaa aataggaggt tttatgaaat cgaatcgtta tattattgcc
    21361 tttgctgggg tcattttaca cttaatgctg ggttcgactt atgcttggag tgtttatcgt
    21421 aaccctatta ttgaaaaaac gggatgggat caggcttctg ttgccttcgc ctttagtcta
    21481 gcaatctttt gtttgggctt atcggctgca tttatggggc gtttggtaga aaaatttggt
    21541 ccgaaagtca tggggagtct atctgctttt ctatacgcag gtggaaatat cttaacagga
    21601 tttgcaatag accgtcagga actgtggttg ttgtatctag cttatggcat tttaggtggg
    21661 cttggtttgg gagcaggcta tattacccct gtgtcaacga ttataaaatg gtttcctgat
    21721 aaacgtggtc tcgcaacagg tttagcgatt atggggtttg gttttgcttc tttattgact
    21781 agtcccatag cgcaacacct catcgcaggg gtagggattg tagaaacttt ttatatttta
    21841 ggagcaagtt actttattat catgctccta gcttcacaat tcattaagcg tccaaatgag
    21901 caagagcttg caattttatc ttcttcaggg aaagaaaaaa cagcctcttt gacgcaagga
    21961 atggctgcaa atcaggctct aaaaagcaat cggttttata tgctttggat tattttcttt
    22021 atcaacatag cttgtggttt aggcttaatt tcagcggcat cgccaatggc acaggagatg
    22081 gctggcttgt ctacaagtca tgcagcagta atggtgggtg ttttggggat tttcaatgga
    22141 tttggtcgct tgctctgggc gagtttgtct gactatatcg gtcgccctct aacctttagt
    22201 atattactgc ttgtcaatct tttcttttct ctctcacttt ggctctttac agattccgtt
    22261 ttatttgtag ttgctatgtc tattttgatg acttgctatg gagctggttt ttctttgatt
    22321 ccagcttatc tcagtgatat ttttggaacc aaggaattgg ccgctctgca tggttatatt
    22381 ttaacagctt gggcaatggc tggtttagcg ggacctattt tattagcaga gacttataaa
    22441 atggctcatt cgtacacaca aaccttgttc gtttttctca ttttatacag tatcgccttg
    22501 gctttgtctt attatctagg tcgttcaatc aaaaaagaaa gtcaaaaacc gcttacatga
    22561 ttttctgtag aaatggggct atcttttgct ctggtcttgt gttatactag ataggttgca
    22621 aagaaaacag tacttttctt ttgtggaaaa gaagcaacac gattttatat ctagttatat
    22681 gaaaatacgt cataaaaaga aaagtataaa ctaagcccta tagggtggct tcgcaccacc
    22741 tttagaaaga agaataacgt gaaatttaat gaattaaact tgtctgctga tttgctagca
    22801 gaaattgaaa aagctggttt tgtagaagct agtcctatcc aagaacaaac tattcctttg
    22861 gcccttgaag gcaaggatgt tatcggtcaa gctcagactg gtacaggaaa aactgcagcc
    22921 tttggcttgc ctacccttga aaaaatccgt atagaagaag cgactattca agccttggtc
    22981 atcgctccaa ctcgtgaact agctgtccaa agtcaagaag aactcttccg ctttggtcgt
    23041 agtaagggag tcaaagtccg ttcagtatat ggcggatcaa gcattgaaaa acaaattaag
    23101 gctcttaaat ctggtgccca tatcgtggtg ggaactccag gtcgcctctt ggacttgatt
    23161 aaacgcaagg ccttgaaatt acaagacatt gaaaccctta tccttgacga agcggatgaa
    23221 atgcttaaca tgggcttcct tgaagacatc gaagccatta tttcccgtgt acctgagaac
    23281 cgtcaaactt tgcttttctc agcaactatg ccagatgcca tcaaacgtat cggtgttcag
    23341 tttatgaaag cccctgaaca tgtcaagatt gcggctaagg aattgacaac agaattggtt
    23401 gaccagtact atatccgtgt taaggaacaa gaaaaatttg acaccatgac tcgtctcatg
    23461 gatgtggcac aaccagaact cgctattgta tttggtcgta ccaaacgccg tgtggatgaa
    23521 ttgactcgtg gtttgaaaat tcgtggcttc cgtgcagaag gaattcatgg cgacctagac
    23581 caaaacaaac gtcttcgtgt ccttcgtgac tttaaaaatg gcaatcttga tgttttggtt
    23641 gcgacagacg ttgcagcgcg tggtttggat atttcaggtg tgacccatgt ctacaactac
    23701 gatattccac aagatcctga gagttatgtt caccgtatcg gtcgtacagg tcgtgctggt
    23761 aagtcaggtc aatctattac ttttgttgct ccaaacgaaa tgggttacct tcaaatcatt
    23821 gaaaacttga ctaagaaacg catgaaaggt ctcaaacctg caagtgtaga agaatccttc
    23881 caatcaaaaa aacaggtagc tctcaagaaa atcgaacgtg attttgcaga tgaaaccatt
    23941 cgtgccaact ttgagaaatt tggtaaggat gctcgcaaat tggctgctga gtttactcca
    24001 gaagaattgg caatgtatat cttgagtctg acagtccaag acccagatag ccttccagaa
    24061 gtggagattg cacgtgaaaa accactaccg tttaaaccat caggtaatgg tttcggtggt
    24121 aaagctaagg gaggtcgtgg aggccgtcgt ggggacgacc gtcgagagcg tgatcgtcgt
    24181 ggcaatggtc gccgtgatga gttcaaaaaa ggaagtcgtg gcaacgatcg ttttgataag
    24241 gaaaaacgtt accgtaagga taataaaaaa ccacgcaata ctttaagcga aaagcaaaca
    24301 ggctttgtta ttcgtaacaa aggtgataaa taagaaaaag cggttcaaaa tgaatcgctt
    24361 ttttatataa ggagaccgaa tgtgaaaaag acaaataaaa ttattatatt atcttttata
    24421 atattatgat acacaaaaat aggcgacttg tcaatagaaa aggaacaatt taattaaaaa
    24481 tattctggtt tttaattaaa ttgtaagtca atgcaagtca ataaggaatt ttcagataat
    24541 tattgaaaaa gagacgtttt tatgttataa tgatagcgat taaattcgag gtgaaaaatg
    24601 gcacatttat tagaaaaaac tagaaaaatt acatcaattt tgaaacgctc agaggagcag
    24661 ttgcaggatg agctccctta caacgctatt acgcgtcagt tagcggatat tattcattgc
    24721 aatgcctgca ttatcaatag taagggacgt ctgcttggct attttatgcg ttataaaaca
    24781 aatacagatc gcgtagagca attcttccaa actaagattt tcccagatga ctacgttcaa
    24841 ggggctaata tgatttacga aacagaagca aacttgcctg ttgagcatga tatgagtatt
    24901 ttccctgttg agagtagaga tgattttcca gatggcttga cgactattgc accgattcat
    24961 gtatcgggga ttcgccttgg ttctttgatt atttggcgta atgataaaaa attcgaagat
    25021 gaggacttgg ttcttgttga gattgccagt accgttgttg ggattcagct tcttaacttc
    25081 caacgtgaag aagatgagaa aaatattcgt cgtcgtactg ctgtcaccat ggcggtcaat
    25141 accctttctt actccgaact ccgtgctgtt tcagcaattt taggggaatt aaatggaaat
    25201 gaagggaagt tgactgcgtc agtgattgca gatcgtatcg gaatcactcg ctctgtgatt
    25261 gtcaatgctc ttcgtaaact tgagtctgcg gggattattg aaagtcgctc acttggaatg
    25321 aagggaacct atcttaaggt cttgatttca gatatttttg aagaagtgaa gaaaagagat
    25381 tactaatgac aaaggcttta atttcgattg attatacaga agattttgtt gctgatagtg
    25441 ggaagctgac agcaggtgct ccagctcagg cgatttcgga tgccatcagc aaggtaactc
    25501 gattagcttt tgaacgagga gattatatct tctttactat tgatgctcat gaagaaaacg
    25561 attgtttcca tccggaaagt aagttatttc ctcctcataa tctgattggg acgagtggac
    25621 ggaatttata tggagatttg gggatctttt atcaagagca tggttcagac agtcgtgtct
    25681 tttggatgga taaacgccat tactcagctt tttcagggac tgacctagat attcgtttga
    25741 gagagcgtag agtgtctacc gttatcttaa caggtgtctt gacggatatc tgtgtcctac
    25801 atacagctat agatgcctat aatctaggat atgacatcga gattgttaaa ccagctgttg
    25861 cttccatctg gcctgaaaat catcaatttg ccctaggtca tttcaaaaat acactcggag
    25921 ctaagttagt agatgaaaat ctaaatgaac tttctgagta acctggttgg tgaaaatgaa
    25981 aaaatttgaa aaaaagtgtt gacaagcatc ccaaaagttg atatactagt aaagtaatcg
    26041 atgcggggat ggcggaattg gcagacgcgc aggactaagg atcctgtgac cgctttaggt
    26101 cgtgagggtt caagtccctc tctccgcata ggaaaagagt tagctaaagc tagctctttt
    26161 gtatttctct aaaaaattag aatccagtca aatgtagtgg agaatcagcg aaaatatggg
    26221 aaaactgatc cttgtttctg cttttgtcat agaagaataa taaagattat cttcttcaaa
    26281 ttctccgata ttctctaaag ttttgtgcaa gttgcacaga acttttttat ttttttggtc
    26341 atcttgccat agaaatataa agcgttttca tctataatat aattatcaaa agacaaaagg
    26401 agttcacctc atggtagaat tgaatcttaa aaatatttac aaaaaatatc caaacagcga
    26461 acactattca gttgaagatt tcaacttgaa catcaaagat aaagaattta tcgttttcgt
    26521 aggaccttca ggatgtggta aatcaactac actccgtatg attgctggtc ttgaagacat
    26581 cacagaaggt actgcatcta tcgatggcgt ggttgtcaac gacgtagctc caaaagaccg
    26641 tgatatcgcc atggtattcc aaaactacgc tctttaccca cacatgactg tttatgacaa
    26701 catggctttc ggtttgaaat tgcgtaaata cagcaaagaa gacattaaca aacgtgttca
    26761 agaagcagct gaaatacttg gattgaaaga attcttggaa cgtaaaccgg ctgacctttc
    26821 aggtggtcaa cgtcaacgtg ttgccatggg gcgtgcgatt gtccgtgatg cgaaagtatt
    26881 cttgatggac gaacctttgt caaacttgga tgccaaactt cgtgtatcaa tgcgtgctga
    26941 aatcgctaaa attcaccgtc gtatcggagc tacaactatc tatgtaactc acgaccaaac
    27001 agaagcgatg acacttgcag accgtatcgt tattatgtca gctactaaga accctgctgg
    27061 tacaggtact atcggacgtg tagaacaaat cggtactcct caagaagttt acaaaaatcc
    27121 agttaacaaa ttcgttgcag gattcatcgg aagcccagct atgaacttca tcaacgtgaa
    27181 attggttggt agcgaaattg tttctgacgg tttccgtttg aaagtgccag aaggagcatt
    27241 gaaagttctt cgtgaaaaag gctacgaagg aaaagaattg atctttggta tccgtccaga
    27301 agacgtgaat gcagaacctg ctttccttga aacattccca gactgtgttg taaaagcgac
    27361 tatctctgta tcagaactgc ttggttcaga atctcacctt tactgccaag ttggtaaaga
    27421 cgagtttgtt gcaaaagttg atgctcgtga ctacttgcaa acaggtgcaa cagttgagct
    27481 tggatttgac ttgaacaaag cacacttctt cgatgtagaa actgaaaaaa caatctacta
    27541 agaataaata aaattcaaag cactacaaga aaagatatct ctttatcaat tgtagtggag
    27601 agatatcagt taatctaggg agagaaacaa aatgcttctc tcctttttgc tagggaagtc
    27661 atattataca tctatattgt gatgctcttt atactcttcg aaaatctctt caaaccacgt
    27721 caacgtcgcc ttgccgtacg tatgattact gacttcgtca gttttatctg caacctcaaa
    27781 gctgtacttt gagtagctta cggctagttt cctagtttgc tctttgattt ccattgagta
    27841 ttatttgtgg gtaccatcta caagtgaagc tatatgcgta aactacgtga gcaattgaat
    27901 tcgaactaga gaggtaataa taaatttatg ctatagttat ggtgacttgt atgcttttga
    27961 ttctagttta tcaaataata gattagaatt gtcagataat atcattttgt gtcattttgt
    28021 gttataatga agaaaaaaca gaggtgttca aatgtcagaa gcaggtcata agtttttagc
    28081 aaaattgggg aaaaaacgct tacgtccagg tggaaagcgt gccacagatt ggttaattgc
    28141 agaaggagga ttttcaaaag aaaagagaat actagaggtt gcgtgtaata ggggaactac
    28201 agcaattgag ttggcacagc gttttggttg caagataact gctgttgata tggatgctca
    28261 agctttagaa gtggctaaaa aatctgctgg aacggcaggt gttgctcatt taatcagttt
    28321 tgaaagagca aatgcaatga aacttcctta tcaagatgct agttttgata ttgttataaa
    28381 tgaagctatg ctgactatgc aagccgatca agctaagaaa aaatgtgtaa tggaatatct
    28441 aagggtatta aaacctggag gtcttctctt gacacatgat gtgcttctta aggaagctaa
    28501 agagtctatc agacaggaat tatcacaagc aattcatgta aatgtaggtc ctttaactca
    28561 agatggttgg gaacaggtga tgatagaatc aggttattgt gatgtgaaag tattgactgg
    28621 tgaaatgaca ttaatgaaat tatcgggtat gatttatgac gaaggttggc taggaacttt
    28681 gaaaatttgt gtaaatgctt gtaaaaagga gaatagaaag cagtttttaa ctatgtataa
    28741 aatgtttgct aagaataaac agaaattggg ctttattgcg atggctagtt ataaatcgtc
    28801 aaaacgttag ataattattg aagttaactt ttcctttttt ctttcttaaa aaatatgcta
    28861 taatagagag taaaaaactt tgaaagaaag aaaaagatga atttaaaaga ttacattgca
    28921 acaattgaaa attatccaaa ggaaggcatt accttccgtg atattagtcc tttgatggct
    28981 gatggaaatg cttatagcta cgctgttcgt gaaatcgttc agtatgctac tgacaagaaa
    29041 gtcgacatga tcgtgggacc tgaagctcgt ggatttatcg tgggttgtcc agttgccttt
    29101 gagttgggaa ttggttttgc gcctgttcgt aagccaggta aattgccacg cgaagttatt
    29161 tctgctgact atgaaaaaga gtacggtgtc gataccttga ctatgcacgc ggatgccatt
    29221 aagccaggtc aacgtgttct tattgtagat gaccttttgg cgacaggtgg aactgttaag
    29281 gcaactatcg agatgattga aaaacttggt ggtgttatgg caggttgtgc cttccttgtt
    29341 gaattggatg aattgaacgg ccgtgaaaaa attggtgact acgattacaa agttcttatg
    29401 cattattaat gaaaacagtc cctagggctg ttttctctac actaggatat aaaaatagac
    29461 tataactagt tagagaaaaa ctataattga aaactatatc ttcttacagt ataataaaag
    29521 gactaagtgt ttgagatttg tcttcaaaca tatgcaatta ttcctgaaag agtacagtta
    29581 ggagagggtt atgccgattc gaattgataa aaaattgcca gctgttgaga ttttacggac
    29641 agagaatatc tttgtcatgg atgatcaacg tgctgcccac caagatatcc gtcctttgaa
    29701 gattttaatt ttaaatctca tgccacagaa aatggtcaca gagacccagt tgttgcgcca
    29761 cttggctaat acacccctac aactggatat tgattttctc tatatggaga gccaccgttc
    29821 taaaacaact cgttcagagc acatggagac cttctataaa acttttcctg aagtcaagga
    29881 tgagtatttt gatgggatga tcatcacggg tgctccagtt gagcatttac catttgagga
    29941 agtggactat tgggaggaat ttagacagat gcttgagtgg tctaagactc atgtctattc
    30001 gacccttcat atctgttggg gggctcaggc tgggctttat ctgcgctatg gtgtagaaaa
    30061 ataccagatg gacagtaagc tatcaggtat ttatcctcag gacaccctaa aagagggtca
    30121 ccttctattt agaggctttg atgatagcta tgtatcccct cattcacggc acacggagat
    30181 ttctaaggaa gaggtcttaa acaagaccaa tctcgagatt ttatcagaag gacctcaggt
    30241 tggggtttct attttggcca gtcgtgattt acgagaaatt tatagttttg gtcatttgga
    30301 gtatgaccgt gataccttgg caaaagagta ttttcgagat cgtgatgcag gtttcgaccc
    30361 acatattcca gaaaattact ttaaagatga tgatgttaat caggtacctt gtctttgttg
    30421 gtcttcatct gcagccctct ttttcagtaa ttgggtaaac catgcggtct atcaggagac
    30481 gccttttgac tggagaaaga tagaagatga tgcatctgca tataggtatt tataagagga
    30541 attatgacat atttagacgc ttttaaatca ggtaccttgg ttttaccgag tgccctgctc
    30601 ttgcatttta aggaactctt tccttctagc gacgattttc tggtttggca atttttctat
    30661 ttgcaaaata cgacaggctt agaagaaata tcgccaagcc agattgctga aaggattggc
    30721 aaggaaattt cggatgtcaa ccagtccatt tctaatctga cggaaagggg actgctccag
    30781 tatcgtacta tcgaattaaa tggcgaaatt gaattgctct ttgatgctag tttggccttg
    30841 gaacgtttgg atgacctgtt tggagcagtt cattcaagtt cagaccagct aacaccacaa
    30901 aaccagctca aggatttggt ggaaaccttc cagcaggagt tgggacgatt gttgacgcct
    30961 tttgagattg aggatttgac caagacacta aaggaagatg gaaccagtgc tgacttgatt
    31021 aaggaggctc ttcgtgaagc tgttttgaac ggaaaaccaa actggaagta cattcaggcg
    31081 attttgagaa actggcgcca tgaaggaatc aagagtgtgg ctcaaattga ggccaagaga
    31141 gcagaaagag aagcaagcaa tcctcagttg acacaggtat ctgcagattt cataaatgcc
    31201 atggatctct ggaaggatta atccatgcaa gtaggcttga aatccgagta agatttgcaa
    31261 gctgtgtata attgtgatag aataaataga aaataaattg aaaaaagagg tatgtgaaat
    31321 gtcacgtaaa ccatttatcg ctggtaactg gaaaatgaac aaaaatccag aagaagctaa
    31381 agcattcgtt gaagcagttg catcaaaact tccttcatca gatcttgttg aagcaggtat
    31441 cgctgctcca gctcttgatt tgacaactgt tcttgctgtt gcaaaaggct caaaccttaa
    31501 agttgctgct caaaactgct actttgaaaa tgcaggtgct ttcactggtg aaactagccc
    31561 acaagttttg aaagaaatcg gtactgacta cgttgttatc ggtcactcag aacgccgtga
    31621 ctacttccat gaaactgatg aagatatcaa caaaaaagca aaagcaatct ttgcgaacgg
    31681 tatgcttcca atcatctgtt gtggcgaatc acttgaaact tacgaagctg gtaaagctgc
    31741 tgaattcgta ggtgctcaag tatctgctgc attggctgga ttgactgctg aacaagttgc
    31801 tgcctcagtt atcgcttatg agccaatctg ggctatcggt actggtaaat cagcttcaca
    31861 agatgatgca caaaaaatgt gtaaagttgt tcgtgacgtt gtagctgctg actttggtca
    31921 agaagtcgca gacaaagttc gtgttcaata cggtggttct gttaaacctg aaaatgttgc
    31981 ttcatacatg gcttgcccag acgttgacgg tgcccttgta ggtggtgcgt cacttgaagc
    32041 tgaaagcttc ttggctttgc ttgactttgt aaaataatca gtaagtagca aaagctaggt
    32101 ggaacagcat tcagatgtct gttacatttt ttataggaga ggaaagattg aaaacaaaaa
    32161 ttggattagc aagtatctgt ttactaggct tggcaactag tcatgtcgct gcaaatgaaa
    32221 ctgaagtagc aaaaacttcg caggatacaa cgacagcttc aagtagttca gagcaaaatc
    32281 agtcttctaa taaaacgcaa acgagcacag aagtacagac taatgctgct gcccactggt
    32341 atggggatta ttatgtaaag gatgatggct ctaaagctca aagtgaatgg atttttgaca
    32401 actactataa ggcctggttt tatattaatt cagatggtcg ctactcgcag aatgaatggc
    32461 atggaaatta ctacctgaaa tcaggtggat atatggccca aaacgagtgg atctatgaca
    32521 gtaattacaa gagttggttt tatctcaagt cagatggggc ttatgctcat caagaatggc
    32581 aattgattgg aaataagtgg tactacttca agaagtgggg ttacatggct aaaagccaat
    32641 ggcaaggaag ttatttcttg aatggtcaag gagctatgat gcaaaatgaa tggctctatg
    32701 atccagccta ttctgcttat ttttatctaa aatccgatgg aacttatgct aaccaagagt
    32761 ggcaaaaagt gggcggcaaa tggtactatt tcaagaagtg gggctatatg gctcggaatg
    32821 agtggcaagg caactactat ttgactggaa gtggtgctat ggcgactgac gaagtgatta
    32881 tggatggtgc tcgctatatc tttgcggcct ctggtgagct caaagaaaag aaagatttga
    32941 atgtcggctg ggttcacaga gatggtaaac gctatttctt taataataga gaagaacaag
    33001 tgggaaccga acatgctaag aaaatcattg atattagtga gcacaatggt cgtatcaatg
    33061 attggaaaaa ggttattgat gagaacgagg tggatggtgt cattgttcgt ctaggttata
    33121 gcggtaaaga agacaaggaa ttggcgcata acattaagga gttaaaccgt ctgggaattc
    33181 cttatggtgt ctatctctat acctatgctg aaaatgagac cgatgctgag aatgacgcta
    33241 aacagaccat tgaacttata aagaaataca atatgaacct gtcttaccct atctattatg
    33301 atgttgagaa ctgggaatat gtaaataaga gcaagagagc tccaagtgat acagacactt
    33361 gggttaaaat catcaacaag tacatggaca cgatgaagca ggcgggttat caaaatgtgt
    33421 atgtctatag ctatcgtagt ttattacaga cgcgtttaaa acacccagat attttaaaac
    33481 atgtaaactg ggtagcggcc tatacgaatg ctttagaatg ggaaaaccct tattattcag
    33541 gagaaaaagg ttggcaatat acctcttctg aatacatgaa aggaatccaa gggcgcgtag
    33601 atgtcagcgt ttggtattaa gcgatgattt gaaagaggga tgtgatagta gcaccctctt
    33661 tttctttgtt ttatgatagt tcatcctcga gtaaattcaa gatcttgctt ggaaatgaag
    33721 cttatatagt agattgaata tagacaaata ccttgtgatt ggtaaaacat tttagaaatt
    33781 catttacctt tcctaatcga cttggtttca tcttatttca atctattata gtattgggga
    33841 atttcttcaa accacatcag cttggtcagt tctacctgcg acctcaaaac ttgtgctttg
    33901 gtcaaactgg gtttagtttc ctagtttgct gatggatttc cattgactat aagcatccaa
    33961 ccctcttttt gtcttctaaa gaattcttaa attatcagtc tattgcaact tttctcatat
    34021 aagttctttg tcttgctatt ggttttcctt agtagtatac taaggtagta atcattaaga
    34081 agtggttaca aaaaataatg aatgaggtaa agaaaatggt agaattgaaa aaagaagcag
    34141 taaaagacgt aacatcattg acaaaagcag cgccagtagc attggcaaaa acaaaggaag
    34201 tcttgaacca agctgttgct gatttgtatg tagctcacgt tgctttgcac caagtgcact
    34261 ggtatatgca tggtcgtggt ttccttgtat ggcatccaaa aatggatgag tacatggaag
    34321 ctcttgacgg tcaattggat gaaatcagtg aacgcttgat tacactcggt ggaagcccat
    34381 tctctacatt gacagagttc cttcaaaata gtgaaatcga agaagaagct ggtgaatacc
    34441 gtaatgttga agaaagcttg gaacgtgttc ttgttatcta ccgttacttg tcagaacttt
    34501 tccaaaaagg tttggatgtc actgatgaag aaggtgacga tgtgacaaac ggtatctttg
    34561 caggcgctaa aactgaaaca gataaaacaa tttggatgct tgcagccgaa cttggacaag
    34621 cacctggttt gtaagaaata aggaaacata gataaatctg taggaaattt cttacagatt
    34681 ttttctattt tgtaagctat tccaaaccag tctttttttg agttttttga taaaatagta
    34741 ctatcagtga aaaggatgga agcatgacta agaaaatcgt agctatttgg gcccaggata
    34801 aagagggtgt gattggtaag gaaaatcgtc tgccttggca tttgccagca gaattgcagc
    34861 actttaaaga aacaactctg aatcatgcta tcttgatggg gcgtgtgacc tttgatggga
    34921 tggggcgtcg cttgcttcca aaacgggaaa ccctgatttt gacgcgtaat ccggaagaaa
    34981 agatagatgg ggttgctact tttcaggacg tccagtctgt tcttgattgg tatcaggctc
    35041 aagaaaagaa tctctacatt atcggtggga agcaaatttt tcaggctttt gaagcttacc
    35101 ttgatgaagt gattgtcact cacattcatg ctcgggtgga aggagatacc tatttccctg
    35161 gagagcttga cttgtctctt tttgagacag tttcaagcaa attttacgcc aaagatgaga
    35221 agaatcctta tgattttacc atccaatacc gcaagagaaa ggaagtctaa tggaacggag
    35281 tttatttggt ctattcacgg cttttctgtg tttcatctgc tttctggcag gagcacaagc
    35341 ttttcgtaaa aagcgttacg ggctttctat cctcctttgg ttaaatgcct ttaccaatct
    35401 ggtcaatagt attcatgctt tttacatgac cttattttag atagaatgac agtatagaat
    35461 ggaaggaaat catgtctaca aatagaaaaa atgatatgat ggtttattgc tcattttgtg
    35521 gcaaaaacca agaagaagta caaaaaataa ttgctggcaa caatgctttt atttgtaatg
    35581 aatgcgtgga gttagctcag gaaatcattc gagaagaatt ggttgaggaa gtcttggcag
    35641 acttgtctga ggtgccaaaa ccaattgaac tcctccatat cttgaaccac tatgtaattg
    35701 gtcaagatcg tgccaagcgt gccttggcag tggcggttta taatcactac aaacgcatca
    35761 atttccacga tacacgcgaa gagtcagaag atgtggattt gcagaagtca aacattttga
    35821 tgattggccc aactggttca gggaaaactt tccttgccca gaccttggct aagagcttga
    35881 atgtgccttt tgctattgcg gatgcgacag ctctgacgga ggctggttat gtgggtgagg
    35941 atgtggaaaa tatcctcctc aaactcttgc aggttgctga ctttaacatc gaacgtgcag
    36001 agcgtggcat tatctatgtg gatgaaattg acaagattgc caagaagagt gagaatgtgt
    36061 ctatcacacg tgatgtttct ggtgaagggg tgcaacaagc ccttctcaag attatcgagg
    36121 gaactgttgc tagcgtgccg cctcaaggtg gacgcaaaca tccacaacaa gagatgattc
    36181 aagtggatac aaaaaatatc ctcttcatcg tgggtggtgc ttttgatggt attgaagaaa
    36241 ttgtcaaaca acgtctgggt gaaaaagtca tcggatttgg tcaaaacaat aaggcgattg
    36301 acgaaaacag ctcatacatg caagaaatca tcgctgaaga cattcaaaaa tttggtatta
    36361 tccctgagtt gattggacgc ttgcctgttt ttgcggctct tgagcaattg accgttgatg
    36421 acttggttcg catcttgaaa gagccaagaa atgccttggt gaaacaatac caaaccttgc
    36481 tttcttatga tgatgttgag ttggaatttg acgacgaagc ccttcaagag attgctaata
    36541 aagcaatcga acggaagaca ggggcgcgtg gacttcgctc cattatcgaa gaaaccatgc
    36601 tagatgttat gtttgaggtg ccgagtcagg aaaatgtgaa attggttcgc atcactaaag
    36661 aaactgttga tggaacggat aaaccgatcc tagaaacagc ctagaggtga ctatggaact
    36721 taatacacac aatgctgaaa tcttgctcag tgcagctaat aaatcccact atccgcagga
    36781 tgaactgcca gagattgccc tagcagggcg ttcaaatgtt ggtaaatcca gctttatcaa
    36841 cactatgttg aaccgtaaga atctcgcccg tacatcagga aaacctggta aaacccagct
    36901 cctgaacttt tttaacattg atgacaagat gcgctttgtg gatgtgcctg gttatggcta
    36961 tgctcgtgtt tctaaaaagg aacgtgaaaa gtgggggtgc atgattgagg agtacttaac
    37021 gactcgggaa aatctccgtg cggttgtcag tctagttgac cttcgtcatg acccgtcagc
    37081 agatgatgtg cagatgtacg aatttctcaa gtattatgag attccagtca tcattgtggc
    37141 gaccaaggcg gacaagattc ctcgtggtaa atggaacaag catgaatcag caatcaaaaa
    37201 gaaattaaac tttgacccaa gtgacgattt catcctcttt tcatctgtca gcaaggcagg
    37261 gatggatgag gcttgggatg caatcttaga aaaattgtga ggaaaagaaa atggcaaaaa
    37321 caattcatac agataaggcc ccaaaggcta tcgggcccta tgttcaagga aaaatcgttg
    37381 gcaacctttt gtttgctagc ggtcaagttc ccctatcccc tgaaactggg gaaattgtag
    37441 gagagaatat ccaagaacag acagagcaag tcttgaaaaa catcggtgct attttggcag
    37501 aagcaggaac agactttgac catgttgtca aaacaacttg tttcttgagc gatatgaacg
    37561 actttgttcc ttttaatgag gtttaccaaa cggccttcaa agaggaattc ccagctcgtt
    37621 cagctgtgga ggtagctcgt cttcctcgtg atgtaaaagt cgaaattgaa gtcatcgcag
    37681 agattggata agctagttga agtttggtgt tgccaaactt cttttgatat aaggagaaaa
    37741 agatgacaaa gaaacaactt cacttggtga ttgtgacagg gatgagtggc gcagggaaaa
    37801 ctgtagccat tcagtccttc gaggatctag gttatttcac cattgataat atgccgccag
    37861 ctctcttgcc taagtttttg cagctggttg aaattaagga agacaatcct aagttggcct
    37921 tggtagtgga tatgcgtagc cgttctttct tttcagagat tcaagctgtt ttggatgagt
    37981 tggaaaatca agatggtttg gatttcaaaa tcctcttttt ggatgcggct gataaggaat
    38041 tggtcgctcg ttacaaggaa accagacgga gtcacccact agcagcagac ggtcgtattt
    38101 tagatggaat caagttggaa cgtgaactct tggcaccttt gaaaaatatg agccaaaatg
    38161 tggtggatac gactgaactc actccacgtg agctgcgcaa aacccttgca gagcagtttt
    38221 cagaccaaga acaagcccag tctttccgta tcgaagtcat gtctttcgga tttaagtatg
    38281 gaatcccgat tgatgcggac ttggtctttg atgtccgttt cttgccaaat ccctattatt
    38341 taccagaact gagaaaccaa acgggtgtgg atgaacctgt ttatgattat gtcatgaacc
    38401 atcctgagtc agaagacttt tatcaacatt tattggcctt gattgagccg attctgccaa
    38461 gttaccaaaa ggaaggtaag tccgttttga ccattgccat gggatgtacg ggtggacaac
    38521 accgtagtgt ggcatttgct aaatgcttgg cgcaggactt atccaagaat tggtctgtta
    38581 atgaagggca tcgcgacaaa gaccgcagaa aggaaacggt aaaccgttca tgagaaaacc
    38641 aaagataacg gtgattggtg gagggactgg aattcccgtc attctaaaaa gtctgcggga
    38701 aaaagatgtg gaaatcgcag ctatcgtgac ggtggcagat gatggtggtt cttcaggtga
    38761 actccgaaaa aatatgcaac agttgacacc gccaggtgat cttcgtaatg tccttgtggc
    38821 catgtcggat atgcctaagt tttatgagaa ggtctttcag tatcggttct ctgaggatgc
    38881 cggagccttt gctggccatc cattgggaaa tctcatcatt gctggcttgt cagaaatgca
    38941 gggttcaacc tataatgcca tgcagttatt gagcaaattt ttccatacaa cagggaaaat
    39001 ttatccttcc agtgaccatc ctttgaccct tcatgcagtc tttcaggatg ggacagaagt
    39061 ggctggagag agtcatattg tagaccatcg aggcataatt gacaatgtct atgtgaccaa
    39121 tgccctaaac gatgatacgc ctctggccag ccgtcgagta gtgcagacca tccttgaaag
    39181 tgacatgatt gtcctagggc caggttccct ctttacctct attttgccca atatcgtgat
    39241 taaggaaatt gggcgggctc ttttggaaac taaggcagaa attgcctatg tctgcaatat
    39301 catgacccaa cgtggggaga cggaacactt tacagatagc gaccacgtgg aagtcttgca
    39361 tcgtcacctt ggtcgccctt ttatcgacac tgtcttggtg aatattgaaa aagtgcctca
    39421 ggaatacatg aattccaacc gttttgatga atacttagtg caagtggaac acgattttgt
    39481 aggtctttgt aagcaagttt cgcgcgtgat ttcatctaac ttccttcgtc tggaaaatgg
    39541 cggtgccttc cacgatggag atttgattgt ggacgagttg atgcgcatta tacaggtgaa
    39601 aaaatgagtt tcacagtagc agtaaaagaa gaaatcctgg gccaacacca cctgagccgg
    39661 catgaattat ctgccattat caaaatgtct ggtagcatcg gtctctcgac ttcgggcttg
    39721 actttgtcag ttgtgacaga aaatgccaaa ctggcccgtc acctctatga gtcctttctc
    39781 catttctatg aaatcaaatc tgaaattcgt cactaccaac ggagcaatct tcgcaagaat
    39841 cgcgtctata ccgtttttac agatgaaaag gtgcaggatt tgttaagtga tttgcacttg
    39901 gcagactctt tctttggcct ggaaacaggt attgatgagg cgattttatc ggacgaggaa
    39961 gcaggtcgtg cctatctctg tggcgctttc ttggcaaatg ggagcattcg tgaccctgag
    40021 tcaggcaagt accagttgga aatcagttct gtttatctgg accacgcgca aggaattgcc
    40081 tcccttctcc aacagttttt actggatgcc aaggtgcttg agcgcaagaa gggggctgtg
    40141 acctatctcc agcgagcaga agacattatg gacttcttga tagtaatcgg agccatgcag
    40201 gcacgtgatg attttgagcg ggttaagatt ttgcgagaaa cccgtaacga cctcaatcgg
    40261 gctaataatg ccgagacagc taatatcgct cggacagttt ctgccagcat gaagaccatt
    40321 aacaatatca gtaaaatcaa agatatcatg ggcttagaaa atttgccagt agatttgcag
    40381 gaagtggcgc aactgaggat tcagcaccca gactactcta tccagcagtt agcagatagt
    40441 ctcagcaccc ctctgaccaa aagtggtgtc aatcacagac tcagaaaaat caataagata
    40501 gcggatgaat tataaaacca cgaatcctat gtgactcgtg gttctttttt ataaactagt
    40561 agagtgtttt ggttgtactt tttgttcagg gtcaatgtag ttgatagcgt tgttgacagc
    40621 agttggagct tctccgaggc ctgtcgcaat cagatcaatt tttccgtcat agtagcagca
    40681 gtcaccgata gcatagatac ctgcttggct ggattcctgt ttgctgttga cgataatctt
    40741 gtgacggttg aggtcgagcc cccagttttt aaggttaccg acagaagatt tgaaaccata
    40801 gttgacaaag aggtggtcta ggtcaatggt ttcagtttca tcagatttga cttttgtgat
    40861 ttcaagttta tcaagtgttt ttccatttcc aaggagttgg ctaggggcga atggtgtctt
    40921 gatggttaca gatgattctt gcaaggcttg aacactgtgt tccaaggcac ggaaattatc
    40981 tctgcggtga acaagggtag ttggtgcgat tttttcaaaa gccaaagccc aatccacagc
    41041 cgagtctccc ccaccaagaa tcgtcacttt cttaccagcg tattgctgaa tgttagaaac
    41101 gtggtagtgg atattttcat agccctcaac cccttcaagt tccagcggac gtggtttgaa
    41161 ggcaccgcca cccatagcga tgataactgt tttagtcagg tgacttcctt tagaagttgt
    41221 gatggcaaat tcttcttgtt tgtcaatctc aagaaccgtt tcattgagat gaataggggt
    41281 atcaaatcca tttagctgtt caatcaagcg gttagtcaac tcttctccag tcaggtttgg
    41341 gaagcctggt acgtctagga tttccttttc agggtagaga atagcaggtt gtccacctag
    41401 ctggggaaga gagtcgatga tttgaacctt ggcttggcgt aggtgggcat aaaaggctgc
    41461 aaaaagcccg acaggaccac cacccacaat ggtaatatca tagagttgag acatagtttc
    41521 tcctttgttt tttctagtca gtttatttta tcatattttt gttcaatgta aaatggacta
    41581 ggatagggaa aaaaatgcca gaaaatggta taataggacg aaagtgtttt gatagggagg
    41641 atatcaggga tgaattttca acaattatcc aatctgcaat attggaccag tttgtttgca
    41701 agtccatgga cgatagctat caatctgatt gatattttga ttgttgctta tattttatac
    41761 cattttacaa aagctattgc aggaaccaag attatgattt tggtacgtgg agttttggtg
    41821 tttattttag ctcaaatcct tgcaaatatg attggtttga ctacgatttc ttggttaatc
    41881 aatcaaatta ttacttatgg ggttattgcg gcggttgtta tcttctctcc agagattcgg
    41941 actggtttgg aacgtttggg aagagcgaca gatttttttt ccaatgcccc tattagtgct
    42001 gaggaacaga tgattcgtgc ctttgttaag tctgttgaat acatgagtcc tcgtaaaatc
    42061 ggggccttgg ttgctattca gcgtgtacgt accttgcagg agtatatttc gacaggaatt
    42121 cccttggatg ctaagatttc tgcagaactt ctcattaaca tttttattcc caacactccc
    42181 ctacatgatg gtgcggtgat tatcaaagaa gaacgtatcg ctgtgacgtc tgcctatctg
    42241 cccttgacaa aaaacacagg gatttccaag gaatttggga ccagacaccg ggcggctatc
    42301 ggtttatcag aagtctcaga tgccttgact tttgtcgtat cagaggaaac gggaggaatt
    42361 tcgataacct ataatggaag gtttaagcac aacctaacac ttgatgaatt tgaaacagaa
    42421 ttacgtgaaa tcttacttcc aaaagaggaa gtgggtctta gttttaaaga acgattgcta
    42481 ggaggatgga aacatgaaaa aaaatagttt atatatcata tcctcactct tttttgcttg
    42541 tgtcttattt gtctatgcta cggcgacgaa ttttcaaaac agtaccagtg ctaggcaggt
    42601 aaaaacggaa acctatacta atacagtaac aaatgtccct attgacatac gctataatag
    42661 tgataagtat tttattagcg gttttgcttc agaagtatca gtggtcttga ctggggcaaa
    42721 tcgcctatcg ctagctagtg aaatgcaaga aagtacacgt aaattcaagg ttactgctga
    42781 cctaacagat gccggtgttg gaacgattga agttcctttg agcattgaag atttacccaa
    42841 tgggctgacc gctgtagcga ctccgcaaaa aattacagtc aagattggta agaaggctca
    42901 gaaggataag gtaaagattg taccagagat tgaccctagt caaattgata gtcgggtaca
    42961 aattgaaaat gtcatggtgt cagataaaga agtgtctatt acgagtgacc aagagacatt
    43021 ggatagaatt gataagatta tcgctgtctt gccaactagc gaacgcataa caggtaatta
    43081 cagtggttca gtacctttgc aggcaatcga ccgcaatggt gttgtcttac cggcagttat
    43141 cactccgttt gatacaataa tgaaggtgac tacaaaacca gtagcaccaa gttcaagcac
    43201 atcaaattca agtacaagca gttcatcgga gacatcttcg tcaacgaaag caactagttc
    43261 aaaaacgaat taaaaataga aaaggatttt ataacaatgg gtaaatattt tgggactgat
    43321 ggagtccgtg gagaagctaa cctagaacta acaccagaat tagcctttaa actaggacgt
    43381 tttggaggct atgttcttag tcaacatgaa acggaagcgc cgaaagtctt tgtaggacgt
    43441 gacacacgta tttcagggga aatgctggaa tcggccttgg tggcaggtct cctttcagta
    43501 gggattcacg tatacaaact tggtgtcctt gcaacaccag cagtagctta cttggttgaa
    43561 actgaaggag caagtgccgg tgtcatgatt tctgctagcc acaacccagc ccttgataac
    43621 ggaatcaagt tctttggcgg tgatggcttc aaactagatg atgaaaaaga agcagaaatt
    43681 gaagccttgc tagatgctga ggaagacact cttcctcgtc caagtgcaga aggcttagga
    43741 attttggtag attatccaga aggcttgcgt aagtatgaag gataccttgt ttcaactgga
    43801 actcctcttg atggaatgaa ggttgccttg gatacagcta atggagcagc ttctaccagt
    43861 gcccgtcaaa tctttgcaga ccttggtgcc caattgacgg ttatcgggga aacaccagac
    43921 ggtcttaaca tcaaccttaa tgttggttca acacatccag aagcccttca agaagtggtc
    43981 aaagaaagtg ggtcagctat tggtttggcc tttgatggag acagtgaccg cttgattgct
    44041 gttgatgaga atggtgacat cgtcgatggt gacaagatta tgtacatcat cggaaaatac
    44101 ctttctgaaa aaggacaatt ggctcaaaat acaattgtga caactgttat gtctaacctt
    44161 ggtttccaca aggccttgaa tcgcgaaggt attaacaagg cagttactgc agttggtgac
    44221 cgctacgttg ttgaagaaat gagaaaatca ggctacaacc ttggtggtga acagtctggt
    44281 cacgttatct tgatggatta caataccaca ggtgatggtc aattatcagc agttcaattg
    44341 actaaaatca tgaaggaaac tggtaagagc ttatcagagt tggcggcaga agtaacgatt
    44401 tatccacaaa aattagttaa tatccgagtg gaaaacgtca tgaaggaaaa ggccatggaa
    44461 gtgccagcta tcaaggccat catcgagaag atggaagaag aaatggcggg gaacggccgt
    44521 atccttgttc gtccaagtgg aacagagccc ctcttgcgtg ttatggcaga agcgcctaca
    44581 acagaagaag tggactacta tgttgatacc atcacagatg tagttcgtgc tgaaattggg
    44641 attgactaaa tcctagaaaa ctagatgaaa ataaaaaatt atggtacaat agcttataat
    44701 attgtagccg atggagaaag acatgaatct taaacgtgaa caagaatttg ttagtcagta
    44761 tcattttgat gcgcgtaatt ttgaatggga aaatgaaaat ggagctcctg aaaccaaggt
    44821 ggatgtgaat ttccaattgc tccaacatga ccaagaaaat caagtgactt ccttgattgt
    44881 catcttgagc tttatgattg tatttgataa gtttgtcatt agtggaacaa tctcacaagt
    44941 aaaccatatc gatggccgta ttgtcaatga accaagtgaa ttgaaccaag aagaggtgga
    45001 aaccttggct cgtccatgtt tgaacatgct caatcgtttg acttacgaag tgactgaaat
    45061 tgcattagac ttaccaggaa ttaatttgga gttctaatat gaaattagct gttttcacag
    45121 attcctctgc ttatctcagt gaagagacct tgcaaagaga agacttgttt gtcttggata
    45181 ttcctgtcaa tattgatggt gaggaatatg tcgaaggcat caatctgtct gctgaggaat
    45241 tttaccaaaa aatggctcag gcttctgaat tgcctaagac cagtcaacca agtattgcca
    45301 agttagatga gatcttaact tcgctcaaag aacaaggcta tacacatgcc ttggggcttt
    45361 tcctatcttc tggaatttca ggtttttacc aaaatatcca gtatatggtc gatgactatg
    45421 agggcttaac cattgctttc ccagacactt tgattacaag tgctccccta ggcatcatgg
    45481 ttgaaagcgt ctttaattgg cgtgatcagg gcgatgattt tgccagcatt caggataagc
    45541 tagccattca aatcagccgt acgtcagcct ttatcatggt agatgacttg gatcatttgg
    45601 taaaaggtgg acgcctttca aatggggctg ccattttggg caatttgctt agcattaagc
    45661 caatccttta ttttaacgat caaggtgtga ttgaagttta cgaaaaagtt cgtactgaaa
    45721 agaaggccac caaacgctta attgaaatta tcaaggaaac aacggcttca ggtcaatacc
    45781 gggtcattgt cattcatggg aatgatcctg aaaaggctga agaattgcgt cagcatttgc
    45841 ttgattttgg cttgggttcg gatgtttcac ttgctacatt tggtagtgtc attggaacgc
    45901 acctaggagc aggaagtatt gctctgggtt atattccagt gatttagtag gcacttctag
    45961 gctagttaat cttagcaatt gaacacggac tacctgcctc ttgaaaaaag atgtctgtct
    46021 tgcctttcgt ggaaagtcag tgccattccc tatttttcat gggtggctaa gatcctttgt
    46081 attttgataa ttgaacacgc ctggaaccct gtgtgaaaaa gatagttctt ccaaggagta
    46141 gacacttctt gatcagaatt cctattttca ctttgtgttc ttacaggctt tgtatcttag
    46201 acaggagtag agatgagtat tcgagtaatt attgccggtt ttaagggaaa gatgggtcaa
    46261 gctgcttgcc agatggtctt gcctgattca gatttggact tggtagcagt tttggatcct
    46321 tttgagtctg agtcagaatg gcagggtatt cctgttttca aggataaggc tgatttagct
    46381 ggttttgaag cggatgtctg ggtagacttt actactccag ctgttgccta cgaaaataca
    46441 cgttttgctc ttgaaaatgg ctttgctcca gtagttggaa cgactggttt cacgagtgaa
    46501 gaaattgcag agttaaaaga attttctcgt gcccaagact tgggtggcct gattgcccct
    46561 aactttgcct tgggtgctgt cttactcatg caatttgcga cgcaggctgc caaatatttc
    46621 ccaaatgtgg agattattga gctccatcat gacaagaaaa aggatgctcc gagtggaaca
    46681 gccattaaaa cagctgagtt gatggcagag gttcgagagt ccattcagca aggcgcagca
    46741 gatgaggaag agctgattgc tggtgctcgt ggtgctgact ttgatggtat gcgcatccac
    46801 tcagttcgtt tgccaggttt ggtagcccat caggaagtca tctttggcaa tcaaggagaa
    46861 ggattgaccc tccgtcatga ctcctatgat cgcatctcct tcatgacagg agtcaatttg
    46921 ggaattaaag aagttgtcaa gcgtcatgag cttgtctatg gattagaaca cttattatga
    46981 gattaacgca aatgccttct gaatttcaga aggctttacc agtattagaa aaaattaaag
    47041 aagcaggctt tgaggcttat tttgttgggg gctctgttcg agatgccctt ctccatagcc
    47101 ctatccacga tgtggatatt gcgacgtctt cttatccaga agagaccaag cagatttttc
    47161 cgcgaacagc cgatatcgga atcgagcatg gaaccgtctt ggtcttagat ggggatgagg
    47221 agtatgaggt aacaaccttt cggacagagg atgtctatgt ggactatcgc agacccagtg
    47281 cggtttcctt tgtgcgctcg ctagaagaag acctcaaacg ccgtgatttc acagtcaacg
    47341 cctttgcctt ggatgagaca ggagaaatcg ttgacttgtt ccatggttta gaagatttgg
    47401 aaaagcaagt cttgcgagca gttggagtgg ctagtgagcg tttcaacgaa gatgctttac
    47461 ggattatgcg tggtttccgt tttcaggcta gtcttggttt tgcacttgag ccagaaacat
    47521 ttaaagctat gaagaccttg acgccgcttt tggagaacat ttctgtagag cgtaccttcg
    47581 tcgagtttga taaactcttg ctggctccat tttggagaag gggcttggct tccatgattg
    47641 agagtcaagc ttatgactat ctccctgata tggcatctag ccagaacaag ctcaacagac
    47701 tgtttgattt ggagactgat tttacttttg aatcctctga acaagcctgg gcggctttac
    47761 tatgggcttt ggagattgaa aatgcgcagt catttttgaa atcttggaag acctcacgcc
    47821 agtttgccaa gcaagttcag gatttgctga ttattttggc tctgcgtgaa aatggagaat
    47881 tgagcaagcg agattgttat cgctttgaca tagatttgct tttacaggct gaaaatcttc
    47941 gtcaagctcg aggaaaagaa gtcaacccac aagccatcac agaaaaatac caaagcttga
    48001 ctatccatga caagaaagag attcagatta atggcgatat tttgatcaag gaatatggct
    48061 atcagccagg cccagacttg ggagagattt taacagagat tgagtttgcc attgtcgatg
    48121 gagaattgga gaataatcgt gaagccatcc atgcttacct gagggagaaa aaatgagtga
    48181 ttttatcgtt gaaaaactaa gtaaatccgt tggtgacaag accgttttta gggatatttc
    48241 ctttattatc catgacttag acagaattgg tttaatcggt gtcaatggga ctggcaagac
    48301 cacccttttg gacgtccttt ctggtgtttc tggctttgat ggggatgtca gtcctttttc
    48361 agctaaaaat gattaccaga ttggttactt gactcaggat cctgattttg atgatagaaa
    48421 gacagttttg gatacggttc tatctagtga actcaaggaa atccagctca ttcgtgagta
    48481 tgaattgatt atgctcgact atagtgagga caagcaggcg cgtttggaac gtgtcatggc
    48541 agagatggac tctctccaag cttgggaaat cgagagtcag gtcaagaccg ttcttagcaa
    48601 attgggcatt caagacttat ctactcctgt tggggaattg tcaggtggtc tgagaagacg
    48661 ggtacagttg gcacaagtct tacttggcaa ccacgacctc ttgcttttgg atgagccgac
    48721 caaccatctg gatattgcga ttattgagtg gctgaccctc tttttgaaaa attctaagaa
    48781 gaccgtcctt tttatcactc acgatcgtta tttcttagac gctttgtcaa cacggatttt
    48841 cgagttggag cgagcaggct tgacagaata tcagggcaat taccaggact atgttcgcct
    48901 taaggcagaa caggacgagc gcgatgcaac tctacttcat aaaaaagaac aactctacaa
    48961 acaagaactg gcctggatgc gcagacaacc gcaggcacgt gcgaccaagc aacaagctcg
    49021 tatcaatcgt ttccatgatc tgaaaaagga agtttcaggc agtagtgctg agacagactt
    49081 gactatgaac tttgaaacca gtcggattgg gaagaaagtc atcgagtttc aggatgtttc
    49141 ctttgcctat gaaaataagc ccattttgca agattttaat ctcttggtac aggtcaaaga
    49201 tcgtatcgga atcgttgggg acaatggtgt tggaaaatca accctactta acctgattgc
    49261 aggaagtctt gagccgatag caggacaagt tgtgattggg gaaactgttc gcatcgccta
    49321 tttctctcaa caaattgagg gtttggatga aagcaagcgt gtgatcaatt acctgcagga
    49381 agtggcagag gaggtcaaga ccagtggtgg ttctacgact tccatcgctg agttgctgga
    49441 gcaattcctc ttcccacgtt cgacgcatgg gaccttgatt gagaaattat ctggtggaga
    49501 gaaaaaacgt ctttatctcc tcaaactgct cttggagaaa ccaaatgttc ttcttttaga
    49561 cgagccaacc aatgacttgg atattgcaac tttgacagtc ttagagaatt tcttgcaagg
    49621 ttttgcaggt cccgttttaa cagtcagtca cgaccgctat ttcttggata aggtagcgac
    49681 caagattctc gcttttgagg atggcaagat tcgtcctttc tttggtcatt acaccgacta
    49741 tcttgatgaa aaagcttttg aaacagatat ggccaatcaa gtgcaaaagg ccgaaaagga
    49801 aaaagtggtc aaggttcgag aagacaagaa acgcatgacc taccaagaaa agcaggagtg
    49861 ggcaagtatt gaaggtgata ttgaaacctt ggaaaaacgt atcgctgcta ttgaagagga
    49921 aatgcaggct aacggctctg actttggtaa gctggctact ctccaaaaag aattggatga
    49981 gaaaaatgaa gcactccttg aaaaatacga acgctatgag tatctcagtg aatttgatag
    50041 ttaagactat tcgaaaatca aagtgcaaac tatgtcggct tcacattgcc gactcaagta
    50101 cagcctgtga ccggcttcct aatttgcact ttgattttta ttgagtataa aagaatagaa
    50161 aaatcccgtc tcatgaaagg atttctctat tcttttttgg tttcttgatg aaagatattt
    50221 tgccaggttt cgtggcgacg ccagatactg gtatggatga taccatctaa ctcatagcag
    50281 atgagtttgg ttttttgact gatggaagta atttgaatgt ccttgatagc agaatttaac
    50341 tcttttttgg tcttataagc ctctttatcc atctgctctc catcttggcg aatatagaca
    50401 aaatcatcag tcaagagttc ttctagttgg tttccttggt caatcaattg ttcacgcata
    50461 agcaattttt tatagacatt gtctaaaatt tcatcctcag ggataggtgc tggttcgata
    50521 tggacatcgg tatcaaagac gccaaaacgc tcctccagca tagactcgac ctgatccgcg
    50581 atttcatggc tttcaaaaac agacaagtca ggattcatct ctagtgtaat atccaggtag
    50641 atgttgctac cgtaggtgcg acctctttgc gatttgacct tgctgatttt gggaatttcc
    50701 atgatagcct tttggtagtc ctcgagcagg cggtcgtcaa agccatctga aagactaaag
    50761 gaagactcga tgaagatatc ataggcagtc ttcaagataa agaaagtgat gatgatagca
    50821 accagtttat ccacaatcgg ataattgaaa ctactagcta ggatggcaat ggcggttcca
    50881 agtgaggtaa cagcgtcaga aagattgtcc ttagcagctg ccttcagcgc cttggagttg
    50941 gatttcttac tgaggcgagt attgtagaga tagaccacaa acataatcgc tgcagaaatg
    51001 attcctagag ttgcaccaag aggatcaatg accgtttctt cccgactgag aatcttttga
    51061 atggtatctc ttagaatatc gaaaccgaca tagaacatga tgatagaagt gatcaagctt
    51121 gccaaatctt caatcttcca atgaccaaaa cggtggtcac ggtctgcagg ctggcgcgcc
    51181 atccgaatcc cgattaagag ggccacattt ccaatgatgt ccgatacgtt attaaaacca
    51241 tcggccacca aactggatga atgaaggaga tgaccagctg ctaatttagc tgcagacaag
    51301 atcaaatagg tcgaaatact gataatggct ccacgctcag ctaacttgag atttgagata
    51361 gattgcttca ttcaacttcc tttctagtca catagcattc taccattata ctggttttca
    51421 aggaaaaatt caaataaggc agtttttact ctttgaaaac tccccaaaat ttggtataat
    51481 ggtactactc aagggagtag ctggcagaaa cctgtgatag tgtcgtcatt ccgaatttta
    51541 tgctgaaaag tatgctttcc ggccctatct taaacagcga gacttgttat gattaacagg
    51601 tctctttttg tttgtcgtga aaagatatga cagaaagaga gtctgttttg cacacaatgt
    51661 caaggaggag acacatgtca aaagaacaaa aacgccaagc gttttatact cagagccctg
    51721 aagaggtctt gcaggctgtg gatgcgaccg agcaaggttt gtcatcaagt gaggcggaaa
    51781 agcgccttgc cgaatttggg cacaatgaac tcgaagaagg cgagaaacga tcaatcttgg
    51841 tcaaattcat cgagcaattt aaggatttga tgattatcat cctagttgcg gcagcaatct
    51901 tgtcagtcgt gacttctggt ggggaagata tcgcagatgc cattatcatc ctagctgtgg
    51961 ttatcatcaa cgctgccttt ggtgtttacc aagaaggaaa agctgaagaa gctatcgaag
    52021 ccctcaaatc catgtctagt ccagttgccc gcgttcttcg tgatggacac atggcagaga
    52081 ttgactctaa agaattggta ccaggcgata tcgttgccct tgaagcaggt gacgtggtac
    52141 cagcggacct acgtttgcta gaagccaact ctcttaaaat tgaagaagca gccttgacgg
    52201 gtgaatctgt accagtcgaa aaagacttgt cagtcgagct tgcgacagat gctggtattg
    52261 gtgaccgtgt caacatggcc ttccaaaact caaacgtaac ctatggtcgt gggatgggtg
    52321 ttgttgtcaa tacaggtatg tacactgaag ttggtcatat tgctggtatg cttcaagatg
    52381 cggatgagac tgatacacca ctcaaacaaa acttgaacaa cctttctaag gtcttgacct
    52441 atgctatctt ggtcattgcc cttattactt ttgtagtggg tgtcttcatt caagggaaaa
    52501 atccacttgg tgagttgttg acttctgttg cccttgccgt tgcagccatt ccagaaggac
    52561 ttcctgctat cgttaccatc gttctttctc ttggtactca agttttggcc aaacgacatt
    52621 ccatcgttcg taagttgcca gcagttgaaa cacttggttc aactgaaatc atcgcttctg
    52681 ataagactgg tacgctgact atgaacaaga tgacagtcga aaaagtcttc tacgatgcgg
    52741 ttctacatga ctcagctgat gatattgaac taggtcttga aatgccacta cttcgttcag
    52801 ttgtcttggc caatgatacg aaaatcgatg tggaaggtaa cttgattggt gacccaaccg
    52861 aaacagcctt tatccaatat gccttggaca agggctatga tgtcaaaggt ttcttagaga
    52921 aatatcctcg tgtagctgaa ttgccatttg actctgaccg taagctcatg tcaacagttc
    52981 acccattgcc agatggtcgt ttccttgtag cagtcaaggg tgcgccagac caactcttaa
    53041 aacgttgtct tcttcgtgat aaggctgggg atattgctcc gattgatgag aaggttacaa
    53101 atctcattca tacaaacaat tctgaaatgg ctcatcaagc cttgcgtgtc cttgcaggtg
    53161 cttataagat tatcgatagt attccagaaa atctcacttc tgaagagctt gaaaatgatt
    53221 taatttttac tggtttgatt gggatgattg accctgaacg tcctgaagcc gctgaggctg
    53281 ttcgtgtggc taaggaagcg ggaatccgtc cgattatgat tacaggtgac caccaagaca
    53341 cagcggaagc cattgccaaa cgtttgggaa tcattgacgc aaacgataca gaaggtcacg
    53401 ttttaactgg tgctgaactc aatgaactgt cagatgaaga atttgaaaaa gtcgttggtc
    53461 aatactctgt ttatgcccgt gtgtctccag aacacaaggt tcgtatcgtc aaggcttggc
    53521 aaaaacaagg taaagtcgtt gccatgacag gtgacggtgt caatgacgcg ccagctctga
    53581 aaacagccga tatcggtatc ggtatgggaa tcactggtac agaggtttct aagggggctt
    53641 ctgatatgat tcttgcagat gataactttg cgactattat cgtcgcagtg gaagaaggac
    53701 gtaaggtctt ctcaaacatt caaaagacta ttcagtacct actttctgct aatactgctg
    53761 aagtattaac catcttccta tcaaccttgt ttggttggga tgtcttacag ccggttcatc
    53821 ttttgtggat caacttggta acggatacct tcccagctat cgctcttggt gttgaacctg
    53881 cggaacctgg tgtcatgaat cataaaccac gtggacgcaa ggcaagcttc ttctcaggtg
    53941 gtgttttgag ttctatcatt tatcaaggtg tactccaagc agctcttgtt atgagtgttt
    54001 atggccttgc gattgcttac ccagttcatg tgggtgacaa tcatgctatt catgcagatg
    54061 ccctaacgat ggcctttgca acccttggtt tgattcagct cttccatgcc tacaatgtca
    54121 agtctgtcta ccaatccatc ttgacagttg gcccattcaa gtctaagacc tttaactggt
    54181 ccatcttggt atcctttatc cttctcatgg caacaatcgt tgtagaaccg cttgaaggaa
    54241 tcttccacgt aaccaaacta gacttgtcac aatggggaat tgttatggct ggaagcttct
    54301 caatgattat catcgtcgaa atcgttaagt ttattcaacg caaactcggt tttgacaaga
    54361 atgcgattta agggaggttt cggatgtcag catatcaatt accgaccgta tggcaggatg
    54421 aagctagtaa tcaaggagct tttacggggc taaacagacc aacagcaggt gcccgtttcg
    54481 aacaaaactt gccaaaagga gaacaagctt ttcagcttta ttcactggga acaccaaatg
    54541 gtgtgaaggt tactatctta ttggaagaat tactagaagc tggttttaag gaagcggctt
    54601 acgacttgta taagattgct atcatggatg gggatcaatt cggctcagac tttgtgaagc
    54661 tcaatccaaa ttccaagatt ccagccttat tggaccagtc aggtactgaa aacgtaagag
    54721 tctttgagtc tgctcatatt cttctttacc ttgctgagaa atttggagcc tttttaccaa
    54781 gtaatcctgt ggaaaaggta gaagttttga attggctatt ctggcaagca ggtgcagcac
    54841 cttttctagg tgggggattt ggacatttct tcaattatgc tcctgaaaaa ttggaatatc
    54901 ctattaaccg ttttacgatg gaagtgaaac gccagttgga tttattggat aaggaattgg
    54961 ctcagaaacc ttatattgca ggcaatgact atacgattgc agatattgct atctggtctt
    55021 ggtatggaca gttagttcaa ggaaatcttt accaaggttc tgcaaaattc ttggatgcct
    55081 caagttatca aaatctagta aaatgggcag aaaaaattgc caatcgtcca gctgttaagc
    55141 gtggcttgga agtaacttat acagaaatta aatagcaaaa aagtcatctt cggatggctt
    55201 tttgctacag ttgagggaaa gggtttgcag ttatcggctt ttgtgctata attgctataa
    55261 tatagtaaaa agcaagagga gtgtgaggaa gtggaaaaga aaattgtgaa ggatatctta
    55321 tttttatctc aagtgtctca gccggcaagt caggaggacc tttatcttgc cagagatttg
    55381 caggatacac tcttagcaaa tcgtgatacc tgtgttggtc tagctgccaa tatgattggg
    55441 gtgcagaagc gcgtgattat ctttaatctt ggcttggttc ccgtggtcat gtttaaccca
    55501 gtgcttctgt cctttgaagg atcctatgag gcagaagaag gctgtttgtc cttggtaggt
    55561 gtgagatcaa ctaagcgtta tgaaaccaca aggcttgcct atcgtgacag caagtggcag
    55621 gaacagacca ttaccttgac aggcttccca gctcagattt gccagcatga gctggatcac
    55681 ttggaaggac gaatcattta ggaggaaagc aaatgaaacg aatagtcttt gaacttattt
    55741 ttatcgcaac gacctggtat atctttttac cgccccttaa cctgaccagc tgggaatttc
    55801 tcttcttcct ctgtgggcat ttgttagttg tggcaatctt atttggcttt ggcaagggga
    55861 taaaccttgt caaaacggtt catgtgcgcc acggtaaggc ggaagctgcc ttaaatcttg
    55921 agggtttcaa aatcaatcgg ttagggaaaa ttctgttagc ttcgattgga ggaattcttc
    55981 tcttggcagc tttggtttcc ttggtaactt ccagcatgtt tcaggctaaa aattatgcca
    56041 atgtagtcac ggttacggaa aaagacttta ctgaatttcc taggagtgac accagtaagg
    56101 ttcctatcct agatagaagt actgctgaaa aaattggaga ccgctacttg ggttccctaa
    56161 ccgataaggt gtcgcaatac gtagcggcag atacctatac ccaattgaca attgatggga
    56221 aaccttatcg ggtcacacca ctagaatatg cagaccctat caaatggttt aacaatcaag
    56281 ccaagggaat cggtgagtat atcaaggtgg acatggtaac tggaaatgcg gatttggtgg
    56341 acttgaagac accaatcaag tattcagact cggagtattt taaccgtgat gtcaaacgtc
    56401 acctgcgctt gaagtacccg actaaaatct ttaaaactcc atcttttgag gtggacgatg
    56461 agggcaatcc tttctatgta gcaacggttt accaaaagca atttggactt gcggttcctc
    56521 gtcctgcttc agtcattatc ttggatgcta caaatggaga aaccaaggaa tacagcttat
    56581 cagatgttcc agaatgggtg gacaggatct atccagcaga ggaaaccatt gagcaaatca
    56641 actacaacgg caagtacaag gacggtttct tgaatgccat gatttccaag aaaaacgtga
    56701 cccagactac caatggctat aattacttgt ctatcggtaa tgacatctat ctctacacag
    56761 gtgtgacgtc ggctaatgcg gatgagagta atcttggttt catccttgaa aatatgcgaa
    56821 caggagaaat tactaagtat agcttggctt ctgcgacaga agaatcagcc cgtgaatcag
    56881 cagaaggtgc tgttcaggag aaatcctaca aagcaacctt cccaatcctc atcaacctca
    56941 atgacaagcc tctctacatc atgggcttaa aggacaatgc tggcttggtc aaagagtacg
    57001 ccctggtaga cgcagtcgag taccaaaatg ttatcgttgc tactacagtg gaagagatgc
    57061 tcagcaagta tgccaataaa aacgaccttg aaattgacaa tgcaacgaca gaaagcatca
    57121 agggagtagt agcagacctc aaatcagctg ttatcaaggg agacactgtc tacttcttta
    57181 aagttgatgg caagatatac aaggtaaagg cttcagtatc cgatgacctt ccttaccttg
    57241 aaaatggtaa aaccttcgaa ggtcaagtag gaaaagacaa ttatctcaag acctttaaag
    57301 tacaataata ctcttcgaaa atctctagct ctatctgcaa cctcaaaaca gtgttttgag
    57361 cagcctgcgg
//
LOCUS       DAVLWA010000004        54478 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLWA010000004 DAVLWA010000000
VERSION     DAVLWA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.004"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            213..527
                     /locus_tag="VYN80_000226"
     CDS             213..527
                     /locus_tag="VYN80_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540204.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            537..743
                     /locus_tag="VYN80_000227"
     CDS             537..743
                     /locus_tag="VYN80_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540205.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            743..940
                     /locus_tag="VYN80_000228"
     CDS             743..940
                     /locus_tag="VYN80_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540206.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQGDQFVWLYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            943..1677
                     /locus_tag="VYN80_000229"
     CDS             943..1677
                     /locus_tag="VYN80_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1540207.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1689..2330
                     /locus_tag="VYN80_000230"
     CDS             1689..2330
                     /locus_tag="VYN80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1540208.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2348..2578
                     /locus_tag="VYN80_000231"
     CDS             2348..2578
                     /locus_tag="VYN80_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540209.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2930..4693
                     /gene="aspS"
                     /locus_tag="VYN80_000232"
     CDS             2930..4693
                     /gene="aspS"
                     /locus_tag="VYN80_000232"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1540210.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEAKDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4671..5612
                     /locus_tag="VYN80_000233"
     CDS             4671..5612
                     /locus_tag="VYN80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1540211.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5822..5902)
                     /locus_tag="VYN80_000234"
                     /pseudo
     CDS             complement(5822..5902)
                     /locus_tag="VYN80_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6038..7024)
                     /locus_tag="VYN80_000235"
     CDS             complement(6038..7024)
                     /locus_tag="VYN80_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1540212.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(7034..7834)
                     /locus_tag="VYN80_000236"
     CDS             complement(7034..7834)
                     /locus_tag="VYN80_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1540213.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(8095..8937)
                     /locus_tag="VYN80_000237"
     CDS             complement(8095..8937)
                     /locus_tag="VYN80_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1540214.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8939..10231)
                     /locus_tag="VYN80_000238"
     CDS             complement(8939..10231)
                     /locus_tag="VYN80_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1540215.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10342..11613)
                     /locus_tag="VYN80_000239"
     CDS             complement(10342..11613)
                     /locus_tag="VYN80_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1540216.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12163..13680
                     /gene="malQ"
                     /locus_tag="VYN80_000240"
     CDS             12163..13680
                     /gene="malQ"
                     /locus_tag="VYN80_000240"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1540217.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            13706..15964
                     /gene="glgP"
                     /locus_tag="VYN80_000241"
     CDS             13706..15964
                     /gene="glgP"
                     /locus_tag="VYN80_000241"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1540218.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(17009..17581)
                     /locus_tag="VYN80_000242"
     CDS             complement(17009..17581)
                     /locus_tag="VYN80_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540219.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTTIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18204..19052)
                     /locus_tag="VYN80_000243"
     CDS             complement(18204..19052)
                     /locus_tag="VYN80_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1540220.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            19204..19482
                     /locus_tag="VYN80_000244"
     CDS             19204..19482
                     /locus_tag="VYN80_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540221.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19484..21547
                     /locus_tag="VYN80_000245"
     CDS             19484..21547
                     /locus_tag="VYN80_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1540222.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21625..22881)
                     /locus_tag="VYN80_000246"
     CDS             complement(21625..22881)
                     /locus_tag="VYN80_000246"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1540223.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEESYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23023..25488
                     /locus_tag="VYN80_000247"
     CDS             23023..25488
                     /locus_tag="VYN80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1540224.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLASSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGATATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25553..26464
                     /locus_tag="VYN80_000248"
     CDS             25553..26464
                     /locus_tag="VYN80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1540225.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26694..27392
                     /gene="dapD"
                     /locus_tag="VYN80_000249"
     CDS             26694..27392
                     /gene="dapD"
                     /locus_tag="VYN80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1540226.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVSENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27460..28590
                     /locus_tag="VYN80_000250"
     CDS             27460..28590
                     /locus_tag="VYN80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1540227.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIHFKGKDGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGIFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHSQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28602..29141
                     /locus_tag="VYN80_000251"
     CDS             28602..29141
                     /locus_tag="VYN80_000251"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1540228.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            29125..29802
                     /locus_tag="VYN80_000252"
     CDS             29125..29802
                     /locus_tag="VYN80_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1540229.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(29874..30773)
                     /gene="galU"
                     /locus_tag="VYN80_000253"
     CDS             complement(29874..30773)
                     /gene="galU"
                     /locus_tag="VYN80_000253"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1540230.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTEQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(30795..31811)
                     /locus_tag="VYN80_000254"
     CDS             complement(30795..31811)
                     /locus_tag="VYN80_000254"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1540231.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            32023..32463
                     /locus_tag="VYN80_000255"
     CDS             32023..32463
                     /locus_tag="VYN80_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540232.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(32586..33002)
                     /locus_tag="VYN80_000256"
     CDS             complement(32586..33002)
                     /locus_tag="VYN80_000256"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1540233.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(33070..33720)
                     /gene="phoU"
                     /locus_tag="VYN80_000257"
     CDS             complement(33070..33720)
                     /gene="phoU"
                     /locus_tag="VYN80_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1540234.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(33735..34487)
                     /gene="pstB"
                     /locus_tag="VYN80_000258"
     CDS             complement(33735..34487)
                     /gene="pstB"
                     /locus_tag="VYN80_000258"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1540235.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(34489..35304)
                     /gene="pstA"
                     /locus_tag="VYN80_000259"
     CDS             complement(34489..35304)
                     /gene="pstA"
                     /locus_tag="VYN80_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1540236.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(35297..36160)
                     /gene="pstC"
                     /locus_tag="VYN80_000260"
     CDS             complement(35297..36160)
                     /gene="pstC"
                     /locus_tag="VYN80_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1540237.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(36278..37153)
                     /locus_tag="VYN80_000261"
     CDS             complement(36278..37153)
                     /locus_tag="VYN80_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1540238.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(37320..38651)
                     /locus_tag="VYN80_000262"
     CDS             complement(37320..38651)
                     /locus_tag="VYN80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1540239.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(38652..39359)
                     /locus_tag="VYN80_000263"
     CDS             complement(38652..39359)
                     /locus_tag="VYN80_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540240.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(39443..39919)
                     /locus_tag="VYN80_000264"
     CDS             complement(39443..39919)
                     /locus_tag="VYN80_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1540241.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            39926..40453
                     /locus_tag="VYN80_000265"
                     /pseudo
     CDS             39926..40453
                     /locus_tag="VYN80_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(40768..42459)
                     /locus_tag="VYN80_000266"
     CDS             complement(40768..42459)
                     /locus_tag="VYN80_000266"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1540242.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            42595..43041
                     /gene="argR"
                     /locus_tag="VYN80_000267"
     CDS             42595..43041
                     /gene="argR"
                     /locus_tag="VYN80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1540243.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            43092..45626
                     /gene="mutS"
                     /locus_tag="VYN80_000268"
     CDS             43092..45626
                     /gene="mutS"
                     /locus_tag="VYN80_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1540244.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            45773..47467
                     /gene="patA"
                     /locus_tag="VYN80_000269"
     CDS             45773..47467
                     /gene="patA"
                     /locus_tag="VYN80_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1540245.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(47797..48060)
                     /locus_tag="VYN80_000270"
                     /pseudo
     CDS             complement(47797..48060)
                     /locus_tag="VYN80_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            48258..50024
                     /gene="patB"
                     /locus_tag="VYN80_000271"
     CDS             48258..50024
                     /gene="patB"
                     /locus_tag="VYN80_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1540246.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            50163..50852
                     /locus_tag="VYN80_000272"
     CDS             50163..50852
                     /locus_tag="VYN80_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1540247.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            50856..51035
                     /locus_tag="VYN80_000273"
     CDS             50856..51035
                     /locus_tag="VYN80_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540248.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            51022..52371
                     /locus_tag="VYN80_000274"
     CDS             51022..52371
                     /locus_tag="VYN80_000274"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1540249.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            52495..53955
                     /locus_tag="VYN80_000275"
     CDS             52495..53955
                     /locus_tag="VYN80_000275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1540250.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            53969..54400
                     /locus_tag="VYN80_000276"
     CDS             53969..54400
                     /locus_tag="VYN80_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1540251.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT        15989 a        10326 c        11842 g        16321 t
ORIGIN      
        1 aaccatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtattaa
       61 tgatagataa agtggtcagg aacttactcc tgaccttttt cttttgcaaa atgacaaaaa
      121 tcataaactt tttgacaaat atacttgtca aaaagaaaaa gatatgttac aatgaattca
      181 agttaagaaa taggaagcag aaaggagtta taatgtgggt actaggattt atactattta
      241 tgattttctt ttattctaat aattctaaaa aaatcaagaa actagagaat aaaatcaaaa
      301 aacttgagcg aaaagagaaa ggaaacgcag aaatgtcgag attattacaa gaaatgattg
      361 gaaaggaacc aattataacg ggagtgtata ttgggccaga taactgggaa gttgtggatg
      421 ttgatgagga atgggtcaaa ctacgtagtg ttgataatac gggaaaagaa aaattcaagt
      481 tgcaacgtat tgaagatatc caaaccgttg aatttgacgg agagtaggtg tgtaacgtgc
      541 aactaaaaaa tcgtctaaaa gagcttcgag ctcgcgatgg tctcaatcaa accgacctag
      601 ccaaactggc aggggtttcc agacagacca ttagcctact agaacgggat gagtacaccc
      661 catccattat cattgccctg aaaatatctc aaattttcaa cgaaacagtc gaatcggtat
      721 ttcgtttgga ggaggacgag tgatgaacaa gtataaagtg atttattatg tagtggtcat
      781 agctttatta gtcagcgtat ttctactgat tgggatggac ctaagctggt ttaatcccta
      841 tcaaggcgac caatttgttt ggctctactt tgctctcatc ccagtaattg aatggattga
      901 aaagaaatcc aaaaatctag caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga
      961 ttgttatttt tgatgtctat tgtcttgggg gggttcttgg gcatgtttgt agggatgttt
     1021 aaggcacgtg tcgaatccca cgaaattatt ttagatgtaa aagccttgat gccatggata
     1081 tcagctattt gtttactgat aggtttcatt agtatgtttt tgactttcaa tttcttaaag
     1141 aaaagcagaa aatttcattc cttgtatcaa gaggaaatgg atgacgatct gaatgaaacc
     1201 tattatgtgc aaatgtatcg gaatcttgag tttggaacca ttgcttttaa tattacaggt
     1261 gtagcgattc cattggctat ttttatttca ttaagtgagg tgattatatt gcatacaaac
     1321 cctcaaacat ttttcctttc tttcttactc tttgtggtat tcttagtcgc tcaaaaatct
     1381 ctttttaaaa ccattgcgat tgttcgtcag tttgatttgg aatttttcgc tacaccaaag
     1441 gatgtcttga actatataaa ttcttatgat gaaggggagc gtcaggctaa tttggaacag
     1501 agttttcgaa ttttattcca attacaccaa tatgtcttac cagccttata tatttttctt
     1561 attatcattt ctttcttgac aggagagatt cagttactag ctttcttgct tgtaggagcc
     1621 atccatgttt atatcaatgt gatgcagtta cctatggtaa aacgttattt caaataaagg
     1681 agttatctat gaaattactt aaaaaccttg gctggattct tctagccctt ctatcctttt
     1741 tatttatcta tggctttatt caggggctcg cgactgcgtc gcttgcttta ggcgcttcac
     1801 cctatgctgt tactctactc tatgtggcct tggctggagt atatgtgtac ggcatttaca
     1861 aatggtatca gaaggctcct gttcatattg agaagagcgg ctttaaccga tttatttggc
     1921 ttcctgcctt ggtttggttc ttatctctgg tcgttcaatt cttcttgcca gatgatcctt
     1981 cagtaaatca gcaaatagcg acagacttga ccttgtctca accacttttc tcattctttg
     2041 ctgtggttat ttttgctcct ttgacggaag agattgtttt tagagggatg ttagcacgct
     2101 atctctttcc taagcaggac aatagtaaac gaaccctgat ttttcttctg gtatctagtc
     2161 ttctatttgc cttgattcat tttccaggtg atgtgcaaca attttttgtc tattttagcc
     2221 ttggttttag tttgggcttg gcttacatta gcagaaaagg tctggtctac agtatttctc
     2281 ttcacgcttt gaataattta gtcggctttt tgatgattct catgctataa tagagtcagg
     2341 aggtcacatg aaacgagtaa ttttattagc agtgattcag gcagtcgttc tatttttcat
     2401 cattggagcg ctagcttatg ccttcaaagg cgatttcttc tataactatc tagcagttgt
     2461 ctttgctcct attgcaggtg tactgcgttt tgggacggct tacataacgg aaattgtctt
     2521 gcctcgaaag gcagccgaaa tcgctgaaaa gcgtaaagca ggcaaaaatt caaaataaaa
     2581 gagtccggtg aagttcatcg gatttttgta ttatactcaa tgaaaatcaa agagtaaact
     2641 aggaagctag ccgcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa
     2701 gtcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacc
     2761 tacggtaagg cgacgctgac gtggtttgaa gagattttcg aagagtatta gcgctaattt
     2821 ttagggcttt cacttgactt ttaaagactg gcaaactttt ctgatgattt tcatgaagag
     2881 cctgcgcttt tatggtaaaa tagtaacaga ataaaagagg agagaaataa tgaaacgtag
     2941 tatgtatgct ggtcgtgttc gtgaggaaca catcggacaa gaaataacct tgaaaggatg
     3001 ggttggccgt cgtcgtgacc ttggtggttt gatctttatc gatcttcgtg accgtgaagg
     3061 aatcatgcag ttggttatca accctgaaaa agtatctgca gaggttatgg caacagctga
     3121 aagccttcgt agcgaatttg ttattgaggt gactggtcag gtcgctgcgc gtgagcaagc
     3181 caatgataag ttgccaactg gtgcggttga gttaaacgtg acagctctta ttgtgcttaa
     3241 cacagctaag acaacaccat ttgagattaa ggatggcatt gaggcaaatg acgatacacg
     3301 tttgcgttac cgttaccttg accttcgtcg tccagaaatg ttggaaaatc ttaaacttcg
     3361 tgccaaggtg acccactcta tccgcaacta tttggatgag ttggagttta tcgacgtgga
     3421 gacaccattc ctttctaagt caacgcctga aggggcgcgt gattatttag tgccgtctcg
     3481 tgttaataag gggcattttt acgctcttcc tcaaagtcca caaatcacga aacagctctt
     3541 gatgaatgct ggttttgacc gttactacca aatcgttaaa tgtttccgtg acgaggactt
     3601 gcgtggagac cgccagcctg aatttactca ggtcgacttg gaaacgtcct tccttactga
     3661 gaaagaaatc caagatatta cagaaggctt gatcgcgcgc gtgatgaagg aaacaaaagg
     3721 catcgaagta acgctaccgt tccctcgtat gaaatacgat gatgctatgg ctctttacgg
     3781 ttctgacaag ccagatactc gttttgacat gttgcttcag gacttgacag aagtggtcaa
     3841 aggtgtagac tttaaagtct tttcagaagc acctgctgta aaagcgattg tggtcaaagg
     3901 agctgcggac aactattcac gtaaagacat cgacaagatg acggaagtag ccaaacagta
     3961 tggtgccaaa ggtcttgctt gggtcaaggt ggttgatgga gaattaaacg gaccagttgc
     4021 caagttcttg actggtatcc aagaagaatt gacaacagcg cttgctcttg aagataagga
     4081 cttggttctc tttgtggcgg atacgcttga agtggctaat gcaacactgg gtgcccttcg
     4141 tggacgtatt gctaaagagc ttggcttgat tgataatgat aagttcaact tcctttgggt
     4201 ggttgactgg ccgatgtttg aatggtctga agaagaaggc cgctacatga gcgcccacca
     4261 tccattcaca cttccacagg aagagactgc tcacgaatta gaaggtgatt tggctaaggt
     4321 tcgtgccatt gcttacgata tcgtcttgaa cggttatgag cttggtggtg gtagccttcg
     4381 tatcaaccaa aaagaccttc aagaacgcat gttcaaggct cttggtttct caactgaaga
     4441 agccaaagac cagtttggtt tccttcttga agccatggac tatggtttcc caccacacgg
     4501 tggtttggct atcgggcttg accgttttgt catgttgctt gctggagaag aaaatatccg
     4561 tgaagtcatt gcctttccta agaacaacaa ggcaactgac ccaatgacac aagctccatc
     4621 aacagtcgct ctcaaacaac tagaggaact cagcttacaa gtagaagaag atgaaacaaa
     4681 caaaacgaat taagcggtgg cgctattatc tgcgccgctt tgcttatcag ataaaaattt
     4741 tacgtgtctt acaaagtatc tctcgagaaa agtatgatga gaagatttcg gcctctctgg
     4801 tctatggttt tttatcagca gtagcagtta atttcttttt ccaaccaggg catgtgtatt
     4861 cgagtggtgc aacaggtctg gcacagatta tctctgcctt gagtaatcac tggtttggtt
     4921 ttcatattcc gatttcgcta agcttctacg ccattaactt ccctttgatg gtcttagctt
     4981 ggtatcagat tggccataag ttcaccgtct ttacctttat cacggtatct atgagttcct
     5041 tctttatcca gtttgtccct gtggcaacct tgacggagga tcccattatc aattcccttt
     5101 ttgggggtgt tgttatgggt ttggggattg gttttgctct tcgaaacaat atctccagtg
     5161 gtgggacgga tatcgtcagc ctgactattc gcaagaaaac gggtaagaat gtcggtagta
     5221 tttctttctt ggtaaatgga actatcatgc tgatagcagg tttgaccttt ggttggaaat
     5281 acgctcttta ttctatgatt accatctttg tctctagccg tgtgacagac gcagtcttta
     5341 ctaagcaaaa gcgtatgcag gccatgattg tgacaaatca tccagagaag gtaattgaaa
     5401 aaatccataa aaaattgcac cgcggagcaa ccatgatcca cgatgcagaa ggaacctata
     5461 atcacgagag aaaggcagtt ttaatcactg tcattacacg tgcagagttt aatgaattta
     5521 aacagattat gacacaagtg gatccaagct cctttgtctc tgtctcggaa aacgttcata
     5581 ttctaggaag atttgttgag atagataatt agtgattaat actcttcgaa aatctcttca
     5641 aaccacgtca gcttcacctt gccgtatata tgttactgac ttcgtcagtt ctatctgcaa
     5701 cctcaaaacc atgttttgag ctgacttcgt cagttctatc tgcaacctca aaaccatgtt
     5761 ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag ctgacttcgt
     5821 cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt
     5881 gcactttgat tttcattgag tataaaaaga tcttgggaaa agactcaatt tcagtagaaa
     5941 aggaagtaaa tcttcccaca ataaaacgca tagtatcaag ttttttcaat acctgatagt
     6001 atgcgttttt gcttttaacg actctttgcc cagtctctta tttttcgata attttgtggg
     6061 cgatcaattg acggtaacaa atttgtttat tgtttttatt atcattaata atctggagaa
     6121 tagtttcaag ggaaacacga ccaagctcta aactattgat atcgacatag gctgccaagt
     6181 tgagcttggg attaaccgag tcaaagctga gaacagggac atccagctgg tgtttggcaa
     6241 tatagttaca aacaccttca gctaggaggc tatcggttgt gatgatagca tcaatttgtg
     6301 gatcgtgctt gaataatcgc ttgctaaatt tatagccctt ttcttccaga aactcgtcgg
     6361 caaagtagat gcgattgttg tcagtggtaa gtttgtaatg tttaagcgcc tgttcatagc
     6421 ccgttaaacg gtctttggtc acgaagagct ttttacttcc tccgataaag gcaatgcgtt
     6481 tgcagccttt tttgatgaaa tattcagtcg catcaaaacc agcttgaaca ttgtcgttgt
     6541 cgacaagtgg gatgaaagga gatagagatt tacctaagat aaggaagggg aactgttctt
     6601 ctgcgacgag ttttacgaga gggtcttctt cttgggcata gagaaagatt agcccatcta
     6661 cacgcttgcc gtagaccatt tgtgaaatag cgttgagacg ctccttctca tctttccctg
     6721 ttgctatttg aatggcatag tggttttcag atgcgacttg agagatgcca cgtagaaccg
     6781 atggaaagaa aggattctgg tagaaggcgt ctgagtcatc aggaagaact aatccgataa
     6841 cctgagtata gctgcttacc aagctacgag cgttaaggtt tgggtggtag ttgagttcct
     6901 tcatagcttt gcgaacgcgt ttttttgttt cgtcgctaat ggttgattta ttttgaataa
     6961 cacgggttac ggttgaaggc gaaacaccag cagccttggc cacgtcttta atcgtaacgg
     7021 gcataaaaat ctcctattta tggtaatttg gatcacggaa atgtgtttta taaaagatag
     7081 tgaccagata cagaacaaaa agaatatttt gtacagtaat cagggttgtc atattttggc
     7141 caaataatcc caaaataagt gtaatcagag tcggcaatcc taaacagttc aagataaagt
     7201 gatagcactc tttaaaggtt ctaaatgaaa agaggcgtga tttcttagtg atataaagga
     7261 gaagactagc tcctagagag acgataaaga aattcaaacc aaagaggaag ctcgcaccga
     7321 gaactaggaa gaggctgata tagacacgat tttgttggta ccagtcttta gaaattgctt
     7381 gggtcaagct gtctttgctt ttgaaactct cagtctgaat ggctcggtaa gagatgcggg
     7441 tcagttcctt gctttccttg ctgatgacta gttcatttgt atcgaaatgc agttgcaagt
     7501 ccttaggtaa ttccttgatt tgacctggac caatcacaac agaaggggct tgactagctg
     7561 ttccagtata agttaatgtg ccatcgacaa ttgtagcatg ttcagagaga tcctggacaa
     7621 cctcatttgt cagaggttca tagacattat cgataaaagt ttctagcgga taggtctcct
     7681 gggagctgtt ttggatagca atgggtacca tagacaagct gataaggaag atactggtaa
     7741 agagaagttg aaaccagttg agctcgaaac gtttggacag gggcttacga aacccccaaa
     7801 tacttgaaaa ataggaaaat ggatatggaa gcataggtat ctttctaaaa ggtgttttct
     7861 atatgagtat tattatatag agaaatgcgc tttatttcaa gtctaggggg taaattgctt
     7921 gctacaagga tattatttac agtgacaatc gctaatagtc aatgaaaatc aaagtgcaaa
     7981 ccaggaagct agctgcaggt tgctcaaagc acaactttga ggttggagat aaagctgacg
     8041 tggcttgaag agattttcga agagtataaa atgaaaaagg gtggcgggga taaattatcc
     8101 cttgtcgcca ccacttgtaa gtcctgaaac aaagttcttt tgtaggaaga agaagagaat
     8161 acagattgga agggcgatga ggatagcacc tgctgagaag taggcaatct tcaagttttt
     8221 cgcattgtta acgaaggttt ggagacctac ggcaacagta aagtattctt tctcacgaag
     8281 caagaaacta gagaggatgt agtccccgaa aggtcccatg aaggcccaga gagcttgtac
     8341 ggcaaccatt gggcgaacaa gtggtagaac aatttgccag aagcggcgga agtgtcctgc
     8401 accgtctagt tttgcagatt cgtctaaaga cattggcact gtatcgaagt agcctttcat
     8461 gagccaagca ttcatcggga taccaccacc aacgtagagg aagatgagga accagttgtg
     8521 gttaagggcg ttcaacataa gcgccataac gaagaaggct gtcaaagcgg ccatagttgg
     8581 caccatttgg atgatcaaga agaagaccaa actttgttta cgagccaaga agttgtaacg
     8641 gctgtaagca taaccagcaa gtacgatgat acttgtttga acagccatgg taattaaggc
     8701 gataatcaaa gtgttgaggt accaagtacc gtacaaggtt tcagtgaaga ggcctttaaa
     8761 gttatcaaaa ttgaggtcga tattagtatc tagtttaaag gctgagacgt tacctgcttt
     8821 aaaggctgac atgatggtaa tcaacagtgg atagataatt acaattgata gaccaatcag
     8881 gtaaaggtaa gtaaggcttt gagtcagtct acgtttgagt ttaattgagt tattcatctt
     8941 agacgtcctc catatcaaat gcgtgtagtt tcttgaatgc gatcatagag attgagatga
     9001 caatgataga gataatcaag gtaacagctg ccgccattga gtattgagga gatgtacctg
     9061 ttgtcaaacg gtagatccat gagatcaaga tatcggttga accagctcca cctccgacac
     9121 taccaggtcc tccaccattg aagaggtaca tgatagagaa gttgttaaag ttgaaggtgt
     9181 attggctaat caaagtaggt gccgcaacag ccaaaatcat tgggaaagtg atgttgcgga
     9241 atttttgcca agcgttggca ccgtcaatat aagctgcttc gtaaaggtcg ttaggaatag
     9301 attgcaagat acccaaagtc aaaacgtaga tgtatgggaa tccgagccaa ccttgcatca
     9361 taatcaaggc aatcttagtc caagttgggt ctgttttcca aggaataaga gctccatcaa
     9421 ggaaaggaag gaatttagcc aagattggca atacttgagt gttgatagca ccgacactat
     9481 cgttaaacat gtttgagaat gtcaagatag tgatgaaggc tgggacagcc caaggaagaa
     9541 ggaaaataac accaaagata cgttttcctt tgataaatgg ttggttggca atgatagctg
     9601 tgaagatacc aattacgatt tgtaaagtag aagctgccaa agcccaaatg atagtccaag
     9661 aaagaacaga accaaaggca gaacggaagg tactcaagct ccaaatgttt gtaaagttgg
     9721 tcaaaccaac ccagtccaac aacttgtttg gtggcaagtg ttggaagtcg tagttggtaa
     9781 aggcgatcat caaggttacg ataactggga agataatcgc gaaggtcatg gcaacataag
     9841 atggaatgat caagaggtaa gggaagccat tttcataaat ccctttgatc atgtctttga
     9901 gtgtgcgtgg aactggaatt ccattgttaa tgcgttttgc aatcgtatgt gcatctttga
     9961 tatttgagaa ataaaagagt acataaacga ttacaaagat tagatggaag gcaccacgaa
    10021 tcagcataaa gagggaatta tcacgacctg gtttgtcacc aagagtgatg aggttgctca
    10081 attcaggggt tgcaagtgct aggaagtaaa ggacaaatac gatggttaca ccaaggaaga
    10141 taaaaccttt ggctttttgt ttattgtaaa tctgtcctaa cccaggaatg atagacagca
    10201 gggctgcttt actaggttgt tgcttttcca tattaactcc tttcatagga tactactttt
    10261 ttataagaaa gctaaattat ttgtgtctct attgataaat tcaaaggggg atttgatttc
    10321 cacccccctt gaacaaattt tttattcacc aaatttttgt ttgattgttt ctttgatcaa
    10381 tgttacagca tcgttagcag ctgttttagc atctttttga ccacttacag catcaaagag
    10441 catatttttc gctggatccc aaactgcaga catttgagag atgtttggca gtggttgagt
    10501 gttcttgaac tgtttgataa cagctgttgt caactcatcg tttttacctt cagcgtatga
    10561 acgagcctca gtattagctg ggatttcgtt agtcttatca tataatactt tttgttgttc
    10621 agttgcaaca aggaagtcta caaatttttg agaagcttca aggttcttaa cggcttgagg
    10681 aatgacccaa gctttaccac caccgaatgc agcatattct tttccatttg gaagagttgg
    10741 gatagttgca actccgtagt ttactttagc atctttaaag gcttgagctt tccaaggtcc
    10801 gtcgatgata gcagctgttt taccttcttg gaattgagtt tggattaagt ttccagcacc
    10861 ttctgtatct tgcatacctt taggccattt ttcgtaccaa gatttagcgt agttgatacc
    10921 tgcgatagaa ccgtcgtttg caagaccgat gtctttagcg tctttaccgt tttggccaaa
    10981 gacgtaagca ccgttaccgg caagaagtcc atatgtatag tagaagtttg tccagtcagc
    11041 taggaaggca gtagttttac catcttcacc agcgaatgcg tatttgctat ctttagcaag
    11101 gttttccaag tcagcaaatg tttttggagc atctttcacc aagtctttgt tgtagtacat
    11161 aacaagtgac tcgataacgg caggagcacc gtaaacttta ccattagcag ctgttacaag
    11221 agatttagtt gtgtcgtctg ttttagcacc atcgctcaat ttcacttctg aaagttgtcc
    11281 gtcagaacca aggctaccta cacggtcgta tggagccatc ataacatcag ggacattacc
    11341 agattggttg tcaagagaaa gtttatcaag acctcctaga gcatcaccag ttttaagagt
    11401 gacttttact ccagcttctt tttcataagc tttagcaacc tcttcaatat agctcttata
    11461 tccctcgtct acatatacag tgagttcttt gacttcagat gaaccagaat cagcaggctt
    11521 atcagcagtt ttgcttccgc aagctaccaa aagcaagcta gcaagtgtag cagttccaag
    11581 caccgcagtg ctcttcataa atttagatga catagtgtat tcctcccaaa gaatagcaag
    11641 ttttattgat aaggaaacgc aaacgttttc ctttatgagc ttagtatagc acaaatagaa
    11701 aacggttgca agtatttttt gtaaaaattt taaaaaattt ttaagcttga tttgatagca
    11761 taattttgca ttttaataga agaaaagtat gaaatagata agaatacagt atttttaata
    11821 aaaatatatg gaatctgtct aaagagggaa atcaggagga gctacctccc tgtttctaat
    11881 ccctatctat tttctataga cgtttgtgct ttgttgcata taaattcaag ctccctaagg
    11941 aggttttgta ctttgtagac tgtctcgtga gagtcaaact ttaggattag atagttttaa
    12001 gttatctttg ctaaaatgag ttagtctcct taaaaataat caaaaaagtt ttttaaaaac
    12061 gcttgcaatt atgcttgaaa aggagtatac ttataagtaa cgcaaacgtt tgcgtctgca
    12121 aaatacgcaa cgttccatta ttttaacaca cgaggtgcta ttatgaaaaa acgtcaaagt
    12181 ggtgtgttga tgcacatctc ttctcttcca ggagcttacg gaatcggatc atttggtcaa
    12241 agtgcttacg acttcgttga tttcttggtc cgtacaaaac aacgttactg gcaaatcctt
    12301 ccattaggag caactagtta cggggattct ccttaccaat ctttctcagc cttcgcagga
    12361 aacactcatt ttatcgattt agatatcttg gtggagcaag gtttgttgga agcaagtgac
    12421 cttgaaggag ttgactttgg tagcgatgcg tctgaagttg actatgctaa aatctactat
    12481 gcacgtcgtc ctcttttaga aaaagcggtg aaacgtttct ttgaagtcgg agatgttaaa
    12541 gattttgaga aatttgctca agacaaccaa tcatggcttg agctctttgc tgagtatatg
    12601 gctatcaaag agtattttga caatcttgct tggactgaat ggccagatgc agatgctcgt
    12661 gctcgtaaag cttcagcact tgaaagctat cgtgagcaat tggcagacaa gttggtttac
    12721 caccgtgtga ctcaatactt cttcttccaa caatggttga aattgaaagc ttacgctaac
    12781 gacaaccaca tcgaaatcgt tggggacatg ccaatctacg tagcggaaga ttcaagtgat
    12841 atgtgggcaa atccacatct cttcaaaaca gatgtcaatg gtaaggctac ttgtatcgca
    12901 ggatgcccac cagatgagtt ttctgtaact ggtcagcttt ggggtaatcc aatctatgac
    12961 tgggaagcaa tggacaaaga cggctacaaa tggtggattg aacgcttgcg tgaaagcttc
    13021 aaaatctacg atatcgttcg tatcgaccac ttccgtggct tcgaatctta ctgggaaatc
    13081 cctgctggtt ccgatacagc agcacctggt gagtgggtga aaggtccagg ctacaagctt
    13141 tttgcagccg ttaaggaaga acttggtgag ctaaacatca tcgcagaaga ccttggcttc
    13201 atgacagatg aagtgatcga attgcgtgaa cgtactggct tcccaggaat gaagattctt
    13261 caatttgcct tcaacccaga agacgaaagc attgatagcc cacacttggc acctgctaac
    13321 tcagttatgt acacaggaac acacgataac aatacggttc ttggttggta ccgtaatgag
    13381 attgatgatg cgactcgtga gtacatggct cgttacacga accgtaaaga atacgaaaca
    13441 gtggtacacg ctatgcttcg tacagtattt tcatcagtta gctttatggc aattgcaact
    13501 atgcaagatt tactagaatt ggatgaggca gctcgtatga acttcccatc tacccttggt
    13561 ggaaactggt cttggcgtat gactgaagat caattgacac cagctgtcga ggaaggtttg
    13621 cttgacttga caacaattta tcgccgaatt aatgaaaatt tggtagattt aaagaaataa
    13681 gacaataatc aggagacaac taaacatgtt atcactacaa gaatttgtac aaaatcgtta
    13741 caataaaacc attgcagaat gtagcaatga agagctttac cttgctcttc ttaactacag
    13801 caagcttgca agcagccaaa aaccagtcaa cactggtaag aaaaaagttt actacatctc
    13861 agctgagttc ttgattggta aactcttgtc aaacaacttg attaaccttg gtctttacga
    13921 cgatgttaaa aaagaacttg cagctgcagg taaagacttg atcgaagttg aagaagttga
    13981 attggaacca tctcttggta atggtggttt gggacgtttg gctgcctgct ttatcgactc
    14041 aattgctact cttggtttga atggtgacgg tgttggtctt aactaccact ttggtctttt
    14101 ccaacaagtt cttaaaaaca accaacaaga aacaattcca aatgcatggt tgacagagca
    14161 aaactggttg gttcgctcaa gccgtagcta ccaagtacca tttgcagact ttactttgac
    14221 atcaactctt tacgatattg atgttactgg ttatgaaaca gcgactaaaa accgcttgcg
    14281 tttgtttgac ttggattcag ttgattcttc tattattaaa gatggtatca actttgacaa
    14341 gacagatatc gctcgcaact taactctctt cctttaccca gatgatagtg accgtcaagg
    14401 tgaattgctc cgtatcttcc aacaatactt catggtttca aacggtgcgc aattgatcat
    14461 cgacgaagca atcgaaaaag gaagcaactt gcatgacctt gctgactacg cagttgtcca
    14521 aatcaacgat actcacccat caatggtgat tcctgaattg attcgtcttt tgactgcacg
    14581 tggtatcgag cttgacgaag caatctcaat tgttcgtagc atgactgcct acactaacca
    14641 cacaatcctt gctgaggcgc ttgaaaaatg gcctcttgaa ttcttgcaag aagtggttcc
    14701 tcacttggta ccaatcatcg aagaattgga ccgtcgtgtg aaggcagagt acaaagatcc
    14761 agctgttcaa atcatcgatg agagcggacg tgttcacatg gctcacatgg atatccacta
    14821 cggatacagt gttaacgggg ttgcagcact tcatactgaa atcttgaaaa attctgagtt
    14881 gaaagccttc tacgaccttt acccagaaaa gttcaacaac aaaacaaacg gtatcacttt
    14941 ccgtcgttgg cttatgcatg ctaacccaag attgtctcac tacttggatg agattcttgg
    15001 agatggttgg caccatgaag cagatgagct tgaaaaactt ttgtcttatg aagacaaagc
    15061 agctgtcaaa gaaaaattgg aaagcatcaa ggctcacaac aaacgtaaat tggctcgtca
    15121 cttgaaagaa caccaaggtg tggaaatcaa tccaaattct atctttgata tccaaatcaa
    15181 acgtcttcac gagtacaaac gccaacaaat gaacgctttg tacgtgatcc acaaatacct
    15241 tgacatcaaa gctggtaaca tccctgctcg tccaatcaca atcttctttg gtggtaaagc
    15301 agctccagcc tacacaatcg ctcaagacat tatccattta atcctttgca tgtcagaagt
    15361 tattgctaac gatccagcag tagctccaca cttgcaagta gttatggttg aaaactacaa
    15421 cgttactgca gcaagtttcc ttatcccagc atgtgatatc tcagaacaaa tctcacttgc
    15481 ttctaaagaa gcttcaggta ctggtaacat gaaattcatg ttgaacggag ctttgacact
    15541 tggtactatg gacggtgcta acgtggaaat cgctgagttg gttggagaag aaaacatcta
    15601 catcttcggt gaagattcag aaactgttat cgacctttac gcaaaagcag cttacaaatc
    15661 aagcgaattc tacgctcgtg aagctatcaa accattggtt gacttcatcg ttagtgatgc
    15721 agttcttgca gctggaaaca aagagcgctt ggaacgtctt tacaatgaat tgatcaacaa
    15781 agactggttc atgactcttc ttgacttgga agactacatc aaagtcaaag agcaaatgct
    15841 tgctgactac gaagaccgtg acgcatggtt ggataaagtc atcgttaaca tttctaaagc
    15901 aggattcttc tcatctgacc gtacaatcgc tcagtataac gaagacatct ggcacttgaa
    15961 ctaatactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc tcaaagcggt
    16021 gctttgagca acctgcggct agcttcctag tttactcttt ggttttcatt gagtataaga
    16081 tacaaattta tactaataca ttttgtaaaa aagcgagttt cgattgaaat tcgctttttt
    16141 aaatgctgta gatttgggtc aatcttgtct aaaaataggg aaatcctaga tacagtgaag
    16201 actttaaatg ctggttttta ctgtcctcag ccttatattt tttcgtagtt gattacctca
    16261 cacctcttgt attcgcttac ataaagtatt ataatataat tgtaggaaag aaggtgtttt
    16321 tatgatatac acacttaaat tggtgttgtt tattaccttt cttgtaataa gcttgttacc
    16381 tgataagatt tttggaaaaa ataaaaaaat ttggaaaata gtttttgcaa tattgacggc
    16441 agtggcagca ttgtcattta tgtactaagt tattttaaga atgtagggaa ataaacccta
    16501 cattcttttt agttttttct gttttctaaa ttctatttat ccaagcgctt caacatttct
    16561 tgcttcttcg cttcaagttc tgcacgcttt tcttcgattt cggcatgttt tttctcgagt
    16621 tcagaacaac ttgcaccatt gctaaattct tttcgccatc aggagatagg gtgagtcgac
    16681 atgtctatta ctcacccaaa gcagtcctac aaagcaggaa ttttctgtta cttttttgga
    16741 aatagtaacg tttatacagc tttgacactt cgtatcaaag cgccaaacac actccgaggg
    16801 atttacagaa agcagaaaag gaatgatctg gtataagatc attccttttc tctctttttc
    16861 tttaagtaat tatatacaat gtacgacgaa gtcgtcattg caatgctgat ccaccaccta
    16921 aagggaactt taaacaacat tgataagata aagaatataa acaacgaaaa tacgttatac
    16981 ccaattaatt ttattgtata tctcatgatt aaaagttaat ccttccgttg ttaggaatgg
    17041 catcattttt atcccataat tgtgctaaat aagtccccgg tgataataaa ttcatagcgg
    17101 attctaaagc aacatcattt acaaaccaac tacctagata tctagaaatt gctgaacgaa
    17161 tagcactttt tgctgcatgt tttcctttta ctttaattag atttgcaagg cctgcagtag
    17221 ttcctcctaa tgctaaagct attgtagtat ctaatagagc acccatttga ttaactgtaa
    17281 taccttgcca agctgctcta aatggagagt atgtaggtgg gattgtataa tcgccttgta
    17341 attgtcggtt aattacttct ttgatccatt gttgtgagac gtctggatga aaagattgga
    17401 tttcgtttgc aagtgtattg atttgttctt ctgttagaga agtgacaggt tgaagttcca
    17461 tatttgtttc aatttgcgat acttgttcag aagcgtatac agctgaaaca cttggaatcg
    17521 ctgatacaat taacacaatt gacgtcaaaa aaaccgaaat aaatttcatt aatttgttca
    17581 tgagcttttc tcctttttat ttgtatctgc ttacatttta ttatatactg ttattatagt
    17641 caagaaaaat atgctattat gttaaaaaaa tatttttcaa aatataaatg gacggattta
    17701 ttttggattt tatttgttat tttgacctgc ctctatattg gtaaccatga tttgtttact
    17761 ctcaatcatc aagaattctc ttttcgtggt agcttttggg gtttggtact ggccttatat
    17821 cacttattgt tcattgataa gtttgtaatt tcgaatcgaa aataaagatt agaactatga
    17881 ttgactggct acttttaata ttgattttgg aggaagattt tgtctctatt atttattatt
    17941 ttaaatttat ctattttgta caatatctat tctttgagac gggagaaatc gaaatacttg
    18001 atttatacgg cctatatcat atttggggta aatgtagtat atggtattca atggttatta
    18061 agagaactga tttcaactat ttccccttaa tgtgtatgga aactataaca aaacgcataa
    18121 gattaaggtt tgtgtacctg ttcttatgcg tttttgtaat attgaagatt ttcggtaagt
    18181 tttatacctt tgggaagtct agtttagaat gctttcccaa ccagaatctc tgcttcgatg
    18241 gtaatctctg tcagttggct ccagtcaatc ttgctctggt caacatgaaa gaggagagga
    18301 gtcatactga gtagggcttg gagttgttct gctgtgatag tcttagtcag agaggcagtt
    18361 tgactagata ggatggtaaa gtgttcctgg aaatgctcct tgatatcttg gttagaatac
    18421 tccttgtttg tcagctggtc ctgtactctt tgacggattt ctttgaggtg attttcagtt
    18481 gggataacct ttatcaagat accgtctttg gataaaacgc gacgaaattc tccatagttg
    18541 gcaggtgaga agatatcaag cagaatatcc atgttagcgt cttttatagg aagtcgtgcc
    18601 aagtcgccaa cgaaccaatt gactgcccag ttgggttcac ttttagcagc gatttggact
    18661 gaatctttgg agatgtcaaa ggcatagaaa gttttttcag agtgactttc ttgtagtttg
    18721 cgagaataga atccttcacc acaaccgata tccaaaattg ttgtggtagt ttttgagctt
    18781 gcaagcaagt cagatacagc atctaagata gcttggtaaa agccggcttc taggatttgt
    18841 tgacggtttt gaaaattttc cttgtcatag ttagcagatt gcttgatttg aggtgctaga
    18901 ttgacatagc caaatttcgc caagtcaaaa gaatgacggt tgcagcactt gaaattagtc
    18961 tctaacagag tcagattttc ttgacagata ggacaggcaa aggcagtcgc agaagcaaaa
    19021 cgctgaagtt tgggcttgag atttgtattc atagtttaag tgtatcaaaa gaggcaaatg
    19081 aaagcaactt taggaataag tagatgggag attcagtaga aataaaacta attctccaat
    19141 ttatagaatg aaattgcttt tatatctaaa ttgctatata ataatgataa ggaggaaata
    19201 agtatgttaa aagaagtatt aaccgttgca aaagttgcga aaaaatcttc attatttttg
    19261 ggtggtgtcg catttggtac ccttggtttg aaaatcttag caagtaagga agctaaaaaa
    19321 ggttattcta aagctttggc taaggcttac aagttgaaag acgagctaga tgcatctgtt
    19381 tctgttgtga agcaacatgg agacgatgtc ttgcaagatg ccaaatattt gtacgagcaa
    19441 gagaaaaaag aagagcaatt agatagcctt ataggtgaat aatatgtctt ttaaagtgct
    19501 acatagagga taccaacata tccgactatc atcttctttt tcacttacct tggatattca
    19561 agactatctt cgttccttgg cgagagatga aaaggggatt gagtctatcc agttttacat
    19621 ggatcaacag cactttactc tacgcataaa agaaggcttt tctgtattag ataatgcaga
    19681 agccttttta aaaagaattg ataaagggaa agtttctgag ttgatgactc ttcccattcg
    19741 tagagaagag agtgcttatt ctattgtttc aggtgcagcg gttaagcgtg tactttttcg
    19801 tagttttgtg ccgtatccta ttcgctatat atggacttgt tatcaggctt tgggttatat
    19861 tagagaagcc tatcaaacac tagcgcgtaa ggaactaacg atggaagtct tggactgttc
    19921 ggctatttta ttgtctttgt ttatgaacca atccaagaca gctagcaata tcatgtttat
    19981 gcttgatttg gggaatcatt tagatcagtg gtccttgaaa aaaactgcaa cagatttaga
    20041 acagagtctt cttgcaaaag agagcgatgt attcctagta cagggcgata cggttgttag
    20101 tatcaagagt tccgatgttc aaataggaga tgtcttgatc ttatctcaag gaaatgaaat
    20161 tctgtttgat ggacaagtag tttcaggttt aggtatggtc aacgaaagtt ccttgacagg
    20221 agagagtttt ccagttgaaa aaagagagtc tgatttggtt tgtgcaaata cagtattaga
    20281 aactggagag ttacgcattc gtgtaacaga taatcagatg aacagccgta ttttacagct
    20341 gattaagttg atgaagaaat ctgaagaaaa caagaaaacg aaacaacgct atttcatcaa
    20401 gatggcggat aaagtcgtta aatataattt cttgggggct gggctgactt acctattgac
    20461 aggttctttt tctaaggcta tttctttcct attggtcgat ttctcctgcg ctttgaaaat
    20521 ctctactcct gtagcttatt tgacagttat caaggaaggg ttgaaccgtg aaatggtgat
    20581 taaggatgga gatgttctgg agaaatatct ggaagttgat actttcttgt ttgataagac
    20641 aggaacaatc acaactagtt atcctatagt tgaaaaggtg ttaccttttg gagactatag
    20701 tgaggaagat attctcagaa tcagtgcctg tcttgaggaa cacatttatc atcctattgc
    20761 taatgccatc gtcaagcaag ctgagataga gggaattgaa catgaggaaa tgcatgggaa
    20821 actccaatat atcgcaagca aggggatcaa atctcatata gatggccaac cagttcttat
    20881 tgggaattat gtcttgatgc aggatgagca gattcatatc agttcagaac aaaatgcttt
    20941 aattgaagag tacaagagtc actacaatct cttattcttg gcttatcaaa atgaattgat
    21001 tggaatgttc tgcattcata ctcctttgag aaaagaagca aaaacagcct tggataaact
    21061 taaggcacag gggaaaaaat tgattctggc aacaggagac accttgatta ggacagagga
    21121 attagtcaaa gatttgccct ttgatcaggt ctatacagac ttgaaacctg atgggaaatt
    21181 tgagttagta gagaaactgc agaaagcagg tcacactatt ttgatggttg gagatggatt
    21241 gaatgactca gcggctttaa ctctatcaga tatcggtgtg gtgatgaatg agagtgcaga
    21301 tatttctaag cagatgagtg atatcttatt gttagataat cgtttggatt tcttccaaga
    21361 gttggattcg ttatcatcat ctttgcaaac actcatcaag aaaaatattc aagataccgt
    21421 tgtcgtaaat agtagtttga ttggctttgg cttatttaat tggctcagtc cttcaaatct
    21481 ctctatctta cataatctaa caaccttgcg cattgtcctg cgtagcctgt ctattaaaaa
    21541 tagatagatg agagttatta acagcaaaaa ggagttacct ttctcgaggt taactccttt
    21601 gtttatagat aaatgttgaa tagtttagta agtcaataca aagtattttt tcttcccacg
    21661 acggataaca gtcagttcat tttctaactt atcagcgtca ctcaagacat agtcaaggtc
    21721 ttggatgcgg tcgccgttta cgtagatggc tccgttttgg acgtcttcac gggcttggcg
    21781 ttttgagtta actataccag atgagacgag cagttccacg atattgttgt gttcgtctgc
    21841 ctgtacttgg tagtttggca caccacgaag tccttgtttg agctctttga cagaaaggtt
    21901 tttgatgttt cctgcaaaga gttgctcagt gatgttaagt gcttctttgt aggattcttc
    21961 tccgtgaaca agtgtaacaa cttcacgagc caagactttt tgagccaagc gttcgtgtgg
    22021 cgctgcttca aattgtttgc gaatatcttc aatctcatca agtgacaaga aagtaaagat
    22081 tttcaagaag cgaacagcgt cagcgtccat cacgttcatc cagaattggt acatttcgta
    22141 tggagaagtc ttttcgggat tgagccagac ggcatttcct tctgatttac caaatttctt
    22201 accagttgca tctgtgatta gtggaacagt gataacgtga ccagtcttgt ccgccttacg
    22261 acgaagcaat tcggtaccag ctgtcatatt tccccactgg tcagaaccac cgatttgaag
    22321 agtgacatta tggtcttggt taaggacgaa gaagtcatac ccttgcatga tttggtaagc
    22381 gaactcagtg taagaaattc ctgtttcgat ccgtttttta acagattcct tactcatcat
    22441 gtagttgacc gtgaagtatt ttccaatatc acggaggaag tcaatgaagc tgatgctgcc
    22501 aaaccagtcg tagttgttga ccatgacagc cttgttttcg ccattttcaa agtcaagaaa
    22561 acgagaaagt tgtccttgga tagacttgac ccagccatct actgtgtctt ttgtttggag
    22621 actacgttca gcatctttga aggacggatc tccgatgaga cctgtagcac cgccaacgag
    22681 cgcataaggt ttgtgacctg ctagttgcaa gcgacgactt gtcaagattg cgacaaggtg
    22741 gcctaggtga aggctgtcag cagttggatc gtagccagta tagtaagaaa cttgaccttc
    22801 ttctagggct ttacgcaaag cttcttcatc agtcgtttga aatatcaaac cacgctcttt
    22861 tagctcatca aaaatgtgca tgtgtctttt ctccttttta aaattattgt ttctacctat
    22921 tgtatcacaa acttgggcaa tagcctagca gaatagggaa gaaagtggct attttattga
    22981 aaatttccct tttgtggtat aatagataga gtgaggaaat ccatgcaaaa tcaattaaat
    23041 gaattaaaac gaaaaatgct ggaatttttc cagcaaaaac aaaaaaataa aaaatcagct
    23101 agacctggca agaaaggttc aagtaccaaa aaatctaaaa ccttagataa gtcagccatt
    23161 ttcccagcta ttttactgag tataaaagcc ttatttaact tactctttgt actcggtttt
    23221 ctaggaggaa tgttgggagc tgggattgct ttggggtacg gagtggcctt atttgacaag
    23281 gttcgggtgc ctcagacaga agaattggtg aatcaggtca aggacatctc ttctatttca
    23341 gagattacct attcggacgg gacggtgatt gcttccatag agagtgattt gttgcgcact
    23401 tctatctcat ctgagcaaat ttcggaaaat ctgaagaagg ctatcattgc gacagaagat
    23461 gaacacttta aagaacataa gggtgtagta cccaaggcgg tgattcgtgc gaccttgggg
    23521 aaatttgtag gtttggcttc ctctagtggg ggttcaacct tgacccagca actaattaaa
    23581 cagcaggtgg ttggggatgc gccgaccttg gctcgtaagg cggcagagat tgtggatgct
    23641 cttgccttgg aacgcgccat gaataaagat gagattttaa cgacctatct caatgtggct
    23701 ccctttggcc gaaataataa gggacagaat attgcagggg ctcggcaagc agctgaggga
    23761 attttcggtg tagatgccag tcagttgact gttcctcaag cagcattttt agcaggactt
    23821 ccacagagtc ccattactta ctctccttat gaaaatactg gggagttgaa gagtgatgaa
    23881 gacctagaaa ttggcttaag acgggctaag gcagttcttt acagtatgta tcgtacaggt
    23941 gcattaagca aagacgagta ttctcagtac aaggattatg accttaaaca ggacttttta
    24001 ccatcgggca cggttacagg aatttcacga gactatttat actttacaac tttggcagaa
    24061 gctcaagaac gtatgtatga ctatctagct cagagagaca atgtctccgc taaggagttg
    24121 aaaaatgagg caactcagaa gttttatcga gatttggcag ccaaggaaat tgaaaatggt
    24181 ggttataaga ttactactac catagatcag aaaattcatt ctgccatgca aagtgcggtt
    24241 gctgattatg gctatctttt agacgatgga acaggtcgtg tagaagtagg gaatgtcttg
    24301 atggacaacc aaacaggtgc tattctaggc tttgtaggtg gtcgtaatta tcaagaaaat
    24361 caaaataatc atgcttttga taccaaacgt tcgccagctt ctactaccaa gcccttgctg
    24421 gcctacggta ttgctattga ccagggcttg atgggaagtg aaacgattct atctaactat
    24481 ccaacaaact ttgctaatgg caatccgatt atgtatgcta atagcaaggg aacaggaatg
    24541 atgaccttgg gagaagctct gaactattca tggaatatcc ctgcttactg gacctatcgt
    24601 atgctccgtg aaaatggtgt tgatgtcaag ggttatatgg aaaagatggg ttacgagatt
    24661 cctgagtacg gtattgagag cttgccaatg ggtggtggta ttgaagtcac agttgcccag
    24721 cataccaatg gctatcagac cttagctaat aatggagttt atcatcagaa gcatgtgatt
    24781 tcaaagattg aagcagcaga tggtagagtg gtgtatgagt atcaggataa accggttcaa
    24841 gtctattcaa aagctactgc gacgattatg cagggattgc tacgagaagt tctatcctct
    24901 cgtgtgacaa caaccttcaa gtctaacctg acttctttaa atcctactct ggctaatgca
    24961 gattggattg ggaagactgg tacaaccaac caagacgaaa atatgtggct catgctttcg
    25021 acacctagat taaccctagg tggctggatt gggcatgatg ataatcattc attgtcacgt
    25081 agagcaggtt attctaataa ctctaattac atggctcatc tggtaaatgc gattcagcaa
    25141 gcttccccaa gcatttgggg gaacgagcgc tttgctttag atcctagtgt agtgaaatcg
    25201 gaagtcttga aatcaacagg tcaaaaacca gggaaggttt ctgttgaagg aaaagaagta
    25261 gaggtcacag gttcgactgt taccagctat tgggctaata aggcaggagc gacagcgaca
    25321 agttatcgct ttgctattgg tggaagtgat gcggattatc agaatgcttg gtctagtatt
    25381 gtggggagtc taccaactcc atccagctcc agcagttcaa gtagtagttc tagcgatagc
    25441 agtaactcaa gtactacacg accttcttct tcaagggcga gacgataatt ctctaaatga
    25501 agtggccaat caatggattg ccactttttt cttttcgtgt tacaataagg gtatgaatca
    25561 gtatcagaaa aagattgtta acggaaaaat ttattcgctc ctatccggct taatatgggg
    25621 aatctgtgga attttaggag agtacttctt tactcattat caggtgtctt cgggctggat
    25681 tacctctatg cgtttgacac tggcagggag tcttgtactc atttggtctg caatacaatt
    25741 aaaatcgcaa gtgctagata tttggcgaga caagaaaaat tacctgccct ttttagccta
    25801 tgctattttg gggatttttt cagttcagta ttttttctat ctctgtgtag aatactcaaa
    25861 tgctacgaca gcaactattt tacagtttat tagccctgtc tttatcctct tttacaatcg
    25921 cttggtttat caaaaacgag cgtcaaaaag cgctgttttc tatgttttgg ttgccatgct
    25981 gggtgtttgc ttgatggcga caaagggaga cctctctcag ttatccatga cgccgctagc
    26041 tcttataaca ggtttgctga gtgccatggg tgttatgttt aatgttatct tgccccaacc
    26101 ttttgctaag cgttatggtt ttgttcctac ggttgggtgg gggatgattt tggcaggttt
    26161 gtttagcaat gtcctctcgc cggtttatca gctttccttt actcttgata tttggagtat
    26221 cttgatttgc ctcattatcg ctttctttgg aacggctttt gcttttttca tttccatgaa
    26281 ggctgtgtcc ttggtttctc ctttggtggt ttccgttatc agtgccagtg aacctctctc
    26341 ttctgctctc ttgagtgttt tgttcttagg attagtagtg gattggtccc tccttctagc
    26401 tatagccttg attattttac ccatgatatt tttgtctata gaagaagcga aagaaagtag
    26461 ataaaaagtc ttttcttaat tctaaaagtg tgctatacta gaatagtcaa taaaacacgg
    26521 ggagatagtt gtgaagagtg tttttaggtt gtatcggtag gggcttgctt ttaccgacag
    26581 cacgttttat ctcacatccc taaaaaatca tacctaaaaa cagacaaaaa ggtttcaaat
    26641 ttgaggcctt ttttggtaga ataggtatca ttataacgaa ccaggaggca cctatgactg
    26701 ctacaaaaat gaacgctcaa gaaattatcc aatttatcgc caatgctgaa aagaaaacca
    26761 gtgtcaaagt aacctttgag gggcaactcg caactgctgt gcctagctct gttgtcaaac
    26821 taggaaatgt cctattcgga gactggaagg acgtggctcc gcttcttgaa ggtttggtag
    26881 aaaatcaaga ttatgttgtc gagcaagatg ctcgtaattc tgcagttcct ttgctagata
    26941 agcgtgctat caacgctcgt atcgagccag gtgctattat ccgtgaccag gtggaaattg
    27001 gtgacaatgc tgttatcatg atgggagctg ttatcaatat cggtgctgaa atcggtgctg
    27061 gaaccatgat tgacatgggt gccatccttg gtggccgtgc catcgttgga aaaaatagcc
    27121 acgttggtgc aggtgcagtt ttggcaggtg tgattgagcc agctagtgct gaaccagtcc
    27181 gtgtcggaga caatgttctt atcggagcta atgcagtggt tatcgaagga gtccaaatcg
    27241 gtagtggttc agttgtcgct gcaggagcta tcgttaccca agatgtctca gaaaacgtgg
    27301 tagtagcagg tgttccagct cgtatcatca aagaaattga tgcccaaacc caacaaaaaa
    27361 cagcgctaga ggatgcgctt cgtaccttgt aattgtaaaa gtaaaaaaga ggcggaaccc
    27421 tttttccagc ctctttctgc tatatcggag gacagataga tgttagattt gattcagact
    27481 agacgagctt tacaccagat tccagagatt ggcttggagg agttcaagac tcaggcttat
    27541 ttgctggatg tgattgagaa attgactacg ggcaaggatt ttgttcaaat tcgtacttgg
    27601 cggacaggga ttttggtcta cctgcaggga agtcagccgg agcgaaccat tggttggcgg
    27661 acagatattg atggcctgcc tatcgtcgaa caaacaggtc taccttttgc ttctcaacac
    27721 caaggtcgca tgcatgcctg tggccatgat tttcatatga ccattgcctt aggctgtctt
    27781 gagcgcgccc ttgaggagca acccaagaat aatctgctct ttctatttca gcctgctgaa
    27841 gaaaatgaag ctggtggtat gctcatgtat gaggacggtg cttttggaga ttggttgcca
    27901 aaccaattct atggtctcca tgttcgtcca gatttgaaag tcggccagat tgcgactaat
    27961 actcatacac tctttgcagg aacttgcgag gtgaagatcc atttcaaagg caaagatggc
    28021 cacgcagctt ttcctcatga agccaatgac gccttggtgg cggctagtta ctttgtgacc
    28081 caagtgcagt cagttgttag ccgcaatgtc aacccaatcg agggagcagt ggtgaccttc
    28141 ggcatttttc aagctggaac caccaacaat gtcatcacag acacagcttt tttacatgga
    28201 actattcgtg ccttgacaca ggacatgagt cttttggtgc aaaagagggt aaaaacagtt
    28261 gcagaagggg ttgcagctgc ctttgatatg gaagtcgaag tggaactcaa acaaggtgga
    28321 tacctacctg ttgagaacaa tccagccttg gcgcgtgaac tgatggactt ctttgacgaa
    28381 aaagacggaa tcgagttgat tgatatcgaa cctgctatga caggcgagga ctttggttat
    28441 ctcctttcaa aggttgatgg cgtgatgttt tggctaggta tcgatagtcc ctacgctctt
    28501 catcactctc aaatgagtcc taaggaagaa gtcttagcta ttggggtggc tgcggtgtct
    28561 agtttcctga aaaagaaggc agcagaatag agggcttgtc tatgaaatcg gaattacgca
    28621 agcaagtctt gcatgaaatg aaggctttat ctcaagaaca aaaacaggct atagaccaag
    28681 ctttaaccga gcgaatttta caacacccct tttatcaaga agccaaggtc atcgcaacct
    28741 acctctcttt ttctcatgag tttcaaacgc gggaactgat tgagcaggcg ctgaaggacg
    28801 gcaagaaggt tttaataccc aaaacttatc ccaaggggcg catggacttt gtagtctatg
    28861 atccgcagca gttggtaaaa actgcctttg ccttactgga gccacaggga gatttggaag
    28921 tggtggatgc atctcagatt gatttgattc atgttcctgg cctggctttt acgacggatg
    28981 gatatcggat tggatatggt ggaggttatt atgaccgcta tctggaacat ttttctggtc
    29041 atactttgag tacggttcat ccttgtcaaa ttcaggactt tatacctgaa aaccatgata
    29101 ttcctgttca ggaggtatta attgatgaag gaaatctttg atagacgtta ccctgtgacg
    29161 agtttcttcc tcttagtgac ggccttggta tttttactaa tgttggtcac tgcaggcgga
    29221 aactttgaca gggcagatac attatttcga tttggagcca tgtatgggcc agctattcgc
    29281 ctctttcccg agcaggtttg gcgtctcttg tctgccattt ttgttcatat tgggtgggaa
    29341 catttcattg ttaatatgct ttcactttat tatcttggaa ggcaggtaga ggagattttc
    29401 ggttctaagc agtttttctt tctctatctt ttatcaggaa tgatgggcaa tctctttgtt
    29461 tttgtattta gtcctaaatc cttagcagca ggcgcctcta cctctcttta tgggctattt
    29521 gccgcgatta ttgttcttcg ctatgcaact cgcaatcctt atatccaaca gctagggcaa
    29581 tcctatctga cactttttgt ggttaacatt attggaagtg ttctgattcc aggaatcagc
    29641 ctagcaggcc atatcggtgg tgcagttggt ggcgcatttc tagcagttat ctttccagtt
    29701 agaggagaaa aacggatgta taacaccagc cagagattag gagcggtagt cttgttcgta
    29761 ggactcgcca ttttgctttt ctacaaggga atgggaatgt gacgaagttg aaagaaaatt
    29821 tgtgtaatga aaaaagataa ggcatttttt gaaccttatc tttttatttt attttattcc
    29881 ttctcagtca attcttttcc aagttggatg aggtaattct tcaaatcatc tttgacttgt
    29941 gggtgtttga gggcgtagtc gatggatgtt ttcatgaagc caaacttgtc tccgacatcg
    30001 taacgagccc cattgaactc acgagcaaat acacgttgtg ttttattgag ggtgtcgatt
    30061 gcatctgtca gctgaatttc atttcctgca cctggagctt ggttttcgag gatttggaaa
    30121 atttcaggcg tgaggaggta gcgtccgata atagcaaggt cgctaggagc gtcctctgga
    30181 gctggttttt caacaaaggt ttcaacactg taaagaccat cttttccttc gccttgcgga
    30241 gcaataaccc cgtaagcaga tacttcgtca tgagggactg gcatgacagc gatagtagac
    30301 gcgtgggtac gctcgtagtc atccatgagt tgttcggtaa gtggaacagc cttttcgtct
    30361 gtgatatcca tcaagtcatc accaagcata acgacaaaag gttcatttcc gacgaaagcc
    30421 ttggcttgca aaacagcatc tccgagaccg cgtggatgag tttggcggat aaaatgcagc
    30481 cgcatgtcag ttgttttatc aactagcttc aaaagatctg ttttcccttt ttctttgagg
    30541 ttatattcca attcgaagtt tgaatcgaag tggtcctcaa tagaacgttt tgatttacca
    30601 gtgacaacta gaatatcttc aatacctgat ttgagagcct cttccacgat aaactggata
    30661 gttggtttgt ctacgattgg caacatttct ttggcaaggg ccttggttgc tggtaaaaat
    30721 cgagttccta gtccagcagc agggataact gcctttctaa cttttgatgt cataaaaatc
    30781 ctttctatag agggttaaga ccactcattt tctgctttaa attcattgtt catgatgtca
    30841 taaatggcat ctttgatatt ggttccgtga taaataactt ggtaaatagc ctgtgtaatg
    30901 ggcatataga ctccaagttc ttgggctagt tcataggctg ctcgagtcgt tgaaattcct
    30961 tcgattacca tgcccatatt agcttctata tcagctaggg attctcctcg tccgagagca
    31021 tctccagctc tccagttacg agagtggatg gaagttcccg ttacgatcaa atctcccaca
    31081 ccagataagc cgctataggt caatggactg gccccgagtg ctacccctag gcgggtgatt
    31141 tctgctaaac ctcgagcgat gatggctgcc ttagcattat caccaaatcc aagaccatgt
    31201 aaagctccag caccgacagc aataatattt ttaagagcac cagcagtttc aaccccgata
    31261 acatccgtat tggtataaag tcggaagtag tgattactaa atagctcctg aacgtattga
    31321 gctgtttgta aatctttaga agcagcagtt attaaagtta ggtcacgcac aatggtctct
    31381 tctgcatgac taggccctga aacaacgacg atatcactac ggagatgttc aggaatttct
    31441 tcttcaagaa tggttgataa tcgtttatgg ctatcaggtt ctaatccctt tgatgcgtgc
    31501 atgatgataa ccttatggtc caaggtttgt gcaacttgct gggcaacaag tcgtgtcact
    31561 tttgttggga caacaaacaa aatcgcatcc acatttttca atgtttctgc taagtcggtg
    31621 taggcaatga tattttcgtc tagaacgaca tctttaaagt agtgcttatt agtatggtgt
    31681 gtattaattt cattgatttg ctcgggaaga tttccccaaa tacgtacctc gtgtccattg
    31741 tcatttaaga cttgtgaaag ggcggttccc caagaaccag gccccaagac ggcgacggtt
    31801 tgtttttcca tgatttcctc cttgatagat ttctttctct tattctatca tattctaggg
    31861 gattttgcac ttgcattact ggggtatggt ggaaaaatac acaaagaccg agatgaaggg
    31921 ataaactctt agtctttagt taaagtgaga agaaatctga tagatagttt gattcagtat
    31981 atgcttataa tagaggtaaa caactcagga agttcttgta ggttgcgaga gtttggcgaa
    32041 aaaattaaaa gattacgttt ggctaaaaaa atcagtcgtt cagaattttg tggtgatgag
    32101 tctgaattaa gtatccgtca attaattaga attgaaaatg gagaatccag accaacacta
    32161 acaaagttaa aatatattgc tgaacgtttg gaggttgaag attacaagtt gatgccaagt
    32221 tatatagagt tggataagga atacctagaa ttgaagtatt tcttgatgag gactcctaca
    32281 tacgaagatg aaactatcgc ccaaaagaaa gagagtgttt ttgataagat ttttgaagag
    32341 tattatgata ggctacctga ggaagaaaga tttatcatcc caaattattc atatctagca
    32401 ctagcgaact acacagttca aaaattacca gaaaagctag ttgaaatact gtccttctgg
    32461 tgattttgct taatttctga tgttcgagac atgattgagg agatttgtct tgattttttc
    32521 ctaaaaaagg gcagactcct cccttggttt gatgctcgat ttttaaaata aggtgactta
    32581 ttctgttagg cagttttccc ctgccctcta gctctatatg gaacagggag tatcagattt
    32641 atctggcaga ttgtatcaaa taaggcttga aactgttttg aataggcgta tagacttgag
    32701 aatttgagaa tatgtcgtct agcagtagag acatattttc cggcaatatg aaggaagaga
    32761 cgtcgaaaac gcttgatagt caaggccttt acttcatcac cagctagctg ctttaaaaag
    32821 aggtagagat tataagccag acagcccatc atcatacgta cttcattctt aatcatggtc
    32881 gaactatctg tcttatcccc aaagaatcct gctttccttt ctttgataaa gttttcagca
    32941 ttccctcgtt cacgatagaa ttgaaacagc tcctcactat ctacactgct taaattggtc
    33001 aaatgaactt ttttacaacc ataaaaaaga gcacttaatc gtactcttct cttttaagga
    33061 ttagttgaat taattcaaat ccactagttc tcctgtttct aggtagacta ggcgttcaca
    33121 aatgttagca atgtaatcag cgaagcgttc cagatgccct atgatataaa ggtattgagt
    33181 tccattggga attgaggttt cttggtcttt cataagtcca atgatttcct ttgataaggc
    33241 ataataatat tggtcaatct gttcatcttt ttgagcaata ctaatagctt ttgaggcttg
    33301 gtgcaaagga aaggcaacca ataaatcggc tagcatgctg agggataatt tacccatttg
    33361 gtgtaactgt tcttcgtcag gggctagttg attttctttt agttgcaaaa cagctttggc
    33421 aatgcctgcc atatggtctc ccatacgttc aaggtctgaa caagaagaca tgatgctaat
    33481 cacaaatcga aggtcagaca cttgtggctg ctgcaaggcc aacaaacggg cacaggtcaa
    33541 ttcgatagcg ctttgacctt ggtttatagc atgatcctta ttgataatta gctctgccat
    33601 ctccttgtct ttggaggcta aggccagtaa ggcttttgaa gctgtttcaa ggacaagttg
    33661 ccctagtcct aaaaaggatt gttctaattc atgcaattct aagtcaaatt gatttctcat
    33721 aggtttttcc ttccttatcc gaaccgtcct gaaatatagt cttctgtgcg ctgatctttt
    33781 ggattggtaa acacgtcaac ggtatctcca aattcgcaaa tttctcctgt taagaaaaaa
    33841 gcagttttat ctgaaatacg tgaagcttgt tgcatgttat gggtaacaat gataatcgta
    33901 taatcctttt ttagttgctg aatgaggtct tcaattttta aagtggagat agggtctaag
    33961 gctgaagtcg gctcatccat taacagaata tcaggttcta ctgctaaagc tcgcgcaatg
    34021 caaaggcgtt gctgctgacc gccagataag gacatggcac tctttttaag atcatctttg
    34081 acttcttccc aaatggctgc cccttttaaa gatttctcca ctaaggcatc taattgtttt
    34141 ttgtctcgaa taccatgtgt ccttgggcca taagccacgt tatcatagat agacatggca
    34201 aagggattag gctgttgaaa aaccatccct acacgcttac gtagctgatt aaggttgaat
    34261 ttgctactat aaatatcttg ctcatctaag aggacttggc cttcaatatg gcaagaagga
    34321 accaaatcgt tcatccggtt aagggttttt agaaaagttg atttgccaca accagatggg
    34381 cctatcaagg cagtaatctg tctttctggt aattgaatcg aaatattttt taaggcttga
    34441 aaatccccgt aaaataagtc taggtgtctg actgaaaatg ttcccatact aggaagctcc
    34501 tttcacaagt tttcgagata ataagcttga tagagtattt atcattaaaa cagtaataat
    34561 caaaatcaca ccggtagcat aggcttcatt gacatgtagc ccctcacttg acagcatata
    34621 catatgcagg gctagagaac gacctgaaga catgagacta cttggcgtat tggtagaggt
    34681 acctaatgta tacatgaggg cagctgtctc accaacgata cggccaatag ctagtatcac
    34741 tccagctaaa atacctggca tggcaactgg tagaacaatt ctaaaaacag tccgtaactt
    34801 acctgcccca agtccataac ttgcttgacg catgctatca ctaacagata aaagggcttc
    34861 ttctgttgag cgaataatga ctggcaacac catgatgact gaggttaaga ttcctgataa
    34921 cagagagtat tgaaaaccta agaagactac aaagaagagc atgccaaaca gaccaaaaac
    34981 aatggaagga atcccagaca aggtatctga ggccaatcgc atgattttaa cacaaaggga
    35041 atcttttttt gtatattcca caagataaaa accagcaaaa atccctatgg gcaaggctaa
    35101 aagaagagca ccaaagacca gaataacggt ggaaataatc gctggcataa gggaaatgtt
    35161 ctcagaagta taagtccaag aaaagaggga tagacttaga tgaggtaaac ctttgatgag
    35221 gataaaacca atgattaaaa agagagagcc aaaggttaaa gctgaaaaac aataaacgag
    35281 aagttttagc aggtatttac tcataagatg attttccttt caagtaggca aagtaggcat
    35341 taatcaagag aataaggaaa aagagaactg ctgaggttgc aataagggct tccctatgct
    35401 gacctgatgc gtaagccatt tccagaacaa tattggttgt taaggttctg gttcctgaaa
    35461 agagtccact tggaataatc ggctggttgc ctgccaccaa aataactgcc atggtttcac
    35521 ctactgcgcg accgatgcct aaaataactg ctgataaaat accagatctc gcagctggca
    35581 agatgacact aaaaatactc cgttcatgac tagctcctag agccaagcta ccagaataat
    35641 acgttttggg aactgttcgg atagcagatt ctgacaaact gataatggtt ggcaaaatca
    35701 ttattcctaa tagtaacgaa gcggttagga cactcatgcc atttcctaaa aagcttctaa
    35761 tccaaggcac caataattgt aggccgaaaa aaccataaac aatagatgga atggccgcca
    35821 tcaagttgat agctgatttt aagaagccat agacgggctt tggacaataa taaaccataa
    35881 acaccgatgt caagatgcct gttggcaccc caatcacaat cgctcctaag gtaattaata
    35941 aggaaccaac gatcattggt aaaataccat agcttgccgg aatgttcgtt ggcgaccaat
    36001 cactgcctaa taaaaaacgg gcaaagccgt agttagccat gaaaggtaag ccattactaa
    36061 aaataaagaa acagattagc aaaatagcta caacagctac tgttgcactc atgaaaaaaa
    36121 ttgccctaaa aactgcttct ttgaaggctt gttttatcac atcttgtcct ttctagtgaa
    36181 gaaagtaagg gagatacgac acctccctac ttgccttctt tatcttattg tacgatgaaa
    36241 cgtctgcatc tctttagaga tttatggagc aaacatttta tttaatcttg tcccaggtgg
    36301 ttaatttgcc actaaaaacg tctgcaagtt cagccatact gacttggctt gccttattgt
    36361 cattattgac cacaacagca ataccgtcta aagcaatagc atcatgggtg agactcttac
    36421 cttcttcagg agttaattcc ctagaaacca taccaatatc agcggttttc tccttaacag
    36481 cggtaatacc tgctgaagac ccattagagg taatatcaat cgtaacttct ggattttctt
    36541 ttttataagc ttctgctaat ttttccatta aagaagatac tgaagtggaa cctacaacag
    36601 acaacttgcc tgataagtgt tggcttgtat attctgtggt ttcagtttta gcttcaataa
    36661 atttattatc tgtgaccact tgttgacctt gtttggagtg gataaagctg ataaaatctt
    36721 gacctagctt ggaaagatta gaagaccaaa caatgttgaa gggacgttga agagggtatt
    36781 caccatctaa aactgtgtct cgactagcct tgacaccatc aatctctaaa gccttgacag
    36841 atttcgttaa agatcccaag gagatgtagc cgatagcatt agcattccct tgaactgctg
    36901 agagaacacc ttctgtacta ttttgaatca cagctgtttt ggcagtgttg tcaatttttt
    36961 tatcaccgtc ttttttgaga atccctgtga tttctgtgaa ggcaccccgt gttccagagc
    37021 cattttctcg tgaaatcacc tcaatcgttc ctgaagctga ctgtttggaa gcagctgact
    37081 gattgccaca ggcaacaagc ccaaatcctg ataagccaat ggctgcaaga gtaagcattt
    37141 ttttgaattt cataataatc acctttatct ctatgtattt ttcttgtgta ggcttactac
    37201 atttatagtc taacaagtct ttgtaaaggt ttatccctga ttcatgtaaa gattgtgtaa
    37261 agaatcaaaa aaagccactt ttgaaaaatg gctgctccta aaaatagctt taaaaattat
    37321 tagtcctgtg cgaaagattg gttaggaaga aaaatcgtga agcaactgcc tctgccaagc
    37381 tgactcgtca ccgtgacttg gccacctaat aattgactga gttctttgac aatggcaagg
    37441 ccaagaccag tgccaccagt ttgtctgctt cgacctttat taactcggta aaaacgttca
    37501 aaaatacgat cctgctctaa ttgactaata ccaatccctg tatctgatac agaaatctta
    37561 atgccttcgt tcaccttttg ggtcttgacc tcaatttttc ccccttgttc agtgtaacgg
    37621 atggcattgg ataaaagatt gagtaagatt tgggaaagta attgactatc tgatacgagg
    37681 gtgacatcat ctggcacctg cacctttagc tgtaaatcct tcttcttgag ctgaggttgc
    37741 aagctttgag tcaaatcctg tacaaattca gccaaagaaa gggtcgtcca ttgtataggc
    37801 atttgttgag ccttagataa ggtaagaaga tgctcaacaa tatgctcaag acgcaaactt
    37861 tctttgtaaa taatgtctag aaagtcatcc ttgagcgctt cttcttcagc tgacatcccc
    37921 ttaatggttt cagcaaagcc cttaattgaa gtaactggtg tcctcaattc atgggaggca
    37981 tttgagacaa aggctaaatt taacttttca taagttctaa tcgttgttaa atcatatagc
    38041 aagacgagca cagcttccac agattgggtg gggctaaaaa cgggaactgc tgtcacttct
    38101 aaaatcaagt caccctcatg aaacccactt acttcttgtt ttaaccttgt tttttgatca
    38161 aaggcttggt gaactaaatt ccgaatatcc atccgtttga ggtcatcaag tgaacttatg
    38221 tcgccgtcca catcgggaaa ataatgaggc agagagcgac tggataataa catctgacct
    38281 tgagcggaaa ctaaaaacgt ccccatggtt aggtgcgaca gaagaacctc cattgtttcg
    38341 gctaggtcct tgtattgctg atcctgttgg gagactttgg tttttaggcc agacacatac
    38401 tgagccaaag actttaagtc ttcttgccct ttttctaaaa agtattcact actggtcaag
    38461 agaggttggt gcaaggtctc aaaagcaact tcccatttcc aaaggcaaaa gagccagtag
    38521 ccaccttgtc ccaaagaaag ggctagaaga aagagaccga tgcctttact gatccaagtt
    38581 aatgccatcc ctgcaatcag aatgaggcta acacttagat tgactagcca aaattgaagg
    38641 tagcgtttca tctataactc cttgaactta taaccataac cccgaatggt tcgaataaat
    38701 tgaggggctt taggattgtc ttcaattttt tccctcaact taccaatatg aacgtccacc
    38761 aaacgtgttt cctgcccaaa gtcatacccc cagatacgtt ccaaaagacg ctctctagtc
    38821 agtgtcatgt tgggatgttt cataagatag agcaagagtt caaattcttt tggggtcaaa
    38881 ctcagtaact tattcgcctt gtagacttca tgacgctcag ggtatacttt caaggtccca
    38941 aatagccaag aatcgtcagc gatattatct gaatcatctc cttcttgttc tcctttagtt
    39001 cgcctgagga cagccttgac acgcgccagc aattctctag ggctaaaagg cttggtcagg
    39061 tagtcatcag cccctaattc caaggccaaa accttatcaa attcatcact tttcgcagaa
    39121 accatcataa ttggagtttt gacgcctttg gctctcagcc gcttacaaac ttccatgcca
    39181 tctaattgtg gtaacatgat atcaagcaag ataaaatcaa agggttctgt ttctgccaaa
    39241 gctaaggcct tccgtccatt tgttaccaat tgagtagaaa agccttcctt acttaaatgg
    39301 tagtcaagca atttcagaat gtgttcttca tcatccacta ataagacttg ttttgtcatc
    39361 tattatctcc tattggtaac attataacac aattatcaga aatcctaaca ttgctaaatc
    39421 agattaaatt tgcttatcaa gactagtatc tggtcaaacg ctcaatcatc tccttgtgct
    39481 ctggataggt cgccagtaga tctacccttt caaataattc aaaatcctca aattcaaaac
    39541 caggagcaac aagacaagaa accagagcat aatccttatc aactgttgat ccccaaatag
    39601 tgcccttagg aacacagtag tgaagttgtt gccctttgga tatgtccagg cctaaagtga
    39661 ctgcttcgta gtgaccatct gctgtaatca tgtgaacagt aagtggggat cctgcatgaa
    39721 aataccagat ttcatctgct gtcaatcggt gaaaatgtga aggattcgtt tcttctaata
    39781 agaaataaat actggtataa agcgcccttc ccttaccagc aaggtttata gtgtctgaag
    39841 ctttttttgt ttgtctaaaa tagccacctt caacatgggg agctaactct agagttctta
    39901 tcaagtcttc tttatccatc ggggccaatg ggttgaagta actcttgttc aaagtggttt
    39961 tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt
    40021 acaaatcaat cgccctaaaa aaagaattag cgaatgattc tggtaaaaag aaatttcacg
    40081 ctatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctatt gccatgatat
    40141 gaagttgttc aaaatgagtt gcagaaatat cggacaagct ggtaaaatct tggctgacag
    40201 tgattatcaa gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa
    40261 actcaagccg ctaacagctg aagataaagc ctataaccat gcgctatcta aggaaagaag
    40321 caaggttgag aacatctttg ccaaagtaaa aacgtttaaa atattttcaa caacctatcg
    40381 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga
    40441 actaggattc tagttttgca ggaagtctat tatttggtta ggtgaattag tgaagtgttt
    40501 aggcaagtgt ctctggttac gacgtcatgg cctctaaatc gattatattt aggggtcatg
    40561 actagtgaag cagttagcta gttcgcatat aagcggctag cgtctaacaa ttaggaactt
    40621 tagttccaat aactttaaga ttacgacgtt ttaggacata aatcgatcat atttatgtcc
    40681 taaaactagt gaagcgccta gccaaagtcc gaataggatt tggcgttagt tacttagatt
    40741 gctttgcaat caagtaactt tggcgattta catcttctct ggcgcttcta ctccaagcaa
    40801 gcgaagggct tctttgagaa cgactgcggt tgcgtagctg agggctagac ggctgtcgcg
    40861 ttctgggctt tcatccaaga tacgtgtatg tgcatagtat ttgttaaagg attgagccag
    40921 gctaattgca aatttagcaa tgatagaagg ttcaaagtta tctgccgcac ggttgataat
    40981 acgtgggaag tcttgaatga gtttaatgat ttcccagctt tcagtatcat tcaagctata
    41041 gttgccagct gtttctggtt tgaaatcggc tttgcgtaag atagattgga tacgagcgta
    41101 ggcatattga acgtaaggtc cagtttcacc ctcgaaggat accatagctt ctaggtcgaa
    41161 gtcgtatcca tttgtacggt cagttttgag gtcatagaat ttaatggctc caaccccaac
    41221 agcatgtgct acttggtctt tgttttctag ttcaggattt ttagcctcga tttggacctt
    41281 ggcacggcta acagcctctg caacagtagg ctctagcaag atgacattcc ctttacgagt
    41341 agagagcttc ttcccttctt ttgtaaccag accaaaagga acgtgggtaa tgtcgtcact
    41401 ccaatcgtag cccatctctt gcaagacagc tttgagttgt ttaaagtggg cagattgctc
    41461 ttgaccaacg acgtagatag atttagcaaa ttggtattca tttttacggt agagggctgc
    41521 agccaagtca cgtgtgatat agagagttgc accatcagac ttcttgatga gggctggatg
    41581 ttcaattcca tatttctcaa gattcacaac ttgggcacct tctgattcaa gaagtagtcc
    41641 tttttcagaa agaatgtcta caactgcatc catcttatca ttgtagaagg cttctccgtt
    41701 atagctgtca aattcaacct tcaattcatt gtaaaggcgg ttaaattcca ctaaactttc
    41761 atcgcggaac cattgccaaa gagcgagagc ttcctcatct ccattttcaa gtttacggaa
    41821 ccattcgcgc gcttcttcat ccaagctagg gtcattttca gcttcagcgt tgatgcggac
    41881 atagagttta aggagttcat cgattggatg agcttttaca gcttcttcgt cgccccattt
    41941 tttgtaggca acaatcaaca tcccaaattg tttaccccag tctcccaaat ggttgacctt
    42001 gaccgtttga taaccgattt tttggaaaat atgtgacaag ctatctccga taacagttga
    42061 acgcaggtgg ccaatagaaa atggtttagc gatattcgga ctagacatgt cgataacaac
    42121 attttcttgt ttgccaatat tttggtcagc atagtgttct ttttcagtgg taacagcttg
    42181 caatacttga gcagaaatgg cagatttatc aaggaaaaag ttaacgtaag gtcctgttgc
    42241 gacaactttt tcaaaggctt ggctgttcat tttttcagcc agttcagccg caatcatttg
    42301 tggtgcttta cgttcgactt tggcaagaga aaaagcaggg aaagcaatgt ctcccatttc
    42361 tgagttttta ggggtttcca gtaactttaa aatagcctct tggtccaggc tatcaatgat
    42421 gctagataat tcgctagcaa tcaattcttt tgtattcatt aagagctcct ttttggactt
    42481 ttctactatt ttatcacaat tttaaagaaa gaagaaaaaa tttttgaaat ctcctgtttt
    42541 tttggtataa tatggttata aatatagtta taaatatgca cgcaagagga ttttatgaga
    42601 aaaagagatc gtcatcagtt aataaaaaaa atgattactg aggagaaatt aagtacacaa
    42661 aaagaaattc aagatcggtt ggaggcgcac aatgtttgtg tgacgcagac aaccttgtct
    42721 cgtgatttgc gcgaaatcgg cttgaccaag gtcaagaaaa atgatatggt gtattatgta
    42781 ctagtaaatg agacagaaaa gattgatttg gtggaatttt tgtctcatca tttagaaggt
    42841 gttgcaagag cagagtttac cttggtgctt cataccaaat tgggagaagc ctctgttttg
    42901 gcaaatattg tagatgtaaa caaggatgaa tggattttag gaacagttgc tggtgccaat
    42961 accttattgg ttatttgtcg agatcagcac gttgccaaac tcatggaaga tcgtttgcta
    43021 gatttgatga aagataagta aggtcttggg agttgctctc aagacttatt tttgaaaagg
    43081 agagacagaa aatggcgata gaaaagctat cacccggcat gcaacagtat gtggatatta
    43141 aaaagcaata tccagatgct tttttgctct ttcggatggg tgatttttat gaattatttt
    43201 atgaggatgc ggtcaatgct gcgcagattc tggaaatttc cttaacgagt cgcaacaaga
    43261 atgccgacaa tccgatccct atggcgggtg ttccctatca ttctgcccaa cagtatatcg
    43321 atgtcttgat tgagcagggt tataaggtgg ctatcgcaga gcagatggaa gatcctaaac
    43381 aagcagttgg ggttgttaaa cgagaggttg ttcaggtcat tacgccaggg acagtggtcg
    43441 atagcagtaa gccggacagt cagaataatt ttttggtttc catagaccgc gaaggcaatc
    43501 aatttggcct agcttatatg gatttggtga cgggtgactt ttatgtgaca ggtcttttgg
    43561 atttcacgct ggtttgtggg gaaatccgta acctcaaggc tcgagaagtg gtgttgggtt
    43621 atgacttgtc tgaggaagaa gaacaaatcc tcagccgcca gatgaatctg gtactctctt
    43681 atgaaaaaga aagctttgaa gaccttcatt tattggattt gcgattggca acggtggagc
    43741 aaacggcatc tagtaagctg ctccagtatg ttcatcggac tcagatgagg gaattgaacc
    43801 acctcaaacc tgttatccgc tacgaaatta aggatttctt gcagatggat tatgcgacca
    43861 aggctagtct ggatttggtt gagaatgctc gctcaggtaa gaaacaaggc agtcttttct
    43921 ggcttttgga tgaaaccaaa acggctatgg ggatgcgtct cttgcgttct tggattcatc
    43981 gccccttgat tgataaggaa cgaatcgtcc aacgtcaaga agtagtgcag gtctttctcg
    44041 accatttctt tgagcgtagt gacttgacag acagtctcaa gggtgtttat gacattgagc
    44101 gcttggctag tcgtgtttct tttggtaaaa ccaatccaaa ggatctcttg cagttggcga
    44161 ctaccttgtc tagtgtgcca cggattcgtg cgattttaga agggatggag caacctactc
    44221 tagcctatct catcgcacaa ctggatgcaa tccctgagtt ggagagtttg attagcgcag
    44281 cgattgctcc tgaagctcct catgtgatta cagatggggg aattatccgg actggatttg
    44341 atgagacttt agacaagtat cgttgcgttc tcagagaagg gactagctgg attgctgaga
    44401 ttgaggctaa ggagcgagaa aactctggta tcagcacgct caagattgac tacaataaaa
    44461 aggatggcta ctattttcat gtgaccaatt cgcaactggg aaatgtgcca gcccactttt
    44521 tccgcaaggc gacgctgaaa aactcagaac gctttggaac cgaagaatta gcccgtatcg
    44581 agggagatat gcttgaggcg cgtgagaagt cagccaacct cgaatacgaa atatttatgc
    44641 gcattcgtga agaggtcggc aagtacatcc agcgtttaca agctctagcc caaggaattg
    44701 cgacggttga tgtcttacag agtctggcgg ttgtggctga aacccagcat ttgattcgac
    44761 ctgagtttgg tgacgattca caaattgata tccggaaagg gcgccatgct gtcgttgaaa
    44821 aggttatggg ggctcagacc tatattccaa atacgattca gatggcagaa gataccagta
    44881 ttcaactggt tacagggcca aacatgagtg ggaagtctac ctatatgcgt cagttagcca
    44941 tgacggcggt tatggcccag ctgggttcct atgttcctgc tgaaagcgcc catttaccga
    45001 tttttgatgc gatttttacc cgtatcggag cagcagatga cttggtttcg ggtcagtcaa
    45061 cctttatggt ggagatgatg gaggccaata atgccatttc gcatgcgacc aagaactctc
    45121 tcattctctt tgatgaattg ggacgtggaa ctgcaactta tgacgggatg gctcttgctc
    45181 agtccatcat cgaatatatc catgagcaca tcggagctaa gaccctcttt gcgacccact
    45241 accatgagtt gactagtctg gagtctagtt tacaacactt ggtcaatgtc cacgtggcaa
    45301 ctttggagca ggatgggcag gtcaccttcc ttcacaagat tgaaccggga ccagctgata
    45361 aatcctacgg tatccatgtt gccaagattg ctggcttgcc agcagacctt ttagcaaggg
    45421 cggataagat tttgactcag ctagagaatc aaggaacaga gagtcctcct cccatgagac
    45481 aaactagtgc tgtcactgaa cagatttcac tctttgatag ggcagaagag catcctatcc
    45541 tagcagaatt agctaaactg gatgtgtata atatgacacc tatgcaggtt atgaatgtct
    45601 tagtagagtt aaaacagaaa ctataaaacc aagactcact agttaatcta gctgtatcaa
    45661 ggagacttct ttgacaattc tccacttttt tgctagaata acatcacaca aacagaatga
    45721 aaaggagctg acgcattgtc gctccctttt gtctattttt taaggagaaa gtatgctgat
    45781 tcagaaaata aaaacctaca agtggcaggc cctggcttcg ctcctgatga caggcttgat
    45841 ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg ccctccttgc
    45901 tgggaaatat gaagctattt atagtatcgg ggcttggttg attggtgtgg ccgtagtcgg
    45961 tctagttgct ggtggactca atgttgtcct cgcagcctat attgcccaag gagtttcatc
    46021 cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg atattgaaca
    46081 atttaatgcg ggaaatctag tcgttcgaat gacaaatgat atcaaccaga ttcagaacgt
    46141 tgtcatgatg accttccaga ttcttttcag acttcccctc ttgttcatcg gttcgtttat
    46201 cctagcggtt caaaccttac cttctctgtg gtgggtgatt gttctcatgg tagtcttgat
    46261 ttttggtttg actgctgtca tgatgggaat gatggggcct cgttttgcca agtttcaaac
    46321 ccttcttgag cgcatcaatg ccattgccaa ggaaaattta cgtggcgttc gtgtggtcaa
    46381 gtcctttgtc caagaaaaag agcaatttgc taagtttaca gaggtctcag acgagcttct
    46441 tggtcaaaac ctttacattg gttatgcctt ttcagtagtg gaacccttta tgatgttggt
    46501 tggttacggg gcggtcttcc tctctatttg gctggtcgcg ggaatggttc agtcggatcc
    46561 gtctgttgtt ggttccatcg cttcttttgt taattaccta agccagatta tctttaccat
    46621 tgttatggtt ggatttttgg gaaattctgt cagccgtgcc atgatttcca tgcgtcgtat
    46681 tcgagaaatt cttgacgcag agccagctat gaccttcaag gatatcccag atgaagagtt
    46741 ggttggaagc cttagctttg aaaatgtgac ctttacctat ccaatggaca aggaaccgat
    46801 gctgaaagat gtgagcttta ctattgaacc tggtcaaatg gttggtgtag ttggagcgac
    46861 tggtgcagga aagtcaacct tggctcaatt gattccacgt ctctttgatc cacaggaagg
    46921 ggctatcaaa atcggaggta aggatattcg agaagtgagt gaaggaaccc tgcgtaaaac
    46981 agtttccatc gttctccaac gtgccattct ttttagtgga acgattgcag ataacttgag
    47041 acaggggaag ggaaatgcta ctctatttga aatggagcgc gcagccaata ttgcccaggc
    47101 tagtgaattc attcatcgta tggagaaaac ctttgaaagt ccagttgaag aacggggaac
    47161 caatttctct ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg tcagcaatcc
    47221 acgtattctg atttttgatg attcgacctc agccttggat gccaaatcag agcgcttggt
    47281 gcaagaagct ttgaataagg acttgaaggg gacgacaacc attattattg ctcaaaaaat
    47341 tagctcggtt gtccatgcag acaagatctt ggttctaaat caaggacgat tgattggtca
    47401 aggcacgcat gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct atgaaacaca
    47461 gaaatgaaag acaaactata agaaaagtca atagttttat ctaaactatt tcttatttca
    47521 atttgatgat ttggcgatga ttttagagca cggcaaaaag ctattgaaaa agtccatttt
    47581 ttcaaaggta atcctgtgtt aatttcagaa attacatcac tttttgttcg tcaaatggca
    47641 gctctttttt aggatataaa acagggttcg gataagtttt tttgcaaggt ggatgatggc
    47701 tacattgtaa tgttttcctt gttctaactt agtcttaaga taggccttaa aagcaggtga
    47761 aaagcgaggg catgctttgg cagcttgtat gagtacctac cgcagatgag gggaaccccg
    47821 tttgaccatt cttcctgcta aatcaatctg acctgactga taaatagaag aatccagtcc
    47881 agcgaaagct tgtaattgag caggattatc aaaggcatga atatttcgaa tctcggctaa
    47941 aatgaccgcc cctaaacgat tctcaatccc agtaaccgtc gtgatgaccg agttgaactc
    48001 agccatcaag tcattgacac attttttcgc cttgtcaatg agcctcttgt aatgtttgat
    48061 gttttcatta caagagataa aacgtttatg cgttatcaaa ctcattacca attgaaacaa
    48121 aaagctgtgg ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg aactaatcca
    48181 cagcggctta ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata
    48241 atataaggag gagtaaaatg aagacagttc aatttttttg gcattatttt aaggtctaca
    48301 agttctcatt tgtagttgtc atcctgatga ttgttctggc gacttttgcc caagccctct
    48361 ttccagtctt ttctggacaa gcggtgacgc agctagccaa tttagttcaa gcttatcaaa
    48421 atggcaatcc agaacttgta tggcaaagcc tatcaggaat catggtcaat cttggcctgc
    48481 tggttttggt tctatttatc tctagtgtaa tatacatgtg tctcatgacg cgcgtgattg
    48541 cagaatcgac caacgagatg cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt
    48601 ctttctttga ccgtcgacaa gatggcgata tcctgtctca ttttaccagt gatttggata
    48661 atatcctcca agcctttaac gaaagcttga ttcaggtcat gagcaatatt gttttataca
    48721 ttggtctgat tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc accattgcca
    48781 gcaccccatt ggctttcctt atgctgattt tcatcgtgaa aatggcacgt aaatacacca
    48841 acctccagca gaaagaggta gggaagctca acgcctatat ggatgagagc atctcaggcc
    48901 aaaaagccgt gattgtgcaa ggaattcaag aggatatgat ggcaggattt cttgaacaaa
    48961 atgagcgcgt gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt cttttccctg
    49021 tcatgaatgg gatgagcctg attaatacag ccatcgtcat ctttgctggt tcggctgtac
    49081 ttttgaatga taagtctatt gaaacaagta cagccctagg tttgattgtt atgtttgcac
    49141 aattttcaca gcagtactac cagcctatta tccaagttgc agcgagttgg ggaagccttc
    49201 agttggcctt tactggagct gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc
    49261 gacctgaaaa ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg
    49321 atttttcata cttgcctgat aaacctattt tgaaagatgt cagcatttct gcccctaaag
    49381 gccagatgac agcagttgtt gggcctacag gttcaggaaa aacgactatt atgaacctca
    49441 tcaatcgctt ttatgatgtt gatgctggtg gtatttattt tgatggtaaa gacattcgtg
    49501 gctatgactt agatagtctt agaagcaagg tgggaattgt attgcaagat tcggtcttgt
    49561 ttagcggaac gattcgagac aatatccgat ttggtgtgcc agatgctaat caggaaatgg
    49621 ttgaggtagc agcaaaagca acccacattc acgactatat cgaaagtttg cctgataagt
    49681 acgatactct tattgatgat gaccagagca tcttttcaac agggcagaag caattgattt
    49741 caatcgctcg aaccctgatg acagatccag aagttctcat tctcgatgaa gcaacttcaa
    49801 acgtagatac ggtgacggaa agcaagattc agcatgccat ggaggtggtt gtagcaggta
    49861 gaactagttt cgtcattgcc caccgcttga aaaccattct caatgcagat cagattattg
    49921 tccttaaaga tggagaagtc attgaacgtg gtaaccacca tgaacttttg aagctaggtg
    49981 gcttttattc agaactctat cacaatcaat ttgttttcga ataagaaaga agttgtccta
    50041 tgtgggcagc tttttcttgt ccataaaaaa tgtttatcac agccttaaaa aaaacatatt
    50101 agacgaaagt cattttgagt gatatgatag gactatcgtt agtattcgaa aggagaggca
    50161 tcatggctag aacggttgta ggagttgctg caaatctatg tcccgtagac gcagaaggca
    50221 aaatcattca ttcatctgta tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc
    50281 tccctttagt cattcctgtt ggtgatgagt cagttgtacg tgattatgtg gaaatgattg
    50341 acaaactcat tttgacagga ggccaaaatg ttcatcctca gttttatgga gagaaaaaga
    50401 ccgtcgaaag cgatgattac aatctggtcc gtgacgaatt tgaattggca ctcttgaagg
    50461 aagcgcttcg tcagaataaa ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg
    50521 ttgcctttgg tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg
    50581 ggacatctca ctctattgag acagtagaag gaagcgtggt ggctaagcta tttgggaaag
    50641 aaagtcaggt caactcagtc catcgtcaaa gcattaaaga tttggcacct aatttccgtg
    50701 taactgctat tgattcaaga gaccaaacta ttgaagcgat tgagtctatc gacgagcacc
    50761 gcattatcgg tttacagtgg catccagagt ttctggttaa tgaagaagat ggcaatttag
    50821 aattatttga gtatttattg aatgaactgt aacgattgga acatctggtc gttctttctt
    50881 ttattttttc aaaattttta gaatgtggca ttttacgcaa acgtttgaat tctgataaaa
    50941 attggagaaa ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat gatagaaaag
    51001 aagaaatatt ggaggaagaa catgtcacat attaaatttg attattcaaa agttttagac
    51061 aaatttgttg caccacatga agtggaatac atgcaatcac aagtaacagc agcagatgaa
    51121 ttgatccgta aaggaactgg tgctggtagc gacttcttgg gatggttgga ccttcctgaa
    51181 aaatacgacc gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac
    51241 agcgatgttt tggttgtgat cggtattggt ggatcttacc ttggtgcaaa agcagcaatc
    51301 gacttcttga atcaccattt tgctaacttg caaacaaaag aagaacgcaa agctccacaa
    51361 atcctttacg caggaaactc aatctcatct acttaccttg ctgacttggt agagtatgtt
    51421 gcagacaaag acttctcagt aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg
    51481 attgctttcc gtgtctttaa agaactcttg gttaagaaat acggtcaaga agaagctaac
    51541 aaacgtatct atgcaacaac tgaccgccaa aagggtgctg ttaaggttga agcagacgct
    51601 aacggttggg gaacatttgt tgttccagat gatatcggtg gacgcttctc agtattgaca
    51661 gccgttggtt tgcttccaat cgcagcatca ggagctgaca tcaaagctct tatggaaggt
    51721 gcgaatgcag ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga agcttaccaa
    51781 tacgcagtcg ttcgtaacat cctttatcgt aaaggctatg caactgagat cttggtaaac
    51841 tatgagccat cacttcaata cttctcagaa tggtggaaac aattggctgg tgaatcagaa
    51901 ggaaaagacc aaaaaggtat ctacccaact tcagccaact tctcaactga cttgcactca
    51961 cttggtcaat ttatccaaga aggaactcgt atcatgtttg aaacagttgt ccgtgttgac
    52021 aaacctcgta aaaacgtgct tattcctact ttggaagaag accttgacgg acttggttac
    52081 cttcaaggaa aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc
    52141 cacacagatg gtgatgtacc aaacatgtat gtgactcttc cagagcaaga cgctttcact
    52201 cttggttaca ctatctactt cttcgaattg gcaattgccc tttcaggtta cttgaatgct
    52261 atcaacccat ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt tgcccttctt
    52321 ggaaaaccag gatttgaaga attgagcaaa gaacttaacg cacgtctata atagaagaaa
    52381 agagtggttt gcccactctt tttactctct ttatccatag aaattggact cagccaagac
    52441 ttgtgatata atatagaaag caaaaaggca gacgcctaga taataggaga aactatgtca
    52501 aaagatatcc gcgtacgtta cgcaccaagt ccaacaggac tactacacat cggaaatgcc
    52561 cgtacagcat tgtttaacta cttgtatgcg cgccatcatg gtggaacatt tctcatccgt
    52621 atcgaagata ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca acttgaaaac
    52681 cttcgctggt taggcatgga ttgggatgaa agtccagaat cacatgagaa ttatcgccag
    52741 tctgagcgtt tggacttgta tcaaaaatac attgaccaac tattagctga aggaaaagcc
    52801 tataaatctt acgttacaga agaagagttg gcagctgaac gtgaacgtca agaagtagct
    52861 ggcgaaacac cacgctacat caatgaatac cttggtatga gtgaagaaga aaaagcggct
    52921 tatatcgcag aacgtgaagc agctggtatc ataccaacag ttcgtttggc tgtcaatgag
    52981 tcaggtatct acaagtggca tgatatggtc aaaggtgata tcgaatttga aggtggcaat
    53041 atcggcggtg actgggttat ccaaaagaaa gacggttacc caacttacaa ctttgccgtt
    53101 gttatcgatg accacgatat gcaaatctct catgttatcc gtggagatga ccatattgct
    53161 aatacaccaa aacagcttat ggtctatgaa gctcttggtt gggaagctcc agagtttggt
    53221 cacatgacct tgattatcaa ctctgagact ggtaagaagt tatctaaacg tgatactaat
    53281 acacttcaat ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc agtctttaac
    53341 tttattgctc ttcttggttg gaacccaggt ggtgaagatg agattttctc tcgtgaagaa
    53401 ctcattaaac tttttgatga aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa
    53461 aaattagact ggatgagcaa tgattatatc aagaatgcag accttgaaac catctttgaa
    53521 atggcaaaac cattcttaga ggaagcaggc cgtttgactg acaaggctga aaaattagtt
    53581 gagctctata aaccacaaat gaaatcagta gatgagatta tcccattgac agatcttttc
    53641 ttctcagatt tcccagaatt gacagaagca gagcgcgaag tcatgacggg tgaaacagtt
    53701 ccaacagttc ttgaagcatt caaagcaaaa cttgaagcga tgacagatga tgaatttgtg
    53761 acagaaaata tcttcccaca aattaaagca gtccaaaaag aaacaggtat taaagggaaa
    53821 aatcttttca tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca
    53881 gatacaattt tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa cattctaaaa
    53941 gaaatctcta aataaagagg atacaataat ggacatctgg gaaaagatgt acgaagaagc
    54001 acagaaacta tacaatccac atgaagtatc agactttgtt tatgccaatc atgttgtagc
    54061 tgcagtagaa gcaggagatg gacaaatatt tacaggattc tgtatggagg gaacctgtgg
    54121 tgttttccat ctctgcgcag aacgggcgac cctcttcaat atgtaccaat tttcaggaca
    54181 aactaaggtt aagaaagtat tagcctttcg agacaaacca ccttatggtg gaagttcagc
    54241 catgccttgc ggtgcttgta gagaattcct tttagagctt aatgctgaaa ataaagatgc
    54301 agaattcatg atggactata atataagaaa aacagttaaa gtcgcagaac taatccctta
    54361 ttggtgggga gaagaacgtg cttctaagtt taatgagtga tagaagagtc agtataattc
    54421 tggctctttt tactataagt tgaaaaaata aaaaaaatta gaaaaagtag ttgacaaa
//
LOCUS       DAVLWA010000005        53851 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLWA010000005 DAVLWA010000000
VERSION     DAVLWA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.005"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            53..868
                     /locus_tag="VYN80_000277"
     CDS             53..868
                     /locus_tag="VYN80_000277"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1540252.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVYDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            887..1528
                     /locus_tag="VYN80_000278"
     CDS             887..1528
                     /locus_tag="VYN80_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1540253.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGIVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1559..2560
                     /locus_tag="VYN80_000279"
     CDS             1559..2560
                     /locus_tag="VYN80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1540254.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEKV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEEQFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSSTSIENLLANAQDIIR"
     gene            2570..3199
                     /locus_tag="VYN80_000280"
     CDS             2570..3199
                     /locus_tag="VYN80_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1540255.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(3578..3963)
                     /gene="tnpA"
                     /locus_tag="VYN80_000281"
                     /pseudo
     CDS             complement(3578..3963)
                     /gene="tnpA"
                     /locus_tag="VYN80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4345..7548)
                     /locus_tag="VYN80_000282"
     CDS             complement(4345..7548)
                     /locus_tag="VYN80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1540256.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQDHKQNGDSYGYM
                     LIPNVDRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            7771..8247
                     /locus_tag="VYN80_000283"
     CDS             7771..8247
                     /locus_tag="VYN80_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1540257.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(8469..10682)
                     /locus_tag="VYN80_000284"
     CDS             complement(8469..10682)
                     /locus_tag="VYN80_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1540258.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11083..11259)
                     /locus_tag="VYN80_000285"
     CDS             complement(11083..11259)
                     /locus_tag="VYN80_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540259.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11406..11828
                     /locus_tag="VYN80_000286"
     CDS             11406..11828
                     /locus_tag="VYN80_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1540260.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            complement(12090..12482)
                     /gene="rpsI"
                     /locus_tag="VYN80_000287"
     CDS             complement(12090..12482)
                     /gene="rpsI"
                     /locus_tag="VYN80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1540261.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(12502..12948)
                     /gene="rplM"
                     /locus_tag="VYN80_000288"
     CDS             complement(12502..12948)
                     /gene="rplM"
                     /locus_tag="VYN80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1540262.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            13305..13622
                     /locus_tag="VYN80_000289"
     CDS             13305..13622
                     /locus_tag="VYN80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1540263.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(13667..14479)
                     /gene="folK"
                     /locus_tag="VYN80_000290"
     CDS             complement(13667..14479)
                     /gene="folK"
                     /locus_tag="VYN80_000290"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1540264.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(14522..15076)
                     /gene="folE"
                     /locus_tag="VYN80_000291"
     CDS             complement(14522..15076)
                     /gene="folE"
                     /locus_tag="VYN80_000291"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1540265.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(15057..16379)
                     /locus_tag="VYN80_000292"
     CDS             complement(15057..16379)
                     /locus_tag="VYN80_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1540266.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(16381..17355)
                     /gene="folP"
                     /locus_tag="VYN80_000293"
     CDS             complement(16381..17355)
                     /gene="folP"
                     /locus_tag="VYN80_000293"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1540267.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(17450..18157)
                     /locus_tag="VYN80_000294"
     CDS             complement(17450..18157)
                     /locus_tag="VYN80_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1540268.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(18210..19628)
                     /locus_tag="VYN80_000295"
     CDS             complement(18210..19628)
                     /locus_tag="VYN80_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1540269.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIYWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19818..20630
                     /locus_tag="VYN80_000296"
     CDS             19818..20630
                     /locus_tag="VYN80_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1540270.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            20969..21988
                     /gene="adhP"
                     /locus_tag="VYN80_000297"
     CDS             20969..21988
                     /gene="adhP"
                     /locus_tag="VYN80_000297"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1540271.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAFSIFDEMEKGQIQGRMV
                     LDFTH"
     gene            22221..23210
                     /gene="manX"
                     /locus_tag="VYN80_000298"
     CDS             22221..23210
                     /gene="manX"
                     /locus_tag="VYN80_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1540272.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            23238..24041
                     /locus_tag="VYN80_000299"
     CDS             23238..24041
                     /locus_tag="VYN80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1540273.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            24065..24976
                     /locus_tag="VYN80_000300"
     CDS             24065..24976
                     /locus_tag="VYN80_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1540274.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(25032..26366)
                     /gene="pepC"
                     /locus_tag="VYN80_000301"
     CDS             complement(25032..26366)
                     /gene="pepC"
                     /locus_tag="VYN80_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1540275.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(26583..27305)
                     /locus_tag="VYN80_000302"
     CDS             complement(26583..27305)
                     /locus_tag="VYN80_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1540276.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(27573..27803)
                     /locus_tag="VYN80_000303"
     CDS             complement(27573..27803)
                     /locus_tag="VYN80_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1540277.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(27813..29054)
                     /locus_tag="VYN80_000304"
     CDS             complement(27813..29054)
                     /locus_tag="VYN80_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1540278.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(29076..29228)
                     /locus_tag="VYN80_000305"
     CDS             complement(29076..29228)
                     /locus_tag="VYN80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540279.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(29249..29527)
                     /locus_tag="VYN80_000306"
     CDS             complement(29249..29527)
                     /locus_tag="VYN80_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1540280.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(29520..29783)
                     /locus_tag="VYN80_000307"
     CDS             complement(29520..29783)
                     /locus_tag="VYN80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1540281.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(29885..34276)
                     /locus_tag="VYN80_000308"
     CDS             complement(29885..34276)
                     /locus_tag="VYN80_000308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1540282.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLIMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(34380..36461)
                     /gene="fusA"
                     /locus_tag="VYN80_000309"
     CDS             complement(34380..36461)
                     /gene="fusA"
                     /locus_tag="VYN80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1540283.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPVGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(36886..37356)
                     /gene="rpsG"
                     /locus_tag="VYN80_000310"
     CDS             complement(36886..37356)
                     /gene="rpsG"
                     /locus_tag="VYN80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1540284.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(37376..37789)
                     /gene="rpsL"
                     /locus_tag="VYN80_000311"
     CDS             complement(37376..37789)
                     /gene="rpsL"
                     /locus_tag="VYN80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1540285.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            38206..42003
                     /gene="spuA"
                     /locus_tag="VYN80_000312"
     CDS             38206..42003
                     /gene="spuA"
                     /locus_tag="VYN80_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1540286.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYTSSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            complement(42103..43152)
                     /locus_tag="VYN80_000313"
     CDS             complement(42103..43152)
                     /locus_tag="VYN80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1540287.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(43271..45079)
                     /gene="glmS"
                     /locus_tag="VYN80_000314"
     CDS             complement(43271..45079)
                     /gene="glmS"
                     /locus_tag="VYN80_000314"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1540288.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYSMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(45271..46650)
                     /gene="bglA"
                     /locus_tag="VYN80_000315"
     CDS             complement(45271..46650)
                     /gene="bglA"
                     /locus_tag="VYN80_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1540289.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(46750..48603)
                     /locus_tag="VYN80_000316"
     CDS             complement(46750..48603)
                     /locus_tag="VYN80_000316"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1540290.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(48616..49875)
                     /gene="rseP"
                     /locus_tag="VYN80_000317"
     CDS             complement(48616..49875)
                     /gene="rseP"
                     /locus_tag="VYN80_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1540291.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(49897..50700)
                     /locus_tag="VYN80_000318"
     CDS             complement(49897..50700)
                     /locus_tag="VYN80_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1540292.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(50709..51467)
                     /locus_tag="VYN80_000319"
     CDS             complement(50709..51467)
                     /locus_tag="VYN80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1540293.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(51698..52162)
                     /locus_tag="VYN80_000320"
     CDS             complement(51698..52162)
                     /locus_tag="VYN80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1540294.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(52251..53222)
                     /gene="ruvB"
                     /locus_tag="VYN80_000321"
     CDS             complement(52251..53222)
                     /gene="ruvB"
                     /locus_tag="VYN80_000321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1540295.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            53383..53475
                     /locus_tag="VYN80_000322"
     CDS             53383..53475
                     /locus_tag="VYN80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1540296.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT        15728 a        12298 c        10141 g        15684 t
ORIGIN      
        1 gaatataatt tggttataga gaagtcttaa caaatttgaa aggaagtatc aaatgacaaa
       61 tacatcattc tcaattgagc agttttcttt aaaaggaaaa attgctctca tcaccggcgc
      121 ttcttatgga attggatttg ctattgccaa atcctacgct gaagccggcg ctactattgt
      181 ctttaacgat atcaatcaag atctggtcaa taaagggatt gaagcttatc gtgaagttgg
      241 catccaagcc catggatatg tctatgacgt gacagacgag gacggtatcc aagccatggt
      301 caagcaaatc gaacaagagg ttggtgtcat tgacatcctc gttaataacg ctggtattat
      361 ccgccgagtt ccaatgtgcg aaatgagcgc cgctgatttc cgtaaggtca tcgatattga
      421 cttaaacgca ccatttatcg tttcaaaggc agttattcct tctatgataa agaaagggca
      481 tggaaagatt atcaatattt gttcgatgat gagcgaactg ggacgtgaaa cagttagcgc
      541 ttatgctgct gctaaagggg gcttgaaaat gttgacccgc aacattgcgt ctgaatacgg
      601 tggagccaat atccaatgta acggaattgg accgggttat attgccactc ctcaaacagc
      661 acctcttcgt gagttgcaag aagatggttc tcgccaccca tttgaccagt tcatcattgc
      721 aaaaacacct gctgcacgtt ggggaaatcc tgaagatttg atgggccctg ctgtctttct
      781 cgctagtgat gccagcaatt ttgtcaatgg ccacatccta tatgtagatg gcggtatctt
      841 agcctacatc ggaaaacaac ctgagtaaaa atagaaagaa gatcttatga aaatcgcatt
      901 aatcaatgaa aatagtcaag ctagcaagaa tcacattatt tacgatagtc taaaagaagc
      961 gacagataaa aaaggctacc aattatttaa ctatggtatg cgtggagaag aaggagaaag
     1021 tcaattaact tatgtgcaga acggactaat ggctgccatc cttttaaata caaaggcagt
     1081 tgactttgtt gttaccggct gtggtacggg tgtaggggct atgcttgctt taaacagctt
     1141 ccctggtatt gtctgtggtc tagcagtgga cccaactgac gcttaccttt attctcaaat
     1201 caatggtggt aacgccttgt ctatccctta tgccaaagga tttggctggg gggcagaact
     1261 gaccctcaaa ttgatgtttg aacgcttatt tgctgaagaa atgggcggtg gctacccaag
     1321 agaacgtgta atccctgaac aacgcaacgc tcgtatctta aacgaggtga aacaaatcac
     1381 ccacaatgat ttgatgacca tccttaaaac aatcgaccaa gacttcctca aagacaccat
     1441 ctctggcaaa tacttccaag aatacttctt tgaaaactgc caagatgatg aagtcgctgc
     1501 ttatttgaaa gaagtattag ccaagtaaag ctattctaaa ccagaaagga actaatggat
     1561 gacgaaaata ttactgtttg gcgaaccatt aattcgaatc tcaccattag atgccaccag
     1621 tatcggcgat catgttgcca gttcgactta ttttggcgga tcagaaatta acatcgcttg
     1681 taatttgcaa gccctgggta tctcaacgaa agtctttacc gcactccctg ccaacgagat
     1741 tggagatcgt tttctcacat tcttgaaaca gcaccaaatc gataccagtt caatctgtcg
     1801 gcttggcgat cgaatcggcc tctactattt ggagaacggc tttggttgtc gtcaaagtga
     1861 agttttctac gatcgtaagc atacgagtat cagccagatt cggccaaaca tgctagatat
     1921 ggattctctc tttcagggaa ttagccattt tcattttagt ggaatcaccg tagctatcgg
     1981 tcaagaggtc cgtgcgatcc ttctcctact cttggaagaa gccaagcgcc gaggaattgt
     2041 cgtttcaatg gatctcaatc tgagaacaaa gatgatttca gtcctagaag ccaagtatga
     2101 attttctaag tttgcacgtt ttactgacta ttgcttcggt attgatcctc tcatgattga
     2161 tgaccaaaat ctagagatgt ttccaagaga cagtgctagc ctagaaaagg tggaaaatcg
     2221 catgcgactt ttaaaagaag cctatggttt caaggccatt ttccataccc tccgctctag
     2281 tgatgagcaa gacaaaaatg tctatcaagc ctatgctcta gaagaacaat ttgaagagtc
     2341 tgtccaacta aaaactgcag tctatcaacg aattggtagc ggggatgcct ttatatctgg
     2401 tgccctttac caactactcc atcattcctc cctaaaaact accattgact ttgcagttgc
     2461 gagcgcaact ctcaaatgca ctcttccagg agaccatctc tccacttcct caactagtat
     2521 tgaaaattta ctggcaaatg cacaagatat cattcgttag gagaattaca tgaccaaatc
     2581 agatacgatt attgaactaa aaaaacaaaa aattgtcgct gtcattcgag gaaatacaaa
     2641 ggaagaagga ctgcaagcct cgattgcttg tatcaagggc ggtatcaaag ctattgaaat
     2701 cgcctatacc aatcagtatg caggacaaat catcaaggaa cttgtagact tgtatcagga
     2761 cgatcagagt gtttgtatcg gtgcaggtac tgtgcttgat gccgtaactg ctagagatgc
     2821 cattctagct ggagcaaatt acgttgtttc tccatctttc catgctgaaa ctgcgaaaat
     2881 gtgcaatctc tacagcacac cgtacattcc aggctgtatt accctcacag agatcacgac
     2941 tgcacttgaa gccggtagtg aaatcatcaa actcttccca ggtagtactc tcagtccagc
     3001 atatatctct gcagttaagg caccgatccc acaagtttcc gtaatggtaa ccggaggagt
     3061 cggcctaaac aacatccctc aatggttcgc tgctggtgca gatgccgttg gaattggtgg
     3121 cgaactcaat aaactcgctt cccaaggcaa ctttgaccgc atcagcgaga ttgcccaaca
     3181 gtatgttaca ctcagataaa atcataacca cccgtcaaac gggtggttta tcccagagct
     3241 ataagcccaa atcatcagcc aagcgcctaa agacgctggc tttcacgttg ttcaaacctt
     3301 attgctcttg actcgtcact tgcctcttta agagactttg gtattactta ccactatccc
     3361 taaagggatc ctcatattct tttacactca atttatctag tgctatagta gattgaaact
     3421 ggaatagtac acatctactt ctaaaacatt gttaaaaatc gatttgactg tcctgatcga
     3481 tttgttctgt tcttatttca ttttactata tatcatgctt tactcgttct caaattttca
     3541 tactcgtgaa gaaatcatcc actggataat ttctttaatc ttgactatat ttcttaattg
     3601 tggctttatt aagccctact gtacttacat aataaccttc cgcccagaaa tgccgattcc
     3661 caaacttgta cttgagattg gcgtgtttgt caaacatcat gagtgcactt ttgcctttta
     3721 aataacccat aaaacttgaa acacttagtc ttagtggaat actgactaac atgtgtacat
     3781 ggtctggcat taagtgaccc tcgatcattt caacaccttt ataactacac aagcgatgaa
     3841 atatttcgcc taaactactt ctatattgat tatagatgac ttttcgtcta tacttagggg
     3901 tgaacacaat gtgatagaac acatccactt tgtgtatgat aaactatgag tcttttgtgc
     3961 catatttttt ctcctttcgc tttacaattg gattgaacac ctttattgta tcgcgtttgg
     4021 agtttttttg gtataatctt cgacgcgcac ccgcatagcg ggtggttgtt ttgtctcgca
     4081 cctcacggag cgagacggac taatatagtg gagcgaaata ggatacgaac aaattgatta
     4141 ggaaaatcaa atgaatttat agaaatcttt tagcagttat gacgtcctat tctagtttca
     4201 aaacgctata gtcacataat gaagtaaaaa agggataagt atcaacttat ccctttttaa
     4261 aagaaaaatc cgaagatatt tagccttctt cggatttttt ctattttcca cagtttcatg
     4321 taattcatct agatgatgaa caaattagtt gttctttcct ctacggaata aataaaacgc
     4381 cccaagtagc aagaacccta gacttgccaa gattgactga ccttctcctg tctgagggag
     4441 attcttttga tccgaatggt tcttttcctc ttcagatttt tccttttctt ttgaattctg
     4501 tacttgtggc tgagctgctt gctctagctt tttaaagact tcctgatctg gagctgattc
     4561 ctgagtttca ggattatagt aggcaatctt atattcatcc ccttcttttc gaatagtata
     4621 gactccacgt ttcaaaactt ggaattggtt ggaaatagta gagacagaat catcatattt
     4681 cacaatgccc caaactcctt gtttagcatc ataaacagac tgaagggttt cgttattttc
     4741 gatgaggctt ctttctaact cttttatcat ttgattgaag gtggcacgat ccacgttagg
     4801 aatgagcata tagccataag aatctccatt ttgcttatga tcctgactaa tcgtaagaaa
     4861 ttcattttca acttccttgt ctgactgtcc ttcattgata tccttccagg ctcccttttg
     4921 caaagcctta ctcatgctga ttgaactctt cttaaagaaa aagtaaccaa tattcttttt
     4981 cgaatcggac gattctaaaa agacactttg ggtttcagga taatcctttt cttgttctgt
     5041 aagggaggct tctttatcat tgacatagac tttatatgga ttacttgatt ccagttttct
     5101 ctggtcgatt gtagttgcag cagtatctgt tgaagtgttt tggatattgc ttcctaaaaa
     5161 ggcaatctta tcctttagca taaaccagct cttatgagca gtcaatgttt gattccagtt
     5221 ggtgaaatcc atggttgctg tcgcattggc atcatctagt ttgctcgttc caacgaaagc
     5281 agacggtaaa actttacctg tatcgctatc cgctctctta gcatccgtct ctgttgtacc
     5341 aggcatctta tatggattaa ctgttggcca gtagccatcg ctatagtgac tcaaatcgcc
     5401 attgtaaaga tagaacatcc catcactcgt ataccaacca cgtttatttt ccttgttcat
     5461 gtgttcgtaa ttcaaggtac gactggaaaa gagtgacaag ccaaatccaa accctttctc
     5521 tgcattgtac atggctgttt tatccatctt gttaaaggca gataggtaac ttgttcttgg
     5581 aacacttgcg actcctgcat cacttaacaa ggattgcatc aaactgatat ccttataagt
     5641 cttcaaattc ttaaagacat cataataact atccgattga acaatggtct tcacaagact
     5701 ctgcaaacgt tgtttggttt ctccttcaga catatccgct attcggtgaa tccctcttag
     5761 tacttctact gcggccacgt gcccctcgct atttgcacga ctgatcgagc gtccacgact
     5821 catatccatc agctctccat tcaccagcaa aggagcaaac gatttatcaa tccagtggta
     5881 catggtttgc attttatctt tatcgattgg attcttggtc ttttgaatga ctggcaacag
     5941 ttgagacagg ccatcaatca aaacattccc ataagcaccc gtataggcaa cattggtgtg
     6001 gtcgatatag gatccatctt gataaaaacc ttcaccttgg tctaccaact tgaacacttg
     6061 ctcaatcgag cgaatggtag aagaaatttc ttgatcatcc ttacgcagta aaccagctat
     6121 tacttttact cttcccatat caactaagtt tccacctaga gccttaaatg ggttatcagt
     6181 cgtctttcgg aaatgttcgg gatctggtac aaatttttca atcacatctg tatatttttt
     6241 aatttcctca tcagagaagt attctttcat cagagacaag gtattgttga tggcacgagg
     6301 tgtaccgatt tcataatccc accagttccc aacaatgctc ttttcacgat tgtagacatg
     6361 tttatgcatc cattccatgg aatccctgac tgttcgaacg acagtttcat cttgataata
     6421 acgagaagaa ggattggtca cttgcttggc catctcctcc aatttccgat aagtggcagt
     6481 cagatttgca gacattttat aatttgaaaa tttttcccac aaataggtgc ggtccgcctg
     6541 acttgaaata ctagataggc tatcagctac ctttccttcc aattcctggt ttaatttggc
     6601 catctgttca tttttagaat catagtattg attcccagcg atgatgccat tccagtcatc
     6661 caaacgggct gtgtatgcat ccttaacaga ggctagaatc ttcaaaggaa tctttttcac
     6721 ttccttgcca tctttactga caatgacatt ggttgtccct tccttaagag gttctaaaat
     6781 tccatttttg actgaagcaa cgtcaggatt ttctacctta taagtatagt ccgcaagaga
     6841 aaaaacatgt ttttttccaa ttggtaaatc aatcttttcc tcaagctgtt tatctgtttg
     6901 agaatcctca gaaagctggg ctgctacctc taccagctca atatccttaa aggaaacagt
     6961 cccagttcct gtttcataga ataactccag cttgatttta tcaacatcta aagtcgggct
     7021 atagtctgct tcaatggtct gccagtcctt tgttcctgac gtcgttgcag aattccacaa
     7081 tcgcttgtcc ttaccacttt cctcaatgat acgaaccttg gcaatcccga ctttattatc
     7141 tgttttaatc ttgaaacgca gtttatactt tttcttagct tcaataggaa ccatacggtg
     7201 aaccgctgcc cttaatttct cagggcttga gatagtgata gccccatcct tagcctcaat
     7261 gactcgagtt gaggcatctg cagaactatt cttctggtct acccaagctg accacccctg
     7321 agctttgctt cctgtccacg gactgcttcc gtcctccgtc tgaccaaaat caccattttc
     7381 aactaaattt tttgtatccc tttgctgata ctgaacttcc ttctggcttt gctgaatggt
     7441 attcgtagtc gtttcttccg ctccaactgt catataatgg tttaataaaa atcctgataa
     7501 aacaagtgaa gacaaactca caatgagctt ctttgttttt gtttgcatgt atacactcca
     7561 ataaacatat tgtaaaatca ataggaacta aatctcaaat tagagattta tagcaacaaa
     7621 gtaaaccggt ttaattaaat cataccattt ttaaagttat ttgtaaacgg tttctatcat
     7681 attgagggct tattcttcaa caatatcttc catccgactc gacgaaaaaa gtaaaaactg
     7741 ttacaatgta gaaaaagaaa agaggccctc atgacttcac tttatgattt ttccgttttg
     7801 aaccaaaaca accaagcaac tcccttggat agctatcgtg gcaaagttct cttgattgtc
     7861 aacactgcta ctggatgtgg tttaacgccc cagtaccaga gacttcaaga actctatgaa
     7921 cgctatcaag atcagggctt cgaaatattg gatttccctt gcaatcagtt tatgggacaa
     7981 gcacccggca gcgcagagga aatcaacacc ttctgtagcc tacattttca aaccaccttc
     8041 ccacgttttg ccaagattaa ggtcaacggt aaggaagcag accctctcta tgtctggtta
     8101 aaagaccata aatctggccc actaggaaaa cgaatcgaat ggaatttcgc taagtttctc
     8161 attggtcgtg atgggcaagt ctttgaacgc ttttcttcaa aaacagaccc aaaacaaatt
     8221 gaagaggcga tacaaactct actataattc acaatctcac tatgattagg tttcctttaa
     8281 cctgatgaat agtgagattt tttgattggc tttgacttaa atagaaaaac accccatgat
     8341 atgaaacatg aagtgttgta aagtctatgt tgtaggtgct tatttcacat tttcaatgtg
     8401 accagtgata acgaatacca tacagaatct tcatatacac taaacaaatg actttctaat
     8461 tatttcaatt agttttggct agtaaatatc atttccaaca aacgccctct caattcctta
     8521 tcctgatgat gcaagatatt cattaagtca tgagagtttt tcgcattgat gaattgattt
     8581 aacaatctat cttttaattc atatggaaga gaagctgtct ttagtagtct aaaaacttcg
     8641 tcatttaaag atgtcctttt attatctttc cattcaaatt tagctgtatc attcttattt
     8701 ggcaattcaa ttatagacac attcgttcct ttaaaatgaa ttctatgttt tctattgctt
     8761 ggaacgatac tagaatctcc ttgtaatgct aactctacca ttcccatttc ccaatcgatt
     8821 gataatcttg ttttatatct ttgaccattt tgatcttcaa gcatttcaaa agaatgttgt
     8881 ttccctggga atacatacca atctacaacc tcaggtaaat caacacccat acctatctca
     8941 gaaccaacca agggaatgat tgcaccactt tttgcaaaca caggcgtagt cgagatgtcc
     9001 ctataaacac ttaacttcac accacctgtg tattttttct ctgaaaagaa gtcataccat
     9061 tcaccttcag ggaaccatac atctactttt gcagattgga atgtcaaatc catcttttct
     9121 acaatgggag ccaccatcag ttctgttcca aaaaagtatt ggtttggaac attatagctc
     9181 tcatcattct ctggatagaa ataatagatt ggactgatta atggggcacc ttcctcatgt
     9241 gtctttacat tcatggtata tagataagga atcatctgat gtctcaaacg aaggtatttc
     9301 ttcataatct tagatgttgt ttctgaaaaa aaccaaggtt ctttactatt aaaaggactt
     9361 ctagaactat gtaatcgagt aatcggacta aaaacaccaa actgtagcca tctagtttgt
     9421 agctcttcgt cataatcccc caacatatgt ccaccgatat catgactcca ccaactataa
     9481 ccgatattag atgctgtcgc tgtaaaatag ggttgaaatc ttaaggaatt ccaactgata
     9541 atagtatccc ctgaaaaacc aacagggtag cggtgactac caggacctgc atatcttgat
     9601 aaaatcaaac caccttctgc atttttacaa ctatcctgat agtgataatg gtttaaaagc
     9661 caaagtggat ctagcatgcc ttgtgtcccc tgttgccagt caatccacca aaaatctact
     9721 ccctgctttt ctagctcata atgaacatct ttaaagtagg cttccctaaa agagggatta
     9781 aaaaaatcaa aaatagcagg ttcttctagt tctacattta accccaaccg ttttgcgatt
     9841 tgaggataag cttcttcata agcccgtatc ccatcagcag gatggacatt taaggagagt
     9901 tttaactttc tatcatgaag ttgttgcaat aactgttctg gatttggtat taagtttcta
     9961 ttccaactat atcctgtcca gccacttcca aaacgagctg gaatgtcagt tatatgccaa
    10021 tccatatcta acacaccgat agataatgga attttctctg ttgcaaatct gtctattaaa
    10081 tccaagtatt catccgacgt ataaggccaa tatctactcc accaattgcc taaagcatat
    10141 cttggcaaca agggtgttga accagtcaaa tggtaaaagt ctctgattgc tcctctataa
    10201 tcatgcccat aggcaaagaa atacaggtca atttgatttt ctctctcaat ataaccagat
    10261 tgttcatccc aaataaatcc ttgagaatca tccaataagg ctataccatt tcggctaata
    10321 attccatctt ctaacgagat tgctccatct gccttatcca gagtccgagc tgttcctttt
    10381 aacgtttcaa tagattcacc aaaataccag cgactaccat atacggcaaa atttcctttt
    10441 aattctataa ataaattttc ggcgttaaat tctcctttat taaagtgcag atgaaaatag
    10501 tccgtcataa tatctagtac gtttgatgtc tcgatataat ctaacgaaac ttggccaaaa
    10561 tctctattat agataagttg tgtcgttcta tcctcaaaac ttccagtttg agagtattct
    10621 aaccttacta gcttgtctgt taatacagag attcgataaa actctccctt aaaaattttc
    10681 aatttgtttt cctcctttta tggtagcata aaaacagaac gcaccatttt tgatgcgttt
    10741 ttcattattc tgaatgcaat gttctatctg ttatatctat gacaaataat agtcaattga
    10801 aaaaatgtag tggacaaaat atcttttaac aaaccaagag tttattaaag agttatcact
    10861 tttcaacttt tctaagctta tgcagttgtg aaacaaacta cttttaaact attaactaag
    10921 ataggattga taaataattt caaactctta ctagcaatca tacgatattc aagctcgcgt
    10981 gcttttttcc ttcctgctta tttcttagaa ctgaagaacc cggatcggta tataaattat
    11041 ccggatcaac atagtcataa gactcataac agttgcgctt cattaagtca tccccagagc
    11101 aagagcttca tctcgtaatt tttcaacatc actaaccgta ggtcgccatc cttcaatcat
    11161 atttgtactt aaagcatacc aaacactctt aaaaacggat cggttttcaa aagctattcc
    11221 catgattgtc atcttttctt tatctatatc taaggacata tgctacctcc tttagataca
    11281 ttataccatg tttctctgta gcttttaaaa attttatttt gtttgtcata tctaagtttt
    11341 cagtacgctt atcctatttt ataagcctca aacccaaata taaaacgcat taaccatctg
    11401 ataatatgcg ttttattgtg gaaacatttc ccattttctc tatttctaat gacttacttc
    11461 aacttctgac ttcatggaaa aaacaacaaa aatatgaact ggcaaagttt ggtatcatca
    11521 gtaatccaga acaattcatc tttacttaca tagataccaa aggcaatatc aataaacctt
    11581 tacattctga ctatctcaat aataagatga aaactatcag aaaacgacac ccagagctta
    11641 cacacgctac gccacacaaa ctacgtcata ccggagcaac gctcgctaaa caagcaggaa
    11701 tgagcttaga agccatttct gaagctctaa tacatagcga cacaggtaca acacagattt
    11761 atgtcaatac ttctaatgta gtccctatgg ccgtcggtga atttgcctta cagtctctaa
    11821 aacaataagg tgaaaataag gtaaactttg aggtgaactt tttcaaaaaa cataaaaaaa
    11881 gcacccatga ggtaaactct tgagtgcttt gaaacgttga tataattgta ttgattaacg
    11941 ttttgaggca actgaccttg tcaggttgca gccacatttg taagcgacta aagtcgcaac
    12001 aactgtgtca attgcaccaa tttagtaaga aagtataaaa aaagaacacc ccgaaaggtg
    12061 ctctgtataa gtatagtaat tctttcgaat taacgtttac taaattgtga tgctttacga
    12121 gctttcttaa gacctggttt cttacgttca actttacgtg agtcacgtgt aagaagtcct
    12181 gcgcgtttca atgaatcgcg gaagtctggg tctacttgaa gaagggcacg agcgataccg
    12241 tgacggatag ctcctgattg accagcgtat ccaccaccta caacgttaac gaaaacgtcg
    12301 tatgaaccta cagttgaagt aactgcgaat ggttggttga tgacaagacg aaggtcagcg
    12361 tgtgggatgt actcttcaac atcttttttg ttaacagtga ttttaccagt tcctggaaca
    12421 aggcgaacgc gtgcaacagc gtttttacga cgtccagtac ctgcatattg tgcttgtgac
    12481 atactttatt tgttcctttc cttagataag tcctgaaatg tcaagaactt ctggttgttg
    12541 tgcagcatga gtgtgctcag ctccaacaaa tactttcaac ttcatacctt gagcgcgtcc
    12601 aagtgtattg tgtggaagca tacctttaac tgatttctca atcaaacgta ctgcattttt
    12661 agaacgaagt tcacctgcag agatttgttt caatccacct gggtggtttg agtgagtgta
    12721 gtagatttta tcagttgctt ttttaccagt caatttaact ttttcagcat tgataacaat
    12781 cacgaagtca cctgtatcag tgtgtggtgt aaatgttggt ttgttttttc cgcgaagtac
    12841 gctagcaact actgcagaaa gacgtccaag tggtacatca gttgcgtcaa ctacgtacca
    12901 tttacgttca acttggcctg gtttagccat aaatgttgtt ttgttcatga tttctcctat
    12961 atatagttcg atttttgttt acggtgatgg gtgttccttc ccatcgaaaa gtatttggaa
    13021 ggttccgggg cctttcaaat ggggtaaaca ataccgccta ctatcatacc aaatttcacc
    13081 cctaaaagtc aatagatatg aaaaatattt ttcggtaact tctcaaagta actgaaagtt
    13141 atataatcaa gttaatttgg gaatttacct attaacttcg gtagattttt gggaattttt
    13201 ttccttcttt ttccctgtag ttaaaatcct tttcaaaaaa tcaccttttc ttgattttta
    13261 atcctatttg ctgtatgata agggaaaaga aaggagacag agatatggct tttaccaata
    13321 cccacatgcg gtctgctagt tttggcattg ttaccagctt gcctgatgac atcattgact
    13381 ctttttggta tattatcgac catttcttaa aaaatgtctt tgaattggaa gaagaactcg
    13441 agtttcaatt gcttaataac caaggaaaga ttaccttcca cttttcaagt caacacctcc
    13501 ctacagccat tgattttgac tttaaccatc ctttcgaccc tcgttatccc ccaagagtac
    13561 tggttttaga catggacggt agagaaacta tcctcctccc agaagaaaat gacctatttt
    13621 aaaaactcta gccttcagtt gcaagtaact gaaaactaga gtttttctat tttttcaaag
    13681 catcatacaa gttacggatc ggttgtttta atatcggatg gataaaatga ggcgcaattt
    13741 cctgtaagga ctcaaggaca aaaaggcgtt ccgctatgta aggatgaggc aatatgaggt
    13801 cgtctgtata aaggatctgg tcctccacaa agagcaagtc caaatcaatc aaacgaggtc
    13861 cccaatgcac ttctctcacc cgtcccagct ctgactcaat ggctaacaag gtttctaaca
    13921 agtcttgtgc tggtagccag gtttccacct caaccacttg attggcaaag ctatcctgct
    13981 ccactccacc ccaaggctcc gtcgctaaga cactggactc tttgagaata tagatgccac
    14041 gagctcgcag tttgtcaatg gcttgcttca agtttgcttg tttatctccc atattgcttc
    14101 ctagggcgat aaaggctcgt tgcttgcggc gatgaatggt taccgagcaa gtatctagtg
    14161 acaaatgcac cggtgcccaa ggttttttca gttccaactt catttcttgg acaagaggat
    14221 aagactcaaa ggtacgttcc accagtttat aggctaccgt ttcaatcaaa tcttcactcg
    14281 tttcctgaaa ccaagtcgtc cactgctgac acaattctcc gtaatggaca gaggctgtta
    14341 aatccaagtc tgtagctgcc ttggtcatat cataggatag gatggctgaa acgacaaatt
    14401 tctgccccaa ttctttctca ctaggaaaaa gaccatgata ggcaaacatt tccaaatcct
    14461 taatctgcag ttgatccata atgattcctt tctagaaaaa tccgcttgaa acggattctt
    14521 tttatagccc cattaaacga taagcttggt cgcggaggtc cttatctgtt tcaaatagac
    14581 cacgagctac tgtcgtcaag gttgcagtgc ctggttttct aacaccacgc atactcatac
    14641 acatatgttc cgcctcaatg acaacaaagg ctcctttagc acctagatag tccatcaagg
    14701 catcggccac ttcgatattc aaacgttctt gaatttgtgg ttttttcgaa taaacttcaa
    14761 ccgtacgggc tagcttagac aagcctgcca cacgaccatc tggaatgtag gcaatgtgcg
    14821 ctctaccata aaatggcaag aagtggtgtt cacacatggt atggaaaaag atatcctttt
    14881 ctaccaccat attatcgtca ataatttcaa aggattttga caaatgttcc tctgctgttt
    14941 gaccaagacc tgaaaaaatc tcttgataca tacgggctac acgagcaggt gtttcctgca
    15001 agccctcgcg attagcgttc tctcctacag cctcgataat catttttaca gctgcttcaa
    15061 tcttttgtgt atccattctc gttcttcctc tccatcagat aggccctcac ttggctcaag
    15121 aaatacaagg aacctgtgac aatcctaact gtttgtttct cttctttttt atctgtcaaa
    15181 ttctgctcta gaaaatcatg ccaatcttgg tagctgagat ttctagactt agctgcctct
    15241 ttcagcacgc tttcatccgt cgcccgacta tccgcaaaat gtgttagagt aagctcggta
    15301 tctggcatag ctcccagcaa gtccaacata tcccccaagg ctttggtttt gatacaagtg
    15361 aagaggattt ccttatgata atccgcaaaa cgttcttgca aggttaccaa caaggccttg
    15421 atagcatggg gattatgggc tccatccaaa atcatcaagg gatctcttga cacgatttcc
    15481 aaacgccctg gccaacttgt ttcttccaag gcttgaccaa gaaagtcatt gcttgctagc
    15541 tctcgaccat cttcttgaca aaaagtatca agtaaagcta tggccatccc agcattctct
    15601 atttggtaca aaccaagcag gctagtctgg aagcgacctt gtctgacagc acttgtatag
    15661 tcaaagactt cccctgtcac cacactttct tgatgacgaa cctgataatc tgtcccgtag
    15721 gcaagtctcg gcgcatcttt cccttccgca atgcggtcaa tcacagtcaa ggcttctgga
    15781 gcaatacgcc ctgttaccaa gggcatccct tgtttgataa tacctgcctt ctgctctgct
    15841 atggcctcca aggtgtcacc aagtagggcc acatgatcca agccaatagt tgtaattcct
    15901 gtcaaaatgg gctgacagac attggtacta tccaaaagtc cacccatgcc aacttccatg
    15961 atagccacat ctacttgctc tgaggcaaag tagtcatagg ctagggctgt gataatctca
    16021 aactcggttg tgccctgtaa attggcgacc gcttctccct ccagcaaaga ctgatagtct
    16081 gccatgagag cttctagcct cgcttctgag atcgattccc cattgatgct aatctggtct
    16141 gtgtaatgaa tgagataggg cgaactaaac acgccaactc tcagccctag cttttctagc
    16201 atctttttca aaaaagcaat agtcgagccc ttgccgttag tccctccgat atggagaacc
    16261 ttgagtttaa gatggggatt gccacgcaaa gctaacagtt ccaccattcg ttccaagcca
    16321 aaatgcggtt gatccgtccg gtagttagca atccactgat tgttttcaat ttctttcatc
    16381 ttatttatat tgttttaaat ctaaattttc cgcttcatca gccagacgaa tggcagaggc
    16441 aatttcaact gccatcctgt gactagctac gtcatgcacg cgcaccactt ctacaccctg
    16501 tctcgcagcg atactagtta catgagccga agccgtgtcc cgatttcgga aaccaagctc
    16561 tgtctcagga ttgacttcaa acccactttc ttcaaggata ttgatgacaa atcgcttgcg
    16621 cgacactccg agaaagattg gatagccctt ctgatgtagt ttatccaggt cccgtaaaag
    16681 gagaagattt tctttcttgg tcagaccaaa gccaattcct ggatccaaca ggatattttc
    16741 tggtgcaata ccagcttctg ccgctctcgc tagtgctcgt tcaaagaaag ccaccatcaa
    16801 gtcttcgatt ggcaatgttt caaagtcagc taactctttt tctgtaaagg tttgaccaaa
    16861 gccaaaatga gggaagataa gcgaactagg atgctgaggt cgagccataa ctgggttaaa
    16921 catgatgacc actttcgctc cagctttagc aaccacatgg gccatttttt catcacccat
    16981 gagaccagtg atatcattga ctagatcggc accagcagcc aaagcagcct ctgctacttg
    17041 actcttccaa gtatcaatag agatgaggac atcactttcc ttgcgaatcg ctttgatcac
    17101 tggaacaaca cgctggattt cctcttctat ctcaacatag ctacttcccg gccgagtcga
    17161 ttctccgccg atatcgagca tactggctcc ttctgctatc aatttacgag cctgctggag
    17221 tgcctgctca agagcaaaaa attgaccacc gtccgaaaag gagtctgggg ttacattgat
    17281 aattccgcaa ataactgtct ttgcatgatt ggctttactt gacatatcgg tcactccctc
    17341 aaggattttc atcatattat ttttctattt taccataaaa agaaaaagat ggacacgatt
    17401 gcattcatct ttccctagta gaaagaggtc ggttcctaca taaaaacttt taaacagaaa
    17461 ttcctaatgt ccgactcata atcaccacaa gagccaacaa acagaaagca atcccattaa
    17521 caatcatgtg aagcaagatc gacatttcca aacgttgggt cttgtaggcc gtccaagata
    17581 gaactgtcga catacctcca taaatcaata aagaaggtaa attacttggt tgatgcaata
    17641 aagcaaacac aatcgtaccg actacaaatc ccaagttctc cttgcctcgg aaaatctttt
    17701 taggaacaag cccacggcac aagatttcct cacaaattgg agcaagcaag gctagcaaga
    17761 agaagctaga aatcagagaa ctattctgaa ccatatcgtt aatctgagac tggttagctg
    17821 ttgtcgtctc atttgacagt tgcaacaaga tagaaccaag tatatttgaa cagataatga
    17881 ctaaataact caagcccaaa cgtgccaaat ccttagttct aaaaaaagaa aaattaaaac
    17941 ttgccaattg cgttttacga gctccaataa taaatagcac cagaaccaca atagaaatac
    18001 cagtaactat cagccctgac tgtagcaggg ctacttcttt taaagtcaaa atagaggtaa
    18061 ccactagggg aacctgatat aaaaggacgg caagtaaaaa gaccaaaatc cagcctgcac
    18121 gattcaacaa ttctttccac atatttttct ctttcatcac ctatctcctt tgtattaaaa
    18181 aagggggaaa atccccctgg gtcattctat tataaggcca tgctgatgta gttaaggata
    18241 aacaaggcat ccaaaatcca aatcatggca tgaacatctt taacttgacc tttgacaagc
    18301 ttagtcaaag tgtaagtcaa gaaaccaact gcaatccctt gagtgataga gtagctgaat
    18361 cccataaaga tagatgtgaa gaaggcagga actgcttcag acatatcatc ccaatagata
    18421 tttttcaagc taccaagcat cataatccca acgataatca agattggagc tgtagccgct
    18481 gttggtacga tcgctagaag tgggctaaag aagcttgaaa tcgcaaaaca gatagctaca
    18541 accaaggctg tcaaaccagt acgtccacct gcaccgatac cagcagcaga ctcaacataa
    18601 gtcgttacgt ttgaagtacc tgcaatggca ccgactgtcg ttccaatcaa atcagagtaa
    18661 agagccttat ccaatttggc tgattggtga ttttcaccat ttgtcgctac aataccaact
    18721 ttttcacctg taccgatcaa ggtaccaatt gtgtcaaaaa tatctgtcaa tgagaaggca
    18781 agaatggcca tcagagtttc aggcaagcga gctgtatctg aaaccaaagc tcccaaacct
    18841 tctgaaccaa gagctgcacc aaagattgtc ttcaaatctt caaaagctgc accaacatgg
    18901 ttattagcaa aatcgatact agacaaatca accaaaccaa ctgcaatagc aagaacagtt
    18961 gttgtcaaga tagagagaat aattccccct ttaatccctt tgatgacaaa gaagatagta
    19021 atggcaagtc ctgcaagagc cactaaaaca gctggattat taaagctgac caatcctgga
    19081 actgctgaag agtttgctgc aatcgttgct tgagctttgt cagccccttc tcctacaaca
    19141 gtatagttgc ctggatcaat cgtgaatttc aaaagtccag cattcttaat ccctacatag
    19201 gcaaggaaga caccgatacc agctgaaata gctgagcgaa gagcattggg aatcgattca
    19261 atgatcattt tacgaacatt tgtcaaggta ataatcaatg aaataatccc acagatgaag
    19321 accatagcta gggcttcttg ccaagaataa ccaagcccga atacaactgt aaaggtaaag
    19381 aaggcattga gtcccatacc tggcgcttgg gcataaggta agttagcata aaaagccatc
    19441 atcaaggtac ccgctactgc accaataatc gtcgctagga agacgccctg agcaggcatt
    19501 cctgtttgtg aaagtatttg tgggtttaca aagagaatat agctcattgc aaagaaagtt
    19561 gttaaaccag cgagaacctc tgtacgaacg tctgtaccgt tctcttttag tttaaataat
    19621 ttgtccatca tcaatctcct tttaattttt acgaacaata atagaattat atcatttatc
    19681 attcactttt tcaatgtctt tgtttgattt gtttaagaaa ttaatccaac ttcatttttt
    19741 gtttggaatc tgcttttctg cctgctttct gttataatag aagacatctt atctgaaaac
    19801 acactagaaa ggtccatatg acgaaaaaaa ttattgcagt tgacctggat ggaaccctgc
    19861 tcaactcaga cagtcaaatt tctgacttta ccaaaaaaac catcaaaaaa gttgctgaaa
    19921 aaggccatca ggttattatt acgacaggtc gcccttaccg tatgtcaaaa gatttttacc
    19981 gtgaactggg cttagacact cctatgatta acttcaacgg atcccttact catttaccag
    20041 accaagtttg ggattttgaa aagtgtttga ctgtagacaa aaaatatctg ctagatatgg
    20101 ttcaacgttc agaggacatt caagccgatt ttatcgctgg agaatatcgt aaaaaattct
    20161 acattacaaa tcccaatgaa gagattgcca atcccaaact atttggtgta gaagctttcc
    20221 agcctgaaga tcaattccag cctgaattgg tgaccaagga ccctaactgt atcctcttgc
    20281 agaccagagc cagtgacaaa tatgccttgg caaaagacat gaacgccttc taccagcatc
    20341 aactttctat caatacctgg ggaggtccgc tcaatatcct tgaatgtacc ccaaaaggtg
    20401 tcaacaaggc ctttgctttg gactacttgc tcaagataat gaatcgtgac aaaaaagatt
    20461 tgattgcctt tggagatgaa cacaatgata ccgaaatgct cgcttttgct gggaaaggtt
    20521 atgccatgaa aaatgccaat ccagagctac tcccttatgc agatgagcaa atttccctta
    20581 ccaacgacca agatggggtt gccaaaaccc tacaagactt attcttatag cctacactga
    20641 tactcaatga gaatcaaaga gcaaactagg aagctagccg taggttgctc aaagcactgc
    20701 tttgaggttg tagatgaagc tgacgtggtt tgaagagatt ttcgaagagt atgaccagct
    20761 tgcaagctgg tctttgtgct cttttcacag tctcatcaac cagttaatcg attgttctac
    20821 tttttgtctc caaaaccttg gaaaaggctt tcttttgtga tatacttcac atataaatac
    20881 aagagagctc gtcacactcg actctgttga caaaaaatca atccagtccc gttgtccctg
    20941 ttctcggtca atatcaagga ggatagctat gaaagctgtt gttgtaaatc cagaaagcac
    21001 tggtgttgct attgaagaaa aagtactccg tccacttgaa actggggaag cacttgtaga
    21061 agttgaatac tgtggcgttt gccacaccga cctccacgtt gctcatggtg actttggtca
    21121 agtcccagga cgtgttcttg ggcacgaagg tatcggtatc gttaaagaga ttgctccaga
    21181 tgtgaaaagc cttaaagtcg gtgaccgcgt cagcgttgct tggttctttg aaggatgtgg
    21241 cacttgcgaa tactgtacaa ctggtcgcga aaccctttgc cgtacagtga aaaatgctgg
    21301 ctactcagta gacggtggta tggctgaaca atgtatcgta actgctgact atgctgtcaa
    21361 agttcctgac ggacttgatc cagcccaagc ttcttctatc acatgtgctg gagtaacaac
    21421 ctataaagct atcaaagaag caaaagttga accaggccaa tgggttgttc tttacggtgc
    21481 tggtggactt ggtaacctcg ctgttcaata cgctaaaaaa gtattcaatg ctcatgttat
    21541 cgcagtcgat atcaacaatg acaaacttac ccttgcaaaa gaagtaggcg ctgacattgt
    21601 gattaacggc ctcgaagttg aagatgtagc tggactcatt aaagaaaaaa ctgatggagg
    21661 agctcattca gctgtcgtaa ctgctgtgtc taaagttgcc ttcaaccagg ctgttgactc
    21721 cattcgtgct ggtggtcgcg tcgtcgctgt tggtcttcct tctgaaatga tggaactcag
    21781 catcgttaaa acagtcctcg atggaatcca agtcatcggt tctcttgtcg gaactcgtaa
    21841 agacttagaa gaagccttcc aatttggtgc agaaggtctg gtagtcccag ttgttcaaaa
    21901 acgtccagta gaagatgctt tttccatttt cgacgaaatg gaaaaaggcc aaatccaagg
    21961 acgtatggta ctcgacttca cccactaatt taatagaaac ctattttaaa agttggaagc
    22021 ttcttccaac ttttttatat taaattttaa ataagaattc aagattactc ctcaaaaact
    22081 caacatatca acatacaaaa ataaaaattt tttaattttt tatgataaat agttgctttt
    22141 tttatgaaaa cggtttatac ttaaacagtc ttaaagtttt cttaaatgaa aactaaaaca
    22201 aaaaaggagg aatgacaata atgagtatcg gaatcattat tgcgagccac ggcgaatttg
    22261 ctgcgggtat tcatcagtca ggatctatga tctttggtga acaagaaaag gttcaagttg
    22321 tgacctttat gccaaatgaa ggtcctgatg atctatacgc taagtttaat aacgctgttg
    22381 ctgcatttga cgcagaagat gaggttctag ttttggctga cctttggagt ggttctccat
    22441 ttaaccaagc tagtcgcgtg atgggagaaa atcctgagcg taagtttgcc atcatcacag
    22501 gacttaactt accgatgttg attcaagcct acacagagcg cctcatggac gctgctgcag
    22561 gtgtagaaaa agtcgctgct aatatcatta aagaagccaa agatggcatc aaagctcttc
    22621 cagaagagct aaatccagtt gaagaagttg caagcgctgc agctgctcca gttgcccaaa
    22681 ctgctatccc agaaggaact gttatcggag acggtaaatt gaaaatcaat cttgcccgtc
    22741 ttgacacacg tctacttcac ggtcaggttg caactgcttg gactccagat tcaaaagcaa
    22801 atcgtatcat cgttgcttca gataacgtgg ctaaagacga ccttcgtaaa gaattgatta
    22861 aacaagcagc tccaggtaat gtcaaggcta acgtggttcc aattcaaaaa ctgattgaga
    22921 tttcaaaaga cccacgtttt ggagaaacac atgcccttat cttgtttgaa acacctcaag
    22981 atgcccttcg tgccatcgaa ggcggcgtgc caatcaagac tcttaacgtt ggttctatgg
    23041 ctcactcaac aggtaaaaca ttggtcaata ccgttttgtc tatggacaaa gaagatgttg
    23101 ctacatttga aaaaatgcgt gacttgggtg ttgaatttga tgtccgtaaa gtaccaaatg
    23161 attctaaaaa agatttgttt gacttgatta acaaagccaa tgtcaaataa gccattattt
    23221 atgaaaggat tttaaacatg tctattattt ctatggtttt agtagtcgtt gtagccttct
    23281 ttgcaggtct tgaaggcatc ctcgaccagt tccaatttca ccaaccactt gtagcctgta
    23341 cccttattgg gcttgtaaca ggtcacttgg aagcagggat tatcctcggt ggatcgcttc
    23401 aaatgattgc ccttggttgg tcaaatatcg gtgctgctat cgctcctgat gctgcacttg
    23461 cttctgtcgc tgctgccatt atcatggttc ttggtggtga ctttaccaag actggtatcg
    23521 gtgttgccca agcggttgct atccctcttg ccgtagctgg acttttcttg acaatgattg
    23581 ttcgtacaat ttcagttggt ttggttcata ctgcagatgc tgccgctaaa aaaggtgact
    23641 tcggcgctgt ggagcgtgcg catttcattg cgctactctt ccaaggactt cgtatcgcgc
    23701 ttcctgcagc tcttctcctt atggtaccaa ctgaaactgt acaaagtatc cttagtgcca
    23761 tgccagactg gctcaaagat ggtatggcta tcggtggtgg tatggtcgtt gccgttggtt
    23821 acgccatggt tatcaacatg atggcaactc gtgaagtatg gccattcttc gctcttggtt
    23881 tcgttctcgc tgctgtgtca gatattactc taatcggatt cggtgctatc ggtgttgcta
    23941 tcgctcttat ctaccttcac ctttctaaaa ctggtggaaa tggtggcgga ggagccgcaa
    24001 cttctaacga cccaatcggc gatatcctag aagactacta agataagaaa ggactgaaaa
    24061 catcatgact gaaaaacttc aattaactaa atcagatcgt aaaaaagttt ggtggcgttc
    24121 aaccttctta caagggtctt ggaactttga acggatgcaa aacttgggct gggcttatac
    24181 actcattcca gctatcaaaa aactctatac taaaaaagaa gatcaaatcg ctgctcttga
    24241 gcgtcacctt gagttcttca acactcatcc atacgtagct gctccagtca tgggggttac
    24301 tcttgcgctt gaagaagaac gtgctaacgg tgtggaaatc gatgacgctg ctatccaagg
    24361 ggttaaaatc ggtatgatgg gacctcttgc tggtatcggt gacccagtat tctggtttac
    24421 agtacgccca atccttggat ctctcggtgc ttcacttgcc cttactggca atatcttggg
    24481 gccactcctc ttctttgttg catggaactt gattcgtatg tcattcttgt ggtatgttca
    24541 agagattgga tacaaggctg gatcagaaat cactaaagat atgtctggtg gtatccttca
    24601 agatatcact aaaggagctt ctatccttgg gatgttcatt cttgctgtcc ttgttcaacg
    24661 ctgggtaaat attaaatttg ctttcgatgt ttctaaagtt caactagatg aaaaggctta
    24721 tatccattgg gataaattgc cagaagggtc taaaggtatc caagaagcat tcgcacaagt
    24781 aggacaagga ttgtctcaaa ctcctgaaaa agttactact ttccaacaaa acttggatat
    24841 gttgattcct ggattatcag gactactcct tactttactt tgcatgtact tacttaagaa
    24901 aaaagtatct ccaatcacta ttatccttgc cctcttcgca gtgggtattg tggcacatgt
    24961 tcttcacatc atgtaatcaa gcaactaaaa aggaaccagg ttctaaaatc tgattccttt
    25021 tttctatgct tttatttagc caaggctccc attggatccc atggtgcaag tacaattggt
    25081 tctgctccat aggcagcttg ttcttctgct gtcagcaatt ccttacgaac aacgatttgg
    25141 tatgtgtatt cgtccatcca agcgtctgag gcaacaaagt aaccatctgt accgaccttg
    25201 tctccccatg agttttcaac cttccacttg gttgatttac cattttcgtc caagtcaaca
    25261 cctgtcaaga ccatggcgtg ggtcatcaag ctttcgctat agtccaaacg tccagccttg
    25321 tcttgagtaa gtttaatgtc catgcttgat tcaaagtcat aaacatctgt cgcaaggatt
    25381 ccagccttac ggttactgag ctggccgaca tcagaaccaa accaaacagt ctcacctgct
    25441 tgcatttggg caatcgccaa ttctttcaag cgctccattg gaacgttgat gtaacgaact
    25501 gcacggctac caaccacatt ccccaacatc tcaactgtgt aagattttcc gtaaggttta
    25561 tcagcagttg gagcattgat aacagaaacg tagtcttcta aaggaagatt gacatatttc
    25621 ttgtagaact cttgtggtgt gattcctttt tcacttttgt agttgttatc tttatcgcga
    25681 taagcaaagt caaacttgcg tggtggaagt cctaatgaca tagcaagaaa gttaaagatt
    25741 tcttgcaaga ggtcttcttt cttagcttga acagtcactt gatctgcacc agaaacaagc
    25801 aaatcacgca agatttgagc atcttgacga agcaatttat taaggatcgc atttagctca
    25861 cgactgctgc tagatgaaac agactcagga taaactgact taggcacgac accgtatttt
    25921 tcaaagaggg aaacgaccat atcccattga ccaccatctt gttgaggtgt ttggagtaag
    25981 aagctaacct tgcggctagt caattcttgg tctgaagtcg caatgacttg ctccaagaac
    26041 cagtttgatt tctcatactt atcccagaag aaagtgtggg cttgtgacaa ctcaaagttc
    26101 tccaatttgt attgcgagat gagtttgtgg cggaaggtgt tgagagccgc aaacatccag
    26161 caacgaccag acgctttctg gttagtgacc ttgtccttgg ttaaatccaa tgagaaaaca
    26221 ggtgtgttgt ctacatggct ttggcgacgt tccagagctg caaaaattcc gttgtggctg
    26281 gcagcatttt caatcgcttg gtattttaca tttgcttcat agttggcaaa tagtttatca
    26341 gtaaatgatt cttgaatcgc gttcatagat tcctcctttt attctacagt ctattataac
    26401 tcttttcaca aaggaagcaa ctaaaaaaca taaaaggtaa ggacattctc cctcaccttt
    26461 ttcgaattta tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtactc
    26521 aagtacagcc tgcggctagt ttcctagttt gctctttgat tttcattgag tattaaacta
    26581 aattaaataa tattagcgcg gagaatttct aattcttcct tggtcaagcg acgccattcc
    26641 cctcgttcca ggttctcatc taatactaaa gttcccatag tcaatcgttg caagtccacc
    26701 acttccttgc cacagtagcc caccatacgc ttgatctgat gaaacttccc ttctgcaatg
    26761 gtcacacgga tttggctctg attcttttct gcatctgtgg aaagaatctc cagtctagcg
    26821 ggctgacagg taaagtcttt gagcggaatg cccttgataa atgtctccac atcttcttgg
    26881 gtcataattc ccttgacctg agcctgataa gtcttgtcca catgacgctt gggcgaaaga
    26941 agaacatgag ccagctgacc attattggtc aagagcaaaa gaccatgcgt gtcaatatcc
    27001 aagcgtccta ctggaaaaac ttccttactc cgcgccaagt catccaacaa gtccagaacg
    27061 gttctgtgct tgggatcctc agtcgctgag ataactcctt tgggcttgtt catcatgtag
    27121 tagacaaact cttcatactc caacacttgc ccatcaaagc gaatctcatc tattttttca
    27181 tcaatctgca atttagctga tttttctttt ttaccattta cagtcacgcg cccagccttg
    27241 agcaagtttt tgacctcagt ccgacttccc accgcacagg caactaaaaa tttatctaat
    27301 ctcatagaac tattatatca tatcaaaagg aggctagtac aatgaccaac ctccttttcg
    27361 tttcatactc ttcaaaaatc tcttcaaacc gcgtcaacgt cgccttgccg tatatatgtt
    27421 actgacttcg tcagttctat ctgcaacctc aaaacagtgt ttttagctga cttcgtcagt
    27481 tctatctgca acctcaaagc agtgctttga gcaacctgcg gctagtttcc tagtttgctc
    27541 tttgattttc attgagtatc agatttagga aattaacttc ctcgtctcca aaaaatagct
    27601 aaaacaatca tggctcctaa aatcgctggg ataatagctg ttcctgataa aactggcccc
    27661 caagttccaa agagcaagtg acctagaaag gctccgatcc aaccgagaaa catttttcca
    27721 aaacatccca ttcgctctcc acgattggtc atagcacctg ctaaaaatcc cactaggaga
    27781 ccaacgaaca tacttcctaa catattatct ccttaatttg cccaattccc attacggaaa
    27841 agaggtaccc gcgttccatc ctcacgaata ccatcgatat ccatttggtt agaaccaatc
    27901 ataaagtcta cgtgaacatc tgaacggtta agccctgcag cttcaagctc ctcttcgctc
    27961 atctccgctc catcaacaac gctagtcgca taggctgcac cgatagccaa gtggtttgac
    28021 gcattttcat cgaaaagggt gttaaaaaag gtaatgcctg actgagaaat tggacttgga
    28081 tctggtacca aggcacattc acccaaggca cgcgcacccg cattttcaaa gacaaggtct
    28141 ttcataacct gatcaccctt ctcagcagtg atatctacga tttgtccatc cttaaaggtc
    28201 accttaatgc cttcaatgat atttccgttg tagctaagcg gttttgtaga agtgacataa
    28261 ccatctgcac gacggaagtc aggcgctgtg aagacctctt ctgtcggcat atttggcaag
    28321 aattcttcgc cctgtgcatt gacagcacca gctgattccc aaacgtggtt ctttggcaaa
    28381 ccaagtgtta aatctgttcc tggcgctgtg tagtgaaggg ctgaaaattg ctccttatta
    28441 agcatatcgg ccttgctctt gagaatggct gcatgttcct cccaagcctt aacaggatct
    28501 gcttcgtaga cacgacaagt tttgaagatt tgatcccaaa ggaaatcaac tgcttcttcg
    28561 tcgctcgcag catttgggaa gactttctta gcccactcaa gtccagcagc ggctgctaca
    28621 gtccagctaa ccttgttaga ttgagttgcg atacgcatag gcttcatggc aagtcccata
    28681 gctttagcag aagctgaaag cttgtcagcg tccactccgt tcaaggcacc tggatcagat
    28741 gaacgaaccc caagacggct agccttgttc tccaaaagat agttcatctc agcaatcttg
    28801 tattctggca cattgtccaa acgctccatc ggggcatgga ggaatttctc acggttaatc
    28861 acatcatctg tccactgaac gatgacctca tgcgcaccca aggcataagc ttctttcacg
    28921 attagatgtg ccaattcacg ttgctccaca tcaatagaga gagccaaagt gtgaccaggt
    28981 tgcacgttaa ttccgttcgc aaccaacaat ttcgcatatt tttctagatt ttctttaaaa
    29041 tttggtaaaa ccattctgtt ttctcttttc tatttttatt tttctttcaa agcagaaaag
    29101 attcctgcta aagcaatagc acctgacaag agtgctgtcg atagaagaat tgtacgatca
    29161 gcaagttcta attgatattc aaataccttc ttagccggct tatcttccgc aaatattctt
    29221 agtttcatga gaaccccctt aaaatagatt aaaataaatc actgtgactg cctgtcctca
    29281 gcaaatttaa aatcaattcc gacttgtcta ctttataaac caaaagccaa tctggctggg
    29341 tatggcattc acgaactcct tgaaaatgct tggatgccgt caatgaatga tcacgatatc
    29401 tggcaggatg ttctttttct tgaaccagaa aattcaaaac ttcttctaat aattctgcct
    29461 tcaaaccacg cttcatagcc aacttaaaat cttttttaaa ctgtttatga taacgaatct
    29521 taagcacgta agtcctccat caaatctgag actgattcaa aagactggct catattacga
    29581 ttttggtcta aatccgttaa cgcttggagc aacttccgat tttcgtctag tctaacatca
    29641 aaaggtaatc cctgatattg aattgcctga cgaaggaaaa tattaatagc tgttgtcata
    29701 tccattccca gattactaaa cacctgttgg gcctgctcct taacctcact atccagacgg
    29761 atgcttatac tcatctttga catactctca ccctctttcc atagactatt ttaacaaaaa
    29821 agaaagctaa tgtaaatcta ttggatatac gttagcctct tttaatagat tattaagcaa
    29881 ttttttaaaa caactcatca aacaaactca actggttatc ctctggcata tttccaagaa
    29941 tacccatctc atccatcttt tcaaccaagg ttgatgagag tccaccacgc ttgcgtagtt
    30001 ctgttttaga gaggaattct ccctcttcac gcgcccgcac cagttgcttg gcaacgttct
    30061 ctcccagacc atccattgct acaaatggtg ggataagggt atccccgtcg atgaggaact
    30121 ctgtcgcctg actacggtag agatctaatt taccaaactt gaaacctcgt tcccacatct
    30181 cattgacaat ctcaagagtt gtatagagat cgatttccac attagaggct tcattgttct
    30241 tccgtttttc agagatttct tccattctgc gcttgatgac ctccaagccc gcacccatgg
    30301 tcttgatatc aaaagcctta gcacgaatgg agaagtaagc acagtagtaa taaataggat
    30361 ggtgaacctt gaagtaagct acacgcaagg ccatcataac gtaggctgcc gcatgggcct
    30421 tagggaacat gtacttaatt ttcccacagg attcgatata ccactctggc accttattag
    30481 ccttcattgc ttcgatatag ccatttctct cctcttctga aatctttagc cacaaaccct
    30541 tacgtacccg ttccataatg gtaaaggcca tcttaggttc cagacccgca tgcatgaggt
    30601 aaaccatgat gtcgtcccga caaccgataa cagtcgatag gtccgctatt ccttgcttaa
    30661 tcagatcctg agcattcccc aaccaaacat cagtaccgtg ggacagacca gacagctgaa
    30721 gcaattccgc aaaggttgtc ggatgggttt cgtctaccat tccacgtacg aaatttgttc
    30781 caaactctgg aatccccaac atacccgtag gcgttccaat ttgttcaggt gttaccccta
    30841 gcacatcagt cccagaaaag agtgccatca cgccttcgtc atccatagga attttattag
    30901 ggtcaatacc agacaaatcc tgaagttttc gaatcatggt cggatcatca tgtcccagta
    30961 catcgagttt gaggacgttc tcatcgatat cgtggaagtt aaagtgagtg gtctgccatt
    31021 cagccgtgac atcatctgct ggatactgga caggcgtaaa atcgtagaca tccatgtagt
    31081 tcggaataac aacgattccc cccgggtgtt ggcctgttgt ccgcttgaca cccgccgctc
    31141 cttgagcgag gcgttctact tctgcatcac gataaaactt gccataatct cgctcgtaac
    31201 ccttgacaaa tccataggca gtcttggcag ctaccgtacc aactgttccc gcacggaagg
    31261 catattcttc accaaagata tcacgcacat ccaagtgggc gctaggctga tcttctcccg
    31321 agaagttcaa gtcaatatca ggaaccttat ccccatcaaa accaaggaag gtctcaaacg
    31381 gaatatcctg tccgttttta ctgagtttgt gaccacagtt tggacagtcc ttatggggca
    31441 tatcaaatcc tgaaccgtac gaaccatctg tgataaactc actgtactga cactgaccac
    31501 agacatagtg aggagagaga ggattgacct ccgtaattcc aatcatggtc gcaacgaaac
    31561 tagatccgac agacccacga gaaccaacca aataaccccg ttcattagaa cgttgcacca
    31621 gcatctgcga tgccagataa atcacagcaa atccattccc cagtatggat gttaattctt
    31681 tttcaatccg caaatcaaca atatctggca gcggatttcc ataaatctca aaagctttct
    31741 tataggtcaa ctcagcaact gtttcttcag ccttgtcgat gaaaggcgta tacaagtcac
    31801 ccttaacgac ttcaacgggt tcaaatattt ctgccaaggc attggtgttt tcaataacca
    31861 gtttacgagc cagttcctct cccaaaaagg caaattcatc caacatctca ttagtcgttc
    31921 gaaaatgagc ctttggaagt ggtgctggtt gggcatgttc accatgaccg atagttcggt
    31981 taatcatcgc accctgtccc aaactacgga cgataatttc acgataaatc tcttcttccg
    32041 gttcgatata gtgaacattt cccgtagcca aaacaggctt gccaaggcgg tctccaacct
    32101 ctatcaaact cttgataatg gtctggagtt cctccatatc cttgacctgc tctttagcaa
    32161 tcaagggcgc atagatagcc ggtggcatga cctcgataaa gtcataatac ttggccacct
    32221 caaccgccgc atccacacct tgagaaacga ccacgtcaaa aacttcaccc tctgaacagg
    32281 ctgaacctaa aatcaagccc tctcgatggg catctagaac cgttctcgga atccgtggca
    32341 ctccttcaaa atacttggta ttagacaagg aaaccagctt aaagatattt tttagaccta
    32401 cctgattctt gacatagatg gtcgcatgct tgatccgagc ttttttgtaa gaatctggac
    32461 tgattagatc aatgttgagt ctagctaaat cggtcacacc atgtttttct gctacctctt
    32521 tgataaagat gaaaagcaga cgaccagtcg cttccgcatc gtagttggcc atgtgatgat
    32581 gttccaaggc cacaccaaaa cgcttggtca aaggccccaa accatggcgt ttatactcag
    32641 gatagaggtt tctagcaaac tccagcgtat caataactgg ctgactaatc tttggaagac
    32701 catgacgctc ataattagca ttcataaagc caacgtcaaa ggtagcattg tgggcaacta
    32761 ggaccgtatc cttgcaaaat tcttggaatt cttgcaaaac ttgttctagt ggtttggcat
    32821 ttttgacatg atcatctgta attccagtta actctgtagt aaaagctgac aagggatgcc
    32881 caggattgat aaattcatca aattcagcaa taacattccc cttgtacatc ttagaggccg
    32941 caacctgaat caagtcatta tagatagctg aaagtcccgt cgtttccacg tcaaagacca
    33001 cgtaggttgc ttctgataag tccatctcca cttcgttata gacgataggg acacggtcct
    33061 ccacgatatt ggcttccatc ccatagatca gctggattcc cgctttctta gccgccttat
    33121 agccatgtgg aaaggactgg acattcccat ggtctgtgat agcaaccgcc ttgtgtcccc
    33181 acttagcagc tgttgcaaca atctcttcga cctctggcaa agcatccata gtcgacatgt
    33241 tagtatgagc atgaaactca acccgacgct caccttctgg catcaaatcc ttccgctcat
    33301 agtgaacaac ttcctgcaga tcctgtacgt tcataatcaa atcgcgtgtg aagttattca
    33361 tctccacatt ccctcgaact cggagccaag aattcttctt gatgaggtca aacttctggg
    33421 cctcttcctc gtttttaacc cacttttgca tagaaaaact tgaagtatag tccgtcattt
    33481 taaagttgat taaaacacga cctgttctag tcactttttg ttccacatca aaaacaaccc
    33541 cttcaaatac cagacgattt tcctctgtcg tcacttcgat cataggagta atctccgcct
    33601 tatccagctt gggtttagct gcagcttttt tcgcttgaaa atcaaagact ggtttctctt
    33661 ccgctggagg aggtgccatc tgctccagtt gttccatagc acggagcgct tcctcattgg
    33721 cagcttgaac aatctgctca ttttcagcat gaaaggcctc ttcctgctct tgggtcagga
    33781 catcattctt ctcgacttga cagttaaaag ttggaaaacc aaacttttca agttgtttgg
    33841 ctaaattagg aagatgattc ttcttaaaat gttccttatc aatcgcttca gatccttcaa
    33901 taaatagctg attaccctca gcacgaactt gcaaattttg ataaagggac ttaaaacctt
    33961 gactagcaca tggaccttca gagaaagcct ccctatagta ggactgcaag agctgatttg
    34021 aaaattcttg agaccgagcc ttaatttcaa aaacagcttt attgcctgtc ttagaaaatt
    34081 cttcgctcaa acctttcttt aattctaaaa agatttcaat cggtaaaata ttagaaaata
    34141 cgaaatgaaa ctcccatacc ttactaattt tatgaaccac aactcgctca atattggcct
    34201 gtgctaaagc aggagcctgt ctcatttcag caggcatccc caattgattc atcaaaattt
    34261 caaaactatt tgacattcat tttcctcaca ttattcttct actattttac catatttaga
    34321 ggtattttct aaagacaaaa ggaagccact aagtgacttc cttctagagt gaggacggat
    34381 tagtcttcac ctttattttt cttaataatt tcttcttgta ctgacttagg tacatcttcg
    34441 tagtggtcaa ataccatcat gaatgtacca cgtccttgag atgcagaacg aagaactgtt
    34501 gcgtaaccga acatttcagc aagtggaacg taagcacgaa cgatttggct gttaccgtgt
    34561 gcttccatac catctacacg tccacgacga gcagttacgt gacccataac atcaccaagg
    34621 ttttcttctg gaacagtgat tgttacaagc atcattggtt caaggatagc tggttgtgct
    34681 gatttagcag cttctttaag ggaaagtgaa gccgcaatct tgaaggcagt ttcagatgag
    34741 tcgacatcgt gatatgaacc atcataaagc ttagctttaa cgtcaaccat tgggtaacct
    34801 gcaagaacac cgttagccat agattctacc aaaccttttt caaccgctgg gataaattca
    34861 cgaggaacca caccaccgac gattgcgttt tcgaattcga atcctttacc ttcttcgttt
    34921 ggagtaaatt caatccacac atcaccgaat tgacctttac caccagactg acgtttgaag
    34981 aatccacgtg cttgagtaga agcgcggaat gtttcacggt aagatacttg aggcgcacct
    35041 acgttcgctt caactttgaa ctcacgacgc atacgatcaa caaggacgtc aaggtgaagt
    35101 tcacccatac ctgagataac tgtttcacca gtttcaacgt ttgtttcaac gcggaatgtt
    35161 ggatcttctt cagccaattt ttgaagggcg atacccatct tgtcttggtc agctttagat
    35221 tttggctcaa ccatcaattg gataactggt tctggaacgt tgattgactc aaggatgatt
    35281 ttagattttt catctgtcaa tgagtcacca gttgtagtat ctttcaaacc aacggcagca
    35341 gcgatatcac ctgagtaaac agtgtcgatt tcttgacggc tgttagcgtg catttgaagg
    35401 atacgtccga tacgttcacg tttaccttta gaagtattca atacgtatga acctgattga
    35461 agaacacctg agtaaacacg gaagaatgtc aaacgaccta cgaatgggtc agtcatgatc
    35521 ttgaaggcaa gagctgcaaa tggctcttcg tcagatgctg gacgaatttc ttcagcgtct
    35581 gtatctgggt taataccttt gattgctggg atgtcaagtg ggcttggaag gtagtcgata
    35641 accgcatcaa gcatcaattg aacacctttg tttttgaagg ctgaaccaca caatactggg
    35701 aagaattcaa cgttgatagt cgctttacgg ataccagctt tcaattcttc gttagtgatt
    35761 tcttcacctt cgaggtattt catcatcaat tcttcgtcag tttcagcaac tgcttcaatc
    35821 aatttttcac ggtattcttg agcttggtca aggtattcag ctgggatgtc ttcttcaagg
    35881 atatccgtac caaggtcgtt agtatagatt tcagctttca tcttgatcaa gtcaatgata
    35941 ccacggaagt catcttcaga accgactggc aattggattg ggtgtgcatt tgcttgaaga
    36001 cgatcgtgaa gtgtgcttac agagtaaagg aagtcagcac cgattttgtc cattttgttg
    36061 gcaaatacga tacgtggaac tccgtactca gttgcttgac gccaaactgt ttcagtttga
    36121 ggctcaacac ctgattgtga gtcaagaacg gtaaccgcac catccaatac acgaagagaa
    36181 cgttgtactt cgattgtgaa gtccacgtgt cctggtgtgt cgatgatgtt tacgcggtgg
    36241 ttgttccatt gagctgttgt cgcagcagat gtgatcgtga taccacgttc ttgctcttgc
    36301 tccatccagt ccatttgtga cgcaccttcg tgagtttcac cgattttgtg gattttacca
    36361 gtgtagtaaa gaatacgctc agtagttgtt gttttaccgg catcgacgtg agccatgata
    36421 ccgatattac gagttttttc aagtgaaaat tcgcgtgcca tgaggtttgt ttctcctatt
    36481 tatttttgat ttctattcta ttataacacg attttaataa aaacggatag gcaggaccta
    36541 cccgttctca atgttttcat gctattgttg gtttcaactt acgagatggt aagctgagtt
    36601 atagctaata ctaatcgatt tagctaattt gaacccgggc taaagttagt tagccgatgt
    36661 gagctggaac gggatgctgc gtgaaaaaga taaaactcct tgtattcgtc gaatactgcg
    36721 tcagtttcct attttcacct tgcatcctta ccgttcctag catcatgatt tagttgagct
    36781 caagctaaaa gtctggagaa aaagatgaat ctcattggtt gcaaccgcaa cctgcttcga
    36841 ttccctattt tctctgggac tttcttaacg ctttcgcatc ctatcttacc aacggaagtg
    36901 tgcgaatgca cggttagctt cagccatacg gtgagtatct tcacgtttct taactgctgc
    36961 accagtgttg ttagcagcat ccaagatttc ttttgcaaga cggtcttgca ttgtgtgttc
    37021 accacgaaga cgagcgattg ttaccaacca acgaagtcca agtgttgtac gacgttctgg
    37081 acgaacttca actgggactt ggtagttaga accaccaaca cgacgtgcac gtacttcaag
    37141 tacaggcatg atgttttcca tagctgtttc aaatacttca agtgcatcgt tgccagtagc
    37201 ttctttgatt tgctcaaaag caccgtaaac gattgaagca gcagtaccac gtttaccatc
    37261 aagcataacg cggttgataa gacgagtaac tagttgtgaa ttgtatagcg gatctggcaa
    37321 tacgtcacgt tttggagctc tatttttacg actcatttct ctttatcccc tttccttatg
    37381 cttttggacg tttagtaccg tatttagaac ggccttgttt acgatcgtta acacctgcag
    37441 tatcaagtgc accacggacg atatggtaac gtacccctgg aaggtctttt acacgtccac
    37501 cgcgaagaag caccacgctg tgctcctgca agttgtgtcc gatacctggg atgtaggcag
    37561 taacttcgat aaggttgctc aaacgtacac gagcgaattt acgaagggct gagttaggtt
    37621 ttttaggtgt cattgttcca acacgagttg caacaccacg tttttgtggt gaagaaacgt
    37681 ttgtttgaac ttttttatga ctattgtaac caacgttcaa agctggtgat ttagattttt
    37741 ctacttttga tttacgcggt ttgcgaacca attggttaat tgtaggcatc tacattctcc
    37801 tgtgtttttt tatttttggt gatgatacac ttggtgacag atatcatctg tgtgtacttt
    37861 tgcaacattt gtcagcacgt ccctgtacac tcttgagaga ccaaaagtaa aaagtaccgt
    37921 ctattattgt aacaaaattt tcccttgctt gtcaagatat ttttcttttt tattggtaat
    37981 gttagagatc aatttctgaa taaaaatagc tgggaacaca tcatcttcct aataatacgg
    38041 cttggcttat gtgacatttt tatctccgtc atttatactg atatataaaa tagtacagaa
    38101 aacttctcaa aagtaagaat ttttttcaca aatcaaagaa aacgcttgca tattgtttta
    38161 ctacatgcta tactgatata gaaatttatt gattggagaa tcattatgag aaaaacacca
    38221 tctcacactg agaaaaaaat ggtctacagc atccgttccc tcaaaaatgg aactggttct
    38281 gtccttatcg gcgcaagcct tgtcttgctt gccatggcta caccaactat ctcatccgac
    38341 gaaagtacac caaccactaa cgaacccaac aacagaaata caaccaccct tgcccaacct
    38401 cttactgata cagcagctgg ctctggtaag aacgaaagtg atatttcttc acctagaaat
    38461 gcaaacgctt ccctagagaa aacagaagaa aaacctgcaa cagagccaac tacttctact
    38521 agtccagtaa caactgaaac taaggcagaa gagcccatcg aagataacta cttccgtatc
    38581 catgtcaaaa aacttcctga agaaaacaag gatgctcaag gactatggac ttgggacgat
    38641 gttgaaaaac catctgaaaa ctggccaaac ggagctttgt ccttcaagga tgccaagaaa
    38701 gatgactacg gctattacct agatgtcaaa ttaaagggag aacaagccaa gaaaattagc
    38761 ttcctcatca acaatacagc tggaaaaaat ctaaccggcg ataaatctgt agaaaaacta
    38821 gttccaaaaa tgaacgaagc ttggttagac caagattaca aggttttctc ttacgagcca
    38881 cagcctgcag gaactgttcg cgtcaactac taccgcacag atggcaacta tgacaagaaa
    38941 tctctctggt actggggaga tgtaaaaaat ccaagtagcg ctcaatggcc tgacggaaca
    39001 gactttacgg ctacaggcaa atatggccgc tatatcgaca ttcctcttaa tgaagccgca
    39061 agagaatttg gatttttatt actagatgag agcaaacaag gagacgacgt gaaaatccgt
    39121 aaagaaaatt ataagttcac agatttgaaa aatcatagcc aaattttcct aaaagacgat
    39181 gatgaatcga tttacacaaa tccatactat gtccatgata tccgtatgac aggagcccaa
    39241 cacgtaggca cttctagcat tgaaagtagc ttttcaacac ttgtcggtgc taaaaaagaa
    39301 gatatcctca aacactccaa catcactaat cacctaggaa acaaggtaac tattaccgat
    39361 gttgcaatcg atgaagctgg taagaaagtg acctacagcg gagatttctc tgacacaaaa
    39421 catccttata ctgttagcta caattccgac caattcacta ccaaaacaag ctggcgcctg
    39481 aaagatgaga catacagcta tgatggcaaa ctgggagctg acctaaaaga agaaggaaaa
    39541 caagttgatt tgaccctttg gtcaccaagt gctgataagg tttctgttgt tgtctacgac
    39601 aagaatgacc ctgacaaagt agttggaact gtcgctctcg aaaaagggga aagaggaact
    39661 tggaaacaaa ctctagacag cacaaacaaa ctcggaatca cagatttcac tggctactat
    39721 tatcaatacc aaatcgagcg tcaaggtaaa actgttcttg cactcgatcc ttacgctaaa
    39781 tctcttgctg cttggaatag cgacgatgcc aaaattgacg atgcccataa agtggctaaa
    39841 gccgcctttg tagatccagc taaactcgga cctcaagact tgacttatgg taagattcac
    39901 aatttcaaga ctcgtgaaga cgccgttatc tacgaagctc atgtgcgtga cttcacttca
    39961 gatcctgcca ttgcaaaaga cttgaccaaa ccatttggga cttttgaagc cttcattgaa
    40021 aaactagact atctcaaaga cttgggtgta acccatatcc agctccttcc agtcttgtct
    40081 tactactttg tcaatgaatt gaaaaaccat gaacgcttgt ctgactacac ttcaagcaac
    40141 agcaactaca actggggata tgaccctcaa aactacttct ccttgactgg tatgtactca
    40201 agcgatccta agaatccaga aaaacgaatc gcagaattta aaaacctcat caacgaaatc
    40261 cacaaacgtg gtatgggagc tatcctagat gtcgtttata accacacagc caaagtcgat
    40321 atctttgaag acctagagcc aaactactac cactttatgg atgccgatgg cacacctcga
    40381 actagctttg gtggtggacg cttgggaaca acccaccata tgaccaaacg gctcctagtt
    40441 gactctatca aatacctagt tgatacctac aaagtggatg gattccgttt cgatatgatg
    40501 ggagaccatg acgccgcttc tatcgaagaa gcttacaagg ctgcacgcgc cctcaatcca
    40561 aacctcatca tgcttggtga aggttggaga acctatgccg gtgatgaaaa catgcctact
    40621 aaagctgctg accaagattg gatgaaacat accgatactg tcgctgtctt ttcagatgac
    40681 atccgtaaca acctcaaatc tggttatcca aacgaaggtc aacctgcctt tatcacaggt
    40741 ggcaagcgtg atgtcaacac catctttaaa aatctcattg ctcaaccaac taactttgaa
    40801 gctgacagcc ctggagatgt catccaatac atagcagccc atgataactt gaccctcttt
    40861 gacatcattg cccagtctat caaaaaagac ccaagcaagg ctgagaacta tgctgaaatc
    40921 caccgtcgtt tacgacttgg aaatctcatg gtcttgacag ctcaaggaac tccatttatc
    40981 cactccggtc aggaatatgg acgtactaaa caattccgtg acccagccta caagactcca
    41041 gtagcagagg ataaggttcc aaacaaatct cacttgttac gtgataagga cggcaaccca
    41101 tttgactatc cttacttcat ccatgattct tacgattcta gcgacgcagt caacaagttt
    41161 gactggacta aggctacaga tggtaaagct tatcctgaaa atgtcaagag ccgtgactat
    41221 atgaaaggtt tgattgccct tcgtcaatct acagatgcct tccgacttaa gagtctccaa
    41281 gatatcaaag accgtgtcca cctcatcact gtcccaggcc aaaatggtgt ggaaaaagag
    41341 gatgtagtga ttggctacca aatcactgct ccaaacggcg atatctacgc agtctttgtc
    41401 aatgcggatg aaaaagctcg cgaatttaat ttgggaactg cctttgcaca tctaagaaat
    41461 gcggaagttt tggcagatga aaaccaagca ggaccagtcg gaattgccaa cccgaaagga
    41521 cttgaatgga ctgaaaaagg cttgaaattg aatgccctta cagctactgt tcttcgagtc
    41581 tctcaaaatg gaactagcca tgagtcaact gcagaagaga aaccagactc aaccccttcc
    41641 aagcctgaac atcaaaatga agcttctcac cctgcacatc aagacccagc tccagaagct
    41701 agacctgatt ctactaaacc agatgccaaa gtagctgatg cggaaaataa acctagccaa
    41761 gctacagctg attcacaagc tgaacaacca gcacaagaag cacaagcatc atctgtaaaa
    41821 gaagcggttc gaaacgaatc ggtagaaaac tctagcaagg aaaatatacc tgcaacccca
    41881 gataaacaag ctgaacttcc aaatacagga atcaaaaacg aaaacaaact cctatttgca
    41941 ggaatcagcc tccttgcgct ccttggtctc ggtttcttac taaaaaataa aaaagagaac
    42001 taaactagcc ctcctataga aaaatccccc aagcattata gctcggggga ttaattttcg
    42061 tacaatattt gttgtcctaa taaacttgat taggattttt tattaagcct ctttcatagc
    42121 aaaataagct cgtactttgg gtgcaacttg tgttccgaag agttcaatag ctctcagaac
    42181 ctggtcatga ggcatagaac caagcggtag atgaagcatg aagcggtcca atcctaaatc
    42241 ctctatcatg cgaatcaatt tttcggccac ctgatctgga ttgccaacaa acatggcgcc
    42301 atttggccct acctgctcca aatattgctc ataacgcaat tcctgccagt gcggacggtc
    42361 tttggaaata gcatccacca cttgcttagt cggatggaaa taatctttca ccgcctgctc
    42421 accatcttcc gcaatccacc cccaagaatg ggctcccact ttcaagtctt tgtcagcatg
    42481 acccgcttcg cttccaatct cacgataagc ctgaatcaac tttttaaaat aacgtggatt
    42541 accaccaata atagcatata caatcggtag accagcctga gcaatcttca ctgttgattc
    42601 gacatgacca cctgtagcta tccacaaggg caatttgtcc tgaactggac gaggataaac
    42661 ttctttacca gcaatcgttt gagtcaatcg accttgccag tctaacttgg tcttttcatt
    42721 gactaactga agcaagtcta atttctcatc aaaaagagag tcgtagtctt tcaagtcata
    42781 accaaacaga gggaaagatt ccgtgaaaga gccccttcca gccataatct ccgctcgtcc
    42841 atttgacaaa gcatcgatag tggcatactg ttggaacaaa cgaatcgggt ccatgcttga
    42901 cagaatgctg actgcactgg tcaaacggat tttcttggta ttgactgccc cagcggccag
    42961 aacaatctct ggggctgata ctgcaaaatc cgcccgatgg tgctcaccaa tcccatatac
    43021 atccaaacca accttgtcag ccagctcaat ctctgccacc aactggcgaa tgcgttcagc
    43081 atgactgtaa gtttgtccag tcccttcaag ctccgttatt tccccaaatg ttgaaattcc
    43141 caattctacc attgtgattc tccttgtcta tctctgtcct tcaatttgaa aaattattct
    43201 aacacgaatc ttaagtacaa gcaaccaatt tgctcattag aaaaagccta gataactaga
    43261 cttttttagc ttattctacc gttactgatt tagcaaggtt acgtggttta tccacatcga
    43321 ggccacggtg gagggttgca aagtaagcga ctaattgcgt tggtacgacc attgaaattg
    43381 gtgagaggta tggatgtacg gtcgtaagga cgatatcgtc ggtatctttg gctacattct
    43441 cttctgcgat agtgaggact ttggcaccac gggctgcgac ctcttggata tttccacgag
    43501 tatggttggc aagaactgga tctgacaaga gagccaaaac aggcgttcct tcttcaatca
    43561 aggcaatggt tccgtgcttg agttctcctg ccgcaaaacc ttcacactgg atataagaaa
    43621 tctctttgag tttgagactt gcttccatgg ctacgtagta atcttgacca cgtccgatgt
    43681 aaaaggcgtt acgagttgtt tcaagaagtt cacgaacctt ggcttcaatg gtttctttct
    43741 ctgaaagagt tgattcgata gactgagcta cgattgacaa ttcatgaacc aggtcaaagg
    43801 cttgcgcttt agcattacca tttgcttctc cgactgcttt tgcaaggaag gcaagggctg
    43861 cgatttgcgc tgtataggct ttagttgatg ccacggcaat ttcaggacct gcgtgaagga
    43921 gcatggtata gttggcttca cgtgagaggg ttgaacctgg aacgtttgtc actgttaagc
    43981 ttggaattcc catttcatta gccttgacca aaacttgacg actatccgct gtttcaccag
    44041 attggctgat aaagatgaag agtggtttct tgctgagaag tggcatactg tagccccact
    44101 cagatgagat tccaagttca actggtgtat ctgtcaattc ttccaacatt ttcttagaag
    44161 caaatcctgc atggtaagat gttccagctg caaggatgta gatgcggtct gcgtcttgaa
    44221 cagccttaat gatatctggg tccacgacaa cttgaccagc ctcatctgtg taggcttgga
    44281 tgagtttccg cataacagtt ggttgctcgt caatttcctt gagcatgtag taagggtaag
    44341 ttcccttacc gatatctgac aagtcaagct cagcagtgta gctagcacgc tcacgacgat
    44401 ttccatcata gtcttgaact tccacactat cagccttgac gattaccaac tcttggtcat
    44461 ggatttccat gtattggtta gtttcacgaa tcatagccat ggcgtctgag cagaccatgt
    44521 tatagccttc tccaagacca atcaaaagtg gtgatttatt tttagctacg tagatgactt
    44581 caggatcttg tgagtcaacc aaggcaaagg cataagaacc acggatgatg tgaagggctt
    44641 ttttgaaggc ttcaagaact gagagccctt cttcttccgc aaattttcca atcaaatgaa
    44701 cggctatttc agtatctgtc tgccccttga agtggtgacc tgcaaggtat tcttccttga
    44761 tttcaagata gttctcaatc accccattat gcaccaagac aaaacgttct gtctcagagc
    44821 ggtgtgggtg agcattgtcc tcagttggtt ttccgtgagt agcccaacga gtatgtccga
    44881 taccagttgt tccctcaaca ccagctgtct tggcagacaa ttctgcaata cgaccaaccg
    44941 ccttcaccaa atggttatca gcaccatcta ggacaaaaat tcccgcagaa tcatagccac
    45001 ggtattcaag cttttcaagc ccttgaatca aaatatcagt tgcatttgtg tttccaacaa
    45061 caccaacaat tccacacata gtatatacga cacaggcaag ctgtgctttc tccttaaaat
    45121 tggtatagtc taattcatct tttatagaat cagcaaaaac agtatatact tgtttctttc
    45181 acttgtcaag agtaaaaatt ggtatagttc aaattaagct cctgtaagca aaaaactctg
    45241 accgattggg ataatcagtc agagtccttt ttaaaatcca ttattatcgc ttaattcttt
    45301 gaaccagtgg cctgatttct tcagacgacg ttcttgcgtt tccaagtcta attcgaccaa
    45361 accatagcga tttttatagc tgttgagcca tgaccagcag tcaataaagg tccaaatcaa
    45421 gtagccctta cagttggcac catcttcaat ggcacggtga agttcacgaa gatgaccttt
    45481 tacaaagtca atacggtaat catcttgaat cattccatct tgacggaatt tttcttcccc
    45541 ttcaacaccc ataccattct cagtcaacat ccactcaata ttgccataat tttccttgat
    45601 attttgggcg atgtcataaa tcccttgctc ataaatctcc caaccacggt gagaattgat
    45661 tttacgtcca ggcatcacat aaggctcgta aaaatgttct ggtaagagtg gactatctgg
    45721 atgcttagca aatcgaggag ccataacacg caaaggttga tagtagttca caccaaggaa
    45781 gtccaccgta ttatcacgaa tgagttccaa ctcttcctct gtagcatcag gtaaaagacc
    45841 gtgttcatgc aagatttcta ccaactcctg tggataagtc cccaagacag atggatctaa
    45901 gaaagattgg gcctgaaaaa gggccgcaat acgagctgcc ttgacatcag caggatgctg
    45961 gctacgtgga taagccggtg tcaagttgag gacaatccca atcttggaat caggcaaaag
    46021 ttcatggcaa gccttaacag cccggctgct ggccaattgt gtatgatagg ctaccttaac
    46081 agctgcctct gcatccacct tatgtggata atgggcatca taaaaataac caaattctac
    46141 aggaacgatg ggctcgttaa aggtaatcca ttgatccact aaatctccat aagtctcaaa
    46201 acaaaaacga gcatagtctt cataggctga gactgtcgcc ttattttccc aaccatcacc
    46261 atcctcttga agggcaaaag gtaaatcaaa atgatagaga ttgactaaca gacgaattcc
    46321 tttagcctta atagcctcaa agaccttacg ataaaaatcc acaccttgag ggttgacttt
    46381 tccacagcct tgtggaaaaa tccgtgacca ctgaatagaa gtccgaaaga ctgtgtgacc
    46441 agtctctaac aaaagctcaa tatcccgctc ccaattttca taaaaagtcg atgtcttatc
    46501 tgaaccaatc ccattatagt aacgatttgg ctccacttgg aaccagtaat cccagagatt
    46561 gtctccctta ccgtcaccag ctacacgtcc ttctgtctgc ggtccagaag tagaggatcc
    46621 ccagacaaaa tcctttggaa atcttagcat aacatttacc tctttatcta ctcatttctc
    46681 ccattataca gaaaaaacaa ggtaaaaact agttacattt tttccttgtt tttcttctga
    46741 ttatagtttt tatttcttgc ttaggatttc aagcgtttca agcacgttat ctgcatgaac
    46801 ctcaatggtg tcaccagttg ccttgatctt aacttctaca atgccatcgg ccgctttttt
    46861 accaacagtg atacggattg gaagaccaat caagtcacta tcgctaaatt taacaccgac
    46921 acgttcgtta cggtcatctg tcaagacttc ataaccagct cccatcaagc ttgcttcaag
    46981 tttttctgtc aaggcttgcg cttcttcatc cttgacattg acagtaatca aatgcacatc
    47041 aaatggtgcc aattctttag ggaaattgat tccccaagcg taacggtatt caccttttgg
    47101 cgttttgtta acaaagaggc gagcgtgttg ctccatcact gctgaaagaa gacggctgac
    47161 accgataccg taacatccca tgatgattgg cacagcacga ccattttcat ccaagacatc
    47221 tgctcccatg cttgctgagt agcgagttcc gagtttgaaa atatgaccga tctcaatacc
    47281 acgcgcaaag ttaaggacac cttgtccatc tggggaaatt tcaccctcac gaacttcacg
    47341 gatatccaca tattctgcag taaaatcacg gcctgggttc acaccagtca agtggtagcc
    47401 atcttcgtta gcaccgacaa ctgcattgcg aacatcttgc accttacgat ctgcaataat
    47461 tttaatattc tctggcaaac caactggtcc aagtgaacca aatcctgctt gaacaacatt
    47521 cgccacttct tcttcgctag caacgtcaaa gaaatctgct cccaagtgat ttttcaactt
    47581 gacttcgttg agttggtcat ttccaactag aagggctgca acaagctcac catctgcaat
    47641 gtagaagagg gttttaatcg tttgttcttc tggaacattg aggaaagctg caacttcatc
    47701 aattgattta acatctggcg ttgcaacacg agtaacttct tcttcagcga caacacggtt
    47761 gcttggtttg tactcgtttg ttgccatttc taagttagct gcatagctag actcacttga
    47821 gtaagcaatg gtatcttcac cagagactat ccatttgagc aattctgcct tgatttcttc
    47881 ttgcacttct gcaggaattt cgtcaaatga ggcaactgac ttgtccaaga caacccagcg
    47941 gtcaaggtct gtacgagcag atgtaatggc cataaattct tggctatcct taccacccat
    48001 ggctccaccg tcaccaataa tagccttgaa gtctaaacca ctacgagtga aaatacgctc
    48061 ataggctgct ttgtactcat cataaacact atccaaacta tcatagttag cgtggaaact
    48121 ataagcatcc ttcatgataa actcacgtgt acgaagaagt ccattacgtg ggcgtttttc
    48181 atcacgatac ttgggctgaa tttgataaag gttgagtggc aattgcttgt aagatttaac
    48241 agaatcacgg acaatagctg taaaggtttc ttcgtgagtt ggacctaaga taaagtctga
    48301 tttttcacgg tttttcagtt tgtaaaggtc ttcaccatag gtttcgtaac gacctgattc
    48361 acgccacaat tctgcactaa gaagggctgg agccaacatc tcaacagcac caatcttttc
    48421 gaattcttgg cgcatgatgt ttttagcttt ttcaatcaca cggttggcaa gtggtagata
    48481 agaataaaca cctgctgaaa cttggcgaac ataaccagca cgcaacataa gagcatggct
    48541 gataacttga gcatcgcttg gcatttcgcg aagcgttggg ataggcattt tactttgttt
    48601 cataatattc ctcgattatc taaaaaagag tcgcataatg tcattccaag tcacagcaat
    48661 catcaagaca accatgatga ccactccggc caaggtgaca taggtttcaa tttcttgttt
    48721 caatggtttg cggcggatgg cttctaggat attgagcaca atcttaccac catccaaggc
    48781 tggaatcgga ataagattaa aaatcccaat attgatggaa atcattgcca agaagtacaa
    48841 gatattttca attccatttt tagcagcatc actacttgcc ttaaagatag caacaggtcc
    48901 acccaacttg ttcaaatccg gttggaaaat cagatttttc agagctgaga gaattcggag
    48961 agctgagtca gcagcagttg taaaaccacc tacaaacatg gatagaaaat ctgacttaac
    49021 ccccggttga acacctagaa ggtaacgacc ttgactatct tcgggtgtaa cagtgacttg
    49081 tttgtcactc cccttttcag aaatagtcac atccaaagtc ggtgccgtct tatctttggt
    49141 ttctgtttcc acagcttgga tcaagctttc ccagttgcta acctcatgtg agccaatctt
    49201 ggtaatttgt gccgtttctg gtactcctac cttggccaag gcaccttggg gcatgatatg
    49261 gaactgattg gtatcaacat ctctgacacc accctgcata aagattaaaa cccaaaaaac
    49321 aacgacacct aagataaaat tgttcatagg acctgcaaaa ttggtaatca gtttgcccca
    49381 gatagtcgca ttttgatatt gaacatctaa aggtgcaatc cgaacctcag taccatctgc
    49441 ttccacaacc gttgcatcgt gatccactgc aaatgttttt tcttcttcca gaaccaatcc
    49501 tttgataaag agcttgtctt caaaatcaaa ctgggtcacc tgcataggga gggctgtttg
    49561 atccaatttt ttacctgaga gattgatgcg tttaacctta ccatcatcag caagtgtcaa
    49621 actaacaggc gttcctgtct tgatttcagt tgtatcatca ccccaaccgg ccatgcggac
    49681 atagccaccc agaggcaaga ttcgaatggt ataggccgtt ccatccttgc caatgtgagc
    49741 aaagatttta ggtcccatac cgatggcaaa ttcacgtact aaaatccctg atttcttggc
    49801 aaagtagaag tgcccgaact cgtgcaccac tacaataatc ccaaaaacca gaataaaggt
    49861 taaaattccg agcatagcgt ttcctccgtc ttttgattaa aagagtccaa ataagtgcat
    49921 gattggaaat acaagcaaca tactatcgaa acgatccaaa acaccaccat gtccagggat
    49981 aaatttccca gaatccttaa caccaaaatg acgtttgatc gaactttcta gtaaatcacc
    50041 aaattgtcca gcaatgctaa agaaaatagc aaagactgac atcttgtaaa ttccatatgg
    50101 aagagcaact gtactgtcaa ctatcataaa gataatggtt actaaaattg ctcctaaaat
    50161 accacccaag gcaccctcaa gggttttatt aggcgatacc cttggtgcta actttcgttt
    50221 cccatagttc atcccaacaa gataggcacc actgtctgtc gcccagacga tacacaaggc
    50281 taagagagcc ttgtccaaac ctgcaacacg agcatctagt aaagcattaa atccaaagcc
    50341 cacgtagaag ctcatagcaa gagggaaaac cgcatcctca atcgtataag acttgctaaa
    50401 aacggtcgtt cctaacatga ttgaaatcaa aacactatag gcaaccacat tcccatcaac
    50461 tggcaaaaaa gtcaggtaat tctccaaggg aatggtcaat gcaaaggttg caaagagggt
    50521 caagaggccc tccatcgtca tggtctctag acctctcatc ttcaaaagtt catgcatggc
    50581 tagcatggct atgattccga ttgctatctg aagcaagagg cccccaatca ttaaaattgg
    50641 taggaaaata gccagggcaa tccctgcaaa caaggttctt ttctgtaaat cctgggtcat
    50701 atttcctcct aaactcctcc aaatcggcga tgacgacgat tataggcaag aatagcttcc
    50761 tgcaaggccg cttcgtcaaa atcaggccat aaggtgtccg taaaataaag ctcactatag
    50821 gctccctgcc atggaaggaa attgctcaaa cgtaattctc cactagtacg gataatcaag
    50881 tctgggtctc gtaagtcctt aggcaaatgc tgagtaaaga gatagttacc aatcaattcc
    50941 tctgtgatgt cacctgggtt gattttggca tctaaaacat cctgggaaat caacttaagc
    51001 gcctgtgtaa tctcagcacg tccaccatag ttaagagcaa aattaagaat caatcctgtg
    51061 ttgttcttag tcaattcctc agccttggtt aaagcttcaa aggtttgctt aggcaggcgg
    51121 tctgtctccc caatcatttg aatcttaaca ttattcgcat gtagttccgg gacataatta
    51181 tcataaaact ctactggcaa gttcatgata aacttgactt cctgatctgg acgggtccag
    51241 ttttccgtag aaaaagcata gaccgtaata accttgacgc ccagtttgtt ggctgccttg
    51301 gtcacggttt gcaatgcttc catgcccgcc ttatgtccaa aaactcgcgg ttgcatacgt
    51361 tttttagccc aacggccatt gccatccatg atgatgccga tatgagcagg aacctgtgtc
    51421 ggaacctcta cttccacagc cttatctttc ttaaaaaatc caaacatgat cttattccta
    51481 ttcaaaaatc tatcgtttca ttataccata tttccccatt ttcttctatc actaagctat
    51541 ttattctcag gcaccatgcc catttttcaa aaaaataagc cgcctgattg ggcgacttta
    51601 tttttatagg gagattatta tgaaaaagtt ttaggagttt aagttaaggt cttcttaact
    51661 tatgaactta gtgtacactc cctagcttaa agtttcctta agtattttta aaaatcaaat
    51721 ttttccattt ctcctgccaa tttttcttgg ataaacgtgt ttgatagagt tccattcggt
    51781 cttcattttc taagaaatga ggagttggac gaacttgaaa attcaaaata tcctccaaac
    51841 cataaggtgc atagagttca aaatctgatt cttcattcaa gcgcagtcca actgccgtac
    51901 accgttctgg atacttactc atagcatcac gagaactggt ataggaagca gtgtgaggac
    51961 tgtgctgatg catatagacc tgatttttca attcccactg gtactgagga aaatcctctc
    52021 tcagcttttt ctccagtaat aaggtttcct cataagaaaa atctggatca aagaaaatca
    52081 catctatatc tgtttcatga tcaaaagggg atttgtctga caaaagattc cagatgaaat
    52141 ttctgacaga acctgctgcc aactacgagt ctttcaaacc aaggtctcgg atgatcgtca
    52201 gaatggccat catatctgga ttttctctaa aagcctctaa gatttcttgc ttatttttca
    52261 ctgtattcat aacctaagtg ctcatatgcc ttagcagtcg ccacccgtcc agaccgtgtc
    52321 cgcatgataa aacctttttg aatcaagtaa ggctcataca tgtcttcaac tgtctcacgc
    52381 tcttcggcga tattgacaga aagagttcct agaccaacag gtcctccact gtacatctca
    52441 atcatggtac gaaggatttt ttgatccaca tagtccaaac cttcatggtc aacatccagc
    52501 atagtcaaag ccttatcggt aataatatca tcgattaccc cattccccat tatctgggca
    52561 aaatcgcgca cgcgcttgag gagacgattg gcaatacgag gggttccacg actacgtagg
    52621 gccaactcag atgctgcctc atgagtgatt tccatctcaa aaatatctgc cgtccgctcg
    52681 acaatttctg tcaagtcagc atgggcataa tactccatat gacctgtaat cccaaaacgt
    52741 gcccgtagtg gatttgagag cataccagcc cgagtcgtcg caccaatcaa ggtaaaagga
    52801 ggcaactcca aatgaacact gcgactgcct tcaccagccc caatcataat atcgatgtag
    52861 aagtcctcca tggcactata aagcacttct tccactgaca tgggtaagcg atgaatctcg
    52921 tcaataaaga ggacatctcc aggctctaac tcattcaaaa tcgctaccaa atcacccgct
    52981 ttttcgataa caggaccaga cgtttgcttg agattgactc ccagttcatt ggcaatgaca
    53041 aaagccatgg ttgttttccc caaacctgga ggcccaaata agaggacatg atccagcgct
    53101 tcatctcgca ttttggcagc ttctataaag atttgaagct ggtccttaac cttatcctgc
    53161 ccaatatatt cacgtaaata ctgaggacgg agcgtgcgtt ctactaactc ctcatccccc
    53221 attatctcat tatctaaaat tctactcatg gctctattat atcaaaaaac caagccacaa
    53281 acaaaaaagc cacctgattg ggtgactcct aagtttagca cttatgtggt ataatattct
    53341 acgacactgc tacaccgcct acgaaaggag gtgagatagc ccatgatgga attagtactc
    53401 aaaactatca tcggaccaat tgtggtcggt gtcgttcttc gtatagtcga taaatggcta
    53461 aacaaggaca aatagtgtca aaaaagaccc caagcttatt tggtcgtgag cttggggtct
    53521 tttctagcct atgatataga actagtactc aattcctttt tattatccca tagttcacga
    53581 attttgtcaa aggtttacat tttcttcaac cactgtacga caagacggtt aagattaaga
    53641 gaacgttagg gattctatca atttcataga aattttgatt tcgtaaacga agagacaatc
    53701 ttacatgtca cttctcattt aatacgccac tactagacaa gcaaaatcat tattacagta
    53761 gttccagttc ttcaattaac agtcacttac aatcaaattg agtttgaact agctgaagcg
    53821 accacagacc tatttcttag ttatattcgc t
//
LOCUS       DAVLWA010000006        42243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLWA010000006 DAVLWA010000000
VERSION     DAVLWA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.006"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            185..2164
                     /locus_tag="VYN80_000323"
     CDS             185..2164
                     /locus_tag="VYN80_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540297.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2231..3727
                     /locus_tag="VYN80_000324"
     CDS             2231..3727
                     /locus_tag="VYN80_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540298.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3727..4653
                     /locus_tag="VYN80_000325"
     CDS             3727..4653
                     /locus_tag="VYN80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540299.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4662..5729
                     /locus_tag="VYN80_000326"
     CDS             4662..5729
                     /locus_tag="VYN80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540300.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5740..6666
                     /locus_tag="VYN80_000327"
     CDS             5740..6666
                     /locus_tag="VYN80_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540301.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7233..8093
                     /locus_tag="VYN80_000328"
     CDS             7233..8093
                     /locus_tag="VYN80_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540302.1"
                     /translation="MSQFITHQSLYATREGFVDELAKQGYVEGENIEIDLQNAQGEQR
                     NLKTISQQLAESSDVVLAIARPSAQSLANTTQTTPVIFSAVTDPVSAKLVESREHPGG
                     NVTGTSDQSSDAISTQINLIKKVLPKAKTIGILYTQSEPNSVVQKDEAKRLLKEKGFT
                     VVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQVSIKHKVPVFGGSTE
                     MIAVGGLYNYGTNYEELVRQTARMLIRVLKGEKPENIAVELPEKLELHTNQEMADALG
                     IDISKLEGKE"
     gene            8105..8998
                     /locus_tag="VYN80_000329"
     CDS             8105..8998
                     /locus_tag="VYN80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540303.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            9011..9814
                     /locus_tag="VYN80_000330"
     CDS             9011..9814
                     /locus_tag="VYN80_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1540304.1"
                     /translation="MTSLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(9847..10557)
                     /gene="treR"
                     /locus_tag="VYN80_000331"
     CDS             complement(9847..10557)
                     /gene="treR"
                     /locus_tag="VYN80_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1540305.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIREQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            10742..12709
                     /gene="treP"
                     /locus_tag="VYN80_000332"
     CDS             10742..12709
                     /gene="treP"
                     /locus_tag="VYN80_000332"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1540306.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            12887..14512
                     /gene="treC"
                     /locus_tag="VYN80_000333"
     CDS             12887..14512
                     /gene="treC"
                     /locus_tag="VYN80_000333"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1540307.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            14768..15016
                     /locus_tag="VYN80_000334"
     CDS             14768..15016
                     /locus_tag="VYN80_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1540308.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            15223..16017
                     /gene="racE"
                     /locus_tag="VYN80_000335"
     CDS             15223..16017
                     /gene="racE"
                     /locus_tag="VYN80_000335"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1540309.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            16014..16985
                     /locus_tag="VYN80_000336"
     CDS             16014..16985
                     /locus_tag="VYN80_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1540310.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            16961..17482
                     /locus_tag="VYN80_000337"
     CDS             16961..17482
                     /locus_tag="VYN80_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1540311.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17479..17940
                     /locus_tag="VYN80_000338"
     CDS             17479..17940
                     /locus_tag="VYN80_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1540312.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            17931..18671
                     /gene="xerD"
                     /locus_tag="VYN80_000339"
     CDS             17931..18671
                     /gene="xerD"
                     /locus_tag="VYN80_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1540313.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            18671..19399
                     /locus_tag="VYN80_000340"
     CDS             18671..19399
                     /locus_tag="VYN80_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1540314.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19418..19987
                     /locus_tag="VYN80_000341"
     CDS             19418..19987
                     /locus_tag="VYN80_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1540315.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            19974..20696
                     /locus_tag="VYN80_000342"
     CDS             19974..20696
                     /locus_tag="VYN80_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1540316.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            20696..20938
                     /gene="yidD"
                     /locus_tag="VYN80_000343"
     CDS             20696..20938
                     /gene="yidD"
                     /locus_tag="VYN80_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1540317.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(21163..22128)
                     /locus_tag="VYN80_000344"
     CDS             complement(21163..22128)
                     /locus_tag="VYN80_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540318.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLAEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(22224..22976)
                     /locus_tag="VYN80_000345"
     CDS             complement(22224..22976)
                     /locus_tag="VYN80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1540319.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINIASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(22973..23929)
                     /locus_tag="VYN80_000346"
     CDS             complement(22973..23929)
                     /locus_tag="VYN80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1540320.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATSLVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(23919..24878)
                     /locus_tag="VYN80_000347"
     CDS             complement(23919..24878)
                     /locus_tag="VYN80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540321.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(25175..25285)
                     /locus_tag="VYN80_000348"
     CDS             complement(25175..25285)
                     /locus_tag="VYN80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1540322.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            complement(25301..26002)
                     /gene="galE"
                     /locus_tag="VYN80_000349"
     CDS             complement(25301..26002)
                     /gene="galE"
                     /locus_tag="VYN80_000349"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1540323.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            26403..27467
                     /gene="pepA"
                     /locus_tag="VYN80_000350"
     CDS             26403..27467
                     /gene="pepA"
                     /locus_tag="VYN80_000350"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1540324.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27489..27785
                     /locus_tag="VYN80_000351"
     CDS             27489..27785
                     /locus_tag="VYN80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1540325.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            28467..28910
                     /locus_tag="VYN80_000352"
     CDS             28467..28910
                     /locus_tag="VYN80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1540326.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            28914..29678
                     /locus_tag="VYN80_000353"
     CDS             28914..29678
                     /locus_tag="VYN80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1540327.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            29693..30421
                     /locus_tag="VYN80_000354"
     CDS             29693..30421
                     /locus_tag="VYN80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540328.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            30414..31934
                     /locus_tag="VYN80_000355"
     CDS             30414..31934
                     /locus_tag="VYN80_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1540329.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITENQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            32279..33085
                     /locus_tag="VYN80_000356"
     CDS             32279..33085
                     /locus_tag="VYN80_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1540330.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(33251..33787)
                     /locus_tag="VYN80_000357"
     CDS             complement(33251..33787)
                     /locus_tag="VYN80_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1540331.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            33920..34810
                     /locus_tag="VYN80_000358"
     CDS             33920..34810
                     /locus_tag="VYN80_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1540332.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            35054..35407
                     /locus_tag="VYN80_000359"
     CDS             35054..35407
                     /locus_tag="VYN80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1540333.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            35408..36445
                     /locus_tag="VYN80_000360"
     CDS             35408..36445
                     /locus_tag="VYN80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1540334.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAAFECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(36492..37499)
                     /gene="galR"
                     /locus_tag="VYN80_000361"
     CDS             complement(36492..37499)
                     /gene="galR"
                     /locus_tag="VYN80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1540335.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            37663..38841
                     /locus_tag="VYN80_000362"
     CDS             37663..38841
                     /locus_tag="VYN80_000362"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1540336.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKETVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            38860..40341
                     /locus_tag="VYN80_000363"
     CDS             38860..40341
                     /locus_tag="VYN80_000363"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1540337.1"
                     /translation="MTLVDKFVTHVIFESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLEMIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            40411..40815
                     /locus_tag="VYN80_000364"
     CDS             40411..40815
                     /locus_tag="VYN80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1540338.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            40996..41760
                     /locus_tag="VYN80_000365"
     CDS             40996..41760
                     /locus_tag="VYN80_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV1540339.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            41757..42218
                     /locus_tag="VYN80_000366"
     CDS             41757..42218
                     /locus_tag="VYN80_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1540340.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
BASE COUNT        12263 a         7845 c         9732 g        12403 t
ORIGIN      
        1 tgtcaaattt cctattttca ctgtagtctt tgacacttcg aacgacaatt tgaatcatat
       61 attcaaaaaa aatgaaaaat ttgtgccaac aagcctaaat attctgaaaa tttgtttgat
      121 ttttaatgga taatgtgata taatggttgt caatctataa ttcacataaa cagaggagta
      181 gaaaatgaaa aaaaatcgtg tatttgctac agcaggtctt gttttattag cagcaggtgt
      241 acttgcagca tgcagttctt caaaatcatc tgattcatca gcccctaaag cttatggcta
      301 tgtttataca gcagacccag aaaccttgga ctacctgatt tcaagtaaaa atagtacaac
      361 agtagtgact tcaaatggga ttgatggttt attcactaac gataattacg gtaatcttgc
      421 tcctgcagtt gcagaggatt gggaagtctc taaggatggt ttgacctaca cttataagat
      481 tcgtaaaggg gttaaatggt ttacctctga tggagaagaa tatgcagagg tgacggctaa
      541 agatttcgtg aacggtttaa aacacgcagc agataaaaaa tcagaagcta tgtatttagc
      601 tgaaaattcg gttaaaggct tggcagatta tctatcagga acttcaacag atttttcaac
      661 agttggtgtc aaggcggttg atgattatac gttacaatac actttgaacc agcctgaacc
      721 gttctggaac tctaagttga cctattctat tttctggcct ctgaatgaag aattcgaaac
      781 atcaaaagga agcgattttg ctaaaccaac agatccgaca tccttgcttt ataatggtcc
      841 attcttgttg aaagggttga ctgcaaaatc ttctgtagag tttgtaaaaa atgagcaata
      901 ttgggataaa gaaaatgtcc acctagatac tatcaatcta gcttactatg atggatcaga
      961 tcaggagtcg ctagagcgta acttcactag tggagcttat agttatgccc gtctttaccc
     1021 taccagctcc aactattcta aggttgcaga agaatacaag gacaatatct attacacaca
     1081 atcaggctct gggattgctg gtctgggtgt gaatattgat cgccaaagtt acaactatac
     1141 ttctaaaact acagattcag agaaagtagc tactaagaag gcattgctta acaaagattt
     1201 ccgtcaagcc ttgaattttg ctcttgatcg ctcagcttac tcagctcaaa tcaatggtaa
     1261 agatggagca gctttagcag ttcgtaattt atttgtaaaa ccagactttg tttcagctgg
     1321 tgagaagacc tttggtgatt tagtcgctgc tcaacttcct gcttatggtg atgagtggaa
     1381 aggtgtgaat ttagctgatg ggcaggatgg tttattcaat gctgacaagg ccaaggcaga
     1441 gtttgcgaaa gctaagaaag ctttagaagc agacggcgtt cagtttccta ttcatctgga
     1501 cgttccagta gaccaagcat caaaaaacta tatatctcgt attcagtcct ttaaacaatc
     1561 tgtagaaaca gttcttggtg ttgaaaatgt cgttgttgat attcaacaaa tgacaagtga
     1621 tgaattcctt aatattactt actatgctgc caatgcttca tctgaggatt gggatgtatc
     1681 gggaggagtt tcatgggggc cagactatca agacccatct acttacctgg atattttaaa
     1741 aacaactagc agtgaaacta caaaaacata tttaggattt gataatccaa atagcccttc
     1801 agtagttcaa gttggtttga aagaatacga taaattagtt gatgaagctg ccaaagagac
     1861 aagcgacttg aatgtccgtt atgaaaaata tgcagcggct caagcatggt tgacagatag
     1921 ttcactcttt attcctgcta tggcttcttc tggtgcagca ccagtgcttt cacgaattgt
     1981 tccatttact ggagcttctg cgcaaacagg ctctaagggg tcagatgttt acttcaaata
     2041 tttgaaatta caagataaag cggtgactaa ggaagagtat gaaaaagctc gtgaaaaatg
     2101 gttgaaagaa aaagctgaat caaatgagaa agctcaaaaa gaattggcaa gtcatgtgaa
     2161 gtaaataatt tctaatagaa ctttctctcc atgaggagaa ggttcttttg ggatttttaa
     2221 aggaaataat atgaaaaaat atatttttat gcgtgttttg cggtcattgg tttcgatttt
     2281 cttagtaacg actttgacct acacgattat ctataccttg gttcctcgaa aattgatttt
     2341 caagcaggat cctaactata ataaaattgc gacaacggct gataaacgtg ataactatga
     2401 aaatactgtg tttgagcgta tgggctacat tgagtattac gatactaaag agttgcaaga
     2461 aaaggcaagt agcatggatt cttctgtaac agtagaagca aatgcgacca ataaagctat
     2521 ttatgaaaag tacatcaatc aattaggtca tggttggact ttgggagaat ttactgaaag
     2581 tggtcaattc tatgctactc gtgaaattcc aatttttgaa cgtgtttttc acttctatgc
     2641 taacttgatt gacattgacc atacaaataa aatccaagac cctgaaaatc cagacttgaa
     2701 acgctacctt cgttttgaaa acgatccagc tatcggatgg tcattggtcg gttcaggaac
     2761 taaacataaa tatctcttgt actttaacag tcagttccca tttgttcatc aaaactttgt
     2821 gaacttgaat ttaggtgact cttacccaac ctatgctaat acaccagttc ttcaggttat
     2881 tactcaaggt caaggacaaa ccaaaactgc ccaagttcag ttcccaacag gtaagaaaac
     2941 gtcttctgta aatatttact caagaaccta caagtcacct agtcaggctg actctcgtga
     3001 agtagctagc tatgggaaag atgatcctta tacagcgact gaaagtaatt accaatatcc
     3061 atctatgatt gtcagctctg ctattactgg tttgattggt ttggttcttg cctatgctct
     3121 tgccgtgcca cttggttcag ccatggctcg tttcaagaac acttggattg atagcctctc
     3181 aacaggggct ttgaccttct tgcttgctct tccaacgatt gccttggttt acatcgttcg
     3241 attgattgga tcatctattt cccttccaga ttcattccct atcttgggag ctggagattg
     3301 gcgttcttac gttttaccag cagtcatcct tggtttgttg ggtgctcctg gtacagccat
     3361 ttggattcgt cgttacatga ttgacttgca atctcaagac tttgttcgtt tcgctcgtgc
     3421 aaaaggtttg tctgaaaaag aaatttcaaa caaacacatc tttaaaaatg ccatggttcc
     3481 gctggtttca ggaattcctg gtgccattat tggggttatc ggtggtgcaa cccttactga
     3541 aacagtcttc gccttcccag gtatgggtaa aatgttgatt gactctgtaa aagcatctaa
     3601 taactctatg gtcgttggtc ttgtcttcat ctttacatgt atttctatct tctcacgtct
     3661 tttgggagat atttggatga ctattattga cccacgtatt aaattgactg agaaaggagg
     3721 caaataatgt ctacaatcga taaagaaaaa tttcagtttg taaaacgtga cgattttgcc
     3781 tctgaaacta ttgatgcgcc agcatattct tactggaaat cagtgtttaa acaatttatg
     3841 aagaaaaaat caactgtagt catgttggga atcttggtag ccatcatttt gataagtttc
     3901 atctacccaa tgttttctaa gtttgatttc aatgatgtca gcaaggtaaa cgactttagt
     3961 gttcgttata tcaagccaaa tgcggagcat tggttcggta ctgacagtaa cggtaaatcg
     4021 ctctttgacg gtgtctggtt cggagctcgt aactccatcc tcatttctgt gattgcgaca
     4081 gtgattaact tggttatcgg tgtttttgtc ggtggtattt ggggtatttc aaaatcagtt
     4141 gaccgtgtca tgatggaagt ttacaacgtc atctcaaaca tcccacctct tttgattgtt
     4201 attgtcttga cttactcaat cggagctgga ttctggaatc tgatttttgc catgagcgta
     4261 acaacatgga ttggtattgc cttcatgatc cgtgtgcaaa tcttgcgcta tcgtgacttg
     4321 gaatacaact tggcgtcacg tactttggga acaccaacct tgaagattgt tgccaaaaat
     4381 atcatgcctc aattggtatc tgttattgtg acaaccatga ctcaaatgct tccaagcttt
     4441 atctcatacg aagccttctt gtctttcttc ggtcttggat taccgattac agtgccaagt
     4501 ttgggtcgtt tgatttcgga ttattcacaa aacgtaacaa ccaatgctta cttgttctgg
     4561 attccattga caacccttgt cttggtatcc ttgtcccttt tcgtagttgg tcaaaactta
     4621 gcggatgcta gtgatccacg tacacataga taggagtaga aatgacaaaa gaaaaaaatg
     4681 taattttgac tgctcgcgat attgtcgtgg aatttgacgt tcgtgacaaa gtattgacag
     4741 ccattcgcgg cgtttccctt gaactagtcg aaggagaagt attagccttg gtaggtgagt
     4801 caggatcagg taaatctgtt ttgacaaaga ccttcacagg tatgctcgaa gaaaatggtc
     4861 gcattgccca aggtagtatt gactaccgtg gtcaggactt gacagcttta tcttctcaca
     4921 aggattggga acaaattcgt ggtgctaaga ttgcgactat cttccaggac ccaatgacta
     4981 gtttggaccc cattaaaaca attggtagtc agattacaga agttattgta aaacaccaag
     5041 gaaaaacagc taaagaagcg aaagaattgg ccattgacta catgaataag gttggcattc
     5101 cagacgcaga tagacgtttt aatgaatacc cattccaata ttctggagga atgcgtcaac
     5161 gtatcgttat tgcgattgcc cttgcctgcc gacctgatgt cttgatctgt gatgagccaa
     5221 caactgcctt ggatgtaact attcaagctc agattattga tttgctaaaa tctttacaaa
     5281 acgagtatca tttcacaaca atctttatta cccacgacct tggtgtggtg gcaagtattg
     5341 cggataaggt agcggttatg tatgcaggag aaatcgttga gtatggaacg gttgaggaag
     5401 tcttctatga ccctcgccat ccatatacat ggagtctctt gtctagcttg cctcagcttg
     5461 ctgatgataa aggggaactt tactcaatcc caggaacacc tccgtcactt tatactgacc
     5521 tgaaagggga tgcttttgct ttgcgttctg actacgcaat gcagattgac ttcgaacaaa
     5581 aagctcctca attctcagta tcaaagacac attgggctaa aacttggctt cttcatgagg
     5641 atgctccaaa agtagaaaaa ccagctgtga ttgcaaatct ccatgataag atccgtgaaa
     5701 aaatgggatt tgcccatctg gctgactagg aggaaggaaa tgtctgaaaa attagtagaa
     5761 atcaaagatt tagaaatttc cttcggtgaa ggaagtaaga agtttgtcgc ggttaaaaat
     5821 gctaacttct ttatcaacaa gggagaaact ttctcgcttg taggtgagtc cggtagtggg
     5881 aaaacaacta ttggtcgtgc tatcatcggt ctaaatgata caagtaatgg agatatcatt
     5941 tttgatggtc aaaagattaa tggtaagaaa tcgcgtgaac aagctgcgga attgattcgt
     6001 cgaatccaga tgattttcca agaccctgcc gcaagtttga atgaacgtgc gactgttgat
     6061 tatattattt ctgaaggtct ttacaatcac cgtttattta aggatgaaga agaacgtaaa
     6121 gagaaagttc aaaatattat ccgtgaagta ggtcttcttg ctgagcactt gactcgttac
     6181 cctcatgaat tctcaggcgg tcaacgtcaa cgtatcggta ttgcccgtgc cttggtcatg
     6241 caaccagact ttgttattgc agatgagcca atttcagcct tggacgtttc tgtacgtgcc
     6301 caagtcttga acttgctcaa aaaattccaa aaagagctcg gcctgaccta tctcttcatc
     6361 gcccatgact tgtcggttgt tcgctttatt tcagatcgta tcgcagttat ttacaagggt
     6421 gttattgtag aggttgcaga aacagaagaa ttgtttaaca atccaattca cccatatact
     6481 caagccttgc tttcagcggt accaatccca gatccaatct tggaacgtaa gaaggtcttg
     6541 aaggtttacg acccaagtca acacgactat gagactgata agccatctat ggtagaaatc
     6601 cgtccaggtc actatgtttg ggcgaaccaa gccgaattgg cacgttatca aaaaggacta
     6661 aactagtaat ggttttataa tttccatgtc aatttttatg gaaattataa aacttttttt
     6721 gttggactga tttgaaaaac tttgaaagct tcctgaaaga atttttgaaa aattttaaaa
     6781 attatgaaat gttattgaca ggtgatgaaa aaggtggtag gacaggaagc atcaaaaaag
     6841 aaagcagaaa aagaaatgaa taagagacaa gccgtaacta tgaatcaaaa cttttacttt
     6901 agctacttta gatagtgttt gtagacatgt caccatggat gcaaacagtt caagcaattt
     6961 gtttgtatct atgtggctaa gatttttgat tgtggtccat atagatgaat atctaaatgg
     7021 actagctaag ttgtaacttg ttaggttatt tcaagtatcc gtttagatag tatctaggcg
     7081 gttttttgtt ttatcttttc tgagaaggag tttcattatg aaagttgttc gaaaatcact
     7141 agccccctct tggtagtggg gctcctcttg acttctctga tcagtttgca tcagttgaag
     7201 gcagataaga aaaagatgtc tttcgtatcg gtatttcgca atttattacc caccagtcct
     7261 tatacgctac tagagaagga tttgtggatg agctggccaa acaaggctat gttgaggggg
     7321 aaaatattga gattgatttg caaaatgcac agggagaaca aagaaatcta aaaacgattt
     7381 cccagcaact agcagaatcc agcgatgtcg ttctagccat cgcaaggcct tctgctcaga
     7441 gcttggccaa tacgacgcaa acgacaccag ttatcttttc agctgtaaca gacccagtca
     7501 gtgccaagtt ggttgagtca agagaacatc ctgggggcaa tgtaactggg acaagcgatc
     7561 agtcatcaga tgccatttca acccaaatca atttgataaa gaaagtgttg ccaaaggcta
     7621 aaacgattgg aatcctctat actcagagcg agcccaattc ggttgttcaa aaggatgaag
     7681 ctaagcgact tctgaaagaa aaaggcttta ccgttgttga aaaaacaatc ttggacagta
     7741 ataacgtcaa ggcggcagca gagagtttga tggcagaggt ggatatggtt tttgtcccaa
     7801 ctgataatat catttcgtta accatggaaa cagttaagca ggtttctatt aaacacaagg
     7861 ttccagtatt cggtggttca acagaaatga ttgctgttgg tggcttgtat aactacggaa
     7921 ccaattatga agaattagta cgtcagacag ctcgcatgct gattcgtgtt ttaaaaggtg
     7981 agaaaccaga aaatatagca gttgagttgc ctgaaaaact ggaattgcat accaatcaag
     8041 aaatggcaga tgcattggga attgatatta gtaagttaga aggcaaggaa taaggaggat
     8101 taagatgaat tttgtgttat ctagtttatc agaaggtttg ttgtggtcga ttgtggcgat
     8161 tggggtctac ttgactttcc gtattttgga tattgcggat atgactgctg agggagcctt
     8221 tcctctgggg gcagcagtcg ttgtatcaca gatacaggca ggggcaaatc cttggcttgc
     8281 gactttactt gctttgctgg caggtatggt agcaggtctt gtatcaggaa tgctccacac
     8341 caagatgaaa atcccagctc tcttgacagg gattgtgacc ttgacagggc tctattcaat
     8401 caatattaaa atcatgggca gtgtacccaa tctttccttg ggagattctg caaccgtctt
     8461 taaacagttg gcgagcttgg gtctgacaaa tgaaggaact gttttctcac ttagtttggc
     8521 ctgtttttta cttgtttgtt tggtcttgac ccttttgatg aaaacagaga ttggcttggt
     8581 cttgcgttcg actggggata atatcccgat gagtgaggcc aatggagtca atgtagacac
     8641 catgaagatt gttggttaca tgatttcaaa cggtttgatt gctctatgtg gttccttgtt
     8701 tgcccaaaat gatggatttt cagatgtgac ttctgggaca ggaaccattg ttgttggttt
     8761 gagctcggtg attattgcgg aagtcttgat acacgacttg accattggag gccgcttatt
     8821 atccatcgga atcggtgcta ttgtttaccg tttgattatt ttaaatatct atgaaattcc
     8881 aaatctagat caaaacttgg tacgtctctt taatgcaatc ttgctcgctt tggttttatt
     8941 tgcaccagaa ttgcaaaaga gattaaagat tcgtggtctg aaattgagaa atgaatagga
     9001 ggagaaagtt atgacaagtt tgttaacact tgaaaatatt cacaagacat ttgaagcagg
     9061 gacggtcaat gagaaccatg tcctcaaagg attagattta gaggttgaag agggagattt
     9121 tatctctgtg attggtggaa atggagcagg aaaatccact ttgatgaata tcttggctgg
     9181 caatctatcg gtggacgaag gggaccttct actggcaggg aaatccatta aaaatctgag
     9241 tgtcagaaag agagccaagg atattgcccg tgtttttcaa gatcctaaga tgggaacagc
     9301 ttctcgtttg acgattgagg agaatatggc tattgccttg agacgcgggc agaagagagg
     9361 gcttggttgg ggcgtgaagg agaaggatag aatccagttc caagaggcct tgaaggagtt
     9421 gaatattggt cttgaaaacc gcttaaaagt agatactcaa tatctctcag gaggacaaag
     9481 acaggctttg actctagtca tgacagatct gatgaaacca aaacttttac ttttggatga
     9541 acatactgcg gcacttgacc cgaaaaccag tcaaatggtg atggacttga cgcaaaagat
     9601 tgtggagcac catcagtgga cgactttgat gattacccat gatatgaatc atgcgattga
     9661 gtacggcaat cgtctcatta tgctctatca aggcaagata gtggtggatg tcaagggaga
     9721 agagaaaaag catctgacag ttgaagacct tatgcatctc ttccagaaaa atagtggcca
     9781 aagtctggtc agtgatgaat tagttttggg ataaagaaaa aggctgagat ctggtgtctc
     9841 agtcttttat ttcttgcgtt ttgcaaaatc tacaaatcta aatttctcta acttatggcg
     9901 actttcggta aactgaaatt ggcgtccgtc ttggagatag acttttgatt taatcgaaac
     9961 gacataaggg tctttgccaa tgtccatgag aatcttgtct cggtcgcttg agtggtcaat
    10021 ggtgatttcc ttctgagcat aatcaataag gagtttgagg ccattttcta tataagaata
    10081 gatagactgc tcagcaattt ggcgagtgag atttggaatt agttccatat ccagatagtc
    10141 cgtatccaga acggatacca gatcatccac cacacgctgg cggaccacct tccaaaccat
    10201 ccgaaactct gggaaaccgg ttatcagtga ggatttttta tcaataataa tcttgtccag
    10261 actgaccacg ttggttttag agcgcagtcc aagttcttta actagttctt ggtagctggt
    10321 taggttggat acagggaaat tgacggtttc ttctttgacg acttgagaac cttgctctct
    10381 tatctttttg atcaatcctt cctcttggag gagagacagg gcctttcgga cggtatcacg
    10441 actcacttga tattgctcca taaggtcgtg ctcgctagga aggaaatctc cgacagcgta
    10501 agtctcgttt tgaatggttt cttggatttg cttaaataat tgttggtatt tcttcatttt
    10561 gcctgcttta ataggttttt agtctagttt aatgaacacc tgtatctatt ttaccataca
    10621 agtgctagaa aatttacaaa aaaacaccct ttttatacca aacaagttgc agacaagttt
    10681 ggtatcgttt acaatatact tatcaaatca aacttgcttt gacaagtata aggagaatca
    10741 aatgggaaaa tttgaacaag aagccaaaga tctgcttcag gcaattggag gcaaagaaaa
    10801 tgtgactgcc gtaactcact gtgcgacacg gatgcgcttt gttttaggag atgataagaa
    10861 ggctaatgtt aaagctatcg agtcaattcc agctgttaaa ggaactttta caaatgcagg
    10921 tcaatttcag gtaatcattg gaaatgacgt gcccatcttt tataatgatt ttacagccgt
    10981 ttcaggtatt gagggtgttt ccaaagaagc agccaagtct gcagctaaga gtaatcaaaa
    11041 cgtggtccaa cgtgttatga ccactctggc ggagattttt actccgatta ttccagcctt
    11101 gatagtcgga ggattgatcc tcggtttccg taatgtcttg gaaggtgtcc attggtcgat
    11161 gttggatggc aagaccatca cagaatcctc tcagttttgg gcaggtgtca atcacttcct
    11221 ctggttgcct ggtgaagcta tcttccagtt cttaccagta gggattactt ggtctgtttc
    11281 tcgtaagatg ggaaccagcc aaattttggg aattgttctc ggaatctgtt tggtatcgcc
    11341 tcagttgctc aatgcctatg cggttgcttc aacgccagca gctgatatcg cggcaaactg
    11401 ggtttggaat tttggctatt ttactgttaa tcgtatcggt taccaagccc aagttatccc
    11461 agccttgctt gcaggtttga gtctgtctta tcttgaaatc ttctggcgca agcatatccc
    11521 agaagtcatt tctatgattt ttgtaccttt cttgtcattg attccagcct tgattttggc
    11581 tcatactgtt ttgggaccaa tcggttggac aattggacaa ggactttcat cagttgtctt
    11641 ggcaggttta actggtccag ttaaagggct cttcggtgca atttttggtg ccctctacgc
    11701 tccatttgtc atcacaggtc tgcaccatat gaccaatgcc attgatacac aattgattgc
    11761 ggatgctggt ggcactgccc tatggccaat gattgctctt tctaatattg ctcaaggctc
    11821 agccgtgttt gcctattatt tcatgcatcg ccatgatgag cgtgaggctc aggtttcact
    11881 tcctgcaacc atttcagcct atctcggtgt tacagaacca gctctttttg gggttaacgt
    11941 aaaatatatt tatccatttg ttgctgggat gactggttca gcccttgcag gcatgttatc
    12001 cgttactttt aatgtaactg cggcttctat tggtatcggt ggtttgccag gtattctctc
    12061 tattcaacct caatacatgc tgccatttgc aggaactatg ctagttgcga ttgttgttcc
    12121 aatgctcttg actttcttct tccgcaaggc tggtctcttt acaaaaacag agggcgatac
    12181 gaacttgcag gcagaattcg ttgctcaaga agaagcagaa tttgtgaacc atgaaccagt
    12241 agaacttact tcggtagaaa ttatcagccc actaactggc caagtgaaag aattgagtca
    12301 agcgacggat cctatttttg catcaggtgt catggggcaa ggtctggtca ttgaaccaag
    12361 ccaaggtgag ttgacctctc cagttaatgg gacagtgacg gttcttttcc ctaccaagca
    12421 tgccatcggc attgtctctg acgagggagt tgaattgctc atccacatcg gtatggatac
    12481 agtaggtctt gatggcaaag gttttgaaag tcttgtagtc caaggagatc acgttacagt
    12541 tggtcagcaa ctgattcgtt ttgatatgga tgtcattaag gctgcaggtc tggtgacaga
    12601 aactcctgtt atcatcacca accaagatgc ttatacagcg actattcccg gaacttatcc
    12661 gacaacgatt caagctggag catctctcat ggtcgctaca cgaatctaat actcttcgaa
    12721 aatctcttca aaccacgtca gctctatctg caacctcaaa acagtgtttt gagcaacctg
    12781 tggctagctt cctagtttgc tctttgattt tcattgagta taattgaaat agcacaaata
    12841 gggacggaag actgcttcct cccttttatc agaaaaggag aaacagatga cacttgataa
    12901 aggaaaagta gtctatcaaa tctatccaaa atcttacaaa gacaccactg aaaacggtat
    12961 tggggatttc cgtgggatta tcgaaaaaat tccctattta gccaaactag gcgtggatat
    13021 ggtctggctc aatccattct atccaagccc tcaacgggat aatggttacg atatttcaga
    13081 ttatatggca gtggatcctc tttttggtga tatggctgat tttgaggaaa tggtgtgtgt
    13141 cggtaaagag cacaagattg actttatgct ggacatggta cttaatcatt gttcgacaga
    13201 gcatgaatgg tttcagaaag ccctagctgg tgataagtat tatcaagact ttttcttcat
    13261 ccaagaccaa ccaacagatt ggcagtctaa gtttggtggt tctgcatggg cgcctttcgg
    13321 ggatactggg aaatactatc ttcacctatt tgatgtgacc caggctgacc ttaactggcg
    13381 caattccaat gtccgtaagg agcttttcaa ggttgttaat ttctggcgcg acaagggtgt
    13441 caaaggtttc cgatttgatg tgatcaattt gatcggtaag gacgaggttt cagtggactg
    13501 tcctgaaaat gaagggaagc cagcttatac agacaaaccc atcgttcata actatttgcg
    13561 tatgatgaac caagccactt ttggatctga cgatagcttt atgacagttg gggaaatgtc
    13621 ttctaccacc atggaaaact gtgtcctcta ttcgtcgcct gatcgtcagg aattatccat
    13681 gacctttaat tttcatcacc tcaaggtgga ctataaggat ggacaaaagt ggaccttggc
    13741 tccctttgat tttgaagagt tgaagagtct ttaccatagt tggggtaagg aaatgagtga
    13801 taaagacggt tggagtgccc tcttttggaa caatcacgat caaccacgtg ccttgaaccg
    13861 ttttgtcgat attcaaaact tccgcaagga aggagccacc atgctagcgg ccagcattca
    13921 cctgtcacgt gggacacctt atatctacat gggtgaggaa atcgggatga ttgacccaga
    13981 ctatgattct atggctgatt atgtggatgt cgaatcgctt aatgcctatc agatgctctt
    14041 agaagaagga aagagtcagc aagaagcctt ccagattatt caagctaagt cgcgtgataa
    14101 ttcacgcatt cccatgcagt gggatgcttc ggaaaatgca ggattttcaa caggcactcc
    14161 ttggttgaaa gctggtaaat cctataaaga catcaatgta gaaaatgaaa tccagggtcc
    14221 gatttttacc ttctatcaag acttgattcg actgcgtaag gaaatgccaa tcatttcaga
    14281 aggaagttac aaaccagcct ttgaagatag caaacaagtc tacgcttttg aacgccagtt
    14341 tgaggatcaa aagttactag tgctcaataa cttttatgct aaagaagtgg aaatcgactt
    14401 gccagcagtc taccaaaatg gacagatttt gatttcaaac tatgaagatg cagaagtgtc
    14461 tgagaaaatt ctactcaaac cctatcaaac actggctatt tatgtaaatt aatactcttc
    14521 gaaaatctct tcaaaccgtg tcagttttat ctgcaacctc aaagcggtgc tttgagcaac
    14581 ctgtggctag cttcctagtt tgctctttga ttttcattga gtataaactt agcaaggaga
    14641 cctctttttc agaaaagcag ggttcttttt gctgtctatt ccatccttga aagtgtagaa
    14701 caaactggtc agggtggcta gtttgtggta taatgaaagg gactcaaaaa gaaacaggag
    14761 aaaaatgatg gatttacttt tagcaattgt attgattgtg ctagcttttc taggaggagc
    14821 tcttggagga atgtacttgg ttcgtaagca aatcgaaaaa gaatttgctg acaacccacg
    14881 tttgaatgct gaagcagttc gtactctttt gagtgcaaat ggtcaaaaac caagcgaagc
    14941 taaggtacaa caagtttacc accaaatcat ccgccaacaa aaggcagccc ttgctaacaa
    15001 taaaaagaaa aaataaataa ggaaaagtct gggatgaaag ttccagactt ctcactatgt
    15061 tggttatggt ttagggatga gactttttcc ttgcattcta ttgatatttg attatgatta
    15121 agagagaggt agttgacatt ctatcaagct gtcggtcagt tcgttcagta caaggaatca
    15181 taatgaacga tcaatcagaa aaaagactag aaagaagact gtatggataa tcgaccaatt
    15241 ggttttttgg attcgggtgt cgggggcttg accgttgtgc gcgagctcat gcgccagctt
    15301 ccccatgaag aaatcgtcta tattggagat tcggcgcggg cgccctatgg cccccgtcct
    15361 gctgagcaaa ttcgtgaata tacttggcag ctggtcaact ttctcttgac caaggatgtc
    15421 aaaatgattg tcattgcttg taacactgcg actgcggtcg tctgggaaga aatcaaggct
    15481 caactagata ttcctgtctt gggtgtaatt ttgccaggag cttcggcagc catcaagtcc
    15541 agtcaaggtg ggaaaatcgg agtgattgga acgcccatga cggtacaatc agacatatac
    15601 cgtcagaaaa tccatgatct ggatcccgac ttacaggtgg agagcttggc ctgtcccaag
    15661 tttgctccct tggttgagtc aggtgccctg tcaaccagtg ttaccaagaa ggtggtctat
    15721 gaaaccctgc gtcccttggt tggaaaggtg gatagcctga ttttgggctg tactcattat
    15781 ccactccttc gccctattat ccaaaatgtg atggggccaa aggttcagct catcgatagt
    15841 ggggcagagt gcgtacggga tatctcagtc ttactcaatt attttgaaat caatcgtggt
    15901 cgcgatgctg gaccactcca tcaccgtttt tacacaacag ccagtagcca aagttttgca
    15961 caaattggtg aagaatggct ggaaaaagag attcatgtgg agcatgtaga attatgacaa
    16021 ataaaattta tgaatataag gatgaccagg actggtatgt tgggtcttat agtatttttg
    16081 gtggcgttaa cagtttgagc gactataaga cagattttcc tctgtttgaa ttctccaaaa
    16141 tatttggaga tgaagagtat ggtttcccgc tttcagttac tgttttacgc tatggttcta
    16201 cctaccgttt gttctccttt gtggtagata tgcttaatca agaaatggga cgaaacttgg
    16261 aagttattca acgtcatggg gccctgctct tggttgaaaa tgggcaactc ttgtatgtag
    16321 aattgcctaa agaaggggtc aatgttcatg atttctttga gacaagcaag gtcagagaaa
    16381 ccttgttgat tgcgactcgt aacgaaggca aaaccaagga attccgagct atctttgata
    16441 agttaggcta cgatgtggaa aatcttaatg actaccctga cctgcctgaa gtagcagaaa
    16501 caggtatgac ctttgaagaa aatgcccgcc ttaaggcaga aaccatttct caattaacgg
    16561 gtaagatggt tttggcagat gattctggtc tcaaagtcga tgtccttggt ggcttaccag
    16621 gcgtctggtc agctcgtttc gcaggtgtgg gagcaactga ccgtgaaaat aatgccaaac
    16681 tcttgcacga attggccatg gtctttgaac tcaaggaccg ctcggctcag ttccacacaa
    16741 ccctagtcgt agccagccca aataaggaaa gtttagttgt tgaagcagac tggtcaggtt
    16801 atattaactt tgaacctaag ggtgaaaatg gctttggcta tgatcccctc ttccttgtag
    16861 gagagacagg tgagtcatca gctgaattaa ccctagaaga aaaaaatagt caatctcacc
    16921 gtgccttagc cgttaagaaa cttttggagg tatttccatc atggcaaagc aaaccatcat
    16981 tgtaatgagc gattcccatg gcgatagctt gattgtggaa gaagtccgtg atcgctatgt
    17041 gggcaaagtc gatgccgttt ttcataacgg cgattctgaa ctacgtccgg attctccact
    17101 ttgggagggc atccgcgttg ttaaagggaa catggacttc tacgccggct acccagaacg
    17161 tctggtgact gagcttggtt cgaccaagat tatccaaact catggtcact tgtttgacat
    17221 caatttcaac tttcaaaagt tggactactg ggctcaggag gaagaggccg ctatctgcct
    17281 ctatggtcac ttgcatgtgc caagtgcttg gttggaaggc aagatcctct ttctaaatcc
    17341 aggttctatc agtcaaccac gaggtaccat cagagaatgt ctctatgctc gtgtggagat
    17401 tgatgatagt tacttcaaag tggacttttt gacacgagat cacgaggtgt atccaggttt
    17461 gtccaaggag tttagccgat gattgccaag gagtttgaga ctttcttgtt ggggcaggag
    17521 gaaacttttt tgacccctgc taaaaatcta gctgtgttga ttgataccca caatgcggat
    17581 catgcgaccc tcttgctcag tcagatgacc tatacccgtg ttcccgttgt gacagatgaa
    17641 aaacagtttg ttgggacgat tggactcaga gatattatgg cttatcagat ggagcatgac
    17701 ttgagccaag aaatcatggc ggatacggat atcgttcata tgacaaaaac ggacgtagcg
    17761 gttgtttcgc ctgatttcac cattacggag gtcttgcaca agctagtaga tgagtccttc
    17821 ttaccggtcg tggatgcaga gggtattttc caagggatta ttacgcgcaa gtccatcctc
    17881 aaggccgtta atgccctctt gcatgacttt agtaaggaat atgagattcg atgccaatga
    17941 gagacaggat ttcagccttt ttagaggaaa agcagggctt gtctgtcaat tccaagcagt
    18001 cctataagta tgatttggag caatttttag acatggtagg tgagcggatt tctgagacca
    18061 gtctcaagat ttaccaagcc cagctagcca atctaaaaat cagcgcccag aagcgaaaga
    18121 tttcggcctg taaccaattt ctatactttc tctatcaaaa aggagaggtg gacagctttt
    18181 accgcttgga attagccaaa caagctgaaa agaagacgga aaagccagag attctatacc
    18241 tagactcttt ttggcaggaa agcgaccatc cagagggccg cttgctagcg ctcttaatcc
    18301 tagaaatggg gctcttgccc agtgagattt tagccatcaa ggttgcggac atcaatctgg
    18361 attttcaggt gctgcgaatc agcaaggctt cccaacagag gattgtcacc attcccacgg
    18421 ccttgctttc agaattggaa cccttgatgg ggcagaccta tctttttgaa agaggagaga
    18481 aaccctattc tcgtcagtgg gcctttcgtc agttagaatc ttttgtcaag gagaaaggtt
    18541 ttccatcctt atcagctcaa gtcttacgtg aacagtttat tctaagacaa atagaaaaca
    18601 aggtcgattt gtacgaaatt gcaaaaaaat taggattaaa aacagtcctg accttagaaa
    18661 aatatagata atggatatta aattaaaaga ttttgaagga cccctggact tgctcttgca
    18721 tctggtttct aagtaccaga tggatatcta cgatgtgccc attacggaag tcatcgaaca
    18781 gtatctagcc tatgtctcaa ccctgcaggc catgcgtctg gaagtgacgg gtgagtacat
    18841 ggtcatggct agtcagctca tgctgattaa gagtcgtaaa ctccttccga aggtagcaga
    18901 agtgacagac ttgggggatg acctggagca ggacctcctc tctcaaatcg aagaatatcg
    18961 caagttcaag ctcttgggtg agcacttgga agccaagcac caagaacggg cccagtatta
    19021 ttccaaagcg ccgacagagt tgatttacga agatgcggag cttgtgcatg acaagacgac
    19081 cattgacctc tttttggctt tttcaaatat cctagccaag aaaaaagagg agtttgcaca
    19141 aaatcacacg acgatcttgc gggatgagta taagattgag gacatgatga ttatcgtgaa
    19201 agagtccttg attggacgag atcaattgcg cttgcaggat ttgttcaagg aagcccagaa
    19261 tgtccaagag gtcatcaccc tctttttggc aaccctagag ttaatcaaaa cccaggagct
    19321 gatcctcgtg caagaggaga gttttggaga tatctatctc atggaaaaga aggaagaaag
    19381 tcaagtgcct caaagctaga cttgatagag aggaaagatg agtactttag caaaaataga
    19441 agcgctcttg tttgtagcgg gtgaagatgg gattcgggtc cgccagttag ctgaactcct
    19501 ctctctgcca ccgacaggca tccagcaaag tttaggaaaa ttagcccaga agtatgaaaa
    19561 ggacccagat tccagtttgg ctttgattga gacaagtggt gcttatagat tggtgaccaa
    19621 gcctcaattt gcagagattt tgaaggaata ctctaaggcg cctatcaacc agagcttgtc
    19681 tcgggctgcc cttgagacct tgtccattat tgcctacaaa cagccgatta cgcggataga
    19741 aattgatgcc atccgtggag ttaactcgag tggagccttg gcaaagttgc aggcttttga
    19801 cctgataaag gaagacggga aaaaggaagt attggggcgc cccaacctct atgtgactac
    19861 ggattatttc ctagattaca tggggataaa ccatttagaa gaattaccag tgattgatga
    19921 gcttgagatt caagcccaag aaagccaatt atttggtgaa aggatagaag aagatgagaa
    19981 tcaataagta tattgcccac gcaggtgtgg ccagtaggag aaaagcagaa gagctgatta
    20041 agcaaggctt ggtgacggtt aacggccaag tggtgcgtga actagcaacc actatcaagt
    20101 caggcgacaa ggtcgaagtt gaaggtcaac ctatctacaa cgaagaaaag gtctactatc
    20161 tgcttaacaa accacgcggt gtgatttcca gtgtgacaga tgataagggt cgcaagacgg
    20221 ttgtcgacct cttgcccaat gtcaaagagc gtatttaccc tgtgggtcgt ttggactggg
    20281 atacatcagg tgtcttgatt ttgaccaatg atggggactt tacagacgag atgattcacc
    20341 ctcgtaatga gattgacaag gtttatgtcg cgcgtgttaa aggtgtggcc aataaggaca
    20401 atctccgccc cttgacccgt ggtcttgaga ttgatggtaa gaaaaccaag ccagctgttt
    20461 atgaaattct caaagtggac ccagtcaaaa atcgctctgt ggtgcagttg accatccatg
    20521 aagggcgtaa ccatcaggtt aaaaagatgt ttgaagctgt tggtctccaa gtagataagt
    20581 tgtctcggac tcgtttcgga cacctagact tgacaggact ccgtccagga gaatcccgtc
    20641 gtcttaataa aaaagaaatc agccaactac acaccatggc tgtaactaag aaataatgaa
    20701 acgaatttta atagcgcctg tgcgctttta ccaacgtttt atctcaccag tctttccacc
    20761 ctcttgtcgc tttgagctga cttgttccaa ctacatgatt caggctattg aaaaacatgg
    20821 ttttaagggg gtattgatgg gcttggctcg gattttacgt tgtcatccct ggtcgaaaac
    20881 aggtaaggac cccgttccag accacttttc ccttaaacga aatcaagaag gggaatgagg
    20941 tggggtaaat agatttcaaa atgataaaaa cgcatcctat caggtttgag taaacttgat
    21001 aggatgcgtt ttagaatgtc aaaattttat actcttcgaa aatctcttca aaccgcgtca
    21061 gctttcatct gcaacctcaa aacagtgttt tgagcaacct gcggctagtt tcctagtttg
    21121 ctctttgatt ttcattgagt attaaattga gtttgaagtg gcttatttca aagctttttg
    21181 tatgtcttca atcatgagtt ttgttgattc aagtccgcct ccgcttagat accagaggtc
    21241 tggtgttagt tggataatct taccattttt agcagcaggt gtttcagcga taagggcatt
    21301 ttctaggaca ccgtcgttgc tagagttgtc cccaccgatg gcaagggtac ggttgatgac
    21361 aaagaggatg tcagggttga tttctttgac actttcaaag ctgacttctt gtccgtggcg
    21421 tgagtcttca aattttgtat cagttggttt gaatttcaag gtttggtaca agaaagagaa
    21481 acgagatttg gcaccaaagg ctgccatttt tccttcatta aggaggatcg caagggcttt
    21541 tttgtcagag ctttcatttt tagtagcgac ttcttggatg ctcttgtcta gcttggccaa
    21601 ttcttccttg gctttctgtg taccagtttc gccgaaggca cttgctaagg attcgatatt
    21661 agccttggta gaagtccagt agtcgtcctt gcttgcttgg aagagaacgg ttggggcgat
    21721 ttctttgaat ttgtctacga atttttgtgt acgtggtgaa gcgataatca aatcaggctc
    21781 aagggcggcg atagcttcta aatcaggttc tttcatagaa ccaacatttt tgacagttcc
    21841 cactaggtct tttagataag tcggaacagt ttttgtaggc attccgacga tatttttttc
    21901 aaatcctaaa gcgcgaatag tatccgcagc gccgaggtca aaggtcacaa tcttttcagg
    21961 aactttggaa agtttgacct cggccagtga acttttaatg gttacctctg ttggagcaga
    22021 gctactggtc tctgtctgac tagtgcttga gtttgtacta catgcaccaa gtaggagcaa
    22081 gaagctggcc actagggcag tgaaataaag tttaagggat gttttcataa tttctcctct
    22141 ttaaaatgtg ataacgattt agggagtctc ttgatcttat tgactaagag actgaaggtt
    22201 ctctaacttg agcttttatg ttactagcta tagatacaga tctttttgtc attgatatca
    22261 gctagcgtga tgggaatctc ataaagttga ctgagcaggt cagcctgcat gatttgatcg
    22321 gttcttccct tgctaaagac ctggccgtcc ttgaaggcga caatttcatc tgcatactga
    22381 ctggctatgt tgatatcgtg gaggacgatg ataatggtct tgccgagttc ctccaccagt
    22441 cgtcgaagaa tctgcatcat gctgacgctt tgcttgatat cgagattgtt gagtggttcg
    22501 tccagcaaga taaagtccgt atcctgggcc agtaccatag cgataaagac gcgctggagt
    22561 tgcccccctg acaggctatt gatgtagcgg tcttttaagt tggtcagttc caaatagttc
    22621 agagtttctc ggattttttc ccagtcttct gatctaagtc gacctcggct gtagggaaaa
    22681 cgtccaaaac tgaccagttc ttcaacagtc aatttggctt ggtaattgat tttctgtttt
    22741 aggatggtta gttcttgggc cagttcttgc gaattccagc tctcgatttc acgtcctttg
    22801 atactgagaa ctccctgatc tttcttggtt agtctgctca tgatggagag gagagtcgat
    22861 tttccagcac catttggacc aataaaggct gtcagttttt gaggactgac ttcaagcgaa
    22921 atgccttgca aaatatcctg tttttgaatg gatttgtcaa tgttttccag tttcactgac
    22981 gagccctcct atatagtaag ataaagaata agaagccacc cacactctca atgatcatac
    23041 tgatacgaat ttccagtgca aagactcgtt caatcaaggc ttgccccaag gttaagctaa
    23101 taaatccaac cagaatggcc actataaaga gtaacttgtg ctgatagtct ttgacaatca
    23161 ggtaggtgag gttggccagc ataaagccga agaaggccat aggtcctacc aaggaagtgg
    23221 ccgttgaggt caaaagcacg attccccaga ggagctcttt ctgttctttt tcaacatcga
    23281 gtcccaatat ctgagccgtt tctctttgca ggtgcaagac atctagaacg actgcttttc
    23341 gaaagaaaaa gattgtcaaa gcgaggatga tcagagaacc gatggctagg atggaagtgt
    23401 tgagatgttg aaaggaggca aaaagactat tttgcagttt atcgtattcg tttggatcca
    23461 ttaggacttg aaggaaggtg ctgatatttc gaaagagact tctgagcgct agacagatca
    23521 gcaggatgaa gaccaggtct tgcttcatca gtgtcttcaa gtaaccttgt aaggcgagaa
    23581 agaagaggga ctggacaaga agtaagacta ggaattctaa gataggggat ttgccaagtt
    23641 gaagaaactt gctttcaaaa accagtagta gggtttgtag taggacgtag aaggattcaa
    23701 ttcccaaaat actaggcgtc aggaagcgat tttccgtcag ggtttgaaaa ctaatggtcg
    23761 aaatcccagt cgcgatggct accaagagat aaacgatgat cttttgggaa cgcaacttcc
    23821 aagcaaaggc tgacaagtga gtgatgggcc aaaagtagag aagacaagct ccgatggcaa
    23881 gaataatgag aatccagaag agcttggtat gtttgctttt agtctgcatc ttttcgtccc
    23941 cctctccaga gaagtaggat aaagacgaga ctaccgatga ttcctagcaa gagactgaca
    24001 gacaactcat agggcctaat cagaactcgg gataggatat cgcaagccag aactagattg
    24061 gcaccaacca gtgcgaccat gagtttggtt tgacttagat tatctccata gcgcttgcga
    24121 acaagattgg gaacgataac tccgagaaat ggtaggccac ccacggtaat catggtgacg
    24181 cttgtcgtta gcgccaccag aaagagggcc agtttttcaa gtagggagta ggaaatcccc
    24241 aaactctcgc tggtttcttt ccctagattc atgatggtga aggtttggga taatttccaa
    24301 acggttatca ggatgatgag gcctaagaag agccactcat actgatgggt ctgaatcatg
    24361 gagaaggagc cctgggtcca ggcagtcata ctctgaacca gattgaaacg ataggcgata
    24421 acttctgtga ctgagccgat aatcccgcta tagatgatcc caatcagagg caacatccac
    24481 ctttccttta cagtaaaaat ggtcataaag gctaggaaga agagggtgaa tacgatggat
    24541 gaaacaaaag cgaagagcat cttttgggtc agactagccg atggaaagac aaaaaggctc
    24601 agcaccattc ccagtttggc ggcttcagtc gttccaactg tactcggtgc agcaaactga
    24661 ttttgggtaa tagtctgcat gagaaggcct gccatactca tactagaggc agtcaggaga
    24721 atactgatag ttcttgggag acgggactct tgaaagagga gccaggtctg ctggtcgaaa
    24781 tcaaatagct ttccccatga aaaatcactg gtcccaatgc taatagagag aaagactagg
    24841 agtagaagta agccaattaa ataatgagaa agtttcatac cccgtccttt catgtagatt
    24901 tggtatcgaa agatatctgc ggatataaat gtaacattat ttttctaatc tgtcaataaa
    24961 atttctgaca atttaataaa tacaacaagg agagagaaac aagactttct cctttgttat
    25021 cctattctaa aatgttttta ccttaatctg ataaaataat atcttcgagg gagtagctag
    25081 ccgtccaatc aagatattgt ttagcttttg aagcatctgc taggacactg gctgggtcac
    25141 tagcacgtcg agcaacaatc tcgtgtggga ttttttaatt tagtaattct tcagcagttt
    25201 taaagatttc tttgatagta tagccttttt tagttcctaa gttaaagatt tgagaagaac
    25261 tgtcttcttg aaataggtag ttcattcctt taacatgagc ctatgcaagg tccaagacat
    25321 aaatgtaatc tcgaatacat gaaccgtcac gtgtatcgta gtcatctcca aatattttta
    25381 agctatcatt ttgtcccaat gcggtcttgt tgatatttgg aatgatgtga gttggatttt
    25441 tcacacgcag accgtttgaa gcatccattt cagccccagc aacattaaag taacggaaaa
    25501 taacatattt ccagtcgtag cgattggcca tccagtaaat cattcgttcg cccatcagtt
    25561 ttgtctctgc ataagggttg acagggtcga gcagggtatc ttcagtcacc ggcttgtcaa
    25621 tacagttatt tccatagaga gaagcagtcg aagagaacat gattttttga atgccaactt
    25681 cagataagac tttgagaact tggttcatac cagcaacgtt ggcagtgaag tatttacttg
    25741 gattttcaat actttcgctc acaacaattt cacctgcaca atggaggaca gcatcaatct
    25801 gattttcttc taagtaagcc tttaaggcgc tagcatcata aacatccagt tctttaaagc
    25861 tagcacgact atccacagcc gctcgatttc ctgtggagag attatcgagg acatgcacct
    25921 gatagccagc atttaaaaga gctttaacgg tatgggagcc aatgtagcta gtcccacctg
    25981 taacaagaat tgttttggtc atgatttttt ccttttctag ttttgtacta tagtaaattg
    26041 aaataagata tgaacaactc tattaggaaa gtcaaattaa tttctagaaa tgtttcagca
    26101 gctacagcgt actattccaa actcaaccaa ctatatattt aaagaaagaa gaaagggaag
    26161 tcaactattt tctgtaaatt tcatgaaaaa tccaaacaac tttgatggtg tattgttttt
    26221 ttgtttggaa ataaatattc gagttaattt tggaatgttt actagatttt gcactacatg
    26281 caagagaata ctatcctttt ctaagataga gcaggactaa gatactaact tagaaatcca
    26341 ccttcccact atccttctcc tataaaaagt ggtaaaatgg atgaaagaaa gaaggaactg
    26401 aaatgacaac attattttca aaaattaaag aagtaacaga acttgctgca gtctcaggtc
    26461 atgaagcgcc tgtccgtgct tatcttcgtg aaaagttgac accgcatgtg gatgaagtgg
    26521 tgacagatgg cttgggtggt atttttggta tcaaacattc agaagctgtg gatgcaccgc
    26581 gcgtcttggt cgcttctcat atggacgaag ttggttttat ggtcagcgaa atcaagccag
    26641 atggtacctt ccgtgtcgta gaaatcggtg gctggaaccc catggtggtt agcagccaac
    26701 gtttcaaact cttgactcgt gatggtcatg aaattcctgt gatttcaggt tctgttcctc
    26761 cgcatttgac tcgtggaaag gggggaccaa ccatgccagc cattgccgat atcgtttttg
    26821 atggtggttt tgcggacaag gctgaggcag aaagttttgg catccgtcct ggtgatacca
    26881 tcgtaccaga tagttctgca attttgacag ccaatgaaaa aaatatcatc tcaaaagctt
    26941 gggataaccg ctacggtgtc ctcatggtaa gcgagctagc tgaagcttta tcaggtcaaa
    27001 aactcggcaa tgaactctat ctgggttcta acgtccaaga agaagttggt ctgcgtggcg
    27061 ctcatacctc tacaaccaag tttgacccag aagtcttcct cgcagttgat tgctcaccag
    27121 caggtgatgt ctacggtggt caaggcaaga ttggagatgg aaccttgatt cgtttctatg
    27181 atccaggtca cttgcttctc ccagggatga aggatttcct tttgacaacg gctgaagaag
    27241 ctggtatcaa gtaccaatac tactgtggta aaggcggaac agatgcaggt gcagctcatc
    27301 tgaaaaatgg tggtgtccca tcaacaacta tcggtgtctg cgctcgttat atccattctc
    27361 accaaaccct ctatgcaatg gatgacttcc tagaagcgca agctttctta caagccttgg
    27421 tgaagaaatt ggatcgttca acggttgatt tgattaaaca ttattaaacc ataagggagg
    27481 tggtagggat ggtagaacca aacctagaaa gccttataaa agatctttac aatcatgctc
    27541 gacatgattt gagtgaagat ttagttgctg ctctcctaga gactactaaa aaactgccta
    27601 ctacaaatga gcaattgcag gcagttcgtc tctcaagcct ggtcaatcgt gaattgctcc
    27661 taaatcccaa acatccagca cctgagttgc tcaacttggc tcgctttgtc aaaagagaag
    27721 aagccaagta cagaggaact gcgacttctg cgcttatgta tgaggaactt tttaaaatgc
    27781 tttgattcca ttgtggatca aagcattttt tataagacct atctcgtgag cgttcaaata
    27841 gtagtaacaa ggaatcatga gaagtcagcc gagaccatag tagtgaagaa acttctgtaa
    27901 tggaagtgga gtgaagtgag gaatcgtcaa acggtagggg ataggctgtt tgaagggtgc
    27961 tacgaaaaag atagggtaag gtgaaattat agcggaatga aataagatat gaacaaattg
    28021 attagggaaa tcaaatgaat ttatagaaat cttttagcag tcatggcgtc ctattctagt
    28081 ttcaaaatgc tataaatacg aacaaattga ttgggaaatt caaatcgctt tctatcaatg
    28141 ttttagaaga tacagtgccc tagtccaact tcaatatact ataaaagaac agatggaagc
    28201 aattatgatt tacttctatc tttaaaataa atgatctgat aggaaaaatg tgtcccctca
    28261 taaaaaatag gatagtacgg atggaaggct tttgtagact ggaggacgaa ggaatttaga
    28321 aactctcgtt aaaagttagt tgagccggag tatggtggtg tgaataatta tcaaaaatta
    28381 tttccaaatc gaaatagttg acagttagaa cgatgagtgt tacaattatt ttattcattt
    28441 cgatatcgaa ataaattgga ggtgttatga aagatagtca tttgctagcc catcatattc
    28501 gtttgttgaa tgggcggatt tttcaaaagt tactgagtca agatcctgag gctctttata
    28561 ggggtgaaca gggcaagatt ttagcggttt tatggaatag tgaaactggc tgcgcaactg
    28621 cgacagatat cgcgcttgcg actgggcttg cgaataatac gctgacgact atgataaaaa
    28681 agctagagga acaaaagctt gtaattgtta gtccgtgtgg aaaagacaag cgtaagaagt
    28741 atttagtttt aacggagtta ggcaagttcc agaaagaagt ggggcatcgt gtcagtcaga
    28801 aattggatac tatcttttac aaaggatttt cagaggaaga aattcaccaa tttgaaggtt
    28861 ttcaagaaag aattttggca aatctgaaag agaaggaaaa tgaggtttag aatatgtcac
    28921 aacaagtgaa aaatgctcac aacctataca tccatgccat tcaagacgga cgagttgctg
    28981 aggctcaagc ccagtctgta ggggatacct atatccaaca ttcgacaggt gttcctgacg
    29041 ggaaagaagg atttgcagcc ttctttgcag atttctttga gcgtcatcca gagcgtcaga
    29101 ttaagattgt ccgcaccatt gaggatggca atctggtctt tgttcatgtt catcaatatc
    29161 tgaatggtgg agaagctcaa tgggtgacga cggatacttt ccgtgcggat gagaatggtt
    29221 gtattgttga gcattgggat gtcattgact actatcgaac acctgaaaat gatcaactag
    29281 atcaaatttt tggagatttt gaaatcaagg atttggataa gaaagcagaa aataaaaagc
    29341 tggttcgccg tttcttgaca gaaattttcc aaaatgggga gctagagcag tggagtgatt
    29401 atgtggcaga cgatttgatt cagcataatc atgagattgg acaaggaagt gctgcttata
    29461 aaaactatgt ggctgaatat agtgtcactt ttgactttgt tttccaactc ttaggacaag
    29521 gaaactatgt ggttagctat ggtcagactc agattgatgg cgttgcttat gcccagtacg
    29581 atatcttccg tttagagaac gggaaaattg tggagcattg ggataataag gaagtcatgc
    29641 ctaaggtaga agacttgacc aatcgaggga agttttaaat tgaggacaaa gaatgattga
    29701 atacaaaaat gtagcactgc gctacacaga aaaggatgtc ttgagagatg tcaacttaca
    29761 gattgaggat ggggaattta tggttttagt agggccttct ggatcaggta agacgaccat
    29821 gctcaagatg attaaccgtc ttttggaacc aactgatgga aatatttata tggatgggaa
    29881 gcgcatcaaa gactatgatg agcgtgaact tcgtctttct actggttatg ttttacaggc
    29941 tattgctctt tttccaaatc taacagttgc ggaaaatatt gctctcattc ctgaaatgaa
    30001 ggggtggagc aaggaagaaa ttacgaagaa aacagaagag cttttggcta aggttggttt
    30061 accagtagcc gagtatgggc atcgcttacc tagtgaatta tctggtggag aacagcaacg
    30121 ggtcggtatt gtccgagcta tgattggtca gcccaagatt ttcctcatgg atgaaccctt
    30181 ttcggccttg gatgctattt cgagaaaaca gttgcaggtt ctgacaaaag aattgcataa
    30241 agagtttggg atgacaacga tttttgtaac ccatgatacg gatgaagcct tgaagttggc
    30301 ggaccgtatt gctgtcttgc aggatggaga aattcgccag gtagcgaatc ccgagacaat
    30361 tttaaaagtg cctgcaacag actttgtagc agacttgttt ggaggtagtg ttcatgacta
    30421 atttaattgc aacttttcag gatcgtttta gtgattggtt gacagctcta tctcaacatt
    30481 tgcagttgtc acttttgacc ttgttactag ctattttgct tgcgattccc ttggctgttt
    30541 ttcttcgcta tcatgagaag ctggccgact gggtcttgca gattgccggt attttccaga
    30601 ccatcccgtc tctggccttg ttggggctct ttatcccttt gatgggaatt gggaccttgc
    30661 cggctttgac agctctagtg atttatgcga ttttccctat tttgcaaaat actatcactg
    30721 ggctgaaggg aattgatccg aacctgcaag aggctgggat tgcctttggg atgaccagat
    30781 gggaacgtct caagaaattt gaaattccac ttgccatgcc tgttatcatg tctggaattc
    30841 ggacggcagc ggtcttgatt atcggtacgg ctaccttggc gaccttgatt ggggcagggg
    30901 gactgggttc ctttatcctg ttgggaattg accgtaataa tgccagtctg attttgattg
    30961 gggcactttc ttctgcagtg ctagctattg cctttaactt cctactaaaa gtaatggaaa
    31021 aagcgaaatt gcggacgatt ttctctggtt ttgccttggt ggctttatta ctgggtctgt
    31081 cttatagtcc agcccttttg gctcaaaaag agaaggaaaa cttgattatt gctgggaaaa
    31141 taggcccaga accagaaatt ttggctaata tgtataaatt gctgattgaa gaaaatacca
    31201 gcatgactgc gactgttaaa ccgaattttg ggacgacaag tttcctttat gaagctctga
    31261 aaaaaggtga tattgacatt tatcctgaat ttaccggtac ggtgactgaa agtttgcttc
    31321 aaccatcacc caaggtgagt catgagccag agcaggttta tcaagtggcg cgtgatggca
    31381 ttgccaagca ggatcatcta gcctatctca aacctatgtc ttatcaaaat acctatgctg
    31441 tagctgttcc gaaaaagatt gctcaagaat atggcttgaa gaccatttcg gatttgaaaa
    31501 aagtggaagg gcagctgaag gcaggcttta cgcttgagtt taacgaccgt gaagatggaa
    31561 ataagggctt gcaatcaatg tatggtctca atctcaatgt ggcgactatg cagccagctc
    31621 ttcgttatca ggcaattcag tcaggggata ttcaaatcac ggatgcctat tcgactgatg
    31681 cggaattgga gcgttatgat ttacaggtct tggaagatga caagcaactc ttcccacctt
    31741 atcaaggggc tccactcatg aaagaagctc ttctcaagaa acatccagag ttggaaagag
    31801 ttcttaatac attggctggt aagattacag aaaaccagat gagccagctc aactaccaag
    31861 tcggtgttga aggcaagtca gcagagcaag tagccaagga gtttctccaa gaacaaggtt
    31921 tgttgaagaa atgatgggac agagacttta aacatactga actcaacttc cttaaacgac
    31981 tagataaaag aatgctcaga caatttcgtc tgggcttttt tgatgtgaga atgactgaat
    32041 gtcacttaaa atggaaagaa agagaaaatt ttcatgattt tctaaaattt tactgtaaag
    32101 acagttgact attcataaaa taggtgtata atattttgtg aactacttaa caaataagag
    32161 gtttcagctc atgtcgacta aggttagaaa tcttgtgtat aggcagttca ttatctaata
    32221 gagcgttgcg tccgaaagtc tatccagaca cggctcttta aaaacaaaag gagaaatgat
    32281 gcatacttat ttgcaaaaga aaattgaaaa tatcaaaaca accctaggtg aaatgtcagg
    32341 tggttaccgt cgtatggttg cggctatggc tgatttagga ttttcaggaa ctatgaaggc
    32401 tatctgggat gacctctttg cccatcgtag ttttgcccag tggatttatt tgctggtttt
    32461 aggaagtttt cctctctggc tggagttggt ttacgaacat cgtattgttg actggattgg
    32521 gatgatttgt agcttgacag ggattatctg tgtaatcttt gtatcggaag gtcgagcaag
    32581 taattatctt tttggcttga ttaactctgt tatttacctt attttggccc tacagaaagg
    32641 cttttatggt gaggtgctga cgacacttta cttcacagtc atgcagccaa ttggacttct
    32701 agtttggatt tatcaggcac agtttaagaa ggaaaagcag gagtttgtcg cgcgtaaact
    32761 ggacggcaag ggctggacaa agtatctttc cattagtgtg ctttggtggt tggcctttgg
    32821 cttcatttat cagtctattg gtgccaatcg tccctatcgt gattcaatca cagatgcaac
    32881 caatggggta gggcaaatcc tcatgacagc tgtttaccgt gaacagtgga tattctgggc
    32941 ggctaccaat gtcttttcaa tctatctctg gtggggagaa agcctgcaaa ttcaagggaa
    33001 atatctaatt tatctcatta acagtctagt tggttggtat caatggagca aggcagctaa
    33061 gcagaatact gatttactta actaggaaaa gatgtttgaa agtgctgttt tgagatttcg
    33121 attaaaacag atatagttga taatcaagga tttatagtat gaaaaagagg atcggcgggt
    33181 cctcttttgt tgttgaaaag ataaaaaact cagtaatcta gaaataagac aactgagctt
    33241 tactctatat tcaattttta ggaatgagaa ggtctagata aaattggaca acttcctggt
    33301 ctgtgaaatc ttgacctttt ttgagccacc aggtcaatgt ctcgataaag ttggacatga
    33361 ccaagtgttg gaggtaagaa gtaggcagat tagggtgggc ttcttttaaa ttatcagcta
    33421 gcacggaata gacatggtgt tctagctctt tatggagttg acggaggaag tagtcatttt
    33481 tggaaaatag cagactggtg atatggtctt ggtttttatg aaaatgcaga aagaggtggg
    33541 cgaggtagtc ctcggttgaa atggcttgct ctctttcaaa aagatgatgg aagaggtagc
    33601 ggcagagctg gtccagaaga agctccttac tctcatagtg acagtaaaag gtggatcgtc
    33661 ccacatctgc gagatcaatg atatcctgaa cagtagtggc ctcgtagccc ttagcattca
    33721 aaagttgtat aaaagcttga tagatggctt ttttggtttt gctgatacgg cggtcaatgt
    33781 tagtcatatg gacacttgag gcaaattgtt cagaactgaa taaagctgac gttttgcttc
    33841 tatcctttct ttgagtttta gtggataatg ataatgaaca aggtgttcat aaatctatta
    33901 taacaaagga atgagaaata tgaaggcaaa atatgctgtt tgggtggctt ttttcttaaa
    33961 tttgacttat gccattgttg agtttattgc aggtggagta tttggttcta gcgctgttct
    34021 tgctgactct gtgcatgact tgggagatgc gattgcaatt ggaatatcag cttttctaga
    34081 aacaatctcc aatcgtgaag aagacaatca gtacaccttg ggctataagc ggtttagcct
    34141 gctaggagcc ttggtaacag ctgtgattct cgtaacgggc tctgttctag tcattttgga
    34201 aaatgtcacg aagattttgc atccgcaacc agtcaatgat gaggggattc tctggttagg
    34261 aattattgcg attactatca atctgttagc gagtctggtg gttggtaagg gaaagacaaa
    34321 gaatgagtct attctgagtc tgcattttct ggaagatacg ctagggtggg tagctgttat
    34381 cctgatggcg attgttcttc gatttacgga ctggtatatc ctagatcctc ttttgtccct
    34441 tgtcatttct ttctttattc tttcaaaagc ccttccacgt ttttggtcta cactcaagat
    34501 tttcttggat gctgtgccag aaggtcttga tatcaagcaa gtaaagagtg gcctggagcg
    34561 attggacaat gtggccagcc ttaatcagct taatctctgg actatggatg ctttggaaaa
    34621 aaatgccatt gtccatgttt gtctaaaaga aatggaacat atggaaactt gtaaagagtc
    34681 tattcgaatt ttcctaaaag attgtggttt tcaaaatatt accattgaaa ttgatgctga
    34741 cctagaaact caccaaaccc ataagcgaaa ggtgtgtgac ttggaacgga gttatgagca
    34801 tcaacattag aaaaaagtga aaaatacttg ggtactatct tatttggaat agagtaattt
    34861 ctttattatt taaatatttc aaaaattggt aagagaagag cattgtataa actccagata
    34921 tatgattgtt aatgataaaa atttttcgat tagatacaaa atgcttgact tggagtcaac
    34981 tcaaagttat ataataagat aagtgagtta gaatagcgtg aattcagtga atgaaatgag
    35041 aggaggttag cgtgtgaata ttaaatctgc cagtgatttg ttgggaattt cagcggatac
    35101 gattcggtat tatgaacggg ttggtcttgt gccaccgatt actcgtactg ctactgggat
    35161 tcgtgatttt caagatcagg atatcgaagc gctggaattt attaagtgtt ttcgttcggc
    35221 gggtgtctct gtagatagtt tagttgacta tatgtcgctc taccaaaagg gagatgaaac
    35281 gagagaggag aggcttggta ttttagaaga ggaaaagcaa aaattagagg agcgcttgtc
    35341 tcagctacag acagctttaa atcgtttaaa tctcaaaatt aaactttata aggaaggaaa
    35401 attttaaatg aaatcagcag tatatacaaa ggcaggtcag gttggacttg ctagcattga
    35461 acgtccgcaa ataatagaag cggatgatgt gattattcgt gtggttcgtg cgtgcgtttg
    35521 tggttcagat ttatggaggt accgtaatcc agaaacgaaa gctggacata aaaatagtgg
    35581 acacgaagcg attgggattg ttgaagaagc tggggaagcc attacgacgg tgaaaccagg
    35641 tgattttgtg attgtccctt ttacacatgg atgtggtgag tgtgatgcct gtcttgctgg
    35701 atttgacggt tcttgcgaca atcatattgg caataatttg gggggtgatt ttcaggcaga
    35761 atatattcgc ttccactatg caaactgggc gctggttaaa atccctggtc aaccttctga
    35821 ctatacagaa gggatgctca agtccctttt gactcttgca gatgtcatgc cgacaggcta
    35881 tcatgcggcg cgtgttgcaa atgttcaaaa aggggacaag gttgttgtta tcggtgatgg
    35941 ggctgttggt caatgtgctg tcatcgcggc taagatgcgt ggagcatcac aaattatcct
    36001 tatgagccgt catgaagacc gtcaaaagat ggctatggag tcaggtgcga cagctgttgt
    36061 tgcagaacgt ggtcaagaag gaattaccaa ggtgcgtgaa atcctcggtg gaggagcaga
    36121 tgcagcattt gaatgtgttg gtacggaggc tgctatagaa caggcgctag gtgttcttca
    36181 taatggaggg cgtatgggct ttgtaggagt cccacactat aataatcgtg ctcttggttc
    36241 gacatttatg caaaatatct ctgtagcagg tggggcagct tctgctacaa catacgataa
    36301 gcaattttta ctaaaagccg tccttgatgg tgatatcaat ccaggtcgcg tctttacttc
    36361 aagttataaa ctggaagata tcgaccaagc ctacaaagat atggatgaac gtaagaccat
    36421 taagtctatg attgtaatcg aataaaaaac gaataggagt tctaaaactc ctattcgttt
    36481 tttatgttat cctattcttg atttagggta ctttctctta atgtcagtct ggttcccagc
    36541 atggtcaggc tagggatttt ccgaccgtgg aggacttcct tgttaagaat atccatacct
    36601 gctcggccca tttcttcagt ataaactgta atactagaga ggggaggata gacctgtttg
    36661 gtcagactag tgtcgttaaa ggaaatgagg ctgacgcgat ctggcaggtt gattccagct
    36721 tcttggaggg cacggagggc accgatagct aaactatcgc tggctgcgaa aaatgctggc
    36781 ggaagttggt ctcccaagct ctgaatggcc tccttcatta agtcatagcc agactgggca
    36841 gtaaatcttc cttgaaagac cagttcatca tgatagattc ccctcgcttg actgtagttt
    36901 ctgaaatttt ccagccgctt atcctcaatg atttcttctt ggtctgttgt ttcttcaagg
    36961 cctgttagaa tcccgatacg gtccattcct tggctgagga aataatcgac aacctgtttc
    37021 atagcagtgt aaaaatccgt gataatacag gtatgtccca gggaaagtgt atcgctgtct
    37081 agaaatacaa gaggcttttg gtactcttca aaggcagaaa tctgagctcg actaaacttt
    37141 ccgatgcaga gaatcccaat cacttcctcg cttagggtaa aagggtggtc attaaaatag
    37201 cgcaagatat cgtagtccaa ctcttgggct cttttttcta ttcctaggcg aatctggtag
    37261 tagtagaggt cgtctagctc cccttgttcg ctgacccatt gaataatggc aatcttttgc
    37321 ttgggtttat gggactcgcc tgtcttgagg tgcttggtgt agcccagctc ttcagcaacg
    37381 gttaaaatac ggtgtctggt ttcttctgta acagataggc tctggtcgcg gttgagaaca
    37441 cgggatacgg tcgcgataga gacagaggct agctgtgcaa tgtcttttaa ggtagccata
    37501 aatcctcctt gattaggtta gtatatcata tttttcttct ttttactgat agtttagtaa
    37561 aattttagta aaaaggattg accttgggaa atcccttgga tacaatagaa gaaaacgatt
    37621 acacgttaag gtggcttaac ggacagtcaa aggagaaatc atatgacaca acatcttact
    37681 gctgaaactc ttcgcaaaga ctttcttgct gtttttggtc aagaagcaga ccaaaccttc
    37741 ttttcaccag gtcgtatcaa tttgattggt gaacacacag actacaacgg tgggcacgtt
    37801 tttcctgctg ctatttcctt gggaacttac ggtgcagctc gtaagcgtga cgaccaagtc
    37861 ttgcgtttct actcagctaa ctttgaggac aagggcatta tcgaagtgcc tctcgctgac
    37921 ctcaagtttg aaaaagagca caactggacc aattatccaa aaggtgtcct tcatttcttg
    37981 caagaagctg ggcacgtgat tgacaaaggt tttgattttt atgtttatgg gaatatccca
    38041 aatggtgctg gcttgtcttc ttcagcatcc ttggagctct tgacaggagt cgtggcagag
    38101 catctctttg atttaaaatt agagcgtctc gatttggtta aaatcggaaa acaaacagaa
    38161 aacaacttta tcggagtcaa ctctggtatc atggaccagt ttgctattgg tatgggggcc
    38221 gatcaacgtg ctatttacct tgataccaac accttagagt atgatttggt gccacttgat
    38281 ttgaaggaca atgtcgttgt tatcatgaac accaacaaac gccgtgaatt ggcggactct
    38341 aaatacaatg aacgtcgtgc tgagtgtgaa aaagcagtgg aagaattgca agttgccttg
    38401 gatattcaga ccctgggtga attggacgag tgggccgttg accaatatag ctatttgatt
    38461 aaagatgaaa atcgtttgaa acgtgctcgc catgctgtgc ttgaaaacca acgtaccctt
    38521 aaagctcaag cagcccttca agcaggtgat ttggaaacat ttggtcgttt gatgaatgcg
    38581 tcacacgttt ctctggagca tgattatgaa gtaactggtt tggaattgga tactcttgtt
    38641 cacacagctt gggcacaaga aggagttctc ggtgctcgta tgacaggggc tggttttggt
    38701 ggctgtgcca ttgccttggt tcaaaaagat actgttgagg cctttaagga aactgtaggc
    38761 aaacactacg aggaagtagt tggatacgct ccaagcttct atatcgctga agttgcaggt
    38821 ggcactcacg tccttgacta gtcaaaagga ggctctatgg tgaccttagt agataaattt
    38881 gtaacacatg tcatttttga aagttcattt gaggaaatgg atcgaatcta cctgaccaac
    38941 cgtgttttgg cacgagtggg agaaggtgtt ttggaagttg agaccaatct ggataaattg
    39001 attgacctca aggaccagct agttgaggaa gccgttcgat tagagatgat tgaggatagt
    39061 cagactgcgc gtgaaatcct tggtactgaa ctgatggact tggtgactcc ttatccgagt
    39121 caggtcaatc gtgacttctg ggaagcctat gtccactctc ctgagcaagc gatagaggat
    39181 ttttatcaac tcagtcagaa aaatgattac atcaaactca aggccattgc taaaaatatc
    39241 gcttatcgtg ttccatctga ttatggagaa cttgaaatta ccatcaatct ctctaagcct
    39301 gaaaaggatc cgaaagagat tgcggtagcc aagttggtgc aagctagtaa ttatcctcag
    39361 tgtcaacttt gtttagagaa tgagggttac catggtcggg tcaaccaccc agctcgcagc
    39421 aaccaccgga ttatccgttt tgaaatggtt ggtcaggaat ggggtttcca gtattcgccc
    39481 tatgcttact ttaatgagca ttgtatcttt ttagatggcc agcatcgtcc catggccatt
    39541 agtcgtcaga gttttgaacg tctgttggct atcgtagagc agtttccagg atattttgct
    39601 ggatctaatg ccgacctgcc gattgtgggg ggctctattc taactcatga tcattatcag
    39661 ggaggccgtc acgtatttcc tatggaattg gctcccttgc aaaagacctt ccgatttgct
    39721 ggttttgagc aggtcaaggc tggaattatc aagtggccaa tgtctgtcct acgcttgact
    39781 tcggattcca aagaggattt gattaactta gctgataaga ttttccagga atggcgtcag
    39841 tattcagatt ctagtgtgca gattttagca gagacagacg ggacaccgca tcacaccatc
    39901 acaccaatag cgcgtaagcg tgatggacag tttgagttgg acttggtctt gcgagacaat
    39961 cagacttcag cagagcatcc tgatggcatc tatcatcccc acaaggatgt ccaacatatc
    40021 aagaaggaaa atatcggctt gattgaggtc atgggcttgg caatcttgcc accacgtctg
    40081 aaagaagaag tagagcaagt cgctagctat cttgtaggag aagctgttac agttgccgat
    40141 tatcatcagg agtgggcaga ccaactcaaa tcccaacatc cagacctaac ggataaagaa
    40201 aaagcccttg caatcgtcaa ggactctgtg ggtgctatct ttgtgcgtgt acttgaggat
    40261 gcaggagtct acaagcagac agaacaaggg cagacagcct ttatgcgctt tgtggaacag
    40321 gtcggaattt tactagacta ggagctttct ccttgcacag tcctataaaa agtagtatca
    40381 tagtgtcgtt tgaaatatca ggaggaaacg atgaaagact ttcattttga cgctatatct
    40441 gcctttgaaa attacgaaat tgaaaaaatg agagatggtc acgttgtggt gacgaccaaa
    40501 gtagtgaact catcgctcaa ctactatggc aatgcccatg gtggctatct attcacgcta
    40561 tgtgaccaaa tcagtggttt ggtggttatc tcgctgggac ttgatggggt gacactccaa
    40621 tcctctatca actaccttaa ggcaggaaaa ctcgacgatg tattgaccat taaaggagaa
    40681 tgtgtccatc aaggtcgtac aacctgtgtg atggatgtgg atatcaccaa tcaagagggc
    40741 agaaatgtct gtaaggcaac ctttaccatg tttgtcacag gccaaaggtc tgaggaaaga
    40801 cgggtaagta tataaagaaa ggtgagtggg ataaatttat ctcactcatt tttaatttat
    40861 gagatatgtt agaaggaaat ataaaagaga gtgatgaaat acaaatgtaa taggtacata
    40921 tatgtggtat actagttata gtagatttta attgcttata tcgagtgagt tctcatatta
    40981 gtgaggaatt tttaaatgga attacacttt aatttagaat tagtagaaac ctataaaagt
    41041 aatagccaaa aggcacgtat cttaacggag gattgggttt atcgtcagtc ttattgccct
    41101 aattgtggga acaatccctt aaatcatttt gaaaataatc ggcctgtagc agatttttac
    41161 tgtaatcatt gtagtgagga gtttgaacta aagagcaaaa aaggaaattt ttcatcaaca
    41221 atcaatgatg gtgcttatgc aacgatgatg aagcgtgtgc aggcagataa taatcctaat
    41281 ttcttttttt taacttacac aaaaaatttt gaggtaaata actttcttgt ccttccgaag
    41341 caatttgtta caccgaaatc gattattcaa agaaaaccac ttgcaccaac tgctagacga
    41401 gcaggttgga ttggttgtaa cattgattta tcacaagtac cttctaaagg aaggatattt
    41461 cttgtgcaag atggacaagt tagagatcca gaaaaagtta caaaagaatt taagcaaggt
    41521 ttatttttaa ggaagagctc tctgtcatca agaggttgga caatagaaat tctaaattgt
    41581 atagataaga tagagggttc agaatttacc cttgaagata tgtatcgttt tgaaagtgac
    41641 ctaaaaaata tctttgttaa gaacaatcat atcaaagaaa agattaggca acagcttcaa
    41701 atattaagag acaaagaaat aatagaattt aaaggtagag gaaagtatcg gaaattatga
    41761 aaacgaaaca acttgttgca tcagaagagg tgtatgattt cttaaaagtc atctggcctg
    41821 attatgaaac tgaaagccgt tacgataacc taagtttaat cgtctgtacc ttatcagatc
    41881 ccgattgtgt gagatggtta tctgaaaata tgaaatttgg tgacgaaaaa caactagctt
    41941 tgatgaagga aaaatatggg tgggaagtag gagataaatt gccagagtgg ctacatagct
    42001 cctatcatag attattgtta ataggtgaat tattggaaag caatctaaaa ctgaaaaagt
    42061 atacagtaga aattacagaa actttatcac gtttagtaag tatagaggct gaaaatccag
    42121 atgaagccga acgacttgta agagaaaagt ataagagttg tgaaattgtt cttgatgcag
    42181 atgattttca ggactatgac actagcatat atgaataggt agatgttttt attttgtcac
    42241 aca
//
LOCUS       DAVLWA010000007        41701 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLWA010000007 DAVLWA010000000
VERSION     DAVLWA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.007"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            337..2490
                     /locus_tag="VYN80_000367"
     CDS             337..2490
                     /locus_tag="VYN80_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1540341.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2503..3852
                     /locus_tag="VYN80_000368"
     CDS             2503..3852
                     /locus_tag="VYN80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1540342.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4022..4729
                     /locus_tag="VYN80_000369"
     CDS             4022..4729
                     /locus_tag="VYN80_000369"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1540343.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4786..8511
                     /locus_tag="VYN80_000370"
     CDS             4786..8511
                     /locus_tag="VYN80_000370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1540344.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVVSDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8604..10046
                     /locus_tag="VYN80_000371"
     CDS             8604..10046
                     /locus_tag="VYN80_000371"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1540345.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10083..11105
                     /locus_tag="VYN80_000372"
     CDS             10083..11105
                     /locus_tag="VYN80_000372"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1540346.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11102..11647
                     /gene="purN"
                     /locus_tag="VYN80_000373"
     CDS             11102..11647
                     /gene="purN"
                     /locus_tag="VYN80_000373"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1540347.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11731..12240
                     /locus_tag="VYN80_000374"
     CDS             11731..12240
                     /locus_tag="VYN80_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1540348.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12265..13812
                     /gene="purH"
                     /locus_tag="VYN80_000375"
     CDS             12265..13812
                     /gene="purH"
                     /locus_tag="VYN80_000375"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1540349.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQEFIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13934..15196
                     /gene="purD"
                     /locus_tag="VYN80_000376"
     CDS             13934..15196
                     /gene="purD"
                     /locus_tag="VYN80_000376"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1540350.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKANDIAWTFIGPDDALAAGIVDDFHAAGLKAFGPTRLAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15599..16087
                     /gene="purE"
                     /locus_tag="VYN80_000377"
     CDS             15599..16087
                     /gene="purE"
                     /locus_tag="VYN80_000377"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1540351.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16074..17147
                     /gene="purK"
                     /locus_tag="VYN80_000378"
     CDS             16074..17147
                     /gene="purK"
                     /locus_tag="VYN80_000378"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1540352.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVGEF"
     gene            17170..19557
                     /locus_tag="VYN80_000379"
     CDS             17170..19557
                     /locus_tag="VYN80_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1540353.1"
                     /translation="MFEKIKGINIKSGIFEDETKLELFEGNFEGTNPVQNDRASLLFG
                     RNGSGKSTIARGINQLKNGEIGTDRVSFIDKNNNNIVLSDTERKSIFVFNEHYVDQKV
                     KIAQEGLDTIVILGEQVDIDEELDRLRTQLSESQIESQDYYAEYEEYLDEKNEKSPDF
                     WKKEMTDSLKGVGNWAERDREIKGNRAASPVHNNTFQNFVDLQPILDKNELEVEFNNK
                     KARYFSIRDSAVTINNELSLPDINFDSNELSTLLSEKIEEPELNSRDKYLLTLLSDST
                     KGERHLREVKDFFEDEHQKKCPFCTQSVSEDVKVELTNGITKLLSRAVEEHQSALRGK
                     KIDEINQDFSGYEQIDPILIQSYQNSINALNAKFNEINSIIDKKIDNPYNIVELPNIS
                     FSQELSQAEHDIEKINQAIIKHNSEISGIQKLKVDLLQINNELAFYEIQDAYKKFQEK
                     TNKKAICENNYNNSSKRVKDYEKQISDLEDKKLNIDIAVDEINKSLNYIFFSKNRLAI
                     QNQNGKYYLLSRGKSVVLSRVSVGERNALALCYFFTEIIQQRELADAYSHEYFIVIDD
                     PISSFDMENKVGITSYLKYCLTRFFKGNSNTRVLLMTHDKQTIYDFDIFLKEIMESCK
                     EEEGGQKSKYKKLELVSGKLQEFKTSTHDYTELLEIVFGYALGNSTPTSESFVGNAMR
                     KILEAYGSFNYKKGIAELTTDPLIVEKIDKEYRTYFENLMYRLVLNGESHFKDPVKTL
                     SIDFFDTISDEERKKTARDLLVLLYLLDDLHVLKHLEGVSNAENRLEQWKCEILE"
     gene            19730..21028
                     /gene="purB"
                     /locus_tag="VYN80_000380"
     CDS             19730..21028
                     /gene="purB"
                     /locus_tag="VYN80_000380"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1540354.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEINEIFNPIYYTKRVDDIFERLGLGD"
     gene            complement(21083..25021)
                     /gene="strH"
                     /locus_tag="VYN80_000381"
     CDS             complement(21083..25021)
                     /gene="strH"
                     /locus_tag="VYN80_000381"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1540355.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPRLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(25336..26052)
                     /locus_tag="VYN80_000382"
     CDS             complement(25336..26052)
                     /locus_tag="VYN80_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1540356.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            26400..28187
                     /locus_tag="VYN80_000383"
     CDS             26400..28187
                     /locus_tag="VYN80_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1540357.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRIPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLNNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            28184..28660
                     /locus_tag="VYN80_000384"
     CDS             28184..28660
                     /locus_tag="VYN80_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1540358.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            28688..29593
                     /locus_tag="VYN80_000385"
     CDS             28688..29593
                     /locus_tag="VYN80_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1540359.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            29580..30395
                     /locus_tag="VYN80_000386"
     CDS             29580..30395
                     /locus_tag="VYN80_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1540360.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            30402..30806
                     /locus_tag="VYN80_000387"
     CDS             30402..30806
                     /locus_tag="VYN80_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1540361.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            31105..32271
                     /locus_tag="VYN80_000388"
     CDS             31105..32271
                     /locus_tag="VYN80_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1540362.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            32387..33424
                     /locus_tag="VYN80_000389"
     CDS             32387..33424
                     /locus_tag="VYN80_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1540363.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            33632..34486
                     /locus_tag="VYN80_000390"
     CDS             33632..34486
                     /locus_tag="VYN80_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1540364.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            34623..34880
                     /locus_tag="VYN80_000391"
     CDS             34623..34880
                     /locus_tag="VYN80_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1540365.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(34922..35284)
                     /locus_tag="VYN80_000392"
     CDS             complement(34922..35284)
                     /locus_tag="VYN80_000392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540366.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(35338..35634)
                     /locus_tag="VYN80_000393"
     CDS             complement(35338..35634)
                     /locus_tag="VYN80_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1540367.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(35624..35785)
                     /locus_tag="VYN80_000394"
     CDS             complement(35624..35785)
                     /locus_tag="VYN80_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1540368.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            35855..36619
                     /locus_tag="VYN80_000395"
     CDS             35855..36619
                     /locus_tag="VYN80_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1540369.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(36859..37011)
                     /locus_tag="VYN80_000396"
     CDS             complement(36859..37011)
                     /locus_tag="VYN80_000396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540370.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            37229..38608
                     /locus_tag="VYN80_000397"
     CDS             37229..38608
                     /locus_tag="VYN80_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1540371.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            38622..39287
                     /locus_tag="VYN80_000398"
     CDS             38622..39287
                     /locus_tag="VYN80_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1540372.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            39401..39496
                     /locus_tag="VYN80_000399"
                     /pseudo
     CDS             39401..39496
                     /locus_tag="VYN80_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            39642..40180
                     /locus_tag="VYN80_000400"
                     /pseudo
     CDS             39642..40180
                     /locus_tag="VYN80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            40247..40657
                     /locus_tag="VYN80_000401"
     CDS             40247..40657
                     /locus_tag="VYN80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1540373.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            40773..>41701
                     /gene="pavB"
                     /locus_tag="VYN80_000402"
     CDS             40773..>41701
                     /gene="pavB"
                     /locus_tag="VYN80_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1540374.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASLAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNV"
BASE COUNT        11750 a         7942 c         9614 g        12395 t
ORIGIN      
        1 atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt gttctgccta
       61 tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa ggaagtccag
      121 tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac tcttcgaaaa
      181 tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc
      241 tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta gtttgctctt
      301 tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa acgtcactat
      361 cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt ttttggctac
      421 tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac catggatggg
      481 acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac gcgagccatt
      541 aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt tgcccatgtg
      601 cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa ggatagcatt
      661 catattgccg atccagatcc cggggtgaag ttgactaaac tgccacgtga gcgttttgag
      721 gaagaatgga caggagtgac tctttttatg gcacctagtc cagactataa gcctcataag
      781 gaacaaaaaa atggtctgct ctcttttatc cctatattag tgaagcagcg tggcttgatt
      841 gccaatatcg ttttggcaac actcttggta accgtgatta acattgtggg ttcttattat
      901 ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact agggattatt
      961 tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc tcaggagtat
     1021 ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta tatcaagcat
     1081 gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat cgtgtctcgt
     1141 tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc gattttccta
     1201 gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac caatctcttt
     1261 ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt tatgaagccg
     1321 tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc ttctatcatt
     1381 gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca gcgttaccaa
     1441 aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag tcgagcagag
     1501 agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg cattctctgg
     1561 atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat tacctataat
     1621 accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca aaccaagctt
     1681 cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc ttctgagttt
     1741 gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac cttcaagcag
     1801 gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt aaccgttccc
     1861 caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac tttggccaag
     1921 atgatggtta atttttacga cccaagtcaa ggggagatta gtctgggtgg tgtcaatctc
     1981 aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca acagccctat
     2041 gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg gacgacacag
     2101 gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga gcgcatgcca
     2161 ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg tcaacgtcag
     2221 agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg
     2281 actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat tgctttggac
     2341 aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga gaaggtggtt
     2401 gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga
     2461 ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc agaattttta
     2521 gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt gattgtaccc
     2581 atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa ggagatgagt
     2641 ttgtccacta gagctactgt cgaacccagt cgtatccttg caaatatcca gtcaactagc
     2701 aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa gggggatctt
     2761 ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc cagtcagttg
     2821 gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct gcaagaaggg
     2881 gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg cgactacatc
     2941 agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag
     3001 aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca aacagaggct
     3061 aaaattcgcg attaccagac agctaagtca gctattgaaa caggtacttc cttggccggt
     3121 cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgagga aaatccccaa
     3181 actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga atctagtctt
     3241 gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc gtcagggtta
     3301 agcagtcaat tggaatccct taaatcccaa cacttggcaa aggttggtca ggaattgacc
     3361 cttctagccc agaaaatctt ggaggcagag tcaggtaaga aggtacaggg aaatctttta
     3421 gacaagggga aaattacggc aagtgaggat ggggtgcttc atcttaatcc tgagaccagt
     3481 gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc tttggaaaga
     3541 gaagggaaag ccaaactcac agcctatcta agttcaaaag atgtagcaag aatcaaggtc
     3601 ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt tctagattct
     3661 actattacaa gtattgatgc gacagctact aagactgaga aagggaattt ctttaaaatc
     3721 gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc
     3781 cgtctacaga tgattacggg caagaaaagt tatctacgtt attatttgga tcaatttttg
     3841 aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg agaaagattc
     3901 ttcttgcagt tttttcttta caatttttga aaaacatctg ctatttattc ggttaaattc
     3961 ttgtgttttt tggttttttg tggtaaaatg tgctcaagta atacgaaagg cgaactttaa
     4021 aatgtcaaaa caattgatct attcgggaaa agctaaagat atctatacaa ctgaggatga
     4081 aaatcttatt atttcaactt acaaggacca ggcgactgct ttcaatggtg tcaagaagga
     4141 gcagattgca ggtaagggag tcttgaataa tcagatttca tcttttattt ttgagaaatt
     4201 aaatgcggct ggtgtggcga ctcactttgt ggagaaactt tcagacacgg aacaactcaa
     4261 taaaaaggtt aagattattc ctttggaagt cgtgctccgc aactatactg ctggttcctt
     4321 ttcaaaacgt tttggtgtgg atgagggaat cgccttggag actccgattg tcgaatttta
     4381 ctacaaaaat gatgatttgg atgatccatt tatcaatgat gagcatgtga aattcctaca
     4441 gattgcggat gaccagcaga ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact
     4501 attgaaagtc tggtttgctg agattgggct taaattgatt gactttaagc tagagttcgg
     4561 ttttgacaag gatggcaaga ttatcttggc agacgaattt tcaccagata actgccgctt
     4621 gtgggatgct gatggcaacc acatggataa ggatgttttc cgtagaggat tgggagaact
     4681 aaccgacgtt tatgagattg tttgggaaaa gttgcagggt ttaaaataac aacctcaagg
     4741 ctgtttggga atattgcaag agctgaaata aaggaataag aattgatgga taaacgtatt
     4801 tttgttgaaa aaaaggctga ttttcaggtc aagtcagaga gtttggttag agagctccag
     4861 cacaacttgg gactgtcaag cttgaaaagt attcgtattg tgcaagtata tgatgtattt
     4921 gacttggttg aggacttgtt tgcacctgca gagaagcaca ttttctctga gcaggtaacc
     4981 gaccatgttt tagatgaagt atctgtgcag gcggatcttg ctaactatgc tttctttgcc
     5041 attgaaagtc taccagggca gtttgaccag cgtgcagctt cgtcacagga agccttgctt
     5101 ttgttgggaa gttcgagtga cgtgacagtc aacacagccc aactttactt ggtgaataaa
     5161 gatattgatg cgactgagtt ggaagctgtc aaaaactacc tgctcaatcc agttgattct
     5221 cgtttcaagg atatcacgat agggattgcc aagcaggagt tttcagagtc agacaagacc
     5281 attccaaaat tgcctttctt tgaaagctat acagcagaag actttgctcg ctacaaggcc
     5341 gaacaaggga tggccatgga agtggatgat ttgctcttta tccaagacta ctttaagtca
     5401 atcgggcgcg tgccaactga gactgaactc aaggttttgg acacttactg gtctgaccac
     5461 tgccgtcata cgacttttga gacagagttg aaacacatcg acttttcagc ttctaaattt
     5521 caaaagcaat tgcagtcaac ctatgacaag tatattgcca tgcgcgagga attaggtcgg
     5581 tctgaaaaac cacaaacctt gatggatatg gcgactattt tcggtcgtta tgagcgtgct
     5641 aatggacgat tggatgatat ggaagtctct gacgaaatca atgcctgctc agttgaaatt
     5701 gaagtggacg ttgatggtgt caaggaacct tggctcctca tgtttaaaaa cgaaacccac
     5761 aaccatccaa cagaaattga gccatttggt ggagcggcta cctgtatcgg tggagctatt
     5821 cgtgatccgt tgtcaggccg ttcctatgtt taccaagcca tgcgtatttc aggtgctggt
     5881 gatattacag caccgatttc ggaaactcgt gctgggaaat tgccacaaca agtcatttct
     5941 aaaacagcaa ctcatggtta ttcttcatat ggtaaccaga ttgggcttgc cacaacctac
     6001 gttcgtgaat acttccaccc aggctttgta gctaaacgta tggaacttgg tgccgttgtt
     6061 ggtgcggctc ccaagggcaa tgttgtccgt gaaaaacctg aagcaggtga tgtgatcatc
     6121 cttctcggag gtaaaacagg tcgtgatggt gtcggtggtg cgacgggctc ttctaaggtt
     6181 caaacagttg agtctgtaga gactgctggt gctgaggttc aaaaaggaaa tgccatcgaa
     6241 gaacgcaaga ttcagcgcct cttccgtaat ggcaatgtca ctcgtctgat caagaagtcc
     6301 aatgactttg gggcaggcgg tgtctgtgtg gctatcggtg aattggcaga cggtcttgaa
     6361 atcgacctca acaaggtgcc tcttaaatac cagggcttga atggtacaga aattgccatc
     6421 tctgaatcac aagaacggat ggcggtcgtg gttcgtcctg aagatgtgga taccttcgtt
     6481 gccgaatgta acaaagaaaa tattgatgct gttgtggtgg cgacagtaac tgaaaaacca
     6541 aatcttgtca tgcactggaa tggtgagaca atcgttgact tggagcgtcg tttccttgac
     6601 accaatggtg tgcgcgtggt tgtcgatgcc aaagttgtgg acaaggatgt caaactccca
     6661 gaagagcgtc aaacatctgc tgaaacactg gaatcagata cccttacggt tctatctgac
     6721 ctcaaccatg caagtcaaaa aggattacag actatctttg actgctctgt tggacgctca
     6781 acggttaatc acccacttgg tggtcgttac caactcacac caactgaggc atctgtgcag
     6841 aaattgccag ttcaacacgg tgtgactcat actgcgtcgg tcattgctca aggtttcaac
     6901 ccatatgtag ctgaatggtc tccataccac ggtgctgctt atgcggttat cgaagcaact
     6961 gctcgtttgg tggctgctgg tgccaactgg tctaaggctc gtttctctta ccaagaatat
     7021 ttcgagcgga tggacaagca agcagagcgt tttggtcagc cagtagccgc ccttctaggc
     7081 tctattgaag cacaaattca gcttggcttg ccatctatcg gtggtaagga ctccatgtct
     7141 ggtacctttg aagaattgac cgttccgcca accttggttg cctttggggt gacgacggca
     7201 gatagccgta aggtgctctc tccagaattt aaagctgttg gggaaaatat ctactacatc
     7261 ccaggtcaag ccctctctgc agagattgat tttgacttga ttaagaaaaa ttttgctcag
     7321 tttgaagcaa gccaagctga ccataaagtg acatctgcat cagctgtcaa atacggtggt
     7381 gtagttgaaa gtttggctct tgctaccttt ggaaactata ttggtgcaga ggtgaccttg
     7441 cctgaactta aaacagcttt gacagctcaa ttaggcggct ttgtcttcac atctcctgaa
     7501 gaaattgctg gagtagagaa gattggacaa acgaaagcag actttacact gactgtcaac
     7561 ggtgtgaagc tagatggaca caagcttgac agtgcatttc aagggacatt ggaagaagtt
     7621 tacccaacag aatttaccca agcgaaagaa ctagaagaag taccagctgt ggtatcagat
     7681 gttgtgatta aagccaaaga aaaggttgaa aaacctgtgg tttacatccc agtctttcca
     7741 ggaaccaact cagaatatga ttcagctaag gccttcgaaa aagaaggtgc agaggtcaat
     7801 ttggtgccat tcgtgacctt gaatgaagaa gctattgtca agtcagttga aactatggtt
     7861 gacaatatcg acaagactaa tattctcttc tttgccggtg gattctcggc tgcggatgaa
     7921 ccagatggtt cagctaagtt tatcgtcaat atcctgctta atgaaaaagt gcgtgtggct
     7981 attgatagct ttatcgcccg tggtggcttg attatcggta tttgtaatgg attccaagcc
     8041 ttagtcaaat cgggtctcct accatacgga aactttgaag atgctaacag tactagtcca
     8101 accctcttct acaatgatgc caaccaacac gtggccaaga tggtggaaac tcgtattgcc
     8161 aataccaact caccatggtt ggctggtgtg caagtgggcg atatccacgc tattcctgtt
     8221 tcgcacggtg aagggaagtt tgtcgtgacg gctgaggaat ttgcagagct ccgtgacaat
     8281 ggacaaattt tcagccaata cgttgacttt aacggtaaac caagtatgga ttctaagtac
     8341 aatccgaatg gttctgtcca tgccatcgaa ggaattacca gcaagaatgg tcaaatcatc
     8401 ggtaagatgg gccactcaga acgttatgag gatggtcttt tccaaaatat cccaggcaat
     8461 aaagaccaac acctgttcgc atcagcggtt aaacatttca ctggaaaata agacttacag
     8521 attttctaat agatagtatc agtaatgtaa aagtcatgta aatctagctc ttgatgatta
     8581 caaatgaaaa ttaggtataa aaaatgacat acgaagtaaa atctcttaat gaagaatgtg
     8641 gtgttttcgg tatttgggga catccagatg ctgctaagtt gacctatttt ggactccaca
     8701 gtcttcaaca ccgtggtcag gagggggcag gaatcctctc caatgatcaa ggacaactga
     8761 agcgccatcg tgacatgggg cttttatcag aagttttcag aaatccagcc aatttggata
     8821 aattgacagg agctggtgcg attgggcatg tgcgttatgc gactgctggc gaagcttctg
     8881 ttgataacat ccagcccttc ctcttccgtt ttcacgatat gcagtttggc ttggctcata
     8941 atggaaatct gaccaatgca gcctctctca agaaagaact ggaacaaaga ggagcaattt
     9001 tcagcgcgac ttcggactct gaaatcttgg ctcacctcat tcgtcgcagt cataatccta
     9061 gcctgatggg caaaatcaag gaagcgctca gccttgtcaa aggtggtttt gcctatatct
     9121 tgctgtttga ggacaagttg attgcggctc ttgaccctaa tggctttcgt ccgctttcta
     9181 tcgggaaaat ggccaacgga gcggtggttg tttcctctga aacctgtgct tttgaggtca
     9241 ttggtgccga gtggattcgt gatttgaagc caggtgagat tgtgatcatt gatgacgagg
     9301 gcattcagta tgacagctat acagatgata ctcagttggc gatttgttct atggagtata
     9361 tctactttgc tcgccctgat tctaatatcc acggtgtcaa tgtccatacg gcacgtaaga
     9421 gaatgggagc gcaattggcg cgagaattta agcatgaggc agatattgta gttggtgtgc
     9481 ccaattcttc cctaagcgcg gctatgggat ttgcggaaga atcaggctta ccaaatgaaa
     9541 tgggtctgat caaaaaccaa tacacccagc gaacttttat ccaaccgact caagaattgc
     9601 gggagcaagg agtgcggatg aaactgtctg ctgtttcggg tgttgtcaaa ggcaaacgtg
     9661 tggtcatggt ggatgattcc attgtacgtg gaacaacctc tcgtcgtatc gttcagctct
     9721 tgaaagaagc gggtgcgact gaggttcacg ttgccattgg aagtcctgca ctagcgtatc
     9781 catgtttcta cgggattgat atccagaccc gtcaggagct gattgcagcc aatcatacgg
     9841 tcgaagaaac tcgccaaatc attggtgcgg acagtctgac ttatctttca attgatggct
     9901 tgattgagtc gattggtatc gaaacagatg cgccgaacgg tggtctctgt gtcgcttact
     9961 ttgacggtga ctacccaacg cctctctatg actacgaaga agactatcgt agaagtttgg
    10021 aagaaaagac cagtttttac aagtaggcga cagattctcc attaaagaaa aggaaaaaac
    10081 aaatgacaaa taaaaatgca tatgcccagt cgggtgtgga tgttgaagcg ggttatgaag
    10141 ttgttgaacg gattaaaaag cacgtggccc gtacggagcg tgcaggtgtc atgggagctc
    10201 ttggtggctt tggtggtatg tttgaccttt ccaagactgg ggttaaagaa cccgtcttga
    10261 tttcagggac tgacggtgtc ggaaccaagc tcatgttggc tatcaagtac gacaagcacg
    10321 ataccatcgg gcaggactgt gtggccatgt gtgtcaacga catcattgct gcaggtgcgg
    10381 aacccctcta ttttctcgac tacgtagcga cagggaagaa tgaaccagct aagctagaac
    10441 aagtggttgc tggtgtggca gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg
    10501 aaacggctga aatgccgggc atgtacggcg aagacgacta tgacttggct ggttttgcgg
    10561 tcggtgtggc tgaaaaatct caaatcattg acggttcaaa ggtggtagag ggagatgttc
    10621 ttctcggact tgcttcaagt gggattcact caaatggtta ctctttggtt cgtcgtgtct
    10681 ttgcggatta cacaggtgag gaagtcctac cagaattgga aggcaagaaa cttaaggaag
    10741 ttctacttga gccgactcgt atctatgtca aggctgtctt gccgctcatc aaagaagagt
    10801 tggtcaacgg cattgcccac atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt
    10861 ttgcagatga cctagctgct gaaattgatg aaagcaaagt tccagtgctt ccaattttca
    10921 aagcccttga aaaatatggt cagattaaac acgaagaaat gtttgaaatc ttcaatatgg
    10981 gtgtgggact tatgttggcg gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg
    11041 atgaagcagt ctatgaaatt ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca
    11101 aatgaaaaaa atagcggttt ttgcctctgg taatggctca aattttcagg tgattgccga
    11161 agaatttcca gtggagtttg tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc
    11221 aaagcagctc ggcgttctgt cctatgcttt tgaactcaag gagtttgaga gcaagacaga
    11281 ctacgaagca gcccttgtcg aactcttgga agaacaccag attgacttgg tttgcctagc
    11341 aggctacatg aaaatcgttg gaccaacctt attgtcggct tatgaaggtc ggattgtcaa
    11401 cattcatcca gcctacttgc cagaatttcc aggagctcat gggattgagg atgcttggaa
    11461 tgctggcgtg ggtcagtctg gtgtgaccat tcactgggtg gattcgggtg tggatacagg
    11521 ccaggtcatc aaacaggttc gtgtgccacg actagctgat gataccattg acagatttga
    11581 agctcgcatc catgaagcag agtacaggtt gtatccggaa gtagtgaagg ctctatttac
    11641 agattgactt tttgatgatt catatgatat ctttgatttt aaattggagt cagtgtttgt
    11701 tgaagacggc ttcaaacgga ggtatttgta atgttagaat ctaaaaaaac aactcgatat
    11761 gtattttatg tctatctgat gttattaact tggggaatct tatttaagtt tgaaacaaat
    11821 cctgaattta tagcattttt cttagctcca aggtatatca attggattcc attttcagaa
    11881 ccactaatag tcgatggaaa aattgttttt gctgaaatgt tatttaatct gatttccttt
    11941 attccattag gtgtttgttt ccctttgata aaaactaatt tatctagttt aagaatagtc
    12001 gggacaggtt tcttgattag tttattgttt gagtgcttac agtatatttt agcaataggt
    12061 ataacagata taacggattt gactttaaat acgctaggtg tctgtgtagg cttactgatt
    12121 tatcaaattt ttataagagt gttcaaatca cagactagaa aatggatcaa tatcttaggt
    12181 atgcttagcc ttggttttgc ttatcttgtt ttactgttac tgcatttaat tggtgtttaa
    12241 ctaatgatta aaaaggagaa tataatgact aaacgcgtct taatcagcgt ctcagacaaa
    12301 gcgggcattg ttgaatttgc ccaagaactc aaaaaacttg gttgggagat tatctcaaca
    12361 ggtggaacta aggttgccct tgataatgct ggggtggata ccattgctat cgatgatgtg
    12421 actggtttcc cagaaatgat ggacggtcgt gtgaagaccc tccacccaaa tatccacgga
    12481 gggcttctcg ctcgtcgtga cttggatagc cacttggaag cggctaagga caacaagatt
    12541 gagctcattg accttgtggt ggtcaacctt tacccattta aggaaactat ccttaaacca
    12601 gatgtgactt atgctgatgc agttgaaaat atcgatattg gtgggccgtc tatgcttcgt
    12661 tcagcagcga aaaatcatgc cagtgttaca gttgtggtag atcctgctga ctacgctgtg
    12721 gttttggacg aattggcagc aaacggcgaa acctcttatg aaactcgcca acgtttagca
    12781 gccaaagtat ttcgtcacac agcggcttat gacgccttga ttgcagaata cttcacagct
    12841 caagtgggtg aaagcaagcc tgaaaaactc actttgactt atgacctcaa gcaaccaatg
    12901 cgttacggtg agaatcctca acaagacgcg gacttttacc agaaagcttt gcctacagat
    12961 tactctattg cttcagccaa acagctcaac gggaaagaat tgtcatttaa taatatccgt
    13021 gatgcagatg ctgctatccg tatcatccgt gacttcaaag atagtccaac cgttgtggct
    13081 ctcaaacaca tgaatccatg tggaattggt caagctgatg acatcgagac tgcttgggac
    13141 tacgcttatg agtctgaccc agtatctatc tttggtggga ttgtcgtcct caaccgtgag
    13201 gtggatgctg cgacagctga gaagatgcac ggcgttttcc tcgaaatcat cattgcacca
    13261 agctatacgg atgaagcgct agctattttg atcaataaaa agaaaaactt gcgtatcctt
    13321 gccttgccat ttaatgctca agaggctagc gaagtggaag cagaatacac aggtgtagtc
    13381 ggtggacttc tcgtgcaaaa tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg
    13441 gtgaccaaac gtcagccaac tgagacagaa gcgactgctc ttgagttcgc ttggaaggct
    13501 atcaagtacg tcaaatcaaa tggtattatc gtgaccaacg accacatgac acttggtgtt
    13561 ggtccaggtc aaaccaaccg tgtggcttct gttcgccttg ccattgacca agccaaagat
    13621 cgtctgaacg gggcggtcct tgcttcagat gccttcttcc catttgcgga taacgtggaa
    13681 gaaatcgcca aagcaggaat taaggccatc atccagcccg gtggctctgt ccgtgaccaa
    13741 gaattcatcg aagcagcgga taaatacggc ttgactatgg tctttacagg tgtgagacat
    13801 tttagacatt aagaagataa aagggaagaa aacagtttct ttcctttttt ggcttaaaat
    13861 gctaactgaa acaagattaa aacgaacttt tttgatataa tgttggtaaa taattcgcaa
    13921 aagaggttga ggaatgaaac tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc
    13981 taaaaagtta cttgaatcaa aagacgtgga aaaagtcttt gtagctcctg ggaatgacgg
    14041 tatgactctg gatggtctgg aattggtaaa tatatctatt tccgaacatt ataaattgat
    14101 tgacttcgca aaggccaacg atattgcttg gacctttatc ggtccagatg acgcccttgc
    14161 ggctggtatc gtggatgatt tccatgcagc tggtctcaaa gcctttggtc cgacaagatt
    14221 ggcagcggag ctggagtggt ccaaggattt cgccaaggaa atcatggtca aatatggtgt
    14281 ttcgacagca gcctatggca cattttcaga tttcgaggaa gcaaaggcct atatcgaaaa
    14341 gcatggtgcg cctatcgtag tcaaggcgga tggcttggcg cttgggaagg gtgtcgtcgt
    14401 tgctgagacg gttgagcaag cggtcgaagc cgctcatgag atgcttttgg acaataaatt
    14461 tggtgactca ggtgcgcgcg tggttattga ggaattcctt gaaggagagg aattttcact
    14521 ctttgccttt gtcaatggtg ataagttcta catcatgcca acggctcagg accacaaacg
    14581 tgcctacgat ggcgacaaag ggcctaacac gggtggtatg ggtgcctatg cgccagttcc
    14641 acacttacca cagagtgtag ttgatacagc ggttgacacc attgtcaagc cagttctaga
    14701 agggatgatt aaagaaggtc gcccttatct gggagttctt tacgcagggc ttatcctgac
    14761 agctgatgga ccgaaagtca ttgagttcaa cgctcggttc ggagatccag aaactcagat
    14821 tatcttgcct cgcttgacct ctgactttgc tcaaaatatc acagatatcc tggatagcaa
    14881 ggagccaaat atcatgtgga cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa
    14941 gggctacccg ctagactatg aaaggggcgt tgagttgcca gccaagacag aaggcgatgt
    15001 catcacctac tatgcagggg ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg
    15061 cggacgagtt tatatgctcg ttaccacagc agataccgtc aaagaagccc aagccagcat
    15121 ctaccaagaa ctatcccaac aaaaaataga aggactcttc taccgaacag atatcggaag
    15181 caaggcaatt aagtaaagat ataagaataa cgcgccgtag tcgccaaaca cgataatggt
    15241 cgtcgtggtg aaaagaccag aacagtgaat gttctggtca gggggaaact tggagaccgt
    15301 aggctcaaag tttaggaatg aaaccgaagg tttgcttccg cctccatcac ctaagaccat
    15361 tatcaaaaag aaaaataaaa attcacaaaa tacgttaatg atcgtatggt ttgcgagcgt
    15421 tagcgagcta atatagaaca atcaccgccg ttgtgaaaga acgattggat gataatccaa
    15481 tcgttcaggg aaattggaag accttgggtt tccaatttag gcatgagaca cctttggtgg
    15541 ctgctgccgt ccctcacaag ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat
    15601 gaaaccagta atttccatca tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac
    15661 agcagaagtc ctagaccgct tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg
    15721 tacaccagac ctcatgttca aacatgcaga agaagcccgt agtcgtggca tcaagatcat
    15781 catcgcaggt gctggtggcg cagcgcattt gccaggcatg gtagctgcca aaacaaccct
    15841 tccagtcatt ggtgtgccag tcaagtctcg tgctcttagt ggagtggatt cactctattc
    15901 tatcgttcag atgccgggtg gggtgcctgt tgcgaccatg gctatcggtg aagctggagc
    15961 gactaacgca gctctctttg ccctccgtct cctctctgta gaagataagt ccattgcgga
    16021 tgcacttgcc aactttgctg aagaacaagg aaaaatcgca gaggagtcgt caaatgagct
    16081 catctaaaac aatcggaatt atcggtggcg gtcaactggg tcagatgatg gccatttctg
    16141 ctatctacat gggccacaag gttatcgcgc tggatcctgc ggcggattgc ccggcctctc
    16201 gcgtggcgga aatcattgtg gcaccttata acgatgtgga cgccctccgt cagttggcag
    16261 accgttgcga tgtcctcact tatgagtttg aaaatgtcga cgctgacggt ttggatgccg
    16321 ttatcaagga tggacaactc cctcaaggaa cagatctgct ccgcatttcg caaaatcgta
    16381 tttttgaaaa ggactttttg tcaaacaagg ctcaagtcac tgtggcaccc tacaaggtcg
    16441 tgacttctag cctagacttg gcagatatcg acttgtcgaa aaactatgtc ctcaagactg
    16501 cgactggtgg ctacgatggt catggacaaa aggttattcg ttcagaagca gacttggaag
    16561 cagcctatgc gctagcagac tcagcagact gcgtcttgga agaatttgtc aactttgacc
    16621 ttgagatttc tgtcatcgtg tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg
    16681 aaaatatcca ccgcaacaat atcctgtcta agaccatcgt accagcccgc atttctgaaa
    16741 gtctagtaga caaggctaaa gctatggcag tgcgaatcgc agaacaactc aacttgtctg
    16801 gaactctctg tgtggaaatg tttgcgactg atgatgacgt tattgtcaat gaaatcgccc
    16861 cacgaccaca taactctggg cactattcta ttgaagcctg tgatttctct cagtttgaca
    16921 cccatattct cggtgttctc ggagcaccat taccagtcat caaactccat gcgcctgccg
    16981 tcatgcttaa cgttctcggc cagcatgttg aggccgctga aaaatatgtc acagaaaatt
    17041 caagcgccca cctccacatg tatggtaaaa tagaagcgaa gcacaaccgc aagatgggac
    17101 atgtgacttt gattagtaat gaaccagata gtgtgggaga gttttagggg agaggggtct
    17161 aaagaaaaaa tgtttgaaaa aattaaagga attaatataa aatcaggtat ttttgaagat
    17221 gaaacaaaat tagaactctt tgaaggaaat tttgagggaa caaatccagt acaaaacgat
    17281 agggcaagtt tattgtttgg aaggaatggt tcagggaaaa gtaccattgc tcgaggaata
    17341 aatcaattaa aaaatggtga gattggaaca gatagagtat catttattga taaaaataat
    17401 aataatattg tacttagtga tactgaacga aaatcgatat ttgttttcaa tgaacactat
    17461 gttgatcaga aagtcaaaat tgctcaagaa gggttagata caattgtaat tttaggtgaa
    17521 caggttgata tcgatgagga actggataga ttaagaacgc agttatcaga atcacaaatt
    17581 gaatctcaag attattatgc tgaatatgaa gaatatttag atgaaaaaaa tgaaaaatct
    17641 ccagattttt ggaaaaagga aatgacagat tctttgaaag gagtaggcaa ctgggccgaa
    17701 agagataggg agataaaagg aaatcgagct gcatctcccg ttcataataa tacctttcaa
    17761 aattttgttg atttacaacc aatcttagat aaaaatgagt tagaggttga attcaacaat
    17821 aaaaaagcta gatatttttc aataagagat tccgcagtta ctataaataa tgaactatct
    17881 ttacctgata ttaattttga tagcaatgaa ttatcaactt tattgtctga aaaaatcgaa
    17941 gaacctgagt tgaattctag ggataagtat ttactgacat tattatctga ttccacaaaa
    18001 ggtgaacgtc atttgagaga agttaaagat ttctttgaag atgaacatca aaaaaaatgt
    18061 cctttctgta ctcaatcagt atcagaagat gttaaagttg aactcacgaa tggcataact
    18121 aaactactat caagagcagt cgaagaacac caatccgcat tgagaggtaa aaaaattgat
    18181 gagattaatc aagatttttc tggttatgaa cagatagatc caatattaat tcagtcttat
    18241 caaaatagta ttaatgcttt gaatgctaaa tttaacgaaa ttaactcaat aatcgataag
    18301 aagattgata atccatataa tatagttgag ttacctaata taagcttttc tcaagaatta
    18361 agtcaagcag agcatgatat tgaaaaaatt aaccaagcaa ttatcaaaca taattcagaa
    18421 atctcgggta ttcaaaagtt aaaagtagat ctccttcaaa taaataatga attagctttt
    18481 tatgaaattc aagatgcata caaaaagttt caagagaaaa caaataaaaa agcaatttgt
    18541 gaaaataatt ataataacag tagtaaacga gtaaaggatt atgagaagca gatttctgat
    18601 ttagaagaca agaaacttaa tatagatatt gcagttgatg aaattaataa aagtttaaat
    18661 tacatatttt tttcaaaaaa tcggttagct atacagaatc aaaacggaaa atactatctg
    18721 ttatcaagag gtaagtctgt ggtcctcagt agggtttccg taggggaacg aaatgcgctg
    18781 gcattatgtt atttctttac tgagataatt caacagagag agttagcaga tgcttatagt
    18841 catgaatatt ttattgttat tgacgatcca atttctagtt ttgatatgga aaataaagtt
    18901 ggaataacct cgtatttaaa atattgttta actagatttt ttaaggggaa tagtaatact
    18961 agagttttac taatgaccca tgataaacaa acaatatatg actttgatat ctttttgaaa
    19021 gagataatgg aatcctgtaa agaagaggaa ggtggacaaa aatctaaata caagaagtta
    19081 gaattggttt ctggtaaatt acaagaattc aaaactagta ctcacgatta tacagaacta
    19141 cttgaaatag tcttcggtta tgcattggga aatagtacac ctacttcgga aagttttgtt
    19201 gggaatgcaa tgagaaaaat tttagaagcc tatggtagtt ttaattataa gaagggaatt
    19261 gccgagctaa caacagatcc tttaattgtt gaaaaaattg ataaagaata cagaacttat
    19321 tttgagaatc ttatgtatag actggtttta aacggtgaaa gtcattttaa ggatccggtt
    19381 aaaactttga gtattgattt ctttgatacc atttctgatg aagaacgaaa aaaaacagct
    19441 agagatttac tagttttgct ttatctttta gatgatttac atgttttaaa acatttagag
    19501 ggtgtatcaa atgcggaaaa tagacttgaa caatggaagt gtgagatttt agaataagag
    19561 aatcatacca tttgacttat tgcaatggtt gaagtttgca tgatagctga ttagagtaat
    19621 caaaggatta ttacttccta attttatttt ctacaaaccc taatttagca tctaataatg
    19681 caaagtttcc tgagcctcat agataaaaaa gatttttaag gagaaaaaca tgatcaaccg
    19741 ttactctcgc cctgagatgg cgaatatttg gagtgaagaa aataaatacc gtgcttggct
    19801 tgaggtggaa atcctctctg acgaggcatg ggctgagttg ggggaaatcc ctaaggaaga
    19861 tgtggctttg attcgcaaga aggcggactt tgacatcgac cgtattttgg aaattgagca
    19921 ggagacgcgc cacgatgtgg tggctttcac gcgtgcggtt tctgagactc ttggtgaaga
    19981 gcgcaagtgg gttcactatg ggttaacttc taccgacgtg gtggatactg cctatggtta
    20041 cctctacaag caggccaacg acatcatccg tcgtgacctt gaaaacttca ctaatatcat
    20101 cgctgacaag gccaaggagc acaagttcac catcatgatg gggcgtactc atggtgtgca
    20161 cgctgagccg acaacttttg gtcttaaatt ggcaacttgg tatagcgaaa tgaaacgcaa
    20221 tatcgagcgc ttcgagcatg cggctgctgg tgtagaagct ggtaagattt ctggtgcggt
    20281 tgggaacttt gccaatatcc caccatttgt agaggagtat gtctgcgata aacttggcat
    20341 ccgtgcccaa gaaatctcta cacaagtcct tcctcgtgac cttcacgctg agtactttgc
    20401 ggttcttgcc agcattgcga cttcaatcga acgtatggcg actgagattc gtggtctaca
    20461 aaaatctgag caacgcgaag tagaagagtt ctttgctaaa gggcaaaaag ggtcttcagc
    20521 aatgcctcac aaacgcaacc caatcggttc tgaaaatatg actggtctgg cgcgtgtcat
    20581 tcgtggtcac atgattacgg cttatgaaaa cgtcgctctc tggcacgaac gcgatatttc
    20641 tcactcatca gctgagcgta tcatcacacc agatacgacc attttgattg actacatgct
    20701 caaccgtttt ggaaatatcg tcaagaactt gacagtcttc ccagaaaata tgatccgaaa
    20761 catgaactcg acttttggtc ttatctttag ccaacgggct atgttgacat tgattgaaaa
    20821 aggcatgacc cgtgagcaag cctatgactt ggtgcaacca aaaacagcct actcttggga
    20881 caaccaagta gactttaaac cacttcttga ggcagattca gaagtaacat cacgtctcac
    20941 acaagaagaa atcaatgaaa tcttcaaccc aatttattac accaaacgag tggatgatat
    21001 ctttgaacgt cttggactag gtgattaatt aaaaaataaa cagcgagctt caatctcgct
    21061 gtttattttt tatcgaaaag acttagtctt cttttctttt agtgagtcca taggctgcta
    21121 gtgtggacat gagtcctgcg actactagtc ctgcagaatc gtgagttcct gtttcaggaa
    21181 gttttttctc tgttaccaca ggagctggat cttgaggaag agctttgctt tcctcagcag
    21241 gagcagttga tggagctggt tggcttggga tttctagtct tggtttttct tcagcaatag
    21301 cggcttgtcc gttttcatcg cctacatgtg ttaccatagt tccgacttcg actatttgag
    21361 taacggcttc ctgtgctacg acactattta caagtgtttt cacttcctta ccatcggcag
    21421 aagtgctcac agagtagaag ttgctacgat gtccattgac gcccttagta atgacttgtg
    21481 tttttccttt gagtaagagt ggattttcac gagtcactgt ggtaaatgga atttcttctt
    21541 tttggatatc cagtctaggt tttacctcag tagttggtgc aagaccactt tcatcaccct
    21601 tgtgagttac aggagcgcca acttcaacca cttggtttat aacttctttg gttacctggc
    21661 tatcaaggac tgtttctgtt gtttttccat tttcagtgag tacagagatg taatgagttc
    21721 gttcaccttt gactcctgct gtgataatat tttcctgacc agctgggagg ttaggatttt
    21781 ctttcttgat aacttcaaat ggaatttctt cagttcttgt gatgagttct ggtctggttt
    21841 caacattggc agccacttca ttttcatcca ggcttcctga atgagttgca gctggtttga
    21901 ggccttgaag agcggctttt aggttggcta caagtgtgtc aagctcagct tgtttattac
    21961 ggttgaggtt gtaatttaga gctgttttag ctgcgtcaag ggcctcaaga ctttctttac
    22021 tatatccttc taagtttgta ggaattttag ctaattcttc gcggagagca ttataattag
    22081 cacggaagta gtctttgttg tggtctgcaa aggcagtcat gagttcaaag atttcctctt
    22141 ccttgtactc agcgcttggt ctatctgccc agattgaaag catacttccg actgttggaa
    22201 gatctacttc aggatatttg gtagaagcta gttgattgaa tggaacttta ccagaatttt
    22261 caactgcttt tgaaagaggg taagcttcat cttgtttgtg attgcctata acataatacc
    22321 agtcaccgtt ggtattcaag aatttatagc ctttgcttgc taggtattga ggtgatgcga
    22381 ggttatatcc ccaccagcct ttagaccagt aagaaatcaa gacatctttg tcaaactgaa
    22441 catcgtcctt gtcttcatag tagaagccat cgttgaaggc cattggttga agccctcttt
    22501 ctttggccat agctgcgagg gtgttggcat attcggcaaa tttgccatag agttgatacc
    22561 acttgaggta gtaccagcct tgggcactag tcgcatcgtt ggcgtattcg tcagtaccaa
    22621 agttgaaaat ctttgtttta cctgcaaaga agtccatgta tttaccgatg agggctttta
    22681 caaagttcat cgcttcttcg tttttcaagt ccatagttgt ttttgaaact ttatcaaagt
    22741 gggcttgagg atttttaata cctaattttt ccatggcaac cagcatagca tccatgtgac
    22801 ctggactgtt aatagctggg atgagaccaa tgtccttaga tttagcgtat tcaattagct
    22861 ctgtcacttc tgcctgtgtt agtgcagtac cgtttggatc gtcgtagtaa gctttagttc
    22921 cttcgataat agctttttta acgtcatcac tagcataggt ttttccgttg gcagtaatgg
    22981 tcatatcatt gagtagaaag cgaagtccgt catttcctag aaggagatgg acatcagaat
    23041 atccgagctc actggccttg tctacgatgc gtttgagctg gtccagagta aagtatttgc
    23101 gtccagcatc gattgagatt accttgtttt tggcaagttt ttcaacctca cgtttagctt
    23161 cttcttcttt ttgagcttca ggcgtgaggg tcaagttgtt gacagtttct tgaagtttag
    23221 caatggcttg atcaatcgta tcttgttggg cacggctaag gttgctatcg agagagcgaa
    23281 tagctttttc agcttctttt acggccgcga cgctttctgc agtataacgg ttcaggtctt
    23341 ttggtacctc gttaagtgct tgctctgcag attcataatc agctgcgaag tattcagcgt
    23401 tggcatttgc aaaatgacgc atgagtttga agaggtgtga tggtgaataa cgtgcagatg
    23461 gagtgtcagc ccaagcagct accataccac cgatgattgg gatatcagct ccttctgttt
    23521 ttggtacaga agtgattggt gtgtttttaa taccattgag cccctgatcg agattgtacc
    23581 agccttggcc atcagcgttt cgtccaagaa cgtagtacca agcatcattg gtattaagga
    23641 tttggtgacc tttttcagct agtagtttag aagaagcgac atcgtagcct ccccaaccac
    23701 cagtccacat agaaacgatg atgtctttgt caaaactacc aaagcttgtg tcgctattgt
    23761 agtagatacc gtcgttaaag gccattggtt tgagaccgtg cgattttaca atacgagcga
    23821 ggtcattggc gtaggcaata aatttttcat agccttttac agggtagcct tcgtttggat
    23881 agtatttatc agcttgaagc acactccaac ctttagcatc tgtcgcatca ttggcatatt
    23941 catcaagtcc gatgttgaag atttcagtct ttttcgcgaa ataagcagca tacttgtcga
    24001 taagggcttt tgtaaaagcg acagcttgtt cgttgtcaag atcgacagta cgggctgatt
    24061 tcttcccaaa atagctaaag ttagggtttt ggattcccaa ttctttcatg gcattgagaa
    24121 tcgcatccat gtgtccagga ctatttactg tcggaatgag accgatacct ttatctttgg
    24181 catagttaat cagatctgtc atttgacttt ctgttaagtg attgccgttt ggatcgttgt
    24241 aataatcatt tgtacctttt tcaatggcgc gtttgacatc gtcactggca taggtcttgc
    24301 cgttagctgt gatgctcata tcgtccaaca tgaaacggag tccatcattt ccgactaata
    24361 ggtgtaaatc agtgtagcca taatgtttcg ctttatcgat gatttccttg agctgttctg
    24421 gtgagaaata tttacgtcca gcatcaatag aaacaatttt ctttttcgct agtttttcat
    24481 ttacagttgc agcacgttcc tttcctgtct ctgtttccgg tttgtcagcc tctgctttcg
    24541 cttcatcttt tttagctggt ttatccttgt cagtcttgtc tgtatttgac tctttagaat
    24601 caacctcttt cgcttcttcc tttttagggc tagcttcttc ttccttttta ttagcagttt
    24661 ctttttcagc agaagttgga gttaccacct ctgctttatc actaggagtt gaactaactt
    24721 cctcttgtgg tttttcttct gtttttggaa gactagctac cttatcagta gctggagttt
    24781 ctgtttctac agtttttgga gcttctggtt gaagctctgc tttaggtgtt tcctcagtcc
    24841 gattttcgga tgattgaggg gaatcagaaa ccgtatggat ggtcggttgg ttttctgtag
    24901 tagtaggagt aactccatcg gctgcaacag tctgtgcttg gaaggcaaat ccaattagaa
    24961 cagaagctgc tcctacagcg tatttacgaa tagaaaaacg ctgttgtttt tcatgtttca
    25021 ttgcaaaacc tcctgattgc attgttatat tgatagcgat tatataaatc aacgccttta
    25081 ttttatttct tatattaatg agagtcaaga ggagatgaca aaaaactata ataagtataa
    25141 aaaaatataa aatttaaact taagatttca gattggtcga aaaaatacat atatatatct
    25201 agtataattt ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa
    25261 taggtaagcg ctaccttttt ggtgtggtat aataagcata gaaaaagccc gagcgattag
    25321 gctcaggctt tcttcttagt gatcacggtc acatgagatg aatttaatct tgtagtaatc
    25381 agatcgtttg taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat
    25441 tttagtttgt aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc
    25501 tggagttgga aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg
    25561 gtagtctaac ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc
    25621 gttgatatat tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc
    25681 gcggatacgt tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaagta
    25741 aacaagcttg tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa
    25801 gagttcaata tctgaaaact ctacaagctt gtgtttgcgt gcacgtgaaa cgaaggttcc
    25861 ttttccttgt tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac
    25921 tgtgatagag ctgacatcgt acattgaaat gagttctgct tcagtgtaaa atttatctcc
    25981 actgctaaac tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt
    26041 gggaattgcc atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc
    26101 gaaaaaaata gtagtctttt gaataaaaaa ttagaataat agtttgaaaa attaagcgat
    26161 atagggattg ctcatatttt atcaatgcat tctatacaaa attattttag gtcctatatt
    26221 ccgaatttaa agagcgtttg aaatgggtct aaaaatgttt gaaactaatt gaatgtgtgt
    26281 ataatagtaa aaatgcagta aatatcccga caatttctat tgacaattca aacagattgg
    26341 tttataatta atataacaac aaatgaaagc gcaaactttc gcggtcggaa ggtagtttta
    26401 tgacacgatt tgagatacga gatgatttct atctcgatgg aaaatcattt aagattttat
    26461 ctggtgccat tcattatttt aggattcctc cagaggattg gtatcattcg ctctataact
    26521 tgaaggctct tggttttaat acggtagaga cttatgttgc ttggaattta cacgagcctc
    26581 gtgaaggtga gtttcatttt gaaggtgatc tggatttaga gaaatttctc caaatagcgc
    26641 aggatttggg tctctacgca attgtgcgtc cgtctccatt tatctgtgcg gaatgggaat
    26701 tcggtggctt accagcttgg ctcttgacca agaacatgcg aattcgctca tccgacccag
    26761 catatatcga ggcagttggt cgctactatg atcagttatt gccaagactg gtgcctcgtt
    26821 tgttgaacaa tggtggcaat attctcatga tgcaggttga aaatgagtat ggttcttacg
    26881 gagaagataa ggcttacctg agagcgattc gacagctaat ggaagagtgt ggcgtaacct
    26941 gtcccctctt tacatcagat ggtccatggc gagctactct gaaagctgga accttaattg
    27001 aagaggacct ctttgtaaca ggaaactttg gttctaaggc accttacaac ttttcgcaga
    27061 tgcaggaatt ctttgatgaa catggtaaga aatggccact catgtgtatg gagttctggg
    27121 atggttggtt caatcgctgg aaagaaccga ttatcacacg ggatcctaag gaattggcag
    27181 atgcagttcg agaggttttg gaacaaggct ctatcaatct ttacatgttc cacggtggta
    27241 caaactttgg tttcatgaat ggttgctcag ctcgaggaac tttggacctg ccacaagtta
    27301 catcttatga ttacgatgcc cttctggatg aagaaggaaa tccaactgct aaatatcttg
    27361 cagtcaagaa gatgatggca acacattttt cagagtatcc gcagttggaa ccactctaca
    27421 aagagagtat ggagttggat gctattccac tagttgaaaa agtttctttg tttgaaacct
    27481 tagatagctt gtcaagtcct gtagaaagtc tctatcctca aaagatggag gagctgggac
    27541 aaagttatgg ctacctactt tatcgaacag aaacaaactg ggatgcagaa gaagaaagac
    27601 ttcgtatcat tgatggtcga gatagggccc agctgtatgt cgatggtcag tgggttaaaa
    27661 ctcaatatca gacagagatt ggggaagata ttttttatca aggtaaaaag aaagggctat
    27721 ctaggttaga tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat aagttcttag
    27781 cggatacgca acgtaaggga attcggacag gggtctgtaa ggatctgcat ttcttactaa
    27841 actggaaaca ctatccactc ccactagaca atcctgagaa aattgatttt tcaaaaggat
    27901 ggactcaagg acaaccagcc ttttacgctt atgactttac agtcgaagag ccaaaagata
    27961 cttacctaga cttgtctgag tttggtaagg gagttgcctt tgtcaatggg cagaatctag
    28021 gacgtttttg gaacgttggc ccaactctct cactttatat ccctcatagc tatctcaagg
    28081 aaggtgccaa ccgtatcatt atctttgaaa cagaaggtca atataaagaa gagattcatt
    28141 taactcgtaa acctacacta aaacatataa agggggaaaa cttatgacaa ttgtaggatg
    28201 ccgtattgat ggacgtttga tccacggaca agtagccaat ctttgggctg gaaaactaaa
    28261 tgtttcacgc attatggttg tagacgacga agttgtcaac aacgatattg aaaagagtgg
    28321 tttgaaactt gcgacaccac caggtgtgaa attgagtatt ttgccagttg agaaagctgc
    28381 agccaatatt cttgctggta aatacgatag ccaacgtctc tttatcgtgg ctcgtaaacc
    28441 agaccgcttc cttggtttgg tagaagcagg tgtaccactt gaaaccctta atgttgggaa
    28501 tatgtctcaa acaccagaaa ctcgttctat tacacgttct atcaacgtag tagacaagga
    28561 tgtggaagac ttccacaaac tggcagaaaa aggtgttaaa cttactgctc agatggttcc
    28621 aaatgatcca atttcagact ttttgagctt attaaaatag gaaaaaaatt tttaggaggt
    28681 cattgttatg atacaatggt ggcaaatttt acttctcact ttgtactcag cttatcaaat
    28741 ctgtgatgag ttgacgatcg tttcatctgc aggttcccct gtatttgctg gtttcattac
    28801 tggtttaatc atgggagatg tgactactgg tttacttatc ggtggtaact tgcaactgtt
    28861 cgttcttggg gttggtacct tcggtggtgc ttctcgtatc gacgcaactt ctggtgcggt
    28921 tcttgcgaca gccttctctg tttcacaagg aattgatgca ccgcttgcca ttactacaat
    28981 cgctgtacca gtagcagctc tcttgactta cttcgacgtt cttggtcgta tgactactac
    29041 cttcttcgct caccgtgtgg atgctgcaat cgaacgcttt gactataaag gtattgaacg
    29101 caactacttg cttggtgcga ttccgtgggc tctatctcgt gcccttccag tcttctttgc
    29161 ccttgctttt ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag cctacaaatg
    29221 ggttgcagat ggcttgacac ttgcaggacg tatgcttcca ggtcttggat ttgcaatctt
    29281 gcttcgttac cttccagtta aacgtaacct tcactacctt gctatgggat ttggtttgac
    29341 agctatgttg actgttcttt actcatatgt aacaggtctt ggtggcgctg ttgctggtat
    29401 cgtaggtact cttcctgctg aagttgctga aaaaattggt ttcgtgaaca acttcaaagg
    29461 tttgtctatg attggtattt ctatcgtagg tattttcctt gcagtgcttc acttcaaaaa
    29521 tagccaaaaa gtagctgtag cagcaccttc tacaccatca gaaagtgggg aaatcgaaga
    29581 tgacgaattc taattacaaa cttacaaaag aagattttaa tcaaatcaac aaacgtagct
    29641 tgtttacttt ccaattaggt tggaactacg aacgtatgca agcttctggt tacctttaca
    29701 tgatcttgcc tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg aaagaaatga
    29761 tgaaagttca tactcaattc ttcaatactt caccattctt ccataccatt atcgctggtt
    29821 ttgaccttgc catggaagaa aaagatggtg taggttcaaa agacgccgtt aacggtatca
    29881 agacaggttt gatgggacca ttcgctcctc ttggggatac aatctttggt tcacttgtac
    29941 ctgctatcat ggggtcagtc gcagcaacta tggctatcgc tggccaacct tgggggatct
    30001 tcctttggat tgcagttgca gtagcgtatg acatcttccg ttggaaacag ttggaatttg
    30061 cttacaaaga aggggttaac cttatcaaca acatgcaaag taccttgaca gctttgattg
    30121 acgctgcatc tgtacttggt gtcttcatga tgggtgctct tgtagcaaca gtgattaact
    30181 ttgaaatttc ttacaagttg ccaatcggtg aaaagatgat tgatttccaa gacatcttga
    30241 accaaatctt cccacgtttg cttccagcaa tctttactgc ctttatcttc tggttgcttg
    30301 gtaagaaagg tatgaactct actaaagcta tcggtattat tatcgtactt gctttggctc
    30361 tttctgccct tggtcacttt gcacttggaa tgtaattcct tatgactaaa tcattaattt
    30421 tggtgagcca tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg attatgggcc
    30481 cacaagacaa catttacaca gtagctcttc ttccagaaga tggcccagaa gaatttactg
    30541 ctaaatttga agctgttatt gaaggattgg atgatttcct agtctttgcg gatcttctcg
    30601 gtgggacacc ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat attgaccttt
    30661 acgcagggat gaatcttcca atggtgattg aatttatcaa tgcgagcctt acaggcgcag
    30721 atgcggacta caagagccgt gctgcagaaa gcattgtgaa agttaatgac ctgttagcgg
    30781 gcttcgatga tgacgaagat gaataatact cttcgaaaat ctcttcaaac tacgtcaacg
    30841 tcgccttgcc gtaggtatat gttactgact tcgtcagtct tatccggcaa cctcaaaacg
    30901 gtgttttgag ctgacttcgt cagtcttatc cggcaacctc aaagcagtgc tttgagcagc
    30961 ctgcggctag tttcctacag attttagttg gaactcgatt caattcatgt gacaacgtga
    31021 aaatcgttag agcattttat atagaatata catgggaatg tagcttactc ccattcccat
    31081 atttaataga aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg
    31141 ggtgcagaaa tcactacgcg tgaaatctac caacagcctg atgtatggag agaagctttt
    31201 gaattttatc aagcaaaacg tgaagaaatt gcagccttcc tacaagaaat cgctgataaa
    31261 catgactata ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagatacc
    31321 ttgctacctt attttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg
    31381 acaacagata tcgttgccaa tccagcaacc tatttgaaaa aagatgtggc aactgtcctt
    31441 gtgtcttttg ctcgtagtgg gaattcgcct gaaagtttgg cgactgttga tttggccaaa
    31501 tccttggtgg atgagcttta tcaagtgacg attacttgtg cagcagatgg taaattggct
    31561 cttcaagctc acggtgatga tcgtaatctc ttgctcttgc aaccagctgt ctctaatgat
    31621 gctggatttg ccatgacttc tagctttacg tctatgatgt tgacaactct cttggtcttt
    31681 gatcctacag aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt
    31741 aaagttttag acaaggcaga agatgtcaaa gagctcgttg atttagactt taaccgtgtc
    31801 atctatctag gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg
    31861 gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt
    31921 ccaaaatctc ttatcaacga caatacagtt gttttggtct ttggtacaac gacagactac
    31981 actcgtaagt acgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt
    32041 gttgtgcttt tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggcccttggt
    32101 tgtggcggtg tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc
    32161 tttgctttat tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt
    32221 acagtaaacc gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt
    32281 tatgaattct tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc
    32341 tttctatggt ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac
    32401 agagcgtgta tttggaaatg ttgagggtga ggatgtcttg gcctatcgat ttgagacaga
    32461 cggtggctac caacttgagg ttatgactta tggtgcgact atcttgcgct atgtcgcacc
    32521 tgacaaggct ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt
    32581 aggcaatagt cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc
    32641 gacctttgag ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa
    32701 tcacagtggt tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca
    32761 tggcttgact ctctacacag agcgtacaga tgggacagga gggttccctg gaaatctcaa
    32821 gatttggatc agttatcact tggaagaaac tggtgcctat gaaatcagct acaaggtaac
    32881 gaccgatcag gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga
    32941 tttcacgcag acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat
    33001 cgctcctgac ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacgt
    33061 ctacaatggt accttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc
    33121 atcaggtttg gatcatccat ttgcccttcc tgcaggccat gacaatgctg gattccttta
    33181 tgaccaaaat tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta
    33241 cacagcaaac tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa
    33301 tgggattgct cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca
    33361 agtcattctt aaagctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa
    33421 ataaaaaaag ctggtcaaaa agtctttaaa attaaaataa cgcatattgt caggtgatga
    33481 aaagtttgat aatatgcgtt ttattatgaa atgatttact ttatattatc ctgaaatggt
    33541 gttatttttg agttatctgg cttgactttc ttgatggaaa ttatataata gttgtaacaa
    33601 taataattac aactagaaaa gaggaccatt tatgacctat gaatacaaga gtcacattta
    33661 tttggcagag acagctttaa atgtaaagga tttggccagt caaacagcct tttatcagca
    33721 agttattggt ttagaaatct tatctcaaac tgagacagag tccattctag gccttggtgg
    33781 aaaagtcttg gtacagctga ttcaagcaca agagagcgga gaagtgaggg aatattatgg
    33841 tctttaccat ttggccattc tcttgcccac acgcaaggct ttagctgatg tcttgaaaca
    33901 cctgacagat ttacaaattc ctcttgtcgg cggtgcagac catggttaca gtgaagctat
    33961 ttacttggaa gatttggagg gaaatggcat tgaactctat cgagataagc cagtttccac
    34021 atgggatatt cgagaagatg gacgcattat cggagtgacg gaggctcttg cggcgcagga
    34081 catttatgag ttgggggaaa gagtagagcc ctttatcctg gcagaaggta caagaatggg
    34141 gcatattcat ctttccgtca aggatagtcg aaagtccagc cagttttatc aaaaggtgtt
    34201 agggctagag aataaattca gtgtgcctgg cgctagttgg attgcggctg gagattacca
    34261 tcatcattta gcagttaacg aatggggagg aaaaggtctg gcttcgcgta agcaaggctt
    34321 accaggttta gcctactatg tcatcgaagt cgcacataaa aaagaactgt taacgattgc
    34381 ccaacgagca caagaagttg acgcaccaat caaatggatg acatcgatcc aattggaaat
    34441 cacagactca gatggcatcg tgacccgtat tcgtttagca aggtaaaatt aggaattttt
    34501 aataagttat aagtattgct agtttgcttt ttgaacggtc tttggtaaca gaactgcgtg
    34561 agaattttga caaagaagct gattagagat ataatgaaga aaaatcgagc aaggaaaaag
    34621 aactgacaaa ttcaaagtat attacttgtt tgaaacgttc agagggccag ttgtgtggga
    34681 ttcaaaagat gattgaagga gatcgtgact gtgctgatat tgtgacgcaa ctgacagcag
    34741 tgaaatctag tgtagagcgc gtgattgaga tgataattac cgaaaatctt actgaatgta
    34801 tcaatcagcc cctagatgat tctgaagctc aaaaggaacg cctagaaaag gctatccgat
    34861 acttgattaa acggaaataa atgaatgttt tgagacgata tgtgcaaagg tctattaact
    34921 attagaatag tttaatgtga gtcaaaagaa gttgtaaaaa atctctaaaa ttaaaaaaac
    34981 gcatagtatc aggtgttgaa tgtactgacc cccaaaagtt agattttttc tgtctaactt
    35041 ttggggtgca gttcataaga accttggtaa tatgcgtttt attatgtaga gacttatact
    35101 cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc gtagatatgg ttactgactt
    35161 cgtcagttct atccacaacc tcaaaacagt gttttgagct gacttcgtca gttccatcca
    35221 caaccttaaa acagtgtttt gagcagcctg cggctaactt cctagtttgc actttgattt
    35281 tcattgagta ttacttcatc ttttcctgaa attgagtttt tgcccaactc ctcttgatta
    35341 ttctacttga ccgggccaag cattcatacc accttctacg ttggtaacgg tgaggccttg
    35401 ggagctgaga aactggcagg tagatgcaga gcggactcct ccttgacaaa tgacatagta
    35461 ttcatggtca ggtttgagtt ctttgtagcc ttgctctaag gtacttaatg gaagattttt
    35521 ggcacctggt gcatgtcctg cttggaattc atgtgcttca cggacatcga taagttctag
    35581 attttcattt tggtattttc cataaaagtc agccatgctg atactagttt ccatattcta
    35641 ctccttctgg gttaaccatt ttgtatagtg aataagcgcc gtcaaggttt tgggcggtaa
    35701 atcctgcttg cttgaggata ggctctgcga tatagctgcg caaaccactg tggcagctga
    35761 cgatgtaggc ttgttttttg tccagttcat cagtttcctc cttgacactt tgtcagtttt
    35821 tgatacaata gtacaaaatt agaggaggca ggctatgatt cagaaacatg cgattcctat
    35881 tttagagttt gatgacaatc ctcaggcggt tatcatgccc aatcacgagg ggctggactt
    35941 gcagttgcca aagaagtgtg tttatgcatt tttaggtgag gagattgacc gctatgcaag
    36001 ggaagtaggg gcggattgtg tcggcgaatt cgtttctgct accaagacct atccagtcta
    36061 tgtcatcaac tacaagggtg aggaggtctg tctggctcag gctcctgttg gctccgctcc
    36121 agcagcccag tttatggatt ggttgattgg ctatggtgtg gagcagatta tctctactgg
    36181 gacctgtggt gtcctagctg atatagagga aaatgccttt ctagtccctg ttcgcgcttt
    36241 gcgagatgag ggagccagtt accactatgt ggcaccttgt cgttatatgg aaatgcagcc
    36301 agaggctatt gctgctattg aggaagtttt ggaagacaga gggattcctt atgaagaagt
    36361 catgacctgg acgacagacg gtttttaccg agaaacggct gaaaaggtgg cttatcgtaa
    36421 ggaagaaggc tgtgctgttg tggagatgga gtgttctgct cttgcggcag tagctcaatt
    36481 gcgtggggtt ctctggggtg aattgttgtt cacagcagat tctctagcgg acttggacca
    36541 gtacgacagt cgtgactggg gctcggaagc ttttaataag gcgctagaac tgagtttagc
    36601 aagtgttcac cacctttagt tgtactggca aaggatttgt tttatcataa aatgtctagc
    36661 tcatactttt caaaaatatg tttaaacgag gtcaccttcc tcttgtccta ggcatgttga
    36721 ggttgggaaa aatctttaaa atcagaaaaa cgtatcatat caggtgatga aaattttgat
    36781 actatgcgtt ttatgttggt aagatttaga gtgagatgaa atgatactct tcgaaaatct
    36841 cttcaaacca ggtcagcttc accttgccgt atagatgtta ctgacttcgt cagttctatc
    36901 tgcaacctca aaacagtgtt ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca
    36961 gtgttttgag caacctgtga ctagctttct aagcgatgcc ttggttctca ttgcctataa
    37021 tcaaaaagag aaattttctc ctgaaaagca tatagagtag ctggcgttaa aagctcctgt
    37081 cttgcttttt tgacctatag tcacatctat caagtattgt tcttgcctaa gctatcaata
    37141 aaaaggtggc attttttagg cttggtgtta gtgaattttg ccttatccta tctaagtcat
    37201 ttcgagcttt ttatggtaca atggaaacat gttattcaaa ttatctaagg aaaaaataga
    37261 gctaggctta tctcgtttat cgccagcccg tcgtattttt ttgagttttg ccttggtcat
    37321 tttactaggc tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac
    37381 ttattttgat catcttttca ctgctgtctc tgcagtctgt gtgacaggtc tctcaaccct
    37441 tccagtagct cacacctata atatatgggg ccaaataatc tgtttgctct tgattcagat
    37501 cggtggtcta gggctcatga cctttattgg ggttttctat atccagagca agcaaaagct
    37561 tagtcttcgt agccgtgcaa ctattcagga tagttttagt tatggagaaa ctcgatcttt
    37621 gagaaagttt gtctattcta tttttctcac gacctttttg gttgagagct tgggagctat
    37681 tttgcttagt tttcgcctta ttcctcaact tggctgggga cgtggtcttt ttagttccat
    37741 ttttctagcg atctcagcct tctgtaatgc cggttttgat aatttaggga gcaccagttt
    37801 atttgctttt cagaccgatt tactggtcaa tctggtgatt gcaggcttga ttattacagg
    37861 cggccttggt tttatggtct ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg
    37921 acgtctgcac tttcatacga agcttgtact attgttgact ataggtttgt tgttatttgg
    37981 aacggcaact actctctttc ttgagtggaa caatgctgga acgattggca atctccctgt
    38041 tgccgataag gttttagtta gcttttttca aacagtgacg atgcgaacag ctggcttttc
    38101 tacgatagat tatactcagg ctcatcctgt gactcttttg atttatatct tacagatgtt
    38161 tctaggtggg gcacctggag gaacagctgg gggactcaag attacgacat tttttgtcct
    38221 cttggtcttt gcacgaagtg agcttctagg cttgcctcat gccaatgttg cgagacgaac
    38281 gattgcgccg cgaacggttc aaaaatcctt tagtgtcttt attatctttt tgatgagctt
    38341 cttgatagga ttgattctgc tagggataac agccaaaggc aatcctccct ttatccacct
    38401 catatttgaa accatttcag ctcttagtac agttggtgta acggcaaatc tgactcctga
    38461 ccttgggaaa ttggctctca gtgttatcat gccacttatg tttatgggac gaattggtcc
    38521 cttgaccttg tttgttagct tggcagatta ccatccagaa aagaaagata tgattcacta
    38581 tatgaaagca gatattagta ttggttaaga aaggaaagag catgtcagat cgtacgattg
    38641 gaattttggg cttgggaatt tttgggagca gtgtcctagc tgccctagcc aagcaggata
    38701 tgaatattat cgctattgat gaccacgcag agcgcatcaa tcagtttgag ccagttttgg
    38761 cgcgtggagt gattggtgac atcacagatg aagaattatt gagatcagca gggattgata
    38821 cctgcgatac cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta
    38881 tgcactgtaa gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta
    38941 agaaagtgct agaaaagatt ggagctgact cggttatctc gccagagtat gaaatgggac
    39001 agtctctagc acagaccatt cttttccata atagtgttga tgtctttcag ttggataaaa
    39061 atgtgtctat cgtggagatg aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat
    39121 tagacctccg tggcaaatac aatctgaata ttttgggttt ccgagagcag gaaaattccc
    39181 cattggatgt tgaatttgga ccagatgacc tcttgaaagc agatacctat attttggcag
    39241 tcatcaacaa ccagtatttg gataccctag tagcattgaa ttcgtaaaga gggatgaccc
    39301 ctcttttttg atgcccaaga tggcaaatag agacagaagc cccttgtctt ctagtaaaag
    39361 ttcttcaaag gctggacttt atggtaaaat agaaggaagt gacaagagag agtaatactc
    39421 aatgaaaatc aaagatcaaa ctaggaaact agctgcgggc tgctcaaaac actgttttga
    39481 ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac
    39541 tgacgaagtc agtaacatct atacggcaag gcgacgttga cgcggtttga agagattttc
    39601 gaagagtata agaaaaaatc agttccctaa aggagcagat tatgaagtta ttgtctatcg
    39661 ctatttctag ctataatgca gcagcctatc ttcattactg tgtggagtcg ctagtgattg
    39721 gtggtgagca agttgggatt ttgattatca atgacgggtc tcaggaccag actcaggaaa
    39781 tcgctgagtg tttagctagc aagtatccta atatcgttag agccatctat caggaaaata
    39841 aaggacatgg cggtgtggtc aatcgcggct tggcagaggc ttctgggcgc tattttaaag
    39901 tagttgacag tgatgactgg tggatcctcg tgcctacttg aaaattcttg aaaccttgca
    39961 ggaacttgag agcaagggtc aagagatgga tgtctttgtg accaattttg tctatgaaaa
    40021 ggaaggtcag tctcgtaaga agagtatgag ttacgagtca gtcttgcctg ttcggcagat
    40081 ttttggctgg gaccaggtcg gaaatttctc caaaggccag tatatcatga tgcactcgct
    40141 gatttatcgg acagctttgt tgcgtgctag ccagttctaa ctgcctgaac atacttttta
    40201 tgtcgataat ctctttgtct ttacgcccct tcagcaggtc aagaccatgt actatctgcc
    40261 tgtcgatttc tatcgttatt tgattgggcg tgaggaccag tctgtcaatg agcaagtgat
    40321 gattaagtgc attgaccagc aactcaaggt caatcgactc ttggtagacc agcttgattt
    40381 gtcccaagtg agtcatccca aaatgcgaga atatctgctg aatcatattg aaatcacgac
    40441 ggtgatttcc agtaccctgc tcaaccgatc tgaaacagcg gagcatctgg caaaaaaacg
    40501 ccaattgtgg acctatattc agcagaaaaa tccagaagtc tttcaggcta ttcgtaagac
    40561 catgttgagc cgtttgacca aacattctgt cttgccagat cgcaaactgt ccaatgtcgt
    40621 ctatcaaatc accaaatctg tttatggatt taattaatat aagtgtttta taagagggat
    40681 ttaagaaaaa ttttaacttt ttcttagtcc tttttaattt caggagatta tactagagtc
    40741 atcaaataaa gaaagactct aaggagaatc ctatgaaatt caatccaaat caaagatata
    40801 ctcgttggtc tattcgccgt ctcagtgtcg gtgttgcctc agttgttgtg gctagtggct
    40861 tctttgtcct agttggtcag ccaagttctg tacgtgccga tgtggtcaat ccgaccccag
    40921 gtcaagtctt acctgaagag acatcgggaa cgaaagaggg tgacttatca gaaaaaccag
    40981 gagacaccgt tctcactcaa gcgaaacctg agggcgttac tggaaatacg aattcacttc
    41041 cgacacctac agaaagaact gaagtgagcg aggaaacaaa ctcttctagt ctggatacac
    41101 tttttgaaaa agatgaagaa gctcaaaaaa atccagagct aacagatgtc ttaaaagaaa
    41161 ctgtagatac agctgatgtg gatgggacac aagcaagtct agcagaaact actcctgaac
    41221 aagtaaaagg tggagtgaaa gaaaatacaa aagacagcat cgatgttcct gctgcttatc
    41281 ttgaaaaagc tgaagggaaa ggtcctttca ctgccggtgt aaaccaagta attccttatg
    41341 aactattcgc tggtgatggt atgttaactc gtctattact aaaagcttcg gataatgctc
    41401 cttggtctga caatggtact gctaaaaatc ctgctttacc tcctcttgaa ggattaacaa
    41461 aagggaaata cttctatgaa gtagacttaa atggcaatac tgttggtaaa caaggtcaag
    41521 ctttaattga tcaacttcgc gctaatggta ctcaaactta taaagctact gttaaagttt
    41581 acggaaataa agacggtaaa gctgacttga ctaatctagt tgctactaaa aatgtagaca
    41641 tcaacatcaa tggattagtt gctaaagaaa ctgtcgaaaa agctgttaaa gataacgtta
    41701 a
//
LOCUS       DAVLWA010000008        40262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLWA010000008 DAVLWA010000000
VERSION     DAVLWA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.008"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            5..75
                     /locus_tag="VYN80_000403"
     tRNA            5..75
                     /locus_tag="VYN80_000403"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VYN80_000404"
     tRNA            110..183
                     /locus_tag="VYN80_000404"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VYN80_000405"
     tRNA            266..355
                     /locus_tag="VYN80_000405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VYN80_000406"
     tRNA            366..439
                     /locus_tag="VYN80_000406"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VYN80_000407"
     tRNA            443..515
                     /locus_tag="VYN80_000407"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VYN80_000408"
     tRNA            528..608
                     /locus_tag="VYN80_000408"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VYN80_000409"
     tRNA            613..683
                     /locus_tag="VYN80_000409"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VYN80_000410"
     tRNA            698..770
                     /locus_tag="VYN80_000410"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VYN80_000411"
     tRNA            784..855
                     /locus_tag="VYN80_000411"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VYN80_000412"
     tRNA            865..948
                     /locus_tag="VYN80_000412"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VYN80_000413"
     CDS             1055..2332
                     /locus_tag="VYN80_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1540375.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VYN80_000414"
     CDS             2408..3892
                     /locus_tag="VYN80_000414"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1540376.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VYN80_000415"
     CDS             3953..5233
                     /locus_tag="VYN80_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1540377.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5237..5857
                     /locus_tag="VYN80_000416"
     CDS             5237..5857
                     /locus_tag="VYN80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1540378.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSILYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7135
                     /locus_tag="VYN80_000417"
     CDS             6023..7135
                     /locus_tag="VYN80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1540379.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7458..7883
                     /locus_tag="VYN80_000418"
     CDS             7458..7883
                     /locus_tag="VYN80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1540380.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7894..8253
                     /locus_tag="VYN80_000419"
     CDS             7894..8253
                     /locus_tag="VYN80_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1540381.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8360..9004
                     /gene="pcp"
                     /locus_tag="VYN80_000420"
     CDS             8360..9004
                     /gene="pcp"
                     /locus_tag="VYN80_000420"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1540382.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9032..9889)
                     /locus_tag="VYN80_000421"
     CDS             complement(9032..9889)
                     /locus_tag="VYN80_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1540383.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10019..11161
                     /gene="tgt"
                     /locus_tag="VYN80_000422"
     CDS             10019..11161
                     /gene="tgt"
                     /locus_tag="VYN80_000422"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1540384.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11259..13076
                     /locus_tag="VYN80_000423"
     CDS             11259..13076
                     /locus_tag="VYN80_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1540385.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13229..14380
                     /gene="nagA"
                     /locus_tag="VYN80_000424"
     CDS             13229..14380
                     /gene="nagA"
                     /locus_tag="VYN80_000424"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1540386.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14543..15601
                     /locus_tag="VYN80_000425"
     CDS             14543..15601
                     /locus_tag="VYN80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1540387.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15752..16117
                     /locus_tag="VYN80_000426"
     CDS             15752..16117
                     /locus_tag="VYN80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1540388.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16192..17133
                     /locus_tag="VYN80_000427"
     CDS             16192..17133
                     /locus_tag="VYN80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1540389.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17081..18097
                     /locus_tag="VYN80_000428"
     CDS             17081..18097
                     /locus_tag="VYN80_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1540390.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRAGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18099..18425
                     /gene="comGC"
                     /locus_tag="VYN80_000429"
     CDS             18099..18425
                     /gene="comGC"
                     /locus_tag="VYN80_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1540391.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLQADGRITEEQAKAYKEYHDKNGVA
                     NRKVND"
     gene            18418..18822
                     /locus_tag="VYN80_000430"
     CDS             18418..18822
                     /locus_tag="VYN80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1540392.1"
                     /translation="MIKAFTMLESLLALSLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18827..19087
                     /locus_tag="VYN80_000431"
     CDS             18827..19087
                     /locus_tag="VYN80_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540393.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19050..19511
                     /locus_tag="VYN80_000432"
     CDS             19050..19511
                     /locus_tag="VYN80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1540394.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRFYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19489..19902
                     /locus_tag="VYN80_000433"
     CDS             19489..19902
                     /locus_tag="VYN80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1540395.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            20039..20632
                     /locus_tag="VYN80_000434"
                     /pseudo
     CDS             20039..20632
                     /locus_tag="VYN80_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20693..21646
                     /locus_tag="VYN80_000435"
     CDS             20693..21646
                     /locus_tag="VYN80_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1540396.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGETEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21697..22887
                     /locus_tag="VYN80_000436"
     CDS             21697..22887
                     /locus_tag="VYN80_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1540397.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23036..23407
                     /gene="rnpA"
                     /locus_tag="VYN80_000437"
     CDS             23036..23407
                     /gene="rnpA"
                     /locus_tag="VYN80_000437"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1540398.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23382..24206
                     /locus_tag="VYN80_000438"
     CDS             23382..24206
                     /locus_tag="VYN80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1540399.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24225..25211
                     /locus_tag="VYN80_000439"
     CDS             24225..25211
                     /locus_tag="VYN80_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1540400.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25262..25885
                     /locus_tag="VYN80_000440"
     CDS             25262..25885
                     /locus_tag="VYN80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1540401.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQCLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQTKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26026..27483
                     /locus_tag="VYN80_000441"
     CDS             26026..27483
                     /locus_tag="VYN80_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1540402.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDREKYYNGEVQEEA"
     gene            27506..27787
                     /locus_tag="VYN80_000442"
     CDS             27506..27787
                     /locus_tag="VYN80_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1540403.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27863..28348
                     /locus_tag="VYN80_000443"
     CDS             27863..28348
                     /locus_tag="VYN80_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1540404.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28365..29030
                     /locus_tag="VYN80_000444"
     CDS             28365..29030
                     /locus_tag="VYN80_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1540405.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29034..29897
                     /locus_tag="VYN80_000445"
     CDS             29034..29897
                     /locus_tag="VYN80_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1540406.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29899..30603
                     /locus_tag="VYN80_000446"
     CDS             29899..30603
                     /locus_tag="VYN80_000446"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1540407.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30780..32488
                     /locus_tag="VYN80_000447"
                     /pseudo
     CDS             30780..32488
                     /locus_tag="VYN80_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            32600..33691
                     /gene="ulaG"
                     /locus_tag="VYN80_000448"
     CDS             32600..33691
                     /gene="ulaG"
                     /locus_tag="VYN80_000448"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1540408.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33805..35781
                     /gene="tkt"
                     /locus_tag="VYN80_000449"
     CDS             33805..35781
                     /gene="tkt"
                     /locus_tag="VYN80_000449"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1540409.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35898..36197
                     /gene="yajC"
                     /locus_tag="VYN80_000450"
     CDS             35898..36197
                     /gene="yajC"
                     /locus_tag="VYN80_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1540410.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36243..36671
                     /locus_tag="VYN80_000451"
     CDS             36243..36671
                     /locus_tag="VYN80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1540411.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36673..37083
                     /locus_tag="VYN80_000452"
     CDS             36673..37083
                     /locus_tag="VYN80_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540412.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37383..40034
                     /gene="adhE"
                     /locus_tag="VYN80_000453"
                     /gene_synonym="adhC"
     CDS             37383..40034
                     /gene="adhE"
                     /locus_tag="VYN80_000453"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1540413.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12206 a         7219 c         9081 g        11756 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttatga aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttacgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgaattt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgtccg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag atttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagtct ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactac
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg atacttatcc
     2521 aaaggtagat ttgaactttg acaaattgaa agatgcttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agttgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cgaatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact cagactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggttaagact ggtgaaattg tagctggtga
     3181 aaaggttaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatt ggtttgccag ttggtaaatt aatctgtgct tcaaatgaca acaatgtttt
     3301 gacagacttc tttaaaacac gtgtctatga caaaaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgc ttgattttcc atcttttggg
     3421 aaataatgct gaaaagacaa ctgaacttat gaatgccttg aacacgcaag gacaatataa
     3481 gttgacagac tttgatgcag agattttgga cctctttgca gctgaatatg cgactgagga
     3541 agaaacggca gcagagatca agcgtgtttg tgagttagat tcttatatcg aggaccctca
     3601 tacagctgtt gcttcagcag tttataaaaa ataccaatcg gccactggag atgtaactaa
     3661 gacagtgatt gcttcaacag ctagtccata caagttccca gtagttgcag tagaagctgt
     3721 aactggaaaa gcaggtttaa cagactttga agccttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgaaatagct ccaattcgtc acaagacaac
     3841 agtggcagct gctgacatgc aagcagcggt tgaggcttat ttaggacttt aagacagagg
     3901 gagcaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctaggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattgtgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 ccagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtatagga atgtggtgta tccgcattgg
     5101 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgag
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattct
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agcatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatgata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtaacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagtt acaaagacag tgattgcttc
     6481 tgaagaagtg gcaccatcta cgggcacttc tgtcccagag gagcaaacga ccgaaacaac
     6541 tcgcccagtt gaagaagcaa ctcctcagga aacgactcca gctgagaagc aggaaacaca
     6601 agcaagccct caagctgcat cagcagtgga agtaactaca acaagttcag aagcaaaaga
     6661 agtagcatca tcaaatggag ctacagcagc agtttctact tatcaaccag aagagacgaa
     6721 aataatttca acaacttacg aggctccagc tgcgcccgat tatgctggac ttgcagtagc
     6781 aaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc tttaaagaag aaattgctaa
     6841 cttgtttggc attacatcct ttagtggtta tcgtccagga gacagtggag atcacggaaa
     6901 aggtttggct atcgacttta tggtaccaga acgttcagaa ttaggggata agattgcgga
     6961 atatgctatt caaaatatgg ccagccgtgg cattagttac atcatctgga aacaacgttt
     7021 ctatgctcca ttcgatagca aatatgggcc agctaacact tggaacccaa tgccagaccg
     7081 tggtagtgtg acagaaaatc actatgatca cgttcacgtt tcaatgaatg gataaacccg
     7141 acttgataac atcattttga cgaatgagat ctagctttcg tgatggaaag cgattctcgt
     7201 tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa ccacgtcagt tttatctgaa
     7261 acttcaaagc tgtgctttga gcaacctgcg actagcttcc tagtttgctt tttgattttc
     7321 attgagtatc aatttgaatg gaaaatggaa agttatcatc ttgtaatgag ttaagcaaca
     7381 ttcttgcaat ctattttact ttatatcaca attaattagt caaatattga taaatcaata
     7441 aaaagagagg ggaagaaatg ctagagattc aagatttact gtatcaactc cgcttgtctg
     7501 agcaagcgag tacgcaattg tttgaaaaaa ggcttgggat tagtttgaca cggtatcaga
     7561 ttttactgtt tttgctggag cattctcctt gtaaccaaat ggcggttcag gagcgtttga
     7621 aaattgatca ggctgctttg acacggcatt tcaaaatttt ggaaacggaa ggtttggtgg
     7681 agcgtcatcg taatcctgaa aatcagcggg aagtgttggt agaggctgcg aagtatgcca
     7741 aggagcagtt agtggtgaat ccccctctgc aacatatcag ggttaaggaa gagatagaaa
     7801 gtatcttaac agagtttgag agaacagaac tcagccgttt attaaataaa ttggttttgg
     7861 gtattgaaaa tatagaaatt taaggagaaa tagatgtcaa ttattttaac aacgatcgtt
     7921 gctttggagc atttttacat tttttatttg gaaagtattg ccacgcaatc agatgcgact
     7981 agtcgtgtat ttaatatgga aaaggaagaa ttggctcatc cgtcagtaag ttcattgttc
     8041 aaaaatcaag gaatttataa ggctctgcta ggagtctttc tcttgtatgt catttatttc
     8101 tcacagaatt tagaaattgt gactattttt gtcttatttg tgattggtgc tgcgacttac
     8161 ggctctttaa cagcggataa aaaaattatt ttgaaacaag gtggatcagc tattttggcc
     8221 ttgattagta ttttactctt taaatacact tgaaggtcga ttctaatctc gctaatcctt
     8281 tttaatccag aataagggaa atatgttata cttgttttta agaaaaaagt ctcattgaat
     8341 tggttttgag gagttagaaa tgaaagtatt agtgacaggt tttgagccct ttggagggga
     8401 aaagggcaat ccagctttgg aggccattaa aggtttacca gctgaaatcc atggtgctga
     8461 ggtccgttgg ctagaggtgc cgacagtttt tcacaaatct gctcaagtat tggaagaaga
     8521 gatgaatcgt tatcaacctg actttgtcct ttgtattggg caagctggtg gaagaactag
     8581 tttgacacct gaacgagtga ccattaatca agacgatgca tgcatttctg ataacgaaga
     8641 taatcaaccg attgaccgtc ccattcgccc agatggtgct tcggcctact ttagtagttt
     8701 gccgattaaa gcgatggttc aagctataaa aaaagagggc ttaccggcct ctgtttccaa
     8761 tacggcaggg acttttgtct gcagccattt gatgtatcag gctctctatt tggtagaaaa
     8821 gaaatctcca tatgttaagg caggttttat gcatattcct tatatgatgg aacaggtggt
     8881 gaacagaccg actactccag ctatgagttt agtggatatt cggcgaggga tagaagcagc
     8941 aatcggcgct ataatagaac atggagatca ggaactcaag ttggtaggcg gagaaactca
     9001 ttgatagaaa aaagcttgag gggaaaaacc ttcaagcttt tggacgtttt cgggccaata
     9061 ctgctcggta aaacataatt ttagtgcatt ggatataagg taggagtgaa aaactagcaa
     9121 tgccaaaggt aatccaattg aggaagtacc aaggaagaag ctgtaaatct aggacaaagt
     9181 gctggaactt gtagcccttc ataaaggaac ggctagtttt taggattcgt cttggtggga
     9241 cctgtcctag gtctagacta taacagagaa gaaattccac ctgtgaatag gcataatact
     9301 gtggaatata gaggatattt cctacaatga tcaagatgag acttgcaaga aagtagagtc
     9361 caaagaccat gaggaaacgc tcggtttcaa ctgatgagag atctagattt ggaaactcag
     9421 gatgtagggt gacgaatttt ttggctaaaa agctactata aaagaggagg taaatcccaa
     9481 gtaaattagg gatactccat aaaaagagat agaaacgttt gagaagtagg gtcaaaaagg
     9541 tttgagaaaa gcgctcctca tcaaagagag ctaggctgtt ttttacagat ggctccgttt
     9601 tagaatcttt catgagtgtc agtgttgcat agacggaact ggtcaaaaga atagtcccga
     9661 taaaggagac tagtagagga aagaggtagg tttgaagtat ttggccaagt atgctgaaaa
     9721 atggctgttc taaaacagtc ccgtggatcc gagataaggg attaagaaaa ccagataaga
     9781 tgaccagcat actgggaagg atatagagga gaaagagacg gggggtgtca gcctgaaaat
     9841 gttttgactc ctgacgaatt gtttttaaat caatttttgg atagttcatt ctcttattat
     9901 accatagttc ttatacatag ttcgtgacag ttcctacttt ttttgataaa atcatacagt
     9961 gtgtccttgg gcacactgta tgaactggga ctgtctttcc cagcttcgga ggtaaaaaat
    10021 gtcagattca ccaatcaaat atcgtttgat taagaaagaa aaacacacag gagctcgtct
    10081 gggagaaatc atcactcccc acggtacctt tccgacacct atgtttatgc cagttgggac
    10141 acaagccact gtcaaaactc agtcacctga agaattgaag gagatgggtt cgggaattat
    10201 cctatcaaac acctatcatc tctggcttcg ccctggagat gaactcattg cacgcgctgg
    10261 tggtctccac aagttcatga attgggacca gcctatcttg acagatagtg gtggttttca
    10321 ggtttattct ttagcagata gccgtaatat cacagaagaa ggagtaacct ttaaaaatca
    10381 tctaaatggt tctaagatgt tcctatcccc agaaaaagcc atctctattc agaataatct
    10441 gggttcagac atcatgatgt cctttgatga atgtcctcag ttttatcaac cttatgacta
    10501 cgttaagaaa tcgatcgagc gtaccagccg ttgggctgag cgtggtttga aggctcaccg
    10561 tcgtccacat gaccaaggtt tgtttggaat tgtgcaaggt gcaggatttg aagaccttcg
    10621 ccgccaatca gctcatgatc ttgtcagcat ggatttctca ggctactcta tcggtggttt
    10681 ggcagtggga gaaacccatg aagagatgaa tgcggtcttg gactttacaa ctcaactgct
    10741 acctgaaaat aaacctcgtt atctgatggg tgtgggagcg ccagatagct tgatcgatgg
    10801 ggtcattcgt ggggtggata tgtttgactg tgtcttaccg actcgaattg ctcgtaacgg
    10861 gacttgtatg accagtcaag gacgtttggt tgtgaaaaat gcccagtttg ctgaggactt
    10921 tacgccactg gatcctgagt gtgactgcta cacatgtaat aactatacac gcgcttacct
    10981 tcgtcacctg ctcaaggctg atgaaacctt tggtatccgc ttgactagct accacaatct
    11041 ttacttcttg cttaacctga tgaagcaagt gcgacaagcc atcatggatg acaatctctt
    11101 ggaattccgt gagtattttg tggaaaaata tggctataat aagtcaggac gtaatttcta
    11161 aaatggaatt gatataaaaa aatcctaagt tttctcttag gatttttctt ctttttttga
    11221 tagaataaag tgtacaatga aaggaagaat aaactcgtat gcgcattaaa tggttttcct
    11281 tgattaggat tataggttta cttttggtac tcttgtatca cttctttcag acgatctttc
    11341 ctggaggatt tttcggggta gatgtctttt tcacattttc aggtttcctg attacagctc
    11401 tactcattga agaattttct aagaacaatg agattgattt gataggattt tttagaagac
    11461 gcttttatcg gattgtgcca cctgtggttt tgatggtctt ggtgaccatg ccttttactt
    11521 tcttggttcg ccaagactat gttgctggaa ttggtggcca gattgcgggc gtcttaggct
    11581 ttatgaccaa cttctatgaa ctcctaacag gtgggagtta tgaatctcag ttcattcctc
    11641 atttgtttgt tcataattgg agtttggcag ttgaggttca ctactatatt ctttggggat
    11701 tggcagtttg gttcttatcc aaacaagcta aatcaaatgg tcagttgaag gggatggtct
    11761 ttctcttatc tgctgctgcc ttcttgatca gtttcttctc catgtttatt ggtagttttc
    11821 tagtgacctc ttattcctct gtttatttct ccagtttaac tcatgtctat ccattctttt
    11881 tgggaagtat gttagcaact attgtaggtg ttcgtcagac gacttccctc gtcaagcagt
    11941 tggataaaat ctgggattta cgaaagactt tggtagtttt tggaggaggc tttggtttcc
    12001 tagttctttt gactttcttt gtcaaattca cttatctttt tgcctatctt atcggcttct
    12061 tgcttgccag tcttgcagct cttgccatga ttctggcggc gcgtgtctta catgaaaaga
    12121 cacatcatat acaggagccg aagattatca gctttttagc ggatactagc tatgcggttt
    12181 atcttttcca ttggcctttc tatatcattt tctcacagtt gacatcaaat cttcttgctg
    12241 tattactgac tctgatttgt tcttatggct ttgccagtct gtcattctat gtattggaac
    12301 cttggattgc aggcaagaac acacctattt tacaaactct tcgtcccctg ccttatattc
    12361 acgcaattct tgtagcaggt acaggaatct tgaccatcat tgtctgcacg gtgaccttgt
    12421 tggcaccaca agtgggagcg tttgagacag acttgactgt caatggcttg aagcaagctg
    12481 caacaaatat tggccagacc aaggtgatgg cagaacgggc agatgcaaac agtttgggaa
    12541 ttgctgatgg cactatgtta attggtgact cagtggcttt aagggcaaat acagcactac
    12601 agacagctct tcctggagca cagattaacg cgcaggtcag cgtaacaacc aagaccgcaa
    12661 atgaaatcat gctaaataat agccagaata aatttttacc taagacggtg gtcattgcga
    12721 ctggggtaaa taatcctgag aattacaagg atgactggga cagtatcgtg aaaaatcttc
    12781 ctaagggaca ccatatgatt ttggtgactc cttatgaggg agataagaca aaagagacct
    12841 atgccatcgt tgagaaggct gctgcctata tgagagaatt ggcagagaag acaccttaca
    12901 ttacgatagc agattggaat caagttgcga aagagcatcc agaaatttgg gctggaacag
    12961 accaggttca tttcgggagt gagagtagca ctatcgaagc aggagcaaaa ttgtatgcag
    13021 atacgattgc cacagctttg cagacagctc aagacaagcc ggttaaatca aaataacttg
    13081 tattaaaaaa gagaagagtt ggagaaatcc agctctttta aaatatctcc aaaaaatagg
    13141 tctataccat ttacaaataa aaaagaaagg tttataatgt aattgacata ataaattgta
    13201 gaatcaatct tttaaggagg ttaacattat gcctaactat attaaagcgg atcagttttt
    13261 ctacccacac ggagttcgtc gaggtggtta cttggaactt gtggacggca agtttgggaa
    13321 acatgtagag cagattcctg aaggggctga ggtgattgac tatacaggtt atagcattgc
    13381 cccaggtctt gtggatactc atattcatgg atatgcaggt gtagatgtga tggacaacaa
    13441 cattgaaggt acattgcata ctatgagtga aggacttctt agtaccggtg ttaccagttt
    13501 cttacccaca actttaacag ccacttatga gcaattgctt gcagtcactg aaaatcttgg
    13561 aaaccattat aaagaagcaa caggtgctaa gattcgtggg atttattatg aaggtccata
    13621 tttcacagaa acttttaagg gggcacaaaa tccaacttat atgagagacc cgggtgttga
    13681 ggagtttcat tcttggcaaa aagcggcaaa tggcttgctt aataaaattg cccttgcacc
    13741 agaacgtgat ggggtggaag actttgttcg tacagttacg ggcgaaggtg tgacggttgc
    13801 tcttggacat tcaaacgcga cttttgatga agccaaaaaa gcagtcgatg ctggagcgag
    13861 tgtttgggtg catgcctaca atggaatgcg tgggttgact caccgtgaat tgggtatggt
    13921 tggagccatg taccaattgc cacataccta tgcagagttg atctgtgatg gtcaccacgt
    13981 agatccaaag gcttgcgaaa ttcttatcaa acaaaaagga acagaaaaca tcgctcttat
    14041 cacagactgt atgacagctg ggggattgga agacggagat tatatgttgg gagaattccc
    14101 agttgtcgtt gcaaatggaa ctgcacgcct caaatcgaca ggtaacttgg caggttctat
    14161 cctcaaactc aaagatggtt tgaagaatgt ggtcgaatgg ggaattgcga atccgcatga
    14221 agcagtcatg atggccagct tcaacccagc taaatccgtt cacatcgatg acgtctgtgg
    14281 ccaaatccgt gaaggctacg acgctgactt catcgtatta gataaagatt tggaattggt
    14341 agcaacctat ctagatggcg taaaacgtta tcaagcataa gagagaaggc agaagttaga
    14401 gactagcttc tgctttttta tacagggtac tttactggta aataaaaagt taaaaaaatc
    14461 acaaaaaaag cttgaagaaa caaagaaaaa ggcttacaat tgaactataa atattacaaa
    14521 taaaaaaacg gaggagtgct ttatgaaagc ctatacttat gttaaaccag gacttgcttc
    14581 ttttgttgat gtagacaaac cagttattcg caagccaaca gacgctattg tgcgtattgt
    14641 aaaaaccact atttgtggaa cagacctcca tattatcaaa ggggatgttc ctacttgcca
    14701 aagtggtacc attcttggcc acgaagggat tgggattgtt gaagaagttg gggaaggagt
    14761 ttccaacttc aaaaaaggcg acaaggtctt gatttcttgc gtctgtgcct gtggtaaatg
    14821 ctactactgt aaaaaaggaa tttatgccca ctgtgaagac gaagggggct ggattttcgg
    14881 tcacttgatt gatggtatgc aggctgaata tctacgtgtc cctcatgcag ataatactct
    14941 ttaccatact ccagaagact tgtcagatga agctttggtt atgctgtcag acattctgcc
    15001 tactggatat gaaattggtg tcttaaaagg gaaagtagaa cctggttgca gcgtagccat
    15061 tattggttca ggtccagttg gattggctgc tcttttaaca gcccaattct attcaccagc
    15121 taaattgatt atggtagacc tagacgataa ccgcttggaa actgccctat cattcggtgc
    15181 gactcataag gttaattctt cagaccctaa aaaagccatt aaagaaattt atgatttgac
    15241 agatggtcgt ggtgtggatg tcgctatcga agctgtaggg attcctgaaa catttgattt
    15301 ctgtcaaaag attatcggtg tagacggaac ggttgccaac tgtggtgtgc atggtaaacc
    15361 agttgaattc gatttagata aactttggat tcgcaacatc aatgtaacaa ctggtttggt
    15421 atctacaaat acgactccac aattgttgaa agcacttgaa agtcataaga ttgaaccgga
    15481 aaaattggta actcactatt tcaaactcag tgaaattgaa aaagcctacg aagtcttcag
    15541 taaggcagca gaccaccatg ccattaaggt cattatcgaa aacgatatct cagaagccta
    15601 agtagtaaaa atatttttgt acataagtaa atagaaattc agtcatccat cagatggttg
    15661 gattttttat caaaaaatta agaaatgagc atatttcttt ccttgtctgg tggaattggt
    15721 tataatatac ggtacaaagg aatgaatgaa tatgtatcgt gttatagaaa tgtacggaga
    15781 ttttgaaccg tggtggttct tagaaggttg ggaagaagat attgtagcaa gtagaaaatt
    15841 tgaccagtat tatgatgctc tcaaatacta caaaacttgc tggtttagat tggaacaaga
    15901 atcgcctctt tataaaagta gaagcgactt gatgaccatt ttttgggacc cggaagacca
    15961 acgctggtgt gatgaatgtg atgagtattt acaacaatac cattctttgg ctcttttgca
    16021 ggatgagcag gttatcccag acgaaaaact acgctcaggc tatgaaaaac aaaccagtca
    16081 ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga aaagtaactt tttggagttg
    16141 ctttttttat ttttctaacg ctttgcgaat agtataggtg aggaggtaag tatggttcaa
    16201 gaaattgcac aagaaatcat tcgttcggct cggaaaaaag gggcgcaaga catttatttt
    16261 gtccccaagt tagatgccta tgagcttcat atgagggtag gagacgagcg ctgtaaaatt
    16321 ggtagctatg attttgaaaa gtttgcagcc gttatcagtc actttaagtt tgtggcgggt
    16381 atgaatgtgg gagaaaaaag acgtagtcaa ctgggttcct gtgattatgc ctatgaccag
    16441 aagatggcgt ctctacgttt atctactgta ggcgattatc gggggcatga gagtttggtt
    16501 atccgtttgt tgcacgatga ggagcaggac ttgcattttt ggtttcagga tattgaagaa
    16561 ttaggcaagc agtacaggca acggggactc tatctttttg ctggtccggt tgggagtggt
    16621 aagacgacct tgatgcatga attgtccaag tcactcttta aaggacagca agttatgtcc
    16681 atcgaagatc ctgtcgaaat caagcaggac gacatgcttc agttgcagtt gaacgaagca
    16741 atcggcctaa cctatgaaaa tctaatcaaa ctttccttgc gtcatcgacc agatctcttg
    16801 attatcggag aaattcgtga cagcgagacg gcgcgtgcag tggtcagagc tagtttgaca
    16861 ggtgcgacag tcttttcaac cattcacgcc aagagtatcc gaggtgttta tgagcgtctg
    16921 ctggagttgg gtgtgagtga agaagaattg gcagttgttc tgcaaggagt ctgctaccag
    16981 agattaatcg ggggaggagg aatcgttgac tttgcaagca gagattatca agaacaccaa
    17041 gcagccaagt ggaatgagca aattgaccag cttcttaaag atggacatat cacaagtctt
    17101 caggctgaga cggaaaaaat tagctacagc taagcaaaaa aatatcatca ccctatttaa
    17161 caatctcttt tctagcggtt ttcatctggt ggagaccatc tcctttttag ataggagtgc
    17221 tttgttggac aagcagtgtg tgacccagat gcgtgcgggc ttgtctcagg ggaaatcatt
    17281 ctcagaaatg atggaaagtt tgggatgttc aagtgctatt gtcactcagt tatccctagc
    17341 tgaagtccat ggcaatctcc acctgagttt gggaaagata gaagagtatc tagacaatct
    17401 ggccaaggtc aagaaaaaat taattgaagt agcgacctat cctttgattt tgctgttttt
    17461 tcttctctta attatgctgg ggctacggaa ttacctgctc ccacaactgg atagtagcaa
    17521 tattgccacc cgaattatcg gtaatctgcc acaaattttt ctaggcatgg tagggcttgt
    17581 ttccgtgctt gcccttttag cactaacttt ttataaaaga agttctaaaa tgagtgtctt
    17641 ttctatctta gcacgccttc cctttattgg aatctttgtg cagacctact tgacagccta
    17701 ttatgcacgt gaatggggga atatgatttc acagggaatg gagctgacgc agatttttca
    17761 aatgatgcag gaacaaggtt cccagctctt taaagaaatc ggtcaagatc tggctcaaac
    17821 cctgaaaaat ggccgtgaat tttctcagac gataggaacc tatcctttct ttaggaagga
    17881 attgagtctc atcatagagt atggggaagt taagtccaag ctgggtagtg agttggaaat
    17941 ctatgctgaa aaaacttggg aagccttttt tacccgagtc aaccgcacca tgaatttggt
    18001 gcagccactg gtttttatct ttgtggcact gattatcgtt ttactttatg cggcaatgct
    18061 catgcccatg tatcaaaata tggaggtaaa tttttaaaat gaaaaaaatg atgacattct
    18121 tgaaaaaagc taaggttaaa gcttttacat tggtggagat gttggtggtc ttgctgatta
    18181 tcagcgtgct tttcttgctc tttgtaccta atctgaccaa gcaaaaagag gcagtcaatg
    18241 acaaaggaaa agcagctgtt gttaaggtgg tggaaagcca ggcagaactt tatagcttgg
    18301 aaaagaatga agatgctagc ctaagcaagt tacaagcaga tgggcgaatc acggaagaac
    18361 aggctaaagc ttataaagaa taccatgata aaaatggagt agcaaatcgt aaagtcaatg
    18421 attaaggcct ttaccatgct ggaaagtctc ttggctttga gtcttgtgag tatccttgcc
    18481 ttgggcttgt ccggctctgt tcagtccact tttgcggcag tagaggaaca gattttcttt
    18541 atggagtttg aagaactcta tcgggaaacc caaaaacgca gtgtagccag tcagcaaaag
    18601 actagtctga acttagatgg gcagacgctt agcaatggca gtcaaaagtt gccagtccct
    18661 aaaggaattc aggccccatc aggtcaaagt attacatttg accgtgctgg gggcaattcg
    18721 tccctggcta aggttgaatt tcagaccagt aaaggagcga ttcgctatca attatatcta
    18781 ggaaatggaa aaattaaacg cattaaggaa acaaaaaatt agggcagtga ttttactgga
    18841 agcagtagtc gctctagcta tctttgccag cattgcgacc ctccttttgg gacaaattca
    18901 aaaaaatagg caagaggaag caaaaatctt gcaaaaggaa gaagtcttga gggtagctaa
    18961 gatggccctg cagacggggc aaaatcaggt aagcatcaac ggagttgaga ttcaggtatt
    19021 ttctagtgaa aaaggattgg aggtctacca tggttcagaa cagttgttgg caatcaaaga
    19081 gccataaggt caaggctttt accttgttag aatccctgct tgccctcatt gtcatcagtg
    19141 ggggattact cctttttcaa gctatgagtc agctcctcat ttcagaagtt cgctaccagc
    19201 aacaaagcga gcaaaaggag tggctcttgt ttgtggacca acttgaggta gaattagacc
    19261 gttcgcagtt cgaaaaagtg gaaggcaatc gtttctatat gaagcaagat ggcaaggaca
    19321 tcgccatcgg taagtcaaag tcagacgatt ttcgtaaaac cgatgctagt ggacgggggt
    19381 atcagcctat ggtttatggc ctcaaatcag ctcagattac agaggacaat caactggttc
    19441 attttcgttt ccagtttcaa aaaggcttag aaagggagtt catctatcgt gtggaaaaag
    19501 aagaaagtta aggcaggtgt tctcctctat gcagtcacca tggcagctat ctttagtctt
    19561 ttgttgcaat tttacttgaa ccgacaagtc gcccactatc aagactatgc tttgaataaa
    19621 gaaaaattgg ttgcttttgc catggctaaa cgaaccaagg ataaagccga gcaagaaagt
    19681 ggggaacagg tctttaatct aggtcaggta agctatcaaa acaagaaaac aagcttggtg
    19741 acgactgttc gtacggataa gagtcaatat gagtttctgt ttccttcagt caaaatcaaa
    19801 gaagagaaaa gagataaaaa ggaagaggta gcgactgatt caagcgaaaa agcggagaag
    19861 aaaaaatcag aagagaagcc tgaaaagaaa gagaattcct agtcaattca actataatgc
    19921 gttgaatcca aaatagtcca ctgtagtttc tagaaaattg ctggaaatgg atgttaagct
    19981 ccaattcatt tgtttatatc ttatttcagt ttactatact ttgtgctaaa ctaaagatat
    20041 gaaacatgat tttaaccaca aagcagaaac tttcgattcc cctaaaaaat cttcctcgca
    20101 aacttggtat gtcaagcagc cgagaaacag attgatcttc tatcagacaa agaaatttta
    20161 gatttcggtg gtggcacgga tctattagcc ttgcccctag cccctagcca agcaggctaa
    20221 gtcagtcact cttgtagaca tttctgagaa aatgttggag caagctcgtt tgaaagtgga
    20281 gcagcaagca atcaagaata tccagttttt ggagcaagat ttaccgaaaa atcccttgga
    20341 gaaagagttt gattgccttg ctgttagtcg ggttcttcat catatgcctg atttggatgc
    20401 ggctctctca ctgtttcatc aacatttgaa ggaagatggg aaactcatca ttgctgattt
    20461 taccaagaca gaagctaatc atcatggatt tgatttagct gaactggaaa acaagctaat
    20521 tgagcatggt ttttcatctg tgcatagtca gattctctat agcgctgaag acctgtttca
    20581 aggaaatcac tcagaattct ttttaacagt agcccaaaaa tcactcgcct agtcagggag
    20641 tgatttttct ataaggatgg aaaaaagaag ggaaatttgg taagatagta atatggattt
    20701 tgaaaaaatt gaacaagcct atacctattt actagagaat gtccaagtca tccaaagtga
    20761 tttggcgacc aacttttatg acgccttggt ggagcaaaac agcatctatc tggatggtga
    20821 aactgagcta aaccaggtca aagacaacaa tcaggccctt aagcgtttag cactacgcaa
    20881 agaagaatgg ctcaagacct accagtttct cttgatgaag gctggggaaa cagaaccctt
    20941 gcaggccaat caccagttta caccagatgc cattgctttg cttttggtgt ttattgtgga
    21001 agagttgttt aaagaggagg aaattactat cctcgaaatg ggttctggga tgggaattct
    21061 aggcgctact ttcttgacct cgcttgataa aaaggtggat tacttgggaa tggaagtgga
    21121 tgatttactg attgatctgg cagctagcat ggcagatgta attggtttgc aggctggctt
    21181 tgtccaagga gatgccgttc gtccacaaat gctcaaagaa agcgatgtgg tcatcagtga
    21241 cttgcctgtc ggctattatc ctgatgatgc cgttgcgtcg cgtcatcaag ttgcttctag
    21301 ccaagaacat acttacgccc atcacttgct catggaacaa gggtttaagt acctcaagtc
    21361 agatggatat gctatttttc tagctccgag tgatttgttg accagtcctc aaagtgattt
    21421 gttgaaagtc tggttgaaag aggaggcaag tctggttgct atgattagtt tacctgaaaa
    21481 tctctttgct aatgccaaac aatctaagac tatttttatc ttacagaaga aaaatgaaat
    21541 agcagtagag ccttttgttt atccacttgc tagcttgcaa gatgcaagtg ttttaatgaa
    21601 atttaaagaa aattttcaaa aatggactca aggtactgaa atataaaata gattttgtta
    21661 taatagttga aaacgcttaa aaaggggtat catgttatga caaaaacaat tgcaatcaat
    21721 gcaggaagtt caagtttgaa atggcaatta tacttaatgc cagaggaaaa agtgttagcc
    21781 aaaggtttga ttgaacgtat cggtttgaaa gattcaattt caactgtaaa atttgacggc
    21841 cgttctgaac aacaaatttt ggatatcgaa aatcatacac aagccgttaa aattttattg
    21901 gatgacttga ttcgtttcga tattatcaag gcttatgacg agattacagg tgttggacat
    21961 cgtgttgttg ctggtggaga atatttcaaa gaatcaacag ttgttgaggg agatgtttta
    22021 gaaaaagttg aagagttgag tttgttggct cctctacaca acccggccaa tgcagcaggt
    22081 gttcgtgcct tcaaggaatt gttgccagac attaccagtg tagttgtttt tgatacttcc
    22141 ttccacacaa gtatgccaga gaaagcttat cgctaccctc taccaacaaa atattacaca
    22201 gaaaacaagg ttcgtaaata cggtgctcat ggtacaagtc accagtttgt agcaggagaa
    22261 gctgcaaaac tcttgggacg tccattagaa gacttgaagt taattacctg tcatattggt
    22321 aacggaggct caattacagc tgtgaaagcc ggcaaatctg tagacacttc tatggggttc
    22381 actcctcttg gtggtattat gatgggaacg cgtacagggg atattgatcc agctatcatt
    22441 ccttatttaa tgcaatatac agaggatttt aacacaccag aagatatcag tcgtgttctt
    22501 aaccgtgaat caggtctttt gggagtttct gctaattcta gcgatatgcg cgatatagaa
    22561 gcagctgtag cagaagggaa tcacgaggct agcttggctt atgaaatgta tgttgaccgt
    22621 atccaaaaac atatcggtca gtaccttgca gtgctaaatg gagcagatgc cattgttttc
    22681 acagcaggtg tcggtgaaaa tgcagagagt ttccgtcgtg atgtaatctc agggatttcg
    22741 tggtttggtt gtgatgttga tgatgaaaag aatgtctttg gcgttacagg agacatctca
    22801 acagaggcag ctaaaatccg tgtcttggtt attccaacag atgaagaatt agtcattgcc
    22861 cgtgacgttg aacgcttgaa aaaataagtg aaactaaaaa aatattcaat acaaggagtt
    22921 gggaaagtta tttttccagc ttctttttct gatgaaattg tccaaaacct tgctatgatt
    22981 ggcttttttg aaaaatatgg tataatagta gtaatttaat agatggagtt gagttttgaa
    23041 gaaaaacttt cgtgtaaaaa gagagaaaga ttttaaggcg attttcaagg aggggacaag
    23101 ttttgctaat cgcaaatttg tggtctacca attagaaaac cagaaaaacc attttcgagt
    23161 aggtctatca gttagcaaaa aactggggaa tgccgtcact agaaatcaaa ttaagcgacg
    23221 gattcggcat attatccaga atgcaaaagg gagtctggta gaagatgtcg actttgttgt
    23281 cattgctcga aaaggagtcg aaaccttggg atacgcagag atggagaaaa atctactcca
    23341 tgtattaaaa ttatcaaaga tttaccggga aggaaatggg agtgaaaaag aaactaaagt
    23401 tgactagttt gctaggactg tctctgttaa tcatgacagc ctgtgcgact aatggggtaa
    23461 ctagcgatat tacagccgaa tcggctgatt tttggagtaa attggtttac ttctttgcgg
    23521 aaatcattcg ctttttatcg tttgatatta gtatcggagt ggggattatt ctctttacgg
    23581 tcttgattcg tacagtcctc ttgccagtct ttcaggtgca aatggtggct tctaggaaaa
    23641 tgcaggaagc tcagccacgc attaaggcgc ttcgagaaca atatccaggt cgagatatgg
    23701 aaagcagaac caaactagag caggaaatgc gtaaagtatt taaagaaatg ggtgtcagac
    23761 agtcagactc tctttggccg attttgattc agatgccggt tattttggcc ctgttccaag
    23821 ccctatcaag agttgacttt ttaaagacag gtcatttctt atggattaac cttggtagtg
    23881 tggatacaac ccttgttctt ccgattttag cagcagtatt caccttttta agtacttggt
    23941 tgtccaacaa agctttgtct gagcgaaatg gcgctacgac tgcgatgatg tatgggattc
    24001 cagtcttgat ttttatcttt gcagtttatg cgccaggtgg agtcgcccta tactggacag
    24061 tgtctaatgc ttatcaagtc ttgcaaacct atttcttgaa taatccattc aagattatcg
    24121 cagagcgcga ggccgtagta caggcacaaa aagatttgga aaatagaaaa agaaaagcca
    24181 agaaaaaggc tcagaaaacg aaataaataa ggaggaatct ggtaatggta gtatttacag
    24241 gttcaactgt tgaagaagca atccagaaag gattgaaaga attagatatt ccaagaatga
    24301 aggctcatat caaagtcatt tctagggaga aaaaaggctt tcttggtcta tttggtaaaa
    24361 aaccagccca agtggatatt gaagcgatta gtgaaacgac tgttgtcaaa gcaaatcaac
    24421 aggtagtaaa aggcgttccg aaaaaaatca atgatttgaa cgagcctgtg aagacggtta
    24481 gtgaagaaac cgttgacctt ggtcatgtgg ttaatgctat taaaaaaata gaggaagaag
    24541 gtcaaggtat ttctgatgaa gtcaaggctg aaatcttaaa acatgaaaga catgccagca
    24601 ctatcttaga agaaactggt cacattgaga ttttaaatga acttcaaatc gaggaagcga
    24661 tgagggaaga agcaggcgct gatgaccttg aaactgagca agatcaaact gaaaatcaag
    24721 acttgaaaga gatgggcttg aaggtcgagc aaagttatga tattgcccag gtggctacgg
    24781 atgtgactgc ctatgttcaa gcgattgtgg atgacatgga tgttgaagct acactttcaa
    24841 atgattataa ccgtcgtagc atcaatctac aaattgacac caacgaacca ggtcgtatta
    24901 tcggctacca tggtaaagtc ttgaaggcct tgcaactgtt ggctcaaaat tatctttaca
    24961 accgctattc caaaaccttc tacgttacaa tcaatgtcaa tgattatgtc gaacaccgtg
    25021 cagaagtctt gcagacctat gcgcaaaaat tggcgaatcg tgttttggaa gaaggtcgca
    25081 gtcataaaac agatccaatg tcaaatagcg aacgcaagat tatccatcgt attatttcac
    25141 gtatggatgg cgtgactagt tactctgaag gtgatgagcc aaatcgctat gttgttgtag
    25201 atacagaata agtaaaatca gggttgacct gattttttgc tagttagagg aggttaaact
    25261 gatgttgaat aagataagag actatctaga cttttctggt ttgcagtacc gtagtcctga
    25321 taaagcggga gcagagcgag agaagatgct ggcgttccgc cacaaaggac aagaggcccg
    25381 aaaggctttt acagaactgg ccaaagcctt tcaagcaagc catccagaat ggcaactcca
    25441 acagactagt cagtggatga atcaggccca gtgtttgaga ccacattttt gggtttatct
    25501 acagagagac ggacaagtga cagaacctat gatggcctta cgtttgtatg ggacatctac
    25561 tgactttgga atttctttgg aagtcagttt catcgaacgt aagaaggatg agcaaacact
    25621 gggcaagcag accaaagttt tagacattcc aaccgttaaa gggatttatt atctaaccta
    25681 ctctaatggt caaagtcaac ggtgggaggc gaatgaagaa aagcgtcgta ctttacgcga
    25741 gaaggtgaga agtcaagaag ttcgaaaagt tttagtgaag gtagatgttc ctatgacaga
    25801 aaattcgtct gaagaagaaa tcgtagaagg cttattgaag tcttattcta aaattcttcc
    25861 ctattatcta gctacgagaa aataagataa tttgtaaaac atcataaatc atcataaatc
    25921 atacagtcca agagtgaaca gtccgctgtg taattcttgg tctttttgtt tgcgctttcg
    25981 cattatataa taaacttaca aaaacaattc aaaaggagaa caattatgga agtcgtttca
    26041 agtgttctaa attggttttc tagcaatatt ttgcagaatc ccgcattttt cgtaggttta
    26101 ttggtgttga taggatatgc acttttgaaa aaacctgccc atgacgtttt ttcagggttt
    26161 gttaaagcaa cagtagggta tatgttgctt aacgtgggtg ctggtggttt ggttacaacc
    26221 tttcgtccaa tcttagcagc tcttaactac aaattccaaa ttggtgcagc ggttatcgac
    26281 ccttactttg gacttgctgc agcaaacaac aaaattgcag cagagtttcc agattttgtt
    26341 ggaactgcaa ctacagctct attgattggt tttggaataa atatcttgct cgtagctctt
    26401 cgaaagatta cgaaggtaag aaccctcttt attactggtc acatcatggt acaacaagct
    26461 gcaacagtat ctcttatggt tctattctta gtaccacaat tgcgcaatgc ttacggtaca
    26521 gcagcgattg gtatcatctg tggactttac tgggcagtta gttcaaatat gactgttgag
    26581 gcaactcaac gcttgactgg tggtggcgga tttgcgattg gtcaccaaca gcaatttgca
    26641 atctggtttg tagataaagt agcaggacgc tttggtaaga aagaagaaag tttagacaat
    26701 cttaaattac ctaagttcct ctcaatcttc cacgatacag ttgttgcatc tgctaccttg
    26761 atgctcgtat tcttcggagc cattctttta atcttgggtc cagacattat gtctaataaa
    26821 gaagtcatca cttcaggaac tctattcaat cctgctaaac aagatttctt tatgtacatt
    26881 atccaaacag cctttacctt ctcagtttac ttgttcgttt tgatgcaagg tgtccgaatg
    26941 ttcgtatctg agttgacaaa cgccttccaa ggtatttcaa acaaattgtt gccaggttca
    27001 ttcccagcgg ttgacgttgc agcttcttat ggatttggtt ctccaaatgc tgtcttgtca
    27061 ggatttacct ttggtttgat tggtcaattg attacaattg tcttgctcat cgtctttaaa
    27121 aatccgattc ttattattac aggatttgta ccagtgttct ttgacaatgc agccattgcg
    27181 gtctacgctg ataaacgcgg cggatggaaa gcggctgtta tcctttcctt tatatcaggt
    27241 gtccttcaag ttgctctagg agctctttgt gtggcccttc tcgatttggc atcttatggt
    27301 ggctaccatg gaaatatcga ctttgaattc ccatggcttg gatttggata tatcttcaaa
    27361 taccttggta ttgttggtta tgtacttgtg tgtctcttct tgcttgttat tcctcaactt
    27421 caatttgcca aagcaaaaga tagagagaaa tattacaacg gtgaagttca agaagaagct
    27481 tagtatctag aaaaggagaa ataaaatggt taaagtatta gcagcgtgcg gaaatggaat
    27541 gggttcatca atggttatca agatgaaggt tgaaaatgct ctccgtaagc ttaatcaaac
    27601 agattttaca gtcaattcat gcagtgtcgg tgaagctaaa ggtttagcag taggatatga
    27661 catcgtaatc gcttctcttc atttgattca agaattggaa gggcgaacta atgggaagtt
    27721 aattgggctt gataacttga tggatgataa agaaatcacc gaaaaactca gtcaagcact
    27781 acagtaaaag gttggagggg ctggacagaa actgagagtt atcgtttctg tccttctccc
    27841 tctttaaata aaggaggcag atatgaattt aaaacaagct ttaattgaca atgactcgat
    27901 ccgactaggt ttagaggcta acaattggaa agaagcagtc aaggtagcag tagatccctt
    27961 aattgaaagt ggggcaattt tgccagagta ttacgatgct atcattgaat cgactgaaga
    28021 gtatgggcct tactatatct tgatgccagg tatggctatg ccccacgcta gacctgaagc
    28081 tggtgtgcaa agtgatgcct tttcattgat taccttacaa aatcctgttg tattttcaga
    28141 tgggaaagag gtatctgttt tgttggcact agcagcaaca agttcaaaaa ttcacacaag
    28201 tgtagccatt ccacaaatta ttgccctgtt tgaattagaa gattctattg cacgtttaca
    28261 ggcttgccag actaaagaag atgtcttggc tatgattgaa gaatctaagg atagccctta
    28321 tctcgaagga ttggatttgg aaagttagaa agaggaataa agaaatgaca aaaagaatac
    28381 ctaatttaca agttgcatta gaccattcag acttgcaagg agcgattaaa gcagctgttt
    28441 ctgttggtca ggaagtagat attatcgaag ctggaactgt ttgcttgctt caagttggaa
    28501 gtgaactggc tgaagtcttg cgtagccttt tcccagataa gattattgtg gcagacacaa
    28561 aatgtgctga tgctggtgga acagttgcta aaaataatgc ggttcgtgga gcagactgga
    28621 tgacttgtat ctgttgtgca accatcccta ctatggaagc agctctaaag gctatcaaga
    28681 ctgaacgagg agaacgaggc gaaatccaga tcgagcttta tggcgattgg acttttgaac
    28741 aagctcagct ttggctagat gcaggtattt cacaagctat ttatcaccaa tctcgtgatg
    28801 ctcttcttgc tggtgaaact tggggtgaaa aagaccttaa taaggttaaa aaactcattg
    28861 acatgggctt ccgtgtatct gtaacaggtg gtctagatgt agatactctc aaactctttg
    28921 aaggtgttga tgtctttacc tttatcgcag gtcgtggaat tacagaggct gcggatccag
    28981 caggagcagc gcgtgccttc aaggatgaaa tcaaacgaat ttgggggtaa atcatggtac
    29041 gtccaattgg aatttatgaa aaggcaaccc caacacactg tacttggcta gaacgtttaa
    29101 attttgccaa ggagttaggc tttgattttg tcgagatgtc tattgacgaa cgtgacgagc
    29161 gtttagcaag acttgactgg agtaaggaag aacgcttgga agttgtcaaa gcaatctatg
    29221 aaactggtgt tcgtattcct tctatctgtt tttcaggcca tcgtcgctac ccattgggtt
    29281 caaaagatcc agttctagag gaaaaatctc tagaactcat gaaaaaatgt atcgaattag
    29341 ctcaagactt gggagttcgt acgattcaat tagctggtta cgatgtttac tatgaggaaa
    29401 agtcacccca gacacgccaa cgttttatca aaaatttgag aaaagcctgt gactgggctg
    29461 aagaagctca ggtggtactt gctattgaaa ttatggatga tcctttcatc aatagcatcg
    29521 aaaaatattt ggctatagaa aaagagattg actctccctt cctctttgta tatccagata
    29581 ttggtaatgt gtctgcatgg cataatgata tctatagtga gttttatctt ggtcatcatg
    29641 ccatcgcagc tctccatctc aaggatactt atgcagtgac agaaagttca aagggccagt
    29701 tccgagatgt accttttggg caaggttgtg tcaaatggga agaagctttc gatattttaa
    29761 aggaaaccaa ttataatgga cctttcctaa tcgaaatgtg gtctgaaaat tgtgaaacag
    29821 tagaagaaac acgcgcagcc attcaagagg cgcaagcttt tctctatcca ctcattaaga
    29881 aagcaggttt gatgtaagat gaatcaagta atcaatgcta tgcgtaaacg agtttgtgat
    29941 gccaatcaat cattgccaaa acatggactt gtcaaattta cctgggggaa tgtatctgaa
    30001 gtcaatcgcg aactcggtgt cattgttatc aaaccatcag gcgtggatta tgacgaattg
    30061 acacctgaaa acatggtagt gactgatcta gatggtaaga tcctagaagg ggatttaaga
    30121 ccatcttccg acctcccaac tcatgtgcaa ttatataagg cttggtcaga aattggtagt
    30181 gtggttcaca cccattcgac agaagctgtt ggttgggctc aggcaggtcg tgatattcct
    30241 ttctacggaa caacccatgc agattatttc tacggttcaa tcccttgcgc ccgtagtttg
    30301 accaaggacg aagtagaagt ggcctatgaa aaagatactg gcctggttat cgtagaagaa
    30361 tttgaacatc gcggacttaa cccggttgaa gtaccaggaa ttgttgtacg caatcacggt
    30421 ccattcacct ggggcaaaaa tccagagaat gctgtttatc actctgtcgt actagaggaa
    30481 gtatcaaaga tgaatcgctt tacagaacaa atcaatccaa gagttggacc tgctccccag
    30541 tacatactag aaaaacacta ccaacgtaaa catggaccaa atgcttatta tggtcaaaag
    30601 taagaacgat gaaggaggag aaaaagataa atttagctcc tctttttaca tttgattttt
    30661 attgagagta aagttggagt tgaagtaatt ttgaaagatt ttttagaaat agcgcttgat
    30721 atatatggta aaataaaaag aattgctgtg atatcaatag atttggggga ttttttaata
    30781 tggtactgga taaggcaagt tgtgatttgc ttcaatattt gatggatcaa gaaacgtcca
    30841 aaacgattat ggcgatttcg aaagatttga aagagtcaag aaggaaaatt tattatcaca
    30901 ttgacaaaat caatgctgct ctggttgacg aggcgcttca catcattagt attccacgaa
    30961 ttggtattca cttaacggaa gagcagagag atgcttgttg taaactatta tcggaagtag
    31021 attcgtacga ttatatcatg agtgcgcatg aacgtatgat gataatgtta ctatggatag
    31081 gtatttctaa agaacgtatt acgattgaaa aattgataga gttaacagag gtatctagga
    31141 atactgttct caatgatttg aatagtattc gttatcaact aactttggaa caatatcagg
    31201 tgatcttgca agtgagcaag tcacagggat accaccttca tgcccaccct cttaataaaa
    31261 ttcagtatct tcaatcgctt ctatatcata tttttatgga agaaaatgcc acttttgtat
    31321 ctattttaga agataagatg aaagagaggt tagatgatga gtgtttgctt tctgttgaaa
    31381 tgaaccaatt ttttaaggaa caggttcctt tagttgaaca agatttaggg aagaaaataa
    31441 accatcatga aataactttt atgttgcagg ttctacctta tttgctgtta agctgtcata
    31501 atgttgaaca gtatcaagaa agacatcagg atatagagaa agaattttct ttgataagaa
    31561 aaagaataga gtatcaggtg tctaagaaat taggagaacg gttgtttcaa aagtttgaaa
    31621 tttctttgtc aggacttgaa gtttctcttg tagctgttct cctcctctcc tatcgtaagg
    31681 atttggatat tcatgcagaa agtgatgatt ttcggcaatt aaaacttgct ttagaagaat
    31741 ttatctggta ttttgaatca caaatccgaa tggagattga gaacaaggat gatttgttac
    31801 gaaatttgat gatccactgt aaagccttgt tatttagaaa gacttacggt attttttcta
    31861 aaaatcctct aacaaaacaa attcgatcca agtatggaga attattttta gtcactagaa
    31921 aatctgcgga aattttagaa ggagcatggt ttattcggct aacagacgat gatattgcct
    31981 atttgacgat tcatattgga ggatttttaa agtatacacc atcgtctcaa aaaaatatga
    32041 aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc gagacttttg ctgaaacaat
    32101 gcaaacttta ttttccaaat gagcaaattg acactgtatt tacaacagaa caatttaaga
    32161 gtgtggaaga tattgcacaa gttgatgtag tgattactac taatgatgat ttggatagca
    32221 gatttccgat tttaagggtt aatcctatcc ttgaagcaga agatattttg aaaatgctag
    32281 actatcttaa acacaatata ttttgaaaat gctagactat cttaaacaca atatatttcg
    32341 taataagagc aaaagtttca gtgaaaatct ttctagtctt atttcgtctt atattgtaga
    32401 cagcaagttg gctagtaagt tccaagaaga ggttcaaaca cttataaatc aagaaatagt
    32461 agttcaagct tttttggaag atatttgaag gacagtccaa tgatgaacac aaacctgtgt
    32521 ttttcttggt cttttttagt gttttgaagg gtggtatact aatctcaaag ataacaatta
    32581 tatccaaagg aggcaacata tgccaaacgt caaagaaatt acaagagagt catggatttt
    32641 agccactttc ccagagtggg gaacatggtt gaacgaagaa atcgaagaag aagtcgtacc
    32701 tgaaggcaat tttgccatgt ggtggctagg caactgtggt acttggatta agacaccagc
    32761 tggtgctaac gttgtcatgg acctttggtc aaaccgtgga aaatcaacca aaaaagtgaa
    32821 agatatggtt cgtgggcacc aaatggcaaa tatggcaggt gttcgtaagt tgcaaccaaa
    32881 cttgcgtgtt cagccaatgg ttatcgatcc atttgctatc aacgaactag actattactt
    32941 agtttcacac ttccacagtg atcatatcga cccatacaca gctgcagcaa ttctcaacaa
    33001 ttctaagtta gagcatgtta agtttatcgg tccttaccac tgtggacgaa tctgggaagg
    33061 atggggtgtt ccaaaagaac gtatcatcgt tgttaaacca ggtgacacta tcgaattaaa
    33121 agatatgaag attcatgcag tagaatcatt tgaccgtact tgcttggtaa ctctcccagt
    33181 gaacggtgct gatgagacag gcggtgaact tgctggcttg gctgttacag atgaagaaat
    33241 ggctcaaaag gctgttaact atatctttga aacaccaggt ggaaccatct atcatggtgc
    33301 agattctcac ttctcaaact attttgcaaa acatggtaaa gactttaaaa ttgatgttgc
    33361 tttgaataac tatggtgaaa atccggtagg tatccaagac aaaatgacat ctatcgatct
    33421 tcttcgtatg gcagaaaatc tgcgtaccaa agtcattatc ccagttcact atgatatctg
    33481 gtctaacttc atggcttcta ctaatgagat tctagaactt tggaaaatgc gaaaagatcg
    33541 cttgcaatac gatttccatc catttatctg ggaagttggc ggtaagtaca cttatcctca
    33601 agatcaacac ttagtagaat accatcatcc acgtggtttt gatgattgtt ttgaacaaga
    33661 ctctaacatt caatttaaag ctttgctata atataaaaat attctttgga ggttatccgt
    33721 atgttgcatg atacggataa ttttcatata atagtaaaat agaatgtgtg attcaataat
    33781 cacctcaaat agaaaggaaa ttctatgtca aatctatctg ttaatgcaat tcgttttcta
    33841 ggtattgacg ccattaataa agccaactca ggtcatccag gtgtggttat gggagcggct
    33901 ccgatggctt acagcctctt tacaaaacaa cttcatatca atccagctca accaaattgg
    33961 attaaccgcg accgctttat tctttcagca ggtcatggtt caatgctcct ttatgctctt
    34021 cttcaccttt ctggttttga agatgtcagc atggatgaga ttaagagttt ccgtcaatgg
    34081 ggttcaaaaa caccaggtca cccagaattt ggtcatacgg cagggattga tgctacgaca
    34141 ggtcctctag ggcaagggat ttcaactgct actggttttg cccaagcaga acgtttcttg
    34201 gcagccaaat ataaccgtga aggctacaat atctttgacc actatactta cgttatctgt
    34261 ggagacggag acttgatgga aggtgtctca agcgaggcag cttcatacgc aggtttgcaa
    34321 aaacttgata agttggttgt tctttatgat tcaaatgata tcaacttgga tggtgagaca
    34381 aaggattcct ttacagaaag tgttcgtgac cgttacaatg cctacggttg gcatactgcc
    34441 ttggttgaaa atggaacaga cttggaagcc atccatgctg ctatcgaaac agcaaaagct
    34501 tcaggcaagc catctttaat tgaagtgaag acggttattg gatatggttc tccaaacaaa
    34561 caaggaacta atgctgtaca cggcgcccct cttggagcag atgaaactgc atcaactcgc
    34621 caagccctcg gttgggacta cgaaccattt gaaattccag aacaagtata tgctgatttc
    34681 aaagaacatg ttgcagaccg tggcgcatca gcttatcaag cttggactaa attagttgca
    34741 gattataaag aagctcatcc agaactggct gcagaagtag aagccatcat cgacggacgt
    34801 gatccagtcg aagtgactcc agcagacttc ccagctttag aaaatggttt ttctcaagca
    34861 actcgtaatt caagtcaaga tgccttgaat gttgtggcag ctaaattacc aaccttccta
    34921 ggtggatcag ctgaccttgc tcactcaaac atgacttata tcaagacgga tggacttcaa
    34981 gacgatacca atcgcttgaa tcgtaacatt cagtttggtg ttcgtgaatt tgcaatggga
    35041 acaatcttga acgggatggc ccttcatggt ggacttcgtg tatacggtgg aactttcttc
    35101 gtcttctctg actatgtgaa ggcagctgtc cgcttgtcag ccttacaagg acttcctgtg
    35161 acttatgtct ttacccatga ttcaatcgca gttggggaag atggtccgac tcacgaacca
    35221 gttgagcatt tagcaggtct tcgtgctatg ccaaatctaa atgttttccg tccagcagat
    35281 gcgcgtgaaa cgcaagcagc ttggtacctt gcagtgacaa gtgagaaaac accaactgcc
    35341 cttgtcttga cacgtcaaaa cttgactgtt gaagatggaa cagacttcga caaggttgct
    35401 aaaggtgctt atgttgtata tgaaaatgca gccgactttg ataccatctt gattgcgaca
    35461 ggttcagagg ttaatcttgc tgtctcagct gctaaagaat tggctagtca aggcgaaaaa
    35521 atccgcgtaa tcagcatgcc atctacagat gtctttgata aacaaaatgc agcttacaag
    35581 gaagagattc ttccaaatgc agtccgccgt cgtgttgcag tcgaaatggg tgcaagtcaa
    35641 aactggtaca aatatgttgg tctcgatggt gccgttctag gtattgatac cttcggagcc
    35701 tctgccccag caccaaaagt attggcagaa tatggcttta ctgtcgaaaa tcttgtaaaa
    35761 gttgttcgaa atttgaaata atcctaaaaa tcagggcgta agctctggtt tttcttacca
    35821 gaaaagtaag gtacaatctt gtaaaagtag ctgaaatttg atatagtagt cctatgtaaa
    35881 agacaaagga gaaaacaatg aatccaaata ttactttttt aatcatgctt gtaggtatga
    35941 tggccttgat gttctttatg caacgttctc aaaagaaaca agctcaaaag cgtatggaaa
    36001 gcttaaataa actacaaaaa ggctatgaag tgattacaat cggtggactt tatggaacag
    36061 tcgatgaagt agatacggag aagggaacaa tagttcttga cgtagatgga gtttacttga
    36121 cttttgaact agctgctatc aagacagtat taccgctgaa agaaacagct tcactagaag
    36181 gcgcaattga aaaataagac gggatcacga actcccgttt tctataaaag aaaggaaatg
    36241 ggatgaaaaa attagtcttt gtctgtctgg gaaatatttg ccgtagccct atggccgagt
    36301 ttgttatgaa atcaatgaca gataactacg aaatccaaag tcgagcaact tcctcttggg
    36361 aacatggcaa tccgattcat aaggggactc agggaatttt tcaagagtat gagattcctt
    36421 atgacaagaa caagacatcg cttcagatta gtaaggaaga ttttgaagcc tttgattata
    36481 ttatcggaat ggacgcttca aatatttctg acttacgtca gatgtgtcca gtagactgtc
    36541 aagataagat ttactcattt tcatctgaaa gtgttccaga cccttggtat acaggagatt
    36601 ttgaagaaac ctatcgacgt gttcaagagg gctgtcaagc ttggttagaa cgtttagaaa
    36661 aggagagtta aggtggaaaa tcttacgaat ttttacgaaa agtatcgtgt ctatctgact
    36721 cgtccacgtt tagagctttt ggcagtagtt accattgttt tctgtgctgt actcgtcttt
    36781 tttctaaata ttccaggaaa aggtgtctta aaactcgata atggaacgat tgtttatgat
    36841 ggcagtcttg tccgtggtaa aatgaatggc caaggtacca ttaccttcca aaatggagac
    36901 caatatacag gtggcttcaa caatggagcc ttcaacggaa aaggtacctt tcaatctaaa
    36961 gaaggctgga cctacgaagg tgattttgta aatggtcagg ctgaaggaaa agggaaacta
    37021 acaacagaac aagaagtcgt ttatgaagga acttttaaac aaggcgtttt tcaacaaaaa
    37081 taaagcctcc ttatcaaagg aggtattatt agaatcacaa ggtaagcgct tacctgtaaa
    37141 tccctttctt tccaaatccc tcttccaagc aagtttgtga aataaaaaat atttgaaata
    37201 aatttcacaa acttcaaaga taaaacttga taagaaaaga aaatgagaaa agtttcacaa
    37261 gagtttaaaa attctttgtg aaatgtttat gaaactgttt acaaagttga aataatgcgt
    37321 tataataaac ttgtgaaaaa attaacaaag gatatcgttc cttgaaagct atggaggaaa
    37381 atatggctga taaaaaaact gtgacaccag aggaaaagaa actcgttgct gaaaaacacg
    37441 tagatgagtt ggttcaaaaa gctctagttg cccttgaaga aatgcgtaaa ttggatcaag
    37501 aacaagttga ctacatcgtt gccaaagcat cagtagcagc tttggatgcc cacggagaat
    37561 tggctttaca tgcctttgaa gaaacaggac gtggtgtatt tgaagacaaa gcaactaaga
    37621 acttgtttgc ttgtgaacac gtagtaaaca atatgcgcca cactaagaca gttggcgtta
    37681 tcgaagaaga cgatgtaaca ggattgactc ttattgctga accagttggt gttgtttgtg
    37741 gtattactcc aacaacaaac ccaacatcaa cagcaatctt caaatcattg atttcattga
    37801 agacacgtaa cccaatcgtc tttgccttcc atccatcagc acaagaatca tctgctcatg
    37861 cagctcgtat cgtccgcgat gcagctatcg cagctggtgc tcctgaaaac tgtgtgcaat
    37921 ggattactca accatctatg gaagcaacaa gtgcccttat gaaccacgaa ggtgttgcga
    37981 caatccttgc aacaggtggt aatgccatgg ttaaggcggc ttattcatgt ggtaaaccag
    38041 ctcttggggt aggtgccgga aacgttccag cttatgttga aaaatcagca aacattcgtc
    38101 aagcagcaca cgatatcgtc atgtctaaat catttgataa cggtatggtc tgtgcatctg
    38161 aacaagcagt tatcattgat aaagaaattt acgatgaatt tgtagcagag ttcaaatctt
    38221 accacactta ctttgtaaac aaaaaagaaa aagctcttct tgaagagttc tgcttcggcg
    38281 tcaaagcaaa cagcaaaaac tgtgctggtg caaaattgaa cgctgacatc gttggtaaac
    38341 cagcaacttg gattgcagaa caagcaggat ttacagttcc agaaggaaca aacattcttg
    38401 ctgcagaatg taaagaagtt ggcgaaaatg agccattgac tcgtgaaaaa ttgtcaccag
    38461 ttattgcagt tttgaaatct gaaagccgtg aagatggtat tactaaggct cgtcaaatgg
    38521 ttgaatttaa cggtcttgga cactcagcag ctatccacac agctgacgaa gaattgacta
    38581 aagaatttgg taaagctgtt aaagctattc gtgttatctg taactcacct tctacttttg
    38641 gtggtatcgg ggacgtttac aatgccttct tgccatcatt gacacttgga tgtggttctt
    38701 acggacgcaa ctcagttggg gataacgtta gtgccattaa cctcttgaat atcaaaaaag
    38761 tcggaagacg gagaaataac atgcaatgga tgaaacttcc ttcaaaaaca tactttgaac
    38821 gtgattcaat tcaatacctt caaaaatgtc gtgacgttga acgtgtcatg atcgttactg
    38881 accatgccat ggtagagctt ggtttccttg atcgtatcat cgaacaactg gaccttcgtc
    38941 gcaataaggt tgtttaccaa atctttgcgg atgtagaacc ggatccagat atcacaactg
    39001 taaaccgtgg tactgagatt atgcgtgcct tcaaaccaga taccatcatc gcactcggtg
    39061 gtgggtctcc aatggatgct gccaaagtaa tgtggctctt ctacgagcaa ccagaagtgg
    39121 acttccgtga ccttgtccaa aaattcatgg atatccgtaa acgtgccttc aagttcccat
    39181 tgcttggtaa gaagactaaa ttcatcgcga ttccaactac atctggtaca ggatctgaag
    39241 taacaccatt tgccgttatc tctgataaag caaacaaccg taaataccca atcgctgact
    39301 actcattgac accaactgtg gcaatcgtag accctgcttt ggtattgaca gttccaggat
    39361 ttgttgctgc tgatactggt atggacgtat tgactcacgc gacagaagca tacgtatcac
    39421 aaatggctag tgactacact gatggtttag cacttcaagc cattaaattg gtctttgaaa
    39481 atctcgaaag ctcagttaag aatgcagact tccactcacg tgagaaaatg cataacgctt
    39541 caacaatcgc tggtatggcc tttgccaatg ccttcctagg tatttctcac tcaatggccc
    39601 ataagattgg tgcgcaattc cacacaatcc acggtcgtac aaatgctatc ttgcttccat
    39661 acgttatccg ttacaacggt acacgtccag ctaagacagc aacatggcct aagtacaact
    39721 actaccgtgc agatgaaaaa taccaagata tcgcacgcat gcttggactt ccagcttcta
    39781 ctccagaaga aggggttgaa tcttacgcaa aagctgtcta cgaactcggt gaacgtattg
    39841 ggatccaaat gaattttaga gaccaaggaa ttgacgaaaa agaatggaaa gaacattctc
    39901 gtgaattatc cttcctggct tatgaagacc aatgttcacc agctaaccca cgtcttccaa
    39961 tggtagacca tatgcaagaa atcatcgaag atgcatacta tggctacaaa gaaagaccag
    40021 gacgccgtaa ataattgttt atcagtctag aagcaagaca aaaactcaat ttgagggaaa
    40081 gatccagtaa tttttctatg ataaaaggca tcctatcaag gtttttgaat acctgatagg
    40141 atgccttttt atgatgttga ggcctttttg ccctttttga aaaactagaa tagaaacaaa
    40201 atatatagca gattgaaact agaatagtac atatctgctt ctaaaacatt gttagaattc
    40261 ga
//
LOCUS       DAVLWA010000009        40152 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLWA010000009 DAVLWA010000000
VERSION     DAVLWA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40152
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.009"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..213
                     /locus_tag="VYN80_000454"
     CDS             <1..213
                     /locus_tag="VYN80_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEV1540414.1"
                     /translation="DVHCDAVMVKDVNEDLMKGYDIFTPIAATDLGFEPGIPVIEAGP
                     ILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            261..1736
                     /locus_tag="VYN80_000455"
     CDS             261..1736
                     /locus_tag="VYN80_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1540415.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2041..8742
                     /locus_tag="VYN80_000456"
     CDS             2041..8742
                     /locus_tag="VYN80_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1540416.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8990..9846)
                     /locus_tag="VYN80_000457"
                     /pseudo
     CDS             complement(8990..9846)
                     /locus_tag="VYN80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10057..10416)
                     /locus_tag="VYN80_000458"
     CDS             complement(10057..10416)
                     /locus_tag="VYN80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1540417.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            10508..11464
                     /locus_tag="VYN80_000459"
                     /pseudo
     CDS             10508..11464
                     /locus_tag="VYN80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11448..12005
                     /locus_tag="VYN80_000460"
     CDS             11448..12005
                     /locus_tag="VYN80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1540418.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11998..12252
                     /locus_tag="VYN80_000461"
     CDS             11998..12252
                     /locus_tag="VYN80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540419.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12264..14318
                     /locus_tag="VYN80_000462"
     CDS             12264..14318
                     /locus_tag="VYN80_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1540420.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14497..15357
                     /locus_tag="VYN80_000463"
     CDS             14497..15357
                     /locus_tag="VYN80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1540421.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15568..16278
                     /gene="ccdA2"
                     /locus_tag="VYN80_000464"
     CDS             15568..16278
                     /gene="ccdA2"
                     /locus_tag="VYN80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1540422.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16298..16864
                     /locus_tag="VYN80_000465"
     CDS             16298..16864
                     /locus_tag="VYN80_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1540423.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16875..17987
                     /gene="msrB"
                     /locus_tag="VYN80_000466"
     CDS             16875..17987
                     /gene="msrB"
                     /locus_tag="VYN80_000466"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1540424.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEIMLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18255..18992
                     /locus_tag="VYN80_000467"
     CDS             18255..18992
                     /locus_tag="VYN80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540425.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            18989..20680
                     /locus_tag="VYN80_000468"
     CDS             18989..20680
                     /locus_tag="VYN80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1540426.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20796..21359
                     /locus_tag="VYN80_000469"
     CDS             20796..21359
                     /locus_tag="VYN80_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1540427.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            21381..27050
                     /locus_tag="VYN80_000470"
     CDS             21381..27050
                     /locus_tag="VYN80_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1540428.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            27111..28832
                     /gene="pabB"
                     /locus_tag="VYN80_000471"
     CDS             27111..28832
                     /gene="pabB"
                     /locus_tag="VYN80_000471"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1540429.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            28952..30478
                     /locus_tag="VYN80_000472"
     CDS             28952..30478
                     /locus_tag="VYN80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1540430.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            30565..31484
                     /locus_tag="VYN80_000473"
                     /pseudo
     CDS             30565..31484
                     /locus_tag="VYN80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            31619..32578
                     /locus_tag="VYN80_000474"
     CDS             31619..32578
                     /locus_tag="VYN80_000474"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1540431.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEEDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            32675..33514
                     /locus_tag="VYN80_000475"
     CDS             32675..33514
                     /locus_tag="VYN80_000475"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1540432.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(33562..33732)
                     /locus_tag="VYN80_000476"
     CDS             complement(33562..33732)
                     /locus_tag="VYN80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1540433.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33853..34737
                     /gene="miaA"
                     /locus_tag="VYN80_000477"
     CDS             33853..34737
                     /gene="miaA"
                     /locus_tag="VYN80_000477"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1540434.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            34730..35968
                     /gene="hflX"
                     /locus_tag="VYN80_000478"
     CDS             34730..35968
                     /gene="hflX"
                     /locus_tag="VYN80_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1540435.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35961..36584
                     /locus_tag="VYN80_000479"
     CDS             35961..36584
                     /locus_tag="VYN80_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1540436.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            36599..37528
                     /locus_tag="VYN80_000480"
     CDS             36599..37528
                     /locus_tag="VYN80_000480"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1540437.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            37549..38304
                     /locus_tag="VYN80_000481"
     CDS             37549..38304
                     /locus_tag="VYN80_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1540438.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(38358..39266)
                     /locus_tag="VYN80_000482"
     CDS             complement(38358..39266)
                     /locus_tag="VYN80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1540439.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            39330..39599
                     /locus_tag="VYN80_000483"
     CDS             39330..39599
                     /locus_tag="VYN80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1540440.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            39599..39979
                     /locus_tag="VYN80_000484"
     CDS             39599..39979
                     /locus_tag="VYN80_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1540441.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT        12451 a         7344 c         8887 g        11470 t
ORIGIN      
        1 gatgttcact gtgatgcagt catggtgaaa gatgtaaacg aagaccttat gaaaggttac
       61 gatatcttta caccaatcgc tgcaacagac cttggttttg aaccaggtat cccagttatc
      121 gaagctgggc caatcttatt ccgtatccca gcaatgagcg ctccagtatt tgacaatatt
      181 gaagcagcta ttaaagaaca tggattaagc taattctaat ttgttaacat tcatataaca
      241 taaaaaaggg aggaactgtt atggatgcaa tctttgacct aatcggaaag gttttcaatc
      301 ccatcttaga aatgggtgga cctgtcatca tgttgatcat tttgacagta ttggctttac
      361 tttttggagt gaaattctcc aaagcgcttg aaggtggtat caaacttgcc atcgctctta
      421 caggtatcgg tgctatcatc ggtatgctaa atggtgcttt ctcagcatca cttgcaaaat
      481 tcgttgaaaa cactggtatc caattgagta ttacagacgt tggttgggca ccactcgcaa
      541 caatcacttg gggttctgct tggacactat acttcttgct catcatgttg attgtcaacg
      601 tagtgatgct tgctatgaag aaaaccgata cacttgatgt cgatatcttt gatatctggc
      661 acttgtctat cacaggtctc ttgattaaat ggtatgctga taacaatggt gtgagtcaag
      721 gggtttcact ctttattgct acagcagcta tcgtccttgt cggtgtgttg aaaattatca
      781 actctgactt gatgaaacct acatttgatg accttcttaa cgccccaagt tcatcaccaa
      841 tgacatcaac tcacatgaac tacatgatga acccagttat catggttttg gataagattt
      901 ttgaaaaatt cttcccaggc cttgataaat atgactttga tgctgctaaa ttgaacaaga
      961 aaatcggttt ctggggatct aaattcttca tcggtttcat ccttggtatc gttatcggta
     1021 ttatgggaac tccacatcca attgcaggtg ttgcagatgc agataaatgg cgtcttgtta
     1081 tcaaaggatg gttgtctctt ggtttgactg ccggtgtatc tttggaactc ttctcactta
     1141 tcggttcatg gttcatcgca gccgtagaac cactatcaca aggtattaca aacgttgcta
     1201 ctaaacgtct tcgaggacgt aaattcaata tcggtcttga ctggccattc atcgctggtc
     1261 gtgctgaaat ctgggcttgt gccaacgtac ttgcaccaat catgttgatt gaagcagtgc
     1321 ttctttcaaa agttggaaat ggtatcttgc cacttgcagg tatcatcgct atgggtgtta
     1381 ctccagctct cttggttgta actcgtggta aattgctccg tatgattatc ttcggaacac
     1441 tcttgttgcc actcttcctt ctttcaggta cacttattgc accatttgca acagaacttg
     1501 ctaaaggtgt aggtgccttc ccagaaggtg tgagccaaac tcaattgatt actcactcta
     1561 ctcttgaagg accaatcgaa aaacttcttg gttggacaat tggtaacact acaactggtg
     1621 atatcaaagc aatccttggt gcagtagtct tccttgtatt ctatatcggt atctttgctt
     1681 ggtacagaaa acaaatgatc aaacgtaacg aagagtacgc agcaaaagca aaataatgcg
     1741 ctcctataaa atataaactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt
     1801 tgctaaaaac acagttttga ggttgtagat aagactgacg aagtcagctc aaaacatggt
     1861 tttgaggttg tagatgaaac tgacgaagtc agtaatcata tctacggcaa ggcgacgctg
     1921 acgaagtttg aattggattt tcgcagagtg taaattgttg aaactaggtt gtcataccat
     1981 gtataccact tgggctttgg tagtagctaa ctgcactaaa tataatataa ggagggtttt
     2041 atggggaaag gccattggaa tcggaaaaga gtttatagca ttcgtaagtt tgctgtggga
     2101 gcttgctcag taatgattgg gacttgtgca gttctattag gaggaaatat agctggagaa
     2161 tctgtagttt atgcggatga aacacttatt actcatactg ctgagaaacc taaagaggaa
     2221 aaaatgatag tagaagaaaa ggctgataaa gctttggaaa ctaaaaatgt agttgaaagg
     2281 acagaacaaa gtgaacctag ttcaactgag gctattgcat ctgagaagaa agaagatgaa
     2341 gccgtaactc caaaagagga aaaagtgtct gctaaaccgg aagaaaaagc tccaaggata
     2401 gaatcacaag cttcaagtca agaaaaaccg ctcaaggaag atgctaaagc tgtaacaaat
     2461 gaagaaatga atcaaatgat tgaagacagg aaagtggatt ttaatcaaaa ttggcacttt
     2521 aaactcaatg caaattctaa ggaagccatt aaacctgatg cagacgtatc tacgtggaaa
     2581 aaattagatt taccgtatga ctggagtatc tttaacaatt tcgatcatga atctcctgca
     2641 caaaatgaag gtggacaact caacggtggg gaagcttggt atcgcaagac tttcaaacta
     2701 gatgaaaaag acctcaagaa aaatgttcgc cttacttttg atggcgtcta catggattct
     2761 caagtttatg tcaatggtca gttagtgggg cattatccaa atggttataa ccagttctca
     2821 tatgatatca ccaaatacct tcacaaagat ggtcgtgaga atgtgattgc tgtccatgca
     2881 gtcaacaaac agccaagtag ccgttggtat tcaggaagtg gtatctatcg tgatgtgact
     2941 ttacaagtga cagataaggt gcatgttgag aaaaatggga caactatttt aacaccaaaa
     3001 cttgaagaac aacaacatgg caaggttgaa actcatgtga ccagcaaaat cgtcaatacg
     3061 gacgacaaag accatgaact tgtagccgaa tatcaaatcg ttgaacgagg tggtcatgct
     3121 gtaacaggct tagttcgtac agcgagtcgt accttaaaag cacatgaatc aacaagccta
     3181 gatgcgattt tagaagttga aagaccaaaa ctctggaccg ttttaaatga caaacctgcc
     3241 ttgtacgaat tgattacgcg tgtttaccgt gacggtcaat tggttgatgc taagaaggat
     3301 ttgtttggtt accgttacta tcactggact ccaaatgaag gtttctcttt gaatggtgaa
     3361 cgtattaaat tccatggagt atccttgcac cacgaccatg gggcgcttgg agcagaagaa
     3421 aactataaag cagaatatcg ccgtctcaaa caaatgaagg agatgggagt taactctatc
     3481 cgtacaaccc acaaccctgc tagtgagcaa accttgcaaa tcgcagcaga actaggttta
     3541 ctcgttcagg aagaggcctt tgatacgtgg tatggtggca agaaacctta tgactatgga
     3601 cgtttctttg aaaaagatgc cactcaccca gaagctcgaa aaggtgaaaa atggtctgat
     3661 tttgacctac gtaccatggt cgaaagaggc aaaaacaacc ctgctatctt catgtggtca
     3721 attggtaatg aaataggtga agctaatggt gatgcccact ctttagcaac tgttaaacgt
     3781 ttggtcaagg ttatcaagga tgttgataag actcgctatg ttaccatggg agcagataaa
     3841 ttccgtttcg gcaatggtag cggagggcat gagaaaattg ctgatgaact cgatgctgtt
     3901 ggatttaact attctgaaga taattacaaa gcccttagag ctaagcatcc aaaatggttg
     3961 atttacggtt cagaaacatc atcagcaacc cgtacacgag gaagttacta tcgccctgaa
     4021 cgtgaattga aacatagcaa tggacctgag cgtaattatg aacagtcaga ttatggaaat
     4081 gatcgtgtgg gttgggggaa aacagcaacc gcttcatgga cttttgaccg tgacaacgct
     4141 ggctatgctg gacagtttat ctggacaggt acggactata ttggtgaacc tacaccatgg
     4201 cacaaccaaa atcaaactcc tgttaagagc tcttactttg gtatcgtaga tacagccggc
     4261 attccaaaac atgacttcta tctctaccaa agccaatggg tttctgttaa gaagaaaccg
     4321 atggtacacc ttcttcctca ctggaactgg gaaaacaaag aattagcatc caaagtagct
     4381 gactcagaag gtaagattcc agttcgtgct tattcgaatg cttctagtgt agaattgttc
     4441 ttgaatggaa aatctcttgg tcttaagact ttcaataaaa aacaaaccag cgatgggcgg
     4501 acttaccaag aaggtgcaaa tgctaatgaa ctttatcttg aatggaaagt tgcctatcaa
     4561 ccaggtacct tggaagcaat tgctcgtgat gaatctggca aggaaattgc tcgagataag
     4621 attacgactg ctggtaagcc agcggcagtt cgtcttatta aggaagacca tgcgattgca
     4681 gcagatggaa aagacttgac ttacatctac tatgaaattg ttgacagcca ggggaatgtg
     4741 gttccaactg ctaataatct ggttcgcttc caattgcatg gccaaggtca actggtcggt
     4801 gtagataacg gagaacaagc cagccgtgaa cgctataagg cgcaagcaga tggttcttgg
     4861 attcgtaaag catttaatgg taaaggtgtt gccattgtca aatcaactga acaagcaggg
     4921 aaattcaccc ttactgctca ctctgatctc ttgaaatcta gtcaagtcac tgtctttact
     4981 ggtaagaaag aaggacaaga aaagactgtt ttggggacag aggtgccaaa agtacagacc
     5041 attattggag aggcacctga aatgcctacc actgttccgt ttctatacag tgatggtagt
     5101 cgtgcagaac gtcctgtaac ctggtcttca gtagatgtga gcaagcctgg tattgtaacg
     5161 gtgaaaggta tggctgacgg acgagaagta gaagctcgtg tagaagtgat tgctcttaaa
     5221 tcagagctac cagttgtgaa acgtattgct ccaaatactg acttgaattc tgtagacaaa
     5281 tctgtttcct atgttttgac tgatggaagt gtacaagagt acgaagtaga tagctgggag
     5341 attacggaag tagataaagc caaactttca gtagctggat cacgtattca aatgactggt
     5401 cagttagctg gagaaactat tcatgcaacc cttgtggtag aagaaggaaa tgctgcagca
     5461 cctgtagtgc caactgttac tgttggaggt gaagcagtaa caggtcttac tagtcgacaa
     5521 ccaatgcaat atcgtactct atcttatggt gcccaattgc cagaagtcac agcaagtgct
     5581 gaaaatgctg atgtgacagt tcttcaagca agcgcagcaa acggcatgcg tgcaagcata
     5641 tttgttcagc ctaaagatgg tggccctctt caaacctatg caattcaatt cctagaagaa
     5701 gcaccaaaaa ttgctcactt gagcttgcaa gtggaaaaag ctgacagtct caaagaagac
     5761 caaactgtca aattgtcggt tcgagctcac tatcaagatg gaacgcaagc tgtattacca
     5821 gctgataaag taaccttctc tacaagtggt gaaggggaag tcgcaattcg taaaggaatg
     5881 cttgagttgc ataagccagg agcagtcact ctcaaagcgg aatatgaggg agctaaaggc
     5941 caagttgaac tcactatcca agccaatact gagaagaaga ttgcgcaatc tatccgtcct
     6001 gtaaatgtag tcacagattt gcatcaaaaa cctactcttc caacaacagt aacggttgag
     6061 tatgacaaag gtttccctaa agctcacaaa gtcacttggc aagctattcc gaaagaaaaa
     6121 ctagactcct atcaaacatt tgaagtacta ggtaaagttg aaggaattga ccttgaagcg
     6181 cgtgcaaaag tctctgtaga aggtatcgtt tcagttgaag aagtcagtgt gacaactcca
     6241 atcgcagaag caccacaatt accagaaagt gttcggacat atgattcaaa tggtcacgtt
     6301 tcatcagcta aggttgcatg ggatgcgatt cgtccagagc aatacgctaa ggaaggtgtc
     6361 tttacagtta atggtcgctt agaaggtact caattaacaa ctaaacttca tgttcgcgta
     6421 tctgctcaaa ctgagcaagg tgcaaacatt tctgaccaat ggaccggttc agaattgcca
     6481 cttgcctttg cttcagactc aaatccaagc gacccagttt caaatgttaa tgacaagctc
     6541 atttcctaca ataaccaacc agccaatcgt tggacaaact ggaatcgtac taatccagaa
     6601 gcttcagtcg gtgtcctatt cggagattca ggtattttga gcaaacgttc agttgataac
     6661 ttaagcgttg gcttccatga agaccatgga gttggtgcac cgaagtctta tgtgattgag
     6721 tattatgttg gtaagactgt cccaacagct cctaaaaacc ctagttttgt tggtaatgag
     6781 gaccatgtct ttaatgattc tgccaactgg aaaccagtta ctaatctaaa agcccctgct
     6841 caactcaagg ctggagaaat gaaccacttt agctttgata aagttgaaac ctatgctgtt
     6901 cgtattcgca tggtgagagc agacaacaaa ctaggaacgt ctatcacaga ggtacaaatc
     6961 tttgcgaaac aagttgcggc agccaagcaa ggacaaacaa gaatccaagt tgacggtaaa
     7021 gacttagcaa acttcaaccc tgatttgaca gactactacc ttgagtctgt agatggaaaa
     7081 gttacggcag tcacagcaag tgttagcaac aatggcctcg ctaccgtcgt tccaagcgtt
     7141 cgtgaaggtg agccggttcg tgtcatcgcg aaagctgaaa atggcgacat cttaggagaa
     7201 taccgtctgc acttcactaa ggataagaac ttactttctc ataaaccagt tgctgcggtt
     7261 aaacaagctc gcttgctaca agtaggtcaa gcacttgaat tgccgactaa ggttccagtt
     7321 tacttcacag gtaaagacgg ctacgaaaca aaaaacctga cagttgaatg ggaagaagtt
     7381 ccagcggaaa atctgataaa agcaggtcaa tttaccgttc gaggccatgt ccttggtagt
     7441 gatcttgttg ctgaggtcac tgtacgagtg acagacaaac taggcgaagc tctttcagat
     7501 aaccctaact atgatgaaaa cagtaaccag gcctttgctt cagcaaccaa tgatattgac
     7561 aaaaactctc atgaccgcgt tgactatctc aatgacggag atcattcaga aaatcgtcgt
     7621 tggacaaact ggtctccaac accatcttct aatccagaag tatcagcggg tgtgatcttc
     7681 cgtgaaaatg gtaagattgt agaacggact gttgctcaag ccaaacttca cttctttgca
     7741 gatagtggta cggatgcacc aactaaactc gttttagaac gctatgtcgg tccagagttt
     7801 gaagtgccaa cctactattc aaactaccaa gcctacgacg cagaccatcc attcaacaat
     7861 ccagaaaatt gggaagctgt gccttatcgt gcggataaag acatcgcagc tggtgatgaa
     7921 atcaacgtaa catttaaagc tgtcaaagcc aaagctatga gatggcgtat ggagcgtaaa
     7981 gcagataaga gcggtgttgc gatgattgag atgaccttcc ttgcaccaag tgaattgcct
     8041 caagaaagca cgcaatcgaa gattcttgta gatggaaaag aacttgctga tttcgctgaa
     8101 aatcgtcaag actatcaaat tacctataaa ggtcaacggc caaaagtctc agttgaagaa
     8161 aacaatcaag tagcttcaac tgtggtagat agtggagaag atagccttcc agtacttgtt
     8221 cgcctcgttt cagaaagtgg aaaacaagtc aaggaatacc gtatccagtt gactaaggaa
     8281 aaaccagttt ctgagaagac agttgctgct gtacaagaag atcttccaaa actcgaattt
     8341 gttgaaaaag atttggccta caagacagtt gagaaaaaag attcaacact gtatctaggt
     8401 gaaactcgtg tagaacaaga aggaaaaact ggtaaagaac gtatctttac agcgattaat
     8461 cctgatggaa gtaaggaaga aaaactccgt gaagtggtag aagctccgac agaccgcatc
     8521 gtcttggttg gaaccaaacc agtagctcaa gaagctaaaa aaccacaagt gtcagaaaaa
     8581 gcagatacaa aaccaattga ttcaagtgaa gctagtcaaa ctaataaagc ccagttacca
     8641 aatacaggta gtgcggcaag ccaagcagca gtagcagcag gtttagctct tctaggtttg
     8701 agtgcaggat tagtagttac taaaggtaaa aaagaagact agaaattttg ataccttctt
     8761 tatcattaaa gaccaaagaa aaactcttgt cctaaataga agataaagag tctggaacaa
     8821 aatagttctc aaccacaaaa aagctagata tttccaattg cggaactcta gctttttaat
     8881 tttgagtcgt tctcttattg tatagtagat tgaaactaga gtagtacact tctacttcta
     8941 aaacattgtt agaaatcgat ttgactgtcc ttatcgattt gtcctgttct tatttcattt
     9001 cactatgttt taggaggtta ttggccataa gtaccaatcc catgtcaatt ctcacttgac
     9061 gcttgcctct cagattacat ctcttgtaac ccaaacaagc ctttatctgc ccaaagacag
     9121 gttctacatc aatcttgcgt tgagcgaaaa tctgtctatc ttggggagat aaaagcgcct
     9181 gacattcttt agctttcaag ttttgatagc gttcgttcat atacaatccc ttttgagggg
     9241 ctgattcagg ttcatcagcg tagtaaacct tgatttcctg ttgaaagtct gtctgtgttt
     9301 tctggtactt gatatggtga aaacgatagc accagccatc aggatgtatg tagctatcct
     9361 ccttgtcatt atagtgccaa ttcgctaagt ttttagctga ctgtttatac cctctctttt
     9421 gttccttatc aaacatggca tatttaatca gaaggggagt gatttcttct tgaaaataga
     9481 cttgtatctg ttcttgaagt ttggcggaga atttctctgt cgttttcttc cacacaaaac
     9541 tatacttgtt ggcattggat tcaatcttag ttccgtcaat aaacaaacaa tctaaggtca
     9601 ctaactcttc catttttcaa tttttcgacc agagaaaatc ccttgtgaat aggcaaatag
     9661 aagagtagat acaagcattt tagggtgata agacgggcga ccaaaggcat gataggaggt
     9721 gtagaagtga cgttcctcca aggtattcac cactttttca atagtaaaga cgagatggtc
     9781 ttgtggtaag aaggaactga tttctagtgg taaagttgtt tgatttgtgt tatagtgaat
     9841 atgcatggga tagcctttct aaatggttta ttaagcaatt ctattttacc aagactatcg
     9901 taaccatgca aaaaagcaac ggcaaatccg ttgccttttt tggatataag agactattgt
     9961 cccagactct tttctttgta taaaattgta tagtgttatt gtctatacta ttttgtagag
    10021 aaagcaaaaa tccctagaaa atgcttaggg attcgactat ttcattaaga acaattcaac
    10081 aatcatctct atatacatga taagggttaa taaaaatcct gtacgccaga agaatttgat
    10141 gaatttagga tagtaaaagc tgcgtttttt taaaaggaag aaaaagacga gaataatggc
    10201 taggaacgag agggctagcc ccagtctagg gaggaaatta tgggtaaagg ttttagctgt
    10261 aatcaagtaa tactcaaata ccaagactgg aaaagccaaa tccgcaaagt ttcgtcctag
    10321 tttttttaat ctaaaaagtt tggttaggat aatgcagacg actaaggtca gtatcaataa
    10381 taaaatagat gctaatttca ttaaaatcat acccatattg tatcataaaa aaaggctaat
    10441 ggaaacgaga aacagaggaa tttataggaa agtcaggctt ttgagattgt gggtgatttt
    10501 tgttataatg aaagttataa aatcttatga taccttgaat gattattatc gaaaactctt
    10561 tggagaaaag acttttaaag tccctattga tgcgggattt gactgtccca atcgtgatgg
    10621 aactgtggct catggaggct gtactttttg tacggtttcc ggttctggag atgccattgt
    10681 agccccagat ccgcctatcc gtgagcaatt ttataaggaa attgacttta ttcatcgtaa
    10741 gtggccagat gttcagaagt atctggttta ttttcaaaat tttaccaaca cccatgaaaa
    10801 ggtggaagtt attcgagagc gttatgaaca ggctatcaat gagccaggtg tggtaggaat
    10861 caatattgga acgcgcccag actgtttacc agacgaaacc atcgaatatt tggctgagtt
    10921 atcggagtgc atgcatgtga cggttgaatt gggcttgcag actacttatg aagcaacctc
    10981 tgacctgatt aaccgtgcgc attcttatga attgtaggtg gaaacggtca agcgtttgag
    11041 aaagtatcca aaaattgaga ttgtttccca tttgatcaat ggtttgcctg gtgaaaccca
    11101 tgagatgatg gttgaaaatg tccgccgttg tgtcacggat aacgatattc aagggattaa
    11161 actgcatttg ctccatctta tgacaaatac tcgtatgcaa cgagattacc atgagggacg
    11221 tttacaattg atgagtcagg acgaatatgt cagggtcatc tgtgaccaac tggaaattat
    11281 tcccaaacat atcgtcatcc atcgaattac aggagatgcg cctagggata tgctgattgg
    11341 tcctatgtgg agcctcaata aatgggaagt cctcaacagc attgagatgg agatgagacg
    11401 tcgtggaagt gttcaaggat gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac
    11461 ttgagatggc acatgatttt ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg
    11521 cgactatggg aaatggtcat gacacgcttt ttttagccaa gctagccaag caagtctatg
    11581 cctttgatat tcagaagcaa gccttggaaa agacccaaga gcgtttgcat caggctggct
    11641 tgacaaatgc ccagttaatc ttgcaaggcc atgagacact ggaccagttt gtgacaaaag
    11701 ctaaggcagg gatttttaat ttgggctatt tgccgtcagc tgataagtct gtcatcaccc
    11761 gaccgcagac aacgattgag gcattagaaa agctatgtgg cttacttgtc aaaggtggac
    11821 gaattgctat tatgatttac tatggtcatg aaggaggcga cctcgagaga gatgctgtct
    11881 tggattttgt gatccagttg aaccaacaag agtacacagc tgccatttac cgaactttaa
    11941 accaagtcaa caacccgccg tttttagtga tgattgaaaa attagagaga tacagacatg
    12001 gataaacaat acctacacga aaagctggat gccatgcgcc agaattttgt tgaatcaacc
    12061 taccacgaac gagcggtggg cgtgctagac caagcgcata tgagcaaaaa aatgcttaaa
    12121 atcaagaaaa aattagttgc tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa
    12181 gactgttcca agattgacca aaaaatcaaa gagcagaagg agatatttga atcctgttgt
    12241 aaaaaagatt aaggaggacg tgcgtggaat tactgattta cctcatccta tttttactgg
    12301 tcttgattgt ctcaagtaca accaataagc tcctaccctt tttgcctctc cctttggtgc
    12361 agattctttt gggaattgtg attggtctct ttttacccaa tactgacttt caccttaata
    12421 cggagttgtt tttggcactg gttatcggcc ccttgctttt ccgagaggct gaagaagcag
    12481 atgttacggc tattttaaaa cactggcgaa tcattgttta tctcatattt ccagtgattt
    12541 ttatctcgac cctgagtttg ggtggcttgg cccatcttct ttggttcagc cttcccttgg
    12601 cagcttgctt ggctgttggg gcagcccttg gtcctacgga cttggtggcc tttgcctctc
    12661 tttcggagcg ttttagcttt cctaagcgcg tgtccaatat tcttaagggc gaaggactct
    12721 tgaatgatgc ttctggtttg gtggcttttc aggtagcttt gacagcttgg acaactggag
    12781 ctttttctct ggggcaagct agcagttcac tcatcttttc aatcctaggc ggttttttaa
    12841 ttggattttt aacagctatg accaaccgct tcctccatac cttcttgcta agtgtgcgcg
    12901 caacggatat tgccagtgaa cttttattag agttgagttt gcctctagtg accttctttc
    12961 tggcagaaga agtccatgtt tcagggatta ttgccgtcgt agttgctgga attttaaagg
    13021 caagtcgctt caagaaaatc acgctcctcg aagcccaagt ggatacggtg accgagacgg
    13081 tctggcatac agtgaccttt atgctcaacg gttctgtctt tgtgatttta gggatggagt
    13141 tggaaatgat agcagaacct atcttgacca atccaatcta taatcctcta cttttattgc
    13201 tatctctcat cgcccttacc tttgtcctct ttgtcattcg ttttattatg atctatggct
    13261 attatgccta tagaacccga cgcctcaaga aaaagctaaa taagtatatg aaggacatgt
    13321 ttctcttgac cttttcaggt gttaagggaa cggtgtcgat tgctacgatt ctcttgatac
    13381 caagtaatct agaacaggag tatcctctct tgcttttcct tgttgcaggt gtgacgcttg
    13441 tcagcttttt aacaggtctc ttggtcttgc ctcatctttc tgatgaagag gaagaaagca
    13501 aggattatct catgcatatc gccattttga atgaagtaac gctagagttg gaaaaagagt
    13561 tggaagacac cagaaataaa cttcccctct atgcggctat tgacaattat catggacgta
    13621 ttgaaaatct cattttaagc caagaaaacc aggatgatca agaagactgg gctgctttga
    13681 aactcttgat tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa gaggggaaca
    13741 ttagcaatcg tgcttaccga gtttaccaac gttatctgaa aaatatagaa caaggaatca
    13801 atcgtaaact tgcctcaaga ctgacctatt attttcttgt ttccttgagg attttacgtt
    13861 ttctccttca tgaagttttt actcttggaa agaccttccg tagctggaag gacaaggagc
    13921 aaagccgtct ccgtgctctt gattatgacc aaattgcaga gctctatctt gccaatacag
    13981 agatgattat tgaaagtttg gaaaacctga agggagtcta cagacgctct ttgattagtt
    14041 ttatgcagga gtctcgtctt cgagaaacag ctattatcag cagtggtgcc tttgtcgaac
    14101 gggttatcaa tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc tattatctgg
    14161 agcgcaagtt gattttcgaa tacgaagaaa aacgattgat tacgactaag tatgccaaga
    14221 aattacgaca aaatgtaaat aacttagaga actattcctt gaaggaagct gccaataccc
    14281 taccgtatga tatggtggaa ttggtaagaa gaaattagtt aatactcttc gaaaatctct
    14341 tcaaaccacg tcagcgtcgc cttaccgtat gtatgtgact gacttcgtca gtcttatcta
    14401 caacctcaaa acagtgtttt gagcagcttg cggctagctt tctagtttgc tctttgattt
    14461 tcattgagta taagattata agtgaaggag tatgacatga aaaagtggtg gaaagagctg
    14521 atagacaaac ctttattaaa agcttttttg cattattatc aagcatcaga tagtgagttg
    14581 accagtgtcg cagtagccta ctattggttg atttcgattt tccccctgct tttggtggtg
    14641 gtcaatatcc tcccctattt tcagattcct gtgggagaat ttctgggctt tatgaaagac
    14701 gtcctgcccc caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt cttgacgcaa
    14761 ccttcaacag gtttattgag tttttctgtt ttatcggcgt tatggagttt ttcaaaatct
    14821 atgaatttct tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag tcgtggactt
    14881 atttcccatc agatgctaag tctcctagtc agttttggct tgcagctcct ctttgcgttt
    14941 gccttgtttt tgatcttatt tggtcagatg attttggctt tacttgctca ttattggaca
    15001 aaagatggca ttatctatca ggctttacaa gggttggcag gtcctctgat ttacgccctc
    15061 ctctttgcta tcttggtcat gctttattat tttcttccca atctatctaa caggaaaatt
    15121 agctataccc taccaggcag tgcctttgtt ctcttagtga ttttaggttt actaactctg
    15181 ttctctagtt atctcaatta ctatgtccat catttggtgg atgtccgaat tttaggttcc
    15241 gttcttcttg ttgttatgat gttttggttt atcttgattg ctaaaattgt tatcctaggc
    15301 gcggttatca atgccagtat gcagagtttg aaagatccgg tctttaaaaa agactaacta
    15361 tttatccatt ataaaagcgc atagtatcaa gactttatag aaccttgata ctatgcgctt
    15421 ttgtgctttg acagtttttt gtatagtttt tgtccaagat ttctaaatca tctgattaaa
    15481 ttcctatttt cccctatcat tgtccctgtt ttttcgaaaa tggaaggagt ataataaatc
    15541 tatcaataaa atgataggag gaactttatg ggtcatattt tcttttttct aagtgtcttt
    15601 ttggcaggga ttctatcctt cttttctcct tgtatcttac ctttgttacc ggtctataca
    15661 ggagtgttac tagatgataa ggatggtgct caggcttcta gcggcaaatt ttcaatctca
    15721 gttactagtt tattacgaac tctggccttt atagcaggaa tttcctttat atttattttg
    15781 ttgggctatg gagctggttt tttaggcgat ttgctttatg cttcttggtt ccaatatctt
    15841 actggggcaa ttattatcct tcttggtttg caccaaatgg agattctaca ctttaagggg
    15901 ctttataagg aaaagagact acaactgcaa ggacaggggc aaaatggtaa gggctatagt
    15961 caggcatttt tattgggctt gacctttagt tttgcttgga cgccttgcgt ggggccggtt
    16021 ctggggtctg ttttggcctt ggcggcttca ggtggttcag gagcttggca gggagctggt
    16081 ctcatgttgg tgtatacgct gggcttggcg ctaccattct tgcttctagc tctgacctct
    16141 agttatgttt tgaaacattt ccgaaaactt catccctatc tcggaatcct caaaaaagtg
    16201 ggtggttttc tcattattgt gatgggactc ttggttctgt ttggaaatgc ttcaatttta
    16261 agtcaattat ttgaataaaa tggaaaggaa tatcaatatg aaaaaatggc aaacatgtgt
    16321 tcttggagca ggttcgctcc tttgtttgac ggcttgttca ggcaagtccg tgactagtga
    16381 acaccaaacg aaagatgaaa tgaagacgga gcagacagct agtaaaacaa gcgcagctaa
    16441 agggaaagag gtggctgatt ttgaattgat gggagtagat ggcaagacct accgtttatc
    16501 tgattacaag ggcaagaaag tctatctcaa attctgggct tcttggtgtt ccatctgtct
    16561 ggctagtctt ccagatacgg atgagattgc taaagaagct ggtgatgact atgtggtctt
    16621 gacagtagtg tcaccaggac ataagggaga gcaatctgaa gcggacttta agaattggta
    16681 taagggattg gattataaaa atctcccagt cctagttgac ccatcaggca aacttttgga
    16741 aacttatggt gtccgttctt acccaaccca agcctttata gacaaagaag gcaagctggt
    16801 caaaacacat ccaggattca tggaaaaaga tgcaattttg caaactttga aggaattagc
    16861 ctaggaggtg tcttatgaat gataagttaa aaatcttctt gttgctagga gtattttttc
    16921 tagccataac cggtttctat gttctattga tacgaaatgc agggcagaca gatgcctcgc
    16981 aaattgaaaa ggcggcagtt agccaaggag gaaaagcagt gaaaaaaaca gaaattagta
    17041 aagacgcaga cttgcacgaa atttatctag ctggaggttg tttctgggga gtggaggaat
    17101 atttctcacg cgttcccggg gtgacggatg ccgtttcagg ctatgcaaat ggtagaggag
    17161 aaacaaccaa gtacgaattg attaaccaaa caggtcatgc agaaaccgtc catgtcacct
    17221 atgatgccaa gcaaatttct ctcaaggaaa tcatgcttca ctatttccgc attatcaatc
    17281 caaccagcaa aaataaacaa ggaaatgatg tggggaccca gtaccgtact ggtgtttatt
    17341 acacagatga caaggatttg gaagtgatta accaagtctt tgatgaggtg gctaagaaat
    17401 acgatcaacc tctagcagtt gaaaaggaaa acttgaagaa ttttgtggtg gctgaggatt
    17461 accatcaaga ctatctcaag aaaaatccaa atggctactg ccatatcaat gttaatcagg
    17521 cggcctatcc tgtcattgat gccagcaaat atccaaaacc aagtgatgag gaattgaaaa
    17581 agaccctgtc acctgaggag tatgcagtta cccaggaaaa tcaaacagaa cgagctttct
    17641 caaaccgtta ctgggataaa tttgaatccg gtatctatgt ggatatagca actggggaac
    17701 ctctcttttc atcaaaagac aaatttgagt ctggttgtgg ctggcctagt tttacccaac
    17761 ccatcagtcc agatgttgtc acctacaagg aagataagtc ctacaatatg acgcgtatgg
    17821 aagtgcggag ccgagtagga gattctcacc ttgggcatgt ctttacggat ggtccacagg
    17881 acaagggcgg cttacgttac tgtatcaata gcctctctat ccgctttatt cccaaagacc
    17941 aaatggaaga aaaaggctac gcttatttac tagattatgt tgattaatac tcttcgaaaa
    18001 tctcttcaaa ccacgtcagc gtcgccttac cgtatgtagg gttactgact tcgtcagttt
    18061 tatctacaac ctcaaagcta tactttgagc tgacttcgtc agttttattt acaacctcaa
    18121 agctatactt tgagaaacct gtggctatct tcctagttta ctttttgatt tttattgagt
    18181 ataagaggtc tttcctaagc agttagagga gaattttgct atactgatac tagtaagtga
    18241 caaaggagag aagcatgacc tacacaatct taatcgtaga agatgaatat ctggtaagac
    18301 aaggtttgac taaactggtc aatgtagcag cctacgatat ggaaatcatc ggtcaggctg
    18361 aaaatggaag gcaggcttgg gaattgatcc aaaagcaggt gccagatatc attttaaccg
    18421 atatcaacat gcctcatcta aatggcatcc agttggccag tctggtacga gaaacctatc
    18481 ctcaggttca tttggtcttt ttaacaggtt acgatgattt tgattatgcc ttgtctgctg
    18541 tcaaactagg tgtggacgac tacctgctca aacccttttc tcgtcaggat attgaggaaa
    18601 tgttggggaa aatcaaacaa aaactagaca aggaagagaa agaagagcag ttacaagatt
    18661 tattgactaa taggtttgaa ggaaacatgg cccagaaaat ccagtctcat ctggctgata
    18721 gccaatttag tttaaagtct ttagccagtg acttaggttt tagtccgacc tatctgagtt
    18781 ccttgattaa gaaagagttg ggcttgcctt ttcaggatta tctggtgaga gaacgtgtta
    18841 aacaagccaa gctcttgctt ttaactacag atctgaagat ttatgagatc gcagagaagg
    18901 ttggttttga agatatgaac tattttaccc aacgttttaa gcagattgca ggtgtgacac
    18961 ctcgtcagtt taagaaggga gaagaccgat gaagcgttct tctcttttag ttagaatggt
    19021 tatttccatc tttctggtct ttctcattct cctagctctg gttggaactt tctactatca
    19081 atcaagttct tcagccattg aggccaccat tgagggcaac agccaaacga ccatcagcca
    19141 gactagccac tttattcagt cttatatcaa aaaactagaa accacctcga ctggtttgac
    19201 ccagcagacg gatgttctgg cctatgctga gaatcccagt caagacaagg tcgagggaat
    19261 ccgagatttg tttttgacca tcttgaagtc agataaggac ttgaaaactg ttgtgctggt
    19321 gaccaaatct ggtcaggtca tttctacaga tgacagtgtg cagatgaaaa cttcctctga
    19381 tatgatggct gaggattggt accaaaaggc tattcatcag ggagctatgc ctgttttgac
    19441 tccagctcgt aaatcagata gtcagtgggt catttctgtc actcaagaac ttgttgatgc
    19501 aaagggagcc aatcttggtg tgcttcgttt ggatatttct tatgaaactc tggaagccta
    19561 tctcaatcaa ctccagttgg ggcagcaggg ctttgccttc attatcaatg aaaaccatga
    19621 atttgtctac catcctcaac acacagttta tagttcgtct agcaaaatgg aggctatgaa
    19681 accctacatc gagacaggtc agggttatac tcctggtcac aaatcctacg tcagtcaaga
    19741 gaagattgca ggaactgatt ggacggtact tggcgtgtca tcattggaaa agttagacca
    19801 ggttcggagt cagctcttgt ggaccttgct tggggccagt gtcacatctc ttcttgtctg
    19861 tctctgctta gtgtggttca gtcttaaacg ctggattgct cctttgaagg atttgagaga
    19921 aaccatgttg gaaattgctt ctggtgctca aaatcttcgt gccaaggaag ttggtgccta
    19981 tgaactgaga gaagtaactc gccaatttaa tgccatgttg gatcagattg atcagttgat
    20041 ggtagctatt cgtagccagg aagaaacgac ccgtcagtac caacttcaag ccctttcgag
    20101 ccagattaat ccacatttcc tctataacac tttggacacc atcatctgga tggctgaatt
    20161 tcatgatagt cagcgagtgg tgcaggtgac caagtccttg gcaacctatt tccgcttggc
    20221 gctcaatcaa ggcaaggact tgatttgtct ctctgacgaa atcaatcatg tccgccagta
    20281 tctctttatc cagaaacaac gctatggaga taagctggaa tacgaaatta atgaaaatgt
    20341 tgcctttgat aatttagtct tacccaagct ggtcctacaa ccccttgtag aaaatgctct
    20401 ttaccatggc attaaggaaa aggaaggtca gggccatatt aaactttctg tccagaaaca
    20461 ggattcggga ttggtcatcc gtattgagga tgatggcgtt ggcttccaag atgctggtga
    20521 tagtagtcaa agtcaactca aacgtggggg agttggtctt caaaatgtcg atcaacggct
    20581 caaacttcat tttggagcca attaccagat gaagattgat tctagacccc aaaaaggaac
    20641 gaaagttgaa atatatataa atagaataga aactagctaa ctcccagtca attctgggag
    20701 ttttatgcgt aattgggcaa actttctagg ttgtctatct tgctaaaaac aagaaaaaac
    20761 ggtatgatag aagagtgaca acagaggtaa cagagatgaa cgagacacag attcaaagag
    20821 aaacacgtca ggtagtggaa gatgttttag aaaagaccaa tttgaaacag ggggcccttt
    20881 ttgttttagg tctatcttct agtgaggtgc taggtggtca gattgggaaa gaatccagtc
    20941 aagagattgg tgagctgata gtggagacta tcttgggtat tctaggtagt agaggtatcc
    21001 atttagcagt tcaaggttgt gagcatgtca atcgggctct cgttgttgaa cgtcaggtgg
    21061 cagagcagtt tggtctggaa attgtcagtg tccatcctac tcttcatgca ggaggttcgg
    21121 ggcagttggc agcctttaag tttatgcagg atccagttga ggttgaattt atcaaggctc
    21181 atgctggatt ggatatcgga gacactgcaa ttggcatgca tgtcaagcat gttcaggttc
    21241 cgattcgccc tattttgaga gagattggtc atgcccatgt aacggcactg gctagtcgtc
    21301 caaaattaat cggaggtgcg cgtgcgcact atccgcaaga tgctattaga aagacctgag
    21361 aatgaataaa ggagataaaa gtgtttaaaa aagaccgttt ttcaattcgt aagattaagg
    21421 gagttgtagg ctctgtattt cttggaagcc ttttgatggc tccttctgta gtggacgcag
    21481 ccacctatca ctatgtaaat aaagagatta tttcacaaga agctaaagat ttaattcaga
    21541 caggaaagcc tgacaggaat gaagttgtat atggtttggt gtatcaaaaa gatcagttgc
    21601 ctcaaacagg gacagaagca tctgttttga cagcttttgg ttatctatca ggtgacatcc
    21661 tcaaaacgct tggcttggac actgttttag aagaaacctc agcaaaacct ggagaggtga
    21721 ctgtagtcga agttgagact cctcaatcaa caacaaatca ggagcaagct aggacagaaa
    21781 accaagtagt agagacagag gaagctccaa aagaagaagc acctaaaaca gaagaaagtc
    21841 caaaggaaga accaaaatcg gagataaaac ctactgacga cacccttcct aaagtagaag
    21901 aggggaaaga agattcagca gaaccagctc cagttgaaga agtaggtgga gaagttgagt
    21961 caaaaccaga ggaaaaagta gcagttaagc cagaaagtca accatcagac aaaccaacag
    22021 aggaaccaaa agttgaacaa gtaggtgaac cagtcgagcc aagtgaagac gaacaggcac
    22081 caacggcacc agttgagcca gaaaagcaac cagaagctcc tgaagaagag aaggctgtag
    22141 aggaaacacc gaaaccagaa gataaaataa agggtattgg tactaaagaa ccagttgata
    22201 aaagtgagtt aaataatcaa attgataaag ctagttcagt ttctcctact gattattcta
    22261 cagcaagtta caatgatctt ggacctgttt tagaaactgc aaaaggtgtc tatgcttcag
    22321 agcctgtaaa acagcctgag gtaaatagtg agacaaataa acttaaaacg gctattgacg
    22381 ctctaaacgt tgataaaact gaattaaaca atacgattgc agatgcaaaa acaaaggtaa
    22441 aagaacatta cagtgataga agttggcaaa acctccaaac tggggttaca gaagctgaaa
    22501 aagttgcagc taatacagat gctaaacaaa gtgaagtaaa ttctgagact gcaagtctta
    22561 aaacagcaat ttctagatta aatacagata aagttgaact cgaaaatcaa cttaagattg
    22621 cacagggaaa aactgaaact gatttcagca tggaatcttg gactgttttg agtacagcta
    22681 aaaataaagc tcaggaagta aaagataacg gaacagcgac ccaagaacaa ataaacgaag
    22741 cagagaaaag tttgaaaaca gcccttgctg acttatctgt tgacaaaaca gctttgggaa
    22801 gcgctatcga tacagctact aagaaaaata aagagaatta tacaaatcaa acatgggcag
    22861 agttggaaac agcgcttaca gcagcaaaat ctgtaaatac taacgagtct aaacaaagcg
    22921 acgttaacga agctgctgaa aaattaactg caactatgga aaaattggtt gaattgtctg
    22981 aaaagccacg tttgactcta tctattgaaa agagagacat agatcgaaaa gctacggtca
    23041 cgtatacttt ggaaaatcca gcaaatactc aaataaaatc aataactgca actctgaaaa
    23101 aaggagagga agttgttaag gattttgttt taacagaaga aaatcttaaa actaatcatt
    23161 taactgcttt gtttgaaaag ctagactact acaaagaata cactctatct acggatatgg
    23221 tatataacag aggaaatgat gatgagacgg agagtatttc agaagaacta attcagctga
    23281 atcttaagaa actagaattg aaagatatcc agacagtctc tctgatgaaa tttgaaaatg
    23341 gtcaagaaag tcaggttaca cacttgtctg ataaaccgac cgatttgagt aaattatact
    23401 taaaagttac atcatcaacg tctaaggatg ctgtgttagc cgttagctct atagaagaag
    23461 agattattga aaataaaaaa atctttaaaa ttcatgcaga tacaccagaa ttagtagttc
    23521 gtaagaaaga tggcagtctt tctaagggat ttgactatta catggagaga gttatccctc
    23581 atgatggtga tatttattat gattttaaag acttgatttc agctatgaca tctaatccta
    23641 caggtacgtt tattttaggg agagatattt cctcgcgtaa tgtaaaaccg gatggaaatg
    23701 gaaaatctta tatcaaaggt gaatttaaag gaaaactact tggtacaaat gataatgtta
    23761 gacactcgat ctttgactta gaatatccat tgtttgatac tataaaatca ggagtcgtga
    23821 aagatattga tttcaagcat gtcaatatgg tattcccaga tagcaatcaa ggagataacg
    23881 tagcaacaat tgcaagagta atcaaagata agacaaaaat tgagaatgtt aatgttgaag
    23941 gttatctcga gggtcgagat catgtagctg gtttagttaa taatcttgaa ggtaattccg
    24001 aaattgaaaa tgtatccttt acaggaaaga ttaagtctaa aggtggtaat tctattacag
    24061 caggtattgc aggtagaaat atcttatctc gtgttaaacg tgcctatgta aatgcagata
    24121 tagaggtaca tcgatcatct aattctagca tgcttgttgc agtaaatgga ataaacgcag
    24181 atgcaagtgg tggttgggga acctggggta gacttactga gtcagttgct aaaggaacat
    24241 tagaaaccaa gcaaggaggg caagctggag gagcctcttc tacagtctgg ccttatggag
    24301 caattgataa tgttgtcagc tatgcaaaag tgaccaaagg gaaagaatta tttggatcag
    24361 atggtgattt aaattatgat tggtttatga aaaaaattag taatattttt ggtgttcaag
    24421 gcatcagctc aggcgattcg ggtagtgatt ctaaatttac gcgtatttct gaggaagaag
    24481 caaatcaaaa agttgcttct tataatatta cagctcccaa cttgatgagt gactcaagtc
    24541 ttttggtaga cagactcaat gaaagttgga aaaatactga tcaatttgaa agtatacagg
    24601 attatcaagc ccaaaaccaa ttaatttacc agaacttgac taaatttact ccttattaca
    24661 ataaagaatt tattgttcat gaaggaaatg ctctcactcc tgaacaggaa atattgaaga
    24721 ctaagaaaat caaatcgatt gtagggttga aaggaacaga gttcgttgta gatggcagtg
    24781 atattgatac tatcatgctt cattttgagg atggtagtca gaagcgatat aaggtaacct
    24841 ctactggtaa gttttcgata actaatttac cggaatatca ggttgaagat ttaaatgtgg
    24901 tgtatacatc agaacatata gtacatccac tagattcgtc actgataaat aaccttgttg
    24961 aagaattgaa aaaagtagaa ttgtatacag agtctactta ccaagttcta ggtattgata
    25021 aagataatgc taacaaattg aatagaacta agagattatt cttagatgag tcattagatg
    25081 ctgttaaaac acaattgcct acctttgtaa aaacaatgtt tgaaaatgag tggttgcaca
    25141 tcaatggaga atctagtgga gctgtcgctg ctttacgaca aaaaatcatg gataataaga
    25201 cggctatatt gcttgctttg acttatatca atcgttatta tgatgttaaa ttctcagact
    25261 ataatattaa aaaactgatg ctctttaaac caactttcca tggtgaaaag attgatctat
    25321 tagatcgatt aatccgacta ggatcatctg gagaaaatag attaaaaggt tcagaaaatg
    25381 cagaaacgtt caagcagtta tttgctagcg aaacgaagca gaaggatttg gtgacttatt
    25441 tagactacaa tcgctctttg ttgactaatt atcaaacaac aggggaatgg tttaaagaaa
    25501 cgactaagga ttatattcaa tttgaggaac gtccatcctt agtagaggag attaaagatg
    25561 ccaaatatcg agtttacgat aatttaaccg caccgtatta tcaaggatat atcctacctc
    25621 tcttaacgtt gaagaatact catttagcaa ttttatcgaa ctatagtact atgacatttg
    25681 tcagccgtga aaaacgccct aattggaaaa atgaagattt tgataagtgg gtgaaatatg
    25741 tagcaactgc tcatagaaac catgttgata cttggtataa gattttacct gataatatca
    25801 aaggtaaaat ggttaaagag aatgttactg ctgtttggga aggattgagc atcccaggaa
    25861 gtgaatgggt agaccagaat gctgtagata gaaaaggaag agattacgcc ccagctcgag
    25921 aattctttaa tcttgtaggt ggtcctatgg gaggttggta tgcttaccat ggttatggtg
    25981 cgcatgctgg aggaagaaat cgggttaatt atgaggtgtt tgatgtatta agtgagtatg
    26041 gtatatcggt ctttactcat gaattgactc acgttaatga tacttggatt tacttaggag
    26101 ggtatggtag acgtgaaaat atgggacctg aagcttatgc tcagggatta ttccagtctc
    26161 ctgttccagg tcagccgggt tggggggcgc ttggtctcaa tatggcattt gaaagaaaaa
    26221 atgatggtga tctaatttat aatgcctccc caactcaatt tgaaaatagg aaagagttag
    26281 atagttatat gaaaaattat aacgatactc tcatgatggt ggactacttg gaaggagatg
    26341 cagttatctc taaaggtaaa gaagctatta ctaaatggtt taaaaaagtt gaacctaagg
    26401 ttgttagtca aacagctcag tatgatacgg ttcgtcagtt gactgctgaa gagaaagaaa
    26461 aactttctgt ttctagtgtt gatgatttag ttgatcaagg attaatgtca gacagagctg
    26521 taggaaataa tacctataat ccagctgatt ttgaaacaag ttatattgcg atagattata
    26581 tgactggtat ttatggtgga ggaaagaata gtgtaggttc accaggtgcc ttgatgttta
    26641 aacataatac tttccgtatg tggggctact atggttttga agaaggtgtt ctaggatatg
    26701 cttctaataa atttaagcaa gcatctagag atgaaggtca tgctggactt agtgataact
    26761 ttattatttc taagatttcc aaaggggaat ttttaactat ggaagcattt aaaaagggat
    26821 attttaagaa agtagtagaa gagttaaaaa ctaaaggtat tcgtcctgtt actatcaatc
    26881 aaaaaacata tagcaccttt gaagaattgc aagaaggatt taagcaggct gttgaaagag
    26941 atttgaagaa aaatcagtta gatgagaggg aaacaagaaa cttcaaattc caagttttca
    27001 gacaacttct tcaacaaaca gatagcttta aaacttctat ctttagataa tcgaaatctc
    27061 tcatctgctt tgcggatgag ggttttctta ttttatgcta tacttaaagt atgcatagaa
    27121 aaacagtgat tgattttagg actttggggg agagatatac ctttacccag cctattaaag
    27181 agttgaaaac gagaaatgta gaagaagtgg cagatttgct ggcacaagtg gaaagctacc
    27241 aagagcaagg ttattatgtg gtggggtatg tcagctacga ggctgcacct gcttttgagg
    27301 aaaaattagc agttcacaag gatcccctac tgggagagta cctgctttat tttactgttc
    27361 atgatagcgt agaaacttcc cctattcctc tgacttatga ggatattgat ttgccctcaa
    27421 attggcagga agtaacgtct gcagcagact atgaaaaggc cattgcccaa attcaccatc
    27481 atttacggca gggagatacc tatcaggtca actacaccgt ccaacttaag caaaagttaa
    27541 atgccaatcc ttttgccatc tacaatcgta tggtggtaga gcaggaggcg ggctacaatg
    27601 cctatgtgga acatgacgag atggcagtga tttccatgag cccagagctc ttttttgagc
    27661 aaaatgatcg cgagttgaca acacgaccaa tgaaggggac gactcagcgt ggggtaactg
    27721 accaagaaga tcttgaacag gccagttggt tggaacagga tcccaaaaat cgctctgaaa
    27781 atatgatgat tgtggacctc ttgcgcaatg atatgaaccg tatttctgaa gttgggagcg
    27841 agcacgtgga gcgtctgtgt caggtagagc agtattcaac tgtttggcag atgacttcga
    27901 ccatcaagag tcagttgcga gaggatgtgg accttgttga aatcttccac tcactctttc
    27961 cttgtggttc cataacgggt gcaccgaaaa ttgcgacaat ggagattatc aaggacttgg
    28021 agcctcaacc gcgtggagtc tactgtggaa cgattggtct cttgcttcca aatggacgac
    28081 ggatttttaa tgtggccatt cgtaccattc aacttcacaa aggtcaagcc atttatggag
    28141 ttggcggagg gattacttgg gatagcactt gggaatctga ataccgtgaa gttcatcaaa
    28201 aggcagcagt tctctatcgt aaacaagctc gcttccaatt aattacaact ggaaaaatca
    28261 gccaaaaaca actgatgttt gaagaacaac atctggaaag gctgagaaaa gctagtcggt
    28321 attttgctta tccttttgac ccagaaattt tgagacaaaa gattgaagca gagtgtcaga
    28381 cttgtgatgc taatcaagat taccgcttgc gaatttctct cagcaagtct ggagagatag
    28441 aattcagtcg tcaaatctta acacctctta gtccaagttt ttgtcaggcc aaactttgtc
    28501 tgcaagaagc gaatttgaat caatccttta cctattttaa aacgacttac agaccgcatt
    28561 tgagcataga ggaacaagag aagatttacc ataatggaaa aggagaactt cttgaaacct
    28621 ctatcggaaa tttggttctg aaaatcgatg ggaaactcta cacaccgcct atccgacttg
    28681 gaatcttgcc aggaatttat caccagtatt tactagaaac aggacaggta gaagagaaag
    28741 tcttgacctt ggcagatttt gcccaagcag aagctattta cggctgtaat gcagtgagag
    28801 gcttgtatga gttgcaagtg ataggattgt gagttttagc tattaatagt ttttaatata
    28861 tattaaaata tattggtgtt tacttttgat gagatatcat atttgtgtta taatgtaacc
    28921 gtaaacaata gaaatttaaa ggagattttt gatgaaatca aaaatagttt tgggagcttc
    28981 gctagcgatc gcaacactta gcttagtatc tcttgtcgag attgaaggac tctcaccgtt
    29041 tttaatagag aatgttagcg caaatactca ttctgcaaat aaagttatta atcataaagt
    29101 aagtatttac ttagaaaatg ctgatgaagg taaaggtcta actgtaaact tttcaacaga
    29161 cagtgttagt ccaaatttat ttgatgagtt cgaaaaaaag agtggaatta caataactac
    29221 tatgttagtg aatgctaaga caggtgaagt tgtagaaaaa agattaacac caagcgtttt
    29281 tttacgttcc aatgatttaa ccagtgggac catatcttcg tttattttta gtgaatatcc
    29341 tgatggagaa tataaatatg ttgtgtctaa aggagacttt atcgatccta agactcagtt
    29401 taaacatcag tatagaggag aatctccagt ttttagaata cgtaatcgaa aatatgtgga
    29461 gttgggaaca actgataaaa aattagatga aagacgtgat aatagcgttt ataaagatgg
    29521 ggttgttgaa cacaaggtaa atttaagttt aacaagttat caaggaggta atggagttac
    29581 tgcaattttt tcaactgata gtgttaactc taacttactt aattcttttg gtgaaaaggc
    29641 taaaaaagtt ttgattaggt ctaagttaat taatgttaag actggcgagg ttattgatga
    29701 aacgttttct ccaaaagtaa gtttaacctc taaaattttg aaaagtggtt ctacagcagt
    29761 attttatttt atagatttaa ctgatggcga atataaatat gttgcgtatg aaagccagca
    29821 atatactgat cctcaaacaa cattgactca tcagtataga ggagaatctc ctatttttag
    29881 tatcaaagat ggaaaatttt ctggtttagt atctgcctca aaaccagacg aaaacccgaa
    29941 gccaacgcca aaaccggatg agaaaccaaa accttcagca ccacaacagg agaaaacaaa
    30001 acctacagtt cagtctggtt gggtaggttc atcttattat caaaatggta aaaaagtcac
    30061 tagtaagtgg atttttgata aaaaatataa ttcatacttc tatctagatg catcaggaaa
    30121 ttatgttcaa aacgcttggg taggtaacta ttatctcaaa tcaggcggat acatggctaa
    30181 aagtgagtgg atttatgata agaactatgg ttcatactac tatctaacat cagaaggcag
    30241 ctacgctcgt aacacttggt caggcaatta ttatttgaag tcaaatggta agatggctaa
    30301 aagtgagtgg gtatacgata gtaactacaa atcatattac tacttgacat cagaaggaag
    30361 ttatgctcgt aacacttggg taggcaatta ctatcttaaa tcaaacggta aaatggctgt
    30421 taatgaacgt acaccagatg gttatcgtgt agatggttca ggtaaatggg ttaaataaga
    30481 aactaaaaaa taggataata ggtaatatca gatgaataaa aaaaattatt ttcatgtgct
    30541 ttagcatttt caacgattgg aacattgtca acgattgata aatcaggagt tcttcctttt
    30601 tcagcgcaaa cggtgcaggc tgacatacaa acgcttaccc caatggcaaa atattatatt
    30661 attcttccga aggatgcaga aatatataaa acttggagag gtactgtcaa tattcccatt
    30721 attgatgcta caaaaactac tccagaatta tcttatttca aagaagatca tcgcaactat
    30781 atagctaatg agaataagtc tggtgccaat tacatagagt ggaagggaac agtagaagag
    30841 tttaaagagg ccattaagaa gttgactgat aaaaaatcaa ctactgcaac accaaaaaag
    30901 gatgaaaagc caacaccaaa accggatgag aagccaaaac caacacctac agttcagtct
    30961 ggttgggtag gttcatccta ttatcaagat ggtaaaaaag tcattagtaa gtggattttt
    31021 gataaaaaat ataattcata cttctatcta gatgcatcag gaaattatgt tcaaaacgct
    31081 tgggtaggta actattatct taaatcaggc ggatacatgg ctaaaggtga atgggtttat
    31141 gatgccacct atcaagcttg gtattatttg acatcagatg gttcttatgc ttacagtaca
    31201 tggcaaggaa attactatct aaaatcggat ggtaaaatgg ctgtcaatga atgggttgat
    31261 ggtggacgtt attatgttgg cgctgacgga gtttggaagg aaggtcaagc aagtacagct
    31321 tcttctagta atgatagcaa tagtgaatat tctgctgctt taggaaaggc aaaaagttat
    31381 aattcgttat tccacatgtc aaaaaaaaac gtatgtatag acaattaact tctgattttg
    31441 ataaattttc aaatgatgca gctcaatatg ccattgatca ttagatgatt aacagatact
    31501 ggtaggcgaa aaaattcctt ataattcatt tacgcaaacc tttgcataag tgaatggatt
    31561 ttgatatact agtactgtaa gcattttcaa ttactacaca atgaaaaagg agaatatgat
    31621 gagtcaaaag attattggga ttgaccttgg tggaacttct atcaaatttg caatcttaac
    31681 aacagcagga gaaatccaag gaaaatggtc aatcaagacc aacattttgg atgagggaag
    31741 tcatatcgtt gatgatatga ttgagtctat tcaacatcgt ttggacttgc ttggattggc
    31801 agcagcggac ttccaaggca ttggaatggg atcaccaggt gtggttgacc gtgaaaaagg
    31861 gactgttatc ggtgcctaca acttgaactg gaaaaccctt caaccaatca aacaaaagat
    31921 tgaaaaagcc ttgggcattc catttttcat cgataatgat gccaacgtag cagctcttgg
    31981 tgagcgctgg atgggtgctg gagataacca accagacgtt gtctttatga cactcggtac
    32041 tggtgttggt ggcggtatcg tcgcagaagg caaattgctt cacggtgttg ctggtgcagc
    32101 aggtgagttg ggccacatca ctgttgactt tgaccagcca attgcatgta cttgcggtaa
    32161 gaaaggctgc cttgagacag ttgcttcagc aacagggatt gtcaacttga ctcgtcgcta
    32221 tgccgatgaa tacgaaggcg atgcagcctt gaaacgcttg attgataacg gagaagaagt
    32281 aactgctaag actgtctttg agctcgcaaa agaagaagac gaccttgctt tgattgttta
    32341 ccgtaacttc tcacgttact tgggtattgc atgtgctaac attggttcaa tcctaaaccc
    32401 atcaacaatc gtcatcggtg gtggtgtgtc agctgcagga gaattccttc tacaaggtgt
    32461 tcaaaaagtc tacgatgaaa atagtttccc acaagtacgc acatctacta aattagcact
    32521 tgcaactcta ggaaatgacg ctggagttat cggagcagca tcacttgtat tgcaataaga
    32581 taatgagaga ggaaaatgct aacctagagt tagtaatgtt cctcttttat tttttatgct
    32641 atactagtta ggacaatgaa tgaggtgaaa cgaaatgaca aaagcagata cgatttttaa
    32701 agagaatatt gaacgcattc taaaggaagg tgttttctca gagcaggctc gtcctaagta
    32761 caaggatggg actgttgcta attccaagta tgtaactggt gccttttccg agtacgactt
    32821 atctaaagga gaatttccta tcacaacctt gcgtcccatt gcaatcaaat ccgccatcaa
    32881 agaagttctc tggatttacc aagaccagtc aaatagctta gaagtgctta atgacaagta
    32941 caatgttcac tactggaatg actgggaagt tggagacacg ggaaccattg gtgagcgcta
    33001 tggtgccgtt gttaagaaac acgacattat caataagctt ctcaaacagt tggaaaccaa
    33061 tccttggaac cgccgcaata ttatttcgct ctgggattac caagctttcg aagaaacaga
    33121 tgggctgctc ccgtgcgcct ttcagaccat gtttgatgtc cggcgtgttg atggggaaat
    33181 ctatctggat gcgaccttga cccagcgctc caatgatatg ctggtggccc accacatcaa
    33241 cgctatgcag tatgtggctt tgcagatgat gattgccaaa cattttggct ggaaggttgg
    33301 gaagttcttc tacttcatca acaacctcca tatctatgat aatcaatttg aacaagctca
    33361 ggaattgctc cgtcgggagc cgtcaaactg ccaaccacgc ttggttttaa atgttcctga
    33421 tgggactaat ttctttgata tcaaagcaga agattttgag ttggtggatt atgaccctgt
    33481 taagccacag ttgaagtttg acctagctat ttaaaagaat agaaaaaaga agttgagaat
    33541 aatcccaact tcttttgttt cttaacgtga tacgcggcga cgagctgctt ttttacggtt
    33601 ttcttcgatg aaagctgctt tttgctcttc tggttcgatt actttctttt taaatgcgta
    33661 tactgcacct gcaacggcag cgacagttcc tgcgacacct gttacaagac ctttagcgaa
    33721 tcctttagcc atgagtcttc ctcctttata ttctcaatca gccagcctcc tcaagaggtc
    33781 acatttttct gactgacctt tttgtgttat aataatagta acgaaaaaat gggaattttt
    33841 caaggaaaaa agatgaaaac aaaaataatt gtgattgttg gaccgactgc tgttggaaag
    33901 acggctctag ctattgaagt tgcaaagcgc tttaatggcg aagtggttag tggagatagc
    33961 cagcaagtct atcgaggact tgatattggg acggccaagg ctagtccaga agagcaggca
    34021 gctgttcctc atcatttaat cgatgttaga gagataaccg agtcttactc ggcttttgat
    34081 tttgtttcag aagctaagat gactattgag gatattcaga gccgtggcaa gctagccatt
    34141 atcgccggtg ggactggact ttatatccag agcttgctag aaggttacca cctaggtggg
    34201 gagactcctc atgaggagat tttagcttat cgagctagtt tggagccata ttcagatgag
    34261 gaattagccc atttggtgga gcaagcaggc cttgagattc cccagtttaa tcgtcgtcgt
    34321 gctatgcgtg ccttagaaat tgcccatttt ggtcaggatt tggaaaatca agagatcttg
    34381 tatgaaccgc tgattatctg cttggatgat gaacgtagtc aactttatga gcgtatcaac
    34441 caccgagtgg atttgatgtt tgaggctggg cttttggatg aggccaagtg gctgtttgac
    34501 cattccccta atgtacaggc tgctaaagga attggctata aggaactctt tccttatttc
    34561 cgtggagagc agacctttga ggaagctcgt gagagtctta aacaggcgac ccgtcgtttt
    34621 gccaagcgtc agctgacctg gttccgtaat cgcatgcagg tcacctttta tcagattgga
    34681 gaatctggtg tacaagaccg cattttaagc cagatagagg agtttttaga tgattgaaac
    34741 ggagaaaaaa gaggagcgag tcctgctgat tggtgtggaa ttgcagggta tggacagttt
    34801 tgacctctcc atggaagaat tggctagttt agcgaaaacg gcaggggcag tcgttgtaga
    34861 tagctacaga caaaaacgtg aaaaatatga ttccaagacc ttcgtcggct ctggtaagtt
    34921 ggaagagatt gcgcttatgg tggatgcaga agaaatcact actgtcatcg tcaacaatcg
    34981 tctgacccca aggcagaatg tcaatctaga ggaagttctc ggtgttaagg tcattgaccg
    35041 tatgcagttg attttggata tctttgccat gcgggctcga agccatgaag ggaagctcca
    35101 agtccaccta gcccaactca aatacctctt gcctcgcttg gttggtcagg ggattatgct
    35161 cagccgtcag gcagggggaa ttggttcccg tggtcctggt gaaagccaac tggagctgaa
    35221 ccgtcgtagc gttcgcaatc aaatcacgga tatcgagcgc cagcttaagg tggttgagaa
    35281 aaatcgtgcg actgtcagag aaaaacgttt ggagtctagc acttttaaga ttggtttgat
    35341 tggttatact aatgctggga aatcaactat catgaacatc ttgaccagta agacccagta
    35401 tgaagcagat gagctctttg cgactctgga tgcgacaacc aagagtattc atctgggagg
    35461 caatctccaa gtaactttga cagataccgt tggctttatc caagatttgc cgacagagtt
    35521 ggtgtccagt ttcaagtcaa ccttggaaga aagcaagcat gtggaccttc tggttcatgt
    35581 tatcgatgct agcaatcctt accacgagga gcatgaaaaa acggttctct ccatcatgaa
    35641 agacctggac atggaagata ttcctcactt gacgctttat aataaagcgg atttggtgga
    35701 ggatttcacg cctacccaaa cgccatatac cctcatttct gccaagtctg aggacagtcg
    35761 tgaaaacttg caagcattat tgctagataa gattaaggaa atttttgaag catttaccct
    35821 gcgagtgcct ttttcaaagt cctacaagat tcatgattta gagagtgttg caattctgga
    35881 agaacgtgat tatcaggaag acggcgaagt gattacaggc tacatttccg agaaaaataa
    35941 atggaggtta gaagaatttt atgactgata ttaaaacttt ggctctaaag tatggaggtt
    36001 atacaagtct ggacaaggtc tatctggatc agcttctagc tggtaaaaca gatcaggagc
    36061 agttaacact tattacacct ccgccaagtg ttgttaatgc ctactttgca gaactctatc
    36121 agaaaaagag tcctcaggct gcgacggatt attttgcaga actcagtcag gaactgaatc
    36181 tttacaatac tgagccaagt ttcaccctag aaaataagcc ttttattcgg cttaatctgt
    36241 ctggtaaatc ctttggtttt tgttatgaga gtgacggcct tggtcggatt ttctttgaaa
    36301 atgaggagaa aatctcggat gacttgctct ttgaaattgc gcaaattttc ccccatcaat
    36361 tagtctttga agagtctggt aagatttaca tgaagcctgt cgaggacgag gaagttatca
    36421 gcctagaaag tctcacagct ttgactgatt tggaaagatt ggctgatggt cgaaaacgtc
    36481 tcaaaggcta cagccaagag gatttattgc aagaagctgc tgctttttct ggcaaacgct
    36541 atttccgatc ggaaaaccgc acagccatgt tatatattga ttaattagaa agtatcgaat
    36601 ggatattcaa tttttaggaa cgggggctgg tcagccctct aaagcccgca acgtttcaag
    36661 tctcgccctg aaacttttgg atgagattaa cgaagtttgg ctctttgact gtggagaagg
    36721 tacgcaaaat cgcattctgg aaaccacaat tcgaccacgt aaggtcagca aaatctttat
    36781 tacccatctg catggagacc acatttttgg tttgccaggt ttcctttcta gccgtgcctt
    36841 tcaggccaat gaagagcaga cagatttgga aatctacgga cctcaaggaa tcaagtcatt
    36901 tgtcttaacc agccttcgtg tgtcaggttc tcgtctgccc taccgcattc atttccatga
    36961 gtttgaccaa gattctctgg gtaaaattct tgaaaccgat aaattcactg tgtatgcaga
    37021 ggagctggac cacactattt tctgtgttgg ctatcgtgtc atgcaaaagg atctagaagg
    37081 gacgctggat gctgaaaaac tcaaggctgc tggtgttccg ttcggcccgc tttttggtaa
    37141 aatcaaaaac ggccaggatc ttgttttgga agacggaact gaaatcaagg cagcagacta
    37201 tatctcagcg ccacgtccag gtaagattat cactatttta ggagacactc gaaaaacggg
    37261 tgccagtgtg cgtctggctg tcaatgcaga tgtcctagtt catgagtcca cttatggcaa
    37321 gggtgatgaa aaaattgctc gtaaccatgg tcactcaact aatatgcaag ctgcacaagt
    37381 agcggtagaa gcaggtgcca aacgcctcct actcaaccat atcagtgccc gtttcctctc
    37441 aaaagatatt agcaaactca agaaagacgc tgccacaatt tttgaaaatg tccatgtggt
    37501 caaagacttg gaagaagtgg aaatctagca gtcacagaaa ggataagtat gcctactatt
    37561 ctcattaccg gagctagcgg tggtctagct caagaaatgg tcaaactcct gcccaatgac
    37621 caactcatct tgcttggtag aaataaggaa aaattagccc aactctacgg aaattattcc
    37681 catgcagaat tgattgaaat tgatattacc gacgattcag ccctagaagc tctggtaact
    37741 gatctttatc tccgctatgg caagattgat gtcttgatta acaacgctgg ttacgggatt
    37801 tttgagggat ttgaccagat tgctgataaa gatattcacc agatgtttga ggtcaatacc
    37861 tttgccctga tgaatctgtc tcgtcacctt gcggctcgta tgaaggaaag cagcaaaggg
    37921 catatcatca acatcgtcag catggcaggt ctaatagcta ctggcaagtc tagtctttac
    37981 tcagcgacca agtttgcggc tattggtttt tcaaatgctc tgcgactcga acttatgccc
    38041 tatggagtct atgtgacaac agtcaatcca ggtccaatcc gaacaggatt tttcgaccaa
    38101 gccgacccag atggaactta tcttaaatcg gttgaccgct tcctgctaga accagatgca
    38161 gtggctaaaa agattgtcaa gattatagga aaaaacaaac gagaactcaa tctcccgatt
    38221 ttgttgaacc tagctcataa gttttatacc ctctttccta agctagctga taagttggca
    38281 ggggaaactt ttaattataa gtaaaaagaa ccaatgtgca ggttgttgct agcctacata
    38341 ttggttctta tcatttctca actatcaaac tgaacttctt ctaggagata gtctataaag
    38401 cgttcgccca tcttagatag gctggttttc tcatgctgga tatagaccag ctcgatcggg
    38461 tcatcaatat ccagtgggat agaaacgata ttgtctccgt ttaggttgct gttcaaaatc
    38521 cctgtcgcaa tggtataacc atccaaacca atcaagagat taaagagggt ggcacggtca
    38581 ctgaccacaa tggatttctt gtggtgttct tgagaaagaa tctcttctga aaagtagaag
    38641 gagttgtgcg tcccttggtc atagctgagg taagggaaat tctccaaatc agacagtttc
    38701 accttgtctt tctttgccag agggttggtc ttgctgacaa agatatgtgg ttgcgctgtg
    38761 aagagatggt gggctagcag gtgattgtca tccagcatct tggttaaaac atcacggttg
    38821 taactgttta agaagaggac cccgacctca ctgcggaagt tcttgacgtc gtcgataatc
    38881 tcccaagtcc gagtttcacg aaggaagagt tcgtatttct ccatatcgct tttcttgagc
    38941 aaagagacaa aggcattgac cacaaaggca tagtgttgag acgaaacgct aaatagttcg
    39001 cggtgggcga caggattttt atagcgttcc tccagaagct gggtctgctc gacaacctga
    39061 cgggcataag agagaaactc catcccatca cgggtcaagg tgattccctt gggattgcgg
    39121 ataaagatct caatgcccat ttcattttcc aaatctcgca ctgcattgga gagacttggc
    39181 tgagtgataa agagttgctt ggctgcctca ttcatggagc cagtttcgac gattttgata
    39241 atataatgta attgttgaat tctcatgttt ttattgtacc atacttgcct tagaatcgct
    39301 aaagaagata ggaaaataag gagcattgtg tgagtctctt cttgaaaaaa acacaaaatt
    39361 ttgctagaat gagtcttatg aaaacattct atgatgtgca gcaattcctc aaacgatttg
    39421 gtattattgt ttacatggga aaacgcttat atgatattga actgatgaag ttggaactat
    39481 ctcggattta cgatgcaggg ttgatggata aattagacta tctagaggca gaagcagttc
    39541 ttcgcagaga gcacaaggta gaattggatt atattgagaa aaatggagaa aagaactaat
    39601 ggttacttgg attttgtggg cacttatact agcaatgttg gcgtggatgg gctttaacta
    39661 tcttcgtatt cgccgtgcgg ctaaaattgt ggacaatgag gagtttgaag ccttgattcg
    39721 tacgggtcaa ttgattgatt tgcgcgaccc agcagaattc cacagaaaac atatccttgg
    39781 tgcacgcaat attccttcaa gtcagttgaa aactagtctt gcagcccttc gtaaagataa
    39841 acctgtcctt ctctacgaga accaacgtgc gcaacgagtt acaaatgcag ctctttactt
    39901 gaaaaaacaa ggtttttctg agatttatat cctttcttat ggcttggatt cttggaaagg
    39961 gaaagtgaag actagctaac cttatttcca aattagtcga atgataggat gaagctattg
    40021 ttttatagta taattaattt gtataaaatt taaggagttc ttagaacata aaagaaaaga
    40081 agaaaaatcc actttttgtg ggtatcttgt cgattattct tggtttgtta tttccaattg
    40141 taggcctgat tt
//
LOCUS       DAVLWA010000010        38757 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLWA010000010 DAVLWA010000000
VERSION     DAVLWA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.010"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(254..1402)
                     /locus_tag="VYN80_000485"
     CDS             complement(254..1402)
                     /locus_tag="VYN80_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1540442.1"
                     /translation="MKYNKTKYPNIFYYETAKGKRYYVRRSFFFRGKKREKSKSGLTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSTLLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(1581..2504)
                     /locus_tag="VYN80_000486"
     CDS             complement(1581..2504)
                     /locus_tag="VYN80_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEV1540443.1"
                     /translation="MSYSYVALDVETANDFRGSVCSIGLVKFKDGNIVDAFYTLINPE
                     EEFDDFNIFIHGITPEDVLDSPTFPEVRKAIVDFIGSDIVVAHFAQFDMGALKDVYQK
                     YELDFDNIEYICSYRLAKVALPGQLNYKLKRLAKNLNIELDHHNALSDARASGLILEY
                     LLSTNSFSDLNAFLKEYSYNKTGLLGQYGFKRKKSYQYKENLIYQPTEEEKAAMNPDH
                     YFYGLYFCFTGKLERMTRKEANKATALVGGIPEKGVTKHTNILVVGEQDWRVVGTDGL
                     SSKMKKAQTLLEKGQDIEIMTENDFIRLLEE"
     gene            complement(2517..2900)
                     /locus_tag="VYN80_000487"
     CDS             complement(2517..2900)
                     /locus_tag="VYN80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1540444.1"
                     /translation="MTEKEIISHFQIRIIDFDGDLMPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDAIESLDDPTEFDYLK
                     FMSYYNLKTMTNEVMVKEEYFALME"
     gene            complement(2913..3275)
                     /locus_tag="VYN80_000488"
     CDS             complement(2913..3275)
                     /locus_tag="VYN80_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540445.1"
                     /translation="MFFTFEKIKELADKQGISLNKLEEKLGFSRNTIYNMKKSTPNVE
                     RVSMIADYFNVSTDYLLGRTDNPTIAGSHDYKWEGKTLNVEEMASNVMMFGGRELTDE
                     KKKIIQSIIEGYLKEAGD"
     gene            complement(3653..3874)
                     /locus_tag="VYN80_000489"
     CDS             complement(3653..3874)
                     /locus_tag="VYN80_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540446.1"
                     /translation="MSKLTKEDVLQVSQEIINDAIPVIQDMLDEVFKEYPIDMEIRKA
                     ILNSVLVAHKLSTETTVSLLTELVNAQEN"
     gene            3993..4139
                     /locus_tag="VYN80_000490"
     CDS             3993..4139
                     /locus_tag="VYN80_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540447.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFKQASHEIELRALES
                     ETKK"
     gene            4151..4354
                     /locus_tag="VYN80_000491"
     CDS             4151..4354
                     /locus_tag="VYN80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540448.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYG
                     KGSESVKEKVADVLGIENPWRNH"
     gene            4497..5210
                     /locus_tag="VYN80_000492"
     CDS             4497..5210
                     /locus_tag="VYN80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV1540449.1"
                     /translation="MNEIFNFHGQEVRTLTIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQVTMLEDIDYLKSEQPIHPSYAQSLLKKRKA
                     RVVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNISRYDLLPKKFADAALAYWMTW
                     EPSTNTKMKIMKLNSFDDV"
     gene            5224..5481
                     /locus_tag="VYN80_000493"
     CDS             5224..5481
                     /locus_tag="VYN80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1540450.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            5567..5887
                     /locus_tag="VYN80_000494"
     CDS             5567..5887
                     /locus_tag="VYN80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540451.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPQGLRPEPLALPEPKQSA
                     IRFL"
     gene            5903..6196
                     /locus_tag="VYN80_000495"
     CDS             5903..6196
                     /locus_tag="VYN80_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540452.1"
                     /translation="MEELIEWLETKEKLNREMISDEETSDFNLHLENDNDTMFMIKEY
                     LNDYNKLAKDYRILSSENRLLKLEKMELEGRHIYEDMRMKYRANRRKWGARYV"
     gene            6189..6995
                     /locus_tag="VYN80_000496"
     CDS             6189..6995
                     /locus_tag="VYN80_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1540453.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNKNRLDK
                     DKNKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENDVDLVKEALKTAI
                     NSGKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            7135..7905
                     /locus_tag="VYN80_000497"
     CDS             7135..7905
                     /locus_tag="VYN80_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1540454.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            8114..8788
                     /locus_tag="VYN80_000498"
     CDS             8114..8788
                     /locus_tag="VYN80_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1540455.1"
                     /translation="MMKFLNGDCMDIMKQYPDNYFDLAIVDPPYFSGPEKRKFYGRKI
                     SPIGVSRLYGEISEWKIPNRDYFDELFRVSKNQIIWGVNYFDYSFGSGRIVWDKVNGQ
                     SSFSDCEIAYCSYHDSTRLFRYMWNGMMQGKSISEGHIQQGNKALNEVRIHPTQKPVN
                     LYLWLLQTYAKEGDKILDTHVGSASSLIACGELGFDYVGCEIDKSIFNLAQQRLDAYE
                     KQLKLF"
     gene            8798..9025
                     /locus_tag="VYN80_000499"
     CDS             8798..9025
                     /locus_tag="VYN80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540456.1"
                     /translation="MLELYFVYNGHCKFYLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            9152..9319
                     /locus_tag="VYN80_000500"
     CDS             9152..9319
                     /locus_tag="VYN80_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540457.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            9306..9515
                     /locus_tag="VYN80_000501"
     CDS             9306..9515
                     /locus_tag="VYN80_000501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540458.1"
                     /translation="MKYDKQAEIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRRKAEELEDDGRVKEKS"
     gene            9487..9804
                     /locus_tag="VYN80_000502"
     CDS             9487..9804
                     /locus_tag="VYN80_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540459.1"
                     /translation="MMEELKKKVNAVYNWTVEDGKPQPPQQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            9806..10315
                     /locus_tag="VYN80_000503"
     CDS             9806..10315
                     /locus_tag="VYN80_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1540460.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCEG
                     IDIEEVENE"
     gene            10308..10898
                     /locus_tag="VYN80_000504"
     CDS             10308..10898
                     /locus_tag="VYN80_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1540461.1"
                     /translation="MNKDLIETPRFNFFIGDEVLLKGKIVGFDVDENKCVENVVRLEY
                     GQTLNVPNNNIYITDDIVDKSKIKVVVPQFVADWYEENKDSFEFNVCDWIAFRDEAKK
                     SENREFNNWINNSRENPIQTLVNMNQFGYEVEEEKRYLVTLKNRQPLVKSQSGSTLYF
                     SQDITARNYKGTQKELEDANFGWVFDCPGIEIEEVE"
     gene            11056..11316
                     /locus_tag="VYN80_000505"
     CDS             11056..11316
                     /locus_tag="VYN80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1540462.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVML"
     gene            11313..12395
                     /locus_tag="VYN80_000506"
     CDS             11313..12395
                     /locus_tag="VYN80_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1540463.1"
                     /translation="MKFLDLFAGIGGFRLGMESAGHKCIGFCEIDKFARESYKAIHNT
                     KGEIELHDITAVSDESIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRRERTRRIFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFNQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNQGGEHDIAPTLTSNRYHENNHLSDGFRIRKLTPKECWRLQGFPDWAFDKAQEVN
                     SNSQLYKQAGNSVTVNVIAAIAKELS"
     gene            12431..12499
                     /locus_tag="VYN80_000507"
                     /pseudo
     CDS             12431..12499
                     /locus_tag="VYN80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            12496..12894
                     /locus_tag="VYN80_000508"
     CDS             12496..12894
                     /locus_tag="VYN80_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540464.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIISEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTYIGGMP
                     AVYLKGFGDGYAWTEYEEIIGNIYENPVRR"
     gene            12898..13323
                     /locus_tag="VYN80_000509"
     CDS             12898..13323
                     /locus_tag="VYN80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1540465.1"
                     /translation="MAIDIKKRLKALPYIDIKAKSKHQEIISLKSGILRGQQFDSMPK
                     SKSNKNQTEELNVLIIDKSEQLYKEIKQMYHERDELVQAIESLDDPVENIVMRLLYID
                     GLSWKEVQIKLNCSPATIQRAKHKALLKLSKMYDKNDSK"
     gene            13410..13976
                     /locus_tag="VYN80_000510"
     CDS             13410..13976
                     /locus_tag="VYN80_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1540466.1"
                     /translation="MVVVEPIRNRDDVQLMIEWLALHSAVKESDRQRNLMLFLSGVNL
                     GFRIGDIVKLKVKHVKGWHVQIVDEKTDKPTKRKMPKKFKNAMRQYIKDKKDEDFLFP
                     SRNGKHQHIKPNTAYKIIKRAAEEVGLENIATHSMRKTFGLFMYEQTKDVALIMDLLN
                     HSSQSISLRYIGKNQDSQDRAMTKFQGF"
     gene            14142..14348
                     /locus_tag="VYN80_000511"
     CDS             14142..14348
                     /locus_tag="VYN80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540467.1"
                     /translation="MKGIIKRLFNKRTTKQKPLGKIVVGVEIENRSELKELTQQCCEA
                     IEHLNNCIDKLNKFELKVSTSIIK"
     gene            14345..14641
                     /locus_tag="VYN80_000512"
     CDS             14345..14641
                     /locus_tag="VYN80_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1540468.1"
                     /translation="MIEVSTREERNQFYNSSEWRTIRRQALKRDHYECVWCRDEGKVT
                     TTNLEVDHIKELELYPEFALDIDNLRTLCKACHNKRHDRFDKNDRNFRKDEWWG"
     gene            14760..15098
                     /locus_tag="VYN80_000513"
     CDS             14760..15098
                     /locus_tag="VYN80_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540469.1"
                     /translation="MEEYSEKNIKELENQLLSKIGYFSPRKKDAIQYEKVNRYLYLVR
                     LLYELKAKLHEDGLVITVHNGQQRFQKANSLIKEINTTSNQLLAIERSFDFEVENSPV
                     EKPTSGSDLL"
     gene            15095..16735
                     /locus_tag="VYN80_000514"
     CDS             15095..16735
                     /locus_tag="VYN80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV1540470.1"
                     /translation="MISHPLVDDYIKMAESGEIVVNKERKLLFKIIKEKIYPRDDLYF
                     DNDLIDKFIRFAEKNFFPLAKYQLFLTPFIFLFRKEDGEPHFDEYLYTLARGGGKNGF
                     MSARSSFFISPIYPIRDYDVTITANSEKQGKVSFEEVYETIQRRGLEDHFYLTKMSIT
                     GRANNSVFSFRTNNPKTMDSARDGCLEFDEIHQFEDDKAVKVQRSGLGKIAHARTFYN
                     GTNGYVREGFYDKLIEKSMQILNGEVDDFRLFPFICKLDSADEVDDMKKWPKANPMLD
                     ESTPYAKRLLARTKADYDDLELEPSGRQEFMTKRMNLPEADLEKDVTSREKLVACLRS
                     PGIDLLGKSCVAGFDYASIRDFASVGLLFKNGDEFIWKQHSFARKVFLKAFKLKAPIQ
                     EWADKGLFTIVDGPSIDPRLLVAKLEEWSKFYQIELVCADGFRMDLLKPLLEEAGFEY
                     EFLRNPGAIQSKVAPIIEDGFANERFVFENDNSMIWYTDNTYIKEDKDGNKRFLKKEP
                     VRRKTDGFHALIAALYKRELVHESNVGEFLDMLDSWDF"
     gene            16792..17940
                     /locus_tag="VYN80_000515"
     CDS             16792..17940
                     /locus_tag="VYN80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1540471.1"
                     /translation="MGFLNLFKRDAPDVGFDFEELERMLGRLQLKSLAIDKSAEFVAR
                     IFAKSEFRYMVENHHVTSDWDYILNVRPNKNESASEFWQKVIYRLLTKNEVLIVLSDD
                     KQLLVADAFTRRRYALYDDTFEMVSVGDYTFQRKFAMSDVIFLQYNNNRLQEYVSDLF
                     SDYEKLHTRLVEALARNNQIRGVLATKANGTFDEDKRKKLQRYADNLFKSFTSKTVAI
                     VPTMDGVEYSELTNTVGTTNLSVEELKKLRRQFDDEVADILGLPTALMHGDMADLSNS
                     QKMFNSYCYESLVKKVGDALNHAMVSSAGYANQKKFVIFGEGGLDKFALAENIDKLIS
                     SGAMTRNEVRLELGLEAVPGGDSFLITKNYQGENEIEKGGNEDDNHSD"
     gene            17921..18643
                     /locus_tag="VYN80_000516"
     CDS             17921..18643
                     /locus_tag="VYN80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEV1540472.1"
                     /translation="MTIIQIKGAIVSNDDRWFYDWIDMDATAPKDIVLPTTGEDVEVH
                     INSGGGDVYAGSEIYTALRAYQGHVTVKIVGIAASAASVIAMAGDQVEISPTAQIMIH
                     NVSAGVRGDHKALLHEAGVLEGFNKSIAYAYIDKTGKALDDLLDLMEGETWFDARSAV
                     NHGFADKVMFESQEAPVLVASQTPVIPRDFIEKIRSAMTPDVELLADLVRDRLNNSLL
                     IPEDKKKEDKTAEPVGLGRFPF"
     gene            18662..19777
                     /locus_tag="VYN80_000517"
     CDS             18662..19777
                     /locus_tag="VYN80_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1540473.1"
                     /translation="MMKLGNEFTTARQNFMNAVETGAPMEEQNKLYNEMIEALTDKLQ
                     DDAREAARKEIATMNPYDAQLTAEAREFFNNIEKLPPKGIEKLFPQETIDRIFEDMVA
                     ARPFLQHIGLRNAGIRLKFLKSSRTGQAVWGKINAEIQGQLKQEFAEEEAIQSKLTAF
                     VVIPKDSEKFGPAWLQSYVSIQITEAFAAALEAGYLNGDGDKKPIGLSRTLTGTAESE
                     KVTYAEKQAQKKPLTFADSATVVKELTEVYKYHSTKADNTTPVAVEGNLVMVVNPVDA
                     WDVKKQYTSLNAQGVYVTAMPYNLILVESVAQTSGKVTTFVKGRYDAFVGGGIEFGRF
                     TETYALEDLNLYTAKQFAYGKAHDEKTAAVWTLNIGK"
     gene            19791..20081
                     /locus_tag="VYN80_000518"
     CDS             19791..20081
                     /locus_tag="VYN80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEV1540474.1"
                     /translation="MEIEAKLHSLLDPFKERMRIFHSGEDANLSRMLESSQMVITRLV
                     GSGNIHDHQIRELILERARYVYNDQVEFFYENFKADILALSLDKIEMEDSDD"
     gene            20074..20262
                     /locus_tag="VYN80_000519"
     CDS             20074..20262
                     /locus_tag="VYN80_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540475.1"
                     /translation="MIKVLKEFYDLKEGVLRQVGERFEATEERFAELKEHLPGYVELE
                     KSSRSRSSKEVVSVNEEE"
     gene            20249..20638
                     /locus_tag="VYN80_000520"
     CDS             20249..20638
                     /locus_tag="VYN80_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1540476.1"
                     /translation="MKRSEGPSYRYKKPEYQNGDLRTPLTFYTSKIKKGVDGRDVSYE
                     KAFYTMGQTYSPSMKDVEIATGKAMKAKMTLKIRDPLTDYQPENRHFVEVGDIRLVGK
                     KWQIIDVRPDYDNRDFLIVLIGGGRDV"
     gene            20631..21029
                     /locus_tag="VYN80_000521"
     CDS             20631..21029
                     /locus_tag="VYN80_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540477.1"
                     /translation="MSSGVNLKGFDDILRNIEARLGEPVVRRNVNRALKETVEEFEPT
                     FKRAMAVYADTGKTVGAVVYGNVTGTSSGVPMVKLGFKSPRWTLIHLNEFGYAKNGHP
                     RGFGVMRRFFEASKPVFKSKVGMKLKQEFL"
     gene            21029..21409
                     /locus_tag="VYN80_000522"
     CDS             21029..21409
                     /locus_tag="VYN80_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540478.1"
                     /translation="MIKDKLTELYDALEEDESLSGISIKSFERPESLGDDETSIVIIP
                     VGPPMQTAHGSNTSLAKTFLYQINVESTNRVECKELQGRIEKIMEEQGFYQTEGGLEQ
                     WIPDIKRYVDVRTYRGMSALYEKY"
     gene            21426..22022
                     /locus_tag="VYN80_000523"
     CDS             21426..22022
                     /locus_tag="VYN80_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1540479.1"
                     /translation="MTVKGTALIGLKSVTIRVHDGQTPTVGKNLFTLEGKDNEGATQT
                     ARVTGLSSDPVKTYGSNVAYHVSNRGVGDVKVEMGLLDVPLALYTNALGYGDDDGIYY
                     FGADTVAKNVSILIESNTADGEPVYYGFYKGQLSMDAIDFETIKDKANELATTNVNFA
                     ATASSDTATKGRYGAIVYGSDAEKLKKLKGQLNMAAAG"
     gene            22071..22364
                     /locus_tag="VYN80_000524"
     CDS             22071..22364
                     /locus_tag="VYN80_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540480.1"
                     /translation="MAKVQFTIKNDKGEDVLKKSKEITTRDYRDYLVMNDSLTSDLSE
                     VEKLDKQLGFIASLFDDVTVEQLLEYTDFAKVISIFTDIYAHLVGDVDPKEKS"
     gene            22613..22843
                     /locus_tag="VYN80_000525"
     CDS             22613..22843
                     /locus_tag="VYN80_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540481.1"
                     /translation="MSRKEKARRRYWFWFTIFILYLAFGLYCICTNFGDTLGMLLLSP
                     FIFASLPLYAYLFLGLFIWAFMSLAMDDFYKE"
     gene            22900..27075
                     /locus_tag="VYN80_000526"
     CDS             22900..27075
                     /locus_tag="VYN80_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1540482.1"
                     /translation="MASGTPLGAMYIELGLDVSNFNPTLTGAKNAVKYFQNTVRSLDS
                     TLKGNEKNASLLQAKYKTLGQAIDSQRKVLDEMKKSFDKLEPGTAKFDKAAADIQREN
                     AKLAAMEGQLRGVEKALQDVGRENSWSGKMDALSEKFAKGGKKLRAMGDAMKPVSAAL
                     LAGFTLSTKKAIDFESQMNTTKSLLADTIPTADELNSTTQKLGESSKNWAKQYGISTA
                     SINEGMQEIIKKGFDANQTIEAMPAILDAAKASGEDFNTVMNATTNILQQFGLSTQDT
                     ERVTNSLTFVANKTAAGFADMGAAMEYVGPVAKNVGMDLEETAATVGLLSNNGIAGEK
                     AGTALRGALTRLLKPSKQNANAMAKLGFSAEEFRSGAIKLPDILDRIKKNTEGMTDAQ
                     KSALIATAFGTEAQTAMNILVDQGGDALRNLANETKGATTYTKDLANELSKSSKNGVE
                     RFKSSLEVLQINIGQKLLPLLTPVIEKVNQFIDWLSKAPPAVQSLAVGIGGFLALGYP
                     LLNFLGNGATALSILIKHAGKLGGLLKGGIGLAKLTERAGKLKGLLQGGIGLAKVGSE
                     ATVLAEGAGVAASSSGLLSGAIGALTNPIGLLVGGVALLAGGLVYLGHKKDEARIKAE
                     EFGSQLSDTARKELRDFQSKVDETSKAVANFGTHAGDAEKVSGAFKKLYDEIAAGAEK
                     SNQRMQELTGKWGLSEEDLAKAREKNAQMVSNAESMMNKINDIYQRHNGDASKFSQEE
                     KEIILNNQREMIKTRISMMNLSAEQQKAALQALNGEIGSLNETQLKHTRDVLKKSLDE
                     EKQLYETSKSELKELLEGKVIDQETYNKKLQTLETNHQQTMEALGTKYYQVMQNLDAK
                     VKARTGQSWNYWEEAKKVLEEYGLSYEVIGQKATEASQKMGESNSILAKYTSDMSKEI
                     REANDAWSLLVGNINENGNFEIKSNVKEVIGEATKSAEGWEQLQFIVKNANINSNARA
                     TIAETLVESGKWSTMTLEEKQLIVQNQAGLQAIFDSENHLRIWNSMPAEVKQLLLENT
                     DVMNKAEEASKALYNYDALTPKQKELLATDENFRKAVARSTDTLTTWNATTPFTKDLK
                     LDPTNVLNNGQLSIDKIMAWNLSNPETKSLNATDNTSEAVDSAKASVNSPKQEKPIDL
                     LAKDQTAGVRNETSAAINAIKQNNPVDILAKNSTSSTVSEVKSGVNSIQDKTVTISAR
                     DNASGVLAGIRSWITRVTGDFFTNVFASRHAHGTNYHPGGLAMVNDQRNSTYKELVTL
                     PDGRSFIPEGRDVLLPLPKGAKVLRADKTKRLMREMGVPKYVAGVGIPSDAKFLREME
                     EAQAKIVVQAKGETNSKDSDKVVSEIAILRASMEKILTAILEKPSDTYLDADKISMSV
                     YQRQGAIYAREGM"
     gene            27075..27773
                     /locus_tag="VYN80_000527"
     CDS             27075..27773
                     /locus_tag="VYN80_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1540483.1"
                     /translation="MFYMIINGFNTSTIPHCVVTDFGQVEAAKPRVVEDANLYGANGS
                     YRVLDGGYESYERTFSFYIPKLLDVSTIVEKFQPKDNVLEFSYQLGSVFYADFIGATY
                     SPHGMHAWKLEIKLNMQPFRYQKNVAPLIFAASGNINNPGSVYSEPVIEIEGDGDISL
                     TIGRTTMHLTVRRKVTIDCRHKKQNIYNADGAVQNTLRKRGGFFELAVGNNSLVFTGS
                     VRKVTVRPNWRYIL"
     gene            27770..29387
                     /locus_tag="VYN80_000528"
                     /pseudo
     CDS             27770..29387
                     /locus_tag="VYN80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            33992..35913
                     /locus_tag="VYN80_000529"
                     /pseudo
     CDS             33992..35913
                     /locus_tag="VYN80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841354.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            35894..36097
                     /locus_tag="VYN80_000530"
     CDS             35894..36097
                     /locus_tag="VYN80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540484.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            36100..36450
                     /locus_tag="VYN80_000531"
     CDS             36100..36450
                     /locus_tag="VYN80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540485.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            36459..36758
                     /locus_tag="VYN80_000532"
     CDS             36459..36758
                     /locus_tag="VYN80_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540486.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            36762..37094
                     /locus_tag="VYN80_000533"
     CDS             36762..37094
                     /locus_tag="VYN80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1540487.1"
                     /translation="MQQITEIIIAFATSFLTVAVGGIVKAVKDYLLRKGGEKAVIIAE
                     ILAKNAVHAVEQVASETGYKGEEKLEQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            37103..38059
                     /gene="lytA"
                     /locus_tag="VYN80_000534"
     CDS             37103..38059
                     /gene="lytA"
                     /locus_tag="VYN80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1540488.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHWR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYWFDNSGEMATGWKKIADKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTMADKPEFTVEPDGLITVK"
     gene            complement(38197..38376)
                     /locus_tag="VYN80_000535"
     CDS             complement(38197..38376)
                     /locus_tag="VYN80_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540489.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(38518..38667)
                     /locus_tag="VYN80_000536"
     CDS             complement(38518..38667)
                     /locus_tag="VYN80_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540490.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKA
                     RQRKR"
BASE COUNT        12276 a         6587 c         9107 g        10787 t
ORIGIN      
        1 aaaaaaaaat agaccttgtc cagaggtcgg ggagttggag ggggcaccct ccaaaagcat
       61 tgatttaata aggttttatt ttaccttttt cataataatc tcccttaact ccaccgaatc
      121 aggtggagtt ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg
      181 atatttttcg gtatttttcg gattttggtc ggggaattgg cggggacttt ttgagatttt
      241 ggtggggatt tttttagcga atatgactaa gaaataggtc tgttgtcgct tcagcaagtt
      301 cgtcctcaac ttggttataa cgatccgtca tataaacctt tgtatgcccc agcgcttggc
      361 ttaattgttc aagcggaacc cctgcaataa tgctttgagt cgtgaagaag tggcgcatca
      421 tgtgaggtgt tacatgcaat cctgttgctt cattcactag attgaagttt ctatttaact
      481 ggtttggatt gatgagacca cctttctcgt tgatagttat ataatccttg tgctgttcct
      541 tgataatccc taactttcgc ttaatcttag aagcttcagc tatcagataa tagataaggt
      601 ctgttccgat atcatcaagg cagacatatc gctctgaatc cttcgtttta agcccgcctt
      661 tccctttcaa ggtctggttg cttcggctgt ctctaagatg cagtatagcc cgtccgctgt
      721 cgttctgagt gatgtccatt ggacgcaaac caaagacttc tcctcttctc aatccaaaaa
      781 tggtaagata ggtcagagcg tagaattgtt ttggcataat ctcttctgcc tttgctatcc
      841 aagtcttgaa ctctttgaga gtcactttct tgtttgcagc agggatatca ctctggccga
      901 tgaaaacacc tttcaagcga tttgagagca gatttccatt tttgacggca tcattcagca
      961 atgccatgaa gctggaattg agggtttgaa cagtgtatct ggtatggttc tgcaactttt
     1021 cagcgataaa gagttcatac tcatttctat ccaaattttt aagcagggta gaaccaaact
     1081 ttgccttgat atggttcttg tagagattat cattgaggta gtaggaagtg tcattccagc
     1141 gccctgttga caatctcttt tcagaataaa tatcccaata ttgatcaagt gtcagattcg
     1201 tattgatacc taattcttgt tcttggattt gttgctcaag ctctactaag gctgcacgag
     1261 cttgtggaag ggttgtgaga ccacttttac ttttttctct ttttttacct cggaagaaaa
     1321 aagaacgtct gacatagtat cgcttgcctt tggcagtctc atagtaaaag atatttgggt
     1381 attttgtttt attatatttc attgtattct ccttgtttat cggcttctgg acaaggtcta
     1441 aacattgaga atattgacat cacccctttc atggtgtaaa atagggtata gaaaagaggc
     1501 ctttttaatg gctgattttt tataagggta agcttcacaa tcaaactttg gcgagggaga
     1561 ttgtggagct tttttgttaa ttactcctca agcaatctta taaaatcatt ttctgtcatg
     1621 atttcaatat cttgaccttt ttctaacaag gtttgtgctt ttttcatttt actacttaac
     1681 ccatctgtac cgactactct ccaatcttgt tctcctacaa ctaagatatt agtgtgttta
     1741 gtcactcctt tttcaggaat accaccaact aatgcagtag ctttgttagc ttcttttcta
     1801 gtcattcgct cgagttttcc agtaaagcaa aagtacaaac cgtaaaagta atggtctggg
     1861 ttcattgctg ccttttcttc ttctgttggc tgatagataa gattttcttt gtactgataa
     1921 ctttttttcc ttttaaatcc atactgacca agtaagccag ttttattgta gctatattct
     1981 tttaaaaaag cgttgaggtc ggaaaatgaa ttagtagata ataagtattc taaaatcaat
     2041 ccgcttgctc gtgcatctga taaagcgttg tggtggtcta gctcaatatt caaattttta
     2101 gctagtcttt ttagtttgta attcaattgt ccagggagag cgaccttggc taatcgatac
     2161 gaacaaatat attctatatt atcaaaatcc agctcgtatt tttggtatac atctttaaga
     2221 gcacccatat caaactgtgc aaagtgggct acaactatat cagaaccaat aaaatcaaca
     2281 atcgcctttc tcacctctgg gaatgtaggt gaatcaagaa catcttcagg tgtaatgcca
     2341 tggatgaaaa tattgaaatc atcaaactct tcttctggat tgattaaagt gtaaaaagca
     2401 tcaacaatat ttccatcttt aaattttact aatccgatag aacaaacact accgcgaaag
     2461 tcattcgcag tttcaacatc taaagcaaca tacgagtaag acatatgagt ctcctttcac
     2521 tccatcaatg caaagtattc ctcttttacc atgacttcat tcgtcatggt ttttagattg
     2581 tagtaggaca tgaatttgag gtaatcaaac tctgtggggt cgtctaagct ttctatcgcg
     2641 tcttttacga gatgatggat catattccta tcagcttcgt tttcacagcg taggcgagcg
     2701 ttctggtact ctgagcgtgt gtggtctttg tgtccaagtt catgcagtag tactttaact
     2761 ctctctttct tgctgagttt gttagacaag aaagctgtat tggtttcttt ttcgtaaaat
     2821 ccaagttcat caggcatcaa atctccatca aaatcgataa tacgaatctg aaaatgactt
     2881 ataatttctt tttcagtcac taagcagtac ctctaatcac cagcttcttt gagataacct
     2941 tcaatgatag actggatgat tttcttcttt tcatctgtta attctcgacc gccaaacatc
     3001 atgacattag atgccatttc ttcaacgttt agagtcttac cttcccattt gtagtcatga
     3061 ctaccagcga ttgttgggtt atccgtgcga ccaagtaaat aatctgtgga cacgttgaag
     3121 tagtcagcaa tcattgaaac tcgttcaaca tttggtgtgg atttcttcat gttataaatt
     3181 gtatttctgc taaaacctag tttttcttca agtttattta atgaaatacc ttgtttgtca
     3241 gccaattctt ttattttttc aaatgtgaaa aacattgata catcaacctt tctaaggcat
     3301 gacaaaaaat atttaataaa tttactacaa aactgttgac aaagtttaat aaatttacta
     3361 caatagtttt tgtaagctaa agagttagcg aacaagacaa ctaaaaaata aagcctaatg
     3421 aaactgattg gcgtccgttt tctaggtata accttacttt ttagtaggtc ttttctctat
     3481 gttttgattt taataaattt atttatcaat gtcaagaaat tcgctaactt tttagataat
     3541 tttttaaaag gaggtgagga aatgagacca agacgatatc cgtatagtgg gaaaaaagag
     3601 tctaccctcg taaaggcaga ccctgaatta gtagaaaaaa ttctaagaaa cactagtttt
     3661 cttgagcgtt tacaagttct gttagcaacg aaaccgtagt ttctgtactg agtttatgag
     3721 cgacaaggac gctattaaga atagcctttc taatttccat gtcgattggg tattctttaa
     3781 atacctcatc taacatatct tggataactg gaatagcatc gttgataatt tcttgagaaa
     3841 cttgtaaaac atcttcttta gtgagttttg acatataaaa tttcctcctt tctattggaa
     3901 ttttgactaa aacggtgaga ggtcctagtc aagattatta tagcaattta ggaagatatt
     3961 acctcagtct tgagactgat ataggaggtt gaatggaaga taaaatcatc gaacttgctg
     4021 attacttcat cagcgagaac acaacgtaca gagaagctaa aatagcgtgt gagaagctat
     4081 ttaaacaagc cagccatgag atagaactca gagcgctgga aagtgaaacg aagaaataga
     4141 aaggagaaag atgagtaaag aactaaagat aatcaaggct aaaatcaaaa ctcgtttgat
     4201 tgagttggat atgactcaag ctgaattggc aaaacaagta tctgtagcat catcagttat
     4261 ttcagagttg ctgaagtatg gcaaaggtag tgagtctgtt aaggaaaaag ttgcagatgt
     4321 tttgggtatt gaaaaccctt ggagaaatca ctgagaggtc catacatgca agcgaaaata
     4381 atactgaatt ggcagaagaa aaatcaccaa cttagtcaga tgatgatcga tagtcttgag
     4441 ggactagatg tttgggaaac tattttaaca ctaggaaaag taagaagagg aatattatga
     4501 acgaaatttt taattttcac gggcaggaag tccgtacttt gacaattgat gacgagcctt
     4561 ggttcgttgg gaaagatgtt gcagacatcc taggatatag caaggctaga aatgcgattg
     4621 ctcttcatgt tgatgaagat gacgccctaa aacagggcct cacagataat ttaggaaggg
     4681 tccaagaaac tattatcatc aacgaatctg gtctctactc tcttatctta tccagcaagt
     4741 tgcctcaggc taaagagttt aagcgctggg tgacatcaga ggttttgcca gctattcgca
     4801 agcagggcgg tttcattcgc gaggacttgg acgaggatgc ctttatcgct ctattcactg
     4861 gtcaaaagaa attgcgtgag caacaggtta ccatgcttga agatattgac tacctcaaga
     4921 gtgagcaacc gattcatcct agctatgctc aatcattact gaagaagcgc aaagctcgtg
     4981 tcgtggcttg cttaggtggt attgatagtc cagcttatgc ggataagact ttcgctcagt
     5041 cagtctttag acaagctgag attgatttca aagaccactt caacattagt cgctatgatt
     5101 tgctaccgaa aaagtttgca gatgcagcct tggcctactg gatgacgtgg gagccaagca
     5161 caaacactaa gatgaaaatc atgaaattaa actcatttga tgacgtctag aaaggggaag
     5221 aagatggaca atgttctact ttcactgtct gaatggatta aatctattat caaggacaca
     5281 atcacaaggc tagtcgaaat agagaaagat agtgatcact atccagagtt gatggatgtg
     5341 aacactacct gcgaatttct aggaattaag tatgccacct tttcagataa ttatcgttac
     5401 ttaaagggat ttccaaagga attacctggt aagaaatggt caaaaagagc catcaaagaa
     5461 tggctctcta atcaaatata ataactttac taaaaggctt ctggacaagg tcttagcaaa
     5521 attatttgac tatattatag cacaaaaaga ggataaggag ataaaaatgt ttgaaccacc
     5581 gattttagac cagttgatgg gggttggagc cttactgctt ggatttgcag gggcttgccg
     5641 tcatatcaaa ttacaggaac aacgcaagga agaagaaaga cgagaagagc aagaatttgc
     5701 gtctatgatt attcaaggct ataaccatgc ttacgaacgt ggtagagaag ctgaacgtca
     5761 agaaatccgc aagaatattc gtcgtccgtt caagggcttt acctacgaca atgaaccgcc
     5821 tcaaggattg cgtcctgagc cgttagcttt gccagaacct aaacagtctg caatcagatt
     5881 tttgtaatga ggaggtcagg aaatggaaga attgattgaa tggcttgaaa ccaaagaaaa
     5941 attaaacagg gaaatgattt ctgacgagga aacatcggac tttaacctac atctagaaaa
     6001 tgataatgat accatgttca tgatcaagga atacttgaac gattataaca agctagccaa
     6061 agactatcga attctatcgt ctgaaaatcg tctacttaag cttgaaaaaa tggagctgga
     6121 aggcaggcac atctatgagg atatgcggat gaagtaccgt gccaatcgta ggaagtgggg
     6181 tgctcggtat gtctgaaatt aagtggataa aaatcacaac cgatattttt gacgatgaaa
     6241 agatttgcct gattgatgcc ttgcctgatc ctgatgccat cttagtgata tggttcaaga
     6301 ttttgacact tgctggaaaa cataacagta atggtttgtt gatgatgact gataaggttc
     6361 actatacaga tgaaatgtta gctactattt ttcgtagacc attgaataca gtaagaatgg
     6421 ctattggagt ttttgaacag tttgggatga ttgagattat cgatggtatt attagcttgc
     6481 caaattggga aaaacatcaa aacgttgacg gaatggagaa aatcaaggaa cagacacgta
     6541 accgtgtagc caaataccgt aaaaaacaga aaaatcttgc tcttggtaac gttacaggta
     6601 acgttacagt aacggacggt aacgcactag aagaagataa agataagaat aagaatagat
     6661 tagataaaga taagaataag aaaagaataa ctactactag tagtggtagt gaagaaaata
     6721 tcttagaact ttttcaatct gagtttcgta gactcttatc tggatttgaa attgaagaaa
     6781 tcaaccatct actaaatgag aatgatgtgg atttggtgaa agaagcattg aagactgcta
     6841 ttaactcagg aaagccgaac atcaaatata ttggtgggat tttaagaaat tggcagatga
     6901 acaatgttac cactgttgaa caggttcgtc aatcggaaaa gaagaacaag gataagaaag
     6961 aagaacagga ggccaaggac gaatgggggt actagaacta attgaacaat tcgagattga
     7021 ctattatccg ttaagctacg agaagaaaac tcttttagca gaccaaccaa ttcatcaagt
     7081 ggttgcctgc ttgtctgaaa tggctagctg gcatgaatgc ggaggtcggc tagtatggta
     7141 gacaatgtgt ttgaggaaat cgccttatct tatcgtagga atacagaaca acaagaagag
     7201 ttctgcgaaa agcataacat ccctttgata aagatattga ggaccgagag tgttgtatgt
     7261 cgcatgtgtg aatctgagcg gattcatgaa gaaaatcagg aaagagtgaa tgaactggct
     7321 aatgctgaga atgagcgaga gaggaaatac tatcttgaaa agttctctct ttatgatgaa
     7381 gttttgaaaa atgctacttt ggacaatttt gaaacaccaa ccgaaaaaga agcggaaaag
     7441 ctagcttttg caaagaggat ttgtcgtgag tggtctgagg gtgctaggaa caacatcgtg
     7501 ttacaaggag aagctggaac aggtaagagc catttggctt ttgctatggt taaggctcta
     7561 tctgagtaca cgaaagagat tgctattttt attaacgtaa ctgacttgct gatgaagatt
     7621 aaagctgatt ttagtcagga agagtttctg gtcaataaaa ttgctagtgc taagttcttg
     7681 gttttggatg atttaggaat ggaaaaagat agcgaatggt catttactat tctctacaat
     7741 atcttgaata agcgttcaaa cacaatcatt accacaaatt tgatttctgc cgatattcaa
     7801 aaaagatatg gcagaccttt catgtccaga ctaatgaagg gtgtagataa agaccatttg
     7861 atggttttca atgatttgac aaacaagcga aagcaatatt tttagaatag gggtggctga
     7921 tgtttatttt aaagcatggg acaagagagg ataagccgtt tctaaggtct gcaattatca
     7981 gtgtgactgg attggacatt tcatgttctg aggagaagaa agccatgcgg tttgtttctc
     8041 gggcggctgc cgtacaggtt ggaaaggctt tgaggggttc ctttgggaac ttttaccctg
     8101 ttgaggtgga gtgatgatga aattcttaaa tggtgactgc atggacatca tgaaacaata
     8161 tcctgataat tactttgacc tagctattgt tgatccacca tatttttctg gtccagaaaa
     8221 aaggaaattt tacggacgga aaatcagtcc aataggtgta agcagactct atggcgaaat
     8281 ctcagagtgg aaaattccaa acagagatta ttttgatgaa cttttcaggg tatctaaaaa
     8341 tcaaattatt tggggtgtga actacttcga ctattctttt ggttctggcc gtattgtgtg
     8401 ggacaaagtt aatggtcaat caagtttttc agattgtgag atagcgtact gcagttatca
     8461 tgatagtact aggctatttc gttacatgtg gaatggtatg atgcaaggta aatcaatctc
     8521 tgaaggtcat atacaacaag gaaataaggc attgaatgag gttagaatcc atccgacaca
     8581 aaaaccagtc aacctttacc tttggttatt gcaaacttac gcaaaagaag gcgataaaat
     8641 acttgatact cacgtcggtt cagcaagtag cttgattgct tgcggagagc taggatttga
     8701 ctatgttgga tgcgagattg ataaaagtat tttcaacctc gctcaacaga gacttgatgc
     8761 ttatgagaag cagttgaagt tattttagga ggtattgatg ttagagcttt acttcgtcta
     8821 taacgggcac tgcaagtttt accttgggag gtttgacaat gtcgatgatc tcattgagca
     8881 gatggaagat catcagtggg ctttctcggc tatcactcat ccaagatttc agaagcacat
     8941 tggtcagcgg acgacacgat ttgactacgg ttctaaggat tgttactatt tagcgacttt
     9001 ttcaggagga gaaaaaaatg attgaactta ttaaagaatt tggaatggct attctgtggt
     9061 tatttctcgg ctatttagtc ggggaacgtg cagcaagaaa ggaaaagaaa gatgatcaat
     9121 aacgttacat ttttagtggg aggtaagaaa catggttgga gtaacctatc aggaaattca
     9181 tctctttgtt gaatttttga aagagcagta cgggcaaggt cgtccagact atattgaagc
     9241 cctgaacgac ttagacggtc tggtggaagt ctcctacaga gaagctattg aaagattttt
     9301 agaagatgaa gtacgataaa caggctgaga ttgacggact gaaacgcaca atcgagcaaa
     9361 acgaagagaa gataatcgag tattcgaagc cgtgtgatgc acgcaagaga cggattagag
     9421 cgctggagcg cgatttgttg aagaaaaaga ataaagaatt aagacggaaa gcggaggagt
     9481 tggaagatga tggaagagtt aaagaaaaaa gttaatgcag tatacaactg gacggtagaa
     9541 gacgggaagc cgcaacctcc ccagcaagat ttaccacaag cagtgaaaga ccgggcggac
     9601 tatttttggg aaatggcaga agatggtatg acgtttatgg gagcgatgga atgcatcttc
     9661 gctgatgaaa agcctacaga ctatgatttg ggagctacta agggttggtt gccaaaatct
     9721 aaggagtttg atgattgggt tggctattcg ccaggaatgt ctcaggtagt tattgcagtt
     9781 tatttgattt atggaggaaa ctaagatgaa taagcaggaa ttgattgaga aatataaaaa
     9841 acttgagggt gtatggaatg ctgaaggagc agaactagct cgtcaaattt ttctgcaaga
     9901 cttggaacaa ctagataaac caaaaccagt caaagttccg cagtgtgtgg cggaatatat
     9961 agaatttaaa aagaaaaaca attttcatgt ttacggtgca atgagagtaa ttgaagatca
    10021 ttatgataag aaagttcctg agtggtttta cgaaaataac atcgaaaaat tctgtcttgc
    10081 ttggcttgac ggctacgagg ttgaaaaaga gaagcggtat tttgttaaga ttaaagggaa
    10141 tattaaagaa aatatgttgg tttatggaga acttttgaaa aggtatttct ttacaaaaag
    10201 ctttagttta gacgatgtta tatattccca cacccgtaaa gaactagaag acgcaaactt
    10261 cggctgggtg tttgattgtg aaggaattga tatagaggag gtggaaaatg aataaagatc
    10321 taattgaaac accacgcttt aactttttta taggggatga agttctcttg aaagggaaaa
    10381 tagtcggttt tgatgtagat gagaacaagt gcgttgaaaa tgttgttaga ttggaatacg
    10441 ggcaaactct caatgtaccc aacaataata tttatattac agacgacatc gttgataaat
    10501 ccaaaattaa agtcgtagta ccgcagtttg tggcggattg gtatgaagag aataaggatt
    10561 cttttgaatt taatgtttgt gattggattg ctttcaggga tgaggctaaa aaatcagaaa
    10621 atagagagtt taataattgg attaataata gcagagaaaa tcctattcaa accctcgtca
    10681 acatgaacca attcggctac gaggtcgagg aagagaagcg gtatttggtg actttaaaaa
    10741 ataggcagcc tttggtcaaa tcgcaatcag ggagtactct ttattttagt caagatataa
    10801 cagctaggaa ttataaaggt actcaaaaag aactagaaga cgcaaacttc ggctgggtgt
    10861 ttgattgtcc agggattgag attgaggagg tggagtgatg agccttacgc taaatagcac
    10921 aattggagac ttagttttgg caatcggaga aattatcgtt ggttctgatg gtaaaaccac
    10981 tacagcgata ctggagatac ctgatcaaag cttttactta gagattgagc ttaaattgaa
    11041 ggaggaggtc ataaattgaa acgattcata gctatctgga ttctgctatc tgctggattg
    11101 aatatctggc agagtatcta cattaaaaag ttagaagaaa agcgccctat tgtcgtctat
    11161 aaagctgata acgcaggcgc tgagatattc ggtaaagtcg tcgagaaagg acggcatggc
    11221 aagctataca cgcttaccat tcgtgactac ggggtgttcg tggttacgaa ggacgtgtat
    11281 gagaaagtga aagttgggga tgaggtgatg ttgtgaaatt cttggattta ttcgcaggaa
    11341 ttggcggttt tcgtttagga atggagtctg ccggccataa atgtattggc ttttgtgaaa
    11401 ttgacaagtt tgctagagaa agctataaag cgatacacaa tacgaaagga gaaatagaat
    11461 tacatgacat cacagcagta tcagatgaat ctattcgagg aatcggaagt gtggacatta
    11521 tctgtggagg atttccgtgc caagctttct caattgcagg aaacagacga ggttttgaag
    11581 atacacgagg aactttgttc tttgaaattg ctaggttcgc atctattctc agacctaaat
    11641 atctattcct tgagaatgtt aaaggattgc tcaatcacga aaatggagtt acattcgaga
    11701 ccattatctc aaccttggat gaactggggt acaacgtgga atggcaagtg cttaacagca
    11761 agaatttcgg agtcccccaa aatcgggaac gagtgttcat tatcggacat cttagaagag
    11821 aacgtaccag aagaattttt cctctcagcg gaaaaaatca gtcaactagt aaccaatcag
    11881 tcatgaaaat tgggaatata aacccatctg gcaacggaat gaatggggaa gtctatcaag
    11941 ctgatggtct agctcccacg ctaacaacaa acaagggaga ggggcaaaag atagcaataa
    12001 aaagcaatac tataaaacaa tttggggtat tgcaacccaa ttttaatcaa tgtggagtgg
    12061 tttacgaaac agatggcatc gcaccaacta tcagagcata tcaaggcgga ggacttgagc
    12121 ctaaaattat tcaacgtggt catggttata atcaaggcgg agaacatgac atcgctccca
    12181 ctctgacaag taatagatat catgaaaata atcatttatc agatggcttt cgaatcagaa
    12241 agctaacacc taaagaatgc tggaggttac aaggttttcc tgattgggct tttgacaaag
    12301 cgcaagaggt caactctaac agtcaattat ataaacaagc aggaaatagc gtgacagtta
    12361 atgtcatagc agcaatagca aaggagttat catgaacgca ctagaaaaag tcgaacaatg
    12421 gtttatcgac cgtgaaatca aagaccgaaa aggtcgctgg attgatggct cgtttgtcaa
    12481 agaggaggat ttggcatgat accgaaattt agagggttat ccattgacga aaaaagcaaa
    12541 ggagaatggc aatacggaca tttaattgaa gatagaggaa gagcatttat tatcagcgaa
    12601 gtggtagaag ccaatgaaca atacattact ataggttctt ggtgtcctgt aaatccagca
    12661 acattaggac aatcaacagg actcagagac aagaacggca aggaggtatt catcggtgac
    12721 atcgttaaat gtacaagagg atgtctccat gaagtatatt tagaaaaaga atacggtggc
    12781 acatacatag gtggaatgcc tgctgtatac ctaaaaggat ttggagatgg atatgcgtgg
    12841 acggaatatg aggaaatcat cggcaacatc tacgaaaatc cggtcaggag gtaagatttg
    12901 gcaatagaca tcaaaaaaag attgaaggct ttgccttata tcgatatcaa agctaagtca
    12961 aagcaccaag aaatcatcag tttgaagtca ggtattttac gaggacagca gttcgacagc
    13021 atgccaaaat caaaaagtaa caagaatcaa actgaagaat tgaatgtgtt gattattgac
    13081 aagtcagaac agctatataa agaaatcaaa caaatgtacc acgaacgtga cgaactcgtt
    13141 caagcgattg agtcgctcga tgatccagtg gaaaacatcg tgatgcgatt attgtacatt
    13201 gacggattat cttggaaaga agttcaaata aaactaaatt gtagccctgc caccatccaa
    13261 cgagcaaaac ataaagcgtt actaaaatta tctaaaatgt atgataagaa tgatagcaaa
    13321 tgataatttt aatgtggtaa attagtatca tgaagaatag cagagaggaa acctctgctt
    13381 ttttttgcat taaaaggagg tgaggatatg tggtagttgt tgaaccaatc cgaaatagag
    13441 atgatgttca gcttatgatt gaatggctgg cgttgcatag tgcagtcaaa gagtcagata
    13501 gacaacgcaa cctcatgctc ttcctttctg gtgttaattt gggatttcgt attggtgata
    13561 tcgttaaact gaaagtaaag cacgttaaag gttggcatgt ccagatcgtc gatgaaaaga
    13621 cagacaagcc aaccaaacga aagatgccaa agaaattcaa gaatgctatg aggcagtaca
    13681 taaaagataa gaaagatgaa gacttcctct ttccaagccg aaacggaaag catcagcata
    13741 taaaacctaa cacagcttat aagattatca aaagagctgc tgaagaagtt ggtctggaaa
    13801 acatagctac tcactctatg agaaagacct ttggtttatt catgtacgaa caaaccaagg
    13861 atgtcgctct gataatggac ctactgaatc actcaagcca gagtatttca cttcggtata
    13921 tcggcaaaaa tcaagattca caagacagag ccatgacgaa atttcagggc ttttaatttt
    13981 tttattttaa catcaattca ttgttttaag gttatgatga tttcatttcg cacatgcaag
    14041 ataaacgctt gataaagctg agttaaaact catgtagcga gttcactaga atatgtaaaa
    14101 caaggaattg aaagagtaaa aacaaaggag attgcacagt tatgaaaggt attattaaaa
    14161 gactttttaa taaaagaacc actaaacaaa aaccattagg aaaaattgta gttggagtcg
    14221 aaattgaaaa tcgctcagaa ttaaaagagt taactcaaca atgttgtgaa gcaatcgaac
    14281 acttgaacaa ttgtattgac aagctaaata aatttgagct caaagtatca acatcaataa
    14341 tcaaatgatt gaagtttcaa ctcgagaaga gcgcaaccag ttttacaatt ccagcgagtg
    14401 gagaacgata agaaggcaag cacttaaacg agatcattac gaatgtgtat ggtgtaggga
    14461 tgaaggcaag gtcacgacta ctaatttaga agttgaccat atcaaggagc tagagttata
    14521 tccagagttc gcgcttgata tcgataacct acgaacattg tgcaaagcat gtcataataa
    14581 aaggcatgat cgttttgata agaatgacag aaatttccga aaagatgaat ggtggggtta
    14641 ggtgaacgaa tcttaaacac cccccggtca aaaaaatcag gaaatttcaa aggagccagt
    14701 aagcggtctg cactcgactg tccaaatttt taacaaaaaa ttaaaggggg tggggggtaa
    14761 tggaagaata ctcggaaaaa aatataaaag aattagaaaa tcagctactt tccaaaatcg
    14821 gctatttcag tccgagaaaa aaggatgcga tccagtacga aaaagtgaat cgttatcttt
    14881 atcttgtcag attgctatat gagctgaaag ctaaacttca tgaagacgga ttggtcatca
    14941 ctgttcacaa tgggcaacag agattccaaa aagcgaattc tctcatcaag gaaatcaaca
    15001 caacaagcaa tcagcttttg gctattgagc gatcgtttga ttttgaggtt gaaaattctc
    15061 ctgttgagaa acccacgtct ggaagtgacc tgctatgatt tctcatccct tggttgatga
    15121 ctacatcaaa atggccgaga gtggagaaat tgtcgtcaac aaggaaagga agttgctgtt
    15181 taaaatcatc aaggagaaaa tctatcctcg tgatgatttg tattttgata atgacttgat
    15241 tgataagttc attcgttttg cggaaaagaa ttttttccct ctggccaagt atcagctttt
    15301 cttgactccg tttatctttc tttttcggaa ggaagacggg gagccacact tcgacgagta
    15361 tttgtacaca cttgctcgtg ggggtggtaa gaatggtttt atgtctgcca gatcctcgtt
    15421 ctttatcagt cctatctacc ctatcagaga ttatgatgtg actatcactg ccaattctga
    15481 gaaacagggc aaggtttcat ttgaggaggt ctatgagact attcaaaggc gtggccttga
    15541 ggaccatttc tatctaacta aaatgtctat tacaggtcga gcgaacaact cggtcttttc
    15601 ttttcggacg aataatccga agaccatgga ctccgctcgt gatggctgtc ttgagtttga
    15661 tgagattcac cagtttgaag atgataaggc cgtgaaggtt caacggtcag gtctcggtaa
    15721 gattgctcat gctcggactt tctacaacgg tacgaatggg tatgtgcgtg agggatttta
    15781 tgacaagctg atagagaagt ctatgcaaat cttgaacgga gaggttgatg atttcaggct
    15841 ctttcctttc atctgtaagt tagacagtgc ggatgaagtg gatgatatga agaagtggcc
    15901 aaaggcaaat ccgatgttgg atgaaagcac tccttacgct aagaggttac ttgcaagaac
    15961 taaagctgac tatgatgacc ttgagttgga accatctggt cgtcaggagt ttatgactaa
    16021 acggatgaac cttcctgaag ctgaccttga gaaagatgtc acctctcgtg aaaagttagt
    16081 tgcttgtttg cgttcacctg gtattgactt gttaggtaag tcttgtgttg cagggtttga
    16141 ctatgctagt attcgagact ttgcaagtgt tggtttgttg tttaagaacg gggatgagtt
    16201 tatctggaaa caacattcat ttgctcgtaa agtgtttttg aaagctttca aactaaaagc
    16261 ccctattcag gaatgggcag ataaaggttt gtttacgatt gtggacggtc ctagcattga
    16321 tccgcggctg ttggttgcga agctggaaga atggagtaag ttttatcaga ttgaacttgt
    16381 atgtgctgat ggtttcagaa tggacttgtt aaaaccgctt ttggaagagg ctggttttga
    16441 atatgagttc ttgcggaatc ctggggcgat tcaatctaag gttgcgccaa tcattgaaga
    16501 cggatttgcc aatgagcggt ttgtctttga gaacgataac tctatgattt ggtatacgga
    16561 taatacctat atcaaagagg ataaggatgg caataagcgt ttcttgaaga aagagccggt
    16621 cagaagaaag acagatggtt tccatgcctt gattgctgct ctatacaaga gggagctagt
    16681 acatgagtca aacgttgggg aattccttga catgctcgat agctgggact tttaatctaa
    16741 gaataaattt tgggtgggtg gtcggcaaaa ataagaggaa ggaggggaag catgggattc
    16801 ttaaacttat ttaagcgtga tgcgcctgat gttggttttg actttgaaga gttggaacga
    16861 atgttgggac gcttgcaatt gaagagttta gcgattgata agtcggcgga gtttgtggct
    16921 cgtatctttg ctaagtcgga atttcggtat atggtggaga atcatcatgt gacgtcagac
    16981 tgggattata ttttaaatgt gcgtcccaat aagaatgagt cggcctcaga gttttggcag
    17041 aaggtcattt atcgcttatt gacgaaaaac gaagtactta ttgtcttgtc tgatgataag
    17101 cagttactgg tagctgatgc ttttactcgt aggcgttatg ctctgtatga tgacaccttt
    17161 gagatggtgt ctgtcgggga ttatacattt cagaggaagt ttgctatgag tgatgtcatc
    17221 tttcttcagt ataataacaa tcgcttgcaa gaatatgtat ctgacttgtt ttctgattat
    17281 gaaaaactac atactcgtct ggtggaagcc ttagctagaa ataatcagat ccgtggggtt
    17341 ctggctacaa aagcgaatgg aacttttgat gaggataaga gaaagaaact gcagagatat
    17401 gcggacaatc tttttaagtc ctttacgtct aagacggttg ccattgtacc gacaatggat
    17461 ggtgtggagt attcggagtt gaccaatacg gtaggaacta caaatctatc agttgaagag
    17521 ttgaagaaac tgagacgtca atttgatgac gaggttgcgg atatattagg tttgccgacg
    17581 gctttgatgc atggagatat ggctgatttg tctaatagcc agaagatgtt taacagttat
    17641 tgttatgaat cgctggttaa aaaagtgggt gacgctctaa accatgctat ggtgagttcg
    17701 gctggatacg ctaatcagaa aaaatttgtg atttttggtg aggggggact ggataagttt
    17761 gctctggctg agaacataga taaactcatc tcatctgggg ctatgactcg aaatgaggtg
    17821 agattggaac ttggtcttga ggctgttcct ggtggggatt ctttccttat cactaagaac
    17881 tatcagggtg aaaacgaaat agagaaagga ggaaatgagg atgacaatca ttcagattaa
    17941 gggagcgatt gtatccaatg atgaccgctg gttttatgat tggatagaca tggatgcaac
    18001 tgctcctaag gatattgtat tgccgacgac gggagaagat gtggaggtgc acatcaattc
    18061 tggtggtggt gatgtgtatg ctggtagtga aatctataca gcattgcgtg cttatcaggg
    18121 gcatgtgacg gtgaaaatcg tggggattgc tgctagtgct gcgagtgtta tcgctatggc
    18181 tggtgatcag gtggaaatta gtcctacagc gcaaatcatg atccataatg tatctgctgg
    18241 tgtacgtggt gaccataagg cgctcttgca tgaggctggt gtgttagaag ggtttaataa
    18301 gtccatcgca tatgcctata ttgataagac tggtaaggca ttagatgatt tactggatct
    18361 aatggagggt gaaacgtggt ttgatgctcg ttctgctgtt aatcatggct tcgcagataa
    18421 ggtcatgttt gaaagtcagg aagctcctgt cttggtagct agtcagacac ctgtgattcc
    18481 tcgtgatttt atcgaaaaaa tccgatctgc gatgacccca gatgtggagt tattggcgga
    18541 cttggttcgt gataggttga ataacagttt acttatccca gaagataaga aaaaagaaga
    18601 caagacggct gaacctgtgg ggttgggtcg tttcccattt taagaaagga aataaaaaac
    18661 aatgatgaaa cttggtaatg aatttacaac tgctcgtcag aactttatga atgcagtaga
    18721 aactggtgct cctatggagg agcaaaataa gctctataac gagatgattg aggcattgac
    18781 agataagttg caagatgatg ctagagaagc tgctcgtaaa gaaatcgcaa ctatgaatcc
    18841 atacgatgca cagttgactg ctgaagctcg cgaatttttc aacaacattg agaaacttcc
    18901 tcccaaagga attgagaaat tgttccctca ggaaacaatt gaccgtattt ttgaggatat
    18961 ggtggcagcc cgtccatttc ttcaacacat cggcttgcgt aatgctggta ttcgtttgaa
    19021 attcctcaag tctagtcgta caggtcaagc ggtttggggt aaaatcaatg ctgaaattca
    19081 aggacaattg aaacaagaat ttgctgaaga agaagcgatt cagtctaaat tgactgcttt
    19141 tgttgtaatc cctaaagact ctgagaaatt tggtccggct tggttgcaat cttatgtctc
    19201 tattcaaatt acagaggcct ttgctgctgc tcttgaagct ggttatttga atggtgatgg
    19261 ggacaagaaa cctattgggc tgtctcgtac tcttacaggg actgcagaaa gtgaaaaagt
    19321 aacttatgct gagaagcaag ctcagaaaaa accattgact ttcgcagact ctgctaccgt
    19381 ggtaaaagag ttgacggagg tttataaata ccattctaca aaagctgaca ataccactcc
    19441 tgttgcagta gaaggtaatt tggttatggt agtgaaccct gtagatgcct gggatgtgaa
    19501 gaagcaatat acgtctctca atgctcaggg tgtatatgta acagctatgc cttataacct
    19561 catcttggtt gagtctgttg ctcaaacttc tggtaaggtg acgacgtttg tcaaaggtcg
    19621 ctacgatgcc tttgtaggtg gtgggattga gtttggacga tttaccgaaa cctatgcttt
    19681 agaagacttg aatctctaca ctgctaagca atttgcttat ggtaaagcgc atgatgaaaa
    19741 gactgctgct gtgtggacac tgaatattgg aaaataaaag gtgactaagg atggagattg
    19801 aagctaaact tcattccctc cttgatcctt ttaaggagcg gatgagaatt tttcatagcg
    19861 gagaagatgc gaatctctca cggatgttag aaagttctca aatggttatt acccgcttgg
    19921 taggaagtgg gaatatacac gatcatcaaa taagggaact tatcttagaa cgtgctcgct
    19981 atgtctataa tgaccaagtt gaattttttt acgaaaattt caaagcggat attttagcat
    20041 tatcgttaga taagatagaa atggaggata gtgatgatta aggtattaaa agagttttat
    20101 gacctcaagg agggagtcct taggcaggtc ggagaaaggt ttgaagcgac agaagaacga
    20161 tttgctgagc taaaggaaca tttacctggc tatgtcgagt tggaaaagtc gtccagaagt
    20221 cgtagctcta aggaagtagt gagtgtaaat gaagaggagt gagggaccaa gctatcgtta
    20281 caaaaagcct gagtatcaaa atggagacct gagaactccc ctgactttct atacctctaa
    20341 aataaaaaaa ggggttgatg gtcgtgatgt gagttacgag aaggcttttt atacgatggg
    20401 gcaaacttat tctccaagta tgaaagacgt tgagattgcg actggaaaag cgatgaaagc
    20461 taagatgact ttgaagattc gtgaccctct gacagactat cagcctgaaa atcggcattt
    20521 tgtcgaagtg ggggatatcc gtctagttgg taagaaatgg caaatcattg atgtccgtcc
    20581 tgattatgat aatcgggatt ttttgattgt ccttatcgga ggtggtcgtg atgtctagtg
    20641 gagttaatct aaaaggattt gatgatattt tgaggaatat tgaggctcga ctaggagagc
    20701 cggtagttcg tagaaatgtc aacagagcct tgaaggagac ggttgaggag tttgagccaa
    20761 ctttcaaacg ggctatggcg gtgtacgctg acactgggaa gacggttggt gcggtcgttt
    20821 atggaaatgt gactggtaca tccagcggag ttccaatggt taaattaggt tttaaaagtc
    20881 ctcgttggac tcttattcac ttaaatgaat ttggctatgc aaagaatggg catcctcgtg
    20941 gtttcggagt catgcgtcgc ttttttgaag cgagtaagcc ggtctttaag tctaaagtcg
    21001 gcatgaagtt aaaacaggag tttttgtaat gattaaagac aaattaactg aactctacga
    21061 tgctttggaa gaggatgagt ctttatctgg tattagtatc aagtcttttg agcgtcctga
    21121 gagcttggga gatgacgaga ccagtattgt cattatccct gtagggcctc caatgcagac
    21181 ggctcatggt agtaatacta gtttggctaa gacttttctc tatcaaatca atgtagagtc
    21241 tactaatcga gtggagtgta aagaactcca aggaagaatt gaaaaaataa tggaagaaca
    21301 gggattttat cagactgaag gtggtttgga acaatggatt cctgatatta agcgctatgt
    21361 agacgttcgg acttatcgtg gtatgagcgc tctatatgag aaatactaga aagaaggaaa
    21421 aagaaatgac agtaaaagga actgcactta tcggacttaa atcagtcacc attcgtgtgc
    21481 atgatggaca gactccaacg gttggaaaga acctctttac gctagaaggt aaagataatg
    21541 aaggggctac acagaccgct agagtaactg gattatctag tgaccctgta aagacttatg
    21601 gtagtaatgt tgcttatcac gtctccaatc gaggagtcgg ggatgttaag gtagagatgg
    21661 gcttgcttga tgtcccatta gctttatata cgaatgcttt agggtatggc gatgatgatg
    21721 gtatctatta cttcggtgcg gacactgttg ctaagaatgt ttccattctc attgaaagta
    21781 atactgctga tggggaaccg gtttactatg gtttttataa aggtcagctt tctatggatg
    21841 ctattgattt tgaaacaatc aaggataagg ctaatgagtt agctactact aatgtgaatt
    21901 ttgctgcgac agctagttca gacactgcaa ctaagggtcg ttatggtgct attgtctatg
    21961 gttcggatgc tgaaaagttg aagaaattga agggtcaact aaacatggct gctgcagggt
    22021 agaaggaggg cgaaagctct cttttttacc gtttttagaa aggaaagtat atggctaagg
    22081 ttcaatttac aatcaaaaat gacaagggag aagatgtcct aaaaaagagt aaggaaatca
    22141 caactcgtga ttatcgtgac tatttggtca tgaacgattc cttgacaagt gatttatctg
    22201 aggtagagaa gcttgataag cagttgggat ttattgctag tttgtttgat gatgtgacag
    22261 tagaacaatt actagaatac acagattttg caaaagtgat ttctattttt acagacattt
    22321 atgctcatct ggtaggtgat gtagacccaa aggaaaagag ctagcacctg ggcaagctat
    22381 ccaacgtttt tatggcttta tcaagcaagt tacagagggt ccttatggaa tgagtattcg
    22441 cgacgtgatg gatacgagtt gggaggactt gatgggcgtt cttggaagta tgaaaacgag
    22501 tgaacaagaa gaagtggttg acttggcaga ttttttagag agtctctaag tcaggacttt
    22561 acaaataaaa gacttaggaa tataatagag ctatataagg gaggtggaag atatgtctcg
    22621 aaaagaaaag gcgcgtagaa gatattggtt ttggtttacc atctttatcc tttatcttgc
    22681 ttttggcttg tattgtattt gtacaaattt tggagatacc ttgggtatgc ttttattatc
    22741 accctttatc tttgcttcct tgccgctcta tgcttatcta tttcttggtt tgtttatctg
    22801 ggcttttatg agcctggcaa tggatgattt ttataaagaa tgatgaaaag tccgtaaggg
    22861 cttttttctt tgctctgagg agttaggaag gagaacaaaa tggcaagcgg tacgccgtta
    22921 ggagcaatgt atatcgagct agggctggac gtgtcgaatt tcaatcctac tctaactggt
    22981 gcaaaaaacg ctgtaaagta ctttcaaaat actgtccgtt ctttggacag tactttaaaa
    23041 gggaatgaaa aaaatgctag cttacttcaa gcgaaataca agactttggg acaagccatt
    23101 gattcacaac gtaaagtttt ggatgagatg aagaaaagtt ttgacaagct tgaaccgggg
    23161 acagctaagt ttgataaggc tgctgctgat attcagcgcg agaatgccaa gttagcagca
    23221 atggaaggtc agctacgtgg tgttgaaaaa gctttgcaag atgtagggcg tgaaaatagt
    23281 tggtctggta agatggacgc cttgagtgag aaatttgcta aaggtggaaa aaaattgcgg
    23341 gctatgggtg atgcaatgaa gcctgtatct gctgctttat tagctggttt tacactttcg
    23401 actaagaagg cgattgactt tgaaagtcag atgaatacaa ctaaatctct gttagctgat
    23461 actattccaa cagcggatga actgaacagt accacacaaa agctggggga gagttcaaaa
    23521 aattgggcga aacagtatgg gatttctact gcatctatca atgaagggat gcaagaaatt
    23581 atcaagaaag gttttgatgc caatcaaaca atcgaagcca tgcctgctat cctagacgct
    23641 gctaaggcgt ctggtgagga tttcaatacg gttatgaacg ccactacgaa tatcttgcaa
    23701 caatttgggc tatctactca ggatacagag cgagtaacca atagtttaac ctttgtcgcc
    23761 aataaaacgg ctgctggttt cgctgatatg ggggctgcaa tggagtatgt tggtcccgtt
    23821 gctaaaaacg ttggtatgga cttggaagag actgcagcta ctgttggtct tttgtccaat
    23881 aatggtattg ctggtgaaaa ggctggtacg gctcttcgtg gagctttaac aaggctgctg
    23941 aaaccatcta agcaaaatgc aaatgcaatg gcaaaacttg gtttttctgc agaagaattt
    24001 cgtagtggtg cgattaagct acctgatatt ttggatcgta tcaaaaagaa tacggaagga
    24061 atgacagatg ctcagaaatc ggccttgatt gctacagcct ttggtacaga agctcagaca
    24121 gcgatgaata tcttggttga ccaaggtgga gatgctttgc gtaatcttgc taacgaaacc
    24181 aagggggcga caacttatac taaggattta gcaaatgaat tatctaagtc ttctaaaaat
    24241 ggtgtggaac gttttaaatc tagtttagaa gttttacaga ttaacattgg tcagaaatta
    24301 ttacccttac taactcctgt gattgagaag gtgaaccaat ttatcgactg gttaagtaag
    24361 gctcctccag ccgttcagag cttggcggtt ggtattggtg gttttttggc tctaggatat
    24421 cctttattga actttctagg caatggtgcg acggctctta gtattttgat aaaacatgct
    24481 ggtaaattag gaggactttt aaagggcggt atcggtcttg ctaaattgac agagcgtgct
    24541 ggtaaattaa aaggactttt acaaggtggt atcggtcttg ctaaggtagg aagtgaagcc
    24601 actgttttag cggaaggagc tggtgttgct gcatctagtt ctggtctctt gtctggagct
    24661 attggggctt tgacaaatcc gattggtttg ctggtgggtg gtgttgcttt actagctgga
    24721 ggattggtct atctagggca taagaaagac gaggcgcgta ttaaggctga ggaatttggt
    24781 tctcagttga gtgatactgc aaggaaagaa ttaagagact tccaaagtaa ggtagatgaa
    24841 accagcaaag cagttgctaa ctttggtaca catgctggag atgctgagaa agtctctggg
    24901 gcttttaaaa agctttatga tgagattgct gctggtgccg aaaagtctaa ccagagaatg
    24961 caggaactga ctggcaaatg gggtctcagt gaggaagacc tcgcaaaagc tagagagaag
    25021 aatgctcaaa tggtttcaaa tgctgaaagc atgatgaaca aaatcaatga tatttaccaa
    25081 cgtcacaatg gagatgctag taagttttct caagaagaaa aagaaatcat cttgaataat
    25141 caaagagaga tgataaaaac tagaatttca atgatgaact tgtcagcaga gcaacagaag
    25201 gctgcgcttc aggctttgaa tggagaaatt ggtagtttaa acgagacgca attgaaacat
    25261 acacgagatg ttttgaagaa gtctctggac gaagaaaaac aactttatga gacttctaaa
    25321 agtgagctga aagagttgct ggaaggaaag gttattgacc aagaaaccta taacaagaaa
    25381 cttcaaactt tagaaacaaa ccaccagcaa actatggaag ccttagggac taagtattat
    25441 caggttatgc aaaatcttga cgctaaggta aaagctcgga ctgggcaaag ctggaactac
    25501 tgggaagaag cgaagaaggt tctggaagaa tatggtttat cttatgaagt gattggtcaa
    25561 aaagctacag aagcttctca aaaaatggga gaatctaata gtatccttgc caaatacact
    25621 agtgatatga gtaaggaaat aagagaagcg aatgatgctt ggtctttact tgttggtaat
    25681 attaatgaga atggcaactt tgagattaaa tccaatgtaa aagaagtaat tggggaagcg
    25741 actaaatctg ctgagggttg ggagcaattg caatttattg ttaaaaatgc gaatatcaat
    25801 tcaaatgccc gggcaacgat tgctgagact ttggttgagt ctggtaagtg gtcaactatg
    25861 acactggaag aaaaacagtt gattgtccaa aatcaggcag gactgcaagc tatctttgat
    25921 agtgaaaacc atcttagaat ctggaacagt atgcctgcag aagtcaagca actccttttg
    25981 gaaaatacag atgtgatgaa taaggctgag gaagcttcca aagctctcta taactacgat
    26041 gccttgactc ctaaacagaa agagttgctg gctacggatg agaatttcag aaaggctgtt
    26101 gcgcgttcga ctgacacgct aacgacttgg aatgcaacca caccgtttac aaaagatttg
    26161 aaactagatc ctacaaatgt tttgaataac gggcaattgt caatagataa aatcatggct
    26221 tggaatctat ctaatccaga gactaaatca ttaaatgcta ctgacaatac tagtgaagca
    26281 gttgatagtg caaaagccag cgtaaactct ccgaaacaag aaaaaccaat tgatttactt
    26341 gcgaaagatc agactgcagg cgttagaaat gagacgagtg cggctattaa tgcgattaag
    26401 caaaataatc cagtagatat ccttgctaaa aatagcactt ccagtactgt tagcgaggtc
    26461 aaaagtggcg ttaatagtat tcaagacaaa acggttacaa tcagcgctcg agataatgct
    26521 tctggtgtgc ttgcaggcat taggtcttgg attactaggg taactggtga tttctttacg
    26581 aatgtttttg ctagtcggca tgctcatggt accaactacc atccaggtgg tcttgctatg
    26641 gtcaatgacc aacgcaatag tacctacaag gaattagtta ctcttccaga tggtaggagc
    26701 ttcattcctg aggggaggga tgttcttctt cctcttccta aaggggctaa agttttgcga
    26761 gcggataaga ccaaacgctt gatgcgtgag atgggtgttc ctaaatatgt tgcaggagtt
    26821 ggcattccga gtgatgctaa attccttcgt gagatggaag aagcacaagc taaaatagtg
    26881 gttcaagcca aaggagagac aaatagcaaa gacagcgata aagtcgtgtc tgagatagcg
    26941 attctgagag caagtatgga gaaaatcctt actgctatcc ttgaaaagcc gtcagatact
    27001 tacctagacg ctgataaaat ttcaatgagc gtctaccaac gtcaaggtgc aatttatgcc
    27061 agggagggaa tgtaatgttt tatatgatta tcaatgggtt taatacatca actatccctc
    27121 actgcgtggt gacggatttt ggtcaggtgg aggcagctaa acctagggtg gttgaagatg
    27181 ctaacctcta cggagccaac ggaagttatc gggtgctgga tggtggctat gagagttatg
    27241 aacggacttt ttcgttctat attcctaagt tactggatgt ttctactatc gtggagaaat
    27301 ttcagcctaa ggacaatgtg ctagagttta gctaccagtt ggggtctgta ttttatgcgg
    27361 attttattgg tgcaacctat agccctcatg ggatgcatgc ctggaaacta gaaatcaagt
    27421 tgaacatgca accgttccgt tatcagaaaa atgttgctcc tcttatcttt gccgcaagtg
    27481 gaaatatcaa caatccaggc tctgtctata gcgagcctgt gattgagatt gagggagacg
    27541 gtgatatttc tttgactatc ggaaggacaa ctatgcactt gaccgttaga cgaaaagtga
    27601 ccattgattg taggcataag aaacagaata tctataatgc agatggcgcg gttcaaaata
    27661 ctttacgtaa acgtggaggc ttctttgagt tggcagttgg taataacagt ctggtcttta
    27721 ctggttcggt tcgcaaggtc acggttcggc cgaattggag gtatatctta tgatttatct
    27781 tactgaaggc aatacacctt taaatgaggc ctacaatgat gaaattgtcc atttggggaa
    27841 caatacctat caactgacct ttcgttttcc tacatcggat accaagtggg aattactgaa
    27901 agaggaaact tttctgactg cagatgacct gcatggtgag caggattttt atatttttga
    27961 ggttgaaaag caacaaggat atattcaagt ctatgccaat caggttatca gtttgttaaa
    28021 taattacatc gttagctcta tcgaggttga ccgtgtcagt gggacaaggg tgttgagtgc
    28081 ttttgctggt agtattacca gagccaatcc tttttctttc ttctctgata ttgatgatag
    28141 gcatacgctc aatatcaagg ataagaatgc catggaggtc ttggccaaag gcaagcattc
    28201 tatccttggt cagtggggcg gagatatggt gcgaaacggc tacaatttac gcttgttgaa
    28261 gaatggcggt tctgaaaatg aatcgctttt tatgtacaag aaaaacttgt ccagctacca
    28321 gcataagacc tcaacgaagt ctttaaaaac tcggataacc tttaaaacga ctgttaaggg
    28381 cgagggagaa aatgcggttg accatgatta tatggtggtg attgacagcc ccttacttgg
    28441 gaactacagc caaatctacg aagatgtggt ggaagtcaat gaccaagacg tgacagatga
    28501 ggctagcttg attgaatacg gtaagcagta ttttcggaca agtatgtgcg acatgctaga
    28561 agataacctt gaaatctcgg ttgtcggtca gagtgatgtt gcggtgcaga tgtttgatgt
    28621 ggtcagtttc taccatgagt ggtacggtct tgatgttcgt aagaaaatca ccaaatatac
    28681 ctattcgcca atggcaaaac tcctaaaatc aataggtttt ggaaccttcc agtccagtct
    28741 tgcaaatgcg atcggtggga ttgtaaatga tgccgttttg aatgaaagcc gaaatctgca
    28801 tcagattttt gaagaacgtt tgaaaaagga aatcgccaac gctgaccgtg cctttgacgc
    28861 tgaatttttc aagcgtgaga aaaccatcac ggatgccatc gaacttgcca aggccaaagc
    28921 ggaagaagtc aagcaagaac tgtctgacac tatcaatcag cgctttaata gctttgacaa
    28981 cgggccattg aaagaagcta agcgcaaggc tgaggaagct ttgcgaaatg ctggcgcaag
    29041 tagttctctt gctcaggaat ccaagcggat tgggctggat tctgttgcta gacttgaagc
    29101 gtttaagtcg cagactacga gcgcacaaac ggctctgtca ggtgacttgg acgctctgaa
    29161 acggactatc gtgaatgata ttcgaccgaa gcaagcacag gttgaagctg agattgccaa
    29221 gcaagttgaa gcacttgttc agaccaaaaa agaactggct ggcgcaagta ccctgcttgc
    29281 acaggaagct aagcggattg agctggattc tgttgctaga cttgaagcgt ttaagtcgca
    29341 gactacgagc gcacaaacgg ctctgtcggg tgacttggac gctctgaaac ggactatcgc
    29401 gaatgatatt cgaccgaagc aagcacaggc tgaaactgag attgccaagc aagttgaagc
    29461 acttagccgg actaaaaatg aactggctgg cgtgaagtca gcgcaagcga cgtatgagga
    29521 gacgacgact cgtagactgt cagaactgac caacttggcc aatggtaaag ccagcaagtc
    29581 agaactcacg cagacagctg aggagctagc tagtcggatt gcgagtgtac aggcatccgg
    29641 tcgaaatcta ttcttgaact cactattcaa gcaggatatt ccaaaaacag gaatttggac
    29701 aacgagtaca tatacggcta ctatcgatag cgaaagtaag tatcttggac acaaggctct
    29761 taaaattata ggtttgaatc catctggccg tgatggaggt aatcccaagg ttacttatcc
    29821 agctctgggt caattcggga aagtaattcc cggaagtacg actaatcaag atgtaaccat
    29881 tagtttttat gctaaggcaa ataaaaatgg aataatgctg agatctcgat tagggaatat
    29941 cggatataaa actggaaatg tgacattgtc gacagaaatt aagcgatatg ttgtccatat
    30001 tccaaaaggt tggacaaacg aatccaagca gaccacaaat gaatggttgt tcaatttcaa
    30061 ccaggaagga accgtttgga tttggatgcc gaagttcgaa ataagcgatg tagatacttc
    30121 ttattcagaa gctcctgaag atgtcgaaag ccaaatctca acagtcgaat cgacctttaa
    30181 acagcgtgct gattcactcg acgctggtgt gaatcgtctg actgaaggcc ttagaaccaa
    30241 agtggatatc agcgcactca acgtgactgc tgagaatatc cggcaatctg tgaagagtct
    30301 tgagacagac acgcagaaca agctaaatca gaagttgagt caggctgaat ttgaggtgcg
    30361 agccggctct atccgtcagg aaatcctgaa cgcaaccaag gataaagcca gcaagtcaga
    30421 actcacgcaa acagctgagg aactggctag taagatagcg agtgtgcagg tcgggggtag
    30481 gaattatatc cggggtacaa agcgcatgat gctagccaga ggattgtggg catcaggtac
    30541 ttttagaccg tcaggcgctg ggacggcaaa gacgattgat gtatcagaca gtccagcaac
    30601 tggctttgat aaagcgatac gattgacctc aagcaatgct agagaccaaa taggtattgc
    30661 tcaagatgga tttcatatct cgcaaggcac atacacgatg tcttgttggg tcaaaggcag
    30721 aagaggtcaa aaagtcaagc tacaaactta ttggcaagtc aatgataatt cgggtatttc
    30781 gcccatcttt acattaaagg atgaaaattg gacaaagcta tcgtttacta gcgctagaaa
    30841 tagggctgga gtcgcatcaa ttggctatgt gtatctcgta aatgctgaag tcggagaata
    30901 tttagatgtt cttgcgcccc agctggaaga cggaagtttg gcaacaagct caaaagaagc
    30961 tcctgaagat atagaaggtc agatttcaac agtagaatcg acctttaaac aacgagccaa
    31021 ctcgctcgac gctggtgtga gaagcttgac tgaagggctt agaaccaaag tggatatcag
    31081 ctcactcaat gtgactgctg aaaatatccg gcaatctgtg aagaggcttg agacagacac
    31141 gcagaacaag ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ctggctctat
    31201 ccgtcaggaa atcctgaatg caaccaagga taaagccagc aagtcagaac tcacgcagac
    31261 agctgaggag ctagctagtc ggattgcgag tgtgcaggca tccggtcgaa atctattctt
    31321 gaactcacta ttcaagcagg atatttcaaa aacaggaatt tggacaacga gtacatatac
    31381 ggctactatc gatagcgaaa gtaagtatct tggacacaag gctcttaaaa ttataggtct
    31441 gaatccatct ggccgtgatg gagggaatcc caaggttact tatccagctc tgggtcaatt
    31501 cgggaaagta attcccggaa gtacgactaa tcaagatgta accattagtt tttatgctaa
    31561 ggcaaataaa aatggaataa tgctaagatc tcgattaggg aatatcggat ataaaactgg
    31621 aaatgtgaca ttgtcgacag aaattaaacg atatgttgtc catattccaa aaggttggac
    31681 aaacgaatcc aagcagacca caaatgaatg gttgttcaat ttcaaccagg aaggaaccgt
    31741 ttggatttgg atgccgaagt ttgaaataag cgatgtagat acttcttatt cagaagctcc
    31801 tgaagatata gaaggtcaga tttcaacagt agaatcgacc ttcaaacaac gagccaactc
    31861 gctcgacgct ggtgtaagaa gcttgactga agggcttaga accaaagtgg atatcagcgc
    31921 actcaacgtg actgctgaaa atatccggca atctgtgaag agtcttgaga cagacacgca
    31981 gaacaagcta aatcagaagt tgagtcaggc tgaatttgag gtgcgagccg gctctatccg
    32041 tcaggaaatc ctgaacgcaa ccaaggataa agccagcaag tcagaactta cgcagacagc
    32101 tgaggagctc tctagtaaga tagcgagtgt gcaggtcgga ggaatcaacc tcttgcgtaa
    32161 tactgcgagt ttgttgattg gcgatcgttc aaaaggatgt tggatgagtg caagcggagg
    32221 aaatgggcga gcgattagcg tagaagtttt ggatcctccc aaaaaaatga taaaaaacat
    32281 gattcgtgtt attgaaaata cgaatggtgg aaataaagat ttaactcaac ttgttagatt
    32341 gcgaattggt gaaaagtaca cgatttcttg ttatgcaagg attgctagcg atagcccaaa
    32401 tgcaaacgtg aacttgctat ttcgttcgtg ggcaaataat accgatttaa atcgcaaatt
    32461 tcagaaatcc atctctcata aaaattggca aaaatattca tttacattta ctgctgatgc
    32521 aattgaaaat tcaattcaat ttggacaatc tggcgcagga attatcgaaa tatgcgctcc
    32581 gaaaatcgaa tcaggaacgt tagcgactga ttacagcgaa gctcctgaag atatagaagg
    32641 tcagatttca acagttgaat cgacctttaa acaacgagcc aactcgctcg aagctggtgt
    32701 gaatcgtctg actgaaggcc ttagaactaa agccgatatc agctcactca atgtgactgc
    32761 tgagaatatt aggcagtcgg tgaagagtct tgagacagac acgcagaaca aactaaatca
    32821 gaagttgagt caggctgaat ttgaggtgcg agctggctct atccgtcagg aaatcctgaa
    32881 cgcaaccaag gataaagcag ataagacttt agttgtatct gaagctggga aattgcgtga
    32941 agaattttca aaaatgaagg tgggaggacg gaatctatgg ataaaatcca agacggttgg
    33001 agctgtaatt gaaaaattac ctgaaaacca cgtcacaggt caaaaagaat gctataggct
    33061 agagaacaac tctactttaa cgttcaacct tgaaccagat ttcagctcaa ggttgtatca
    33121 aaaagttact tttagcgctt gggtcaagta cgaaaatgta gtccaaggtc gaaatttttg
    33181 gaatgtattt aattgcttca aacattatct ttttagaaaa aatagtgaga ccggagtaca
    33241 gagtggtcca gattatgcta cgcttggtat gtataaaggt tcggcagatt ggaaatatat
    33301 tacattcact tatgactact ctgaaaaaac aaattttgat caattgaaga catcattgcg
    33361 attcaatctt gaaggtgcta caagcggtac agcttgggta acaggaatca aggttgaaat
    33421 cggtagtgtg gcgacggact ggagtcctgc gcctgaggac gctgatggtc tcatcactga
    33481 agctaaggct acctttgagc ggacagctca gggcttgcga accgatttat cagctattca
    33541 ggaatatgta aataaagacg gtcagcgaca ggaagcccta cagcgctata ctcgtgagga
    33601 gagcacgaga caagcgacag cagtccgtga gctggtcaat cgtgatttcg ttggtaaggc
    33661 tacttatcaa gaagatgtta agggtatcaa tcaaaggatt gaagctgtta aaactagtgc
    33721 gaataaagac atcgctagtc aaatcgctag ctatcgtcaa tctgtagatg gtaagttcac
    33781 ggatatttca agtcagataa ctacttataa gcaagatgtg ggcggtcaaa tcagtggcct
    33841 ttcaaataga cttacaagca gtgagcaagg aaccactact cagatttcaa atctttcaaa
    33901 tcggataaac agtaataaac aaggcacaga taatcagatt tcaaatttaa agactcaggt
    33961 cgctacaaac aaggataatg ctgaacgaca aatgggtaga atatctgatc aggtttctgc
    34021 aaacaaagcg aatgctgata gtcaatttgc gaatgtgacc aatcaactag tgcgaaaagt
    34081 agagactact gacttccagc gtgttaagga aaccagtaaa ctttacgagc ggattttggg
    34141 caatactgaa aatggaattg cggataaggt tgctcgcatg gctctgacca atcaactgtt
    34201 tcaggttgag gtggctaaaa atgccagcaa tggtcaaaat ttgttgaaag gcacaaaaga
    34261 cttctcaggg ggttggaaaa acaagggtgc aaattggaaa aagcacgctg aaaaatacaa
    34321 aggtgttgat gtcctattta aaaataattc gtggaatggc gttggacaag agattgatgc
    34381 aaaaattggt gaagtctata cattcagctt atggatgaag agcgactgga agaatgatac
    34441 agtaaatttc tatgtaaata gaaatggatc tgttgagaaa ggttggggtg ttccatctga
    34501 aacatcggtc gctataacaa gtgaatggaa acgttactca tttaccttca aaattactgt
    34561 ggacggcttc atctttcctc gtgtagaacg acttaatcaa aatacaaatc tatatattgc
    34621 aggtcttaaa cttgaaaaag gatcgtatgc aacaccgtac accgaagctc ctgaagacac
    34681 ggacgaagcg attcgctctg ttcaaagcca actaactggc tcatgggcag ttcaaaacat
    34741 caactcggct ggagatatca tctctggaat caatcttggc gccaatggac ataaccgctt
    34801 tgttgggaaa ttgacccaca tcactggaga gaccctgatt gacagagcag tcatcaagtc
    34861 tgccatggtt gataagctca aaacggccaa ttttgaagct ggttcggtca cgactacgat
    34921 attagacgct gaagcggtaa ctgctgagaa gttgaaagtt gacgatgcgc ttattagaaa
    34981 attaactgca aaagatgctt ttattgacca actgatatct aaacgtatct tctctattaa
    35041 ggttgagtcc gtcatttcta gctcaacctt cctagaagcc tatcaaggcc gaattggtgg
    35101 attcacactt ggtcaatttg accagggtgg cggtcgctgg atttcaggtg tcaatcagtt
    35161 ctctgttggt atggggaatg gtgccgggca tggagtccgg acagccttct gggcgaactg
    35221 gggaaataat tggaactatg ccggacctaa agcatggaac gtcaatactg atgggaaaat
    35281 gtactgtagg aatgaagtcg gtttttatga tcaagtggat ttttcgaatt catcgagagc
    35341 aaacttctat gggaatacta ctttttctcg ttctcctgtg ttttcaaatg gtatcgaact
    35401 tggaagtaaa gatgtgcttg gtgatggttg gaatcccaaa ggcggaagga atgcggttgt
    35461 ttggtggaat caggtcggta gcggtagcgt gaagtattgg atggaacaaa aatcagacag
    35521 acgcttaaaa gagaacatca cagatacagc tgtgaaagcc ttggacaaaa tcaacagatt
    35581 aagaatggtt gcatttgatt tcatcgaaaa taaaaaacat gaggagattg gtctaatagc
    35641 tcaagaggct gaaaccatcg ttccaaaaat tgtctcacga gatcctgaga atccagatgg
    35701 ctatctgcat atcgactata ccgctttagt tccttactta atcaaggcta ttcaagaatt
    35761 aaatcaaaaa atagaaaaaa tggagaaaac aatagcatga ataacaacat gttgaccaat
    35821 atcgcactta aagcaattta ggagcttgct cttgaaaata gaaaacgaac acacagattg
    35881 gagaacttag aaaatgaaca cagaacagct taaccaagcc ttacaaatga caattagtga
    35941 aatgtcaaca acttcaacaa attcgatgat tacaagtaat atcttgagta ttcagttgaa
    36001 tgagcaaagg gaagagaatc aaagacttca agcacgagtg gatgagctgg aagctctgct
    36061 tgatgaacaa actaaaccag cagacaaagg agaatagaca tggcagaaac aattcaaaac
    36121 acagataact tactagacct tacaaaaatc acagaaccat ttgatcttgc gagtgctttg
    36181 cgctacatga aagaaaatgg agagttcatt cgttgcaaga atgtaagcga tgacttctat
    36241 atgtatcgtg acgttcaaaa acgtcctgtg atcgtaaatg gccgtcgcca attcaaggat
    36301 gttgaaaccg tttgggcatt caatcagtgg ggtggtacaa tcgcaacaat caacgtagcc
    36361 gttctgttga atcatgaatt ctatatcatg aaatttgatg cagagggcaa tcctgactgg
    36421 acggttccaa cggtagaacc taaagaatag gaggttgtat gccaattgaa gaagctgaaa
    36481 aaatcgctca aagtcaggta gcttgggcga ttttgtttat cttgcttttc tttattatca
    36541 ttcgatatct tatcaagact tcggacaagc gagagaagaa gattatggat ttgcacgagc
    36601 aatcaaaggc cgactctaat agacgagaag agcgtttgat gactcaccta gaaaagacca
    36661 ctacagaatt aaccacaatc actcacacgg tcggagacat tcaaaaagaa atggttcgca
    36721 tgaacgaccg catggaagaa atcgaaaaag gagaataaca aatgcaacaa attactgaaa
    36781 tcatcattgc ttttgcgaca agctttttaa cagtagcagt aggcggtatt gtaaaagcag
    36841 taaaagatta tcttttgcgt aaaggcggag agaaagcggt gatcatcgct gaaattctag
    36901 ctaaaaatgc agttcatgcc gttgagcaag tagcttcaga gactggctat aagggcgaag
    36961 aaaagctgga gcaggctcgt gctaaagtcc gtgctgagct tacaaaatac aatattagta
    37021 tgactgacaa agacttagac accttcgtag agtcagcagt gaagcagatg aatgacgcat
    37081 ggaaaggacg ataggatagg gaatggatat cgatagaaac agactacgaa caggcttgcc
    37141 ccaggttggg gtgcagcctt atcgacaagt acacgcccac tcaacaggca accgtaactc
    37201 aacagctcaa aatgaggctg attaccactg gcggaaagac ccagaattag gttttttctc
    37261 gcacgttgtc ggaaatggcc gtgtcatgca ggtaggacct gtgaacaacg gaagttggga
    37321 tgttgggggc ggttggaatg ctgagaccta tgcagcggtt gaactgattg aaagccattc
    37381 aactaaggaa gagtttatgg ctgactatcg cctctatatc gaattgctac gcaatctagc
    37441 agatgaagca ggcttgccga aaacgcttga tacagggagt ttagctggaa ttaaaacgca
    37501 cgagtattgc acgaataacc aaccaaacaa ccactcagac cacgttgacc cttatcctta
    37561 tcttgcaagt tggggcatta gccgtgaaca gtttaagcaa gacatcgaaa acggcttgag
    37621 cgctgcaaca ggctggcaga aaaatggcac tggctactgg tacgtacact cagacggctc
    37681 ttatccaaaa gataagtttg agaaaatcaa cggtacctgg tattatttcg atggctcagg
    37741 ctatatgctt tcagaccgct ggaagaagca cacagacggc aactggtact ggttcgacaa
    37801 ctcaggcgaa atggctacag gctggaagaa aatcgctgac aagtggtact attttgatgt
    37861 agaaggtgcc atgaagacag gctgggtcaa gtacaaggac acttggtact acttagacgc
    37921 taaagaaggt gccatggtat caaatgcctt tatccagtca gcggacggaa caggctggta
    37981 ctacctcaaa ccagacggaa cgatggcaga taaaccagag ttcacagtag agccagatgg
    38041 cttgattaca gtaaaataaa tagaaaggaa actttctaaa ttgttctttc accgcaggct
    38101 caggcttgcg gttttttatt tgctctgatt tttcaaaaaa gtgcttactt gaagaatagg
    38161 gaggtgtttt gtcaaaaata aaaacagtga ccgaaatcac tgcttatcag ttgtagcaaa
    38221 ttcataaagt ttttctgctg tgagaagtgc cattttgtcc atgcttgttt ttccttttct
    38281 gaggtcagaa acagtagtcc atggaactcc agcaccttgg gaaatagcag atgtagacat
    38341 cgaactgtct aacaattctt gaataacttc tctcatctta tttgtccttt ttatttttta
    38401 gataaatata tacattgatt gcaattataa aaatagctat tgcactaacc attgcatttc
    38461 ctctttccat ttgataaaat agaggtgtaa ggggctttcg ccccaacctc ttagcgttta
    38521 cctttttctt tgacgggctt tcggtttacg ctttttgttt tgccttgcga ccgttattgc
    38581 ggtcactaga cttgcgatag cagttaccgt ttcaggaata ttgtctattg ccttttcaag
    38641 taacctgagc caatcttctt tgttcaactt catcaccccc tttccttatc ttgattatat
    38701 tatatcacgg tacaccgaga aagtcaagcg ttttgatgaa gtttttttaa ttttttc
//
LOCUS       DAVLWA010000011        36106 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLWA010000011 DAVLWA010000000
VERSION     DAVLWA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.011"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            148..282
                     /gene="rpmH"
                     /locus_tag="VYN80_000537"
     CDS             148..282
                     /gene="rpmH"
                     /locus_tag="VYN80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1540491.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            566..1459
                     /locus_tag="VYN80_000538"
     CDS             566..1459
                     /locus_tag="VYN80_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1540492.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVGGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATLEY
                     KLETIPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            1764..2537
                     /locus_tag="VYN80_000539"
     CDS             1764..2537
                     /locus_tag="VYN80_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1540493.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            2537..3097
                     /gene="rnmV"
                     /locus_tag="VYN80_000540"
     CDS             2537..3097
                     /gene="rnmV"
                     /locus_tag="VYN80_000540"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1540494.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            3169..3990
                     /locus_tag="VYN80_000541"
     CDS             3169..3990
                     /locus_tag="VYN80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540495.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLDTS
                     RTRRPEYAELILEEHMSQIIEKEVYSTNDMLLIRLFFYQMLIRKDLAKFINQIEKLML
                     FLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDCFNDYLSCLQEIMDKTQDYQKKP
                     LVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGDGFLVKKLTEEWQEDVKKYL"
     gene            4310..4606
                     /locus_tag="VYN80_000542"
     CDS             4310..4606
                     /locus_tag="VYN80_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1540496.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            4683..6713
                     /locus_tag="VYN80_000543"
     CDS             4683..6713
                     /locus_tag="VYN80_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1540497.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     LGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            6715..7356
                     /locus_tag="VYN80_000544"
     CDS             6715..7356
                     /locus_tag="VYN80_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540498.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            7349..7642
                     /locus_tag="VYN80_000545"
     CDS             7349..7642
                     /locus_tag="VYN80_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540499.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            7893..8765
                     /gene="rsmA"
                     /locus_tag="VYN80_000546"
     CDS             7893..8765
                     /gene="rsmA"
                     /locus_tag="VYN80_000546"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1540500.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            8767..9645
                     /gene="rsgA"
                     /locus_tag="VYN80_000547"
     CDS             8767..9645
                     /gene="rsgA"
                     /locus_tag="VYN80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1540501.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            9656..10312
                     /locus_tag="VYN80_000548"
     CDS             9656..10312
                     /locus_tag="VYN80_000548"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1540502.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            10275..10937
                     /locus_tag="VYN80_000549"
     CDS             10275..10937
                     /locus_tag="VYN80_000549"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1540503.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            10939..12195
                     /locus_tag="VYN80_000550"
     CDS             10939..12195
                     /locus_tag="VYN80_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1540504.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            12185..13126
                     /locus_tag="VYN80_000551"
     CDS             12185..13126
                     /locus_tag="VYN80_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1540505.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            13393..14220
                     /gene="purR"
                     /locus_tag="VYN80_000552"
     CDS             13393..14220
                     /gene="purR"
                     /locus_tag="VYN80_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1540506.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            14287..15537
                     /locus_tag="VYN80_000553"
     CDS             14287..15537
                     /locus_tag="VYN80_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1540507.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            15663..16457
                     /gene="pflA"
                     /locus_tag="VYN80_000554"
     CDS             15663..16457
                     /gene="pflA"
                     /locus_tag="VYN80_000554"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1540508.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VYN80_000555"
     CDS             complement(16532..17458)
                     /gene="yidC"
                     /locus_tag="VYN80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1540509.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(17536..17814)
                     /locus_tag="VYN80_000556"
     CDS             complement(17536..17814)
                     /locus_tag="VYN80_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1540510.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17848..18588
                     /locus_tag="VYN80_000557"
     CDS             17848..18588
                     /locus_tag="VYN80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1540511.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            18644..19327
                     /locus_tag="VYN80_000558"
     CDS             18644..19327
                     /locus_tag="VYN80_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1540512.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            19439..20431
                     /locus_tag="VYN80_000559"
     CDS             19439..20431
                     /locus_tag="VYN80_000559"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1540513.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            20496..21035
                     /gene="rsmD"
                     /locus_tag="VYN80_000560"
     CDS             20496..21035
                     /gene="rsmD"
                     /locus_tag="VYN80_000560"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1540514.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            21025..21513
                     /gene="coaD"
                     /locus_tag="VYN80_000561"
     CDS             21025..21513
                     /gene="coaD"
                     /locus_tag="VYN80_000561"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1540515.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            21497..22534
                     /locus_tag="VYN80_000562"
     CDS             21497..22534
                     /locus_tag="VYN80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1540516.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            22592..22822
                     /locus_tag="VYN80_000563"
     CDS             22592..22822
                     /locus_tag="VYN80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1540517.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            22846..24129
                     /gene="murA"
                     /locus_tag="VYN80_000564"
     CDS             22846..24129
                     /gene="murA"
                     /locus_tag="VYN80_000564"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1540518.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            24122..24310
                     /locus_tag="VYN80_000565"
     CDS             24122..24310
                     /locus_tag="VYN80_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1540519.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            24349..25173
                     /gene="endA"
                     /locus_tag="VYN80_000566"
     CDS             24349..25173
                     /gene="endA"
                     /locus_tag="VYN80_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1540520.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            25464..26795
                     /locus_tag="VYN80_000567"
     CDS             25464..26795
                     /locus_tag="VYN80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1540521.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(26993..27063)
                     /locus_tag="VYN80_000568"
     tRNA            complement(26993..27063)
                     /locus_tag="VYN80_000568"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27029..27031),aa:Cys,seq:gca)
     gene            27479..31090
                     /gene="rpoB"
                     /locus_tag="VYN80_000569"
     CDS             27479..31090
                     /gene="rpoB"
                     /locus_tag="VYN80_000569"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1540522.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            31123..34800
                     /gene="rpoC"
                     /locus_tag="VYN80_000570"
     CDS             31123..34800
                     /gene="rpoC"
                     /locus_tag="VYN80_000570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1540523.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            34911..35324
                     /gene="ndk"
                     /locus_tag="VYN80_000571"
     CDS             34911..35324
                     /gene="ndk"
                     /locus_tag="VYN80_000571"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1540524.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
BASE COUNT        10606 a         6921 c         8419 g        10160 t
ORIGIN      
        1 tagggcttgc attcgaaaaa gctggaaaca tttgcctaga gctattgaca aaagtgaaag
       61 aatcagatag aatagtaaag tatgtttagt aactgatcac tttatagccg gctaaacgaa
      121 tacaaaatct atgaggaggt attcatcgtg aaacgtactt atcaaccaag taaacttcgt
      181 cgtgcgcgta aacacggatt ccgtaaccgt atgtcaacta aaaatggtcg tcgcgtattg
      241 gcagctcgtc gtcgtaaagg acgcaaagtt ttggctgcat aatccaaacg aattctatca
      301 aaaatcagta ggaactcgag tctactgatt tttatttttg taaaaaagtt cagtagatgc
      361 aaatggattc ggaagcgatg ttatagtaga ttgaaactag aatagtacac ctctgtttct
      421 aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta ttattttatt
      481 ttactataaa gttgaagtag gtggagatgg tacagcaaca atcgtcttta aagatggttc
      541 agctattaca attccaggaa atcaattggt agcacaagat ccaaaagcac aagatagcac
      601 taaaccgact gctgaaaaat caactgttaa agcacctgct caaagagtag atgtaaaaga
      661 tataactcat ttaacagatg aagaaaaagt taaggttgct attttacaag caaatggttc
      721 agcattagac ggagcgacaa tcaatgtagg tggagatggt acagcaacaa tcacattccc
      781 agatggttca gtagtgacga ttctaggaaa agatacagtt caacaatctg cgaaaggtga
      841 atctgtaact caagaagcta cactagagta taagctagaa actataccag gtggagataa
      901 gggaggcaat actggaagct cagatgctaa tgcgaatgaa ggcggtggta gccaggcggg
      961 tggatcagct cacacaggtt cacaaaactc agctcaatca caagcttcta agcaattagc
     1021 tactgaaaaa gaatcagcta aaaatgccat tgaaaaagca gccaaggata agcaggatga
     1081 aatcaaaggc gcaccgcttt ctgataaaga aaaagcagaa cttttagcaa gagtggaagc
     1141 agaaaaacaa gcagctctca aagagattga aaatgcgaaa actatggaag atgtgaagga
     1201 agcagaaacg attggagtgc aagccattgc catggttaca gttcctaaga gaccagtggc
     1261 tcctaatgct gctcctaaga caacaagtgc accgcaagca actgcaggaa caatgcaaga
     1321 tgttacctac cagtcacctg ctggcaaaca attacctaac acaggttcag catcaagtgc
     1381 agcacttgct agtcttggtc tagtggtggc aacaagtggt tttgctttgc taggaagaaa
     1441 gactagacgt agaaaataga acagctagaa aattctattc tctacttaaa gttagattat
     1501 aagggggatt ttgagaagtc atcaatccta gtgatgggtg agaaaagtga gaacccaaga
     1561 taatcacata ctttagctga ataggaatat tctatcaatg tagccaatct cttctgtctc
     1621 taactgtgga ataggagatg ggcaatatcg gatagaaaag atagcagaat agctctctat
     1681 tgaagagagg aggggaaacc gaaaaattag gtgcccctcc tcttttttgg tataatagaa
     1741 gatagaaaac gaggttagaa gagatgattt ttgatacaca tacacacttg aatgtagaag
     1801 aatttgcagg tcgtgaggca gaagaaattg ccttggctgc tgagatgggt gtgatacaga
     1861 tgaatattgt tggttttgat aaaccgacga ttgagcgtgc cttggagttg gtagatgagt
     1921 atgagcagct ctatgcgact attggttggc atcctacaga agctggtact tatacagagg
     1981 aagttgaggc ttacttgttg gataagttaa aacattccaa ggttgtggct ttaggtgaaa
     2041 ttggcttaga ctaccattgg atgacagcgc ccaaagaggt gcaggagcag gtttttcgcc
     2101 gtcagattca gctatctaag gacttggatt tgccttttgt tgtccatacc cgtgatgcgc
     2161 tggaagatac ctatgagatt atcaagagtg agggcgttgg tcctcgtggt ggtatcatgc
     2221 attcattttc agggacgctt gagtgggcag agaagtttgt ggatcttggt atgaccattt
     2281 ccttctcagg agtggtgact ttcaagaagg caactgacct ccaagaagca gctaaagagt
     2341 tacctttgga caagatgttg gtggaaacag atgcgcctta cttagcacct gtacccaagc
     2401 gtggtcgtga aaataaaaca gcctatactc gctatgtggt cgactttatc gctgacttgc
     2461 gtggtatgac gacagaagag ctggcggtag caacgactgc aaatgcagaa cgaatttttg
     2521 gattggacag caagtaatga aagagaaaat ttctcaagtt atcgtggttg aaggtcgcta
     2581 tgatacggtc aatctcaagc gttatttcga tgtggagacc tatgagacac gaggttctgc
     2641 catcaatgat caggatatag agcgaattca gcgtctacac caacgtcatg gagtcattgt
     2701 ctttacagac ccagatttta atggggagcg gattcggcgc atgattatga cggccattcc
     2761 aacagttcag catgcctttc tcaagcgaga tgaagctgtt cccaagtcca agaccaaggg
     2821 gcgttctctg ggaattgagc atgccagcta tgaagacctg aaaacggctc tagctcaggt
     2881 gacagaacaa tttgaacatg agagtcagtt tgacattagt cgtagcgact tgattcgcct
     2941 tggttttcta gcaggggcag acagccgtaa gcgtagagaa tatctcggag agactctccg
     3001 aatcggctat tctaatggca agcaactcct caaacgccta gagttgtttg gggttacttt
     3061 ggcagaagtg gaagaagcta tgaaatctta tgagtaggaa agatgtagcc gttacaattt
     3121 tttaagtttc acagtatttt tcgaagcagg tagaagagga ggcgtctgat gttaattggt
     3181 caaaaaatta aagagattcg gatagaaaaa ggaattagtc gtccagattt ttgtggagat
     3241 gagcaagaac tgacagttcg tcaactgtcg cgaattgaaa gtggagcttc gcaaccgagt
     3301 ttgcccaagt tagcctatat tgctcgccgg ctaggagttc cagtttatag ccttatgccg
     3361 gatttttcag ctcttccttc tgcttattta gaattgaaat accagatttt acgtgaacca
     3421 atctatggta aagaagagga gtatgataag aaagaagcgt gtttggatac ttctagaact
     3481 agaaggcctg aatatgcaga gttaatactt gaggaacata tgtctcagat catagaaaaa
     3541 gaagtttatt caacaaatga tatgttgttg attcgtttgt ttttttatca aatgctcatt
     3601 agaaaagatc ttgccaaatt tataaatcaa atcgaaaagc taatgctctt tcttttggaa
     3661 cagaaaaagg taactaaatt agaaaacttc tttataatta gagatactct tatttcagga
     3721 atgtgttgtc ttgaaaaggt aggagtaact gattgtttta atgattatct atcgtgttta
     3781 caagaaatta tggataaaac tcaagattat caaaagaaac ctcttgtatt tatgtttttg
     3841 tggaagcaag cattaagagt agaaagagat tttagtttag ctgaatcatt ttatcagtct
     3901 tctaaaacat ttgcgaagct aattggagat ggatttctag taaagaaatt gacagaggaa
     3961 tggcaagagg atgtcaaaaa atatttataa acatagtgaa tcagtgacaa aaatgtcctt
     4021 gtcctcgtat caaaacagtt ctaaagttcg tctttagaac tgttttttta gatataagct
     4081 aaaaataata tgaaatagtt agattttaag gacattgatg tccttgatag tgtgataaag
     4141 atggagtata atgactataa aagttcattt cagggtgcta ttttgacaat ttagagaaaa
     4201 acaaataatt ttagaatatc atttggtaat ataatagtat caacgttgat aatggaaaga
     4261 ggagcaagta actcaacaat cttgtaatca aaaatagagg agtatcaata tgaaacaaac
     4321 agttaaaaag ttagctcttg tagcgagcat tgcagcaaca ttaggtggag gtgtatcagt
     4381 tgcctctgca gccgttcaat acccagaagg tggtgtttgg acttatggtt caggtaacgg
     4441 tggtgcttac tcaaactact atcacccttc aaaataccat agttcaacag ttgttagcag
     4501 aaaaactggt tcatctgaca agggatatgc tggtgctgga gggacttctc gtgcatggat
     4561 tcgtacttct tggggagaga aagttgcatt ctactataat gtttagaatg gtatagccct
     4621 attgtttagc tttacttggt caccttctca ctaagtgacc aagtaacttt ttggaggaaa
     4681 tgatgaaacg tttatttatt ttgatttcaa tggtattggt atcgctttat atggtgataa
     4741 cttccgttga ccatcgagaa gagattttat ttggtaacta tccttctgtt gatgtgacag
     4801 gaatgatgat aaatcaacca gtagctagtc gcgaagaggt gacagaggct ttgagtcact
     4861 tggcggtaga gcataatagt ctcatcgccc gtcgaatcgt tgagccaaat gaagctggag
     4921 aaacactctt tacctatgcc acttatggtg agggagagct tccagaaggt ctgaccattt
     4981 cctccaagga gagtgcagaa acgagtgatt tattagggtc ttacttgatt gtgtcaggaa
     5041 gtttggatgg agtgagctta cagaccacct tgaaagagct tggttatcaa ggctttgttt
     5101 cgaatggaga agatccattt tcgatagtct tactattggc ggccacccct atggtgctac
     5161 tgagtttagc tatttttctg ctgaccttta tgagtctgac cctgatttat cggatcaaat
     5221 cccttcgtca ggcagggatt cgcttaatag ctggtgagag cttgtttgga gttgctctca
     5281 gaccagtgtt agaagatgtg agacagctta tctgctcagt gctggtatcc agtcttttgg
     5341 gattggggat tctctggtat caaggtgcct tgtttatggc aacggtacaa ctggtcatca
     5401 ttgctcttct actttatgga ttgaccttgg cagggatttc taccttacta agtgtcgtct
     5461 atctacttgg tttacaggaa aatagtctgg tggatctatt gaaagggaaa ctccctctca
     5521 aacgtatgat gacattgatg atggtggggc aactcttagc tgtattggtg gtcggatcga
     5581 gtgcgacagc tctcctaccc cactaccgtg aaatgcagga aatggagaga gctagcaata
     5641 aatggagcca gtcctcagac cgttaccgtc tatcctttgg ttggtctagt gcatttgccg
     5701 atgaagaagg aacgcgtaag gataatcgtg agtggcagac atttactgaa gaacggttag
     5761 ccaatacaga ctctttttat attatgagca atgttgacaa tttctcagat ggagcagaag
     5821 tggacctaga tggcaatcgt ctcagtgact acacaccgtt agggaatgtt atctatgtct
     5881 cgccgcgcta tctgatagaa gaaaagatta ccgtttcttc agagtttatg gacaagatgc
     5941 aaaacttgtc tgagggagag tttgggctga tcttgcctga gagcttgcga gagcagtctg
     6001 cctactacca aggattgttt acagattacc tgcaaaactt ttcatctgaa agtgtagaag
     6061 tgacgagtca gaaacactac ctcccacagg taaggctagc ttttacagaa acaggacagg
     6121 aacgtttcct ctataatgat gggtacaaga caacacgcca gtacctaaaa gatccgatta
     6181 ttgtagttct aacgccgcaa gcgactggaa caagacctgt tgcagggatg ttgtggggaa
     6241 ctacggctaa tagtgccttg aaactagatc gatatggaga cagcatcaca gctctaaaag
     6301 agcaaggtct gtatcacaag gtttcttact tggtaaaaag ccagctattt tttgccaagg
     6361 tactaaatga caaacgggtg gagttttact ctctccttat tgggacgatt ttgaccctgt
     6421 ctacggctat cttgttattt gattccatga atcttctcta ttttgagcag ttcagacggg
     6481 aacttatgat taaacgtctt gctggtatga caatctatga gcttcatggc aagtatttac
     6541 tggcgcaagg aggagttctc ttgcttggcc tagtcctatc tagtattttg acaagagatg
     6601 gtttgattag cgctctagtt gtagctttgt ttacgcttaa cgccctcttg attttagtaa
     6661 ggcaggataa aaaagaagaa gctggtagca tggcagtatt gaaaggaaaa taagatgatt
     6721 gatattcaag gattggaaaa gaaatttaat gaccgcgcga ttttctctgg tttgaatctc
     6781 aagctggaga agggcaaggt ttatgcctta atcggaaaga gtggaagcgg aaagacgacg
     6841 ctgctgaata tcttgggaaa gctagaaaag atagatggtg gaagggttct ctatcagggg
     6901 aaagatttaa aaaccattcc tactcgtgag tattttcgag accagatggg ctatctcttt
     6961 caaaatttcg gcctcttaga aaaccaatca atcaaagaaa atttggattt gggttttgtt
     7021 ggtcagaaaa tctcaaaagt agaacgtttg gaaaggcaag tgggggcttt agaaaaagtt
     7081 aatctagggt atttggattt agaacaaaaa atttatactt tatctggggg agaggcccaa
     7141 cgagttgccc ttgctaagac tattttgaaa aatccaccct tgattttggc agatgaacca
     7201 acagcagctc ttgatcctga aaattcagag gaggttatga atctcttggt ggatttgaaa
     7261 gatgaaaatc gaattatcat cattgcgacc cataatcccc tagtctggaa taaggctgat
     7321 gaaatcattg atatgaggaa acttgctcat gtgtgaaaaa atccgtattc gcagggtatc
     7381 tgattatcct agtgccagag gtggtttaga agatatcctc atcatggaaa atatgaccaa
     7441 tcatctcctt ttggttcaaa tccgagtgca tggctatttg cttgattttg ctagtattga
     7501 agggcaaagg caaaagcatt atcgtttgaa aaatttacct cagacggttg aactgacagt
     7561 ggatgatgtg gaggaggatg tggatttgac cctacccgaa aatcgaagtt atcaagaagc
     7621 tgattttttt gatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt
     7681 acttgagtac ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtatgaacg
     7741 tatgtttcga gagaagcact aagtccctct ttaaagattt ccaagactat ctttcttcat
     7801 gaggaaagat agttttttgg tatgattttc attcccaaaa tacaagggga atgtgttaca
     7861 atagtagtaa cagataatag aaaagagaat agatgagaat tgcagattat agcgtgacca
     7921 aggcagtgtt ggagcgtcac ggttttacct ttaaaaagtc ctttgggcaa aatttcttga
     7981 cggataccaa tatccttcaa aaaattgtgg atacggctga aattgatgat caggtcaatg
     8041 tcatcgaaat cgggccaggt attggtgcct tgacagaatt tttggctgag cgtgcagccc
     8101 aagtcatggc ttttgagatt gaccaccgtt tgatgccaat tttggcagat actctgcgtg
     8161 attttgataa tgtgaccgta gttaacgaag atattctcaa ggttgatttg gcgcaacata
     8221 tccaaaattt taaaaatcct aacctgccaa tcaaggtagt ggctaatttg ccttactaca
     8281 tcacgacgcc tattctcatg cacttgattg agagtggcat tcctttttgt gagtttgtgg
     8341 tcatgatgca gaaagaagta gcggaccgca tttcagccca gcctaacacc aaggcttacg
     8401 gtagcttgtc tatcgccgtg cagtattaca tgacagccaa ggttgccttt atcgtgcctc
     8461 gtacggtctt tgtgccagcg ccaaatgtgg attcagccat cttgaaaatg gtgcgtcgtc
     8521 cagagccagc cgtagcagta gaagatgaga actttttctt taaggtttcc aaggctagtt
     8581 ttacccatcg ccgcaagacc ttgtggaata acttgacagg ttactttggt aagactgaag
     8641 aggtcaagga caagctgacc aaggctttgg accaggcagg cttgtcacca agtgtgcgtg
     8701 gggaagctct cagcttggca gaatttgccg gtctagcaga cgcacttaaa gggcaaggac
     8761 tctaagatgc agggacaaat cattaaagcc ttggcaggtt tctactatgt ggagagtgat
     8821 ggccaggttt atcaaacacg cgcgcgtggg aatttccgta aaaaaggcca taccccttat
     8881 gttggggact gggtagattt ctctgccgag gaaaattcag aaggctatat cctcaaaatt
     8941 cacgaacgga aaaacagtct ggttcgtccg cctattgtca atatcgatca agctgtagta
     9001 atcatgtccg tcaaggaacc tgattttaac agcaatttgc tggatcgttt cttggttctt
     9061 ttggagcaca agggcatcca tcccattgtc tatatttcca aaatggattt gttggaagat
     9121 aggggagaac tggattttta ccagcagacc tatggtgaca tcggctatga ctttgtgacc
     9181 agtaaagagg aactcctgtc tttgttaaca ggcaaggtta cggtctttat ggggcagaca
     9241 ggtgttggga agtcaactct tctcaataaa atcgcaccag acctcaatct tgaaacggga
     9301 gaaatttcag acagtctagg tcgcggtcgc cataccactc gagctgttag tttttacaat
     9361 ctcaacgggg gtaaaatcgc agatacacca ggcttttcat ctctggacta tgaagtatca
     9421 agggctgaag acctcaatca ggcctttcca gagattgcta ctgttagccg agattgtaag
     9481 ttccgtactt gtacccatac ccatgagccg tcttgtgccg tcaaaccagc tgttgaagag
     9541 ggtgttattg caaccttccg ttttgacaat tacctgcaat tccttagtga aattgaaaat
     9601 cgtagagaaa cctataaaaa agtcagcaaa aaaattccaa aataaggaga aacctatgtc
     9661 tcaatacaag attgctccgt caattctggc agcagattat gccaactttg aacgtgaaat
     9721 caaacgtcta gaagcaactg gggcagaata tgcccatatc gatatcatgg acagtcattt
     9781 tgtaccgcaa atcagttttg gtgcaggtgt ggtcgagagc cttcgtcctc atagtaagat
     9841 ggttttcgat tgccacttga tggtgtcaaa ccctgagcat catctggaag attttgcgcg
     9901 tgcaggtgca gacatcatca gtatccatgt agaagcaacg cctcatattc atggcgccct
     9961 ccaaaaaatt cgttcactcg gagttaagcc ttcagtcgtt atcaatcctg gcacatcagt
    10021 tgaagccatc aagcacgtcc ttcatctagt tgaccaagtt ttagtcatga cggttaatcc
    10081 aggttttggt gggcaagcct ttctgccaga aaccatggat aaggttcgtg agttggttgc
    10141 tcttcgtgag gaaaaaggtt tgaactttga aatcgaagtg gatggtggga ttgatgacca
    10201 aactattgcc caagccaaag aagctggtgc gaccgttttt gtagcaggtt cctatgtctt
    10261 taagggagat gtcaatgagc gagtacaaac tctcagaaaa caactggact agggttgccg
    10321 tttttgcagg cggagaccgc ggtcattatc ggacagattt tgatgctttt gttggggtgg
    10381 atcgaggctc gctctgggtc ttggaagaag acttgcttct tgctctagca gttggggatt
    10441 ttgattctgt gaccgaagaa gagcgacagg tgattcaaaa acgtgcccag cattttgtcc
    10501 aagcccgacc agaaaaagat gataccgatt tggaattggc tctcttaacc atctttgaga
    10561 aaaatcctca ggctcaggtt actattttcg gtgccttggg tggccgtatt gaccatatgt
    10621 tggccaatgt ctttctgcct agcaatccta agttggcacc ctatatgcat caaatagaaa
    10681 ttgaggatgg gcaaaacttg attacttatt gtccagaagg gaccagtcag ctagaacctc
    10741 gttcagacta cgactatcta gcctttatgc cagttcggga tagccagctg actattcttg
    10801 gagccaagta tgagttgaca gaggaaaatt ttttctttaa aaaagtgtac gcttctaacg
    10861 aatatataga tagggaagtg tcggtaactt gcccagatgg ttatgtggtc gtactgcata
    10921 gcaaggacag gaggtaggat ggaaagttta cttattctat tattaattgc caacctagct
    10981 ggtctctttc tgatttggca aaggcaggat aggcaggaga aacacttaag taagagcttg
    11041 gaggatcagg cagatcattt gtcagaccag ttggattacc gctttgacca agccagacaa
    11101 gccagccagt tagaccaaaa agatttggaa gtggttgtca gcgaccgttt gcaagaagtg
    11161 cggattgaat tgcaccaagg tctgacccaa gtccgtcaag aaatgacaga taatctcctc
    11221 caaactagag acaagacaga ccaacgtctc caagccttgc aggaatcaaa tgagcaacgt
    11281 ttggaacaaa tgcgccagac ggtcgaggaa aaactagaaa agaccttgca gacacgctta
    11341 caggcttcct ttgagacagt ttctaaacaa ctggagtctg tcaatcgtgg ccttggagaa
    11401 atgcagacag ttgcccgtga tgtcggagct cttaacaagg ttctctctgg aaccaagacg
    11461 cgagggattc tgggagaatt gcaactgggg caaattattg aagacatcat gacacctgcc
    11521 cagtacgaac gagaatacgc aacggttgaa aactctagtg aacgagtgga gtatgccatc
    11581 aagttacccg gacaaggcga ccaagaatac gtctatctgc caattgactc taagtttcca
    11641 ctggcagatt attaccgctt ggaagaagcc tatgagacag gtgacaagga tgagattgaa
    11701 cgctgtcgta agtcactcct agcaagcgtc aagcgctttg ccaaggatat caagagcaag
    11761 tacatagcac cgcctcggac gaccaatttt ggagttttgt ttgttccgac agaaggtctt
    11821 tattcagaga ttgtgcgcaa tccggtcttc tttgatgatt tgagacggga agaacagatt
    11881 atcgttgcag gaccaagtac cctatcagcc cttcttaact ccctatcagt tggtttcaag
    11941 acccttaata tccaaaagag tgccgaccat atcagcaaga ctcttgccag tgtcaagacc
    12001 gagtttggca agtttggtgg tattctggtc aaggcacaaa aacatctcca acatgcctct
    12061 ggcaatattg atgaattatt aaaccgtcgt accatagcta tcgagcggac gctccgtcac
    12121 attgagttgt cagaaggcga gcctgcgctt gatctactcc attttcaaga aaatgaggaa
    12181 gaatatgaag attagtcaca tgaaaaaaga tgagctattt gaaggctttt acctaatcaa
    12241 atcagctgac ctgaggcaaa ctcgagctgg gaaaaactac ctagccttta ccttccaaga
    12301 tgatagtggc gagattgatg ggaagctctg ggatgcccaa cctcataaca ttgaggcctt
    12361 taccgcaggt aaggttgtcc acatgaaagg acgccgagaa gtttataaca atacccctca
    12421 agtcaatcaa attactctcc gcctgcctca agctggtgaa cccaatgacc cagccgattt
    12481 caaggtcaag tcaccagttg atgtcaagga aattcgtgac tacatgtcgc aaatgatttt
    12541 caaaattgaa aatcctgtct ggcaacggat tgtccgaaat ctctacacca agtatgataa
    12601 ggaattctac tcctatccag ctgccaagac caaccaccat gcctttgaaa cgggcttggc
    12661 ctatcatacg gcgaccatgg tgcgtttggc agacgctatt agcgaagttt atcctcagct
    12721 caataagagc ctgctctatg cggggattat gttgcatgac ttagctaagg tcatcgagtt
    12781 gacggggcca gaccagacag agtacacagt gcgaggtaat cttcttggac atatcgctct
    12841 cattgatagc gaaattacca agacagttat ggaactcggc atcgatgata ccaaggaaga
    12901 agtcgttttg cttcgtcatg tcatcctcag tcaccacggc ttgcttgagt atggaagccc
    12961 agtccgtcca cgcattatgg aagcagagat tatccatatg attgacaatc tggatgcaag
    13021 catgatgatg atgtcaacag ctcttgcttt ggtggataaa ggagagatga ccaataaaat
    13081 cttcgctatg gataatcgtt ccttctataa accagattta gattaataat ttaagaaaaa
    13141 tgagcatttt ttaggataag aatgttcgtt tttttatgtg aatatggtat aataagtaaa
    13201 agacaaaaat gaatactctt cgaaaatctc ttcaaactag ggtagtatcg ccttgtcgta
    13261 tgtatatatg caggtatatt acagggtttg tcagttctat tgacaatctc aaaacagtgt
    13321 tttgaaccac cagcgaccag ctttctagtt tgctttttga ttttttgaat aaaaatggaa
    13381 taggaaatag aaatgaaatt aagaagaagt gatcggatgg ttgtcatttc caactatttg
    13441 attaataatc cttataaact aactagtctc aatacttttg ctgaaaagta tgagtctgct
    13501 aaatcatcca tctcagaaga tatcgtcatt atcaaacgcg cctttgagga aattgaaatc
    13561 ggtcatatcc agacagtgac tggggctggc ggaggtgtca tcttcacacc gtctatttcg
    13621 agtcaggatg ctaaggaaat ggttgaagac ttgcgtacca agttgtcaga aagtgaccgt
    13681 atcttgccag gtggttatat ctatctgtct gatttgctta gcacaccagc catcttgaaa
    13741 aatattggtc gtattattgc caaaagcttt atggaccaaa aaattgacgc ggttatgacc
    13801 gtagcaacta agggtgtgcc acttgcaaat gcagttgcca atgtcctcaa tgtctctttt
    13861 gtcattgtgc gccgtgacct gaaaattacc gaaggttcaa ctgttagcgt caactatgtt
    13921 tcaggttcaa gtggtgaccg tatcgagaaa atgttccttt caaaacgtag tcttaaggca
    13981 ggtagccgtg tcttgattgt ggatgacttc ttgaaaggtg gcggaacggt caacggtatg
    14041 attagtctct tgcgcgagtt cgactcagaa ctggcaggtg tagcggtctt tgcggacaat
    14101 gcccaagaag aacgtgaaaa gcagtttgac tacaagtcac tcttgaaggt aactaatatt
    14161 gatgtcaaga accaagccat cgatgttgag gttggcaata tctttgacga agataaataa
    14221 gagatagaac taaaggttgg aacgattgtc ccagcctttc tttgcaaaca gaatagaagg
    14281 aagcttatga aaacaccatt tatcaataga gaagagttag aagcgattgt tgccgagttc
    14341 ccgactccct ttcacttgta tgatgagaag gggattcgtg agaaggcaag agccgtcaac
    14401 caagcttttt cgtggaacaa gggctttaag gaatattttg cagttaaggc tactccaact
    14461 ccagctattt tgaaaattct ccaagaagaa ggttgtggtg tggactgctc tagttatgta
    14521 gagcttttga tgagccataa actggacttt ctgggttctg agattatgtt ctcttccaac
    14581 aacacgccag acaaggaata cgcctatgca cgtgaattgg gtgcgaccat taacttggat
    14641 gcctttgaag atattgaaca tctggagaga gtagcaggca ttccagaaat catctcttgt
    14701 cgttataatc ctggaggcgt ttttgaactg gggacagaca ttatggacaa tcctggggag
    14761 gctaagtttg gcatgaccaa ggaccagctc tttgaagcct ttgctatctt gaaggaaaaa
    14821 ggagccaaga cttttgggat tcactccttc ctagcgtcca ataccgtgac ccatctctat
    14881 tatccagagt tggctcgtca gctctttgaa ctggctgttg aaatcaagga aaagttgggc
    14941 atttggctag actttatcaa tctttctggc ggtattggtg ttaattatcg tccagaccag
    15001 gagccgaacg atatcgcctt gattggtgag ggagttcgta aggtgtatga agaagttctt
    15061 acgtcagcag gtcttggtca ggtcaagatt ttcaccgaat tgggtcgttt tatgctggca
    15121 cctcacggtg ctctagtcac aagagtgact cataagaagg aaacctaccg tacctatcta
    15181 ggtgtggatg cctcagcagt caacctcatg cgtccagcta tgtacggagc ttaccatcat
    15241 attagcaacg tgacccatcc agatggacca gctgaagcgg tagatgtggt tggttcactc
    15301 tgtgaaaaca atgataaatt tgcagtcaat cgcgaactgc ctcatacaga aatcggtgat
    15361 ttgctggtaa ttcatgatac aggtgcccac ggattttcaa tgggctacca gtataatgcc
    15421 aaattacgtt ctgcggaaat cctctatacc gaagaaggta aagcccgtca aatccgccgt
    15481 gcagagcgcc ctgaggacta ttttgcaacc ttatatggct tcgattttga agaataatct
    15541 gataatagat tgaaaatgaa attgaaaaac agattgcttt ctaaaaaata ggcaaaaatc
    15601 ttgtttttcc ttcaagtcgt gatataataa aactataaaa cgttttcaag gaaggtaacg
    15661 atatgtctga agaaacaatt gattatggac aagtgacagg aatggtgcat tcgacagaaa
    15721 gctttgggtc agtagatggg cctggtattc gctttattgt ctttttgcag ggctgtcaca
    15781 tgcgttgcca gtattgccac aacccagaca cttgggctat ggagtccaat aagtcacgtg
    15841 aacggacggt agatgatgtc ttgacagagg ccttgcgcta ccgtggtttc tggggaaata
    15901 agggtgggat tacagtcagt ggaggagaag ctctcttgca gattgatttc ctgattgctc
    15961 tcttcaccaa ggctaaggaa caaggaatcc actgtacctt ggatacctgt gctcttcctt
    16021 tccgtaataa accacgttac cttgagaagt ttgacaaact catggctgtc actgacttgg
    16081 ttcttttgga tatcaaggaa atcaacgaag aacagcacaa gattgtcact agacaaacca
    16141 ataaaaatat cttggcttgt gcccagtatc tatcagatat tggaaaacct gtctggattc
    16201 gccacgtgct agttccagga ttgacagaca gagatgatga cttgattgaa cttggtaagt
    16261 tcgtcaagac cctcaaaaat gttgataagt ttgaaattct accttatcac accatgggtg
    16321 agttcaagtg gcgtgaactt ggaattccat attccctcga aggagtcaaa ccaccaacag
    16381 cagatcgcgt caagaacgct aaaaaactca tggataccga aagttatcaa gattatatga
    16441 aacgtgtaca tggatagaaa agaagcctga tggaaacatc gggcttttga tttgcaaaaa
    16501 gacttagcaa atcagctaag cctttttctt cttatctcga acgttgtttt ccagcgttgc
    16561 gatttttgtg ttttttcttg cttgtgatag cagttggttg ttcaggggta acgtcttttc
    16621 gtccacttgg tttagagaaa gcacttgctt ttggtgggtt cttggctagt tcttcacgga
    16681 cttttttgcg aagttttgga cgaacgatat agttgacgat aaactgttgg agaatcatca
    16741 tgaaaccacc gacaacccag taaagtgtga cactagctgg tgagaagagg gagaagacga
    16801 cgatcatgag tgggctcatg taaatcattt tcttgatttg ttctctttgc atttcatctt
    16861 ctactccgtg aagtgaaagg agcgattgaa gatagtaaag gacaccagca caggcaacca
    16921 aaatcatact tggagaacct agaggaatgc ctaggtagct tgcttgagca accccttcag
    16981 tatgttgggc agcaaagtag atagcagaga agaaaggcat ttgaaggagg atagggaaac
    17041 atcctacacc gccaaacatg ctgataccgt gctctttttg agcagcaaag agagcttgtt
    17101 gggcttcgag tttttcttct tgagtagtcg cttctttgag acgcgtttgg tgtggctcaa
    17161 ggacgtgctt gagggcgttc atcttttcag agtgaagcgt tgccttccat gattggtaga
    17221 taccaagtgg caagataatc aagcgcacga taatggttac gatgatgata gcgacaccaa
    17281 agcctagacc tttatcagta gcgaagtact tgatagcttc agccataggg gctccgatgg
    17341 tattccaaat aaatcctgtt ggctgacctg tggttttatc gacattgaca caaccagtca
    17401 agacaagcaa catagccgct cccatagctg agagtgcaaa acgtttaata gatttcactg
    17461 tttttattcc ttctttaaaa attatacctt tctattctac tgttttttta caaaatatac
    17521 aatagttctg gagacctaat ttgcgatttt aaagtcagag tagggaggaa agttgcttag
    17581 tttgacatct aagtagctta cttttgaaaa aggtgtcggt ccttttcgga tttcttggat
    17641 aaattttgcc atgatagcag atgagtctgc ttgggctaag atttccactg tgccatcgtc
    17701 gttattccat actcgacctg tgatgccacc aatttcaagt gccaagctgt aaacacccca
    17761 acgaaagccg actccctgta ccctaccttg ggcaatcatt ctaacctttt gcataccaaa
    17821 cccctttgat tgtgttataa tatttctatg actattataa cctcaaaagc caattctgtg
    17881 gtaaaaaatg ccaagaaatt acgccaaaaa aaataccgca agtctgccta cttgattgaa
    17941 ggctggcact tgtttgaaga agctgtccaa gctggagtga cgattgagaa aatctttgcc
    18001 ctagaaaatt atcgagatca gttagctact ttttcgcaaa ctgtctgggt gtcagaggat
    18061 attttgctag atttggcgga ttctcagact ccacagggaa ttgttgccgt ggttcaaaaa
    18121 gaagaagtag gacaagctga tttgagtcag ggcaagttct tgtttttgga agatgtgcaa
    18181 gatcctggta atgtaggaac tatcattcga actgcggatg cagcaggttt tacaggagtg
    18241 attgtttcag ataagtcggc agacatctac agtctcaaaa ccctacgttc catgcaaggt
    18301 agtcatttcc atctgcccat ttaccggatg actagtcaag cgcttcttga cgaaactaaa
    18361 aaggtagcta tcccagtgct agcaacaacc ctatctaaag attctgttga ttacagagaa
    18421 ctgccttcta tagaaaattt tgtactagtt atgggaaatg agggtcaagg aattagtccc
    18481 cttatggctg aaagtgcaga ccagttggtc catattagca tgaaggggca ggccgagagt
    18541 ttgaatgttg cggttgcagc cggtatttta atcttccatt taagctaatt ttaacttttt
    18601 ttgttataat caaggaaaga tgttcacaga aaaggagaaa tggatgaatc acactattat
    18661 acatgaccgt gcaggtctca atcaatttta cgctaaggtt tatgcctttg ttggtctggg
    18721 aatcggacta tctgctttgg tatcaggcct tatgttgacg gtctttcagt ctcagttggt
    18781 ttactttttg atgcaggggc gtctctggtt gaccattgct acttttgcgg agttggcttt
    18841 ggttttcgtt gccagtagca tggcttcaag aaatagtcca gctgctcttc cagtattttt
    18901 actttactcc gttttaaacg gctttaccct tagttttgtg gtggctttct atactccggg
    18961 tacagtttta tctgcctttg tatcgagcgc ccttctcttc tttgtcatgg cggcagttgg
    19021 tatttttacc aagaaagact tgagtgggat tggtcgagca atgatggcag ctctcattgg
    19081 tttactgatt gctatggtag tcaatatttt cttggctagt ggcttctttg attatatgat
    19141 tagcgtagcc atggttttgg ttttttcagg gttgattgct tgggataacc aaaagattcg
    19201 cctcgcctat gaacaatcac aaggtcgtgt agcgacaggc tgggtggtgt caatggctct
    19261 cagcatctat cttgatttta tcaatctttt cctaagtatt ctacgtatct ttggtcgcaa
    19321 tgactaaaag gtctatgaaa tcagtgtttt gagtgacact gatttttttt aaattttctc
    19381 ttttcttttc ttggaaatag gtgtataata cgtttattaa atttttgagg agttgtctat
    19441 gaagaaaagt tttatccatc aacaagaaga aatttccttt gtcaaaaaca cttttaccca
    19501 gtatttgaaa gataagctag aagttgtcga agttcaaggt cctatcttga gtaaggtcgg
    19561 tgacggaatg caggacaacc tgtctggtgt ggaaaatcca gtatcggtca aggttctcca
    19621 aatccctgat gctacttatg aagtggtgca ctcacttgct aaatggaaac gccacacctt
    19681 ggctcgtttt ggctttggtg aaggagaggg tctctttgtc cacatgaaag cccttcgtcc
    19741 agatgaggat tccttggatg caacccactc tgtttatgtt gaccagtggg actgggagaa
    19801 ggttatccca aatggtaagc gtaacatcgt ttatctaaaa gaaacagttg agaagattta
    19861 taaggctatt cgcctgactg agctagctgt tgaagcccgc tatgacatcg agtctatctt
    19921 gccaaaacaa atcaccttta tccatacaga agaattggta gaacgctacc cagacttgac
    19981 acctaaagaa cgtgaaaatg cgatttgtaa agaatttgga gccgtctttt tgattggtat
    20041 cggtggcgag ttgccagatg gtaaaccgca cgatggacgt gcaccagact atgatgactg
    20101 gacaagcgag tctgagaatg gctacaaggg tctaaatggt gatattcttg tctggaatga
    20161 gtctttaggt ggagcctttg agttgtcttc tatgggaatt cgtgtagatg aagaaactct
    20221 tagacgtcag gtggaaatta caggtgatga agaccgcttg gaattggaat ggcacaagtc
    20281 tttgttgaat ggcctattcc cattgacaat cggtggagga attggacaat ctcgtatggc
    20341 catgttccta cttcgcaaga gacacatcgg agaagtgcaa acaagtgttt ggcctcaaga
    20401 agtccgcgat acttacgaaa atattttgta gagaatcgaa ccgcaaggtt cggttttctt
    20461 tctctttttg tctataattt ggtataataa acagtatgaa aatcgtatca ggaatctatg
    20521 ggggacgtcc cctcaagaca ctagaaggca agacaacaag acctacttcg gataaggtta
    20581 ggggagccat ttttaacatg attggtccct actttgaagg gggacgagtc ttggaccttt
    20641 atgcaggtag tggtggttta tctatcgaag cagtatcgcg tggcatgtcc agtgctgttt
    20701 tggtggagcg agaccgtaag gctcagacca tcgtggctga aaatatccag atgaccaagg
    20761 aagttggaaa atttcaactc ctcaagatgg atgcagagag ggcattggaa caggtatctg
    20821 gggaatttga cctcgttttc ttagaccctc cctatgccaa ggaacaaatc gtagcagata
    20881 ttgaaaaaat ggctaagaga gagctttttt ctgaagatgt tatggttgtg tgcgagacgg
    20941 ataaggccgt tgaacttcca gaagagattg cctgtctggg tatctggaag gaaaagattt
    21001 atggaattag taaggtgaca gtctatgtca gataagattg gcttattcac aggctcattt
    21061 gatccgatga caaatgggca tctggatatc attgaacggg cgagcagact ttttgataag
    21121 ctttatgtgg gtattttttt taatccccac aaacaaggat ttctccctct tgaaaatcgt
    21181 aaacgggggc tagaaaaggc tttgggacat ttggaaaatg ttgaagtcgt ggcttctcat
    21241 gatgaattgg tggtcgatgt tgcaaaaaga ctgggggcta ctttcctagt acgggggttg
    21301 aggaatgcgg cggatttgca atatgaagct agttttgatt actacaatca tcagctgtct
    21361 tctgatatag agactattta tttacatagc cggcctgaac atctctatat cagttcatca
    21421 ggtgttcgag agcttttgaa gtttggtcag gatattgcct gccatgttcc cgagagtatt
    21481 ttggaggaaa taagaaatga aaaaaaagat tagatggccc ttatacgtca ttgcggcctt
    21541 gattgtgact ttcttggcat ttgtagtgcc cttgccttat tatatagagg ttccaggtgg
    21601 ttcggaagat attcgccaag tccttaaagt aaatgacaca gaagataagg aagctggtgc
    21661 ctatcaattc gttacggttg gtgttcaaca tgccacttta gctcatatga tttatgcttg
    21721 gttgacacct tttacagata ttcgtagtgc tcaggagact acaggtggtt cttccgatgt
    21781 tgaatttatg cgaatcaatc aattctacat gcaaacatcg caaaatatgg ccaagtatca
    21841 aggactaaaa acagctggta aggatatcga actcaagtac tttggagttt atgttttgaa
    21901 tgtgacggat aattcaacct ttaaagggat tctcaatatc tctgatacag tcacagcagt
    21961 caatgatcag acctttgata gttccaaaga cttgattgat tacgtcagtt ctcaaaaatt
    22021 aggggattcc gtcaaggtca cctatgaaga ggatgggcaa accaagtctg cagaaggaaa
    22081 gattatcacc ttggaaaatg gcaaaaatgg aattggaatc ggcttgattg accgtacaga
    22141 ggtaatcagc aatgtcccaa ttagcttttc aacagctggt attggcggcc caagtgctgg
    22201 tctcatgttt agtctagcta tctatactca aatagctcac ccagatcttc gtaatggtcg
    22261 tattgttgcc ggtacaggta ccattgaccg cgatggtaat gtgggagaca ttggaggtat
    22321 tgataagaag gttgtagctt cggctagggc aggtgctgct attttctttg ctcctgataa
    22381 ccctgttagc gaagaagaac aaaaggcgca tctggacgcg aaaaacaact accaaacagc
    22441 cctagaagcg gctaaaacaa tcaagacgga tatgaaaatc gtgcccgtta aaaccctaca
    22501 agatgcgatt gattacttga aaaacaatcc ctaatttttg agtgaagttt ccaaactagc
    22561 gcacaaacag agaagatggt ataatagtca aatggttcaa ttattattca ctctaagtag
    22621 tcatatgctc tttatttatg tgagttttta ccttttaaag gatcttgtta gatgggagaa
    22681 ggttttaaaa gtgacagatg ataatacaag aaaagttcgt ttattagtag ccttttttag
    22741 cattgtcata ggctacatcc tgagttcttt ctttattagc ctgtatcatt tgtggcaaga
    22801 agcgcttaga ggattattat aaaatcaaga gtaaaggaaa cgagtatgga taaaattgtg
    22861 gttcaaggtg gcgataatcg tctggtagga agcgtgacga tcgagggagc aaaaaatgca
    22921 gtcttaccct tgttggcagc gactattcta gcaagtgaag gaaagaccgt cttgcagaat
    22981 gttccgattt tgtcggatgt ctttattatg aatcaggtag ttggtggttt gaatgccaag
    23041 gttgactttg atgaggaagc tcatcttgtc aaggtggatg ctactggcga catcactgag
    23101 gaagcccctt acaagtatgt cagcaagatg cgcgcctcca tcgttgtatt agggccaatc
    23161 cttgcccgtg tgggtcatgc caaggtatcc atgccaggtg gttgtacgat tggtagccgt
    23221 cctattgatc ttcatttgaa aggtctggaa gctatggggg ttaagattag tcagacagct
    23281 ggttacatcg aagccaaggc agaacgcttg catggcgctc atatctatat ggactttcca
    23341 agtgttggtg caacgcagaa cttgatgatg gcagcgactc tggctgatgg ggtgacagtg
    23401 attgagaatg ctgcgcgtga gcctgagatt gttgacttag ccattctcct taatgaaatg
    23461 ggagccaagg tcaaaggtgc tggtacagag actataacca ttactggtgt tgagaaactt
    23521 catggtacga ctcacaatgt agtccaagac cgtatcgaag caggaacctt tatggtagct
    23581 gctgccatga ctggtggtga tgtcttgatt cgagacgctg tctgggagca caaccgtccc
    23641 ttgattgcca agttacttga aatgggtgtt gaagtaattg aagaagacga aggaattcgt
    23701 gttcgttctc aactagaaaa tctaaaagct gttcatgtga aaaccttgcc ccacccagga
    23761 tttccaacag atatgcaggc tcaatttaca gccttgatga cagttgcaaa aggcgaatca
    23821 accatggtgg agacagtttt cgaaaatcgt ttccaacacc tagaagagat gcgccgcatg
    23881 ggcttgcatt ctgagattat ccgtgataca gctcgtattg ttggtggaca gcctttgcag
    23941 ggagcagaag ttctttcaac tgaccttcgt gccagtgcag ccttgatttt gacaggtttg
    24001 gtagcacagg gagaaactgt ggtcggtaaa ttggttcact tggatagagg ttactacggt
    24061 ttccatgaga agttggcgca gctaggtgct aagattcagc ggattgaggc aagcgatgaa
    24121 gatgaataag aaatcaagct acgtaatcaa tcgtttactt ttagtcatca tagtactgat
    24181 tttaggtact ctggctctag gaatcggtct aatggtaggt tatggaatct tgggcaaggg
    24241 tcaagatcca tgggctatcc tgtctccagc aaaatggcag gaattgattc ataaatttac
    24301 aggaaattag gctggagaac cagccttttt ctaaagataa ggagaaatat gaacaaaaaa
    24361 acaagacaga cactaatcgg actgctagtg ttattgcttt tgtctacagg gagctattat
    24421 atcaagcaga tgcagtcggc acctaatagt cccaaaacca atcttagtca gaaaaaacaa
    24481 gcgtctgaag ctcctagtca agcattggca gagagtgtct taacagacgc agtcaagagt
    24541 caaataaagg ggagtctgga gtggaatggc tcaggtgctt ttatcgtcaa tggtaataaa
    24601 acaaatctag atgccaaggt ttcaagtaag ccctacgctg acaataaaac aaagacagtg
    24661 ggcaaggaaa ctgttccaac cgtagctaat gccctcttgt ctaaggccac tcgtcagtac
    24721 aagaatcgta aagaaactgg gaatggttca acttcttgga ctcctccagg ttggcatcag
    24781 gtcaagaatc taaagggctc ttatacccat gcagtcgata gaggtcattt gttaggctat
    24841 gccttaatcg gtggtttgga tggttttgat gcctcaacaa gcaatcctaa aaacattgct
    24901 gttcagacag cctgggcaaa tcaggcacaa gccgagtatt cgactggtca aaactactat
    24961 gaaagcaagg tgcgtaaagc cttggaccaa aacaagcgtg tccgttaccg tgtaaccctt
    25021 tactacgctt caaacgagga tttagttccc tcagcttcac agattgaagc caagtcttcg
    25081 gatggagaat tggaattcaa tgttctagtt cccaatgttc aaaagggact tcaactggat
    25141 taccgaactg gagaagtaac tgtaactcag taaaagataa gcctaaactc ctatgtcact
    25201 tatggatgta ggagttcttt ttactagttt aagcagggct agaataggtg ctaagaaaaa
    25261 atagtaactt ctaaaactaa cttccagttt cccacaacct agcttttagt atgagaaaaa
    25321 cgcgtgaaat cagtggaaat ccgtcttaga ctaacttcca gttttgggag agagatggaa
    25381 gttacgctaa gaaaaaaata atatgtattg gattaaattt tgtgatataa taaaggataa
    25441 gatactattt taggagaaga actatggaag acccaagtag tcagaatttg ttgctacaat
    25501 ttgttttatt gtttatcttg acggtattaa atgccttttt ctcagccaca gaaatggcca
    25561 tggtttctct taatcgtgcc cgagttgaac aaaaggcgga agaaggagac agacgctaca
    25621 tccgcctgct gaaggtacta gaaaatccta accacttttt atcaaccatt caggtaggaa
    25681 tcaccctgat tacaatctta tcaggggcga gtttaactga tactctagga cgtgtgattg
    25741 cctcttggct tgggaatggc gaaacagcac aagccgtggc aacttttcta tccttggcat
    25801 ttttgaccta tatttccatc gtttttgggg aattatatcc taagagaatc gctcttaatc
    25861 taaaggatgc cttggcaatt cgtacagcgc cgattatcat tgggatcgga aaactagtca
    25921 gtccttttgt ttggctttta gctgcttcga ccaatttctt gagccatttg actcctatgt
    25981 cattcgatga tgcggatgaa aagatgaccc gtgatgaaat tgcctatatg ctgacaaata
    26041 gtgaagaaac attggatgct gatgagattg agatgctaca aggtgtcttt tcgctcgatg
    26101 aactgatggc acgagaggtt atggtgcctc gaacggatgc ctttatggtg gatattcagg
    26161 atgatagtca agccattatc caaagtattt taaaacaaaa ttattctcgt atcccggttt
    26221 atgatgggga taaggacaat gtaattggaa tcattcacac caagagtctc cttaaggcag
    26281 gctttgtgga cggttttgac aatattgttt ggaagagaat tttacaagat ccactttttg
    26341 tacctgaaac tatttttgtg gatgacttgc taaaagaact gcgaaatacc caaagacaaa
    26401 tggcaatctt gctggatgaa tacggtggta tggctggttt ggtgactttg gaagacctct
    26461 tagaggaaat tgttggtgaa attgatgatg aaacggacaa agcagcaata gatgttcatc
    26521 agattggcga ggatacctac attgttcaag gaactatgac cctcaatgat ttcaataatt
    26581 actttgatgt tgaactggaa agtgatgacg ttgataccat cgctggttat tatttgacag
    26641 gagtgggaac tattccaacg actgagaaac tcagctatga attggtcagc caaaacaaac
    26701 agtttatctt aaccaatgat aaagtgaaga atggacgtgt taccaaggta aaagtccaaa
    26761 tcacagaagt cgaaatagaa gaagaaacag aataaactag aggcttttgt caccaggcgt
    26821 gacgaagctt ttttcaattg atttcaaaag ggaattatgc catggaaaga actctgccac
    26881 aaactcgctc caaaagtttt taaagttata cgtatttatt cgagggaaaa taagaaaagc
    26941 ccttcaaatt gggctttctg ttcatttgaa acctgataaa tcaagttata gaaggcggta
    27001 gacggatttg aaccgacgat caagcttttg cagagccgtg ccttaccact tggctatacc
    27061 gccttaacgt ttattattat accttgaaaa tgatttccag tcaatagttt tttcagtttt
    27121 ttgtaccata gcgtgattcg aatgtgatgt caagttttct atgggaaaaa atttaccaaa
    27181 aacctactga aaccccttga aaaatctagc taaatagggt ataatatggg taatcatttg
    27241 tcgtaggttt tgtctgaaat attgtccaga caaggctcac agcagttaaa tcttctgaaa
    27301 aagtcagatt tagctgctct ttttgtgctt tttttcaaga ttatgagcat ttgtaacaga
    27361 ggcttaaaga ttctgaaaat tcgtcaagag gacacggtga taaggggttt acaaccatat
    27421 gacgattaga aaagcctgat tgacaaggct tggaacttat ttacaaagga gaatcatctt
    27481 ggcaggacat gacgttcaat acgggaaaca tcgtacccgt cgtagttttt caagaatcaa
    27541 agaagttctt gacttaccaa atttgattga aattcaaact gactcattca aagctttcct
    27601 agaccacggt cttaaggaag tgtttgaaga tgtattgcca atttcaaact tcacagacac
    27661 aatggagttg gaatttgttg gatatgaaat caaggaacca aaatacacgc tagaagaagc
    27721 tcgtatccac gatgctagct actcagcacc aatttttgta accttccgtt tgatcaataa
    27781 agaaacaggc gaaatcaaga cccaagaagt tttctttggt gatttcccaa tcatgacaga
    27841 aatgggtact ttcatcatca atggtggtga acgtattatc gtttctcagt tggtccgctc
    27901 accaggtgtt tactttaacg acaaagtaga caaaaatggt aaggtgggct atggttcaac
    27961 tgttatccct aaccgtggag cttggttgga acttgaaagc gactcaaaag atatcaccta
    28021 cactcgtatc gaccgtactc gtaagattcc atttacaacc ttggttcgtg ctcttggttt
    28081 ctcaggtgat gatgaaatct ttgatatctt tggtgacagc gaattggttc gcaacactgt
    28141 tgaaaaagat atccacaaga atccaatgga ctctcgtaca gacgaagcct tgaaagaaat
    28201 ttacgaacgc cttcgtccag gtgagcctaa gacggctgaa agctcacgta gcttgcttgt
    28261 agctcgcttc tttgacccac gtcgttatga cttggcagca gttggtcgtt acaaaatcaa
    28321 taaaaaactc aatgttaaaa cacgtttgct caaccaaacc attgcagagc cattggtaga
    28381 ccctgaaact ggagaaatct tggtagaagc tggtacgatt atgactcgta gcgtgattga
    28441 aagcattgaa agccatttgg atggcgactt gaacaagatt gtctacatcc caaacgatgc
    28501 agccgttgtg actgagcctg ttgttcttca aaaattcaag gttattgctc caactgatcc
    28561 agatcgcgtc gtaacgatca ttggtaatgc taacccagat gacaaggttc gtacggtgac
    28621 tcctgcagat atccttgctg agatgagcta cttcctcaac ttggctgaag gacttggccg
    28681 tgtagatgat atcgaccacc ttggaaatcg tcgtatccgt gcggttggtg aattgcttgc
    28741 caaccaagta cgtttgggac tttctcgtat ggaacgtaat gtccgtgaac gtatgtctgt
    28801 tcaggacaat gaagtcttga caccacaaca aattatcaat attcgtcctg taacagctgc
    28861 agttaaagaa ttctttggtt catcacagtt gtcacagttc atggaccaac acaacccgct
    28921 ttctgagttg tctcacaaac gccgtttgtc agccttagga cctggtggtt tgactcgtga
    28981 ccgtgccgga tatgaagtgc gtgacgtgca ctacactcac tatggtcgta tgtgtccaat
    29041 cgagacacct gaaggaccta acatcggttt gatcaataac ttgtcatctt acggacactt
    29101 gaacaaatat ggttttgttc aaacaccata ccgtaaggtt gaccgtgaaa caggtgttgt
    29161 cacgaacgaa attgtttggt tgacagctga tgaagaagat gaatatactg tagctcaggc
    29221 taactctcgt ctgaatgaag atggaacctt tgctgagaag attgtcatgg gacgtcacca
    29281 aggggtcaac caagagtatc cagctaatat tgttgactac atggacgttt caccaaaaca
    29341 ggtagttgcc gttgcgacag catgtattcc tttcttggaa aacgatgact ccaaccgtgc
    29401 cctcatggga gccaatatgc aacgtcaggc tgtgccattg attaatcctc aggcacctta
    29461 cgttggtact ggtatggaat accaagcagc ccacgattct ggtgcggctg tgattgctca
    29521 gtatgatggt aaagttactt acgcagatgc tgacaaggta gaagttcgtc gtgaagatgg
    29581 ttcattggat gtttaccaca tccaaaaatt ccgtcgttca aactcaggta ctgcttacaa
    29641 ccaacgcact ctcgtaaaag ttggtgatgt cgttgaaaaa ggcgatttca tcgctgacgg
    29701 accttctatg gaaaatggag aaatggcgct tggacaaaac ccaatcgttg cctacatgac
    29761 ttgggaaggc tacaacttcg aggatgccgt tatcatgagc gaacgcttgg tgaaggacga
    29821 tgtctacaca tctgttcacc ttgaagaata cgaatcagaa acgcgcgata caaagcttgg
    29881 gcctgaagaa atcactcgcg aaattccaaa cgttggtgaa gatgccctca aagaccttga
    29941 cgaaatgggg attatccgta ttggtgctga ggttaaagaa ggtgatattc ttgtagggaa
    30001 agtaacacct aagggtgaga aagatctttc agctgaagaa cgtctcttgc acgctatctt
    30061 tggagacaag tctcgtgaag tgcgtgatac ttctcttcgt gtaccacacg gtgccgatgg
    30121 tgtcgttcgt gatgttaaga tctttacacg tgtaaatgga gatgagttgc aatcaggtgt
    30181 taacatgttg gttcgtgttt acatcgctca aaaacgtaag attaaggtcg gagataaaat
    30241 ggccggacgt cacggaaaca aaggggttgt ctctcgtatc gttcctgtag aagacatgcc
    30301 ttaccttcca gacggaactc cagtcgacat catgttgaac ccacttgggg tgccatcacg
    30361 tatgaatatc ggtcaggtta tggagcttca ccttggtatg gcagctcgta ctcttggtat
    30421 tcacattgcg acaccagtct ttgatggagc aagttctgaa gatctttggt caactgttaa
    30481 agaagcaggt atggatagcg atgccaaaac gatcctttac gatggacgta ctggtgaacc
    30541 atttgataac cgtgtttctg ttggagtcat gtacatgatc aaactccacc acatggttga
    30601 cgataaattg cacgcgcgtt cagtcggacc ttactcaact gttacccaac aaccactcgg
    30661 aggtaaagct cagtttggtg gacaacgttt cggtgagatg gaggtttggg ctcttgaagc
    30721 ctacggtgcg tcaaatgtcc ttcaagaaat cttgacttac aagtctgacg atatcaacgg
    30781 acgtttgaaa gcctatgaag ctattacaaa aggcaaacca attccaaaac caggtgttcc
    30841 agaatccttc cgagttcttg ttaaagaatt gcaatctctt ggtcttgaca tgcgtgtcct
    30901 agacgaagat gaccaagaag tggaacttcg cgacttggat gaaggaatgg acgaagatgt
    30961 catccacgta gatgaccttg aaaaagcccg cgaaaaagca gcccaagagg ctaaagcagc
    31021 ctttgaagct gaagaagctg agaaagcaac aaaagcggaa gcaacagaag aagctgctga
    31081 acaagaataa gcagttcact tagaatagaa agggaagaaa tagtggttga tgtaaatcgt
    31141 tttaaaagta tgcaaatcac cctagcttct ccaagtaaag tccgttcatg gtcttatgga
    31201 gaagtcaaaa aacctgaaac aatcaattat cgtaccttga aaccagaacg tgaaggactc
    31261 tttgatgaag tgatctttgg tcctacaaaa gactgggaat gtgcttgtgg taagtacaaa
    31321 cgcattcgtt acagaggaat tgtttgtgac cgctgtgggg ttgaagtaac gcgtacgaaa
    31381 gttcgtcgtg agcgtatggg acatatcgaa ttgaaagctc ctgtatctca catctggtac
    31441 ttcaagggga ttccaagccg tatgggcttg acccttgata tgagccctcg tgccctcgag
    31501 gaagttatct actttgcggc ttatgtggtg attgatccta aggatacacc acttgagcac
    31561 aagtctatca tgacagagcg cgaataccga gagcgcttgc gcgaatatgg ttatggttca
    31621 tttgttgcca agatgggtgc ggaagccatc caagaccttt tgaagcaagt agatcttgaa
    31681 aaagaaattg ctgaactcaa agaagaattg aaaacagcta ctggacaaaa acgtgtcaaa
    31741 gccatccgtc gtttggatgt tttggatgcc ttttacaagt ctggaaacaa acctgaatgg
    31801 atgattctta acatccttcc ggttatccca ccagatcttc gtccaatgtt gcagttggat
    31861 ggtggccgtt ttgcctcatc tgacttgaat gacctttacc gccgtgttat caatcgtaac
    31921 aaccgtttgg ctcgtttgct tgagttaaat gcaccaggta tcatcgttca aaatgagaaa
    31981 cgtatgcttc aagaagcagt tgacgctttg attgacaatg gtcgtcgtgg tcgtccaatc
    32041 acaggaccag gtagccgtcc attgaaatca ttgagccaca tgcttaaagg taaacaagga
    32101 cgtttccgtc aaaacttgct cggtaaacgt gttgacttct caggacgttc agttatcgcc
    32161 gttggtccaa ctcttaagat gtaccaatgt ggtgtgccac gtgaaatggc gattgaactc
    32221 tttaaaccat ttgtcatgcg tgaaatcgtt gcccgtgata tcgtgcaaaa cgtcaaagca
    32281 gctaaacgct tggtggaacg cggagatgag cgtatctggg atatccttga agaagtgatt
    32341 aaagaacacc cagtgctttt gaaccgcgca ccgacccttc accgtttggg tatccaagcc
    32401 ttcgagccag tcttgattga tggtaaggct cttcgcttgc acccacttgt ctgtgaagcc
    32461 tacaatgctg actttgacgg ggaccaaatg gccatccacg taccactttc agaagaagca
    32521 caagcagaag ctcgtatcct catgctagct gctgagcaca tcttgaaccc gaaagatggg
    32581 aaaccggtag ttactccatc tcaggacatg gttttgggta actactactt gaccatggaa
    32641 gaagctggtc gcgaaggtga aggaatggtc ttcaaagacc gtgacgaagc ggttatggct
    32701 taccgcaatg gttatgttca cctccactca cgtgttggta tcgcaacaga cagcctcaac
    32761 aagccttgga cagaagagca aagacataag gtcttgctta caacagttgg taaaattctc
    32821 ttcaacgata tcatgccaga ggggctacca tacttgcaag aaccaaacaa tgccaacttg
    32881 acagaaggtg ttccagctaa atacttcttg ccacttggtg gagatatcaa ggaagctatc
    32941 agcaatcttg agctcaaccc tccattcaag aagaaaaacc tcggaaatat catcgctgaa
    33001 atcttcaaac gtttccgtac gacagaaact tctgccctac ttgaccgcat gaagaacctc
    33061 ggttaccacc actcaactct tgcaggattg acagtgggta ttgccgatat cccagtcgtt
    33121 gatgacaagg ctgaaatcat tgaagaatca cacaaacgtg tcgaacaaat caccaaacaa
    33181 ttccgtcgtg gtatgatcac agacgacgag cgttacaatg ctgttacagc tgaatggcgt
    33241 gctgcccgtg aaaaacttga gaaacgcttg attgctaatc aagatcctaa gaacccaatc
    33301 gttatgatga tggactctgg agcccgtggt aacatctcaa acttctcaca gcttgccggt
    33361 atgcgtggtc tgatggctgc tccgaatgga cgtatcatgg aattgccaat cctttcaaac
    33421 ttccgcgaag gtttgtcggt attggaaatg ttcttctcaa ctcacggtgc tcgtaaaggt
    33481 atgaccgata cggcccttaa gacagccgac tcaggttact tgactcgtcg tttggtcgac
    33541 gttgcccaag acgttatcat ccgtgaggac gactgtggaa ctgaccgtgg tctcttgatc
    33601 cgttctatcg cagaaggaaa agagatgatc gagtctctcg aagagcgtct caacggtcgt
    33661 tacactaaga aaactgttaa acatccagaa actggtgcag tgattattgg tccaaatgaa
    33721 ttgattacag aagacaaggc gcgtgaaatt gtcaatgctg gtgtggaaga agtgactatc
    33781 cgttctgtat ttacatgtaa cactcgtcac ggtgtctgcc gtcactgtta cggtatcaac
    33841 ttggcgactg gtgatgcggt tgaagttggt gaagcagttg gtacaatcgc tgcccaatct
    33901 atcggggaac ctggtacaca gcttacaatg cgtaccttcc acacaggtgg ggttgcctca
    33961 aataccgata tcactcaggg tcttcctcgt gtccaagaaa tctttgaagc ccgcaatcct
    34021 aaaggggaag cggttatcac agaggttaaa ggacaagtta ctgctatcga agaggatgca
    34081 tcaactcgta ccaagaaagt ctttgttaag ggtgaaactg gcgaaggtga atatgtcgtt
    34141 ccatttacag ctcgtatgcg tgtcgaagtt gggggccaag tagcgcgtgg tgctgctctg
    34201 acagaaggtt ctatccaacc aaaacgtctc cttgcagttc gtgatgtctt gtcagttgaa
    34261 acgtaccttc tcggtgaagt acaaaaagtt taccgtagcc aaggggtaga aatcggtgac
    34321 aaacacatcg aggtaatggt tcgtcaaatg atccgtaaag tccgtgtcat ggatccaggt
    34381 gatacagatc ttctcatggg taccctcatg gatatcaatg actttacaga tgccaacaaa
    34441 gatgtcctta tcgcaggtgg agttccagcg acaggtcgcc cagtccttat gggaattacc
    34501 aaagcctcac ttgaaacaaa cagtttcttg tcagcggctt ccttccagga aacaactcgt
    34561 gtccttactg acgcagctat ccgtggtaag aaagaccatc tccttggact taaagaaaat
    34621 gttatcatcg gtaagatcat cccagctggt actggtatgg cccgttaccg taaccttgaa
    34681 ccacatgctg tcaacgaaga agaatacctt aatcctccag tagaggaaga aggaaatgaa
    34741 gaaacaacag aagtagttgt ggatactgcc gttgaaactg tggaagaaac agtagaataa
    34801 gagagaatga gagaaccctc gggttctctc attctctctt ctgaaaactt tctccatttt
    34861 tccacgaaat gtggtaaaat aaaagaaaga agctgacaaa ggagacgggt atggaacaaa
    34921 cattctttat catcaaacca gatggtgtaa aaagagggct agtgggtgaa gtgttaaagc
    34981 gcatcgaaca acgtggattt acaatcgaaa aattggagtt tcgttcacag gtttcagaag
    35041 agttgattga ccagcactat caggacttgg ttggtcagag tttttaccca ccgattcgtg
    35101 aattcatgac ttcaggtcca gttcttgtgg gtgtcatttc tggtcccaaa gtaatcgaaa
    35161 cttggcggac catgatgggt gcaactcgtc cagaagaagc tttaccaggc actattcgag
    35221 gtgattttgc aaaagctgct ggagaaaatg agattatcca aaatgttgta catggttcag
    35281 attcagaaga gtcagctaag cgagaaattg ctctttggtt ttaagagtgg attggctcaa
    35341 tcaattggtt aaaagctcat ttgaatagaa agtatagtca attagtttaa gacatgacgc
    35401 atgatatcaa actttttagt ttttgatatg gtgcgttttt tgattaagtg actattagtt
    35461 tgtctcgctc cgttaggtgc gagacaaaaa aaacacccgc tatgcgggtg cgcgtcgaag
    35521 gttataccaa aaaaactcca aacgcgatac aataaaggtg ttcaatccaa ttgtaaagcg
    35581 aaaggagaaa aatatggcac aaaaggttct tttatcctgt tcttgtttca attgactata
    35641 tttctatact ttttcttcct ttctctgtga aaatcgcaag tttttcccac cttttctcaa
    35701 tactgaaaca acatctttcc agaacttcaa taatttcttt ttgtgttaaa tcattattta
    35761 actaaatctt tggaggcaat acgttttcaa agctactttt ttaccatagg ttttgagcct
    35821 tcaacacaga ttactaagcc aaaaatgctc taaatttttg aaattctaac aaattccact
    35881 caactttatc atcatctttg actttcacaa tatcttcctc aaaataacat ggaataaaga
    35941 tagttttctt ttaaaacggt tgtaggagga aaatctctcc tacactatta ttgattctga
    36001 gagaaaattg caaaatatca tctaccaaac tttcaattat taagattttt tgcagtttat
    36061 ctgaattaga atcgcaaggc ttctcaataa tataattgtc gaattt
//
LOCUS       DAVLWA010000012        35956 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLWA010000012 DAVLWA010000000
VERSION     DAVLWA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.012"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(135..942)
                     /locus_tag="VYN80_000572"
     CDS             complement(join(135..569,571..942))
                     /locus_tag="VYN80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1540525.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIIDATEVKINRPKKELANYSGKKKCYAMKAQAIVTSQGRIVSLDITVN
                     YCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKPLTVEDKTYNH
                     ALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            937..1191
                     /locus_tag="VYN80_000573"
     CDS             937..1191
                     /locus_tag="VYN80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1540526.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1535..2869
                     /gene="trmFO"
                     /locus_tag="VYN80_000574"
     CDS             1535..2869
                     /gene="trmFO"
                     /locus_tag="VYN80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1540527.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2953..3690
                     /locus_tag="VYN80_000575"
     CDS             2953..3690
                     /locus_tag="VYN80_000575"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1540528.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3699..4256
                     /gene="frr"
                     /locus_tag="VYN80_000576"
     CDS             3699..4256
                     /gene="frr"
                     /locus_tag="VYN80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1540529.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4316..5170
                     /gene="cvfB"
                     /locus_tag="VYN80_000577"
     CDS             4316..5170
                     /gene="cvfB"
                     /locus_tag="VYN80_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1540530.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5179..5394
                     /locus_tag="VYN80_000578"
     CDS             5179..5394
                     /locus_tag="VYN80_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1540531.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5480..6448
                     /locus_tag="VYN80_000579"
     CDS             5480..6448
                     /locus_tag="VYN80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1540532.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6426..6605)
                     /locus_tag="VYN80_000580"
     CDS             complement(6426..6605)
                     /locus_tag="VYN80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540533.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6641..7141
                     /locus_tag="VYN80_000581"
     CDS             6641..7141
                     /locus_tag="VYN80_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540534.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVIDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            7144..7404
                     /locus_tag="VYN80_000582"
                     /pseudo
     CDS             7144..7404
                     /locus_tag="VYN80_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7771..8097)
                     /locus_tag="VYN80_000583"
     CDS             complement(7771..8097)
                     /locus_tag="VYN80_000583"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540535.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(8043..8456)
                     /locus_tag="VYN80_000584"
     CDS             complement(8043..8456)
                     /locus_tag="VYN80_000584"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1540536.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8457..8882)
                     /locus_tag="VYN80_000585"
     CDS             complement(8457..8882)
                     /locus_tag="VYN80_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1540537.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            9059..9628
                     /locus_tag="VYN80_000586"
     CDS             9059..9628
                     /locus_tag="VYN80_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540538.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTPETNRAVIELAFEKIGMNKLVALYDKDNSASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9696..10346
                     /locus_tag="VYN80_000587"
     CDS             9696..10346
                     /locus_tag="VYN80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1540539.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10330..12570
                     /locus_tag="VYN80_000588"
     CDS             10330..12570
                     /locus_tag="VYN80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1540540.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPLSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            12712..12936
                     /locus_tag="VYN80_000589"
     CDS             12712..12936
                     /locus_tag="VYN80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540541.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            12968..13555
                     /locus_tag="VYN80_000590"
     CDS             12968..13555
                     /locus_tag="VYN80_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540542.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            13559..14740
                     /locus_tag="VYN80_000591"
     CDS             13559..14740
                     /locus_tag="VYN80_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540543.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVVVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    14900..15031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            15047..15577
                     /gene="infC"
                     /locus_tag="VYN80_000592"
     CDS             15047..15577
                     /gene="infC"
                     /locus_tag="VYN80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1540544.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15610..15810
                     /gene="rpmI"
                     /locus_tag="VYN80_000593"
     CDS             15610..15810
                     /gene="rpmI"
                     /locus_tag="VYN80_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1540545.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15862..16221
                     /gene="rplT"
                     /locus_tag="VYN80_000594"
     CDS             15862..16221
                     /gene="rplT"
                     /locus_tag="VYN80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1540546.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16279..16659
                     /locus_tag="VYN80_000595"
     CDS             16279..16659
                     /locus_tag="VYN80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1540547.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16909..17709
                     /locus_tag="VYN80_000596"
     CDS             16909..17709
                     /locus_tag="VYN80_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1540548.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            17720..18658
                     /locus_tag="VYN80_000597"
     CDS             17720..18658
                     /locus_tag="VYN80_000597"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1540549.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18700..20601
                     /locus_tag="VYN80_000598"
     CDS             18700..20601
                     /locus_tag="VYN80_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1540550.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(20662..22317)
                     /gene="pavA"
                     /locus_tag="VYN80_000599"
     CDS             complement(20662..22317)
                     /gene="pavA"
                     /locus_tag="VYN80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1540551.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22422..22919
                     /gene="ybeY"
                     /locus_tag="VYN80_000600"
     CDS             22422..22919
                     /gene="ybeY"
                     /locus_tag="VYN80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1540552.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22900..23295
                     /locus_tag="VYN80_000601"
     CDS             22900..23295
                     /locus_tag="VYN80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1540553.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVMAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23312..24211
                     /gene="era"
                     /locus_tag="VYN80_000602"
     CDS             23312..24211
                     /gene="era"
                     /locus_tag="VYN80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1540554.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24260..25084
                     /gene="mutM"
                     /locus_tag="VYN80_000603"
     CDS             24260..25084
                     /gene="mutM"
                     /locus_tag="VYN80_000603"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1540555.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25084..25689
                     /locus_tag="VYN80_000604"
     CDS             25084..25689
                     /locus_tag="VYN80_000604"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1540556.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFEQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25676..26875
                     /locus_tag="VYN80_000605"
     CDS             25676..26875
                     /locus_tag="VYN80_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV1540557.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26875..27024
                     /gene="rpmG"
                     /locus_tag="VYN80_000606"
     CDS             26875..27024
                     /gene="rpmG"
                     /locus_tag="VYN80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1540558.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKEKKVTL
                     HLESK"
     gene            27064..27297
                     /gene="secG"
                     /locus_tag="VYN80_000607"
     CDS             27064..27297
                     /gene="secG"
                     /locus_tag="VYN80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1540559.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            27399..29753
                     /gene="rnr"
                     /locus_tag="VYN80_000608"
     CDS             27399..29753
                     /gene="rnr"
                     /locus_tag="VYN80_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1540560.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIVEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGIGRGSNRRSDKKEDKRKS
                     GRSNDKRKYSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29716..30183
                     /gene="smpB"
                     /locus_tag="VYN80_000609"
     CDS             29716..30183
                     /gene="smpB"
                     /locus_tag="VYN80_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1540561.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            30199..31059
                     /gene="tehB"
                     /locus_tag="VYN80_000610"
     CDS             30199..31059
                     /gene="tehB"
                     /locus_tag="VYN80_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1540562.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            31132..32085
                     /gene="coiA"
                     /locus_tag="VYN80_000611"
     CDS             31132..32085
                     /gene="coiA"
                     /locus_tag="VYN80_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1540563.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            32104..33906
                     /gene="pepF"
                     /locus_tag="VYN80_000612"
     CDS             32104..33906
                     /gene="pepF"
                     /locus_tag="VYN80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1540564.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33908..34621
                     /locus_tag="VYN80_000613"
     CDS             33908..34621
                     /locus_tag="VYN80_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1540565.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34688..35629
                     /gene="prsA"
                     /locus_tag="VYN80_000614"
     CDS             34688..35629
                     /gene="prsA"
                     /locus_tag="VYN80_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1540566.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35746..>35956)
                     /locus_tag="VYN80_000615"
     CDS             complement(35746..>35956)
                     /locus_tag="VYN80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEV1540567.1"
                     /translation="KGNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYID
                     YYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT        10811 a         6681 c         8073 g        10391 t
ORIGIN      
        1 gtaagagaga atatagtttt ggtgaaaacg gacttggaac atttcaaaat gtgtttttag
       61 ctgctgaaga tatataggat ttacaaatca taatgaactc acagcttgag aattacatta
      121 gacttcctgc aaaactagaa tcctagttca tgattgataa taccagcaat caaattcatt
      181 cgtaatccga atcgtttacg atgatttcga taggttgttg aaaacatttt aaacgtttct
      241 actttggcaa agatgttctc aaccttgctt ctctccttag atagcgcatg gttataggtt
      301 ttatcttcaa ctgttagcgg cttgaatttg ctggatttac gtggagtttg tgcttgagga
      361 tatatcttca tgagcccttg ataaccactg tcagccaaga ttttaccagc ttgtccgata
      421 tttctgcgac tcattttgaa caacttcata tcatgacaat agttcacagt gatatccaaa
      481 gaaacaattc tcccttgact tgtgacaatc gcttgagcct tcatagcgta gcatttcttt
      541 ttaccagaat aattcgctaa ttcttttttt agggcgattg atttttactt ccgtcgcatc
      601 aataatcatt accgtgtcct cagaactgag aggagttctt gaaatcgtaa caccactttg
      661 aacaagagtt acttcaaccc attggctccg acggattaag ttgctttcgt gaataccaaa
      721 atcagccgca atttgttcat aagttcgata ttctcgcaca tattgaagag tggccataag
      781 aaggtcttct aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc
      841 tgtttttaat acagctaata tctcttcaaa agtcgtgcgc tgaacaccaa caagacgctt
      901 aaatcgtgca tcagttagtt gtttacttgc ttcatcattc atagaactac tataccatat
      961 tttgtttcgc aggaagtcta ttggaaagta agaaatattg aagctgaggc gattagaaga
     1021 aattgtgagc gtggtgctat tttttcaggt aaaataaaac atcacgaaga ttctcagttt
     1081 aaaggagacc actatgttga atgttatgct gttttagata atacggttat agcaagagat
     1141 agaataacag tccctatcga tccgttatgt ggaaaagatt ttatagagta gcatataatt
     1201 gattcttaac tggaatactc tctatctctt tacatcaaga aaaagactaa acagggaagt
     1261 ttgccttctt cccttttttt gttatactag tagaagaaaa aaatagaaag atttgtggga
     1321 gtgaaaaagt ccgggggact ttttcagctt gagccaagaa attcgagagc gaggaaagtc
     1381 tgggggactt tttcagcttg agccaagaaa ttcgagagcg aggaaagtct gggggacttt
     1441 ttcagcctga gccaagaaac tcgaaatctc gtaagtctga ttggcttttt caatgtgaat
     1501 cttaacttca cactcccaaa gaggtattag tgtcgtgtct caatcttata tcaatgttat
     1561 cggtgctggt ttggcaggtt ctgaagcagc ttaccaaatc gcagagcgtg gtattccagt
     1621 taaactttat gaaatgcgtg gtgtcaagtc tacaccccag cataaaacag acaattttgc
     1681 tgagttggtt tgttccaatt ctttgcgtgg ggatgctttg acaaatgcag ttggtcttct
     1741 caaggaagaa atgcgtcgct tgggttctgt tatcttggaa tctgctgagg ctacacgtgt
     1801 tcctgccggt ggagcccttg cggtggatcg tgatggtttc tctcaaatgg tgaccgaaaa
     1861 agtggtcaac caccccttga ttgaagtggt gcgtgatgaa attacagaat tgccaacaga
     1921 tgttattaca gttgtggcga ctggtccctt gactagcgat gccctggctg aaaagattca
     1981 tgcccttaat aatggcgatg gcttctattt ctacgatgca gcagcgccta ttatcgatgt
     2041 caacactatc gatatgagta aggtctatct caaatctcgt tatgataagg gagaagcagc
     2101 ctatctcaat gctccaatga ccaagcaaga gtttatggat ttccatgaag ccttggtcaa
     2161 tgcggaagaa gcaccgctca attcttttga aaaagaaaag tactttgaag gatgtatgcc
     2221 aatcgaagtt atggccaaac gtggcattaa aactatgctt tatggcccta tgaagccagt
     2281 cggtcttgag tatccggacg actacacagg acctcgtgat ggagaattta aaacacctta
     2341 tgcggttgtg caacttcgtc aggataatgc agctggtagc ctatacaata ttgttggttt
     2401 ccagacccac ctcaaatggg gagaacaaaa gcgtgtcttc caaatgattc cgggtcttga
     2461 aaatgcggag tttgtccgtt atggtgtcat gcatcgcaat tcttacatgg attcaccaaa
     2521 tcttcttgag cagacttacc gttccaagaa acaaccaaat ctcttctttg ctggtcagat
     2581 gacgggggtg gaaggctatg ttgagtcggc ggcttcgggc ttagttgcgg gaattaacgc
     2641 agctcgtctc ttcaaggaag aaagcgaggt tattttcccc gagacgacag cgattggaag
     2701 cttagctcat tacattaccc atgccgacag caaacatttc caaccaatga atgtcaattt
     2761 tgggatcatc aaggagttgg aaggcgagcg tatccgtgat aagaaggctc gttatgaaaa
     2821 aattgcagag cgtgcccttg ccgacttaga ggaatttttg actgtctaat ttttttgaaa
     2881 gaattgttca tgatactata aaaatcttag aaattgtgat aaaataggta ggatgaaaga
     2941 aggagagtga aaatggcgaa tcccaagtat aaacgtattt taatcaagtt atcaggtgaa
     3001 gcccttgccg gtgaacgtgg cgtagggatt gatatccaaa cagttcaaac aatcgcaaaa
     3061 gagattcaag aagttcatag cttaggtatc gaaattgccc ttgttattgg tggaggaaat
     3121 ctctggcgtg gagaacctgc agcagaagca ggtatggacc gtgttcaggc agattacact
     3181 ggaatgcttg ggactgttat gaatgctctt gtgatggcag attcattgca acaagttggg
     3241 gttgatacgc gtgtacaaac agctattgct atgcaacaag tggcagagcc ttatgtccgt
     3301 ggacgtgccc ttcgtcacct tgaaaaaggc cgtatcgtta tctttggtgc tggaattggt
     3361 tcaccatact tctcgacaga tacaacagcg gcccttcgtg cagctgaaat cgaagcagat
     3421 gccatcctca tggctaaaaa tggcgtcgat ggtgtgtaca atgccgatcc taagaaggac
     3481 aagacagccg ttaagtttga agaattgacc caccgtgatg ttatcaacaa aggtcttcgt
     3541 atcatggact caacagcctc aaccctctca atggacaacg acattgactt ggttgtcttc
     3601 aacatgaacc aatcaggcaa catcaaacgt gttgtatttg gtgaaaatat cggaacaaca
     3661 gtttcaaata atatcgaaga aaaggaataa gaaagaatat ggctaacgta attattgaaa
     3721 aagctaaaga gagaatgacc cagtctcacc aatcacttgc tcgtgaattt ggtggtatcc
     3781 gtgctggtcg tgccaatgca agcttgcttg accgtgtaca tgtagaatac tatggagtcg
     3841 aaactcctct taaccaaatc gcttcaatta cgattccaga agcgcgtgtt ttgttggtaa
     3901 caccatttga caagtcttca ttgaaagaca tcgaacgtgc cttgaacgct tctgatcttg
     3961 gtatcacacc ggctaatgac ggttctgtga ttcgcttggt tatcccagct cttacagaag
     4021 aaactcgtcg tgaccttgct aaagaagtga agaaggtcgg cgaaaatgct aaagtggctg
     4081 tccgcaatat ccgtcgcgat gctatggacg aagctaagaa gcaagaaaaa gcaaaagaaa
     4141 tcactgaaga cgaattgaag actcttgaaa aagatattca aaaagtaaca gacgatgctg
     4201 ttaaacacat cgacgacatg actgctaaca aagagaaaga acttttggaa gtctaaaaat
     4261 aaacagaaaa actcagttgg cattgctggc tgagttttat tcgaaagaag gaaatatgaa
     4321 tacaaatctt gcaagtttta tcgttggact gatcatcgat gaaaatgacc gtttttactt
     4381 tgtgcaaaag gatggtcaaa cctatgctct tgctaaggaa gagggccaac atacagtagg
     4441 ggatacggtc aaaggttttg catacacgga tatgaagcaa aaactccgcc tgacaacctt
     4501 agaagtgact gccactcagg accaatttgg ttggggacgt gtcacagagg ttcgtaagga
     4561 cttgggtgtc tttgtggata caggccttcc tgacaaggaa atcgttgtgt cactcgatat
     4621 tctccctgtg ctcaaggaac tctggcctaa gaagggcgac caactctaca tccgtcttga
     4681 agtggataag aaagaccgta tctggggcct cttggcttat caagaagact tccaacgtct
     4741 cgctcgtcct gcctacaaca acatgcagaa ccaaaactgg ccagccattg tttaccgtct
     4801 caagctgtca ggaacttttg tttacctacc agaaaataat atgcttggtt ttattcatcc
     4861 tagcgagcgt tacgcagagc cacgtttggg gcaagtatta gatgcgcgcg ttattggttt
     4921 ccgtgaagtg gaccgcactc tgaacctctc cctcaaacca cgctcctttg aaatgttgga
     4981 aaacgatgct cagatgattt tgacttattt ggaaagtaat ggcggtttca tgaccttaaa
     5041 tgataagtca tctccagacg acatcaaggc aacctttggc atttctaaag gtcagttcaa
     5101 gaaagcttta ggtggtctta tgaaggctgg taaaatcaag caggaccagt ttgggacaga
     5161 gttgatttag ggaggcttat gagaaaatca ttttacactt ggctcatgac cgagcgcaat
     5221 cctaaaagta acagtcccaa agcaattttg gcagacctcg cttttgaaga ggcagctttt
     5281 ccaaaacata cagatgattt tgatgaggtc agtcgctttt tggaggagca tgccagtttc
     5341 tcttttaacc taggagattt tgacagcatt tggcaggaat atctagaaca ctagcattta
     5401 ttcattgggt ttgggctagt aatttctcca tccctctgct ataataaaaa gaaataaaag
     5461 gattagagag gttctttatt tgaaggaaca ttcaatagac attcaactga gtcatccaga
     5521 tgacctgttt catctttttg gttccaatga acgccatctt cgtttgatgg aagaagagct
     5581 tgatgttgtg attcatgctc gtacggagat tgtccaggtt ttgggagaag agtctgcctg
     5641 tgaggaagcc cgtcaagtta ttcaggcttt gatggtcttg gtaaatcgtg ggatgaccgt
     5701 tggtacgcca gatgtagtca ctgcgattag catggtcaaa aatgatgaaa ttgacaagtt
     5761 tgtcgccctt tacgaagaag aaattatcaa agataatact gggaaaccta tccgtgtcaa
     5821 aaccctaggg caaaagcttt atgtggacag tgtcaaacag catgatgtga cctttggaat
     5881 tgggccagca ggtacaggga agaccttcct tgcagtgacc ttggcagtga ctgcccttaa
     5941 acgtgggcaa gtcaagcgaa ttatcctaac tcgtccagcg gtggaagcgg gagagagtct
     6001 tggatttctt ccgggtgatc ttaaggagaa ggtggatcct taccttcgtc ctgtttacga
     6061 tgccttgtat cagattctgg ggaaagacca aacaactcga ctcatggagc gtgaaattat
     6121 cgagattgcg ccccttgcct atatgcgtgg acggaccttg gatgatgcct ttgtcattct
     6181 cgatgaggcg caaaatacga ccatcatgca gatgaagatg ttcttgactc gtttaggttt
     6241 tcattctaag atgattgtca atggagatat tagtcagatt gacctgccac gtaatgtcaa
     6301 gtccggtttg attgatgctc aagagaaact caagaacatc catcagattg actttgttca
     6361 tttttcagcc aaggatgtgg ttcgccatcc agttgtcgct cagattatcc gagcctatga
     6421 atattctact gaagttgcac acgactgatt ttgaggaagt ttgcctgcaa aagaatatac
     6481 ttttgagaga ggaaaatcca gttgtatagg ctaaaggttt tatccaaagg tttgagacaa
     6541 cgattaggca cgatggaaag aacttttatg tggctgatga cgatcagtgc atcttcctgt
     6601 gtcataatca cagggcacaa gaaagtagga atttgaaaag atgattgacc aactatctaa
     6661 gtattacagt tgtaggatac taactgaaaa ggatattcca agtattttat ctttatatga
     6721 aagtaatcct ctgtattttc agcattgtcc accagagcca aattttgcaa ctgtaaaaga
     6781 ggacatgctt tgtctacctg aaggtaaagc taaggctgat aagttttttg ttggattttg
     6841 gaatggatct gaccttgtgg ctgttataga ttttgtctat gcacatcctg atgaggagac
     6901 tgtttttatt ggtttgttta tggttgatca agcctatcag aggaaaggga ttggtagtca
     6961 tattgtgaca gaagcactag cttattttgc taagaacttt cgaaaggcac gtttggctta
     7021 tgttaaggga aatccgcaat ctcagcattt ttgggaaaag cagggcttta aatcaattgg
     7081 atgcgaggtt aagcaagaac tctatacggt tgttatcgct gaacagagcc tagaagatta
     7141 gaaatggcat caagtaagaa ctatttggaa tttgttttgg aacaattatc aggattagat
     7201 gatgtgactt accgttccat gattggggag tatattcttt acttccgcgg caagattatt
     7261 ggcggcattt atgacgatcg ctttttagtt aaacccgtgc aagcagtctt agataagatt
     7321 gaccaatctt cttttgagtt tccatacaaa ggtgccaaag aaatgatttg agtggaagaa
     7381 cttgataata agatgtttct ataagacctg attttagcta tgtataacca actgccaacg
     7441 cccaaaccta aaaagaaaaa gcaagggtga acgaagtaaa aaagaagtct gctaaggccc
     7501 tgtctttgca cgggtaaaat tttatatata aaaagaagct gggactaaag agctcagctt
     7561 cctttggttt atataattgt cattacaaga cgaagtggtt gggcgaaact ctgttgactt
     7621 tattcaattt agagtttctt atgcacaatt gagtctggaa cgaaagtctc cagttgcaaa
     7681 gtatacagta caataaacca acgatgtaat agctgatgac acaaagcaca gtgggtagga
     7741 cttgcgaagt cacccttttc ttttcaaaat ttatactaaa tcattgatat cagtgtagtc
     7801 acgattaagt ccttgagcaa ctggtaggct agtcaagtaa ccttgataag tggtcacacc
     7861 ttgacgcaag ccttcatctt cagagattgc ttgtgcgaat cctttgccag ccaaagcttc
     7921 gatataagga agagtgacat tggttagggc gatggttgaa gtgcgggcaa ccgcaccagg
     7981 gatattggca acggcatagt ggagaacacc gtgtttttca tagacgggtt catcgtgcgt
     8041 tgtcacacgg tcagctgttt cgataacgcc accttggtca acagcaacgt caacgataca
     8101 gagcctggac gcatttgttt gaccatctca tctgtcacca attccggtgc ttttgcacca
     8161 gggatgagaa tggctccaat caccacatca gcatctctca tacttgcttc aatgttgaat
     8221 gaattagata taagagtttg aatttgactt ccaaagactt cttctagaac tgagagacgc
     8281 ttggcactaa tatctaaaat agtcacttga gcaccaagac caagggcgat gcgggcagca
     8341 tgtgtaccga cgacaccatc accgatgata gttacttttc cttttggaac acctggtaca
     8401 ccaccaggta gaacaccaga gccaccagct tgcttagtaa ggaagtgagc tccgatttaa
     8461 acagccatac gacctgcaac ctcactcata ggaacgagga gcggtagttg tccttgattg
     8521 tcacgaacag tttcataggc aattcctgtt gtttttgctg ctaacatagc atctgctaat
     8581 tctggagcag cggccatgtg caagtaggtg aagagaagaa gatcgtcgcg caagtaaccg
     8641 tattcagaac ttaaaggttc ttttactttc acaaccaact ctgctgccca agcttcacca
     8701 gcagtagcga caatctcagc tccttgcttt tgatagtcag catcagtaaa gccagaaccg
     8761 agaccagcat ttgtttcgat aaggacacga tgaccacgac taactaagct atgaacacct
     8821 gcaggtgtga gggcgacacg gttttcgtta tttttaattt cttttgggat tccgattaac
     8881 attgagataa cctacctttc aattgacggt cttgttttgg ttgtcacatt ccagttcata
     8941 aatcaaaaat gtgacggttt cattgtatat gaaaccgctt caaaaatcaa gaaaaacttg
     9001 tcatccaaat ttttttatgc tagactagtg aaaatcaagc tctaatggag ggaaaagtat
     9061 ggaatcaata tttgtgaaat ttgcccagta tccgtctata gaaacggagc gtttattgct
     9121 tagacctgta actttggatg atgcggaagc aatgtttgac tatgcctcgg acaagggtaa
     9181 tacacgttac acttttccaa ccaatcaaag cttggaagaa accaagaata acattgctca
     9241 gttctacttg gctaatccct tgggacgttg ggggattgaa ttaaaagata acggcaagtt
     9301 tatcggaact attgacttac acaagattga tcctgttctt aagaaggcag ctattggcta
     9361 cattatcaat aaaaagtatt ggaatcaagg attaacgcca gaaaccaatc gtgctgtgat
     9421 tgagctagct tttgagaaga taggaatgaa taagttagtt gctttatacg ataaggacaa
     9481 ttctgcgtca ggaaaggtca tggagaaatc aggcatgcgt ttttcccatg cagaaccata
     9541 tgcttgtatg gaccagcatg aaaaaggccg aatcgtgaca agagttcatt atgtcttgac
     9601 caaggaagac tattttgcaa ataaataagc agttgaaaag aaatttttcg actgtttttt
     9661 cttcctctta cgaataatct aagagaggag aaaatatgga agcaattatc gagaaaatca
     9721 aagagtataa aatcatcgtc atctgtactg gtctgggctt gcttgtaggc ggatttttcc
     9781 tgctaaagcc agctccacaa acacctgtaa aggaaacgaa tttgcaggct gaagttgcag
     9841 ctgtttccaa ggatttggta tccgaaaagg aagtgaacaa ggaagagaag gaagaacccc
     9901 ttgaacaaga tctaatcaca gtagatgtca aaggtgctgt caaatcgcca gggatttatg
     9961 acttgcctgt aggtagtcga gtcaatgatg ctgttcagaa ggctggtggc ttgacagagc
    10021 aagcagacag caagtcgctc aatctagctc agaaagttag tgatgaggct ctggtttacg
    10081 ttcctactaa gggagaagaa gcagttagtc aacagactgg ttcggggaca gcttcttcaa
    10141 taagcaagga aaagaaggtc aatctcaaca aggccagtct ggaagaactc aagcaggtca
    10201 agggactggg aggaaaacga gctcaggaca ttatcgacca tcgtgaggca aatggcaagt
    10261 tcaagtcagt agacgagctc aagaaggtct ctggcattgg tggcaaaaca atagaaaagc
    10321 ttaaagacta tgttacagtg gattaagaat ttctctattc ccctaattta cctgagtttt
    10381 ctattacttt ggctttatta cgctattttc tcagcatctt atcttgcttt gttgggcttt
    10441 gtttttctgc tagtctgtct ctttatccaa tttccgtgga aatctgctgg taaagttcta
    10501 ataatttgcg gaatctttgg attttggttt gtttttcaaa attggcaaca gagtcaagcg
    10561 agtcaaaatc tggcggattc tgttgaaagg gtacggattt tgcctgatac tattaaggtt
    10621 aatggtgata gtctatcctt tcgtggcaag tctaacggtc gtgctttcca agtctattat
    10681 aaactccagt ccgaggagga gaaagaagcc tttcaagctt taaccgacct gcatgagata
    10741 ggactagaag ggaagctttc ggagccagaa gggcagagaa attttggtgg ctttaattac
    10801 caagcctatc tgaagactca gggaatttac cagactctca atatcaaaaa aatccagtca
    10861 cttcaaaaga ttggcagttg ggatatagga gaaaacttgt ccagtttacg tcgaaaggct
    10921 gtggtttgga ttaagacgca ctttccagac cctatgcgca attacatgac aggactcttg
    10981 ctgggacatc tggacaccga ctttgaggag atgaatgagc tttattccag tctaggaatt
    11041 atccacctct ttgccctatc tggtatgcag gtaggttttt tcatgaatgg atttaagaaa
    11101 cttctcttgc gattgggctt gacccaagaa aagttgaaat ggctgactta tcccttttcc
    11161 cttatctatg cgggactaac tggattttca gcatcggtta ttcgcagtct cttgcaaaag
    11221 ctactggctc aacatggggt taagggcttg gataattttg ccttgacggt gcttgtcctc
    11281 tttattgtca tgccaaactt tttcttgaca gcaggaggag tcttgtcctg cgcttatgct
    11341 tttatcctga ccatgaccag caaagaaggg gaggggctca aggctgttac tagtgaaagt
    11401 ctagtcatct ccttgggcat attgcccatt ctatccttct attttgcgga atttcaacct
    11461 ttgtctatcc ttttgacctt tgtcttttcc tttctatttg acttgacctt cttaccgctc
    11521 ttgtctattt tatttgtcct ttcctttctc tatccagtca ttcagctgaa ctttatcttt
    11581 gaatggttgg agggcattat tcgcttggtg tcacaggtga caagtagacc tctggtcttt
    11641 ggacaaccca atgcatggtt tttaatccta ttgttaattt ccttggcttt ggtctatgat
    11701 ttgagaaaaa acattaaaaa gctaacggta ttgtgcttat tgattacagg gctctttctc
    11761 ctgaccaagc atccactgga aaatgaaatc accatgctgg atgtggggca aggagaaagt
    11821 attttcctac gggatgtaac tgggaaaacc attctcatag atgtaggtgg taaggcagaa
    11881 tcttataaga aaatcgaaaa atggcaagaa aaaatgacga ccagcaatgc ccagcgaacc
    11941 ttgattccct atctcaaaag tcgaggagta gctaagattg accagctaat tttgactaac
    12001 acggacaagg aacatgtcgg agatttgtta gaggtgacca aggctttcca tgtaggggag
    12061 attttagtat caaaaggcag tttgaagcag aagcaatttg tggtagaact acaggcgact
    12121 caaacaaagg tgcgtagtat gacagtaggg gagaacttgc ccatttttgg aagtcagtta
    12181 gaagttctat ccccaaggaa aatgggagat ggaggacacg atgataccct agttctgtat
    12241 gggaaattct tggataagca atttctcttc acgggaaatt tggaggagaa aggagagaag
    12301 gacttgctga agcactatcc agacttgaaa gtaaatgttt tgaaagctag ccaacatggc
    12361 aataaaaaat catcaagtcc agcctttcta gaaaaactca aaccagagct tactcttatc
    12421 tcagttggaa agagcaatcg aatgaaactc ccccatcagg aaacattgac acgactggaa
    12481 ggtatcaata gcaaagttta ccgaactgac cagcaaggag ctatacggtt taaaggttgg
    12541 aatagttgga aaatcgaaag tgttcgatag gaaagataaa tgttgtagat tagtgaaata
    12601 aactaaaaat ttgttgcata ataatgataa aaatggtata atgaaaacgt attcaatact
    12661 gaggatataa aatcattaaa aattagcaaa aatcgtttaa ttagttagtt tatgattatt
    12721 ggtctttttc agtccagtat atctgctgtg acagtcacta aaagttacaa gtatgattgg
    12781 aatacggttt gggaatatag tactaactat cacgaccatc agtatgcttg gattccgtca
    12841 tggtctcgtt attacagcta ttctgagtat ccagttggtt ctggctggaa ctacgctcgt
    12901 tatgaggtca taaactacta tactggaggt tattaatcct taaagagtga gaaaaggagg
    12961 gctggatatg ttaaggctta ctcatgttac cctaaaaacg cgacaagtca tcttgcaaga
    13021 tgcggatttt acctttaaaa agggtagggt ttatggtctt cttgctatca atggctcggg
    13081 aaagacgaca ctattccgag ctatgagcaa gttgcttccc cttagtagtg gacacatcgc
    13141 agttcctcct tctttgtttt attatgagag cgttgaatgg ctggatggaa acttaagtgg
    13201 gatggactac cttcgtctca taaaaaacat ctggaagtca gacctaaact tgagagatga
    13261 aatcgcctac tgggaaatgg ctgactatat cagtcttccc atccgcaagt attccttagg
    13321 catgaagcaa cgcttggtga ttgctatgta ttttctcagt caggccaaat gctggctcat
    13381 ggatgagatt acaaatggct tagacgagta ttatcgacag aagttttttg ataggctagc
    13441 acaaatcgat agacaagaac agctggttct tttaagttcc cactataagg aagagttggt
    13501 tgatatctgc gatagagtag taaccattca tcaggggcag atagaagagg tttagtttat
    13561 gaaagatgtt agtctatttt tattgaaaaa agttttcaaa agtcgtttaa actggattat
    13621 cttactttta tttgcatctg tactcggtgt taccttttat ttaaatagtc agactgcaaa
    13681 ctcacacagc ttggagagca ggttggaaag tcgcattgca gccaacgaga gggctatcaa
    13741 tgaaaatgaa gagaaactct cccaaatgtc tgataccagc tcggaggaat accagtttgc
    13801 taaaaataat ttagacgtgc aaaaaaatct tttgacgcga aagacagaaa ttctaacttt
    13861 actaaaagaa gggcgctgga aagaagccta ctatctgcag tggcaagatg aagagaagaa
    13921 ttatgaattt gtatcaaatg acccgactgc tagctctggc ttaaaaatgg gggttgaccg
    13981 cgaacggaag atttaccaag ccctgtatcc cttgaacata aaagcacata ctttggagtt
    14041 tccgacccac gggattgatc agattgtctg gattttagag gttatcatcc caagcttgtt
    14101 tgtggttgcg attattttta tgctaacaca actatttgca gaaagatatc aaaatcatct
    14161 ggacacagct cacttatatc ctgtttcaaa agtgacattt gcaatatcct ctcttggagt
    14221 tgtagtggga tatgtaactg tgctgtttat cggaatctgt ggcttttctt ttctagtggg
    14281 aagtctgata agtggttttg gacagttaga ttatccctac ccaatttata gcttagtgaa
    14341 tcaagaagta actattggga aaatacaaga tgtattattt cctggcttgc tcttagcttt
    14401 cttagccttt atcgtcattg tggaagtcgt ctacttgatt gcttactttt tcaagcaaaa
    14461 aatgcctgtc ctctttcttt cactcattgg gattgttggc ttattgtttg gcatccaaac
    14521 aattcagcct cttcaaagga ttgcacatct gattcccttt acttacttgc gttcagtgga
    14581 gattttatct ggaagattat ctaagcagat tgataatgtc gatctaaatt ggagcatggg
    14641 gatggtctta cttccttgcc tgattatcct tttgttagtg gggattctat ttatcgaaag
    14701 ctggggaagt tcacgtaaaa aggaagtttt taaagtttag ttttcctata gggaagctaa
    14761 agaaaaacta acatagggag agaatcaact tggttctctc tttttgattc gaaaaccaaa
    14821 ccaaaataca aacacaaact tttcaaaaaa taacttttta tcttgacaag agctagaaaa
    14881 cttggtatca tataaaagtt gagaaaagca gaagtgagag cttctcgcct tgtgacatta
    14941 agttgcctgg ccctacggat gaaaagtttc gaagaaacgc tatcataacg tgcgggcttg
    15001 tatatttaca agtccgctat tgtttttctc taataaaaca aaagaggtga aaaccatagc
    15061 aaagcaagac ttattcatca atgatgagat tcgtgtacgt gaagttcgct tgattggtct
    15121 tgaaggagaa cagctaggta tcaagccact cagtgaagcg caagctttgg ctgataacgc
    15181 taatgttgac ctagtattga ttcaacccca agccaaaccg cctgttgcaa aaattatgga
    15241 ctacggtaag ttcaaatttg agtaccagaa gaagcaaaaa gaacaacgta aaaaacaaag
    15301 cgttgttact gtgaaagaag ttcgtctaag tccaactatt gacaagggtg actttgatac
    15361 aaaacttcgt aatgcacgta agttccttga aaaaggaaat aaagttaagg tatctattcg
    15421 ctttaagggt cgtatgatta cccataaaga gattggtgca aaagttttag ccgagtttgc
    15481 tgaagcaact caagatattg ccatcatcga acaacgtgct aaaatggatg gacgtcaaat
    15541 gttcatgcaa ttggcgccag cgactgacaa aaaataattg tcagaaagtt aaataaagga
    15601 gaaaaaatca tgccaaaaca aaaaacacac cgcgcatcag ctaaacgttt caaacgtaca
    15661 ggttctggtg gacttaaacg tttccgtgct tacacttctc accgtttcca cggaaaaact
    15721 aagaaacaac gtcgtcatct tcgtaaagca tctatggtgc attcaggaga ttacaaacgt
    15781 atcaaagcaa tgcttactcg cttgaaataa tagcttgact gtaagtaaac aattctagga
    15841 aatatttgga ggaaataaaa catggcacgt gttaaaggtg gcgttgtatc acgcaaacgt
    15901 cgtaaacgta ttcttaaatt agcaaaaggt tactatggag ctaaacacat cttgttccgt
    15961 actgcaaaag aacaagtaat gaactcttac tactatgcat accgtgaccg tcgtcagaaa
    16021 aaacgtgact tccgcaaatt gtggattact cgtatcaatg cggcagctcg tatgaacgga
    16081 ctttcatact cacaattgat gcatggtttg aaattggctg agattgaagt taaccgtaaa
    16141 atgcttgctg acttggctgt taacgatgca gtagctttca cagctcttgc agatgcagct
    16201 aaagcaaaac ttggtaaata acaactgatg agactggaac aggattgtcc agtcttttta
    16261 aaaataaagg agaaagatat ggcttcaaaa atgctacaca cttgcttacg agtagaaaat
    16321 cttgaaaaat caatcgcatt ttatcaagat gcttttggtt ttaaagaatt gcgtcgcaga
    16381 gattttccag accatgcctt cacgattgtc tatctaggtc ttgagggtga tgattatgag
    16441 ttggagttga cttataacta cgatcacggt ccgtatgtgg ttggtgatgg gtttgcccat
    16501 atcgccctca gtacacctga tcttgaggca cttcatcaag aacacagtgc aaaaggctat
    16561 gaagtgactg agccaaatgg tctaccagga actacaccaa actattactt tgttaaagac
    16621 cctgatggat ataaggtcga agtcattcgt gaaaaataat ttcgtgcaag ttaagtagaa
    16681 tattcgtttt ctacttgact ttttttagct gaaagagtat actaataaaa attcaacctt
    16741 taaggggagt ccagagagac tcacaaggtg tcagataaaa gaatagtgca attttctaga
    16801 ggagaccttt tgagtgtgct ctcttgtgtt gtacgatttt aactgaggcc ttgcactagc
    16861 aaggtctttt ctttatctgg tccccttaaa atttaaggag gaaaagttat gaatctcaca
    16921 tgtaagaagc gtttgggtgt cattcggttg gaaaccatga aggtggttgc acaagaggaa
    16981 atcgcgccag caatctttga attagtccta gaaggagaaa tggttgaagc catgcgagca
    17041 ggccaatttc ttcatctgcg tgtaccagac gatgcccatc tcttacgtcg tcctatttca
    17101 atttcgtcta ttgacaaggc aaacaagcag tgtcacctca tttatcggat tgagggagct
    17161 gggactgcaa ttttttcaac cttaagtcag ggagacactc ttgatgtgat ggggcctcag
    17221 ggaaatggtt ttgacttgtc tgaccttgat gagcagaatc aggttctcct tgttggtggt
    17281 gggattggtg ttccaccctt gcttgaggtg gccaaggaat tgcatgaacg tggagtgaaa
    17341 gtagtgacag tcctcggttt tgctaataag gatgctgtta ttttgaaaac ggaattggct
    17401 cagtatggtc aggtctttgt aacgacagat gatggttctt atggcatcaa gggaaatgtt
    17461 tccgttgtta tcaatgattt agatagtcag tttgatgctg tttactcgtg tggggctcca
    17521 ggaatgatga agtatatcaa tcaaaccttt gatgatcacc caagagccta tttatctctg
    17581 gaatctcgta tggcttgtgg gatgggagct tgctatgcct gtgttctaaa agtaccagaa
    17641 agcgagacgg tcagccaacg cgtctgtgaa gatggtcctg ttttccgcac aggaacagtt
    17701 gtattataag gagaaaatta tgactacaaa tcgattacaa gtgtctctac ctggtttgga
    17761 tttgaaaaat ccgattattc cagcatcagg ctgttttggc tttggacaag agtatgccaa
    17821 gtactatgat ttagaccttt taggttctat tatgatcaag gcgacaaccc ttgaaccacg
    17881 ttttgggaat ccaactccaa gagtggcaga gacgcctgct ggtatgctca atgcaattgg
    17941 cttgcaaaat cctggtttag aggttgtttt ggctgaaaag ctaccttggc tggaaagaga
    18001 atatccaaat cttcctatta ttgccaatgt agctggtttt tcaaaacaag agtatgcagc
    18061 tgtttctcat gggatttcca aggcaactaa tgtaaaagct atcgagctca atatttcttg
    18121 tcccaatgtt gaccactgta atcatggact tttgattggt caagatccag atttggctta
    18181 tgatgtggtg aaagcagctg tggaagcctc agaagtgcca gtttatgtca aattaacccc
    18241 gagtgtgacc gatatcgtta ctgtcgcaaa agctgcagaa gatgcgggag caagtggctt
    18301 gactatgatc aatactctgg ttggaatgcg ctttgacctc aaaaccagaa aaccaatctt
    18361 ggccaatgga acaggtggaa tgtcaggtcc agcagtcttt ccagtagccc tcaaactcat
    18421 ccgccaagta gcccaaacaa cagacctgcc tatcattgga atggggggag tggattcggc
    18481 tgaagctgcc ctagaaatgt atctggctgg ggcatctgct atcggagttg gaacagctaa
    18541 ctttaccaat ccttatgcct gccctgacat catcgaaaat ttaccaaaag tcatggataa
    18601 atacggtatt agcagtctgg aagaactccg tcaggaagta aaagagtctc tgaggtaaac
    18661 tgcaatcaat ctgttcttga ttttttatta gtttgtaata tgaatttagg agaattttgg
    18721 tacaataaaa taaataagaa cagaggaaga aggttaatga agaaagtaag atttattttt
    18781 ttagctctgc tatttttctt agctagtcca gagggtgcaa tggctagtga tggtacttgg
    18841 caaggaaaac agtatctgaa agaagatggc agtcaagcag cgaatgagtg ggtttttgat
    18901 actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga aaatgaatgg
    18961 ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc caaatcagaa
    19021 tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat gaaacgaaat
    19081 gcttgggtag gaacttccta tgttggtgca acaggtgcca aagtaataga agactgggtc
    19141 tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca cgcagagaaa
    19201 gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta tctactgaca
    19261 agtcagtgga ttaatcaagc ttatgtgaat gctagtggtg ccaaagtaca gcaaggttgg
    19321 ctttttgaca aacaatacca atcttggttt tacataaaag aaaatggaaa ctatgctgat
    19381 aaagaatgga ttttcgagaa tggtcactat tattatctaa aatccggtgg ctacatggca
    19441 gccaatgaat ggatttggga taaggaatct tggttttacc tcaagtttga tggcaaaatg
    19501 gctgaaaaag aatgggtcta cgattctcat agtcaagcct ggtactactt caaatccggt
    19561 ggttacatga cagccaatga atggatttgg gataaggaat cttggtttta tctcaaatct
    19621 gatgggaaaa tagctgaaaa agaatgggtc tacgattctc atagtcaagc ctggtactac
    19681 ttcaaatctg gtggctacat ggcgaaaaat gagacagtag atggttatca gcttggaagc
    19741 gatggtaaat ggcttggagg aaaagctaca aataaaaatg ctgcttacta tcaagtagtg
    19801 cctgtcacag ccaatgttta tgattcagat ggtgaaaagc tttcctatat atcgcaaggt
    19861 agtgtcgtat ggctagataa ggatagaaaa agtgatgaca agcgcttggc tattactatt
    19921 tctggtttgt caggctatat gaaaacagaa gatttacaag cgctagatgc tagtaaggac
    19981 tttatccctt attatgagag tgatggccac cgtttttatc actatgtggc tcagaatgct
    20041 agtatcccag tagcttctca tctttctgat atggaagtag gcaagaaata ttattcggca
    20101 gatggcctgc attttgatgg ttttaagctt gagaatccct tccttttcaa agatttaaca
    20161 gaggctacaa actacagtgc tgaagaattg gataaggtat ttagtttgct aaacattaat
    20221 aatagccttt tggagaacaa gggcgctact tttaaggaag ccgaagaaca ttatcatatc
    20281 aatgcccttt atctccttgc ccatagtgcc ctagaaagta actggggaag aagtaaaatt
    20341 gccaaagata agaataattt ctttggcatt acagcctatg atacgacccc ttacctttct
    20401 gccaagacat ttgatgatgt ggataaggga attttaggtg caaccaagtg gattaaggaa
    20461 aattatatcg ataggggaag aactttcctt ggaaacaagg cttctggtat gaatgtggaa
    20521 tatgcttcag acccttattg gggcgaaaaa attgctagtg tgatgatgaa aatcaatgag
    20581 aagctaggtg gcaaagatta gtactataag tgaatatgat ttgagtgaat agtaagttaa
    20641 aaatcctgat ttcaagtaaa atcaggattt tttcatggat gcaatttttt tggagtctgg
    20701 tgtgacgcgg agggtctttt gtcctgtgta agtgacaaag ccgggttttc caccagttgg
    20761 tttattgagt tttttgactt caatcatatc tacctgcacc agattcgaca ggcgcccttg
    20821 agagaagtag gcagctaact ctgctgcgtc tgtcttgact gcatcagatg ggtcaagatt
    20881 tcctgagatg acaacatggc ttccaggaat gtccttagca tggaaccaaa gttcctcctt
    20941 gcgggccatt ttaaaggtca attcctcatt ttgaagattg tttcgtccga catagatgat
    21001 ggttttgcca tcgcttgcta gatattgttc tagttttttg cgtttctgga ttttctcccg
    21061 ttgtcttctg cggataaaac ctgtttgaat caattcttca cggatttcag cgatttcttc
    21121 cagtccagct tggttgagga cggtttctac actttccaga tagagaatag tggctttggt
    21181 ttcttcaatc aaatcagtca agtatttgac agcttctttg agtttctgat accgtttaaa
    21241 atagcgttgg gcattctggt tgggagtcag agccttatca agcgcaatca tgataggttg
    21301 gttggtatag tagttgtcta ggataacctg gtcttggtcg ttaggcactt ggtgtaggaa
    21361 ggttgtcagc aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg ccagtaactc
    21421 tttttcctgt tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa cacgacgaat
    21481 cagttcactg gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat agtaggtgtc
    21541 caacaaatca gaaagatttg caaaaggctc tcccacctga tttgcaaaag gaactggact
    21601 gaaggaagtc tcagtcaagc atggcttggt ttcttgattg aaaaaatttc ggaaagcgga
    21661 aagtttttca ctaaccagta tcctttccaa ttcatttgca gtatcgcgtc ccagaccttg
    21721 aaagaggctt tgaagatttt ttgctgttag ttcttgggtt tgcaggattt caaagagctt
    21781 ttcatctttg atagtaaaag gattgagaga ttctgtactt ggcggagcga tataggtcga
    21841 tcctggaagt aaggtgcggt agctattttg tgaaaaaccg acgtgtttga taacttcgag
    21901 gattttatga ctgcttttat cgaccagtag aatattactg tgtttcccca taatttcaat
    21961 aatcaaggta gcctggatat ggtctccaat ctcgttttta ttggaaactg taatttccac
    22021 aatacggtca ttttccactt gctcaatcga ctcaatcagg gccccctgca aatactttct
    22081 caaaaccatg ataaaggtag aaggttgagc tggattttca aaagtcgttt gggtcagctg
    22141 aatgcgtcca aaaactggat gggcagaaag gagcaggcga tggctttggc gattgctgcg
    22201 gatttgcaag accaactctt gttcaaaagg ctgattgatt ttctggatgc gaccattcac
    22261 taattcgctt cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa atgacatcgt
    22321 tctctccttg tgattgtatt ccatagtatt atatcaaaaa ggtagaataa aatcatggaa
    22381 atgtggtata ataaagccaa gtaaagagaa acgagaagca catgtatatt gaaatggtag
    22441 atgaaactgg tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt ttggaatttg
    22501 cagcccaaaa attaggaaaa gaagacaagg agatggcagt cacttttgtg accaatgagc
    22561 gtagtcatga acttaatctg gagtaccgtg acaccgaccg tccgacagat gtcatcagcc
    22621 ttgagtataa accagaattg gaaattgcct ttgacgaaga ggatttgctt gaaaatccag
    22681 aattggcaga gatgatgtct gagtttgatg cctatattgg ggaattgttc atctctatcg
    22741 ataaggctca tgagcaggcc gaagaatatg gtcacagctt tgagcgtgag atgggcttct
    22801 tggcagtaca cggcttttta catattaacg gctatgatca ctatactccg gaagaagaag
    22861 cggagatgtt cggtttacaa gaagaaattt tgacagccta tggactcaca agacaataaa
    22921 cgaaaatgga aaaatcgtga cttgatatcc agtttagaat ttgccatcac agggattttt
    22981 actgctatca aggaagaacg aaatatgcga aaacatgcag tgatggctct agtggtcatc
    23041 cttgcaggtt ttgtttttca ggtgtcacga atcgaatggc tctttcttct attgagtatt
    23101 ttcttggtag tagcctttga gattatcaac tctgctattg aaaatgtggt ggatttggcc
    23161 agtcactatc acttttccat gctggctaaa aatgccaagg atatggcggc cggcgcggta
    23221 ttagtggttt ctctttttgc agccttaaca ggcgcattga tttttctccc acgaatctgg
    23281 gatttattat tttaaacagt aagaggaaat tatgactttt aaatcaggct ttgtagccat
    23341 tttaggacgt cccaatgttg ggaagtcaac ctttttaaat cacgttatgg ggcaaaagat
    23401 tgccatcatg agtgacaagg cgcagacaac gcgcaataaa atcatgggaa tttacacgac
    23461 tgataaggag caaattgtct ttatcgacac accagggatt cacaaaccta aaacagctct
    23521 cggagatttc atggttgagt ctgcctacag tacccttcgc gaagtggata ccgttctttt
    23581 catggtgcct gctgatgaag cgcgtggtaa gggggacgat atgattatcg agcgtctcaa
    23641 ggctgccaag gttcctgtga ttttggtggt gaataaaatc gataaggtcc atccagacca
    23701 gctcttgtct cagattgatg acttccgtaa tcaaatggac tttaaggaaa ttgttccaat
    23761 ctcagccctt cagggaaata acgtgtctcg tctagtggat attttgagtg aaaatctgga
    23821 tgaaggtttc caatatttcc cgtctgatca aatcacagat catccagaac gtttcttagt
    23881 ttcagaaatg gttcgcgaga aagtcttgca cctaactcgt gaagagattc cgcattctgt
    23941 agcagtagtt gttgactcta tgaaacgaga cgaagagaca gacaaggttc acatccgtgc
    24001 aaccatcatg gtcgagcgcg atagccaaaa agggattatc atcggtaaag gtggcgctat
    24061 gcttaagaaa atcggtagta tggcccgtcg tgatatcgaa ctcatgctag gagacaaggt
    24121 cttcctagaa acctgggtca aggtcaagaa aaactggcgc gataaaaagc tagatttggc
    24181 tgactttggc tataatgaaa gagaatacta agtagaggta ggctcatgcc tgcttcttgt
    24241 ttttacagaa ggaggactta tgcctgaatt acctgaggtt gaaaccgttt gtcgtggctt
    24301 agaaaaattg attataggaa agaagatttc gagtatagaa attcgctacc ccaagatgat
    24361 taagacggat ttggaagagt ttcaaaggga attgcctagt cagattatcg agtcaatggg
    24421 acgtcgtgga aaatatttgc ttttttatct gacagacaag gtcttgattt cccatttgcg
    24481 gatggagggc aagtattttt actatccaga ccaaggacct gaacgcaagc atgcccatgt
    24541 tttctttcat tttgaagatg gtggcacgct tgtttatgag gatgttcgca agtttggaac
    24601 catggaactc ttggtgcctg accttttaga cgcctacttt atttctaaaa aattaggtcc
    24661 tgaaccaagc gaacaagact ttgatttaca ggtctttcaa tctgcccttg ccaagtccaa
    24721 aaagcctatc aaatcccatc tcctagacca gaccttggta gctggacttg gcaatattta
    24781 tgtggatgag gttctctggc gagctcaggt tcatccagct agatcttccc agactttgac
    24841 agcagaagaa gcgacagcta ttcatgacca aaccattgct gttttgagcc aggctgttga
    24901 aaaaggcggt tccaccattc ggacttatac caatgccttt ggggaagatg gaagcatgca
    24961 ggactttcat caggtctatg ataagactgg tcaagagtgt gtacgctgtg gtaccatcat
    25021 tgagaaaatt caactaggcg gacgaggcac ccacttttgt ccaaactgtc aaaggaggga
    25081 ctgatgagaa aaatcatcgg aatcactggg ggaattgcct ctggtaagtc aactgtgaca
    25141 aattttctaa gacagcaagg ctttcaagta gtggatgccg acgcagtcgt ccaccaacta
    25201 cagaaacctg gtggtcgtct gtttgaggct ctagtacagc actttgagca agaaatcatt
    25261 cttgaaaacg gagaactcaa tcgccctctc ctagctagtc tcatcttttc aaatcctgaa
    25321 gagcaaaaat ggtcgaagca aattcaaggg gagattatcc gtgaggaact ggctactttg
    25381 agagaacagt tggctcagac agaagagatt ttcttcatgg atattcccct actttttgag
    25441 caggactaca gcgattggtt tgctgagact tggttggtct atgtggaccg agatgcccaa
    25501 gtagaacgct taatgaaaag ggaccagttg tccaaagatg aagctgagtc tcgtctggca
    25561 gcccagtggc ctttagaaaa aaagaaagat ttggccagcc aggttcttga taataatggc
    25621 aatcagaacc agcttcttaa tcaagtgcat atccttcttg agggaggtag gcaagatgac
    25681 agagattaac tggaaggata atctgcgtat tgcctggttt ggtaattttc tgacaggagc
    25741 cagtatttct ttggttgtac cttttatgcc catcttcgtg gaaaatctag gtgtagggag
    25801 tcagcaagtc gctttttatg caggcttagc aatttctgtc tctgctattt ccgcggcgct
    25861 cttttctcct atttggggta ttcttgctga caaatacggc cgaaaaccca tgatgattcg
    25921 ggcaggtctt gctatgacta tcactatggg aggcttggcc tttgtcccaa atatctattg
    25981 gttaatcttt cttcgtttac taaacggtgt atttgcaggt tttgttccta atgcaacggc
    26041 actgatagcc agtcaggttc caaaggagaa atcaggctct gccttaggta ctttgtctac
    26101 aggcgtagtt gcaggtactc taactggtcc ctttattggt ggctttatcg cagaattatt
    26161 tggcattcgt acagttttct tactggttgg tagttttcta tttttagctg ctattttgac
    26221 tatttgcttt atcaaggaag attttcaacc agtagccaag gaaaaggcta ttccaacaaa
    26281 ggaattattt acctcggtta aatatcccta tcttttgctc aatctctttt taaccagttt
    26341 tgtcatacaa ttttcagccc aatcgatcgg ccctattttg gctctttatg tacgcgactt
    26401 agggcagaca gagaatcttc tttttgtctc tggtttgatt gtgtccagta tgggcttttc
    26461 cagcatgatg agtgcaggag tcatgggcaa gctaggtgac aaggtgggca atcatcgtct
    26521 cttggttgtc gcccagtttt attcagtcat catctatctc ctctgtgcca atgcctctag
    26581 cccccttcaa ctaggactct atcgtttcct ctttggattg ggaaccggtg ccttgattcc
    26641 cggggttaat gccctactca gcaaaatgac tcccaaagcc ggcatttcga gggtctttgc
    26701 cttcaatcag gtattctttt atctgggagg tgttgttggt cccatggcag gttctgcagt
    26761 agcaggtcaa tttggctacc atgctgtctt ttatgcgacg agcctttgtg ttgcctttag
    26821 ttgtctcttt aacctgattc aatttcgaac attattaaaa gtaaaggaaa tctagtgcga
    26881 gtaaaaatca atctcaaatg ctcctcttgt ggcagtatca attacctaac cagtaaaaat
    26941 tcaaaaaccc atccagacaa gattgaggtt ttaaagtatt gtcccaagga aaaaaaagta
    27001 accctacatc ttgaatctaa gtagatttta tggtaaaata atagggattt taaggagttt
    27061 gatatgtata acctattatt aaccatttta ttagtattat ctgttgtgat tgtgattgca
    27121 attttcatgc aaccaaccaa aaaccaatcc agcaatgtat ttgatgccag ttcaggtgat
    27181 ttgtttgaac gcagtaaagc tcgcggtttt gaagctgtaa tgcagcgttt gacagggatt
    27241 ttagtctttt tctggctagc cattgcctta gcattgacgg tattatcaag tagataagaa
    27301 aataatgggc aggactaggt ctttgcctct ttttattttt aaaggatgtt tgagaaggtt
    27361 ttacagtaaa agaaaattaa aaaatctaga aagaaaatat gaaagataga ataaaagaat
    27421 atttacaaga caagggaaag gtgactgtta atgatttggc tcaggctttg ggaaaagaca
    27481 gttccaagga ttttcgtgag ttgattaaaa ccttgtcctt aatggaaaga aagcaccaaa
    27541 ttcgttttga agaagatggt agtctgacat tagaaattaa gaaaaaacat gagattaccc
    27601 tcaaggggat ttttcatgcc cataaaaatg gctttggctt tgttagtctg gaaggcgagg
    27661 aggacgacct ttttgtaggg aaaaatgatg tcaactatgc tattgatggt gataccgtcg
    27721 aggtagtgat taagaaagtc gctgaccgca ataagggaac agcagcagaa gccaaaatta
    27781 ttgatatcct agaacacagt ttgacaacag ttgtcgggca aatcgttctg gatcaggaaa
    27841 aacctaagta tgctggctat attcgttcaa aaaatcagaa aatcagtcaa ccgatttatg
    27901 ttaagaaacc agccctaaaa ttagaaggaa cagaagttct caaggtcttt atcgataaat
    27961 acccaagcaa gaaacatgat ttctttgtcg cgagtgttct cgatgtggtg ggacactcaa
    28021 cggatgtcgg aattgatgtt cttgaggttt tggaatcaat ggacattgta tccgagtttc
    28081 cagaagctgt tgttaaggaa gcagaaagtg tgcctgatgc tccgtctcaa aaggatatgg
    28141 aaggtcgtct ggatctaaga gatgaaatta cctttaccat tgacggtgcg gatgccaagg
    28201 acttggacga tgcagtgcat atcaaggctc tgaaaaatgg caatctggag cttggggttc
    28261 acatcgcaga tgtttcttat tatgtgaccg aggggtctgc ccttgacaag gaagccctta
    28321 accgtgcgac ttctgtttac gtgacagacc gagtggtgcc aatgcttcca gaacgactat
    28381 caaatggcat ctgctctctc aatccccaag ttgaccgcct gacccagtct gctattatgg
    28441 agattgataa acatggtcgt gtggtcaact ataccattac acaaacagtt atcaagacca
    28501 gttttcgtat gacctatagc gatgtcaatg atatcctagc tggcgatgaa gaaaagagaa
    28561 aagaatatca taaaattgta ccaagtatcg aactcatggc caagcttcat gaaactttag
    28621 aaaacatgcg tgtgaaacgt ggagctctca attttgatac caatgaagcg aagattttag
    28681 tggataaaca aggtaagcct gttgatatcg ttcttcggca gcgtggcatt gccgagcgga
    28741 tgattgagtc ttttatgttg atggctaatg aaacagttgc cgaacatttc agcaagttgg
    28801 atttgccttt tatctatcga attcacgagg agcctaaggc tgaaaaggtt cagaagttta
    28861 ttgattatgc ttcgagtttt ggcttgcgca tttatggaac tgccagtgag attagtcagg
    28921 aggcacttca agacatcatg cgtgctgttg agggagaacc ttatgcagat gtattgtcca
    28981 tgatgcttct tcgctctatg cagcaggctc gttattcgga gcacaatcac ggccactatg
    29041 gactagctgc tgactattat actcacttta ccagtccaat tcgtcgttat ccagaccttc
    29101 ttgttcaccg tatgattcgg gattacggcc gttctaagga aatagtagag cattttgagc
    29161 aagtgattcc agagattgcg acccagtctt ccaaccgtga acgtcgtgcc atagaagctg
    29221 agcgtgaagt cgaagccatg aaaaaggctg agtatatgga agaatacgtg ggtgaagagt
    29281 atgatgcagt tgtatcaagt attgtcaaat tcggtctctt tgtcgaattg ccaaacacag
    29341 ttgaaggctt gattcacatc actaatctac ctgaatttta tcatttcaat gagcgtgatt
    29401 tgactcttcg tggagaaaaa tcaggtatca ctttccgagt gggtcagcag atccgtatcc
    29461 gtgttgaaag agcggataaa atgactggag agattgattt ttcattcgta cctagtgagt
    29521 ttgatgtgat tgaaaaaggc ttgaaacagt ctagtcgtag tggcataggg cgtggttcca
    29581 atcgtcgttc ggataagaag gaagacaaga gaaaatcagg acgctcaaat gataagcgta
    29641 agtattcaca aaaagacaag aagaaaaaag gaaagaaacc tttttacaag gaagtagcta
    29701 agaaaggagc caagcatggc aaagggcgag ggaaaggtcg tcgcacaaaa taaaaaggca
    29761 cgccacgact atacaatcgt agatacgcta gaggcaggga tggtcctgac tggaactgaa
    29821 atcaagagtg tacgagctgc tcgaattaat ctcaaggatg gctttgctca agtgaaaaat
    29881 ggagaagttt ggctgagcaa tgttcatatc gcgccttacg aagagggcaa tatctggaac
    29941 caggaaccag aacgtcgtcg taaactcctg ctccataaaa agcaaattca aaaattggaa
    30001 caagagacca aagggacagg aatgacctta gttcccctta aggtctatat aaaagatggc
    30061 tacgctaagc ttcttttagg acttgccaaa gggaagcatg actatgacaa acgggagtct
    30121 atcaaacgtc gtgagcaaaa tcgagatatc gcgcgtgtga tgaaagctgt taatcagcga
    30181 taaaaagagg aattgaaaat ggaaaaatta gttgcctata aacgcatgcc tttgtggaat
    30241 aaacaaacaa tgcctgaagc tgttcagcaa aagcacaata caaaagttgg gacttggggg
    30301 aaaattactg tcttgaaggg agctctcaag tttattgaat tgacagaaga aggggaagtt
    30361 ctagctgaac acctctttga agcaggggca gacaatccaa tggcccaacc tcaagcctgg
    30421 caccgagtgg aagctgccac agatgatgtg gaatggtact tggaatttta ttgtaaacct
    30481 gaggattatt ttgctaaaaa atacaatacc aatcctgttc attcagaggt cctagaggcc
    30541 atgcagacag tgaaacaagg gaaagctttg gatttgggtt gtggtcaggg gcgtaattct
    30601 ctttttctag cccagcaaga ttttgatgtg acggctgtag atcaaaatgg actagctctt
    30661 gaaatcttgc aaagcattgt ggagcaggaa gatttggaca tgcctgttgg cctttacgat
    30721 atcaattcag ctagcattga acaagaatat gattttatcg tttcaacagt tgttctcatg
    30781 tttctacaag cggaccgcat tccagctatt attcaaaata tgcaggagaa aaccagtgtt
    30841 ggtggttaca accttatcgt ttgtgccatg gacacggagg attatccttg ctcggttaac
    30901 ttcccattca cctttaaaga aggagaactg gcagactatt acaaggattg ggaattggtt
    30961 aagtacaatg aaaatccagg ccatttgcac cgtcgcgatg agaatggcaa tcgtattcaa
    31021 ctacgctttg cgaccttact agctaagaaa atcaagtaaa catacatgaa gattaggaat
    31081 tttcctgatc ttttttcttt tttacgaatg atatagaaaa ggagggaatt catgtttgtt
    31141 gcgagagatg ctaggggaga attggtaaat gtgttagagg ataaacttga gaagcaagca
    31201 tacacctgcc cagcttgtgg aggccagatc catttgcgtc aaggaccaag tgtacggacg
    31261 cattttgccc ataaatcctt aaaagactgt gattttttct ttgaaaatga aagtccagaa
    31321 cacctggcca ataaggaatc cctctatcac tggttgaaaa aagagacaaa ggttcaatta
    31381 gagtacccgc tttcagaact taaacagatt gcggatgtat ttgtaaatgg caatctagct
    31441 ctagaagttc agtgtagtcc cttgcctcag aaagtcctta aagagcgaag tgagggctat
    31501 cgtagtcagg gttaccaagt actgtggttg ctgggtcaaa aactgtggct caaggagcgt
    31561 ttgactcgtc tacagcaagg ttttctttat ttcagtcaaa acatgggctt ttatgtttgg
    31621 gaattagaca aggaaaaaca agttttaaga ctcaaatacc tgatttacca ggatctccgc
    31681 ggtaaactcc attatcaaat caaggaattt tcctatggtc aaggtagttt attggaaata
    31741 ttgcgtcttc cctataagag acaaaaaata tctcatttta cagtttctga ggacaaggac
    31801 atctgtcgct atatccggca acaactttat tatcaaaatc tcttttggat gaaagaacaa
    31861 gcagaagcct atcaaaaggg agaaaatatc ctgacttatg gactgaaaga atggtatcca
    31921 caaattcgac caatagtggg caaatttttc cagattgaac aagacttgac tagctattat
    31981 cagcactttt atacctatta ccaaaaaaat cctcaaaatg attggcaaaa gctttatcca
    32041 ccagcctttt atcagcaata tttcttgaaa aatatggtag aatagaaagg atggaggaat
    32101 ctaatggtat tacaaagaaa tgaaataaat gaaaaagata catgggatct atcaacgatc
    32161 tacccaactg accaggcttg ggaagaagcc ttaaaagatt taacagaaca attggagaca
    32221 gtagcccagt atgaaggcca tctcttggat agtgcggata acctactaga aatcactgaa
    32281 ttttctcttg aaatggaacg ccagatagag aagctttacg tttatgctca tatgaagaat
    32341 gaccaggata cacgtgaagc taagtatcaa gagtactatg ccaaggccat gacactctac
    32401 agccagttag accaagcctt ttcattctat gagcctgaat ttatggagat tagcgaaaag
    32461 cagtatgctg actttttaga agctcaacca aagctgcagg tttatcaaca ctattttgac
    32521 aagctcttgc aaggcaagga tcacgttctt tcacaacgtg aagaagaatt attggctgga
    32581 gctggagaaa tctttggttc agcaagtgaa accttcgcta tcttggacaa tgcggatatt
    32641 gtgttccctt atgtcctaga cgatgatggt aaagaagttc agctatctca tgggacttac
    32701 acacgtttga tggagtctaa aaaacgtgag gttcgccgtg gtgcctatca agctctttat
    32761 gcgacttacg aacaattcca acacacctat gccaaaacct tgcaaaccaa tgttaaggtg
    32821 caaaattacc gtgctaaagt tcgtaactac aagagtgctc gtcatgcagc cctcgcagcg
    32881 aattttgttc cagaaagtgt ttatgacaat ttggtagcag cagttcgcaa gcatttgcca
    32941 ctcttacatc gctatcttga gcttcgttca aaaatcttgg ggatttcaga tctcaagatg
    33001 tacgatgtct acacaccgct ttcatctgtt gaatacagtt ttacctacca agaagccttg
    33061 aaaaaagcag aagatgcttt ggcagtcttg ggtgaggatt acttgagccg tgttaaacgt
    33121 gccttcagcg agcgttggat tgatgtttac gaaaatcaag gcaagcgttc aggtgcctac
    33181 tctggtggtt cttacgatac caatgccttt atgcttctca actggcagga caatctggac
    33241 aatctcttta ctcttgttca tgaaacaggt cacagtatgc attcaagcta tactcgtgaa
    33301 actcagcctt atgtttacgg ggattactct atctttttgg ctgagattgc ctcaactacc
    33361 aatgaaaata tcttgacgga gaaattattg gaagaagtgg aagacgacgc aacacgcttt
    33421 gctattctca ataacttcct agatggtttc cgtggaacag ttttccgcca aactcaattt
    33481 gctgagtttg aacacgccat tcaccaagca gatcaaaatg gggaggtctt gacaagcgat
    33541 ttcctaaata aactctacgc agacttgaac caagagtatt atggtttgag taaggaagac
    33601 aatcctgaaa tccaatacga gtgggctcgc attccacact tctactataa ctactatgta
    33661 tatcaatatt caactggctt tgcggccgcc tcagccttgg ctgaaaaaat tgtccatggt
    33721 agtcaagaag accgtgaccg ctatatcgac tacctcaagg caggtaagtc ggactatcca
    33781 cttaatgtca tgagaaaagc tggtgttgat atggagaagg aagactacct caacgatgcc
    33841 tttgcagtct ttgaacgccg tttaaatgag tttgaagccc ttgttgaaaa attaggattg
    33901 gcataaaatg gttgaatcgt atagtaagaa tgctaaccat aacatgcgtc gtcctgtcgt
    33961 caaagaagaa attgtagact tgatgcgtca gcgtcaaaag caggtcacag gtttcttgaa
    34021 agaattggaa gactttgccc gcaaggaaaa tattcctatt attccccatg aaacggttgc
    34081 ttatttccgt tttcttatgg aaaccatgca gcctaaaaat attctggaaa ttgggacggc
    34141 tatcggtttt tcagctctct tgatggctga acatgcgcca aatgctaaga ttacaactat
    34201 tgatcgtaat ccagaaatga ttggttttgc caaggaaaat tttgcccagt ttgacagtcg
    34261 caagcaaatc actctcctag agggagatgc ggtggatgtc ttatctacac tgacagagtc
    34321 ttatgatttc gtatttatgg attctgccaa gtctaaatac atcgtctttc tgccagaaat
    34381 cctcaaacat ttggaagttg gtggtgtggt tgtcttggat gatatttttc aaggtggtga
    34441 tgttgccaag gatattatgg aagtccgtcg tggtcagcga accatttatc gaggccttca
    34501 aaaattattt gatgcaacct tagacaatcc agaactcacc gcaacattag tgcctttagg
    34561 agatggtatt ctcatgcttc gtaaaaatgt agcagatgtt caactgtctg aaagcgaatg
    34621 attttcagaa aaatttaaga aaaaatagta aaatagatag agtaacactt atctcaaagg
    34681 agtagacatg aagaaaaaat tattggcagg tgccatcaca ctattatcag tagcaacttt
    34741 agcagcttgt tcgaaagggt cagaaggagc agaccttatc agcatgaaag gggatgtcat
    34801 cacagaacat caattttatg agcaagtgaa aaacaaccct tcagcccaac aagtcttgtt
    34861 aaatatgacc atccaaaaag tttttgaaaa acaatatggc tcagagcttg atgataaaga
    34921 ggttgatgat actattgccg aagaaaaaaa acaatatggc gaaaactacc aacgtgtctt
    34981 gtcacaagca ggtatgactc ttgaaacacg taaagctcaa attcgtacaa gtaaattagt
    35041 tgagttggca gttaagaagg tagcagaagc tgaattgaca gatgaagcct ataagaaagc
    35101 ctttgatgag tacactccag atgtaacggc tcaaatcatc cgtcttaata atgaagataa
    35161 ggccaaagaa gttctcgaaa aagccaaggc agaaggtgct gattttgctc aattagccaa
    35221 agataattca actgatgaaa aaacaaaaga aaatggtgga gaaattacct ttgattctgc
    35281 ttcaacagaa gtacctgagc aagtcaaaaa agccgctttc gctttagatg tggatggtgt
    35341 ttctgatgtg attacagcaa ctggcacaca agcctacagt agccaatatt acattgtaaa
    35401 actcactaag aaaacagaaa aatcatctaa tattgatgac tacaaagaaa aattaaaaac
    35461 tgttatcttg actcaaaaac aaaatgattc aacatttgtt caaagcatta tcggaaaaga
    35521 attgcaagca gccaatatca aggttaagga ccaagccttc caaaatatct ttacccaata
    35581 tatcggtggt ggagattcaa gctcaagcag tagtacatca aacgaatagt ccaaatcaat
    35641 gagtcaggga aaaaactcta cttcaggaaa aaatgaagca aacattccca caataaaacg
    35701 cgtagtacaa ggtttgtact gacccccaaa agttagacaa ataatttatc cgaaggattt
    35761 agttctgtat tgcacagggc taagtccttt tagttttacc ttaattctct tgttgttgta
    35821 ataatcaata tagtctataa tggcttgttc caattgatta agtgatttaa atgttttctc
    35881 atagccataa aacatttcgg attttaaaat accaaagaaa gattccatcc taccgttgtc
    35941 ttggctgttt cccttg
//
LOCUS       DAVLWA010000013        34344 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLWA010000013 DAVLWA010000000
VERSION     DAVLWA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.013"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            508..960
                     /locus_tag="VYN80_000616"
     CDS             508..960
                     /locus_tag="VYN80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1540568.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGEVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            979..2316
                     /locus_tag="VYN80_000617"
     CDS             979..2316
                     /locus_tag="VYN80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1540569.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2395..3279
                     /locus_tag="VYN80_000618"
     CDS             2395..3279
                     /locus_tag="VYN80_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1540570.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3279..4112
                     /locus_tag="VYN80_000619"
     CDS             3279..4112
                     /locus_tag="VYN80_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1540571.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4131..6224
                     /gene="nanB"
                     /locus_tag="VYN80_000620"
     CDS             4131..6224
                     /gene="nanB"
                     /locus_tag="VYN80_000620"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1540572.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6237..7340
                     /locus_tag="VYN80_000621"
     CDS             6237..7340
                     /locus_tag="VYN80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1540573.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7514..8212
                     /locus_tag="VYN80_000622"
     CDS             7514..8212
                     /locus_tag="VYN80_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1540574.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8231..9748
                     /locus_tag="VYN80_000623"
     CDS             8231..9748
                     /locus_tag="VYN80_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1540575.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9873..11201
                     /locus_tag="VYN80_000624"
     CDS             9873..11201
                     /locus_tag="VYN80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540576.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11453..12319
                     /locus_tag="VYN80_000625"
     CDS             11453..12319
                     /locus_tag="VYN80_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1540577.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12335..13174
                     /locus_tag="VYN80_000626"
     CDS             12335..13174
                     /locus_tag="VYN80_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1540578.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13446..13898
                     /locus_tag="VYN80_000627"
     CDS             13446..13898
                     /locus_tag="VYN80_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1540579.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14040..14348
                     /locus_tag="VYN80_000628"
     CDS             14040..14348
                     /locus_tag="VYN80_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540580.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LSRAAATAAARVGLSTKISGWILRVAVNVADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14358..14573
                     /locus_tag="VYN80_000629"
     CDS             14358..14573
                     /locus_tag="VYN80_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540581.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDEHTWSTILIASLFGV
                     YDYKPIDKNRKSPKEKRKNRFVHGRDL"
     gene            14631..15275
                     /locus_tag="VYN80_000630"
     CDS             14631..15275
                     /locus_tag="VYN80_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1540582.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            15448..16365
                     /locus_tag="VYN80_000631"
     CDS             15448..16365
                     /locus_tag="VYN80_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1540583.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEILKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVIEEDRPVVEAAAALIRETKERFL"
     gene            16383..17267
                     /locus_tag="VYN80_000632"
     CDS             16383..17267
                     /locus_tag="VYN80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1540584.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17283..18134)
                     /locus_tag="VYN80_000633"
     CDS             complement(17283..18134)
                     /locus_tag="VYN80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1540585.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18334..20391
                     /gene="pbp2b"
                     /locus_tag="VYN80_000634"
     CDS             18334..20391
                     /gene="pbp2b"
                     /locus_tag="VYN80_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1540586.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKELP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20402..20998
                     /gene="recR"
                     /locus_tag="VYN80_000635"
     CDS             20402..20998
                     /gene="recR"
                     /locus_tag="VYN80_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1540587.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21174..22217
                     /locus_tag="VYN80_000636"
     CDS             21174..22217
                     /locus_tag="VYN80_000636"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1540588.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPGLIERLVDL
                     AKESFDKREAHLI"
     gene            22301..23674
                     /locus_tag="VYN80_000637"
     CDS             22301..23674
                     /locus_tag="VYN80_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1540589.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGQDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23661..24272
                     /locus_tag="VYN80_000638"
     CDS             23661..24272
                     /locus_tag="VYN80_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1540590.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24363..25067
                     /locus_tag="VYN80_000639"
     CDS             24363..25067
                     /locus_tag="VYN80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1540591.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25286..26659
                     /gene="ftsA"
                     /locus_tag="VYN80_000640"
     CDS             25286..26659
                     /gene="ftsA"
                     /locus_tag="VYN80_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1540592.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26676..27935
                     /gene="ftsZ"
                     /locus_tag="VYN80_000641"
     CDS             26676..27935
                     /gene="ftsZ"
                     /locus_tag="VYN80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1540593.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27940..28611
                     /locus_tag="VYN80_000642"
     CDS             27940..28611
                     /locus_tag="VYN80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1540594.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28621..29160
                     /locus_tag="VYN80_000643"
     CDS             28621..29160
                     /locus_tag="VYN80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1540595.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29160..29423
                     /locus_tag="VYN80_000644"
     CDS             29160..29423
                     /locus_tag="VYN80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1540596.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29420..30205
                     /locus_tag="VYN80_000645"
     CDS             29420..30205
                     /locus_tag="VYN80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1540597.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLSPDYFQPEFSDFEISLQEIVYSNKFEYL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30214..31002
                     /gene="divIVA"
                     /locus_tag="VYN80_000646"
     CDS             30214..31002
                     /gene="divIVA"
                     /locus_tag="VYN80_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1540598.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31350..34142
                     /locus_tag="VYN80_000647"
     CDS             31350..34142
                     /locus_tag="VYN80_000647"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1540599.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPVGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     ICFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10540 a         6073 c         7486 g        10245 t
ORIGIN      
        1 ttggtgaaac gagatgtaga aaggagattt agccaaagag tctattagtg ctagaataat
       61 agattagaat tattttagaa aaacgaagtg agcagcttat aaattcaagt ccccaaatag
      121 attcatacta gtatcttttg caaaaaataa agggcgattt ccttcatgaa tatcaatttc
      181 atctataagg aaggtagcta attgaactaa cttatttatt ctgtttgtcg ctagaaaaat
      241 cagacctcct tgtgaagatt gaggagatac tgaatgaaaa tcaaagaaga aactagcaag
      301 ctagtagcag attgcccaaa acaccgcttt gaggttgtag ataagactga cgtatataat
      361 ccaaggtgaa gcgactgtgg tttgaagaga ttttcaaaga gtataggcta gagagtagtg
      421 tttttatgtc cttctagtag aaaatgctag acagaagaat ggggaacttg gataggaaaa
      481 atagattgag aaaggaggtt agaagagatg attattacaa aaattagccg tttaggaact
      541 tatgtgggag taaatccaca ttttgcaaca ttaatagatt ttttagaaaa aacaggacta
      601 gaaaatttaa cagaaggttc gattgctatc gatggtaatc gattgtttgg gaattgcttt
      661 acttatctag cagatggtca agcaggagcc ttctttgaaa cccaccaaaa atatttggat
      721 attcatttag tgctggaaaa cgaagaagcc atggctgtga cgtcgccgga aaatgtaagc
      781 gttacccaag agtatgatga agagaaggat attgaattat acacagggga agtggaacag
      841 ttagttcatt tgagagctgg cgaatgcctc atcacttttc cagaagattt acatcaaccc
      901 aaggttcgta taaatgatga acctgtgaaa aaagttgtct ttaaagttgc gatttcttaa
      961 tgtagaaaga gaagaacgat gaaaaaaatg agaaagtttt tatgtctagc tggaattgcg
     1021 ctagcggctg ttgccttggt agcttgttca ggaaaaaaag aagctacaac tagtactgaa
     1081 ccaccaacag aattatctgg tgagattaca atgtggcact cctttactca aggaccccgt
     1141 ttagaaagta ttcaaaaatc agcagatgct ttcatgcaaa agcatccaaa aacgaaaatc
     1201 aagattgaaa cattttcttg gaatgacttc tatactaaat ggactacagg tttagcaaat
     1261 ggaaatgtgc cagatatcag tacagctctt cctaaccaag taatggaaat ggtcaactca
     1321 gatgctttgg ttccgctaaa tgattctatc aagcgtattg gacaagataa atttaacgaa
     1381 actgccttaa atgaagcaaa aatcggagat gattactact ctgttcctct ttattcacat
     1441 gcacaagtca tgtgggttag aacagatttg ttaaaagaac ataatattga ggttcctaaa
     1501 acttgggatc aactctatga agcttctaaa aaattgaaag aagctggagt ttatggcttg
     1561 tctgttccgt ttggaacaaa tgacttaatg gcaacacgtt tcttgaactt ctacgtacgt
     1621 agtggtggag gaagcctctt aacaaaagat cttaaagcag acttgacaag ccaacttgct
     1681 caagatggta ttaaatactg ggttaaattg tataaagaaa tctcacctca agattctttg
     1741 aactttaatg tccttcaaca agctaccttg ttctatcaag gaaaaacagc atttgacttt
     1801 aactctggct tccatatcgg aggaattaat gccaacagtc ctcaattgat tgattcgatt
     1861 gatgcttatc ctattccaaa aatcaaagag tctgataaag accaaggaat tgaaacctca
     1921 aacattccaa tggttgtttg gaaaaattca aaacatccag aagttgctaa agcattctta
     1981 gaagcacttt ataatgaaga agactacgtt aaattccttg attcaactcc agtaggtatg
     2041 ttgccaacta ttaaggggat tagcgattct gcagcctata aagaaaatga aactcgtaag
     2101 aagtttaaac atgctgaaga agtaattact gaagctgtta aaaaaggtac tgctattggt
     2161 tatgaaaatg gaccaagtgt acaagctggt atgttgacta accaacacat tattgaacaa
     2221 atgttccaag atatcattac aaatggaaca gatcctatga aagcagcaaa agaagcagaa
     2281 aaacaattaa atgatttatt tgaggctgtt cagtagatgt aaaagactag aaaataggtg
     2341 ggctagtgag ctgaaaagct ctagcccaat cttgtaaaag aagggagaag gagaatggtt
     2401 aaagaacgta atttaactcg ctggatattt gttttgccag ctatgattat cgtaggatta
     2461 ctctttgttt atccgttttt ctcgagtatt ttttatagct ttaccaataa gcatttgatt
     2521 atgcctaatt ataaatttgt tggtttggct aactataaag ctgtgctatc agatcccaac
     2581 ttctttaatg cgttctttaa ttcaattaag tggaccgttt tctcattagt tggtcaagtt
     2641 ttagtagggt ttgtattggc tttagctctt cacagagtac gccacttcaa gaaattatat
     2701 aggaccttat tgattgttcc ttgggcattt cctaccatcg ttattgcctt ttcttggcag
     2761 tggattctaa acggggttta tggctactta cctaatctaa tcgtaaaatt aggtttaatg
     2821 gaacatacac ctgcattttt gacagatagt acatgggcat tcctatgttt ggtgtttatc
     2881 aacatttggt ttggagcacc aatgattatg gttaatgtgc tttcagcttt gcaaacagta
     2941 ccagaagaac aatttgaggc tgctaagata gatggtgctt caagttggca ggtgttcaag
     3001 tttatcgtct ttccacatat taaagtggtt gtaggacttc tagttgtttt gagaactgta
     3061 tggatcttta ataactttga cattatctac ctcattactg gtggtggacc agccaatgct
     3121 acaacgacgc ttccaatttt tgcttacaac ctgggttggg gaactaaatt gttgggtcgt
     3181 gcttcagcag ttacagtatt gctctttatc ttcttggtgg cgatttgctt tatctacttt
     3241 gctatcatca gtaagtggga aaaggagggt agaaaataat gaagaagaaa tccagtattt
     3301 atttagatat tctctcacat gtacttttag ttggtgcgac catcgttgca attttcccat
     3361 tggtatggat tatcatatct tctgtcaaag ggaaagggga attaactcag tatccaacac
     3421 gattttggcc tgaacagttt acattagatt atttcactca tgttatcaac gatttgcact
     3481 tcattgataa cattcgaaac agtttaatca ttgccttggc tacaaccctc attgcgatta
     3541 ttatttctgc tatggcagcc tatggtattg ttcgattctt tcctaaattg ggagcaatca
     3601 tgtcgagact actcgtcatt acctatattt tcccaccaat tttgttagca attccctatt
     3661 caattgccat tgctaaagtt gggttaacaa atagtttatt tggcttgatg atggtttatc
     3721 tatcttttag tgttccatat gcagtttggc tcttagttgg atttttccaa acagttccaa
     3781 ttggaattga agaagcggct agaattgatg gtgcaaataa atttgttacg ttttataaag
     3841 ttgtgctacc gattgtagca ccaggtattg tagcaacagc tatttataca tttatcaatg
     3901 cttggaatga attcctgtat gccttgattt tgattaacaa tacaggaaag atgacagtag
     3961 cagtagccct tcgttcactt aatggttcag aaatactaga ctggggagat atgatggcag
     4021 cgtctgttat tgtagttctt ccatcaatta ttttcttctc tatcatccaa aataagattg
     4081 caagtggatt atcagaagga tctgtgaagt agacgaaaga aggaaaaaaa atgaataaaa
     4141 gaggtcttta ttcaaaacta ggaatttctg ttgtaggcat tagtctttta atgggagttc
     4201 ccactttgat tcatgcgaat gaattaaact atggtcaact gtccatatct cctatttttc
     4261 aaggaggttc atatcaactg aacaataaga gtatagatat cagctctttg ttattagata
     4321 aattgtctgg agagagtcag acagtagtaa tgaaatttaa agcagatgaa ccaaactctc
     4381 ttcaagcttt gtttggccta tctaatagta aagcaggctt taaaaataat tacttttcaa
     4441 ttttcatgag agattctggt gagataggtg tagaaataag agacgcccaa aagggaataa
     4501 attatttatt ttctaggcca gcttcattat ggggaaagca taaaggacag gcagttgaaa
     4561 atacactagt atttgtatct gattctaaag ataaaacata cacaatgtat gttaatggaa
     4621 tagaagtgtt ctctgaaaca gttgatacat ttttgccaat ttcaaatata aatggtatag
     4681 ataaggcaac actaggagct gttaatcgtg aaggtaagga acattacctc gcaaaaggaa
     4741 gtattgatga aatcagtcta tttaacaaag caattagtga tcaggaagtt tcaaatattc
     4801 ccttgtcaaa tccatttcag ttaattttcc aatcaggaga ttctactcaa gctaactatt
     4861 ttagaatacc gacactatat acattaagta gtggaagagt tctatcaagt attgatgcac
     4921 gttatggtgg gactcatgat tctaaaagta agattaatat tgccacttct tatagtgatg
     4981 ataatgggaa aacgtggagt gagccaattt ttgctatgaa gtttaatgac tatgaggagc
     5041 agttagttta ctggccacga gataataaat taaagaatag tcaaattagt ggaagtgctt
     5101 cattcataga ttcatccatt gttgaagata aaaaatctgg gaaaacgata ttactagctg
     5161 atgttatgcc tgcgggtatt ggaaataata atgcaaataa agccgactca ggttttaaag
     5221 aaataaatgg tcattattat ttaaaactaa agaagaatgg agataacgat ttccgttata
     5281 cagttagaga aaatggtgtc gtttatgatg aaacaactaa taaacctaca aattatacta
     5341 taaatgataa gtatgaagtt ttggagggag gaaagtcttt aacagtcgaa caatattcgg
     5401 ttgattttga tagtggctct ttaagagaaa aacataatgg aaaacaggtc cctatgaatg
     5461 ttttctacaa agattcgtta tttaaagtga ctcctactaa ttatatagca atgacaacta
     5521 gtcagaatag aggagagagt tgggaacaat ttaagttgtt gcctccgttc ttaggagaaa
     5581 aacataatgg aacttactta tgtcccggac aaggtttagc attaaaatca agtaacagat
     5641 tgatttttgc aacatatact agtggagaac taacctatct catttctgat gatagtggtc
     5701 aaacatggaa gaaatcctca gcttcaattc cgtttaaaaa tgcaacagca gaagcacaaa
     5761 tggttgaact gagagatggt gtgattagaa cattctttag aaccactaca ggtaagatag
     5821 cttatatgac tagtagagat tctggagaaa catggtcgaa agtttcgtat attgatggaa
     5881 tccaacaaac ttcatatggc acacaagtat ctgcaattaa atactctcaa ttaattgatg
     5941 gaaaagaagc agtcattttg agtacaccaa attctagaag tggccgtaag ggaggtcaat
     6001 tagttgttgg tttagtcaat aaagaagatg atagtattga ttggaaatac cactatgata
     6061 ttgatttgcc ttcgtatggt tatgcctatt ctgcgattac agaattgcca aatcatcaca
     6121 taggtgtgct gtttgaaaaa tatgattcgt ggtcgagaaa tgaattgcat ttaagcaatg
     6181 tagttcagta tatagatttg gaaattaatg atttaacaaa ataaaggaga aaaaacatgg
     6241 ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc tacatgcaaa
     6301 agaatgatgg agcagagatt actcttctct atgatccaga taatgcagag gcgattgcag
     6361 aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc gatgaagtag
     6421 attgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt aaggctgcac
     6481 agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa gattgtcgcg
     6541 agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat attatgaatt
     6601 tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc ggagacgttc
     6661 tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta tcatggaaaa
     6721 aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg gattgcgttc
     6781 aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat gtggcccatg
     6841 aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa ttttctaata
     6901 agcgttttgc cttgttagaa tggggttcag cttatcgttg gggtgaacat tatgtcttaa
     6961 tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga actcttaagc
     7021 tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat gatgatcgga
     7081 ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa ccaggtaaac
     7141 gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg catgagatta
     7201 tggaaggagc cccagtatca gaagaatttg caaaactttt gacaggtgaa gctgccctag
     7261 aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc aaagtaaaat
     7321 tgtcagaaat tgtaaaataa attttggtat tctcctattt ataggtcgac ttgctcctct
     7381 gaaagtactt ttagaggagc tgtttgactt tgctagtttt tgaaactgaa atctattata
     7441 ctacaaacta ttgaaagcgt tttaatttta aggtataata atctcataga aataaagaaa
     7501 aggaggaaag aggatgccac agattagcaa agaagccttg attgagcaaa tcaaagatgg
     7561 aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt tatacagaag cgggaggggt
     7621 gattcccttg ctggtcaaag cggctgagca aggtggagca gtcggtatcc gagcaaacag
     7681 tgttcgcgat atcaaggaaa ttaaggaagt cactaaactt ccaatcattg ggattatcaa
     7741 acgtgattat ccacctcagg aacccttcat cacggctact atgaaagaag ttgatgaatt
     7801 ggcagaactg gacatcgagg tgattgctct ggattgtacc aagcgtgaac gctacgatgg
     7861 tttggaaatt caagagttca ttcgtcaggt taaggagaaa tatcctaatc agcttttgat
     7921 ggctgatact agtatcttcg aagaagggct agcagctgta gaagcaggaa ttgactttgt
     7981 cggaacaacc ttatcaggct acacatccta cagtccaaaa gtagacggtc cagattttga
     8041 attgattaag aaactctgtg atgccggtgt agatgtcatt gcagaaggaa aaattcatac
     8101 accagaacaa gccaaacaaa tccttgaata tggagtgcga ggcatcgttg ttggtggcgc
     8161 cattactaga ccaaaagaga ttacagaacg cttcgttgct agtcttaaat aagatgtgag
     8221 agggagtttt atgtttaaag ttttacaaaa agttggaaaa gcttttatgt tacctatagc
     8281 tatacttcct gcagcaggtc tacttttggg gattggtggt gcactttcaa acccaaccac
     8341 gatagcaact tatccaatac tagacaatag tatttttcaa tcaatattcc aagtaatgag
     8401 ctctgcagga gaggttgtat tcagtaattt gtcactactt ctctgtgtgg gattatgtat
     8461 tggcttagcg aaacgagata aaggaaccgc tgcgttagca ggagtaactg gttacttagt
     8521 tatgactgca acgatcaaag ctttggtaaa actttttatg gcagaaggat ctgcaattga
     8581 tactggagtt attggagcat tagttgtcgg aatagttgcc gtatatttgc acaaccgata
     8641 taacaatatt caattacctt ccgctttagg attctttgga ggttcacgct tcgttcctat
     8701 tgttacatcg ttctcttcta tcttgattgg ctttgtcttc tttgttattt ggccaccttt
     8761 ccaacaactt cttgtttcta caggtggata tatttctcag gcgggtccaa ttggaacttt
     8821 tctatatgga tttttaatga gactttctgg agcagtaggc ttacatcata taatttaccc
     8881 tatgttttgg tatactgaac ttggtggtgt tgaaactgtt gcaggacaaa cagtggttgg
     8941 agctcaaaaa atattttttg ctcaattagc cgatccggcc cattctggat tatttacaga
     9001 aggaacaagg ttttttgcag gtcgtttctc aacaatgatg ttcggtttac cggctgcctg
     9061 tttagcgatg taccatagtg ttcctaaaaa tcgtcgtaaa aaatacgcgg gtttgttttt
     9121 tggagttgct ttaacatctt ttattaccgg tattacagaa ccaattgaat ttatgtttct
     9181 attcgtcagt ccggttctat atgttgttca cgcattcctt gatggtgtta gcttctttat
     9241 tgcagacgtc ttaaatattt caataggaaa cacattttca ggaggtgtaa tcgatttcac
     9301 tttatttgga attttgcagg ggaacgctaa gacgaattgg gttcttcaga ttccaattgg
     9361 acttatttgg agtgttttgt attatattat ttttagatgg ttcattactc aattcaacgt
     9421 tctaacgcca ggacgaggag aagaagtaga ttctaaagaa atttctgaat ccgcagattc
     9481 aacttcaaat actgcagatt atttaaaaca ggatagccta caaattatca gagccttggg
     9541 tggatcaaat aatatagaag atgtagatgc ttgtgtgaca cgtttacgtg tagctgtaaa
     9601 agaagttaat caagttgata aagcactttt aaaacaaatt ggtgcagttg atgtcttaga
     9661 agtgaagggt ggcattcaag caatctatgg agcaaaagca atcttatata aaaatagtat
     9721 taatgaaatt ttaggtgtag atgattaagt acttactgac ttaataaaaa acagaggaga
     9781 gtgatggatg agtaggatga aatgaaatcg tatacaagaa ataaagaact cattatccaa
     9841 gttggatacg cttattacat aggagaatac aaatgaaatt tagaaaatta gcttgtacag
     9901 tacttgcggg tgctgcggtt cttggtcttg ctgcttgtgg caattctggc ggaagtaaag
     9961 atgctgccaa atcaggtggt gacggtgcca aaacagaaat cacttggtgg gcattcccag
    10021 tatttaccca agaaaaaact ggtgacggtg ttggaactta tgaaaaatca atcatcgaag
    10081 cgtttgaaaa agcaaaccca gatataaaag tgaaattgga aaccatcgac ttcaagtcag
    10141 gtcctgaaaa aatcacaaca gccatcgaag caggaacagc tccagacgta ctctttgatg
    10201 caccaggacg tatcatccaa tacggtaaaa acggtaaatt ggctgagttg aacgacctct
    10261 tcacagatga atttgttaaa gatgtcaaca atgaaaacat cgtacaagca agtaaagctg
    10321 gagacaaggc ttatatgtat ccgattagtt ctgccccatt ctacatggca atgaacaaga
    10381 aaatgttaga agatgctgga gtagcaaacc ttgtaaaaga aggttggaca actgatgatt
    10441 ttgaaaaagt attgaaagca cttaaagaca agggttacac accaggttca ttgttcagtt
    10501 ctggtcaagg gggagaccaa ggaacacgtg cctttatctc taacctttat agcggttctg
    10561 taacagatga aaaagttagc aaatatacaa ctgatgatcc taaattcgtc aaaggtcttg
    10621 aaaaagcaac tagctggatt aaagacaatt tgatcaataa tggttcacaa tttgacggtg
    10681 gggcagatat ccaaaacttt gccaacggtc aaacatctta cacaatcctt tgggcaccag
    10741 ctcaaaatgg tatccaagct aaacttttag aagcaagtaa ggtagaagtg gtagaagtac
    10801 cattcccatc agacgaaggt aagccagctc ttgaatacct tgtaaacggg tttgcagtat
    10861 tcaacaataa agacgacaag aaagtcgctg catctaagaa attcatccag tttatcgcag
    10921 atgacaagga gtggggacct aaagacgtag ttcgtacagg tgctttccca gtccgtactt
    10981 catttggaaa actttatgaa gacaaacgca tggaaacaat cagcggctgg actcaatact
    11041 actcaccata ctacaacact attgatggat ttgctgaaat gagaacactt tggttcccaa
    11101 tgttgcaatc tgtatcaaat ggtgacgaaa aaccagcgga tgctttgaaa gccttcactg
    11161 aaaaagcaaa cgaaacaatc aaaaaagcta tgaaacaata gttcttagtt attctataaa
    11221 aagtagtttt ttaaagaacc taagagtgta tacccccttt tccctctaca cagatagtgt
    11281 aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa atgaagttct
    11341 tacataagcg aatcataaaa aatttcattt tgattttaaa acagttcaag aaagtcaaaa
    11401 aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc gtatgcggga
    11461 aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca tctttgtgtt
    11521 ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga ctaaatttga
    11581 gtttgtaggc ttggataact atatccgtat gtttaaagat cctgtcttta caaaatctct
    11641 gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat tctcactctt
    11701 tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc gtttcgtctt
    11761 cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat ggatttatga
    11821 cccactatca gggattctaa actttgtcct taagtccagc cacatcatca gccaaaacat
    11881 ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc tcttgaccac
    11941 ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg acaattcact
    12001 ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga agattaaatg
    12061 gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta actcattcca
    12121 gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa caagtacctt
    12181 gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg ccaacacaat
    12241 tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta aagtacttgg
    12301 aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa aaaaaccatt
    12361 aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc tgttcatctt
    12421 tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa ttgttattcc
    12481 tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca tggtgcagaa
    12541 ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca tgttcttagt
    12601 ttgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct atggtcaacg
    12661 cattctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg tccttgtacc
    12721 attggtacgt atcgtcaact tcatgggaat ccacgatact ctctgggcag ttatcttgcc
    12781 tttgattgga tggccattcg gtgtcttcct catgaaacaa tttagtgaaa atattcctac
    12841 agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct tctggagtgt
    12901 agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct tcatcaatac
    12961 ttggaatgac tacttcatgc aattggtaat gttgacttca cgtaacaatt tgaccatctc
    13021 acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga ttatggcagg
    13081 agctgccctt gctgctgttc caatcgtcac agtcttcctt gtcttccaaa aatccttcac
    13141 acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatct cttcaaacta
    13201 cgtcagcttc accttgccat acttaagtat tgcctgcggt tagcttccta gtttgttctt
    13261 caattttcat tgagtatagg aaaatcaatc tatcaagata cagaagtata ttttatagat
    13321 ttagagaata tagaggttat aagtgtctac aaaatggagg gtatgcagtt actttatgaa
    13381 gttttgtcag acacttataa acttaagaat ggttttagtt aactatcaga aacgaaggaa
    13441 agagtatgat ttttgacgat ttgaaaaaca tcacctttta caaagggatt catcctaatt
    13501 tagacaaggc tatcgactat ctctaccaac atcgtaagga ttctttcgaa ttaggaaagt
    13561 atgatattga tggagataaa gtctttctag ttgttcagga aaatgtcctc aatcaagctg
    13621 aaaatgatca atttgagtat cataagaact atgcagattt gcatttgctg gtagaaggac
    13681 atgaatattc gagctacggt tcacgtatca aagacgaggc agtagcattc gacgaagcga
    13741 gtgacattgg ctttgttcat tgtcatgaac actacccact cttgttgggt tatcacaatt
    13801 ttgcgatttt cttcccaggt gagccacatc agccaaatgg ttatgcaggc atggaagaaa
    13861 aggttcgaaa atatctcttt aaaattttga ttgattaaaa ataggatgaa ttgttttttt
    13921 gtaaagcttt gataatactc taccatgaaa ttgatctttg tgaggtagag aaatgagaat
    13981 aaaatattta aaaattggta tcttcttgtc agtctgtgta tgctacaaga gctagtttct
    14041 tggatggaca gggaattaca gttgatgaga tggcttggat aattaggggc attgtgaatg
    14101 cattgattgg tagatacata aaattaggta cttatgcggc taagtatggt attagtatgg
    14161 cacgctcgat cttaagtagg gcagctgcaa ctgcagcagc aagagtagga ttatcgacca
    14221 agatttctgg atggatttta cgagtagctg tgaatgtagc tgatgtatat ggtaattttg
    14281 ccaacaatat tgctgcagct tgggatgcac atgataaaat ccctaacaat ggtcgtataa
    14341 acttttaaaa tgcgagaatg aaagcacttt gtattttttt attgaatatg ttagcttgga
    14401 cagtgcttgc aatgataatt cgtggagggc tagatggatt tgatgagcat acttggagta
    14461 ctattttaat tgcttcgctg ttcggggtat atgattataa gcccatagat aaaaatagaa
    14521 aaagtccaaa agaaaaaaga aaaaatagat ttgttcatgg tagggattta tgaaagcttt
    14581 actgacaaaa aagaaaacag tttacaaaga aaaatgatgg aggagcaaac atggcacaaa
    14641 aaggagtaag ccttatcaag gcagcatttg atacagacaa ctttctcatg cgttttagtg
    14701 agaaagtctt ggacatcgtg acagccaatc ttctttttgt cgtctcttgt ttgcccatcg
    14761 tgacgattgg agtagctaaa atcagcctct acgagaccat gttcgaagtt aagaagagca
    14821 gacgggtgcc tgtttttaaa atctatctaa gatctttcaa gcaaaatctg aaactaggtc
    14881 ttcagctggg tttaatggag ttaggaattg tgtttcttac cctttcagat ctctatcttt
    14941 tctggggtca aacagctctg cccttccaat tgctgaaagc catttgttta ggtattctga
    15001 tttttcttac tatcgtgatg ctggctagtt accctatcgc ggcacgttat gacctatctt
    15061 ggaaagaagt gttacaaaaa gggcttatct tggcaagttt taactttcct tggttcttcc
    15121 tcatgttagc cattcttgtc ctcattgtga tggttcttta tctgtctgcc ttcagtctac
    15181 tcttaggtgg ctcagccttc ctactttttg ggtttggact tcttgctttt ttacaagtgg
    15241 gagtgatgga aaagctattt gctaagtatg agtaaggggt gcaattattt ctgaaactac
    15301 tttcaaaggc tccaaacgct attctataag cgagaaacta aaatctaagt gtctataaga
    15361 tattgaaagc cttttcggta aagtgtatac taaagaagta aagaaaaatc tgctttagca
    15421 gaaaataaaa ataataggag aaaatctatg tcagatttga aaaaatacga aggtgtcatt
    15481 ccagccttct acgcatgtta tgatgatcaa ggagaagtaa gcccagaacg tacgcgtgcc
    15541 ttggttcaat acttcattga taaaggtgtt caaggtcttt atgtcaatgg ttcttctggt
    15601 gaatgtatct accaaagcgt tgaagatcgc aagttgattt tggaagaagt catggcggta
    15661 gccaaaggta aattgaccat tattgcccat gttgcttgca ataatactaa agatagtatg
    15721 gaacttgctc gccatgctga aagcttggga gtagatgcta ttgcaacgat tccaccaatt
    15781 tatttccgct tgccagaata ctcagttgcc aaatactgga acgatatcag ttctgcagct
    15841 ccaaacacag actacgtgat ttacaacatt cctcaattgg caggggttgc tttgactcca
    15901 agcctttaca cagaaatatt gaaaaatcct cgtgttatcg gtgtgaagaa ctcttctatg
    15961 ccagttcaag atatccaaac ctttgtcagc cttggtggag aagaccatat cgtctttaat
    16021 ggtcctgatg agcagttcct aggtggccgc ctcatgggtg ctaaggctgg tatcggtggt
    16081 acttatggtg ctatgccaga actcttcttg aaactcaatc agttgattgc ggataaggac
    16141 ctagaaacag cgcgtgaatt gcagtatgct atcaacgcaa tcattggtaa actcacttct
    16201 gctcatggaa atatgtacgg tgtcatcaaa gaagtcttga aaatcaatga aggcttgaat
    16261 attggatctg ttcgttcacc attgacacca gtaattgaag aagatcgtcc agttgtagaa
    16321 gcggctgcag ccttgattcg tgaaaccaag gagcgcttcc tctaatctaa aaggaggtat
    16381 ttatgacata ctacgttgca attgatatcg gtggaactaa catcaagtat ggtttggttg
    16441 atcaagaggg gcaacttctt gaatcacatg aaatgccaac agaggcgcat aagggtggac
    16501 ctcatatctt acaaaagacc aaagatatcg tagctagtta tttagaaaaa ggcccagtag
    16561 caggtgttgc catatcttct gctgggatgg tggatccgga taagggtgag attttctatg
    16621 ctgggccgca aatccctaac tacgcaggca cccagttcaa aaaggaaatc gaagaaagct
    16681 ttactattcc ttgtgagatt gaaaatgatg tcaactgtgc aggtcttgct gaggcagtat
    16741 ctggttcagg caagggagca agtgtgacac tttgcttgac cattggaacc ggtatcggtg
    16801 gttgcttgat tatggatagg aaagtcttcc atggttttag taattcagcc tgtgaagtcg
    16861 ggtatatgca tatgcaggat ggagcttttc aggatctcgc ttctacaaca gctttagtga
    16921 aatatgtagc tgaagcccat ggagaagatg ttgatcagtg gaatggccga cgtatcttta
    16981 aggaagctac tgaaggaaac aaaatctgca tggaaggtat tgaccgtatg gttgactatc
    17041 taggaaaagg tctggcaaat atttgctacg ttgccaatcc agaagtggtc attcttggtg
    17101 gtggtatcat ggggcaagag gctatcctca aacctaagat ccgcacagcc ttgaaagagg
    17161 ctttggtacc aagtttagca gaaaaaacac gattagaatt tgcccatcac caaaatacag
    17221 cagggatgtt gggtgcatat tatcatttta agacaaaaca atcctagttt ggctcagcca
    17281 aactaggatt ttcttacacg tttttgtcta cgatagccgt tgagtttttt attttcccag
    17341 tagctattaa agattttttc cttgctttcg cgattgattt ccaaaaagta ggcataaatc
    17401 aaatcgataa agaagagcat aggaagttga gcggatattc gttggatata ggagggttgg
    17461 ctgtgggtgg ctacaagaac agtctctgta taggtctggc tatctttatt gggaacactt
    17521 gtaaagagta cagtctttgc ccccatctcc ttggcatcta atagactatc taaaatagaa
    17581 ggagttgagc ctgaaagtga gaagcccagt actagacaat tttcatccat gatgctggtt
    17641 gtccaggcaa agccgtcttg gtctgtcaaa gcttcgcaga ccacacctaa tcgcataaaa
    17701 cgtaatttca tttcacgggc gacgaggcca gaactccctg ttccaaagaa gtagatacgc
    17761 tcagcatctt cgattagctg ggcaattcgt tctagttgga tttcgtcaat caagtcttgt
    17821 gtttgttccc tcatattgct ataacttctg aggactcgtt tggtcagtgg actgtgcttg
    17881 gagacttggt tggcttgatt ttctgcctga tgttggtatt ggaaaataaa ttctcggtag
    17941 ccagtaaagc cacacttttt agcaaagcgg gtcaaagcag cttgagaaat atgtaatttt
    18001 tgggtgactt gttgagaaga taaatcatct gtaatcgttt cagcttgcaa aaaatagcga
    18061 gcgatttctt gctctaggtc tgtcatttct tcaaaatgtg aatcaatgat agttgcgata
    18121 tctggtttgt ccatagggaa agctccttta catgagtcat actggaagac tagatcaggg
    18181 aatagtcaca cttcattata acacataata taaggataga taaataaaaa cgcatctctg
    18241 ttttaaaaac gaaaaaatcg aaaaagcttc tctcttttcc ataattttct actcaaattg
    18301 tggtacaatt aagagtaaga ttttaagtta gaaatgagac tgatttgtat gagaaaattt
    18361 aacagccatt cgattccgat tcggcttaat ttattgtttt caatcgtcat tttacttttt
    18421 atgaccatta ttggtcgttt gttgtatatg caggttttga acaaggattt ttacgaaaaa
    18481 aagctagcct cagctagtca gaccaagatt acaagcagtt cagcccgtgg ggaaatttat
    18541 gatgctagtg gaaaaccttt ggtagaaaat acgttaaagc aggttgtttc ctttacgcgt
    18601 agcaataaaa tgacggctac agacttaaaa gaaacagcta aaaagttact gacttatgtg
    18661 agcatcagtt ctccaaattt gacagaacgc cagctggcgg attactattt ggctgatcct
    18721 gaaatctata aaaaaacagt ggaagctctc ccaagcgaga aacgcttgga ttcagatggc
    18781 aatcgtctat ccgaatcaga actgtataac aatgcggtcg atagtgtaca aacgagtcaa
    18841 ctaaactata cagaggatga aaagaaagaa atctatcttt ttagtcagtt aaatgctgtt
    18901 ggaaactttg cgacaggaac cattgcgaca gatcctctaa atgattctca ggtggctgtt
    18961 attgcctcta tttcaaagga gctgcctggc attagtattt ctacttcttg ggatagaaag
    19021 gttttggaaa cttccctttc ttctatagtt gggagtgtat ccagtgaaaa agctggtctc
    19081 ccagcggaag aagcagaagc ctatcttaaa aaaggctatt ctctaaatga tcgtgtagga
    19141 acctcctatt tggaaaagca atatgaagag accttacaag gaaaacgctc ggtaaaagaa
    19201 atccatctgg ataaatatgg caatatggaa agcgtggata caattgagga aggtagtaag
    19261 ggaaacaata tcaaactgac cattgatttg gccttccaag atagcgtgga tgctttactg
    19321 aaaagttatt tcaattccga gctagaaaat ggtggagcca agtattctga aggtgtctat
    19381 gcagtcgccc ttaacccaaa aacaggtgcg gttttgtcta tgtcagggat taaacatgac
    19441 ttgaaaacgg gagagttgac gcctgattcc ttgggaacgg taaccaatgt ctttgttcca
    19501 ggttcggttg tcaaggcggc gaccatcagc tcaggttggg aaaatggagt cttgtcagga
    19561 aaccagacct tgacagacca gtccattgtc ttccaaggtt cagctcccat caattcttgg
    19621 tatactcagg cttacggttc attccctatc acagcggtcc aagctctgga gtattcatca
    19681 aatacctata tggtccaaac agccttaggt cttatggggc aaacctatca acccaatatg
    19741 tttgtcggca ccagcaatct agagtctgct atggagaaat tgcgttcaac ctttggcgaa
    19801 tatggcttgg gtactgcgac aggaattgac ctaccagatg aatctactgg atttgttccc
    19861 aaagagtata gctttgctaa ttacattact aatgcctttg ggcagtttga taactatacg
    19921 ccgatgcagt tggctcagta tgtagcaact attgcaaata atggtgttcg tgtggctcct
    19981 cgtattgttg aaggcattta tggtaataat gataagggag gactgggtga cttgattcag
    20041 caactgcaac cgacagagat gaataaggtc aatatatccg actccgatat gagcatcttg
    20101 caccaaggtt tttatcaggt tgcccatggt actagtggat tgacaactgg acgtgccttt
    20161 tcaaatggtg ccttggtatc cattagcgga aaaacaggta cagccgaaag ctatgtggca
    20221 gatggtcagc aagcaaccaa taccaatgcg gtggcctatg ccccatctga taatccccaa
    20281 atcgctgtcg cagtggtctt tcctcataat accaatctaa caaatggtgt aggaccttcc
    20341 attgcgcgtg acattatcaa tctgtatcaa aaataccatc caatgaacta gaaaggaaat
    20401 tatgctttat ccaacaccta ttgccaagtt gattgacagt tattctaagt taccaggtat
    20461 cgggattaag acggctacgc gtctggcctt ttatacgatt gggatgtctg ctgatgatgt
    20521 caatgaattt gcaaaaaatc tcctttctgc taagagagaa ttgacatatt gttctatttg
    20581 tggacgtttg acagacgacg atccttgttc tatctgtact gatccgactc gtgaccagac
    20641 aacaatttta gttcttgagg atagtagaga tgtggcagcc atggaaaata tccaagaata
    20701 ccatggactc tatcatgtcc tgcatggcct catttctcct atgaatggta tcagtccgga
    20761 cgatatcaat ctcaagagcc ttatgactcg tcttatggat agtgaggttt cagaagtgat
    20821 tgtggcgact aatgctacag cggatggtga agcgacttcc atgtatcttt cacgtttgct
    20881 caagccggct ggtatcaagg ttacgcgtct agcacgaggt ctcgctgtgg gagcggacat
    20941 tgagtatgcg gacgaagtga cactcttacg agcgattgaa aatcggacag agttgtaagt
    21001 gtaggcaaat ttacgaactc ccttcattta taaaaaatca aagaggctga aaatcgttcc
    21061 tatcggcctc tttttgtata gtgtgatgag taggatcggg ttcaagtttt aaaaaaccaa
    21121 gcaaatatga tatactaaag agcgagtatt ctagtagaat taggacaaat aatatgaaac
    21181 aaacgattat tcttttatat ggtggacgga gtgcggaacg cgaagtctct gtcctttcag
    21241 ctgagagtgt catgcgtgcg gtcaattacg accgtttcac agtcaagact ttctttatca
    21301 gtcagtcagg tgactttatc aaaacacagg aatttagtca tgctccgggg caagaagacc
    21361 gtctcatgac caatgaaacc attgattggg ataagaaagt tgcaccaagt gctatctacg
    21421 aagaaggtgc agtggtcttt ccagtccttc acgggccaat gggagaagat ggctctgttc
    21481 aaggattctt ggaagttttg aaaatgcctt acgttggttg caacattttg tcatcaagtc
    21541 ttgccatgga taaaatcacg actaagcgtg ttctggaatc tgctggtatt gcccaagttc
    21601 cttatgtggc tatcgttgaa ggcgatgatg tgactgctaa aatcgctgaa gtggaagaaa
    21661 aattggctta tccagtcttc actaagccgt caaacatggg gtctagtgtc ggtatttcta
    21721 agtctgaaaa ccaagaagaa ctccgtcaag ccttaaaact tgccttccga tatgacagcc
    21781 gtgtcttggt tgagcaagga gtgaatgccc gtgaaattga ggttggcctc ttgggtaact
    21841 acgatgtcaa gagcacgcta cctggagaag ttgtcaagga cgttgccttt tatgactacg
    21901 atgccaagta tattgataac aagattacta tggatattcc tgccaaaatc agtgatgatg
    21961 tggtggctgt catgcgtcaa aatgcagaaa cagccttccg tgccattggt ggccttggtc
    22021 tatctcgttg cgatttcttc tatacagata agggagagat ttttctcaac gagctcaata
    22081 ccatgccagg ttttacccag tggtctatgt acccactact ttgggacaat atggggatca
    22141 gctacccagg actaatcgag cgtttggttg accttgccaa ggaaagcttt gacaagcgcg
    22201 aagcgcattt gatataaaat gaaagagagg gtagaagcca gaaccatcac tgcacggtga
    22261 ctagagttct cggacttcag ccctttttaa aggagtagaa atgaaattaa caatccatga
    22321 aattgcccaa gttgttggag ccaaaaatga tatcagtatc tttgaggaca cccagttaga
    22381 aaaagctgag tttgatagtc gtttgattgg aactggagat ttatttgtgc cacttaaagg
    22441 tgcgcgtgat ggccatgact ttattgaaac agcctttgaa aatggtgcag cagtaacctt
    22501 gtctgagaaa gaggtctcaa atcatcctta cattttagta gatgatgttt tgacagcctt
    22561 tcaatcccta gcatcctact atcttgaaaa aacgactgtt gatgtctttg ctgttacagg
    22621 ttcaaatggc aagacaacga ctaaggatat gttggcgcat ttactgtcaa caagatacaa
    22681 gacctacaaa acacaaggca attacaataa tgagattggc cttccttaca cagttctcca
    22741 catgcctgaa ggaacagaaa agttggtctt ggagatggga caggatcact tgggcgatat
    22801 tcatctcttg tctgaattgg ctcgtccaaa aacagccatc gtgaccttgg ttggagaagc
    22861 ccatttggcc tttttcaaag accgttcaga gattgctaag ggaaaaatgc aaattgcaga
    22921 cggaatggct tcaggttcct tgcttttagc gccggctgac cctatcgtag aggactattt
    22981 gccaactgat aaaaaggtgg ttcgttttgg gcaaggggca gagctggaaa ttactgactt
    23041 ggttgagcgc aaagatagtc tgaccttcaa ggccaatttc ttagagcaag cccttgattt
    23101 gccagtaact ggcaagtaca atgcgacaaa tgctatgatt gcatcctatg ttgccttgca
    23161 agaaggagtt tcagaggagc aaattcgttt ggccttccaa gatcttgaat tgacgcgtaa
    23221 ccgtaccgag tggaagaaag cagccaatgg agcagatatc ctgtcagatg tttacaatgc
    23281 caatccaact gctatgaaac tgattttaga gactttctct gccattccag ccaatgaagg
    23341 tggcaagaaa attgcagtgt tggcggatat gaaggagctt ggtgaccagt ctgttcaact
    23401 tcataatcag atgattttga gcctttctcc agatgtgctt gataccgtga ttttctatgg
    23461 acaagatatt gctcagttgg ctcaattggc cagtcaaatg ttcccaatcg gccacgttta
    23521 ctacttcaag aaaacagaag accaggatca atttgaagac ctagtcaagc aggtcaagga
    23581 aagccttgga gcccatgacc aaatcctgct caaaggctct aactctatga atctagccaa
    23641 gttggtagaa agtttagaaa atgaagacaa gtgattttgt caagtatttg caaagaatga
    23701 ttgccattac agatactggc ttaaccttta caaaagatcc gtttgaccgt gagcgctacg
    23761 aagacttgcg aagtctgtta tctgaaatgt tgaatcaagc atcagacctt gattccgaag
    23821 aagtggcaga agtcttgaag ccaacttctg cttatgcgac tcccttaatg gacgtccgtg
    23881 cttggattgt tgaggatgag aagatttgtc tggttagggg acaaggagag gatagttggg
    23941 ctttgccagg tggctttggt gaagtcggct attctccaac agaaaatatt ctcaaggaaa
    24001 ttgaagaaga aaccggtttt aaagccaaag ttgaaagact gctagctgtt tttgatacca
    24061 atcgtttcca actacagagc aaacagtata caaagtttgt ttttggatgt aagcttcttg
    24121 atggacaatt ccaagaaaat caagaaattg ctgaccttca attttttgcc attgaccaac
    24181 tgccgaactt atctgaaaaa cgcattacca aggagcaaat agagcttctt tggcaggttt
    24241 atcaaggtca tagggggcaa tatcttgact aagaagatga ttatcgtatt tctaaatcca
    24301 tttttaacaa ctagcatggt ataataatat gcaggaaaat tttgaattat gaggaagact
    24361 agatgaattt atgggatatt ttctttacga ctcaggcaac cgagccgccc aaatttgacc
    24421 ttttttggta tgttagccta tttacgctct tagccttaac cttttataca gcccatcgct
    24481 atcgtgaaaa gaaggtttac caacgatttt tccaaatctt gcagactgtt cagttaatcc
    24541 ttctttatgg ttggtactgg gtcaatcata tgccactgtc agaaagccta cccttttacc
    24601 attgccgtat ggctatgttt gtggtactct tgcttcctgg tcaatccaaa tataaacaat
    24661 actttgcatt attgggaaca tttgggacat tagcagcctt tgtttatcca gtgccagatg
    24721 cttacccttt tccacatatc accattctat cctttatctt tggtcattta gcactcttgg
    24781 ggaactctct agtttatcta ttgagacagt ataatgcgcg attgctggat gtgaagggaa
    24841 tttttctcat gacctttgcc ctaaatgcct tgatttttgt ggtcaatttg gtgacaggtg
    24901 gcgattacgg atttttgaca aaaccgccat tggttgggga tcacggtcta gtagctaatt
    24961 atttacttgt ttcaattgtg ctggtagcta ctatcagttt gactaagaaa atcttagaat
    25021 tctttttagc tcaagaagca gaaaaaatga ttgcaaagga agcttaacac agagctttct
    25081 tttttgctct tagagagttt ttacaagcag cttataaaat aagaatttct gaatagacaa
    25141 actcaaaaaa tggctgggaa atttaggaaa aaagcaagca cgattaaatt ttttgtgtta
    25201 taatattttg tgaatagcta tgcctatgtt tagctatgga ataatacgaa gtgcgaaact
    25261 tggaagatag agaggaagcg atgtaatggc tagagaaggc ttttttacag gtctagatat
    25321 tggaacaagc tctgtcaagg tgcttgtggc cgagcagaga aatggtgaat taaatgtaat
    25381 tggcgtgagt aatgccaaaa gtaaaggtgt aaaggatgga attattgttg atattgatgc
    25441 agcagcaact gctatcaagt cagccatttc ccaagcggaa gaaaaggcag gcatttcgat
    25501 taaatcagtg aatgtcggct tgcctggtaa tcttttgcag gtagaaccaa ctcaggggat
    25561 gattccagta acatctgata ctaaggaaat tacggatcaa gatgttgaaa atgttgtcaa
    25621 atcagctttg acaaagagta tgacacctga ccgtgaagtc attaccttta ttcctgaaga
    25681 atttattgtg gatggtttcc aagggattcg tgacccacgt ggcatgatgg gggttcgcct
    25741 tgaaatgcgt ggtttgcttt atacaggacc tcgtactatc ttgcacaatt tgcgtaagac
    25801 ggttgagcgt gcaggtgttc aggttgaaaa tgttatcatt tcaccactag caatggttca
    25861 gtctgttttg aacgaagggg aacgtgaatt tggtgctaca gtgattgata tgggggcagg
    25921 tcaaacgact gtcgctacaa tccgtaatca agaactccag ttcacacata ttctccaaga
    25981 aggtggagat tatgtaacta aagatatctc caaggttttg aaaacctctc gcaaattagc
    26041 ggaaggcttg aaactgaatt acggggaagc ctatccgcct cttgcaagca aagaaacctt
    26101 ccaagtagag gttattggag aagtagaagc agtcgaagtg acggaagcct acttgtcaga
    26161 aattatttct gcacgaatca agcacatcct tgaacaaatc aagcaagaat tagatagaag
    26221 gcgtctattg gacctccctg gtggtattgt cttaatcggt gggaatgcca ttttaccagg
    26281 tatggttgag cttgctcagg aagtctttgg cgtccgtgtc aagctttatg ttccaaatca
    26341 agttggtatc cgtaatccag cctttgcgca tgtgattagt ttatcagaat ttgcgggtca
    26401 attaacagaa gttaatcttt tggctcaggg agcgataaaa ggtgagaatg acttaagtca
    26461 tcagccaatt agttttggtg ggatgctgca aaaaacagct cagtttgtac aatcaacgcc
    26521 tgttcaacca gctcctgctc cagaagtaga gccggtggcg cctacagaac caatggcgga
    26581 tttccaacaa gcttcacaaa ataaaccgaa attagcagat cgtttccgtg gattgatcgg
    26641 aagcatgttt gacgaataaa gaggaaaaat aaattatgac attttcattt gatacagctg
    26701 ctgctcaagg ggcagtgatt aaagtaattg gtgtcggtgg aggtggtggc aatgccatca
    26761 accgtatggt cgacgaaggt gttacaggcg tagaatttat cgcagcaaac acagatgtac
    26821 aagcattgag tagtacaaaa gctgagactg ttattcagtt gggacctaaa ttgactcgtg
    26881 gtttgggtgc aggaggtcaa cctgaggttg gtcgtaaagc cgctgaagaa agcgaagaaa
    26941 cactgacgga agctattagt ggtgccgata tggtcttcat cactgctggt atgggaggag
    27001 gctctggaac tggagctgct cctgttattg ctcgtatcgc caaagattta ggtgcgctta
    27061 cagttggtgt tgtaacacgt ccctttggtt ttgaaggaag taagcgtgga caatttgctg
    27121 tagaaggaat caatcaactt cgtgagcatg tagacactct attgattatc tcaaacaaca
    27181 atttgcttga aattgttgat aagaaaacac cgcttttgga ggctcttagc gaagcggata
    27241 acgttcttcg tcaaggtgtt caagggatta ccgatttgat taccaatcca ggattgatta
    27301 accttgactt tgccgatgtg aaaacggtaa tggcaaacaa agggaatgct cttatgggta
    27361 ttggtatcgg tagtggagaa gaacgtgtgg tagaagcggc acgtaaggca atctattcac
    27421 cacttcttga aacaactatt gacggtgctg aggatgttat cgtcaacgtt actggtggtc
    27481 ttgacttaac cttgattgag gcagaagagg cttcacaaat tgtgaaccag gcagcaggtc
    27541 aaggagtgaa catctggctc ggtacttcaa ttgatgaaag tatgcgtgat gaaattcgtg
    27601 taacagttgt tgcaacgggt gttcgtcaag accgcgtaga aaaggttgtg gctccacaag
    27661 ctagatctgc tactaactac cgtgagacag tgaaaccagc tcattcacat ggctttgatc
    27721 gtcattttga tatggcagaa acagttgaat tgccaaaaca aaatccacgt cgtttggaac
    27781 caactcaggc atctgctttt ggtgattggg atcttcgccg tgaatcgatt gttcgtacaa
    27841 cagattcagt cgtttctcca gtcgagcgct ttgaagcccc aatttcacaa gatgaagatg
    27901 aattggatac acctccattt ttcaaaaatc gttaagtaaa tgaatgtaaa agaaaataca
    27961 gaacttgttt ttcgagaagt tgcagaggct agtctgagtg ctcatcgaga gagtggttcg
    28021 gtctctgtca ttgcagttac caagtatgta gatgtaccga cagcggaagc cttgcttccg
    28081 ctaggtgtcc atcatatcgg tgaaaatcgt gtagataagt ttctggaaaa atatgaagct
    28141 ttaaaagatc gagatgtgac ttggcatttg attggtacct tgcaaagacg taaggtgaaa
    28201 gatgtcattc aatacgttga ttatttccat gcattggact cagtaaagtt agcaggggaa
    28261 attcaaaaaa gaagtgaccg agtcatcaag tgtttccttc aagtaaatat ttctaaagaa
    28321 gaaagcaaac acggtttttc gagagaggaa ctgctggaaa tcttgccaga gttagccaga
    28381 ctagataaga ttgaatatgt tggtttaatg acgatggcac cttttgaggc tagcagtgag
    28441 cagttgaaag agattttcaa ggcggcccaa gatttacaaa gagaaattca agagaaacaa
    28501 attccaaata tgcctatgac cgagttaagt atgggaatga gtcgtgatta taaagaagcg
    28561 attcaattcg gttccacttt tgttcgtata ggtacatcat tttttaagta ggagagaacc
    28621 atgtctttaa aagatagatt cgatagattt atagattatt ttacggagga tgaggattca
    28681 agtctccctt atgaaaaaag agatgagcct gtgtttactt cagtaaattc ttcacaggaa
    28741 ccggctctcc caatgaatca accttcacag tcggctggca caaaagagaa caatatcacc
    28801 agacttcatg caagacaaca ggaattggca aatcagagtc agcgtgcaac ggataaggtc
    28861 attatagatg ttcgttatcc tagaaaatat gaggatgcaa cagaaattgt tgatttattg
    28921 gcaggaaacg aaagtatctt gattgatttt cagtatatga cagaggtgca ggctcgtcgt
    28981 tgtttggact atttggatgg agcttgtcat gttttagctg gaaatttgaa aaaggtagct
    29041 tctaccatgt atttgttgac accagtgaac gttattgtaa atgttgaaga tatccgttta
    29101 ccagatgaag atcaacaggg tgagttcggt tttgatatga agcgaaatag agtacgataa
    29161 tgattttttt aattcgtatg atttataatg cagtggatat ttactcccta attttggtag
    29221 ccttcgctgt catgtcttgg tttccaggtg cctacgaatc cagtttaggt cgttggattg
    29281 tagcgttggt aaaaccagtg cttgctccct tgcaacgcct gcctttacag atagcgggtc
    29341 ttgatttatc tgtttgggtt gcgattgttt tggttcgatt tttaggagaa aacctagtgc
    29401 gttttctggc gatgatagga tgaataaagg gatttatcag catttctcca tagaagatcg
    29461 tccatttctt gacaagggaa tggaatggat aaagaaggta gaagatagct atgctccttt
    29521 tttaactcct tttatcaatc ctcatcagga gaagctatta aagattttgg ccaaaaccta
    29581 tggtcttgct tgtagcagta gtggggaatt cgtctcgagt gagtatgttc gagttttatt
    29641 atccccagat tatttccaac cagagttttc agattttgaa atatctctcc aggaaattgt
    29701 gtattccaat aaatttgaat atttaacgca tgctaagatt ttagggacag tcatcaatca
    29761 attagggatt gaacggaaac tttttggaga tatcctagta gatgaagaac gggcgcagat
    29821 tatgattaat cagcagtttc ttctcctctt tcaagatgga ttaaagaaaa ttggtcgtat
    29881 acctgtttcg ctggaggaac gtcctttcac cgagaaaata gataagctag aacagtatcg
    29941 agaactggat ttatctgtgt ctagttttcg attagatgtt cttttatcaa atgttttgaa
    30001 actatctagg aatcaagcaa accagttgat tgaaaagaaa cttgtccaag taaattatca
    30061 tgtggtagac aaatcagatt acactgttca agttggagac ttgattagtg tgagaaaatt
    30121 tggtcgcttg agattacttc aagataaggg acaaacgaaa aaagagaaga aaaaaataac
    30181 cgtccagtta ttattaagta agtgaggaat agaatgccaa ttacatcatt agaaataaag
    30241 gacaagactt ttggaactcg attcagaggt tttgatccag aagaagtcga tgaattttta
    30301 gatattgtgg ttcgtgatta cgaagatctt gtgcgtgcga atcatgataa aaatttgcgt
    30361 attaagagtt tagaagagcg tttgtcttac tttgatgaaa taaaagattc attgagccag
    30421 tctgtattga ttgcccagga tacagctgag cgagtgaaac aggcggcgca tgaacgttca
    30481 aacaatatca ttcatcaagc agagcaagat gcgcaacgct tgttggaaga agctaaatat
    30541 aaggcaaacg agattcttcg tcaagcaact gataatgcta agaaagtcgc tgttgaaaca
    30601 gaagaattga agaacaagag ccgtgtcttc caccaacgtc tcaaatctac aattgagagt
    30661 cagttggcta ttgttgaatc ttcagattgg gaagatattc tccgtccaac agctacttat
    30721 cttcaaacca gtgatgaagc ctttaaagaa gtggttagcg aagtacttgg agaaccgatt
    30781 ccagctccaa ttgaagaaga accaattgat atgacacgtc agttctctca agcagaaatg
    30841 gaagaattac aagctcgtat tgaggtagcc gataaagaat tgtctgaatt tgaagctcag
    30901 attaaacagg aagtggaaac tccaactcct gtagtgagtc ctcaagttga agaagagcct
    30961 ctgctcatcc agttggccca atgtatgaag aaccagaagt agctccagtg catccgacag
    31021 gtccaacacc agctacagaa actgttgatt cagcaccggg atttgaagca ccgcaagaat
    31081 ctgttacaat tttataagaa atattctgag aacaatatct tatccttata ttcccagcga
    31141 gcaggagatg gtgtgagtcc tgcattccct atcgataaga ttatcctctc aaactatcaa
    31201 gtctgaatct agtaagattt gacgttcccc acgttacggg ataagaggga gaaagactaa
    31261 aactttttcc gaataaaggt ggtaccacga ttttcgtcct ttttggaagt cgtggttttt
    31321 aatttgttat tatttataaa ggagatacca tgaaactcaa agacaccctt aatcttggga
    31381 aaactgaatt cccaatgcgt gcaggccttc ctaccaaaga gccagtttgg caaaaggaat
    31441 gggaagatgc aaaactttat caacgtcgtc aagaattgaa ccaaggaaaa cctcatttca
    31501 ccttgcatga tggccctcca tacgctaacg gaaatatcca cgttggacat gctatgaaca
    31561 agatttcaaa agatattatt gttcgttcta agtctatgtc aggattttac gcaccattta
    31621 ttcctggttg ggatactcat ggtctgccaa tcgagcaagt attgtcaaaa caaggtgtca
    31681 aacgtaaaga aatggacttg gttgagtact tgaaactttg ccgtgagtac gctctttctc
    31741 aagtagataa acaacgtgaa gattttaaac gtttgggtgt ttctggtgac tgggaaaatc
    31801 catatgtgac cttgactcct gactatgaag cagctcaaat tcgtgtattt ggtgagatgg
    31861 ctaataaggg ttatatctac cgtggtgcca agccagttta ctggtcatgg tcatctgagt
    31921 cagcccttgc tgaagcagag attgaatacc atgacttggt ttcaacttcc ctttactatg
    31981 ccaacaaggt aaaagatggc aaaggagttc tagatacaga tacttatatc gttgtctgga
    32041 caacgactcc atttaccatc acagcttctc gtggtttgac ggttggtgca gatattgatt
    32101 acgttttggt tcaacctgtt ggtgaagctc gtaagtttgt cgttgctgct gaattattga
    32161 ctagcttgtc tgagaaattt ggctgggctg atgttcaagt tttggaaact taccgtggcc
    32221 aagaactcaa ccacatcgta acagaacacc catgggatac agctgtagaa gagttggtaa
    32281 ttcttggtga ccacgttacg actgactctg gtacaggtat tgtccataca gcccctggtt
    32341 ttggtgagga cgattacaat gttggtattg ctaataatct tgaagtcgca gtgactgttg
    32401 atgaacgtgg tatcatgatg aagaatgctg gtcctgaatt tgaaggtcaa ttctatgaaa
    32461 aggtagttcc aactgttatt gaaaaacttg gtaacctcct tcttgcccaa gaagaaatct
    32521 ctcactcata tccatttgac tggcgtacta agaaaccaat catctggcgt gcagttccac
    32581 aatggtttgc ctcagtttct aaattccgtc aagaaatctt ggacgaaatt gaaaaagtga
    32641 aattccactc agaatggggt aaagtccgtc tttacaatat gatccgtgac cgtggtgact
    32701 gggttatctc tcgtcaacgt gcttggggtg ttccacttcc tatcttctac gctgaagatg
    32761 gtacagctat catggtagct gaaactattg aacacgtagc tcaacttttt gaagaacatg
    32821 gttcaagcat ttggtgggaa cgtgatgcca aagacctctt gccagaagga tttactcatc
    32881 caggttcacc aaacggcgag ttcaaaaaag aaactgatat catggacgtt tggtttgact
    32941 caggttcatc atggaatgga gtggtggtaa accgtcctga attgacttac ccagccgacc
    33001 tttacctaga aggttctgac caataccgtg gttggtttaa ctcatcactt atcacatctg
    33061 ttgccaacca tggcgtagca ccttacaaac aaatcttgtc acaaggtttt gcccttgatg
    33121 gtaaaggtga gaagatgtct aaatctcttg gaaataccat tgctccaagc gatgttgaaa
    33181 aacaattcgg tgctgaaatc ttgcgtctct gggtaacaag tgttgactca agcaatgacg
    33241 tgcgtatctc tatggatatt ttgagccaag tttctgaaac ttaccgtaag attcgtaaca
    33301 ctcttcgttt cttgattgcc aatacatctg actttaaccc agctcaagat acagtcgctt
    33361 acgatgagct tcgttcagtt gataagtaca tgacgatttg ctttaaccag cttgtcaaga
    33421 ccattcgtga tgcctatgca gactttgaat tcttgacgat ctacaaggcc ttggtgaact
    33481 ttatcaacgt tgacttgtca gccttctacc ttgattttgc caaagatgtt gtttacattg
    33541 aaggtgccaa atcactggaa cgccgtcaaa tgcagactgt cttctatgac attcttgtca
    33601 aaatcaccaa actcttgaca ccaatccttc ctcatactgc ggaagaaatt tggtcatatc
    33661 ttgagtttga aacagaagac ttcgtccaat tgtcagaatt accagaggct caaacttttg
    33721 ctaatcaaga agaaatcttg gatacatggg cagccttcat ggacttccgt ggacaagctc
    33781 aaaaagcctt ggaagaagct cgtaatgcaa aagttatcgg taaatcactt gaagcacact
    33841 tgacagttta tccaaatgaa gtggtgaaaa ctctactcga agcagtaaac agcaatgtag
    33901 ctcaactttt gatcgtgtct gagttgacca tcgcagaagg accagctccg gaagctgccc
    33961 ttagcttcga agatgtagcc ttcacagttg aacgtgctgc tggtgaagta tgtgaccgtt
    34021 gccgtcgtat tgacccaaca acagcagaac gcagctacca ggcagttatc tgtgaccact
    34081 gtgcaagcat cgtagaagaa aactttgcgg aagcagtcgc agaaggattt gaagagaaat
    34141 aagattgaaa agtctaggca aaattcaatt tgagaagaaa agacaactaa ttttatagtc
    34201 tattaaacgc attgtatcac gtttttgaac acctgatatg atgcgttttt tatttatttt
    34261 aaaaatttgc gaggtatgac tttttatact caacaagaat caaagagaaa cttagcaagc
    34321 taacagtagt aagataaaat agga
//
LOCUS       DAVLWA010000014        33651 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLWA010000014 DAVLWA010000000
VERSION     DAVLWA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.014"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(224..442)
                     /locus_tag="VYN80_000648"
     CDS             complement(224..442)
                     /locus_tag="VYN80_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1540600.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            600..746
                     /locus_tag="VYN80_000649"
     CDS             600..746
                     /locus_tag="VYN80_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540601.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            complement(1338..2027)
                     /gene="rplA"
                     /locus_tag="VYN80_000650"
     CDS             complement(1338..2027)
                     /gene="rplA"
                     /locus_tag="VYN80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1540602.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2235..2660)
                     /gene="rplK"
                     /locus_tag="VYN80_000651"
     CDS             complement(2235..2660)
                     /gene="rplK"
                     /locus_tag="VYN80_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1540603.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(2783..3499)
                     /locus_tag="VYN80_000652"
     CDS             complement(2783..3499)
                     /locus_tag="VYN80_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1540604.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3517..3978)
                     /locus_tag="VYN80_000653"
     CDS             complement(3517..3978)
                     /locus_tag="VYN80_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1540605.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            complement(3980..5017)
                     /locus_tag="VYN80_000654"
     CDS             complement(3980..5017)
                     /locus_tag="VYN80_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1540606.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSVAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            complement(5115..6440)
                     /gene="brnQ"
                     /locus_tag="VYN80_000655"
     CDS             complement(5115..6440)
                     /gene="brnQ"
                     /locus_tag="VYN80_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1540607.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6611..6703)
                     /locus_tag="VYN80_000656"
     CDS             complement(6611..6703)
                     /locus_tag="VYN80_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1540608.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6746..6898)
                     /locus_tag="VYN80_000657"
     CDS             complement(6746..6898)
                     /locus_tag="VYN80_000657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1540609.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(7058..7636)
                     /locus_tag="VYN80_000658"
     CDS             complement(7058..7636)
                     /locus_tag="VYN80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1540610.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7690..9090)
                     /gene="pepV"
                     /locus_tag="VYN80_000659"
     CDS             complement(7690..9090)
                     /gene="pepV"
                     /locus_tag="VYN80_000659"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1540611.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9103..9708)
                     /locus_tag="VYN80_000660"
     CDS             complement(9103..9708)
                     /locus_tag="VYN80_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1540612.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            9879..10679
                     /locus_tag="VYN80_000661"
     CDS             9879..10679
                     /locus_tag="VYN80_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540613.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10863..11696)
                     /locus_tag="VYN80_000662"
                     /pseudo
     CDS             complement(10863..11696)
                     /locus_tag="VYN80_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11686..13533)
                     /gene="uvrC"
                     /locus_tag="VYN80_000663"
     CDS             complement(11686..13533)
                     /gene="uvrC"
                     /locus_tag="VYN80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1540614.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(13573..14274)
                     /locus_tag="VYN80_000664"
     CDS             complement(13573..14274)
                     /locus_tag="VYN80_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1540615.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14353..15585)
                     /gene="murN"
                     /locus_tag="VYN80_000665"
     CDS             complement(14353..15585)
                     /gene="murN"
                     /locus_tag="VYN80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1540616.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15590..16810)
                     /gene="murM"
                     /locus_tag="VYN80_000666"
     CDS             complement(15590..16810)
                     /gene="murM"
                     /locus_tag="VYN80_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1540617.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16922..17701)
                     /locus_tag="VYN80_000667"
     CDS             complement(16922..17701)
                     /locus_tag="VYN80_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1540618.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17770..19431)
                     /locus_tag="VYN80_000668"
     CDS             complement(17770..19431)
                     /locus_tag="VYN80_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1540619.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19606..21828)
                     /gene="recJ"
                     /locus_tag="VYN80_000669"
     CDS             complement(19606..21828)
                     /gene="recJ"
                     /locus_tag="VYN80_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1540620.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22105..22864
                     /locus_tag="VYN80_000670"
                     /pseudo
     CDS             22105..22864
                     /locus_tag="VYN80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            22904..23668
                     /locus_tag="VYN80_000671"
     CDS             22904..23668
                     /locus_tag="VYN80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1540621.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23681..24358
                     /locus_tag="VYN80_000672"
     CDS             23681..24358
                     /locus_tag="VYN80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1540622.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFCIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24368..25027
                     /locus_tag="VYN80_000673"
     CDS             24368..25027
                     /locus_tag="VYN80_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1540623.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKIKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVPVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25323..26513
                     /locus_tag="VYN80_000674"
     CDS             25323..26513
                     /locus_tag="VYN80_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1540624.1"
                     /translation="MKSIKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCSPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26834..27715)
                     /locus_tag="VYN80_000675"
     CDS             complement(26834..27715)
                     /locus_tag="VYN80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1540625.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27856..29184)
                     /gene="vncS"
                     /locus_tag="VYN80_000676"
     CDS             complement(27856..29184)
                     /gene="vncS"
                     /locus_tag="VYN80_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1540626.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(29181..29837)
                     /gene="vncR"
                     /locus_tag="VYN80_000677"
     CDS             complement(29181..29837)
                     /gene="vncR"
                     /locus_tag="VYN80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1540627.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29886..29969)
                     /gene="pep27"
                     /locus_tag="VYN80_000678"
     CDS             complement(29886..29969)
                     /gene="pep27"
                     /locus_tag="VYN80_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1540628.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(30149..31528)
                     /gene="vex3"
                     /locus_tag="VYN80_000679"
     CDS             complement(30149..31528)
                     /gene="vex3"
                     /locus_tag="VYN80_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1540629.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31580..32227)
                     /gene="vex2"
                     /locus_tag="VYN80_000680"
     CDS             complement(31580..32227)
                     /gene="vex2"
                     /locus_tag="VYN80_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1540630.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32240..33517)
                     /locus_tag="VYN80_000681"
     CDS             complement(32240..33517)
                     /locus_tag="VYN80_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540631.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9562 a         7686 c         6228 g        10175 t
ORIGIN      
        1 ataaactaac ctcctcatta ttagcaaggc ttctactgat tataacctaa ttcttagact
       61 acttcaagac taactgtcca ataataatct acagataaat catccactct taatcaaatt
      121 ttattcaatc catctactca ttcccaaaaa gttgctgtat aatgcaagac atttttctta
      181 ctgcttttac ttttccttac cctcaattta gtgagctaat aaattattct tcttcaaaag
      241 gttgagattt atctagtaac tctaaaacat taatggtagg tagcaacatt gcttcactgc
      301 tatcaattgc cgaaactatt gaaacaatag aacgtaaata aggataaaga atagcagtac
      361 catttacgta tagaatatcg gtgatatttt catctatatt tatttcaaaa tatcctatta
      421 cagttacaga tatttttttc atggcatctt tgtcaactaa agaaacacta agttttacgg
      481 ctccatggct ataatcatct gtatgaccaa ccttaactat gggatataat atatcgaagg
      541 taaatgtgaa caaactgatt aggaaattta aaccaacttc taacaatgtg ttatactcaa
      601 tgaaaaccaa agagcaaact aggaaactag ccgcaggttg ctcaaaacac tgttttgagg
      661 ttgtggatag aactgacgaa gtcagtaacc atacgcacgg caaggcgacg ttgacgtggt
      721 ttgaagagat tttcaaagag tattagaaag ctcgatggac tattccagat tcattccact
      781 agatttcgaa atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtgccgt
      841 tcggcatacg gcggttcaac taacttttaa cgcatgtcgt tcaaggtaat aatccaaaca
      901 cgaaaccagt ccacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt
      961 ctgagctact atagtagatt gaaactagaa tagtacacct ctacttctaa aacattgtta
     1021 gaaatcgatt tgactgtcct gaacgattcg tcctattctt atttcatttt attataattg
     1081 atagtggtcg ccccagccag ataccttatc cactatatag gccttattat cacgttcctg
     1141 ttcgtagagc cacgatttcg ctatcccttc ctctgacgat tcttatttcc agcgtcagac
     1201 ttgggaatcg ctattgggtt caccggtagt aggtgcccac cgaggactta cacctcagat
     1261 atacgacatg cccgtcgtac atcaaaaaaa gttgagacat agtgtcttca acttttttac
     1321 attactttaa ctactgatta aagtgagttt acgtcaactt tgataccaac accttgagta
     1381 gttgtgatag tcaagtttgt tacgtaagtt cctttagctg tagctggttt tactttttgg
     1441 attgtttcgt tgaaagcttt aaagttttca accaattttt cagcttcaaa tgatacttta
     1501 ccgatgattg cttgaacgtt acctgcacgg tcagcacggt aagtgatttt accaccttta
     1561 gactcttcaa ctgctttagc aacatccatt gttacagtac cagttttagg gtttggcatc
     1621 aagttacgtg gtccaaggac acgtccaaga cgtccaacaa gagccatcat atcaggtgta
     1681 gcgataacta cgtcgaagtc caaccaaccg tcgttgattt tagcaacaag gtcatcttca
     1741 ccaacaaagt ctgcaccagc agcttttgct tcttcagctt ttgcaccacg tgcgaaaaca
     1801 agaacacgtg aagttttacc agtaccgttt ggcaatacca ttgctccacg gatttgttgg
     1861 tcagcttttt taacgtcgat gttcaagttg taagcaactt ctacagttgc atcaaatttt
     1921 gcaaagttag tttcttttgc aagtgctaca gcttcttcta cactgtatgc ttttgtgctg
     1981 tcgattttct caagagcagc acgaagttgt ttgctttttt tagccatttt ctattctcct
     2041 tgtaagtggt tcaatcgatt ttcatctccc acgtcacttc tcgaaatgat gaagtcttgc
     2101 gggttttcag tctactattt ttctcttcct acggccttaa gctcccttgc tgtatccaag
     2161 tacaagcctc agtcacttgc ctagtatgaa aagcaactaa tagactgctg tgagattttc
     2221 tactgtgtta ttgattagtc aacaacagtg aatcccatag aacgagcagt accttcgatc
     2281 atacgcattg cagactctac gtttgctgcg ttcaaatctg gcatcttagt ttctgcaatt
     2341 tcttgtactt gtgcacgagt aactgtagca actttagttt tattaggtgt acctgatcct
     2401 ttttcaacac ctgcagcttt tttcaaaaga acagcagctg gtggtgtttt cgtaacgaaa
     2461 gtaaatgatt tatcttcgta aactgagata acaactggaa tgatcatacc agcttggtca
     2521 gctgtacgag cgttgaactc ttttgtgaat cccatgatgt tgataccagc ttgaccaaga
     2581 gcaggtccaa ccggtggagc tggtgtagct ttaccagcag ggatttgcaa ttttacaagt
     2641 ttttcgactt ttttagccat ttttaaatcc tcctttgtgg ttttggcggt aattagagat
     2701 ttttacctcc cacaagtatg ctttacacat acctttccat tatacactat ttatctaaaa
     2761 atgcaagaga aaactttcat ttttaatcga cataatcgcc gccttcaaag ccatagtact
     2821 cttccaaact gagaccactt gcagcaattt ctttggcttc ttttccaacg tagcggagat
     2881 gccattcttc tgccatgtag ccagtctcct tttccttgcc tttaagatag cgaacaacaa
     2941 agccatagtc agccgcatgg tccaagagcc actgggctgc cttttcttca gtaactaaat
     3001 ccccatctgt cccaatcaaa tcaaaagcaa gacccgtctg gtgttcactg taacctgggc
     3061 gtgctgagta gcgatctgct gcttccttac catcttgatt gacatagtct tggtagagct
     3121 tagcctgagt ttcataactc ctaaacccac tgtattggtc actgattgga tagccctcag
     3181 cctgcatagc tgcgattagt ttgagcaact ctgccttggc tgttggattt tctcctggat
     3241 tgtaatcttt tgacaaagga taacgtttat tagctaggat aatctcatcg tatttccctt
     3301 ggatactata atagctccct ttattgctaa cttctgcctt tttctgacta gaagcttgag
     3361 atttgctacc tacagttcct tccggttgac tgctttgctc cgttttttga gtgttttctt
     3421 caactttggt tttttcttgc gaacaagctg caagggtcaa tgctagtaaa ccagataaaa
     3481 tgacatatct ttttttcatt tctttcttcc tctctcttag gccaaatact tctccaattc
     3541 atgagataag attttaatca tctcttccag ctctggcgat tgtacttggc aatcttctgc
     3601 cgccatttct tcccagggca cccaccagac tgggccacgg ccattcaaac cgtgactctt
     3661 catatcacct gctcttcggg gaaaaagatg ccaatgagcg tgggcatcgc catttcctag
     3721 gagttcaata ttcatctttt cagcctcaaa tgctttggcg actgcttctt ggactaaact
     3781 catttcctct aaaaagcgaa gttttacaga agtttccata tgatggagtt ctgtgacatg
     3841 atcttttgct aaaaataagg tatagccctt aaagtattgg tagtcaccaa taacaacata
     3901 gcctgtttct agttctttta caaagtaggg attttgccca gctttaatga gttcgattct
     3961 ctgacagatt aagcacattc tatgataccg cctcgctctt aagataagca tcaaccatac
     4021 gctcagtcgc tattactgaa tcgatgtggg tgcgctcata agagtgactg gactcaatac
     4081 cagctccaag aagtgcatgc ttgacctctg cacctgctga catcgctgct gaagcgtccg
     4141 atccgtaaaa tggatagatg tcgagcttaa atggaatatc ttgctcttta gccaaggcaa
     4201 ccaagtgttg acggaagtca tagtgataag gacctgaagc atccttgaca cagatagata
     4261 ctgtgtactc atctgtctgc tggtcatctc ccatagctcc catatccaca gccagatatt
     4321 ctactacctg agcaggaatg ttagaattag caccatgtcc tacttcttca aaaactgaaa
     4381 aagcaaaatg agttgttact ggcaattcaa tcttctcttt cttataaata cgaagcaggt
     4441 tgagcaaaat cgctacgctg accttatcat ccaaatgacg agacttgata aaacctgtat
     4501 cagtcacaac agttcgtgta tcgaagctga taaaatcacc gacctcaatc ccaagagcac
     4561 gagtttcttt ttcattgctt acttttacat ccaaacgcac ttccatattg tcctgcgtgc
     4621 gttctgcagt tcctgcatcc ttgtagacat ggcaagaagt ttggtggatg aggatggttc
     4681 ctgatacctt ttgacctgtg ctagccacat gaacggtaca gttttcacct tcaatcatgt
     4741 tccaaggaaa accaccgata cggtccaatt taagacgacc atctggtttg acagcacgaa
     4801 caatagcacc cagcgtatcc acatgggcag tcacatagcg atgttgctca tcattttgac
     4861 ctttgatggt cacattgaca ccacccttag ctgtgcgaac tggctggtaa ccaaaacctt
     4921 ctagagtctt gactaaataa ttcgaaattt cacgagtgaa gcccgttggc gatgcgatgg
     4981 ctgtcagttc tttgatatat tctactgttt gattcatttc ttcacctctt tcgctaccta
     5041 ttataacaca tttatcatcg gataaaaaaa gaaaatcact cctgtagact tggctacaaa
     5101 agtgatttaa gtgattattc catttcaaaa acatcgcttt cttgcttgtt tggtagaacc
     5161 aatgagagca agattccaac aatggctggc accaaccatg ggagagatgc cttggcaaaa
     5221 ggaagaacgc taacaagatt tgcaagaaac tcaaccttaa acgagcttcc tagtacgctt
     5281 gcaatggcaa taactgtaac cacagcaatt gtcaactgca tacctggttt tgaaagggca
     5341 acaaatttgt tgacaatgac aatcataacg atagcaatcg tgattgggta caagataacc
     5401 agtactggaa ttgagtactt gataatcgca tcaagaccca aattggcaat agcaaatcca
     5461 atcaaggtaa aggctgtcgc ataaaccttg tagctgattt gtgggaagcg ctcattaaag
     5521 aactcagctg ttgacacaat caaaccaaca gtcgttgtga agcaggttac ggtaaccata
     5581 gctgcaagga agagttgagc tgttgagcca aagatttctt gagtggcttg tgacaagatg
     5641 taaacacctg gtgttccacc cttcatcgct tcagctggta ctgggaaatg atttccaaga
     5701 aaacctaaac cgatgtaaag agcgctgaag gcaagggcaa caacgatacc aacaacccaa
     5761 atagttgaaa tgtattcttt cttacttgaa aatccaagtt gtttcaaggt ttgaactgcg
     5821 attacgctaa aggccactga agcaagggcg tccaaggtat tgtaaccttc taggaaacct
     5881 gtaccaaagg cagaagcttg ataagcagct gaagcagctt gaggacttgt tccaccatat
     5941 ttgatagctc ccagaacgac caagataaca atcaaaattg caaagactgg cgttaaaaca
     6001 cgtccaatgc ggtctaagat ttttgatgga ttaagcgaaa tcaaataggc tgccgcaaaa
     6061 tacagaaccg taaatacaat caagccaagt cctttatttg catccgacaa aagggggcta
     6121 atccctactt cgtaagctgt tgtagcagta cgtggggtag caaagaatgg accgattgac
     6181 aagtaaagaa ctgagaggta aagagtcgca aaccaaggcg ctatcttcgt tgaaatctcg
     6241 tagatatatc ctttaggatt tagcgttcca ataataaggg tcaagacggc gataccaacg
     6301 cctgaaaaga caaaacctgc gatggcagga agaaaatgtt ctccagatag agcacctaga
     6361 gaaggcggaa aaatcaagtt ccccgcacca aaaaatattc caaacaggag caaacctgtt
     6421 agggtacctt ttttagccat aaaaatctcc ttcatattta taaaatactg acctagtata
     6481 ccacgattag gagtctgaaa aaagcatcac gaggcaaata ttaaatgttt tcaattttct
     6541 taaaaataag aattatctaa aaataaaact ctcctaccca gtccacacca agtaggagaa
     6601 atgcaaaatt ttaaagttct tcaaaggcta acatgccacc ctggacattg ataacattat
     6661 aaccttgttc taacaggaat tggcaagcac gcgccgatct cattccagat ttgcaaataa
     6721 tataatgcaa gcgatctttg tccaattaat cataactatc agccaattga ctaagcggta
     6781 ggttgtgggc acattctaaa tgaagagctg caaactcatc cacttctctc acatccacta
     6841 gagaaagctg gtcgttttgg taaagctggt aaaatgcgtc aaaggctatt tctttcattc
     6901 tttttcttct aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtgc
     6961 gtatgttacc gacttcgtca gttttatctg caacctcaaa gctgtacttt gagcagccta
     7021 cgactagctt cctagtttgc tctttgattt tcattgacta taaaatggtt ttaattcttt
     7081 ttttcaaatc tggcactact tctgcctcaa accaaggatt tttggccatc cagatttgat
     7141 ttcgtggtga tgggtgaact agcggaaaat aggctggcag atagtcttta tagtgtttca
     7201 ccctctccgt taccttccca ctgattttct cctgtaaata gtaggcttgg gcatattgcc
     7261 caatcaagag ggttaactga atatcaggca attcctgtaa gagctgcgga tgccattttt
     7321 ctgcaaaacc tgtacgaggc ggaagatcac ccgacttgcc atgtcctgga aagtagaaat
     7381 ccataggcaa aacagcaaaa taacctgaat tgtaaaaggt atcttcatcc acacctagcc
     7441 agtcccgcaa gcggtcacca cttttatctt tccagtaaag gcctgcttct tgagttttaa
     7501 gtcccggagc ctgaccgatg atattgatgc gagcagtttt tggcgctgca aagagaggct
     7561 caatgccacg ctctgtatag ctggcattct gtgaatccgc catgatagcc tgtttgattc
     7621 tttcgatttg agacatctac tttccctcca aaaagaagtc caagcataag atctcagact
     7681 tctatcgttt tatttgatca attcgtaaat agcttcggca tagattgctg ctgctcggaa
     7741 gagatcgtcc aaggcgataa attcattggc ttggtgcatg gtatcaattg agtctgggaa
     7801 catagcacca taggcaactc cgcgctctaa caagcgacca aaggttccac caccgatgac
     7861 ttgttcatga cctttaaagc cagtttgttt ttcatagata ttcaacaagg tttgcacaag
     7921 tggatcttcc attggcacat agtgaggcgt gtgaccgtgt tcagacaggc taacagaaac
     7981 aactggcaag ttttcaagga ttgacttgat ttgttctgga cttgttcctt ttggatagcg
     8041 gatgttgagg gcaatggtat tatcagcact tgtttcatcg aagcggaaga cgccggcatt
     8101 catagaaaga gcacccatct tttcatccac atgagcaatc ttaagatttt caccctcatg
     8161 atcgttcaag agaattttac ctgcgatgtc aaggtagtct ttggctggac cagcaaagcc
     8221 aaactgactg aggaagaggg caaggtaagt tgcgccattg acacctgaag caggcatagc
     8281 accgtgggct gatttaccaa tgatcgtcac cttgtatttg ccagcttctt cttggagttc
     8341 tcctctaagt ttgtgttctg caacaaaggc atctagttta gcttgcaagt cagccaagtc
     8401 acctgaaacg actgctgttg ctgattctgg taccatattt tcacgtaaac cacctgtaaa
     8461 gctgtgaaga cgggcaacac ctgtattttc tcctgcaaag tggaggtatt ccgtgatatt
     8521 tcctttttca ccattgatga ttggaaattc agcatctggt gagaaaccga aatctggttt
     8581 ggcaagtcct acgtgctcaa agtagtagtc catgtctgcc cagcctgatt cttcgtctgt
     8641 tccaacgatg aagcgaactt tcttagaagt tggaagaccc aattctttga tgattttcaa
     8701 accatagtaa caagctgttg taggaccctt atcgtccgaa gccccgcgcg cataaaggcg
     8761 accatctttg atagttggtg tgtaagggtc tgtgtcccaa ccgctaccag caggcaccac
     8821 atccatatgg gcaaagattc cgagaacttc ttctccatca ccaaactcaa aatgtcctgc
     8881 atagttatca acattcttag ttgggtagcc atcgcggtct gcgatttcaa ggaatttctc
     8941 caaggctttt actggaccag gcccaaatgg atgctgggca tcagccttgc tgtcatcacg
     9001 ttctgaattg atttccaaaa ggctaaacaa gtcagccaag aggtcttctt tgcgtttttc
     9061 tacttctgct gtaaaatcaa ttgctgtcat tttttcttct ttctatcttt tctcgatgat
     9121 ttcatctact ggcaagcggt agcttggttc caatttttcg tctgtataac ccactgtgat
     9181 caagagttct gggcggaaac ggtcttcgat ttccaaaact tcattaactt ttgatttgtc
     9241 aaaaccaaga ataatgttag aaccaattcc ttggtctgtc aatgcaagaa ccaagttcat
     9301 ggcaaccaaa cctgcattga gagctaggta gtcgctgact tgttgttcat tgtagcgtgc
     9361 aaattcagct ggcaaatttt tcatgaagta ttgaagttgt tcttctgaga aattgttagc
     9421 accaccaact cgggcaatct tacgagcgcg tttggcaagg tctgtatctg taaatagggc
     9481 aatggttaca ggtgctgatg atacctgctc aaagtttgaa ccataagcca actttgccag
     9541 ttcagcattt ttctcacgaa caactacaaa tttccaaggc tggctgttgt gggcgcttgg
     9601 cgccaaggtt gcaatttcga tagccgtacg cacatctttt ggatcaacag gcttatcagt
     9661 gaaatgcttg gtcgcatgac gttttttatt taattcaaga aatttcataa tcgatttcct
     9721 tttctaattc tactgcttcc attctaccat aatttgaaag agcttgcagg gatttttcat
     9781 gattaagttt ttctatcaca gacataaaag atatatattg gtttatcagg aaagtttctg
     9841 ataaactagc aagtacttta catttgaatg gagacagtat gaaaaaattt agcctattac
     9901 tagctatcct accatttttg gttgcctgtg ggaatcaagc tacacccaaa gagactagcg
     9961 ctcaaaagac aatcgtcctt gctacagctg gcgacgtgcc accatttgac tacgaagata
    10021 aaggcaatct gacaggcttc gatatcgaag ttctaaaggc agtagatgaa aaactcagcg
    10081 actacgagat tcaatttcaa agaaccgcct gggaaagtat cttcccagga cttgattctg
    10141 gtcactatca ggctgcggcc aataatttga gttacacaaa agagcgtgct gaaaaatacc
    10201 tttactcgct tccgatttcc aacaatcccc tcgtccttgt cagcaacaag aaaaatcctt
    10261 tgacttctct tgaccagatc gctggtaaaa caacacaaga ggataccgga acttctaacg
    10321 ctcaattcat caataactgg aatcagaaac acactgataa tcccgctaca attaattttt
    10381 ctggtgagga tattggtaaa cgaatcctag accttgctaa cggagagttt gatttcctag
    10441 tttttgacaa ggtatccgtt caaaagatta tcaaggaccg tggtttagac ctctcagtcg
    10501 ttgatttacc ttctgcagat agccccagca attatatcat tttctcaagc gaccaaaaag
    10561 agtttaaaga gcaatttgat aaagcgctca aagaactcta tcaagacgga acccttgaaa
    10621 aactcagcaa tacctatcta ggtggttctt acctcccaga tcaatctcag ttacaataac
    10681 actcttcgaa aatctcttca aaccacgtca gctttatctg caacctcaaa gctgtgcttt
    10741 gagcaaccta cggctagctt cctagtttgc tctttgattt tcatattgag tataagatat
    10801 attaaaagcg atgggatcag ccaatcgctt ttttagtttg cattggttat tttaggaaac
    10861 tcttacaaaa aatcaaaaaa attcttcaca tcctctacat acctatgctt ttctattaag
    10921 cttgctaata gttccagatt gtgcctctga tagttatctt cacgacgtag ttcttcatac
    10981 atttcgataa agggaaaacc cttggatttg gcaaattcta actcagcctc gtaatcataa
    11041 gcgactttac gaaataggat atttaccaat tccccatctt caacctctat cacggcatac
    11101 tgggcacggt gattttttaa cgcctcccaa ttaaaatagg gcatgccaat cgaccctgga
    11161 ttgatgattt gttgcccttg actaccataa cgaagcaact acttgtgaac atgaccataa
    11221 actgccacgt ccgtttccgc atctagcagt tggtcaaatt tctctgtatc attctcaact
    11281 agcaagtcac caccatagtt tttgtcaggt aaattatgag agatagaaaa gcgcaatccg
    11341 tcaatttctt tcttttccag caaaggcaag cttcgtagcc agacaatcgt tgcaggatcc
    11401 attcgctcca tcaaatactg tgtcatacgc aagagctgaa cttcctgtgg gtcttctaag
    11461 ccatattgcc catctaaagc ctcaaggaca caatcatccc aattgcctcg aacacttgcc
    11521 gtgataggaa gttcctttaa cagagcgacc aagtcatttg cacctggacc agggaggaaa
    11581 atgtctccca tgagccaata ttcactggct ccttgagttt tagcatctgc aatcactgct
    11641 tctagcgccg tcgcattgcc atgaacatct gataaaattg cgattttatg gtttattgtg
    11701 gtttccttcc gtttggtaat ctactctttc ttccgccact tgaagcaaga tttctgtttc
    11761 ctgcgggttc aacttggttt gcacagcctc tgctactgct cttggtactc caacttcgac
    11821 aatctcatcc acactggctt ccttgatttt ggtcagagac ttgaagtgcc tcatgagatt
    11881 ttgcttgcgt ttaggtccca gaccatcaat cccatccaat tgagatgaga aagaattttt
    11941 ggagcgcagt tggcggtgga aggtaatggc aaagcggtgg acctcatctt gaatgcgttg
    12001 gaggaggaaa aattcctgag aattgcgaga caagtccacc acttccagcg gatctccaaa
    12061 gagcaattca tgggtttggt gcttatcatt cttttgcaga cctgcaattg gaatatccaa
    12121 gcccagttct tcttggatga cttgcttagc aatattgact tgaccttgcc ccccatcaat
    12181 cacaatcaaa tctggaggag tcaaagcctc acgctgtact cgaccatagc gtctgcgaat
    12241 gacctctcgc atactggcat agtcgtctgg cccgactacg gtcttgattt tatacttacg
    12301 gtaatccttc ttactaggct tgccgttgac aaagaccacc atggctgaaa caggactagt
    12361 tcccatgatg ttggagttat cgaaggactc gatgcgaact ggggttggga tttgaagcaa
    12421 gcgtcctaga ttttcaatag ctccttgggt cttctcgaca gatttctcta gcaggttgaa
    12481 cttctgctct aggctaacac gggcattttt tatagctaaa ttgaccagct gctttttctc
    12541 tccacgttga ggcttaagaa tcttggaatc caccaaggcc ttgacagcct cttcgtcaat
    12601 atcttgcgga atcagtacct cattgggaac tagatgggat ttttcttgat agaattgtcc
    12661 tacataggtc aaaaaatcct catctggatc attgaagtag gggaagagat tgacatcgcg
    12721 ctcgatgagc ttaccctgac ggacaaagaa aacctgcaca cacatccagc ccttatccac
    12781 atagtagcca aagacatcgc gattttggag atccttagcc atgacccgtt gcttggttcg
    12841 aagcgttcca atagcctgaa tcaggtcacg gtattccgcc gcacgttcaa actccatagt
    12901 ctgggctgcc gctgccattt tgccctttag ctcatcaatg attttgttat cctgcccttt
    12961 cagaaaatca gaaacctcct gggccataga cttgaaataa atctcatcct tcttacagat
    13021 ggtgtgggcc atgcattgac cgatatggta ataaaaacag accttagagg gcgggttggt
    13081 acacttacga aaagggaata tccgatccag caaccgcttg atttcattgg ctgcccccac
    13141 atcgggatag ggtccaaaat aaagacctcc gtcctttttg acctgacgag tgataatcaa
    13201 gcgtggatag cgctcattgg tgattttgat gaaaggatag gacttgtcat ccttgagcat
    13261 gatattgtac ttgggcttgt tttccttgat caggttgatt tctaggagaa gtgcctcaat
    13321 attagactcc gtaacaataa attcaaaatc cacaatttca gacaccagag cctctgtctt
    13381 ggtatcatga cttccacgaa aataggaccg tactcgatta cgcagatttt tagcctttcc
    13441 tacatagata atggtgccat ttttatcctt atgaatgtag caaccagggc tggtcggcaa
    13501 gagctctagt tttgatttaa tcaagttgtt catagttcca ttatagcaaa aaaagtaggg
    13561 aaagatagta ccctactcac tagtctcata tatttttcgg aggtttgcaa catcctcttc
    13621 ctggatacca taaaaagaac ctgctggttg catgacagac ttctcatctg tatggtccaa
    13681 gccaatcgca tgacctaact catgttctgc cgtatggaca aggcgttcat aggagtagcc
    13741 atagtatgga ttggacaaat aataatgatt caaccgcacc gttacggaca agaattgccc
    13801 tgttaagaga ttagtttgac tttccgcctc tcctgccaca ggagtgcctc cgtcgttcat
    13861 ctccgtagcc gtaatatccg ccttactaga ctcagtcacg agttcaaagt taaaagcacc
    13921 agtttgattc cagttctgga ttgcttctaa ataagcctct tgaaagcgtg aatccatctg
    13981 cggatccagg taaatacgag cagaggcctg tgcccacctt ccttcagaat gctggtgact
    14041 atctgtctgg cttaacttag gcagttgccc tgttttctcc cattggtcaa tactttgttg
    14101 accaattttt actgaccgtt ctgcttgctt tagcgctcct tgaaaatctc cgttcagata
    14161 ccagagaagt ccgaaagcaa gcacacataa gagcacaact atccaaacca gacgccaaaa
    14221 caaacgccac acaaaagaaa agaaagcccc tatcaaacga aaaagccagc gcatgtctct
    14281 ccacctttct agcaaataag aattatttta ctaaaagaag ctaaaagaaa tctaaatact
    14341 gactttttca tcttaacgtc ctactatttt tttgagtaac tggatagctt tgtattttaa
    14401 aggagatgga tggtaacgga aagtacctgc tttgcgtaca atatagccat taaaattctg
    14461 tttaaaacgc aaaacaccat cacttccatc aaaaatccct tgaatgccta ggaagttgta
    14521 tttaggtatt ccacgtttta tgctttccaa cataacatat ttttgaagca gggcaggggc
    14581 atagaactta ttaaactcag tgtaggaacc actaaagaga taagtcgttt cctgaggcat
    14641 ataaacaaat aaactcccag ctaaaacaat atcttcttct ccatattttt caatcaagtc
    14701 tcgcgcttct gcttttcgaa cttcaaacgt ttcaaattga ctagaatatt ctctcagttg
    14761 attttgtttt ttctcagaat gaggattttt actcaaatca agtcgcaact tgtccaagtt
    14821 ttcttctagt ttactttgtt caccttgcaa tttgctcata tagtccgaaa aatttaagct
    14881 tgctatgaga aactccgctt gttctccaaa agtatcataa aaatgctcat aatattctaa
    14941 acttttatca ctatattctc tacgttcaga ggtttctttt gttatattct taaaaatcga
    15001 tagttcttca cgttttaact ttttcaaccg aatgccaaag gtttcagcct ttttcaccaa
    15061 gggtttaccc tttttgctaa aacttttaag caaactcttt tcagttaatt cagttaaatc
    15121 tttatagtat aaccaatctg gttctccacc tgggtaacct gttgttaagc catcaaattg
    15181 ataacctaaa tcagtcaaac cttgaataat actttttttc tcagcatcta ttggattacc
    15241 ttggctatca aaagtttgat aagtttcata aggttttaca agcaactcta atacaccatt
    15301 ttgcttggca tattctttta actctgcata aaaaactgga agagcatctt gttgggtata
    15361 aattggcccc gaattgagtt ccatatgcag accacccagc atgggcaagc tataaaccag
    15421 agctgcaact tgaatttctc cttcttgttt caaagcaaga taaacaattc gagcccctct
    15481 tttttctagc aaatccccca tctggacaga ttgcataaag gaacgagaag aaacctgatc
    15541 agaataagtc tgaaactctt ctttcgtgag tgttgttagt gccatatact tactttctat
    15601 gtttttttct taatgtttta cggaaatcaa gagcaagtct taacagagga tagagaggat
    15661 gagtgggcat tgtaaattca cccaagtatt cttcaatcgt tggattaaat ttttccttaa
    15721 aatgataaag tccaccattg agagagtttt caacaccacc taaattttgc cagaccatac
    15781 ctcgctcaaa ggcatagcga gccgtttcat accatgttaa aattggtgca ttgtaacgtt
    15841 taaaatcatc atccatacca gcatatatat taacagaggt agtaccaaat tccaaactca
    15901 aagtagccgc taaaggaact ctcgcttgac ctacatctat atattcctgc aagaaggtca
    15961 attcctctaa caaacgttct ttttccttct tctgcgcttc tacttttgaa gttcgagtcg
    16021 actcagtaaa cgtctcttcc aaggctctat ttttcgctaa ctgttcttct aattcttgcg
    16081 aacgtttaga aacatccaag gtggccaagg tgatataggc cttgtcctta aaattatcta
    16141 acaatttttt ataataggct tcattcctca aatgaatctc ttttcgcttc tcagtttttt
    16201 tcatcaactc cgaaaatgaa tctaatagtt ccagtccacc atattgaatc tcaagccctt
    16261 tgtttcgtgc tgttcgaata gcctgttttg ttgacttgga aagtttatct tcttcaaaat
    16321 tttccttgta tattttcgcc tgaatacgag gttgaatggt gtctcccatt tcctccgttt
    16381 ttcctgacca ccttactccc atttgttgca aactatcaat aatagccaga ttttcaggaa
    16441 attctgtctt ttcctgattg attaaacttt gagataggca aatacttggg tcaaaagtca
    16501 caaaaaccgc tctcttactg cgagcatagg acttaataga ctgaatggca aaattcaaga
    16561 gttctttatc cccataatcc aatataggtc ctcttgggat gtaaaacatt ttatagccta
    16621 gcggaagagt tctaatcaaa atactagctg tcgccagtaa tttttcttcc ctgtaaacac
    16681 caaacttctc atgttgccaa ttagacttaa cttcctccca agcactactt tgtaatacat
    16741 tggctaattc atgttctttg acaaactgat catattctaa tgtgggaatg ccaatttgat
    16801 aacggtacat ttcctactct ctttcctcca gtattttatt actattctac tactttcgtt
    16861 tgaaaagtct agtgaaaaca tattttattt tagttctact ccccctatgc tgaagttcaa
    16921 actaagtcag tctctcttct aatttgaaat caattggtag ggttgttaaa aaaacttcca
    16981 attgtttttc ccagtagtac cactcgtgag ttccagcact atggctatag gtcacatcaa
    17041 aacctagttt tttgagattt ttcactgcga gattattggc ttcgtacaag aaatcctgtt
    17101 cgccacacca cgcccaaagt ttggtctttt tatccgattt tttagccaga ctttcaagag
    17161 aatagggact agttgtccag tctctaatct ctccaaaaac acctctccag taggctggac
    17221 ttcccagatt ttgactttca ggagaaaagt tttgaaagct gagggcacct gaaaaactag
    17281 ctgcatgaga aaaacgattt gtcgtaagag ccagtttgaa gcagccgtag cctcccatag
    17341 aaagaccagc gataaaggtc ttttcacgct tgctagtcat attagggaag aagcgtttca
    17401 gaacctgtgg caattcctct gctagagcca tgtagtagtc aaaaccatac tgggtatcgg
    17461 tgtaccaacc attgctggta ttgggcataa caacgatgag attagttcct cgaagcaagc
    17521 gttctacatt ggtccgctta agccaactat tatgatttcc agacatcccg tgcaaaaggt
    17581 acaagacggg aatatcttca cactctggtt cttccactcg attggcatca gggtagagga
    17641 cattcacccc ccactccata tccaatactt gtgagtaata ctcgattttc attactgcca
    17701 tgattttctc cttctatttt tctataagaa aaagccgaaa ctcgactttc tctctgttta
    17761 tttcaacgat tattttgctt ccatgactac tggtaaaata gctggacgac gcttggtttg
    17821 gtcaaagaga tacttggtca aattatctcg aaccttccct ttaagatctg cccagtcaaa
    17881 gtcatctcct tgaagatact cttctaccgt ttggttaatc aattctgaac tttcacggag
    17941 aatatcgcga ctcttcttga gataaacaaa tccgcgcgta tgaacacgag ccctagccac
    18001 aattttcttc tcacgacggt tgactgtaat agccacgatg aaaattccat cctctgacaa
    18061 gaccttacgg tcacgaagaa caacatttcc aacatcacca atggcattcc catcaatcaa
    18121 gatatctcct gctgaaaccg atccagctgg aacaaagtct ccattctcgt aagccatggt
    18181 cgtccccttt ttaggaatga agatgcgttc tggcaacatc ccaactgcca tggcagcctt
    18241 agcgtgagca tccaactcac gatactcccc ttggacaggg aagaggtact taggttgcaa
    18301 aagattgatc atcagctgca aatcacgcac atttccgtgc cctgatacat gtaaactttg
    18361 ggtaatcaat ttgacaaccc cacctgcctg ataaatcata ttttccacac gcgcaacaaa
    18421 ggcttcttta gcaatagacg gagccgtagc aatatagacc aggtccccat ccttgatttc
    18481 tacataacga tggcgaccaa tcgacatctt acgaagtcca ttgataggtt cacccatacg
    18541 acctgtctca agaataatca actcatggtc ttcaaagcga gacatatctt taggcttaat
    18601 caagagaatt tcgttggcta aagacaactt cttaagacga atcgctgtgc ggacgatatt
    18661 ttcaatatca aatcctgtca agacgatacg tcgacctgtt ttatccgcag cgtcaaaaat
    18721 ctgctgaata cgagaaagat tactggaaac agctgcaacg atgatacgac cttcccagtc
    18781 agcaatagtt tgggtaattt catccctaac ttcactttca ctagccacct gaatattgct
    18841 gtctgcattg gccgaatcac tgaggagagc caggacgccg tcacgaccaa tctctgccaa
    18901 acgagcaaaa tcagttgcat aagattcact agccgtttgg tcaaatttga agtcacctgt
    18961 ataaacgatg cttccttccg atgtcttcaa gacaattccc agactctctg gaacggagta
    19021 agtcgtaggg aagaaggaaa ccactgtccc accaaaatca atctccgtat tctcatcaat
    19081 gacatggaaa tcattaaatt tcttaacggc atcatttcct ttgacaaaga gctttgccaa
    19141 ctcaatggtc aactcagacc caaatacagg aactttagcc tctgccaaga gataaggtag
    19201 agcaccaatg gcatccgcat gcccgtgggt caagaaaacc ccagcaatac ggtcgctatt
    19261 ttcaaaaagg taatccatgt ttggaatcac cacatcgacc cctaattgtt cattttcagg
    19321 atattttaac cctacattca aaacaaaaat ggactctcca atttcagcaa tgtacatatt
    19381 ttttccattc tcacgcacac caccaagtgt tgttaaactg atattactca tttttcctcc
    19441 aaaatcttag tttatcttga ttatagggca actctaccct cttattctaa aagttctaat
    19501 actttcagcc gaatcttttc ctaccattat accatttttt tgatgatttt caaaatgaag
    19561 gtctgttttc aaaaaagagt tgatttccca actctttcct aacttctact ctttttccat
    19621 caaaaaatca taaatttctt gcaccgtacc cagcgccatc atttcttggt ctttaacggt
    19681 ttgtttgaga ttttggtaaa tttgactttc tcctatctcc cgctttggcg cctctttatt
    19741 gactgtcatc acaccatctt ttatcgtcac aaagcctagt tcttcaaata cttgaatcat
    19801 cttgaccagc aagatttgtt gaatattaag atatgcagcc aaatctttca gcttgtagcg
    19861 aatatcaaac tctgggaact ggtaaatagt cttgtacaat ttggcaaact gatctctagt
    19921 cccataacct gtcagataat aggccttgtc aatatcattt ttgaaataga cagcagagaa
    19981 atgctgttcc tgaaaaatgg tcttcagctg agtaatatcc tctggaatgt tttttacgac
    20041 aacagcttca ctagccgcaa gatttggcag ttctccagga aaatccaaga ctggaacacc
    20101 ttctggcaag actgcatttt ttccacgaat gttaaaaagt tgaacacctt ccactcgcgc
    20161 atccatcatc atcaactgga gggcagtttg gccgttccat tggttgacag acaatttaac
    20221 cgctaactct agattcttgg tttgagaaaa ctctgtcgcc catctgcctt gaccaaaggc
    20281 taccacttca aaactcacct cacccttgga aattttcagc tttagatggg cattacctgc
    20341 ccccatagta cgagcacttt cgacctgaaa attcttgata taaaaaatag gtttctgatt
    20401 atccattcca aaaggagcta aacgttcaaa acttttgacc gtttccaagc taagtgcctc
    20461 caaatccaac tcttcatcta ggtttaactt attcttgcca ccagcatctg cacctttttc
    20521 acgaacataa tcttccaaaa tctgagataa atctgagagt tgctcaactt ccagcgtcat
    20581 acccgctgca cctgcatgac ctccaaaggc gatgaagagg tctcgatagg gatccagagc
    20641 ttcaaaaata tcgaccgctt ccacactacg agcactgccc ttggcacgac cgtcttctat
    20701 attaagaaca atgactgtct gtcccaattc ttccaataaa cgaccagcca cgattcctag
    20761 aaccccagga ttccagcctt ccttggccaa gacctgaacc ttcttctcag gatccaccat
    20821 ggtcttggct tcttcataga tagactgaac gatttccttg cgctcttcgt ttttctggtg
    20881 aatcataagg gcaatctcat gcgcttcctc atcatcaaat ccagtcaaca aatcaatggc
    20941 aggattggga tcatccaagc gacccaaggc attcaaacga ggagcaatct ggaaaccaac
    21001 cgtttcttct gttacttcgt tagcagcaat cccagccatg tccagcattt cttgcagacc
    21061 aatgcgctgg gtatgaccca acatttccag accatattga actaagatac gattttcatc
    21121 cgtcagactc accatatctg caatagttcc aatagcgacc aaatcaagca attccacttg
    21181 cacttcttct aacagggcac aagccaactt gaaagcaact ccacaaccag ccaaatattt
    21241 aaaaggataa tccgcatctg gatgttcagg atggacaata gcataagcat ctggcagggt
    21301 ttcaggtatg gaatgatggt ctgtcacaat gacatctact cccatagact gagccaatgc
    21361 aatagcctca tgaccagcaa ccccattgtc caccgtcaca atcaaggaaa ttccttcttg
    21421 ctcgataaag tatttataaa cactagcatt agggccatag ccatcggtaa aacgatttgg
    21481 caggtaaact cggcactccg caccaagttg ttccaaactt tccttcacaa tagaagccga
    21541 agtcatgcca tccgcatcat agtctccata aacgagaata ttttcccctt cttcaatagc
    21601 ctgacgaatc cgctccactg ccttgtccat atcatggagc agataagcat catgtaagtc
    21661 ctccaaggaa ggttctaaaa acttcttcag actttcttgg tcctgaatcc ctctctcaaa
    21721 caataaccga gccacctcag gacccagtcc agccttcttg gctatctttg taaaatccgc
    21781 attttctacc tgcagggcaa actgccattc ataagtaggt gttatcaaaa agacatccac
    21841 tctttctaag aattctattg cttcattata acatgattta gacgttttct ctatccagat
    21901 tgctttttta taacatttta tagtatattg aaactagaat agtacacctc tacttctaaa
    21961 atattgttag aaatcgattt gattgtcccg atcgattttt cctgttctta tatcactata
    22021 gtgaattttt tcagatatga tttgacaaga acattctttt atcgtagaat catgttataa
    22081 aatctaacac aagggagatc tctcatggct ttagtagaat ttaaaaacgt cgaaaaatat
    22141 tacggagact accacgcact ccgcaacatc aatctccgtt ttgaaaaaag gacaagttgt
    22201 tgtcctgctt ggaccttctg gctctgggaa gtccactctt atccgtacga tcaatggttt
    22261 agaggctgtt aacaaaggaa gtctcctagt caatgggcac caagttgctg gtgccagcca
    22321 gaaagatttg gtacctcttc gcaaggaagt cggcatggtt tttcaacatt ttaaccttta
    22381 tccacacaaa acagtgttag aaaacgtaac acttgcaccc attgaagttc taggaattga
    22441 taaaaaagaa gctgaaaaaa ccgcccaaaa atatctggaa tttgtaaata tgtgggacaa
    22501 gaaagattcc tatcccgcca tgctatctgg tggacaaaaa cagcggatcg ccatcgctcg
    22561 tggtcttgct atgcatccgg aactcctcct ctttgatgaa ccaacatctg ctcttgatcc
    22621 tgagactatc ggagatgttc tagcagttat gcagaaactg gcgcatgatg ggatgaacat
    22681 gatcatcgtt acccacgaaa tgggctttgc tcgagaggtt gcggaccgca ttatctttat
    22741 ggccgacgga gaagttttag tagatatgac agatgtcgat agcttttttg acaatccaag
    22801 cgaacctcgt gcccaacaat tcctcagcaa aattatcaac cacgaaagtg acaaagtcaa
    22861 ataaggaggc gcctatgaaa aaaaattctt tttatcagca ttattgatta gccttttcgg
    22921 ccttgttgct gccaaaccag tccaggctaa tactagtatc gcagacattc aaaaaagagg
    22981 cgaactggtt gtcggcgtca aacaagacgt tcccaatttt ggttacaaag atcccaagac
    23041 cggtacttat tctggtatcg aaaccgactt ggccaagatg gtagctgatg aactcaaggt
    23101 caagattcgc tatgtgccgg ttacagcaca aacccgcggc ccccttctag acaatgaaca
    23161 ggtcgatatg gatatcgcga cctttaccat cacggacgaa cgcaaaaaac tctacaactt
    23221 taccagtccc tactacacag acgcttctgg atttttggtc aataaatctg ccaaaatcaa
    23281 aaagattgag gacctaaacg gcaaaaccat cggagtcgcc caaggctcca tcacccaacg
    23341 cctgattact gaactgggca aaaagaaagg tctaaccttt aaatttgtcg aacttggttc
    23401 ctacccagaa ttgattactt cccttcacgc tcatcgtatt gatgcctttt ccgttgaccg
    23461 ctctattctg tctggctaca ctagtaaacg cacagaacta ctagatgata gtttcaagcc
    23521 atctgactac ggtattgtta ccaagaaatc aaatacagag ctcaacgact atcttgataa
    23581 cttggttact aaatggagca aggatggtag tttgcagaaa ctttatgacc gttacaagct
    23641 caaaccatct agccatactg cagattaagg aggacacccc atgacagatt tatcatcttg
    23701 gacagcctat tttcaggatt ttggacaatt tttcaatggt ttcctcttta ctcttgccct
    23761 agcgattgga tcctttatcc tcgccatggt cttaggaatc ttctttggtg ccatgtcaac
    23821 cagtaaacgt ccaattttac gcattttagc tcgcatcttt gttgaatttt accaaaatac
    23881 tcccctcttg gtgcagtttg ttattgtttt ttatggccta cctcttatca gtgaccatat
    23941 catcatgatt ccaatttatt ggacagccgt tctctgcgtg ggactctatc acggcgctta
    24001 tatcgctgag gttattcgtt cagggattca gtctattcct agtggtcaga tggaggccgc
    24061 cttgtcgcaa ggttttacct atatcagtgc catgcgcttg attaccttgc ctcaggcctt
    24121 ctgcatcatt ctacctccat tgaccaacca aattgttaac ctcatcaaga acacctctac
    24181 tgtagctatc atctctggag tagacttgat gtttgtgact aagtcttggt cggctctcaa
    24241 cggaaactat atcccagcct ttttaggtgc tgctcttctc tactttgccc tatgcttccc
    24301 tgttgcccag tttggtcgca agatggagca agccaacaaa aaagcctatt cactttagga
    24361 ggttactatg gaatctattt tagaagtttt aaccccagat aacctagtct ttatctttaa
    24421 aggatttggc ttgaccctct atatttctct gattgccatc atcctctcta ctatcatcgg
    24481 tacggtgcta gctgtcatga gaaatggcaa aaatcctatc ttacgcatta tttccagtat
    24541 ttatatcgag tttgtgcgca acgttcccaa ccttctctgg atttttacta tctttttggt
    24601 gttcaaaata aagtccacac cagcaggtat tacagccttt actctcttta catcagcagc
    24661 cttggctgag attattcgag gcggtctcaa tgccgtagac aagggacaat acgaagcagg
    24721 aatgtcacaa ggcttcacct cagcccaaat cctctactac atcattctcc cacaagccat
    24781 ccgcaaaatg ctaccagcca tcatttctca gtttgttccc gtgattaagg ataccagtct
    24841 cctctactct gttatcgccc tacaagaact ctttggagcc agccaaattc tcatgggccg
    24901 ttatttcgaa ccagagcagg tcttcagtct ttacatcctg attgccctca tctacttcag
    24961 ctttaaccta gcaatttcta gcctgtctca tatgctagcc aaacgttggc aacaagctgc
    25021 agaataatac tcttcgaaaa tctcttcaaa ccacgtctgc ttcaccttgc cgtatatatg
    25081 ttactgactt cgtcagttct atccacaacc tcaaaacggt gttttgagca gcctgcggct
    25141 agcttcctag tttgctcttt gattttcatt gagtataaac agccactctc cagttcaatt
    25201 ggagagtttt tttgatgaga gtagatattt atagacctca atttgacacc atacattttc
    25261 tatgataaaa tgtaacaaat ttatttacaa caaatcgaaa tctaaatgaa atggaatttt
    25321 ctatgaaatc aatcaaagga ctactcttta tcatagctag ttttatcttg actcttttga
    25381 cttggatgaa cacttctccc caattcatga ttccaggact agctttaaca agcctatctc
    25441 tgacttttat cctagccact cgtctcccac tactagaaag ctggtttcac ggtttggaga
    25501 aggtctacac cgtccacaaa ttcacagcct ttctctccat catcctacta atctttcata
    25561 actttagtat gggcggtttg tggggctctc gcttagctgc tcagtttggc aatcttgcca
    25621 tctatatctt tgccagcatc atccttgtcg cctatttagg caaatacatc caatacgaag
    25681 cttggcgatg gattcaccgc ctggtttacc tagcctatat tttaggactc tttcacatct
    25741 acatgataat gggcaatcgt ctccttacat ttaatcttct aagttttctt gttggtagct
    25801 atgccctttt aggcttacta gctggttttt atatcatttt tctatatcaa aagatttcct
    25861 tcccctatct agggaaaatt acccatctca aacgcttaaa tcacgatact agagaaattc
    25921 aaatccatct tagcagacct ttcaactatc aatcaggaca atttgccttt ctaaagattt
    25981 tccaagaagg ctttgaaagt gctccgcatc ccttttctat ctcaggaggt catggtcaaa
    26041 ctctttactt tactgttaaa aattcaggcg accataccaa gaatatctat gataatcttc
    26101 aagccggcag caaagtaacc ctagacagag cttacggaca catgatcata gaagaaggac
    26161 gagaaaatca ggtttggatt gctggaggta ttgggatcac ccccttcatc tcttacatcc
    26221 gtgaacatcc tattttagat aaacgggttc acttctacta tagcttccgt ggagaagaaa
    26281 atgcagtcta ccttgattta ctccgtgact atgctcagaa aaatcccaat tttgaactcc
    26341 atctaatcga cagtacgaaa gacggctatc ttaattttga acaaaaagaa gtgcccgaac
    26401 atgcaaccgt ctatatgtgt agtcctattt ctatgatgaa ggcacttgcc aaacagatta
    26461 agaaacaaaa tccaaaaaca gagcttattt acgaaggatt caagttcaaa taagtaatca
    26521 tactctcctg ggaaatccca ggagagtttt ttatagccaa atgaaataag acaggaacga
    26581 tggaattttg gcattcaaat caattgataa gactatttta tagtggagtg aaataggata
    26641 cgaacaaatt gattaggaaa atcaaatgaa tttatagaaa tcttttagca gttatgttat
    26701 cctattctag tttcaaaacg ctatagaagc agcattgtac tagtctagat tcagtttact
    26761 atactaaaac gagtagcttg aaatcaaaaa acccaccctc acaggcaggt tttatctgta
    26821 ttattcagct agattatgct ttaccttctg aaccgaatac gtcgatacgt tcttcaaccg
    26881 atgcttggat agcttttaca ccgtcagcca agaatttacg tgggtcgaag agttttttct
    26941 tgtcgtattc tgcttcgttt gcttcgtagt cacgagcaaa tttacgagtt gcgttagcga
    27001 atgcgatttg gcattctgtg ttaacgttaa ctttggcaac accaagtttg atagctgctt
    27061 ggatttgctc atcaggaata cctgatccac cgtgcaatac gattgggaat cctggaagag
    27121 cttctgtcaa tttttgcaag tggtcaaggt caagaccttc ccagtttact gggtaaggac
    27181 cgtggatgtt accgatacca gctgccaaga agtcgatacc agtttcaacc attgctttag
    27241 cgtcttcgat tggagccaat tcacctttac cgatgattcc gtcttcttca ccaccgatag
    27301 taccaacttc agcttctact gagatacctt tagcgtgtgc tttttcaaca acttctttag
    27361 ccaatttaag gttttcttca actggaaggt gtgaaccgtc aaacatgatt gaagtataac
    27421 caacttcgat acactcaagt gcatcttcgt agtgaccgtg gtcaaggtgg atagctactg
    27481 gtacagtgat acccattgat tcaacaaggt tagcgatcaa gttgcgagca actttgtaac
    27541 cacccatgta tttagcagca cccattgaag tttggatcaa aactggagct tttttagctt
    27601 ctgctgcgcg caagatagct tgagtccact caaggttgtt tgtgttaaat ccaccaactg
    27661 cataaccgtt gtcacgggct gcttggacaa atttttctgc tgaaacgatt gccattttat
    27721 caggcctcct gtatattttt atgggtcatc ccatttacat tgttcatttt atcacttttt
    27781 gccaaaaaaa tctagttttt cccgcagttt cgattgattt tcttctaact ccatctatgt
    27841 aaaccctttc tctccctagt cttgggcgac ttttggaaaa gctataaaga aggttaaacg
    27901 attctcctcc atctcgaaac gataagctaa tttttcatgt tctaatagac tcttaaccac
    27961 aaagagcccc ataccagacc ccttgacctt gcgactggca ttgtcagaaa aagactgggc
    28021 tagtttttct tgttcctctg agctacagct attttcgata aaaagttctc cttctctttc
    28081 tccaattcga actaagccac ctggaacaga gtgcttaatg gcattgctga tgagattaga
    28141 aagaatcaac ttcataactg atgggtttag ataagcctgc tgatgggtca aactattgtc
    28201 tatctggagc tctctttcct tggctagcaa ggcataatct ttgaccagat tttgcgtcat
    28261 ctggaggagg tcaattgttt ccctatcatc tcgcaattcc tgcacagaag agagggaaag
    28321 tatctgcaga acatggtgat tgagttcatc cacaatcccc aaggcaactc ccagatactg
    28381 gtctctatcc ttataacgac cgatattctc tctcatattt tcgattagga ttttcaaact
    28441 agccagcggt gttttcaatt catgagaagc tcctcgtagg aattcgacct tcatcttctc
    28501 cagctggaga atggcttcat tcttttcatg caagtccgca ataacagtca agagatgctg
    28561 gtagaggcta ttgatttgtt ccttgagatt acctatctca tccttagaat ccacgcgcaa
    28621 tcgcacttgg gaatccaggt ccatcatccg acgggtcacc cgcttgattt ccaaaatcgg
    28681 tgcaacaata gtccgggcgt agatgtaggc caccaagagg gaaatcagaa aggaggccag
    28741 caaggtatag ggaagaaact ggagactgat ttgctccgct tccttttgta aatccatgga
    28801 agctagaaac tggagaatca tagtaccacc gtcttgcgtt ttcacctcgc gctcctcaat
    28861 aaagagagag gttgtctggc ggtctgtgtc cagaggaaga ctgtccttga cttctaactt
    28921 gtcctcggtc atctcacctt tgacggtccc cttgatatca ctagtctggg aatacaagtc
    28981 taacacttgc tcgatactct gcctatcttt cccttctagg gactgggcaa tggctgttgc
    29041 cttttgacca atggtttcct gacgatgact cagataagtc gaaggaaaaa gaaaataaat
    29101 agctaaatga aggcagataa ccagaacact aaatatcgag aaggtataga taaatatctt
    29161 tgcaaataaa cctgttcgtt tcattttcgc tccaatttat aaccaacatt gcgcacagtg
    29221 aggatacaat ccaagtctag ctttttccgc aattccttga tataaacatc aataacacgg
    29281 tcaaagggaa cctcatctgt cgctttccag acggcatcga taatctgaga ccgagtcaag
    29341 gcccggcctt catttttcac tagatagtcc agaatttcca actctttggc attgataggc
    29401 acttcttgac ctgcgaggct tgcactgtag ctttcaaagt ccaccttggt atccttgtaa
    29461 gaaaagattc gtcctgtatc gtagtagcgc ttgaaaatcg cgtccaccct cacttttaag
    29521 agggagagag agaaaggttt ttccagatag ccatctgcca aagaggcaaa ggcactcatc
    29581 ttgtattcct catcctgaaa agctgtcaac atcaagacag gaacctgact ggttttacga
    29641 atctcagcta ggacttctaa gccgttaagc ttgggcatct ggatatccag taaaaccagg
    29701 gccacctcat agctagaaaa ttgctccaga gcttcctgac cgtccgctgc ctcaatagtt
    29761 tcatagccac aatccgtcaa ataatcactg accccctcac ggatcatctc ttcatcttct
    29821 acaattaaaa ttttcatact ttaactgctc tctatttttt atttttctta gaataaatac
    29881 ctactctatt ttctattata gtctcttgct ggccttttgt ctgtaagcaa ctgatcacta
    29941 gataaaacgt tgtgaaattc ctttctcata aattccataa ctttagtata ttatatttaa
    30001 gcactaaagt acaaagaaag caactgaaag caatgatttt caccactgct ttcagattta
    30061 ttttgaattg ttaaatagct attcctatcc actattcttg aatagaaaca caagatgcaa
    30121 tctttattcc agactcattt tttcaaattt attcaccatc cagcaagagc tcttttggtt
    30181 gttttctaag gagattgctt gaagcaagcg ccataacgag aaccactaga accaaggcaa
    30241 ggacaaaaat gatgataaag tctgatgtct gaatggaaat gtctaggctc gacaaggtct
    30301 tgctaaagcc atctacttct gcaccgccac caaggttaga ggcttgagcc gccttactag
    30361 cctgtttggc aacacctgaa gtcacattgg caaggacagt gtttccaatt gcacgggcag
    30421 tgtaattagc taggaagtaa gcagaaacta gagcagggat agcaatcaag atagattcgg
    30481 tgatgaattg acccaagata cttgcctgct tgaggccgat agagaggaga attcccactt
    30541 ccttgcgacg ggcgttgatc caaaggctga gcaagagggc aaggaggaga actgagaagc
    30601 tcaagctacc ccagaagagg aggttggcca tcttgtacat accagagata gattgctcaa
    30661 gagctgggta gttagaggag ctcttgacga gtgtgtagct cttccagttg ataccactga
    30721 tgccattcaa ctctttcata acatcatcca agttcttgtc tgctgttaca aagaaggttg
    30781 cgtccccata aatggctgtg tcttctgtgt atccataaag ttttgcagca gtgtgaatgt
    30841 ctgtaatagc tgtgttttcg taaagttctt gtgagtaggt tactgctgac ttattatgac
    30901 catcaaagag tcccttgatt gtcacttcaa ctgtttcctt ggctcctttt tcattatctg
    30961 catcgtagat attagagtcc agtttaacct tgtcccctac tttccagccg tgtttggctg
    31021 ccaagtcctt gtgcaagagg attttatcct tgtcgtcgtt ggttaagtgc tctccttcga
    31081 ctagtttata agaaccagag acaaacttat cttctttaga ggagtcattg acacctgtaa
    31141 tcatcaagct acttccaaaa cgcttggcac gatcagcagt gagattcttc ttggtttctg
    31201 gcgtttcaat caggtcatat ccagtcaaat ctccgatagc gttgatacgt ttgacataag
    31261 actcgatggc cttgttttcg gtgatttttt tgatgtcttc acccttgata ttcccagcac
    31321 cacgaggcgt tccttggttg acgcgacgat tgatttgcat ggagaagcta ttggtgatat
    31381 ttttaaaggt ctcctgagaa gccttggcag tagctccctt gattgacaag ccgaccaaac
    31441 tcaagctcgc catgaggaga ataatcagga agatgacaat cgatttgaaa aacttccttg
    31501 taacataggc aaatgcgttg tgtaacatag attccctttc tagattttgt tttaatcatt
    31561 ctatcaaaat aagctcaaat tatttactag tattgcgcgt ttcagtcagt ttcttatcct
    31621 ttaattcaag tgtaatatct gacgcttgtg ccacttcttt actgtgagtt acgacaatca
    31681 cacatttacc tgttttctgg gcaagtgatt tgagtagttc gacaatatct ccagcagttt
    31741 taggatccag atttcctgtt ggctcatcag ctagaataac tggagcttct gagaccaaac
    31801 tgcgagcaat ggcaacacgt tgctgttgac cacctgataa ctggagaaca ttccgcttga
    31861 tctggctttc atccaaacca agctcaagaa gtgtattctt gcttgccttt ttgttgacca
    31921 atcggatatt ttccagcgga gaaagataat ctatcaagtt ataattttga aagaccaggg
    31981 aaatatggtg catgcgatgg taagaatagc ccttcttacg aatatcctct ccttgaaaaa
    32041 ggatagaacc ttcaacagga ctatctagac cagcaagtag ggacaagagt gtggattttc
    32101 ctgctcctga ctccccaata atactgtaaa attttccggg ttcaaaatta taatcgatct
    32161 gatataggac tgcttcagca gtattcttat aacggtaggt aacatcttgt aattgtaata
    32221 aagtcatgat ttctccttct taactaatag atgataaaat ttctttcggt gattttctaa
    32281 ataagaatag gaaacaaagg gctacagata agcaactaag cagaactaga aaaacatagg
    32341 attctgcaaa agataagata ctagttgata aactgcttgc tttggctagt gtatcttgta
    32401 agcttgcctg atctccactt gctagtagag tttggagtag gtaagttgtg attccgtttc
    32461 ctgcaacaaa tgctggaagc aaagctccaa gagataccaa aactacctct aaacagaatt
    32521 gtaggaagat cgagctcttg ccttttccaa gtgcaagtaa aatccccact tcatagaccc
    32581 gttctctcaa ccagagagac aaaaccagaa ttaaggctcc agctcctgct atcaacatcc
    32641 cataaaggaa gatggtcagg aaggtttgga aagttgcaac tgagtctttg atttgttcaa
    32701 aagccttgtt ttccttttcg acttggtagc cttgattttc caaggccaag ttttctacct
    32761 gcttcatgag tccgtccatt tccttaggat tttctacata gaagcgtgct gcactgactt
    32821 gagcttcact attgcccaaa agggtttggc tactttcata gtctgtaaag acttgatttt
    32881 cactgaagtc agaagacaag cctgtgaatt tctcttgttt tttaccagaa aagatgccga
    32941 taatctcaaa ctctactgtt tgtccttttc cagattcaga ctgaccagca tccaagccaa
    33001 tcttgtcatg aagtgaaaga ccgttcttct tagccaattc ttcgtggata aggattttct
    33061 tggaatcccc tttttgaagg tgtcgccctt cttttagatt gaaagccgaa ctggtaaagg
    33121 ttacatcctt ggatgaatcc tcaagagccg ttaagctaac caagttattg tctgcagctg
    33181 ataaatcatc acgctccacg ctctgctcgc cagtcactgc ttccttgtct tttagttttg
    33241 cgaccgtctc aagttcagga gagacatttt ccagcccctt aatcttgctt acagatgcta
    33301 ggtctgacaa cttgaatgtc tgaccattct ctatcttctt aatagaaaaa gatgtattga
    33361 gtgatttata aagattgctt tctactgttt tgttggactt catcagagtc aaacaggctg
    33421 aaattccggc caataagacc aataaaatca gaaataaaat aaaacttctc agtcgctttc
    33481 tgctgacata agcccaagat ctttggattg gattcatttg tcacctccat atttgtaaga
    33541 ctattataaa acccaaatat gaaatgttta tgaaatacga aaaaaaaata tcgagtaggg
    33601 gataatctct agcccctctc acaccaccgt acgtgccgtt cggcatacgg c
//
LOCUS       DAVLWA010000015        33452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLWA010000015 DAVLWA010000000
VERSION     DAVLWA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.015"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(667..1002)
                     /locus_tag="VYN80_000682"
     CDS             complement(667..1002)
                     /locus_tag="VYN80_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540632.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1093..2403)
                     /gene="der"
                     /locus_tag="VYN80_000683"
     CDS             complement(1093..2403)
                     /gene="der"
                     /locus_tag="VYN80_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1540633.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2417..3130)
                     /locus_tag="VYN80_000684"
     CDS             complement(2417..3130)
                     /locus_tag="VYN80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1540634.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3127..4023)
                     /gene="dnaI"
                     /locus_tag="VYN80_000685"
     CDS             complement(3127..4023)
                     /gene="dnaI"
                     /locus_tag="VYN80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1540635.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4024..5193)
                     /locus_tag="VYN80_000686"
     CDS             complement(4024..5193)
                     /locus_tag="VYN80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1540636.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEYPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5194..5667)
                     /gene="nrdR"
                     /locus_tag="VYN80_000687"
     CDS             complement(5194..5667)
                     /gene="nrdR"
                     /locus_tag="VYN80_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1540637.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5807..6172
                     /locus_tag="VYN80_000688"
     CDS             5807..6172
                     /locus_tag="VYN80_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1540638.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6177..6872
                     /locus_tag="VYN80_000689"
     CDS             6177..6872
                     /locus_tag="VYN80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540639.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6889..7656
                     /locus_tag="VYN80_000690"
     CDS             6889..7656
                     /locus_tag="VYN80_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540640.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(8009..9208)
                     /locus_tag="VYN80_000691"
     CDS             complement(8009..9208)
                     /locus_tag="VYN80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540641.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9205..10098)
                     /locus_tag="VYN80_000692"
     CDS             complement(9205..10098)
                     /locus_tag="VYN80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540642.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10214..10294
                     /locus_tag="VYN80_000693"
                     /pseudo
     CDS             10214..10294
                     /locus_tag="VYN80_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10779..10992
                     /locus_tag="VYN80_000694"
                     /pseudo
     CDS             10779..10992
                     /locus_tag="VYN80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11407..12024
                     /locus_tag="VYN80_000695"
     CDS             11407..12024
                     /locus_tag="VYN80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1540643.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(12344..13231)
                     /locus_tag="VYN80_000696"
     CDS             complement(12344..13231)
                     /locus_tag="VYN80_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1540644.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(13380..15317)
                     /locus_tag="VYN80_000697"
     CDS             complement(13380..15317)
                     /locus_tag="VYN80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEV1540645.1"
                     /translation="MDYSKVAAEVIGAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGDKVKVGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            15693..17147
                     /locus_tag="VYN80_000698"
     CDS             15693..17147
                     /locus_tag="VYN80_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1540646.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            17128..18093
                     /locus_tag="VYN80_000699"
     CDS             17128..18093
                     /locus_tag="VYN80_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1540647.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(18128..19402)
                     /locus_tag="VYN80_000700"
     CDS             complement(18128..19402)
                     /locus_tag="VYN80_000700"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1540648.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(19402..20574)
                     /locus_tag="VYN80_000701"
     CDS             complement(19402..20574)
                     /locus_tag="VYN80_000701"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1540649.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETKSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20705..20953)
                     /locus_tag="VYN80_000702"
     CDS             complement(20705..20953)
                     /locus_tag="VYN80_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540650.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIDELSSN"
     gene            complement(21021..21647)
                     /locus_tag="VYN80_000703"
                     /pseudo
     CDS             complement(21021..21647)
                     /locus_tag="VYN80_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21709..22158)
                     /locus_tag="VYN80_000704"
     CDS             complement(21709..22158)
                     /locus_tag="VYN80_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1540651.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(22160..22918)
                     /locus_tag="VYN80_000705"
     CDS             complement(22160..22918)
                     /locus_tag="VYN80_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1540652.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(23033..25012)
                     /gene="pknB"
                     /locus_tag="VYN80_000706"
     CDS             complement(23033..25012)
                     /gene="pknB"
                     /locus_tag="VYN80_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1540653.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKAQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(25009..25749)
                     /locus_tag="VYN80_000707"
     CDS             complement(25009..25749)
                     /locus_tag="VYN80_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1540654.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIVDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(25764..27077)
                     /gene="rsmB"
                     /locus_tag="VYN80_000708"
     CDS             complement(25764..27077)
                     /gene="rsmB"
                     /locus_tag="VYN80_000708"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1540655.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(27070..28005)
                     /locus_tag="VYN80_000709"
     CDS             complement(27070..28005)
                     /locus_tag="VYN80_000709"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1540656.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(28018..30414)
                     /locus_tag="VYN80_000710"
     CDS             complement(28018..30414)
                     /locus_tag="VYN80_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1540657.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(30480..30794)
                     /locus_tag="VYN80_000711"
     CDS             complement(30480..30794)
                     /locus_tag="VYN80_000711"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1540658.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(30819..31445)
                     /gene="gmk"
                     /locus_tag="VYN80_000712"
     CDS             complement(30819..31445)
                     /gene="gmk"
                     /locus_tag="VYN80_000712"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1540659.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(31575..33188)
                     /locus_tag="VYN80_000713"
     CDS             complement(31575..33188)
                     /locus_tag="VYN80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1540660.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         9454 a         7604 c         6426 g         9968 t
ORIGIN      
        1 tgtgaacgaa tcaattaggg gatctaaagc attttatacc aatgttttat aagttgtatt
       61 gtattattcc atattcaatc cactatacaa gatgaatatt tctctcaagt ttcataatca
      121 aaataaaaat gactgtatct atagctccat aaaaaaatca tagatacagt cattttttag
      181 ttcctacacc ttcctttatg cacaccttta gacttcccta gttcccgtct caagtcaagc
      241 atttttttga aacagatata gtctaaccag tcactagcaa aagcgataag tagtaataat
      301 agccaaacac cccaagactt gatgtctgcc acatttctca agacgatatt gaacaacagg
      361 accgaaaaaa ctgtccaagc cagactaaaa agagcataga gggtgatgta aatccagtaa
      421 aaatagtaaa aaattgagaa aatcttacta tctctgtttg ccctttcaat ccagctgaaa
      481 aagtaaaaac agggtaataa gattaccatt ttaaacaagt gttccatcac aaacctcctc
      541 ttttaaattg ttttctttag tttcgttcta ttaaatgtta ttaccaatat aaaccttgga
      601 atagcgtgta ataaatgatt ttagaggtac cataagccac ctcctacaaa tagaaaccga
      661 tataaatcaa tgccttccac ccttagactt ccctagttcc tgtctcaagc gaaacatttc
      721 tttgaaacag gaataagtta accaattcat accaatagct agcagaataa aaagaaacca
      781 aatgccccat aacttgatat ctgtcacatt tctcaagacg gtattgaaaa acagaactga
      841 aacaactgtc caagcaaggc taaaaagaga atagaagggg atgtaaaacc agtaaaaata
      901 ataaaaaatt ggaaaaaact tactatttct gttggccttc tcaatccagt tatcaaaata
      961 aaagtacggt gctaaaagta agaatttaaa caaatgttcc atcaccgaca tcaccctcct
     1021 tcttttgata gcgttttcta ttattttatt atatcaaaaa aatccggaac tgtcattcca
     1081 gattctactt ttttatttgc gttttcttgc gatgagatga atcggtgttc cttcaaaaac
     1141 aaaggccttg cggatttgat tttccaagaa acgcaggtaa gaaaagtgca tgagttcttc
     1201 ttcattgaca aagatgacaa aggttggtgg tttggttgcc acttgggtcg catagaaaat
     1261 cttgagacgt tttcctttgt ctgtcggtgt tgggttgatg gcaatggcat ccatgatgac
     1321 atcgttcaag acagctgatg gaatacgtgt attttgactt tcgctgattt gcttaatcat
     1381 ctcaggaagt ttgtggagac gttgcttggt taaagctgat acaaagataa tcggtgcgta
     1441 aggcaggtat tggaactgct cacggatatc ttcttcccag tttttcatag tgtggttatc
     1501 tttttcaagc gtatcccact tgttgaccac gataatcatc cctttaccag cttcatgggc
     1561 aaatcctgcg atacgcttgt cgtactcacg aatgccttct tccgcattga tgaccatcaa
     1621 gaccacatct gaacggtcaa tagcacgcat ggcacgcata acagagtatt tctcagtatt
     1681 ttcataaacc ttaccagact tacgcatacc agccgtatca atcatggtaa actcttgacc
     1741 atctgtatct gtaaagtggg tatcaatggc atcacgagtt gttccagcaa caggactagc
     1801 aataacacgg tcttcgccca agatagcatt gatcaagctt gattttccaa cgtttggacg
     1861 accaatcagg ctaaacttaa tgacatctgg attttcttct tcatattcat ttggaaggtt
     1921 ttccacaatc gcatctagta catcccctgt accgatacca tggacagatg agataggcag
     1981 tggttcaccc aaaccgaggg catagaaatc atagatatca tttcgcatct cagggttgtc
     2041 taccttgtta actgcgagga taactggttt gtgggtctta taaagcttac gggctacgta
     2101 ttcgtctgca tcggtaattc cttccttacc ggacacgaca aagacgataa catctgcttc
     2161 ttccatggca atttctgcct ggtgcttgat ttgttccatg aaaggagcat caacatcatc
     2221 aattcctcct gtatcaatca tgctaaaaga acgattgagc cactcacccg ttgcataaat
     2281 acggtcacgt gtcactcctt cgacatcttc tacaatggag attcgctcac cagcgatccg
     2341 attaaatagg gttgatttcc caacattggg acgtcctaca atggcaatag ttggtagggc
     2401 cataatttct cactttctac aataatttct tctgttcaag attttttcta gttgagcttg
     2461 gttcagcttg accaaactgt tctgctagac gctgactcca gcttgtggtc gcacgcgccc
     2521 cagcatagtc agcctgaaca cggtcataag cttggattgc ctcagttgac tgttcttggt
     2581 attcttcctc aaagacaaca ttctctagtg gcagtctcgg tttcatatca tgatgttgat
     2641 ttggcacacc cagtgccatc ccaaagacag aataggtgta gtcaggtagg ttaaagagct
     2701 ctgccacttc ttcagacttg tatcgaacca aaccgataat cacaccacca tagcccaagc
     2761 tttcagctgc caacaaggca ttttgtccag caagagctgc atcgaccgaa ctaatcaaga
     2821 gaccttccac accttggggt tggaaggtgt cggtatgaag tcgggctccc ttttctgctc
     2881 ggttcaaatc tccgacaaag agaaggaaaa cagcagactg gcgaatggct tcttgaggta
     2941 ccaattcata caaggcatct ttcttctctt gacttcgtac cacaatcaca gagtaggatt
     3001 ggaaattctt ccaagatgat gccatctggg ctgctgtcaa aatctcattt aagtctactt
     3061 ggggaatttc ttgctcttta aacctgcgca ctgaagtatg agccttcatc aatttaatgg
     3121 tttctgtcat cgacggttta ctccttctaa acgagtctcc tcagccaaat aacggatgcg
     3181 ttccatgacc cgtctggctt cccaggtttc gtcatttcca tgtttcactt tcgcaaaatg
     3241 cttctccaaa tcttcaaagt tgaagttgga tgtgaaaaag gtcggtaaat tttcctgcat
     3301 ccgatattgg agaatgacct gcaggatttc gtcacgcacc caaacggttg attgctcggc
     3361 gccaatatca tctaaaatca ggacctcaga cagcttaatc tcatccacca aggtcttaac
     3421 attgccatca ctgatagcat ttttgacatc aatgacaaag ctaggatagt ggaggagagt
     3481 tgatgaaaca ccacgttttt ctgataaatc atgagctaag gccgccacca tgaaactttt
     3541 acccacacca aagtctccat ataagtaaag accttttcga atagctggat attgctccac
     3601 gaaggctagt agcttttcaa aaactggtaa gcgccccaaa tcatccaagt caacttgagc
     3661 caaactagct ttcttgagac tggctggtag attgattaac ttgagacggt tcttaatagc
     3721 cgcttctttt tcagccgcga ttagctcagg agtttcttca tatgaaacat ctgcatagcc
     3781 atgattctta accaaaatcg gcttgtagcc tttggcaata taatccgtat ccccacggag
     3841 aaacttgtca cgctcggtga tgtactgatt aaacttggag atactgcgat ttaattcctc
     3901 tggagtcaag gattcttgct ggataaaagc cgcaacatca gggtccttca tgattttctg
     3961 gaccaaatct tgataataaa aacggctggg ttgacgtttg agtacgtctc cgacactttc
     4021 catctaatct cctccttttt ctaatcgagc taatagttct tgcttcttac gttctagttc
     4081 cagacgagtt tcctcgctgg tttcattctt atattcagga ttactccatt taggaacatt
     4141 ggtttttact ggggcagtct gattctgttt ttgtgttttt gctttctgtc ctcgctcacg
     4201 gatgcgcaag actgcctttt ctgccgaatg aatcttttga taggcatagt cattggctac
     4261 cttcatggca tatttctcat tgatatttgc cgaatcgacc ttattaaagg tcaataaaag
     4321 aatgatattg atgacttcgt ccagcaagcc caagccagcc atctgttgca agagttctct
     4381 ttctgtttgg gtaatggttc ccttgcgtgt ttgcttgatt tctgccaaga actgcagggc
     4441 agttttactt ttggcttctt tgataatggt tgtttcctta agactaaagt cagaggaaac
     4501 tggtttttga gcaatttttt cacgcatgcg tttggttgaa ataacctgag aaacagctgt
     4561 tgacttggcc aattgatagg tttcaaacca agtccatttc ttctcctcgg caatagcaaa
     4621 gaggtttaag acatcggact gctcatccgc aaaacgaagt ccatctcgag ccatcagctg
     4681 gcgaaaatgt tccaagtcaa aatcattggc cactttcttc ttgagaccaa ggtcttcttg
     4741 actgcctagt tctgccaatt ctggaaagac ttgattgagt gagacaggta tttcttcacc
     4801 atcagcactt tcaactttca aatcctccac agcagagtcg ccaatctttt tctctaagag
     4861 tctgcgataa acaggatact ccaagaagtc ttgactagat agaggagcat ggagggctag
     4921 ctgataaaca tccccctttt gatagagggt caagagatta aaagcagata agattttcaa
     4981 tgattttatc agtctatcca tcccaaagtt gagatgattg aggatacttg aaaaaagata
     5041 ttcctttcta ccattatccc aaaaactgat tgtataaaga taaaggctca gtgcctcctg
     5101 accgataatc gggaggtagc actgtaccag agatgaggta tcttgcgaca cccgattatt
     5161 ctttagataa gaaaaacggt caattggctt catttatctt tcctttttct ttttagagga
     5221 ctgggtgatt tgttggagca agctctctaa ctcactgaca tccttaaaac tacgatagac
     5281 actagcaaaa cgtacatagg taatctcgtc caattcagcc aactcctcca tgacgagtga
     5341 accaatgtcc tcactttgaa tttcattttc atttcgacca cggagtttct gttcgatacg
     5401 attgactacc atgttgattt catcacttga cacaggacgt ttctgggctg agcggataat
     5461 cccattaaag attttatctc tggagaattg ttcccgtgtg ccatcttttt taacaaccac
     5521 taaggttctt tcttctactc gttcgtaggt tgtaaaacgg tgttggcact cgtcgcactc
     5581 tcgtcttcta cgaatggtgt tcccttcttc tgcttggcga ctatcgataa cacttgactt
     5641 ggtagcccca cattttggac aacgcatcct ttccctcctt atcgttttct tttcattata
     5701 ccatttttta agcgattccc aaaacaattc ttctttttgc ttgacaagtt ttttgttttg
     5761 ttgtattatt taattaagac aaaagggtaa gagaaaggag actaagatgt cctggacatt
     5821 tgacaacaaa aaacccatct atttacagat tatggagaaa atcaagcttc agattgtttc
     5881 ccatacactg gaacccaatc aacaacttcc aaccgtgagg gagctagcta gtgaggctgg
     5941 tgtcaatccc aataccatcc aaagagcctt atcagacctt gaacgagaag gatttgtcta
     6001 cagcaagcga acaactggac gatttgtgac taaggataag gagctaatcg ctcagtcacg
     6061 caaacaatta tcagaagaag aattggaaca cttcgtttcc tccatgaccc attttggcta
     6121 tgaaaaagaa gaactaccag gcgtagtcag tgattatatt aaaggagttt aagcctatgt
     6181 cattactagc atttgaaaat gtatccaaat catatggagc aacaccagcc cttgaaaatg
     6241 tttctcttga cattccagct ggaaaaattg tcggccttct tgggccaaac ggctcaggaa
     6301 aaacaaccct gattaaacta attaatggcc tcttacaacc agatcaagga cgtgtcctca
     6361 tcaacgacat ggacccaagc ccagcaacca aggccgttgt agcttatttg cctgatacga
     6421 cctatctcaa tgagcaaatg aaggtcaaag aagccctaac ctacttcaag accttctata
     6481 aagatttcaa tcttgaacgc gcccatcatc tacttgcaga cctgggcatt gatgaaaata
     6541 gtcgtctcaa gaaactatca aaaggaaaca aagaaaaggt tcaactgatt ttggttatga
     6601 gccgtgatgc tcgtctctat gttttggatg aacccattgg tggggtggat ccagcagccc
     6661 gtgcttatat cctcaatacc attatcaaca actactcacc aacttctacc gttttgattt
     6721 ctacccactt gatttctgat atcgagccaa tcttggatga aattgtcttc ctaaaagacg
     6781 gaaaagtcgt ccgtcaagga aatgtagatg atattcgcta cgagtcaggt gaatccattg
     6841 accaactctt ccgtcaggaa tttaaggcct aagcaaagga gattatttat gttttggaat
     6901 ttagttcact acgaatttaa aaatgttaac aagtggtatt tagccctcta cgcagccgtg
     6961 ctagtccttt ctgccctcat cggaatacag acacaaggct ttaaaaatct accttaccaa
     7021 gaaagtcagg ctactatgct actttttcta gctacagtct ttggtggctt gatgcttaca
     7081 cttgcgattt caaccatttt cttgattatt aaacgcttca aaggtagtgt ctacgaccga
     7141 caaggctatc tgactttgac cttgccagtt tctgaacacc atatcatcac agccaaacta
     7201 atcggtgcct ttatctggtc attgattagc accgctgtat tggctctaag tgctgttatt
     7261 attctggctt taacagctcc agaatggatt cctctttctt atgtgattac atttgtagaa
     7321 acacatctcc ctcagatctt tcttacaggt atatccttcc tactaaatac catttcagga
     7381 atcctctgca tctacctggc tatttccatt ggacagcttt tcaatgaata ccgtacagca
     7441 ctcgctgttg cagcctacat tggtatccaa atcgtcattg gatttattga acttttcttc
     7501 aatcttagtt ccaatttcta tgtcaattca ctggtaggac tcaatgacca tttctatatg
     7561 ggagcaggta tagccattgt taaagaactc atattcatag ctatctttta tctcggaacc
     7621 tactacatct tgagaaataa ggttaatttg ctttaaataa tttttaccta gatatgtaac
     7681 atactcatag aacaaaagag accaggcaaa aagtctttaa aattagaaaa cgcatagtat
     7741 caagtgttaa aaatccttga tattatgcgt tttttgtgag ctgacttatt tcctttcgct
     7801 atatcgcaaa atgaaataag aacggaacga tgggattttg gaattcaaat caatttataa
     7861 gaatgtttta gaagtaatat tatcctattc cagattcagt tcactataca attgagtttt
     7921 caagcaacct gtttacataa tgtgtacata attaggttcg tgattccacc cttttcacct
     7981 ttaaaaacct cgctttcgca aggctcttct atttataaga taaggcacgt ttaaaggttt
     8041 tccaaatccc taaatcatcc gtttgaagaa cgagactagc atacatgcgt ccgataaatc
     8101 ctgttgctac caccgcaaaa atcactgtaa tagcaagtga aatccatgct tctgctcccc
     8161 ccgcatagcc attaatcgtt cgaaacggca taaagaaggt cgaaataaag ggaatataag
     8221 aaccaatctt caagaggaga ttgtcaccag ctgcacctag agctgtcact ccaaaaaaac
     8281 cacccataat caaaatcatc aaaggcgaca aggctttccc tgagtcctca ggacgagaaa
     8341 ccatagatcc taggaaggct gccaagacta cgtacatgaa aagactgatc aaaataaaga
     8401 gcaaggtatt cagtgagata gcatctccca agtgatccaa aataccagac tgagccaaga
     8461 atggcaaatc tttaaagagc aaaacggcag ccagaccacc tacaacatag atcccaatat
     8521 gcgttaaaat cactagaaac agagccatca tccgcgcata gaaatagtga cttgccctta
     8581 tgctagaaaa aacgacttcc ataattttgg tgcctttttc actggcaact tcctgagctg
     8641 ttacacccgc ataggtaatc agaatcatat aaagaaagaa tcctaaggca cctgctgcaa
     8701 ttgtttgaat aaacttttta ttttccttgg cttcatcaat cttttctgtg aattgaattg
     8761 tctgcgctaa gcgtttttcc tgctcttgag acaaggaagc agttgaacga ttaagctgat
     8821 tttgcagttc attgagtgta cctgtaacct caaatttaat tccattttca agcgatgttt
     8881 cgccatgata aactgccttt agaacactat cttcttgatc aatggtcaaa taacctttta
     8941 atttttcttc tttaattgct tctttggcac ttgcttcgtc tttatagtcg aagttaacac
     9001 catttacatt cttcagtcct tctgctacag atggcactgt tgtcactact gccactttat
     9061 tatttttagc catagaagaa ccttggatat gcccaattcc tacagagatt cctaaaaaga
     9121 ggaacggcga aatcaccata aagaagaaac tccatgactc gacatgtcga agataggttt
     9181 ccttgattac aacccacata tttctcatac ttccactcct gattctagtt taaagatttc
     9241 atcgatagtt ggcgcttgtt ggtcaaatgt tgcgatatat tgaccttgag tcaagattgg
     9301 gaagagttcc cttccagcgc tctcatcctc caaaatcaat ttccaactgc cttgtttggt
     9361 caagctcacc tgtttgacat gaggaagatt ttccaattct tccttgcttc gttcacttga
     9421 aacaaagaga cgcgttttcc cgtattgatt gcggacatcc tgaactggtc cgtgcaagac
     9481 cacacggcca tctcggatca tcagaatatc gtcacaaagt tcctcaacat tggtcatgac
     9541 atggtcagaa aagataatgg ttgctccgcg ctctttttcc tgaaaaatga cttgtttgag
     9601 caattctgta ttaactgggt ccaatccact aaaaggctca tccaagataa tcaggtctgg
     9661 ttcatgaatc agagtaataa tgagctgaat cttctgctga tttccttttg acagactctt
     9721 gattttatct gtcagctttc ctttcacttc caacctcttc atccattgag ggagtttttc
     9781 tttgacttct ttggcatcca tgccttttag agtcgccaag tagcgaactt gttcaagaac
     9841 tgtcaattta ggcatgagac tgcgttcttc aggcagataa ccaatccgag cataggtctc
     9901 ctgacgaata tcctgaccat ccagaccgat ttctccctga tattctagga atttcaaaat
     9961 actatggaaa atcgttgttt ttccagcacc attttttccg actagtccca aaatacgacc
    10021 tggtcgcgct tgaaagtcaa taccaaacaa aacttgcttg gagccaaaac ttttctctag
    10081 acttcttact tctagcatct ttcacctccg aaatttcttg cactcattat actccttttt
    10141 gatagccttt acaatgtttt tgtccatttt tagaagacta ttgctgtgta aaatatggcc
    10201 tggagcactt ttatactcaa tgaaaagcaa agagcgaact aggaagctag ccgtaggctg
    10261 ctcaaagtac agctttgagg ttgcagataa aactgacgaa gtcagctcaa aacactgttt
    10321 tgaggttgtg gatagaactg acgaagtcag taactatatc tacggcaagg cgaagctaac
    10381 gtggtttgaa gagattttcg aagagtatta gtgataaatc cattatacag tagcaaactc
    10441 aatttatacc ttccgctcct caactgtcta tttttaatcc tgaattgtta tttgagtaac
    10501 tcctttttcc tcataaagtt ttcttcctct aaaacttctg gaaaaaagcg aatagtttca
    10561 gacaacattt ttataagaaa caagttcatc tgtcatttca agaaggagta atcctttatc
    10621 tactaatgga cggaacggaa ttcaaccgct tgtccgatat attttctaag gattatatag
    10681 taaaatgaaa taaaaacagg acaaatagat cgggacagtc aaatcgattt ctaacaatgt
    10741 tttagaagta gatgtatact attctagttt caatctgcta tatctattat gcacacccct
    10801 ataggatcta atgaaaatca caacaggctc attcatagat ggttacctca ggaaactaag
    10861 aaaacgacta caaaggaagt cgcattcatc gaaaagtgga ttaacaacta tcctaaaaaa
    10921 tgcttgaact acaagtcccc cagagaagac ttctggatga ctaacttgaa cttgaaattt
    10981 agcaataatt aattcaccat ctaactatat ttagtaatta tttcagaact gattaatatt
    11041 aaaattaact aacaattcaa aggattcata ctagccataa attacgtcca tcagagagag
    11101 actcttacta cttttatatt ttagtctttc tggcttcagg atacatataa actttaggga
    11161 aaatgactat tcgaaagcgc gaatgcctca aaattatctc agataagcta ttcgaaactt
    11221 agaatgcttt taaatttatg gaattgcgat tattcgaaac ctagaatgca tataaccttt
    11281 agttgacaga cctattctaa gtctcgaagg gctatttact ttctattcct tatcaaaaaa
    11341 gactcattcc ccctttctcc tccaaaatat ggtatagtag aaatatacta tctatgagga
    11401 gtttacatgt cacaggataa acaaatgaaa gctgtttctc cccttctgca gcgagttatc
    11461 aatatctcat cgattgtcgg tggggttggg agtttgattt tctgtatttg ggcttatcag
    11521 gctgggattt tacaatccaa ggaaaccctc tctgccttta tccagcaggc aggcatctgg
    11581 ggtccacctc tctttatctt tttacagatt ttacagactg tcgtccctat cattccaggg
    11641 gccttgacct cggtggctgg ggtctttatc tacgggcaca tcatcgggac tatctacaac
    11701 tatatcggca tcgtgattgg ctgtgccatt atcttttatc tggtgcgcct atacggagct
    11761 gcctttgtcc agtctgtcgt cagcaagcgc acctacgaca agtacatcgg ctggctagat
    11821 aagggcaatc gttttgaccg cttctttatt tttatgatga tttggcccat tagcccagct
    11881 gactttctct gtatgctggc tgccctgacc aagatgagtt tcaagcgcta catgactatc
    11941 atcattctga ccaaaccctt taccctcgtg gtttatacct acggtctgac ctatattatt
    12001 gactttttct ggcaaatgct ttgacacgta aaaaatccgt ttggtttccc aagtggattt
    12061 ttaaagcgta gattaactat agcttgatac taaatatact ttggtatgga aatcatgcat
    12121 atttttcgat agtgaggcga ggacttacct agccttcccg ccgtgataga aacacctgaa
    12181 atctaatggt ttcaggtatt cggaaacttt gagcctagtg tctcaaagtt taggtatgga
    12241 atttcgaaga aagtcgctac cgtccgtaat cacttaagga aaggctcaaa aatattgttt
    12301 tcaaccacaa aatccgtttg gtttcccaag cggattttgt gctttatttt gaaacttctt
    12361 ttgcaagaac aaagttccca agtgtggcag aaccatttcc tgcgactgct ggcgtcacga
    12421 tatagtcacg cacatctggt actggtaggt aaccattaag aagagatgta aatttctcac
    12481 ggacacggtc cagcatatgt tgttgagcca tgacccctcc accaaagaca atcacgtctg
    12541 ggcggaaagt cactgtcgca ttaaccgcag cttgagcgat atagtaggct tgaacatccc
    12601 aaacagggtt gttgagttca atagtttccc cacgtacacc tgtacgagct tccaaacttg
    12661 gaccagctgc ataaccttct agacatccct tatggaaagg acaaacaccc ttaaactctt
    12721 tttcaatatc cattgggtgt ctagcaacat aataatgacc catttcaggg tgacccacac
    12781 caccgataaa ctcaccacgt tggatgacgc ctgcaccgat acctgtaccg attgtgtagt
    12841 aaaccaagtt ttcgatacga ccaccagcat tgttacgggc aaccatttca ccgtaagcag
    12901 agctgtttac gtctgttgtg aagtacattg gcacgtttag ggcgcgacga agggcaccaa
    12961 gcaagtccac atttgcccag tttggttttg gagtcgtcgt gataaagcca taagtttttg
    13021 agtttttgtc aatatcaatc ggcccaaatg aaccaactgc aagaccagca aggttatcga
    13081 attttgagaa gaactcaatg gttttatcga ttgtttcgat tggagttgtt gttggaaatt
    13141 gtgttttttc tacaacgtta aagttttcat caccgacagc acagacaaac tttgtaccgc
    13201 ccgcttccaa gcttccatat aattttgtca tgataaacct cttgttttta ttttctttat
    13261 tatagcatac ttcgaaagtc taaatgtctc tattttttag attttcctct gtaaatctta
    13321 ctatctaata aaaacgaaca aacatgtcat ttgttcgttt tcacattaga gaggattgat
    13381 tagattttca cttcgatcac agcatccccc ttcgcaactg aacctgttgc gactggagct
    13441 actgaagcgt agtcagctgt atttgtaacg ataaccattg ttgtatcatc aagtccagct
    13501 gcagcgattt tgtttgagtc aaatgttcca agaacatcgc caactttcac cttatcacct
    13561 tgagcaactt ttgcttcaaa accgtcaccg ttcatagata cagtgtcaat accaacgtga
    13621 atcaaaactt cagcaccatt tcttgttttc aaaccaaaag cgtgccctgt tggaaaggca
    13681 attgaaactt cagcatcagc tggtgcatag accacgcctt ggcttggttt cacagcgata
    13741 ccttgtccca tagctccact tgagaagact gggtcattga catcagcaag agcgacaaca
    13801 tcaccaacga taggagttac aagtgtttca ttttgaagag tatcagtctc ttccactact
    13861 ggtgcttcta ctgtaggttc tgtaactgtt tctgcggttg ctgtggcagc ttcatcagca
    13921 aagacactag ctgcttttgt tttcccgtag aaataagtga aagcaaaggc ggcaacaaag
    13981 gacataattt ccgcaattat atacataggg attgaaccag ccttaataga aaggaatcca
    14041 ataaatccag ccgctcccaa tgaaactgca ataacgtgag ttaaacctgc tacaaaggca
    14101 ccaattgctg aaccagcaag ggcgcagaag aatgggaatt tgtatttcaa gtttacccca
    14161 aagagagcag gttctgtaat accaaggaag gcagaaacag cagccgaact ggacaatcct
    14221 tttgttttag cattttttgt caagaagtag actgcaagag tagccgcacc ttgtgctacg
    14281 tttgccattg aagcaacaac aaagatgtag tctccaaatc cagtaccgtt attattgtat
    14341 gcagacaaca attgtgtttc gattgctggg aatgattggt gaagacccgt cataacgatt
    14401 aatgagtaag ttccaccaaa tagccccata ccaaaggcac ctgttgtgtt gtacaaccat
    14461 acaatcgcat ttgtaatcgc atcagacaca accaacataa caggaccgat aactgtaaag
    14521 gtaaggaatc ctgtaatcat cactgataaa agcggtgtaa aagtgaagtc aactgcagat
    14581 ggcaatttct tgtggaagaa tttttctaaa ttagctaaca accagactgc aacaaggact
    14641 ggaataacct gataagcata gtttgtttgt gtaacatgga aacctaagat gtcccagaag
    14701 gcttctttac cacccaaatc tggagtggtc ataatcatac cgattgccgc accaaggaat
    14761 tggtttgctc caaagcgctt ggctgctgaa ataccaacca aaacagggag gaacataaat
    14821 ggagcagcag acatcaattg aatcatagct gagaagcctt tgatagcagg atacatatct
    14881 tcaattgatt gaggtccaaa caaatctggt gatgtcaaga agttccgtaa agccatcaag
    14941 agaccacctg caactaaggc agggataata ggaacaaaaa tgtctgacaa aagtttaatc
    15001 aagtccatca atggattgaa ctttttgtct ttattagcaa tctctttcaa atcatctgtc
    15061 gaaacttctt tcaaaccagt tttcttaata atttccgcat atacgaagtt aacatcacca
    15121 ggaccaatga taatttgata ttgtccgtta gtgctaaaag tccccttgac atccgcatta
    15181 ttatccagag ctgcttgatt cacttttgct tcgtctttca ataccaagcg aagacgtgtc
    15241 gcacagtgag ctgcagctac aagattatcc ttaccgacag ccccaatgac ctcggcagca
    15301 actttactat aatccatagc aaaaatctcc ttatataaca ttttgttctt gtttgaaagc
    15361 gattttattc gcccttacga ctattatttt atcatgtttt aaaaatatgt caagcgtttt
    15421 gcagaaaaaa tattctatcc acttagtggg caagttttag attgattgtt tttgactgtt
    15481 tcatcaggaa ttccttgaaa aataagtttg aaaccttggt taaaaagtct tttatttcat
    15541 tatctttcac attttcgtaa aaatttgatt gatttcctgt atttatttta tgataatcgt
    15601 ttgacagttt ttcctctttt ttaacataaa aggcttgcat tttttatcga aaacagttac
    15661 tattaaaagt gataagattt ttggaggaat gaatggaatg gacaactgag cgtcgttaca
    15721 gactttatca agattggacg caagaagaaa ttcaacatat aaaggaaaat atggcacaat
    15781 ctccatggca tactcattac catgttgagc caaaaacagg acttctcaac gacccaaatg
    15841 gcttttctta ctttgatggc aagtggatcc tcttttacca gaattttcct tttggtgcag
    15901 cccacggttt gaaatcttgg gcacagctag aaagtgatga tttggttcac tttaaagaaa
    15961 ctggaatcaa agttttacca gatactccat tagatagcca cggtgcctac tctggttctg
    16021 ccatgcaatt tggcgataac ttattcctat tttatacagg aaatgttcgc gatgaaaact
    16081 ggatccgtca cccataccag atcggtgctt tgatggacaa ggagggtaag attacaaaga
    16141 ttgacaagat cttgattgac cagccagcag actctactga ccacttccgc gatccacaaa
    16201 tttttaactt taaaggtcaa tactatgctg tcgtcggtgg acaggacttg gagaaaaaag
    16261 gcttcgtccg tctctacaag gctgtcaata acgactacac aaactggcaa gcagttggcg
    16321 accttgactt tgctaacgac cgtactgcct acatgatgga atgtcctaat ctggtctttg
    16381 tagaggaaca tcctgtcctt ctctactgtc cacaaggatt ggataagaaa gttctagact
    16441 acgataatat ctttccaaat atgtataaga ttggggcttc ctttgaccct aaaaatgcca
    16501 aaatggtaga tgtgtctcaa cttcaaaaca tggattacgg tttcgaagcc tatgcaactc
    16561 aagccttcaa cgctcctgat gggcgtgctc tagcagttag ctggcttggt ttgccagatg
    16621 tttcttaccc atctgaccgt tttgaccacc aaggaacctt ctctttggtc aaggaactca
    16681 ctatcaaaga cgacaagctc taccagtatc cagtcgctgc tattaaggac cttcgtgctt
    16741 ctgaagaagc cttctcaaac cgttcccaaa ccaagaacac ttacgaactt gaactcaact
    16801 tggaagctaa tagccagagc gagattgtct tacttgctga taaagaaggt aagggacttt
    16861 caatcaactt tgaccttgta aacggtcaag tgacagtgga tcgtagccag gctggagaac
    16921 agtatgccca agaatttggg acaactcgtt cttgccctat cgagaatcag gctactactg
    16981 ctacaatctt catcgataac tctgtctttg aaattttcat caataaagga gaaaaagtat
    17041 tttctggtcg tgtcttccca catgcggacc aaaatggtat cctgatcaaa tctggaaacc
    17101 caactggaac ttactatgaa ttagattatg gtcgcaaaac taactgatgt cgccaaactt
    17161 gcaggcgtca gtcctactac cgtttctcgg gttatcaata aaaaaggata tctatctgag
    17221 aaaaccatcc aaaaagtcaa tgaagccatg agagaattag gctataaacc caacaacctg
    17281 gctcgtagtc tgcaaggaaa atcagctaag ttaatcggct tgattttccc caatatttcc
    17341 aatgttttct atgcagaatt gattgataaa ttggaacacc aactcttcaa aaatggttac
    17401 aagaccatca tctgcaacag tgaacatgat tctgagaagg aacgcgaata catcgaaatg
    17461 ttggaagcca atcaggtaga cggcatcatt tctggtagtc acaacctagg aatcgaagac
    17521 tacaatcgtg tgacagcgcc gattatttcc tttgaccgaa acctatcgcc agacatccct
    17581 gttgtctcct ctgacaacta tgctggtggg gttcttgctg cccaaacctt ggtcaagaca
    17641 ggtgcccagt ctatcatcat gattacaggg aatgacaatt ccaattcgcc aaccggactg
    17701 cgccacgctg gttttgcatc cgtactccca aaagctccta ttatcaatgt ttccagtgac
    17761 ttttctcccg tcagaaaaga aatggaaatc aagaatatct tgacccggga aaaaccagat
    17821 gccatttttg cttcggatga tttgacagct attctggtca ttaaaatcgc tcaagaattg
    17881 ggcatttctg tcccaaaaga gctcaaggtc atcggctatg atgggaccta ctttatcgaa
    17941 aattactacc ctcaattggc tactatcaag caacctttgg aagagattgc ttgtctcact
    18001 attgatcttc tcttgcaaaa gattgaaggc aaggaagtcg ccacaactgg ttacttctta
    18061 ccagttacgc tattaccagg aaaaagtatt taaacacaag aaaactcaga ccgattcgtc
    18121 tgagttttta tgatcttaaa ttttcgagat agcgctgggc tgtctctagg ttaaaggttt
    18181 tatctgagat gaggcgctct actaggggag caacttcaga ttcactagcc ccagctagga
    18241 gagctaggga tttggcctgt agtttcatgt ggccttgctg gatgcccgta cttaccaagg
    18301 ctttgagggc tgcaaaattt tgagcaagac cgatggacac gataatctgg gctaattctc
    18361 tggcagaagg atttcctagt agatcatgac tgagagctac acgtgggttg aggccgatag
    18421 agccaccctt agtcgctaca ggcatgggca gggtcatctc accgaccaat tcttctcttt
    18481 caaggtccag cgtccagcag ctaagacctt gatagcgtcc atctcgactg gcaaaggcat
    18541 gggccccagc ttcgatggca cgccagtcat taccagtggc aatcaaaatc gcatcaatac
    18601 cattaaaaat tcctttatta tgagtagcag ctcggtaagg atcagcctgc gcaaactgac
    18661 tagccaacgc aattttctcc gcaatctctc gtccttgatc cttttggcgg ctcaagtagc
    18721 gaaaggcgat gcgacagctt gcagtcacca gagaatcagt cgcgtagttg gacaggattc
    18781 ccatgagact ctgtccctga ctgagttctt ctaagactgg tttcaaggct tccagcatgg
    18841 tgttgagcat attggcaccc atggcttcct gggtatcgac atgaatataa acaacgagaa
    18901 agtctggttc gccttttatc tgctcgacat gcagatcacg cgccccacct ccacgtttaa
    18961 cgatagaagg ataggcttga ttggcaagct ccaagagctc cgctttcttg ctggcaatct
    19021 tctcttgcgc tagtttagga ttagcaactt gataaagggc tacctgccca atcatctggc
    19081 gctgatggac ttgtgcagta aaaccacctg cacgcttgat gattttgctg gcatagctgg
    19141 ccgccgcaac cacagagggt tcttctgtca catagggaac ggtgtattcc tgaccgttga
    19201 caagtacctc cggaaccagc gaataaggca gagaaaaagt tcccaccaca ttctcactca
    19261 gctggtctgc cacagtcaca ctcatctgtt catccttctc cagactagct tgtctctcag
    19321 gactaaggag cgcctgagct tttagcagct cgaggcgctc ttggtatgat tttttagaaa
    19381 atccattcca acttatcttc attatttttc aaccttgcta taacggcgtt ggtggtcaag
    19441 aatttcaacc aaggcaaaat cttgattttc atagccagca aactgggcag attttgtttc
    19501 atccaagttt acttcctcaa aaaagacctt ttcatagtct gcaacggata gggcagttcg
    19561 ttggttgagc ttgttcaagc ggtctttatc caaataagct tcatatcctt caaccaattc
    19621 accactgaag aactcagcca cagctccact tccgtaacta taaagggcga ttttatcccc
    19681 agctttcaag ctatctgtat tttccaagag agacaaaagt ccaaggaaaa gtgaacctgt
    19741 gtagatattc cccacctttt gactgtagag aatagactgg tcaaaatgct tttgtaagag
    19801 gtcttttttc tcttgaggca ggctcttatc catgattttt ttcaagcctt ttagcgctaa
    19861 tttaggataa ggcaagtgga aacaaacagc cgcaaaatca tccagagtaa gctggtagcg
    19921 tttttgatat tcaagccaag tcgttttcaa actatccaag tattgttggg tagaataaac
    19981 accatttaca taaggagttg tcgagtaatt tggtcgccag aaatccatga tgtcacgggt
    20041 ctgagctaca ttgtcattat taaaggccat catgcgtgga ttttgtgtaa tcaacatagc
    20101 tacacttcca gcaccttgag ttggttctcc cggagtttca ataccatatt tggcaatatc
    20161 actggcaatg accaatacct tggactccgg agaattttcc acatgcaatt tggcataatg
    20221 gagggcagct gtcgccccat agcaggcttc tttaatctcg aaactacgag caaagggctg
    20281 gatgcccagc aagccatgca caaagacggc cgcagcctta ctctggtcaa ttcctgactc
    20341 ggtagccaca atgaccatgt caacttcttg tctttcttgc tcagttaaaa tagagtcact
    20401 agcactggcc gccaaggtca cgatgtcctc agttaggggc gcaatactca attccttgag
    20461 taagagtcct ttacttaatt tttcagggtc aattcccctc gcttctgcta agtcttgtaa
    20521 tttcaagaca tattgactgg tcgcaaaacc aatcttatca ataccgattg tcatatttac
    20581 ctctgtttta tcattcatgt aaaaaatcgt tctatactat tttatcacaa atggcagtaa
    20641 aagagagaaa aaagacttga ttcaccaaat caagcctctt attggtcatc attttaaaga
    20701 atgattagtt gctagagagt tcatcgatat aagtagcttt ataagctcca ttcacagtta
    20761 tcagctcctg gaggatcaaa tttcctgagt aagtccttcc catctcatct acaaattttt
    20821 gataaaactg actggtcgga atttctctga catccttatc aaatgtcttg tcaagtgttt
    20881 tactaacctt ctcagcaatc aattgatgct cttgccatcc actttgaaac tctgagcccg
    20941 aactagaaac catgactggg ataaacaaca aggtcagtag acttacagac aataaggaaa
    21001 atagtagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc aacaatcaaa
    21061 ttcattcgta atccgaagcg tttacgatga tttcgatagg ttgttgaaat cattttaaac
    21121 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttagatag cgcatggtta
    21181 caggctttat cttcagctgt tagcggcttg agtttgctgg atttacgtgg agtttgtgct
    21241 tgaggatata tcttcatgag cccttgataa ccactgtcaa ccaagatttt accagcttgt
    21301 ccgatatttc tgcgactcat tttgaacaac ttcatatcat gacaatagtt cacagcgata
    21361 tccaaagaaa caattctccc ttgacttgtg acaatcgctt gaggttccat agaatgacat
    21421 ttctatttta ccagaataat tggctagttg atttttttag gacgatttat ttttacctct
    21481 gttgcatcca caatcaccgt attctcagca ctaagatgag tttttgaaat cgtaacacca
    21541 ctttgaacaa gagttacttc aacccattgg ctccgacgga ttaagttgct ttcgtggata
    21601 ccaaaatcag ccgtaatttc ttcataagtg cggtattctc gcacatatag aaagcgttgt
    21661 taatttattt atctcatttt tcagaaaatt cttttatttc tgtaaagtct acgatactcg
    21721 atgtgttttt atataatgat acagtctgag aatcactgtt ccgctagcca ttccaataga
    21781 gattaccaga gccaacatga caaccaaggt cgcacttgcc agtgctttat tatagtcccc
    21841 tgtcacaaaa aaggcagttg ttcggtagga gagataacct ggaaccagcg gtgccaaaat
    21901 ggccaagata aagaccacag caggtgtctt ataaagaata cttaaaatct ggctgacaca
    21961 agaaccaata atggctgcaa tgaaggtagc tacaatgaca ttggtcggtt ccttgagcaa
    22021 gagatagatt agccagacag tcatgcccaa aatccctcca ggtaagagca tagaccgttg
    22081 cacattgagt acgattaaaa aagtgataat ggcaagaaaa cttgctactg cttgtaataa
    22141 aaaggttgtt agtgtcatat tagttcatca ataccaaggc gacagaagtt cctgccccta
    22201 aagcgagggt aatgagcagg gattcaaaca tcttactcat accagagttt atgtggttgg
    22261 tcataatatc acggaccgca ttggtcaagg caatgcctgg tacaaacggc atgaccgcac
    22321 cagctataat caaatctgcc gttgaaggaa aacctgtgta gcgagcccaa aactgggcaa
    22381 ttatcccaaa gacaaaggct ccagcaaagg ctgtcacaaa gggaattcgg ataaattttt
    22441 ccacatagag ggaaaaggca aaaccaaata aggtcgccac tcctgcccca agtgcgtcgt
    22501 agatatttcc gctaaacata actgaaaaga aaggagcact aaaggtcgca gccagagtta
    22561 cctgcaactt agtataggga aggggttgag cttgcaaggc cgtcaattgc ttaaaggctg
    22621 tttctaagtc aatctgcccc ccaactagct gacgagaaat ctggttcaca tcgcagactt
    22681 tttcgatgtt ataagaagag gaggtcacgc gcttcatgcg cgaaatattg gtattttcaa
    22741 tagagaaaaa gatagcggca ggcatggcaa ggacattgca atccacaatc ccctgcgaat
    22801 gcgcgattcg aatcatggta tcttctacac gatggatttc tgagccactt ttaaggagaa
    22861 tagtccccgc tagcataatc acatcaatga cggcatttaa ttcttttgat tcttccatgc
    22921 tttcctcctt ttatcaactc cctctattct atcacaaatc tggactcaaa aaaaatcttt
    22981 gccatgaaat catgacaaag attgattact cattttgatt atccatctgc ttttaaggag
    23041 tagctgaagt tgttttaggt ttgtagattg aaatcttgac tctagtctta tttaggtcta
    23101 ccttttcacc tgctctagga ctttgttcaa caaccatgcc ttctacacta cctgcaggcg
    23161 ctgtcgtcac ttctacaact tctatattag cttccttaat cccaacaatt tgaatcaaat
    23221 tgttcttagt aaactccaag ctggaaccaa tgtaactcgg catggcaaca cttgtaactt
    23281 ttttagctac tgtcaagaca atttgagtag gtttactcac atcataagtc gttccggcac
    23341 ctggactttg tttcataatc gttcctggtt cgctttcgct ggactcttct tcctctatct
    23401 taatcaaatt ctcaggaacc ttcttctgct tgagttctga gattacttct gtagagttcc
    23461 gtccaatata gttccctaat tgaatcgtcg tagctttttt agctactgtc aaaacaattt
    23521 gagttgcctt gctcaagtca taggtcgtac cttctggtag actttgcttc aagaccgttc
    23581 cagcctcact ctcattcgac tcttcttcct caattttaat caaattatct ggaacttttt
    23641 tctcttttaa ttccgcgata acatcagaag atttccggcc gacataatta ctaatttgga
    23701 aggattgttt gcctgatgag acaaccagat tgattttcgt tccttctttt cgaccagttc
    23761 cagcgccagg atctgtacgg ataatccgcc cttcttccac cttttcacta gcctctgtct
    23821 tctcctcacc aatctcaaaa ttggcttttt tgagcgttgc cttggcctct gcaactgtct
    23881 gacctgccac atctggaatg gcaatggttg caggagttct ggatagtatc caaataagag
    23941 aagctgccac caatacaagg ctggccaaca aaatcaggta acgcatctta aatctatgtt
    24001 ttttcggtgc ttgtggttgg taagtttcct ctgtcacagc ctggcttggg tttttgattg
    24061 atttgtgttc tgtctgcgct tgagccttag gaatagatgt caaggtactc tgagaaacct
    24121 tcggcaaggt cttggtatct gccttgctcg tttcatcaaa gattaactta ctttcatttc
    24181 tacgattgta ggacaagcta ctagacaagt ccacatacat ctctgaaacc gagcggtagc
    24241 gattggtcaa ctttttagca gtcgccttga taataacatt ttctaaagcc tgaggtacag
    24301 atggattttc tgcaataacg gacggcaggg gtttctggaa atgctggagg gcaatggtca
    24361 ccgcgctatc cccgtcataa gggatatggc ctgtcaacat ctcatagaaa ataatcccca
    24421 tggcatagat atcactctgc acagtcgcct tcgaaccacg cgcctgctct ggtgacaagt
    24481 aatgaactga gcccaacatc gagttagtct gggtcagact tgtctctgca aaggctacag
    24541 caatcccaaa gtctgtgacc ttggcagtcc catctggtgt caaaaggata ttttgaggtt
    24601 tcaagtccct gtgaacaatt cctcgagtat gggccaagcg catagccaag agaatttgtc
    24661 ccatgatacg gactgcttct tcattagaaa gaggataatg ttccttgata tagcgtttga
    24721 ggtctagtcc agcaacatac tccattgcaa gatactgttg accgtcttcc tcaccaatat
    24781 ctgttatccg aacgatatga ggatggtcta gatctgccat agctctcgct tcacgctgaa
    24841 aacgagctac agctatcggg tccgtctggt agttggtcct cagaaccttc actgccactt
    24901 cttccccatc taagattaag tctttggcta ggtagacatc cgccatgcct cctcgaccaa
    24961 tctgtttgac aatccgatag cgtccggcaa aaatcttgcc gatttggatc attctgcatc
    25021 ctcctcgttc atagaaacaa gggcaaccgt aatgttgtct aaacctcctg cattgttagc
    25081 aaaacgaaca agtgtctccg ttttatctgc taaaggaata tcactggtta caatatcacg
    25141 aatctcactg cctgaaatca tgttggtcaa gccgtcacta ttgagcaaga gatagtcacc
    25201 tgactcaagg ataactgtcc caaaatcagg ctgaatttca tctttttgcc caatagactg
    25261 ggtgataata tttttttgcg gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt
    25321 gagcaattca ttaaccaagg aatgatcgct cgtcaactga tggtattctt ctccacgaat
    25381 caagccgata cgcgaatcac caatatgagc atagatagcc tgattatcaa caatagcaag
    25441 gacttccaaa gtagttccca tgcctctgta agcttcatcc tgaccaagct ggtgaatctt
    25501 ttgattttca atttctaggt aatgggcgaa ccattcacgc acttcattga ctgtatcgat
    25561 ctgggtatca acccaagcta cacccaggtc tgtgaccgcc atttcactag cgatattccc
    25621 tgcacgatga cctcccatcc catcagctaa aataatcatg gtacgtccag ctctattgac
    25681 atagtggttg acatagtctt ggttatttgt tcgtttctga ccaacatctg ttaataatga
    25741 aatttccatg tgtcagttcc ttcctaatcc gatatcttgc gaaattgact gatgaagaat
    25801 ccatcacttc catacaattc aggtgtaatg aggatacagc cgtctttcat gatatcctta
    25861 cattcatgtt ctagttttac ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg
    25921 acttgaaaat tctcctctga gacgatagtg caggtgctat aagttattat accacctttg
    25981 cctagtattt gacaaacact acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa
    26041 tctgccgttt ctttattgta tttgatatct ggttttcggc gcaaaagacc gattcctgaa
    26101 caaggagcat ccaccaaaat cttatcaaag gaatcctggt caaaaaactc atgcaccttt
    26161 ctggcatcca atttttgagt ttgaacccga tctgcaactc ccagacgttg ggcattttct
    26221 tgaattaaat ccaacttgtg gtcgtacaag tccagagcag taacctgacc tgtcgtaaga
    26281 taagaggcta tatgggctgt tttcccacct ggagccgcac aggcatcaag cactcgctca
    26341 tcaccttgta aatcaagcgt cggagcaacc aactgactgg actcgtcttg gatggtaatg
    26401 gctccatccg caaacaaatt atgccctgca aaatgcccct gctccttaac cagaccagtg
    26461 gttgctaaaa gggaattatt cgcctccaac aaggcttgga tttcctcttt tcgacttagg
    26521 tctgttacac gaatactggc tttgtttcgc actaacaggc tttcaaagat ggcttttgct
    26581 ctctcctctc cgtattcttc cttgagtttg gcaactagcc aaactgggag agaataggca
    26641 atggagtcac gcttgttttt tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag
    26701 atacggcgaa ggacagcgtt gaccaatttt tcactgcctt ttttacggag tttggccaat
    26761 tccactgctt cattgaccac agcatgatct ggaatcttgt ccaaatagcg gagttggtag
    26821 gcactcatga gaagaaggac atagagccag ctgtctaact ggtctctgtc ttcgataaag
    26881 tgggataggt accattccag agtcagttta cgggctaccg ttccatagac cagctcggtc
    26941 actaagccct tgtctgctgc caaaagttga cttcccttta gatgcttatt taaggcgata
    27001 tttgagtatg cttggttcac aaaaacatcc tctagcactg ctagagctaa acttctagcc
    27061 gtttctactt tagtcaccaa atcgttctcc tacagtcaat gtacgtccaa ctccgttgag
    27121 gaaggaagca atgtccatct taggcttacc agctggctgc acttgtttga gggatagagc
    27181 cccttcagcc gttgcgacaa tcaattcttt cttgccgata gagagaatct cacctggatt
    27241 tccctgacct tctactggta gggcttcata aatcttaaag cggtcgccct taaggaaagt
    27301 atgggcaaca ggccaggggt tcattccacg aatttggtta aagagttgac gattggtttt
    27361 gttccagtcc agtttttctt cctctggctt tatatttgga gagaaggtaa cctgactcgt
    27421 atcctgcggt tcaggtttga tatcaccagc aatataggca ggcagagtgt ccaaaagcaa
    27481 atcacgacca actagcgcca atttttcaaa caaggtgcca acattgtcct catctgtgat
    27541 cggaatgctg cgacgagaaa tcatatctcc tgcatccatt tccttaacca tttccatgat
    27601 ggtcacacca gcttcctcat ccccttgaat caaggcataa tggataggcg caccaccacg
    27661 gtgtctagga aggagggagg catgaacgtt gacagcaaag tccatgctat caaggagttt
    27721 gcttgggaga aactgcccaa aagcagcagt cacaattcca tctgctccta gcttcataag
    27781 atcttccatc tctggacttc cagataattt ttcaggttgg tagatagata gtcctgcttc
    27841 cttggcagcc tgcttgactg gggtttcttg gataactttt ttacgaccaa cagcacggtc
    27901 tggctgggtc acaacggcta gaatttcgta acggtcatct gtcaaaagtc cttttaagac
    27961 tgttgctgaa aagtcggggg tccccataaa gattagtttt gtcatatctt ctccttctta
    28021 taaaaattgc tgcggctcat ggtcaatact gagacggagc tcactatttt cccgttcttg
    28081 agtcaaggcc aggacctggt tgagggtcgg ccccagctca tcttctaaac ggtatttaat
    28141 taaaatctgg taatgataga ggttgtgggt acgggcaatc ggttttggcg ttggccccag
    28201 aatgggactg gtctctgaca agcctgaccg caaaatgttc atgacttcat aggcacgttt
    28261 gaaaacctct tcttctttct tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg
    28321 atagccgagt tgtcgtctga ttcccatttc ataggcataa aagccttcgt aatcttgatc
    28381 cttggcaaat cgaatagcat agtgctgcgg attgtaggac tgtatcaaga cttgacctgc
    28441 tttttcagcc cgacctgctc gacctgccac ctgagtcaag agctggaagg ttctctcaga
    28501 agaacggaaa tcaggcagat tcaaggccgt atccgcattt agaactccga ctagggtaac
    28561 attgggaaaa tccaaaccct ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc
    28621 tcgcccaaac tggtcaagca aggcttggtg actgcctttc tttcgagtcg tatccacatc
    28681 catcctcaaa atgcgagctt ggggaaagag ttctgctagc tcatcataag ccttctgagt
    28741 tcccgtccca tagtaacgaa tactgcggct cttacagtta ggacagacct gaggaatatc
    28801 cttcgagaaa ccacaataat ggcagttcat agtcttggta tccatatgca aggtcagaga
    28861 aatatcgcag ttgggacaag tatccaccgt cccacactcc cgacacatga caaagctaga
    28921 ataaccacgg cgattgagca tgagaaccac ctgctctttt ttaaccagac ggtcttggat
    28981 agcctctagc aaaggaggcg taaagtttga cgtctcattt tgtccgatat agtctcgaaa
    29041 gtcaatcact tgaacctcag ggattgtagc caaaggattg gcacgttggg ttagacgtaa
    29101 gtgttgatag acgcctttgc cagcacgtgc ccggctctct aagctcggcg ttgcagatcc
    29161 aagtaccaga gttgcttgat tatactgagc ccgtaaaata gctacctctc tggcatggta
    29221 acggggattg ctgtcctgct tataagccgc ttcatgctct tcatcaataa tcatgacacc
    29281 cagatttttc agaggagcaa agatagcaga tctggcacca acaacaactt gggcatcgcc
    29341 acgctccacc ttgcgccatt catcatactt ttcgccattg gataatcctg agtgaagaat
    29401 ggctaccttg tccccaaaac gtgctataaa acgctcggtc atctgaggag tcaaggaaat
    29461 ctcaggtacc agcaaaatag ctgtcttacc cttatccagg gcaccttgga taatctgcaa
    29521 gtaaacctcg gtcttcccac ttcctgtaat cccttgaagt agaaagggag gttgagaact
    29581 gccaatagaa ctcacaaccg catcacgcgc ctgtctttgt tctggattta actccaaagg
    29641 tctacttgct tcaattcctt caaaataagc agccgagcgt tgaacttcct tttggactat
    29701 ggtaacagca ccttgatcca caaagaagtt gacttgctct cgcgagtagg actctaacaa
    29761 gctagccaag gaagcgctct ctggatgaga cagcagataa tctctcagtt ccaacttttt
    29821 cttggcacgt gtagaaatct caacaccttc taattgggca tggtcaacct cataccaaga
    29881 ctgggtcttg accttctttt gatcgactgc ctgatattcc agaccaagca ggccttttct
    29941 agtcaaacgc atcatttcag cttgcttggc aaggtctagt gaagaaaagg ctagcgaatc
    30001 ttctgaacca aacaggcgta ctcgttcttc ctgactcaag ccttccagag gatagagaat
    30061 cttgtcatag ctagaattca gaaaccctgg aagcatggcc ttgaggatag agattttgta
    30121 ggagaagaca gatttgcgta actcctcagc cagccagagt tgttctggcg tgagaacagg
    30181 agaaaaatcc agcacctctg caatatcttt taaatcttgc tccatctctt ctccatctga
    30241 ttgggacttc aaaccaagaa caatcccttg aatcaggcga ttacccttac caaaaggcac
    30301 atgaacccgc atcccaactt ccagcattcc ctcaaattcc tccggaatcc tgtaactata
    30361 gggctggtcc gtctgcatca agggcacatc tacgataatc ttagctaggg ccatcttctc
    30421 acctcctcct tgtcagtaca ttcttgcaat agaaaaaata agattgagtc cccccaacct
    30481 taaatttttt caccatcttc tttttcttta gcaatttgct ctttgatttt cttttcttct
    30541 tcttctttgc ggcgtttttc ttcttcgata cggcgacgca ctgcttcacg ttttccttct
    30601 ggatctgggt gaattgtaac gtttcctgat tcgatttctt ctaaagcgcg aagagttgat
    30661 ttttcagact tgaaaccttg agttgctggg gcacctgctt ccaattcgtg ggcacgtttt
    30721 gcttccaaga ttacgagtga atattttgaa ggaaccttgt cgagcaaggt atcaatagag
    30781 ggttttaaca tcatttgctt gtacctattt tctaaatttt atcgggtagt tggagatttt
    30841 ggtaacatct cctgatagtg accaatgaca cgatccacac agaagtgttc tgcttcaatc
    30901 acacatttga cacgttcagc agctaggggc acctgatcgt tgacaatcgc ataatcatac
    30961 tcacgcatga gggcaatttc ttccttggcc ttttcgattc gttgggcaat cacttctgca
    31021 ctatctgttc cacgacctac caagcgatct tgcaattcat ccaaatctgg tggtgtcagg
    31081 aagataaaga cagcatctgg aacctttttc ttgacctgaa gagcaccctg aacttcaatt
    31141 tcaaggaaaa catcgattcc cttgtccaag gtttcattga cataggtcag aggagttcca
    31201 tagtagttgc cgacatattc tgcgtattcc aacatctgtc cttgacgaat cagctcttca
    31261 aattcttcac gagtacggaa gaaatagtca acaccgtcca cttctccagg acgttgtgcg
    31321 cgtgtcgtca tcgatacaga atattgaaat tggttttcag aactctcaaa aatctctctt
    31381 ctaaccgttc cttttccaac ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct
    31441 gccattgtgt ctccttttag tcaatctgtg aaataacatt tctctagaat aatggcaaaa
    31501 agccagatta tcctttacag tctttctatc tagtgtaaca aaaaagcagt aatttttcaa
    31561 ctgctctttc ttatttattt agcataatct actgcacgaa gctcgcgaat cacggttacc
    31621 ttgatatttc ctggataatc gagattgttt tcaattttct tacgaacttt gtgagccaag
    31681 attgtgactt tgtcgtcctt gatttttcct ggattgacca tgatacgaat ttcacgtcct
    31741 gcttgaaggg caaagctagt ttgcactcct tcaaagccgt tagcaatttc ttccaaatca
    31801 tggagacgct tgatgtagct ttcaagagac tcactacgag cacctggacg ggctgcgctc
    31861 aaggcatctg ctgcagcgac gataactgct atcacgctct cagcttcaac atctccgtgg
    31921 tgactagcaa tcgtattcac cacaactggg ggttccttgt acttacgggc caattccata
    31981 ccgatttcaa cgtggctacc ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg
    32041 aatccagcac gacgggcaag agccgcattt tcaccaagtt cgctcgccat gataccagcc
    32101 aacttagcaa cctcaatcga atggcgcaaa acattttgtc catatgaagt acggaactgc
    32161 aaacgtccca taatcttcat caagtctgga tgaaggtttg gcgcaccaat ttcataggca
    32221 gcagcctcac cgtattcacg aatcttattg tcaatctctt gacggttttt ctcaaccaac
    32281 tcttcgatac gagctggatg tatacgacca tctttgagca acatttccat agtcatacgg
    32341 gcaatctcac gacgaatcgg atcaaatcct gacaaggtca ccacttctgg tgtatcgtcg
    32401 ataatcacat cgacccctgt caaactttca aaggtacgaa tgttacgacc ttcacgacca
    32461 ataatgcgtc ccttcatagt atcgtctggc agatgaactg ttgagtttgt tgactccgct
    32521 acatattcac cagcgatacg ttgcatagct tgaaccaaga tgtccttggc cattttgtca
    32581 gaacgttcct tgacctcttg ctcagcttcg cgaatgcgac tggcaatctc cctggtcaag
    32641 ttttcctctg tctgagccaa gataatatct cgtgcttctg cctgagacag agcaccaata
    32701 cgctctagtt ctgcttcttt ttgtctttcg acttcctcta attgctcttc acgcgcatca
    32761 aggtttttcg ctctatcaga aatactttgt tctttttgtt caagtgtttg ttctttactc
    32821 gtcaaattgt cgtccttacg gtcaaggcta gtagctctct ctgtcaaacg actttcgatt
    32881 tgtttgagtt cttgacgttc tgatttgaat tcagcgtcca cttcttcacg gtattttctg
    32941 gcttcttctt tggcctccaa tagtgcttct tttttaagag acttgctttc acgtttagct
    33001 tcattaacaa gtaaatccgc ttcacgctca gcttgtccac gtaaattagt tgcttcttgt
    33061 tcagcattta aaagcatcaa ctccgcagct tcctgagatg atttcatctt agctgagatg
    33121 ctgacatatc caatgactaa accaatgatg acggcaaaaa cagcaatcgc aagcgacatg
    33181 atttccatgt ttttacctca ttttattgtt attccgaatg acatacattc ttttacattc
    33241 taccataaaa aagtgatttt cacaaaccta aaatagaata tgttttgggg aatttagtct
    33301 gaattttagg attacacttc cttggttttt taaggagata agtcagtgtc gctatcaaaa
    33361 agttccaccg aatttctaca actatggatt ttctattgtt gtgccaaata gtgttccact
    33421 agattcaaac attttgcttt caagtctttg aa
//
LOCUS       DAVLWA010000016        31874 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLWA010000016 DAVLWA010000000
VERSION     DAVLWA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.016"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            181..462
                     /locus_tag="VYN80_000714"
     CDS             181..462
                     /locus_tag="VYN80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1540661.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            594..1538
                     /gene="manA"
                     /locus_tag="VYN80_000715"
     CDS             594..1538
                     /gene="manA"
                     /locus_tag="VYN80_000715"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1540662.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1560..3035)
                     /locus_tag="VYN80_000716"
     CDS             complement(1560..3035)
                     /locus_tag="VYN80_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1540663.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3201..3365
                     /locus_tag="VYN80_000717"
     CDS             3201..3365
                     /locus_tag="VYN80_000717"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1540664.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3366..3872
                     /locus_tag="VYN80_000718"
     CDS             3366..3872
                     /locus_tag="VYN80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV1540665.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATATFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3892..4347)
                     /locus_tag="VYN80_000719"
     CDS             complement(3892..4347)
                     /locus_tag="VYN80_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1540666.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4367..5542)
                     /locus_tag="VYN80_000720"
     CDS             complement(4367..5542)
                     /locus_tag="VYN80_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1540667.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5601..6446)
                     /locus_tag="VYN80_000721"
     CDS             complement(5601..6446)
                     /locus_tag="VYN80_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1540668.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6571..7128
                     /locus_tag="VYN80_000722"
     CDS             6571..7128
                     /locus_tag="VYN80_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1540669.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7147..7614
                     /locus_tag="VYN80_000723"
     CDS             7147..7614
                     /locus_tag="VYN80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1540670.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7703..8332
                     /gene="upp"
                     /locus_tag="VYN80_000724"
     CDS             7703..8332
                     /gene="upp"
                     /locus_tag="VYN80_000724"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1540671.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8506..9096
                     /gene="clpP"
                     /locus_tag="VYN80_000725"
     CDS             8506..9096
                     /gene="clpP"
                     /locus_tag="VYN80_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1540672.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9175..9423
                     /locus_tag="VYN80_000726"
     CDS             9175..9423
                     /locus_tag="VYN80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1540673.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9524..10684
                     /locus_tag="VYN80_000727"
     CDS             9524..10684
                     /locus_tag="VYN80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540674.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10952..11821
                     /locus_tag="VYN80_000728"
     CDS             10952..11821
                     /locus_tag="VYN80_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1540675.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11825..12781
                     /locus_tag="VYN80_000729"
     CDS             11825..12781
                     /locus_tag="VYN80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1540676.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12781..13545
                     /locus_tag="VYN80_000730"
     CDS             12781..13545
                     /locus_tag="VYN80_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540677.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13545..14255
                     /locus_tag="VYN80_000731"
     CDS             13545..14255
                     /locus_tag="VYN80_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540678.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14669..15325
                     /locus_tag="VYN80_000732"
     CDS             14669..15325
                     /locus_tag="VYN80_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1540679.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15433..16528
                     /gene="prfB"
                     /locus_tag="VYN80_000733"
     CDS             join(15433..15504,15506..16528)
                     /gene="prfB"
                     /locus_tag="VYN80_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1540680.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16546..17238
                     /gene="ftsE"
                     /locus_tag="VYN80_000734"
     CDS             16546..17238
                     /gene="ftsE"
                     /locus_tag="VYN80_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1540681.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17231..18157
                     /locus_tag="VYN80_000735"
     CDS             17231..18157
                     /locus_tag="VYN80_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540682.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18443..20623
                     /locus_tag="VYN80_000736"
     CDS             18443..20623
                     /locus_tag="VYN80_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1540683.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20675..21490
                     /locus_tag="VYN80_000737"
     CDS             20675..21490
                     /locus_tag="VYN80_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1540684.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21630..22973
                     /locus_tag="VYN80_000738"
     CDS             21630..22973
                     /locus_tag="VYN80_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1540685.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23188..24378
                     /locus_tag="VYN80_000739"
     CDS             23188..24378
                     /locus_tag="VYN80_000739"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1540686.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24931..25866
                     /locus_tag="VYN80_000740"
     CDS             24931..25866
                     /locus_tag="VYN80_000740"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1540687.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25900..26937
                     /locus_tag="VYN80_000741"
     CDS             25900..26937
                     /locus_tag="VYN80_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1540688.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27078..27683
                     /locus_tag="VYN80_000742"
     CDS             27078..27683
                     /locus_tag="VYN80_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1540689.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27841..28371
                     /locus_tag="VYN80_000743"
     CDS             27841..28371
                     /locus_tag="VYN80_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1540690.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28392..29294
                     /locus_tag="VYN80_000744"
     CDS             28392..29294
                     /locus_tag="VYN80_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV1540691.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            29294..29776
                     /locus_tag="VYN80_000745"
     CDS             29294..29776
                     /locus_tag="VYN80_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1540692.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            29778..31319
                     /locus_tag="VYN80_000746"
     CDS             29778..31319
                     /locus_tag="VYN80_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1540693.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31374..>31874)
                     /locus_tag="VYN80_000747"
     CDS             complement(31374..>31874)
                     /locus_tag="VYN80_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1540694.1"
                     /translation="NQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNK
                     EILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPIFQTVFKTFL
                     KNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTN
                     FVLSRA"
BASE COUNT         9685 a         5928 c         6972 g         9289 t
ORIGIN      
        1 cctgttcttg tttcgtttta ctatagaacg atttgaaggc gtttatgata tttagctgta
       61 cgagagtctt taaaaagtgt tttgatggtt tggatttctt ctttagttga tttcatatca
      121 ctattatata atgctttttg attttagtat agtattatcg tagaaaatgg actcggtgct
      181 atggatacac cggatgagaa tggttatgta gcagatgact atcggattac ttacttagag
      241 gcccacatca aggccatgcg agatgccatt tacaaagacg gggttgactt gcttggttat
      301 acgacatggg gctgtatcga ttcggtttca gctggcacag gggaaatgaa taagcgctat
      361 ggctttatct atgtagatcg agataatgta ggaaatggga ctctcaaacg tagcaagaag
      421 aagtccttct attggtacat gtcatttata gcaatggtgt aagcatcgag tagagcacat
      481 ttgtttacta tttttataaa atcttctccc tgctttataa gatgtaaaaa agagttcaat
      541 tcgaactctt tttttgctat aatgagggga gaaaaatcag acaggagatt aagatgtcag
      601 aaccattatt tttacaatca gttatgcaag aaaaaatctg gggtggtacc aagctacgtg
      661 atgagtttgg ctacgacatc ccaagtgaaa aagtcggaga gtactgggct atctcagctc
      721 atctaaatgg tgtttctaaa gttgccaatg gtcgttacga gggaacagat cttgctactt
      781 tgtatgcgga acaccgtgaa ttatttggca atcgtcctga gcctgtattt ccgcttttga
      841 ctaagatttt ggatgctaac gactggctca gcgttcaagt tcacccagac gatgcttatg
      901 gactagagca tgaaggcgaa cttggaaaaa cagaatgctg gtacatcatc gctgcggatg
      961 aaggttcaga gattatctac ggccacaatg ccaagtcaaa agaagaactc cgccagcaaa
     1021 tcgaggataa gaactgggat gcgctgttga caaaagtgcc agttaaagca ggagatttct
     1081 tctatgtacc aagcggtacc atgcacgcta tcggggcggg tatcttgatt cttgaaaccc
     1141 agcagtctag cgataccact taccgtgtct atgactttga ccgtaaggat gacaagggca
     1201 atttacgtga acttcacctt gaaaaatcta tcgatgtctt gaacattggt gaacccgcaa
     1261 atagccgtcc agtgacaatc aaagcagatg atctacgttc cactctcctt gtatccaatg
     1321 atttcttcgc agtttacaaa tgggaaatta ctggaaaagt taactttgaa aagacagctg
     1381 actacagctt gttgagtgtc ttggctggtc aaggtcaact aactgttgac gggaaaaact
     1441 atccaatcca aaaagggaga cactttatcc taccaagtga tgttgaagct tggactctgg
     1501 aagggcaaga tttagaatta attgttagtc atccataaaa aagaaagggc ctgagttcat
     1561 tactcaagtc ctttttgatt acataaattg ggcgatgaag accacgatga tgattggaat
     1621 gacggaacga agaaacgaag aaggaagagc cagacttgga acagtccttg tttccatgct
     1681 ctttcatcga gatggagttc ctccattgca agagcctttt taaagatata gcctgtaaaa
     1741 agtgaaaggt agagagctcc aaatggcatg aggagattgg gaaccaagaa gttcatagcg
     1801 tcaaagaagg tcttaccaaa aatgtgaaca tccgccatga caccgtaaga tagggctgaa
     1861 ggaatgccaa agacaaaggt caaaattcct aaaataacac tccatttggc acgtttgctg
     1921 ttatcctggt tggtgatatt gcccacattg atctccagca tcacgacaga agaagtgaca
     1981 gtcgcaaaga ggaagaggac gtagaaaatg gttccaaaag gcatcttgtc aaagagttga
     2041 ggcaagacga taaagagcaa gctcggtccc ccttcagact ggatattgaa gggggaccga
     2101 gcttggaaaa tggctagacc tgccatgatg gatatcgaga tattcatggc tacgatggag
     2161 attcctgact ggactagatt ggttttcttg tccaagtaag aagcataggt caacatgact
     2221 gtaaccccta gtgagagggc aaagaaagat tgtcccagag catagaggag accagcgcta
     2281 gtcagttttg aaaagtctgg tttgaggaag taaagaaccc cttccatggc atttggcaaa
     2341 ctgagagagc gaccgatgat aacaacaaag atgataaaga gcaggggcat catgactttc
     2401 gaagctcttt caatcccttt ttgaacccca cgtgatacaa taaagatatt taataagata
     2461 aaggctgctt gagctcctag ggcaatggct ggatttgaaa tgattgaagt aaataactga
     2521 gcataatcac ccgttccacc aagttggaac aatttcccaa actcaatacc tagatagact
     2581 agaatccatc ctccgataac actgtaaaaa gataagagga taaagagggc aaaggcgcca
     2641 atccaaccga taaagttgta cttgttattc ttgcccagtt ttccaaaggt tttgatagcg
     2701 gaaacgccag cactacggcc aagggcaaac tcagccagca ggagagggaa accgattaaa
     2761 atagtggaaa tgagaaagat tagtaaaaag cctccaccgc cattagcagc agtcatgtag
     2821 ggaaacttcc aaacggaacc aagcccgatg accgagccag cagatgctag aataaagcca
     2881 agtttcgacc cccattgtga tttttctgac atagttaaac tctgatctcc tttttcaaaa
     2941 taagaaaaag ctacttgagt tgtcaaatag ccttctgttt gattgataga cttgactatc
     3001 ctattatgtt ttctaaggtc tgtcaagaaa accattttca agttttcaca cctttctcaa
     3061 aaacgcttga ctctgaccta aggggagggg ttatactatc attgtaagga ggaaatcatg
     3121 taccatataa aagaagctgc gcagctttca ggtgtctctg tcaagacccc gccctgcatc
     3181 actatgacaa gataggactc ttggtcccct taaagtcgga aaacggctat cgaacctata
     3241 gtcaagagga tttggaacgc cttcaggtca ttctttacta caaatatcta ggcttttctt
     3301 tagagaaaat agcagaactg ttaaaggaag aaaggacaga tttattgctc catttgacta
     3361 ggtagttgga ctatctaact cgcgaaaggc aacatctgga taccttgatt tccaccttgc
     3421 aaaaaactat tcaagaacaa aaaggagaaa gaaaaatgac cattgaggaa aaattcacgg
     3481 gatttagcta tcaagacaat caaaaatacc accaagaagc ggtagagaaa tatggtcaag
     3541 aagtcatggg acaagcgctc gaacgccaaa aaggtcacga agacgaggct acggccacct
     3601 ttaaccaagt ctttcaaact ttggcacaaa atcttcaagt tggtttacct gcaacagcaa
     3661 ccgaaaacca ggagcaagca gccaagctct tgcaagccat tcgcacttat ggatttgact
     3721 gctctattga ggtattcggt catatcggta aaggttacgt ctacaaccca gagtttaagg
     3781 aaaacattga caagtttggt tctgaaacag cccagtacac gtcagatgcc attgcggctt
     3841 acgttcagac aaatgcagaa aaaataggct aggaatttcc tagcctattt tttacttcaa
     3901 atcataaagc cagtcgtcac cgtttttgta gtaaaagaat tcactgagat cttcttctag
     3961 aaacacacga agcatatcag acatatcatc ggttgcaagt tttagatgag aaagattttc
     4021 aaagtcctcc caccaaactt tcccttcgtc tgaagactgg agttcaccag taaagtgttc
     4081 tgtcttgtaa aaaaggacga cataacgata atccttgtcg tcataccagt ttttgatacc
     4141 acagagttgg ggtttggaaa tgatcagacc agtttcttct ttcacttcac gaatgacagc
     4201 atcgacaaag gattcgccac gttcaacatg accaccagga aaagtaatgc cagaccagtc
     4261 gggactaact cggtcttgga ccaggacctt atctccgttt ttaatcatac acatgttaac
     4321 aaattcgact gcctctcttc tgttcattct tcacaacctt taatctttaa tcataatgca
     4381 gacttcccgc cacccagccg gtacagaggg cagaagtgat gttaaagcca cccgtgtggg
     4441 cattgatatc cataacttcg cctgcaaagt ggaggccagg taccagctta ctttcaaggg
     4501 ttttaggatt gatttccttg agactgactc cacccttggt aacaaaggac tttgcaaggg
     4561 acatttttcc agttacagga attttaagtt ctttaatgga ctggacaagt tgttctcgtt
     4621 ccttttcagt cagttgtttg actttttcag gatatccttg tacaaaaaat tcggccaagc
     4681 gttctggtaa caaggttttt aaagcgtttt tcaaggattt ttcccgattt tcttctagaa
     4741 atgtaaccaa gtccttctca gaaagttgag gcaaaacatc gagtgagaga acctccccac
     4801 ctttgacaaa gctagacatg cgtagggcag caggacctga caaaccaaag tgggtaaaga
     4861 gtaaatcatg agtgatgaca tgcttaccat aacttagggt cacatcgtcc agagaaatcc
     4921 cttgtaaggc tttatgtgga aaatctgtta ataaaggact ttcagcagcc tcaagatcgg
     4981 tgatggtatg cttaaaatgg cgagcaatct cgtgaccaaa accagtcgaa ccagtcgaag
     5041 gataagactt accacctgtt gtgacaatga gtttctcaca agtgaaggtt tgatccgctg
     5101 acttaaggac aaactggtca tctacttttt taacagaaac gatttctatt tgagtagcaa
     5161 cttgaccacc tagttcggtg attttctttt ccaaagcttc gataatagtc cgagacttgt
     5221 cactggctgg aaagacgcgt ccgtggtctt cgaccttaag tttaacacca ttttctgtaa
     5281 aaaagttgat gatgtcatga ttatcgaact gggagaaaac actgtaaaga aagcgtccgt
     5341 ttccaggaat tccagctagc aggttgtcta agctaccatt gttggtcaca ttgcaacgtc
     5401 ccccaccagt cccagctaat ttttttccaa gtttccgatt tttttcgatg aggagggttt
     5461 tctgtccata aaagctactg gaaatcgtag ccatcatacc agcaggtccc ccaccgatga
     5521 caatagtatc aaaatgtttc atagctctat tgtaccacaa aaaaacaaga gatgatggtc
     5581 acctcttgtc aagaatgcaa ttaatcaatt tcatagccca tcagcaaacc gccctcttct
     5641 gcatagaaac tgcagagacc agaggttggt agaattttaa tatccgcttg tgggaaggtt
     5701 tcacggattc gctctgagag ctgttgacaa catttttcgt tattgcgttg ggccatgaca
     5761 atacggccac cagcatatcc agcttttact aactcatcat aggcagcttg aactgatttc
     5821 tttgatcccc ttgctttttg tagcaattcg agagttccag tttcactagc ttttccgacc
     5881 atacgaatgt tgagaaggcc aacgaccgta ccgataagct tgctcaaacg gccgttcttc
     5941 accaagttat cgactttggc taggacaaag agcaacttag ttttttcttg ataggcagtg
     6001 atagcttcaa ccacttcttc aaaagacaag ccctggtcaa tcaagtcatt caatttttct
     6061 acgagtaggt caacttcacc accagcagat aaactatcaa tcacatgaat cttagtgtca
     6121 ggatggtctt ctagataaat attctttgct agttgagcac tattgtgact gccagaaagg
     6181 gtacctgtga tggttactag gaaaatgttt ttggcacctt caaatgctcg caaatagtca
     6241 tctgggcttg gacaagccga ttttgaagct tctgcagttg catacatggt ttccatcatt
     6301 tggtcaatat cgagactggc gtcatcaaca aagacctgat cagctacttg aatggttaag
     6361 gggacactta caaaggttgt gttaatagct ggtgttggca gttgacgata atcacaacca
     6421 gagtcagcaa taattttcca agtcatagaa attctccatc tttgtcagtt atacattgac
     6481 aaaggttctg tcttttttta caattatatc atgaaaaccc ttgaaacaaa agcatcatcc
     6541 gtctgttgtg acaagtagaa agaaaatgtt atgtctgaac gtagaatctc tgaaaagtct
     6601 cttgaaaatc tcagaaaatc aaaccaagaa tccaatttat taaccagaga agccattgaa
     6661 acagccctct tgcaactctt ggaaaaaaag gagctgacca agattagtat ttctgaattg
     6721 gtcaaacgtg caggtgtttc gcgtgcggcc ttttatcgca attatgactc caaagaggaa
     6781 attttagaga gcgtctttaa acgaactgtc cacaatatta tggaacagct gcatcattac
     6841 gatttaaaga cagaccttta tttggtttgg gttcaccttt tccgggaggc cagaaaggaa
     6901 gctagagtga ttcaattggc cttggattac catctggaaa aaatctttgt ccaagccatg
     6961 caggaatttc tagaaaaata ccatgggaaa tcaaaaggtg tcagctctta tcttcattcc
     7021 ttctggagct cagccatcgt ctctgtcctt ctaaaatgga tcaaggatgg catgaaggta
     7081 ccagctgaaa agattgcaga tttaggttta ccatttttta aaaaatagag aaaaaggaga
     7141 aaagagatga ctgaaaaaag actggcctgg gatgagtatt ttgcagccca agccctacta
     7201 attgcgaatc gttccacttg taaacgtgct aaagtgggcg cgattctggt aaaagataat
     7261 aaggttattt ccactggtta caatggttcg gtgtcaggga ctgagcattg tattgatcac
     7321 gaatgtttgg tcattgaagg ccactgtgtt cgcacccttc acgctgaggt caatgctatc
     7381 cttcaaggtg ctgagcgtgg tgttcccaaa ggctttacag cctatgtaac ccattttcct
     7441 tgtctcaact gtacaaaaca attgcttcag gtcggctgca agcgcgtggt ttatatcaac
     7501 cagtaccgaa tggacgacta cgcccaatac ctttatcaag aaaaagggac agaattgacc
     7561 catttaccac ttgagacagt acagacagct cttaaagagg ctgatctaat gtaaaaatta
     7621 tcaaaaataa atggcttaga aagattttta aaccgttttt tggtataata agaagaataa
     7681 attgaaagaa ggaattccaa aaatgggaaa aattgaagtt attaatcacc cactgattca
     7741 acacaaattg tcaatcttgc gtcgtacaga tacttctaca aaagcttttc gtgagctagt
     7801 agatgagatt gcaatgttga tggggtatga agtacttcgt gatcttccac tagaagatgt
     7861 ggaaatcgaa acaccaatta caaaaacagt tcaaaaacaa ttggcaggta agaaattggc
     7921 catcgtccca atcttgcgtg caggtatcgg gatggttgat ggtctcttga gcttggttcc
     7981 agctgctaaa gttggccaca tcggtatgta ccgtgatgaa gaaacacttc aaccagttga
     8041 atacttggtg aaattgcctg aggacattga ccaacgtcaa atttttatag tagacccaat
     8101 gttggcaaca ggtggctcag caatcttggc tgttgattct cttaaaaaac gtggcgcatc
     8161 aaatatcaaa tttgtctgcc ttgtatctgc tccagagggt gtaaaagccc ttcaagaagc
     8221 tcatccagat gtagaaatct ttacagcagc cttggatgaa cgtttgaacg aacacggtta
     8281 tatcgttcca ggtcttggag atgctggaga ccgcttgttc ggtacaaaat aagatcgaaa
     8341 agaagaatga cttggaaaag tatttccagt cacgaaagga ggttgggttt ttgtttctgt
     8401 ctaatgaaag cagagcaaaa atttgacctt ttttgaccaa gatattataa tagtcttatc
     8461 ttcagtcatt tgaccaacaa aattaaaact caaaaggaga aatgaatgat tcctgtagtt
     8521 attgaacaaa caagccgtgg agaacgttct tacgatattt actcacgtct tctcaaagac
     8581 cgcatcatta tgctgacagg tccggttgaa gacaatatgg ctaactctgt tattgcccaa
     8641 ttgcttttct tggatgccca agatagtaca aaagatattt acctttatgt caatacacca
     8701 ggtggttctg tttcagctgg tttggcaatc gtagatacca tgaactttat caaggcagat
     8761 gtccaaacca ttgttatggg aatggctgca tctatgggaa cagtcatcgc atcaagtgga
     8821 gcaaaaggca aacgtttcat gcttccaaat gctgaataca tgattcacca accaatgggc
     8881 ggtacaggtg gtggtaccca acaaactgat atggctatcg ctgcagaaca cttgctcaaa
     8941 actcgtaata ccttggaaaa aatcttggct gaaaattcag gtcagtcaat ggaaaaagtc
     9001 catgcagatg cagaacgtga taactggatg agcgcccagg aaacacttga atatggcttt
     9061 attgatgaaa ttatggccaa caattcattg aactaatgat gatagaaggc aaactcgact
     9121 gggtttgctt tttttggtat aatagggaga gatttcttag aaagaggatt tatcatgttt
     9181 gaaaaagtca atcgctctgg cttgattatc tatctttact ataatcgtga tgccaaaaaa
     9241 ctgcaggatt atggagatat tacctatcat tccaagaaac atcgttactt acaactctat
     9301 gttccaactc aagaagtgga gcaattggtc ggacgcttga gcaaggaaaa atttattaaa
     9361 aaagttaggg tttgtcatat ccaagagttg gaaacaccct ttgtgggcaa tctttatcga
     9421 taggaaaacg ttatcatcga aaaagttcaa ggaaaacatt gacaattttc tgataattcg
     9481 gtatattctt aacagactat ttaagaaata aggagacaaa aagatgaaga aaaaatttgc
     9541 cctatcgttt gtggcgcttg caagtgtagc acttcttgca gcctgtggag aagtgaagtc
     9601 tggagcagtc aacactgctg gtaactcagt agaggaaaag acaattaaaa tcgggtttaa
     9661 ctttgaagaa tcaggttctt tagctgcata cggaacagct gaacaaaaag gtgcccaatt
     9721 ggctgttgat gaaatcaatg ccgcaggtgg tatcgatgga aaacaaatcg aagtagtcga
     9781 taaagataat aagtctgaaa cagctgaggc tgcttcagtt acaactaacc ttgtaaccca
     9841 atctaaagta tcagcagtcg taggacctgc aacatctggt gcgactgcag ctgcggtagc
     9901 gaacgctaca aaagcaggtg ttcccttgat ctcaccaagt gcgactcaag atggattgac
     9961 taaaggtcaa gattacctct ttattggaac tttccaagat agcttccaag gaaaaattat
    10021 ctcaaactat gtttctgaaa aattaaatgc taagaaagtt gttctttaca ctgacaatgc
    10081 cagtgactat gctaaaggga ttgccaaatc tttccgcgag tcatacaagg gtgaaatcgt
    10141 tgcagatgaa actttcgtag caggtgacac agacttccaa gcagccctta caaaaatgaa
    10201 agggaaagac tttgatgcta tcgttgttcc tggttactat aatgaggctg gtaagattgt
    10261 aaaccaagcg cgtggtatgg gaattgacaa accaatcgtt ggtggtgatg gattcaacgg
    10321 tgaggagttt gtacaacaag caactgctga aaaagcatca aacatctact ttatctcagg
    10381 cttctcaact actgtagaag tttcagctaa agctaaagcc ttccttgacg cttaccgtgc
    10441 taagtacaat gaagagcctt caacatttgc agccttggct tatgattcag ttcaccttgt
    10501 agcaaacgca gcaaaaggtg ctaaaaattc aggtgaaatc aaggataacc ttgctaaaac
    10561 aaaagatttt gaaggtgtaa ctggtcaaac aagcttcgat gcagaccaca acacagtcaa
    10621 aactgcttac atgatgacca tgaacaatgg taaagttgaa gcagcagaag ttgtaaaacc
    10681 ataatagaaa aatgttgaaa tagggaatga gcctttgact cactccctgt ttcgatattt
    10741 aatactcttc gaaaatctct tcaaactgcg tcaacgtcgc cttggattat atatgtgact
    10801 gacttcgtca gtcttatcta caacctcaaa gcagtgcttt gagcaacctg cggctagttt
    10861 cctagtttgc tctttgattt tcattgagta taagaaccta tcaaaaagtg agggaaaccc
    10921 ctcggaatta taaatagaaa gagtgaatct tatgctccaa caactcgtaa atggtttgat
    10981 tctaggtagt gtttacgcgc tgttagccct aggatatacc atggtttacg gaattatcaa
    11041 gctcatcaac ttcgcccatg gtgatattta tatgatggga gcctttatcg gttatttctt
    11101 gatcaattct ttccaaatga atttctttgt agcgcttatt gtagctatgc tagcgacagc
    11161 tattcttggt gtcgtgattg agtttcttgc ttaccgacct ttgcgccact ctactcgtat
    11221 tgctgttttg attacggcta ttggggtttc tttcctattg gagtatggaa tggtctatct
    11281 ggttggtgcc aatacccgtg ccttccctca agcgattcaa acagttcgat atgatttggg
    11341 accaattagc ttaacaaatg tgcagttaat gattttggct atttccttga ttttgatgat
    11401 tttgttacaa gtcattgtcc aaaagactaa gatggggaaa gccatgcgtg cagtatcagt
    11461 agatagcgac gcggcgcaat tgatggggat caatgtaaac cgtacgatta gctttacctt
    11521 cgctttgggt tctgctcttg cgggtgcggc tggtgttctg attgctcttt attataactc
    11581 tcttgagcct ttgatggggg ttactccagg tcttaaatct ttcgttgccg cagtacttgg
    11641 tggtatcgga attattcctg gtgcggctct tggtggcttt gtgattggtc tattggaaac
    11701 ctttgcgact gcctttggga tgtcagattt ccgtgatgcc attgtttatg gaatcttgtt
    11761 gttgatcttg attgtccgcc cagctggtat ccttggtaag aatgtgaaag agaaggtgta
    11821 aacgatgaag gaaaatttaa aagttaatat tctatggtta ctccttttgt tagctggcta
    11881 tagcttgatt agtgtactgg tttcagtcgg agtacttaat ctattctatg tacagatttt
    11941 acagcaaatt ggaattaata ttattttggc tgttggtctc aacttaatcg ttggtttttc
    12001 aggacaattt tcacttggtc atgctggttt catggcgatt ggtgcctatg cagcagctat
    12061 tattggttct aaattaccaa cctacggtgc cttctttgga gctatgcttg taggggcttt
    12121 gctttcagga gcagttgcct tacttgtcgg cattccaacc ttgcgcttga agggggacta
    12181 tcttgcggta gcaactctgg gtgtttctga aattatccgt atctttatca tcaatggtgg
    12241 aagccttaca aatggtgcgg caggtatctt agggattcct aactttacaa cttggcaaat
    12301 ggtttacttc tttgtcgtga ttacaaccat tgcaaccttg aacttcttgc gtagcccaat
    12361 tggtcgttca accctctctg ttcgtgaaga tgaaatcgct gctgagtcag ttggggttaa
    12421 tacgactaaa attaaaatca tcgcttttgt ctttggtgcc attactgcaa gtattgctgg
    12481 gtcacttcag gcaggattta tcgggtctgt tgtaccgaaa gattacacct tcatcaactc
    12541 aatcaacgtt ttgattattg ttgtatttgg tggactcggt tccattacag gtgcgattgt
    12601 ttcggctatt gttctgggaa ttttgaatat gcttctccaa gatgttgcta gtgtgcgtat
    12661 gattatttac gctttggcct tggtattggt aatgattttc agaccaggtg gactccttgg
    12721 aacatgggaa ctgagcctat cacgtttctt taaaaaatct aagaaggagg aacaaaacta
    12781 atggcattac ttgaagtaaa acagttaacc aaacattttg gtggtctaac agctgttgga
    12841 gatgtgactc ttgaattgaa cgaaggggaa ctggttggat taatcggtcc aaacggagct
    12901 gggaaaacca cccttttcaa ccttttgacc ggtgtttatg aaccaagcga gggaacagta
    12961 accctagatg gtcacctttt gaatgggaaa tcaccttata agattgcctc tttgggactt
    13021 ggacgtactt tccaaaatat ccgtctcttt aaagatttaa cagttttaga caatgttttg
    13081 attgcttttg gaaaccatca caaacaacat gtttttacta gtttcttacg cttaccagct
    13141 ttttacaaga gtgaaaaaga attaaaggct aaagctttgg aattgttgaa aatctttgat
    13201 ttagatggtg atgcagagac tcttgctaaa aatctttcct acggacaaca acgtcgtttg
    13261 gaaattgttc gtgcccttgc tacggaacct aaaattctct tcttagatga accagcagca
    13321 ggtatgaacc cacaggaaac agccgaattg actgagttaa ttcgtcgtat caaagatgaa
    13381 tttaagatta caatcatgtt gattgaacac gatatgaatc tggtcatgga agtaacagaa
    13441 cgtatctacg tacttgaata tggccgttta attgctcaag gaactccaga cgaaattaag
    13501 accaataaac gcgttatcga agcttatcta ggaggtgaag cctaatgtct atgttaaaag
    13561 ttgaaaatct ttctgtgcat tacggtatga tccaagcagt ccgtgatgta agctttgaag
    13621 ttaatgaagg agaagttgtt tcccttatcg gtgccaacgg tgcaggtaag acaactattc
    13681 ttcgcacctt gtcaggtttg gttcgaccaa gttcaggaaa gattgaattt ttaggtcaag
    13741 aaatccaaaa aatgccagct cagaaaattg tggcaggtgg tctttcacaa gttccagaag
    13801 gacgccacgt ctttcctggc ttgactgtta tggaaaatct tgaaatggga gctttcttaa
    13861 agaaaaatcg tgaagaaaat caagctaact tgaagaaggt tttctcacgc tttcctcgtc
    13921 ttgaagaacg taagaaccaa gatgcagcta ctctttcagg aggggaacaa caaatgcttg
    13981 ccatgggacg cgctcttatg tcaacaccaa aacttcttct tttagatgaa ccatcaatgg
    14041 gacttgcccc aatcttcatc caagagattt ttgatatcat tcaagatatt cagaagcaag
    14101 gaacaaccgt cctcttgatt gaacaaaatg ccaataaagc acttgcaatc tctgaccgag
    14161 gatatgtact ggaaacaggg aaaatcgtcc tatcaggaac aggaaaagaa ctcgcctcat
    14221 cagaagaagt cagaaaagca tatctaggtg gctaaaacaa tccagtggat tgttttagtc
    14281 ggcagatgaa gattacgaag taatcatcat tatagtctgg gagactagtt taggttgcag
    14341 ataaagattg cgaagcaatc atcaaatcca gtggattgtt ttagtcggca gatggagatt
    14401 acgaagtaat catcaatata gtccggggga cctttttagt cggtagattg agattgcaaa
    14461 caaatctgca tctacattga aagcttaatt tctaataatt gaaaaaatcg aatgaaaaat
    14521 ttcttacctt cattcacaga gctcgatttc agagctcttt ttgctagctt attcatactt
    14581 ttctgaattt cgaaaaagaa atgtaagcgt ttgatagatt tacaaaaaga ttgtataata
    14641 gggataagaa tagaaaagga gaagtctcat ggcagttaaa gattttatga cccgcaaggt
    14701 agtttatatt agtccagata caacagtatc tcatgcagca gatttgatga gagagcaagg
    14761 tttgcaccgt ctgcctgtta tcgaaaatga tcaattagtt ggtttggtga ctgagggaac
    14821 cattgcacaa gcaagtccat ctaaagcaac aagtctttct atctatgaga tgaattatct
    14881 tctgaataag acaaaagtaa aagatgtcat gattcgcgat gttgtcactg tctcaggcta
    14941 tgctagtcta gaagatgcaa cttatctgat gttgaaaaat aagattagta ttctccctgt
    15001 cgtagataac catcaagtat acggagttat tactgaccgt gacgttttcc aagcctttct
    15061 tgaaattgca ggttatggcg aagaagggat tcgtgtacgc tttgttacag aagatgaagt
    15121 tggtgttctt ggaaaaattg tttctttgat tgtagaagaa aatttgaata tctcccatac
    15181 agtcaatatt ccgcgtaagg atggtaaggt gattatcgaa gtgcaaatcg atggatcaat
    15241 tgatttacca gccttgaaag aaaaatttga agcaaatggt attcaagtgg aagaaatcgc
    15301 tcgcacttca gcaaaagtct tgtaagaagg gaagcccaaa ggcttctttt ttcatgaaaa
    15361 ggggattaga gaaaaagatg gaaagaaatg ataaaatatg ctataatgaa ataatgtaaa
    15421 aaaggagtat ttatggacat ttcagtaatt cgtcagaaaa ttgacgcaaa tcgtgaaaaa
    15481 ttagcttctt tcagggggtc tctttgacct cgaagggcta gaggaagaga ttgccatctt
    15541 ggaaaacaag atgacagaac ctgatttttg gaacgataat attgcagccc aaaaaacgtc
    15601 gcaagaatta aatgaattaa aaaacactta caataccttc cataagatgg aagagttgca
    15661 ggatgaagtc gaaattttat tggatttttt ggctgaagac gagtcagtgc atgatgaact
    15721 ggtagcgcag ttagccgaac ttgataagat aatgaccagc tacgagatga ctctactctt
    15781 gtcagaacct tatgaccaca acaatgccat cttggaaatc catccaggtt ctggtggtac
    15841 tgaggcgcag gactggggtg atatgttgct tcgtatgtat actcgttatg gtaatgctaa
    15901 aggctttaaa gtggaagtgt tggattacca agcaggtgat gaggctggta ttaagtcggt
    15961 aactttatca tttgaagggc ctaatgccta tggtctcctc aagtcagaaa tgggtgttca
    16021 ccgcttagtg cgaatctcac catttgactc tgccaaacgt cgccatacct ctttcacatc
    16081 tgtagaagtg atgccagaat tggatgatac tattgaagtg gaaatccgtg aagatgatat
    16141 caagatggat accttccgtt caggtggtgc cggtggacaa aacgtcaata aggtttcaac
    16201 aggtgtacgt ttaacccaca ttccaactgg aattgttgtc caatcaacag tggatcgtac
    16261 ccagtatgga aatagagatc gtgccatgaa gatgttgcag gctaagctct atcaaatgga
    16321 gcaagagaag aaggctgcag aggtagattc tctcaaaggt gagaaaaagg agatcacttg
    16381 gggaagccaa atccgttctt atgtcttcac gccttatact atggtaaaag atcaccgaac
    16441 tagctttgag gttgctcagg tagataaggt tatggatggg gacttagatg gttttatcga
    16501 tgcttatctc aagtggcgaa ttagctaaga tagaaaggaa ctcacatgtc aattattgaa
    16561 atgagagatg tcgttaaaaa atacgacaac ggaacgactg ctctacgcgg tgtttcggtt
    16621 agcgttcaac cgggggaatt tgcttacatc gtaggacctt caggagcagg gaagtcaact
    16681 tttattcgtt ctctgtatcg tgaagtaaaa atcgataaag gaagcctatc agttgctggt
    16741 tttaatctgg ttaagatcaa aaagaaagat atcccgcttc tacgtcgtag tgttggggtt
    16801 gtcttccagg attataaatt gttaccaaag aaaactgtct atgaaaatat tgcttacgct
    16861 atggaagtaa tcggggaaaa tcgccgtaat atcaaaagac gagtgatgga agttttggac
    16921 ttggttggat tgaagcataa ggttcgttct ttcccaaatg aactctcagg tggggagcaa
    16981 cagcggattg cgattgcgcg tgcaattgta aataatccca aagtattgat agctgatgag
    17041 ccaacaggaa atctggatcc ggataattca tgggaaatta tgaatctttt ggaacggatt
    17101 aacctacaag gaacaactat tttgatggcg actcataata gccagattgt aaataccttg
    17161 cgccaccgtg tcattgccat tgaaaatggc cgtgtcgttc gtgacgaatc aaaaggagag
    17221 tatggatacg atgattagta gattttttcg ccatttattt gaagccttaa aaagtttgaa
    17281 acgaaatggt tggatgacag tagctgctgt cagttcagtc atgattactt tgaccttggt
    17341 ggcaatattt gcatctgtta ttttcaatac agcgaaacta gctacagata ttgaaaataa
    17401 tgtccgtgta gtagtttata tccgaaagga tgtggaagat aatagtcaga caattgaaaa
    17461 agaaggtcaa actgttacaa ataatgacta ccacaaggta tatgattctt tgaagaacat
    17521 gtctacggtt aaaagtgtta ccttttcaag taaagaagaa caatatgaaa aattaaccga
    17581 gataatggga gataactgga aaatctttga aggagatgcc aatcctctct atgatgccta
    17641 tattgtagag gcaaacactc caaatgatgt aaaaactata gccgaagatg ctaaaaaaat
    17701 tgaaggtgtc tctgaggttc aagatggcgg tgccaataca gaaagactct tcaagttagc
    17761 ttcatttatc cgtgtttggg gactagggat tgctgctttg ttaattttta tcgcagtttt
    17821 cttgatttca aataccattc gtattaccat tatttcccgc agtcgcgaaa ttcaaatcat
    17881 gcgcttggtc ggagctaaaa acagttatat ccgtggaccg ttcttgttag aaggagcctt
    17941 tatcggttta ttgggagcta tcgcaccatc tgttttggtc tttattgttt atcaaattgt
    18001 ttaccaatct gtcaacaaat cgttggtagg gcaaaatcta tccatgatta gtccagattt
    18061 atttagtccg ttgatgattg ccctactatt tgtgattggg gttttcattg gttcattggg
    18121 atcaggaata tccatgcgcc gattcttgaa ggtttaggta aaatagctgc ttttatgagg
    18181 agattgtaaa atctcctttt ttgctacaag agtttttgaa aagagatgcg cagaagaaaa
    18241 gagcttccaa agaagtcccc cagagaagac ttcttgatgg ctaatttgaa cttgaaattt
    18301 ggcgcactag aaatttttta gaaaaaagtg taattttttt ataaaagcct ttacaaaaaa
    18361 aattaaagtg gtataattaa atcatagaaa gtgcaaacgt tttcgtaaaa cgtttgccta
    18421 aataaaaata aaggagattt gaatgatgaa agatacattc aaaaatgtct tgtctttcga
    18481 attttggcaa aaattcggta aggctttgat ggtagttatc gcggttatgc cggctgctgg
    18541 tttgatgatt tcaatcggta agtctatcgt gatgattaac ccaacctttg caccacttgt
    18601 catcacaggt ggaattcttg agcaaatcgg ttggggggtt atcggtaacc ttcacatttt
    18661 gtttgcccta gccattggag gaagctgggc taaagaacgt gctggtggtg ctttcgccgc
    18721 tggtcttgcc ttcatcttga ttaaccgtat cactggtaca atctttggtg tatcaggcga
    18781 tatgttgaaa aatccagatg ctatggtaac tactttcttt ggtggttcaa tcaaagttgc
    18841 tgattacttt atcagtgttc ttgaagctcc agccttgaac atgggggtat tcgtagggat
    18901 tatctcaggt tttgtagggg caactgctta caacaaatac tacaacttcc gtaaacttcc
    18961 tgatgcactt tcattcttca acgggaaacg tttcgtacca tttgtagtta ttcttcgttc
    19021 agcaatcgct gcaattctac ttgctgcttt ctggccagta gttcaaacag gtatcaataa
    19081 cttcggtatc tggattgcca actcacaaga aactgctcca attcttgcac cattcttgta
    19141 tggtactttg gaacgtttgc tcttgccatt tggtcttcac cacatgttga ctatcccaat
    19201 gaactacaca gctcttggtg gtacttatga cattttaact ggtgcagcta aaggtactca
    19261 agtattcggt caagacccac tatggcttgc atgggtaaca gaccttgtaa accttaaagg
    19321 tactgatgct agtcaatatc aacacttgtt agatacagta catccagctc gtttcaaagt
    19381 tggacaaatg atcggttcat tcggtatctt gatgggtgtg attgttgcta tctaccgtaa
    19441 tgttgatgct gacaagaaac ataaatacaa aggtatgatg attgcaacag ctcttgcaac
    19501 attcttgaca ggggttactg aaccaatcga atacatgttc atgttcatcg caacacctat
    19561 gtatcttgtt tactcacttg ttcaaggtgc tgccttcgct atggctgacg tcgtaaacct
    19621 acgtatgcac tcattcggtt caatcgagtt cttgactcgt acacctattg caatcagtgc
    19681 tggtattggt atggatatcg ttaacttcgt ttgggtaact gttctctttg ctgtaatcat
    19741 gtactttatc gcaaacttca tgattcaaaa attcaactac gcaactccag ggcgcaacgg
    19801 aaactacgaa actgctgaag gttcagaaga aaccagcagc gaagtgaaag ttgcagcagg
    19861 ctctcaagct gtaaacatta tcaaccttct tggtggacgt gtaaacatcg ttgatgttga
    19921 tgcatgtatg actcgtcttc gtgtaactgt taaagatgca gataaagtag gaaatgcaga
    19981 gcaatggaaa gcagaaggag ctatgggtct tgtgatgaaa ggacaagggg ttcaagctat
    20041 ctacggtcca aaagctgaca ttttgaaatc tgatatccaa gatatccttg attcaggtga
    20101 aatcattcct gaaactcttc caagccaaat gactgaagca caacaaaaca ctgttcactt
    20161 caaagatctt actgaggaag tttactcagt agcagacggt caagttgttg ctttggaaca
    20221 agtaaaggat ccagtatttg ctcaaaaaat gatgggtgat ggatttgcag tagaacctgc
    20281 aaatggaaac attgtatctc cagtttcagg tactgtgtca agcatcttcc caacaaaaca
    20341 tgcttttggt attgtgacgg aagcaggtct tgaagtattg gttcacattg gtttggacac
    20401 agtaagtctt gaaggtaaac catttacagt tcatgttgct gaaggacaaa aagttgcagc
    20461 aggagatctc cttgtcacag ctgacttgga tgctatccgt gcagcaggac gtgaaacttc
    20521 aacagtagtt gtcttcacaa atggtgatgc aattaaatca gttaaattag aaaaaacagg
    20581 ttctcttgca gctaaaacag cagttgctaa agtagaattg taatatactt gaggttggaa
    20641 gctgtattcc aacctcttat tttgggagaa aagaatgaaa tttttaacac tcaatactca
    20701 cagttggatg gagaaagaag cagaggaaaa attccagatt ttgcttgaag atattcttga
    20761 aaaggactat gatttgattt gtttccaaga aatcaatcag gagatgacct cgtcagaggt
    20821 ggaggttaat gacctttatc aagctttgcc agcagctgag cctattcacc aagaccatta
    20881 tgttagactc ttggttgaaa agttgttaga gcaagggaaa aattactact ggacctgggc
    20941 ttataaccat atcggctatg accgctacca cgaaggtgtg gctatcttgt ctaaaacacc
    21001 tattgaagcc agagaaattt tggtttcaga tgtggatgat ccaacagact atcatactcg
    21061 ccgtgttgcc ctagctgaaa ctgtagtcga tggcaaggag ctagcagttg ccagtgttca
    21121 tctctcttgg tgggataaag gtttccaaga agaatgggca cgatttgagg ctgtcttgaa
    21181 aaaattgaac aagccacttt tactagctgg agattttaac aacccagcag gtcaggaagg
    21241 ttaccaagct attttagcta gtccattagg cttacaagac gcatttgaag ttgctcaaga
    21301 gaaaagtggt agctatactg ttccgcctga aattgatggc tggaaaggga acactgaacc
    21361 ccttcgaatc gattatgtct ttactaccaa agagttagcg gtggaaaatt tacatgtcgt
    21421 atttgatggt aacaagagtc cacaagtgag tgatcactat ggcttgaatg ctatattaaa
    21481 ctggaaataa taactgaaaa gaggttggaa ctataaaatt ccagcctttt cttactagag
    21541 aagctactgg aaatagccta aataagtgag actactgtaa tggaataaaa tatggtataa
    21601 ttgataagat agatagaatc gaggatgtta tgtcatttac gaaatttcaa tttaaaaact
    21661 atattagaga agccttgaag gagttaaaat ttacaactcc aacagaggtg caagacaagt
    21721 tgattcctat tgttttggca ggtcgtgacc tagtaggaga atcaaaaaca ggttcaggta
    21781 agactcatac tttcttgtta ccgattttcc agcaattaga tgaagctagc gatagtgtac
    21841 aagcagtgat tactgcaccg agtcgtgagt tggctactca aatttaccaa gtagcgcgtc
    21901 agatttcagc tcactcagat gtcgaagttc gtgtggttaa ttatgtgggt ggtacggata
    21961 aggctcgcca gattgagaaa ttggaaagca atcagcctca tattgttatt ggaacaccag
    22021 gccgtatcta cgacttggtt aaatctggtg atttagctat tcataaagcc aagacatttg
    22081 ttgttgatga agcagatatg accttggata tgggattctt ggaaactgtt gataagattg
    22141 ctggcagtct tccaaaagac ttgcaattca tggtcttctc agcgactatc ccacaaaaac
    22201 tgcaaccatt cttgaaaaaa tacttatcaa atcctgttat ggagaaaatt aagaccaaaa
    22261 cggttatttc tgacaccatt gataattggt tgatttcgac caagggacat gataagaatg
    22321 ctcaaattta ccagttgact cagttgatgc agccgtattt ggcaatgatt tttgttaaca
    22381 ctaaaacgcg tgctgatgaa ttgcattcat atctgactgc tcaaggcttg aaggttgcaa
    22441 aaatccatgg agatattgcc cctcgtgaac gcaagcgaat catgaatcag gtgcaaaatc
    22501 tggattttga gtatattgtc gcaacagatt tagcagcgcg tgggattgac attgaaggtg
    22561 tcagccatgt catcaatgat gccattccgc aagacttatc tttttttgtt catcgtgttg
    22621 gtcgtactgg acgaaatggc ctaccaggta cagctattac cctttatcag ccaagtgatg
    22681 actcggatat ccgtgagttg gagaaattgg gaatcaagtt tagtcctaag atggtcaaag
    22741 acggggaatt tcaagatacc tatgaccgtg atcgtcgtgc caaccgtgag aaaaaacaag
    22801 ataaacttga tatcgaaatg attggtttgg ttaaaaagaa aaagaaaaaa gtcaaaccgg
    22861 gttataagaa gaaaattcaa tgggcggttg atgaaaagcg ccgtaaaacc aagcgtgctg
    22921 aaaatcgcgc tcgcggtcgt gcagagcgta aagctaaacg ccaaacattt taatagaaat
    22981 tgttggagta ttgagctcca actttttgct ttatgagaac gaactatcta aaccgaaaca
    23041 ctacattaaa gactgcaaat tgcgattaaa aatggtataa tgataaagtt atatagtccc
    23101 gataagatgg taggtattta ttacgaagag ttttcctatc agtactttgt aactctataa
    23161 caatattttt taagggggga catttttatg tcagaacgta aattattcac gtctgaatct
    23221 gtatctgagg ggcatccgga taagattgca gaccaaattt cagatgcgat tttggatgct
    23281 attttagcaa aggatccaga ggcgcacgtt gctgctgaaa cagctgtata tactggttct
    23341 gtccacgttt ttggtgaaat ttctacaaat gcctatgtgg atattaaccg tgtggttcgt
    23401 gataccattg cagagattgg ttataccaat acagaatatg gattttctgc tgagacggtg
    23461 ggagtacacc catctttggt ggaacaatct cctgacatcg ctcaaggtgt taacgaagcc
    23521 ttggaggttc gtggaaatgc tgatcaagat ccactggact tgattggagc aggtgaccaa
    23581 gggctcatgt ttggatttgc agtagatgaa acagaagagc tcatgccatt gccaattgca
    23641 ctcagtcata aattggttcg tcgtctggca gaacttcgta agtctggaga aattagctat
    23701 ctccgtccag atgcaaaatc acaagttaca gttgagtacg atgaaaatga ccgtccggta
    23761 cgtgtagata cagtcgttat ttctactcag catgatccag aggccactaa tgaacaaatc
    23821 catcaagatg tgattaacaa ggtcatcaaa gaagttattc catcttctta tcttgatgat
    23881 aagacaaaat tctttatcaa tccgacaggt cgttttgtaa tcggtggtcc tcaaggggac
    23941 tcaggtttga ctggtcgtaa gattattgta gatacttatg gtggctactc tcgtcatggt
    24001 ggtggtgcct tctctggtaa agatgcgact aaggtggatc gttcagcctc ttatgcggct
    24061 cgctatattg ccaagaatat cgttgcagca ggccttgcta agaaggcaga agtgcagttg
    24121 gcctatgcta tcggtgttgc gcaacctgtt tctgttcgta tcgatacttt cggtacagga
    24181 acagtagctg aaagtcaact tgaaaaagcg gctcgtcaaa tctttgacct tcgccctgca
    24241 gggattatcc aaatgctgga cctcaagcgt ccaatttacc gtcaaacatc ggcttacggt
    24301 cacatgggac gtacagatat tgatcttcca tgggaacgtt tggataaggt agatgctttg
    24361 aaagaagcag taaaataaga ttttaagagg ggaacgtcct ctctttttta tagtttttaa
    24421 ctatactggg atactgttct gaaaatccat tttgcgaaag tagagattta catgtatagt
    24481 agattgaaac tagaatagta caccttaact tctaaaacat tgttagcaat caatttgact
    24541 gtcctgatcg atttctcctg ttcttgtttc attttactat atttctttaa aaatgataaa
    24601 ggttgagatt tctcctcgta atagataatc atggggatat ttcaatccaa agttttattc
    24661 gttatcactt gactattgca aggttttcta gagcaacaga gtcatggaat ggactcatgg
    24721 ttgagatttc tccttgttgc ttggacttca ttcaaaagtc tgttacccaa gccttgttca
    24781 aacttccaat acactagctg ttttcatagc atgacttctg tactagactt tcttttccga
    24841 ataaatagat agaaccacag aatctagtaa acctagaatt aaaattatgg tataatatta
    24901 gcaataaaag aaatctggag gattagaatc atggtatcaa cgaaaacaca aattgctggt
    24961 tttgagtttg acaattgctt gatgaatgca gcaggtgtgg cttgtatgac gatagaggag
    25021 ttagaagagg tcaaaaactc agcggcagga acctttgtta ctaagacagc gaccttggac
    25081 ttccgtcagg ggaatcctga gccacgctac caagatgttc cacttggttc catcaactct
    25141 atgggcttgc caaataatgg cttagactat tatttggatt atcttttaga tttgcaggaa
    25201 aaagagtcga accgaacttt cttcttatct ctggtcggca tgtctccaga ggaaacccat
    25261 actattttga aaaaagttca agagagtgat tttcgtggtc tgactgagct aaatctttcc
    25321 tgtccaaatg ttccaggtaa acctcagatt gcctatgatt ttgagacaac agaccggatt
    25381 ttggcagaag tgtttgctta cttcaccaaa cctcttggaa ttaaattgcc accttatttt
    25441 gatattgttc actttgatca agcggcagct attttcaaca aatatccgct caagtttgtc
    25501 aactgcgtta actctatcgg aaacggcctc tatatagaag acgaatctgt cgttattcgg
    25561 cctaagaatg gttttggtgg aattggtgga gaatacatca aaccgactgc tctagccaat
    25621 gttcacgcct tttatcaacg tttaaatcct caaatccaaa ttatcggaac aggtggcgtt
    25681 ctgactggtc gagatgcctt tgaacacatc ctctgtggag caagtatggt gcaggtggga
    25741 acgacccttc acaaagaagg cgtcagtgct tttgaccgca ttaccaatga actgaaagca
    25801 atcatggtgg aaaaaggcta cgagagctta gaagatttcc gtgggaaatt gcgctatatt
    25861 gactaaatta aatcgaaaaa tctgaagaaa ggagagacga tgctagccat tgaagaaagt
    25921 cagaagttga ctttatcaaa tttaccgagc ctgagcctat ttacagggac agatcagggt
    25981 cagtttgaag tgatgaagag tcaagtgttg aaacagattg ggtatgattc tgctgacctc
    26041 aactttgcct actttgatat gaaagaagta gtttacaagg atgtggaact ggagttggtc
    26101 agccttcctt tctttgcgga tgagaaaatc gtgatattag attattttat ggatatcacg
    26161 actgctaaga aacgcttttt gacagatgat gagcttaagt catttgagga ataccttgac
    26221 aatccttctc caacaaccaa gttgataatc tttgcagaag gaaagctgga tagcaaaaga
    26281 cggttagtca aattacttaa gcgtgatgcc aaggccttcg atgcagtaga agtaaaagaa
    26341 caagaattgc gccagtattt ccaaaagtgg agtcagaaac aaggtctgca gtttaccaat
    26401 cattcttttg aaaatctcct catcaagtcg gggtttcaat ttagcgaaat ccagaaaaat
    26461 cttctctttt tacagtccta taaggcggat tctgttatcg aggaagagga tattgttaac
    26521 gcaattccca agaccttgca ggacaatatt tttgatttaa ctcagtttat tctgactaaa
    26581 aagatggatc aggcgcgcga tttggtgaga gacttgacct tgcaagggga agatgaaatc
    26641 aaactaattg cagtcatgct gggacaattt cggactttta ctcaggtgaa gattttggcg
    26701 gagtctggcc aaacagaatc gcagattgca agtagtttag gtagttatct gggacgtaac
    26761 ccaaatcctt atcaaatcaa gtttgcatta agagattcga gaggactttc tttgagcttt
    26821 ctgaagcaag ctatttccta tttgattgag acagactatc agattaagac aggtctttat
    26881 gaaaaaggtt tcctttttga aaaggcactc ttacagattg ctagtcaggt caattgacat
    26941 ttgttgaaac tacaacccgc gtcaggatta tcttgtcgtg ggtttcttgc tgatttgctt
    27001 atctataagc aaaaaacttg aaaaaagtga atgtttgcgt tatcattttc atatagaaag
    27061 aaaaagaggt attacagatg gctattatct taccagaact tccatatgca tacgacgctt
    27121 tggaaccata catcgatgcg gaaacaatgc atttgcacca tgacaaacac catcaaactt
    27181 atgtcaacaa tgccaatgca gctttagaaa aacaccctga aatcggtgaa gaccttgaag
    27241 ccttgcttgc tgatgtagaa tctatcccag ctgatatccg tcaagcactt atcaacaatg
    27301 gtggcggaca cttgaaccac gctcttttct gggaattgat gactcccgag aaaacagctc
    27361 cttcagcaga actggcagca gcaatcgatg caacatttgg ttcatttgaa gaattccaag
    27421 cagccttcac tgcagcagca acaactcgtt ttggttcagg ttgggcatgg ttggttgtca
    27481 acaaagaagg gaaacttgaa gtgacttcaa cagcaaacca agacacacca atctcagaag
    27541 gtaaaaaacc aatcttgggc ttggacgttt gggaacatgc ttactacgtg aaataccgca
    27601 acgtgcgtcc tgactacatc aaagctttct tttcagtaat caactggaat aaagtagatg
    27661 aattgtacgc agctgctaaa taatgatagt tggagggaag aattgttctt ctctttttta
    27721 ggttataaga ttctggtctg acaaaatcgt tagacttttt tcatttttta tgagaaacgt
    27781 gctaactgct agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct
    27841 atgcgaaaag aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc
    27901 catttacacg gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc
    27961 aaggatgcct ttgatgtgac gacttttaat cagcgttttt cagaagtctt aaccaagttt
    28021 gattatatcg tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat
    28081 gaccgaacag aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat
    28141 tgtagttatg gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt
    28201 gacaagaaaa tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact
    28261 caaactaaac gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccagcat
    28321 tctcagaaag aggacaagga acaacgtcat tttgtcattc gtcagaagtg agtagagaat
    28381 aaggagaaga aatgaaaccc tcaattcata gtttggtaca tcaaaccatg caggaatggg
    28441 tattggagca gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta
    28501 aacgtgtgca gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca
    28561 atgaccagtt tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta
    28621 cagtcaaata tctctttgaa ttgcctgacg gtatgttaat tgagactgta ctcatgcgtc
    28681 aacactatgg tttatctgtc tgtgtgacca ctcaggtcgg ctgtaatatc ggttgtacct
    28741 tctgtgcctc tggtttgatt aagaagcagc gtgacctcaa taacggtgaa atcgtagcgc
    28801 aaatcatgct ggttcagaaa tactttgctg atgaaggggt tcaggtcaat cttgccgtgt
    28861 ctcttcacgc acccaacaat gaacttcgtt caagtatcat gaagattaac cgtgcctttc
    28921 cgattgaaaa actctttgct gctattgaat actacattga gacaacaaac cgtcgtgtaa
    28981 cctttgaata tatcatgctt aatgaagtca acgacggtgt agaacaagcc ttggaattgg
    29041 ctgaattgct caagaacatc aagaaattgt cttatgtaaa cttgattcct tataacccag
    29101 ttagtgagca tgaccaatat agccgtagtc ccaaagagcg cgtgttggct ttctatgata
    29161 cgcttaagaa aaaaggggtc aactgtgtgg ttcgtcaaga gcatggtaca gatattgacg
    29221 cagcttgtgg acaattgcgc tctaacacaa tgaaacgtga ccgccagaag gcagtcgcag
    29281 cggtaaatcc ttaatgatga agaagactta taatcatatt ttggtctggg gagtcatttt
    29341 ctatagcatt tgcattgtct gtttttgctt tactcctcaa gaacaatcta ccgtgggagt
    29401 gggaactcca ggtattcagc atcttggacg cctggttttt cttttgactc ctttcaattc
    29461 tctctggaaa ctgggcgaag tgagtgacat tggacaatta tgttggattt ttttacaaaa
    29521 tgtcctcaat gtcttcttgt tttttcctct gattttccaa ctcctttatc tatttccaaa
    29581 tttgcggaaa acaaaaaagg tccttctttt tagttttctt gtgagtcttg gaatcgagtg
    29641 tacgcaatta atcttggact ttttctttga tttcaatcgc gtctttgaga ttgatgattt
    29701 gtggaccaat actttgggtg gctatctggc ttggctcctt tataaacgat tacataaaaa
    29761 caagataagg aattaaaatg agtattttag aagttaaaaa tctgagtcac ggttttggtg
    29821 accgtgcaat ttttgaagat gtgtccttcc gtctcctcaa gggagaacat atcggcctgg
    29881 tcggtgccaa tggtgaagga aaatcaacct ttatgagtat cgtgactggt aaaatgctgc
    29941 cagatgaagg aaaggttgag tggtccaaat atgtgacggc tggttacttg gatcagcact
    30001 ctgtccttgc tgaaagacag tcggtgcgtg atgttctccg tacggctttt gatgagcttt
    30061 tcaaagctga agctcgtatc aatgacctct atatgaaaat ggctgaagac ggcgcggatg
    30121 ttgatgctct catggaagaa gtaggagaac ttcaagaccg tctggagagt cgtgatttct
    30181 ataccttgga tgctaagatt gacgaagtag cgcgtgctct tggtgttatg gactttggca
    30241 tggatacgga tgtaacttct ttgtcaggtg ggcaaagaac caaggtgctt ttggcaaaac
    30301 ttctccttga aaagcctgat atcttgctgt tggacgagcc gaccaactac ttggatgctg
    30361 agcatattga ttggctcaag cgctatctcc aaaactatga gaatgccttt gttctcattt
    30421 cgcacgatat tccattcctc aatgacgtta ttaatattgt ctatcatgtg gaaaatcaac
    30481 agctgacgcg ttactctggt gactactacc agttccaaga agtttatgct atgaagaaat
    30541 ctcagctaga ggcagcctac gaacgccagc agaaagagat tgcagacctc aaggactttg
    30601 tggctcgtaa taaagcccgt gttgcaactc gtaatatggc tatgtctcgt caaaagaaat
    30661 tggataagat ggatattatc gaactccaaa gtgagaaacc aaaaccatcc tttgatttca
    30721 aaccagctcg tacaccaggg cgctttatct tccaagccaa gaacttgcaa attggttacg
    30781 accgtcctct tactaagcct ttaaatctta ccttcgaacg caatcaaaag gttgcgatta
    30841 ttggtgctaa tggtattgga aaaacaactc tcttgaagag tctcttgggc attatctcgc
    30901 caatcgctgg ggaagtggag cgtggagatt atttagaact tggttatttt gagcaggaag
    30961 tagaaggtgg taatcgccaa actcctcttg aagctgtctg gaatgccttt cctgccctta
    31021 atcaagcaga agtccgtgca gcccttgccc gttgtggttt gacaaccaaa catattgaaa
    31081 gccagattca agtattatca gggggagagc aagccaaggt tcgtttctgt ctcttgatga
    31141 atcgtgaaaa caacgtttta gtgctggacg agccgaccaa ccatttggat gtggatgcaa
    31201 aggatgagct caaacgcgct ctcaaagaat acaggggatc tatccttatg gtctgccacg
    31261 agccagactt ttatgaaggt tggatagacc aaatatggga ttttaataat ttaacttaag
    31321 aatgagagta aaagaaatac ggctctttgt caactgtagt gggttgaagt cagctaagct
    31381 cgagaaagga caaaattcgt cctttctttt ttgatgttca gagcgataaa aatccttttt
    31441 ttgaagtttt caaagttccg aaatccaaag gcgtttcgtt tgataagttt gatgagatta
    31501 ttggtcgctt ccaattttgc gttggaataa ggtaattgaa gagcgttgac gattttttct
    31561 ttgttcttta gaaaggtttt aaagacagtc tgaaaaatag gatgaacctg cttcagattg
    31621 tcctcaatga gtccgaaaaa tttctcaggg tctttgttct gaaagtgaaa aagtaagagt
    31681 tgatagatct gatagtggtg tttcaagtct tctgaatagc ttaaaatctt gtcaagaatt
    31741 tctttatttg ttaagtgcat gcgaaaagta gggcgataaa aacgtttatc gctcaattta
    31801 cgactatcct gttggatgag tttccagtaa cgtttgatag ccttgtattc atgagatttt
    31861 cgttcaaact gatt
//
LOCUS       DAVLWA010000017        27792 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLWA010000017 DAVLWA010000000
VERSION     DAVLWA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.017"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(114..749)
                     /locus_tag="VYN80_000748"
     CDS             complement(114..749)
                     /locus_tag="VYN80_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1540695.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(751..1578)
                     /locus_tag="VYN80_000749"
     CDS             complement(751..1578)
                     /locus_tag="VYN80_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1540696.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1600..1935)
                     /locus_tag="VYN80_000750"
     CDS             complement(1600..1935)
                     /locus_tag="VYN80_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV1540697.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(1936..2652)
                     /locus_tag="VYN80_000751"
     CDS             complement(1936..2652)
                     /locus_tag="VYN80_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1540698.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRAQAPLVIKEVNAIKVALLAYSYGFNGIEQYISQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            3165..3776
                     /locus_tag="VYN80_000752"
     CDS             3165..3776
                     /locus_tag="VYN80_000752"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1540699.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGKKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3821..4549
                     /gene="rlmB"
                     /locus_tag="VYN80_000753"
     CDS             3821..4549
                     /gene="rlmB"
                     /locus_tag="VYN80_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1540700.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4588..5499)
                     /gene="trxB"
                     /locus_tag="VYN80_000754"
     CDS             complement(4588..5499)
                     /gene="trxB"
                     /locus_tag="VYN80_000754"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1540701.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTNNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5565..5789)
                     /locus_tag="VYN80_000755"
     CDS             complement(5565..5789)
                     /locus_tag="VYN80_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1540702.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5867..6610)
                     /locus_tag="VYN80_000756"
     CDS             complement(5867..6610)
                     /locus_tag="VYN80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1540703.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6610..7410)
                     /locus_tag="VYN80_000757"
     CDS             complement(6610..7410)
                     /locus_tag="VYN80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1540704.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7568..7924)
                     /locus_tag="VYN80_000758"
     CDS             complement(7568..7924)
                     /locus_tag="VYN80_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1540705.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7937..8449)
                     /locus_tag="VYN80_000759"
     CDS             complement(7937..8449)
                     /locus_tag="VYN80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1540706.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(8449..8943)
                     /locus_tag="VYN80_000760"
     CDS             complement(8449..8943)
                     /locus_tag="VYN80_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540707.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            9078..9524
                     /locus_tag="VYN80_000761"
     CDS             9078..9524
                     /locus_tag="VYN80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1540708.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9521..10168
                     /locus_tag="VYN80_000762"
     CDS             9521..10168
                     /locus_tag="VYN80_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1540709.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10189..10770)
                     /gene="pdxT"
                     /locus_tag="VYN80_000763"
     CDS             complement(10189..10770)
                     /gene="pdxT"
                     /locus_tag="VYN80_000763"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1540710.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10771..11646)
                     /gene="pdxS"
                     /locus_tag="VYN80_000764"
     CDS             complement(10771..11646)
                     /gene="pdxS"
                     /locus_tag="VYN80_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1540711.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11798..13177)
                     /locus_tag="VYN80_000765"
     CDS             complement(11798..13177)
                     /locus_tag="VYN80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1540712.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13471..14394
                     /locus_tag="VYN80_000766"
     CDS             13471..14394
                     /locus_tag="VYN80_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1540713.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14454..15059
                     /locus_tag="VYN80_000767"
     CDS             14454..15059
                     /locus_tag="VYN80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1540714.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            15078..16322
                     /locus_tag="VYN80_000768"
     CDS             15078..16322
                     /locus_tag="VYN80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1540715.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16545..16802)
                     /locus_tag="VYN80_000769"
     CDS             complement(16545..16802)
                     /locus_tag="VYN80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1540716.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16844..18880)
                     /locus_tag="VYN80_000770"
     CDS             complement(16844..18880)
                     /locus_tag="VYN80_000770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1540717.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(19092..20009)
                     /gene="glyQ"
                     /locus_tag="VYN80_000771"
     CDS             complement(19092..20009)
                     /gene="glyQ"
                     /locus_tag="VYN80_000771"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1540718.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(20202..20963)
                     /locus_tag="VYN80_000772"
     CDS             complement(20202..20963)
                     /locus_tag="VYN80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1540719.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(21236..22078)
                     /locus_tag="VYN80_000773"
     CDS             complement(21236..22078)
                     /locus_tag="VYN80_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1540720.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(22192..23583)
                     /locus_tag="VYN80_000774"
     CDS             complement(22192..23583)
                     /locus_tag="VYN80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1540721.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTPFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(23821..24285)
                     /locus_tag="VYN80_000775"
                     /pseudo
     CDS             complement(23821..24285)
                     /locus_tag="VYN80_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            24439..25416
                     /locus_tag="VYN80_000776"
     CDS             24439..25416
                     /locus_tag="VYN80_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1540722.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            25413..26495
                     /locus_tag="VYN80_000777"
     CDS             25413..26495
                     /locus_tag="VYN80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1540723.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(26559..27589)
                     /locus_tag="VYN80_000778"
                     /pseudo
     CDS             complement(26559..27589)
                     /locus_tag="VYN80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         8132 a         6240 c         4998 g         8422 t
ORIGIN      
        1 aataatccgt acaatctgat ttacattcat aaattcctcc gtcacttttt attttaaagg
       61 attttagaat tttttacaag atattatgct caaatttaac attatttagc tatttagtaa
      121 agatagtctt tcaaggcttt tgacaaagcc tgataaccag caatactgag gtgcagtcca
      181 tcagttgtat attctttttt gagttggcct gcttggtctg tcaaacaatc aaatactggc
      241 acaaattcca cctgcatata ggcagatgca agctcttgat aggcttgatt ccagttctga
      301 attttttcat tcgagcggat atagactgcc tgctggtact cctctctctc attgacaggc
      361 aaaatggaaa gcaatttaat ctctgtcaat ggataatcgc gagcaacgga ttgaatgata
      421 gcttcgagat tattgagagc ctcattcaca ggaacatcct ttccgatatc atttgtccca
      481 atcagaagaa aaattttatc tactgctcca ccatatagat gagcatcaag gttctctagt
      541 aacagtcctg tctgatagcc acgaattcct cgattgacaa tcgtctttga agtcccaaat
      601 agctcctgta gaggataata ctcgacaatg gaatccccaa taaaaagaat gtttggttct
      661 acaacagaaa tgtgatttag gtgacgatac ttagtttgaa ttttttcttg ttcctttagg
      721 agccaatttt ctaataactg tactgccact tcattctcct ttttctaacc agttttccaa
      781 gacttgataa actcctcctt ggctgttggc tggtgctaga gcagtcgcca cagctttggc
      841 tttctcatca gcattttcca tcgcataggc aattccagcc atttcaagca tttcaacatc
      901 attttcacta tcaccaaaag ccatgatttc ttgggatttc aagtcccagc gcttgagtaa
      961 ttcctccaag ccccatgctt tatgaatccc agcttggagg atatcaatgc aaccatagcc
     1021 actggataca gctcggacac ggccatcaaa gagagcattg atttcttcca aaaccgaact
     1081 caaacgttcc tcaccaacaa ccatgctcat cttgagcaca ccaccaaaga ggtcagatgt
     1141 taattcatcc acaaattgca tccgttggta gaatttttca atcatttctg gagtcataaa
     1201 actttcaaga tctgtaaaaa tcgtaccttc cttgacaaaa tcacccttca tccccgttac
     1261 aacaaactgg tcctgacacg ctcgaccctt gaaatgagtc aaagccttgt tgacaatggc
     1321 gtcatcccat gtctgagcct gaatcaattc attgttttca aaaatacgag cgccattagc
     1381 aacaaccaga accactcgat ccaccaaggg actcagtagt tgtctcatgc ggtgaatttc
     1441 attgcccgtc gcaatgacaa aacgaatgcc cctttgatcc aactgatcta aaatcttttc
     1501 caatcgtggg agatcaagct gacctctagc atccagcaag gtcccatcca tatctgtcgc
     1561 aattacctta atcatcattt actttcctct ggattcaagc taataccact tcaatcccac
     1621 atgttcattc atttctttat aggctgtatc aattcgttcc ttagtcgctt catctaactg
     1681 gtcacgatga ctgccatcct ctataaaatc ttccaaaata taagtttggt aatgatacaa
     1741 gggatatcct tctggtgaaa aggttccctt tggtgttcga ttgacccaag taggatgagc
     1801 tttagctgtt ccgatagttg tttttccatc cttcttcttg atggtgacat ccatgagaac
     1861 accacgttca gtccacttag cattctcttc atctcccata gattcaattc gttgattgga
     1921 aatgaagttc cccatttaat agacaatgag cttcttatct ccatcttttt ccaccgtttc
     1981 agatggttca acaacgtgag gatgccctcc aaagataata tccgctcccc aatcaatcat
     2041 cttgtgataa agagcttttt gttcttcagt tggttccaat cgatactcaa cacccatctg
     2101 aggcatgata atggtgatat ctgcttcctt ctctgcccgt tcaacttcag cctttatctt
     2161 atcttcgttt aaatctgaaa gataacgatt atagtcttcc tgagaaatat actgctcaat
     2221 tccattgaaa ccataggaat aggctaacaa tgcaaccttg atagcattca cttccttaat
     2281 gaccagcgga gcctgagcac gtggttcgtg cgtataaact ccgattggag tgattccagc
     2341 tttctcaata atatcggcca ttgaaataac tccctcaatt tgcgaatcca aaatatgatt
     2401 atgagctaaa tccagcacat gataacctgc ctccttaata gcatccataa cttcaacagg
     2461 agcattaaag agaagataac ccgctaaata atgatcctta ttaatggttc cttcaaaatc
     2521 accaatagct aaatctgctt gcttgagcca aggagtcaca tactcaaaat tttcatgaaa
     2581 gtcataggta ccgtcttctt ttttagctga aaagaaaagt ccatcgtggt agagcaaatc
     2641 cccgtgggcc ataattctgg cagttttttc ctcttcctac tcctgagact tttgcttagt
     2701 cccctcttga gaaatagtat ctcgtttttg actagtcaag ggattccttg gaagctttca
     2761 aacaacaaga ccaagcccat tgataaacca actgctagca agagtatcgc cacaaatccc
     2821 ttattgctcc acttgcgata actcctaaaa agtttaccaa gcccttcata aaacgaaaag
     2881 ctaaaccacc ctgatttcga ttttgtcttc tttgtatctt cgttctccct actttcttat
     2941 gcaagccttt tctttttatt atatcacaga taagtatttc tttcacaatt gaattgaact
     3001 tcctatctat tttctataaa tcctaaatgc cataatgctt tcaattcttg tcattttgtg
     3061 atatcatgta gaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc
     3121 tttggcagta gctaactgca ctaaacataa tataaggaga gaaaatgtct gcaatagaac
     3181 gtattacaaa agctgctcac ttaattgata tgaacgatat tatccgtgaa gggaatccta
     3241 ctctacgcac ggttgctgag gaagtcactt tccccctatc tgaccaggaa atcatcctag
     3301 gcaaaaagat gatgcaattc cttaaacatt cccaagatcc tgtcatggct gaaaaaatgg
     3361 gactccgcgg tggtgttgga ctggctgctc cccagttaga tatctcaaaa cgcattatcg
     3421 ctgttttggt acctaatatt gttgaagaag gcgaaactcc acaggaagcc tacgatttgg
     3481 aagccattat gtacaatcca aaaatcgtct ctcactctgt tcaagatgct gctcttggcg
     3541 aaggagaagg ttgcctgtct gttgaccgta acgtgcctgg ctatgttgtt cgccatgccc
     3601 gcgttactgt tgactacttt gacaaagatg gagaaaaaca ccgtatcaaa ctcaaaggct
     3661 acaactccat tgttgttcag catgaaattg accacattaa cggtatcatg ttttacgatc
     3721 gcatcaatga aaaagaccca tttgcagtta aagatggttt actgattctt gaataaagaa
     3781 aatcccgttg caagacgggg ttttgtgtta taatagaggc atgaaaacaa atgatattgt
     3841 ctatggtgtc cacgctgtta ccgaagccct ccttgcaaat acaggaaaca aactctacct
     3901 ccaagaagat ctccgaggta agaatgttga gaaagtcaag gaactagcta cagaaaagaa
     3961 ggtgtccatt tcttggacat caaaaaaatc tctctctgag atgactgaag gtgctgttca
     4021 tcaaggtttt gttctacgag tgtctgaatt tgcctatagc gagctagatt acatccttgc
     4081 aaaaacacgc caagaagaaa atccacttct attgattcta gatggtctaa ccgatcccca
     4141 taatctgggt tctatcttgc gaacagccga tgcgaccaat gtttcaggtg tcatcattcc
     4201 caagcaccgt actgtcggag taactcctgt cgttgccaaa acagccacag gtgctattga
     4261 acacgttcca attgcccgag tgaccaacct cagtcaaacc ttagataaac ttaaggatga
     4321 aggtttctgg acctttggaa cggatatgaa cggtactcct tgccacaagt ggaatacaaa
     4381 agggaaaatc gccctcatca ttggaaatga aggaaaaggt atctctagca acatcaaaaa
     4441 acaggtcgat gaaatgatta ccattccgat gaatggacat gttcaaagcc ttaatgccag
     4501 tgttgctgcg gccattctca tgtacgaagt tttccgaaat agactataaa aaagtttcca
     4561 gtcatctgat tggaaacttt tttatgatta actatgttct gtaatgaatt tataggcttc
     4621 ttgaccagcg atagctccat ctccaaccgc tgttgttact tggcgaaggt ctttcaagcg
     4681 aacatctcca actgcaaaga taccgtcgac tgcagttttc atgtggttat ttgtcacaat
     4741 ccatcctgcc tgatcttgga tattcaattc tttaacaaaa tcgctaagag ggtccaaacc
     4801 aacatagata aagacaccac cgaaggcttg ttctgtcact tgacctgttt tcacattttc
     4861 aaatacgact gattctactc ggttttcacc cttgatttcc cttactacag aatcccagat
     4921 aaagctgatt ttttcattcg caaaggcgcg atcttgtaaa accttttggg cacgaagttg
     4981 gtcacgacgg tgaacaatgg taacagtctt agcaaaacga gtcaagaaga gggcttcttc
     5041 aacagctgaa tctccaccac caactaccaa taaatcttgg tcacggaaga aagcaccatc
     5101 acacacagca cagtaagaaa caccacgact gttcagttct tcttctccag gcactcccaa
     5161 aggacggtgt ttagaaccag ttgctacgat aactgtacgt gtttcatatg tttggtcatc
     5221 agtcatcact ttcttaaaat caccatggtc ttcgacattt tcaacataac cataaatgtg
     5281 ctcaacacca agattttcaa gtggttcaaa catcttttca gccaattcag gtccactaat
     5341 attagcgtat cctgggtaat tttcgatatc agatgtatta ttcatctgac cacctggcag
     5401 accaccttca atcaaagcta cttttagatt gcttcgagca gcatacaagg ccgcagtcat
     5461 ccctgcaggt ccagcaccga taataatagt atcgtacata tagattcctt ctttcttgtt
     5521 gtaactatct ttattctaac tctttcttgt caatcacgca aggattatgc cttgaaaaca
     5581 agaattaaac tcataaccgc aaaggataat actgggacaa ccaagactcc aatcataatc
     5641 atatcataga gatgggcttg gcgagccctg gctgtcttat caactcccga catggctctc
     5701 agaccaatcc aaatccctaa aaaaatcaga acaaggatgg tggtcaagat caaactctcg
     5761 aaatataaag aaaatagttg cagtagcatg atttctctca tttctatctt ttttaaagag
     5821 taaactcagc tagtccaact aactgagttt tcctttatct attatatcaa atataagtcc
     5881 gtttgtaact agcgaagaat tcttttgtcc gctcttcttt aggggtgtgg ataatctcat
     5941 ccggagttcc agactcgatg attttcccct tatctaagaa gagaatttta tccgcaactt
     6001 gggctacaaa ggacatgtca tgactgacca aaatcatggt ctgacctgac ttagcagcat
     6061 ctgcaataga cttttctact tcaccgacca attctgggtc aagggctgaa gttggttcgt
     6121 ctaagagcaa aacatctggt ttcatagcaa gcgcacgcgc tagggcaacc cgttgcttct
     6181 gtccacctga taaatggcga ggataatggt tttcacggtc cgaaagccca accttagcca
     6241 actcttcctt ggcaatctta gtcgcttctt ggtcagataa tttcttgaca acaaccaagc
     6301 cttctttcac attatcaagt gctgttcggc gttcaaataa attaaactgt tggaaaacca
     6361 tagacaactt acggcgtagg gcaaggattt cttcttgagt gattttagaa aaatcaactg
     6421 aaaaaccatc aatctgaata gagccactgt caggtgtttc aagataattg agactgcgaa
     6481 gaaaggttga ttttccagct cctgaagaac caatcaaggc tacgacttct cctttttgaa
     6541 tatccaagtt cagatgatcc aagacagtct gtcctgaaaa ggatttgctt aaattcgaaa
     6601 tcttaatcat taacgaaggt ctcctttcac atctgtttgc actgtatcag gtgcagaaat
     6661 agccattttt ctctcgatga aacgaccgag gctttcaatt ccgatattga ctacccaata
     6721 aacaagggca acggagatga agcgttcaaa atagcgataa tcagctccac ctagaatctg
     6781 agcttgggca aagacttcca caacacccgc actaaaagct agagatgttc ccttggtcaa
     6841 accgatgagg gaattaatca aggttggagt agctaccacc gctgcattag gaataatcac
     6901 tcgtcgataa acttgcgctc gggtcatacc cagactgcgt gccgcctcaa tctcaccagg
     6961 attaactgag agaatggctg cacgaatggt ttcactagca taagctgcct cattaaaggc
     7021 aaaagcgaca atcgcaaaag ctgcagctgg aatcgcattg atattgagac cagttcccca
     7081 ttgctgattg agggctttca aagccaaagg gattccgtag taggtcaaca tgagttgcac
     7141 caaaatcggt gtccctttta agaaactaac aaagaaggcc tgcaagggat ataaaatctt
     7201 gacacgattg atcttcacaa tggcaaaaag aagcgccaaa accaagccaa aaagggcacc
     7261 accaattgtc aacataattg ttgttggaag ttgttggaca attctaggga ttccatcaaa
     7321 gaccgaacgt aggctaaaca gcttgccatc cggaatcaat tgcatcaagt tttggtacca
     7381 atctgatgct aaaatcgttg taacattcat aaaaacatcc tcttctgata atgattcatt
     7441 ctaccatgct tctgccgtca atgaaattat ttgctacggt cagatacaga ctttgtctac
     7501 ctactaggtt atcatacttt gaaacaggga agttatatat tttatctatc aaagagatag
     7561 ataaaaacta tttcagtccc agttcttcat aagattttcg ataaccgatt tgcttaacag
     7621 ttccattttc tactaaaatg ggccgtttta acaacatacc gtcacttgct agcaactcag
     7681 ccgcttcttg gtttgacaaa cttcctacct tatcttttag ccctaattca cggtatttga
     7741 taccactggt gttgaaaaat tgcttcaatt caaatcctga ggtttctagc caattcaaaa
     7801 tgacttcttg gctaggtgtt tcttccacga tatggacggc tttatagtcc acacctaatt
     7861 gatttaattc ttgttttgct tttttacaag ttgaacattt ggggtattcg ataaattcta
     7921 acatgtgctt ctccttctat ttgtttatat ctttaaaatc tacgccttca tgctccaaga
     7981 gccaagcttt cttttccact cctgcagcat agcctgtcag acgcttgcct gctcccaaca
     8041 cacgatgaca aggtactagg atagaccaag gattgcgtcc cactgctcca ccaattgctt
     8101 gagcagaagc cacttgcagg tcttgagcaa tttgtccata ggtcactgtc tgaccataag
     8161 gaatgccctg taaatagtcc caaactctct tttcaaaatt cgttccgatt ggcgccaaga
     8221 gcaagttgga taaatcctga ggcttgcctt taaagtaatc atctaagcaa gcaataactg
     8281 ggtctaaaat aggatgacta acaacttctt ctatcgtttc atctcctagt cccctctcaa
     8341 aatgcttctg ctcctgaacc caaattccat acaaataatg gtcatcagct acaagtgata
     8401 gaataccaat tggtgaggag tagagtattt ttacatactt cttttgcatt atttctttaa
     8461 tttttgatag gccaagcgtt cgccatctac tggcacctta ccaacttgtt taaaaccaag
     8521 tttttcaaaa atatgttgca taaccttgtt ttcagcatgc gtatctgagc gaaaatcaag
     8581 ataatcaaaa ccttcaatca agccctctaa gaaggtttga gcaactcctt ttccctgcac
     8641 atctgctgcc acagcaatac ggtgaaagac tagatactct gactctccag cttgccagtt
     8701 tccctcataa atagcttcat aggcctcctc tggactcttg gtcacagcag cataggctag
     8761 cagttctccc tcttccaagg ctacgtaggc ttgccctgag atgatatcat cgataataac
     8821 atctgcattt ggatagccat tttgccactg gtcactacca gcatctgcta aacatttttt
     8881 ggcatcctcc atcacctgca tgatggcatc tacttcattt ggaaaagcta aacgaatttc
     8941 catctttttt cctcatttct gactagtttc tcccatcata gcataattta agtatttcta
     9001 caagcagtta aaacttgact ttgtgttttt gttattttat aatctagtta tcaaaactat
     9061 ataaaaagga gttgaaaatg aaaactacct tttcctaccc aaaatgggca gaaattccaa
     9121 acattgacct ctatctggac caggttttgc tctatgtcaa tcaggtctgc gcccctatct
     9181 ctcctaataa agacaagggc ctaacagcat ctatggtcaa caattatgtg aaaaatggtt
     9241 acctgacaaa gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt ttgattgcta
     9301 tcacaaccct caagtctgta ttttctattc aagaaatagc ccagacactt aatactctac
     9361 aaactcaagc aagttcagac caactctacg atgcttttgt ggactacatg aaccaaggaa
     9421 ttgacccagc taaccctatt atccaaacca gctgccaaac cgtcaaactc tatcatcaaa
     9481 ctctagactt aatcgatcat actcaagagg aggtaatcca atgaacacta gtcttaaact
     9541 cagcaaacaa ctcagttttg gagaggagat tgctaatagc gtgacccatg ctgtgggtgc
     9601 agtcatcatg cttatcttgc tgcctatttc atccatctat agttatgaag cacacggatt
     9661 tttatcctct atcggcgttt ccattttcgt catcagtctc tttctcatgt tcctatcatc
     9721 caccatttat cactctatgg cctatggttc gacccacaaa tatgttttgc gaatcattga
     9781 ccattctatg atttacgttg ccattgccgg ctcatacacg cccgttgtct tgaccttgat
     9841 gaataactgg tttggctatc tgattattgt catccaatgg ggaacgacca tctttggtat
     9901 tctctataaa atctttgcta aaaaggtcaa tgagaaattt agccttgctc tttacctgat
     9961 tatgggctgg ttggttctgg ctatcattcc tgccattatc agtcaaacga cacccgtttt
    10021 ctggagtctc atggtaactg gcggactctg ttatacagtt ggagctggat tttatgccaa
    10081 gaaaaaacct tatttccaca tgatttggca tctctttatc ctagctgcgt ccgcacttca
    10141 atacatcgct attgtttatt acatgtaaaa aagttgagaa attcaatctc aacttttttc
    10201 tttacacata ttgataaagt actggtgcaa gcgcacatca tcagtcaatt ctggatgaaa
    10261 agaacttacc aacatatttt tttcttgggc tgcaacaatt tgattgttca ctgttgctag
    10321 aatttctaca ccctcaccaa cactactgat aatcggacca cggataaagg tcattggaat
    10381 cttgccaact cccttacatt ctgcttccgt gtagaaactt cctaattggc gcccataagc
    10441 attacgctcg accaccatat ccatagttcc tagatgactc tctttctgag aagtgatttc
    10501 cttagccagc aaaattaagc ccgcacaggt cccaaacact ggtaagccag atagaatggc
    10561 ttctcggatg ggaagtagca tgttctggtc acgtaagagc ttgcccatgg ttgtagactc
    10621 accaccaggc aaaatcaaac ccgacaagtc actctgatct tgctgaaaat catctagatt
    10681 tctgagttct acactctcga cacctaattg atctagcact tttgcatgtt ctgcaaaggc
    10741 cccttgcaag gccaatattc cgattttcat ctattttcct cgttcagcca tgagaatttg
    10801 gatttcattt tcattaatac caaccatggc ttctcctaaa tcttcagaga tttgagctag
    10861 gatttgagga ttacggaagt tagtcacagc cttaacaatg gcactcgctc gtttaacagg
    10921 atctcctgac ttgaaaatac ctgaaccgac aaagaccccc tctgccccta attgcatcat
    10981 taacgcagca tctgctggcg ttgcaacacc tccagcagcg aaatttacaa ctggcaattt
    11041 tccatgttca tgaacatatt ggaccaattc tacagggact tgcaaatcct tggcagcaac
    11101 ataaagctcg tcctcacgta agttttgaat gcggcgaatt tcctgattca tcatacgcat
    11161 atgacgaaca gcttggacga tatcccctgt ccctggttct cctttggtac gaatcatgga
    11221 agcaccttca gcgatacgac gcaaggcttc acccaaatcc ttagcaccac agacaaaagg
    11281 aacttggaat tctttcttgt ccacatggaa acggtcatca gctggagata gaacttcact
    11341 ctcgtcgata taatcaattt caatagcctc taaaatctga gcttcaacaa aatgcccgat
    11401 tctgacctta gccattactg gaatactaac cgcttcttgg atttccttaa tcatctttgg
    11461 gtcgctcatg cgggaaactc ctccagctgc acgaatatca gccggaattc gttccaaggc
    11521 catcacagct gccgcaccag cagcttctgc gatacgagcc tgttcaggat tctgcacatc
    11581 cataataaca ccacccttga gcatctgtgc caagttttta tttagttcat aacgattttc
    11641 agtcatttgt tttatcctca tcatttgtat tggtagctac cttttcattt taagtcagaa
    11701 cagaatgaag cacaagataa aaaaactata attgtatggg gacagattgg ctaaaaatta
    11761 tctgacctta acttaaggcc agatagctca ttcatttcta tttttcagcc gtaagggcag
    11821 ccattgtgat gtagttgtat ggtttgttga agtgtggcaa gaagaagagg tctgtcaatg
    11881 ccaatttatc aattgtcaca tgctcttgga tagcaagtga gaacatgtgg attcccatgc
    11941 taattgcaat atcatgtgaa accatttggg caccaagaat ttcacggcta tctttgtcaa
    12001 agacaatctt aattgctact tcatggttgt catgtttcat gaattctggt ttttgaagat
    12061 cgttaaagcc tgtttcagtt gcgttgtaac cagcagcttt cgctttttca agagtcaaac
    12121 cagttgaaac catgtgaaga ccgtagattg agataccatt tgaaccttga acaccgattc
    12181 cttccaattc atgtccacaa gcattgtagg caccaacgat accagtgcga acagcatttg
    12241 aagcaagagc gatatagctt gtatctttac gagcattgtc ataaacagtc gcacagtcac
    12301 caacagcgta aacgcctggg atagatgttt cttgtttctt gtctacaagg aaggcaccgt
    12361 tgcggaagag ttcgatctta ccatctgcaa gggctgtgtt tggacggaaa ccaactgcaa
    12421 ggataaccat atccacgtca aagctttctt tgtcagtaat caagcgttca actttaccgt
    12481 caccttcgat tgctttaaca gtttgaccta gagccaagcg gatgttgtga tcttccaagt
    12541 tcttcgccat catttgtgtg aagtctttgt catagtaacc gttcaagaca gtatcaacga
    12601 tatcaacaag gacaacttct tttccaagac gttcaaaggc ttcagcaagt tcaacaccga
    12661 tgtaaccacc accaacaacg gcgatacggt cgaggtgttg gctcttgtca gaaagtttat
    12721 tgataacttc ttcagcattt tggtacaatt tcacgaattg tacgttttca agagttgctt
    12781 taaattcgcg gtttccttta acaatttcaa caccttcgat tggtggcaag attggtgtag
    12841 aacctgtagc gaaaatcaat ttttcgtatg attctttgtg ctcttttcct tcaacttccg
    12901 ctgtaactac tttgttatca tagtcgattg aaagaacagg tgagttcatg taaactttag
    12961 cacctttagc ttccaatttt tctttatcag aatagaacaa gccttcagca ccgtcaattt
    13021 gttcaccaat ccaaagagcc attccacatc ctaggaaaga gatgttagag ttttggtcaa
    13081 atacaacaat ttcgttctca tttccaaaat tatccaacat ggtattgata catgctgtac
    13141 cagcgtggtt agcaccgact acaacgattt tactcataaa aatttcctgc cttcattcta
    13201 aatttacctt actagtatac catttaccgt tagcgtttac aaggggggta ctattatttc
    13261 agcatttttt atcaaaaata attccccatt tatcctgttt tcaagcctga tgaattttat
    13321 gagcatattt cttgactttt tccaaatttt gtttgtgaaa attctaactt tatggtatta
    13381 taataacatg aaaatgaaat gtaagggctt caatttagat gattatttga tattacaact
    13441 tattttctat aagagaaagg agttggtagc ttgactcttc gttcacattc tgaacggcta
    13501 atggggacta ctatcactat ttcattagtg gatgagcaag ccgatatctt tctccaaaaa
    13561 tcctttgact tgctcaaaga acttgaatac cgcttcaacg ccaatagtca agaatctgag
    13621 ttgatggaaa tcaattatca agcaggaata gcccccgtca cggttcatcc agacctgttt
    13681 gagctgattt cacttgggtt agagcatagc ctagcgctct ctagccacct caatatcagc
    13741 attggtccct taattcaaac ctggcgtatc ggtttttcag atgccaaggt cgcgcagcct
    13801 caagaaattg aatcggtgct gcctctaatc aatcctcatg gtatcgagtt agattcttct
    13861 acttctactg tgtttttaaa acagaaagga atgaagatcg atctaggttg tttagccaaa
    13921 ggatacagtg cggataaggt tgcccaattt cttaggaaag agggggtgac ttctgccttg
    13981 atcaatctgg gagggaatat cctgaccatt ggaaaaaatc aggcaagagg ggataaccca
    14041 tggcaaatcg ggattcaaga cccagccaat cctaggggaa atcatttaat gaccatccct
    14101 gttgtcaata aatctgtcgt gacttcaggc atttatgaac gtcacctgac cgtcgatgga
    14161 caagattacc atcacatttt tgacagtcaa acaggatatc ctgttgaaac ggaactagcg
    14221 agtctaacaa tcatctctga taaatcagtc gatggcgaaa tctggacgac tcgactcttt
    14281 ggagaaagac ctgcttctat cctctggcaa gtcgaaagtt tggagggcat cgaagtcatc
    14341 ctcatcgata aagaaggcca cctaagctgt tcttcaggaa ttcctactct atagagacaa
    14401 ctgtttcaat ggaattttaa aattgtatta tgtaaaaaga gaaaggaaat ctcatgttaa
    14461 aacttattgc tattgttgga acaaattcaa aacgttctac aaaccgtcaa ttgcttcaat
    14521 acatgcaaaa acactttgct gacaaagctg aaattgaact tgttgaaatc aagaccattc
    14581 ctgtcttcaa caaaccagct gacaagcaag tacctgctga aatattggaa attgctgcta
    14641 aaatcgaaga ggcagatggc gttattatcg gtactcctga gtatgatcac tctattccag
    14701 ctgttttgat gagcgctctt gcttggttgt cttatggtat ttacccactt ttgaacaaac
    14761 caatcatgat tacaggtgct tcttacggta cgcttggttc atctcgtgcc caattgcaac
    14821 ttcgtcaaat cttgaatgct cctgaaatca aggcaaatgt tcttccagat gaattcttgc
    14881 tctcacactc tcttcaagca tttaacccaa gtggcgactt ggttgacctt gatgttatca
    14941 agaaattgga tgccatcttt gatgacttcc gtatcttcgt gaagattact gagaaattgc
    15001 gcaatgcgca agaattgctt cgcaaagatg ctgaagactt tgactgggaa aatttgtaag
    15061 ataggagacc aaaaagaatg aaatttgttg gacttgttgg atcaaactac gatcaatcat
    15121 ataaccgtaa gctcttggaa tttatccgtc gctacttcaa actcaaattt gaattagaag
    15181 ttcttgaaat tgacgaagtt ccaatgttta accaagatga aaaatgggat gaaagtttcc
    15241 aattacgtta tttatataac aaaattactc gtgcagatgg tgttatcatt gctactcctg
    15301 agcataacca cactatctca gcttcactca aatctgtact tgaatggctt tcatacgaag
    15361 ttcatccatt tgaaaacaag cctgttatga ttgtgggtgc atcatactat gaccaaggaa
    15421 catcacgtgc tcaagttcac cttcgtaaaa tccttgatgc tccaggtgtt aatgcctaca
    15481 cgcttccagg taacgaattc cttcttggta aagctaagga agcttttgat aacaacggaa
    15541 atatcaccaa cgaaggcact gttaaattcc ttgaaacttg cttagataac tttgttaaat
    15601 acgtaggagt cgtttcgaaa ttgaaaaaac caaaaccaat cgaatcagaa gacttggatt
    15661 gtggaaaacc aatcgctaca accatcacag aagttgaccc tgacgatcca gaatgggtag
    15721 aaaaagttgc agcattcact ggtgctgttt ctggtgatac ctatgtcaaa ttggaccacg
    15781 gtatcctaac agttaaccaa attgatatgt tcttgaaagc tatgccattt gaattgacat
    15841 acgctgacga caacaaccaa ttcctctact acaacaacgc tcaccaagat ccagacacta
    15901 tgttcgctaa acgtgtacca cctcaatcag gtagccgtat gtcgactgtt catggttctc
    15961 ttccaccagc acgtatgaag aacgtagagt gggttatcgg aacacttcgc aacggaaacc
    16021 aagaatacgt ccgtacgatc gttccaggtt ctcctgcagg tgttatcaac actcacaact
    16081 accaagcaat gtactatcct gatggatcat acgctggtat caatgaaatc gtctttaact
    16141 tccaaccatg gcttgactgg tacctaaaag agactggtca acgtttggtg ggtggtagcg
    16201 gaccatttgc tcctgctgcc ggaggtcatg gagacgccga tgctacttct ggcgcttctg
    16261 attcagggga tgctggaggc cacggtggtg acgcagacgc tacagccggt gcaagttact
    16321 aatgatagtt tttaggaacc attatggttc cttttttagc gaaaaaagac tagcaaacac
    16381 aaactgctag tctctttgtt atcgtttcat ctatctttgt taaaagaaag aatacatata
    16441 ctcaatgaaa atcaaagagc aaactaggaa gctagccgca ggctgtactt aagtacggca
    16501 aggtgaagct gacgtggttt gaatttgatt ttcgaagagt attattatga atttggatca
    16561 tcaagactac ggccatgtaa tcctttttca cgttgtactt ggcgtagttt ttctggtgta
    16621 acatcgtttc cttcttcgtc cacaattttg attccttcaa tgtggtgacg aacagcgcgg
    16681 cgataacctt cgatgtactc ctcacgtagt ttggcttgtt ccactttttc ttctggtgtt
    16741 aagccttctg tttttttctt tttagcaagc tcattgatac gagcaatttt tttcggatcc
    16801 atttcttctc ctctgtaata agataaagtc cgtttatcaa attttattta gtgttaattt
    16861 ggttaaaaca agcaaactta gctgctttct tggttagttg tgacaagatt gccaaacgat
    16921 tttgacggac agcctgatct tcagccatta ccatagtatt ttcaaagaaa gcatcaatga
    16981 ctgggctaag cgcaaagagt tgtttcaatt gctgacttgc aggtcctgat aaaacgagtg
    17041 tttctactgc ttctgccaaa gctttttctt ggtcattctc aaatagtgct gaatcaaccg
    17101 tagcaacccc ttctgccttc tcagccaggt taaaggcacg agaaagtgat tcaacagatg
    17161 gtttaaaatc ttcttccttg cttacttcta cgagagcact tgctgcttcc aacatatctg
    17221 ccacaacaaa gtttgaacct gcaagaactg cttctttgat atcttttgga gtagagccca
    17281 tcatcttatc aacacgagcc ttgataaagt ccataacctc tgctttattt tcataagtca
    17341 aactgtcaaa tttcaatgca taaaggctat caatcagctc atccatagca atgtgccaac
    17401 caaaggcatc caagatacga accacacctt gagttgcacg acgaagggca taagggtcat
    17461 tagaacctga tggaatcaat cctactgaga agaaactcaa aatcgtatcc aatttgtctg
    17521 caatggctag aactgcgccg accttgctct ctggaagttc tccttcagct gatgtaggca
    17581 tgtagtgttc acgaatagca gctgccaccg ctggagtttc accagcaaga agggtgtatt
    17641 tttcacccat aattccttgg agttcgtcaa attcaccaac catacctgtc aacaagtcaa
    17701 acttgtaaat ggctgctgca cgagcaaggt caactgtttc atccactgac aaactagctt
    17761 tttctgccaa aagtacagtg atttgacccg tacgaatcat gtgttcacga agggaaccaa
    17821 tcttctcatg gaaggtgaca ttgtttaatt tttcaacaag atctgaaatc accaattttt
    17881 ggtcttcacg ccagaagaat tctccgtctt ccaagcgggc taccaagact ttttcatttc
    17941 ctttgatgac atttttcaaa cgctctgcgt ttccgttacg aacagaaatg aagtttggca
    18001 agagttttcc atcttgatca cgaacaacaa agtaacgctg gtgttccttc atagaagtca
    18061 ccaagacttc ttctggaact tcaaggtatt tagcatcaaa acttcccatg aaggcagttg
    18121 ggtattcaac caaattcaag acttcattca gcaaatccgc atcaatttcg atacgtacac
    18181 catgttttgc ctcaatttcc ttgatttggt caacaatcat ttgctcacgt tcacatggat
    18241 ctgcgattac aaactgctta cgaaggtctt cttcatagct caatgctgac tgaattttgg
    18301 tttctttccc caagaaacga tggccacggc tcacacgact tcccttgata tcaaggaaat
    18361 ccaagtcaaa ctcttgctca tccaagagaa cagttaaagt gtgaacaggg cggatgtatt
    18421 caaagctatt tccagcccag tgcatgctga cagggaaagt caatgacttc aagacatcta
    18481 caacaccagg aacaatggct tcaactgatt ggccaacttc ttccttagtg acatagacat
    18541 attcttcacc cttaatttca cggaattcga tatcttcaac agtcaaacct ttcccacgga
    18601 caaatccttg agctgctttg gtgaagtttc catcactatc caaggcaatt ttctttgctg
    18661 gtcccttgaa atcttctgtc aaatcagact gtttgtctga taaaccagtc acacgaacag
    18721 ccaaacgacg tggtgttgag aaggtttgaa tggcttcaaa agacaggcgg ttttccttga
    18781 ggaaggctgc cattttttcg cctagttgtt tttcacttgg tgtgacaaca taggctggta
    18841 attcttcaag accgagttct actaataagt tttttgtcat gttttttctc cgaaaagtat
    18901 ctttttattt tccagtttgc cttatgtgat tggcacgcaa gcaaccaact tcgttgtttc
    18961 attgctaaaa ttggagccaa aagtctccaa ttttgcctag tatccttagt ttcactaagg
    19021 ataccttcat cactgcggca actggctcag ggctcactct gttcgcccat tttcgtaatt
    19081 tgtcactctc tttattctgc gtcttctgct aggagtttag ctcttgtttc ttcatccaaa
    19141 agtgggtagc ctaggcgttt gcgttctgcg acaaaggttt tggctacaac acgggccaag
    19201 ttacggatac gagcgatata gcctgcacgc tctgttacag atacggcacc acgcgcgtca
    19261 agcagattaa aggtatgtga acatttgaga acatagtcat aggcagggtg taccaagcct
    19321 tcttctaatg cacgaccagc ttctttttca aacttatcaa agttttcaag caacatttct
    19381 tggtccgaaa tttcaaatga atattttgag tgctcatact caggctggat aaagatttct
    19441 ccgtatttta caccatcagc ccactcgata tcatagacag agtctacttc ttgaatgtaa
    19501 gaagccaagc gctccaaacc ataggtaact tccgcagtca cagggccagt tgccaatcca
    19561 ccgacttgtt ggaaataagt gaactgagtg atttccattc cgtcaagcca aacttcccaa
    19621 ccaagaccag ctgaaccagt tgatgggttt tcccagttgt cctcaacaaa acgaatatcg
    19681 tgctccaaag gattgattcc caatttttcc aaagactcaa ggtaaagttc ttggatattt
    19741 gatggagaag gcttcatgac cacctggaat tggtggtgtt ggtagagacg gttagggttt
    19801 tccccataac gaccgtcagc aggacgacgt gatggctcta cataagctgc attccatggc
    19861 tcaggtccga tagcacgaag gaaagtgtaa ggactcattg ttcccgcacc tttttcatta
    19921 tcataagcct gcataagcat acaaccttgg tcattccaaa attgttgcaa agtaaggatg
    19981 atttcctgga aagtcaattt cttagacatt gtttactcct ttttatataa attacttgcg
    20041 acatgagtct gcctcgtcga tcatacgagg caagacgagt tagtactgcg tctagctcag
    20101 gcactctagt gctgagcgtg gggcagtccg actgtaagga ggtttctgcc actgacgcag
    20161 tgaaatgtca atttcttaga cattgtttac tcctttagtt tttatatttc tttttcaatt
    20221 aatttctgct gaacaaacca atctaacaag ggaaaagttt tttctgtatt tgcccataaa
    20281 tgataagaat ctaagaccgc ttgtacacta cctaaatttt ctaccaactt atctactgtc
    20341 aaaaagctgc gccagtattc ataaaaaata cttgcatagt tcttatcata agtcgaagaa
    20401 ccaaaatcat tcaatgaatg ccaaccatac ttcttctgaa aaagttctac gagagattga
    20461 ttacaaattt tttccgcttg aaattcctct tctgtcaaga aatacttgcg actaatatat
    20521 tcaaccatcc cctcttcgaa ccagatataa gagtcataac catcaaaatc atctaaaaaa
    20581 tgctccgacc aatgagctaa ctcatgtcct acaatctgta agaggaaatt ttcagataaa
    20641 gaatgatagt gactttctat tgcttgagtt tgctgagaac actcgtagtc catcaattga
    20701 tacaaataca actctttcca aacagctaaa tcaggcgcca taaccattcg tctattattt
    20761 gtataggctg gaacagcgct ttccctaata atctgtgtag cagcattaaa atctgaccaa
    20821 ataacggctt gcggtaaatc acagactgca agttcattct ttaaaaaaga ttgataatct
    20881 ttcaactttt cagtatttct tactacaaaa tcacgaaatg tagctagttg actatcgtct
    20941 tttacaagat aaaggttgtc cacggctctc ctttctttca cacaatagac tgaataagca
    21001 aaagaaccgc gtccgacaat cctaggttga tttaacctag gggcgtcaga aacgcggttc
    21061 caccctaatt tattacttca ttgtttttga aaatactaca gaaagcgcca gctcattatt
    21121 ttgccctact tggctcccac tatcccaagc tcgcttgaag aacgccataa gactttcttt
    21181 cctgctgact attatatcaa aaattagaat aatgtacaat tctttttatg attttctaga
    21241 aatccacatc atcaactcgt ggagcacccg attgaacagc aatcgttttt aagttttctc
    21301 tctcctcatg actcagttca attccaaagc aatcaagatt agcttgaata cgagaggttg
    21361 tgacagattt tggaagtggt aaaaatcctt ctgccaagct ccaggccaag gctatctgag
    21421 caatcgattt tccgtgattt gctgcgattt cttgcacttg cttgctatca aatagttctc
    21481 cctgaccaaa aggcccccaa gcttccaata aaattccctt ttcacgacag taagctacga
    21541 cttgatcttg atacacacct ggcgccaagc gaacttgatt gaccgcagga acgatagttg
    21601 cagtttcaag caaggcatcc aaatgatggg gaagaaaatt gctaacgccg atagcacgga
    21661 ttttcccttc ttgatagagg tcttccatcg ctctccaaac ttccgcattg cgagttttcc
    21721 atgcgtcatt ttctctgagc ggttttgggt tcggccaatg aatcaaatac aaatccaaat
    21781 aatccaagcc cagtttttct atagattttt ccaaagcttg acgagtttgc tcataggttt
    21841 gctgactatt ccaaagcttg gtagttacga acatttcttc acgtggaact ccgctatctt
    21901 tgattgcttg accaacactt tcttcattct gataaatcgc cgccgtatca atatgacgat
    21961 aaccagcctt caaggcttct aacactgcat gataggcttc ttctccatcc ttagccttaa
    22021 aagttccaaa tcccaatact ggaatttcta ctccattatt taattgatag gtattcatat
    22081 gcttctcctt ctattttatt catcaattat agcataagac attttttaag tagtactatc
    22141 ttctgtaaaa aataagtctg tcaaatatct aacagaccta ttttttcttg cttattcatc
    22201 acgactatag tacagctcat gagcttttag gcgagtattg agcatctctg gtatggtcac
    22261 aaaggtataa ccttgatttt tcaaatactc aatgaccctt ggcaaggcat tgactgtcgg
    22321 actgtgaata tcatgcatca aaacgataga gccgttagct acttgatgct gaatttctgt
    22381 caaaatagat gcttcatttt tactcttcca gtccagacta tccacatccc acatgataaa
    22441 gctcaaatcc aagctattgc gaatatcatc tgtaatagca ccataaggtg gacgcatgag
    22501 tttagaacta gaacccagca ctttagttag cacatcctca gtatcagtaa tctgcttttt
    22561 agcttcatca agagagagtt gcgagagaat cggatggctc caactatggt ttccaacaac
    22621 atgaccttca gattttatcc tcttcaccaa gtcctcattc ccagaaacat ttttcccaag
    22681 cacaaagaaa gtcgctttaa tatcatattt agctagggtc tctaatacct gcggggtcgt
    22741 tgctggattt ggaccatcat caaaggttag agcgacaact ttttgatatt tcttatcaaa
    22801 gtaagattgg tacaaggccg catctttttc gagtaagtac gaagattgga taacatcaaa
    22861 gaaagcagat actggcaagg ctatctcttc taaattttca agcacaggac ttggataaag
    22921 gataatctga ctatccttgt aatcaaaatt ccatgcagac aagtcttggt cagagaagtt
    22981 ttttacaatc tgctcactct ggtcttgctc tattttttta tcctctatga agggggtcaa
    23041 ctcttttatc agctgttcct tagccttact agcatctgaa aacagttggt caagtgtaaa
    23101 aggttgccca tcttctgtta agtgaacctt tcctagactg gtcttttcag tctcttcaac
    23161 cttaaaagca actaaatcat agatttgttt ggtcacatta cgattaacga ctccctttaa
    23221 acctgactct tcttgctctg tatagtagaa aacaagattg tccttacttt ctagcttgtc
    23281 cttaacatct tgatttatca actccctaac agaggaaatc actttctccc cttggagagg
    23341 ataataggca atcacttcgg cttgcccctg acgaaaatgc tccttctgat ttccctcact
    23401 caattgatca tctttctctt ttttgagcga ttcaatcttt tgctcaaaac ttttttgctg
    23461 gtagatctta aaagcaataa atccgcctaa taggcaaata gaaattgcta aaataccaat
    23521 caaagccaat aatacatgtc tcgttttccc gtgtctgcca cgtcctagtc tacttttatt
    23581 catatttata tcataacaaa tttgagcaat aatttcaatg atacaatttg gcaatccaca
    23641 attcctctag caagccaaag catcttacat caactccaac atccgtatct gtagagatta
    23701 tgtagttttc tatctatgga atttctagta gattgaagct agaatagtag acctatactt
    23761 ctaaaacatt gttagaaatc gatttgattg tcctgatcga tttgtcctgt tcttatttca
    23821 ttttactata aaactatgag aagctaggtc atctgagaag taattactat cgacaattgc
    23881 gatagcgtat actggtgcga tgtgtttgta gctctggtgg gtatcacctt cacgttggcg
    23941 aattttttct aggttttgat taacctgact gcacagataa gcccataggc gattgatgaa
    24001 aaaattctga tgatgaacct gaatttcgat gataacttgg attccattat ctaactccgc
    24061 caagacgtct atactagtat agaaatcctg cgctgagtag ggcatggaag gcaagacgtg
    24121 aatgttactt ccctccaaaa tcgtcacatt ttttgctggc aagtccagca tatcgcggat
    24181 aaattgacaa gtgatttctg gattgctgaa tattttttta gcaaccaaat cattagttgg
    24241 gctgatgccc ggatgtctga gaatcctcct tctattatag cacattttta aatctaggtt
    24301 tgctagattc tatgcttcta tctatttatt cggaaaagaa agctaatagc ttatttgaaa
    24361 ttttctcctc tctgttggat agtcccgtct atctatgtta taatgaaaga aggatttaaa
    24421 atcggaaaag gagtatttat gctaaaatta ggtgtcatcg gaactggcgc tatcagccat
    24481 catttcatag aagcagccca taccagtgga gaataccaac tggtcgcaat ctattctaga
    24541 aaactagaaa ctgcagcaac ctttgcttct cgctatcaga atatccaact attcgatcaa
    24601 ttagaagtct tcttcaaatc ttcttttgat ttagtctata tcgccagtcc aaactcctta
    24661 cattttgctc aggcaaaagc tgccttgtct gctggtaaac atgttattct cgaaaagcca
    24721 gctgtcactc aaccacaaga atggtttgat ttgattcaaa cagctgaaaa aaataactgt
    24781 tttatctttg aagcagctcg taattaccac gagaaagcct ttactactat caaaaacttt
    24841 ttagcagata agcaagtgtt aggagcagat ttcaactatg ccaaatactc ttccaagatg
    24901 cctgacttgt tgactggaca aacaccaaat gtcttttcaa accgttttgc tggtggagcc
    24961 ctcatggatt tggggattta tcccctctat gctgctgttc gtctttttgg aaaagctaat
    25021 gacgcgactt accatgctca acagcttgac aatagcattg acctaaatgg agatggtatc
    25081 ctcttctacc cagactatca agttcacatc aaggctggaa aaaacatcac ttccaatctt
    25141 ccttgcgaga tttatacaac agatggaacc ttgactctca acacgattga gcatattcgc
    25201 tcagctattt ttaccgacca ccaaggaaat caagtccagc ttcctatcca acaggctcct
    25261 catacgatga ctgaggaagt cgctgcattt gcacacatga tccagcaacc agatctgaat
    25321 ctctaccaaa cttggctgta tgatgcaggc tctgttcatg agctactata taccatgcgc
    25381 cagactgctg gcattagatt tgaggcagaa aaatgaaaac caaactaccg actgaatggc
    25441 aagaactgag tgaccaactc ggtttccaag aattcacccc cattcaaact caactatttg
    25501 agccccttct tgctggagaa aacctcctag gagtgagcca aaccggaact ggtaagaccc
    25561 tagcgtatct cctaccaagt cttctcagac tacaaaagaa aaaagcccaa caactcttga
    25621 ttctagcacc aaacacggaa cttgctggac agatttttga tgtatgtaaa acgtgggcag
    25681 aagctatcgg attgactgct caactcttcc tttcaggttc aagtcagaaa cgacaaattg
    25741 aacgccttaa aaaggggcca gaaattctga ttggaactcc tggccgtatc tttgaactca
    25801 ttaaacttaa aaaaatcaag atgatgaatg tggaaaccat catcctggat gaatttgacc
    25861 aattactaga tgattctcag attcactttg tagaaaaaat cactcactac gcacctcgtg
    25921 accaccaact ggtctacatg agtgccacga ccaagtttga tcaagaaaag attgtgccga
    25981 atacacgcac tattgatctc tctgatcaaa aattggacaa tatccagcat ttctacatgc
    26041 aggtagacca acgtcatcga gtggatatac tacgaaaact ggctcatgta gaggatttcc
    26101 gtggtctagt cttcttcaat agcctgtcag accttgggaa tgccgaggaa aaactacagt
    26161 atcgtgatat attggctgtt tccctcgcta gtgatgttaa tgttaaattt agaaaagtca
    26221 tcttagaaaa gtttaaagat aaccaactaa ccctgcttct tgcaactgac cttttggctc
    26281 gtggaattga tatcgatagc ctagaatgtg tcgtaaactt tgatattcct agagatagcg
    26341 aaacctacac acaccgtgct ggccgtactg gccgtatggg caaggaagga tatgttatta
    26401 ctctcgtcac tcatccagaa gaaatcaaaa aacttaaaaa gtttgcaagt atccgagaaa
    26461 ttgtcctaaa aaaccaagaa ctctatatca aataaagccc cattgctcta tacacatcca
    26521 gtgtatggag cttttttata agcaattcaa ttttatactc atctgctttc aaaaagcatt
    26581 ctattccatc tccgattaac gatggacttt atcacctcct tctccagtcc ttgtatgaca
    26641 tcttggagtt gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacgt
    26701 ttacgaatct ctttccacac ttgttcaatg ggttcatctc tggtgtgtat ggaggaataa
    26761 aggtaaaatc aatattagtc ggaatcttta aggtacttga tttatgccat atagcattgt
    26821 ccataacgag taaaaaataa tcatctggat aagcttgtga aagctcttct aaaaagacgt
    26881 tcatccactc agtattacat ccaccagcta ttaagaaaaa tgattcgcct gtatggggca
    26941 tcaacagctc cataacaata gcgaaattct cgtatatagt gactatggac atgtggacct
    27001 actcctattg gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct
    27061 tggtacatca agcgaacctt atgaaaaggt ctactggttt taaagcgctt tcttgtcttc
    27121 ttgaattgag attttatttt tagacgcgac aatggtttga gcgtctgctt tcttaggatg
    27181 ttctggacgt ggcataatat ttcgccaacc atggtgcttc aacagttgat agaaggcatc
    27241 acgtgtgtag gaacgatata agcctgaaat aaggtattgt aacaaattct cctgcctctg
    27301 tagccttcaa atggcgggca agaaaggctt tctcttcctc aactgtcata tatgcatggt
    27361 tacgaccacc acgtgtttct tgaaggagag agtcgagtcc gaactcctca tattttttta
    27421 catttcgcca aatcgttgtt tgattacagt ctaaaagatc tataatctct ttataagatt
    27481 tgcccatcag acgaaataga acgatttgaa ggcgtttatg atatttagct gtacgaaagt
    27541 cttttaaaag tgttttgatg gtttggattt cttcttcagt tgatttcata ttactattgt
    27601 ataatgcttt ttgatttgag tctagtatta tactcttcga aaatctcttc aaaccgcgtc
    27661 agcttcgcct tgtcgtatag atgttactga cttcgtcagt tctatctaca acctaaaaac
    27721 agtgttttga gcagtctgca gctaactttc tataacaaaa tgaaataaga acaagacaaa
    27781 tcaatcaaga ca
//
LOCUS       DAVLWA010000018        27281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLWA010000018 DAVLWA010000000
VERSION     DAVLWA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.018"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            584..805
                     /locus_tag="VYN80_000779"
     CDS             584..805
                     /locus_tag="VYN80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540724.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            1322..1575
                     /locus_tag="VYN80_000780"
                     /pseudo
     CDS             1322..1575
                     /locus_tag="VYN80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1703..2932
                     /locus_tag="VYN80_000781"
     CDS             1703..2932
                     /locus_tag="VYN80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1540725.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2952..5648
                     /gene="ppc"
                     /locus_tag="VYN80_000782"
     CDS             2952..5648
                     /gene="ppc"
                     /locus_tag="VYN80_000782"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1540726.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5731..5948
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6061..7065
                     /locus_tag="VYN80_000783"
     CDS             6061..7065
                     /locus_tag="VYN80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540727.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7134..8000
                     /locus_tag="VYN80_000784"
     CDS             7134..8000
                     /locus_tag="VYN80_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540728.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7997..8755
                     /locus_tag="VYN80_000785"
     CDS             7997..8755
                     /locus_tag="VYN80_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540729.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8845..10605
                     /gene="dnaG"
                     /locus_tag="VYN80_000786"
     CDS             8845..10605
                     /gene="dnaG"
                     /locus_tag="VYN80_000786"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1540730.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10608..11717
                     /gene="rpoD"
                     /locus_tag="VYN80_000787"
     CDS             10608..11717
                     /gene="rpoD"
                     /locus_tag="VYN80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1540731.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11731..12060
                     /locus_tag="VYN80_000788"
     CDS             11731..12060
                     /locus_tag="VYN80_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1540732.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12094..13218
                     /gene="cpoA"
                     /locus_tag="VYN80_000789"
     CDS             12094..13218
                     /gene="cpoA"
                     /locus_tag="VYN80_000789"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1540733.1"
                     /translation="MRKFPLFSSSLSFLLLILFKENDIIVVMEKKKLRINMLSSSEKV
                     AGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGR
                     KIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPR
                     EKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLA
                     EELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLF
                     LLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQAN
                     PAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK"
     gene            13234..14559
                     /locus_tag="VYN80_000790"
     CDS             13234..14559
                     /locus_tag="VYN80_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1540734.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14590..14766
                     /locus_tag="VYN80_000791"
     CDS             14590..14766
                     /locus_tag="VYN80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1540735.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVTWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14825..14959
                     /locus_tag="VYN80_000792"
     CDS             14825..14959
                     /locus_tag="VYN80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1540736.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15023..16333
                     /gene="obgE"
                     /locus_tag="VYN80_000793"
     CDS             15023..16333
                     /gene="obgE"
                     /locus_tag="VYN80_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1540737.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGGDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16343..16501
                     /locus_tag="VYN80_000794"
     CDS             16343..16501
                     /locus_tag="VYN80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540738.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17588..18847
                     /locus_tag="VYN80_000795"
     CDS             17588..18847
                     /locus_tag="VYN80_000795"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1540739.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEIGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18863..19414
                     /locus_tag="VYN80_000796"
     CDS             18863..19414
                     /locus_tag="VYN80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540740.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19407..20684
                     /locus_tag="VYN80_000797"
     CDS             19407..20684
                     /locus_tag="VYN80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1540741.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20700..21560
                     /locus_tag="VYN80_000798"
     CDS             20700..21560
                     /locus_tag="VYN80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1540742.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21941..22726
                     /locus_tag="VYN80_000799"
                     /pseudo
     CDS             21941..22726
                     /locus_tag="VYN80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            22917..25208
                     /gene="pcrA"
                     /locus_tag="VYN80_000800"
     CDS             22917..25208
                     /gene="pcrA"
                     /locus_tag="VYN80_000800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1540743.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNNLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            25251..25931
                     /gene="radC"
                     /locus_tag="VYN80_000801"
     CDS             25251..25931
                     /gene="radC"
                     /locus_tag="VYN80_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1540744.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(25928..26617)
                     /locus_tag="VYN80_000802"
     CDS             complement(25928..26617)
                     /locus_tag="VYN80_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1540745.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELTLIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(26635..27276)
                     /locus_tag="VYN80_000803"
     CDS             complement(26635..27276)
                     /locus_tag="VYN80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1540746.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
BASE COUNT         8321 a         4811 c         6050 g         8099 t
ORIGIN      
        1 cttccaaaac taataaaatt gaaagaacaa aatctagttt aaatgttgct cgaaaattaa
       61 ataattgaat acgattaaca atgaaacaat acaacgtgag tcatgacttc aaagtaattg
      121 attttatgat tctggtttct tacgcttaat taagcaagtt gtattcactt tatcaaattg
      181 ttattactct aaaaagcgtc taacagctat agagtgtctg tttgcttctc tttactttta
      241 ttttaaataa ttcattaacc ttccgtaagt gcccaataat aaggtgtgtt gaagaagtct
      301 gagataaaat agttctcagt cacaaaaaag ctatagattc ccattggtga acagctccct
      361 gtcaagtaga cagtaaaata atataaaatc ttaagcgacc tgattcctga attccagtgg
      421 agtcaggttg ttttttaacc gtctttctta atattgtctt acctataata gatatgctta
      481 tgagatatgt ttaataaatt aattttagtt caaaatgtcc agttaggaca ctttgaatga
      541 tttcttagtt ttatatatta tacttttctc aggagaaagc tagatgtaca gacgtttgag
      601 agatttgagg gaagatcacg atttgaccca aaaacaaata gctaaaatac tttcgtttac
      661 aaactcagct tatgctaaaa ttgaacgggg tgagcatacg ttaacagcag atgtattggt
      721 aactctctca aacttttacg atgtcagtac agactaccta ttggaattga cagattttcc
      781 tgataaaatt cgctttagaa aataatctcc tcaatttcat agagtgtgaa aatgaggaga
      841 ttttttattt gttctttgaa aattaaatag tccaaaatgg tactttcctc atttgtggag
      901 caaatttgaa tggctcgcca tgataagagc gattttaaaa ccatcaataa aatagagcga
      961 tactttatat gccatgatac aaatgatata caatgatact tctgaccgtt caggctgcca
     1021 tcgtaaaaga gcagcaagtg aaattcttat gatgacttca tcagtcatgc cacgttaaat
     1081 gtgtgagttt gttagagaaa cgcaattaat cctcaaaagg ttccccgaac cttttgagtt
     1141 ccacagacgc atcacgtgga gtgtgtaagc ttgttgctaa aagcgtaaaa accttggaac
     1201 gaaaggaata atagacttcc tgcgaaacaa aaatacaata caataaaact atggatgatg
     1261 aagcaagtaa acaattaagc gatagccgtt tcaagagcct tgtaggtgtt cagcgcacga
     1321 gatgttagct gtgttaaaaa cagcttatca acgcaacgta aacgcgcaaa aggtggacga
     1381 aaaaccaaat taagcctaga cgatctcctc atggtaacta ttcaatacat gcgagaatag
     1441 cgcacttatg aacaaattgc ggctgatttt ggcattcacg aaagcaactt aatccgtcgg
     1501 agccaatggg ttgaagtaac tcttgttcaa aatggtttta cgatttcaaa ttctgcccta
     1561 attatgaaaa acagtaaaat tcgaaggatt gtaaggtaag agtttttttc tttctgaaaa
     1621 aatggtataa tagcaatcaa aactagaaaa taaaacggaa tttggaacag atttgtctgt
     1681 atcctagtag agtggtgatg ctatgaagat tagtaagagg cacttattaa attattccat
     1741 cttgattccc tacttgcttt tatctatttt gggcttgatt gtggtctatt cgaccaccag
     1801 tgctatttta attgaagaag gcaagagcgc cttgcagttg gttcgaaacc aaggaatctt
     1861 ttggattgtt agtttgatac tgattgcctt aatttataaa ttgagactag attttttgag
     1921 aaatgagcga ctaatcattt tagttatatt aatagaaatg cttttattgt tcttggctcg
     1981 ttttattggt atttccgtaa acggggcata cggttggatt tcggttgcag gagtaactat
     2041 tcagccagct gagtacttaa aaatcattat tatttggtat ttagctcacc gattctccaa
     2101 acagcaagaa gaaatagcta cttatgattt tcaagttttg actcaaaatc aatggcttcc
     2161 ccgtgctttt aatgattggc gattcgttct cctagttctg attggaagtt tgggaatttt
     2221 ccctgattta ggaaatgcga ctattttagt cttggtttcc ttgattatgt atacagttag
     2281 tggaatcgct tatcgctggt tttcaaccat tctggcgctc gtatctgccg cttctgtctt
     2341 tgtcttgacc actatcagcc taatcggtgt tgagaccttt tcaaaaattc cagtatttgg
     2401 ctatgtagcc aagcgcttta gtgccttttt taatcctttt gccgatcgtg ctgatgcagg
     2461 tcaccagtta gctaattctt attttgccat ggtcaatggt ggttggtttg gtctaggtct
     2521 tggaaactcg attgaaaaac gaggttattt gccagaagct catacagact ttgtcttttc
     2581 tatcgtgatt gaagaatttg gctttgctgg tgccagtctt attttagctc tcttgttttt
     2641 catgattttg cggattatct tggtcggtat tcgagcggag aatcctttca atgccatggt
     2701 tgcactcggt gtcggaggga tgatgttggt tcaggtattt gtcaatatcg gagggatttc
     2761 gggcttgatt ccatctacag gagtaacctt ccccttctta tcccagggtg gaaatagtct
     2821 tctagtctta tcagtggcag tagcctttgt cttaaatatt gatgccagtg aaaaacgcgc
     2881 taagttgtac cgagaattgg aaaatcaacc aatgaacctt ctgttgaagt aggataaaga
     2941 aaggatagtt tatgtctctt caaaaattag aaaattatag taataaaagt gttgtgcaag
     3001 aagaagtctt gattctaaca gaattactgg aagatattac taaaaatatg cttgccccag
     3061 agacctttga aaaaataata cagttgaaag aattatcaac gcaggaagat tatcaaggtc
     3121 taaaccgtct agtgactagc ttatcaaatg atgaaatggt ctatatttca cgctatttct
     3181 ctatcttacc tcttttgatc aatatttcag aggatgtgga tttagcttat gaaatcaatc
     3241 atcaaaataa tattgatcag gactatttag gtaaattatc tacaacgatt aaattggtag
     3301 cagaaaagga aaatgccgtt gagatcctag aacacttgaa tgttgtccct gttttgacag
     3361 cccatccaac acaagtgcaa cgcaaaagta tgttggattt aacaaatcat attcatagtc
     3421 ttttgcgtaa ataccgtgat gttaagttgg ggttgatcaa taaagataaa tggtacaatg
     3481 atttgcgtcg ttacatcgaa attatcatgc agacagacat gattcgtgag aaaaaattaa
     3541 aagtgactaa cgaaatcacg aatgctatgg aatattataa cagctccttt ttgaaagctg
     3601 tacctcattt gacgacggag tataagcgct tagcgcaagc gcatggtctg aatttaaaac
     3661 aggctaaacc aatcaccatg ggtatgtgga taggtggtga ccgtgatgga aatccatttg
     3721 ttacagcaga gaccttgaag cagtctgcac tcactcaatg tgaagtcatc atgaactact
     3781 atgataaaaa gatttaccaa ctttatcgtg aattttctct ttcaactagc attgtcaacg
     3841 tcagcaagca agtcagagaa atggctcgtc aatccaagga taactcgatt taccgcgaaa
     3901 aagagcttta ccgtcgtgcc ttgtttgata ttcaatcaaa aattcaggca actaaaacct
     3961 atctgattga ggatgaagaa gttgggactc gttatgaaac cgccaatgat ttctacaagg
     4021 atttgattgc cattcgagat tctctactag aaaataaggg tgagtccttg atttcaggtg
     4081 attttgtgga attattgcag gcagtagaga tatttggttt ttacctagca tcaattgata
     4141 tgcgacaaga ctctagcgtc tatgaagcct gtgtggcaga actcttgaaa tcagcaggaa
     4201 ttcattctcg ttatagcgag ttgagcgaag aagaaaagtg tgaccttctc ttgaaagaat
     4261 tagaagaaga tccccgaatt ctttctgcga ctcacgcaga aaaatcagaa ttattagcaa
     4321 aagaattagc tatttttaag acggctcgtg ttttgaaaga taagttggga gatgatgtca
     4381 tccgtcagac catcatttca catgcaacca gcctttctga tatgctagaa ttagctattc
     4441 tgttaaaaga agtaggactg gtggatacgg aaagggcgcg tgttcagatt gttccccttt
     4501 ttgaaacaat tgaagacttg gatcattcag aagaaacaat gagaaaatat ctttctctta
     4561 gccttgccaa aaaatggatt gactcacgaa ataactacca agaaatcatg cttggctact
     4621 ctgacagtaa taaagatggc ggttacttgt catcatgttg gaccctctac aaggctcaac
     4681 aacaattgac tgctattgga gatgaatttg gcgttaaggt taccttcttc catggtcgtg
     4741 gtggtactgt cggtcgtggt ggtgggccaa cctatgaagc cattacatct caaccgctca
     4801 agtctatcaa ggatcgtatc cgcttgacgg agcagggtga agtaattggg aataaatacg
     4861 gtaacaaaga cgccgcttac tataaccttg aaatgctagt atcggcagct attaaccgta
     4921 tgattactca gaagaagagc gataccaata ccccaaatcg ttatgaagcc attatggatc
     4981 aagtagtgga ccgtagttac gatatctacc gtgatttggt ctttggtaat gagcatttct
     5041 atgattattt cttcgagtca agtccaatca aggctatttc aagttttaat attggttctc
     5101 gtccagccgc tcgtaagact attactgaaa tcggtggttt gcgtgccatc ccttgggtat
     5161 tctcatggtc acagagtcgt gttatgttcc ctggatggta cggggttggt tcaagcttca
     5221 aggaatttat caataaaaat ccagagaata ttgctatctt acgagatatg taccaaaatt
     5281 ggcctttctt ccaatcgctt ctttcaaatg ttgatatggt tttgtcaaaa tcaaatatga
     5341 atattgcttt tgaatatgct aaactttgtg aagacgagca agttaaggcc atctatgaga
     5401 ctattttaaa tgaatggcaa gttactaaga acgttatctt ggctattgaa ggacatgacg
     5461 aactcttagc tgacaatcca tatctaaaag ctagtctgga ttaccgtatg ccttacttta
     5521 atattctcaa ctatattcag ttagagttga ttaaacgcca acgtcgtgga gaattgtcca
     5581 gtgatcaaga acgattgatt catatcacca tcaacggaat tgcgacagga ttgcgtaatt
     5641 caggttgata attttcaaga gtgaatgcta aaagtgaata tcaaaaaaat tctaatagac
     5701 tattgacaag tagtttaaaa atgatataat ttaaccattc agaaaagtaa tcatacaaac
     5761 tttttagaga gtctgtggta gctgaaaaca gataagtggc gatgatgaaa attgggctga
     5821 atgctattta gaatttgaaa ttataaaaat tcggtaagca caccttacag tgcatctcgt
     5881 tattgcgaga ctgagcgata gggaaattcc ctataattga ggtggtaccg cgcatcgacg
     5941 tcctcacaca agttttttgt gtgaggattt ttttgatgga ggttagtatg gaaagaaaac
     6001 gatggcgtcg cttgtttaga taagtgaaat atgttaaagg aaataaaaag gagaaacaga
     6061 atgaaaaata aacgtttaat tggaattatt gctgcattag cagtcttagt agcaggaagc
     6121 ttgatttatt cttcaatgaa taaatcagaa gctcagaata ataaggatga gaagaaaata
     6181 actaagattg gtgtgcttca atttgtgagc catccatccc ttgatttgat ttataaaggg
     6241 atccaagatg gacttgcaga agaaggatat aaagatgatc aagttaaaat tgattttatg
     6301 aactcagaag gtgaccaaag taaggttgcg acaatgagta aacaattggt tgcaaatggg
     6361 aatgaccttg tggttggtat cgcaacacca gcagcccaag ggttggctag tgcaacaaaa
     6421 gacctaccgg ttatcatggc cgctattaca gacccaattg gtgctaactt ggttaaagat
     6481 ttgaaaaaac caggtggcaa cattacaggg gtatctgacc acaatccagc tcaacaacaa
     6541 gttgaactca tcaaggctct gacaccgaat gtgaaaacaa tcggagctct ttactcaagt
     6601 agcgaagaca attcaaaaac acaggtcgaa gaatttaagg cttatgctga aaaagcaggt
     6661 ctgacagtgg aaacatttgc agttccttca acaaatgaaa ttgcctcaac tgtcactgtt
     6721 atgactagca aggtagatgc tatttgggtt ccaattgata acaccattgc atcaggattt
     6781 ccaacggttg tctctagcaa tcaaagttct aagaaaccaa tttatcccag tgcgacagct
     6841 atggtagaag taggtggttt ggcatcagtt gtaattgacc aacatgacct tggtgtggca
     6901 acaggtaaaa tgattgtgca agtcttgaaa ggtgcaaaac cagccgatac cccagtcaat
     6961 gtcttttcaa ctggtaagtc agtcatcaat aaaaaaatag cacaagaact aggtattact
     7021 attcctgagt ctgttctcaa agaagcagga caagtcatcg aataaataag gtagggagga
     7081 ggagactctt cccatttttt aaaaagatga atatagaaag gattaaataa aatatgattg
     7141 tttccattat ttctcaagga tttgtctggg ctattctagg tctgggaatc tttatgacat
     7201 ttaggatttt aaactttcca gatatgacga cagaaggttc cttccctctt gggggagctg
     7261 ttgctgtcac tttgataacc aaaggcgtga acccattttt agcgacactt gttgctgtag
     7321 gagcaggttg tttggctgga atggcagcag gccttcttta tacaaaaggg aagatcccaa
     7381 ccttgctctc agggattttg gtgatgactt cttgtcactc aatcatgctc ttgattatgg
     7441 gacgtgcgaa tttaggcctg cttggaacca agcaaattca ggatgttttg ccttttgatt
     7501 cggatttgaa tcaactcttg acaggtctca tctttgtgag tattgttatt gctctcatgc
     7561 tctttttctt ggacactaaa ctcggacaag cctatattgc tacaggggat aatcctgata
     7621 tggctagaag tttcgggatt catactggac gcatggagct catgggcttg gtcttatcaa
     7681 atggtgtgat tgcccttgca ggtgccctca ttgctcagca agaaggttat gccgatgtgt
     7741 ctcgagggat cggggttatc gttgtggggc ttgcaagttt gattattgga gaagttattt
     7801 tcaagagttt gagcttggca gagcgtttgg ttactatcgt tgtaggttct atcgcttatc
     7861 aatttttagt gtgggcagtt atcgcacttg gctttaatac aagttacctt cgtttataca
     7921 gtgccttgat tttagcagtc tgcctcatga ttccaacatt taagcaaaca atcttgaaag
     7981 gagccaagtt aagcaaatga cagcaattgt agaattaaaa aatgcaacca aaatcgttaa
     8041 aaatggcttt gatgaagaaa agattatttt aaatgatgtt tccttagaaa tttttgaacg
     8101 ggactttatc acgattttgg gcggaaatgg tgctggaaaa tcaaccctct ttaacactat
     8161 agcagggacc ttatcactaa ctagtggaac tatccgtatt ttaggtgaag aactcactaa
     8221 gttttcaccc gagaagcgtg ccaagtacct gtctcgtgtc ttccaagatc caaagatggg
     8281 gacagctccc cgtatgacgg tcgctgaaaa tcttttaatc gccaagtttc gtggtgaaaa
     8341 gcgtggattg ttaccacgac gcttgactag ctataaggat gaatttcagg caaccattga
     8401 aaaagtagga aatggtcttg agaaacactt gaatacaccg attgagttct tatcaggtgg
     8461 acaaagacag gctttgagtc tcttgatggc aaccttgaag cgacctgaat tactcctgtt
     8521 agatgagcat actgctgccc tggatccaaa gactagtgtt gctttgatgg aattgacaga
     8581 tgaatttgtt aagaaagatc agctaacagc ccttatgatt actcatcata tggaagatgc
     8641 tctcaaatac ggcaatcgct tgattgtcat gaaagaagga cggattatcc aagatttaaa
     8701 ccaagaagaa aaagcaaaaa tgaaaatctc tgattattat caactctttg aataaagtag
     8761 ggaaaatgag tgaaatggtt tacttttttt ctgaaataaa gtatactata taaagtaaac
     8821 tatgataaca tggaggtatt gtgtatggtt gacaaacaag tcattgaaga aatcaaaaac
     8881 aatgccaaca ttgtggaagt cataggagat gtgatttctt tacaaaaggc aggacggaac
     8941 tatctagggc tctgtccttt tcatggtgaa aaaacacctt ctttcaacgt tgtagaggat
     9001 aagcagtttt atcactgttt cggttgtggt cgctcaggtg atgtctttaa gttcatcgag
     9061 gagtaccaag gggttccctt tatagaggca gtccaaatct taggtcagcg tgttggaatt
     9121 gaggttgaaa aaccgcttta tagtgagcag aagccagcct cacctcacca agctctttat
     9181 gatatgcacg aagatgcagc caaattttac catgcgattc tcatgacaac gactatgggc
     9241 gaagaggcca gaaattacct ttatcagcgg gggttgacag atgaagtgct taaacatttt
     9301 tggattggtt tagcacctcc agaacgaaac tatctctatc aacgtttgtc tgatcagtat
     9361 cgtgaagagg atttactgga ttcaggcctg ttttatctct cggatgccaa tcaatttgta
     9421 gacacctttc acaatcgcat tatgtttccc ctgacaaatg accaaggaaa ggtcattgcc
     9481 ttctcaggtc gtatctggca aaaaacggat tcacaaactt ctaagtataa aaacagccga
     9541 tcgactgcaa tttttaacaa aagttacgaa ttatatcata tggatagggc aaaaaaatct
     9601 tctggaaaag ctagtgagat ttacttgatg gaaggattca tggatgttat tgcagcctat
     9661 cgggctggaa tcgaaagtgc tgtggcgtcg atgggaacgg ccttgagtcg agagcatgtt
     9721 gagcatctga aaaggttaac caagaaattg gttcttgttt acgatggaga taaggctggg
     9781 caagccgcga cattgaaagc attggatgaa attggtgata tgcctgtgca aatcgtcagc
     9841 atgcctgata acttggatcc tgatgagtat ctacaaaaaa atggtccaga agacttggcc
     9901 tatctattaa cgaaaactcg tattagtccg attgagttct acattcatca gtacaaacct
     9961 gaaaacagtg aaaatctgca ggctcagatt gagtttcttg aaaaaatagc tcccttgatt
    10021 gttcaagaaa agtccatcgc tgctcaaaac agctatattc atattttagc tgacagtctg
    10081 gcgtcctttg attataccca gattgagcag attgttaatg agagtcgtca ggtgcaaagg
    10141 cagaatcgca tggaaggaat ttccagaccg acgccaatca ccatgccaat caccaagcag
    10201 ttatcggcta ttatgagggc agaagcccat ctactctatc ggatgatgga atcccctctt
    10261 gttttgaacg attaccgttt gcgagaagac tttgcatttg ctacacctga atttcaggtc
    10321 ttatatgact tgcttggcca gtatggaaat cttcctccag aagttttagc agagcagaca
    10381 gaggaagttg aaagagcttg gtaccaagtt ttagctcagg atttgcctgc tgaaatatcg
    10441 ccgcaggaac ttagtgaagt agagatgact cgaaacaagg ctctcttgaa tcaggacaat
    10501 atgagaatca aaaagaaggt gcaggaagct agccatgtag gagatacaga tacagcccta
    10561 gaagaattgg aacgtttaat ttcccaaaag agaagaatgg agtaataatg gcaacaaaac
    10621 aaaaagaagt aacaacattt gacgtacagg tagcagaatt tatccgtaat cataagcaaa
    10681 aagggacagc aacagatgat gaaatcaatg caagtctggt tattcctttt accttggacg
    10741 ctgatgggat tgaagatctc ttgcaacgga ttcaggatgc aggaatttct atcacagata
    10801 acgaaggaaa tccaagtgcg cgtgttctta gcaatgaaga agaaccagaa ctcagcgatg
    10861 aggacttgat tgggtcaact tctgctaagg tcaatgaccc tgtccgtatg tacttgaaag
    10921 aaataggggt cgttcctctc ttgaccaatg aagaggagaa agagttggca ctggctgttg
    10981 aagctggtga tatcgaagcc aaacaacgtc ttgcggaagc caatcttcgt ttggttgttt
    11041 ccattgccaa acgctatgtc ggtcgtggta tgcagttcct tgacttgatt caagaaggaa
    11101 atatgggctt gatgaaggcg gttgacaagt ttgactattc taaagggttc aagttttcaa
    11161 cttatgcaac ttggtggatt cgtcaggcta tcactcgtgc tattgcggac caagctcgta
    11221 ccatccgtat cccagttcac atggttgaaa ctatcaataa attggttcgt gaacagcgga
    11281 atctccttca agaattgggg caagatccga caccagaaca gattgctgaa cgaatggata
    11341 tgacacctga taaggttcgt gaaatcttga agattgccca agaaccagta tctcttgaaa
    11401 ctcctatcgg tgaagaggac gatagccacc ttggagactt tatcgaagat gaagtgattg
    11461 aaaatccagt ggattatacg actcgtatcg tcttgcgtga gcaattggat gaaatcttag
    11521 atactcttac agaccgtgaa gaaaatgttc tgcgtctacg ttttggacta gatgatggaa
    11581 aaatgcgcac acttgaagat gtggggaaag tctttaacgt aactcgtgag cgtatccgtc
    11641 agattgaagc aaaggctttg agaaaactac gccaaccaag tcgtagcaaa ccgcttcgtg
    11701 attttattga agactaagag tgaggaaaat atggcttata cagaagagca aattgaaaac
    11761 atcaaaacac ggattttaac agccttggaa gaagtcatcg accctgagtt gggaatcgat
    11821 attgtcaatc ttggtttgat ctatgagatt cgttttgacg gtgacacagg gcaaacagag
    11881 attgacatga ctttgacaac tatgggttgt cccttagcag accttttgac agaccagatt
    11941 tatgatgcta tgatagaggt tccagaagta acggatactg aggttaaatt agtctggtat
    12001 ccagcctgga cagttgaaaa aatgagtcgc tatgcccgca ttgccctagg cattaagtaa
    12061 acatataaaa gcatgtgaga gactgttgga aaagtgagga aatttcccct cttttcctct
    12121 agtctctcct ttcttttgct gattttattc aaagaaaatg atataatagt agttatggag
    12181 aaaaagaaat tacgcatcaa tatgttgagt tcaagtgaga aagtagcagg acagggagtt
    12241 tcaggtgctt accgtgaatt agttcgtctt cttcaccgtg ctgccaagga ccaattgatt
    12301 gttacagaaa atcttccaat cgaggcagat gtgactcact ttcatacgat tgattttccc
    12361 tattatttat caaccttcca aaagaaacgc tcagggagaa agattggcta tgtgcatttc
    12421 ttgccagcta cacttgaggg aagtttgaaa attccatttt tcttaaaggg aattgtgaaa
    12481 cgctatgtat tttcttttta caaccggatg gagcacttgg ttgtggtcaa tcctatgttt
    12541 attgaggatt tggtagcagc tggtattcca cgtgaaaaag tgacctatat tcctaacttt
    12601 gtcaacaagg aaaaatggca tcctctacca caagaagagg tagttagact gcgcacagat
    12661 cttggtctta gtgacaatca gtttatcgta gtaggtgctg ggcaagttca gaaacgtaaa
    12721 gggattgatg actttatccg tctggctgag gaattgcctc agattacctt tatctgggct
    12781 ggtggcttct cttttggtgg tatgacagat ggttatgaac actataagaa aattatggaa
    12841 aatcccccta aaaatttgat ttttccaggc attgtatcgc cagagcggat gcgcgaattg
    12901 tatgctctag cggatctttt cttgttgcct agttacaatg agctctttcc tatgactatt
    12961 ttagaagctg cgagttgtga ggctcctatt atgttgcgtg atttagatct ctataaggtg
    13021 attttggagg gaaattatcg ggcgacagcg ggtagagaag agatgaaaga ggctattttg
    13081 gaatatcaag caaatcctgc tgtcttaaaa gatctcaaag aaaaggctaa gaatatttcc
    13141 agagagtatt ctgaagagca tctgttacaa atctggttgg acttttatga gaaacaagcc
    13201 gctttaggga gaaagtaaaa agtgaggtaa tctatgcgaa ttggtttatt tacagatacc
    13261 tattttcctc aggtttctgg tgttgcgacc agtattcgaa ccttgaaaac agaacttgaa
    13321 aagcagggac atgctgtttt tatctttacg acgacagata aggatgtcaa tcgctacgaa
    13381 gattggcaaa ttatccgcat tccaagtgtt cctttctttg cttttaagga tcgtcgcttt
    13441 gcctaccgag gttttagcaa ggcacttgaa attgctaaac agtatcagct agatattatc
    13501 catactcaga cagaattttc tcttggcctg ttggggattt ggattgcgcg tgaattgaaa
    13561 attccagtca tccataccta tcacacccag tatgaagact atgtccatta tattgctaag
    13621 gggatgttga tccggccgag tatggtcaag tatctggtta gaggtttcct gcatgatgtg
    13681 gatggggtta tttgccctag tgagattgtc cgtgacttgc tatctgatta taaggtcaag
    13741 gttgaaaaac gggtcattcc tactgggatt gaattagcca agtttgagcg tccggaaatc
    13801 aagcaggaaa atttgaaaga actgcgtagt aaactaggga ttcaagatgg tgaaaagacg
    13861 ttgcttagtc tttcgagaat ctcctatgaa aaaaatattc aagcagtttt agcagccttt
    13921 gctgatgttc tgaaagagga agacaaggtt aaactggtag tagctgggga tggcccttat
    13981 ctgaatgacc tcaaagagca agctcagaac ctagagattc aagactcagt catctttaca
    14041 gggatgattg ctcctagtga gacggctctt tactataaag cggcggattt cttcatttcg
    14101 gcatcgacaa gcgaaacgca aggtttgacc tacttggaaa gcttagccag tggaacacct
    14161 gtcattgctc acggaaatcc ttatttgaac aacctcatca gtgataaaat gtttggaacc
    14221 ttgtactatg gagaacatga tttggctggt gctattttgg aagccctgat tgcaacacca
    14281 gacatgaacg agcatacctt atcagagaaa ttgtatgaga tttcagctga gaactttggg
    14341 aaacgagtgc atgagtttta tctggatgcc attatttcaa ataacttcca gaaagatttg
    14401 gctaaagatg atacggtcag tcagcgtatc tttaagacag ttttgtatct tcagcaacag
    14461 gtggttgctg tacctgtaaa aggatctaga cgcatgttga aggcttcaaa aacacagttg
    14521 atcagtatga gagactattg gaaagaccat gaagaataga aagaggaaca gctatgaaaa
    14581 aaacaattaa tgagaagcgg tcgtgataaa aagattgcgg gtgtttgtgc tggggtggcc
    14641 cattatctgg atatggatcc gactatcgtt caagtcactt ggggtgttct tacttgctgt
    14701 tacggagctg gaattgtagc ttacattatt ttatggatta tcgcaccagt agcaactgac
    14761 tattgaaaac tagctcaatt catgctaaga taatagaaaa tagaatgtaa cgaaggagag
    14821 aaaaatggca tttggagata atggaaatcg taaaaaaact atgtttgaga aaataacctt
    14881 gtttatcgtg attatcatgc tagtagcaag tttattggga atttttgcaa ctgcaattgg
    14941 tgccctcagt aatctataaa atagattcaa gaaaatttag tgactgggat ttcccagccc
    15001 ttttttaaag tgagaagaaa taatgagtat gtttttagat acagctaaga ttaaggtcaa
    15061 ggctggtaat ggtggcgatg gtatggttgc ctttcgtcgt gaaaaatatg tccctaatgg
    15121 aggcccttgg ggtggtgatg gtggtcgtgg aggcaatgtg gtcttcgttg tagacgaagg
    15181 actacgtacc ttgatggatt tccgctacaa tcgtcatttc aaggctgatt ctggtgaaaa
    15241 agggatgacc aaagggatgc atggtcgtgg tgctgaggac cttagagttc gagtaccaca
    15301 aggtacgact gttcgtgatg cggagactgg caaggtttta acagatttga ttgaacatgg
    15361 gcaagaattt atcgttgccc acggtggtcg tggtggacgt ggaaatattc gtttcgcgac
    15421 accaaaaaat cctgcaccgg aaatctctga aaatggagaa ccaggtcagg aacgtgagtt
    15481 acaattggaa ctaaaaatct tggcagatgt cggtttagta ggattcccat ctgtagggaa
    15541 gtcaacactt ttaagtgtta ttacctcagc taagcctaaa attggtgcct accactttac
    15601 cactattgta ccaaatttag gtatggttcg cacccaatca ggtgaatcct ttgcagtagc
    15661 cgacttgcca ggtttgattg aaggggctag tcaaggtgtt ggtttgggaa ctcagttcct
    15721 ccgtcacatc gagcgtacac gtgttatcct tcacatcatt gatatgtcag ctagcgaagg
    15781 ccgtgatcca tatgaggatt acctagctat caataaagag ctggagtctt acaatcttcg
    15841 cctcatggag cgtccacaga ttattgtagc taataagatg gacatgcctg agagtcagga
    15901 aaatcttgaa gaatttaaga aaaaattgtc tgaaaattat gatgaatttg aagagttacc
    15961 agctatcttc ccaatttctg gattgaccaa gcaaggtctg gcaacacttt tagatgctac
    16021 agctgaattg ttagacaaga caccagaatt tttgctctac gacgagtccg atatggaaga
    16081 agaagcttac tatggatttg acgaagaaga aaaagccttt gaaattagtc gtgatgacga
    16141 tgcgacatgg gtactttctg gtgaaaaact catgaaactc tttaatatga ccaactttga
    16201 tcgtgatgaa tctgtcatga aatttgcccg tcagcttcgt ggtatggggg gtgatgaagc
    16261 ccttcgtgcg cgtggagcta aagatgggga tttggtccgc attggtaaat ttgagtttga
    16321 atttgtagac taggagactg gtatgggaga taaaccgata tctttccgag atgcggatgg
    16381 taattttgtt tccgccgcag acgtttggaa tgaaaagaaa ttggaagaac tatttaatcg
    16441 tctcaatcca aatcgtgcct tgagattggc acgaactaaa aaggaaaatc catctcagta
    16501 aagaagctaa aaaatcccgt gcctcatcag acacgggatt ttgtggtacg acaggcatgt
    16561 atagcaaact gaatctggaa tagcacagca tatcttctaa aatattgcta taaattgatt
    16621 tgaatttcaa aattaaattg ttttattctt atttcaattt gttatagtat atctgatgtc
    16681 aaagttctcg gcgagtcaaa tagtgattcc caagcctgac tatcgtgagg tagcggatta
    16741 aaatggtctg gggatagacc gttttaagtc tgatgctgga aataagaatt gtcagaagaa
    16801 gggatagcga aatcgtggct ctacgaacag gaacgtgata ataaggcgta tatagcgaat
    16861 aagagggcct caaactctaa agtccaaaaa ggtagtcgta acctatatgc gtaaatcatg
    16921 agagtaattg aattcgtact aagattttct atttttactg taacctttta acgtcctcat
    16981 atcttgtata cacgaggaaa gatgtacgac ttatcccgtg aggtctatca ctataaagag
    17041 aaaacgacag atagaagtga tcctgagtca cggttatctt taaaatagat tgtctgatag
    17101 gatgctagcc tgaaaacgtc ttgttcatga aatataggac ggtatgtata aaacgcttct
    17161 gtgaactgag agaaggggga gaagttcttg ctaaaattta gttgaacagc cgtattccga
    17221 tacttagata agagatctag tcttagctcc tactcagttt caggggataa aaaaggggca
    17281 atagcgattc gagaaagatt atactcttcg aaaatctctt caaatcacgt caatatcgcc
    17341 ttgccgtatg tgtaggatac taactacgtc agttccatct acaatctcaa aacagtgttt
    17401 tgagcaacct gcggctagtt tcctagtttg atctttgatt tttattgagt attagtaatt
    17461 cagttactaa ctcgtcaact ctgatttatc caataaaatt gaaaaggatg gaaaaaagga
    17521 taaatttatg atatacttta ttttgaagac cttattagaa atcttgaaag agtattgaaa
    17581 acttagaatg agaaaaattg ttatcaatgg tggattacca ctgcaaggtg aaattactat
    17641 tagtggtgct aaaaatagtg ttgtggcctt aattccagct attatattgg ctgatgatgt
    17701 ggtgactttg gattgtgttc cagatatttc ggatgtagcc agtcttgtcg aaatcatgga
    17761 attaatggga gctactgtta agcgttatga cgatgtcttg gagattgatc caagaggtgt
    17821 tcaaaatatt ccaatgcctt atggtaaaat taacagtctt cgtgcatctt actattttta
    17881 tgggagcctc ttaggccgtt ttggtgaagc gacagttggt ctaccgggag gatgtgatct
    17941 tggtcctcgt ccgattgact tacaccttaa ggcgtttgaa gctatgggtg ccactgctag
    18001 ctacgaggga gataacatga agttatctgc taaagataca ggacttcatg gtgcaagtat
    18061 ttacatggat acggttagtg tgggagcaac gattaatacg atgattgctg cggttaaagc
    18121 aaatggtcgt actattattg aaaatgcagc ccgtgaacct gagattattg atgtagctac
    18181 tctcttgaat aatatgggtg cccatatccg tggggcagga actaatatca tcattattga
    18241 tggtgttgaa agattacatg ggacacgtca tcaggtgatt ccagaccgca ttgaagctgg
    18301 aacatatata tctttagctg ctgcagttgg taaaggaatt cgtataaata atgttcttta
    18361 cgaacacctg gaagggttta ttgctaagtt ggaagaaata ggagtgagaa tgactgtatc
    18421 tgaagacagc atttttgtcg aggaacagtc taatttgaaa gcaatcaata ttaagacagc
    18481 tccttaccca ggctttgcaa ctgatttgca acaaccgctt acccctcttt tactaagagc
    18541 gaatggtcgt ggtacaattg tcgatacgat ttacgaaaaa cgtgtaaatc atgtttttga
    18601 actagcaaag atggatgcgg atatttcgac aacaaatggt catattttgt acacgggtgg
    18661 acgtgattta cgtggggcca gtgttaaagc gaccgactta agagctgggg ctgcactagt
    18721 cattgctggg cttatggctg aaggtaaaac tgaaattacc aatatcgagt ttatcttacg
    18781 tggttattct gatattatcg aaaaattacg taatttagga gcggatatta gacttgttga
    18841 ggattaaacc gtagaggtgt ttatgaatat ttggaccaaa ttagcaatgt tttctttttt
    18901 tgaaacggat cgcttgtatt tgcgtccttt cttttttagt gatagtcagg acttccgcga
    18961 gatagcttca aatccagaaa atcttcaatt tattttccca acgcaggcaa gtctggaaga
    19021 aagtcaatat gcactggcca attactttat gaagtcccct ttgggagtgt gggcaatttg
    19081 tgaccagaaa aatcaacaaa tgattggttc tattaaattt gagaagttag atgaaatcaa
    19141 aaaagaagct gagcttggct attttttgag aaaagatgct tggtcgcaag gatttatgac
    19201 agaggttgtt agaaaaattt gtcagctttc ttttgaggaa tttggcttaa aacaattatt
    19261 tatcattacc caccttgaaa atgaagctag ccaaagagtt gctcttaagt ctggatttag
    19321 tttgttccgt cagtttaagg gaagtgatcg ttacacaaga aaaatgcggg attatcttga
    19381 atttcggtat gtaaaaggag agttcaatga gtaagcatca ggaaattcta agctatttgg
    19441 aggaattacc agtaggtaaa agggtcagtg ttcgtagcat ttcgaatcat ctaggagtta
    19501 gtgatggaac agcctatcga gctattaaag aagctgaaaa ccgtggaatt gtggagaccc
    19561 gtcctagaag tggaacaatt cgtgttaaat cccagaaagt tgctatagag agattaacgt
    19621 ttgctgaaat tgcagaagtg acttcttctg aggttctggc tgggcaagaa ggtttagaga
    19681 gagagtttag taagttttca attggtgcca tgactgaaca aaatatcttg tcttaccttc
    19741 atgatggggg gctcttgatt gtcggagacc gaacccgtat tcagttgcta gccttggaaa
    19801 atgaaaatgc agttctggtt acagggggat ttcaggttca tgatgatgtg cttaaactgg
    19861 ccaatcaaaa agggattcct gttctaagaa gtaagcatga tacctttacc gtcgcgacca
    19921 tgatcaataa agccttgtca aatgtccaaa tcaagactga tattctgaca gttgagaaac
    19981 tttatcgccc tagtcatgag tatggttttc tgagagagac agatacagtt aaagattatt
    20041 tggacttggt tcgtaagaat cgtagcagcc gtttccctgt tatcaatcaa catcaggtcg
    20101 ttgttggcgt tgtaaccatg agagacgctg gtgataaatc accaagcacg acaattgata
    20161 aggttatgtc tcgtagtcta tttttggttg ggttatcgac aaatattgcc aatgtgagtc
    20221 aacggatgat cgcagaagac tttgaaatgg taccagttgt tcgaagcaat caaactttgc
    20281 ttggcgttgt gacgcgacga gatgtcatgg agaagatgag ccgttcccaa gtttcggctc
    20341 taccaacttt ttctgagcag attggacaaa agctctctta tcaccatgat gaagtagtca
    20401 ttacagtgga accctttatg ctagaaaaaa atggagtttt ggctaatggt gtattggcag
    20461 aaattctgac ccacatgacc caagatttag ttgttaatag tggtcgcaat ctcattatcg
    20521 agcagatgct gatctacttt ttgcaggctg ttcagataga tgatatattg cgcattcagg
    20581 cacggattat tcatcatacg agacggtcag ctataattga ttacgatatt tatcatggtc
    20641 accagattgt ttcaaaagca aatgtgactg ttaaaattaa ttagaaacta ggagaaaaga
    20701 tgataacatt aaaatcagct cgtgaaatcg aagctatgga caaggctggt gattttctag
    20761 caagtattca tataggctta cgtgatttga ttaagccagg cgtagatatg tgggaagttg
    20821 aagaatatgt ccgccgtcgt tgtaaagaag aaaatttcct tccacttcag attggggttg
    20881 acggtgccat gatggactat ccttatgcta cctgttgctc tcttaacgat gaagtggctc
    20941 acgctttccc tcgtcattat atcttgaaag atggtgattt gctcaaagtt gatatggttt
    21001 tgggaggtcc cattgctaaa tctgacctga atgtctcaaa attaaacttc aacaatgttg
    21061 aacaaatgaa aaaatacact cagagctatt ctggtggttt agcagactca tgttgggctt
    21121 atgctgttgg tacaccgtcc gaagaagtca aaaacttgat ggatgtaacc aaagaagcta
    21181 tgtacaaggg tattgagcaa gctgttgttg gaaatcgtat cggtgatatc ggtgcggcta
    21241 ttcaagaata cgctgaaagt cgtggttacg gtgtagtgcg tgatttggtt ggtcatggtg
    21301 ttggcccaac tatgcacgaa gaaccaatgg ttcctaacta tggtattgca ggtcgtggac
    21361 tccgtcttcg tgaaggaatg gtcttaacca ttgaaccaat gatcaataca ggcgattggg
    21421 aaattgatac agatatgaaa actggttggg cgcataagac cattgacggt ggattgtcat
    21481 gtcagtatga acaccaattt gtcattacga aagatggacc tgttatcttg actagccaag
    21541 gtgaagaagg aacttattaa taaaaagtga aaagactact ggaagtttat tttgataaaa
    21601 aatccagtag atcttttcat aataaaacgc attgtatcaa gtgttaggag ctgatatcat
    21661 gcgtttttct gcttttaaga ttttttccaa ctctgtttgt aagcgcatca taacaaaggg
    21721 tctaggattc agggctctcc tcctatatac tattagtaaa gtaaaactaa gggaggatat
    21781 tttagtgtcg cagtctattg ttcctgtaga gattccacaa tattgtcgtt ttgattctaa
    21841 aaagagaaat ggaattctgt ttaacgttcg tattgccaat cttaaattta ccttttttag
    21901 attagactcc ctgcgaaaca aaatatggta tagtagttct atgaatgatg aagcaagtaa
    21961 acaactaact gatgcacgat ttaagcgtct tgttggtgtt cagcgcacga cttttgaaga
    22021 gatattagct gtattaaaaa cagcttatca acttaaacac gcaaaaggtg gacgaaaacc
    22081 taaattaagc ctagaagacc ttcttatggc cactcttcac tatgtgcgag aatatcgaac
    22141 ttatgaagaa attgcggctg attttggtat ccacgaaagc aacttactcc gtcggagcca
    22201 atgggttgaa gtaactcttg ttcaaaggtg gtgttacgat ttcaagaact cctctcagct
    22261 ctgaggacac ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct aaaaaagaat
    22321 tagcgaatca ttctggtaaa aagaaatttc acgctatgaa ggctcaagcg attgtcacaa
    22381 gtcaagggag aattgtttct attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa
    22441 tattggacaa gctggtaaaa tcttggctga cagtggttat caagggctca tgaagatata
    22501 tcctcaagca caaactccac gtaaatccag caaactcaag ccactaatag ctgaagataa
    22561 agcttataac catgcgctat ctaaggagag aagcaaggtt gagaacatct ttgccaaagt
    22621 aaaaacgttt aaaatgtttt caacaaccta tcgaagtcat cgtaaacgct tcggattacg
    22681 aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc
    22741 tattgaggta ttgagctagt ttatgaaaaa attgggtgaa aagtcgagtg ttttagaaac
    22801 ccacagtgta gtattctagt ttcaatccac tatattttgc tactccccgt aaagtttcta
    22861 ttttccctga tttctgatat aatagaaata ttgacttcaa gagtaaggaa gagaagatga
    22921 acgcattatt aaatggaatg aatgaccgtc aggctgaggc ggtgcaaacg acagaaggtc
    22981 ccttgctaat catggcaggg gctggttctg gaaagactcg tgttttaacc caccgtatcg
    23041 cttatttgat tgatgaaaag ctggtcaatc cttggaatat cttggccatt acctttacca
    23101 acaaggctgc gcgtgagatg aaagagcgtg cttatagcct caatccagct actcaggact
    23161 gtctgattgc gaccttccac tccatgtgtg tgcgtatttt gcgtcgcgat gcggaccata
    23221 ttggctacaa tcgtaatttt acaattgtgg atcctggtga acagcgaacg ctcatgaaac
    23281 gtattctcaa acagttgaac ttggatccta aaaaatggaa tgaacgaact attttgggga
    23341 ccatttccaa tgctaagaat aatttgattg atgatgttgc ttatgctgcc caagctggcg
    23401 atatgtatac gcaaattgtg gcccagtgtt atacagccta tcaaaaagaa cttcgtcagt
    23461 ctgaatccgt tgactttgat gatttgatta tgctgacctt gcgtctcttt gatcaaaatc
    23521 ctgatgtttt gacctactac cagcaaaaat tccaatacat ccacgttgat gagtaccaag
    23581 ataccaacca cgctcagtac caattggtca aactcttggc ttcccgtttt aaaaatatct
    23641 gtgtggttgg ggatgcggac cagtctatct acggttggcg tggtgctgat atgcagaata
    23701 tcttggactt tgaaaaggat taccccaaag ccaaggttgt cttgctggag gaaaattacc
    23761 gctcaaccaa aaccattctc caagcggcca acgaggttat taaaaataat aaaaatcgcc
    23821 gtcctaaaaa tctctggact caaaacgctg atggggagca aatcgtttac tatcgtgccg
    23881 atgatgagct ggatgaggct gtatttgtag ccagaaccat cgatgaactt agtcgcagtc
    23941 aaaacttcct tcataaggat tttgcagttc tctatcggac taatgcccag tcccgtacaa
    24001 ttgaggaagc cctgctcaag tctaacattc cttataccat ggttggcgga accaaattct
    24061 acagccgtaa ggaaattcgc gatattattg cttatctcaa ccttattgct aatttgagtg
    24121 acaatattag ttttgagcgt attatcaacg agcctaaacg tggaattggt ctaggtacag
    24181 ttgagaaaat ccgtgatttt gcaaatttgc aaaatatgtc tatgctggat gcttctgcta
    24241 atattatgtt gtctggtatc aagggtaagg cagcccaatc tatctgggat tttgccaata
    24301 tgatgcttga tttgcgggag cagctagacc acttaagcat tacagagttg gttgagtccg
    24361 tcctagaaaa aacaggttat gtcgatattc ttaacgccca agcgactcta gaaagcaagg
    24421 cacgggttga aaatatcgaa gagtttcttt ctgttacgaa gaactttgat gacaccacgg
    24481 atgtgacaga agaggaaact ggtctggaca aactgagtcg tttcttaaat gacttggctt
    24541 tgattgccga cacagattca ggtagtcagg agacatcaga agtgaccttg atgaccctgc
    24601 atgctgccaa aggtctcgaa tttccagttg tctttttgat tgggatggaa gaaaatgtct
    24661 ttccacttag tcgtgcgact gaagatccag atgaattaga agaagagcgc cgtctagcct
    24721 atgtaggtat cacgcgtgca gagaaaattc tctatctgac caatgccaac tcacgcttgc
    24781 tttttggtcg taccaattat aaccgtccga ctcgttttat taacgaaatc agttcagact
    24841 tgcttgagta tcaaggtctg gctcgtcctg caaatacaag ctttaaggca tcatatagca
    24901 gtggtagtat ttcctttggt caaggtatga gtttggctca ggctcttcaa gaccgtaaac
    24961 gcggtgctgc cccaaaatca atccagtcaa gcggtcttcc atttggtcaa tttacagctg
    25021 gcgcaaaacc agcatctagc gaggcaaatt ggtccattgg tgatattgct ctccacaaga
    25081 aatggggaga gggaaccgtt ctggaagttt caggtagcgg tgctaggcag gaattgaaaa
    25141 tcaatttccc agaagtaggt ttgaaaaaac ttttagccag tgtggctcca attgagaaaa
    25201 aaatctaatt ttccatcctt ctcacgaata ataaagtgag gaggattttt atgtacagta
    25261 tttcattcca agaagattca ctattaccaa gagaaaggct ggccaaggaa ggagttgaag
    25321 cgcttagtaa ccaagagttg ctagctattt tactcaggac aggaacacgt caagttagcg
    25381 tttttgaaat tgcccaaaaa gtcttgaaca atctttcaag cctaacggat ttgaaaaaaa
    25441 tgaccctgca ggaattgcag agtttgtctg gtattgggcg tgttaaggcc atagaattac
    25501 aagctatgat tgaactgggg catcgtattc acaaacacga gactcttgaa atggaaagta
    25561 ttctcagcag tcaaaagttg gccaagaaga tgcagcagga attaggggat aaaaaacaag
    25621 agcacctggt ggcactctat ctcaatactc aaaatcaaat catccatcag cagaccattt
    25681 ttatcgggtc tgtaactcgt agtatcgctg aaccgcgaga gattcttcac tatgcaatca
    25741 agcatatggc gacttctctt atcttggtcc acaatcatcc ttcaggagcg gtagcgccta
    25801 gccaaaatga tgatcatgtc actaaacttg ttaaagaagc ctgcgaattg atggggattg
    25861 ttctcttgga ccatttgatt gtctctcatt ctaattactt tagttatcgt gaaaagacag
    25921 atttaatcta aagttcatta acgacatagt caaagagttt tttatctttg ggacgatttt
    25981 caaaaagaag ttctggatgc cattggacac cgagaaaggc gacatcatcc gtactcatga
    26041 cagcctcaat gataccatct ttaggatcat gagccacaac ttttaaattt ggtgctaagt
    26101 ccttgatgct ctggtggtgg aaggagttga tatgagagat ttctccatag atttcttgga
    26161 gaacggtatc tggttctgtt accaagcgtt gagttgtgta ctcaacagaa gaatcctgcc
    26221 aatggtcttc gatatcttgg tacaaagttc cacccatggc aacgttaaag agttgggtac
    26281 cacggcagac agagaaaatg ggctttttct gtttaatagc ttccttgatg agggtcagtt
    26341 cgaagatatc tctttgaagg tgatagtcat cgctatcaat ggttttgggt tcgccataaa
    26401 attttggatc gacattttgc ccacctgtca agatgagctt gtcaatcaaa ctgatatagt
    26461 ggcaggccat ttcttgatca ccaatcggta ggatgatggg aatccctcca gcatctttaa
    26521 cgccttcaac aaagcctttt gctgcgtagc tcatcatgat gtcatcatct ggatgagctt
    26581 tttcgtttcc tgtaatccca ataactggtt ttttcataaa atgattttcg ctttctaatc
    26641 ctcttttcgc atgaagtaga ggagggtttg gagttcactt gtcaaatcga catactgaac
    26701 gaccacgtct tttggtaaat gcagatggac tggtgaaaaa ctgagaattc ctttcacacc
    26761 agcatcaacc aagagattag caacctcttg tgacttgacg ctgggaacag ttaggatagc
    26821 agtcttcaca tcagcatcct tgattttatc cttgatctga gaaatcccgt aaatgggaat
    26881 cccgtcagga gtttgggtac cgacttcagg atggtcgtct aggtcaaagg ccatgataat
    26941 cttcatcttg ttacgttcgt ggaagcggta gtggagaagg gcatggccca tatttccaat
    27001 accaaccagc atgacattgg taatagagtt gtcattgagc aaatcggcaa aaaatgtcat
    27061 tagttttttg acatcatagc caaaaccacg acgaccaagt tcaccaaaat aggaaaaatc
    27121 acgacgtacg gtcgctgaat caataccgat agcctctgca atttgcttag agttggcacg
    27181 ttcaatcttt tctgcatgaa atctcttaaa aattcgatag tagagagaga gtctttttgc
    27241 tgtagctttt ggaatagcaa actgtttatc tttcacaaaa t
//
LOCUS       DAVLWA010000019        26207 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLWA010000019 DAVLWA010000000
VERSION     DAVLWA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.019"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            108..1323
                     /gene="sstT"
                     /locus_tag="VYN80_000804"
                     /pseudo
     CDS             108..1323
                     /gene="sstT"
                     /locus_tag="VYN80_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            1391..2344
                     /locus_tag="VYN80_000805"
     CDS             1391..2344
                     /locus_tag="VYN80_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1540747.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(2442..2519)
                     /locus_tag="VYN80_000806"
                     /pseudo
     CDS             complement(2442..2519)
                     /locus_tag="VYN80_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2580..2804)
                     /locus_tag="VYN80_000807"
     CDS             complement(2580..2804)
                     /locus_tag="VYN80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEV1540748.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(2809..2988)
                     /gene="asp4"
                     /locus_tag="VYN80_000808"
     CDS             complement(2809..2988)
                     /gene="asp4"
                     /locus_tag="VYN80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEV1540749.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(2990..4327)
                     /gene="gtfB"
                     /locus_tag="VYN80_000809"
     CDS             complement(2990..4327)
                     /gene="gtfB"
                     /locus_tag="VYN80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEV1540750.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(4320..5831)
                     /gene="gtfA"
                     /locus_tag="VYN80_000810"
     CDS             complement(4320..5831)
                     /gene="gtfA"
                     /locus_tag="VYN80_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEV1540751.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(5843..8215)
                     /gene="secA2"
                     /locus_tag="VYN80_000811"
     CDS             complement(5843..8215)
                     /gene="secA2"
                     /locus_tag="VYN80_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEV1540752.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(8208..8648)
                     /gene="asp3"
                     /locus_tag="VYN80_000812"
     CDS             complement(8208..8648)
                     /gene="asp3"
                     /locus_tag="VYN80_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEV1540753.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(8645..10180)
                     /gene="asp2"
                     /locus_tag="VYN80_000813"
     CDS             complement(8645..10180)
                     /gene="asp2"
                     /locus_tag="VYN80_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV1540754.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(10164..11744)
                     /gene="asp1"
                     /locus_tag="VYN80_000814"
     CDS             complement(10164..11744)
                     /gene="asp1"
                     /locus_tag="VYN80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEV1540755.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(11754..12971)
                     /gene="secY2"
                     /locus_tag="VYN80_000815"
     CDS             complement(11754..12971)
                     /gene="secY2"
                     /locus_tag="VYN80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEV1540756.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(13028..13705)
                     /locus_tag="VYN80_000816"
     CDS             complement(13028..13705)
                     /locus_tag="VYN80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1540757.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(13690..13934)
                     /locus_tag="VYN80_000817"
                     /pseudo
     CDS             complement(13690..13934)
                     /locus_tag="VYN80_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(13934..15130)
                     /locus_tag="VYN80_000818"
     CDS             complement(13934..15130)
                     /locus_tag="VYN80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1540758.1"
                     /translation="MRKSIVLAADNVYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNSGVLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(15139..16359)
                     /locus_tag="VYN80_000819"
     CDS             complement(15139..16359)
                     /locus_tag="VYN80_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV1540759.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(16384..17268)
                     /locus_tag="VYN80_000820"
     CDS             complement(16384..17268)
                     /locus_tag="VYN80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1540760.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDDWVTEEYLSI
                     LIETLKEQHSEIVVSTYSTYNESDGLFYIHVFDSDYYVKNYNSKLLMEELPLLERYDM
                     SFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYKLFMQVEKVTYINKVLYWYRVGKEG
                     LSNSYSPKMMRDDCDFRLERIAVLALKGYDVSKYLDQMKFYLKYRHDIAIQRELKENV
                     ETRHLEMLDYLLNGNKYN"
     gene            complement(17265..19718)
                     /locus_tag="VYN80_000821"
                     /pseudo
     CDS             complement(17265..19718)
                     /locus_tag="VYN80_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
     gene            complement(19728..20738)
                     /locus_tag="VYN80_000822"
     CDS             complement(19728..20738)
                     /locus_tag="VYN80_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1540761.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKRTFFAGSLERFPDLQNWSQDTPLRVFSNKGEAGSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(20767..21357)
                     /locus_tag="VYN80_000823"
     CDS             complement(20767..21357)
                     /locus_tag="VYN80_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEV1540762.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVVAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(21372..21644)
                     /locus_tag="VYN80_000824"
     CDS             complement(21372..21644)
                     /locus_tag="VYN80_000824"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1540763.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGRTLLL"
     gene            complement(21649..22863)
                     /locus_tag="VYN80_000825"
     CDS             complement(21649..22863)
                     /locus_tag="VYN80_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1540764.1"
                     /translation="MYRKVIALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSE
                     WFLLMRNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYL
                     DSDIIVNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKSTQN
                     LLELTAQHHEHVYGDQGVLNIYFGDQWLHLDKEYNFMVGLDQFLHLKGNADWYQSNYY
                     GNYEPKIVHYTTEFKPWTHLTLTRFRKLWWFYYGLNWNDVLLSSDIVKRSFKELVGVP
                     LYHTCIFTNSAAMESLEYLLSELPEVHFTILAHTNFAPSVVDLQRYLNLSLFPNFNSF
                     NMKETLDKMDFYLDINHEGEIVNIIEEVCRRNKPIFSFDNTSHDSKGRSRIFSATEPD
                     KMVDAIRKFLGE"
     gene            complement(23220..24098)
                     /locus_tag="VYN80_000826"
     CDS             complement(23220..24098)
                     /locus_tag="VYN80_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV1540765.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNNGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKMRV"
     gene            complement(24095..25495)
                     /locus_tag="VYN80_000827"
     CDS             complement(24095..25495)
                     /locus_tag="VYN80_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1540766.1"
                     /translation="MHALVDAMSERITLLANMGYPLEKHLAIYRQMLEFSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIRSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            complement(25542..>26207)
                     /locus_tag="VYN80_000828"
     CDS             complement(25542..>26207)
                     /locus_tag="VYN80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1540767.1"
                     /translation="SASASTSASVSASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASAST
                     SASASASTSASESASTSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SASASESASTSASASTSAGKSRQQLPNTGTEASKSSVLLGALAAVTGLGLFAKRRKRD
                     DEE"
BASE COUNT         8249 a         5668 c         4144 g         8146 t
ORIGIN      
        1 ttcatcagtc tcattctcca taaatattca aaaaatagac agaaaattct tgattttgtc
       61 tatcatttat actataatca taatcactac acaaaaagga gattattatg aaaagaatca
      121 ttcatgcttg gaataaggca agccttgtca agcgtatctt gattggtatg ctttgtgggg
      181 aatcctagga ctgacccttc ctaatctctc agggattggg ctactcgagg atttattcgt
      241 tggaggtcta aaagctgttg ctcctatcct agtctttgct ctcgttgcca atgccctttc
      301 ccaacatcaa aagggacaag atagcaatat gaaaactgtt gttttcttgt atatcctatg
      361 atagggactt tcgccgctgc tcttgtagct gtactagcaa gtttcatcgt ccctattgaa
      421 attaccctaa atagtgccaa tactgaaatt gcaccaccag atgggattgg gcaggtcctc
      481 agcaacctct tgctcaaact ggttgacaac ccagtcaacg ccctgcttac tgctaactat
      541 attggaatct tatcttgggc agtcattttt ggaatcgcta tgagagaagc cagtaaaaat
      601 agtaaagaat tgctaaaaac tatcgctgac gtgacttcta aaattgtcga atggatcatc
      661 aatctggctc catttggaat ccttggtctt gtttttaaaa ccatttctga caagggagtc
      721 ggaagccttg ccaactacgg tattttattg gttccattag taacgactat gctttttgtt
      781 gcccctgtgg tcaacccttt gattgccttc ttctttatga gtatgagacg caatccttac
      841 cctctagttt ggaactgcct ccgtgtcagc ggtgtgacag cctttttcac tcgtagttct
      901 gcgactaaca ttcctgtcaa catgaaactc tgccatgacc ttggactcaa cccagatacc
      961 tattctgttt ctatcccact cggttctact atcaatatgg ctggagtagc gattaccatt
     1021 aacgttttga cccttgttac agttaacact cttggaattc ctgttgactt tgccacagcc
     1081 tttgtcctca gtgtggtagc agctatctca gcctgtggtg cttcaggtat tgccggaggt
     1141 tccctccttc ttatcccagt tgcttgtagc cttttcggta tttctaacga tattgccata
     1201 caagttgttg gggttggttt tgtgattggt gtcatccaag actcatgtga aacagccctt
     1261 aactcttcta cagatgtcct ctttaccgcc gttgccgaat acgcagcaac ccgtaaaaaa
     1321 taactcatca aggcaagcct gctcatgtct tgtcttttac gcttttattc taacttatta
     1381 ggaaattctt atgtctatta gccaacgtac gaccaagctc atcttagcta cctgtcttgc
     1441 ctgcctgctt gcttattttc tcaatctttc gtcagcagtt tcggctggaa ttatcgctct
     1501 cttgagccta tctgatacgc gtagaagtac tttaaaactg gctcgcaatc gtcttttttc
     1561 tatgcttcta gctctggcta tcggtgttct agcttttcac ttgagcggat ttcatatctg
     1621 gagtctcggc ctctatctgg ccttctacgt tcctttagcc tacaagatgg gctgggaaat
     1681 tggcatcaca ccaagcacgg ttttggttag ccatctcttg gttcaagagt caacctctcc
     1741 agacctccta gtcaacgaat tccttctctt tgctattggt acaggatttg ccttgcttgt
     1801 taatctctat atgccttcac gagaagagga aatccagcac taccacacgc tggtggaaga
     1861 aaagttaaaa gatatcctcc agcgcttcaa atactattta tccagaggag acggacgcaa
     1921 ccgagcacag ctggtagcag aattagacac gcttttgaaa gaagccctca gactggtcta
     1981 tttggatcac tctgaccacc tctttcacca gaccgactac catatccact actttgagat
     2041 gagacaacga caaagtcgta tcctgagaaa catggcccaa cagattaaca cttgtcacct
     2101 tgccgccagt gaaagcctga tcttagcgca actcttttca aaaattgcag gtcaactgag
     2161 ccagaccaat cctgcttctg atttgctaga tgaaattgaa cgttatctgg aagtcttccg
     2221 gaaccgcagt ctgcccaaga caagagaaga atttgaaacc cgcgccaccc ttcttcaact
     2281 cctacgtgaa gccaaaacct tcatccaagt aaaagttgat ttttaccaaa aatatagaca
     2341 gtaatactct tcaaactacg tcagcttcac cttgccgtat atatgttact gacttcgtca
     2401 gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagttct atctacaacc
     2461 tcaaaacggt gttttgagca gcctgcggct agcttcctag tttgctcttt gattttcatt
     2521 gagtataaaa aagaaaactt cagaccaatc cacaaaaggt gaatatctga agttttcttt
     2581 tatttataag taatcaccat aaaggttaat agtagaacaa ataaagccaa actcaccaat
     2641 aaagtgagca ccttgaccaa ggtgttgctc tgccagtagc ttggtttacc aatattacta
     2701 gtggcatcca tggaaaatag ttgattttga cggggttgta tagttaccaa tacaatcaaa
     2761 gcaagagata ggataatagc taaaacaatt agtatttcaa tcatagatct acctcaaaat
     2821 tcccagcaaa gtaaagagca aaccaatcag aatcatcaaa cccagtaaaa gtgaaaaagt
     2881 cttactaatc ttttcccctc gggtttcttt ttccttcttg atgggttttt gtttcaactc
     2941 ttccatgcgg ccttcaacgt ctttgtaaaa taaatcttcc tctgacatgt tagcctccta
     3001 aaacagtttg catggtttcc tgatatctca ccaagtcaac ataatttgca tgttggcctt
     3061 gtttgccaag tgcctgacgc atttgatcaa catctgaaag ggccaattta atggtctcaa
     3121 ccaaagcagc aacttcacta ctttcaaata gatggtctgg agcgatataa agtctattgt
     3181 gcaccgtctg attaaagcca agaatcaaga gattgtgctc aaaggcctga cgcactgcct
     3241 gtagtaactc attactgtgg tttatatcca agtaaatatc cgacagttga tacagctcct
     3301 gaatcttctg tggactagca ttctggtaaa ggaccacatt aggatagcga agcatgtcta
     3361 agagcttaga agacatctct gtcaccgctg caatacggaa agtgacatca ggcaaggctc
     3421 ctgcgattgc ttctacttgc tcaatctgat ctgaattggt caagattaag gcatctcgtc
     3481 ttaggaaatt atcacgtttg aactggtaat gataacccaa gtgcacaaac tgatcatggt
     3541 atttctcgtc agttaactct aaagcgcgct cataagtcgc cttatttgga atgatgatct
     3601 tcttagtacg cacattatca ctttccaaaa tcaactgcat attacctgga atggcatcat
     3661 agagaggttc ctgccatacc aagacatccg agccagattt atctggatga tggaaggaaa
     3721 ccaagaaagg agtcgctaga gtattaaaga taagctgact ggtatctatt tccaaatctt
     3781 gcaaaaagaa ggtgataaat tcaactttat ttgcaaagta acgcatggac tgaccaggca
     3841 aagtcaataa gatatcaccc gtcacatggt tttccagtaa aacttgttgg ccattgacat
     3901 cttggtaaac tgtcataatc ggctcgctat ctgcgctata agtggtttta gcaaagcaag
     3961 caccaaagcg attgtagtgg tcaacctgac gtactcgacc ttctaggtct ttccagtcta
     4021 cctgtttaac caagcgagcc tgcattccat caacataatg aatgacagcc ctctcctgcg
     4081 tcacatcttc aatacaagca gactgattat ctcctaaaat ttcccaaaaa tctgaaacgg
     4141 gaacttggtt aaaatagaga ggttttccat cctcgtaacc tagataatag gtaaaaggag
     4201 aaagcagacc atcaggcaga aaaccatctg catcgatgac cactccaagt tgagaaagac
     4261 cagtagcgac tagactttca tgtaaatctc gactttcatg actataacga tcataaagtt
     4321 caatcatgga gcacctcctc tactgttttc ttccactttt ctaaaatttc tttggtcaag
     4381 aagccttctg caatttggta agaataggca cgcatagctt ccaaacgatt ttcttgatac
     4441 aattgacaaa tcttagcggc ataagcttgc ttgatttggt cttctacatg gtcagatgaa
     4501 cttggaatca aataaccatt ttgcccatcc tctataaagg tctgattacc ataaggcaca
     4561 tcaaaaccaa ttagaggtag acctgaacca atagcttcca tcaaggtcag accaaatcct
     4621 tcgctggtag aagccgttaa gtagacctca tactggctat aaatctgcga aagttccgca
     4681 tgccccttga gttggatata gtcctctgcc tgatgatttg caataatgtc tctaagcaga
     4741 gaatcttctc caccactacc atagatatca aaggttagtt ccggtaactc cttatgagct
     4801 tcaatcacag ctttcacaag ccaatcaatg tgcttttctt tggcaagacg tgaagccgta
     4861 atcaatgaaa atggtttgcg cccttgactt gaatctgtca aggaatcaat actgcctaca
     4921 ggaatggtaa caatctttgg ctgatgctga gtatatttgg caaattgctc ttgtagaact
     4981 tcattttgtc tatcagtaga cacgataaag aagtcaacct tatctgcatt ggtaaactga
     5041 tagtcataat agttattcca aaggatgtag tcctcatttg tagcattttc actataatgc
     5101 tccgcatgaa caactaccgc taaatgtgct gtctgtgctt cctcaaacac aacctgacca
     5161 atgcctgtct ccctatcgag aatgaccaaa tcagacttat tcaaattcaa agatttcata
     5221 aaggcacgca caaaagcttg ctttccatag aaaatcttat ccttgaaatg ataaacttct
     5281 tccttccctt gattcatcaa gatatcatag actggagtcc cgtcttcatt ataaaaagtt
     5341 cgttggtata agactgcaac attgtccttg ggagcaaaat actcgctaca ataacgcgta
     5401 taagaaaagt aatccttccg aatcaggttt cccttaaaaa catactcggc atgttgaacc
     5461 aagtccttgt tctcatcaac caaataacag gttacaaact tatcttggtc aaaaaagaat
     5521 acacgtaaaa ccttgccatt tttttctctg tgactttctt caccaccaaa gtaagccaag
     5581 acatcatcca ctgtcacgct agtaggtgca attttgatat ctgtgaaatg attataaagc
     5641 cagataacct gattatcatc aaaaccaata ttggctgtta agtgctgaat attatcggct
     5701 aaaatcatat ctgtaaagat aaacttagag gacagattta atttccgaaa aacaccagca
     5761 cgataggctt gagcgtattc aacaccgcta ctagcccaac caattcctaa atttatattg
     5821 taaattgtca tattttctcc tcttatggaa aatgagtcac gatttcacct tttggagtga
     5881 cctcaaccag ccccatcagg agattacgca ccatatccgc acggatttgt tctttcatag
     5941 cttcaaagcc cgcgtaggct tcttgatagt actctacgat tggatttttc tgactagcag
     6001 attgaccacc gatagccatg gatagctgtt gtagatagtc tacctgctct acccagttgt
     6061 catcaatggc tttaagcagt gaaagtcgta aaaactgttc atataagtca tgttgattaa
     6121 gtaattcttt cttttcagaa agttctttat caatcacctg cttcataaag ctacgaactg
     6181 cagttttgtc agttacatct atataatctg gaacctcttt aacatgaaaa ctaatattgg
     6241 tcacaataaa gtgaaacaat aattcacgac tagcatagtg atcagccgct acctcttctg
     6301 tatatctctc aatgatatcc acaacaacat cctctaagtc acgagaacca tctattagac
     6361 gatttctctc tttatagact atatcccgtt gaatattcat actttcagca tattctagag
     6421 tctgacgacg tgccgaacgg ccagcactat cactggcatg ctgagccttt tcgactagtt
     6481 tccggtattt acgacctttc aatacttccg gttgagtcat atcttgaacc tgatagtctt
     6541 tgtacttttt atgcacccaa gatggaccaa atttcttgat aacatcatcc tctaaggata
     6601 caaaaaattt actcatacca ggatctccct gacgaccaga acggccacga atttgtaggt
     6661 cgatccgctg actttccatc cgctcagtcc caataacaat caagccccca agctctgcga
     6721 ctcctttacc aagcttgata tccgtaccac gtcctgccat agaggtagcc actgtcacag
     6781 cccccatctg acctgactcg gagataatct gagcctcacg cgccgcatta ttagcattta
     6841 ggacattatg ggcaatccct tcacgaaaca agagagacga atagagttga gacatttcaa
     6901 ctgagcctac aaaaacgagt aagggatttc ccttagcatg gtattgcttg atgtactcca
     6961 aggatgcata cactttttca ggtaaagtga tatatagatt atctggatag tcaatccgtt
     7021 gtctcggacg attggttgga atgcgtacta cagacatatt gtaagtttca ataaactctt
     7081 tttccgcgac cttacctgtc cctgtcatac cagatatctt attaaacatc ttaaaaagac
     7141 tctgataggt gatcgaggcc atagcccgcg tctcaggaga taatttgaca tgttccttgg
     7201 cttcaatagc ctgatggaga cctccttgaa gtttagtcat ttccattaga cgccctgttc
     7261 ccttatcaac cagtaccatc tcatttccac gaatgatata gtccttatct ttagtaaaga
     7321 gcttatgagc tcgaatcgca taaaccaaat gacgagcaaa agacgcatgc tcttccttgt
     7381 ataaattatc aatccctagg aaattctcag cagacttggc ccccttagta gtgagccaaa
     7441 cctcctcttt ctcctcttta aagatataat cctctccttc gaccaaggtt gttacaagtg
     7501 tatcaatgat cgcatagtaa ttagactgaa cacgaggaga acccgcaata atcagaggag
     7561 tttgtgcact atcaagcaag atatcatcaa tttcatcaat aatcacatag ttaaacggtc
     7621 gtaaaaactt accttcttca ttcgaggcta ggttatcatt tagataatca aaacctaaat
     7681 tactattggt tgtgtagatg atatccgaag catagataag cttcttttct tcagctttca
     7741 tctccttctt tggatcttcc gtaaatggta caccaatggt caatcctaga aaacgataaa
     7801 cttgacccat ttcctcggca tcacgctttg ataaatactc attaggagtc acaaccatca
     7861 ctccttctcc tgaaaaagcg ttcaaataga caggcatggt agctgtcaag gtcttacctt
     7921 cccccgtatt catctcagca acatttccat agtgcatgac aatagctccc atgacttgaa
     7981 catcataagg aaacatccct aaaatccgct tatctgcttc acgcaccaca gcaaaagctt
     8041 caaccaaaat atcgtctaga ctttctccct cggaaagacg ctgacgaaac tctactgttt
     8101 tagctactaa ttcttgatcc gaaagagagg acatcttgcc tttcagggca ttaatctgct
     8161 ttaaaatctt tttcactttc ctaagctgga aatcttgacc taaacgtcta aacacttctg
     8221 atctccttga tagaaaaatg ataaaaagtt aaggactcaa atccagcact taaaagagaa
     8281 acttgataag tataactatc ttctggataa gtaaatatga aatctttcac tttttctacc
     8341 tttttttcta aaatatcacc atagcggtca aaaaaagtta tcttggtata aactccattt
     8401 tcaggctgac aatcaaaaac cagtgatagt tgatactcct gctttctttt taataaggga
     8461 agctgaggtt gggttcgtgc agcctggtaa ttgacactag aaaaccaagt ctttagaatc
     8521 tcaccagatg gcaatagagg attatacaga cgaacacttt tgtcagggta ataagataca
     8581 gttgttccat acatataagt cccaccaact tctccccaat gaatagactg tctctgtgta
     8641 ataatcatct tttctccctt ccaaaaccag tctctaagac cattctataa aagtgcaaaa
     8701 accaagaaat attggtatct gtatcatcat tatgtcgtcc agcagttcct ttagataaaa
     8761 tcttcgcacc tgtattacac agataagaca ctaattgttc ataggcttgt ggatccattt
     8821 cttcatcttt catataggat aaaccaaagg ttgtctttga aaaatttgct tgtttaaaaa
     8881 ttttccaaaa acgctgatcc aactccgtca tatcttgaga acttgtccca cctgtatgat
     8941 gaattaaaca atcaaaagct aagttagaga cacccggtgc gtccaaacgt ccacgactag
     9001 ctatagttcc taaattagcc aagggcttac caactatgat ggcatggggt tcaaaagaag
     9061 caccataata gagagccgga aacgttccca tagaaagacc tgataaaatc aaatccttat
     9121 gatcaaagcc taaataatca agatagtgag tgatcgtatc ctttactttt ccctctagct
     9181 catccgttcc gagataaaaa gctcccccct ctaaacgtgg atcagaaaat aaaatgaagg
     9241 gacatccaag agtcttcatc ataaagtaac cctcgaatcc ttctgcaggt cgataacctg
     9301 caaaatagac agtcaaaggc ggtttaaaat cacctggatg gaagaaatag tttatttcat
     9361 cacgcttgga atcatgtagg atatttccac caagtacaaa cttaccaaat tgttttcgac
     9421 tccatcgttg gtgaagatta cctagtttga tagttccacg acctcttgct tcaacagata
     9481 tcgagagaaa gtagtctccg tctcgactat ccataaaaag tgcttgctcc aagtcttttt
     9541 cagatactat tacttcttca aagaaatcat caacagaccc actccacatt ttacggatta
     9601 ctaaacgaaa gtcacaattg ccttccttct catattcaag ccacaactca atagggagtg
     9661 ttttttgcac cataatatta taagcccaat aggcaagctg ggcaaaatct tgcccaaact
     9721 caccttccaa actgacataa tccaatcctt gataagaaat agctcctgta aaatttggat
     9781 tgacttgtat cgtcggagga aagagtttat caccataccc accgccaaaa agagatttac
     9841 ttaaatcatt gattagttgt tggggactta aaaaatcaat tgcttgtgca catcgttttt
     9901 ttagaaaatc cacaacctct ctatcattta tttgtagatt ctggttataa aataaagtat
     9961 aaggttcaaa atattttaca taggaaaata agtctctgga atactgacca tcttctatta
    10021 aaactgcatg aaaacggtta atatcctctt tttcaattat ttcacgaagg gcttttgatg
    10081 agtttgggta aaaataatac caactcatat tttcaggtat gtcatagtga ttttcccaat
    10141 tggcaagtcc tatctgtaaa atattaagtt cctttgacat ttttaacctc ctctatccat
    10201 ttctctaatt ttccaagaaa ttgttgtcct gtgtgctcct taatcttatc aattgaatat
    10261 atcaaggact cattccactc cttcaaccta tctgtgtaat aatgtgcagc cttagaaaat
    10321 tctgtaacat ctgctaacaa ataaccattt tttaaatgtt caacatagac ggtttcaact
    10381 aggttgattt gaggaattcc tgcactaatc ccagcaatct gtgtgtagag atgaggatgt
    10441 ctatttaaat ccacaatcaa acggacaaat tctagtgttt ttatcaaatc taactcatca
    10501 ttcaagttaa caaactgtaa gcgtaagtcc tggtgttgat tttcttccag aggattttct
    10561 gcatcaccat aatcaatcgc ctttcccaga ttttcggatt gaatgttttc ttggatgaaa
    10621 gactcaacaa tcccttcaac ctcattcatt tgctcctgac tagcagcaaa ggctccaaaa
    10681 atcacctcag tatccttatt ttcagcgaca aaggacaaga cttgaagcag agcttgatta
    10741 tcaatcccct gctcaaaatc cagttgaaaa taaatgatgg attctttctt agtttggctt
    10801 cgtcccaatc gtaatcgggt gtcaaaagat gataaatgat aacattgatg cattcgttca
    10861 ggatacaact cctgcaacaa tcgtaaacta tcctctctat ccaccaaaat caaatccgat
    10921 ttttcaaaag ttacatctaa atccctaaag gtatcttgag gattacgtcc aatgaacaga
    10981 ctcaacagtt taggattagt actaggtaaa cgatctagta ccaactggtc atgatgagaa
    11041 tgagaaggaa gcataaaaat atcctgatcc ttcacatacg tttgcagata attctcaaaa
    11101 aattctgcca ccagatctcc catattttga taggtaagca ttttaaagcg ataacccaaa
    11161 attggattga cttctacctg tcctccttct tttaaacgct ctctaaattg ccaaactccc
    11221 ttaggattca aatattcttg ataggctgct tgcccatctt caaaaaaaat aatactcgat
    11281 ataaaccctc gatcatccat gatatggtgc atatgtattt gaccattcgg tccaaaatac
    11341 tgtatatcac tgatgaaacc ttcaactcca tgttcaacct tagcaaattt cttcccattt
    11401 ttttgaacga taatcgtaaa gggactataa ataaattcac agtcatcatc ccactcaata
    11461 tctctaatgc ttaaaacttg ggtatggaga ttatgaaaat cttgcataac atcaaaaacg
    11521 gaataagtat ccgtttctaa cacaccatgt ctatataaaa aataacgtag atgaggctgg
    11581 taagctaata ctaatagacg agaatctata tcttgctctt gaaagagccg aatctggtga
    11641 aaggtatcat caaactctag acgaaaatga gaaaaatacc atggagtgat atctgcatgc
    11701 catgttcttt ctgacccata ccaagctgga ataaaataat acataaaagt ctcctaaatc
    11761 aaaggtctat aggtctcatt tagccttaaa gccttgacct cgtctctaat cgtaaaaatc
    11821 atgcccccga acatcataaa taagcctgga atcattgcca atcgtaataa cttttcatca
    11881 aacaaaacaa aaagcatggg accacctgcc ataatcacat taaagagacc acctatgact
    11941 gagaaacgaa ggaccaatcg attaataaat cgactagtat ccgcacctgg ataaatacca
    12001 taaatgtatt ctccagattt tttcatacgg tctgcaatct cttctccatt catcgtaaca
    12061 aaagcaaaaa tgatactaaa taaaaataag accgaaatat aaacatagac ccacaaaggc
    12121 ttcccaacca taaattcctt tgataaagcc gctagccctg aatgattagg gaaaataaat
    12181 cccaacaaga tgaacaaata agctggtaca ctaagaaaac tcatcacata catataaggc
    12241 atcccacctg caggattcaa catgatttcg agataagaat agcgtttaaa tcgattgtgt
    12301 aagccaattt tattaacagg aaccaaatag cgagctcgat acataagggc aagcaagtaa
    12361 gaaaatataa tggtcataaa aacaagcaag acaacaatcc ctgtagggat ataaaccagt
    12421 ttaaaagttt caataacatc ctgagggata tttaaaacaa tgctggatag gaggattaca
    12481 atagaacccc caattcccat gctagcattt aaatctgaca accaaacaag aaaaaacgtt
    12541 cctgctatga gtaacaaggt attcattaga gcaaccaaaa ttaccgagta ggaggattgt
    12601 actggcaagc tcaagctaac tgccaaggat tgaatcacag caattagcaa ggtcagatac
    12661 attcttcgac gatcttgaat ttctatagac gtagatgtta aacccaaccg tttagaaaaa
    12721 gaaaacatct gccataaaat catggcggac atccaagggg ataatccaac agaaaaaatt
    12781 gataaacttc ttagattccc acctgttagg gcggctgaga aggctagata ggctgttgaa
    12841 cctcctaaaa aatctttagt atttaggtca acaaaaggga gagtaatacg acttcccaat
    12901 acatagataa acagtagaaa taaggtaaat agtccttttt ttactgctat tgacgaataa
    12961 atcttagtca taaaacactc ccctcacctt cattgtttgt gatatggtaa ataacaattc
    13021 ttttatttca acttttacca tttctttgtt ctgcatcaaa aaacaacttt aatctgaaac
    13081 aaatatcttt atattcctca gtatctgtta attgatggta ttctaaagtt tgcttataat
    13141 cttttaaaag attgacaaat cgaatacgca gaacggagac atccaaacca gccaagacac
    13201 aatccgatat tttcttagaa aacacttcaa caatatctct tgcccaggaa agatcaaatt
    13261 ccgttctcat gatgcttcct gaacgtcttc tatacagata gagattatcg tttataaaga
    13321 cgattttaga ggctaaaaga taaaagcgat gcatagttgc ttcatcatca aagcggcgac
    13381 catttgaaaa gtgaacttca ttgaataatt cttttttaat caacttaaat gtcggcaata
    13441 taaaggccga gctattgaat ttccaatctc ctgcttgacg gttcataatt tcttgtatag
    13501 ctaattcttc gacgcaaaaa tctttccctg ttacataaaa ataaaaattc ccgtcacttt
    13561 cgtcataatt atagtaattc cctataacaa catctgcatc cttttcttgg acttgctgat
    13621 aaagagtttc aatcatattt tcttttacga tatcatctga atcaacaatc gtgatgtact
    13681 ctcccttagc tacactaatc cccatatttt tagcttgtgc tgcccccccc cttattttct
    13741 tgatgcaata ctcgcacacg attatcttgc atcataaaag catcacaaat ttccccagaa
    13801 ttatccgtag aaccgtcatc aactaaaata atttctatat tttgataggt ttgcttcaga
    13861 atatgctcga cacactccac taaatatttt cccgtattgt atatcggtac cacaacacta
    13921 attaattcac tcattactct atctctatat tcttcattct atccactaaa tcatctggtc
    13981 tctccacaga aaaaataccg tcatagagtc catcatccat acttttacgt gtgatatcaa
    14041 aagcgaagat tttcttgcca ctttcttgtg cccttgtaac tacattaaaa acctctccac
    14101 ctgtattaat atctaaataa actatagaat cgtccaatag ccaatcaatt ctactatgta
    14161 atacattttg atatactgtt acattcgtat actgtgatag agaggtcagc tcctcagaac
    14221 aatcacacgg tgcagccaaa tgaaaatgcc aatcaggtaa ccgttgtact aaatactcta
    14281 aatgttttat atctgcactc catgtcacaa gcatcacttg ttttttagac tgattgcttc
    14341 tttcaaaata atttaaatcg gaacgttgaa aagcaatctc tgaccaatcc aaatctctat
    14401 aaacccacca taattcacgt agtctagata tactatatgt attccaaggt ttatcatgac
    14461 tagcatagtg aacaattgta ggtggattgt catctagacg ttcacattct tgagcaaggt
    14521 gataaatatc aacacctacc atataattat atgttttatc taaggctagc caattatcct
    14581 caaaataaat atttaaaata ctctgatccc caagatgaac aacttgattt tgctcggccg
    14641 ctaattccaa taaactattg acaatagagt gttctttcca ttttgcaaca tccattaata
    14701 aaactccaga attaaatcca gattttcgtc cttcataggc atagacatca tcaacagcac
    14761 caattgaata tcctttgaga tctatctcaa acaaagtagc tagttcccca gttacaatga
    14821 tatcggaatc cagatacaat accctatcag attcaaccac ttctgtcgca aaaaatctaa
    14881 agtaagaagc ataatttata tgaggtcctg ttttatagct ttcaaaaagt tctttatcaa
    14941 tgtgtacact gcgaattgta gaattcacaa cttccatttt tctccccaat aatttaaacc
    15001 attcaggagc tatatcactg ttgagaatat aaaaatcaac atctctattg tgatacaata
    15061 cagactttat agtcgtctct aaaggaataa gatagacatt atctgccgct aatactattg
    15121 attttctcat actgttctct acctcatgta agatctgatt gctgcaacca tttttttcgg
    15181 aatggagctt ggatagatac cttcatatga aatctcagaa agattttcag agcaagtatt
    15241 gtcgaaagct atcattggtt ttttgtactt aagcacaaac tcatatgcat cttctaattt
    15301 tcgatcatga ttcaaatcca aatacaaatc tgaagctatt actatttgtt ccaataacat
    15361 tggtaaggaa tttggatata cggtaacatt aggaaaacgc gttaaagcta gtagttcatt
    15421 tgccatattt gtataagcaa caatatgaaa atgaacttct ggcaattgct ccgccaaata
    15481 atcgattttc tcgacacacc aagaattcgt taaattaaca cattgcaact taaatatttg
    15541 tgatttacta ggatacttca cactctttga aaaccattca tttaaaataa cagaccaatc
    15601 catcaaagag tattcccacc aaacttctct tagacgtcca acagaaaatt gattccaagg
    15661 cttatcctga gaaatatagt gtaaaattag tggcagtggt tcgagcggaa tatcaaaaat
    15721 gaattgatgt ttaaaggctg ccgccccata atcatatcct atttgaaaat tatattgatc
    15781 ttcaagggaa ctatattgat ctttaaacaa catattcaaa attgactggt ctccttcttc
    15841 cacattctca tgttcttttt ctgttaagtc aatcaatttt tgtcgaatag tttcagatcc
    15901 ccattttttg ttgttaatca agagaacacc agcattgaag ccgactcctg ctccaaagca
    15961 agaacgagct gctgccaaat aattttcacc taagtctaat tcaaacaaat cggtcaaatc
    16021 accagtcaca atcaaatcac tatctagata gagaacttta tcttctgtta caaaatctgg
    16081 aataaaatag cgtgcaaatg tcatatgatt gatatgaggt aatttattag accaattcat
    16141 ttgaaactgt gagccaatta acttgcagtc aatcaagtcg ccccccatct cttgtaaata
    16201 tattcttatt tgactaaacc attcctgagg aatgtcctga tttagcagat aaattttcaa
    16261 atgactattg tgtctacaga gtgacttcat cgccgtttcg atttgtcgaa tataagcgta
    16321 atcacctgca aatactacag atttcttatc ttgtaacatt ttatcgtctc tctatcttaa
    16381 ttttcagtta tatttattac cattcaataa atagtctaac atttctaaat gtcttgtttc
    16441 cacattctcc ttaagttctc gttgaattgc aatatcatga cgatatttca aatagaactt
    16501 catctggtct aaatatttac tgacatcata tccttttagc gctagcacgg caattcgttc
    16561 taacctaaaa tcacaatcat cacgcatcat cttcggtgaa taagaattag aaagtccttc
    16621 tttcccaacc ctataccaat acaaaacttt attaatatag gttactttct caacctgcat
    16681 aaataattta taattggttc ctatatcctc acacacccga ccaaacggaa actgaacttc
    16741 ttgaaataat tcccttttaa atagaattcc ccaagaagtt aaaaagctca tatcatacct
    16801 ctctaataaa ggaagttctt ccattagaag ttttgaatta taatttttca cataataatc
    16861 ggagtcaaat acgtgaatat agaataagcc atctgactca ttataggttg aataagttga
    16921 aacaacaatt tcagaatgct gctccttcaa agtttctata agaattgata aatactcttc
    16981 agtaacccaa tcatctgagt ctacaaaagt aatataatct cctttagatc tctcgattcc
    17041 aaaattacgt gcatccgcta ccccagcatt ttctttttcg aaatagtgaa aacgactatc
    17101 acgatcagca tactctcgac atattgctcc tgaattatct gtagagccat catttactaa
    17161 taaaacttca aaattttgat aaatctgttt cgataaactg tctaagcagc tagaaataaa
    17221 tggttctacg ttataaactg gtactataac acttattaaa tctttcatac ttctctcttt
    17281 gataaatcaa ctaaaatctg actgttatag gtttcatcct caatgaactg aatatcttga
    17341 tcaaaattat actctgctgt atgtttatgt gaaaatttaa ccttagtttt agcacccatc
    17401 ttcatccact catactcgga gtcaatatgc ccaatatcta agacttggta accctttcaa
    17461 cttaaatgat aggctaaaac ttttgctgta ggacccagca tacataaaat cagtcttcct
    17521 tcagcatgtt tcataatttc ctcttgaatg ttatcgacaa cggaataagc attatgagat
    17581 ggacaaataa tccgtttcac agatttgacc ttatcaaata aatcatttcc aaccccagag
    17641 cgagatgtaa tcccctctac aatcagaata tccctattct cccaaatact ctttagcttt
    17701 tcaaattgtc cttttgcctt actcttatct tcataatcaa tgtatggacg cgagataaag
    17761 gtagagccat accatgaacc cttacacaac tctttataaa aatccatata aacattcata
    17821 tgttctttcc agaatggtgt agcccaccaa gtaaagacaa aacgatcgtc aaaagcatca
    17881 ggtaaacaaa ccactaattt ctcgtcactc tcaagaccca caatatgttt taattggtta
    17941 gctaactcta gatcatgatt ttgataaggt ataggcaaac cccacatcaa attgacttct
    18001 ccgtctccaa aacgaactaa agaagagttg tgttcaataa tataatccaa ggtttcatct
    18061 atccctttca cctggattat atcaagcggt ttttctagca tatccttagt tatcaatttt
    18121 tgcaaatcag ccaacattcg ttcaggatgt tcacttgaat acaacctttg gccattattc
    18181 ccattttgag atttataaaa ggctagtata ggtttagata gatgagccat ttctgcaacg
    18241 atatcggatg ttgaagtcag taggttgata tctaaataag catccatctt ctccttcaac
    18301 tcgtctagct tatctctaga aattgccgga tagagctgaa tattaggata acgattagac
    18361 aaatcatata attttggccc catagctgtc caagcagcaa cttgaaaaac aacatttggc
    18421 aactgtccag ccaagtaatc caattgttcg atatcctgag aagccgtaaa cgttaaacaa
    18481 gttaaacttg gaagttgtcg aaaacaggta agatgtccgt ttttcttaaa atctttaatc
    18541 gtatcaatca taaaggaaac tttcccattt tcgaaaacga tttgtcctaa ctctccgtgc
    18601 tgagtcgatt gaaaagctag tagcgttttt tcttgttctt gcaaagattt taaaaagttc
    18661 tcatctgcac taccatgatt gatatccaaa tatagatttg ttgacttttt cagcttatct
    18721 aatacaggag gaacaatttg tggatgcaat ctcacattat tatatacagc taattttttt
    18781 aatttatctc ccatatccgt ccaagctgcg atatgaaata ccacctcagg tagagctgta
    18841 actagctctt ctattccttc taaatcttgg gaattcgtta aggttaagca agaaaattcc
    18901 ttatctagag gcgatattag ttcaaattct cccaaatgat gttgtaaaat ctgattccac
    18961 tccaaatcat ggaattccca ccataaatca cgataacgat tggctgtcaa ggtggtccag
    19021 ggttttctgt aggtcgtaaa atgaatcacc actggtttat cattaaaagc cagatgttcc
    19081 tgccaattgt tatacaaagc cacaatatca tgccctactt gtaaattata agctcgacct
    19141 agttctaacc aatcatcttg taagacctga ttaaaaatcg tttgatcacc attaaaatgc
    19201 tcgaaacggc cttcttcaac ttccttcatt gtaacaatgc tctgttcaat tagtcgttct
    19261 tttaatttct cttgacgcca tttttcattg ttgattagta gaacaccagt attaaaacct
    19321 tgaccatttg catctctaat cgctgcgata taatgttctc ttacatcctg ctcaaataga
    19381 ctatctaatt tatcattgat aagcaagtca caatctaaat acaaaaccgt atcttcttgt
    19441 atataatctg ggataaaata cctagcatag gcaattgaac taatgtgagc ctgttttttc
    19501 caatcttgaa acacagtttg ttcaggtagt ttaacatcga taatctcact acctaacatg
    19561 ctagctattt ttcgtggctt tcgaaaccaa tctggcatga tatcttgatt taaaatataa
    19621 atcttaacat ctcgattgtg gtataaaata gattttatgg ttgtttctaa ctgcctggtg
    19681 taattacgat taccagctaa tacaattgtt ttaatcatct tgttctctta ttcaactccc
    19741 aaatggtaaa ttgcatctac caataatttt ttagtaaaat aaccttcttt tagcaaataa
    19801 ctaaaggttt ttatccgatt cgtcatttct tgatattctt gtagattcat tttatcaact
    19861 atctcatgaa cttcctctag actattcgcc acaaagccca agccttgatc tactataaat
    19921 ttagcagttg ataagttgct cggtacaatc actgggatac cagctgttag ataggtacta
    19981 accttatgag atatattcaa ggtatagtat tggttacttt ctccctcatt ttgatgggtt
    20041 ccccagacaa ggccaaatcc accctttgat aattctagca acaattcctc atcttttttc
    20101 catccttcga tgctgagatt tctagcacta gaaccggcct cccctttatt tgaaaatact
    20161 ctcaaaggcg tatcttgaga ccaattttgt aagtctggaa aacgctctaa acttccagca
    20221 aaaaaagttc tttttttaaa agcaggggtg tataaggata aatcatgagg atgatcccac
    20281 atcccttgaa caagaatctt cttagtcgtc aatccttctt ccatcaggcg tgttttcatt
    20341 ctctctgacg gcactatcaa aacatctgat agattataca tgtacatata gtctttcatg
    20401 agataatagt tactatcaaa catgagggga acaacatcat ggataaagca aataattttc
    20461 acctgcatat cctttagctt atcaaacaag agacgatcaa attcaaagcc attccaggtt
    20521 ggagactgaa agactaagat atcaccaata gagatactgg ccataatacc atccagacgc
    20581 ttattcattt cagaaggact atctgaagca atgttgtaaa aataaatacc aacctctcta
    20641 aatcccagtt gactagctat cttttgaaca gcattttgag ctaagataac cgtactatca
    20701 ccagccatgc cgtataaatt tgttaaatgt aatttcatat tacgctatct cattttttat
    20761 tactatctac ggaagtaaag acaatttttc catcattctt ctataaatct ctgtgaactg
    20821 catattttgt tcttctaatt ttgctactac tctttgtaat ctatttttat aaatcaaaat
    20881 atgattggtc aagtcatagt ctgaacttgc taaaatagct atcttttctt ccctagcccc
    20941 aatttcatca tacatacgct tttcactcca tgtattcgat aaagtatcag ataaaccaac
    21001 acggtaccag taaacacaac gattcaaata gactatcctc gaagctctta gaagtacctt
    21061 ccatgtccag taagtatcct ctgcaatttt tcctatagga aatggtaaat cttgtagtaa
    21121 ctctctcttg aataacttca agacagctac agtccagttc ccatttctga cttcttcgac
    21181 accttcccta tccataattg ctttaccttc tatcacttct agagaatcat ctggatccgt
    21241 aacataagtc atatacacat agcgtgtttc atcataagaa ttataacgcc cgatactaat
    21301 atctgcgttt tcctttttca aagcaccata taatcggtct aaagcatcat gttccaacca
    21361 atcatcagag tctacaaaag taatgtacgc cccccccccg aacattcaat acctaggtta
    21421 cgagctgatg aaagaccgcc gtttgctttc tcaaaatatt tgaaacgaga atctttctct
    21481 acaaattctt cacatatttt ggatgaatga tctggagagc catcattgat taataaacac
    21541 tcaaaatttt gatacgtctg attctgaatg ctatccaaac acattctcag ataattttcc
    21601 acattatata ttggaacaac aatactgatt aactcattct ccattgactt attctcctaa
    21661 aaatttgcga atagcatcta ccatcttatc tggctcagtt gctgaaaaaa tccgacttct
    21721 tcctttgcta tcatgactag tattatcaaa actaaagatt ggcttattgc gcctacagac
    21781 ttcttctata atgttgacaa tctctccttc atggttaata tcaagataga aatccatctt
    21841 atccaaggtt tctttcatat taaaagaatt aaaatttgga aaaagtgaaa gattcagata
    21901 tctctgtaaa tcaacaacag agggagcaaa atttgtatgc gccaagatcg taaagtgcac
    21961 ttctggcaat tccgatagga gatattccaa ggactccatt gcggcactat ttgtaaaaat
    22021 acaagtatga tataagggaa cacccactaa ctccttaaaa gagcgtttaa cgatatctga
    22081 acttaacaac acatcgttcc agttcaatcc ataataaaac caccagagtt tacgaaaacg
    22141 tgtaagtgtt aaatgagtcc acggtttgaa ctctgtagta taatggacaa tcttaggctc
    22201 gtaatttcca taatagttgg attggtacca gtcagcatta ccttttaaat gtaaaaactg
    22261 atctaaacca accatgaaat tatactcttt gtccaaatgt agccactggt caccgaaata
    22321 tatattgaga acaccttggt ccccatagac atgttcatgg tgttgagcag tcaactctag
    22381 caaattttgt gttgattttt tttctcgcca tttttttgca tcaatcaata acatacctga
    22441 attaaatgtt gttaaccaac catcttgatc aaaatcttga acagctgcga tagcgtatcc
    22501 ttgcaaatcc aaaaaaaata attcatccaa attggcattc acaatgatat ccgagtctaa
    22561 gtagagtacc cgatcttcaa gcacttcgtc tgctatgaaa tacctaaaga aagttgcata
    22621 ggacaggatt gcatttggca agtggaaatc acgaagattg tgatctgctt ttttgacatt
    22681 aataatctca gaaccaataa ccttcagacg atttctcatc aaaagaaacc attcactagg
    22741 cagatcatca ttaaacacat aaaatttcac ttcgtgatta tgaactgaaa ctgactttat
    22801 aacggtttct accttatcaa gataatgaat atcagctcct aaagcaataa cttttctata
    22861 cattcttatc tcccgatttt tctattttat aaacaaatca tctattttac caactgaaaa
    22921 aacgtgattt ctacttttga gatattgatt ttttacttct gtttcatcaa caattacagt
    22981 atccttttct tcgatgatat cttttcattt ctaaatctaa atgttgaatc aaaaaatctt
    23041 caaatcaatt atgctatgaa taagatttct ttcgtgttct gcaaaattta caactatcta
    23101 cttatagatt cggcattatc aaatagatta aagactcacc attaagatat cctctgaagt
    23161 catgtttggt tttcttccaa ccttagaaag tttcatctaa cttgatttta tattactcat
    23221 taaactctca tctttacaaa aagatttcca ttattataaa ctacactgtt tgatggaata
    23281 tcattgcgta tgagacaacc tgctccaata atggtattat ctccaatcgt cactcctttt
    23341 aaaatcgtga cattacttcc tatccagcag tctctgccaa ctctaatcgg cgctgtagtc
    23401 cactgccact tttcaatctt ttcagcggta taaatatggt cgtgatcata aaaacgaaca
    23461 ccctctccca tcatggtacc agctccgatt tcaattcttt ccatacagtt aaaactacaa
    23521 gagttgttga taaaaacacc attattcagg attaacttac cagaagagac atgaaaattt
    23581 tcaaaatttc tacaggcgac attggctcct acaatgaagt gagccttatc atcgatttta
    23641 aagacattcc aagtcccatc ttgaatgatt ttctcttgat gattggtgcc tactctgact
    23701 tcattcagat agtctctcac tctagaaacc aatttgtctg gtgtacttga tagaaaaact
    23761 tcctgcccct gatttccatg gactgtattg tcaaatgcaa agacagattt tccagcctgt
    23821 acaaacttgg agacaatagc atctacttct tgaaagtgat tgatatctag taaaaagtca
    23881 caggtatcga ctaaaaagtt tagctgttct tcatttgtaa tcgttcggag acggacatta
    23941 tagttcgttt ctaagctacg gattttctcg gaaaattgaa caggtgctgc aatatgaaaa
    24001 cagatatttg acaaggattc aaggagataa tccaactctt caatctgatc actaaccgtc
    24061 aaaatcagag catctcctac taaatgattt ttcttcattt gcttatttct ctcaatttct
    24121 caatcattgc ttgaacttgg tcaacagcat atgcctcctg acctacttca taggttttag
    24181 tattttcaaa ggataagata ggcttgccaa gattagcaaa ttgatcgaga atttcttctg
    24241 tcttttcgcc atgattaata tctaaaagta cttgactggt ttctaccaat tcattatcga
    24301 catctaccaa atagtgaatt ccgttaaata tagtcacgtt tggataaatt gtcatctgag
    24361 ccaatcgatc actaactatt actctagctg ctatcttaaa ctgaatatca ggcaaggatt
    24421 gaaccaatgt ctcaatttgt tcaatatggt ctgaggcagt atagattaga caagtgaaag
    24481 gttcctttat tggatagatg tgagatcttt gtaatggatg taaatgatgg ttttgtccca
    24541 attctgtcca ttcaagccca tgatagtacc accaaacttc acgataagtt tgggccgcca
    24601 aatctttcca cggttttcta tgggaaaggt agtgaataat agcaggataa tcctgaccct
    24661 caggcaattg ataatcagca aactgtttat gaatgacaat atgattataa tcaaaatcca
    24721 attccagcca tttatgttca aaaagcatat tcaagatgct ctgatctgcc tgatccacct
    24781 tatcatgcca ttcattggtt aaatcaatta atttttgggt catattctct tttttccaaa
    24841 aagcattgtt taccaagaga acaccggcat taaagatttc ttgaccaaaa taagctctgc
    24901 ccccaaaatc tctaacagca gccaaaggat aatcttgtaa gtctgtagca aacaagtcat
    24961 ccagattttt cgttacaact agatcacagt ccaagtagag ggccttgtct tcttgcacga
    25021 aatcagctat gaaatagcgt aaaaagactg tgtaactaat atccgattta taacatgaaa
    25081 tttgctcaga agttacccga caattaataa tttctgagtc aaacttctct aatcgcttat
    25141 ttaattgttt gatccattca tttggaaaat cgctatgaat caaataaaaa cgaagcgagc
    25201 gattgtggta acagatagac ctgattgtcg ttaaaacttg gtctacatag gcataattcg
    25261 ccgctaggac aatggctttc ttttcacttg attcttgtac cgttaactga tttaaaagtc
    25321 tttttttcat ttcaaactct ttatacgttg ctgtgtcaga taaaccacta gcttgaccgt
    25381 tggcgagact gaattccaac atttgacgat aaatcgccaa atgcttttct aaagggtaac
    25441 ccatattagc tagtagcgta atgcgttcgg acatggcatc aaccaatgca tgcatccact
    25501 tttctgtcca aactcttgat aaactacctt ttctaatccg attattcttc atcatcacgt
    25561 ttacggcgtt tcgcaaacaa acccaagcca gtcacagctg ccaaagctcc aaggagtaca
    25621 gatgacttgg aagcttctgt acctgtattt ggcaattgct gtcttgactt acctgctgaa
    25681 gttgacgcgc ttgcgcttgt tgatgccgat tcagacgctg aagccgacgc actcgttgat
    25741 gctgaagctg aagcgcttgt cgatgctgac tcagacgcac tcgttgacgc tgaagccgac
    25801 gcactcgttg acgctgaagc tgacgcactg gttgacgctg aggctgaggc agacgttgat
    25861 gcggattcag acgcacttgt acttgctgaa gccgaagcgc ttgtcgatgc tgaggctgaa
    25921 gcactggttg aggccgattc agaggcactt gtacttgctg aagccgacgc actcgttgac
    25981 gctgaagctg aggcgcttgt gcttgctgaa gctgaagcac tcgttgacgc tgaagctgac
    26041 gcactcgttg atgctgaagc tgaggcactt gtcgatgctg aggctgaggc actcgttgac
    26101 gctgaagctg aggcgctggt acttgctgaa gctgatgcac tggttgacgc tgaagctgaa
    26161 gcactcgttg atgctgagac tgatgcactg gttgacgctg aagctga
//
LOCUS       DAVLWA010000020        26126 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLWA010000020 DAVLWA010000000
VERSION     DAVLWA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26126
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.020"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            374..1537
                     /locus_tag="VYN80_000829"
     CDS             374..1537
                     /locus_tag="VYN80_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1540768.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVTFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSQELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1534..2211
                     /locus_tag="VYN80_000830"
     CDS             1534..2211
                     /locus_tag="VYN80_000830"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1540769.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEASAPGQISLASMKKI
                     REILDEA"
     gene            2201..3055
                     /locus_tag="VYN80_000831"
     CDS             2201..3055
                     /locus_tag="VYN80_000831"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1540770.1"
                     /translation="MKLDGYTRLTAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3074..4141
                     /locus_tag="VYN80_000832"
     CDS             3074..4141
                     /locus_tag="VYN80_000832"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1540771.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKFYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4151..5317
                     /gene="aroC"
                     /locus_tag="VYN80_000833"
     CDS             4151..5317
                     /gene="aroC"
                     /locus_tag="VYN80_000833"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1540772.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHKPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5327..6430
                     /locus_tag="VYN80_000834"
     CDS             5327..6430
                     /locus_tag="VYN80_000834"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1540773.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEYLITENTDYTVVIK"
     gene            6441..6779
                     /locus_tag="VYN80_000835"
     CDS             6441..6779
                     /locus_tag="VYN80_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1540774.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6871..8154
                     /gene="aroA"
                     /locus_tag="VYN80_000836"
     CDS             6871..8154
                     /gene="aroA"
                     /locus_tag="VYN80_000836"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1540775.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8147..8623
                     /locus_tag="VYN80_000837"
     CDS             8147..8623
                     /locus_tag="VYN80_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1540776.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGESAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8620..9468
                     /gene="pheA"
                     /locus_tag="VYN80_000838"
     CDS             8620..9468
                     /gene="pheA"
                     /locus_tag="VYN80_000838"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1540777.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSIHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9465..10739
                     /gene="psr"
                     /locus_tag="VYN80_000839"
     CDS             9465..10739
                     /gene="psr"
                     /locus_tag="VYN80_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1540778.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11018..11863
                     /locus_tag="VYN80_000840"
     CDS             11018..11863
                     /locus_tag="VYN80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1540779.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11853..12956
                     /locus_tag="VYN80_000841"
     CDS             11853..12956
                     /locus_tag="VYN80_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1540780.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTNVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12958..13944
                     /locus_tag="VYN80_000842"
     CDS             12958..13944
                     /locus_tag="VYN80_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1540781.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13960..15393
                     /locus_tag="VYN80_000843"
     CDS             13960..15393
                     /locus_tag="VYN80_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1540782.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15422..16456
                     /locus_tag="VYN80_000844"
     CDS             15422..16456
                     /locus_tag="VYN80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1540783.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16656..17393
                     /gene="mecA"
                     /locus_tag="VYN80_000845"
     CDS             16656..17393
                     /gene="mecA"
                     /locus_tag="VYN80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1540784.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17544..18830
                     /locus_tag="VYN80_000846"
     CDS             17544..18830
                     /locus_tag="VYN80_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1540785.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDHDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFTESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18832..19701
                     /locus_tag="VYN80_000847"
     CDS             18832..19701
                     /locus_tag="VYN80_000847"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1540786.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19777..19854
                     /locus_tag="VYN80_000848"
                     /pseudo
     CDS             19777..19854
                     /locus_tag="VYN80_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            19996..20934
                     /gene="msrB"
                     /locus_tag="VYN80_000849"
     CDS             19996..20934
                     /gene="msrB"
                     /locus_tag="VYN80_000849"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1540787.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20992..22716
                     /locus_tag="VYN80_000850"
     CDS             20992..22716
                     /locus_tag="VYN80_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540788.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22718..24466
                     /locus_tag="VYN80_000851"
     CDS             22718..24466
                     /locus_tag="VYN80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540789.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGRVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24858..26117
                     /locus_tag="VYN80_000852"
     CDS             24858..26117
                     /locus_tag="VYN80_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1540790.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
BASE COUNT         7697 a         4901 c         5912 g         7616 t
ORIGIN      
        1 tagtatattt agtttgtaac aaaaggcttt tctataaaat caaattttcc agatatctac
       61 tgaagctgat ttattttttc atctttcttc tactaccttt gtcactaaat ttgacagaca
      121 catattgctg atacaacctc tctacctata atctcacagt taacctgagg ctagtttcat
      181 agtttgctat ttgattttcg ttgataaaaa gaaatgctcc tccaagacgg aagagcattt
      241 tttattgtga ttcttacaca tttacttcct agttttgcag gaagtctagt ataccatttt
      301 ttaaaagaag agtaaaaaag caagggtagt ttcttttttg agaattgtct aaaagtttga
      361 tataatggta gttatgaata gaataagagt cagcaaaagg gttgaaaaga agcttgctaa
      421 ggggctagtt ttactagaag ccagtgatct tgagaatgtc aatcttaagg atcaggaagt
      481 agaggtgcag ggtcaggaag gaaactttct tgggactgcc tacctttctc agcaaaacaa
      541 gggcttgggc tggtttgtca gcaaagacaa ggtgaccttc aatcaaactt tctttgaaac
      601 gttgtttaga aaagccaaag aaaagagaaa cgcctactat caagatgatt tgacaactgc
      661 ctttcgtctc tttaatcaag agggagatgg ctttgggggt ctgacagtgg acctttatgg
      721 cgactacgcc gtcttttctt ggtataactc ttatgtttat cagattcgtc agactatatc
      781 agaagccttt agacaggttt tccctgaggt tttaggggct tatgagaaaa tccgctttaa
      841 gggttttgac tatgaatctg cccatgttta tggtcaagaa gcacctgact ttttcactgt
      901 tttagaaaat ggtgtcctgt atcaagtctt tatgaatgat ggcttgatga caggaatttt
      961 cctagaccag catgaggttc gcggtagttt agttgacggc ttggctatgg gtaaatcctt
     1021 gctcaatatg ttttcctaca cagcggcttt ttcagtagct gcggccatgg gaggagctag
     1081 ccagacaact tctgttgatc tagccaaacg ttcacaagaa ttgtctcaag cgcattttca
     1141 ggcaaatggg ctcagcacag acgagcatcg ttttatagtc atggatgtct ttgagtattt
     1201 caaatatgcc aaacgcaaag acttgaccta cgatgtgatt gtcctagatc cgcctagctt
     1261 tgctcggaat aaaaaacaaa ctttctctgt ggccaaggat tatcacaagt tgatttccca
     1321 gagtcttgag attttaaatc cgggagggat tatcattgcc agtaccaatg ctgccaatgt
     1381 ttcccgccag aaatttacag aacaaattga taaaggcttt gcaggaagaa gttaccagat
     1441 tttaaacaaa tatggtcttc cagcagattt tgcctataat aaaaaagatg aaagtagtaa
     1501 ttacctcaag gtgattagta tgaaggttag taaatgaaat taatcgtttc agtaatgcca
     1561 agaagtttag aggaggctca ggctctggat gccacgaggt acctggatgc cgacatcatt
     1621 gaatggcgtg ccgactatct gcctaaagaa gcgattttgc aggtagctcc agccattttt
     1681 gaaaaattcg caggccgtga gttggttttc acgctacgaa ctcgctccga aggtggagaa
     1741 atcgaccttt ctccagaaga gtatatccat ctaatcaagg aagttgcgca actctatcaa
     1801 ccagactata ttgattttga gtactatagc tacaaggatg tttttgagga aatgctggac
     1861 ttcccaaatc ttgttttgag ttaccataat ttccaagaaa cacctgagaa catgatggaa
     1921 atcttgtcag agttgacgat cctaaatcca aaacttgtta aggttgcggt gatggctcac
     1981 acggagcagg atgtcttaga cttgatgaac tatacacgag gctttaaaac cctcaatcct
     2041 gaacaggaat atgtgaccat ttctatgggt aaggtgggca aggtctctcg tatcactgcg
     2101 gatgtgactg gttcgagttg gtcttttgcc agtctggatg aggctagtgc ccctgggcag
     2161 atttctctag ctagcatgaa aaaaatcagg gagattttgg atgaagcttg atggctatac
     2221 acgtttaact gcagttgttg ccaatcctat taagcattct atttctccct tcatccacaa
     2281 tagagccttt gaggcgacag ctaccaacgg tgcttatgtg gcttgggaga ttgaagcgag
     2341 tgacttggca gaaacagtgg ccaatattcg tcgctaccag atgtttggca tcaatctgtc
     2401 catgccctat aaggagcagg tgattcctta tttggatgag ctgagcgatg aagcgcgctt
     2461 gattggtgcg gttaatacgg ttgtcaatga gaatggcaat ttaattggat ataatacaga
     2521 tggcaaggga ttttttaagt gcttgccttc ttttacaatt tcaggtaaaa agatgaccct
     2581 gctgggtgca ggtggtgcgg ctaaatcaat cttggcacag gctattttgg atggcgtcag
     2641 tcagatttcg gtctttgttc gttccgtttc tatggaaaaa acaagacctt acctagacaa
     2701 gttacaggag cagacaggct ttaaagtgga tttgtgtgct ttagaatatg tttctgaact
     2761 gcaagcaagg attgccgagt cggatttgct agttaatgcc accagtgtgg gcatggatgg
     2821 ccaatcctct cctgttcctg aaaacatagt cttaccagaa actctcttag ttgcagatat
     2881 catataccaa ccctttgaaa caccgttttt gaaatgggct agaagtcagg gcaatccagc
     2941 agtcaatggt ctgggaatgt tactctatca agctgcagaa gcttttcaac tgtggacagg
     3001 caaggaaatg ccgacagaag agatttggca gtctttaaca gaaaaatacc aataaagaaa
     3061 gggagactct cctatgaaaa tcagaatcga tattcctcat catccttatg atattcagat
     3121 tgaaaaaggt tgtatggccc aggctggtca gtggttgcga gaactctggc aaccgcaaaa
     3181 ggtagtcatt gtgacagata accatgtagc ctctctctat gcagagaagg tcaagctcag
     3241 cctagaagat gctggttttc aggtagctgt ttttgatttc ttagaaggtg aagaaagaaa
     3301 gaatttaact actgttcaga aagtctatga atttctagtc aagcaaggtc tgactcgtag
     3361 cgatggaatc gttgctcttg gtggtggcgt tgttggggac ctggctggat ttgtagcctc
     3421 tacctatatg cggggtattc actttgttca gattccgact agtttgacag ctcaggttga
     3481 ttcttctatc ggtggaaaga caggcgttaa cacaccattt gctaaaaata tggtggggac
     3541 ctttgcccaa ccagatgggg ttctgattga tccacttgtt cttgaaaccc tcggaaaaag
     3601 agagttgatt gaagggatgg gtgaagttat caagtatggc ttgattgagg atccagaact
     3661 gtgggctctc ttgacgggac tgaatggttc tgttgagagc attttggaac atgcagagac
     3721 cttgattgaa cattcttgtc aggtgaagcg caagatggtg gttgaagatg agttggacaa
     3781 tggtattcgt ctttacctca attttggcca cactattggc catgccatcg aagcgactgc
     3841 cggttatggc aaggtcatgc atggagaggc tgttgccatg ggaatggtac agatttccaa
     3901 ggttgctgag gaaaaaggcc tcatgccagc tggcattacc caatctatca cagagatgtg
     3961 tcagaaattc ggcttgcctg ttgactatga aaattgggaa gttgacaagt tttatcaggc
     4021 tcttactcat gacaagaaag cgcgtggtaa caccttgaaa ttggtcttgg tgccagagct
     4081 tggttcggcg accattcacc cagtttctct ggaagagatg aaagactact tggtaaaata
     4141 aggagagtgt atgagatatt taactgcagg agaatcacac ggcccccgtc taacagctat
     4201 tattgaggga attccagctg gacttccatt gacagctgag gatatcaatg aggaccttag
     4261 acgccgtcag ggtggctacg gtcgtggtgg tcgtatgaag attgagagtg accaggttgt
     4321 ctttacttcg ggcgttcgcc acgggaagac gacaggggcg cctattacta tggatgtcat
     4381 caataaggac caccagaaat ggctggacat catgtctgcg gaggacattg aagaccgcct
     4441 taaaagcaag cggaaaatta ctcatcctcg cccaggtcat gccgatttgg ttggggggat
     4501 taagtaccgt tttgatgatt tgcgaaattc tttggagcgt tcatcagctc gtgaaaccac
     4561 catgcgggtg gcagttggtg cagtagccaa acgcctcttg gctgagctgg atatggagat
     4621 tgccaaccat gtcgtggtct ttggtggcaa ggaaatcgat gttcctgaaa atctgacagt
     4681 tgctgaaatt aagcaaagag ctgcccagtc tgaagtttct attgtcaacc aagaacgaga
     4741 acaggaaatc aaggactata ttgaccaaat caaacgtgat ggtgatacca tcggtggggt
     4801 tgtggagaca gtcgtcggag gcgttccagt tggtcttggt tcctatgtcc aatgggatag
     4861 aaaattggat gcaagattgg ctcaagctgt tgtctctatc aatgccttta aaggggtgga
     4921 atttggtctt ggctttgagg ctggttatcg taaaggcagc caagttatgg atgaaattct
     4981 ctggtctaaa gaagacggtt atactcgccg taccaataat ctaggtggtt ttgaaggtgg
     5041 tatgactaat gggcaaccca tcgttgttcg tggggtcatg aaacccattc ctactcttta
     5101 taaacctctt atgagtgtgg atatcgaaac ccacaaacct tacaaggcaa ccgtggagag
     5161 aagtgatccg actgctcttc cagctgcagg aatggtcatg gaagcagttg tagcaacggt
     5221 tctggcgcaa gaaatcctcg aaaaattctc atcagataat cttgaggaac taaaagaagc
     5281 ggtagccaaa caccgagact atacaaagaa ctattaagga gttcctatgg caaaaacaat
     5341 ctatatcgca ggtcttgggt tgattggagc ctctatggca cttggtatca aacgcgatca
     5401 tccagattat gaaattttag gttataatcg tagtcaagct tcgagagata tcgccttgaa
     5461 agaaggcatg attgaccgtg caacggatga ttttgctagt tttgctcctt tggcagatgt
     5521 cattatcctc agcttgccaa tcaaacaaac tattgctttc attaaggagt tggccaattt
     5581 ggatttgcga gaaggcgtta ttatttcaga tgctggttcg accaagtcaa ccattgtgga
     5641 tgcggcggag cagtatttgg ctggcaagtc tgttcgcttt gtcggggccc atcccatggc
     5701 tggtagtcac aagacagggg ctgcttcggc agatgtcaat ctttttgaaa atgcctatta
     5761 tatctttaca ccttcaagcc tgacaagtca ggacacgctt aaggaaatga aggatctgct
     5821 ttcaggtctt catgctcgtt ttatcgagat tgatgccaag gagcatgatc gtgtcacttc
     5881 tcagattagc cattttcctc atattttggc ttctagtctc atggagcaga ctgcggtcta
     5941 tgctcaagag catgagatgg caaggcgctt tgcggcaggt ggttttcgag atatgacccg
     6001 aattgcggaa agcgagccag gaatgtggac ctccattctc ttgtccaata gcgagaccat
     6061 tctggataga attgaggatt tcaaggaacg tttggaagcg attggtcagg ccattagtaa
     6121 gggagatgaa gagcaaattt ggaacttttt taaccaagcg cgtgaacaac gccagaccat
     6181 ggaaatccat aagcgtggtg gtgtggatag ctcttacgac ctctatgttg acgttcctga
     6241 tgaagaagat gtcatcctgc ggattttgga actgctacgt ggaacttcct tggttaacat
     6301 ccacatcaac gaggaaaatc gtgaggatat tcacggaatc ctacaaattt catttaaaaa
     6361 tgctcaagac ttggaaagag ctgagtatct cataacagaa aataccgact atacagtcgt
     6421 catcaaataa ggagaagaac atgtcaaata tttacgatag tgcaaacgaa ctcagtcgcg
     6481 gtctacgcgg attaccagaa tacaaggctg ttaaagcagc taaagatgcg attgcagcag
     6541 atgctgaggc aagcaaaatc tttacagaat atcttgcctt tcaagaggaa attcaaaaac
     6601 tagcacagac aggtcaaatg ccagatgcgt ccttccaagc taagatggaa ggctttggta
     6661 aacagattca aggaaatagc ctcttgtcag aattctttac caagcaacaa caattggcca
     6721 tttacctttc tgacattgaa aaaattgttt tcgaaccagt ttcagaattg ctgaaataag
     6781 aaaagaacga tcctaaagtc aggaattttt aaggaatttc tggcttttta tgataaaata
     6841 ggtaaaagta ttgcaagtat gaggtccagt atgaaactaa aaacaaacat tcgccattta
     6901 catggtagta tccgcgtccc aggtgacaag tctatcagcc accgttccat tatctttgga
     6961 agtttggctg agggtgagac caaggtttat gatattctgc gaggtgaaga cgttctttcg
     7021 accatgcagg tttttcgtga ccttggtgtt gaaattgagg ataaagatgg ggttattacc
     7081 attcaaggtg taggcatggc tggcttaaaa gcgccgcaaa atgcccttaa tatgggaaat
     7141 tctggcacct cgattcgcct gatttcaggt gtccttgctg gtgcagattt cgaagtagag
     7201 atgtttggag atgatagtct ttccaaacgt cctatggacc gtgtgaccct tccactgaaa
     7261 aaaatgggcg tcagcatctc agggcaaact gaacgagact tgcctcccct tcgcttaaaa
     7321 gggacgaaaa acctaagacc tattcattat gagttgccaa ttgcctctgc ccaagtcaag
     7381 tcagccttga tgtttgcagc cttacaagct aagggggagt cagttattat cgaaaaagag
     7441 tacacccgta atcatactga agatatgttg aaacaatttg gtggtcattt aagtgtggat
     7501 ggtaagaaaa tcacagtcca agggccacaa aaattgacag gacagaaggt ggtcgtacca
     7561 ggagatattt ccagtgcagc cttttggtta gtcgcaggtt tgattgctcc aaattctcgt
     7621 ctagtgctgc agaatgtggg gatcaacgaa actcgcaccg gtattattga tgtcattcgt
     7681 gccatgggtg gaaaattgga aataactgaa atcgatccag tcgctaaatc tgcaaccttg
     7741 attgttgagt cttctgactt gaaaggaaca gagatttgtg gcgctttgat tccacgtttg
     7801 attgatgaat tgcctattat tgccctactt gcgacccaag cccaaggtgt aacagttatc
     7861 aaggatgctg aggagctcaa ggtcaaggaa acagaccgta ttcaggttgt ggcagacgcc
     7921 ttaaatagta tgggagcaga tattactcct acggcagatg ggatgattat caaaggaaaa
     7981 tcagctcttc acggtgctag agtcaatacg tttggtgacc accgtatcgg catgatgaca
     8041 gctatcgcag ccctattggt tgcagatgga gaggtggagc ttgaccgtgc agaagccatc
     8101 aataccagct atcctagttt ctttgatgat ttggagagct tgattcatgg ctaaggtgtt
     8161 attagggttt atgggggctg gaaaatcgac tattgcaaga ggcttggacc ctaattacct
     8221 tgatatggat gctctgattg agaagcgcct aggtatgtcc attgcgaatt ttttcgctga
     8281 aaagggagaa tcagcctttc gtcaggtaga atcagaagtc ctagctgatt tactacaaac
     8341 agaccaagtc gtgtcaactg gaggaggagt ggttatttct cagagaaatc gtgatttact
     8401 caagactaat acagataaca tctacctgaa agcagatttt gaaaccctct accaacgtat
     8461 cgcagctgat aaggacaatc agcgaccgct ttttctaaat aatagcaagg aagaactagt
     8521 agctattttt caagaaagac aggcttggta tgaggaagtg gctagtcggg ttttggatgt
     8581 gaccaagcta agcccagagg aaattataga ggaactaaga tgaaaattgc ttatctaggt
     8641 cccaagggat cattttcaca ccacgttgtg cagacagctt ttcctcatga ggaattgcag
     8701 gcttttgcca acattacaga tgtcatcaag gcttatgagc aaggattagt ggactattct
     8761 gtggtgccag ttgaaaattc tattgagggt agtattcatg aaaccttgga ctatcttttt
     8821 catcaggctc acatccaagc agtagcagaa atcgttcagc ctattcatca gcagttgatg
     8881 gtggttccag gccatactaa gattgaaaag attttttcac atccacaggc cttggctcaa
     8941 ggaaagaaat tcatcgatga acaatatcca gaggctcaaa tcgaggtaac agctagtaca
     9001 gcttatgcgg cccgttttat ttccgaacat ccagatcagc cctttgcagc agttgcacct
     9061 agaagttctg ctgaagaata tggattggaa ctgattgccg aggatattca ggaaatggaa
     9121 gccaatttca cacgtttctg ggttctagga gctgaaaagc ctagtattcc cttgcaagca
     9181 caaactgaaa agatgagttt ggccttgaca ttacctgaca accttccagg tgcactttat
     9241 aaggccctgt cgacctttgc ttggcgagga attgacttga caaaaattga aagtcgtcca
     9301 ctcaagacag cactgggtga atactttttc attatcgatg tggattatac cgataaggac
     9361 ttggtccact ttgcccaaaa agaattagaa gcgattggaa tccagtataa aattctgggt
     9421 gcctatccta tttatccaat atcagaccat ggaaaggaga gaagatgagt aaagaaaatc
     9481 ccttaagtca tcatgagcag ttgcgttatg attatttgct aaaaaatatt cactatctca
     9541 atgagagaga aaaaaatgag tttgcctatt tgcaagaaaa gctaactctt gctaggggaa
     9601 atagtagctc tagcttggaa caagaaagag aagagcaggt tgacttacca agctatgcga
     9661 accggagtcg ctcacaatcc aaatcacaag cactctcttc ccctccaaaa aagaaaagac
     9721 ggaagctccg tcttaaacga atttttatgg tgattttttc acttttggtc tgtgtggctt
     9781 tggccatggt attcatgttt ttgcgtggtt accaagatgc tagcgcaaag aaaactgctg
     9841 atgcccgggc agctcaagta gaagtcttta atggtcagga cactagagat ggagttaata
     9901 ttttaatcat gggtactgat ggtcgaatcg gccagaacag tgttgagaca cgaactgact
     9961 ctattatggt attaaatgtc gggggctcag ataagaaaat gaagctagtc agtttcatgc
    10021 gtgataattt ggtctatata gatggttata gtcaagtgat taacggtaga aaacagacag
    10081 ataacaagtt aaacgtagcc tacgagttag gagaacaaga ggggcaaaaa ggggcagaaa
    10141 tggttcgcca agtcttgaaa gataattttg acttggacat taagtactac gccttggttg
    10201 atttccaggc ctttgccaca gcgattgaca cgcttttccc tgatggggtg acgattgatg
    10261 ctcaattttc aacattgaat gggcgtcccc taacagaagc tacagtcgga gatgatttat
    10321 acgctaccga gactgagtct ccaacccaaa ccattaaagt cggaaaacag cagatgaatg
    10381 gctcgaccct gctcaattat gctcgtttcc gtgatgatga tgaggcggat tacggccgta
    10441 caaaaagaca gcaacaagtt ttaacagcaa ttttagagca aattaaagat cccactaaac
    10501 ttttcactgg ttctgaagct cttggaaagg tttttgctat gacctcaacc aacgtaccct
    10561 atactttcct cctaacaaac ggtttatctg ttttggatgg agcaaaaaat ggtattgaaa
    10621 aattgacgat tccagaactg ggtgactggg tagatgccta tgatgtttat ggaggcttgg
    10681 gcttgctggt tgatcaaaac aaatatcaaa ccaagctcgc tcaaatgggc ttgagataag
    10741 ataatactca atgaaaatca aagtgcaaac taggaagcta gccgcaggct gctaaaaaca
    10801 cagttttgag gttgtagata agactgacga agtcagttac ctatacctac ggcaaggtga
    10861 agctgacgtg gtttgaagag attttcgaag agtataagac agaaaaatca aagtttgata
    10921 gctatttgga tagcagtcag gctttgattt tttacagtct gcaatagatt ttcacccctt
    10981 gtttgtggta taatgaaacg atacgataga aagaataatg aacaatatga ctgatttaaa
    11041 agcaattcag gctcgtagtc tggagatggc tgaatatttt gtggcctttt gtaaagaaca
    11101 tgatttgctc tgttatctct gtggcggagg cgctattggt gcccttcgaa acaagggatt
    11161 tattccttgg gacgacgacc tagacttttt tatgcctcgt aaagattatg agaaattagc
    11221 agaattatgg cctcgttatg cagatgaacg ttatttcttg tcaaagagtc acaaggattt
    11281 tgttgatcgt aatcttttta ttaccattcg tgacaaggaa accacctgta tcaagcctta
    11341 tcagcaggat ttggatttgc cacatggtct ggccttggat gttttgcctt tggattatta
    11401 tccgaaaaat ccagctgagc ggaaaaaaca ggttcgttgg gccttgattt attcactctt
    11461 ttgtgcgcaa actattccag aaaagcatgg tgctctcatg aaatggggaa gtcgcatttt
    11521 actgggtttg actccaaaat cactctgtta tcgcatctgg aaaaaagctg agaaagaaat
    11581 gactaagtat gatttggctg attgtgatgg cattacagaa ttatgctcag gtcctggcta
    11641 catgagaaac aagtacccaa tcacatcttt tgaagacaat cttttcttgc catttgaagg
    11701 aacagagatg cctattccaa tcggctatga tgtctatctc agaactgctt ttggggatta
    11761 tatgacgcct ccaccagcag acaagcaggt accgcatcat gatgctgtca tcgctgatat
    11821 ggataagtct tatacagaat acaagggaga atatggtggc taagaaaaaa atcttatttt
    11881 ttatgtggtc tttttctctt ggaggtggtg cagagaagat tctatcaacc attgtttcaa
    11941 atctggatcc agaaaagtat gatattgata ttcttgaaat ggagcacttt gacaagggat
    12001 atgaatctgt tccaaagcat gtacgcattt taaaatccct tcaagattat cgccaaacca
    12061 gatggttacg agcttttttg tggagaatga gaatttattt tccaagactg gctcgtcgtt
    12121 tgcttgtaaa agatgattat gatgttgaag tttcttttac cattatgaat ccaccactgt
    12181 tgttctctaa aagaagagaa gtcaagaaga tatcttggat tcatggaagt attgaagaac
    12241 ttcttaagga tagctctaaa agagaatcac atagaagcca gttggatgct gcgaatacaa
    12301 ttgtagggat ttcaaaaaag accagcaatt ctatcaagga agtttatcca gattatactt
    12361 ctaaattaca gacaatctac aatggatatg attttcagac tattctagaa aaatctcaag
    12421 agaagatcga tatcgagatt gctcctcaaa gtatctgtac tatcggacgg attgaggaaa
    12481 ataagggttc tgaccgtgta gtggaagtga tacgattatt acaccaagag ggaaaaaact
    12541 atcatctcta ttttatcggg gctggtgata tggaagagga actgaaaaaa cgagtcaaag
    12601 agtatgggat tgaggactat gtacatttcc ttggttatca aaaaaatcct tatcagtatc
    12661 tatctcagac gaaagttctt ttgtctatgt ctaaacaaga aggttttcct ggagtgtatg
    12721 tggaggcctt gagtctggga ctccctttta tctctacgaa cgttggaggg gctgaggaat
    12781 tatcccaaga aggacgattt ggacaaatca ttgagagcaa tcaagaggca gctcaggcga
    12841 ttactaatta catgacttct gcctcaaact ttgatgtcga tgaggctagc caattcattc
    12901 aacaatttac aattacaaaa caaatcgaac aagtagaaaa actattagag gagtagcatg
    12961 gaaactgcat taattagtgt gattgtgcca gtttataatg tggcgcagta cctagaaaaa
    13021 tcgatagctt ccattcagaa gcagacctat caaaatctgg aaattattct tgttgatgat
    13081 ggtgcaacag atgaaagtgg tcgcttgtgt gattcaatcg ctgaacaaga tgacagggtg
    13141 tcagtgcttc ataaaaagaa cgaaggattg tcgcaagcac gaaatgatgg gatgaagcag
    13201 gctcacgggg attatctgat ttttattgac tcagatgatt atatccatcc agaaatgatt
    13261 cagagcttat atgagcaatt agttcaagaa gatgcggatg tttcaagctg tggtgtcatg
    13321 aatgtctatg ctaatgatga aagcccacag tcagccaatc aggatgacta ttttgtctgt
    13381 gattctcaaa catttctaaa ggaatacctc ataggtgaaa aaatacctgg gacgatttgc
    13441 aataagctaa tcaagagaca gattgcaact gccctatcct ttcctaaggg gttgatttac
    13501 gaagatgcct attaccattt tgatttaatc aagttggcca agaagtatgt ggttaatact
    13561 aaaccctatt attactattt ccatagaggg gatagtatta cgaccaaacc ctatgcagag
    13621 aaggatttag cctatattga tatctaccaa aagttttata atgaagttgt gaaaaactat
    13681 cctgacttga aagaggtcgc ttttttcaga ttggcctatg cccacttctt tattctggat
    13741 aagatgttgc tagatgatca gtataaacag tttgaagcct attctcagat tcatcgtttt
    13801 ttaaaaggcc atgcctttgc tatttctagg aatccaattt tccgtaaggg gagaagaatt
    13861 agtgctttgg ccctattcat aaatatttcc ttatatcgat tcttattact gaaaaatatt
    13921 gaaaaatcta aaaaattaca ttagaacggg ggtgaccaag tgattgatgg gaaacgatta
    13981 ttatttagtt tgaccatagt cagttatgcc ttgacgctag taagtggaat tgtgtatctg
    14041 tttaataata ataatgttag cttactttct actttattgt tcttgttggt tagtagctta
    14101 attgcttgtt ggaatgatat caagtattac ttaatccatt ttattttcta tttaaccatt
    14161 tttgtatttc tggtatcaag accgaccatt gattatttta gggatggtgc tttggatacc
    14221 tatcatccaa tagcctatcg ttttgccttt atagttgtca tggtttcgat tctgggcttg
    14281 accacaggag gcattctggc tcgttacttc atagctagga agaaaataaa agtagcaaat
    14341 ataggaaatt ctctaaaaga ggtttatatc aagcggttac gctttgtatc actaggagtt
    14401 tttcttctaa cctatccttt ctatttcatt cggttatttg aacggctctt gtatcgtttg
    14461 cagacttcct actatgccta ctatgcaaat tttgaaagta aactgcctta ttttacctac
    14521 attttgtcta cctttacggt ctatgcaatg tgtatgtatc tggcaaccaa gccaaagaaa
    14581 ttgcaggcca cagcagtgct tgtctccttt attgcagcta atactattca tttggcaatt
    14641 gggacacgaa atccctttat tttaagtatt ttatttgctt ttgtttatta ctttatgcgg
    14701 gagcaaactg aaaaaggaaa atggattggg tttaaagaaa agttagcgat ttttgtaggt
    14761 tctcctattc tcatgttagc gatgggagta ctcaattatg tacgggataa tgtccaagtt
    14821 tctcatacag gtttctggga tatcttactt gactttatct ataaacaagg gactagtttt
    14881 ggtgttctgg ctcgaggttt tctatttaac agtagcctcc cttaccgaga tttccgtaat
    14941 tttacttttg gtcctgttct tgattatttt gcaaggggga gtttgggagc cattttcgga
    15001 ggaaaagcct ttgaacatac aaccaatagt gtggaactag ctattgatag taatagttat
    15061 gcccacaatc tatcctatct tgtcttgaac aaggaatact tgaaagggca tggtatcgga
    15121 agtagttata tcatggagtt gtataccgac tatggtatga ttggagtctt tctgcttagt
    15181 ttcttactcg gcgtattatt tatagccatg ctgcaagtag cctatcgctc aaggacaatc
    15241 ctatttgctt tatccctact catcttgaat aatctattct ttatgccaag aagcagcttt
    15301 tcagaaagtt tcttcaattt atttacaatg caattctggg gaattgttct tgtgattata
    15361 tttgtagcaa aaatgcttac aaaggaaaac tagtatctac taaacaaagg agaaaaaaat
    15421 catgtttgaa cattattcag tagctgattt gtttgcaaat ctttataaaa agcgaaaagc
    15481 aaatatttta gctctcatcg ctttatttgc tctcattgct gtaccattta caattaaagc
    15541 agttaggaat aaaaacactg tcaaagatac aacaagttat tcaacttatc ttatctataa
    15601 aatcactcct ccaaaagagt cggccaaaac gattttgaat catcaaattg gtggttatag
    15661 tgatttttat gggaaattga ttgatggtaa tttgaatgga gcttatcttt tcaatgatgt
    15721 agaacccagt gagttgaaaa aaattgccag tgaattagac acgacagaaa caaccttgaa
    15781 aaattcaacg aatgactatt ggtggaaaaa attgaccgtc tactatatga ttgacgatgc
    15841 aggggttggt gtgaaaattt tgacatcaag taaagatgcc aatgacttgt tagagaaaaa
    15901 aattgatggg ttgattgaga aatttaaaca tgcctatgca aatgtgaaaa ttgaaaaact
    15961 ggaaaccatc aactctaaag aattgaacgc aaatggtgaa acagcgcttg gcttaaatgt
    16021 gaaaaatctg attcttcgtt tagttgttat tggagtggtt tgtgtgattt tggttgtgat
    16081 gggaaatgtg ttagtttatc tctttaatcc aacaatcaat agagtaggtg atttttctca
    16141 gtatcaaatt gattttgtaa cagagattac aacaattgct aacctagcag atgttttgtc
    16201 atacaaaaat actggacagg aattgaccat cgttagctca aataaagcta tcctagataa
    16261 attgaaacag agtcaagaag ctttaaaagg aatgcatttt gtagatttac aggatgtatc
    16321 atctcttttg gaaagagata cagtccttct tgttgaagag tacggagtga ctcgttataa
    16381 gaaatttgag caaagtcttc aaattctcag aaacttaaat cgttctatcc ttggtgtagc
    16441 aacctttaaa ttataagtat tctgatgtat aaggtcttca aaatcggcta agagctgatt
    16501 ttgaaggctt tttctatctg tctgcagaaa ttttcttttg gagtctccta tagaaagacc
    16561 ctagggaaaa tctatccgta tagaggagcc ttcttttgat aaaatcgtaa aaatctagta
    16621 taatagatag aactgaaagt atgaggttac tagcaatgaa aatgaaacaa attagtgata
    16681 caactttaaa aatcacgatg tctttagagg atttgatgga tcgtggaatg gagattgctg
    16741 actttctcgt tcctcaagaa aaaacagaag agttctttta tgctatcttg gatgagctag
    16801 agatgcctga tagctttctg gatacaggca tgttgagctt ccgtgtgact ccaaaacctg
    16861 ataaggtaga tgtctttgtg accaagtcaa agattgacca aaatctagat tttgaagact
    16921 tatcggattt gccagatatg gaagaattgg ctcaaatgtc tccagatgaa tttatcaaaa
    16981 ccctggaaaa aagcatcgca gacaaaacca aggatgatat cgaagccatt caatctcttg
    17041 agcaagttga agccaaggaa gaagagcaag agcaggctga acaagaagct gagagtaaga
    17101 aagaacctta catctactac atcctttctt ttgctaagtt ggctgacttg gtagtttttg
    17161 ccaagacagt gacttttgaa atggaaactt ctgaactcta caaaatgaac gagcgctatt
    17221 atttgaccat tttagtggat attgaaaatc atccaagccc atatccagct tggctgttgg
    17281 cccgtatgcg cgagtttgca gacgatagtg atatcagtcg ctcagtctta caagagtatg
    17341 gtcaagtctt gatgagtcac gatgcagtgc tcaatctgca aaaaatcggc taatcctttc
    17401 tggaaagcta ttttcagatt ttaagaagag cgaatcgtct gattcgtttt ttctttttat
    17461 actgaaatag tgatttacta taataggaat tttcacaaaa ttctgttata atggctatat
    17521 tagaaaattt cgaggagata aagatgacag ttaaaattgc tttactagga tttggtaccg
    17581 ttgcaagtgg tgtgcctttc ctcctaaagg aaaatggagg aaaaatcaat caatcagcac
    17641 attcagatat caaagttgct aaggtattgg tcaaggatga agatgaaaaa aatcgcttgc
    17701 ttgcagcagg gaatgacttt aactttgtaa ccaatgtgga tgatatttta tcagaccatg
    17761 atattactat cgtagtggaa ttgatggggc gtattgagcc tgctaaaacc tttatcactc
    17821 gtgccttgga agctggaaaa cacgttgtta ctgctaacaa ggacctttta gctgtccatg
    17881 gcgcagaatt gctagaaatc gctcaagcta acaaggtagc actttactac gaagcagcag
    17941 ttgctggtgg gattccaatt cttcgtactt tagcaaattc cttggcttct gataaaatta
    18001 cgcgcgtgct tggagtagtc aacggaactt ccaacttcat ggtgaccaag atggtggaag
    18061 aaggctggtc ttacgatgat gctcttgcgg aagcacaacg tctaggattt acagaaagcg
    18121 atccgacgaa tgacgtagat gggattgatg cagcctacaa gatggttatt ttgagtcaat
    18181 ttgcctttgg catgaagatt gcctttgatg atgtagccca caagggaatc cgcaatatca
    18241 caccagaaga cgtagctgta gctcaagagc ttggttacgt agtgaaattg gttggttcta
    18301 ttgaggaaac ttcttcaggt attgctgcag aagtgactcc aaccttccta cctaaagcgc
    18361 acccacttgc tagtgtgaat ggcgtaatga acgctgtctt tgtagaatct atcggtattg
    18421 gtgagtctat gtactacgga ccaggtgcgg gtcaaaaacc aactgcaaca agtgttgtag
    18481 ctgatattgt ccgtatcgtt cgtcgtttga atgatggtac tattggcaaa gacttcaacg
    18541 aatatagccg tgacttggtc ttggcaaatc ctgaagatgt caaagcaaac tactatttct
    18601 caatcttggc tctagactca aaaggtcagg tcttgaagtt ggctgaaatc ttcaatgctc
    18661 aagatatttc ctttaagcaa atccttcaag atggcaaaga gggtgacaag gcgcgtgtcg
    18721 ttatcatcac acacaagatt aataaagccc agcttgaaaa tgtctcagct gaattgaaga
    18781 aggtttcaga attcgacctc ttgaatacct tcaaggtgct aggagaataa gatgaagatt
    18841 attgtacctg caaccagtgc caatatcggg ccaggttttg actcggtcgg tgtagctgta
    18901 accaagtatc ttcaaattga ggtctgcgaa gaacgagatg agtggctgat tgaacaccag
    18961 attggcaaat ggattccaca tgatgagcgt aatctcttgc tcaaaatcgc tttgcaaatt
    19021 gtaccagact tgcaaccaag acgcttgaaa atgaccagtg atgtcccttt ggcgcgcggt
    19081 ttgggttctt ccagctcggt tatcgttgct gggattgaac tagccaacca actgggtcaa
    19141 ctcaacttat cagaccatga aaaattgcag ttagcgacca agattgaagg gcatcctgac
    19201 aatgtggctc cagccattta tggtaatctc gttattgcaa gttctgttga agggcaagtc
    19261 tctgctatcg tagcagactt tccagagtgt gattttctag cttacattcc aaactatgaa
    19321 ttacgtactc gcgacagccg tagtgtcttg cctaaaaaat tgtcttataa ggaagctgtt
    19381 gctgcaagtt ctatcgccaa tgtagcggtt gctgccttgt tggcaggaga catggtgacc
    19441 gctgggcaag caatcgaggg agacctcttc catgagcgct atcgtcagga cttggtaaga
    19501 gaatttgcga tgattaagca agtgaccaaa gaaaatgggg cctatgcaac ctacctttct
    19561 ggtgctgggc cgacagttat ggttctggct tctcatgaca agatgccaac aattaaggca
    19621 gaattggaaa agcaaccttt caaaggaaaa ctgcatgact tgagagttga tacccaaggt
    19681 gtccgtgtag aagcaaaata aagaatagaa gataggatgg ggaaactctt gaccagaggg
    19741 tttcatatcc tttttgtgaa aagaagttta tactcaatga aaatcaaaga gcaaactagg
    19801 aagctagccg caggctgctc aaaacagtgt tttgaggttg tggatagaac tgacgaagtc
    19861 agctcaagac actgttttga ggttgcagat agaactgacg aagtcagtaa catatatacg
    19921 gtaaggtgac gctaacgtgg tttgaagaga ttttcgaaga gtattagtta aaaacgtgat
    19981 aaaggagaaa taaagatggc agaaatttat ctagcaggtg gttgtttttg gggcctagag
    20041 gaatattttt cacgcatttc tggagtgcta gaaaccagtg ttggctacgc taatggtcaa
    20101 gtcgaaacga ccaattacca gttgatcaag gaaacagacc atgcagaaac ggtccaagtg
    20161 atttacgatg agaaggaagt gtcactcaga gagattttac tttattattt ccgagttatc
    20221 gatcctctat ctatcaatca acaagggaat gaccgtggcc gccaatatcg aactgggatt
    20281 tattatcagg atgaagcaga tttgccagct atctacacag tggtgcagga gcaggaacgc
    20341 atgctgggtc gaaagattgc agtagaagtg gagcaattac gccactacat tctggctgaa
    20401 gactaccacc aagactatct caggaagaat ccttcaggtt actgtcatat cgatgtgacc
    20461 gatgctgata agccattgat tgatgcagca aactatgaaa agcctagtca agaggtgttg
    20521 aaggccagtc tatctgaaga gtcttatcgt gtcacacaag aagctgctac agaggctcca
    20581 tttaccaatg cctatgacca aacctttgaa gaggggattt atgtagatat tacgacaggt
    20641 gagccactct tttttgccaa ggataagttt gcttcaggtt gtggttggcc aagttttagc
    20701 cgtccgattt ccaaagagtt gattcattat tacaaggatc tgagccatgg aatggagcga
    20761 attgaagttc gttctcgttc aggcagtgct cacttgggtc atgttttcac agatggaccg
    20821 cgggagttag gcggcctccg ttactgtatc aattctgctt ctttacgctt tgtggccaag
    20881 gatgagatgg aaaaagcagg atatggctat ctattgcctt acttaaacaa ataaaacaga
    20941 gagtggggct tcccactttc ttcatttcta gaatatgaat agaagggatt tatgaaacac
    21001 ctattatctt acttcaaacc ctacatcaag gaatcaattt tagccccctt gttcaagctg
    21061 ttagaagctg tttttgagct cttggttccc atggtgattg ctgggattgt tgaccaatct
    21121 ttacctcagg gagatcaagg tcatctctgg atgcagattg gcctgctcct tatctttgca
    21181 gtaattggcg ttttagtggc cttgatagct caattttact cagcaaaggc agcagtaggt
    21241 tttgctaagg aattgacaaa cgatctttat cgtcatattc tttccttgcc aaaggacagc
    21301 agagaccgtc tgacaacttc tagtttggtt actcgcttga cttcggatac ctaccagatt
    21361 cagactggta tcaatcaatt cctgcgtctc tttttacgag cgcccattat cgtttttggt
    21421 gccattttta tggcttatcg aatctcagct gagttgactt tctggttctt agtcatggtt
    21481 gccattttga ccattgtcat tgtagggtta tctcgattgg tcaatcctct ctacagtagt
    21541 ctcagaaaga aaacggacca actggttcag gaaacgcgcc agcaattgca agggatgcgg
    21601 gttattcgtg cttttggtca agaaaaacga gagttacaga tttttcaaac ccttaaccaa
    21661 gtttatgcta gattacaaga aaagacaggt ttctggtcta gtttattaac acctctgacc
    21721 tatctgattg tcaatggaac tcttctcgtt attatctggc aaggctatat ttcaattcaa
    21781 ggaggagtgc tcagtcaagg tgctctcatt gctcttatca attacctctt acagattttg
    21841 gtggaattgg tcaagctagc catgttgata aattctctca accagtccta tatctcagcc
    21901 aagcgaatcg aggaagtctt tgttgaggct ccagaggata tccattcaga gttagaacaa
    21961 aagcaagcta ccagtgatcg ggttttacaa gtccaagaat tgacctttac ctatcctgat
    22021 gcagcccagc cttctctgag agacatttcc tttgatatga ctcaaggaca aatccttggt
    22081 atcattgggg ggactggttc tggtaaatca agcttggtgc aactcttact tggactttat
    22141 ccagtagaca aggggaacat tgacctttat caaaatggac gtagtcctct taatttggag
    22201 cagtggcggt cttggattgc ctatgtacct caaaaggtcg aactctttaa gggaactatt
    22261 cgttccaact tgactctagg tttaaatcaa gaagtgactg atcaagaact ctggcaagcc
    22321 ttggaaattg ctcaagctaa ggattttgtc agtgataagg aaggactctt ggatgctcta
    22381 gttgaagcag gagggcgaaa tttctcaggt ggacaaaaac aaaggctgtc tatcgcacga
    22441 gcagtcttgc gccaggctcc gtttctcatc ctagatgatg cgacctcagc actggacacc
    22501 attacagagt ccaagctctt gaaagctatt agagaaaatt ttccaaacat gagcttaatt
    22561 ttgatctctc aacgaacctc gactttacag atggcggacc agattctcct cttggaaaaa
    22621 ggtgagttgc tagctgttgg caagcacgat gacttgatga aatccagcca agtctatcgt
    22681 gaaatcaatg catcccaaca tggaaaggag gactagaatg aaacgacaaa ctgtaaacca
    22741 gacgctcaaa cgtttagccg tagatttagc aaaccatccc ttcctccttt tcctagcctt
    22801 tctaggaact attgcccaag ttggcttatc aatttaccta cctattctga ttgggcgggt
    22861 cattgaccaa gtcctagtgg ctggttcatc accagttttt tggcagattt ttctccagat
    22921 gctcttggtg gtaataggaa atactctagt acaatgggcc aatcctctcc tctataatcg
    22981 tctaatcttc tcttatacca gagatttacg ggagcgaatc atccataaac tccatcggtt
    23041 accgattgcc tttgtagata ggcaaggtag tggagagatg gttagtcgtg taaccacaga
    23101 catcgaacag ttggcagctg gcttgaccat gatttttaac caatttttca ttggtgtttt
    23161 gatgattttg gttagtattc tagccatgct ccaaattcat ctcctcatga ctctcttagt
    23221 cttgctgttg acgccactgt ccatggtgat ttcacgcttt attgccaaga gatcctatca
    23281 tctcttccag aagcaaacag agacgagggg gattcagacc cagttgattg aagaatcgct
    23341 tagccagcag actataatcc agtccttcaa tgctcaaaca gagtttatcc aaagattgcg
    23401 tgaggctcat gacaactact caggctattc tcagtcagcc atcttttatt cttcaacggt
    23461 caatccttcg actcgctttg taaatgcact catttatgcc cttttagctg gagtaggagc
    23521 ttatcgtatc atgatgggtt caaccttgac cgtcggtcgt ttagtgactt ttttgaacta
    23581 tgttcagcaa tacaccaagc cctttaacga tatttcttca gtgctagctg agttgcaaag
    23641 tgctctggct tgcgtagagc gtatctatgg agtcttagat agccctgaag tggctgaaac
    23701 aggtaaggaa gtcttgacca gtgaccaagt taagggagct atttccttta aacatgtctc
    23761 ttttggctac catcctgaaa aaattttgat taaggacttg tctatcgata ttccagctgg
    23821 tagtaaggta gccatcgttg gtccgacagg tgctggaaaa tcaactctta tcaatctcct
    23881 tatgcgtttt tatcccatta gctcgggaga tatcttgctg gatgggcaat ccatttatga
    23941 ttatacacga gtatcattga gacagcagtt tggtatggtg cttcaagaaa cctggctcac
    24001 acaagggacc attcatgata atattgcctt tggcaatcct gaagccagtc gagagcaagt
    24061 aattgctgct gccaaagcag ctaatgcaga ctttttcatc caacagtttc cacagggata
    24121 cgataccaag ttggaaaatg ctggagaatc tctctctgtc ggccaagctc agctcttgac
    24181 catagcccga gtctttctgg ctattccaaa gattcttatc ttagacgagg caacttcttc
    24241 cattgataca cggacagaag tgctggtaca ggatgccttt gcaaaactca tgaagggccg
    24301 cacaagcttc atcattgctc accgtttgtc aaccattcag gatgcggatt taattcttgt
    24361 cttagtagat ggtgatattg ttgaatatgg taaccatcaa gaactcatgg atagaaaggg
    24421 taagtattac caaatgcaaa aagctgcggc ttttagttct gaataagcca ttctcttttg
    24481 aaagtttctg gacgaaaaaa gttgccttcg agtgactttt ttgttacaat agctagaaaa
    24541 attgttcact gtaatactca atgaaaatca aagagcaaac taggaagcta gccgtaggtt
    24601 gctcaaagca cagctttgag gttgtagata agactgacga agtcagctca aagcactgtt
    24661 ttgaggttgc agatagaact gacgaagtca gctcaaaaca ctgttttgag gttgcagata
    24721 gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact gacgaagtca
    24781 gtaaccacac ctacggcaaa gtgaatctga agtggtttga agagagtaca acttgtcttt
    24841 tagaaaagga gcctataatg aaagtctttc agcatgtaaa tatcgtgact tgtgatcaag
    24901 atttccatgt ttatcttgat ggaatcttag cagtcaagga ttctcaaatc gtctatgtcg
    24961 gtcaagataa gccagcgttt ttagagcaag ctgagcagat tatagactat cagggagctt
    25021 ggattatgcc tggtttggtc aattgtcaca cccattctgc aatgacaggt ctgagaggga
    25081 tccgagatga cagcaatctc catgaatggc tcaatgacta tatctggcca gcagaatctg
    25141 agtttactcc cgacatgact accaatgcgg tcaaagaagc cctgacagag atgctccagt
    25201 caggaacaac aacctttaac gatatgtata atcccaatgg tgtggatatc cagcaaattt
    25261 atcaggtggt gaaaacttcc aagatgcgtt gttatttttc tccgactctc ttttcttcag
    25321 agacagaaac aactgctgag actataagca gaactcgatc catcatagac gaaatcttaa
    25381 aatataaaaa tccaaatttc aaggttatgg tagcacctca ttctccgtat agctgcagta
    25441 gagacttgct ggaagcgagt ttggaaatgg caaaagagct aaatattccg ctccatgtcc
    25501 atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa acgatacggc aaacgccccc
    25561 ttgcttttct tgaagaactg ggttatttag atcatccgtc cgtatttgct cacggggtcg
    25621 aattaaacga gagagaaatt gaacgcttgg catcttctca agtggctatc gcccacaatc
    25681 ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat tatccagctc caaaaagcgg
    25741 gagtagcagt cggaattgcg actgactcgg ttgcttccaa taacaatcta gatatgtttg
    25801 aggaaggaag gactgcagct cttcttcaga agatgaaaag tggggatgcc agccagtttc
    25861 caatcgaaac agctctcaag gtactgacaa tcgaaggggc taaggtcctt ggaatggaaa
    25921 atcagatagg aagtctggaa gtcggcaagc aagcagattt tctggtcatt caaccacaag
    25981 ggaaaattca tctccaacct caggaaaata tgctgtctca cctggtttat gcagttaaat
    26041 ctaatgatgt agatgatgtt tatatcgccg gagaacaggt tgttaagcaa ggtcaagtcc
    26101 tgacagtaga actttaaaag aaaaat
//
LOCUS       DAVLWA010000021        25323 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLWA010000021 DAVLWA010000000
VERSION     DAVLWA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.021"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(103..1737)
                     /locus_tag="VYN80_000853"
     CDS             complement(103..1737)
                     /locus_tag="VYN80_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540791.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLVNLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSTFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1718..2467)
                     /locus_tag="VYN80_000854"
     CDS             complement(1718..2467)
                     /locus_tag="VYN80_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540792.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPAQSLESIYLSLAGRKEEVADASQGH"
     gene            2646..4100
                     /locus_tag="VYN80_000855"
     CDS             2646..4100
                     /locus_tag="VYN80_000855"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1540793.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4190..6808)
                     /gene="alaS"
                     /locus_tag="VYN80_000856"
     CDS             complement(4190..6808)
                     /gene="alaS"
                     /locus_tag="VYN80_000856"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1540794.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6830..7315)
                     /locus_tag="VYN80_000857"
     CDS             complement(6830..7315)
                     /locus_tag="VYN80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1540795.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7590..7829)
                     /locus_tag="VYN80_000858"
     CDS             complement(7590..7829)
                     /locus_tag="VYN80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540796.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8082..9152)
                     /locus_tag="VYN80_000859"
     CDS             complement(8082..9152)
                     /locus_tag="VYN80_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540797.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9149..9922)
                     /locus_tag="VYN80_000860"
     CDS             complement(9149..9922)
                     /locus_tag="VYN80_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540798.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9919..10725)
                     /locus_tag="VYN80_000861"
     CDS             complement(9919..10725)
                     /locus_tag="VYN80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540799.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10706..11863)
                     /locus_tag="VYN80_000862"
     CDS             complement(10706..11863)
                     /locus_tag="VYN80_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540800.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12023..12928)
                     /gene="murB"
                     /locus_tag="VYN80_000863"
     CDS             complement(12023..12928)
                     /gene="murB"
                     /locus_tag="VYN80_000863"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1540801.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13040..13792)
                     /locus_tag="VYN80_000864"
     CDS             complement(13040..13792)
                     /locus_tag="VYN80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1540802.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13802..14533)
                     /gene="budA"
                     /locus_tag="VYN80_000865"
     CDS             complement(13802..14533)
                     /gene="budA"
                     /locus_tag="VYN80_000865"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1540803.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14675..15946
                     /locus_tag="VYN80_000866"
     CDS             14675..15946
                     /locus_tag="VYN80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1540804.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15966..16781)
                     /locus_tag="VYN80_000867"
     CDS             complement(15966..16781)
                     /locus_tag="VYN80_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1540805.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16938..17591)
                     /gene="phoU"
                     /locus_tag="VYN80_000868"
     CDS             complement(16938..17591)
                     /gene="phoU"
                     /locus_tag="VYN80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1540806.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17603..18361)
                     /gene="pstB"
                     /locus_tag="VYN80_000869"
     CDS             complement(17603..18361)
                     /gene="pstB"
                     /locus_tag="VYN80_000869"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1540807.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18374..19177)
                     /gene="pstB"
                     /locus_tag="VYN80_000870"
     CDS             complement(18374..19177)
                     /gene="pstB"
                     /locus_tag="VYN80_000870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1540808.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19188..20072)
                     /gene="pstA"
                     /locus_tag="VYN80_000871"
     CDS             complement(19188..20072)
                     /gene="pstA"
                     /locus_tag="VYN80_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1540809.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGIYLHKKLTAA"
     gene            complement(20062..20979)
                     /gene="pstC"
                     /locus_tag="VYN80_000872"
     CDS             complement(20062..20979)
                     /gene="pstC"
                     /locus_tag="VYN80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1540810.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGIWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20979..21857)
                     /gene="pstS"
                     /locus_tag="VYN80_000873"
     CDS             complement(20979..21857)
                     /gene="pstS"
                     /locus_tag="VYN80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1540811.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22431..23735)
                     /locus_tag="VYN80_000874"
     CDS             complement(22431..23735)
                     /locus_tag="VYN80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1540812.1"
                     /translation="MQFPEGFVEKYKEILGDEARNFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23736..24509)
                     /locus_tag="VYN80_000875"
     CDS             complement(23736..24509)
                     /locus_tag="VYN80_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1540813.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24499..24777)
                     /locus_tag="VYN80_000876"
     CDS             complement(24499..24777)
                     /locus_tag="VYN80_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1540814.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24777..25178)
                     /locus_tag="VYN80_000877"
     CDS             complement(24777..25178)
                     /locus_tag="VYN80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1540815.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         7582 a         5805 c         4903 g         7033 t
ORIGIN      
        1 ctatatattt ttgattacca atcttaatca tttacaacta cattctaaca aactataaaa
       61 gcgtttgtcg aattgaattt atcaagcaag cgaccaacca gctcatcttt tttctatttc
      121 tgccaatatg cgtgacaggt aataatgata gccaaaaata gcaagagcaa gcaggaggat
      181 aagagctcct actcccaagt tgatggcaag gataggggag agagactgaa ccaagaatat
      241 gctcccaatt acaagggcca tcaggattgc actataaata aacaataaaa ctatggcgac
      301 tatgccattt gaacgattca ccaggtccgt aatgctactc caattggttg acagattttt
      361 aacgtcctta aagtaatggt ggcaagaaag gatgacactg gcaatgatcc agactacaag
      421 aaggtaaatc atcgaaagga tggacaagcc tagatataga gaaagaccga gcaaagtcag
      481 aactggtaaa aaggactgaa cagcaaagat aatccaaaat ttcactttca cataacgagc
      541 aaagtcaaag ggtaaactct tcagaaaatc aacattttcc ctctccaagg acaaggcaat
      601 tgaatgcagg ctggtgatat tgttattgac aactgctata aacactccta caaaaaacaa
      661 gggtaaccag tatggaggat gaatgtctgg aactatctga gaatctcgga ttttggaaat
      721 cagactgatc atcatgagat aaggaaggaa agtacttgta aaaagcactg taatcacgcc
      781 agtcccctgt cccaagaggg tgaggtggta gcgtaaaacc atgcggaaaa atcccttttt
      841 agtagttgaa atcctctcct tgctgcgacg ttcttttttg accttctcct cactgttaag
      901 caggatcacg tcataaaaac gaggaaggac cttctttttg gtcaggtaaa gcaagaaaac
      961 agttaaagct atccaagcga gcagacccac taaggcttct gttgaaaaag gctccactgc
     1021 tattttgtaa aagatatgaa gaggataaag gagaaatgga atgtctctaa ctttgtcaac
     1081 aatacttcca aaagtcgact gtagaaagaa gacaaatatt aaaggtatga gaactcctat
     1141 cccaatcatc acattcgaaa aaatagactg atactttctg aagaccctag tctgagccaa
     1201 gaaatgcact gccactacca tcactagagc cacagagaca aataataagg tcaaggacag
     1261 tagcatcaaa ggcaaaccca gccatagaga aggagctagc ctaatgtaga ggactagaaa
     1321 ataagctagg attggtacaa ttccagttag agctggcaag aggacagaca gtcctttagc
     1381 aattataatc tctgattctt taaaggcata gggcctatac gataccaaat ccttactctc
     1441 ataaaagaca ttgtaaaagg ccgttaaaga agttgaaaag gcaatcacta gtaaaatagc
     1501 aaccatcgaa ctaaaataaa taggcatttc ctcaaaagga aaatgaatgg ctatattact
     1561 aaaacagatg agcatcaaga gactggaaaa aatgtaagaa cttaggactc tagcggaaac
     1621 atttactttt ttcccaggat tcttagcctg cttctttcgt agattaacca gattagcttc
     1681 ttgagatgaa taaaggatat tgatatcaac taatttttta atgaccttga gacgcatccg
     1741 caacctcctc ttttctacca gcaagactaa ggtagatact ttccaaagac tgggctgggt
     1801 aatcttttct caagtcctct acactaccac aataaatcaa atgccccttt ttcaaaatgg
     1861 caatccgatc acagacttgc tctgccacct ctaggacatg agttgaaaac aagactgtct
     1921 tccctttttg tgcatgttcc ttcatcatct gtttcaaatc aaaggcagcc tggggatcca
     1981 aaccagtcaa gggttcatcc aagacccaaa tatcgggatc agacaagagt gctccgatga
     2041 caaagacttt ctgacgcatt ccgtgagaaa gagtttcaat aacctgatag cgattttcag
     2101 caaaatcaaa aacgttcaat agcctagcta gactagtctc caagtcagag ctagtcagat
     2161 cataggatga agcgatcaat tcccaaaatt cattggccgt taagcgtaaa aataagtcag
     2221 gcgagtctgc tacgtagcca atctttcgtt tgatcgccaa gcgattttcc gataactcct
     2281 gaccgtctac caaaatacga ccactgctgg gtgaaatgat actgactagg gattttatgg
     2341 tggtcgattt tccagcccca ttatgaccaa tcaagcccat aatctctcca ttttcaatct
     2401 gcaaattgag attactcaag gcctctttat caccatacaa cttactaaca ttttgaaatt
     2461 caatcatggg acaactccta tctttatctg tattatacat cgctatacta gcactttgat
     2521 acaaaaaaat caatacttta ttttaagtag ttaaaatagt ttccttctat cttacgcaaa
     2581 cgtttgcgcc gagagatact ttatgataga ataaagaaca agattgacaa gtaagaggaa
     2641 acatcatgca aaatcaaaca ctcatgcaat actttgaatg gtatctgccc cacgacggtc
     2701 aacactggac gcgtctggct gaaaatgctc cacacctagc tcatctgggg atcagtcacg
     2761 tctggatgcc accagccttc aaggcaacca acgaaaaaga tgtcggctat ggggtctatg
     2821 acttatttga cttaggagag ttcaaccaaa aagggactgt ccgcaccaag tatggtttca
     2881 aagaagacta tcttcaagcc attcaagccc ttaaagaaca gggaattcaa cctatggccg
     2941 atgtagttct caaccacaag gctgctgccg ataacaggga agcctttcag gttatcgaag
     3001 ttgatcctgt agaccgtaca gttgaacttg gagaaccctt caccatcaat ggctggacta
     3061 gttttacctt cgatggtcgc caagatacct ataatggctt ccactggcat tggtaccact
     3121 tcaccggtac agactacgat gccaaacgca gtaaatctgg gatttatctg atccaagggg
     3181 acaacaaggg ctgggctaac gaggaattgg tcgataacga aaacggaaac tacgactacc
     3241 tcatgtatgc cgacctagac tttaaacatc ctgaagtcat ccaaaacatc tatgactggg
     3301 ctgattggtt catggaaacg actggtgtag ctggtttccg tttggatgcc gttaagcata
     3361 ttgactcttt ctttatgcgc aacttcatcc gcgatatgaa ggaaaaatac ggtgacgatt
     3421 tctatgtttt tggtgaattt tggaacccag acaaggaagc caatctggac tatctcgaaa
     3481 aaacggaaga acactttgac cttgtcgatg ttcgtctcca ccagaatctc tttgaagcca
     3541 gtcaagctgg cgcaaactat gaccttcgtg gcattttcac agatagcctg gttgaactca
     3601 agcctgacaa ggctgtgact tttgtcgaca accacgatac ccaacgagga caagcccttg
     3661 agtctaccgt tgaagaatgg ttcaagccag cagcctatgc cctcattttg ttacgccaag
     3721 acggccttcc atgtgtcttt tacggagact actatgggat ttcaggccag tatgctcaag
     3781 aagatttcaa agaaatcctt gaccgcctcc tagccatccg aaaagacttg gcctatggag
     3841 aacaaaatga ctactttgac catgctaact gtatcggttg ggtacgttca ggtgctgaaa
     3901 atcaatcccc aatcgcagtc cttatctcaa atgaccaaga aaacagcaag tcaatgtttg
     3961 tcggtcaaga atggactaat caaacctttg tagatttact tggtaaccac caaggtcaag
     4021 ttacaattga tgaggaaggt tatggacaat tccctgtctc agctagatcc gtaagtgtct
     4081 gggcagtcaa taccatctaa tagctcataa taaccaagct aggtccaagc ggatttggct
     4141 tttttgtatt cacaaaaaga cctacccaaa tggatagatc tttacttgat tacaatttac
     4201 ctgctactgc atccaacaat tcttggatct tagcttggtt gcttcctcct gccatggcca
     4261 tgtctggttt accaccacca cgtccatcga tgattggtgc taattctttg acaaggtttc
     4321 ctgcatgaag gtcttttgtc ttgcttgcta caaggacatt gactttgtca ccgatagcgg
     4381 caactaggac aagaagatca gagtagtctt tttgtttcca gttatctgca aaagtacgaa
     4441 gggcaccggc atcggataca gacacttgac tagcaatgta acgatgaccg ttgacttcct
     4501 tagcatcttt gaagatatcg cctgcggctg cagctgcggc tttttctttc aactcagcat
     4561 tttctttttg aagttgacga agttgttctt gaagtccttc taccttgtga ggtacttcct
     4621 tgacttgagg tgctttcaag attgctgcga cagctttaag agcatcctct tgttcacgat
     4681 aggcttcaaa ggcttcctta ccagtcactg ccaagatacg gcgagttcct gaaccgattc
     4741 cttcttcttt gacaattttg aagagaccaa tctcagaagt gttgccaaca tgagtaccac
     4801 cacaaagttc aatagagtag tcaccgatag tcacgacacg aacttccttg ccgtatttct
     4861 caccaaagag ggccatagct cccatttctt tagcagtgtc aatatccgtt tcaactgtct
     4921 taacttcaag agcttcccag attttttcgt tgacttgctg ttcaatcgca cgcaattctt
     4981 cagcagttac agcttggaag tgggtaaagt caaagcgaag gaattcaact tcgttaagag
     5041 atcctgcctg tgttgcgtgg tttccaagga tattgtgaag ggcagcgtga agcaaatgag
     5101 tcgcagtgtg gtttttcatg acacggtgac ggcgattgct atcaattgcc aaggtatatt
     5161 cttggttcaa ggcaagcggt gcaaggactt caactgtatg aagggcttga ccatttgggg
     5221 ctttctgaac attggtcaca gtagccacaa ccttacctga ctcatccaag atttgtccgt
     5281 agtcagctac ctgtccaccc atttcagcat aaaatgacgt ttccgcaaag ataagagagg
     5341 cagttccttc tgaaacagct tctacttctg cattgtccgc cacgatagct accaatttag
     5401 aagacaattg gctagcattg tagttgaaga cactttctac agtgatgttt tgaagagttt
     5461 cattttgcat acccattgag ccacccttga cagctgaggc acgcgcgcgt tcttgctgtt
     5521 ctttcatggc tgcttcaaaa ccttcacggt ctacagtcat accagcttct tcagcgattt
     5581 cttcagtcaa ttcaactggg aacccataag tatcatagag tttgaagaca tctgaaccag
     5641 cgataacaga ttgacctttt tctttcaagt ctgctacaat gccttgggca aagtgttgac
     5701 ctgagtgaag ggtacgggca aatgattctt cttcgctctt aacgattttc tcaataaagt
     5761 cacgtttctc aagcacttct gggtagtagc tttccatgat ttttccaaca gttggaacca
     5821 atttgtaaag gaaaggttcg ttgataccca atttttgacc atgcatagaa gcacgacgga
     5881 gaagacgacg aagaacataa ccacgacctt catttcctgg aagggcacca tcaccgatag
     5941 caaatgaaag agaacgaatg tggtctgcga taaccttgaa gctcatgttg tcgccatctt
     6001 ggtcataaac cttaccagac aatttctcga cttcacggat aatcggcatg aagaggtccg
     6061 tttcaaagtt ggtcttagcc ccttggataa cagccaccaa acgctccaaa ccagcgcccg
     6121 tatcaatgtt cttatgtggc aattccttgt attcgctacg aggaacagca gggtctgcgt
     6181 taaattgtga caaaacgatg ttccagattt caatataacg gtcgttttca atatcttctg
     6241 caagcaggcg aagaccgata ttttctgggt caaaggcttc cccacggtca aagaagattt
     6301 ctgtatctgg tccagaaggt cccgcaccga tttcccagaa gttgtcctca attggaatca
     6361 agtgacttgg atccactccc acttcaatcc agcggttgta agaatcttta tcgtctggat
     6421 agtaggtcat gtaaagtttt tcagcaggga aatcaaacca ttcagggctt gtcaaaagct
     6481 cataagccca agtgatagct tcgtcacgga agtaatcccc gatagagaag ttccccagca
     6541 tttcaaacat ggtatggtga cgcgcagtct tccctacgtt ttcgatgtcg ttggtacgga
     6601 tagccttttg ggcattggta atacgtggat tttcagggat aatggtcccg tcaaagtatt
     6661 tcttaagggt tgctacccca gagttgatcc acaaaagagt tgggtcattt acaggaacca
     6721 aacttactga tggttctact gagtgagctt tggtcgccca gaaatcaagc cacatttggc
     6781 gtacttgtgc actagatagt tgtttcatat tgtctcctta tttacttgtt taatgtgatt
     6841 ggctttccag catttccaca tagtcaatcg cgacacagag ggaaatgact aggtctgcat
     6901 aagcgtcctc aagaaccgtt acggtatagg tagaggtcag atggaagagt tccttcttaa
     6961 tttccgcaat cagctgatcg cgatcatcca gcaatttgaa attcaaatcc cagatattgc
     7021 cctcgatacg aagacctaga ttatcaaact catacttatc tcgccagaag gtcaacttct
     7081 tacgaatgac aaaactcgag ccatcccgaa gctgaatctc aaaacgagga agcaaggtca
     7141 agatttcttt actaatctca ctgacttgtt caccagccgc atcatagatg gtaaaggttt
     7201 taggaatctt aaaaaatgat ccctccacct gataggcaat ttctcccctg tcattcttga
     7261 tagcgaagcg ttcgcctcca agacgaaact tttgtttgac aagaaatgtt ttcatcaaca
     7321 cctccaaaaa tcaaaagaca agctcatatc acgaagggcg aaaaaccgcg gtaccacctt
     7381 cattcaatga acttgtcatt ctcttgttct tatgcaattg tatgattgag tagcatgact
     7441 tcctagctta gatggctcgc agcaccgcca tttctctgga ctaagacaag tgaaaatcaa
     7501 ttctcaactt tcttattata acgttttttt aagcttgcgt caactggaaa tgatctccgt
     7561 tgaattagac caattcccta catctctgat tactttttca ggatatattt tttcttactg
     7621 ccgtttttct ttttatccca aattttcata ttactaaaca cagctactag aatatttcca
     7681 aatataaagg tgcctatcac ccaatatatg gactcagttg ttaggtattg tcgatccaag
     7741 ccatccttta aatggaatag tataacagtt tggttaacaa tcataaaggt tggccagaaa
     7801 ctttttttga aaaaagtaga cattttcatt atttgttgcc gctttctgta aggttaatac
     7861 tcaataaaaa tcaaaaagca aactaggaag ctagcctcaa gctgtacttg agtacggcaa
     7921 ggcaacgctg acgtggtttg aagagtatag gcttagtata ctactaggca agcaaataaa
     7981 caaataaaca actagaatag aaaaagatag ggctctaaaa actgacttct attccttaaa
     8041 aacgaaccag cttgactgat tcgtcttctt acgtttatct cctacttccg atacatttta
     8101 aactgtagga agaggtcgct atatttccct gtccatttat ggtcaaattt ctcataaact
     8161 tctaggtgtt tcatggtttc aacatcggga tagaaggcct tatcttcctt tgtttcctct
     8221 gggagcaatt ccttcgctgg taggtttggt gttgaatagc cgacatactc cgcattttgg
     8281 agagcatttt caggtttcaa cataaagttg ataaaggcat aggctgagtt ttggttttta
     8341 actgttttgg gaatgaccat attgtcaaac caaagattgc tggcctctgt cggtaccaca
     8401 taacgtagat tttcattttt ttctaacatt tggctggctt caccagagaa ggtcacgccg
     8461 attgcaacat tattctgaat catatagccc ttcatctcgt ccgcaacgat agccttgata
     8521 tttggagtca gtttgtagag cttatccact gtctcttcca actgctgcag atccttggag
     8581 ttgaggctgt agccgaggga attgagtcct agtcccagca cctcacgcgc cccatcaaag
     8641 agcatgatag aattcttata ctccggcttc caaaggtcat cccaatgctc aggcgcttca
     8701 tctaccatgg tttcgttgta gacaattcct aaggttcccc agaagtaagg gatggagaat
     8761 ttattacctg ggtcaaagga ctggttgaga aactctggtc cgatattttc gattccttta
     8821 atttttgaat aatcaagcgg aaccaagagg tcttcgtcct tcatcttgtt aatcatgtat
     8881 tcacttggaa tggcaatatc gtaggtcgtt ccaccctgct ttatcttagt gtacatggct
     8941 tcgttggagt caaaagtctc gtactgaact tgaattcctg tttcttctgt aaactgagtc
     9001 aagagttcag gatcgatata gtctccccag ttatagataa ccaatttttg actatctcga
     9061 ctattgattt tactatctaa atgagtcgca attccccaca agacaaggat aatcgctgca
     9121 attcctgcta aaaatgaata gatttttttc atgcttgctc ctccttctca cgagagataa
     9181 agtaataacc tacaactagg ataatactaa agagaaagac tagagcagac agggcattga
     9241 tttctaagga aatccccttg cgagcacgag agtaaatctc gactgatagg gttgaaaagc
     9301 catttcctgt tacaaagaag gtcacggcaa aatcatccag cgaataggtg aaggccatga
     9361 agtaacctgc aatgatagac ggagtcaggt aaggaagcat gatttcctta aacatctgaa
     9421 attgactagc tcccaagtca taggccgcat gaatcatgtc gccattcatt tccttgagtc
     9481 gaggcaagac catcaagacc acgataggaa tggagaaggc cacgtgacta gatagaacgg
     9541 tcaaaaagcc aagtgaaaac ttgagttggg taaagagaat caagaagcta gcaccaatca
     9601 taacgtcagg cgcaaccatg aggatattat tgagtgatag aaaggcttct tggtatttct
     9661 tacgagactg gtagatgtaa atggcaccaa aagtcccgat aatggtcgct atcaaggctg
     9721 ataggaaggc caagaaaaat gtctgagcca aaatcagcat gagtctccca tctccaaaca
     9781 tggtttcaaa gtgagtccag ctaaaacctg taaagctatt catatcatca ccagcattaa
     9841 aggcatagcc aatcaagtaa aaaatcggca ggtagaggac cagaaagacc agtcccagat
     9901 aaaggttggc aaattttttc atcgttctct cctttccttg gtcacccaca tggtgatgaa
     9961 catggtcagg atgagaatca caccgatggt tgaacccata ccatagttgt cattggttag
    10021 aaaattctgc tcaatagccg tccccaaggt gataacgcgg ttcccaccaa tcaaacgggt
    10081 cagcatgaag agactcaaac ttgggataaa gaccgactga accccacttc gcacaccgtt
    10141 catagataga gggaagatga catgacggaa ggtctcccac ttagttgcac caaggtcgta
    10201 actagcattg atgagattat tatccatatc gtctaagaca ttgaaaatcg gcaaaatcat
    10261 aaaggggagc tcgatgtagc ttgcgacaaa gataaaggaa aaatcggtaa aaagcaactg
    10321 ttgtgaacca attccgataa attccaagaa ttggttaata gagccatttt gaccaaaaat
    10381 cccgataaaa gcataggctt taaggagcaa attgatccag gtaggaagga taatcagcat
    10441 gagccagagt tgacggtgct tgagacgggt caaaaagagg gctgttggat agctgataag
    10501 caaggtcaca aaggtcacaa tccctgcata aagcactgag ttgaaactca ttttgaggta
    10561 ggtcaagttt tgtgacgcaa agtaagattt ataattttct aaactaaact ggccttcaat
    10621 gttgaaaaag gattgtccga aaatcaagac caagggtgcc aatacaaaga gggcaatcca
    10681 aagcatgtag ggcactacaa agagtttaga gcttgttttc ttcatctctt tcctcctcga
    10741 tcgcattgat cagacctgct tcttgctctt cgatttccac gtattcttcg atacgagcat
    10801 cgaactcttc ttcggtttca ttgagacgca tgatgtggat gtcttctgat tcaaagtcca
    10861 gaccgatttc ctcacccacg atggccttac gggttgagtg gatcatccat tcatttccaa
    10921 gttcgtcata ggcgataatt tcataatgaa ctccacggaa gagctgggtg tcgaccttaa
    10981 cttggagttt gccttcttca ggaagggtaa tgcgcaagtc ctctggacga ataacgacct
    11041 caacaggttc atttggcttc atcccaccat caaccgcttc aaagcgtttg ccgttaaatt
    11101 cgaccaagta gtcctcaatc atggtacctg gcaagatgtt tgactccccg ataaaggtgg
    11161 caacaaagtg gttgattggc tcatcgtaga tgtccacagg ggttccagac tggacaatct
    11221 cgccatcatt cataacgaaa atccagtcac tcatggcaag agcttcttcc tgatcgtgag
    11281 tgacaaagac aaaggtaatg cccaatcgtt gttgtaattc acgcaattcg tactgcatgt
    11341 ctgttctcaa tttcaagtcc agcgctgata aaggctcgtc caacaagacc acacggggtt
    11401 ggttgatgat agcacgggcg atggccacac gctgacgttg tcctccagaa agtttgcgga
    11461 tggaacgttt ttcataacct tccaactgaa ccatcttgag aacttccgct acacgctgct
    11521 cgatttcttt cttatcaatt ttacgcaagc gaagtggaaa ggcaacattt tcaaacacat
    11581 tcatatgtgg gaacaaggca taggattgga agacggtatg tacgtcgcgc ttgttggttg
    11641 gaatatcatt gatacgaaca ccgtctagca tgatatctcc tgtcgtcgca tccagtaaac
    11701 ctgcaataat gtttaggata gttgatttcc ccgaaccaga tgcacctaga agggtgtaga
    11761 atttcccttc ttccaactca aagttgatgt ctttgagaac cttggtgttg ctgtcttcaa
    11821 aaactttaga gacgtttttg aattcgataa ttggtttttt caattggcat aaattccttc
    11881 tttttcatag attaaccgat cggggctctg tcaggtcccc actacctctt gcagggagta
    11941 aaaccacctg catacatctt cgctaccgat aggctttcac cccctttcca tgacatagca
    12001 gcagcttttc aaatacagct tcctacttgc tttcacccaa gatccggact tctctttcaa
    12061 gcgtaatacc tgagtgttcc ttgacttttt cgataaccga ttggatcaag tcctcgtagt
    12121 ctttggccgt tccatctgcg acattgatca taaatcctgc atgcttttct gacacttcta
    12181 cgccaccgat acgatagcct ttcaagccag cttctgaaat taactgacct gcaaaatgcc
    12241 cgactggacg cttaaagacc gagccacaag atgggtattc caaaggttgc ttgagttcac
    12301 gtaggtgcgt caagcggtcc atttcctgct tgataacctg atgggttcct ggggctaggg
    12361 caaatttaac tgacaagaca actgcaccag actcctgaat agctgaatgg cggtaaccaa
    12421 aagccaagtc tttagcagac agggtttcga tttctccatc cttggtcaag accttacaag
    12481 actgcaagat gtgagcaatc tcgccaccat aggcacccgc attcataaag acagcaccgc
    12541 caacgcttcc tggaatacca caagcaaact caaagccagt caaactatga cggagggcaa
    12601 tgcgagttgt ttcaatcaag ttagccccag cttctgcttc aatggtatag ccatcaacag
    12661 aaacgttatt gagcttgtca cacaagatga caaatccacg aatcccacca tcacgaacga
    12721 tgatattgct tgcattgcca agaaccatcc aagggatatt ttcttggttg gcaaatttca
    12781 caaggcgcgc caactcaaaa cgatttcgtg gaaagaccaa ataatcagcc tctccaccta
    12841 cttttgtata actatagcta tgcaagggtt ccttaaaacg gatatcaatt ccttctaaga
    12901 tttcaagcat tttttctctt acagacatgt cactcttcct tttacaaaat tcattccatt
    12961 ataccatttt tagagacatt tgacgaccat aaaaatacct tgtttggatt ttgcataaga
    13021 aaaagaggtt cccccctttt tatgattttt tacaaaagat ttccttggtt ccataggcga
    13081 ccagaacgag ctccagtgct agaatcactt caaccaagac tggatttgtc aaccagccta
    13141 cttggaaaag agatggtgcc agatcaaaga aggcatgcaa gccataggct gctaggagat
    13201 aaatccattt cttctggcga acagcttggt aaacccaaac tgtcaaaagt aattggaaac
    13261 caagcgccaa gattcgctca aaaccaagca aataaatctg ccagaccgaa agtgactgaa
    13321 tggtttttaa catattttca gacagtaatt gcataacctg tggattctga gtttgaactg
    13381 ccgaaagaac gatgtaaaga ttgagtaaac taataaggcc tagaaaaatc aactccaagc
    13441 caccatgccc taggccatag gccaaggcat ctgccttttc caaacttctc tttttctcca
    13501 accatttgaa gaaaacaaga cgagccgttt cctcaaaaaa tgctgccatg gctaggccat
    13561 agataatata gataagcgga tgatcttgca agagggcaat actaccgtct ttttgagggt
    13621 gcaaaatcaa gatatgcacc agtttttcca aaatctgcga agagacaaag aaagcgactg
    13681 cacccaagcc caagacagct agattaatct ggtatttctt tttggcaaac caaatgcttc
    13741 ctatcagaaa agctagcaac agcaacatgg taataatgat atgaatggtc attttcttct
    13801 cctattctgc cttttcaata tcttttttca tctcgtcaac attgaactta gcaaacaagt
    13861 attgacggtc ttggactggg aaacgttggt ccaactggtc aactgctccc acctcgataa
    13921 tgccttcctt gatgacaaaa tccatgacat gtccaccgaa ggtcaaatca tctgatatga
    13981 agtgcagatg gtagcctgcc acactgaccc catggaaaat ctcaggcgtc cagaaaccaa
    14041 caatagtccc cgcaacattg tcacgactat attccggttg atgggttgcg acatcagcaa
    14101 acttagtatc gggtgttgac ttaggaatca tacgcacatg catatgtgaa aattctcccc
    14161 gaatcttgat agaacggaaa agattttccc catcataata agactcaatt cgttctttca
    14221 attccttgtc tgtcatctca aagcgctggc ggaaaatgac ctctgcctga tgcggtacca
    14281 ctgcagcgta aggaataagg gcatctggtg acacttccac aatttctggc tggtctcctg
    14341 accctttggc ctgataagcc ttgccatcca agacaatcaa ttccccatca atagaatcca
    14401 aggttcccaa accaaggtca ccatgctcta gcaattctcc cactgtcatg gtaccaccat
    14461 aaaggccagc catcaaggct ccaagagtat tgtattgaaa taatttcact ggttcctgca
    14521 cgttcttatc cattcacttt cttgtctgtt attctataaa tcttactcct aagtatacca
    14581 catttgcccc tagatgtgaa cgagagaaac gctctagaca ttgccaagaa ggaaaaaaaa
    14641 gggtacaatg taacaaaatc aagggaggtc tggaatgaag aaacaaagca agtacaaaga
    14701 ggtcgtttcc tatctgaaaa atggtatcga gtctggacga tttccgacgg gtagtcgcct
    14761 accttctatc cgtcaactga gccttgactt tcactgtagc aaggacacca ttcaacgagc
    14821 cctgctggaa ttacggcacg aacaatacct ctatgccaag ccccagagtg gctactatgt
    14881 cctagaacaa gggcaacacc aggacctaga aatcgaggtt accgacgaac atgccagtgc
    14941 ctatgacgat ttccgactct gtgtcaatga aaccttgatt ggccgagaaa actacctctt
    15001 caactactat gataatcaag aaggattaga agacctaaga caatccattc acaaactcct
    15061 ctttgagcaa gctctctact gcaaggctaa ccaactagta ctgacttctg gaacccaaca
    15121 agccttgttt atcctctctc aaatatcctt tcctagccaa gccaaggaaa tcttggtgga
    15181 acagccaacc taccatcgga tgaatcgcct cttgattgca caggggctgg actatcaaac
    15241 gattgaacga ggcattgatg ggattgacct gaaggagctg gagcgacact tcaagacagg
    15301 aaaaattaag tttttctaca ccattccccg atttcactat cctctgggac attcctattc
    15361 tgagcaagac aaacgatcca ttcttaactt agctgccaag tatgatgtct atatcgtaga
    15421 agacgattat ctgggtgatt tggactccaa gaagggccaa accttccact atcttgatac
    15481 agaggagcat gtcatttata tcaagtcctt ctcgaccagc ctttttcctg cccttcgtat
    15541 tacagcactc attcttccaa atgctatcaa agaagcattt gtggcctaca aaaatatcct
    15601 agactacgac agcaacctca ttatgcaaaa ggccctgtca ctctatattg acagtcaatt
    15661 gtttgaaaaa aatcgtttgg ctcgcttgac caatcatgaa tcttaccaaa aacaaatcga
    15721 ggaaaggata actaaaacac cttgtcccct tcctcattat cccctacacg atggtttatt
    15781 gctagacctg agacagtatc ctaaaatcgc cagtctcaaa cacagtcaac cgggcttgga
    15841 cttctttgaa gaggcctatt taagtacctg tccttatcaa tttgccaagg tgtccctaga
    15901 caatctggaa aatgttttaa actatttaaa agcagaattg gaatgacatc caactctgct
    15961 ttttcttatt cttcaacttc agttagactt gctgcttttt caagatagct ttggtaaatt
    16021 ccttcatcct taagtttttg gatgaccttg tctaccactt ctttcaaatc atcactattt
    16081 tttctaagag caacggcatt ggcgtcgccg tccttcatct tcaagctgac agttgcgaca
    16141 gctaagccag cgtttttagc agcataacta agtgcaacag gctcatccat atgaacagca
    16201 tctacttttc cagcctgcaa ttcattgact gcttcaccca tattagttag ggaagttaat
    16261 tgagcttttg gcaattgttc cttgaccatt gattctggaa cagtcccttt ttgggctgca
    16321 atattagcac tttctaggct agttaaatcc ttgtattttt ccacatcagc cttacgaacc
    16381 aagaaactaa tcttgttttc atagtatggg attgaaaaat caaagacttc ttttctctcg
    16441 tcagtagcac taattcctgc aactgctagg tcagccttac cagtttgaag actggtcaaa
    16501 acattgtcaa aactcatgct tgagatttcc aacttaaccc caagttcatc agcgatagcc
    16561 tgagccatgt cgatgtctgc accgactacc tggttctttc catcaaccaa tgattgaaat
    16621 tcaaagggtg catagtcagg actggttgcc acaactaatt tccccttttg cttaattgcc
    16681 tcaacagctg actgagaacc attagaacct gactggcaag ctactaagaa gaaacttgca
    16741 agaaaattac ataaaacaaa tatccatttt tttgatttca taaataaaca acctctctgt
    16801 taattttgta taattataac gatatccaag ttacttgtca agtgtttttt aaatttttat
    16861 ctcaaaaata ttttttcgtt caaaaaaagg agccatcagt tgatttcaag ctccttttta
    16921 tacagaatta aactatttta tagttcgaca atcttacctg tttcaaagta gacaacccat
    16981 tcacagatat ttttagcata gtcaccgata cgctccaagt aggaaataac ttggaaataa
    17041 tcacgacccg taacaatggc ttctggattt ttcttaatct cttcagtcgc aaggtcacgg
    17101 atagtttcaa aatagtggtt aatttgctca tccatggagg ccacccggta tgcgtcgtca
    17161 acagaaccat taagataaag atcaagtgct gcttcaacaa cgcttttaac ttcacgtccc
    17221 atttttttaa tttcttcctc tacagctgga atacgttctt cccccttcat acggatggtt
    17281 gcctgggcaa tggctacagc gtgatccccc atacgctcca catctgatac agccttaagg
    17341 acagtcaaga ctgtacgcaa atcttgagag actggttgtt ggagtgcgat catttcaaat
    17401 gatttctttt ccagtttcac ttcgtattca tttacttctg catcatcttc gatgacctct
    17461 tttgccaggt cacggtcatg cgtgacaaaa gcacgtaccg tacgattgat ttgtgagagc
    17521 acttcttgtc ccatagcgta gaactggtta tgtaatttct ctaaatcttc ttcaaattga
    17581 gatcgtaaca tctttcatct ccttatccaa attttcctgt aatatagtct tccgtttcct
    17641 tgtgttgggg atcaaggaac atctgcttgg tatcattaaa ttcaatcaaa tctccatcta
    17701 ggaaaaatcc tgtcttatca gagatacgtg aagcttgctg catggaacgg gttaccagaa
    17761 gcatggtgta cttgtctttt agaccataca aggtttcctc aattttacca gctgaaatcg
    17821 gatccaaagc cgaagttggc tcatccaaga ggatgatttt aggactagtt gccaagacac
    17881 gggccacgca gacacgctgc tgttgaccac ctgacaatcc aatagctgaa tcatatagac
    17941 gatccttgac ctcatcccag atagaggcac cttgcaaggc tttttctacg gcttcatcca
    18001 gaacctgctt atccttaatt ccattgatac gaagcccgta gacaacattc tcatagatag
    18061 tcatagggaa aggattaggt tgttggaaaa ccattccgat ttccttacgt aattcaaccg
    18121 tatctgtacg cggactgtag atgttgtgac cattgtacac cacggatcca gttgtggtca
    18181 cctctggatt gagatctccc atgcggttga gagacttgag gagggttgac ttccctgatc
    18241 cagatggacc aatcaaggct gtaatttcct taggttggaa agatagggaa acactattca
    18301 aagccttctt tttattataa taaacggaca ggtctgatac ctgtaaaatc gcatctgtca
    18361 tacggtttcc tttctaacca aagtgaccag atacatagtc attggtggac tgtagcttgg
    18421 cattttggaa aatagttgca gtcttgtcat actcaatcaa atcacccaag taaaagaagc
    18481 ctgtatagtc acttgcacga gcagcctgct gcatattatg cgttacaatg atgatggtaa
    18541 agtttttctt gagctcaaac atggtctctt ctagttgcat ggtcgcaatc ggatccaagg
    18601 ctgaggctgg ctcatccatt aagaggatat ctggcttaac agagatggca cgagcgatac
    18661 agagacgttg ttgctgacca cctgataagg tcaaggctga cttgtggaga tcgtctttaa
    18721 cctgatccca gagggcagcc tgacgaaggg aggtttctac gatttcatct aggacttgct
    18781 tatccttaac tccagcacgt tcatgcgcaa aggtaatatt acggtaaatt gacttagcaa
    18841 atggattggg gcgttgaaaa accattccaa tgtgtttacg catttcataa acgttgattt
    18901 ctggacggtt gacatcaatt ccacgataga gaatctgccc agttacttta gcaatatcaa
    18961 tggtatcatt catgcgattg agactgcgta agtaggtaga tttccccgat cccgacggac
    19021 caatcaaagc tgtaatttta tttctttcaa attgcatatc aatcccctta atggattcat
    19081 ttttaccata gtaaacatgg acatccttag tagaaagggc tactttttct tcaggaaagg
    19141 taaggatatg cttctcatcc cagttatatg ttgacatggc ttctccttta ggcagcggtt
    19201 aatttcttgt gtagatagat tccgaactta cgagctccaa agttaaaaat caggataaag
    19261 atcaggagca cagcggcaga acctgctgat acaatggttc catctggaat agtgccttca
    19321 ctattgactt tccagatatg gacagccaag gtttctgctt gacggaagat agagatgggg
    19381 ctagtcacac tgaggatatt ccagttagac cagtcaagag ctggcgccga ttgccctgct
    19441 gtatagatca gagctgcagc ttcgccaaag atacgaccag atgccaagac gacacccgtt
    19501 acaatacctg gaagcgcttc cggaataaca acatgaacca ctgtctccca gcgagaaatc
    19561 ccaagagcca gaccagcctc acgttgggta tggtgaacgt gtttcaaact atcctctaca
    19621 ttacgcgtca tctgaggcaa gttaaagact gtcaaggcca aggcacctga aatgattgaa
    19681 aacccatact caaactggac tacaaagatc aagtaaccaa agagacccac caccactgat
    19741 ggtaaagagg acaagatttc aatacaagtc cgcacaaagt tggtaacagg acctttttta
    19801 gcatattcag ccaagtaaat cccagctccc atagaaagag gtacagaaat aatcaaggta
    19861 atgaccaata ggaaaaagga attgtaaagc tgaatgccaa tcccaccacc tgcttgaaaa
    19921 gcagaagacc ttccagtcaa gaaagaccaa gagatatggg gcaagccccg aaccaagata
    19981 tagagaatta aggaagccaa gattgtcaca atgatgctag caatcgtata gaggacagct
    20041 gttgcaagtt tatctaattt cttagcgcgc ataatttttc tttcctcttt ctttcgtaat
    20101 caatttaatc acactgttaa aaactaaact catcaagagc agtaccaagg ccagtgacca
    20161 gagaacatta ttatttacag ttcccatgac agtgttccca attcccatag ttaatataga
    20221 agttaaagtt gcagctggtg tggtcaagga agttgggata acagctgagt ttccgacaac
    20281 catctggata gctaaagcct caccaaaggc acgcgccatc ccaaagacca ctgcagtgaa
    20341 aataccagaa cgggccgcct tcaagatcac acgccagata gtctgccagc gagtggctcc
    20401 catagcgaaa ctggcttcac gataataacg aggaaccgca cgcaagctat ccgttgtcat
    20461 aaaggttacg gtcggcaaaa tcatgacaaa gaggacggaa atccctgaca aaatcccaaa
    20521 accagtccca ccaaagacac tgcgaacaaa gggaacgacg acttgcaagc caataaatcc
    20581 gtacactact gaaggaatcc caaccaggag ttcaatagct ggttgcaaaa tcttcgcccc
    20641 ttttggtgat acttcggtca taaaaactgc tgcaccaata gcaaaggggg ttgcgataag
    20701 ggctgagaga atggtaacga taaaggaacc caaaatcata ggaagggcac caaattcttt
    20761 actagaagga ttccaaattc ctcccaaaag aaagtcaaag atattcacac cattgacaaa
    20821 gaaggtcgac aagccttttt gcgctacgaa aaccaaaatc atggccacaa ggatgactat
    20881 caaagaaaga caggcaaagg tcaaaccttt tcctaatttc tccagacgag aattctttga
    20941 tggaagcaac attttcttag ctaattcttc ttgattcatt attgtctccc ttccaacact
    21001 gtcacagttc cggcagcatc tttttcaacc ttcatttcct taatcggaat atacttcaat
    21061 cctttgacaa tcccttcttg ggtctcatcc gagagaacaa aattgagaaa ttctgcagcc
    21121 aactcattgg gctgccccaa tgtatacata tgctcataag accacaaggg ccaattattg
    21181 ctacttatat tttctggact taagtcatag ccattcaact tcatgctttt gaccgaatca
    21241 tctatatagg taagagataa ataagagata gctcctggac tttttgatac gattgatttt
    21301 accgctccat ttgaatcctg ctcctgactt tgcatggcag actgaccttc cataatgaca
    21361 gtatcaaagg tagcacgaga accagagccg gctgcccgat tgataacaga gatgggtaag
    21421 tccttaccac caacctcttt ccaattggtt acctcaccta tgaagatttg acgaagttgc
    21481 tctgtcgtta ggttatcaac atcaacctcc ttattgacaa tcagagccaa gccagctacc
    21541 gcgaccttgt ggtcaacaag agcagaagca tcaattccgt ctttttcctc agcaaataca
    21601 tctgagtttc ctatatcaac tgccccagac tgaacctggg acaagcctgt accagaacct
    21661 cccccttgga cattgaccgt ttttccaaca tggatcgtgc caaattcatc tgccgctact
    21721 tcaaccaagg gttgcaaggc agttgagcca acagccgtta tggattctcc acgatcaatc
    21781 cagctagcac agcctactaa acaagccgtc agccaaaaag cgataagaga cagagcaagc
    21841 ttttttcttt ttttcactgt ttttctcctc gaaaataatt atgaatactg tgaatttttt
    21901 aagtagttct ttatgagttg acgcatgaat tcttaccaaa tttctgcgca attgattatt
    21961 tatataatat aggctatatt actctttcct aacctccttt tttcatatgt ggataaaatc
    22021 tcttgtctat cccttccccc attgtcaccc attatagtca tttcgtgtct ctttttcccc
    22081 tttttaatgc aagggaaatt actctcctta gatgataatc caaaagctag aaaggtatct
    22141 caaacctctc tactctccca gactagttta caactaaaag gaaaagattc tattttatga
    22201 gaaatctagt ttacaagcgg taagaacgct aataactaaa cttcttgtac tctttgaaaa
    22261 tctcttcaaa ccagtgtttt gagctatcta tggctagctt cctagtttgc tctttgattt
    22321 tcatcgagta gtaaaactac atgtaatggc aatcaagata tcaagaatca tcctactaaa
    22381 aaaatccata ctttcactat aacatagaat aagatatttg actagcattt tcatttgaat
    22441 ctgaggcctt ttggaaaata atttttcaaa acatttccag taacctttgc aaagcccaag
    22501 ccattgcctt taaccaaaac ttggtaccaa ccatttggca gactttctgc cagctgaacg
    22561 atttctccag ccgcatactt gacaaacgct tcttggccaa tttcaaccga ctgttcgacc
    22621 tgactcgatt tcaaggctaa accaagagcg aaactgggct caaagcgttt cttcttaaaa
    22681 gtacccagat gcagtccatt gcgagcaatc ttgagcttcc ctaaatctgg caaaagttct
    22741 ggcaagagat aaagctggtc tccaaaagtc tgcaagatac ccggtagatt gaccttcaaa
    22801 tggttttggg caaattcctg ccacaaggca acttgttcac ggctgaggtt actcttactt
    22861 gccttaaatt taggagctgg attgttaccc ttaaactgta gatgggcaac aaactgaccc
    22921 tctcccttaa actgatgagg gtacatccga gccgtttctg gcaggtcaat accagctacc
    22981 attccattga tatgctctac tggcaacaag tcaaaatcat actcttccag caaccaattg
    23041 acaatctctt cgttttcctc gggtgcccag gtacaggtcg aataaaccag acgaccacct
    23101 tcagctaaca tggtcactgc atcctccaga atttctcttt gcaagctagc acattgactc
    23161 ggataatcta agctccaata gtccatagca tcaggttgct tacgaaacat tccttcacca
    23221 gagcaagggg catcaagaac gattaagtca aaatagcctt taaagacctt gaccaagcgg
    23281 tcggcagatt cattggtcac cacgacattt gtcgctccaa aacgctccat gttttcaacc
    23341 aaaatcttag cccgtttgct tgaaatttca ttggaaacaa gtagcccctc ccctgctaga
    23401 taggctgcca gttgagttga tttgcccccc ggtgcagcag ccaagtccaa gaccttcata
    23461 ccaggactgg gttgggctac ttgagccacc atttgagcag caggttcttg cgaataaact
    23521 aaacctgtag catgctcagg cgatttccct gaaaccttcc catagtggcc ccaaggggtt
    23581 tgagtaatgg catcagaaaa ggaaagttgc tcttctttta agggattgac ccgaaaggcc
    23641 gaaaccgctt cctcctcaaa agaggcaaga aaatttcttg cctcatctcc tagtatttct
    23701 ttatattttt caacaaatcc ttctggaaat tgcatttaag ttcttttcct ttcgtaaata
    23761 taggactgaa tttcctcctg catctcaaga ggcaccatca tgacaggctg tctggtttga
    23821 aaatcaggag cttcaccaaa aagggtcaca acccgatagc ccagactttc ccctaaaata
    23881 ctagctgcgg cataatccca tggttgcaga taagtgagat aggtcaacaa acgccctgac
    23941 aaaatcttgg caaaactaat ggccgcactt ccatagacac gaacaccaag aaccgctcgg
    24001 ctcaaatcag ccagccccca ttcattggtt tccagcatac cactattccc tgcaatgaga
    24061 aaatctccaa gtggtttagt tttaaaagga gctaggggcc tatcatttag acaaactgga
    24121 aattccccac caccgtggta acaatcccct ttgaccacat cataaatcag accaaactgt
    24181 ccctgaccat tttcaaaata agccatcata acagcaaaat cttcctgctg ggctacaaaa
    24241 ttattggtac catcaatggg atcaatgacc caaaccttgc cctcttgaac cgaggctcgc
    24301 agacaacctt cttcagcaca aatcttatcc tcaggataac gggacaaaat ctcaccaacc
    24361 aagagttcct gaacttcttt gtccagtcta gtcaccaaat ctgttggaga ggacttggtt
    24421 tcaacacgca agtcttcctg catatggtca agaatgtact gacctgcttt cttaacaagc
    24481 tctttagcaa attcaaattt actttccaag agaaatcttt ccttcccctt tttctttggc
    24541 agcctgaact gctcggtaaa gtgaatagcc actaaccgtt tcaaattctc gtcccaaacg
    24601 tttctcttcg ctcttgcttg gcacgaccgc tttgaatccc ttataggagt ccagtaactt
    24661 tttagcctct actttgcctt cataggcagc ttcaacatca ttaaaaaaag aaagcactga
    24721 agcaagttct tcagtgctcc acgacaaatc tagtgggtaa ctatactgtt tgttcattaa
    24781 ctaataccag ctctcattct tgcttctttt agttcttgct tacgataact acgagggaga
    24841 aaagcacgaa tctcatcttc attaaaaccg atttgcatac gcttggcatc aataataatt
    24901 ggacgacgca aaagactagg atactgctca atcaaatgaa gcaattccga taccgaaata
    24961 ctctctacat caatattcaa tttttgaaaa atttttgaac gagttgaaat gatgtcatca
    25021 gtaccatttt cggtcaagga aaggatgtgt tgcaattctt ttcttgttaa aggactggtc
    25081 ataatattgt gttccacaaa gggaacctta tgtttttcta accaggcctt agccttacga
    25141 catgatgtac agctcggtga tagaaatagt gtaatcatgc ttttctcttc ttatctatac
    25201 tttgctactt ctattataca aaaaaataaa gcgcttgact agggattttt agaaaaaaag
    25261 cctatttttt caagaaaaat aggctttttg cgaacgattg acacaattgg atttggttaa
    25321 ttc
//
LOCUS       DAVLWA010000022        24890 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLWA010000022 DAVLWA010000000
VERSION     DAVLWA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.022"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            49..483
                     /locus_tag="VYN80_000878"
     CDS             49..483
                     /locus_tag="VYN80_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1540816.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            495..1685
                     /locus_tag="VYN80_000879"
     CDS             495..1685
                     /locus_tag="VYN80_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1540817.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1696..2187
                     /locus_tag="VYN80_000880"
     CDS             1696..2187
                     /locus_tag="VYN80_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1540818.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2202..2981
                     /locus_tag="VYN80_000881"
     CDS             2202..2981
                     /locus_tag="VYN80_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1540819.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2968..3786
                     /locus_tag="VYN80_000882"
     CDS             2968..3786
                     /locus_tag="VYN80_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1540820.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3786..4079
                     /locus_tag="VYN80_000883"
     CDS             3786..4079
                     /locus_tag="VYN80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1540821.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4101..6002
                     /locus_tag="VYN80_000884"
     CDS             4101..6002
                     /locus_tag="VYN80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1540822.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKRSLERLMNNRF
                     MFDSPWDMEPCSKIYQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKSYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPILAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6062..7063
                     /locus_tag="VYN80_000885"
     CDS             6062..7063
                     /locus_tag="VYN80_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1540823.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7173..7508)
                     /locus_tag="VYN80_000886"
     CDS             complement(7173..7508)
                     /locus_tag="VYN80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540824.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7519..7761)
                     /locus_tag="VYN80_000887"
     CDS             complement(7519..7761)
                     /locus_tag="VYN80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540825.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTMLGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7777..7971)
                     /locus_tag="VYN80_000888"
     CDS             complement(7777..7971)
                     /locus_tag="VYN80_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540826.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            8137..9087
                     /gene="rsmH"
                     /locus_tag="VYN80_000889"
     CDS             8137..9087
                     /gene="rsmH"
                     /locus_tag="VYN80_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1540827.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            9099..9416
                     /gene="ftsL"
                     /locus_tag="VYN80_000890"
     CDS             9099..9416
                     /gene="ftsL"
                     /locus_tag="VYN80_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1540828.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9420..11672
                     /gene="pbpX"
                     /locus_tag="VYN80_000891"
     CDS             9420..11672
                     /gene="pbpX"
                     /locus_tag="VYN80_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1540829.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGINFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            11674..12654
                     /locus_tag="VYN80_000892"
     CDS             11674..12654
                     /locus_tag="VYN80_000892"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1540830.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLFAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13263..15368
                     /locus_tag="VYN80_000893"
     CDS             13263..15368
                     /locus_tag="VYN80_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1540831.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(15666..16148)
                     /locus_tag="VYN80_000894"
     CDS             complement(15666..16148)
                     /locus_tag="VYN80_000894"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1540832.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(16243..17727)
                     /locus_tag="VYN80_000895"
     CDS             complement(16243..17727)
                     /locus_tag="VYN80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1540833.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDESAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGKIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            17880..19490
                     /locus_tag="VYN80_000896"
     CDS             17880..19490
                     /locus_tag="VYN80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1540834.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLNYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVEMTD"
     gene            complement(19621..21051)
                     /locus_tag="VYN80_000897"
                     /pseudo
     CDS             complement(19621..21051)
                     /locus_tag="VYN80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            21207..22652
                     /gene="cps4A"
                     /locus_tag="VYN80_000898"
     CDS             21207..22652
                     /gene="cps4A"
                     /locus_tag="VYN80_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1540835.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFIKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKRILLTTTPRDSYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            22654..23385
                     /locus_tag="VYN80_000899"
     CDS             22654..23385
                     /locus_tag="VYN80_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1540836.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            23394..24086
                     /gene="cpsC"
                     /locus_tag="VYN80_000900"
     CDS             23394..24086
                     /gene="cpsC"
                     /locus_tag="VYN80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1540837.1"
                     /translation="MKEHNTLEIDILQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGTYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNILIGFLAGGVGTGVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            24096..24779
                     /gene="cpsD"
                     /locus_tag="VYN80_000901"
     CDS             24096..24779
                     /gene="cpsD"
                     /locus_tag="VYN80_000901"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV1540838.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVILNKFDVQHKKYGSY
                     GDYGNYGKK"
BASE COUNT         7452 a         4382 c         5624 g         7432 t
ORIGIN      
        1 tggggaatat aaagtaaacc ggtaaaccta aacaagaaag gagaaaagat gaaaattgta
       61 cttgtagggc atggacattt tgctacaggg atttatagtt ctttacaatt gattgcaggc
      121 aatcaagaaa atgtggaggc gattgacttt gtggaaggaa tgtcagcaga tgaactcaag
      181 caaaaaatct tacttgcaat ttcaaatgaa gaagaagttt taatcctaag tgatctcttg
      241 ggaggatcgc cattcaaggt ttcttctacc ataatgggag aaaatccagc caagacaatg
      301 aatgttctct cgggtttgaa cttagccatg ttaatggaag cagtctttgc tagaatggct
      361 catagctttg atgaggttgt taataaatca gtagtggcgg cccagggcgg agttgtaaat
      421 ggtaaagaat tgttttcaac ggatgcagag gaagaggaag aagatttcga atcgggtatt
      481 taaagggcaa aagaatgata aaaaaggtta cgattgaaaa aataaaatcg cctgagcgct
      541 tcttagaagt accacttctg acgaaagaag aagtcggcca ggcaatcgat aaggttattc
      601 ggcagttaga actcaacctt gactatttca aggaagattt cccgacgcca gctacctttg
      661 ataatgtcta tccaatcatg gataacacgg aatggaccaa tggtttctgg acaggagaac
      721 tgtggttggc ttatgaatac agtcaacagg atgcatttaa aaacatcgct cataaaaatg
      781 ttctttcttt cctggatcgt gtcaataaga gagtagaatt ggatcaccat gatctcggct
      841 tcttgtacac accgtcttgt atggctgaat ataagataaa tggagatgga gaggctagag
      901 aagcagcctt gaaagctgca gataagttga ttgaacgcta tcaagaaaaa ggtggtttta
      961 ttcaagcttg gggagacttg ggcaagaaag agcattaccg tttgattatc gactgcttgc
     1021 tcaatatcca actcttattc tttgcttatc aagaaacagg cgatcaaaaa tactacgata
     1081 ttgcagaaag tcatttctat gcttcagcta ataatgtaat ccgtgatgac gcttcgtcct
     1141 tccacacctt ctattttgat cctgagacag gtcaaccctt taaaggtgta acgagacaag
     1201 ggtatagtga tgattcatgc tgggcacgtg gtcaatcatg gggagtctat ggtattcctt
     1261 tgacttatcg tcacttaaaa gacgagtcct gctttgactt gtttaagggt gtgaccaatt
     1321 atttcttgaa tcgtctgcca aaagatcatg tgtcctattg ggatttgatt tttaatgatg
     1381 gtagtgatca atcacgagat tcttcagcaa cagctatcgc cgtctgtggg attcatgaaa
     1441 tgctaaaaca tcttccagag gtggatgctg acaaagatat ttataaacat gctatgcatg
     1501 ccatgcttcg ttccttgatc gaacattatg caaatgatca atttacccct ggtgggacaa
     1561 gtctcctcca cggtgtgtac tcatggcatt caggtaaagg agtggatgaa ggcaatatct
     1621 ggggtgacta ctattaccta gaagccctta tccgtttcta caaagactgg aacctatatt
     1681 ggtaggagga gaaatatgac aatgccaaat attattatga cccgtatcga tgaacggttg
     1741 attcatggac aaggacaact ttgggtaaaa tacctaggtt gtaatacggt cattgttgcc
     1801 aatgacgaag taagcacgga caagatgcaa caaactctga tgaaaacagt tgtgccagac
     1861 tcagttgcca tgcgtttctt ccctttgcaa aaggtgattg atatcattca caaggctaat
     1921 cctgctcaaa cgatctttat cgttgtaaag gatgtgaagg acgctttaac cttggtagaa
     1981 ggtggtgtcc ctatcaaaga aatcaatatt gggaacattc acaatgctac tggtaaagag
     2041 caagtgacac gctccatctt cctgggtgaa gaggacaagg cggccctcaa ggaattgagc
     2101 caaactcatc aagtaacatt taatacgaaa acaactccaa caggaaatga tggagctgtt
     2161 caagtcaaca ttatggacta tatttaaaag aggagatcgt tatgtcgatt aatgtatttc
     2221 aagcgatttt aattggatta tggacagctt tctgttttag tggaatgctg ttaggaattt
     2281 acaccaatag atgtattgtt ctgtcatttg gtgtcggaat tattctaggt gatctgccta
     2341 ctgctcttgc aatgggagct attggtgaat tggcttatat gggattcggt gttggtgctg
     2401 gaggtactgt tccaccaaac ccaatcggac ctggtatctt tggtaccttg atggctatca
     2461 ctagtgctgg taaagtcagt ccagaagcgg ctcttgccct ctctactccg attgctgtgg
     2521 cgattcaatt cttacaaact ttcgcctaca ctgtacgtgc tggtgcgcct gaaacagcga
     2581 tgaagcactt gaaaaaccat aatttgaaga aatttaagtt cactctaaat gcaacaattt
     2641 ggttgtttgc ctttattgga tttaccttgg gttgcttggg tgccctttca atggatacct
     2701 tgttgaaact cgtagactac attccaccgg tattgcttac aggtttgaca gttgctggta
     2761 aaatgctccc agctatcggt tttgcgatga tcttgtcagt gatggctaag aaagagttga
     2821 ttccctttgt cttgttggga tatgtttgtg cagcttatct aaacatccca acaattggta
     2881 ttgcaattgt aggtactatc tttgctttga ttgaatttta taacaagcca aaaacagcgg
     2941 atcatgtggt agaggaggaa gcacacgatg actggatcta acaaattaac aaaacgtgat
     3001 tatcttaaaa cgtctttgcg ggcattcttt tgtcaaaatg gatttaacta tagtaactat
     3061 caagggttgg gatatgccaa tgtgatgtat cctgctttga aaaaacacta tggagaggat
     3121 caggaaggtt tctaccaagc cttggaagaa aactgtgaat tctataatac caacccacac
     3181 ttcctgcctt ttattaccag cttgcatctt gtaatgttgg aaaatggccg tccggcaaaa
     3241 gaaacacgta gcatcaagat ggccttgatg ggaccattgg caggtattgg ggattctctt
     3301 tctcaattct gtttagctcc attgttctca accatcgcag cttcgtttgc tcaagaaggc
     3361 ttggttgtcg gtccaatctt gttcttcctt gcgatgaata cgattttaac agcgattaaa
     3421 ttgtcaactg gtctgtatgg atacaaacta ggaacaactg tgattgataa actaagcgaa
     3481 cagatggcaa cgatttctcg tattgccaat attatcggtg taaccgtaat tgctggtttg
     3541 gcagcgacat ctgttaagat tatggtgccg attacctttg ctgcagggga agttaaagca
     3601 gacgctaaac aaagtatcgt aagtattcag ggaatgcttg ataaggttgc tccagctctt
     3661 ctaccagccc tatttacact tttagtttac tacttgatca aagaaaagaa atggacaaca
     3721 tataaactcg ttattttaac agttatcatc ggaattatcg gaagctggct taagattata
     3781 gcttaatgga tacaacattg ttttatggaa tagtgattgt cttggcagtg agtccccttt
     3841 tactgtcaag ctttcattct attcgtcagc aaaagttgct tcgcaaacag atggagcaac
     3901 gacaagagta tttagcttct ttaacatctg gtgatgaagt gttgttattg tctggaattc
     3961 atggaaaaat catttctatc caagatgact tggtctcctt gcagattgca aaaggtgtgg
     4021 tcatctatgt agaaaaggaa agtgtaatgg gaaagacaaa agaactgctt tttaagtagt
     4081 tctttttctg gtaatagata atgaaagaag aaagaagaca attttttgaa agagtcgatg
     4141 gaaaccaatg tcgtgattat atcttgtcgc actgttcaaa agactatgag aaagtcaagc
     4201 gttcccttga acgcttgatg aacaatcgtt ttatgtttga tagtccttgg gatatggagc
     4261 cttgttcaaa aatctatcaa atccagccga tggtatggga tcaagtattt gaagatgatc
     4321 cagaatgggc ttatatgctc aatcgacaag aatatctctt gcagtttatg atagggtatg
     4381 tggtagaagg agataagagc tatattcaaa agtgcaagtt ctttctattt gattggattg
     4441 agcaggtgag agaattttct cctcaatctt tgatgactag aaccttggat acgggtattc
     4501 gttcctttac ttggttgaaa ctactcttgc tcctcttgaa atttgacttg ctagaggaga
     4561 aagaactaga gaaaattttg gtcagtctag aaaagcagat tgactttatg aaaagctact
     4621 atcgcgccaa gtacaccctt agtaactggg ggattttaca aacaattccg atacttgcta
     4681 tctatcattt cttttcagat aagatggacc tagaagaagc ttatcatttt gcttcagagg
     4741 agttgaaaca gcaaattgag acacagattt taggagatgg aagccagttt gaacagtcga
     4801 ttctctatca tgtagaggtt tataaagcct tgctggattt gtgtctcttg cttccagact
     4861 tgcaagatag ttaccaagag ttgctggaaa agatggcgac ctatattcaa atgatgacag
     4921 gcttagatgg acggactttg gcttttggtg atagcgattc tacagaaacg acagaaatgt
     4981 tgagcctgtc tgctgtggtt ttgaacaagg aagaccttct taacggtctg gatgttaaag
     5041 ttgatttgct tagcctcttg ttcctggggc gagaaaaggt caagcgactg caggaatttg
     5101 aaaggagagc ttggcagcct aagtccatga tctttgaaga ctctggacat gtctgtatta
     5161 aggatgaaca tcgttatcta tttttcaaaa atggtccgct aggaagcgcc catagccata
     5221 gtgacgagaa tagtttttgc ttacagtatc aaggccaacc tattttcata gatgctgggc
     5281 gttattctta tcgggagata tatgaacgtt atctcttaaa gagtgcttgg agtcattcga
     5341 cctgcattgt agatgggaaa gctccggaaa gaatcacggg atcctgggaa tatgaatact
     5401 atcctcactc cctgttttgt caccataaag aaagggaggg agtgcattat attgaagggg
     5461 cttattggtc agcagaacct gatttgcctt atcttcacag gagaaaaatc ctcatgttgg
     5521 tagaggatgt ctggctcttg gtagatgaca tcaggtgtca aggtcaacat gaggtgttga
     5581 ctcagtttat ccttgacaag gatgtgacct atcaagatgg gaaaatcaat cagttgagac
     5641 tatggagtga agttgatttt gatttggaag ataccatcat ttctcctaaa tacaatgagc
     5701 ttgaaagaag tagcaaactc accaagcgcc aattctttga gaatcagatg ctggattata
     5761 ccatcattgc gcatgagagt tttgaaatca tccgtcattc tgtctaccag acagatgatc
     5821 gtgaagtgga aaatgctctg gcttttgaag tgaaaaatga cgaaacagac aagctgattc
     5881 tgttattaag cgaggatatt tgtgtaggtg aaaaattgtg cctcgttgac ggaacaaaaa
     5941 tgcgtggaaa atgtctagta tatgataaaa taaatgagag aatgattcgc ttgcagtgct
     6001 agaaataggc attttgaata gtgaatatgt tataatgagt attagtagga ggtgttttag
     6061 attggagaag aaactgacca taaaagacat tgcggaaatg gctcagacct cgaaaacaac
     6121 cgtgtcattt tacctaaatg ggaaatatga aaaaatgtcc caagagacac gtgaaaagat
     6181 tgaaaaagtt attcatgaaa caaattacaa accgagcatt gttgcgcgta gcttaaactc
     6241 caaacgaaca aaattaatcg gtgttttgat cggtgatatt accaacagtt tctcaaacca
     6301 aattgttaag ggaattgagg atatcgccag ccagaatggc taccaggtaa tgataggaaa
     6361 tagtaattac agccaagaga gtgaggaccg gtatattgaa agcatgcttc tcttgggagt
     6421 agacggcttt attattcagc cgacctctaa tttccgaaaa tattctcgta tcatcgatga
     6481 gaaaaagaag aaaatggtct tttttgatag tcagctctat gaacaccgga ctagctgggt
     6541 taaaaccaat aactatgatg ccgtttatga catgacccag tcctgtatcg aaaaaggtta
     6601 tgagcatttt ctcttgatta cagcggatac gagtcgtttg agtactcgga ttgagcgggc
     6661 aagtggtttt gtggatgctt taacagatgc taatatgcgt cacgccagtc taaccattga
     6721 agataagcat acgaatttgg aacaaattaa ggaattttta caaaaagaaa tcgatcccga
     6781 tgaaaaaact ctggtattta tccctaactg ttgggcccta cctctagtct ttaccgttat
     6841 caaagagttg aattataact tgccacaagt tgggttgatt ggttttgaca atacggagtg
     6901 gacttgcttt tcttctccaa gtgtttcgac gctggttcag ccctcctttg aggaaggaca
     6961 acaggctaca aagattttga ttgaccagat tgaaggccgc aatcaagaag aaaggcaaca
     7021 agtcttggat tgtagtgtga attggaaaga gtcgactttc taaaatgaag gaaaatgact
     7081 tgcaatctct gttaagaaat aaaataatcc cacctagaac aagctaggtg ggattatttg
     7141 cctatgaaat gagaaattat gggagcaagc tcctaaatca actgtttttg atctacttct
     7201 ttaactactt gataaaagtt atagaagtag gccaaacttg aaatgatggt tacgactagg
     7261 aatattgaaa atttccattg gacagggttg gttaaaagtt gtggaaagga tatgaggaga
     7321 aagaagaggg ctgcgttgag gacaggtatc cgttttgatt gtattttctc aagtccttta
     7381 ttgagcgcag gaagaaagag gagtaggagt agtaaaactg tatgagaaac agctcctgaa
     7441 ataagggcga agaaaaggaa aatactgata aaaacatgaa tgatcagtag tctagctagt
     7501 gatttcataa ggcacctcct aatcctggtc ttttttagct cttgcaatac gaagtgagtc
     7561 gacaatatgt atcatcactc cgaaaaagaa agctcccagc atagttttaa aaatatgttt
     7621 tgtatttaga agagaactga taaaatttgg attttcactt gttagggtat caatgagtgg
     7681 aattataaaa aatatcactg ttccataaat cgaacctgct ttcagaccag gataacgtaa
     7741 ctgtctcttt tcttttttca tgagtttgct ccttttttaa aaatcttcct tccaaaagag
     7801 actgttgagg tcagtttgga ggctgcgagc gagattgaga cagagttcca gagttggatt
     7861 gtacttgtcg ttttcaatca tattgatggt ctgtctcgag acaccgatat ccttggcgag
     7921 ctcgagctgg gaaatgccca gttccttgcg aaattctttc acacgattca tctggtctcc
     7981 tttctgattt gtgtcgtata tatttgactt tattatagtc ttttaaacat aaagtgtcaa
     8041 gtatttttga catatttttt gaagaaatag tagtctcctt gtcctatttg tctgacaagt
     8101 gcaagctggt cggatttgtg gtaaaataga taagatatga caaaagaatt tcatcatgta
     8161 acggtcttac tccacgaaac gattgatatg cttgacgtaa aacctgacgg tatctacgtt
     8221 gatgcgactt tgggtggagc aggccatagc gagtatttat taagtaaatt aagtgaaaaa
     8281 ggccatctct atgcctttga ccaggatcag aatgccattg acaatgcgca aaaacgcttg
     8341 gcaccttaca ttgagaaggg aatggtgacc tttatcaagg acaacttccg tcatttacag
     8401 gcacgtttgc gcgaagctgg tgttcaggaa attgatggaa tttgttatga cttgggagtg
     8461 tctagtcctc aattagacca gcgtgagcgt ggtttttctt ataaaaagga tgcgccactg
     8521 gacatgcgga tgaatcagga tgctagtctg acagcctatg aagtggtgaa caattatgac
     8581 tatcatgact tggttcgtat tttcttcaag tatggagagg acaaattctc taaacagatt
     8641 gcgcgtaaga ttgagcaagc gcgtgaagtg aagccgattg agacaacgac tgagttagca
     8701 gagattatca agttggtcaa acctgccaag gaactcaaga agaaggggca tcctgctaag
     8761 cagattttcc aggctattcg aattgaagtc aatgatgaac tgggggcggc agatgagtcc
     8821 atccagcagg ctatggatat gttggctctg gatggtagaa tttcagtgat tacctttcat
     8881 tccttagaag accgcttgac caagcaattg ttcaaggaag cttcaacagt tgaagttcca
     8941 aaaggcttgc ctttcatccc agatgatctc aagcccaaga tggaattggt gtcccgtaag
     9001 ccaatcttgc caagtgcgga agagttagaa gccaataacc gctcgcactc agccaagttg
     9061 cgcgtggcca gaaaaattca caagtaagag ggaaaaagat ggcagaaaaa atggaaaaaa
     9121 caggtcaaat actacagatg caacttaaac ggttttcgcg tgtggaaaaa gctttttact
     9181 tttccattgc tgtaaccact cttattgtag ccattagtat tatttttatg cagaccaagc
     9241 tcttgcaagt gcagaatgat ttgacaaaaa tcaatgcgca gatagaggaa aagaagaccg
     9301 aattggacga tgccaagcaa gaggtcaatg aactattacg tgcagaacgt ttgaaagaaa
     9361 ttgccaattc acacgatttg caattaaaca atgaaaatat tagaatagcg gagtaagata
     9421 tgaagtggac aaaaagagta atccgttatg cgaccaaaaa tcggaaatcg ccggctgaaa
     9481 acagacgcag agttggaaaa agtctgagtt tattatctgt ctttgttttt gccatttttt
     9541 tagtcaattt tgcggtcatt attgggacag gcactcgctt tggaacagat ttagcgaagg
     9601 aagctaagaa ggttcatcaa accacccgta cagttcctgc caaacgtggg actatttatg
     9661 accgaaatgg agtcccgatt gctgaggatg caacctccta taatgtctat gcggtcattg
     9721 atgagaacta taagtcagca acgggtaaga ttctttacgt agaaaaaaca caatttaaca
     9781 aggttgcaga ggtctttcat aagtatctgg acatggaaga atcctatgta agagagcaac
     9841 tctcgcaacc taatctcaag caagtttcct ttggagcaaa gggaaatggg attacctatg
     9901 ccaatatgat gtctatcaaa aaagaattgg aagctgcaga ggtcaagggg attaatttta
     9961 caaccagtcc caatcgtagt tacccaaacg gacaatttgc ttctagtttt atcggcctag
    10021 ctcagctcca tgaaaatgaa gatggaagca agagcttgct gggaacctct ggaatggaga
    10081 gttccttgaa cagtattctt gcagggacag acggcattat tacctatgaa aaggatcgtc
    10141 tgggtaatat tgtacccgga acagaacaag tttcccaacg aacgatggat ggtaaggatg
    10201 tttatacaac catttccagc cccctccagt cctttatgga aacccagatg gatgcttttc
    10261 aagagaaggt aaaaggaaag tacatgacag cgactttggt cagtgctaaa acaggggaaa
    10321 ttctggcaac aacgcaacga ccgacctttg atgcagatac aaaagaaggc attacagagg
    10381 actttgtttg gcgtgatatc ctttaccaaa gtaactatga gccaggttcc actatgaaag
    10441 tgatgatgtt ggctgctgct attgataata atacctttcc aggaggagaa gtctttaata
    10501 gtagtgagtt aaaaattgca gatgccacga ttcgagattg ggacgttaat gaaggattga
    10561 ctggtggcag aatgatgact ttttctcaag gttttgcaca ctcaagtaac gttgggatga
    10621 ccctccttga gcaaaagatg ggagatgcta cctggcttga ttatcttaat cgttttaaat
    10681 ttggtgttcc gacccgtttc ggtttgacgg atgagtatgc tggtcagctt cctgcggata
    10741 atattgtcaa cattgcgcaa agctcatttg gacaagggat ttcagtgacc cagacgcaaa
    10801 tgattcgtgc ctttacagct attgctaatg acggtgtcat gctggagcct aaatttatta
    10861 gtgccattta tgatccaaat gatcaaactg ctcggaaatc tcaaaaagaa attgtgggaa
    10921 atcctgtttc taaagatgca gctagtctaa ctcggactaa catggttttg gtagggacgg
    10981 atccggttta tggaaccatg tataaccaca gcacaggcaa gccaactgta actgttcctg
    11041 ggcaaaatgt agccctcaag tctggtacgg ctcagattgc tgacgagaaa aatggtggtt
    11101 atctagtcgg gttaaccgac tatattttct cggctgtatc gatgagtccg gctgaaaatc
    11161 ctgattttat cttgtatgtg acggtccaac aacctgaaca ttattcaggt attcagttgg
    11221 gagaatttgc caatcctatc ttggagcggg cttcagctat gaaagactct ctcaatcttc
    11281 aaacaacagc taaagctttg gagcaagtaa gtcaacaaag tccttatcct atgcctagtg
    11341 tcaaggatat ttcacctggt gatttagcag aagaattgcg tcgcaatctt gtacaaccca
    11401 tcgttgtggg aacaggaacg aagattaaaa acagttctgc tgaagaaggg aagaatcttg
    11461 ccccgaatca gcaagtcctt atcttatctg ataaagcaga ggaagttcca gatatgtatg
    11521 gttggacaaa ggagactgct gagacccttg ctaagtggct caatatagaa cttgaatttc
    11581 aaggttcggg ctctactgtg cagaagcaag atgttcgtgc taacacagct atcaaggaca
    11641 ttaaaaaaat tacattaact ttaggagact aatatgttta tttccatcag tgctggaatt
    11701 gtgacatttt tactaacttt agtaggaatt ccggccttta tccaatttta tagaaaggcg
    11761 caaattacag gccagcagat gcatgaggat gtcaaacagc atcaggcaaa agctgggact
    11821 cctacaatgg gaggtttggt tttcttgatt acttctgttt tggttgcttt ctttttcgcc
    11881 ctatttagta gccaattcag caataatgtg ggaatgattt tgttcatctt ggtcttgtat
    11941 ggcttggtcg gatttttaga tgactttctc aaggtctttc gtaaaatcaa tgaggggctt
    12001 aatcctaagc aaaaattagc tcttcagctt ctaggtggag ttatcttcta tcttttctat
    12061 gagcgcggtg gcgatatcct gtctgtcttt ggttatccag ttcatttggg atttttctat
    12121 attttcttcg ctcttttctg gctagtcggt ttttcaaacg cagtaaactt gacagacggt
    12181 gttgacggtt tagctagtat ttccgttgtg attagtttgt ttgcctatgg agttattgcc
    12241 tatgtgcaag gtcagatgga tattcttcta gtgattcttg ccatgattgg tggtttgctc
    12301 ggtttcttca tctttaacca taagcctgcc aaggtcttta tgggtgatgt gggaagtttg
    12361 gccctaggtg ggatgctggc agctatctct atggctctcc accaagaatg gactctcttg
    12421 attatcggaa ttgtgtatgt ttttgaaaca acttctgtta tgatgcaagt cagttatttc
    12481 aaactgacag gtggtaaacg tattttccgt atgacgcctg tacatcacca ttttgaactt
    12541 gggggattgt ctggtaaagg aaatccttgg agcgagtgga aggttgactt cttcttttgg
    12601 ggagttgggc ttctagcaag tctcttgacc ctcgcaattt tgtatttgat gtaagaatgg
    12661 cactctgatg tttcagggtg tttttgtgtt taaatacaca atgaaaatca aagaacaaac
    12721 tagaaagcta actttaggct gctcaaaaca tagtggtttg aaactagaat agtacacatc
    12781 tacttctaaa atattgttag aaatcgattt gactgtcctg atcgatttgt cctgttctta
    12841 tttcatttta atatacagtt ttgagatggg tgatagaact gacgaagtca gctcaaaaca
    12901 cggttttgag gttgtagata aggagaagct gatgtggttt gaagaggttt tcgaagagtg
    12961 taaaaaaata ttggtcagtt aaagtcaaaa ctcttgacct tttctgacca atgtagtata
    13021 ttatgaacgt aaggtaaatg aaagccttac tagaacactt atttaaagta aaatagaaag
    13081 aggtgggtct atgtttcatc tagaaatctt cagaagtaaa gatggtctac tcctgcttga
    13141 aaaagaaaaa ccagaaatag tacgtagggt agcggtttag ctagtctaaa ttttttaaaa
    13201 caatggtcaa agatagtcaa tatcagtaat cataactaag taaacaaaaa gaggtaaaga
    13261 atatgaataa caactttaat aattttaata acatggatga tttatttaac caattgatgg
    13321 gtggtatgcg aggatatagt tctgaaaatc gccgttactt aattaatgga cgcgaagtca
    13381 cacctgagga atttgctcac tatcgtgcga ctggtcaatt accaggaaat gcagaaactg
    13441 atgtgcaaat gccacaacag gcatcaggta tgaaacaaga cggtgtcctt gcaaaactag
    13501 gtcgaaactt gacagcagaa gcgcgtgagg gcaagttgga tcctgttatc ggacgaaaca
    13561 aggaaattca agaaacatct gaaatcctct cacgccgcac caagaacaat cctgttttgg
    13621 tcggagatgc aggtgttggt aagacagcag ttgtcgaagg tctagcgcaa gccattgtga
    13681 acggagatgt tcctgctgct atcaagaaca aggaaattat ttctattgat atctcaggtc
    13741 ttgaggctgg tactcaatac cgtggtagct ttgaagaaaa tgtccaaaac ttagtcaatg
    13801 aagtgaaaga agcagggaat attatcctct tctttgatga aattcaccaa attcttggtg
    13861 ctggtagcac tggtggagac agtggttcta aaggacttgc ggatattctc aagccagctc
    13921 tctctcgtgg agaattgaca gtgattgggg caacaactca agacgaatac cgtaacacca
    13981 tcttgaagaa tgctgctctt gctcgtcgtt tcaacgaagt gaaggtcaat gctccttcag
    14041 cagagaatac ttttaaaatt cttcaaggca ttcgtgacct ctatcaacaa caccacaatg
    14101 tcatcttgcc agacgaagtc ttgaaagcag cggtggatta ttctgttcaa tacattcctc
    14161 aacgtagctt gccagataag gctattgacc ttgtcgatgt aacggctgct cacttggcgg
    14221 ctcaacatcc agtaacagat gtgcatgctg ttgaacgaga aatcgaaacg gaaaaagaca
    14281 agcaagaaaa agcagttgaa gcagaagatt ttgaagcagc tctaaactat aaaacacgca
    14341 ttgcagaatt ggaaaagaaa atcgaaaacc acacagaaga tatgaaagtg actgcaagtg
    14401 tcaacgatgt ggctgaatcc gtggaacgaa tgacaggtat cccagtatcg caaatgggag
    14461 cttcagatat cgaacgtttg aaagatatgg ctcatcgctt gcaagacaag gtgattggtc
    14521 aagataaggc cgtagaagct gtagctcgtg ctatccgtcg taaccgtgct ggttttgatg
    14581 aaggaaatcg cccaatcggc agcttcctct ttgtaggatc tactggggtt ggtaagacgg
    14641 agcttgctaa gcaattggca ctcgatatgt ttggaaccca ggatgcgatt atccgtttgg
    14701 atatgtctga atacagtgac cgcacagctg tttctaagct aattggtaca acagcaggct
    14761 atgtgggtta tgatgacaat agcaatacct taacagaacg tgttcgtcgc aatccatact
    14821 ctatcattct cttggatgaa attgaaaagg ctgatcctca agttattacc cttctcctcc
    14881 aagttctaga tgatggtcgt ttgacagatg gtcaaggaaa tacagtaaac ttcaagaaca
    14941 ctgtcattat tgcgacctca aatgctggat ttggctatga agccaacttg acagaagatg
    15001 cggataaacc agaattgatg gaccgtttga aacctttctt ccgtccagaa ctcctcaacc
    15061 gctttaatgc agtcatcgag ttctcacact tgactaagga agacctttct aagattgtag
    15121 atttgatgtt ggctgaagtt aaccaaacct tggctaagaa agacattgac ttggtagtca
    15181 gtcaagcggc taaagattat atcacagaag aaggttacga cgaagtcatg ggggttcgtc
    15241 ctctccgtcg cgtggttgaa caagaaattc gtgataaggt gacagacttc cacttggatc
    15301 atttagatgc taaacatctg gaagcagata tggaagatgg cgttttggtt attcgtgaga
    15361 aagtctaaga cagaattttg aggataaaaa agaaggagcc agctgaaaaa aaaactggtt
    15421 ccttttgagg tacgacgggc atgtcgtata gtagaagtgt attattctag tttcaatcta
    15481 ctatagtagc tcagaagtcg gtacttaaac gtgctatatc aaaaccagtc cttgagaaac
    15541 gtggactggt ttcgtgtttg gattattacc ttgaacgaca tgcgttaaaa gttagttgaa
    15601 ccgccgtatg ccgaacggca cgtacggtgg tgtgagaagg gctagagatt atcccctact
    15661 cgattttaaa tcacatgacg ttcaaaggca tcatctgaaa tcccttgttc caagatgagt
    15721 tttgcccatt ctttagcaga gaagaggctg tggtccttgt agtttccgca agattcgatg
    15781 gttgtccctg ggacatcttc ccaagtagta gtttcagcga tttccttgag cgaatccttg
    15841 ataacagctg cgattttagc actggtgtga cgtccccaca taatcatgtg gaagcctgtg
    15901 cggcaaccaa atggtgaaca gtcaatcatg ccgtcaatgc gggtacggat gagtttggct
    15961 aagaggtgct cgatagtgtg aaggccggca gtagggatag agtcttcgtt tggttgcacc
    16021 aagcgaatat cataattgga gatgatgtct ccttttggtc ctgtttcttc cccaatcaag
    16081 cgaacatagg gtgctttgac aatggtgtgg tcaagttcaa aactttcgac aataacttct
    16141 tttgacatgg taaatccttt cagttttctt ctctcattat atcataaagg ttgttcctga
    16201 gacagagaga aaacctctcc gaggctagag aggttgaaat ctttacttac gatataagcg
    16261 gtcgtattgg tagtatgggt caaaggttac gttgataccc agtttacgaa ggacattctt
    16321 gtcttcatca gtcaagatga tggttgagtg ggcttcgctt cctttgaggt tgccgagttc
    16381 ttccatagcg cgggcagcat caggattttc tgtagctgtg atagcaagtg caatcaggat
    16441 ttcatttgaa tgaaggcgtg gattgcggct accgagatga tcgattttaa gaccttggat
    16501 tggcttaaca acttcaggct cgattagttt tacttcttta gcgatgtcag ctgatttttt
    16561 gatggcgttg atcaaggcag cggctgtagg cccaaagagt tctgagttct taccagtgac
    16621 gattttccca tttggcaatt caaaggctag ggctggtcca ccagtttctt ctgctttttg
    16681 gcgcgcaacg acagcaacct tacggtctgc aggtgtgata ccgaggtcgt tcatgagcaa
    16741 ctcaattttc ttgacggcag cttcgccaac tttttcagct ttgaagtcaa gaactgtttg
    16801 atagtaacgg cggatgattt cttgtttaga agcttcgaca gcagactcgt catctgtaat
    16861 agcgaaacca accatgttga cacccatatc tgtcggtgaa gcgtatggtg attttccgag
    16921 aatacgttcc aacatgcgct tgagcactgg gaagatttcg atatcacggt tgtagttgac
    16981 agtggtttct ccataggttt gaagatggaa ggggtcaatc atgttgacat catcaaggtc
    17041 agctgtggca gcttcataag ccaagttaac tggatgatga aggggaagat tccaaacagg
    17101 gaaggtttca aatttagcgt agccagactt gatgccattg atttggtcgt ggtacatatt
    17161 ggacatacac gttgccaatt ttccagaacc gggtccagga gcggttacga caatcaagtt
    17221 gcgactggtt ttgatgtagt cgtttttgcc catgccttct ggggaaatga tgtgatccat
    17281 atccgtcgga tatcctttga ttggataatg aagataagaa tcaattccgt ttttctcaag
    17341 ttggttgcgg aaggcatctg cagcgagttg gccagcgtat tgtgtaatga caacggaacc
    17401 aacaaaaatc cctaattcat tgaatttgtc aatcaaacga agaacttctt ggtcataaga
    17461 aatgcctaag tcgccacgtg ctttggaatg ttcaatgttg ctagcattaa tggcaatcac
    17521 aacctcaacc tgctctttca attcttgcaa gagcttgatt ttgttgtcag gttcataacc
    17581 aggaaggaca cgagcagcgt ggaaatcttc taacatttta ccgccaaact ctaagtagag
    17641 cttgccgtca aattggttaa tgcgctccaa aatatggtcg cgttgtaaat tcaaatattg
    17701 ttcagaacta aaagcttgtt ttttcatttt tttacctctg gactctatta taataaaaaa
    17761 ttggaagtta ggaaactacg gagctaaaaa agaaattaaa aagattaagc aaacgcttgc
    17821 acaaaatttt aaaaagtgct atcatatact atagattatt aaaataatga ggtaaacaga
    17881 tgcaagaaaa atggtggcat aatgccgtag tctatcaagt ctatccaaag agttttatgg
    17941 atagtaatgg agatggagtt ggtgatttgc caggtattac cagtaagttg aactatctag
    18001 ctaagctagg aatcacagcg atttggcttt ctcccgttta tgacagccct atggatgata
    18061 atggctacga tattgctgat tatcaagcga ttgcaacgat ttttggaacc atggaggata
    18121 tggatcaact gattgcggaa gctaagaagc gtgacattcg tatcatcatg gacttggtgg
    18181 ttaatcatac ctcagatgaa catgcttggt ttgtcgaagc ctgtgaaaat actgatagcc
    18241 ctgagcgaga ctactatatc tggcgcgatg aacccaatga cctagattct atctttagtg
    18301 ggtctgcttg ggaatacgat gaaaagtcag gtcaatacta tctccacttt ttcagcaaga
    18361 aacagccgga tctcaactgg gaaaatgaaa aacttcgcca gaaaatttat gagatgatga
    18421 acttctggat tgataaaggt attggtggtt tccgtatgga tgttattgac atgattggca
    18481 aaattcctga cgagaaggta gtcaataatg gtcctatgct ccatccctat ctcaaggaaa
    18541 tgaatcaggc gacctttgga gataaggatc tcttgacagt aggggagact tggggagcaa
    18601 cgccagagat tgccaagctc tactctgatc caaaggggca agaattgtct atggtcttcc
    18661 agtttgaaca tatcggtctt cagtatcagg aaggtcagcc taaatggcac tatcaaaaag
    18721 aactgaatat cgctaagtta aaagaaatct tcaacaaatg gcagacagag ttaggagttg
    18781 aggacggctg gaattccctc ttctggaaca accatgacct ccctcgtatt gtctcaatct
    18841 ggggaaatga ccaagaatac cgcgaaaaat ctgccaaagc ctttgcaatc ttacttcatc
    18901 tcatgagagg aactccttat atctaccaag gtgaggagat tgggatgacc aactatccgt
    18961 ttgaaacact agatcaagta gaagatattg aatctctcaa ctatgcgcgt gaggctcttg
    19021 aaaaaggtgt tccgatgcaa gaaatcatgg acagtatccg tgttattgga cgtgacaatg
    19081 cccgtacccc tatgcaatgg gacgagagca aaaacgctgg tttctcaaca ggtcaacctt
    19141 ggttggcagt taatccaaat tacgagatga tcaacgtcca agaagcgctg gcaaatccag
    19201 attctatttt ctatacctat cagaaactgg tccaaattcg caaggagaat agctggctaa
    19261 ttcgagctga ctttgaattg cttgatacgg ctgataaggt ctttgcttat atacgtaagg
    19321 atggtgaccg tcgcttccta gttgtggcta acttgtccaa tgaagagcaa gacttgacag
    19381 tagaaggaaa agtcaaatct gtcttgattg aaaacactgc ggctaaagaa gtacttgaaa
    19441 aacaggtctt ggctccatgg gatgctttct gtgtggaaat gactgactag aatgagcaaa
    19501 cctcaagttt ttgaagcttg aggcttttta ctatagtgga ttgaaactag aatagtgcac
    19561 ctctgcttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctattc
    19621 ttatttcatt ttgctatact tgtagttgag gagaattaag ctcctcacta gtaaactctt
    19681 cataatcctt tttattttta tgaagatatt gtttgaaaga tatgagtttc cacggatggg
    19741 tttgtggagg gatatacttg cgtctttctt tttttgttct ggttcttgtt caaagttttt
    19801 cgaatagagt tcatgattta gtagctcctt tgtgtgatag attttgtcag cgatattgag
    19861 gtagatgtct ccgtcaaatg cttttataac taatgctttc gtctttctga tgaaatagac
    19921 ttcttttcct tgttcggtag ggatatagta acgattttgg aatcggatat ggtgtccact
    19981 atcgacgact ctctccgcca gtctagccag aatgagattc ctttcagagg ggttaggaac
    20041 ctcctcgaag acagagagtt ttgtcttgtt tccaaactgt tcattaaagg tttgaatgta
    20101 ggaaagcagg aaggtattgg cttcttccaa ggtatgaata ttgttttgtt ccagttcgat
    20161 aggcaggcga gattgtagtg tctgattgag tctttcgact ctccctttag cttgagggat
    20221 agaggttgtc tcaaggagaa tccctagttg gtgacaggcg tatccaaact gtgtatgggt
    20281 atcgtcctcc attttcttag agttggaggc ttgataggta aagaccgttc tcttatctgt
    20341 tttaatttga aggggaatgc cgtgattggc taagatttgt tcgaggacat gatagtaagc
    20401 attcaagttc tcttgtttat caaaataagc gcctaggata ttgccagaag catcatcaat
    20461 ggttaagtgt aagttagatg tttgggctcc aaactaggca tgagggctgg catccatttg
    20521 aatgagttca ccagcaaatt tctttctggg tctactagga tgtacttttt tagggtcttc
    20581 aaggaagttt tcagccgtcg gtaagattgg attgtctagg gggtgattca ggttcagttt
    20641 agcttgtttt cttactctct tctttgtctt tctatgggac ttaggcgaca ggatattttt
    20701 cttatagagt atttttctaa cagttgtatc agagagctga attccttctt cttcagctag
    20761 caattcacag aaatgaagga catttggttt atatgtttca taggagaggt atttttttag
    20821 gagacgttct ttgatttcat cagggattgc atgttttggt tttcgatttc tgtttccgtg
    20881 tctgaaggct tcttttcctt tcagttgata ggctagtagc agacgattga tttgtctttc
    20941 agaaagattg agctcgacac aggctcgttt ctttgttttc tttccttggg ctatagcttt
    21001 tatcacaaga tattttttcg tttcattcat attcagttgg atccttttca tatgactatt
    21061 ctaccaaatg ggacattttc acgttcgatt tactaaagac attatcacat tcgaattaca
    21121 caagatgcag atagtgaaaa aaaggtgtag acattaccgt aaaaaagtga tataatcgta
    21181 tgatgttcaa tgtataggtg ttaatcatga gtagacgttt taaaaaatca ggttcacaga
    21241 aagtgaagcg aagtgttaat atagttttgt tgactattta tttattgtta gtttgttttt
    21301 tattgttctt aatctttaag tacaatatcc ttgcttttag atatcttaat ctagtggtaa
    21361 ctgcgttagt cctactagtt gccttggtag ggctactctt gattatctat aaaaaagctg
    21421 aaaaatttac tatttttctg ttggtgttct ctatccttgt cagctcagtg tcgctctttg
    21481 cagtacagca gtttgttgga ctgaccaatc gtttaaatgc gacttctaat tactcagaat
    21541 attcgctcag tgtcgctgtt ttagcagata gtgagatcga aaatgttacg caactgacga
    21601 gtgtgacagc accgactggg actgataatg aaaatattca aaaactacta gctgatatca
    21661 agtcaagtca gaatactgat ttgacggtcg accagagttc gtcttacttg gctgcttaca
    21721 agagtttgat tgcaggggag actaaggcca ttgtcctaaa tagtgtcttt gaaaatatca
    21781 tcgagtcaga gtatccagac tacgcatcga agataaaaaa gatttatacc aagggattca
    21841 ttaaaaaagt agaagctcct aagacgtcta agaatcagtc tttcaatatc tatgttagtg
    21901 gaattgacac ctatggtcct attagttcgg tgtcgcgatc agatgtcaat atcctgatga
    21961 ctgtcaatcg agataccaag agaatccttc ttacaacaac tcctcgagat tcatatgttc
    22021 ctattgctga tggtggaaat aatcaaaagg ataaattgac tcatgctggt atatatgggg
    22081 ttgattcatc cattcatacc ctagagaacc tttatggtgt agatattaat tattatgttc
    22141 gtttgaattt tacctcattt ttgaaattga ttgacttatt gggaggggta gatgttcata
    22201 atgatcaaga gttttcagct ctacatggga agttccattt cccagtaggg aatgtccatc
    22261 tagactctga gcaagctcta ggttttgtac gtgaacgcta ctcactagcc gatggagacc
    22321 gtgaccgtgg tcgcaaccaa caaaaggtga ttgtggctat ccttcaaaaa ttaacgtcaa
    22381 ccgaagcgct gaaaaattat agtacgatca ttgatagctt gcaagattct atccaaacaa
    22441 atatgccact tgagactatg ataaatttgg tcaatgctca gttagaaagt ggagggaatt
    22501 ataaagtaaa ttctcaagat ttaaaaggta caggtcggac ggatcttcct tcttatgcaa
    22561 tgccagacag taacctctat gtgatggaaa tagatgatag tagtttagct gtagttaaag
    22621 cagctataca ggatgtgatg gagggtagat gaaatgatag acatccattc gcatatcgtt
    22681 tttgatgtag atgacggtcc caagtcaaga gaggaaagca aggctctctt gacagaatcc
    22741 tacaggcagg gggtgcgaac cattgtctct acctctcacc gtcgcaaggg catgtttgaa
    22801 actccggaag agaagatagc agaaaacttt cttcaggttc gggaaattgc aaaagaagta
    22861 gcagatgatt tagtcattgc ttatggcgca gagatatact atactctgga tgctctagaa
    22921 aagctagaaa aaaaagaaat tcctaccctc aatgatagtc gttatgcctt gatagagttt
    22981 agtatgaaca ctccttatcg cgatattcat agcgccttga gcaagatctt gatgttggga
    23041 attactccag tcattgccca cattgagcgc tatgatgctc ttgaaaataa cgaaaaacgt
    23101 gttcgtgaac tgattgatat gggctgttac acgcaagtaa atagttcaca tgtcctcaaa
    23161 cccaaacttt ttggcgaacg ttataaattc atgaaaaaaa gagctcagta ttttttggaa
    23221 cgtgatttag ttcatgtagt tgcaagtgac atgcacaatt tagacagtag acctccatat
    23281 atgcaacagg catatgatat cattgctaag aaatatggag cgaaaaaagc gaaagaattt
    23341 tttgtagata atcccagaaa aattataatg gatcaattaa tttaggagaa aatatgaagg
    23401 aacacaacac tttggaaatc gatatattgc aactattcag agctttatgg aaaagaaagt
    23461 tggtcatttt attagtggca attataactt cttcagttgc ttttgcctac agtacttttg
    23521 ttatcaaacc tgagtttact agtacgactc ggatttatgt agttaaccgt aatcaggaag
    23581 agaagtctgg tttaaccaat caagacttgc aggcaggaac ttatctggta aaagactacc
    23641 gtgagattat cctttcgcag gatgttttgg aggaagttat ttctgatttg aaactagatt
    23701 tgacgccaaa aggtttggct aataaaatta aagtaacagt accagttgat acccgtattg
    23761 tctctgtttc agttaatgat cgagttcctg aagaggcaag ccgtatcgct aactctttga
    23821 gagaagtagc tgctcaaaaa attatcagta ttactcgtgt ttctgatgtg acaacactgg
    23881 aggaggcaag accggcgata tcaccgtctt cgccaaatat taaacgcaat atactaattg
    23941 gttttttggc agggggggtt ggaactggtg ttatagttct tcttcttgaa cttttggaca
    24001 ctcatgtgaa acgtccggaa gatatcgaag atacactgca gatgacactt ttgggagttg
    24061 taccaaactt gagtaagttg aaataggaga gaggaatgcc gacattagaa atagcacaaa
    24121 aaaaactgga gttcattaag aaggcagaag aatattacaa tgccttgtgt acaaatatac
    24181 agttgagcgg agataaacta aaagtaattt ccgttacttc tgttaaccct ggggaaggaa
    24241 aaacaactac ttccgtaaat atagcaaggt cgtttgcgcg tgcaggctat aaaactcttt
    24301 tgatcgatgg cgatactcga aattcagtta tgtcaggatt ttttaaatct cgtgaaaaaa
    24361 ttacagggct aacagaattt ttatctggga cagctgattt atctcacggt ttatgtgata
    24421 caaatattga aaatttattt gtagttcaat cggggtctgt atcaccaaac cctacagcct
    24481 tgttacaaag taaaaatttt aatgatatga ttgaaacatt gcgtaaatat tttgattata
    24541 tcattgttga tacagcacct attggaattg ttattgatgc ggcaattatc actcaaaagt
    24601 gtgatgcgtc catcttggta acagcaacag gtgaggtgaa taaacgtgat gtccaaaaag
    24661 cgaaacaaca attagaacaa acagggaaac tgtttttagg tgtcattctc aataaatttg
    24721 atgttcaaca taaaaaatat ggttcttacg gtgactatgg taattatggt aaaaaataac
    24781 ttaggaaaga ttttatggat gaaaaaggat tgaaaatttt tctggcagta ttacagagta
    24841 ttattgtcat tttattggtt tattttctta gctttgttag agagacagaa
//
LOCUS       DAVLWA010000023        24189 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLWA010000023 DAVLWA010000000
VERSION     DAVLWA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.023"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            59..223
                     /locus_tag="VYN80_000902"
     CDS             59..223
                     /locus_tag="VYN80_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1540839.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            302..1030
                     /gene="yaaA"
                     /locus_tag="VYN80_000903"
     CDS             302..1030
                     /gene="yaaA"
                     /locus_tag="VYN80_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1540840.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYTALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1081..2565)
                     /locus_tag="VYN80_000904"
     CDS             complement(1081..2565)
                     /locus_tag="VYN80_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540841.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2781..9542
                     /locus_tag="VYN80_000905"
     CDS             2781..9542
                     /locus_tag="VYN80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1540842.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGVAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKENVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGDRGERGLTGAQGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQG
                     EKGDRGERGLTGAKGEKGAQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTV
                     VRDGFDGASPLVATQRNDADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKG
                     DKGEQGLQGRDGEQGPKGEDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDG
                     ASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGK
                     DGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTL
                     TFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGD
                     TFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEK
                     GDQGERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPG
                     KDGKSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSV
                     QNGKNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPD
                     VTTTIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEG
                     NIKGPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEA
                     HQGKDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDG
                     HSTDITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENN
                     DGKYTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPE
                     KPLAVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAT
                     GQDGQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSP
                     SRIRRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPT
                     ITTKDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGS
                     TKETVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTE
                     NPDGSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTT
                     ETTVRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTI
                     KVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSV
                     SPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINS
                     DGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEV
                     PVKPVPAQPTPNVPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVP
                     EQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVG
                     RRKEA"
     gene            complement(9779..10327)
                     /locus_tag="VYN80_000906"
     CDS             complement(9779..10327)
                     /locus_tag="VYN80_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1540843.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            10648..11136
                     /locus_tag="VYN80_000907"
     CDS             10648..11136
                     /locus_tag="VYN80_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1540844.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            11133..12320
                     /locus_tag="VYN80_000908"
     CDS             11133..12320
                     /locus_tag="VYN80_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1540845.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            12313..12537
                     /locus_tag="VYN80_000909"
     CDS             12313..12537
                     /locus_tag="VYN80_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1540846.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            12694..14040
                     /gene="asnS"
                     /locus_tag="VYN80_000910"
     CDS             12694..14040
                     /gene="asnS"
                     /locus_tag="VYN80_000910"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1540847.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            14193..14483
                     /locus_tag="VYN80_000911"
     CDS             14193..14483
                     /locus_tag="VYN80_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1540848.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            14495..14965
                     /gene="ssbA"
                     /locus_tag="VYN80_000912"
     CDS             14495..14965
                     /gene="ssbA"
                     /locus_tag="VYN80_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1540849.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            14997..15236
                     /locus_tag="VYN80_000913"
     CDS             14997..15236
                     /locus_tag="VYN80_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1540850.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            15531..16931
                     /locus_tag="VYN80_000914"
     CDS             15531..16931
                     /locus_tag="VYN80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1540851.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            16933..17292
                     /locus_tag="VYN80_000915"
     CDS             16933..17292
                     /locus_tag="VYN80_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1540852.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            17346..18095
                     /locus_tag="VYN80_000916"
     CDS             17346..18095
                     /locus_tag="VYN80_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1540853.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            18085..18384
                     /locus_tag="VYN80_000917"
     CDS             18085..18384
                     /locus_tag="VYN80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1540854.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            18455..19390
                     /locus_tag="VYN80_000918"
     CDS             18455..19390
                     /locus_tag="VYN80_000918"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1540855.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            19477..19989
                     /locus_tag="VYN80_000919"
     CDS             19477..19989
                     /locus_tag="VYN80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1540856.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            20014..20622
                     /locus_tag="VYN80_000920"
     CDS             20014..20622
                     /locus_tag="VYN80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1540857.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            20723..20905
                     /locus_tag="VYN80_000921"
     CDS             20723..20905
                     /locus_tag="VYN80_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1540858.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(20951..22396)
                     /locus_tag="VYN80_000922"
     CDS             complement(20951..22396)
                     /locus_tag="VYN80_000922"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1540859.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            22502..24124
                     /locus_tag="VYN80_000923"
     CDS             22502..24124
                     /locus_tag="VYN80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1540860.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT         7718 a         4429 c         5424 g         6618 t
ORIGIN      
        1 tttatttaat aaatatatgt tataatagag taaattaaga ctaatggagg acaaagaaat
       61 gggtttttat ttgatggttg cttcaatgct actcggactg ctcgctttga agataggttt
      121 ttctcagttc aaggagaaaa aagataaatt cttatctatc ttaacaagtt tagctggcac
      181 agcccttgtt ctcgtggctg tctggttagg atggcccaaa taaatagaaa aacctcggtc
      241 aaaccgaggt ttttcaaatg aatttcttgg ttgtggctaa aaaatatgct acactatcaa
      301 tatgaaaatt ttaatcccaa cagcaaaaga aatgaacaca gacttgccaa gtatcgaggc
      361 aattccttta aaaccagaaa gtcagaccgt gcttgatgcc ttggctctct attctgccag
      421 tcaattggag agtttctaca aggtatcagc tgagaaagcg gcggaagaat ttcaaaatat
      481 ccaagctttg aaaaggcaaa ctgctcaaca ctatacagcc ttgaaacttt ttgatgggct
      541 tatgtaccgc aacattaaga gagataagct gaccgaggcg gaacaagatt atcttgaaaa
      601 tcatgtcttc attacctcgg ctttgtacgg tgttgttcca gccttgtcac ccatggctcc
      661 tcaccgtttg gattttttga tgaaattaaa agtcgctggt aagactttga agagccattg
      721 gaaggcagtc tatgatgaag ctctgaagaa ggaagaagtg attttctctc tcttgtcatc
      781 agagtttgag actgtatttt ctaaggaaat cagagcaaag atggtgacct tcaaattcat
      841 ggaggataga ggcggtcagc tgaagattca ctcaactatc tccaagaaag cgcgcggggc
      901 ctttctaaca gctttaatag aaaatcaagt acaaactgtg ggggaagcac gtcgcttgaa
      961 ctttgctgga tttgtttacc gagaagattt gtcacaacca cagggattgg tttttgtaaa
     1021 ggaagtatag aaatcagata agagaaaagt cagcacgatt atgctgactt ttttgataac
     1081 ttataaagta ttggacaaat gaatctcatt tgataaggtg ttaagatgat taaccaaatt
     1141 catgattgac agatgatttc ggcaaataaa cttcttggta atccctggaa tatagaaatt
     1201 agacacgatg atatcgtaat ctgtctgatt taaaatctca ggacttgttt ttaattcatc
     1261 ccaaatatcg aaggtaaagc ggttattaca gtaataggaa agcatatcaa caaaggtaag
     1321 ggatatagca tggtcaaagt tactgataat caaaaccttg ataggtggtc gattttctaa
     1381 caactgagta gagaggtttt cagcatgggt taagatggtg tacatcaagt gttccaactg
     1441 ttcaggatgg tcatgctgtc caattgcctg acggtattga gccagttctt gacgcgcact
     1501 tcccataaag tctgggaagt aaacttcaaa ttttttaatc gtcaatgctt tttgttcaaa
     1561 taagattggg gtagaaaaga tttcttgtct ttcaaagaaa gcagtgttgt gtaaatgcca
     1621 aatcagctca tcgtggttag taaaagtaat cttatattgt tttgataggc gttctaatat
     1681 ttgactaagt aattggtaag agtatcttgc ttgactgtta tcttctaaag agttgaagaa
     1741 ttcttgagga tctaggaaaa tatcattctg aagaaaagaa ataaaaattt gagcgattgt
     1801 atctggagtc atttcaagcc caaaatgttt agagaaataa gcaagtgtct cctggaaatt
     1861 tggaatgctc tttaaaagag gataatattc tttgtaaaag tggttgggga ggtcaatgaa
     1921 gtgaccattt ttaaccctgt ggatactaat agctatcatc agtttgtaca ttctataaat
     1981 tgaaaatcgc attggataat tcgtgatttt atagaagaag tcagcaaact ctgttaaatc
     2041 ttcctcagga aggtcaggaa aaggccaatc taggaaataa tagcgctcag aaaagtattg
     2101 ggcaaaaaag taacgaatat cgatctcatt tccacgtatc tcggatggag taaaagataa
     2161 ttcaatttga aactgagatg aaagaacctt tgtaatattt ctgcccagtc gatagaggtt
     2221 ggcactacta aagtaaagat tttctattgt tcgataaata ggtagcccct cattgaagaa
     2281 catatactct agtaattgaa aagattgaga attagcaaga aaatattgat aaatatcttc
     2341 tacactagtg ttaacttcta aatctatcat gattccgtta acagaagatt ggatgttaat
     2401 agatggaaag gcaatacgta attcatttaa atcacttttt aagattcgtt ctgtacatcc
     2461 taatttttcg gctagttcat gtaatttcat ccaattacga ttctgaatta gggtttccat
     2521 taagcgtaat tgtctttgaa cttttgtgga taaaaggttt ctcatacggg tatttcctcc
     2581 ataattcatt atatcttttt ttgtctaagt agtataaaaa aattagagca agaaaattgt
     2641 cgttagtaat cggaaaaatt agaataatat aagtggcttt taagtgcaga aacagagtat
     2701 attaattgtt cagaaaatat tgattaatgc ctcaaatagt tgtacattgt aaactataaa
     2761 atactatcta ggagtttaaa atgaaacatt cacataaaaa atcatttgac tggtatagca
     2821 tgcaacaacg ttattctatt cgtaagtatt actttggtgc agctagtgtc ttgctcggta
     2881 ccgctttggt attaggtgta gcagctagtg tccaaacagt acaagcggaa gaaaacaaac
     2941 aagaaactac caatagtatt tctgttggta ggggagaagc agctactaaa ccagcagagg
     3001 tttctgcgtc taataaagag aaaacctatg cagctccaac tgtagctaat ccagtagaaa
     3061 cgactccagt taaaactgaa gaagttacta aaccagcaga aaaagttgaa gaagcaaaag
     3121 acaaaaaaga ggaagtaacg catcaagatg ccgttgacaa gtcaaaatta ttaacggctc
     3181 tttcgcgtgc taaaaaatta gaaagcaagt tatatacaga agcaagtgct gctaacttgc
     3241 aaacaagtat ccaagctggt caaagcttgc ttggaaaagc agatgcaact gaagctgaat
     3301 tatcagcagc agagtcatct attcaatcat ttattattgg tctagaactt cgttctaact
     3361 ctaataaaga aactgtatca gaaacgcctg tagcgaagaa agctgatgca gttgaatcaa
     3421 aagaaggggc taaaccagct gcaacaactg aacgttcagc tgttgatagc gctattttgc
     3481 caactagcac agctgacaaa gtagaaacaa cttcagctcc agcatctatt aatgaaatct
     3541 tgaaactagg tttgagcctt tctgatgctc gccaaaatcc agctatccgt aaggaaaatg
     3601 ttaatagagg gtatagtggt tttagagcgg ctagcaatcc agccaaccca attgtctcag
     3661 gttctggaaa tacagttgca tttgcagata ttagccaagg tggtcgtagc tatagtttcc
     3721 gtggctacgg gaactcacgt ggtggaaatt ctattcatta cgatgtaaca acagtacgta
     3781 gtggtaatag tgttaacttt acaattagtt attctgcgcc aggagattct agagagtttg
     3841 ttaataataa ttttatcttg gataaagggg atggatttgg aaatccttca aatgcaacga
     3901 tcacaagttc aaatccaaga gtaagggagc aatcaaaatc tattagtcag ggtgccaact
     3961 acgtatctca ctcaggatac agtatgacct ctgctatttc aacaaatacg gaacaaacta
     4021 tcagatttag tttgcctatc attaatctaa atggtgattt gtctgttcgt ttgaaacctg
     4081 ttacttttaa cgtggaccaa ggtggtggtg gtgccgctac tagcaatgac ccatacagta
     4141 actctaacta ttattataga gcaaacccac tatacttgga tgcaaaccct tatggtggta
     4201 ctaataataa gactgtttca gaagacattg acttccaaac tgtctatctt ccaactagta
     4261 agttaccaga aggtcaaact agattagttc gagaaggtga aaaaggacaa cgtcaaatta
     4321 cctataaagt tcatcgattt ggtaacgaaa cacttttagg attgccgatt agtaatagtg
     4381 ttactaaaga agctaagcca cgtattatgc aaattggtgt ggctaaagat ctaatcgata
     4441 cagtaaaacc acgtgttgat caaaataaag tcggtgatac aaataacctc actttctatc
     4501 ttgataacga tggaaacggt gtttatactg aaggtgtaga cgaacttgtt caaaaaattg
     4561 ctattaaaga tggagctaaa ggtgaaaaag gagaccaagg tgaacgcggt ctaactggag
     4621 ctaaaggtga aaaaggagac cgaggcgaac gcggtctaac tggagctcaa ggagctaaag
     4681 gtgaaaaagg agaccgaggc gaacgcggtc taactggagc tcaaggagct aaaggtgaaa
     4741 aaggagccca aggtgaacgc ggtttaactg gagctaaagg tgaaaaaggg gaccaaggtg
     4801 aacgcggtct cactggagcc caaggtgaaa aaggagaccg aggcgaacgt ggtttaactg
     4861 gagctaaagg tgaaaaagga gcccaaggtc aggcaggtcg tgacggtgta actccaaccg
     4921 taaccgttaa agataataaa aatgacggca ctcatactat cactattaac gacggtagag
     4981 gtaatgttac aagtactgtt gtaagagatg gtttcgatgg cgcaagtcca ttagttgcga
     5041 ctcaacgaaa tgacgcagat aagacaacaa ctgttatctt ctattacgat aaaaatggaa
     5101 acaatgaatt agatgcttct gataagaaat taaaagaagt tgtaattgca gatggtgcta
     5161 aaggtgaaaa aggggacaaa ggtgaacaag gtcttcaagg gcgtgatggt gaacaaggac
     5221 caaaaggaga agatgggaaa accccaacag ttaaagtaac tgatggtcaa gatggaacgc
     5281 atacaattac aattaacgat ggtaaaggtg gtataactac tacagtagta agagatggat
     5341 ttgatggtgc aagccctctt gtttctactc atagaaatga agcagataaa acaacaactg
     5401 ttattttcta ttatgatcta aatgataata atcaatttga tgaaggagat acaaaactta
     5461 aagaagttgt tatcgcagat ggaaaacaag gaccaaaagg tgacaaaggt gataacggaa
     5521 aagatggatt cacaccagaa gtaacagtta cagataacaa taacggaaca cacaccatca
     5581 caatcacaca accagacaac agaccatcat taacaacaat cgttaaaaac ggtgaagatg
     5641 gaaaaacacc aaaagtcaaa gcagaacgag atgatgcgaa gaaacaaaca acattaacat
     5701 tctacattga caaagatgga gatggaagtt acacagcagg aaaagacgag ttagttcaaa
     5761 caacagtagt taaagacgga caagacggag ctgcaggagc atctggacgt gatggaaaag
     5821 aagtattaaa cggaaaagta gacccaacaa cagaaggtaa agatggagac acattcgtaa
     5881 atacacaaac aggagatgta ttcgttaaga aaggtaacac ttgggaacca gcaggaaaca
     5941 tcaaaggacc gaaaggtgac aaaggtgcag atggtgccaa gggtgaaaaa ggagcccaag
     6001 gagaacgcgg cctgactgga gcgcaaggtg tcaagggtga aaagggagat cagggtgaac
     6061 gcggtctaac tggagcgcaa ggtgccaaag gtgacaaagg tgaacaaggt cttcaaggtc
     6121 gtgatggagc tcaaggacca aaaggcgcag atggacaaag aggaccagcg ggaccacaag
     6181 gaccaaaagg agaacaaggt aatccaggaa ctccaggtaa agatggaaaa tctctaattg
     6241 ctgttaaaaa tggaactgaa acaaaagtct atgtagagga tccggctaga ccaggacaac
     6301 cacttaatcc aaaccaacca ttagcaacta tcactgatgg aagaaacggt actaatggac
     6361 aatcaccaac aattacagcg acacgaagtg tgcaaaatgg caaaaatggt gtattagtaa
     6421 caattacacc agtaggaggt cgtccacaaa caacatttgt agaggatgga caaaagggtg
     6481 ctgatgggaa aaccccaaca gtaacaataa ctgaggggca aaacggcaca catacattaa
     6541 cggttcataa tccaggaagt ccagatgtga caactacgat ccgtgatgga gctacaggac
     6601 aagcaggtcg tgatggtaaa gatgtattaa acggaaaagt aaatccacaa ccaaaccaag
     6661 gtaaaaatgg agataaatat attaataccg aaaccggtga tgtctatgtt aaaaacaatg
     6721 gaaactggga taaagaaggc aacatcaaag gccctaaagg ggacaaaggt gcagatggtg
     6781 ctaaaggcga aaaaggagac caaggcgaac gcggcctaac tggagcgcaa ggagctaaag
     6841 gtgcggatgg cgcagtaggt cgtgatggac gtgacggtaa agacgtgttg aacggcaaag
     6901 ctaacccaga agcacatcaa ggtaaagacg gcgataaata cgttaataca gaaacaggcg
     6961 acgtcttcgt taagaataac ggcaactggg ataaagaggg caacatcaaa ggccctaagg
     7021 gtgacaaagg tgaacgcgga gaagatggta agactccaga agtaactgta actccaggta
     7081 aagatggcca tagtactgac attacattca ctgttccagg taaagatcca gttacagtaa
     7141 atgttaagga cggagaaaat ggtctgaacg gtaaaactcc aaaagttgat ttacttcgtg
     7201 tccaaggaaa aaacggaaat ccatctcata caattgtgac attctataca gatgaaaaca
     7261 atgacggcaa atatacacca ggaactgatg aacttctagg ttcagaaatg attaaagatg
     7321 gtgctaaagg cgcggacgga cgagatggta aatcattgct tactgtcaag gatggtaaag
     7381 aaactaaagt ttaccaagaa gatccagcta acccaggaca accattaaat ccagaaaaac
     7441 cacttgcggt aattagagat ggagtagatg gaaaatcacc tacagttaca gctgttcgta
     7501 aagatgaagc agggcataaa ggtgtagaaa tcactgttga taaccatgat ggttcacaac
     7561 caactacagt ctttgttcaa gatggtgcta agggagaaac tggtgcaacc ggtcaggatg
     7621 gacaaactcc tacaatcact actcaacgtg gacaagatgg ccaaagcact gttgtaacta
     7681 tcacaacatc aggtaaagat ccagtaacct tcactgtaaa agatggtaag aatggtaaag
     7741 atggccgtgc accgaaaatc aaagtagaag atattacttc accttcaaga attagacgcg
     7801 atacagatgc tgctgcaact ccaacgcgta acggtatccg tgttacagtt tatgatgatg
     7861 ttaatgacaa tggggtatac gacgaaggtg tcgataaagt attaaatagt aaagatattt
     7921 ataacggtat agatggacgt gatggttcag ctccaactat tactacaaaa gataatggag
     7981 atggaactca cactatcaca gttcaaaacc cagatggttc tgaatcaaca acagttgtta
     8041 aagatggtaa agacggtaaa actgcgaata tcactacaac agaaaaccca gatggaagcc
     8101 acacaattac ggtgacaaat ccagatggtt caactaaaga aactgttgtt aaaaacggta
     8161 aagacggtaa gactcctaaa gttgaagtaa cggataacaa cgatggaact catactgtta
     8221 aagttacaga tggagacggc aatgttacca acgctatcat caaagatggt aaagacggta
     8281 aagctgcaac agcaacaact actgaaaatc cagatggaag ccacacagta acaatcacta
     8341 acccagacgg aactaagaat gagtttgttg ttaagaatgg acgtgacggt gttgacggac
     8401 gtactccaac cgcatctgtt cgtgataatg gagacggaag tcatacaatc gttattacaa
     8461 atccagaagg tgtgacaact gaaaccacag ttcgtgatgg taaatcacca aaagtgacta
     8521 taactgatga acaaaatgga actcataaga tctctgttct aaatggtgac ggaacaacta
     8581 caacaactac agttcgtgat ggtagagaac caaaacttga agttattgat aacaacgatg
     8641 gttcacacac tattaaagtg acaggtgctg atggtaaagg aacgacaact acaatctttg
     8701 atggtaaatc accaaaagcg aacatcgttg ataacggaga tggaactcat acattaacaa
     8761 tcgtagattc tgatggtcgt gaatacaaat ctattatcaa agatggtaaa gacggcaaag
     8821 atagcgtttc accaactgta actgttaaaa ataataacga tggaactcac gttgttacaa
     8881 tcactaatcc agatggaagt aagacagaaa tggtgattaa agacggtaaa gatggtaaat
     8941 caccaaaagt ttctgttgaa gataatggtg atggtagtca tacaatcaca atcatcaatt
     9001 ctgatggaac tgtgacaaaa acagttatta aagatggtag agatggacgt gatggtcgag
     9061 acggcaaaga cggtaaagac ggtaaagatg gaaaatgtgg atgccaagac aaaccagtaa
     9121 caccatcaaa tgacaaacca gttcctccaa caccaaatgt gccgacacca gaagtaccgg
     9181 ttaaacctgt gccagcgcaa ccaacaccaa atgtaccgac accagaagta ccggttaaac
     9241 ctgtgccagc gcaaccaaca ccaaatgtac cgacaccaga agtgccagta caaccaactc
     9301 cagctgttcc aacaccagaa gtaccggtta aaccagtacc agcggttcca gaacaaccag
     9361 tagtaccaac accggctcaa ccagcaactc cagtaaatgc taacccagta gcaccaacta
     9421 caggtaaaga aaaccgtggg gacaaattac ctgaaactgg aagccaatct gattatatct
     9481 ctgttctttt aggtagcggt attctattga gcctatatgt aggacgaaga aaagaagctt
     9541 aatcagccaa aagcagtcac taatttctcg aaagaagatg ataagtgata gatctcgaat
     9601 gaaagaatat tcttagaatg attttcctat gatggaaaag actgctaaaa accttgagtg
     9661 gtaacgctca aggttttttt ggatggctga tgtttgtctg aaaatcttga ttgagaaata
     9721 aaaagccacc ctattaggtg gctataattg atttagactt tctaaaattt tctatttttt
     9781 actgaatcgc ttcgaccaag tcttgagctt gtttttcaag tgagtttagg actgtttctt
     9841 caagaaccaa ttttccgtct gcccaggcag agtcattaac acgtgcagca gtgaaatcac
     9901 caacgccttg tgtacggata aatggcaaga ggtctttgta gatagcgaaa agttgatcgt
     9961 gccctgcatt ggctacagat gatacggtga caaacttgtc ttgaagggca gaaacgccac
    10021 gcgtatcaga caagtcaagg gcacgagata gccagtcaag caagtttttt actgtaccag
    10081 ggatagagaa gttgtagact ggagagaaaa tccagatagc atccgcaacg agaactgctt
    10141 cacgagcagc agctacagct ggatgtgttg gaacttccaa atcttggctg aagagaggaa
    10201 gggctgaata atcaaggtag ctaacttccg ctttaccagc aagtgctttc tcagcttcga
    10261 gcgccatttg gtggttgaaa gaaccttgac gtagtgatcc gacgataaat aatacttttt
    10321 tagacatgat gtgtctcctt tttcttcaaa ttttgagaac tctctcgttg ttataaaata
    10381 cattacaaca aaacaaaaga acttgcaaga aatttgctca gagtttattt gaaaattgta
    10441 aaatttcgga aactctagtc atttgtgact gaaaaagtaa gaactgtttc actttcaaaa
    10501 tcggttttgt ttgaagggag aaatagaatt ggttgctcaa agcacagctt tgaggttgca
    10561 gataaagctg acgtggtttg aagagatttt cgaagagtat tcttatattc acttgtaata
    10621 aagcttaata gatggtaaaa tagacgagtg aaaaaaagac aaaacaaagc aaaaaataat
    10681 ctactgtggc agtatggtct agggatgacg attttgtttg tggttatcag tgcttccttt
    10741 ctgtatatgg tttctcttag catgaaaccc tatcaaacag ctaaaagtga aggagaaaaa
    10801 ttagctcagc agtatgcagg attagagcag gccgatcagg ttgatttata caatggcttg
    10861 gaatcttatt acagcgttct tggtcgtaat aaacagcaag aagcacttgc tgttctgatt
    10921 ggaaaagatg atcataagat ttacgtttat cagctaaatc agggtgtttc acaagaaaaa
    10981 gcagaaacgg tttctaagga aaagggagct ggcgaaattg acaagattat ctttggtcgt
    11041 tatcaagata agccaatctg ggaagtcaag tcaggatctg atttttatct agtagatttt
    11101 gaaacaggag cattggtcaa caaggagggc ctatgaaatt atccaaccgt gttttagaaa
    11161 tggaagaaag tgtgactttg gctgctggag cccgtgccaa agcactgaag gctgagggta
    11221 gagatatttt gtctctaacc ttgggtgagc cagatttcac tactcccaaa aatatccaag
    11281 atgccgccat tgcatcgatt cgagatgggc gtgcttcttt ttatacagta acctcaggtc
    11341 tgccagagct taaggcggcg gtcaatagct actttgagcg cttttacggc tattctgtag
    11401 cgtcaaatca agtgacagtc gctgcgggag ccaaatattc tctctatacc ttctttatgg
    11461 ctgtggtcaa tccaggtgat gaagtcatca tcccaacccc atactgggtt agctatggag
    11521 atcaggtcaa gatggcagaa ggcgttcccg tctttgtttc tgctaaggaa gacaatcact
    11581 ttaaggtgac cgtagagcag ttagaagcag ctcgcactga caagaccaag gttttggtgc
    11641 tgaattcgcc atctaatccc acaggtatga tttacacccg tgaggaactc ttggcaattg
    11701 gaaactgggc tgtagaaaat gatattctca tcctagcaga cgatatctat ggccgcttgg
    11761 tttataatgg tcatgagttc acaccgattt ctagcctatc ggaagcgatt cgcaagcaaa
    11821 cagtggtcat caatggtgtg tctaaaacct atgccatgac tggttggcgg attggttatg
    11881 ccgttggaga agcagacatt atcgctgcca tgtccaagat tgcaggtcaa acaacttcga
    11941 atccgtcagc agtagcccaa tatgcagcag ttgaggctct atcaggtgag caagatactg
    12001 tagaaagcat gcgtcaggcc tttgaggaac gtcttaatac catctatccc ctccttgcag
    12061 aggtgccagg atttgaagtg gtcaaaccgc aaggggcctt ctatctcttc ccaaatgtca
    12121 aaaaggccat ggagatgaaa ggctacacgg atgtgacaga ctttacaact gctatcttag
    12181 aagaagccga agtggccttg gtaacaggag ctggatttgg agcaccagaa aatgtccgcc
    12241 tcagctatgc gacagaccta gacacgctta aagaagcagt cgaacgcttg aaagcattta
    12301 tgggtagtga gaatgattga tcattttgag attaaggtaa aggatttaca aatttcagaa
    12361 ggattttata ggagttttct cgctcctttg gactataaat tggcttttaa gactagttct
    12421 ctaattagtt ttctatcccc gaacagccct catcctggtg gtgatttttg gctgactcag
    12481 ggaacacaag atcctgttca ttttgctttt ttagcagaaa ataaggaaga agtttaggct
    12541 tgttatgagg ctggcttaga ggcaggtggg cgagataatg gggctcctgg ttatcgaagt
    12601 gagcgtccga tttactatgc tgcttttatg attgacctgg atgggaacaa tatagaggtg
    12661 gtttgccata aagaataaaa aagaaaagga aaaataatga caaaacgtgt aacgattatt
    12721 gacgtaaaag actatgttgg tcaggaagtg acgattggcg cttgggttgc caataaatca
    12781 ggaaaaggga aaatcgcttt cttacaattg cgtgatggaa cagccttctt tcaaggtgtg
    12841 gcttttaaac caaactttgt cgaaaaattt ggtgaagaag tgggacttga gaagtttgat
    12901 gttatcaaac gcttgagcca agaaacgtct gtttatgtga caggtattgt caaagaggac
    12961 gaacgttcta aatttggcta tgagttggac atcacagaca tcgaagtgat cggtgaatct
    13021 caagactacc caatcacacc aaaagaacac ggaacagact ttttgatgga taaccgtcac
    13081 ttgtggctac gctctcgtaa gcaagtagct gtgttgcaaa tccgtaacgc tattatctat
    13141 gcaacttatg agttctttga caagaacggt tttatgaagt ttgacagccc aattctttca
    13201 ggaaatgcgg cagaagattc tacagaactc tttgaaactg actacttcgg aacgccagcc
    13261 tacttgagcc aatcaggtca gctttaccta gaagcagggg ctatggctct tggtcgtgtc
    13321 tttgactttg gtccagtttt ccgtgctgaa aaatcaaaaa cacgccgtca cttgactgag
    13381 ttctggatga tggatgctga gtactcatac ttgacacatg atgagtcgct tgacttgcaa
    13441 gaagcttatg tgaaagctct tctacaaggt gttcttgacc gcgcgcctca agccttggaa
    13501 accttggaac gtgatacaga actcttgaaa cgctacattg cagagccatt caaacgtatc
    13561 acttacgatc aagccattga cctcttgcaa gagcatgaaa atgatgaaga tgctgactac
    13621 gagcatcttg agcatggtga tgactttggg tcaccacacg aaacttggat ttcaaaccac
    13681 tttggtgtgc caacatttgt catgaactat ccagtagcca tcaaggcctt ctacatgaaa
    13741 ccagttcctg gaaatccaga gcgcgtgctt tgtgcagact tgcttgctcc agaaggctat
    13801 ggagaaatta tcggtgggtc tatgcgtgag gaagattacg atgcccttgt cgctaagatg
    13861 gatgaacttg gcatggatcg tacagaatat gaattctacc ttgaccttcg taaatacggt
    13921 acagttccac acggaggatt tggtatcggt atcgaacgta tggtaacctt tgcagcagga
    13981 acaaaacata tccgtgaagc tattccattc ccacgtatgt tgcaccgtat caaaccataa
    14041 gaacaggaaa acgcccatgt ggcgtttttt cgaattctag tgtttttact tgcgtaaaaa
    14101 aattttttct agtataatag tttattgtga gcgaacctca cttacccctt gcaaagtctt
    14161 ggggtcatta gaccaaaagg aggaacatat caatggctaa atacgaaatt ctttatatca
    14221 ttcgtccaaa cattgaagaa gaagctaaaa acgctttggt agcacgtttt gactctattt
    14281 tgactgacaa cggtgcaact gttgttgaat caaaaacttg ggaaaaacgt cgtcttgcat
    14341 acgaaatcca agatttccgt gaaggacttt accacatcgt taacgttgaa gcaaatgacg
    14401 atgcagctct taaagagttt gaccgtcttt caaaaatcaa cgctgacatt cttcgtcaca
    14461 tgatcgtcaa aattgacgcg taagaaggta aactatgatt aacaatgttg tacttgtagg
    14521 gcgtatgaca cgtgacgctg agttgcgtta taccccatca aatgtagcag ttgcgacttt
    14581 tactcttgca gtaaaccgta catttaagag tcaaaatggt gaacgtgagg ctgattttat
    14641 caatgtcgtt atgtggcgcc aacaggctga aaatcttgct aactgggcta aaaaaggctc
    14701 acttatcggg gtgacaggtc gtatccagac tcgtagttac gataaccagc aaggacaacg
    14761 tgtctacgtg acagaggtcg tggctgagaa tttccaaatg ttggaaagcc gtagtgtgcg
    14821 tgagggtcac acaggtggag cttactctgc accaactgca aactattcag cacctacaaa
    14881 ttcagtacta gacttttcac gtaatgaaaa tccatttgga gcaacaaacc cattggatat
    14941 ttcagatgat gatttaccat tctaatggac aactaaactt ataaaggaga aaaaacatgg
    15001 ctcaacaacg tcgtggcgga ttcaaacgcc gtaaaaaagt tgattacatc gcagcaaaca
    15061 aaattgaata tgttgattac aaagatactg agcttcttag ccgtttcgtt tcagaacgtg
    15121 ggaaaatcct tcctcgtcgt gtaacaggaa cttcagctaa aaaccaacgt aaagtaacaa
    15181 cagctatcaa acgcgctcgc gtaatggctt tgatgccttt cgtaaacgaa gattaaagaa
    15241 aagacccggt tgggtctctt tttcatgagc ttgaaaacaa gaaagcgaat taagacccca
    15301 atgggtcttt tttcacaaac tttgaaatga gagagcgagt taggctcagt ggctcccttt
    15361 ttacgagttt gaaaacaaga gaacacatta aaagcacgat ggagcttttt cataaatatg
    15421 tcttttttct catccaagaa attaaaataa aaatgataaa ctttttgaaa ataggggatt
    15481 tgcctacttt atgatataat ggaaagagtt agattgaaag aggtagtgag atggacgcga
    15541 aattaagata caaggcaaag aagatcaaga ttgtcttttt tgatattgat gatacattgc
    15601 ggaattcaaa gacagggttt attccaacta caattcccac tgtttttaaa cagttgcgtg
    15661 aaaaaggaat tttaacagga atcgcctctg gacgtggcat ttttggtgtt gttccagaga
    15721 ttcgtgatct caagcctgac ttttttgtaa ctttgaatgg ggcttatatc gaagataaaa
    15781 aaggtcaggt catttatcag catcagattg aaaagtcaga tgttgaggag tatatctctt
    15841 gggctaagca agaaggaatt gagtatggct tggttgggag tcatgatgcc aagttgtcga
    15901 ctcgcaccga tatgatgagt gaagctatca atccaattta tcccgactta gatgtagatc
    15961 ccgatttcca tgaaaaagaa gatatctatc agatgtggac ttttgaagat aagggagatg
    16021 acttgcactt gcctgacagt ctctcagaca aacttcgcat ggttcgttgg catcaacatt
    16081 cgtctgatat tgtgccgatt tcaggctcca aagcgacggg ggtggaaaaa gttgtggaac
    16141 accttggctt gaaaccagag aaggtcatgg tttttggaga tggtctcaac gacttggaac
    16201 tctttgatta tgctggaatc agcgttgcca tgggaatttc tcatgataaa atcaaagaaa
    16261 aagcagatta tattacaaaa acattagaag aagatggcat ttttgatgcc ttagaagtat
    16321 ttggtatggt agaaaaagaa ttgcattttc cacaagtaga cattgaaaca gtagaaggtc
    16381 ctcttgcgac tattaagacc aatcacggag acttacgtat caagcttttc cctgaacatg
    16441 ctcctaaaac agtggctaac tttgtatctc tttcaaaaga tggctactat gatggtgtca
    16501 ttttccaccg tattatcaag gactttatga tccaaggtgg aaacccaact ggaactggta
    16561 tgggtggcga gtcaatctac ggcgaatctt ttgaggatga attctcagaa gagctttaca
    16621 atatccgtgg tgctctttcc atggcaaatg ctggtccaaa taccaacggc agccagttct
    16681 ttatcgtgca aaaccaacac ctaccttatt ctaagaaaga aattactcgt ggtggttggc
    16741 cagaaccgat tgcagaaatc tatgccaatc aaggtgggac acctcaccta gaccgtcgtc
    16801 acacggtttt tggtcagtta gctgatgaag catcttacgc tgtcttggat gccattgctg
    16861 ctgttgagac aggagctatg gacaagccag ttgaagatgt tgtaattgaa actattgaaa
    16921 tcgaggacta agatgaaaat cggtgataag ctaaaggggc gtattacagg gattcagccc
    16981 tacggtgcct ttgttgagtt agagacgggt gatacggggc tgattcacat ttcagaaatt
    17041 cggacaggat ttattgaaaa tattcatgaa accttgaaag tcgatgaaga ggttcaagtt
    17101 caggtagtgg atttagatga atttacgggg aaagccagtc tttctatccg cactttggag
    17161 gaagaaaagt accagtttcc tagacggaga cgcttttcaa gcgaccgctt taactacggt
    17221 tttgctccct ttcgacgaat gctaccaatc tggactggag aagccctgca ccatttaaaa
    17281 aagaagaagt agtttagagt tttatatttt ttgtaatgtt aatataaatt tgctataata
    17341 ggaccatgga agaagaacaa ttattaaaat caggagagcg cattaaccag ctcttttcga
    17401 cagatatcaa aatcattcaa aatagagagg tttttagcta ttcggtggat agtgttctct
    17461 tatcacgatt tccacgtttt cctaagaagg ggttgattgt ggatttctgt gctgggaatg
    17521 gagcagtggg gctttttgct agcactcgta ctcaagcaca gatattgtct gttgagattc
    17581 aggagcgttt ggcggatatg gctgaacgct ctgtccgttt gaatggatta gaggagcaaa
    17641 tgcaggtcat ctgcgatgat ttgaaaaata tgcctgctca cattcagggg agtaaggtgg
    17701 atatgatttt gtgtaatcca ccctatttca aggtggatcc ttattctaat ctgaacgaga
    17761 gtgaacatta tctcttggct cgacacgaaa tcacgactaa tttggaagaa atctgtcata
    17821 gtgcccagag tattctcaag tctaatgggc gtttggccat ggttcatcgt cctgatcgac
    17881 ttctggatat tttggatacg ttaaaacgac ataatctagc ccctaagcgc ctgcagtttg
    17941 tttatccaaa aagagaaaag gaagccaata tgcttttgat tgaggcgatc aaggatggct
    18001 caacaagtgg ctttaaggtc ttacctcctt tgattgtcca caatgatgat ggctcttata
    18061 cgcccgaaat cgaagagatt tattatggat cataaggctt atatgtacgt tctggagtgt
    18121 cgtgatggat cctactatat aggctatacg actgatatga gaagacgcct cgctatccac
    18181 aatagtggga agggagccaa atatacacga gcacgcttgc cagtcaaact tatctatgct
    18241 caaggttttg ccagtaagga agaagccatg tcggctgaag ctcttctcaa gcgtaagaag
    18301 aggccacaga aggaagaatt tttatctgaa aatcaagata gaaatttact cagttatttt
    18361 gaagaatcgt ggggagtcct ttgactcctc ttacctttgt caaaaagcga aaaaaatgct
    18421 acaataaaga aaataaacaa aatgaggtat tatcatgtcc aagattctag tatttggtca
    18481 ccaaaatcca gactcagatg ccatcggatc atctgtagct tttgcctacc ttgcaaaaga
    18541 agcttacggt ttggatacgg aagctgttgc ccttggaact ccaaatgaag aaacagcctt
    18601 tgtcttgaac tattttggtg tggaagcacc acgtgttatc acttctgcca aagcagaggg
    18661 ggcagagcaa gttatcttga ctgaccacaa tgaattccaa caatctgtat cagatatcgc
    18721 tgaagtagaa gtttacggtg ttgtagacca ccaccgtgtg gctaactttg aaactgcaag
    18781 cccactttac atgcgtttgg agccagttgg atcagcgtct tcaatcgttt accgtatgtt
    18841 caaagaacat ggtgtagctg tgcctaaaga gattgcaggt ttgatgcttt caggtttgat
    18901 ttcagatacc cttcttttga aatcaccaac aacacaccca acagataaaa tcattgctcc
    18961 tgaattggct gaattggctg gtgtgaactt ggaagaatat ggtttggcaa tgttgaaagc
    19021 tggtaccaac ttggctagca aatctgctga agaattgatt gacatcgatg ctaagacttt
    19081 tgaactcaac ggaaataatg tccgtgttgc ccaagtgaac acagttgaca tcgctgaagt
    19141 tttggaacgc caagcagaaa ttgaagctgc aatgcaagct gccaacgaat caaacggcta
    19201 ctctgacttt gtcttgatga ttacagatat cgtcaactca aactcagaaa tattggctct
    19261 tggtgccaat atggacaagg tcgaagcggc tttcaatttc aaacttgaaa acaatcatgc
    19321 cttccttgct ggtgccgttt cacgtaagaa acaagtggta cctcaattaa ctgaaagctt
    19381 taatgcgtaa gattttgggt gtcagctcaa aatcggaaag tctagtttgc cttatatcga
    19441 aaggagtttc ggctcctttt tttctaggag tgaagtatgt tagaaaatgg cgatttgatt
    19501 tttgtgagag atgggtcaga catgggacag gccatccaga cttccacagg taactatagc
    19561 catgttgcca tttatttgga tgggatgatt tatcatgcta gtggacaggc tggtgttgtc
    19621 tgtcaagaac cggcagactt ctttgagtcc aatcatttgt acgacctcta tgtttaccca
    19681 gaaatggata tccagtcggt gaaggaaaga gcttgcagac atcttggagc accctacaat
    19741 gcttctttct atccagatgc agctggtttc tactgctccc agtatatagc agaaatccta
    19801 cctatttttg aaactattcc tatgaaattt ggagatgagg aacaggagat tagtgatttt
    19861 tggagggagt attacataga actaggtctg cctgttcctc tgaaccaagc tgggaccaat
    19921 cctagtcagt tggcagcatc gcctctgtta caatgtaaag aaaggaatct tcatgattca
    19981 gatttttaat ccatctcgtt tgacgagaca gccatttttg gagaattgat ccgctatctg
    20041 gaccagtatg aggatgtgat tctacgggaa attaaggctc aatttccaga tgttgcagtt
    20101 gataaactca tggaagagta tataaaggca agcttgattc tacgtgaaaa taagcgctat
    20161 taccttaatt ttcctacgct tgaatcactt gatagtcttg aactggatca agagattttt
    20221 gtcagagaag ctagtccggt ctatcaagcc ttgttggagc agagttttga gacggaattg
    20281 cgcaatcaaa tcaatgcagc tattttagtt gaaaagacgg actttgcgcg cattaaaatg
    20341 accctgtcca attattttta caaggtcaaa cagcagtatc ctttgacaga aaaacagcag
    20401 gagctctatg acattttagg agatgttaat cctgagtatg ccctcaagta tatgacggct
    20461 tttttgttga aatttctcaa aaaagaccag cttatgcaga aatgccgtga tatctttgtg
    20521 gacagtttgg ttgtcttagg ctatattgtg caaaatgaag atggaaagta tgagttggct
    20581 atcgattttg ataaggagag attaactttc tacttagcgt gatttcttgt ttctgagtac
    20641 attgtttgac tttccttagt attcggtata aactatatgt aaccggtaac acatatcgga
    20701 ataaactaaa ggagacaatc atatgtcact tgaaaacaaa ttggaacaag caacaggcgc
    20761 tgtcaaagaa ggttttggta aagttactgg agacagcaag acagaacttg aaggagctgt
    20821 tgaaaaaaca gttgctaagg caaaagacgt tgtagaagac gcaaaaggtg ctgtagaagg
    20881 tttgaaaaac gtttttacta aataatagga aaaaatcaag ggtttcattt tcccttgatt
    20941 ttttctattc ttataaataa ttttctgcga cggctgtatc tcctgggtag gattctttct
    21001 tgccctggat gatttggtaa caatcgactc ccttacccgc aataataact gcatctaatt
    21061 cgtgatttgt gatagccatt gccgccttga tggcttcttg gcgatccgca atcttttcaa
    21121 caggatgatt gatgtagcta ctaatttcat ctgcaatggc cattgggtct tcatagttag
    21181 ggtcatcagc agtcagaaag acttgaatct cagggtgttg attgaggagg aggccaaagt
    21241 ccttacgacg actttctccc ttgtttcctg ttgatcccag aaccagagca atctttccgg
    21301 tttgatgagt ttccaccaca ttgatgagtt ttttcagact atccccattg tgggcatagt
    21361 cgatgaagac cttggctcca tttttctgag tgaggacttc catacgacca ggaacgcggg
    21421 ttgcagcgat gccttttttg atgtcctcaa gacttgctcc gagacggaga caagcaagtc
    21481 cagcagcaac tgcattttct tggttgaagt tgccaatgag ttggatatca taatctccag
    21541 cgagtttacc cgtagctgaa aagctaaagg ctttggaatt ctcgatttgg ttatcaaatt
    21601 ggctaccata gaaatcatgg tcttgatctt caacctgttc tttcaagact gagaagtggt
    21661 ccatgtcact gttaatgatg actgctcggc tattttccat caagagacgc ttgtggtaga
    21721 aatagtcttc aaagctaggg tgttcaatcg ggccgatatg gtctgggctg atattgagga
    21781 aaactcccac atcaaaggtt agaccataga cacgtttgac cagataggat tgactggaga
    21841 cttccatgat gaggtgggta cggtcatttt gcacagcctg attcatcatg tcaaagaggt
    21901 caatactctc aggggttgtc aacgctgact taaagaaagt ctcgccatca agagttgtgt
    21961 tcatggtcga caacatagca ggtctatgcc cttgagataa gatgttatag gcgaaatagg
    22021 ttgctgttgt cttaccctta gtaccagtaa aggcaaggag tttgagtttc tcttgtggat
    22081 taccatagaa ctccatggca atcaaactca tggctttctt tatatcgttc acaatgatga
    22141 cagggatatc gacttcgtag tccttttcag ctacatacca agctaaacct tgtgttatag
    22201 cagaaagaag gtattctttt ttaaaggcag cgccttttgc aaaaaaaaga gtgtcttctg
    22261 ttacttttcg gctgtcgtag ctgatgctat caaaaacaac tttgctgtag ttgtagtggt
    22321 aatgaccttg gtcaataatt tcgcgaaaaa ggccatcttt ctttaaaata tctaatacgg
    22381 tttcaatctt aatcatactt tctattgtaa accgaaagtc gcaaatttac aagtaacaag
    22441 gaaaagttta taatggaaga taaggagttt ttcctagtta tcaaaattga atgaggaatc
    22501 tatgtcgcac gaaaacaatc accagcaggc ccagatgtta cgggggactg cttggctaac
    22561 ggctagtaac tttatcagtc gcctactcgg ggctgtttac attatccctt ggtacatctg
    22621 gatgggggct tatgcagcta aggcaaatgg tctctttacc atgggttaca atatctatgc
    22681 ttggttcttg ttggtttcaa cagcggggat tccagttgcg gtggccaagc aagttgccaa
    22741 gtataatacc atgcgagaag aagagcatag ctttgccctg attcggagct tcttaggctt
    22801 tatgacagga ctaggcctgg tttttgcttt agtcttgtat gtctttgctc cttggctagc
    22861 agacttgtct ggcgtgggca aagacttgat cccaatcatg caaagcttgg cttggggagt
    22921 cttgattttc ccgtctatga gtgttatccg aggatttttc caagggatga ataacctcaa
    22981 accctatgcc atgagccaaa ttgctgagca ggtcattcgt gttatctgga tgctcctagc
    23041 aacctttatc attatgaagc tcggttcagg agattatcta gcagccgtta cccaatcaac
    23101 ctttgctgcc tttgtcggta tggtagccag ttttgcagtc ttgatttatt tccttgccca
    23161 agaaggttca ctcaaaagag tctttgaaac aggagataag attaacagta agcgtctctt
    23221 ggttgatacc attaaggaag ccattccttt tatcctgaca gggtctgcca tccagctctt
    23281 ccagattttg gatcagctga cctttatcaa tagtatgagc tggtttacca actacagcaa
    23341 tgaggacttg gttgtcatgt tttcttattt ctcagccaat cctaataaaa tcacgatgat
    23401 tttgatttct gtaggggttt cgattgggag tgttggtttg ccacttttga cggaaaacta
    23461 tgtcaagggg gacttgaaag cagcttctcg tctggttcag gacagtctca ccctactctt
    23521 tatgttcttg ctaccagcaa cggttggagt ggttatggta ggagaacctc tttatacggt
    23581 cttctatggt aagccagata gtttggctct gggcttattt gtctttgcag ttttgcagtc
    23641 tattatttta ggcttgtaca tggtcttgtc tccaatgctt caggccatgt tccgcaaccg
    23701 caaggccgtt ctctatttta tctatggttc tattgccaag ctagtcttgc aactacctac
    23761 catcgccctc ttccacagtt atggtccttt gatttcaaca accattgctc tcatcattcc
    23821 taacgtcttg atgtatcggg atatttgtaa agtaactggt gtcaagcgca aggtgatttt
    23881 gaagcgaacc attttaatca gtttgctgac cctagtcatg tttctgttaa taggaaccat
    23941 ccagtggctg ttaggatttt tcttccaacc aagtggacgt ttgtggagct tcttttatgt
    24001 agctcttgtc ggtgccatgg ggggtggact ttatatggtt atgagtctgc gtacctattt
    24061 attagataag gtaataggaa aagcccaagc agatcgcctg cgagcaaaat ttaagctttc
    24121 gtaaggtagc atttataaat aaaaggaata agttgaacaa tcttttagga gaacttgaaa
    24181 cttattcct
//
LOCUS       DAVLWA010000024        22710 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLWA010000024 DAVLWA010000000
VERSION     DAVLWA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.024"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(14..3190)
                     /gene="carB"
                     /locus_tag="VYN80_000924"
     CDS             complement(14..3190)
                     /gene="carB"
                     /locus_tag="VYN80_000924"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1540861.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLETGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3502..4581)
                     /gene="carA"
                     /locus_tag="VYN80_000925"
     CDS             complement(3502..4581)
                     /gene="carA"
                     /locus_tag="VYN80_000925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1540862.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4631..5554)
                     /locus_tag="VYN80_000926"
     CDS             complement(4631..5554)
                     /locus_tag="VYN80_000926"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1540863.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5573..6094)
                     /gene="pyrR"
                     /locus_tag="VYN80_000927"
     CDS             complement(5573..6094)
                     /gene="pyrR"
                     /locus_tag="VYN80_000927"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1540864.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6305..6934)
                     /gene="nth"
                     /locus_tag="VYN80_000928"
     CDS             complement(6305..6934)
                     /gene="nth"
                     /locus_tag="VYN80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1540865.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6934..7476)
                     /locus_tag="VYN80_000929"
     CDS             complement(6934..7476)
                     /locus_tag="VYN80_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1540866.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            8201..9760
                     /locus_tag="VYN80_000930"
     CDS             8201..9760
                     /locus_tag="VYN80_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1540867.1"
                     /translation="MHYEHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSEAADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9804..10703)
                     /gene="htpX"
                     /locus_tag="VYN80_000931"
     CDS             complement(9804..10703)
                     /gene="htpX"
                     /locus_tag="VYN80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1540868.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10705..11265)
                     /locus_tag="VYN80_000932"
     CDS             complement(10705..11265)
                     /locus_tag="VYN80_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1540869.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11360..12073
                     /gene="rsmG"
                     /locus_tag="VYN80_000933"
     CDS             11360..12073
                     /gene="rsmG"
                     /locus_tag="VYN80_000933"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1540870.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHLYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12376..13659
                     /locus_tag="VYN80_000934"
     CDS             12376..13659
                     /locus_tag="VYN80_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1540871.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13854..15425)
                     /gene="ffh"
                     /locus_tag="VYN80_000935"
     CDS             complement(13854..15425)
                     /gene="ffh"
                     /locus_tag="VYN80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1540872.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMSDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15437..15769)
                     /locus_tag="VYN80_000936"
     CDS             complement(15437..15769)
                     /locus_tag="VYN80_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1540873.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(15860..16237)
                     /locus_tag="VYN80_000937"
     CDS             complement(15860..16237)
                     /locus_tag="VYN80_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1540874.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKRDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYILTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16309..17613
                     /locus_tag="VYN80_000938"
     CDS             16309..17613
                     /locus_tag="VYN80_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1540875.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17626..18432
                     /gene="yidA"
                     /locus_tag="VYN80_000939"
     CDS             17626..18432
                     /gene="yidA"
                     /locus_tag="VYN80_000939"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1540876.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGIHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTLVL"
     gene            complement(18487..18558)
                     /locus_tag="VYN80_000940"
     tRNA            complement(18487..18558)
                     /locus_tag="VYN80_000940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18523..18525),aa:Arg,seq:cct)
     gene            complement(18600..18947)
                     /gene="rplS"
                     /locus_tag="VYN80_000941"
     CDS             complement(18600..18947)
                     /gene="rplS"
                     /locus_tag="VYN80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1540877.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19066..19395)
                     /gene="crcB"
                     /locus_tag="VYN80_000942"
     CDS             complement(19066..19395)
                     /gene="crcB"
                     /locus_tag="VYN80_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1540878.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLILTYLGGLVA
                     IFLGILL"
     gene            complement(19389..19763)
                     /gene="crcB"
                     /locus_tag="VYN80_000943"
     CDS             complement(19389..19763)
                     /gene="crcB"
                     /locus_tag="VYN80_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1540879.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19760..20026)
                     /locus_tag="VYN80_000944"
     CDS             complement(19760..20026)
                     /locus_tag="VYN80_000944"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1540880.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20142..20585)
                     /locus_tag="VYN80_000945"
     CDS             complement(20142..20585)
                     /locus_tag="VYN80_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1540881.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            20689..21624
                     /locus_tag="VYN80_000946"
     CDS             20689..21624
                     /locus_tag="VYN80_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1540882.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21678..21962
                     /locus_tag="VYN80_000947"
     CDS             21678..21962
                     /locus_tag="VYN80_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1540883.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22019..22237)
                     /locus_tag="VYN80_000948"
     CDS             complement(22019..22237)
                     /locus_tag="VYN80_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540884.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(22234..22710)
                     /locus_tag="VYN80_000949"
                     /pseudo
     CDS             complement(22234..22710)
                     /locus_tag="VYN80_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6683 a         5106 c         4351 g         6570 t
ORIGIN      
        1 cttatttttc tatttaaatc gcttctgtaa caaagctacg actttcaagt accttaagca
       61 tggcattagc tgtatctagc gctgtgaaga ggggcacccc gtgttcaatg gctgaacgac
      121 gaatttgctc accatcttcg tcagcagttc gttttgttcc tactgtgtta atgatagctt
      181 gaattcttcc tttgcgtaca aaacttggga tatccttatc gtcatcacca atcttaccaa
      241 caggttgggc ttgcaagcca tgactagcaa agaaggctgc tgtcccttct gtcgcaagga
      301 ttccataacc aatgttttgg aaacgacgag ccaagttcaa ggcttcttct ttggcatcat
      361 cagcgatggt aaagacgaca ttaccaaaag ttggcaagtg tagataagaa gcttcaaagg
      421 ctttatagag agctttttcc aaagtagcat cagaacccat aacttcacct gttgacttca
      481 tttcaggacc gagcaagctg tctaccttag ctagtttggt aaaggagaag acaggtgcct
      541 tgatatgaac acgggtgctt tcagggtaaa gtccattttg gtagccaagt tctgataaac
      601 tttgaccaag aatgagcttg gtcgctactt gagccatagg aatattggtt accttagata
      661 ggaatggaac ggtacggctg gcacgtggat tgacctcaat aacgtagact ttttcatcct
      721 tgataacaaa ctggatgttc atcattccaa ggcagtgaag accgattgct aagcgtttgg
      781 tgtagtctgc gatggtctcc tgaacctttt gcgacaaggt ttgtggtggg taaacagcca
      841 ttgagtcacc tgagtggaca ccagcacgtt cgatatgctc catgatacca ggaatgagta
      901 catttttacc atctgaaatg gcatcaactt cgcactcttg cccaacgata taagagtcga
      961 caagaactgg atggtctgga ctagccttaa cagcagttcg catgtaagaa cgaaggtctt
     1021 cttcgttttc aacgatttcc atggcacgtc caccaagtac ataagatggg cggacaagaa
     1081 ctgggaagcc aatcttacga gctgcaagag ctgcttcttc ttcattggta gccgtttgtc
     1141 ctggtggctg tggaatatcc aattctttga gagcttgctc gaagaggtca cggtcttcgg
     1201 cacggtctag gtcagcaacc tgtgtaccaa ggatggtcac acctgctttt gccaatggct
     1261 ccgcaaggtt gatggctgtt tgaccaccaa actgaacgat aactcccttt ggttgttcca
     1321 agtcaatgac gttcataaca tcttcgaatg tcaatggctc aaagtaaagc ttatctgata
     1381 cagagaaatc tgttgaaacg gtctctgggt ttgagttcat gatgatagct tcataaccag
     1441 ctgcctggat agccttaaca gagtgaacgg ttgcgtagtc aaactcaacc ccttgaccga
     1501 tacggattgg acctgaacct aggacaagta cagattcttt atcagatctg atagattcat
     1561 tttcccaacc ataggttgaa tagaaatatg gcgtttcaga gtcgaactct gccgcacaag
     1621 tatctaccat cttataaact ggaacaatct tgttttccaa gcgaagttgg cgaacttgat
     1681 catcagtcgt tccccagagt tcagcaatct tacggtctga gaaaccatta agtttggctg
     1741 ttttcaaaac ttctagatct tgtggatgag cacccaattc ttgctcaatt tcaaagatat
     1801 gcaagagttt atcaagatag aagatatcaa tttttgttag ctctgcaatt tcttcaggtg
     1861 tgtagccacg acgaattgct tctgatacgt agaagagacg gtcatcttgg gctttgacaa
     1921 ccttttcaat caaggcatca tcagaaactg ctgcaagttc aggtatttca ttgtggtgca
     1981 ccccagtttc aagggagcgg caggccttga gaagagattc ctcgatatta cgaccgattg
     2041 ccatgacttc tccagtcgcc ttcatttgtg taccgagacg gcgttcaccc ttttcaaact
     2101 tgtcaaatgg gaaacgtgga atcttagcaa ctacgtagtc aagggctggt tcaaacatgg
     2161 cataggttga acctgtaact gggtttataa cctcatccaa ggtcaaacct actgcaatct
     2221 tggcagccaa cttagcaatc ggatatccag tcgctttaga agcaagggct gacgaacgtg
     2281 atacacgagg gtttacttcg ataacataat acttgaagct gttaggatca agagctagct
     2341 gaacattaca tccaccttca atcttgaggg cacgaataat gctcaagctc gcatcacgaa
     2401 gcatttggtt ttcatagtct gacatggttt gcgcaggggc aaatacaatg gaatcccccg
     2461 tgtgaatccc aactgggtca aagttttcca tgttacaaac aaccaaggca ttgtcagctg
     2521 agtcacgcat cacttcgtat tcaatttcct tgaaaccggc aatcgaacgc tcaatcaaac
     2581 attgggtaac aggtgacaat ttcaaaccat tttcagtgat ttcacgcaat tctttctcat
     2641 tggcacacat accaccacca gtaccaccga gggtaaaggc tggacgaacg atgactgggt
     2701 agccaattgt cgctgcaaag gcaactgctt cttctactgt gttaacaatt tcagattctg
     2761 gaatgggttg ttcaagctct tccatcaatt gtttaaagag gtcacggtcc tccgcttggt
     2821 caatggcaga taatttggta cccagaagtt caacgccaag ctcatctaag ataccatttt
     2881 tagacaattc catggccatg ttgagacctg tctgaccacc gagtgttggt agcaaggcat
     2941 ctggacgttc cttacgaaga atacgtgtca caaactcaag tgtaatcggt tcaatgtaaa
     3001 ccttgtcagc aatttccttg tccgtcatga tggttgcagg atttgagtta accaaaacaa
     3061 cctcataacc ttcctctttc aacgacaagc aagcctgggt cccagcatag tcaaactcag
     3121 cagcctgacc aataataatc ggaccagaac caatcaccat aattttttga atatcagtac
     3181 gtttaggcat agtttatgat acaaagcccg taaagaacac agtgaaaata ggaaactcgg
     3241 cgcagacgcc ttcagcgtca agacgatgtt tatctttttc acacagttct tagggcgtgt
     3301 tcacttccgc gaacaatgta tcttctcctt tctattcgtc gcctctcagg gcgacattaa
     3361 ataagataca aaggacgaat agaaagcgat tgaattttag aaaatcaagg aaggattgac
     3421 aatccaagtt ggtttctcta cattctgagc tttccgtccg tgttcagtta cataaattct
     3481 ccgacgagct tttactcgtt cttagtttga ttgtttaaaa acttccatca tctcgataaa
     3541 ctcgtcaaat aggtagctag cgtcgtgtgg cccaggagct gcatctgggt ggtattgaac
     3601 agagaaagca ggttggtatc tgtggcgcac accttccact gacttgtcat tgatttcttc
     3661 gtgggtaata atcaagtgct ctggcaaatc ctcgcggctg actgcataac catggttctg
     3721 gctggtgaag tctactcgtc ctgttgcgat ttcacgtacc gcatggttga atccacggtg
     3781 gccaaacttc atcttatagg tcttagcccc gtttgccatt gcaaagagtt ggtgtcccat
     3841 acaaatacca aagattggaa tttttccttg tacaccgcga atcatgtcga gtgcttgtgg
     3901 aacgtcttct gggttacctg gaccatttga caacataact ccatcaggat tgagatggag
     3961 aatttcttca gccgttgtcg aataaggaac aactgtcacg ttacagttgc gtttagaaag
     4021 ttcacgtagg attgagtgct tgagaccaaa gtccactagc accacgctca aaccaactcc
     4081 tggagctgga taagacgttt tagtagaaac ctgtttgata ttgtctgtcg gtaaaactgt
     4141 tgcttggagc tggtccgtca catggtccat actgtcccca acatgggtca aggttgcacg
     4201 catagtacca tgcttacgga taatcttggt aagagcacgc gtatcaattc ctgaaatccc
     4261 tggaattttc ttggctttca aaaattcatc caaggtcatt tggttgcgcc agttgctagc
     4321 tctacgcgct tcttcaaaaa caacgactcc cttacaagtt ggaataatgg attcataatc
     4381 atcacgatta ataccataat ttcctaccaa aggataagta aaggtcaaga tttgtccatt
     4441 ataagactgg tctgtaatgg attcttggta gccggtcatc cctgtattaa agacgatttc
     4501 gcctgttaca tcaatatctg ctccgaaggc cttgccttca aaaactgtgc catcttctaa
     4561 tactagaatt ctttttgtca tattttcacc tctcgtggac gctcactggc gtcttttaac
     4621 gtcttgtgtt ttagttggcg tttctactcg ctagtacgga ttctaagatt gccattcgaa
     4681 caaagacacc attggtcatt tgttggacaa tccgtgattt tggtgcttca accaagtggt
     4741 ctgctatttc tacatcacga ttgattggag ctgggtgcat gaggattgct gtttctttca
     4801 aacgatcgta acgttcttga gtcaagccat gttgggcatg atagtcttct tttgaaaata
     4861 cagctccact atcatggcgt tcgtgttgca cacggagaaa catcatgaca tcaacctgat
     4921 caatgatttc atcaatggtt acaaactttc catagtctgc aaactcttga cttctccatt
     4981 cctcaggtcc agcaaaaaag agttcagctc ccaagcgttt caaaatctgc atattggatt
     5041 tggcaacgcg tgagtggtcc aagtcacctg caatagcaac tttaagaccc tcaaagtggc
     5101 caaattcctc ataaatggtc atcaaatcaa gcaagctctg gctagggtgt tggcccgaac
     5161 catctccacc attgatgatg gaagtcgtaa tcgttggact agcaatcaat tctctatagt
     5221 agtcgacctc tggatggcga atcacacaga catccactcc taaagcagac agagtcaaaa
     5281 tggtgtcata aagtgtctca cccttattaa ccgagctagt cttcacatca aagtcaagtc
     5341 gttccaatcc aagtttaatc tctgcgactt caaaggactt atgtgtccgt gtagaatcct
     5401 caaagaagag attggaaaca atcggatggt cttcataggg aagctgggct ccatttttaa
     5461 actcaattcc tcgcttgatc aatttcatta cttgatcgac agtgaggtct tccatggaca
     5521 ccacatggtt caatgcttgt tgattttctg acatggctac tcctttaact ttctaagctt
     5581 cttcagtaat cagaactctg tcttggtcat caagttctgt catctctacg atgatttctt
     5641 cagaacgact ggttgggata ttttttccaa cgtaatctgg acggattggc aattctctat
     5701 gtccacgatc gactagaact gctaaactca cacgcgcagg acgaccatga ccgacaatat
     5761 tatcaatagc agcacggatg gtacgacctg tatagagcac atcatccacc aagataactt
     5821 cgcggtctgt cacatcgaca gaaaccaaag aagtatcttc tccactttta acatcatcac
     5881 ggaaaggttt agtatccaat tccacaacag gaactgaaag attttctaac tgcttcaaac
     5941 gttcttggat tcggtgggca ataaagacac cacgagtttt aataccagcc aagacaatct
     6001 tattcaaatc tttgttgcgt tcgataatct cataagtaat acgcgtaatc gctcgtttga
     6061 cggtcaattc gtctacaact tcttttgttt tcatgacaaa cctccaaaaa gaaaagtctc
     6121 cttaaacaag gagacttgaa atttatagcc aagcgagccc tactgcacac agtatagact
     6181 tcacccttct actttatcgc gctccttgcc tgcctcacgg gacaggttta aaggaatatt
     6241 tagttatcat ttactatagc acaaagcatg cttaaaatca agcaaaaagt ttcaatgtag
     6301 catcttacaa attgctaaaa tcatataatt gtgggtactg gtcacactct ggattttttg
     6361 gatggcaaat ggctcttcca aaataaatca tggcctgatg ggcagctaac cactgctcag
     6421 gcggcaagat atccatgacc cgcttttcca cctcaagtgg cgtcgctgat tttttgacaa
     6481 tatcgtggtg tttgcaaata cgctccacat gagtatccac tgcaaaggct ggaatcccaa
     6541 atcctacact catgacaaca ttggctgtct tgcgaccaac acctgccaaa ctctccaatt
     6601 cttcacgtgt ctgagggact tgaccatcaa aatcgtctag taactgttgg gcacattttt
     6661 taaggaattt agctttattc cgatacaatc ccaagcgaga aatatgtgaa gcaatctcac
     6721 tctctgtcgc tacagacata gcttggggtg ttggaaaggc aacaaagaga cctggtgtgg
     6781 ccttatttac cgctgcatct gtcgtctggg ctgataacat gaccgcaacc aggagttcaa
     6841 aatgattggt aaaatcaaga ctaggcttgg catctgggaa gagggcaatg atttcttcta
     6901 gcacctttcg tgctcgtttt tttgacaaga ccattattca tctccgtcaa atagtccttg
     6961 taagccagca aaaggactgt tttcttcttt ctttactgct tgttgagctt ggtattcttc
     7021 ctctgtcatg atttgccagt catttcctga gacaaatcct tgaccagctt cttcttcagc
     7081 cgtcaagacc ttgataggaa tgtttagcag gatattgtct gatacactct cagcaaggtc
     7141 aagctcccca ttttcgatgg gcaagaccaa gtcatcatct aaaacttctt gatctagctg
     7201 gttagttgcg ccttccatga aaacttccgt gactggataa gattcaacta actcaactgg
     7261 ctccatactg cgactcgaag caagaacaat ggtataagat agttgataat ctaagaaata
     7321 catacggtct tcatattgta ctttcccaac tgcaaggata tcttttacat ctaaaatttc
     7381 ttgattacgt gcacgcaggt catcaactaa atctaacgtt tgttcaaagt tcaaaccttc
     7441 agactgctta cgaatttctt gaatatttaa tttcatactt cctccataaa gatttactct
     7501 cttgattata ccatgaaaag gctacaaatc agcacaccaa actttgtaat taaaattcga
     7561 aattttaaca tattcactat gacagtttta ttttttagtg ctatactata gggaaagagt
     7621 acatcagatc aagaaggatg ctcacacgga agacaagaaa ctcattcaac tcctatccaa
     7681 gttaaataaa agctaccaaa actgtaaaca gggtacggca gatgatattc gactacaaga
     7741 gctgctaaac actactatgc aagaactcaa aaaacggaaa agttgaacaa cagtatctta
     7801 attgatcttg agaaatttta ccaacccacc agtcctatga ttggactggg tagcctaaaa
     7861 ctaaacaatc aagaacgcac tgcttggcga aactatgata aaatccatta cgagcatgtc
     7921 aaacacgtac taagtctcta tagacctgtt tttggatttt agagcataga atttccagtt
     7981 ttctgttgac aaaatttcct taaaagtata gtataaagat actaatacta ggaggtaagg
     8041 gagacatgaa caactaagtc tatcaaataa agaaccttta tttagtagat cttgtttttg
     8101 tctctttttg tgtgctcttt tatgcccttt ttctggcatg ttaatagagt ttttttgaca
     8161 tagactttgg gctctactag gtaaagtaga gctttttgtt atgcactatg aacattctag
     8221 aaagggcaat agtatgataa aaatcaatca cctgaccatc acacaaaaca aagatttacg
     8281 agaccttgtc tctgacctaa gcatgaccat acaagacggg gaaaaggtag ctattattgg
     8341 cgaagaagga aatggtaaat caaccttact taaaacttta atgggggaag atttgtctga
     8401 tttcactatc aagggagaca tccagtctga ttatcagtca ctggcctaca ttcctcaaaa
     8461 acttcccgag gagctgaaaa agaaaacttt acacgactac ttctttttag attctattga
     8521 tttagactac agtatcctct atcgtttggc ggaggaattg cattttgata gcaatcgttt
     8581 cgcaagtgac caagagattg gcaatctatc agggggagaa gctttgaaaa ttcagcttat
     8641 ccatgagtta gccaaaccct ttgagattct atttttagat gaaccttcaa atgacctaga
     8701 ccttgagaca gttgattggt taaaaggcca gattcaaaag accaggcaaa ccgttatttt
     8761 catttcccat gatgaagact ttctttctga agcggcagac actattgttc acttgcgact
     8821 ggtcaaacac cgtaaagaag cggaaacgct agtagagcat ttagactatg atagctatag
     8881 tgagcagaga aaggctaatt ttgccaaaca aagtcagcaa gctgctaaca accaaagagc
     8941 ctacgataaa accatggaaa aacatcggag agttaaacaa aatgtagaaa ctgcgcttcg
     9001 agctaccaaa gatagtactg ccggtcgcct attggctaaa aagatgaaaa ctgtcctctc
     9061 acaagaaaaa cgctacgaaa aagcagctca gtccatgact caaaagccac ttgaagagga
     9121 acaaatccaa cttttctttt cagacatcca accattacca gcttctaaag tcttagtcca
     9181 actggaaaaa gaaaatttgt ccattgacga ccgagttttg gttcaaaaac tacaactaac
     9241 tgtccgtggg caagaaaaaa tcggtattat cgggccaaat ggtgttggga aatcaactct
     9301 gttagccaaa ttaaagcaac ttctgaatga taaaagagag atttcccttg gttttatgcc
     9361 acaagattac cacaaaaaac tgcaattgga tttatcacca atagcttata tcagcaaaac
     9421 tggggaaaaa gaggaacttc agaaaatcca atctcaccta gccagtctca atttcagtta
     9481 tccagaaatg cagcatcaaa ttcgctcctt atctggcgga caacagggaa aactcctgct
     9541 tttggatcta gtcctgcgca aaccaaactt tctcctgctg gatgaaccca cacgaaactt
     9601 ttctcccact tctcaacccc aaatcagaaa actctttgca acctatccag gcggtctcat
     9661 cactgtttcg catgaccgtc gtttcttaaa agaagtctgc tcgatcatct atcgcatgac
     9721 agaacacggt ttgaagctag ttaatttaga agatttataa atttgcaaca tagcaaaaat
     9781 ccagagacga cctctggatt cttttacatc tgttttaaac gttcaatccg ttctgagata
     9841 ggtgggtggg tataaaagag tttttggaac cccccacctt tcttgggatc attgatataa
     9901 agggcactgc tagcatcatc gacgtggcga ctcataggtt tgctattgtc caacttatct
     9961 agggcattaa tcattccctg gggattgcga gtcagctcga cactagatgc atctgccaga
    10021 aattccctct gacgagaaat agcgagctga accaaggttg cagcgagagg tgccagtaca
    10081 atagctagta gggaaaccac tagcataatg atttcaagac catttccatc tcggtcatca
    10141 tcacttcgtc tgcgacctgc tccaccccac cacatcatac gacctgccat actagaaagc
    10201 atggtgatag cactagcaag ggcaactgca atagtcgaaa tacggatatc ataattacga
    10261 atatgactga cttcatgtcc cataacagct tctagttctt cacgattcat gatagctagt
    10321 agacctgaag tcgcagcaac agccgcattt tgaggattag aacctgtcgc aaaggcattt
    10381 aaggctggat catcaatgat gaaaacacgg ggcataggaa tctgagcgac cagagccata
    10441 tcttccacta catggtagag gtctggtgcc gtttgctcat ccacctcacg cgctccattc
    10501 atggacatga caatttctgt cgattgaaaa atcatagaca aagcgtagat aaagccgata
    10561 atcagtgcaa taaccaaacc accaagtcca gatcttataa agagataacc aaccgcataa
    10621 ccaacaagag ctaagagtag gaaaaatacc agcaacaaaa tccaggtttt tcgtttattg
    10681 cttgcaattt gatcaaacaa catcttagtc acctaaaccg ctaaaatcaa ctttaggaac
    10741 cgacttttcc tcttcaggtg tttgaaggaa atctgccgct ttaaatccaa acattccagc
    10801 gataatattg ctcgggaaag tttctaattt tacattgtag ttgctgacaa cactgttata
    10861 gagttgacga gagtaagaaa ttttattttc tgtgtttgtc aactcctctt gcaatttaac
    10921 aaagttagca ctagctttca aatctggata gctttctgca actgcaaaaa tacctgaaac
    10981 ctgacgagtg agggcatcac tggctttcat agcttctgct ggtgaagtcg ctgccgccac
    11041 ttggttacgt agttctgcca ccttttcaag ggtagaacct tcatatttgg cataaccttt
    11101 tacagtctca atcaagtttg gcaagaggtc atttcgacgt ttcaactgaa catcaatctg
    11161 actccaagcc tccttggttt gcatacgatt tttaaccaaa ccgttatagc taacaatcac
    11221 aaaaataaca ataagagcga taactccaag aataatccaa gtcataatat aagtcctttc
    11281 tgcttttaga ttagtaccag tatatcaaat tttctatgat tgtggtaaaa taagatgata
    11341 cttaaagaag gaaataacta tgaaaccaaa aacattttac aacttgcttg ccgagcagaa
    11401 tcttccactt tcggaccagc aaaaagaaca atttgaacgt tattttgagc tcttggtcga
    11461 gtggaatgag aagattaatt tgacggcgat tacggacaag gaagaagttt atctcaaaca
    11521 tttttacgat tcgattgcac ccattcttca aggtttgatt cccaatgaaa ctatcaaact
    11581 tcttgatatc ggggctgggg caggatttcc tagtctacca atgaaaattc tctatccgga
    11641 gttagatgtg accattattg attcactcaa taagcgcatc aacttcctac aactcttggc
    11701 tcaagaactg gatttgaacg gagttcattt ataccacgga cgtgccgaag attttgccca
    11761 agacaagaac ttccgtgctc aatatgattt tgtaacagct cgtgcggttg cccgtatgca
    11821 ggtcctatct gaattgacca ttccctacct taaggttggt ggaaaactat tggcactcaa
    11881 ggccagcaat gcgcctgagg aattattaga agctaagaat gccctcaatc tcctttttag
    11941 taaggtcgaa gacaatctca gctacgccct accaaataga gatccgcgtt acatcacagt
    12001 ggtagaaaag aaaaaagaaa caccaaataa atatccgcgt aaggctggta tgccaaataa
    12061 acgcccactt taaatttttt agtaaacaaa tgtttacaaa atcagcctcg ctcttttatt
    12121 tctaggctcg ggaaaaaatg atttacaaaa tcagcctcgc tcttttattt ctaggctcgg
    12181 gaaaaaatga tttacaaaat catttttttc tgctatacta tcctaagcaa aggtttttaa
    12241 tgtcatcccg tgaggtgacg aagacgcaaa aatatttgaa actctttaaa gtctagattt
    12301 tagagaagtc ttactctgag ggcctattgc tgtaaaataa tgggctcttt tctgatgccc
    12361 aaaagtgagg tttatatgaa acaagaatca actgttgatt tgttattaga cgttgaccaa
    12421 cgtccttcgg ctggaaaagg aattctcctt agtttccaac acgttttcgc catgtttggt
    12481 gcgaccatct tggtaccatt gattttggga atgcctgtat ctgttgccct ttttgcttca
    12541 ggtgttggaa cactcatcta catgattgct actggtttta aagttccagt ttatctaggt
    12601 tcttcattcg cctttatcac agctatgtca ctggctatga aagaaatggg gggggatgta
    12661 tctgctgctc aaacaggggt tatcttgact ggtttggtct atgtccttgt tgctaccagc
    12721 atccgatttg taggaacaaa atggattgat aaactcttgc caccaatcat tatcggtcct
    12781 atgatcatcg ttatcggtct tggacttgca ggttcagctg ttaccaatgc aggtcttgta
    12841 gcagacggaa attggaaaaa tgctctggta gccgttgtta ctttcctaat tgctgccttt
    12901 atcaatacaa aaggaaaagg cttcctacga atcattccat tcctctttgc cattatcggt
    12961 ggttacctct tcgcactaac tcttggtttg gttgacttta cgcctgtcct agaagctaac
    13021 tggtttgaaa ttcctggttt ctacttgcca tttagcacag gtggtgcctt taaagagtac
    13081 aatctttact ttggtccaga agccatcgct atcttgccaa tcgctatcgt aacaatttct
    13141 gaacatatcg gagaccatac tgttttgggt caaatctgtg gccgtcaatt cttaaaagaa
    13201 ccaggtcttc accgtactct tcttggtgac ggtatcgcaa cttctgtttc tgccttcctt
    13261 ggtggaccag ccaatacaac ttacggagaa aatacagggg ttatcggtat gactcgtatc
    13321 gcttctgtct cagttatccg taacgctgcc ttcatcgcga ttgccctcag cttccttggt
    13381 aaattcactg ccttgatttc aactattcca aacgctgtac ttggtggtat gtcaatcctt
    13441 ctctatgggg ttatcgccag caatggtttg aaagtcttga ttaaagaacg tgttgatttc
    13501 gctcaaatgc gaaacctcat catcgcaagt gctatgttgg ttcttggact tggaggagct
    13561 atccttaaac ttggtccagt tacactttca ggtactgccc tttcagccat gacaggaatc
    13621 atcttgaact tgatcttgcc atacgaaaat aaagactaag agtctaaata cacctaatcc
    13681 actcagacag ctgagtggat ttttcgcata ccataataaa agtgtcttaa caaaattatt
    13741 aaaatcaaaa aacgtataat atcagatatt ctaaaacctt gatactgtac gttttatcat
    13801 agaaattttt actttatttt ctcatcaaat aagatttgca tcaatctctt gtcttacttg
    13861 cgtttcttct tcgctttctt cattttgtta gccatacgtt tcatggactg tttcatggca
    13921 aattcaccaa ttttaccttt caaaccgcca ccaaacatct ggctcatatc tggcattcct
    13981 gctcctccga gagctgataa gtcagacata ccgccttgtc ccatcattcc ttcaagggca
    14041 gacatatcca ttcctcccat atttggcata tttttaggaa ggttatttgg attaatcccc
    14101 atttgcttca tcattttatt catatcccca gacataacac cctgcatgag ctgtttagcc
    14161 tggttaaagt ccttgatgaa tttattgact tcgacgaatg tatttccaga accagcagca
    14221 atacgacggc gacggcttgg atttaacaaa tctgggtttt cacgttcttc aggtgtcatc
    14281 gaagacacaa tggcacgttt acgagcaatc tggcgttcat ccaccttcat gttttgaagt
    14341 gctggattgt tggccatacc tggaatcatt ttgagcaagt cttccattgg ccccatgttt
    14401 tgtacctgat ccaactgatc aatgaaatca ttgaaatcaa aggtattttc acgcatcttc
    14461 tcagccattt caagggcttt ttgctcatcg tattcttgag aagctttctc aatcaaagta
    14521 agcatatccc ccataccaag gatacggcta gacatgcggt ctgggtggaa ggtttcaatg
    14581 tccgtaatct tttcacctgt accagtgaac ttgattggtt ttccagtaat gtgacgaaca
    14641 gacagagcag caccaccacg agtatcacca tcaatcttgg taaggatgac cccagtcact
    14701 tccaactgag cattaaactc acgcgcaaca ttggctgctt cctgaccaat catagcatca
    14761 acgacaagca agatttcatt tggttgagcc aatgctttca catcacgaag ctcattcatg
    14821 aggagctcat caatctgcaa acgacccgca gtatcaatca agacatagtc gttatgatta
    14881 gtttgggctt gctccaaacc ttgacgtaca atctcaacag ctggtacttc tgttccaagt
    14941 gcaaagacag gcacatcaat ctgttgtccc aaggtcttaa gctggtcaat ggcagctgga
    15001 cgataaatat ccgccgcaat catcaaagga cgagcatttt cttctttctt gagtttgttg
    15061 gccaatttac cagcaaaggt tgttttacca gccccttgta aaccaaccat catgatgatg
    15121 gttggaatct taggtgactt gataatttct gccgtatcag aacctaaaac ggctgtcagt
    15181 tcctcatcaa cgattttaat aatctgttgc gcaggattaa gtgtatcaat gacctcatgc
    15241 ccgactgcac gctcacgaac tttcttgata aagtccttta caacaggcaa ggcaacgtcg
    15301 gcctcgagca aggccaagcg aatttctttg gttgcctctt ggacatcaga ttcagagatt
    15361 tttccttttt tacgtagatt tttaaagacg ttctgcaaac gttctgttaa actttcaaat
    15421 gccatttttc ttcctcttat tctctattat caatgcttgt taaaatttct atctgctcct
    15481 gcagaaagtc atccttggga tagcgctcca aaatctgatc aaaaatctga ctgcggacaa
    15541 tatagtccga gtacatgtgc aatttcatct cataatcttc cagaatcttt tctgttcgct
    15601 tgatattgtc atagacagcc tgacgactga caccgaactc ctcggcaatt tcagcaaggc
    15661 tgtaatcatc agcgtagtag agctcgatat aattcatttg cttatctgtc aaaagcgccg
    15721 cataaaattc aaagagcgca ttcatacgat tggttttttc gatttccata acttttatta
    15781 taccaaaaat tagcctaatc taccacacta ggaagccaat ccaagaagat agatagctaa
    15841 atttgaaaaa gacatgagcc tagccccaag taatttccaa ttgatagctg gcaaagggat
    15901 gtccctcttg attttgtagt tgataatcta gttcaatctt ttgcctatca acttgataat
    15961 ggctcgtttg gatggtaaac tcctgcatgc ccataggtgt aagaatatag gctaaactat
    16021 cgctatcctt tagaaagcgc ataatggtct tgggattaga aaatcggctc atcacaagtt
    16081 cttgaccatg aaatttaatc actacttttt ccttttcctc attatagaaa agcaggtagc
    16141 tataatctct tttttcatgc acttccacat cataaagctg gtcaatcact tccaactgct
    16201 catcaaactg aatcgtattt cgcatccgaa tcttcacatc aggtcctctt tcttgtctct
    16261 tgtcctacta ttttaccaaa aagagcagga ttttgctata atggtcatat gaacgaaaaa
    16321 gtattccgtg accctgttca caactacatc catgtcaata atcaaatcat ctatgacttg
    16381 attaatacaa aagaatttca acgtttgcgc cggatcaagc aactagggac ttccagttat
    16441 accttccacg gtggagagca tagtcgcttc tctcactgtc tgggagtcta tgagattgca
    16501 cgacgtatca cagaaatttt tgaagaaaaa tatcctgagg aatggaatcc tgccgagtct
    16561 ctcttgacca tgaccgctgc tctcctacat gaccttgggc atggcgccta ctcccatact
    16621 tttgaacatc tctttgatac agaccatgaa gccattactc aggagattat tcaaaatcct
    16681 gagacagaga ttcaccaagt cttgctacaa gtagcgcccg atttcccaga aaaggtggcc
    16741 agtgtcattg accataccta tcctaataag caggtcgtgc agctcatttc tagtcagatt
    16801 gacgcagatc gcatggacta tctcttgcgc gactcctatt ttacaggagc atcctatggg
    16861 gaatttgacc tgactcgcat cctccgagtc attcgtccta tcgaaaatgg tatcgccttt
    16921 cagcgcaatg gcatgcacgc catcgaagac tacgtcctca gtcgctacca gatgtacatg
    16981 caggtttatt tccaccccgc aacacgcgcc atggaagttc tcctgcagaa tctcctcaaa
    17041 cgcgccaagg aactctatcc tgaagacaag gacttctttg cacgaacttc tccacacctc
    17101 ctgcctttct ttgaaaaaaa tgtgaccttg actgactatc tggctctaga tgatggtgtg
    17161 atgaatacct actttcaact ctggatgacc agtcctgaca agattctcgc agacttgtca
    17221 caacgctttg tcaaccgcaa ggtctttaaa tccattacct tttcacaaga ggaccaagat
    17281 caacttacta gcatgagaaa attggttgag gacatcggct ttgatcccga ctactatact
    17341 gccattcata agaactttga cctcccttat gatatctatc gtcccgaatc tgaaaatcca
    17401 aggacacaga ttgagatttt acaaaaaaat ggagaactgg ccgaactctc tagtctgtct
    17461 cctatcgtcc aatcccttgc tggcagtcgc cacggagata atcgtttcta tttccctaaa
    17521 gaaatgttag accaaaacag catcttcgca agcattaccc agcaattttt acacttgatt
    17581 gagaacgatc attttacccc aaataaaaac tagaagagga attttatgag tattaaacta
    17641 attgccgtcg atattgacgg aaccctagtc aacagccaaa aggaaatcac tcctgaagtc
    17701 ttttctgcca tccaagatgc caaagaagct ggtgtcaaag tcgtgattgc aactggccgc
    17761 cccatcgcag gtgttgccaa acttctggac gacttgcagt tgagagacca aggtgactat
    17821 gtggtgacct tcaacggtgc ccttgtccaa gaaactgcta ctggccatga gattatcagc
    17881 gaatccttga cctatgagga ttatctggat atggaatttc tcagtcgtaa gctcggtatc
    17941 cacatgcacg ctattaccaa ggatggtatc tacacagcta atcgcaatat cggaaaatac
    18001 acagttcacg aatcaaccct cgtcagcatg cctatctttt accgcactcc tgaagaaatg
    18061 gctggcaagg aaatcgtcaa atgtatgttt attgatgaac cagagattct cgatgctgca
    18121 attgaaaaga taccagcaga attttacgaa cgctactcca tcaacaaatc tgctcctttc
    18181 tacctcgaac tccttaaaaa gaatgttgac aagggttcag ccattactca cctagctgaa
    18241 aaactaggat tgaccaaaga tgaaaccatg gcaatcggag acgaagaaaa tgaccgtgcc
    18301 atgctggaag tcgttggcaa ccctgtagtc atggaaaacg gaaatccaga aatcaaaaaa
    18361 atcgccaaat acatcaccaa aacaaatgac gaatccggcg ttgcccatgc tattcgtaca
    18421 ttggtactgt aaaaggaaag aatagataaa aaccgctcag ctgagcggtt tttgatgtct
    18481 aattactgcc ccctgcagga atcgaacctg caactactcc ttaggaggga gttgttatat
    18541 ccattgaact aagggagcta gataaaaact ctgctgaaaa gcagagtttt ttagtcgaat
    18601 taacgacgga tttctttgat acgagctgct ttaccttgaa gagcacgcaa gtagtacaat
    18661 ttcgcacgac gtactttacc gtaacgaaca acttcgattt tttcaacacg tggagtgtgg
    18721 attgggaaga tacgctcaac acctacaccg ttagagattt tacgaactgt gtagttttct
    18781 gagattccag cacctttacg tgcgataaca acaccttcaa aaatctggat acgttcacgg
    18841 ttaccttcga caactttcgc gtgtacacga acagtatcac caggacggaa tgatgggata
    18901 tctgtacgaa gttgaccttc agtcaagctt tggattaatg gattcatttt attctcctat
    18961 ctttgtcaat cttgaggaat cttcctcagc ggataaactg tatttttgtg cgtccattac
    19021 acacaagata tagtttacca aatttctaaa aaaaagtaaa gaaatttata gcaaaattcc
    19081 taaaaaaatc gcaaccaaac cacctaggta ggtcaaaatc aggtaagaat aaaatacctt
    19141 cttatcgctt agcaaccttt gaagttcgtc attcaaggtc gaaaaagttg ttaaaccgcc
    19201 acaaaatcct gttgctagaa tagcatatac ttccttagac tctacatgat tgtagaatac
    19261 tccaattaaa aaacaaccta aaagattagc tatgagcgtt ccgagtggca atttagaagc
    19321 ttgattatag cgagaaaaga aataccgtac gagtgctccg aaaccacaag caattgcaag
    19381 atagacgatt accatttctt cctccctaga aaataagcta agagtaggcc tccaccgatg
    19441 ctcaaaagca aatacaggac taaactaaaa taacgccctg tatcaagcag cttcacagta
    19501 tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc cagtgctaaa
    19561 atcaagccct tactagtccc cttatagacc agatagccct tgacaagaaa aatcaagcag
    19621 aaaattccca gatagttgac aaagagagtg ccccaaggaa agtctggact ggctggtaac
    19681 caggtggaaa ctagatatcg gacaagtccg cccaacatag cagctagaaa aattcctagc
    19741 ggataaaatt gttctttttt catttgatat tttgatcctg ataatcacgc gaacgtttga
    19801 gtatgtccga aaaagtcgct acaatagtct cctgatagcg cttgtcctct acacgactcc
    19861 taaccttttc aaaaataact tcttctctct tagtatctaa aatcggttta cctgaagctt
    19921 tcttataagc aaccaccccc tcaactaaat gcattcgttc ttctaacagt ttgacaattt
    19981 ggtcgtcgat ttgatcgatt tcttgacgaa taatatctaa atccatacag tctcctcctt
    20041 tatttgaatt attgtatcaa aaagccatca attaggctag taaaatccca actctcgata
    20101 agaaaaatgc actgattgat tacatcagcg cacttttatg tttatcctac tttagcagcc
    20161 aattcttctg cgaattgttc caagcgttca atatcttctt cctcggcaga aagatcaact
    20221 ttaacacact ctgaaccttt ttctgctcct gttgacacaa agacacggtc aaagtcatca
    20281 acagccttac agaattcatc gtagaaggta tctcctgagc cgaccactcc gtagattttg
    20341 ccattcaagt tgagatctgc taggtcttcg tagaagtcca tcatctcatc tggcaattca
    20401 ccatcaccat aagtataggt cgcaacgata gcgatgtctg cttccaagaa gtctgaagcg
    20461 tcaacagttg tacattcatc aacatcaaca tccaagccca agtcacgtaa tttgtctgct
    20521 acaatatctg caatttcttc ggtattaccg gtcatactgg caaatacaat ttttgctaat
    20581 gccatatcgt cctcctcaat ttatctttct ccattatatc acatcttttt cattttaaaa
    20641 ataaaaattc ttgtgctaca atgaaatgag aaagaattga ggttatttat ggagatttgc
    20701 caacaaattt tagaaaaaat taaagaatac gacaccatta tcattcatcg tcatatgaaa
    20761 ccagaccctg atgccttggg aagtcaggtg ggattgaaag ccttgctgga acatcatttc
    20821 ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg gatggctgag
    20881 atggatcttg ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg tgatacagct
    20941 aatactgctc gtatcgatga taagcgctat agtcaaggtg attttctcat taagattgac
    21001 caccatccaa atgatgatgt atacggtgac ctgtcttggg tcgatactag ttcaagtagc
    21061 gctagcgaga tgattaccct atttgcccaa acaacccaac tagccttggc agatcgcgat
    21121 gctgagttgc tctttgcagg aattgtcggt gatacaggtc gcttcctcta cccttctacc
    21181 actgcacgga ctcttcgcct ggctgcttat ttgagagaac ataactttga ctttgcggct
    21241 ctcactcgca aaatggacac tatgagctac aaaattgcta aactgcaagg ctacatctac
    21301 gaccatctgg aagtggatga aaatggtgct gctcgcgtta tcctgagtca agaagtcttg
    21361 aaacaataca atgtaaccga tgctgaaact gcagccattg tcggtgcacc tggacgcatt
    21421 gacagagtga gtctctgggg aatttttgtc gaacaggctg atggccacta ccgagttcgc
    21481 ttacgcagta aaatccatcc tatcaatgaa atcgccaagg agcatgatgg tggaggccac
    21541 cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa acgaaatcat ctaccaaaag
    21601 ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa tctgatagac
    21661 tagtatggta acaatctatg gctcgcaaag agaccatggc agaaaggaaa tattgcaaaa
    21721 tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt catggacaca actactggtt
    21781 accaattcct tagcggttca acaaaacgct ctaacgaaac agttgagttc gaaggcgaaa
    21841 cttacccatt gatccgtgtg gaaatttcat cagactcaca cccattctac actggacgtc
    21901 aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac ggtctcaaat
    21961 aatgataaga gaacagtttc ggctgttctt ttttgtttct tgaaatcaac tgctgttttc
    22021 atgttccaga ctcatctgta ggttcggttt ccatgctact aggcagaaag gaaatagctg
    22081 tttcaacacg tccataatga gctatactat tgtaacgaac cacactttca ttgatggtcc
    22141 aagtggaatt cattttctta aaagcttctc ggactttttc caaatctttg gaggcaatgg
    22201 cctgctctaa ggtttcaaaa cgaggactta tactcatctg ctttcacaaa gcattctagt
    22261 ccatctccga ttaacgatgg actttatcac ctccttctcc agtccttgta tgacatcttg
    22321 aagttgattc atgatatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg
    22381 aatctctttc cacacttgtt caatgggttc atctctggtg tgtatggagg aataaaggta
    22441 aaatcaatat tagtcggaat atttaaggta cttgatttat gccatatagc attgtccata
    22501 acgagtaaaa ggataagctt gtgaaagctc ttctaaaaag gcgttcatcc acactccttt
    22561 ttataagcct gaaataaggc atcaattgta acaaattctc ctgcctctgc agccttcaaa
    22621 tggcgggcaa gaaagacttt ctcttcctca actgtcatat atgcatggtt acgaccacca
    22681 cgtgtttctt gaaggagaga gtcgagtccg
//
LOCUS       DAVLWA010000025        21551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLWA010000025 DAVLWA010000000
VERSION     DAVLWA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.025"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            59..646
                     /locus_tag="VYN80_000950"
     CDS             59..646
                     /locus_tag="VYN80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1540885.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            573..2216
                     /locus_tag="VYN80_000951"
     CDS             573..2216
                     /locus_tag="VYN80_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1540886.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2227..3588
                     /locus_tag="VYN80_000952"
     CDS             2227..3588
                     /locus_tag="VYN80_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1540887.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            3645..3773
                     /gene="blpC"
                     /locus_tag="VYN80_000953"
     CDS             3645..3773
                     /gene="blpC"
                     /locus_tag="VYN80_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1540888.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(3844..5184)
                     /locus_tag="VYN80_000954"
     CDS             complement(3844..5184)
                     /locus_tag="VYN80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1540889.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5198..5935)
                     /locus_tag="VYN80_000955"
                     /pseudo
     CDS             complement(5198..5935)
                     /locus_tag="VYN80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5940..6278)
                     /locus_tag="VYN80_000956"
     CDS             complement(5940..6278)
                     /locus_tag="VYN80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540890.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6554..6883
                     /locus_tag="VYN80_000957"
     CDS             6554..6883
                     /locus_tag="VYN80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1540891.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7051..8100)
                     /locus_tag="VYN80_000958"
     CDS             complement(7051..8100)
                     /locus_tag="VYN80_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1540892.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFPETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8097..8828)
                     /locus_tag="VYN80_000959"
     CDS             complement(8097..8828)
                     /locus_tag="VYN80_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1540893.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            8896..9306
                     /locus_tag="VYN80_000960"
     CDS             8896..9306
                     /locus_tag="VYN80_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1540894.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9316..9603
                     /locus_tag="VYN80_000961"
     CDS             9316..9603
                     /locus_tag="VYN80_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540895.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            9765..9959
                     /locus_tag="VYN80_000962"
     CDS             9765..9959
                     /locus_tag="VYN80_000962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540896.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10293..11429)
                     /gene="dnaJ"
                     /locus_tag="VYN80_000963"
     CDS             complement(10293..11429)
                     /gene="dnaJ"
                     /locus_tag="VYN80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1540897.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(11831..12187)
                     /locus_tag="VYN80_000964"
     CDS             complement(11831..12187)
                     /locus_tag="VYN80_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1540898.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12189..14012)
                     /gene="dnaK"
                     /locus_tag="VYN80_000965"
     CDS             complement(12189..14012)
                     /gene="dnaK"
                     /locus_tag="VYN80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1540899.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14492..15016)
                     /gene="grpE"
                     /locus_tag="VYN80_000966"
     CDS             complement(14492..15016)
                     /gene="grpE"
                     /locus_tag="VYN80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1540900.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15043..16077)
                     /gene="hrcA"
                     /locus_tag="VYN80_000967"
     CDS             complement(15043..16077)
                     /gene="hrcA"
                     /locus_tag="VYN80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1540901.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16245..16751)
                     /locus_tag="VYN80_000968"
     CDS             complement(16245..16751)
                     /locus_tag="VYN80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1540902.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFCFICFSDFLSNSWLFQ
                     TKKEIPSR"
     gene            17359..19692
                     /locus_tag="VYN80_000969"
     CDS             17359..19692
                     /locus_tag="VYN80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1540903.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            19705..21168
                     /locus_tag="VYN80_000970"
     CDS             19705..21168
                     /locus_tag="VYN80_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1540904.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21168..>21551
                     /locus_tag="VYN80_000971"
     CDS             21168..>21551
                     /locus_tag="VYN80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1540905.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTE"
BASE COUNT         6848 a         4388 c         4010 g         6305 t
ORIGIN      
        1 aatatgaact tagttttatg acataataga cctatccact atatgaaagg aattgccaat
       61 gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg gtgtcgctgc
      121 cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact tgagagaact
      181 tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg ctgatgaaat
      241 gggctttgaa acaagacctg ttcaagcaga taaaacgctc tttgacatga gtgatgtccc
      301 ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact atgttgtcta
      361 tcaaacaaag aaagactatc tgattattgg tgatcctgac ccttctgtaa aaatcactaa
      421 aatgtcaaaa gaacgctttt tctatgaatg gactggagta gctatttttc tagctaccaa
      481 acccagctat caaccccata aagataaaaa gaatggtcta ctaagcaagc ttccttcctc
      541 tgattttcaa acaaaaatct ctcattgctt acattgttct ctcaagctta ttggtcacta
      601 ttatcaatat aggtggttct tactatctcc aaggaatctt ggatgaatac attccaaatc
      661 agatgaaatc aactttagga atcatctcag ttggtctggt tatcacctat atcctccaac
      721 aagtcatgag cttctccaga gattatctcc taaccgttct gagtcagaga ttaagtattg
      781 atgtgatttt atcctatatt cgccatattt ttgaacttcc catgtctttc tttgcgacac
      841 gtcgtacagg agaaatcatt tcacgattca cagatgctaa ctctattata gatgccttgg
      901 cttctaccat tctttctctt tttctggatg tttctattct gattcttgta ggaggcgtct
      961 tactggcaca aaaccctaat ctcttccttc tttctcttat ttccattcct atatacatgt
     1021 tcatcatctt ttcttttatg aaacctttcg aaaaaatgaa ccatgatgtc atgcaaagta
     1081 attctatggt tagctctgcc attatcgaag atatcaacgg gattgaaact ataaagtcgc
     1141 tcacgagtga agaaaatcgc tatcaaaata tagacagcga atttgtagat tatttggaaa
     1201 aatcctttaa gctcagtaaa tattctattt tacaaacgag tttaaagcag ggaacaaaat
     1261 tagttctgaa tatccttatc ctatggtttg gcgctcaatt agtcatgtcg agtaaaattt
     1321 ctatcggtca gctgattacc tttaacacac ttttttctta ctttacaact cctatggaaa
     1381 atattatcaa cctccaaacc aaactccaat ctgcgaaggt cgctaataac cgtttgaacg
     1441 aagtctatct agtcgaatct gaatttcaag ttcaagaaaa ccctgtccat tcacattttt
     1501 tgatgggcga tattgaattt gatgaccttt cttataagta tggttttgga cgagatacct
     1561 taacagatat taatctcacg attaaacaag gagataaggt tagcctagtt ggagttagtg
     1621 gttctggtaa aacaacttta gccaaaatga ttgtcaattt ctttgaaccc tacaaaggac
     1681 atatttctat caatcatcag gatattaaaa acattgataa aaaagtcttg cgccgtcata
     1741 ttaattacct accacaacaa gcctatatct ttaatggctc tatcttggaa aacttaactt
     1801 tgggcggtaa tcatatgatt agccaagaag atattctaag agcttgtgaa ttagctgaaa
     1861 ttcgtcaaga cattgaaaga atgcctatgg gctatcaaac tcagctctct gatggagctg
     1921 gtctatcagg aggacaaaag caacgaatag ccctcgctcg tgctctttta actaaagctc
     1981 ctgttttaat actagacgaa gctactagcg gtcttgatgt cttgactgag aaaaaggtta
     2041 tagataatct tatgtctcta actgataaaa ccattctctt tgtagcccat cgtctcagta
     2101 tagccgaacg aaccaaccgt gtcattgttc ttgaccaggg gaaaatcatt gaagttggta
     2161 gtcaccaaga gttaatgcag gcgcaaggtt tctaccatca tctattcaat aaataaggag
     2221 aatgtcatga atcctaatct ttttagaagc gtcgagtttt atcagagacg ttaccataac
     2281 tatgcgacag tgttcattat acctctttca ttactattta ctttcatctt gattttctcc
     2341 cttgttgcca caaaagaaat tactgttact tcccaaggag aaatcgcccc tacaagtgtc
     2401 attgcctcca ttcagtcaac cagtgataat cctatcctag ctaatcattt agtggcaaat
     2461 caagtagttg aaaaagggga cttactcatc aaatactctg aaacaatgga agaaagtcag
     2521 aaaactgcct tagcaactca attacaaaga cttgagaagc aaaaagaagg acttggaatt
     2581 ttgaaacaaa gcttagaaaa agcgactgat cttttttctg gcgaggatga atttggctac
     2641 cataatacct ttatgaattt tactaaacaa tcccatgata ttgaactggg tatcacaaag
     2701 actaacaccg aagtttcaaa tcaagctaat ctttccaata gcagttcatc agctattgaa
     2761 caagaaatta caaaagttca acaacaaatt ggagaatatc aagagttgag agatgctatc
     2821 ataaataaca gagcacgctt accaactggc aatccgcacc agtcaatttt gaatcgttat
     2881 cttgtagcct cacaaggaca aacacaagga actgcagagg agccattttt atctcaaatt
     2941 aatcaaagta ttgcaggtct tgaatcatct atcgcaagcc tcaaaattca gcaagctggt
     3001 atcggaagtg tagcaactta tgataacagt ttagcaacca aaattgaagt actccgcact
     3061 cagtttttac agacagcctc acagcaacaa ctaactgtgg agaatcaatt aacagaatta
     3121 aaagtacaac tagatcaagc cacacagcgt ttggaaaaca ataccttaac ctccccaagt
     3181 aaaggtatcg ttcatctgaa cagcgaattt gaaggtaaaa atagaattcc aactggtaca
     3241 gaaattgctc aaatattccc tgtcatcaca gatacaagag aagtactaat cacttactac
     3301 gtatcttctg actatctacc tctactagat aaaggacaaa ctgtaagatt aaaactggag
     3361 aagattggaa atcacggcac caccatcatc ggccaacttc agacaattga tcaaactcct
     3421 accagaacag agcaaggaaa tctctttaaa ttaaccgctc ttgcaaaact atctaacgag
     3481 gatagtaaac tcatccaata tggcttacaa ggtcgcgtca ctagtgtaac tacaaagaaa
     3541 acatattttg attatttcaa agataaaatt ttaacccatt ctgattaatt ttcagataac
     3601 actctataac tatttattat cttatcaaaa aggagaatca taacatggat aagaaacaaa
     3661 acctaacttc atttcaagaa ctaacaacta ccgaactcaa ccaaattaca ggtggaggat
     3721 tgtgggaaga tttattatat aacattaata gatatgctca ttacatcaca taagaacttc
     3781 atcatccaat acaactataa aaaaataaga ccgagaaaca agtactctcg gtcttatttt
     3841 tcatcattct gtatgtatca cagtaagtac ctgacgaaag acttgatttt gacaggtggt
     3901 atttagactg gtattaggat ggctttccac aatcttcatg acggtataga gaccaactcc
     3961 tctctcctcc cctttagaac tggctccaaa ggagaagatt tcagaaatat cgatgccctc
     4021 ttctttgatg gagttttcga tgataaaggt ctcctgtgct ccatttttta aaaaggcgat
     4081 tgaaacatga ggttgactag cttccacact ggcttcaata gcattgtcac aaaggataga
     4141 cacaatggtt agaaaatcaa gtagactcat cccctcgacc tgaatctcct caggaacttc
     4201 gacattaaag acaatgttct tatctctggc ttttaaaaat ttccctgcta gaagactttt
     4261 gagggcttta tcacgaatat ttaccaatct gcccaggtca tatttattgt tctgcaattt
     4321 ctgactggaa tcctttaaga cggagccata gacctctttt atctgctcca tatcctcctc
     4381 ttcaatgccc agacgtaagc tagtcaagag gttggtataa tcatgacgaa agctccgtac
     4441 ttccttgtaa agctcctcta tatgccgact atagcgttcc atatctctat agcgcagggc
     4501 ctgctcttgt tccaatctct catagagttt ttccttcaaa taggtatcca atttcttgat
     4561 aacccccata aaaaagagta ggtaaaagac taggatgaga tggcgaacag tctttgattg
     4621 aatactttgt tcatattcaa aaaaagacag actttccatg actagatagt agccacccat
     4681 tatccagtta atctgagtca gggacttttg aaaggcttta tcgagaatct cctttctcaa
     4741 gctagtaaaa tcgtagtcca accatttcaa aaaagctaga gaaatgaaga aattgaaaat
     4801 tattatacat aacccagtaa atgagtagcc atcatatact tgcccttgtc ccaaaaatgg
     4861 aagcacaaaa taggagactc ctctataaaa gagattcacc aatatcattg gaaagagacc
     4921 ataaaagaaa aggagttttt taggaagccc tctcaataat aagaaagata agcctatgcc
     4981 gtacaagggt tccataaaat aagataggta aacatttcct actatatagc taatcatcac
     5041 aaaaacaaag gccaacagta tcttaaaaag aaaggcctta aaaatcctct cgaaagtaag
     5101 atcaattcca tccaccttaa agaagatgac aatttctagt ccattagtaa caagtgtata
     5161 caacaatatc caagcaatgt tcataaattc tcctagctca gtgtaattta ttgatggcct
     5221 cagacacttc cctgacctta taacgggcga ttagacaact tccaccattg ggaaagaaaa
     5281 gcagtttttc tttcttatcc aaatgcacca catttgcagg attgatgaga aaagagcggt
     5341 ggcactgcaa gagacggggc tcctgcttga aaacctcctc taaactcgct gtaaattcca
     5401 gcctgtctgt cttggtatag agaataacac gatgggctct gggcgacgtt tcgagatagt
     5461 aaacctcttt aaaaggatac tgaaattggg caaattttga tttaaagtaa aagcaatctt
     5521 ccgccagact tttactatct tgactattgg catagaggag ggctgtctcg atccgagatt
     5581 caaactcctc tgctgacaag gccttatcaa tgtagtccaa agcagacact tggtagcgaa
     5641 aagacagggg cataaactcc gagtgagtcg tcacaaagac aatcagggca taaggatccc
     5701 gatcccgaat ctttctagcc acttccagtc ccttcatctc ttcatttcga atctcaatat
     5761 ccaaaaagaa tagctgatgg gcccccttct catgcacttc agccagcagt tggtccggct
     5821 tgccaaatac ctcaaaagag ctaggaatga tatgatgtgc tttcaaaagt ttctcaatcg
     5881 tcgtttcaat tctagtctat tgggaaaaat catcctctaa aacaaatatt ctcatcttct
     5941 tactctccct ctttcaacca tttttgacga atttctctat agcgacgtct ggcatacttg
     6001 acagcgtatt gaccttcttc tccgagaaaa aggattcttt cttgaaaatc aatcgatttt
     6061 aatttatcaa gattaaccaa acaatttcga tgacatctca tcaaggtttc cccacattgg
     6121 ttctcaagat tacttaaatt ttgaagcata ttaaaactag cttcttctgt aacaatctgt
     6181 acggtatggg ctttagttgg atgtgtttgg atatagtaga tatcgtcgat gtttacttta
     6241 tagactgttt tctgcgtctg aataatcata tatttcattg gattctcctc ctaagaaaag
     6301 aataacagaa aaggtctagc ccaagcacac ccaatcctaa acagtctttg aaaactccta
     6361 aaaggattat tttctacaac atcatgatga gcttacaact attttatcat aaaattcact
     6421 cagcgcaatt caagacattt caatgacaat taaagatttg aataaaaaaa aagaattaaa
     6481 agtgataaac taacagtatc aaaacacagt tgccagaagt aagactggca cccagattaa
     6541 aggaggcatt cccatgacag atacagaccc catcaaaaga gctcatactt tgattactga
     6601 cttaaacaaa gcctaccaag catgcaaaca ggcaagcgct gacgacgtcc gctttcagga
     6661 gcaattaaac tctattcttg gttttctagc caaggctgaa acagtggata atcgattctt
     6721 gattgaacta gaaaaatttt accagacttc cagtcttctc atgggactca gcgctcttga
     6781 tccagatgct ccaactcgcg ccgcttggcg ggcctatgac cgcttccact ttgaccaagt
     6841 caagaccaag ttaatactca atgaaaatca aagagcaaac taggaagcta gccgcaagct
     6901 gtacttgagt acggtaagac gacgctgacg tggtttgaag agattttcga agagtataag
     6961 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agttgagaca gactgaactc
     7021 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
     7081 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggctagttt
     7141 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
     7201 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
     7261 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
     7321 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
     7381 ggaaagtaag agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
     7441 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
     7501 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
     7561 gaaacgaagc aagacttgct tacgcttgct ttcttgggaa atgacatagt cccaatccag
     7621 tccagtttca gggaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
     7681 caataaaagc acatagagca gaaaaactgg caagccataa cccattgcta aaaataaagg
     7741 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
     7801 ttgacgcttg agatggagct tgatttcctc ttctccaact aagagaaaga gcttgtctgg
     7861 agcctccata taggtggcaa ttcctcccca aagtaaaagt aaaacagacg taattcctac
     7921 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     7981 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     8041 cagataacca agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     8101 aggtcctcct cttttgtcag agccaagtaa atatcattca aactagcctc aggcatatca
     8161 aaggcttcgc gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     8221 acaaaggcat cacacatctt ctccgccgaa tccagcacgt gggtactcat gagaatagac
     8281 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     8341 agaccaagga aaggctcatc cacgatgaaa agacttggat ccgccacaaa agcacagata
     8401 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     8461 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     8521 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     8581 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     8641 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     8701 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     8761 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     8821 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     8881 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     8941 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     9001 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatggatgct
     9061 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     9121 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     9181 tttcatactc acgttcacct tgtacctcgc tacagtgctg acgatgacct caagattgat
     9241 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     9301 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     9361 agttatctgg tcacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     9421 cgcgttcaag ctatcaagga cgatgtggat attatccaaa acagcctgca aatcattgac
     9481 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     9541 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     9601 taaaaagagc ccgacggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     9661 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     9721 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     9781 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     9841 aacaccgttt taaggttgtg gatagaactg acgaagtcag taaccatacc catgacaagc
     9901 cgacgctgac gtggtttgaa gaaatttttg aagagtatca ttcaccattt cacaactaaa
     9961 tcgaagactt tctgccgtaa tgaaaacacc tgaaacagct tggtttcggc tatccggaaa
    10021 ctttaagact taggttcaaa gtttaggtat ggaacttcga aggaggtcgc taccgtccgt
    10081 cattacttag gaaagtctta aaaaatctat aaagtaaaaa gagcccgaca gctctttttg
    10141 tttatactca atgaaaatca aaaagcaaac taggaaacta gccacgggct gctcaaaaca
    10201 ctgttttgag gttgtagata cgactgacga agtcagtcac atatatacgg caaggcaacg
    10261 ctgacgtggt ttgaagagat tttcgaagag tattattctc catcaaaggc atctttaata
    10321 tggtcaaaga agcctttttt ctttggattt actttcaagt caccagcagc cgcgaattct
    10381 ttcaaggcta ctttttggcg gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg
    10441 tattggtcac caactgcacc gccacgaagg ctcggtgccc ccttactacg taggcggaac
    10501 ttcttaccag tctgagttcc ctctggaata accaattcaa catcaccgtg aacagttgga
    10561 atatctactg tatcaccaag agccgcttgg acaaagttga ggttgagatt gtagaagata
    10621 gtcgttcctt cacgttcaaa cttgtcgcta gcttccacag aaactactac atacaagtca
    10681 ccataaggtc caccgttaaa gcctgcttca ccttgaccag cgaggcgaat ttgttgacct
    10741 gtttccacac cagcagggat tttcacatgt acgctatgag cttgtttctc atgacctgtt
    10801 ccatgacagg ttgtacatgg atatttgatt tcttttcctc gaccgtgaca gacatcacag
    10861 gttacttggc gacgcatcat accaagagga gtctgcgtat cgacgttaat gacaccagcg
    10921 ccatgacagc gtccacaagt gactggactt gtccctggct tagcaccaga tccattacat
    10981 gtacgacagc cagcttcacg atgatactta acttccttct cagttccgaa gatagcttct
    11041 tcaaaggtca aattgacacg atactggaga tcatctcctt ggcgaggagc gtttggattg
    11101 cgcgaagaac cgcctccgcc gaagaaactt gagaaaatat cctcaaaacc accgaagcca
    11161 cctgccccat tgaaaccgcc gaaaccacca gctccaccaa aaccaccatt ggcgcctgca
    11221 gcaccatact ggtcataggc agcacgtttt tggtcgtcac tcaaagtctc ataggcttct
    11281 tgaacttcct tgtacttgtc ctcagcacca ggctccttgt tgatatctgg gtgatatttt
    11341 ttggaaagct tacgataagc ctttttgatt tcgtctgccg aagcgttttt ggataccccc
    11401 agacgatcat aaaattcagt attgttcata attcaagata caaagggcgt caaacggaca
    11461 cagccatcta ggagttttga cgacggacgc ttacgtccta gaaaaaacta tctttttggc
    11521 acagtccata gcccgtgttc agttacgaac acccttattc ctttctattg atgttctgaa
    11581 tcgaaccacc atttaggtcg ggttcaattc ctttctttta tatttagtaa gaaattctga
    11641 aacccgtgtt cagttacgaa cactctttgt tcctttctat tgatgagaca aaacccaaac
    11701 aaatgagatt ccgtcccttt ttatcgaaaa acagaggctg ggttgcaacc tctgcaaata
    11761 agtaaattaa ctttattttg attttaaata tcctataagt acagaaatat ttatatattt
    11821 ttgtctcttc ttagtatttg cctatataat tccaattgat cataagaaat acaactactg
    11881 tccaaatcat accaattatg aacggtatta caaatttata tgtttgtatt tctaatgata
    11941 tcatagctga taccatcgct gatacaaatg cagacaacaa tactgaagta acaaaattcc
    12001 ttaccgcttc atcatttttg acacaactat catgctcagc taatataatt tctctatata
    12061 tagtcaaaca ttctactgga atattatcta taaaaccttt tgctgcttca tagttgtcag
    12121 cttgattaca aaaatcatta taaacttttc gtgtattttt tagatactca tcatccaata
    12181 cactcatctt atttttccgt aaactctccg tctacgacgt catcgcctgc gtttcctgtt
    12241 gcttgtgcgc cttctgctcc ttcttgagct tgttgcgctg ctgcggcttg ttcgtagagt
    12301 ttcacagcaa ggccttgagc tttttcgttc aacgcttcaa gtttcgcttt catgtcgtcc
    12361 aagttgttgt cttcttgagc tttcttaagg tcatcaaggg cagcttgggc agcgtcacgt
    12421 tctgcgtcga agcctttacc ttcagtttcc ttgattgtct tttcagtcgc aaagattgct
    12481 tggtctactt cattacgaag gtctacttct tctttacgtt tcttatcggc ttcagcgttt
    12541 gcttctgcat ctttcatcat gcggtcgatt tcttcgtcag tcaaacctga gttcgattgg
    12601 atgacaatag tttgttcttt ttgagttcca aggtctttgg ccttaacaga cacgataccg
    12661 ttcttgtcga tgtcaaatgt tacttcgatt tgaggaattc cacgaggtgc agctgggata
    12721 tcagtcaatt ggaagcgtcc aagagtcttg ttatctgctg ccattgggcg ttcaccttga
    12781 agaacgtgga tatcaacggc tggttggttg tctgctgctg ttgagaagac ttgtgattta
    12841 gatgttggga ttgtagtgtt gcgatcgata agttttgtaa atactccacc cattgtttcg
    12901 ataccaagtg acaatggcgt tacatcaaga aggacaacgt ccttgacatc accagtaatc
    12961 acaccacctt ggatagccgc acccatagca actacttcat cagggtttac tgatttgttt
    13021 ggttctttac cagtttcagc tttaacagct tcaacaacgg cagggatacg agttgaacca
    13081 ccaacaagga taacttcgtc aatttctgac aagctcaaac ctgcatctga aagggcttga
    13141 cgaactggaa cttttgtacg ttcaacaagg tcacgagtca aatcgtcaaa tttcgcacga
    13201 gtcaaagtca tttccaagtg aagaggtcca gcctcacctg cagtgataaa tggcaagctg
    13261 atttgtgttg aagttacacc agaaaggtct ttcttcgctt tttcagccgc atctttcaaa
    13321 cgttgcattg ccatcttgtc agtagacaag tcgataccgt tttctttctt gaattctgct
    13381 accaagtggt caatgatttt ttggtcaaag tcgtcaccac caagtttgtt gtcccctgca
    13441 gttgacaata cgtcgaagac accgtcaccc aattcaagga tagagacgtc gaatgtacca
    13501 ccaccaaggt caaataccaa gattttttct tctttgtcag tcttgtccaa accataagca
    13561 agagctgctg cagttggttc gttaacaata cgttctactt caagaccagc aattttacca
    13621 gcgtcttttg ttgcttgacg ttgagcgtcg ttgaagtaag ccggaactgt gataacagct
    13681 ttggttactt tctcaccaag gtagtcttca gcgtagcctt tcaagtattg aaggatcata
    13741 gctgagattt cttgtggagt gtattctttt ccatttgcag aaactttttc agaagttccc
    13801 atcttagatt tgatagagat aactgtatct gggtttgtga ctgcttgacg ttttgcagca
    13861 tcaccaacga tgatttctcc gtttttgaat gagactacag atggagttgt gcggttccct
    13921 tctgggtttg cgatgatttt gctttcagtt ccttcaagaa ctgcaactgc tgagtttgtt
    13981 gtacctaagt caataccgat aattttagac atgtgttttt ctccttgtta gttaattttc
    14041 tatttttctt tttgatactc ttcttaagtg gcgacgccgt cagagcttga cttcgtcaat
    14101 ctctttgact aaactttgag cctaaggtct caaaatttgc gcaaatagca ccacggcgaa
    14161 gagtatcgtc tttggttcgc ttcgctcact attacaaagc caaacatatt ttatctttct
    14221 tcatagttta ttgtcgtttc ggacaagttt tctattatgt aaattgcgac acgaggagtc
    14281 gaaatcgatt ttatttcgac gacgagttag taaggaagct aggcaaacgt catagcgatt
    14341 gccgttttct gacgagttgc tttagctacc gtcagaattg ccgaatcgaa cacgccctaa
    14401 gctctgagta aaaaagatag attttcttgt gttcatcgaa cacttcgtca atctcctatt
    14461 tttactgcga gctttttacg agctttgtat cttagttata caccactacc attgctgggc
    14521 gtaggatgcg gtcatggagt ttgtagcctt tttgaaagac ttgggcgatg gtatctactg
    14581 ggtgttcatc gtctgctggg agagtttgga tggccatatg gtagttatgg tcaaattcgc
    14641 catctgctgc gatttcttca attccttctt ctttcaaagc gtgaatcaag ctttcttgca
    14701 ccatcgccaa gcccttcttc acatcatctg tcaaaccttc aactgcaagt gcacgctcaa
    14761 ggttgtcaag agatggtaag attgcttttg ccaagtcctg gctacgataa cgttgcaagt
    14821 tttgacgttc ttcattggca cggcgttgga tattttgcat ttctgcatga gcgcgaagat
    14881 atttgttttc gaactcatct gcacgttcat ttgccaagtc caactcagac ttttcaggag
    14941 ttgtttcttc agctgtttcc acaacttcct cttcttgaac ttcttctact tcttcatttt
    15001 ttatatcttg ggccatgttc gcctccttta atgatttcaa tcttaatgta cttcgtaatg
    15061 attactgctg aggtagcggt aaaaatctgt caacttcatg gtcaaaacac ggttgaccac
    15121 attgacttga ttgattagct gttggtaatc cagattaact ggaccgataa tggctagaat
    15181 tccaactccc cgataaggaa tgaggaactt actactaatc accgctaggt cagctaaaca
    15241 ggactcttga ccgtctgcaa cacgaacatt ttgcatctga tcctcacgca acccctcacg
    15301 aatctccaag gccacctttt gcggttggtc aaagaactga taggctgcta gattggcaaa
    15361 attcaagaga tggaccttgc ccgccatcac aatgttttcg ttgaacattt ccttaaagat
    15421 gtgttcaaag agatcgatga cattatccgt tgttgtaaag taacgctgga taatctgcgg
    15481 aatctccgtc cgaatcttgt agtgaatatc taaaacggtg tgaccgagga aacgttcctg
    15541 aatgatgctc ttcagtttca gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg
    15601 actagtaacc gttcgcgact cgtctagggt aaataccgcc aaggctgtat gttgccccaa
    15661 aacaacgata tcaaaggctg tcaaacgttg cctgctcggc tcaacatcca gtgccactac
    15721 cgtacagcca ctcaggtctg ttagcaagtt agcagcctct tgcagaatat cctccaattt
    15781 gaagaattcc tgatcaaagg ctttgacaat ctcatatacc tcattttcag ccagtcggtc
    15841 aaaatccagt gagtgtttca catagtactg aaaaccagca acacttggca tccgaccgct
    15901 tgaagtatga gccttctcaa gcaacccttg tttttctagt tccgccatgt cattacgaat
    15961 ggttgcactg ctagagttaa tagactcttg caaggttttt gatccgacag gttcgtgcgt
    16021 tttggtaaag atgtcaataa tcagatttaa aatatcctgc tgacgctctg taaccatctc
    16081 atcactcctc tctgtctgat caattagcac tcaatgcatc caagtgctaa ctcatgattc
    16141 tattatacac ccttagaaga gaatgtcaag acaaaaactc aaaaaattag cactctttta
    16201 tcaagagtgc taaaaatcaa tctgaagacc tagaaccttg ctattcatct actcggtatt
    16261 tcctttttag tctgaaataa ccatgaatta gataagaaat cagaaaagca tataaaacaa
    16321 aaaataagaa agaaagacat agaaagatcc tctctaaaca aactcataca aacaacaaga
    16381 ccacctaaga aagtagctgt acaggaacga agtctagatc gcataacttc ccatttgagc
    16441 cccttactca tatagacttg atcctctgga agtaaatcag aagacgattt acgaagaatc
    16501 gaacaagaac ctgctcctat caattcccaa cctctatctc taaaatcttg cagttcacgc
    16561 ttatgctctt taagaaatct agaatcatag atacgataga taacatcgtc cggttgacct
    16621 tgctcaaaat agaacaaacc aaaacgactc gttctatacc tccaaccttt caaatgcatc
    16681 tcatgtaaat attcttcttt cttgtccaaa tcaacaatgg tgaaaatccg aaattctact
    16741 ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt tattagataa
    16801 ttctttccac ttttgactca atctccaaaa aatataagaa atctgaatcg caaaaactat
    16861 caataaaacc caatctatta tgaaaatcaa aaacactttc caactgaaag aactacctcc
    16921 agtaacaaac tttgagaaaa acggtagtag agctaaaaag agaagtaaaa taggaagcat
    16981 ccgcattgtt aaaatccgtt tggcataaaa aaatctttat ttaaactaaa atattatagc
    17041 aaaatttacg ccagtttttg aacggctgat gtagatattt tatactttca aaatgtttaa
    17101 atgtgattat ttatttttga aaaatagctc accagcccga ctgaaagtgc ttatagaatg
    17161 ataataagtc gcctgccgaa aacagcggaa aatagcggtg ttatgcggag ataatctgac
    17221 gcgatgcgaa agtatattgc atacttattt tcaacaattt agcagagtat ttttataagt
    17281 gtgatataat agaagtataa tttgttctga tagtttattt tatggagaag tagattttta
    17341 gaatgcggag ggttcaatat ggttgagttt ataaagtcta agaaagaaat gagtgaggag
    17401 gatattaaag caaatttcat cactcctgct attgtatcca aaggatggaa aaatggtgag
    17461 catatcgctt acgaagaata cttcactgat ggtcgaattg aagttagagg agataaggct
    17521 cgtcgtaaag aaggaaaaaa atcagactat tcactgtatt accaatttgg aactcgaatt
    17581 gcaattgttg aggcaaagga taataaacac agcgttcgag caggattaca acaagctatt
    17641 gaatatggag agattttaga tgttccattt gtttattctt cgaatggtga tggctttatt
    17701 gaacacgacc gtatcacgag agaagaacgt gagctggagt tagacgaatt ccctactcgt
    17761 gaagaattat tttctcgtat gacgaaggaa aaaggattga cgtacgaaat tacagaagct
    17821 atctcaactc catactatac agacgccttc tcaatgaaaa cgccacgcta ttatcagcaa
    17881 atagctatca accgtactat tgaaacagtt gccagaggac aaaaacgagt aatgtttgtg
    17941 atggcaacag gaacggggaa aacgttcatg gcttttcaaa ttattcatcg ccttcgaaaa
    18001 gctggtttgg ctaaacgagt tttattctta gcagatagaa acatcttagt agaccaaacg
    18061 atggctgaag actttaggcc attcgaaaag gtaatgacga aaattacacc aaaacttttg
    18121 actgctcctg aaaaattaaa ttcttttgaa atttatctag ggctttatca gcaactaact
    18181 ggtgaagatg gaactgaaac acattatcaa aaatttgaca aagacttctt tgatttaatc
    18241 gtaattgatg aagcacaccg tggttcagct aaggaaaaca gtaactggcg taaggtaatt
    18301 gattatttca gttctgcgac acagattggg atgaccgcta ctcctaaaga aaccaagaat
    18361 gcttccaata cggaatactt tggtgagcca atctatactt atagtttaaa acagggaatc
    18421 gaggatggtt ttttggctcc atatcgtgtt atgagggtta atttagatgt ggatgtggat
    18481 ggttatcgtc cagaaactgg aaaagttgat gctaacggac aattaataga agataggtac
    18541 tacggcagga aagattttga taaaaccatt gtcattgatg atagaacgca aagagttgcc
    18601 aagtttgttt ctgattatat gaagcaaaac aatgcacgat ttgataaaac aattgttttt
    18661 tgtgttgata ttgaccatgc cgagcgaatg cgtgctgcac ttgtaaaaga gaatctagac
    18721 ttagtccaag aagactatcg ttatgtcatg caagtaactg gtgacaacgc tgaaggaaaa
    18781 gctcaactgg ataactttat ggatgtcaat tctaattttc ccgctattgt aacaacgtct
    18841 aaattattaa cgacaggagt taatgctaaa acatgtcgtt tgattgtttt agactctaat
    18901 atccaatcca tgactgaatt taaacaaatt attggtcgtg gcacacgtct ttatcctcaa
    18961 aaggggaaag aattttttac gattattgat tttcgaaatg ttaccaattt gtttgctgac
    19021 cctgattttg atggtgatcc agtgaaggtg ctggaaacag gtgcgaaaac agtcagtggt
    19081 tctacgcccg gtttcgtaga tgaggaaggt gacccagtag aaaaatatat cgttacagac
    19141 aagcaggtta ccattcttaa ttctactgtt caagtattgg atgaaaacgg gaaactgatt
    19201 accgaaagcc tgaccgacta cactcgaaag aatatcttag gtagctacgc cactttgaac
    19261 gattttatca cagtttggca tacggcagat aaaaagaagc ttatcttaga cgaactttat
    19321 aaaaaaggag tttatctaga tgctattcga gagtcggagg gaatatcaga acaagaaatc
    19381 gatgattttg atttactcct aaaacttgcc tatggtcaaa aagaattaac caaaacggaa
    19441 cgtatcaata aactcaaaca aagcggatat ttatataaat atagtgagga agcgcgtgct
    19501 gttttggaaa ttttactgaa caaatacatg gataaaggta ttggagaact cgaaagcatt
    19561 gaaacattaa aacttccaga atttcagata tatggtggaa ccttcaaaat catcaatact
    19621 tattttggag ataaaaaacg atatttacaa gcaattaaag aattggagca agagctattt
    19681 acagtagctt aatgaaagga aagtatgtca attacatcat ttgtaaaaag aattcaagat
    19741 atcactcgaa acgatgctgg tgttaatggt gatgctcaac gtattgagca aatgtcttgg
    19801 ttattattct taaaaattta tgatagccgt gaaatggttt gggaattaga agaagacgag
    19861 tatgagtcaa ttatcccaga ggaattaaaa tggcgaaatt gggctcatgc tcaaaatggg
    19921 gaacgggtat tgacaggcga tgaattactt gattttgtca ataacaagtt attcaaagag
    19981 ttgaaagagc ttgaaataac ttcaattatg cctattcgaa aaacgattgt taaatcagct
    20041 tttgaagatg cgaacaacta tatgaaaaat ggcgtcttgt tacgccaagt catcaatgtt
    20101 attgatgaag ttgatttcaa tagccctgaa gatcgtcatt cgtttaatga tatttacgaa
    20161 aaaattctta aagatattca aaatgctggg aactcaggag aattttatac gccacgtgca
    20221 gcgactgatt ttattgccga agttcttaac ccaaaacttg gagaatcaat ggcagacctt
    20281 gcttgcggaa caggaggctt cttgacttcg actctgaacc gtttaagtag tcaacgtaaa
    20341 actagtgaag ataccaaaaa atataataca gctgtttttg gtattgaaaa gaaagcattt
    20401 cctcatcttt tagcagttac aaatctgttt cttcacgaaa ttgatgaccc taaaattgtt
    20461 catggaaata ctttggagaa aaatgttcgt gaatatacgg atgatgaaaa atttgacatt
    20521 attatgatga atccaccttt tggagggtca gaattagaaa caataaaaaa taactttcca
    20581 gcagaattac ggagttctga aacagctgat ttatttatgg ctgtcattat gtatcgtttg
    20641 aaagaaaatg gtcgtgttgg agttatttta cctgatggtt ttctatttgg tgaaggtgta
    20701 aaaactcgct tgaaacaaaa actggtagat gagttcaact tgcatacgat tattaggttg
    20761 cctcatagtg tctttgcacc gtatacagga atccatacga acattctttt ctttgataaa
    20821 acaaagaaaa cagaagaaac ttggttttat cgtttagata tgccagatgg ttataaaaat
    20881 ttctcgaaaa ctaagccgat gaagtcagaa cacttcaatc ctgttcgtga ctggtgggaa
    20941 aatcgtgaag agattctgga aggtaagttc tacaaatcta aatcatttac acctagtgaa
    21001 ttggctgagt tgaattataa tttagaccag tgtggttttc caaaagagga agaggaaatc
    21061 ttaaatccct ttgagttgat tcagaattat caagcggaaa gagcaacttt aaatcataag
    21121 attgataatg tattagctga tattttgcag ttgttggagg acaaataatg acaccagaac
    21181 aacttaaagc aagtattctc caaagagcga tggaagggaa attagtgccg caaaatccca
    21241 atgacgaacc tgcaagtgaa ttattaaaga gaattaaagc tgaaaaagaa aaacttatca
    21301 gtgaaggaaa aatcaaacga gataaaaagg aaactgagat atttcgtggt gatgatggga
    21361 aacattatgg gaagtttgct gatggaagta ctcaagaaat tgatgttcct tatgatattc
    21421 ctgatacttg ggagtgggtg aggttttcta cattggttga aattgtcaga ggtggctctc
    21481 cacgaccaat caaagattat cttacttctg aagtagatgg aataaattgg ataaaaatag
    21541 gtgatactga a
//
LOCUS       DAVLWA010000026        20938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLWA010000026 DAVLWA010000000
VERSION     DAVLWA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.026"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(1..232)
                     /locus_tag="VYN80_000972"
                     /pseudo
     CDS             complement(1..232)
                     /locus_tag="VYN80_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            551..2533
                     /locus_tag="VYN80_000973"
     CDS             551..2533
                     /locus_tag="VYN80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1540906.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2834..8146
                     /locus_tag="VYN80_000974"
     CDS             2834..8146
                     /locus_tag="VYN80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1540907.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVAAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLPTDKEKMYYFNTQTGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTRYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKVKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPIVEKPEFKLRSLASVQGKTPDYKQEID
                     RPDRPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(8313..10472)
                     /locus_tag="VYN80_000975"
     CDS             complement(8313..10472)
                     /locus_tag="VYN80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1540908.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10469..11065)
                     /gene="recU"
                     /locus_tag="VYN80_000976"
     CDS             complement(10469..11065)
                     /gene="recU"
                     /locus_tag="VYN80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1540909.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            11131..11658
                     /locus_tag="VYN80_000977"
     CDS             11131..11658
                     /locus_tag="VYN80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1540910.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11728..12057
                     /gene="gpsB"
                     /locus_tag="VYN80_000978"
     CDS             11728..12057
                     /gene="gpsB"
                     /locus_tag="VYN80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1540911.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            12078..12458
                     /gene="rnpB"
                     /locus_tag="VYN80_000979"
     ncRNA           12078..12458
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYN80_000979"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12543..13700
                     /locus_tag="VYN80_000980"
     CDS             12543..13700
                     /locus_tag="VYN80_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1540912.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQNLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13713..15107
                     /gene="mapZ"
                     /locus_tag="VYN80_000981"
     CDS             13713..15107
                     /gene="mapZ"
                     /locus_tag="VYN80_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1540913.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTVNQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            15183..16607
                     /gene="gndA"
                     /locus_tag="VYN80_000982"
     CDS             15183..16607
                     /gene="gndA"
                     /locus_tag="VYN80_000982"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1540914.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16619..17308
                     /locus_tag="VYN80_000983"
     CDS             16619..17308
                     /locus_tag="VYN80_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540915.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17406..18425
                     /gene="cbpC"
                     /locus_tag="VYN80_000984"
     CDS             17406..18425
                     /gene="cbpC"
                     /locus_tag="VYN80_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1540916.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPITGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(18543..19775)
                     /locus_tag="VYN80_000985"
     CDS             complement(18543..19775)
                     /locus_tag="VYN80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1540917.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19786..20238)
                     /locus_tag="VYN80_000986"
     CDS             complement(19786..20238)
                     /locus_tag="VYN80_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540918.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNSQLSWCKGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(20306..20643)
                     /locus_tag="VYN80_000987"
                     /pseudo
     CDS             complement(20306..20643)
                     /locus_tag="VYN80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         6502 a         3791 c         4684 g         5961 t
ORIGIN      
        1 aggctttatc ttcaactgtt agcggtttga gtttgctgga tttacgtgga gtttgtgctt
       61 gaggatatat cttcatgagc ccttgataac cactgtcagc caagatttta ccagcttgtc
      121 cgatatttct gcgactcatt ttgaacaact ttatatcatg acaatagttc acagcgatat
      181 ccaaagaaac aattctccct tgacttgtga caatcgcttg agccttcata gcgagaaata
      241 tctatcctat cttctagaat gtcttccaaa cgaggaaact ctcgtaaaca aagaggtttt
      301 agaggcctat ttaccgtgga ctaaagttgt acaagaaaag tgcaaataag aaatctccag
      361 attaggaact atccgtgagt tctctagtct ggagattttt caatatactt cgttattggg
      421 cgcttacaat gatattcata ttttttgcaa agatgttgtt tgaaaaataa ttttcaaaaa
      481 ttctgaaaat tctgttgaca actttctgaa aagagtctat aatggagaga aagttttaaa
      541 ggagaaaatg atgaaaagtt caaaactatt tgcccttgcg ggcgtgacat tattggcggc
      601 gactacttta gctgcatgct ctggatcagg ttcaagtact aaaggtgaga agacattctc
      661 atacatttat gagacagacc ctgataacct caactatttg acaactgcta aggctgcgac
      721 agcaaatatt accagtaacg tggttgatgg tttgctagaa aatgatcgct acgggaactt
      781 tgtgccgtct atggctgagg attggtctgt atccaaggat ggattgactt acacttatac
      841 tatccgtaag gatgcaaaat ggtatacttc tgaaggtgaa gaatacgcgg cagtcaaagc
      901 tcaagacttt gtaacaggac taaaatatgc tgctgataaa aaatcagatg ctctttacct
      961 tgttcaagaa tcaatcaaag ggttggatgc ctatgtaaaa ggggaaatca aagatttctc
     1021 acaagtagga attaaggctc tggatgaaca gacagttcag tacactttga acaaaccaga
     1081 aagcttctgg aattctaaga caaccatggg tgtgcttgcg ccagttaatg aagagttttt
     1141 gaattcaaaa ggggatgatt ttgccaaagc tacggatcca agtagtctct tgtataacgg
     1201 tccttatttg ttgaaatcca ttgtgaccaa atcttctgtt gaatttgcga aaaatccgaa
     1261 ctactgggat aaggacaatg tgcatattga caaagttaaa ttgtcattct gggatggtca
     1321 agataccagc aaacctgcag aaaactttaa agatggtagc cttacagcag ctcgtctcta
     1381 tccaacaagt gcaagtttcg cagagcttga gaagagtatg aaggacaata ttgtctatac
     1441 tcaacaagac tctattacgt atctagttgg tacaaatatt gaccgtcagt cctataaata
     1501 cacatctaag accagcgatg aacaaaaggc atcgactaaa aaggctctct taaacaagga
     1561 tttccgtcag gctattgcct ttggttttga tcgtacagcc tatgcctctc agttgaatgg
     1621 acaaactgga gcaagcaaaa tcttacgtaa tatctttgtt ccaccaacat ttgttcaagc
     1681 agatggtaaa aactttggcg atatggtcaa agagaaattg gtcacttatg gggatgaatg
     1741 gaaggatgtt aatcttgcag attctcagga tggtctttac aatccagaaa aagccaaggc
     1801 tgaatttgct aaagctaaat cagccttaca agcagaaggt gtgacattcc caattcattt
     1861 ggatatgcca gttgaccaaa cagcaactac aaaagttcag cgcgtccaat ctatgaaaca
     1921 atccttggaa gcaactttag gagctgataa tgtcattatt gatatccaac aactacaaaa
     1981 agacgaagta aacaatatta catattttgc tgaaaatgct gctggcgaag actgggattt
     2041 atcagataat gtcggttggg gtccagactt tgccgatcca tcaacctacc ttgatatcat
     2101 caaaccatct gtaggagaaa gtactaaaac atatttaggg tttgactcag gggaagataa
     2161 tgtagctgct aaaaaagtag gtctatatga ctacgaaaaa ttggttactg aggctggtga
     2221 tgaggctaca gatgttgcta aacgctatga taaatacgct gcagcccaag cttggttgac
     2281 agatagtgct ttgattattc caactacatc tcgtacaggg cgtccaattt tgtctaagat
     2341 ggtaccattt acaataccat ttgcattgtc aggaaataaa ggtacaagtg aaccagtctt
     2401 gtataaatac ttggaacttc aagacaaggc agtcactgta gatgaatacc aaaaagctca
     2461 ggaaaaatgg atgaaagaaa aagaagagtc taataaaaag gctcaagaag atctcgcaaa
     2521 acatgtgaaa taactgttgc aaaatataag aaaggattta gtatttctct tgaatgctga
     2581 atcctttttt acatttgtaa agaaagattc taaatgtact gacccccaaa agttggagcc
     2641 tctttttgtc agaatagaga aaatttttgt taattttact tgtttcctat tgctttctca
     2701 gctattattt gttatattaa aagtataatt attttttatt tatcagggtt aagcattgca
     2761 ctttcagagg aaggagtatt ttttaaaaag aaaatgtaaa cgcttgctca aaaatgaaag
     2821 gatttagaag tttatgaata aaggattatt tgaaaaacgt tgtaaatata gtattcggaa
     2881 attttcatta ggtgttgctt ctgttatgat tggagctgca ttctttggga caagtccggt
     2941 tcttgcagat agcgtgcagt ctggttccac ggcgaactta ccagctgatt tagctactgc
     3001 tcttgcaaca gcaaaagaga atgatgggcg tgattttgaa gcgcctaagg tgggagaaga
     3061 ccaaggttct ccagaagtta cagatggacc taagacagaa gaagaactat tagcacttga
     3121 aaaagaaaaa ccggctgaag aaaaaccaaa agaggataaa cctgcagctg ctaaacctga
     3181 aacacctaag acggtaaccc ctgaatggca gacggtagag aaaaaagaac aaaagggaac
     3241 agtcactatc cgagaagaaa aaggtgtccg ctacaaccaa ctatcctcaa ctgctcaaaa
     3301 tgataacgca ggtaaaccag ccctgtttga aaagaagggc ttgaccgttg atgccaatgg
     3361 aaatgcaact gttgatttaa ccttcaaaga agattctgaa aagggcaaat cacgctttgg
     3421 tgtcttcctg aaatttaaag ataccaagaa caatgttttt gtcggttatg acaaggatgg
     3481 ctggttctgg gaatacaaaa ctccaggtaa tagcacatgg tataaaggca accgtgtagc
     3541 tgcaccagaa ccgggttctg taaaccgtct ttctatcact ctcaagtcag atggacaact
     3601 caatgccagc aacaacgatg tcaatctctt tgacacagtg actctaccag ctgcggtcaa
     3661 tgaccatctt aaaaatgaga agaagatcct tctcaaggca ggaacttatg gcaatgaccg
     3721 tacggttgtc agcgttaaaa cagacaatca agaaggcgta aaagcggatg atactcctgc
     3781 ccagaaagaa acaggtccgg ttgttgacga tagcaaggtg acttatgata cgatccagtc
     3841 taaggtcctc aaagcagtga ttgaccaagc cttccctcgt gtcaaggaat acaccttgaa
     3901 cgggcatact ttgccaggac aagtgcaaca gttcaaccaa gtctttatca ataaccaccg
     3961 aatcacccct gaagtcactt ataagaaaat caatgagaca acagcagagt acttgatgaa
     4021 gcttcgcgat gatgctcact taatcaatgc ggaaatgaca gtacgcttgc aagttgtgga
     4081 caatcaattg cactttgatg tgaccaagat tgtcaaccac aatcaagtca ctccaggtca
     4141 aaagatcgat gacgaaagaa aactactttc ttctattagt ttcctcggca atgctttagt
     4201 ctctgtttct agtgatcaaa ctggtgctaa gtttgatggg gcaaccatgt caaacaatac
     4261 gcatgtcagc ggagatgatc atatcgatgt aactaatcca atgaaggatt tggctaaggg
     4321 ttacatgtat ggatttgttt ctacagataa gcttgctgct ggtgtttgga gtaactctca
     4381 aaatagctat ggtggtggtt cgaatgactg gactcgtttg acagcttata aagaaacagt
     4441 cggaaatgcc aactatgtag gaatccacag ctctgaatgg caatgggaaa aagcttataa
     4501 gggcattgtt ttcccagaat acacgaagga acttccaagt gctaaggttg ttatcactga
     4561 agatgccaat gcagacaaga aagtcgattg gcaagatggt gccattgctt atcgtagcat
     4621 tatgaacaac cctcaaggtt gggaaaaagt taaggatatc acagcttacc gtatcgcgat
     4681 gaactttggt tctcaagcac aaaacccatt ccttatgacc ttggatggta tcaagaaaat
     4741 caatctccac acagatggtc ttgggcaagg tgttctcctt aaaggatatg gtagcgaagg
     4801 tcatgactct ggtcacttga actatgctga tattggtaag cgtatcggtg gtgtcgaaga
     4861 cttcaagacc ctaattgaga aggctaagaa atatggagct catctaggta tccacgttaa
     4921 cgcttcagaa acttatcctg agtctaaata cttcaatgaa aaaattctcc gtaagaatcc
     4981 agatggaagc tatagctatg gttggaactg gctagatcaa ggtatcaaca ttgatgctgc
     5041 ctatgaccta gctcatggtc gtttggcacg ttgggaagat ttgaagaaaa aacttggtga
     5101 cggtctcgac tttatctatg tggacgtttg gggtaatggt caatcaggtg ataacggtgc
     5161 ctgggctacc cacgttcttg ctaaagaaat taacaaacaa ggctggcgct ttgcgatcga
     5221 gtggggccat ggtggtgagt acgactctac cttccatcac tgggcagctg acttgaccta
     5281 cggtggctac accaataaag gtatcaacag tgccatcacc cgctttatcc gtaaccacca
     5341 aaaagatgct tgggttgggg actacagaag ttatggtggt gcagccaact atccactgct
     5401 aggtggctac agcatgaaag actttgaagg ctggcaagga agaagtgatt acaatggcta
     5461 tgtaaccaac ttatttgccc atgacgtcat gactaagtac ttccaacact tcactgtaag
     5521 taaatgggaa aatggtacac cggtgactat gaccgataac ggtagcacct ataaatggac
     5581 tccagaaatg cgagtggaat tggtagatgc tgacaataat aaagtagttg taactcgtaa
     5641 gtcaaatgat gtcaatagcc cgcaatatcg cgaacgtaca gtaactctca acggacgtgt
     5701 catccaagat ggttcagctt acttgactcc ttggaactgg gatgcgaatg gtaagaaact
     5761 tcctactgat aaggaaaaga tgtactactt caatacgcag actggtgcaa caacttggac
     5821 ccttccaagc gattgggcaa agagcaaggt ttacctttac aagctaactg accaaggtaa
     5881 gacagaagag caagaactaa ctgtaaaaga tggtaaaatt accctagatc ttctagcaaa
     5941 tcaaccatac gttctctatc gttcgaaaca aaccaatcct gaaatgtcat ggagtgaagg
     6001 catgcacatc tatgatcaag gatttaatag cggtactttg aaacattgga ccatttcagg
     6061 cgatgcttct aaggcagaaa ttgtcaagtc tcaaggggca aacgatatgc ttcgtattca
     6121 aggaaacaaa gaaaaagtta gtctcactca gaaattaact ggcttgaaac caaataccag
     6181 gtatgccgtt tatgttggtg tagataaccg tagtaatgcc aaggcaagta tcactgtgaa
     6241 tactggtgaa aaagaagtga ctacttatac caataagtct ctcgcgctca actatgttaa
     6301 ggcttacgcc cacaatacac gtcgtgacaa tgctacagtt aacgatacaa gttacttcca
     6361 aaacatgtac gccttcttta caacaggatc ggacgtctca aatgttactc tgacattgag
     6421 tcgtgaagct ggtgatcaag caacttactt tgatgaaatt cgtacctttg aaaacaattc
     6481 aagcatgtat ggagacaagc atgatacagg taaaggcacc ttcaagcaag actttgaaaa
     6541 tgttgctcag ggtatcttcc catttgtagt gggtggtgtc gaaggtgtcg aagacaaccg
     6601 cactcacttg tctgaaaaac acgatccata tacacaacgt ggttggaatg gtaagaaagt
     6661 cgatgatgtt atcgaaggaa attggtcact caagacaaat ggactagtga gccgtcgtaa
     6721 cttggtttac caaaccatcc cacaaaactt ccgttttgaa gcaggtaaga cctaccgtgt
     6781 aacctttgaa tacgaagcag gttcagacaa tacctatgct tttgtagtcg gtaagggaga
     6841 attccagtca ggtcgtcgtg gtactcaagc aagcaacttg gaaatgcatg aattgccaaa
     6901 tacttggaca gattctaaga aagccaagaa ggtaaccttc ctcgtgacag gtgcagaaac
     6961 aggagatact tgggtaggta tctactcaac tggaaatgca agtaatactc gtggtgattc
     7021 tggtggaaat gccaacttcc gtggttataa cgacttcatg atggataatc ttcaaatcga
     7081 agaaattacc ctaacaggta agatgttgac agaaaatgct ctgaagaact acttgccaac
     7141 ggttgccatg actaactaca ccaaagagtc tatggatgct ttgaaagagg cggtctttaa
     7201 tctcagtcag gccgatgatg atatcagtgt ggaagaagcg cgtgcagaga ttgccaagat
     7261 tgaagccttg aagaatgctt tggttcagaa gaaaacggct ttggtagcag atgactttga
     7321 aagtttggat gcgccagctc aaccaggtga aggtcttgag aatgcctttg atggcaatgt
     7381 gtctagtcta tggcatacat cttggaatgg tggagatgta ggcaagcctg caaccatggt
     7441 cttgaaagaa ccaactgaaa tcactggact tcgttatgtt ccacgtggat caggttcaaa
     7501 tggtaacttg cgtgatgtga aacttgttgt gacagatgag tctggcaagg agcatacctt
     7561 tactgcaact gattggccag ataacaataa gccaaaagac attgactttg gtaagacaat
     7621 taaggttaag aaaattgtcc ttacaggtac taagacttac ggagatggtg gcgataaata
     7681 ccaatctgca gcggaactta tctttactcg tccacaggta gcagaaacac ctcttgactt
     7741 gtcaggctat gaagcagctt tggctaaggc tcagaaatta acagacaaag acaatcaaga
     7801 ggaagtagct agcgttcagg caagcatgaa atatgcgacg gataaccatc tcttgacgga
     7861 aagaatggtg gaatactttg cagattatct caaccaatta aaagattctg ctacgaaacc
     7921 agatgctcca attgtagaga aacctgagtt taaacttaga tctttagctt ccgttcaagg
     7981 taagacgcca gattataagc aagaaataga tagaccagat agaccagaaa cacctgaaca
     8041 aatcttgcca gcaacaggtg agagtcaatc tgacacagcc ctcttcctag caggtgttag
     8101 cctagcccta tctgctctct ttgtagtaaa aacgaagaaa gactagtatt tagtaaaacc
     8161 tcttaacaag attacggaag cagtctctat cttttccaat gaggtttata gtacagaaaa
     8221 agcctgagaa gatgtcttct caggcttttg ttaagcacat aaatacaata gtgctatgac
     8281 aaaatcaccc agaaaaatct gggtgataaa tgttatggtt gtgctggttg aggattctga
     8341 ttttgttgat caggggttgt atttgattgt tgcgtattat tgttaggatt ggtagtcgta
     8401 ctattatttg tgcttggagt ggttgagcta gactgtgaag ttgaactatc tgatgatgag
     8461 cttgaacttt cagttgatgg gggttgttgt ggagcaggtg agttccacgt agaacgagca
     8521 ccatttttaa atacgaattc tccatttctg tagagcccct ctggtatatt ccaatcctct
     8581 ggattgcttc cttcagacag gtaggtcatc atagagcggt aaactttggc agcgaccgta
     8641 aggccattgc ctacaagtgg tgtcagacgg ttagaatagc ctgtccatac agccattgaa
     8701 tatttacgcg tatagccagc aaatagttcg tcaggtgcta caaattgaga ggtcttgatg
     8761 tggttttcaa tttcctcgtc tgtatagtta gaggttcctg ttttaccagc ctgagggagc
     8821 caagcaagat aggcatttcg tccagttcca taactcaaga ctgttttcat catgtcggtc
     8881 atcatatagg ctgtcgtttc cttcatggca cgagttccga cattagagaa ctctttttca
     8941 ctcccatcac taaagacgac tttatggata tacattggtt tatagtaagt tccaccattt
     9001 gcaaaggcag cgtaagcagc agccatcttt tcactacttg ctccatattt tttgtctgat
     9061 tcggttgtgt tacttgaaat ggcatttgag tagtgaatac ttgggtagtc gattcctaga
     9121 ccatttagga aagtcttggc gcggttgagt ccgaccttgt ttagagtttc cacggctggg
     9181 acgtttcgcg attgttgcag ggcgtattgc aaggtgatgt tgccaaagta gcccctatcc
     9241 cagttataaa caggagtatt tgtcccaggg tagttatagg gctcatcgtg aacgatagta
     9301 gcagttgaat cgtagacacc gtactccaag gcaggagcat agtctgtgat cggtttcata
     9361 gttgatcccc agtcgcggtt tgtttctact gcttggttaa ttccgaagga aacattactt
     9421 gactgatggc gtgctcctag ctgggcaatg actttaccgt tagaaacatc aacaatggta
     9481 gaagcgactt gcaattcatc gtctggatag gcaacgtatt cgtctgtatt gtaaatatcc
     9541 cacagatgtt tttgagcttc ttggtctaca tttgtgtaga catccatccc agttgtgagt
     9601 aggttatagc ctgtttcttc ttcaacttga ttgatgactt ccttgaggta attatccatg
     9661 taagcagggt aattacttgc tgatttgaga ctttgtagcc catcagtaat tggtgtattg
     9721 actgctttct catactgttc agcagagatg tagccttgat ttttcatttc agataagacc
     9781 aagtttcggc ggtcttgggc tgcttctgga tgtgaatagg ggtcatattg gtttggtgcc
     9841 tgaggcattc cagccagcaa ggctaactga ggtaaactta aattattgag gtctttacca
     9901 tagtagtttt gagctgctgt ctgcattcca tagttcccat tagacatgta gaccttattt
     9961 atatagtagg tcaagatttc ttgcttggtt gctttttgtt ctaactgaat cgctaaccaa
    10021 gcttcctgag ccttacgaga aatagtctgg tcggaagtcg aagttgaaaa gtaagtcaac
    10081 ttaatcaact gttgggtgag agttgatcca ccttggaggg aattgctttg cagattgcgc
    10141 aagaaagctc ccaggatacg gatggtatca atccccctgt ggtcgaagaa gcgatggtct
    10201 tcgatagaaa cgattgcctt aaccaaatct gtgggaatat cattagcttg ggcattgacg
    10261 cggcgttcag aacccaagtc agcaatgagt tgatttttat tgtcgtagat tttactagaa
    10321 gttgttgcaa ctagtttact ctcggatagg ctaggagcct tgctaacgta gtagaaaaaa
    10381 actcctccgc ctaagacaat ggctgcgata accaagctta agaagctaat gctcagatac
    10441 ttgattaggc gcagaatcgt tggtttgttc atcttgtttt accacctaat aaatgttctt
    10501 tgataacatt gagataagga atttgaggga aggcaccagc cttgatttca tatccatatt
    10561 ctcgaatata ttcaagtggc attgattttt gtcccttatc ttgatgatag aagcgaatca
    10621 aatcgaatgc cggcaataag taggtttctt gctgagaaga aaagtgaaga aggacaaagc
    10681 agattccttg ttgggcaagg acttgttcca tatgctgaat ctgatgtgga tgaaaatttt
    10741 tcatcggaat cgcacgtttt tgttttgttt ccttgacttc aaagtcgatg taatatccat
    10801 tataaacgcc agaatagtcc gtcgttgaag cttgtcgaaa ataggcttca acaatcttgg
    10861 cacgacttcg ttgtggatag tccacttgta cgatttgaat aggagttggt ttcttatgta
    10921 taacagccaa gccctgagac aaatagtagt cgttggtagc attgatcatc ttttcaaaag
    10981 acattcctcg atttgcgaaa tttttgggtt gagaaagaga tgtttgtctt ttttgtgatg
    11041 aaactttatg tggatagttg accataattc tccttattgg tacaataaca tcactttatt
    11101 ataccataat ttgcacagaa agggttaaaa atggctacag ctttggtttt aggctattct
    11161 gcttttgatt tgggtttatt ttctgataag gatcctcgtc ttaaattgat taaaaaagcg
    11221 atacgtaaag atttagaggc tatggcagca gatggggtgt cttggcttgt gtttacaggg
    11281 agtttgggtt ttgaatattg ggttttagag gttgctcagg agatgaaaac cgaatacggt
    11341 ttccagttgg ccaccatttt tgcttttgaa acccatgggg aaaattggaa tgaaggcaat
    11401 cagatgaaac tgagtcgctt taagcaggta gactttgtca aatatgccta tcctcgctat
    11461 gaacacaagg ggcagttaag agattatcag cagtttttgc tggaaaacac gactagttcc
    11521 tatctttttt atgatgaaga aaatgaaaca aaactagctt atttttacca aaagatgaaa
    11581 aatcaagagg actattttat aaagagatta acatttgatc aattaaatga acttgctgaa
    11641 aatttttccg aaaattgaag ctttgacctt gatttttgtt tgcctttttt tatataataa
    11701 tactagcaag cgagaatgga gagagacatg gcaagtatta ttttttcagc gaaagatatt
    11761 tttgaacaag agtttggacg tgaagtccgt ggctataata aagtagaagt tgacgagttt
    11821 ttagacgatg tcatcaagga ctatgaaacc tatgctgcct tggtcaagtc acttcgtcag
    11881 gaaattgcgg atttgaagga agaattaact cgtaaaccga aaccttcacc agttcaagca
    11941 gaaccccttg aagcggcaat tacaagttct atgacgaatt ttgatatttt gaaacgcctg
    12001 aatagattgg aaaaagaagt ttttggtaaa caaattttag ataactcaga tttttaagta
    12061 gttatttgag atgtgcaatt tttggataat cgcgtgagga gaattgcttc tcatgaggaa
    12121 agtccatgct agcacaggct gtgatgcctg tagtgtttgt gctaggcgaa accataagcc
    12181 tagggacgag aaatcgttac ggcagttgaa atggctaagt ccttggataa gccagagtag
    12241 gcttgaaagt gccacagtga cggagtcttt ctggaaacag agagagtgga acgcggtaaa
    12301 cccctcaagc tagcaaccca aattttggtc ggggcatgga gtacgcggaa acgaacgtag
    12361 tattctgact gctatcagct agagctgtta gtggtagaca gatgattatc gaaggaagtg
    12421 gtcctagtca cttctggaac aaaacatggc ttatagaaaa ttgcatatag gttggggctg
    12481 agaaattttc tcaacctcat tttttaaagt ggacatatag aaaggtcttg caagactgta
    12541 acatgaaaaa agaatttaat ttaattgcaa ctgtggcagc agggcttgag gctgtcgttg
    12601 gtcgtgaagt gcgagagttg ggctacgatt gtcaggttga aaatggacgt gttcgttttc
    12661 aaggagacgt gagagctatt atcgaaacca acctttggct tcgggcagca gatcgtatca
    12721 aaattatcgt aggaacgttc ccagctaaga cttttgaaga gctatttcag ggagttttcg
    12781 ctttagattg ggaaaattat ttaccacttg gagctcggtt cccgatttca aaagctaaat
    12841 gtgttaagtc caaacttcac aatgagccca gtgttcaggc tatttctaag aaagctgttg
    12901 tcaagaaatt gcagaaacac tatgctcgcc cagaaggggt tcctttgatg gagaatggtc
    12961 cagagtttaa gattgaggtc tctattctca aagatgtggc aactgtcatg attgatacga
    13021 ccgggtctag cctctttaaa cgtggttatc gtaccgaaaa aggtggcgct cctatcaagg
    13081 aaaatatggc agcagccatt ttacaacttt ctaactggta tccagacaag cctttgattg
    13141 atccgacctg tggttcgggg actttctgta ttgaggcagt tatgattgct agaaagatgg
    13201 cgccaggtct tcgtcgctct tttgcatttg aggaatggaa ctggatcagc gatcgcttga
    13261 ttcaagaagt gcgcacagaa gcggctaaaa aagtagaacg taagcttgag ttggatatca
    13321 tgggctgtga tattgatgct cgcatggtgg aaattgctaa ggccaatgct caggcagctg
    13381 gtgttgcagg agacattact tttaagcaga tgcgcgtgca gaatttacgt tccgataaaa
    13441 tcaatggagt aatcatttcc aatccgcctt atggtgaacg tttgtcagat gatgcagggg
    13501 tgaccaagct ctatgctgag atggggcaag tatttgcacc gctgaaaact tggagcaaat
    13561 ttatcctgac tagtgatgaa gcttttgaaa gcaagtatgg tagccaagca gataagaagc
    13621 gtaagttata caacggaacc ttgaaagtgg atctatatca atattttggt cagcgtgtca
    13681 aacggcaaga ggtaaaatag aaagggatac tcatgagtaa aaaaagacga aatcgtcata
    13741 aaaaagaagg tcaagaaccg caatttgatt ttgatgaagc aaaagagcta acagttggtc
    13801 aagctattcg taaaaatgaa gaagtggaag caggagtctt gcctgaggat tccattttgg
    13861 acaagtatgt taagcaacac agagatgaaa ttgaggcgga taagtttgcg actcgtcaat
    13921 acaaaaaaga ggagttcgtt gaaactcaga gtctggatga tttaattcaa gagatgcgtg
    13981 aggctgtaga gaagtcagaa gcttcttcgg aggaagttcc atcttctgaa gacatcttac
    14041 tacccttgcc tctggacgat gaggagcaag gcttggatcc tctattgcta gatgatgaaa
    14101 atccaacaga aatgactgaa gaagtggaag aggagcaaaa cctttctcgt ctggatcaag
    14161 aggactcaga aaagaaaagc aaaaaaggct ttattttgac cgttttggcg cttgtatcag
    14221 taattatttg tgtcagtgct tattatgtct accgtcaagt ggctcgttcg actaaggaaa
    14281 ttgaaacttc tcaatcaact acagtcaatc aatcggatgt ggatgatttt aatacacttt
    14341 atgacgcctt ttacacagat agcaataaaa cggctttgaa aaatagccag tttgataaac
    14401 tgagtcaact caagacttta cttgataagc tggaaggtag tcgtgaacat acgcttgcca
    14461 aatctaaata tgatagtcta gtaacgcaaa tcaaggccat tcaagatgtc aatgcacaat
    14521 ttgaaaaacc agctattgtg gatggtgtgt tggataccaa tgccaaagcc aaatcggatg
    14581 ctaaatttac agatattaaa actgggaata cggagcttga taaagtgcta gataaggcta
    14641 tcagtcttgg taagagccag caaacaagta cttctagctc aagttcaagt caaactagca
    14701 gctcaagctc tagtcaagca agctcaaata cgactagtga gccaaaacca agtagttcaa
    14761 atgagactag aagtagtcgc agtgaagtca atatgggtct ctcgagtgca ggggttgctg
    14821 ttcaaagaag tgccagtcgt gttgcctata atcagtctgc tattgatgat agtaataact
    14881 ctgcctggga ttttgcggat ggtgttttgg aacaaattct agcaacttca cgttcacgtg
    14941 gctatatcac tggagaccaa tatatccttg aacgtgtcaa tatcgttaac ggcaatggtt
    15001 attacaacct ctataagcca gatggaacct atctctttac ccttaactgt aagacaggct
    15061 actttgtcgg aaatggcgct ggtcatgcgg atgacttgga ctactaagca gtcgttacaa
    15121 aattctttct tttcaaaagt aaaaatgata aaataaaaca aatgaaacaa gaggagtgtc
    15181 aaatgacaaa agctaacttt ggtgtcgtag gtatggccgt aatgggtcgt aaccttgccc
    15241 ttaatattga atcacgtggt tacacagttg ctatctacaa ccgtagtaaa gaaaaaacgg
    15301 aagatgtgat tgcttgccat cctgaaaaga attttgtacc aagctatgac gttgaaagtt
    15361 ttgtaaactc aatcgaaaaa cctcgtcgta tcatgctgat ggttcaagct ggacctggta
    15421 cagatgctac tatccaagcc cttcttccac accttgacaa aggtgatatc ttgattgacg
    15481 gaggaaacac tttctacaaa gataccatcc gtcgtaatga agaattggca aactcaggta
    15541 tcaactttat cggtactgga gtttctggtg gtgaaaaagg tgcccttgaa ggtccttcta
    15601 tcatgcctgg tggacaaaaa gaggcctacg aattggtttc ggatgttctt gaagaaatct
    15661 cagctaaagc accagaagat ggcaaaccat gtgtgactta tatcggtcct gatggagctg
    15721 gtcactatgt gaaaatggtt cacaacggta tcgagtacgg tgatatgcaa ttgatcgcag
    15781 aaagctatga cttgatgcaa cacttgctag gcctttctgc agaggatatg gctgaaatct
    15841 ttactgagtg gaacaagggt gaattggata gctacttgat cgaaatcaca gctgatatct
    15901 tgagccgtaa agacgatgaa gaccaagatg gaccaatcgt agactacatc cttgatgctg
    15961 caggtaacaa gggaactggt aaatggacga gccaatcatc tcttgacctt ggtgtaccat
    16021 tgtcactgat tactgagtca gtgtttgcac gctacatttc aacttacaaa gaagaacgtg
    16081 tacatgctag caaggtgctt ccaaaaccag ctgccttcaa ctttgaagga gacaaggctg
    16141 aattgattga aaaaatccgt caagcccttt acttctcaaa aatcatttca tacgcacaag
    16201 gatttgctca attgcgtgta gcctctaaag aaaacaactg gaacttgcca tttgcagata
    16261 tcgcatctat ctggcgtgat ggctgtatca tccgttctcg tttcttgcaa aagattacag
    16321 atgcttacaa ccgcgatgca gatcttgcca accttctttt ggacgagtac ttcttggatg
    16381 ttactgctaa gtaccaacaa gcagtacgtg atatcgtagc tcttgcggtt caagctggtg
    16441 tgccagtgcc aactttctca gcagctatta cttactttga tagctaccgt tcagctgacc
    16501 ttccggctaa cttgatccaa gcacaacgtg actactttgg tgctcacact taccaacgta
    16561 aagacaaaga aggaaccttc cactactctt ggtatgacga aaaataagta ggtcagccat
    16621 ggggaaacgg attttattac ttgagaaaga acgaaatcta gctcattttt taagtttgga
    16681 actccagaaa gagcagtatc gggttgatct ggtagaggag gggcaaaaag ccctttccat
    16741 ggctcttcag acagactatg atttgatttt attgaacgtt aatctgggag atatgatggc
    16801 tcaggatttt gcagaaaaat tgagtcgaac taaacctgcc tcagtcatca tgattttaga
    16861 tcattgggaa gacttgcaag aagagctgga agttgttcag cgttttgcag tttcatacat
    16921 ctataagcca gtctttatcg aaaatctggt agcgcgtatt tcggcgatct tccgaggtcg
    16981 ggacttcatt gatcaacact gcagtctgat gaaagttcca aggacctacc gcaatcttag
    17041 gatagatgtt gaacatcaca cggtttatcg tggtgaagag atgattgctc tgacccgccg
    17101 tgagtatgac cttttggcga cacttatggg aagcaagaaa gtattgactc gtgagcaatt
    17161 gttggaaagt gtttggaagt atgaaagtgc gaccgagaca aatatcgtag atgtctatat
    17221 ccgctatcta cggagcaagc ttgatgttaa aggacaaaaa agctacatta aaaccgtgcg
    17281 tggtgttggt tacaccatgc aagaatagaa aagcagttgc agttgtgtaa ctgctttttt
    17341 gaggagtttt tatatattga catgcagtcg ggtctttgct acaatcgttt taggaggata
    17401 tataaatgaa acttttgaaa aaaatgatgc aagttctact agcagtcttt ttctttggtt
    17461 tgctagctac aaatacggta tttgcgaata ccacaggtgg ccgatttgtt gataaggata
    17521 atagaaaata ttatgtaaaa gatgatcata aagcaatcta ttggcataaa atagacggta
    17581 aaacttacta ttttggtgat attggagaga tggttgtcgg ttggcaatac ttagaaattc
    17641 ctggaacagg ttatcgtgat gatttatttg ataatcgtcc agtcttcgaa attggccttc
    17701 aggagaagtg gtactatttt ggacaagatg gtgctttgct agaacaaaca gataaacaag
    17761 tactagaggc aaaaacgtct gaaaatacag gaaaagtata cggtgaacaa tatcctctat
    17821 ctgctgaaaa gagaacttat tattttgata ataattatgc tgtaaagaca ggctggattt
    17881 atgaagacgg caattggtat tatttaaata agctaggaaa ttttggtgat gattcttaca
    17941 atccactacc aattggtgaa gttgctaagg gttggactca agattttcat gttactattg
    18001 acattgatag aagcaaacct gctccatggt actacctaga tccaataact ggtattatgc
    18061 aaacaggttg gcaatatcta ggtaataagt ggtactacct ccgttcctca ggagcaatgg
    18121 ccactggctg gtatcaggaa ggtaccactt ggtattattt agaccagcca aatggcgata
    18181 tgaaaacagg ttggcaaaac cttgggaaca aatggtacta tctccgttcc tcaggagcta
    18241 tggcaactgg ttggtatcaa gatggttcaa cttggtacta cctaaatgca ggtaatggag
    18301 acatgaagac aggttggttc caggtcaatg gcaactggta ctatgcttat agctcaggtg
    18361 ctttggcagt gaatacgacc gtagatggct attctgtcaa ctataatggc gaatgggttc
    18421 ggtaatgaca gggcgcttat aattatatta gaacactcgc ttaatcaaag aagtgacttt
    18481 gttgtacttg acctgacgat tggggagatt aaaagcatgt gaaatctaca catgcttttt
    18541 cgttagttat gagtgttgaa cttgactaac cattgacaaa agataagagc aatcaaaata
    18601 agatacaaaa atatcaatgc taactgcacc gaaacgcccg atgctatgga tagggctaat
    18661 agggagagca gggacggggc gagaatgtct actgtgctca gagcagagcc caccgtcccc
    18721 atcttgtcac tggggatatg ggcaaagaca gctgcttgta ggcgagggct gagaatgccg
    18781 ataatcgtag aactgatgaa actggtcacg aaaattacga tataggcttg acaaaggata
    18841 ctagttatta gagccaatag ggaaatctca cagaaaacca agaggtgcgt gaaacggata
    18901 tttttaaata gattactgct ggtcatattg ccaaggatag ctccacaaga catgatgatg
    18961 gagagcaggg cgaagagttg cccaatgctc agttgtgcta gtgtctgttg gatgaagcgg
    19021 agggcaatca gccgagggga cacatccaag atggcaatca tactggtcgt cagaaacagt
    19081 agtatgacgg tatcttttaa cctcaggagc acagccagcg actgtttaag atgttgaaaa
    19141 tatttcttaa aactcagtgc tgtatgtgac atttcaatcc ttttttcaac ctcatacaag
    19201 gtatgtcgaa taacatacag gcccaaaaag gcaatgacaa aagtcagagt gttgataagg
    19261 gaaatagttt gaatacttat aacattgata aggaatgcgc cagccagatt ggcgacaatg
    19321 tggacaaccc tcatcagaga ctgcctgaac cccatggcat catgcaggtc atccttaatt
    19381 acactgatgt agagggcgtt catcatgtag gcagacaggt agctcaaggt gtcagagatg
    19441 aggttgatga tgacaatcat aatgactgaa actaatgaaa agggcgacaa gacgagaaat
    19501 actgtcaaaa tagcgtagag caggattttg attttggcaa tacagagtgc gtgtttgact
    19561 ctattttctt gaaaatctcc tagaatcccg agaaagagtt gcgatagtag gggaaccatc
    19621 tctgaaatgg aaatcagtaa aatcgctagc ggagcgaaat tgtactgagc cacataattg
    19681 ataatggcga ggtagaaaat actgtcgccg aatgatgaaa aacagctgtt tagcagcaac
    19741 tgtctatata tgttgtttgt ccaaaataat ttcatacatt tctccttatg tctgcacatc
    19801 gaatgggaat ttattattta atagacacca atataccccc atgcttccta ttccgaagtc
    19861 aaacaaatct gctttaccac tatctccttg aaacatcagt aaaccgtgat catctcgttc
    19921 agaacatgct aaaattacct gttggatttt tttcatcagt aatttgtttt gattgtagac
    19981 agtggtttgt agtaagctag ttattccgtg gcaatatcct gtcatcattt ttagatgatg
    20041 gttaaaaaca aattcttgat atttactaat aatatctttg tttccgtcac aatcgtacat
    20101 acaaagatat aagattattc cggaaattcc tttgcaccaa gaaagttgtg aatttcctaa
    20161 attttcagta ttagaagtaa ctttttctaa taattttttt aattcagtat ggaatgtatg
    20221 gagatcatta tagaacatag aaggttctaa gactttagaa taggcaaata aagcctatgc
    20281 aattccacaa tatccatgag caaagctata gtctaaaatg ctttctttgg ctatatttct
    20341 ttgtaggaaa taatgctaaa tttcccctat actattgtgg atgaaaattt taagagtatt
    20401 gtcattggta aattgatagt attttattaa tgataatagg acaccagatt ttcccaatgc
    20461 aaagtctatt tcctctagag tatcataatt ctctattaat ttcttagcgg tgacgtttgc
    20521 taattgtaga tagtattgat ttttactgat agaaaataaa tgtaggagta accatagata
    20581 acctgcatca ccaaatagca gtgagtattg atattcatat gtgtgtgtcg ttctaattgt
    20641 cactgccttc ttgatgatag agagtatttt agaatccagt tctggatggt acaatttatt
    20701 tagaggaaac agtagtccag ctattccgtg ttggaatgat aaattactta cgaaatttcc
    20761 aaattctgtt gaagaagtga tccttccttc agaatttaca gttttatcta gtaaaaatcg
    20821 tagaacactg ttacattctg ttttgtaatc atatttatgt tcctctattt ttttaatttt
    20881 gactaaatta tttgtttgac ttaaatttag tagatattgt aaccaaaata gttgctca
//
LOCUS       DAVLWA010000027        20834 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLWA010000027 DAVLWA010000000
VERSION     DAVLWA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.027"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(141..755)
                     /locus_tag="VYN80_000988"
     CDS             complement(141..755)
                     /locus_tag="VYN80_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1540919.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1571..1840)
                     /gene="rpsO"
                     /locus_tag="VYN80_000989"
     CDS             complement(1571..1840)
                     /gene="rpsO"
                     /locus_tag="VYN80_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1540920.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1981..2610)
                     /locus_tag="VYN80_000990"
     CDS             complement(1981..2610)
                     /locus_tag="VYN80_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1540921.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKEHISLLEK"
     gene            2849..3049
                     /locus_tag="VYN80_000991"
     CDS             2849..3049
                     /locus_tag="VYN80_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540922.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3480..3620
                     /locus_tag="VYN80_000992"
     CDS             3480..3620
                     /locus_tag="VYN80_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1540923.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3627..3803
                     /locus_tag="VYN80_000993"
     CDS             3627..3803
                     /locus_tag="VYN80_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1540924.1"
                     /translation="MTKQGRYYDETSYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            3861..5804
                     /gene="thrS"
                     /locus_tag="VYN80_000994"
     CDS             3861..5804
                     /gene="thrS"
                     /locus_tag="VYN80_000994"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1540925.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LEHYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5853..6827)
                     /locus_tag="VYN80_000995"
     CDS             complement(5853..6827)
                     /locus_tag="VYN80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1540926.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6820..7497)
                     /locus_tag="VYN80_000996"
     CDS             complement(6820..7497)
                     /locus_tag="VYN80_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540927.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7653..8726
                     /locus_tag="VYN80_000997"
     CDS             7653..8726
                     /locus_tag="VYN80_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1540928.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDAELQLYFDLFFGTILDAGISSINDLTSLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8817..9119)
                     /locus_tag="VYN80_000998"
     CDS             complement(8817..9119)
                     /locus_tag="VYN80_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540929.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLDFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9188..9625)
                     /locus_tag="VYN80_000999"
     CDS             complement(9188..9625)
                     /locus_tag="VYN80_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1540930.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9701..10543)
                     /locus_tag="VYN80_001000"
     CDS             complement(9701..10543)
                     /locus_tag="VYN80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1540931.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMNFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10655..11305
                     /locus_tag="VYN80_001001"
     CDS             10655..11305
                     /locus_tag="VYN80_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1540932.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11493..12158
                     /locus_tag="VYN80_001002"
     CDS             11493..12158
                     /locus_tag="VYN80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1540933.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            12260..12697
                     /locus_tag="VYN80_001003"
     CDS             12260..12697
                     /locus_tag="VYN80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540934.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12792..13091)
                     /locus_tag="VYN80_001004"
     CDS             complement(12792..13091)
                     /locus_tag="VYN80_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540935.1"
                     /translation="MNRDVLEFLRTETAEKISLYISEANRLEGDVTLLAPSSQDLEDI
                     KNAMLSNSNLGLKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13272..13634)
                     /locus_tag="VYN80_001005"
     CDS             complement(13272..13634)
                     /locus_tag="VYN80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540936.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEEDVLILLSELQNFFLDVFQAKRIHRA
                     FCVRLCLKAQFLLSNNDS"
     gene            13993..14408
                     /locus_tag="VYN80_001006"
                     /pseudo
     CDS             13993..14408
                     /locus_tag="VYN80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14447..14890)
                     /locus_tag="VYN80_001007"
     CDS             complement(14447..14890)
                     /locus_tag="VYN80_001007"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1540937.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTDIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(14920..17142)
                     /locus_tag="VYN80_001008"
     CDS             complement(14920..17142)
                     /locus_tag="VYN80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1540938.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALIYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17553..17795
                     /locus_tag="VYN80_001009"
     CDS             17553..17795
                     /locus_tag="VYN80_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540939.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            18135..18764
                     /locus_tag="VYN80_001010"
     CDS             18135..18764
                     /locus_tag="VYN80_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1540940.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(18887..20779)
                     /gene="pepO"
                     /locus_tag="VYN80_001011"
     CDS             complement(18887..20779)
                     /gene="pepO"
                     /locus_tag="VYN80_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1540941.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         6137 a         4675 c         3789 g         6233 t
ORIGIN      
        1 taagaatctc ctaatcaaat caaaagccac tctacaatag agtaacttta agattgtcca
       61 tttatactca atgaaaaagc cctctgaaaa tcagagagct gatttgagct cgggctaaaa
      121 tcctagtgaa acaaaaaaat ctacacgatc agaaaagtct ctatcgtgcc attttcatac
      181 atgatgtata gtccaagtag aatgaatact aagggcacaa tgattcgctc gtatttttca
      241 attgtctcga atattaacgg aatagaggat aacaccctac taatctcgca aaagataatt
      301 atgccgatta caaacacaag caaggccacg agggtctgtg accaatctaa cgaagcaaaa
      361 taaggtatat agatacctaa attatctccg ccagacgcaa ttgtcagcaa tgtaactgtc
      421 caaaacagtt gatttgcctt gctttgttct aatctttcaa taatttcttc ctcttcttct
      481 tccgcatctt ctccaacaat tgcaaagcga atccctaaat agatagggat taaaccaagc
      541 aatccaacca tccattcttc aggcacgaaa ttaacgacat aagcagcaac taaactcgcc
      601 cctacaagta agcctgtgcc tagatattgc cccgcataaa tatgccattt ctgtttattc
      661 tgtgatagct gtgcaaataa aataattaaa ataattaaat aatcgatact ggtggaaata
      721 taaacaccaa tagcagatat gattgtctgt cccataaaaa aaacctcctg tattagtcag
      781 taataaatca gcagatttta ggagagcaca gacacattaa tttagctatc gctagtgggt
      841 aatgtcgaac gtaggaaagc attttactcc tcctcaaaac gtagttacaa agtaatttct
      901 aactggaatt cagtgactac ttccatgtaa agtataacat actatacttt actttgtaaa
      961 gagtgggctc ttctaggggg cttgcataca gctactcggc ttgaaaagcc gagtaactgc
     1021 gcacctttat ggtgtaatta gctgataccg tttgaggttt ttgtagtctc caaaattgtt
     1081 accgataaaa cctcaataaa aaagccctct gaagtcagag agctgatttg agcccgggct
     1141 aaaatcctag tgaaaaagat gaaactcctt gtgctcatcg aacactgtgt catttcccta
     1201 ttttcatacg gatttttgac gcccttagca tcatgattct tgctagataa atcgtttata
     1261 gactaggcga cgaggcgaag atcgtacgaa actttcagtg aaacaaaaaa aatctccccg
     1321 aagggagaaa agctggttta ttttacctta cagtgctagg aaccgtaacc aaagattata
     1381 cttgagtata tcgaggtaag gtgacaacgc aaaaaagcta cccaatttag agtagcttca
     1441 ttatcaagat atgctcaatt gaacacggcc taagcacttg gcaaaaaaga taaaatctcc
     1501 tagaaactga agtttcttcg tcaattttcc tatttttgct ttgtgctttt gacagcattt
     1561 gtatcttgaa ttagcgacga agtcctagag agttgatcaa ctcacggtaa cggttaacgt
     1621 cgtttttacg caagtatgca agcaagttac gacggcgacc gatttttttc atcaatccac
     1681 ggtaagtagc gtggtctttt ttgtgttgtt tgatgtgttc gttaaggtgg ttgatttccc
     1741 aagtaaggac agcaacttga acctctactg aacctgtatc accttcgtga cgtgcatatt
     1801 gtgcaatgat ttcatttttt ttctcttttg agattgccat gatgtttctc ctttttattt
     1861 ggcttcatcc gagtgacagg ttggcatgcc tgtaaccaag aagaagttat ttgtctttac
     1921 gacaatccct attttaccaa gatttaaatt ttctgtcaaa cattaatact ctcatagtta
     1981 ttatttttca agtaaagaga tatgttcttt tatatgatga cctttttcaa gttcatctac
     2041 caatccaata gcataatctg cataactaat ctggcttatt cctttttcat tagtagtaaa
     2101 aatttctcct gctagaatgt agtttcctgt tttttcacca tcgggaataa aatctacagc
     2161 aggactgaca aaagtccagt tgagattatt ttttgtacga agaagatcca attcatccgc
     2221 ttgatctttt gctagtgact taaattcttc aggaaaatca gacgtatcta aaagtcgtgt
     2281 cgtttttgtt tcatctatat aaagacttcc tgctccccct acaactagaa aacgtgtctg
     2341 tgttccagca agaagctgat taaatagttc gattgatttg ctgtggagcg gtagcgtatc
     2401 tggtgtataa gcaccaaacg ctgaaataac agcatcaaat ccagtaagat catcttttgt
     2461 caactcaaat aaatcttttt taataataga ctcagcttga cttttgtttt cagaacgaac
     2521 aatagccgtt acttcatgtc ctcgtttgac tgcttcttca acaattgctt tccccgcttg
     2581 tccatttgct gcaataactg ctagtttcat tttttatacc tctattgttg taatcatttt
     2641 agttacaaca attgtgacac tcttaataat caatatcaat agtcttgctt aattattatc
     2701 aaaatatttc taccaagaaa actaactatg attctagtga aaagaaaaat cttctttgtc
     2761 aacaaattta ctttcttgtt ttaaacatgc tataataatc atagcaagag atctaagttg
     2821 tctgtttttt taaaacgagg tgattatcat gcgtagattc tattcccatc tcccctacta
     2881 tctggtcata ttattctttt attggccact ttatgagttg ttcttgctag ttgtttctga
     2941 cccccttaca ctcaagggac tctatataaa caatcttctc ttctttacac ctatggtaat
     3001 cttgattgta tcgttactct atagctaccg tttccgtttc tcactttgat ggttagttgg
     3061 taacggactg ctcttttact ttactatcat aacctttggt gagtttatac taatttactt
     3121 gctaatctat gaaacagttg ctctggtggg catggattct ggtattagca tcaagcatat
     3181 tctacaaaaa atgaaaaaca aaaaactttc acaaaatcct tgaaaaatct cacaatcatg
     3241 ctataataat ccatagagac aagtcactta gtccctttct actagagagt gcgtggttgc
     3301 tggaaacgca taggaagtct aaactgatac tactcttgag ttttttatga aaacataaaa
     3361 cggtggccac gttagagccg atcagaggtg tccctctctt ttgaggtaca taaatgaagg
     3421 tggaaccacg ttgcgacgtc ctttcgagga tgtcgcattt ttttattagg atactaatta
     3481 tggagttgca agaattagtg gagcgcagtt gggcaatccg acaagcttat cacgaactgg
     3541 aagttaagca tcatgatttc aagtggacgg tagaagaaga cctcttggct ttatctaatg
     3601 atattggaaa tttccaatga ctggtgatga caaagcaagg acgctactat gatgaaacat
     3661 cctacacact ggaacaaaaa ctttcagaaa atatctggtg gctattagaa ctttctcaac
     3721 gtttggatat agacattctg acggaaatgg aaaacttcct ctctgataaa gaaaagcaat
     3781 tgaacgttag gactcggaag tagtctgctg ataaaaaatc aatgcttaga aactatgaaa
     3841 taataaaaaa ggagaacatc atgattaaca ttactttccc agatggcgct gttcgtgaat
     3901 tcgaatctgg cgtaacaact tttgaaattg cccaatctat cagcaattcc ctagctaaaa
     3961 aagccttggc tggtaaattc aacggcaaac tcatcgacac tactcgcgct atcactgaag
     4021 atggaagcat cgaaattgtg acacctgatc acgaagatgc ccttccaatc ttgcgtcact
     4081 cagcagctca cttgttcgcc caagcagctc gtcgtctttt cccagacatt cacttgggag
     4141 ttggtccagc catcgaagat ggtttctact acgatactga caacacagct ggtcaaatct
     4201 ctaacgaaga ccttcctcgt atcgaagaag aaatgcaaaa aatcgtcaaa gaaaacttcc
     4261 catctattcg tgaagaagtg actaaagacg aggcacgtga aatcttcaaa aatgaccctt
     4321 acaagttgga attgattgaa gaacactcag aagacgaagg cggtttgact atctatcgtc
     4381 agggtgaata tgtagacctc tgccgtggac ctcacgttcc atcaacaggt cgtatccaaa
     4441 tcttccacct tctccatgta gctggtgcgt actggcgtgg aaacagcgac aacgctatga
     4501 tgcaacgtat ctacggtaca gcttggtttg acaagaaaga cttgaaaaac taccttcaaa
     4561 tgcgtgaaga agctaaggaa cgtgaccacc gtaaacttgg taaagagctt gacctcttta
     4621 tgatttcaca agaagtggga caaggtttgc cattctggtt gccaaatggt gcgactatcc
     4681 gtcgtgaatt ggaacactac atcgtaaaca aagagttggc ttctggctac caacacgtct
     4741 acactccacc acttgcttct gttgagcttt acaagacttc tggtcactgg gatcattacc
     4801 aagaagacat gttcccaacc atggacatgg gtgacgggga agaatttgtc cttcgtccaa
     4861 tgaactgtcc gcaccacatc caagttttca aacaccatgt tcactcttac cgtgaattgc
     4921 caatccgtat cgctgaaatc ggtatgatgc accgttacga aaaatctggt gccctcactg
     4981 gccttcaacg tgtacgtgaa atgtcactca acgacggtca cctattcgtt actccagaac
     5041 aaatccaaga agaattccaa cgtgcccttc agttgattat cgatgtttat gaagacttca
     5101 acttgactga ctaccgcttc cgcctctctc ttcgtgaccc tcaagatact cataagtact
     5161 ttgataacga tgagatgtgg gaaaatgccc aaaccatgct tcgtgcagct cttgatgaaa
     5221 tgggcgtgga ctactttgaa gccgaaggtg aagcagcctt ctacggacca aaattggata
     5281 tccagattaa aactgccctt ggaaaagaag aaaccctttc tactatccaa cttgatttct
     5341 tgttgccaga acgcttcgac ctcaaataca tcggagctga tggcgaagat caccgtccag
     5401 tcatgatcca ccgtggggtt atctcaacta tggaacgctt cacagctatc ttgattgaga
     5461 actacaaggg ggccttccca acatggctgg caccacacca agtaaccctc atcccagtat
     5521 ctaacgaaaa acacgtggac tacgcttggg aagtggccaa gaaactccgt gaccgcggtg
     5581 tccgtgcaga cgtagatgag cgcaatgaaa aaatgcagtt caagatccgt gcttcacaaa
     5641 ccagcaagat tccttaccaa ttaattgttg gagacaaaga aatggaagac gaaacagtca
     5701 acgttcgtcg ctacggccaa aaagaaacac aaactgtctc agttgataat tttgttcaag
     5761 ctatcctagc tgatatcgcc aacaaatcac gcgttgagaa ataagagtct agcataaaag
     5821 cctccaatct ggaggctttt tctcatctat ttttactcaa ggactaagtt cacttgagca
     5881 aactgaatcc gcactgtcgt tccttttccg acctcagact cgatacgaat ctggtgcccc
     5941 agttcttcag aaattttctt agatagataa aggccaagtc cagaggactg ctgggtcaaa
     6001 cggccattgt atcctgaaaa gccacgttca aatactcgga ggacatcact gttttttatc
     6061 ccgattcccg tatctttgat acaaagctct tggtcatcca tataaatctc cagaccacct
     6121 tccttggtgt acttgagact gtttgagatg atttgctcaa taaccactag cagccacttt
     6181 ttatccgtca cgatttcttt atcaaggtca tgtagattga catttaagcc tttttgaata
     6241 aagaaaagag catatttacg aattatttcc ttgaccaagt cctcaatttg aacctgcttt
     6301 aagagcaaat catcatggaa actttctaaa cgcaggtact gtaaaactag gttggtatag
     6361 gagtcgattt tgaaaatttc ctgttctagc tgctgcttca gttggcggtc gaccacttct
     6421 gcaactaaga gttgactggc tgcaatgggg gtctttatct gatggaccca caaggtatag
     6481 taatccagca aatccgtcag ttttctttct gcttttgacc tctgctgata gagttccatc
     6541 tcacgcgctt ctaatttttc tgctaaagct atttccaaag gagacttggc ttccctctct
     6601 ccatagagaa gttcctggcg atagacctgc gtttccacca atatgtccca agtgaaaaat
     6661 aagatggtta caaagcaaca caagaagaaa aagtagagga agtaaattcc tagactggca
     6721 aataaaaact gaaagagtaa gacaagaaat gccaaagaaa gcagatagat aaaaagacga
     6781 ctacgggagc gcagataggc tagaaaaaat tgtttccaat caagcatgct tcaatccgta
     6841 ccctattcct ttcttggtct cgataaatcc taccaatccc tgctcctcca actttttacg
     6901 caaacgagcc acattgacag agagggtatt atcatcaatg aaaaagtcac tgttccaaag
     6961 ttcccgcatc aggtcgtcac gtgctacgat gttgcctgca tgctcaaata acacgcgtaa
     7021 aatctggaat tcattcttgg tcaaattcaa gacttgccct tgataatgta aatccatgga
     7081 tttggtattg aggataacac cagcatattc cagcaaactc tcatcacgcc caaactcata
     7141 ggaacgacgc aacaagccct gaaccttagc taaaagaacc tgctggtcaa aaggcttggt
     7201 cacaaagtca tccgccccca tattgattgc catgacaata tccatagcct ggtctctcga
     7261 agaaagaaac atgataggta ccttggaaat cttgcggatt tcctgacacc agtgataacc
     7321 attaaacaag ggcaaaccaa tatccatgag gaccagatga ggttccgact gaacaaatag
     7381 actcaaaact tccataaagt cttctaccag gaccacttca aatccccatt cagagagcat
     7441 tttcccgacc tgttgacgaa tgacctgatc atcttctatt aataaaatct tgtgcatgcg
     7501 cttctccttt tctattatta taacagattt ttccatgcca gatggtctga aactgaattt
     7561 gaaatagcct gtttttagcc agtacaaaca ggcaatgcta ctagctaatt tgagggaaat
     7621 ttgctaagat aaataaaaag aaaggagctc ttatggccaa tatttttgac tatctgaaag
     7681 atgtcgcata tgattcttat tacgaccttc ccttgaatga gttagacatt ctaaccttaa
     7741 tagaaatcac ctacctctcc tttgataatc tggtctccac acttcctcaa cgtcttttag
     7801 atctagcacc tcaggttcca agagagccca ccatgcttac tagcaaaaat cgccttcaat
     7861 tattagatga attggctcaa cacaagcgct tcaaaaattg caaactctcc cattttatca
     7921 acgacatcga ccctgaactg caaaagcaat ttgcggctat gacttatcgt gtcagcctcg
     7981 atacctatct gattgtcttt cgtgggacag atgacagtat cattggctgg aaggaagatt
     8041 tccacctgac ctatatgaag gaaattcctg ctcaaaagca cgcccttcgc tatttaaaga
     8101 acttttttgc ccatcatcct aagcaaaagg ttattctggc tgggcattcc aagggaggaa
     8161 atctcgctat ctatgctgct agccaaattg agcaaagttt gcaaaatcag atcacagcag
     8221 tttatacatt tgatgcacct ggtctccatc aagaattgac acagactgcg ggttatcaaa
     8281 ggataatgga tagaagcaag atattcattc cacaaggttc cattatcggt atgatgctgg
     8341 aaattcctgc tcaccaaatc atcgttcaga gtactgccct gggtggcatc gcccagcacg
     8401 atacctttag ttggcagatt gaggacaagc acttcgtcca actggataag accaacagtg
     8461 atagccagca agtagacaca acctttaaag aatgggtggc cacagtccct gacgcagaac
     8521 ttcagctcta cttcgacctc ttctttggca ctattcttga tgctggtatt agctctatca
     8581 atgacttgac ttccttaaag gcgcttgaat acattcatca tctctttgtc caagctcaat
     8641 ccctcactcc agaagaaaga gaaaccttgg gtcgccttac ccagttattg attgatactc
     8701 gttaccaggc atggaaaaat agataatact cttgaaaatt aaatgtatac aaaacaaaag
     8761 acctagaata catactttca tgtgcattct aagtcttttt aaatagaatc taatagtcaa
     8821 taaaaatcaa agagcattga gagataatgg ggcttggaac gtccctctcg cttcaacaaa
     8881 atgaccccat tatagattaa aaagatgcca cttagaaaaa gcaaaaaagg aagtaagaca
     8941 aaggcaaata cataaaaatc taactgaaca ttctcgtatc catttttata aaaaaggtag
     9001 gatagataaa aataacttga aatgagggat aataaaaata atactggatt ccacaaactt
     9061 ctattatcct tccaaaatga cactataaag gctaatacaa ttcctataac gagatacatt
     9121 tcttactcct ttaatagcta cattttatca taattatcca aagaaaaaaa gagggcattt
     9181 atccctctta atccttcatc tgactctctg catcggccac gactttttct agactggtct
     9241 gaccaagttc tgcctccata gtcaactgaa ttctctccaa tttttgatcc aaaacatcat
     9301 gaatatgagc tcctacaggg caatttggat tcggattgtc atggaaactg aagagttgac
     9361 ctgtcttacc aagacattcg accgcctgat aaacatctaa aagactaata tccttaaggt
     9421 ccttgacaat ctctgttccg cccgttccac gcgctactga aatcagctct gccttcttca
     9481 actgggacaa gatctttctg ataatgacag gattgacccc gacactagca gccagaaaat
     9541 cactggtcac cttgctttcc ttcccctcga gggcaatgat tatcagcata tgagtcgcaa
     9601 tggtaaatct acttggaatt tgcatcctct tctccttttt acgaggctac cctgcctcta
     9661 ctcttctttt tctattatta tacccttttt agttgtaatg tcaatcgtta ccacttttca
     9721 accagtcgtc taactcccga tcgcaaccct ctttctgagc caattctctc aaaaattcct
     9781 gatgatgagt atggtggatc ccattgacca gactttcata gtaaacctca aaatagggaa
     9841 gtctcaggtc tttagccagc tgcaattcag ctgctacatc gtagtctacc cgtcggaagt
     9901 tcatatctac caggcccttg tcatcaaact ccaaaatcat atactgggcc cgcaagtcct
     9961 tccgtagctg agcgtccaaa aagaaaggtt ggccaatcga acccggattg acaatcaatt
    10021 gcccaccagt cccgtaacga agcaactgct ggtgaatatg tccataaaca gcaatatcac
    10081 agggaggatg agtcaccaag cggtcaaaat cctcttgttt gccagtatga atcaactctc
    10141 gcccccagtt cttatcaggc agatgatgac taattcccac cgtcaaatcc ccaaactgac
    10201 gatgaatttg aagaggttga ttgtggagca cttcaatttc ttctagggaa atttcctcta
    10261 aaacatactg gcactggcgc aagagatagc gttgactggg gcgagtacta tccaattcct
    10321 tacggacacc atgccaaaga ctgtcttccc agtttcccaa aactctagcc gtaatcggta
    10381 gttgatccaa caagtccaaa atccttctac gtcctgtccc tggcatgaga atatctccca
    10441 aaagccagta ttcatccact cctagctgcc gagcatctgc caaaacagcc tccaaggcgg
    10501 tggtatttcc atgaatatct gaaagaagag ctattttcgt catatccatc tcctcgtttt
    10561 ttctcttgca ataagtataa cataaaaagt cacagctaga gaaatctagc tttttttgat
    10621 atactagata aagatattag acaagaggaa acgaatgacc ccaaacaaag aagactatct
    10681 aaaatgtatt tatgaaattg gcatagacct gcataagatt accaacaagg aaattgcggc
    10741 tcgcatgcaa gtctctcccc ctgccgtaac tgaaatgatc aaacgaatga aaagtgaaaa
    10801 cctcatccta aaggacaagg aatgtggcta tctactgact gacctcggtc tcaaactagt
    10861 ctctgagctc tatcgtaagc accgcttgat tgaagttttt ctagttcatc atttagacta
    10921 tacaagtgac cagattcacg aggaagctga ggtcttggaa cacactgtct ctgacctgtt
    10981 cgtggaaaga ctagataaac tgctaggttt ccctaaaacc tgcccccacg gaggaactat
    11041 tcctgccaag ggagaactcc tcgttgaaat caataacctc ccactagctg atatcaagga
    11101 agctggcgcc taccgcctga ctcgggtgca cgataatttt gacattctcc attatctgga
    11161 caagcactca cttcacatcg gtgaccagct ccaagtcaag cagtttgatg gcttcagcaa
    11221 taccttcact atcctcagta acgacgagga tttacaagtg aatatggaca ttgcaaaaca
    11281 actctatgtc gagaaaatca actaatttct caagtcccct accaaccctg aaagttttat
    11341 tttcagggtt ttatttctat cattttattt tactttcatt tggtgggaga gtatttgaaa
    11401 aaaagaaagg tagtttaacc tatgaaaaaa tcactaaaaa tttttgctac atctaaatag
    11461 tttgacctct tcggggttgc tttggtcgtt ggattgcgat tgcatctggt tacctcaact
    11521 cacgcctcga taaatttgta gactggggac catggacagc tcttgtaccc tttggattga
    11581 tttccgtaac caacgttggg atttccatgt tgtccactcg tttcacgggg aaattaagca
    11641 aatggggaaa ttactttggt attgttaata ccattttgtc cggtgctatt gattatatcc
    11701 ttggaaataa ggcggccatc attacctatc ccgtcacctt cctcatttat acctttgcta
    11761 taaagaaatg gaaagcttcg caagaaggca gacccaacca aatgagccaa aaacaggtaa
    11821 aattggcggc catcatcatt tccatcatcg ccttcctctt tgcctttgtg accaactata
    11881 tcggatatga gggcaagatg aatctccttg cctacgtaac aactattgcc tttgcactgt
    11941 ccctcattgc caatgctttg aatgcattga aactaacaac tcagtggggc ttttggttga
    12001 tttacaattt cgttcagctg acaaaagctg gtattcaagg aaacttcgcc aatatcggta
    12061 aatacatctt ttatatcctc aatgcaatcg gagctttatt tgtctggaat gatgaagaag
    12121 ttagatacat agaaggaaac tcaaataaag aatactagcc aacttaagag aatctccctt
    12181 ctgctatttt tatcatttta gttgtaactc ccattctttt ggagtagaat gaagttagat
    12241 aaaagaggaa ggtcttgtca tgaaaaaact ctatagaatc cattttatag caattgcagt
    12301 tattgatttg ctactatttg cattttttat cactagactc gagacatctt ttgagtggct
    12361 cttgctctct ggtttaattt tcttccttgc tcaaggactc ctgctatttc tcttggtcgt
    12421 ccgactcaaa catcaattcg ctgagattta tcctcaaatc aataaaaaga ttcgcttcta
    12481 ctatttaggg gttctcacca ttgattttct attttttgtt ctcttagcct tcattagttc
    12541 tcagcgtttt tcatctctta tgccaattat cactgcttgc cattctactt tttattatat
    12601 gacagctgac tacctaggag aaaactatcc agacttttac gacaaacaca tctctaacac
    12661 gtatactatt ttatcgattt cttctttttt agtataagac agctcgttct ccagaaatat
    12721 tcattaactt tcatttttat acttttcctt tcgattttca aaaacaaggg gagggattaa
    12781 cctcccttat attagacata acttttagga tcacaattgt atgttccttt cgaaatatcc
    12841 ccaaaaatcg tagcccctgt taaataatga tttcttgtag aagcatatgc tattttcttc
    12901 atcacatcca gtctcgcaac ttttaatccg agattagaat tggaaagcat agcattttta
    12961 atatcttcca aatcttgaga actaggtgcg agtagagtta catcgccctc caatctatta
    13021 gcctcgctta tataaagact aattttttca gcggtctctg ttctcagaaa ctctaataca
    13081 tctctattca tttttttacc tcgtattttt tctaattatt gattttttct aaaaacaaaa
    13141 tcaattcaat ggtgaatctc tagctttcat cttcattcgt tcctctttcc atcctaaata
    13201 aagtagtgtt tttatcccgt aataggtttt attttagcgg agcattacac tccgcattaa
    13261 tattgatagt attaagaatc attattactt aataaaaatt gtgctttcaa gcataggcgt
    13321 acacaaaatg ccctatgtat acgtttcgct tggaagacat ctaagaaaaa attttgtagt
    13381 tcagacagta agattaatac atcttcctct aacataagtg atgtctggta ctcccaatta
    13441 ttgctctgtt ttaacgatga aaagggtata gctgtctctt ttaattttat ccaatctata
    13501 cgttcttttt taggaacgta ttctctaaca gcttgactaa caatactgga atagcttact
    13561 tcatagccta atttattgta aacatcatct ttaagcttat ctaatatctg tctggattca
    13621 gacgctaaac gcattctaac attctctttt tgcacattat cacacctttc taaggtggtt
    13681 tttttatccc gtaaattaag tatatcacat ttagaaaaaa aatcaagaat taagttaaaa
    13741 aaaatttcca caatctaaaa atttatcatt ttatgacaaa catactatcg ttaaatgctg
    13801 atttaatcga aattttgagt tataaactcc tcagtttcga actcaaaatt agccgtgcag
    13861 gataggatac ccttcataaa cttatataac tactttaata ccttaaaaag ctaataaaat
    13921 aaggaatatc taagcaatat acattctaaa atacctgtag gggagtgtct ctaaggaaaa
    13981 ggaggtttta gcatgaaaaa aatcatcttc atcaaaacca ttcaactcct tgtcattgat
    14041 ggaatcatgc tggcattttt gacatttaaa aaggggggct tacttgggac tggattttga
    14101 tttatagcgg ttggctcatt ttctttcatc ctgtgctatt gacctatctt tcaaaccaac
    14161 tttgtgacca ctttagtcag ctctattccc agattagacc gagattctgg cgttttgctt
    14221 tacaaattct cctatgggat agcctgatga ttctctcctt ggtgtcttta agtgatattc
    14281 cacttttcct tcagggaact ctcctcatcc taggacatct catcccttcc tatcgcatct
    14341 gccaaagcct gaaaagagac ttcccccaag catatcaaga accgatttct ttttggagta
    14401 ttttatgata gatgagaaag accaagccga ctgggcttgg tcttttttat ctctttttag
    14461 tatctaggat aatggtaacg ggtccgttat taaccagctc aacctgcata tctgctccaa
    14521 agatatctgt ctgaacgggc acttcttgcg ctaatttttg attgaaagca tcatagaagt
    14581 ctgatgccat atcaggttta gctgcccctg taaaggctgg acgattgcct ctcttagtat
    14641 ccgcaaagag ggtaaactga gaaatagaga ggatttctcc ttcaatatct ttgacagaca
    14701 ggttcatctt gccttctgcg tctgaaaaaa tccgcatatt gaccagtttt ctcacagcat
    14761 agtccaaatc ttcctcttgg tcctctggtc caacaccaac tagcaataaa agtccctgat
    14821 tgatttttcc ctgaatctgg ccttctatac tcacttgggc ttttttaacc cgttggataa
    14881 tgattttcat aatagccttt ctagtaagag ctaggacaac tagccgttgg tccgtttgac
    14941 agagtaaact tctggcacac tcttaatttt atcgacaacc gtggtcagtg tagagaggtt
    15001 ggcaataccg aaggacacat ggatattagc aaacttcata tccttggttg gttgggcatt
    15061 gaccgttgaa atattcttgg ttgtatttga aagaacttgc agtacatcgt tcaacagtcc
    15121 tgtacggttg agaccgtaga tatcgatatg ggccatatac tccttatttg agctagagta
    15181 ctggtcttcc cattccacat caaggagacg ttgctcgtag ttttcttggg cacgcaggtt
    15241 catacagtcc acacggtgaa tagccacacc acgacccttg gtaatgtagc caacaatatc
    15301 gtcaccaggc acggggttac aacacttagc aatccgcact aggagaccag aagcaccttc
    15361 aataaccact cccccctcat gcttgacctt gagagtttct ttattttcaa ccttgacctc
    15421 gccacctttg acaagctcct ctgcctcagc cttggccttg gcacgctctt cctcacggcg
    15481 ttccttttca gtcagacggt taaagacggt aatcgcaccg atttccccaa aaccaatggc
    15541 cgcaaagagg gagtcttctg tcttgtaact ggtcttttgc agaacttgat ccatgtggcg
    15601 cttgtccata aatttatttg ccacatagcc attttcttgg aactgagcca tcagcatctc
    15661 acgacccttg ttgacagaca attccttatc ttggttttta aagaactggc gaatcttatt
    15721 gcgcgccttg ctagtcttga ccatattgag ccagtcacgg ctaggtccaa aggagttcgg
    15781 gttggcgata atttcaacct gatcccctgt ctttaacttg gttgtcagtg gaaccatgcg
    15841 gccattgacc ttggcaccag ttgctttttc accgaccttg gtatggattt cgtaggcaaa
    15901 atcaatcggt cctgaatctt tgggaaggga acggacagct ccatctgggg taaaaacgta
    15961 aatctcctca gccagatagt tttccttaac agagtccaca aattccttag catcatcagc
    16021 ctggtcttgg agctccatca tctccttgat ccagttcatt ccaatagctg attccttgct
    16081 gttaacttgc ccctttatac ctttcttata agcccagtga gccgcaaccc cgtactcagc
    16141 cacctcgtgc atttccttgg ttcgaatctg gaattcaatc ggcccttttg gtccataaac
    16201 agtcgtatgg atagactgat aaccattggc cttgcggttg gcaatatagt ctttgaagcg
    16261 acctggcatc ggtttccaaa attcatgcac gtaaccaagc atggcataaa catcactttg
    16321 ggtatctaaa atacaacgaa tagcaatcag atcatagatt tcctcaaacc gttttctctt
    16381 gtcctgcatt ttgcggaaaa ttgagtaaat atgcttggga cgaccataaa tcttcccttt
    16441 caagtgacgt tctgtcgtat actcctctaa ttttgtgact acctcatcca ccaaggcctc
    16501 acgctccctg cgcttttcct tcatcatatg ggtaatcttg taaaactccg ttggattgag
    16561 ataacggaaa gacaagtctt ctaattccca tttgacactg gaaatcccca aacgatgggc
    16621 aagtggggca tagatttcca tggtttcttt ggaaatacgc tcctgcttgt cttttcgaag
    16681 atgtttcagg gtccgcatat tgtgcaagcg gtcagacagt ttgaccaaaa taacgcggat
    16741 gtcctcagac atggccatga gcatcttgcg atgattttcc gctaattgct cctcgatcga
    16801 tttgtactcg accttgccaa gcttggtaac tccgtcaaca atcacccgca catcaggacc
    16861 aaactctctt tccaaatcgt ccaaggtcgc atctgtatct tccaccacat catgcaagaa
    16921 tccacaagct actgttacag catccagctt tagcttagct aaaatacctg ccacttggat
    16981 agggtgaatg atataaggct cgcctgattt gcgatattga ccactgtggc attcaacagc
    17041 atagatcaag gccttatgga caaaatgaac atcctcttcc gttaaatatt ctttggttaa
    17101 agcgacaact tcttcgcctg ttaaattcac ttctttcggc atctctactc tccaattctt
    17161 cctaccattt tatcactttt ttaagaatat gaaaactaga ttggaacaga ataagaaaaa
    17221 aataattcaa aattgcttga taattctgaa ttattggccc ataatatact acgaagttag
    17281 attttaaact taggtgatag aagaagagat agaagaacgg aaaccatatt gtaacccaaa
    17341 gactttctga cttccccaat tccattgaag atacgaaaga taaacggtgg aactcgtatc
    17401 acatacactg gtaccttgac tggattttgg aattaatact caatgaaaat ccaagagcaa
    17461 actaggaaac tagccgcaag ctgttcaaag cactgctttg aggttgtaga taagactgac
    17521 aaagtcagtc acatatatac ggcaaggcga cgttgacgcg gtttgaagag atttttgaag
    17581 agtataaaaa tcctcaagat actttcttct atcctttagt ttataaggag aatacctatg
    17641 aaaaaactgc tatttctatc tttgctctcc taatgttagg agtttgctgc ctgttcctat
    17701 tcagccagca aagctataaa aaactcgttc aatactatgc taacgaccag aacctgccca
    17761 gtaggataac ttatagtgaa tatagcgaca aatgagaagc caactacggt agcactctaa
    17821 acatcacgtc tatcaaacaa gctaatgacg gagtttatgc aacctatgaa gggcaattga
    17881 cacctttcca atattgataa attgataacc aacctgtctt catctagtta tgctggtttt
    17941 taagttcatt ttaaatcctt acctattctc cctaactgtg ctatacttaa tttatactca
    18001 atgaaaatca aagagcaaac tagaaagcta gccgcaggct gttcaaagca ctgctttgag
    18061 gttgcagata aagttgacgc ggtttgaaga gattttcgaa gagtattagt acattctttg
    18121 agattggagc tagtatgaaa atccataaaa ccgtgaatcc tgttgcctat gaaaatacct
    18181 attatctaga aggcgaaaag cacctcatcg tcgtcgatcc tggtagtcat tgggaagcca
    18241 ttcgtcagac aatcgagaag atcaacaaac cgatctgtgc tattctcttg acccacgccc
    18301 attatgacca tatcatgagt ctggacttgg ttcgcgagac gtttggcaat cctcctgtct
    18361 atatcgcaga gagcgaagcc agctggctct acactcctgt cgataatctc tccggtctcc
    18421 ctcgccacga tgatatggca gatgtggtca caaaacctgc agaacacacc tttgtctttc
    18481 acgaagaata ccaactagag gaatttcgtt ttaaggttct accgacccca gggcactcta
    18541 tcggtggtgt ttccctagtc tttcctgatg ctcacctagt cttgacggga gatgctctat
    18601 tccgcgaaac tatcggacgg accgaccttc cgactggtag catggagcaa ctccttcata
    18661 gtatccagac ccaactcttc accctaccaa actacgatgt ctatccagga catggtccag
    18721 ctactactat cgctcacgaa aaggccttca atcccttttt ctagcaagat gatgacaatc
    18781 gaaatttaag taaactatcc agcaaatctt tctattacaa aaggcatcct atcaaggttt
    18841 tcacacatga ttggatgcct tttttctgat gactagattt tttgtattac caaataatca
    18901 cgcgctcctc tggtgaacgc cacattccgt ctccttcttt gacatcatag gttgtaaaga
    18961 aatcgtcgaa gtttggtact tgcacattga cacggagttt ggctggtgcg tgcacatcga
    19021 cgctagccaa aagtttcata aattctggtc gacctttcat gcgccagatg cgaccgaagt
    19081 tgtagaagaa ctcttctgct gagaagtctg cttctctctt agctgcttca agcgctgctg
    19141 cgattcctcc caagtcagcc acgttttctg atacagtcaa tttaccgtta atggttgctc
    19201 cataagaatc ctgtccatca aattggtcaa tgactttttg tgttttctcc ttgaaggcag
    19261 catagtcgct ctctgtccac caatccttga ggctaccatt ttcgtcaaag gaagccccgt
    19321 tagtatcaaa ggcgtgggaa atttcatggg caatcactgc cccaatacca ccgtagttag
    19381 cagaagatga ctgatgcaag tcatagaaag gcgcctgtaa aatggccgct ggaaagacaa
    19441 tcaggttctt ctgaggattg tagtaggcat tgaccatatg agcaggcatg ccccattcct
    19501 tatagtctac aggctggttc cacttactcc aactgtgctt gatttccaca cgcgcaaagg
    19561 ctagagcatt ctcaaaaaga ctggcagttt cattcactac cttatccttg taacgtgcag
    19621 gcaattcttc tggatagcca atataaggtt tgatcacatt gagcttcacg atagcctgtt
    19681 tacaggtttc tggagtgagc cagtcattct taagcagacg ctccttatag acatcaatca
    19741 tggttgccac ttttttctcc acatccgcct tggcttctgg agagaacttc tcacgggcgt
    19801 accaaagccc cagggcttgc ttgaaaggtt cttgtgctag atgataagct gctttgacct
    19861 tatcttttgc ctctggaact ccagaaaggg cacggctgta ggcaccagac aaaacacgga
    19921 tatcctctgt taaatagctg gttgaaagat tgacaacact caaaatcaag gttgctttaa
    19981 ggagagacca ggattcctca ctgtagaatt gctctgctgc ttgccagaaa cgttcctcgt
    20041 ctacaataac cttgtctggt aattgcccaa taactgcttt gaagaagtca tccaaaggta
    20101 gggcaggcgc gaatttcttg aaatcttcgt aagaatatgg atgatagagt ttagcatatt
    20161 ctgaactttc ttcattagag agcaccactg ccgcaactcg gcggtccaat tcaagtcttt
    20221 tttctagcaa gtcttcaatt tcttcatcag agaaatcata agccttgagg agatttgcgc
    20281 tgctttcttt ccaaagagtc aagagttctt cgcgctgagg atgttcttct gcatagtagg
    20341 tcgtatctgg caagattgtg cttggagcgc tagcccatag aacattgatt ctagcatcca
    20401 taaagtctgg cgatacacca aaaggaagga agtttggttt tcctgcaagc tcaaactctg
    20461 ctagtttagc tgtaaaatcc gcaaaagttt ccaattcttg gaattcttta aggagtggta
    20521 agacaggtgt gataccgtca gcttctctct tgtcaaaatc acgaactagg cggtggtatt
    20581 tgacaaagtt ttccaagata gcatcctcag gcacttcttc acctgctaac cacttgtctg
    20641 ttgtcgccaa catcaggtct tcaatttcct ggtctaaatc aacaaaacct cctgtttgag
    20701 acttatctgc tgggatttca gctgtctgtt gccattctcc attgatagca tcataaaaat
    20761 catcttgata acgtgtcatc ttgttctcgc tttcatttgt atttgcattt atcttaacaa
    20821 aaatcactag ggaa
//
LOCUS       DAVLWA010000028        20437 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLWA010000028 DAVLWA010000000
VERSION     DAVLWA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.028"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(110..259)
                     /locus_tag="VYN80_001012"
                     /pseudo
     CDS             complement(110..259)
                     /locus_tag="VYN80_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(413..2044)
                     /gene="rlmD"
                     /locus_tag="VYN80_001013"
     CDS             complement(413..2044)
                     /gene="rlmD"
                     /locus_tag="VYN80_001013"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1540942.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLRVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLFRRFDLKPYDEKEHSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2118..3092)
                     /locus_tag="VYN80_001014"
     CDS             complement(2118..3092)
                     /locus_tag="VYN80_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1540943.1"
                     /translation="MRKKLFLTGAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3166..3780)
                     /gene="pmp23"
                     /locus_tag="VYN80_001015"
     CDS             complement(3166..3780)
                     /gene="pmp23"
                     /locus_tag="VYN80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1540944.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3780..4754)
                     /locus_tag="VYN80_001016"
     CDS             complement(3780..4754)
                     /locus_tag="VYN80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1540945.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4762..6018)
                     /locus_tag="VYN80_001017"
     CDS             complement(4762..6018)
                     /locus_tag="VYN80_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1540946.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6080..6511)
                     /locus_tag="VYN80_001018"
     CDS             complement(6080..6511)
                     /locus_tag="VYN80_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1540947.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6513..7115)
                     /locus_tag="VYN80_001019"
     CDS             complement(6513..7115)
                     /locus_tag="VYN80_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1540948.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7099..7938)
                     /gene="prmC"
                     /locus_tag="VYN80_001020"
     CDS             complement(7099..7938)
                     /gene="prmC"
                     /locus_tag="VYN80_001020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1540949.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(7938..9017)
                     /gene="prfA"
                     /locus_tag="VYN80_001021"
     CDS             complement(7938..9017)
                     /gene="prfA"
                     /locus_tag="VYN80_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1540950.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9014..9604)
                     /locus_tag="VYN80_001022"
     CDS             complement(9014..9604)
                     /locus_tag="VYN80_001022"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1540951.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9727..9909
                     /locus_tag="VYN80_001023"
     CDS             9727..9909
                     /locus_tag="VYN80_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1540952.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10045..11418
                     /gene="mnmE"
                     /locus_tag="VYN80_001024"
     CDS             10045..11418
                     /gene="mnmE"
                     /locus_tag="VYN80_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1540953.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11465..12400)
                     /gene="dapA"
                     /locus_tag="VYN80_001025"
     CDS             complement(11465..12400)
                     /gene="dapA"
                     /locus_tag="VYN80_001025"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1540954.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12456..13532)
                     /locus_tag="VYN80_001026"
     CDS             complement(12456..13532)
                     /locus_tag="VYN80_001026"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1540955.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASACSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14118..14190)
                     /locus_tag="VYN80_001027"
     tRNA            complement(14118..14190)
                     /locus_tag="VYN80_001027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14155..14157),aa:Thr,seq:ggt)
     gene            complement(14290..14799)
                     /locus_tag="VYN80_001028"
     CDS             complement(14290..14799)
                     /locus_tag="VYN80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1540956.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14797..14893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14922..15353
                     /locus_tag="VYN80_001029"
     CDS             14922..15353
                     /locus_tag="VYN80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1540957.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15435..15812
                     /gene="mscL"
                     /locus_tag="VYN80_001030"
     CDS             15435..15812
                     /gene="mscL"
                     /locus_tag="VYN80_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1540958.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15854..16948)
                     /gene="hemH"
                     /locus_tag="VYN80_001031"
     CDS             complement(15854..16948)
                     /gene="hemH"
                     /locus_tag="VYN80_001031"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1540959.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17103..18326
                     /gene="pepT"
                     /locus_tag="VYN80_001032"
     CDS             17103..18326
                     /gene="pepT"
                     /locus_tag="VYN80_001032"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1540960.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18362..18991)
                     /locus_tag="VYN80_001033"
     CDS             complement(18362..18991)
                     /locus_tag="VYN80_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540961.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            18956..19177
                     /locus_tag="VYN80_001034"
     CDS             18956..19177
                     /locus_tag="VYN80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540962.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19254..19795)
                     /locus_tag="VYN80_001035"
                     /pseudo
     CDS             complement(19254..19795)
                     /locus_tag="VYN80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19899..20437)
                     /locus_tag="VYN80_001036"
                     /pseudo
     CDS             complement(19899..20437)
                     /locus_tag="VYN80_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5893 a         4313 c         3860 g         6371 t
ORIGIN      
        1 aagcagcaca ccaactattt attttttgag ttttaaactt tggtagaagt cttgaactcc
       61 ttatatttac ataactattt gcctctataa ctgttatgac acgaaaccaa caagcaagtt
      121 caagcacctc catatcttta ttcaaatgag gactaagata ttcacaaact ttatcataaa
      181 cctctttatc ttttttcata aaaggggcat acaacttagc aaatttatcc taaaattttt
      241 tgttgccttt atcaatcatt tcaacgctcc tttaaatata aaaatgatcc tatcaatctt
      301 tatattgagc ctattttcta attgatacct tgcaagaaag gaataatagc tgttcctata
      361 ccaagtagga atgtaataaa tgcagaaagt catatactgt cattcttttc tattaaatca
      421 ttttgaagtg tctcaaagca tccatgatgg cttcttcttt ttcaggtgta cactgtggaa
      481 taatttgttt atccttttta gacttgttgt aatgttctcg taattctatt ccacatttct
      541 tttttatctg tgcaatatat aatgtcgaaa cttttaattc aaatttattc caaacatatt
      601 ctttgatttg agcatatgtt gctttgctct ccgcacttgt caaatccatc tcatccagct
      661 caatttcaac acttatgtgc ttatcgacat cgagtttgga caaaagtgct accgtctcga
      721 cgtgatgcgt ttgaggaaat agatccaccg gctggacttt cttcaattca tatcccaact
      781 cttggtatag tttaatatca cgcgccatgg ttgcgacatt gcaggagata taggcgatgc
      841 gatcggctcc tgtttgggcg cttgctttga taaagctttc tgtcaagccc ttgcgtggag
      901 gatcaaccaa gataacggtt ggttgaatcc catctttaag ccaattcttc atagcatttt
      961 cagctgtgtc acagacatag tgggcgtttg aaatattgtt cagctgagca ttttttttac
     1021 tattctcaac cgcttctgga atcagttcaa caccgtagac ttctttgaca tgcttggcga
     1081 ctgataaacc aatggttcca ataccagaat aagcatcaat aaccacgtca tcttttttta
     1141 actctgcaaa gtcaatggct gtttgataga gtttctccgc catttcagta ttgacttggt
     1201 aaaaggctgg gccagcgatt tggaagtcat ttcccaacat ctggtccgta atatagtctt
     1261 gaccataaag agtgcgccac tccttaccaa aaatcgcatt ggtattctgg tcgttgatat
     1321 tttgcatgac agacacaatc tctgggaact gcttgataac ttgttcaatc aattggtcaa
     1381 cacgaaaaac ttttggacga gttgtcacca aaacgaccat gatttgccct gaatagtgac
     1441 cacgacgcac cacaagattc cgaatcaatc cagaatgttc cttttcgtca taaggtttta
     1501 aatcaaaacg acggaacagg tctcgaagag ctactacgac ttggtcaatg acaggatcct
     1561 ggataaagaa atcttcaagg ggcatgaggt tgtgcgaatt cttacggaaa aatcctgttt
     1621 ccaagacacc attcactcga cgaacgggca cctgcgcctt attgcgatac ttgactggat
     1681 gttccatacc aagcgtttca gcaacttcta catctgcaat tccagcaatc ttgtagagac
     1741 tgtccttgac ttgcttggtt ttaaacttga gctgttctgg ataagaaagg tgtcctaaat
     1801 ccgcgattcc tgaacgcagg taagccaaat ctagatcttg attacggtgt ggtgactgga
     1861 caaggtattt ttcaactttt ccaaagccaa tctttttatt gaccctgagg acacgcatga
     1921 gaattttttc actcggtaaa gcattctcta caaaaaagac caaaccatct accttggcaa
     1981 ctcctgcccc ttcatgggtc aaatcaacaa tttcaacttc tacaatatca tttttcttta
     2041 acattctttt ctctttctat tggccgacaa aaaagacggc aacgttacgg ttaccatcca
     2101 ttataccatg aaatttctta agaaccaaaa ctcttgaaga agttgacaag ggcttgccag
     2161 agggagctta agaagttacc accgtcctct agcgccttat ccgcatcaaa gttaaggttg
     2221 atatttttaa aactgtcgcc agcttgtgat acgatgcttt gtttaaggtc atttagggtt
     2281 ttagtgaaat ctgcattgct gaggatatca ctctttgaga gattcaaggc aaaattgatg
     2341 atgatattga tctggtttcc tgttatgacc tgatcaagtt tgtaattttt taaggtatct
     2401 tcaacaatct tgcgtacatc ttcttctgtc agatttccct tgctttcttt agctttggcg
     2461 agtcctgact tgatatcagc tagggcaacg tttaatttat tagcatcata gcctgatttg
     2521 tccttgtttt cagcattgat atctgacaaa gcctttagct cttcttgagc caaatcttta
     2581 ttggcttgcg gcaccttggc tccattagct tctagcgaat agtagatccc agccaaagca
     2641 ctctcacctg taactggaat aggggctgct acagtgattt tggcatgttc catacccagc
     2701 gttactgctg cgtttcggta catatcctga gtcaccttag tgatattttc tggtgtttca
     2761 atcttgacct caagtggcga tttgtcacct agcttttgaa tcttggctga tgaatacaac
     2821 tgtaagctag agtcattggc cacattcatg attttagaat aaacatcagg tgtcatggtc
     2881 ttgagttctt tggtatctgt tgaggcattg tagcccagtt ttttaagagt ttgatttttt
     2941 tggtcttcag atagggagga acctaggaca tattcaggtt ggacataggt ttcatcgata
     3001 actttttgaa catctgttgc tgcatgggcg ctattcatag ctgttactgc ccacaagacc
     3061 gcagcaccgg tcagaaagag tttctttctc atagggaatt tcctccttta cttttctaga
     3121 gtaatatatc tatcttaaag aaaacttata acaaaaacac ctggtctagc cagatgttga
     3181 aaagagagtg aaacatttga tgatgtaaag gttaagtcgt acctgtctag aataataata
     3241 gtttcctcca tttacataga gttcagcacc gtgaaaaatg gaaatggggt gaatataact
     3301 ataagtcttt ccagtcctat taccaagcaa gggggcaaca gtctcacgag agtactgttt
     3361 ggctagagcc aaggtatttt ccttgccatt ttgggcgata aaatcgatat aggcaggtcc
     3421 aaaattatag gcttgaacag ctgtccagat atctacaccc ttcttctgcg ccagatagag
     3481 attgcctgtc agagtttgaa tgccttgccg aatgctagag gcattatcat tgatggtgtt
     3541 ggtggaacca cttgcagact cactagactg cataacatcg ccttcttttc cttttgtttc
     3601 agtataaatc atagcaagca caagctcttc gtttgctggg gtgtcttttt cactcaatat
     3661 ttctcgcacc atgggttgat aggtcatgac ttgtttgaca tcttgatgaa cgcggtaagc
     3721 tttatagcca gcaaaaagga agactgctag tacaagcact cttcgaattc gtttaaacat
     3781 tatttacttt ggatatcctc gatatttttg attaagatag agtaggttcc attttcgttt
     3841 tggataaact caacagactc ggcgtcttga tagacgttat tgggaacgat gagctcaatt
     3901 ccatttgata aggagagttt ttggttttca aatttcttta attggcgact ggcatcaatt
     3961 tcatcaaatt gaacaggttc tggtacggct tctttgactt ggtcaataaa gctcaaacga
     4021 gccgtcagat tgttgtcaaa aaggtcatta gccaatttct caggtgacaa ttcattgctt
     4081 tcttctaggt tgttgaaaat agctgatttg accttggatt gaaattgaaa atcatctgtg
     4141 ttaaaagatt cagcaattct ctgggctgtt ttttccagtt ccttgataga ttttttagga
     4201 gaaatcttag gagcgacagc aagaagatta tctgaaaaat agttcaaaaa agtcccgttg
     4261 tacttgattc gtttttcaat caggtgatac ttgcgactct gaagattgac caccaaggcc
     4321 tcgtcagcac ccgttccaaa tccaggcagg ttattctgag tcagcttgat tggattatca
     4381 acttctcctc cgaggtgggt caaggtctcc cgcagggcaa ttcgcaagaa agcgaaatgt
     4441 tctacacctt ctttagaaaa ttgaacaaaa atcaagtcat tggtcttgag attttcagaa
     4501 atgctaaact cctctttcca gagattagcc agcgttactg atgtctccaa caaatcgtct
     4561 gtaatatgat tgaagaaggg attttcttct tcgaaaatcc cagtcttggc ttcatctgaa
     4621 tacacatgtt caattttttt acgtaggtat tcttcgattt ttggagtaat attgagaaac
     4681 ttatctgcta agaacagctc ggtatcatcc ggactgaact ggtgaataat ggctttctta
     4741 atataaatat ccataaaagt tttagtcctc gtataatggg aaggcatctg tcaattcttt
     4801 gactgcactt ctcacttctt ctaatacagc ctcattttct gaattcttaa gggttttaat
     4861 gatgagttca gccactttgc gactttcttc ttcaccaaat ccacgtgcag taatagctgc
     4921 tgctccgata cgaatcccac ttgtcttgaa tggcgacaag gtttcgtaag ggattgaatt
     4981 tttatttagg gtaatattga cttcatccag caagttttga gcaacttttc cgttttctac
     5041 aaccttagtc acatcaacaa ggaagagatg gttttcagtt ccacctgaaa taatacggaa
     5101 atcagggtct tgcaagaaga catctgccat agccttgctg ttcttaatta cattggcagc
     5161 atattccttg aaggctggat ccaaaacttc tttgaaggaa actgccttag ccgccacaac
     5221 atgctctaaa ggaccgccct gaatacctgg gaaaatagct gaattgattt ttttagcaag
     5281 ttcttcgtca ttggtcaaaa tcaaaccacc acgaggtcca cgaagggttt tgtgggtcgt
     5341 tgttgttgtg atatgagcgt atggaactgg gcttggatga aggccagccg caaccaaacc
     5401 agcgatatgg gccatgtcca ccatgagctt cgcaccgaca gcatctgcga tttcacggaa
     5461 ttttgaaaaa tcgataattt gagaatatgc tgaagcacca gctacaatca gttttggttt
     5521 tacttcttgg gcttgtttca agatagcatc aaagtctaag agttccgttt caggatcaac
     5581 actataagaa acaaagttgt aggtttgacc agagaagcta acaggagccc catgagtcaa
     5641 atgaccacct gatgccaaat ccattcccat aaccgtatca cctggctcaa tcaaggacat
     5701 gtaagccgca cagttagctt ggcttcctga atgtggttgg acattggcaa atttagcacc
     5761 gaaaatttct tttgcgcgtt caatagcaag agtctctaca acgtctacta catcagttcc
     5821 accataataa cggcgtcctg ggtaaccctc ggcatattta tttgtcaaga tagacccttg
     5881 agctgccata acagccttgg aaactacgtt ttccgaagca attaactcga tattattttg
     5941 ttggcgttct tcttctttgg caatagcatt ccagagatca gcatcatatg ctttaaaatc
     6001 atctttgtca aaaatcatag gtcttctcct ttattgtgtg actagtccat tagtttgatt
     6061 ttacaataag aaaatcaaac taaaagatgc gaataaaccg tttctgcatt ttatcacaag
     6121 tatagccaac tttttcataa aatgcatgag cacccagacg atgattggca gaatttaagc
     6181 ggataaaccc ataaccacat ctttttgctt cttcttccaa cccttgtagt aaacttttac
     6241 caataccttg accttgcgct tgaggtgaaa ctgctaaagc taagatatta aatcctgctt
     6301 tggaatagag tgattcgtaa acttcagcgt ggacatatcc aagtaagaca tgattagctg
     6361 catcctcata gccaagtagg aaatgatggg aatcctgaga cagtctagct agttggctag
     6421 ccgtttcctc tggactaaaa gtataaccca aagcctcttg gttgatgtca catatcgctt
     6481 tcacatctgt ttcttgcaaa tctcttagca tttcaagcct cctcaaaaga aatctctggc
     6541 aaccgagcaa gaatatcttc tcgtttaatt gcgccttggc gtaagatttt caccttgtct
     6601 ccagacaaat ccacaatagt tgaatcctgt ccagttagaa aagcatcgtc ttccagaccc
     6661 agaacctctt ggtcaaaatc cttcagaatt tgttcaaagg ttacaccact tgcctgacct
     6721 gagatattgg cagacggccc aatcaaggga cctgtctctc gaattaaatc cagtgtgata
     6781 gggtgactgg gcatccgaaa tccaatagtt gcaaggtcag aatttaccca atagggaact
     6841 cggtcattgg cttcgagaat aatggtcaag ggacctggca aaaaggtctc tacaagtttt
     6901 tgtagataag ttggctgatt cttagaaaag tgcaagatat cctcgaaaga ggcgatattg
     6961 agattgagtg ccttatctct aggacgacgt ttgagttggt aaacatggtc aactgctttt
     7021 tcatctaagg ccttggcaaa aagaccataa acagtctctg taggtagaac gacagctcca
     7081 ccattttcca actcttgtct aatcctgtcc atcatcaacc acaaccatcc tattttgacc
     7141 aaattggtct ttgagtgttc gaactttttt ttcaggaaga tgttttctaa aaagttcggg
     7201 aacactttga ccttgcttgt atccaatttc aaggtaaatc ttaccaccat ccttgagata
     7261 gtcttttgca tcttccgcaa ttctacagta aatagctagg ccatcctcgt ctgcaaagag
     7321 agctagatga ggctccgaat gcaaaacatt caaacctacc tctgactcat cttcacgaga
     7381 gatatagggt ggattggata caattatatc atatttttca gaaatttctg caaaacaatc
     7441 agatttttta aaaaatatat taagattttg atcgctagca ttctctgatg caagctctaa
     7501 ggcctcttga gaaacatctg ctgctgtcac tgaccaatct ggtctatttt ttgctagagc
     7561 gagagcaata gctccactac ctgttccaat atccagaact gagagattcg tctcaagatt
     7621 ttcagttaga ataagctcca ctaactcctc tgtttctgga cgaggaatca aaacccgttc
     7681 atccaccttt aaatgcattc cataaaaatc tgcctgtcca atgatgtact gagctggctt
     7741 gtgagctgct agttgctggt aaatatcttc tacaaattgt ttttcttcct ctgttgtcac
     7801 ctcctgctgg agggcaaaaa taaagtctgt aaaagataga tttttcagac tacgatagac
     7861 aaaagagagg ctttccgctt cctctccttg tcttatcaac tcttcttcaa aatttgaaaa
     7921 taattgagct aatttcatta tttgtttaat tcttctagtt tttgtgtttg gtcataaagc
     7981 accaaggcat ccacaacttc gtccaattta ccagacaaaa tcgtatctag tttttggagg
     8041 gtcaagccga tacggtggtc tgtgacacgg ttttgtggga agttataagt tcggatccgt
     8101 tctgaacggt caccagtacc gattgtcgac ttacgctcag cgtcttgttc atcctgagca
     8161 atctgagcaa agtggtcagc gacgcgtgca cggataatct tcatagcctt ctcgcggttc
     8221 ttctgctggg tacgttcttc ctgcatctca accttgatat tggttggcaa gtgaacgata
     8281 cgaacggcag tcgcaacctt attgacgttc tgtccaccag caccagaggc gtgatagatg
     8341 tcgacacgaa ggtcttttgg atcaatgtcg tattcaacct cttcaacttc tggcataaca
     8401 agaactgtcg ctgtcgaagt atgaacacgg ccttggcttt ctgtcacagg aacacgttgc
     8461 acacggtggg cacctgattc atacttaagc ttagagtata cagactgacc tgaaaccata
     8521 gcaaccactt ctttaaaacc accgacacca ttcatagagg cttccatgac ttcaaagcgc
     8581 caaccttggg cttccgcata cttttggtac atagttagca aatctccagc gaaaagtgcc
     8641 gcttcgtctc caccagctgc tccacggatt tcaaggatga tattcttgtc atcgtttgga
     8701 tcctttggaa ggagcaaaat tttcagtttt tcttcatatt cttctttttc agccttggca
     8761 tctttgagtt cttgcttggc catttcttcc aagtccgcat ctccgcctga ttccttaatc
     8821 atctcttcgg catcgacgat attttgaagg acttgtttat actcacggta ggctattacg
     8881 gtgtcacgat tggaagcttc ttcttttgaa agctccataa aacgcttggt gtctgaaacg
     8941 acatcagggt cactcagcaa ttctcctagt tcttcataac ggtcttctac agcttgtagt
     9001 tgatcataga tgttcatttt tcttcattct ccttattgat ttcaggggca aaataatgtt
     9061 tacggcaaac cgagatatag gtttcattac caccaatctg gatctgttct ccatcgtaaa
     9121 cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa tactgacaga
     9181 tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag atatttggaa ccttcgaaca
     9241 attcattgcg aaagtcattt ttcaagccaa aagccatgac gggtatgtct aactcgtcca
     9301 caacacgagc taggtcataa acatggtggc gtttgagaaa ctgggcttca tcgaccaaaa
     9361 cacagtaagg tttttctggt aggtctcgga tatagccaaa gatatccgtt gtttcctcaa
     9421 tcgcaagggc agggcgtttc atgccaattc gactcgacac atagccaaca ccgtcacgcg
     9481 tatccagagc cgaggtcata atcacaacac cttttccttg ctcctcgtag ttataggcca
     9541 ctttgagaat ctcaatcgtt ttaccagagt tcatggtccc ataacgatag tacaactgtg
     9601 ccatgtttct tgcttcacgt ccatttctaa atttttgcta cattctagta tatcataatt
     9661 ttcttaagct ttaaacggca aaatgtggta aaatggaaga aatcaaaact agtggaggaa
     9721 gctattatgc catttgtacg catcgattta tttgaaggac gcacgctcga gcaaaagaaa
     9781 gctcttgcta aggaagtaac ggaagcggtt gttcgcaaca ctggagcacc tcaatctgct
     9841 gttcatgtca tcatcaacga catgccagaa ggaacttatt tcccacaagg ggaaatgcgc
     9901 accaaataag ctagcttaag cagaattgct taggcttttt caatctccaa gtagcaccca
     9961 ttgaagaaat agtctaaatt tgttacaatt tgaaaagaga cttggaaaat tccaagaaaa
    10021 gagcaattaa ttaaaggaaa cattatgatt acacgtgaat ttgataccat cgctgctatc
    10081 tctactccac taggtgaagg ggctattggt attgtccgcc tgagcggaac agacagtttt
    10141 gctattgtgc aaaagatttt taaaggaaaa gacttgaaca aggttgccag ccacactctc
    10201 aactacggtc acattattga tcctctgact ggtaaagtca tggacgaggt tatggttggg
    10261 gctatgaagt ctccaaagac cttcactcgt gaggatatta tcgagattaa cacccacggt
    10321 gggattgcgg tgaccaatga aattctccag ctagctattc gtgaaggggc tcggttggca
    10381 gaacctggtg aatttaccaa acgtgctttt ttaaacggtc gcgtagactt gacacaggca
    10441 gaggctgtga tggatatcat ccgtgccaag actgacaagg ccatgaacat tgcggtcaaa
    10501 caattagacg gctccctttc tgacctcatt aacaataccc gtcaagaaat cctcaataca
    10561 cttgcccaag ttgaggtcaa tatcgactat cctgaatatg atgatgttga ggaagctact
    10621 actgccgttg tccgtgagaa gactatggag tttgagcaat tgctaaccaa tcttcttaga
    10681 acagcccgtc gcggcaaaat ccttcgtgaa ggaatttcaa cagctatcat cggacgtccc
    10741 aacgttggga aatccagcct tctcaacaac ctcttgcgtg aggacaaggc tatcgtaaca
    10801 gatatcgctg ggacaacacg agatgtcatc gaagagtacg tcaacatcaa tggtgtacct
    10861 ctcaaattga ttgatacagc cggtattcgt gaaacagatg acatagtgga acaaatcggt
    10921 gttgagcgtt cgaaaaaagc cctcaaggaa gccgacttgg ttctactagt gctaaatgcc
    10981 agtgaacaac tgactgcgca agacagacaa cttcttgaaa ttagccaaga taccaatcgc
    11041 attattctac ttaataaaac cgacctgcca gaaacgattg aaacttcgaa actacctgaa
    11101 gacgttatcc gtatttcagt ccttaaaaac caaaacatcg acaagattga agagcgaatc
    11161 aacaacctct tctttgaaaa tgctggcttg gtcgagcaag atgctactta cttgtcaaac
    11221 gcccgtcaca tttccctgat tgaaaaagca gttgaaagcc tacaagccgt taatcaaggt
    11281 cttgagctgg ggatgccagt tgatttgctt caagttgact tgactcgtac ttgggaaatc
    11341 ctcggagaaa tcactgggga tgctgctcca gatgaactca tcacccaact ctttagccaa
    11401 ttctgtttag gaaaataaga aaaatccatg atccttcatt cggtcatgga ttttattgtc
    11461 tttattagta atctggtctt aagacccctg ttacagttgc cttagttgct tcgtagtcgc
    11521 catctacgac aaccttgata atgcgtttgg catcttcttc tggtgctgga acaagaggta
    11581 gacgagtggg tccagcttca aatcccatat agttaagaat tgccttaact ggagcaggac
    11641 ttggataaga gaagagagca ttaaccttag gaatgaattt acgctgaatt gctgcggctt
    11701 tcttcatatc gctttctgca atggcagtaa acatctcgtg catttcatcc ccatttgtat
    11761 gagaggcaac agaaataacc ccatccgccc caaggttcat ggcatggaaa gcatctccat
    11821 cctcacctgt ataaatcaag aactcttcag gcttgtgctc aatcaagtaa gccatattag
    11881 ccaagctagt acattctttg acaccgataa tatttggatg gtcagccaag cgaagcatgg
    11941 tttctggagt caattcgaca actacacgcc ctggaatgtt atagataata attggtaggt
    12001 cagaagcatc tgcaatagcc ttaaagtgct gatacatccc ttcttgagaa ggtttgttgt
    12061 agtaaggaac aatagcaagc ccagctgcga aaccaccaaa ttccgctact tctttgacaa
    12121 actcaataga gtcacgcgta tcattagtac ctacacccgc aatcaaagga acgcgtccat
    12181 tgacaacctt ttgtacagcc gcaaacaact ccaactcctc atcgtgggtc aaagttggac
    12241 tctcagcagt cgttcctgcg agaagaattc catccgtatg atgggccaat aaatgctcaa
    12301 tcaaggctgg aatagcatca aagttaatgg aaccatcctc atggaagggg gtaataaagg
    12361 ctgtaatgat tttacattct tttaaatctt gataagacac gaaaacacct ctctatttca
    12421 aagaaggagt tcatctgaac tcctaaacga tatgactatt ttaattcaaa tttcaattca
    12481 gctgttggac gaaccaatcc acgttcatga agagtttcag caatctgaac tgagttccaa
    12541 gcagcacctt tgagaaggtt atctgaaaca acccacatgt gaattccttt ttctgcatcc
    12601 aagtctttac ggatacgacc aacaaaggta tcacgtgaac caactgcatt gatggcttga
    12661 ggatagattt gatgaactac atcatcttca agaacagcac ctgggaagga tgcgatagct
    12721 gctttgactt cttcaattgg agccacttcc tttgtttcaa tataaactga ctcagagtga
    12781 gctgacaaga ctggaatacg cacacatgtc gcagatactg caatgctatc atcttccata
    12841 attttcttag tttccttggt catcttcatc tcttcgtacg tgtaatcatt atcagtgaag
    12901 acatcaattt gtggaagagc gttaaaggcg ataggataat gtttcttgtc accacctgaa
    12961 ggcaagattt ccgcatgcaa atcacgtggt ttcactccat cattcaagac ctcacgaagt
    13021 tcacgttgtg tctcaagaat tgctcccata ccagcacctg aaacggcttg ataagttgaa
    13081 acaatgatac ggtccaagcc ccatttttgg cgaaccggct caagagccac catcatttgg
    13141 attgttgaac agttagggca ggcaatgatt ccgttgtggg catcaagtgc atgagcattg
    13201 acctctggaa caaccaaagg aacatctgga ttttgacgga aataagatgt attatccact
    13261 actaccacgc cagcttttac tgcgtatggt gcatacttag ctgatgtcga accacctgct
    13321 gaaaagagag caatatcaac tccttcaaaa gctgtttcag tcgtttcttc aatcgtaata
    13381 tcttgatctt taaatttcaa tgacttacct gctgaacatg cagaagcaag gtaacggatt
    13441 ttatcgattg gaagtgttga ttcttccaac atttttatca tctgagcacc gacagcacct
    13501 gtcgcgccga ctacagcaac tgtatatccc ataaataacc tctttcggaa ttttctaaaa
    13561 atttctataa tagaactatt atactacttt ttccatgaat tgcaaagctt tttcatcatt
    13621 ttttggaaaa taaaaatccc caatcgctag actagggatt aagaggcatc ttcatgtgat
    13681 gagcaggttc acacaactca tcaaggtccg ctcctgcgtt atgacctcct tatgctcaat
    13741 agtagcccga agactaccta tcgactcatc gcatctacta ttctactaaa gagaatgact
    13801 tttgtcaaca gacgaaactc tttatactct tcgaaaatct cttcaaactt cgtcagcgtc
    13861 gccttgccgt atgtatggtt actgacttcg tcagtttcat ctacaacctc aaaaacatgt
    13921 tttaagctga cttcgtcagt tctatctaca acctcaaagc agtgctttga gcaacctgcg
    13981 gctagtttcc tagtttactc tttgattttc attgagtatt agttttattt acacaggtgc
    14041 ataagaaata tattggaaaa attgggggca gacataacaa aaacccttgt aaatcaaggg
    14101 cttttctgtc tatttaatgc taattgccgg gattgaaccg gcgacctcat ccttaccatg
    14161 gatgcgctct gccaactgag ctaaatcagc ttacttaaga agtatactat attcagaaaa
    14221 acttgtcaag tttccttaga aattttccat aggaaaaagc caggtaagaa ctacctagct
    14281 tatcttcttc tatttactta aatcaattcg acgaccaatt ttaccaataa taatcgctcc
    14341 aataatcaat gaagcaaatt caccgattcc tgttgtaaac caagtaagga agaatggtaa
    14401 ttctgctaca atatttaact ctgcagcgat agtaaacatg gaaattgaaa agaggattga
    14461 aaagaagaaa tgatcttttc gaatcaatcc gttaaagagg taatccttgc tatattttgc
    14521 aaaaagccaa acacctaaac tgaggaatac taaggttgaa ccaccaccaa caaagacatc
    14581 tagcagtccg aagctaaaga aattagcaat catacaaccg atggtaactc cgatgatgta
    14641 cttaggattg taaaaagcca taaagttcat catttctgaa atacgaaact gataagcacc
    14701 atagctgatg gcatttaggg gcggtgtgac agtcaaaacg acgtagatag cagcgacgat
    14761 tgcaatatct gcaacatcac gaatagttaa ttttttcatc ttttctcctt tggcggttat
    14821 ccgcgtgtaa tatgcttggt gaaagaagct aagcaccaag ggttgattca ttcaacctta
    14881 ctagtatagc acaatagcct gctttatgct atacttaaag catgaaaaaa cctattcaaa
    14941 aattttttaa caatgaaatc ttagcctatc tcttttttgg ccttgcgaca actctagttt
    15001 cgattctctc acgactagtc atttatcaac taagtcatca ggaactcctt gctacggcct
    15061 tggcaaatat tatcggtatc ttatttgcct ttatcacaaa tgatacgatt gtatttaagc
    15121 aaaaaaagaa aaatagactt atccgtttag tgaaattttc tcttgctcgc ctttctactt
    15181 ttctgttaga cttgttttta acttttctct ttgtaaccca atttcctcac atcatagggc
    15241 aattcgtaac tgaaaacatt gataagataa atgctattga aacaataatt gcacaaatat
    15301 tcattattat catcaattat atcttgagca aggtctatat ttttagaaaa taatgctcca
    15361 tttatgagcc ttttaattgc cttctataat gatttaggat acaatgaaac acgaaacatt
    15421 gaaaggaaaa cagtatgtta aaaaatctaa aatcgttctt gcttcgagga aatgttattg
    15481 accttgctgt cggtgttgtc attgcctctg cttttggtgc tatcgttact tcacttgtaa
    15541 acgacattat cactcctctt attttaaatc ccgctttgaa agctgctaaa gtcgaacgta
    15601 tcgctcaact ttcttggcat ggagtcggct atggtaactt tttaagtgct attatcaatt
    15661 ttatctttgt gggtaccgcc ctcttcttta ttatcaaggg cattgaaaaa gcacagaaac
    15721 tgactggcat aaagaaagaa aaaactgccg aaaaaaaacc aaccgaattg gaagtccttc
    15781 aagaaataaa agctctcctt gagaaaaaat aatcgattaa aaggatctag cgcaaagcta
    15841 aatccttttt tctttactct ttgggtaact tgcctgcttt ttcaagcatt tttttgataa
    15901 gataaggcat cttaacattc tcccgccctt ttttctcaat cagttttttc acaaattctg
    15961 gcatttgtaa atcttcagat tcagctaaaa tgtttttcgt ctcgtctgaa ggaactaact
    16021 catcaaaggt ctcttcttct gggagaaatt ccttaaactc ttgattttca ttgactctaa
    16081 ctgtattgac caaagcatca atcaaacgag aatctgtatt tggcattggt ggacgatggt
    16141 agtttacccc aaattcctgg cataagtcat aacattctac atcattgtca aacaagacct
    16201 caatatgctc actgatgaaa ctaataggta caaaaatata atggtctgga tgttctgtct
    16261 gttctctgag atactccaaa acatctggtt taatccatgg aataccaata tcactctcac
    16321 tctgccaagt gttggtatat tgttctgaac tcaagcccag tttttcagcg actagcttgc
    16381 tattttcgaa aatttggtct atataaggat caccaaagtc caaggcaaaa atgggcacac
    16441 tatgggctga aaagatgact ttaaagctgt cttgctttac ttcttctttt aaaatcttag
    16501 caatttcatc tgcccaatag tttaagagcg cttcttcctg ataccagtcc ttaatgacca
    16561 aaaattgaat ttgtttgctt tctaaaaatt tttcatagcc catgacagag tagaaagaat
    16621 aatggggctc caaaatcaag caaatacact gttcaattcc gtctgcttcc atttgaccga
    16681 tcacatctgg aataaaggga ctggaaaact tattagcaaa gtagacactg tattcattcc
    16741 ccagcctagc ttctaccaag gcaacctctt gacgggtaat tttttgcaga ggcgtgcctc
    16801 caattcgtac ataattatca tagagagtct gaatctcgtg gtcttggggt ctcaccccac
    16861 gacgaatatt tgtgaaaaaa tcagctacac cttcaaaggt aatctcttct ggtgaaccga
    16921 aggtcatcat taaaattgct tttttcatga ctgcttcctt gtgatgtttt tatcaagttt
    16981 attttatcat taattaatcg ataagtaaac gctaatatag ggacattctc aatcttctgt
    17041 cttttgtttt tctcttcttt ctatgataca atggaaaaaa taaattcaaa aggagttttt
    17101 ttatgactta tccaaatctc ttggaccgct tcttaaccta cgttaaggtc aacacgcgct
    17161 ctgatgaaca ctctactact actccaagta cacagagtca ggttgacttc gcaacaaatg
    17221 tcctaattcc tgaaatgaaa cgtgttggac tgcaaaatgt ttactatcta ccgaatggtt
    17281 ttgctattgg aaccttgcca gccaacgatc cgtctttaac acgtaagatt ggttttatat
    17341 cgcacatgga tactgctgat tttaatgctg aaggagtcaa tccacaggta attgaaaact
    17401 acgatggtgg tgtgattgaa ctagggaatt ctggtttcaa actcgatcca gctgacttca
    17461 agagtcttga aaaatatcca ggacaaacgc tcatcacaac agatggaaca agcttgctag
    17521 gtgctgatga caagtcagga attgctgaaa ttatgacagc cattgaatat ctaactgctc
    17581 atcctgaaat taagcactgt gagattcgtg ttggttttgg tccagatgaa gaaatcggtg
    17641 ttggtgccaa taaatttgat gcagaagatt ttgatgtgga ttttgcctac actgttgatg
    17701 gtggtccact aggtgaactt cagtacgaga ctttctcagc cgctggtgct gaattgcatt
    17761 tccaaggccg taatgtccac cctggtactg ccaaagagca gatggtcaat gcccttcagc
    17821 tagcaattga ttttcataat caacttccag aaaatgaccg acctgagtta actgaaggtt
    17881 accaaggttt ttaccatcta atggatgtga caggtagtgt tgaggaggtg cgtgcaagct
    17941 acatcattcg tgattttgaa aaagatgcct ttgaagcgcg taaagcatcc atgcaatcta
    18001 tcgctgataa gatgaatgca gaacttggga gctaccgtgt cactctcaac ttgacagacc
    18061 agtactacaa tatgaaagaa gtcattgaaa aagatatgac tccaattacc attgctaaag
    18121 ccgttatgga agatctaggt atcacgccta ttatcgaacc aatccggggt ggaacagacg
    18181 gctctaagat ttcctttatg ggaatcccaa ctccgaatat ctttgcaggt ggcgaaaata
    18241 tgcacggacg ttttgaatac gttagccttc agactatgga acgtgcagtt gataccatca
    18301 ttggcatcgt agcttataaa ggctaaaaag acgaggtagc tcagctactt cgcctttctt
    18361 tttattctac tggtttttct tgatttccag tagttgtaga agattctgtt gtttcatttt
    18421 ctgaagttga ttcagcaggt ttagaatctc ttgtattgct tggtttgttt tcgtcgctag
    18481 caatttcaat gttagattct gcagttgcgt ttggttggtt ctcagcactg gtgttatcac
    18541 tatttgcttc agcatttctt gctggacttg tttcttcact tgcgctagct tttgactgga
    18601 tttgatgatt caaaactaga atagcttttg tcgattcaag taaagctgtt ttgtctttac
    18661 tcttagcaga aagttgatct aataatgcat ccaccttatc aaagtccgca tcagatccat
    18721 tattactttc taaataagag tgaagcgaca tgagaatatc gtagagtttt tgatagagta
    18781 caagtgtctg agaatcttgc tcagcatttt ccttttcttg ttgaagggcg ctagcgatac
    18841 gagtcaagac atcttttacc tgactgttta cttcatccaa gtctgcatca gccttgtttg
    18901 tggcagcttt tagattttct acttcttctg ccaaggattg tctgattcct tcttcatgga
    18961 tttgttccaa gagttgattt accttgctca aaagactttc tacttcttcc ttgctatctg
    19021 tcgcagatta ttggttgcca tctaccatgt actcctaaaa caggagagtt ataatccaag
    19081 attacaaggc cttacagaaa taagaaatcc agataagaca atgttcgtcc aagacgctat
    19141 tcgcttcgca cagcagcacg gattcaatat gctttaattt taaagtttag gtgtcaagac
    19201 ctctttttag tgtgcccaaa atttagagaa gtaatcaatc aactaacttt tatttttttc
    19261 aaactttcag taaactgacc taaagctaac tcaatctgtc tttgtagatg cttctgctat
    19321 cagctagaag ttgatctact tttgccaaga ctgccttctc atcaaaagtt ccaggttgat
    19381 agttggattg cagggatgga atcttgtttt tcaaagccgc ttcatatccc ttagtttgaa
    19441 ccttgatgta gtgattgtgg tcgccatgag gaatcacaaa accttctgaa tcttcactta
    19501 taattcgatt ggcatcaaaa ccatgaccat cttcttcctc atgatggaca tgtagtgacg
    19561 gattacttaa tacagaacta gaagaacttc ctacctcttc cgtgttagag tgtgatgggg
    19621 gattgttaag agatgactta ggaatatagt gatagtgatc cccatgtctt actatataag
    19681 catcacctgt atctctgaca atatcattag ggttaaagac atatgtggct gctaattcac
    19741 ctgccgacaa gtcactctca ggaatgaaat gatagtgacc accatgtggt actatcgtag
    19801 attgaaatag aatatgagca aattgataag gggattttaa agtaatttct aacaatgatt
    19861 tagaaactat gatgtgctat tctaaattca actcactata tataaccatc atcggtagta
    19921 taacgtccct gtaattttgc tacagatact tctgcactag ctcctttatc gtctttacca
    19981 tgttcttgtt tttggcgatt gatttcatct tttattcgta cattttctgc atgagcttga
    20041 tctttaaggt aaacataata ctttccatct accttaataa tatatcctcc cttaacctaa
    20101 ctgacgatat cttgatcttt cggctgatag ttgggggctt tcattagtag ctcttcacta
    20161 aagagcgcat ccaaaggaac tttaccatta tagcagtgat aatgatcgcc atgagaagtt
    20221 acataacctt gatctgtaat cttaataaca atttgttttg cttgaattcc ttctttttga
    20281 ctaacctagt ctggagtcaa attttcagtc ttcttagtgt ctttattact gtttacatat
    20341 gaaacacgat ttttatctgt attggcctgt tagctatgtt ggttcagagc ataaacacac
    20401 agacttaagg aaaggataac aacagatcca gctgcta
//
LOCUS       DAVLWA010000029        19257 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLWA010000029 DAVLWA010000000
VERSION     DAVLWA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.029"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            92..1021
                     /locus_tag="VYN80_001037"
     CDS             92..1021
                     /locus_tag="VYN80_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001150386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1540963.1"
                     /translation="MQDESIYGLVYKTIREERGFTQKEAAGTTVTPHFLRQFEQGKSR
                     ITIQKFEEILSNIGIDKETFDSLKTQMFPTEFHKLQAQVADLAYKREFKKIISLINQP
                     MENPGIAEHYVIANRVVNKFAIANIIGDSFLTPKDYQELEYIKNYLSELDDWNKIEVN
                     IFSSILPHFSIEFLDYRLYHLLDTLKKQTEYHSIRTADYYIACLRTAIKHYSVNGYYD
                     KAESLAVKTLEVINTFPLLSTKMTEMISLSMERANNFLRKDDVRGLELAKHIFASLDN
                     FEKVYPNQLLTRMREDFFVTVTQLNHTGQPLDV"
     gene            complement(1071..3407)
                     /locus_tag="VYN80_001038"
     CDS             complement(1071..3407)
                     /locus_tag="VYN80_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1540964.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLGPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGAALNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESRVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQVDQD
                     NDVNRIIEQLEEQTLESHKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKILQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQVQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(3511..4059)
                     /locus_tag="VYN80_001039"
     CDS             complement(3511..4059)
                     /locus_tag="VYN80_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1540965.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4056..4358)
                     /gene="zapA"
                     /locus_tag="VYN80_001040"
     CDS             complement(4056..4358)
                     /gene="zapA"
                     /locus_tag="VYN80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1540966.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            4445..5326
                     /locus_tag="VYN80_001041"
     CDS             4445..5326
                     /locus_tag="VYN80_001041"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1540967.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVITRDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5330..5944
                     /gene="lepB"
                     /locus_tag="VYN80_001042"
     CDS             5330..5944
                     /gene="lepB"
                     /locus_tag="VYN80_001042"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1540968.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            6072..8438
                     /locus_tag="VYN80_001043"
     CDS             6072..8438
                     /locus_tag="VYN80_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1540969.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(8486..9769)
                     /locus_tag="VYN80_001044"
     CDS             complement(8486..9769)
                     /locus_tag="VYN80_001044"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1540970.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(9963..10322)
                     /locus_tag="VYN80_001045"
     CDS             complement(9963..10322)
                     /locus_tag="VYN80_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1540971.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(10621..11250)
                     /locus_tag="VYN80_001046"
     CDS             complement(10621..11250)
                     /locus_tag="VYN80_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1540972.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVCYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGFPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            complement(11295..11534)
                     /locus_tag="VYN80_001047"
     CDS             complement(11295..11534)
                     /locus_tag="VYN80_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540973.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(11684..11950)
                     /locus_tag="VYN80_001048"
                     /pseudo
     CDS             complement(11684..11950)
                     /locus_tag="VYN80_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(11975..12286)
                     /locus_tag="VYN80_001049"
     CDS             complement(11975..12286)
                     /locus_tag="VYN80_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1540974.1"
                     /translation="MDWDFADTCWGKEPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(12211..12442)
                     /locus_tag="VYN80_001050"
                     /pseudo
     CDS             complement(12211..12442)
                     /locus_tag="VYN80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(12443..13075)
                     /locus_tag="VYN80_001051"
     CDS             complement(12443..13075)
                     /locus_tag="VYN80_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1540975.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(13089..14084)
                     /locus_tag="VYN80_001052"
     CDS             complement(13089..14084)
                     /locus_tag="VYN80_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1540976.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(14081..14779)
                     /locus_tag="VYN80_001053"
     CDS             complement(14081..14779)
                     /locus_tag="VYN80_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1540977.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(14857..15867)
                     /locus_tag="VYN80_001054"
     CDS             complement(14857..15867)
                     /locus_tag="VYN80_001054"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1540978.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQDWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(15851..16858)
                     /locus_tag="VYN80_001055"
     CDS             complement(15851..16858)
                     /locus_tag="VYN80_001055"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1540979.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(16845..17798)
                     /gene="mvaD"
                     /locus_tag="VYN80_001056"
     CDS             complement(16845..17798)
                     /gene="mvaD"
                     /locus_tag="VYN80_001056"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1540980.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(17780..18658)
                     /gene="mvk"
                     /locus_tag="VYN80_001057"
     CDS             complement(17780..18658)
                     /gene="mvk"
                     /locus_tag="VYN80_001057"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1540981.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSYGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(18780..19109)
                     /locus_tag="VYN80_001058"
     CDS             complement(18780..19109)
                     /locus_tag="VYN80_001058"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1540982.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLTVWSFFRRSMKTGWFQINGKWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         5592 a         4361 c         3627 g         5677 t
ORIGIN      
        1 aagcgtaact ataattactt ttttctaaaa attcccttaa aaactggtag aattatgata
       61 gaatagaaaa aaggattcga ggagatttag gatgcaagat gaaagtattt atggattagt
      121 ctataagacc atccgtgagg aaagagggtt tacacagaag gaagctgctg ggactacagt
      181 gactcctcat tttttacgtc agtttgagca agggaaatct cgaataacca ttcaaaaatt
      241 tgaagagatt ctttctaata ttggaattga taaagagacc tttgatagtc tcaaaacaca
      301 gatgtttcca acggaatttc ataaattaca ggctcaagtt gctgatttgg cctataagcg
      361 tgaatttaaa aagatcatta gtttgattaa tcaacccatg gaaaatcctg ggatcgctga
      421 acactatgtg attgccaatc gtgtagtcaa taaatttgct atcgcaaaca tcatcggcga
      481 ttcttttctg actccaaaag attaccaaga actagaatat atcaaaaact atctgagtga
      541 attagacgac tggaacaaaa ttgaggtcaa tatcttttca tctatcctgc ctcacttttc
      601 aatcgaattt ttagattata ggctctacca cttattagat actctaaaaa aacagactga
      661 ataccattca atccgtactg ctgactacta tattgcctgc ttaagaactg ctatcaagca
      721 ttactctgtt aatggctact atgataaagc tgagagctta gctgtgaaaa ccttggaagt
      781 gattaatact ttccctctac tgagcaccaa gatgacagag atgattagtc tttccatgga
      841 acgggctaat aattttctca gaaaagatga tgtcagaggt ctagagcttg ctaaacatat
      901 ctttgccagt cttgataact ttgaaaaggt ctatccaaat caacttctga ctcgaatgag
      961 agaggatttc tttgttactg tcacacaact caatcacact ggacaacctt tagatgtcta
     1021 acctagaaaa taaaaaagct tgaggaacca catatttcct caggcttttc ttatccttta
     1081 aaagtgacaa tagtcgcgcc actgcctcca gcattttgtg gggcatagcc aaaactcttg
     1141 acatgtttgt ttctttgtag gtatttggtg actccttcac ggatgacacc tgttccgata
     1201 ccatgaatga tatcaacttg agccatattg ttaagcaggg cttggtcgat aaaggcatct
     1261 agctcattca tggcctcttc atagcgtttg cctcgaaggt ccagtctggc ttgtggacct
     1321 cggccagatg tccgtttaac aacattgact tgtttcttct taactggctt ttcttgctga
     1381 acttgaacaa ggtcaaactc tttctcttcc agggtcatct taatcaagcc aacttgggct
     1441 tcccagcggc cgtccttgag ttgactggtc agggtaccac gctggccata actgagaacc
     1501 acgatatcat ctcccacctt tggagctcgt tttttcttgg ccttttgaag gatcttgttt
     1561 ttagacaaat ctactttttc aggagccaat tttttcagct tggccttggc ttcaatgatt
     1621 tcgtggggct tgagttgaga tttactgtgg agatttttga gaatctggtc actctcactg
     1681 agggccatgt ccacaatctc agcagcctgt tcacgcgcct tattaagctc ggtttccttt
     1741 tcacgattga gttcgttgta aagttttttg agagcacggt tcatcttgag attttcttgc
     1801 tccacctcac ggatattgtc caagcgttta tgactttcaa gcgtctgctc ttccaattgt
     1861 tcaataatcc gattgacatc attgtcctga tcgacctgct ggctggcatc ccctacgata
     1921 acttcagata ggcctagacg tttggcaatt tcaaaggcat tacttcggcc aggaacaccc
     1981 tgcataaagc gataagtcgg gcgaagagtt gcagtatcaa actccatact agcattttgc
     2041 acaaaggctg tctcaatacc gtaagccttt agttccggat agtgggtagt cgccatagtc
     2101 ttgatttgac gcaggcgaag gtcctccaga atagccatgg caagagcagc tccttcttga
     2161 gggtctgtac cagcccccaa ctcatccaag agtaagagtg aatgttgatt gaccttgcca
     2221 agaatatcca cgatattggt catgtggcta gagaaggtag acaagctctg ctcaatagac
     2281 tgctcatctc caatatcggc aaagatttct tcaaagatac caacacgact tcccttatct
     2341 gctaaaatcg gcaaaccgga ctgagccatg acctgtgtca agccgagagt tttaagcatg
     2401 atggtcttac cacctgtatt gggacctgta atgacaatag ctgttaaatc ttgaccaaaa
     2461 tggacatcat ttgcgacagc atttttgacc aaaggatggc agacatggag cagttgaatc
     2521 tcttgatttt ctgacagctg aggtacgact gcttgccttt cttggataaa gcggaccttg
     2581 gcacgaatca agtctagatg accgataatc caggcgtcat tggcaatctc agccgcatga
     2641 gggcggacac gctcagaaat ttcttggaga atgcgaagca tttcatagcg ctcatctgct
     2701 cgcagactag caatttcttc gctcagtttg actacctcac gtggttcgat atagacggtg
     2761 tttccactag cagaaatatc atgaacgaca cctgcaatct tattgcggta ggtgtttttg
     2821 actggtaaaa cctgacggcc atttctgcta gcaacaattc cttctgtcaa catctgcgct
     2881 ttttgcttga gcaagtcttg taaaacatcg cgtacccgac tctcgctatc atgtattttt
     2941 cgacggattc gcgccaattc ttcactggca aaattttcaa tgaaacccgc atcattaaag
     3001 gcctgaagat ttccttgtaa ttgcggaaaa tcatgtaatt tctcaaacca aagggctaat
     3061 tcttccaagc tgacattttc cagattggcg taaaaacttt gaagttctcg gctggcaaga
     3121 agcacgcgtt tcaagagtag gaactcctcg atattgagag ccgctcccat ctccaacctc
     3181 ttgcagactc ctgcaatttc cttagttgag agaatagtaa aatgcggttg ctcgacgaaa
     3241 agagcctgca tttccttcat ctcagcaaaa gcctgtttga ttttatctgc tttggcagtc
     3301 ggacccagct gtctcaattg ctccaagccc tgctcggtca acaaatgagg ctcaaacaag
     3361 gccttgacct tatcgaactc taatgtttct aatattttct tattcatctt tatttccttg
     3421 tctttgaatg tctaggtgtg gtagcttttt acattctgat agattcgagt caaatctgta
     3481 aacaaagggc taggaaaact cctgcccttt ttatccgatt aaatttgtca cccagatttg
     3541 tttgagccaa ctggttgtta tcggtatgct ctggatgatg tgttttgcga cgatactctt
     3601 ttcaagagga ttttgtataa ctgccatggg gatggtcgcc aagattgtca aggccatttg
     3661 caagacaaat aaggtcacca acatggacaa gatacctgct gaaacttgga acaacttacc
     3721 acccagtttt ttgctaggaa tcaagtgtaa gagaagacca agcaaacgac caatgctata
     3781 gacaatccca aatacaagca agtagccgat gcctgcataa aagaccttat ccagctgaaa
     3841 gagttgatcc gatgggaaaa agaaagtccc ctgaccttcc tgcgaatttg cataagggag
     3901 caataaatgg aattgctctc caagcccctt ataaaactgg ccagccataa aagccgatgc
     3961 catggctgaa atcaggtaat aaacctgtaa gagcaggcct ctccgatagc cgatataaaa
     4021 tccccaaacc aagaccaata gaagaaggaa tgaaatcata aggaatcctc aatcttgctc
     4081 tgttcttgct tacaagtcac aagcttgtga cggagttctt ctagctcttg ctccttatcg
     4141 tcaaattcaa tctcacggct gagctgagtt gataaacagt tgactgccaa caaaagagcg
     4201 attgtttcat catctgcgct aggcatttgt tctttaattg cttggtattt ttctgtcgca
     4261 accttagcga tttcctccat aaaaaggtta tcatgttcgc ttgtcaaggt taacgatttt
     4321 ttcccgaatg taaatttgaa tcgatttaga tttgccataa aattcacctc acgatattat
     4381 accaaaattc gctaattttg tcagttttta caaattttac tgcttttgtg gtacaataga
     4441 aactatggca agtatcacac tcacaccaag cgaaaaggat attcaggctt ttcttgaaca
     4501 ctatcaaacc agtctggctc ctagcaagaa tccctatatc cgctactttt tgaaactacc
     4561 tcaagcaacg gtttctatct atacttctgg aaaaatcttg cttcagggtg aaggggctga
     4621 aaaatacgcc agtttctttg gctatcaagc tgtagagcaa accagcggac aaaatcttcc
     4681 tttaattggg acagatgagg tgggaaatgg ttcctacttt ggtgggcttg cagttgtggc
     4741 tgcctttgtc acacctgacc agcacgactt tttacgaaaa ctcggtgtgg gggattctaa
     4801 gactctgacc gaccaaaaga tccgtcagat tgctcctatt ctcaaggaaa aaatccagca
     4861 ccaggcactc cttctctcac ccagcaagta caacgaggtc atcggagacc gctacaatgc
     4921 tgtttcggtt aaggttgccc tccataatca ggctatctat ctcctccttc aaaaaggtgt
     4981 tcagcctgag aaaattgtga ttgatgcctt taccagtgct aaaaattatg acaagtactt
     5041 ggcacaagag gccaatcgtt tcagcaatcc tatcagctta gaagaaaagg ctgagggcaa
     5101 atacttggct gtcgcagttt cttctgtcat tacgcgtgat ctctttctgg aaaatcttga
     5161 aaatttggga cgagaactgg gttatcagct tccaagtgga gctggaacgg cttctgacaa
     5221 ggtggctagc cagattttgc aagcctatgg tatgcaggga ctcagcttct gcgccaaatt
     5281 gcactttaaa aacactgaaa aagcgaaaaa acgcttagaa aggtaagtta tgaattcatt
     5341 taaaaatttc ttaaaagagt ggggattgtt cctcctaatt ctgtcattac tagctttaag
     5401 tcgtatcttt ttttggagca atgttcgcgt agaaggacat tccatggatc cgaccctagc
     5461 ggatggcgaa attctcttcg ttgtaaaaca ccttcctatt gaccgttttg atatcgtggt
     5521 ggcccatgag gaagatggga ataaggacat cgtcaagcgc gtgattggaa tgcctggcga
     5581 caccattcgt tacgaaaatg ataaactcta catcaatgac aaagaaacgg acgagcctta
     5641 tctagcagac tatatcaaac gcttcaagga tgacaaactc caaagcactt actcaggcaa
     5701 gggctttgaa ggaaataaag gaactttctt tagaagtatc gctcaaaaag ctcaagcctt
     5761 cacagttgat gtcaactaca acaccaactt tagctttact gttccagaag gagaatacct
     5821 tctcctcgga gatgaccgct tggtttcgag cgacagccgc cacgtaggta ccttcaaagc
     5881 aaaagatatc acaggggaag ctaaattccg cttatggcca atcacccgta tcggaacatt
     5941 ttaagaaacc taagaggccg agaatcacca atctcagcct cttcttctat cgtgagaaaa
     6001 tgattggtaa ctatctaaac ttaccagaac agaaacacct caactctcac ctattcatgc
     6061 aaaggaattt tatggaagtt tatttttcag gaaccattga acggattatt tttgaaaatc
     6121 ccagcaattt ttatcgcatc ctcctcctag aaatcgacga tacggacgca gaggattttg
     6181 atgactttga aatcattgtc acaggaacca tggctgatgt aattgagggc gaagactata
     6241 ctttttgggg gcaaattgtc cagcactcca agtatggaga acaactgcaa atcagtcgtt
     6301 atgatcgcgc aaaaccaact agtaagggct tggtcaagta cttttcaagt agccatttca
     6361 aggggattgg tctcaagaca gctcagaaaa tcgtggatac ctatggcgaa aataccattg
     6421 acgaaatttt gcaacaccca gaaaagttag aaggcatcgc aggactctct gccaaaaatc
     6481 gcgaggcttt cgtctccact ctccgtctca actacggaac ggagatggtt ttggccaaac
     6541 tagccaacta cggcattccc aacaaactag cctttcagat tcaagacttt tacaaggaag
     6601 aaacccttga tgtggttgaa aattatccct accagctggt tgaggatatc aagggtttgg
     6661 gctttaccat tgctgaccaa ctagctgagg aactaggcat cgaaagtcag gctcctgaac
     6721 gcttccgcgc cggtctagtt cacagtcttt ttcaggcctg tatggaaaca ggggacacct
     6781 atgttgaagc acgggatttg ctggaacaaa cccttactct ccttgagtct tcccgtcccg
     6841 tggaactaga ccccagccaa gttgcccaag agctctccta cctgatcgaa gaagacaagg
     6901 ttcagcagat tgataccaag atctttgaca acagcctctt tttcgctgag gaaggcatcc
     6961 gcagtcactt ggttcgtatc cttgaaaaag gaaaacagaa gagtcatgat ttaaaaacca
     7021 ttcaaaaaca tatcactact atcgagcaag agcttggaat tgagtatgat aacattcaaa
     7081 aacaggctat ctgtgatgct atccagaata aggtctttat cctgacaggt ggacctggta
     7141 ctggtaagac aactgttatc aatgggatca tcgctgttta cgccctgtta gagggacttg
     7201 acttcaggaa gaaaagcaac ctgcccattc ttcttgctgc tccaactggt cgagctgctc
     7261 gtcgcatgaa tgaattgaca ggtttgccta gcgcgaccat acaccgccac ttgggaatga
     7321 caggtgacga tgataccagt catctggaag attacctaga tgctgacttt atcatcgtgg
     7381 atgaattctc tatggtggat acttggctgg ccaatcaact cttctccaat atctcttcta
     7441 acagtaagat cctcatcgta ggtgacagtg atcagctacc ttctgtcagt cctggacagg
     7501 ttctagcgga tctgcttcat attcctttga ttcctcagac tcgcttggaa aaaatttatc
     7561 gacaaagcga agaatcaacc atcgtcaccc tagctagtca gattcgacag ggcatcttgc
     7621 cagctgattt cacccaaaaa aaagctgacc gttcctactt tgaaattgct agtggccata
     7681 ttcctgccac cattgaaaaa atcttaggtg ccgccctcag aaatggtatt cctgcccgtg
     7741 atatccaagt tctggctcct atgtaccgag ggacggcagg gattgatgct atcaatcagc
     7801 tcatgcaaga cctgctcaat cccccacaaa aagatcaact cagttttgaa gctcctcagt
     7861 gccactatcg taagggagac aaggtcattc atttggttaa cgatgctgaa atcaatgtct
     7921 ttaatgggga tttaggagct atcacagacc tgattcctgg taaatacacc gaatcgaaac
     7981 aagacgagat tgtcattgat tttgatggta atgaagtctc ttacccccgt aacgaatggt
     8041 acaagattcg cttggcctat gccatgagta ttcataagtc tcagggaagt gagttcccag
     8101 ttgtcatcct accgattact agtgctagcc gacgtatgct ggagcgaaat ctcatctaca
     8161 cagccattac acgtgccaaa agcaaactca tcttactagg cgaattacaa gccttcgact
     8221 atgctaccca acacatcgga actgcccgaa aaacctatct gattgaacgc ttcagtgatc
     8281 tactggagaa tgttgaagaa aagcaacaag ctgtctctga aacagtcaca tcaagtgcct
     8341 ctgaacaatc ctacatccta accgaagaaa actgggacag cattccagcc atgattggga
     8401 ttacagacac agacctcaaa gagatttttg gaaaatagta catcagaaaa cccaccaatt
     8461 caggtgggtt ttctgtttat taagattatt ttactgttgc tgtgcttgtg atcaattcaa
     8521 cagctttttt gatagtgata tcatgtttca acatgtcagc tgaaagcaag ttttgaactt
     8581 gtgcaacttc catgttgtag tctgctgcca attgctcaac ttctttttgg atttcttctt
     8641 ctgaagcatc aaatccttca gctttggcaa ctgcttcgat aacaaggtta gtcttagtac
     8701 gtgactcagc ttctgcttgg tattggttgt gaaggtcttc ttgagtagtt ccagtgattt
     8761 ggaagtacat gtcagggttg atcccttgac gttgcaagtt cccaaggaat tcatttactg
     8821 aacggtgaac ttcttcatgg atcatttctt ctggaagttc tacgatttca gcgttttcta
     8881 cagctgtatc aattgctgca ccttcaactg catctttgta agcttcttct ttagcagcag
     8941 ccaattcttt gcggtatttt tctttcaagt cagcaagtgt ttcaacttct tcatcaatgt
     9001 cttttgcaag ttcatcgtca agagctggaa cttctttagc ttttacttcg tggatagttg
     9061 tcacgaattt agcttcttta cctgcaaggt cttctgcttg gtagtcttct gggaatgtta
     9121 cgataacatc aacggtttcg ccagctgagt gacctaccaa ttggtcttcg aaaccaggga
     9181 tgaattgacc tgaaccaagt ccaagtgaga agttttcacc ttttccaccg tcaaattcaa
     9241 caccgtcgat agaaccaacg aagtcgatca caacagtgtc gccgttttca gcagcagctt
     9301 ccttgataac caattcagcc agattgttgc gttcgcgttc gatacgctct tcaacatcag
     9361 cgtcagttac ttctttttct acatcaactg atacttcaag gtttttgtag tcacccaatt
     9421 ttacttcagg ttttgtaacg acttcagcag cgataaccca gtcttgacct ttttccattg
     9481 aagttacgtc aatttttggt tgggcaacca cttcaagacc agcttctttt acagctgctt
     9541 cataagcgtt tggcaaaaga gcgttcataa cgtcttggta aagtgattct tcaccaaatt
     9601 ttttatcgaa gataggacgt ggaaggtgac ctttacggaa acctggaaca ttaagagatt
     9661 tcttcactga cttgaagaca cggtccaatt ctggtttgat ttggtcttga gagatagtga
     9721 aagtcaagac accacggttt gtttctttgt tttcaaatga tacagacatt ctgtcatttc
     9781 tccttaaaat tttttaaata cagtctatta taacattttc tataaataaa atctagacta
     9841 atgcagacaa ttatagacaa caagcctttt tcctaactaa aacactcata aagtatagac
     9901 aattttagac tactttggat atcttttggt tagtcatttt ccaaaactaa tttatctaat
     9961 ttttaagtta tatttgactt catcttctca gtaacatgag tataaacact taaaatcttt
    10021 gtttcattag tataatctac acgatccatt atcgctatta taggtacttc caattcagca
    10081 agcaaactta tatgcgaata tctaaaaaca tgagcagtca atacaaaatc atctttctta
    10141 atttcacttt tcctaaacct gtttttcaaa aagtaattga aacaagttat cattaaggga
    10201 gaaccattat ttgtaaatat ataatcacct tcttctaaaa gaagatattc ttttaaaatt
    10261 tctaacactc tatcactaat accaattact ctatatgaag ctaaactttt cggactatcc
    10321 aaaaacactt cgttattgga cgattacatt atagtaaaac gaaataagaa caggacaaat
    10381 cgatcaggac agtcaaatca atttctaaca atgttttaga agtagaggtg tactattata
    10441 gtttcaatct actatacaag acgaaattga agaaatcttt ccattataaa acgcatatta
    10501 tcaaggtttt taaacaccta ataatatgcg ttttttccta ttttaaagac ttttatccac
    10561 ctattataca aagttaagta tccatcacag tttacaatta gccttcatta cctaacccat
    10621 tcaccatcct tcaagagagt aacctttaag aaccgaataa ataaatgaca aatttcgttc
    10681 atttaattta actgcactgt taatttgatt agctccatct cctaaagtat gcactcctac
    10741 tacacggtga ctagcatcat aaactgtaga tccactagac ccctctaaag tatcaacttg
    10801 gtaatccaag aacatgccat catcacttaa aacttgtttt ttatctgtat acatttgatg
    10861 aattttaaaa ttatatgatg gatagcctgt gatagtcaca gttattcctg tcaaattttt
    10921 ttgactagta ggaaaaccca aagtccctaa ttttgcacca atgggctctt ctagaattaa
    10981 taaagccaag tcatattccc ttgcatcctt agaatttaaa tttctaaatt ccttcaaata
    11041 acaaacttcc tttactttga tctttccaaa tagttcttga cttggactaa cagccggaag
    11101 aacataaata tcatccgctt ctttcccata gtcatgtctg tagtagttgt gagccgctgt
    11161 aattaacaca ttatctttaa taaaattcgc acttcccctc ccttctacat tcattccatc
    11221 ggctactcca taatacttag ataaaaccat agctgaagtc gaatacggat actgtaaagt
    11281 attatcaatt ttaatcaaat catcattacc gataatactt ctgattgctt ttggtagtat
    11341 gaaccatacg ttggtgaaat ctcagataat gaagaatcat tagaccctgg acctttttct
    11401 agtgtctcac ttacctcata ttcttcacct gtactagaaa taacactcaa agcagatact
    11461 gtcgataact ggctagccaa taaagtactc gcaataattg aaatacccaa ttttttataa
    11521 acagttttct tcattattgt atcctcctaa tgtaattata gcgtactatt ctaaatttct
    11581 taatctacta tagaatcaag aaatctacca ccttctttaa ataccctcca ttatcacata
    11641 aacaggtaaa cttttcaatt aatgactgcg cttttcaatc acgctagagg tacttacttg
    11701 cttctttgat actaagttca gccattcttt ccttgttttt ctcaataaag catgttaccc
    11761 aagtgggatt cgttttggag tagtctcgca gagtccagcc aatggcttta ttgataaaaa
    11821 attctgtttg gttcaagtta tgaagtagaa tcttttccat taattgagta ttggtcttct
    11881 cttttcttaa caactggtgg tcaatagcga cacgtctcag ccagatatta tctgataggc
    11941 tccattttat actcaatgaa aatcaaagag caaactagga agctagccgc agattgctca
    12001 aaacactgtt ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta
    12061 ttaagattat ttcttctagt tcagggtgtt catacaccaa actccctact actcgatcta
    12121 ggatatctac cgtgtcccac caggattttg tcacgactaa ctgctctagc ttaggcaaat
    12181 cggtttcctt tagataagac tgcattgctt tcaaatagtt agcagccaca tattggtatt
    12241 ctctaggctc ctttccccag caagtgtctg caaaatccca atcgataatc tttgtttttt
    12301 tcgcttctgg aaaatatttt tatagagttt atttcttgca acctagaaaa gaaaattgat
    12361 ggcgcatata ggcttccatg ggcattgctt ttttagagtc ttttgctgct tctagctcct
    12421 caagtaaatc tgctaaactc atctaaaact cctcttgccc caccaaatgg tgctgaaagg
    12481 catagacagc cgcctgggta cgatcgctga cttcaagttt ggcaagaatg ttggacacgt
    12541 gggtcttgac cgtcttgaga gagataaaga ggtcatctgc gatgcgctga ttttcgtagc
    12601 ccttggcgat gagttggaga acatctcgct cacgcgcagt caattcttca tgaagttcca
    12661 tatgattgcg gtggtattca accttcttgc taacctcttg ctcaatggcc agctcgccag
    12721 cagctacctt actgacggca tgaagcaatt catctgcact agaagtcttg agcatatagc
    12781 ctttggcacc agcatctaag actggcatga ttttttcatt gtccaaataa gaggtcacaa
    12841 tcaaaatctt ggcttcaggc cattctttaa ggattgctaa ggtcgcgtca atcccattca
    12901 tctcaggcat gacaatatcc atgacaatga catctggacg cagttccaag gccaagtcaa
    12961 tcccttgaga cccgttggac gcctcaccca caacttctac atcgtcttgg aggtcaaagt
    13021 agcttttcaa gcccaaacgg accatttcat ggtcatctac tagtaaaatt ttcatcttta
    13081 ctcctttatc attccttatc taacagggga atacggatat caactgccag cccttgcttg
    13141 ggagctgtta ataactgaac cgtccctgcc atatcttcaa cccgctcctt gatatttcgc
    13201 agtccataac tcaagtcgtc taagctccct aactggaaac caatcccatt gtccaccacc
    13261 ttcagttgca attcaacatc tgtctgatag aggtagacat ctaggcaaga tgcctgggca
    13321 tggcggaggg tattgctaat caactcttgc aggatacgga agatatgctc ctcgattttc
    13381 ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc
    13441 tcttttaaga gaatctgaat cccttctatc aagctcttct gctccagttc aactggtcgc
    13501 aaatgcaaga gcaaaacccg caaatccttc tgggcagttt ctaaaatagc tgtgacactc
    13561 tgcaactgaa tctgcatctt ttctctatcc aatttcaaag cctgctgact gatacccgat
    13621 aaaatcatgt gggccgcaaa caactcctga ctgactgtat cgtgcaaatc ccgagcaatt
    13681 cgcttccgtt ctttctcgat gatttcctct tcctgagcaa ggctatgatt ttcagctttt
    13741 tgaagagctt ctgtcaaaag gttaagttta cctgataagg acttgaaact ggcatccaaa
    13801 tctggatctg caacctgaac cacttcttgc cctgctaata aacgcttgag attagcctgc
    13861 atttttctta gagaaagctc ttcgatccct cgccaaaaca gggctaagag acaggtcatg
    13921 gacatgctga aaaccaacaa taaaaagaca aatttttctg ttttttcgac atcgtgcaaa
    13981 aagatagacc agtcaaaatc aagtatttcc agcaagctgt gggagaaaaa aaagacaaac
    14041 aggaaggagg tgagagcaat aatgacatag gcttgttttt tcatcctcta accacctcca
    14101 catcaccaat catagtggtc aagaaaatct tgacactctt gttactcttg agatagtctt
    14161 ttgtttcttg atgatagtgt tcattgcgga gggctcgctt gggctggttg aaaaaagtca
    14221 aatccccata gagacagtta acgctgagac tgacttccac atctacaggt acgatgattt
    14281 tggtcgttcc taccatcttt ctgaggataa tgacattgtc atgattggtt aagatgaccc
    14341 tctccagatg aatagtgtcc ttgcccatga agcgaaagag attgatatca tcgaattggc
    14401 aagtctggta gcttgaaaaa tgatgaagat ttccaaacca acgatttttc tccttcttaa
    14461 ccgtcacgac ctcttcaaaa accaaattgg tctgctcttt ttcctggttc atcatcgggt
    14521 aaagaagaaa gaggctatag ataaccgcaa caaaaatagc tagaatcaca aaaggattga
    14581 gcataacgat gaaaaagaag agaatggtta ccgctactaa aagaagatta tttccctctt
    14641 taccagtgta gtagcgaatc aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa
    14701 aatgctctga taccatcaaa atcagagctc ctgtcagaag acaggcttcg ataaataaaa
    14761 agattttaaa ttttctcata ggttcatcct ctcccttcta ttttatcaca attcaaaaaa
    14821 gtcacctcag tctgaggatg gaaaaaaagg cgctggttac gcctttttca tctgatcctt
    14881 tgcttctttt aattttccat aaagaagata gtctactttt tgtagatctg ctatggtggc
    14941 acagttaagg gaacacataa tcaagcgtag atctgctttc cagtcttgga caatgccaat
    15001 cacttcttca actgtgtagg tttcaaccaa ttccagaacg gttcgtgaca atcccacaga
    15061 cttagcacca aaaaccaagc acttaatcat atccagcgga ttccgaaccc ctccactaac
    15121 caagagttcg accttatctt tccattcttg ggcattgaga agggcctgca tggtagactg
    15181 accccattga ttgaggtaat cacgctggcc actacgacgg ttttcgatat aggcaaagct
    15241 ggtgccacca cgacccgata ggtccactgt acgaacaccg aattcatagg ctctttcgat
    15301 tgtcttggca tccattccaa agcccacttc cttgaggaca ataggaacgg gaatttgctt
    15361 gctataatct gctagatgcg attgccagct tctaaacttc ctttctccct cgggcatgag
    15421 taattcctgc atgacattga catgcacttg caatagaaca ggattcatct cttctacagt
    15481 ctgaagtcct aactcgacag gcttgtccaa tccaatattg gttccaagga ggagattggg
    15541 atgactagac ttgacagaaa aagaatcatc cgttggattt ttgagggctg cgctataaga
    15601 acccgttaca aataaaatac cacaggattc cgccacctga gccagctttt gattgatttc
    15661 tcttccctta ttacttccac cagtcatggc attgatataa aaaggaaagt cccactttcg
    15721 accagcaaac tctgtcgaaa gatcgatttc atccagattg taaagaggca aggaagaatg
    15781 aatcagctcc acctcatcaa agctattata ggaacttttc tgctcaaggg catagaggat
    15841 atgctcgtcc ttacgatttg tcgtcatgtc ctatcctttc ttgatataag agctcaatcc
    15901 ccagatcggc ccaacgattt tttaaggttt tggttgattg cgcatcaaaa ctcagggcga
    15961 tgccacagtc accaccacca gcaccactac tcttggcaac ggcctgcaaa tcttgactgg
    16021 cttctttcaa ctgtctaagc aaaggcgtgt aaatatctgt actcaagcct tctaaaagct
    16081 tgctggctac ttctacttgc tcgataactt tttcggcttt cccctgctcc aaggcttcga
    16141 ccaaagaaac caccgtttct tttgaggaac ttaaaaaatt ttgattgata ttttgcttga
    16201 tttgctggac catgtgactc gatacagcca cttccttggt ccatcccact aagaaatcac
    16261 attctaaagt tggtttcact tgtgagataa aaaatcccca atcacgctcc agaactgtcg
    16321 ccaagttttc ttcttctaac caagcagccg ccttctggcg atcaaatgac tggtagagaa
    16381 ccaaatcctc tgccacaata caggcaaggt cgcccatgga accattgtct cctcgcttga
    16441 gcaagacagc gctagtcagc ttgaacaaga gattctgatc aaccgaaaga ttatagagag
    16501 ccagtaaagc cttgacaacc aagacaacga cgctgccact agaacctaga ccaaactttt
    16561 tcccttctcg ttccattttg ccacagattt ttagggaaaa aggtcttaaa ttctgaccgc
    16621 gaacagcgag gaagtctccc atcaaagcaa tcgtttcttg aatcaagctg tagtcaggat
    16681 taggccttaa gtccactgcg aaatcaaaca tatctgaata gatacggtag ctgtcagaaa
    16741 aagcaatctc agccctcata tagatgggaa tatcctttat caaagctaac tgccctggct
    16801 ctaaaatagc atattcacct gcccaataga gttttccgca agttttaaca gcaatcatct
    16861 tgactcaaat cctttgtttt tgacacaatc aagcgataac gatgaccgaa aatttctgat
    16921 aaatgctcca agtctttctc ctgacagaag accttaacat tgggaccagc atccatggta
    16981 aagtagcagg cctctccttt ctcacgaagc tggcgaacaa agtccatagc ctcataagag
    17041 gcatccgtca gataagaaaa ggctggacta gcagtctttg tcgtagcatg catagccagg
    17101 gcatttttct ccgttaattc tccaatcttg gcaaaatcat tttccttgag ataaatcagc
    17161 atatcctgat agtccttctc agactgacga acccagtcgt cgaaagtcgt cgaggtttcc
    17221 acacaaagtt tcatcccgtc acggctagag attggttttt tcttgtcctc tagcaccaac
    17281 ataatcatag ctagtttcaa gtctgtctct acagggtaaa tttctccact atccttatcc
    17341 caggctccta gtggtccata aaaactccga gaagaagagc ctgaggcaaa cttggcttcc
    17401 tgcgctaact gacttctatc caatccaagc ttgaaataag cattacaagc cttgaccagg
    17461 gcggacaaac cactagaact tgaggacagg cccgctgccg taggcatatt gttttgagta
    17521 tcgatacgga caaagccctc accagctgga cgataacggt caataatctt actcatcttg
    17581 gcatgctcga cctcattttg tagctgacca ttgatgtaaa attcgtcagc tgttacattg
    17641 gctggtaaag gcgacaaggt cgtctctgta tacatatttt ccaaagttag agaaatactg
    17701 ctagtagcag gcaccatctc tttttctttt ttctttcccc aatatttgat aatagcaata
    17761 tttgcgtagg aacgtactgt tacaggctct ctatccatgt ctgaacagct cctttctctt
    17821 ctaatctttc tgctagttct tgtgcgtgtg tcaaattggt taccaaggct atgatacaac
    17881 ctcctagccc accaccgctc atcttggcac ccagagcacc atagctaaga gtcgtttcaa
    17941 ccaaaaagtc tgcctcaggg ctactgactc caatttcttt tagatgtaaa tgcgcttgac
    18001 tgaggatttg tcccagtcct tcagcatctt tttgtgaaat cgcaacttct gcttgctggg
    18061 ttaattctcc caaggcatgc aaaaacggta gggcatcctt gcccttattt tgaaccactt
    18121 ggatggcttc acgagtatga ccataaacac ccgtatcggc aatcaccaaa taggcggata
    18181 aatccatctc aagttctgta aatcctacgt tcttgataaa gcgaataggt tggtcactga
    18241 gacaggtctt agcatccaaa ccactaggat tcatatgggc aatcatctca gctcgattga
    18301 ccaagatttc tagtacatca tgaggcagat cagcctgata gtagtcaaat accgcacgaa
    18361 tggccgctat gctgatagcc gctgacgaac ccatcccccg tttctcaggg atagccgagt
    18421 caatctcaca acgaacgcag gcttctgtga tatccaaata ctccagtgaa gcataaaccg
    18481 ccatggacaa ggtatcctcc tcataaaggc gccaaggact ctctgcagga actaccttac
    18541 aggtcacctc cacctccaaa agaggcaggg aaatggcagg ataaccgtaa acgaccgcat
    18601 gttcccctat taaaattatc ttactatgtg cctgaccgac accaactttt tttgtcattt
    18661 tttcctttta ctagacgaaa aaacgtctta tttttcatac aagtattaat tctttcctat
    18721 ctattttatt atattttcac aaaaaaagcg attgtttcca ttcacaatcg cttctttcat
    18781 tattgaaccc attcgccatt ataattgaca gaatagccat ctacggtcgt attcactgcc
    18841 aaagcacctg agctataagc atagtaccat ttgccattga tctggaacca acctgtcttc
    18901 atagaacgac gaaagaaact ccataccgtt aagtaaagag gaaagtcgtg agggagcatg
    18961 cgccattgac aacctgtttt agtgacgtac aaagtctcat taacaagtac tcgtttcggc
    19021 catttatagg tgcggtgttt ggagaaatag ggttcaatct tagcccattc ttgatcgttt
    19081 aaatcagtat catatgcttt gcgtgtcata actctagttt aacatttttt tgtgaataca
    19141 ggttctaaat aatcgaccac gaaaatttct taagtggaaa acgccttatg aagtatgcta
    19201 cgggaaagtt atgcacttaa tttgacaatt caagatgtaa aaatatatac tatatta
//
LOCUS       DAVLWA010000030        19140 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLWA010000030 DAVLWA010000000
VERSION     DAVLWA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.030"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            33..1514
                     /locus_tag="VYN80_001059"
     CDS             33..1514
                     /locus_tag="VYN80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1540983.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1876..2877
                     /locus_tag="VYN80_001060"
     CDS             1876..2877
                     /locus_tag="VYN80_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1540984.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3028..3945
                     /locus_tag="VYN80_001061"
     CDS             3028..3945
                     /locus_tag="VYN80_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1540985.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3956..4846
                     /locus_tag="VYN80_001062"
     CDS             3956..4846
                     /locus_tag="VYN80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1540986.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4875..6491
                     /locus_tag="VYN80_001063"
     CDS             4875..6491
                     /locus_tag="VYN80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1540987.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6501..7820
                     /locus_tag="VYN80_001064"
     CDS             6501..7820
                     /locus_tag="VYN80_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1540988.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8030..8102)
                     /locus_tag="VYN80_001065"
     tRNA            complement(8030..8102)
                     /locus_tag="VYN80_001065"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8067..8069),aa:Lys,seq:ctt)
     gene            complement(8367..9638)
                     /locus_tag="VYN80_001066"
     CDS             complement(8367..9638)
                     /locus_tag="VYN80_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1540989.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            9908..10146
                     /locus_tag="VYN80_001067"
                     /pseudo
     CDS             9908..10146
                     /locus_tag="VYN80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10311..10490
                     /locus_tag="VYN80_001068"
     CDS             10311..10490
                     /locus_tag="VYN80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1540990.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10633..10806
                     /locus_tag="VYN80_001069"
     CDS             10633..10806
                     /locus_tag="VYN80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1540991.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            10843..11295
                     /locus_tag="VYN80_001070"
     CDS             10843..11295
                     /locus_tag="VYN80_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1540992.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11586..12056
                     /locus_tag="VYN80_001071"
     CDS             11586..12056
                     /locus_tag="VYN80_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1540993.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12075..13142
                     /locus_tag="VYN80_001072"
     CDS             12075..13142
                     /locus_tag="VYN80_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1540994.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            13144..13572
                     /locus_tag="VYN80_001073"
     CDS             13144..13572
                     /locus_tag="VYN80_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1540995.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13710..14660
                     /locus_tag="VYN80_001074"
     CDS             13710..14660
                     /locus_tag="VYN80_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1540996.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            14662..15405
                     /locus_tag="VYN80_001075"
     CDS             14662..15405
                     /locus_tag="VYN80_001075"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1540997.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQSVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15416..17212
                     /gene="pepF"
                     /locus_tag="VYN80_001076"
     CDS             15416..17212
                     /gene="pepF"
                     /locus_tag="VYN80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1540998.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            17459..18250
                     /locus_tag="VYN80_001077"
     CDS             17459..18250
                     /locus_tag="VYN80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1540999.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            18361..19029
                     /locus_tag="VYN80_001078"
     CDS             18361..19029
                     /locus_tag="VYN80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1541000.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         5880 a         3298 c         4170 g         5792 t
ORIGIN      
        1 atccgataat ttataaagag agaaagatat tcatgagaga tttattatct aaaaaaagtc
       61 ataggcaatt agaattatta gaattattat ttgaacataa acgttggttt catcgttctg
      121 aactagcaga gttattaaat tgtacagaac gtgcagtcaa agatgatcta tcccatgtta
      181 aatctgcttt tcctgacttg atttttcatt cttctactaa tggtatacgc attattaata
      241 ccgatgatag tgatattgaa atggtttacc atcatttctt taaacattca actcattttt
      301 cgattttaga attcatcttc tttaatgaag gttgtcaagc tgagagtatt tgtaaagaat
      361 tttatatcag ttcatcttcg ctctatcgta ttattagcca aatcaataaa gtgattaaaa
      421 ggcaatttca atttgaagtc agtctgaccc ctgttcaaat cattggaaat gagagagaca
      481 ttcgttactt ttttgcacaa tatttttcag aaaaatatta tttcctagaa tggccatttg
      541 aaaatttttc atcagagcca ctatctcaat tgttagaatt ggtttataag gaaacaagct
      601 ttccaatgaa tttgtcaacg catagaatgc taaagttgct cctagttacg aacctatata
      661 gaataaagtt tggtcatttt atggaagtag ataaagattc ttttaacgac caaagtttgg
      721 attttttaat gcaggcagaa ggaatagaag gtgttgctca gagttttgaa tcagaataca
      781 atatctcttt agatgaagaa gttgtttgcc aattatttgt gtcttacttt caaaaaatgt
      841 ttttcataga tgaaagtctc tttatgaaat gcgtaaaaaa ggatagttat gttgaaaaat
      901 cttaccatct attgagtgat tttattgatc agatttcagt caagtatcag attgaaatgg
      961 agaataagga taatctgatt tggcatctgc ataataccgc acatctgtat cgtcaagagt
     1021 tgtttactga gtttatttta tttgatcaaa aagggaatac aatcaggaac tttcaaaata
     1081 tttttccaaa atttgtttca gatataaaaa aagagctttc tcactattta gagactcttg
     1141 aggtttgttc tagttcaatg atggtaaatc atctatctta taccttcatc actcatacca
     1201 aacatttggt gattaatttg ctacaaaatc agcctaaact aaaggtttta gttatgagta
     1261 attttgatca gtatcatgca aaattcgttg cagagacact ttcttattac tgtagcaata
     1321 attttgaact ggaagtttgg actgaattag aattatcaaa ggaatcttta gaagattcac
     1381 cttatgatat cattatttct aattttatta ttcctcctat tgaaaataag agactcatct
     1441 attcaaataa tataaacacg gtctcactca tatacttgtt aaatgccatg atgtttattc
     1501 gattagatga gtaaccaaaa ataacaaaaa aacgctattt cgcgtttgaa ctgtacctca
     1561 aaagttagac agaaaaaatc taatttttgg ggtgtttttg ttaagaaaag accttcaatc
     1621 atttaagcaa aatgtacatc tagatttata gccctctaaa actcttgctt tttttgtaga
     1681 agatacgaaa aaagccataa aaccaaggct tttcctgttt tatgtagatg tttacacatg
     1741 aaaattgaag tattttcaaa agctaattgg agtgatttta ttcatgatgg gataaatgat
     1801 ctggccaaaa atgattgaga aatagttgct taaaaagcat ttgcatgata ctatatattg
     1861 aaaggaggtg ataatatggt agaacaaaga aaatcaatta ccatgaaaga tgttgcttta
     1921 gaagcaggag ttagtgttgg aactgtttca cgtgtaatta ataaagaaaa aggcattaaa
     1981 gaagtaactt tgaaaaaagt ggaacaagcg attaaaactt tgaattacat tccagattac
     2041 tatgctagag gaatgaaaaa aaatcgaaca gaaacgattg caatcattgt accaagtatc
     2101 tggcatccct tcttttcaga atttgctatg catgtggaaa atgaagtcta taagagaaat
     2161 aacaaattac tcttatgttc tataaatggt acaaatagag agcaagacta tctggagatg
     2221 ttgcgtcata ataaagttga tggagtggtt gccattacct atagtccaat tgaacattac
     2281 ttgacgtcag gaattccctt tgttagtatt gaccgcacat actcagatat tgccattcct
     2341 tgtgtttcat cagataatga tgcaggtgga agagaagcag ctaaacaatt aattagtaaa
     2401 ggatgtcagc atttagcctt tgtgggcgga cataatacaa ccattaatga aaccaaacgt
     2461 cgtagaattt catttgaaaa gtatgtccaa gagcataaaa tacctcttag tatttttgat
     2521 ttggatgaga cagttactga ctattatgga aagctagaga gtttcttgga agaaaattct
     2581 tctatagatg gaatttttac aatcaacgat tttacagctt tggatgtgat tgaaatacta
     2641 gaaaaaaatg gcaaacgtat tcctcatgat gtccaaatta ttggatacga tggaattaaa
     2701 atggctggag atagagatta tctactttca actattgaac aaccattgga agaaatggca
     2761 aaagaagcgg ttcgtatttt gtttgatata attgatggaa agactgttaa tttgcagaca
     2821 atcctaccag taaaatttgt tgaaggaaaa acaacaaaaa atgaaaataa gtcttgacag
     2881 aaagcgctat caatgataga atgaattcag ataaaaagat ttatttttaa aacaaaaatg
     2941 aaacgtttca aaaaaagaaa taaaaagaca gcgccaagcg ctatctttta tagaaaaaat
     3001 gaaacgtttc aaaaaaagga ggttgctatg aatagcaaag cgaagcaagt ttctctttgg
     3061 gaaagaatca agaaacaaaa actcttgtta ttgatgactg tccccggttt agttttaacc
     3121 tttatcttta aatacatccc tatgtatggg gttttaatcg catttaaaga ttacaatcct
     3181 ttaaaaggaa ttttagggag tgattggatt ggtttttctg agtttacaaa attcatatcc
     3241 tctcccaact ttggtatctt gttagccaac acattaaaat taagtatcta tggtttattg
     3301 cttggctttt taccaccaat cattcttgcg attatgctca atcaactctt gagtgaaaaa
     3361 gtcaaaaaac gaattcagct cattttatac gcaccaaact ttatctcagt cgttgttatt
     3421 gtcggtatga ttttcctctt cttttcagtg ggaggaccaa tcaacaattt tctttctatg
     3481 tttggaatga aggctgactt cttgacaaat ccagacttct ttagaccctt atacatcttt
     3541 agtggtatct ggcaaggaat gggctgggct tcaacgctct acacggcaac attggtaaat
     3601 gtagatccag ccttagtaga agcagctcga ctggatggag ccaatatctt ccaacgaatc
     3661 tggcacattg atattccagc tcttaagcct attatggtta tccaatttgt tttagctgca
     3721 ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga tgcagacatc gttaaatttg
     3781 ccaacttctg aaattatctc gacatatgtc tataaagttg gtcttgtatc aggagactat
     3841 tcttactcaa cagcggttgg tttgtttaat gcagtgatta acgtagtatt gcttgttgca
     3901 gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa tttaaggagg aaagtatgaa
     3961 aaattcgatt atggatacaa aatttgatag acgtatctta ctcttaaata aaatcattat
     4021 tgtctttatc gttttgatga ctttgcttcc tttactttat atcgtcgtag catcctttat
     4081 ggatcctaag gttctggtta gtagagggat tagctttaat ccagccgatt ggactgtaga
     4141 aggttaccag cgtgtattca gtgaccaatc tattctaaga ggttttatca attctctact
     4201 atactctttt ggatttgcag ctttaacagt cttgctatct gtgtttacag cttatcctct
     4261 ttctaagaaa gacttggttg gacgtcgttg gattaactac ttcttgattg taactatgtt
     4321 ctttggtggt ggtttagtcc caacttactt gctcgtaaaa gaattgggaa tgctcaatac
     4381 tccatgggct atcattgttc caggtgctgt caatgtttgg aatattattc ttgctagggc
     4441 ctatttccaa ggattgcctg aagaattagt tgaagctgct gtcattgatg gtgcaaatga
     4501 tttacagatt ttcttcaaaa tcatgcttcc tcttgcaaaa ccaattatgt ttgttctctt
     4561 cctttatgct tttgtaggac agtggaactc atactttgat gcaatgattt atatcaagga
     4621 tccaaacttg gaaccattgc aacttgtact tcgtaaaatt ctcattcaga gccaaccagg
     4681 tcaagacatg attggagcac aagcggctat gaatgaaatg aaacgtttag ctgaattgat
     4741 taaatacgca actattgtca tttccagctt gccattgatt gttatgtatc cattcttcca
     4801 aaaatacttt gataaaggaa ttatggctgg ttcacttaaa ggataaaaaa agaaaaaata
     4861 aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc agcagttttg ttgacagcta
     4921 gtttagcagt acttgcagcc tgtggctcaa aaaatacagc ttcaagtcca gattataagt
     4981 tggaaggtgt aacattcccg cttcaagaaa agaaaacatt gaagtttatg acagccagtt
     5041 caccgttatc tcctaaagac ccaaatgaaa agttaatttt gcaacgtttg gagaaggaaa
     5101 ctggcgttca tattgactgg accaactacc aatccgactt tgcagaaaaa cgtaacttgg
     5161 atatttctag tggtgattta ccagatgcta tccacaacga cggagcttca gatgtggact
     5221 tgatgaactg ggctaaaaaa ggtgttatta ttccagttga agatttgatt gataaataca
     5281 tgccaaatct taagaaaatt ttggatgaga aaccagagta caaggccttg atgacagcac
     5341 ctgatgggca catttactca ttcccatgga ttgaagagct tggagatggt aaagagtcta
     5401 ttcacagcgt caacgatatg gcttggatta acaaagattg gcttaagaaa cttggtcttg
     5461 aaatgccaaa aactactgat gatttgatta aagtcctaga agctttcaaa aacggggatc
     5521 caaatggaaa tggagaggct gatgaaattc cattttcatt tattagtggt aacggaaacg
     5581 aagattttaa attcctattt gctgcatttg gtatagggga taacgatgat catttagtag
     5641 taggaaatga tggcaaagtt gatttcacag cagataacga taactataaa gaaggtgtca
     5701 aatttatccg tcaattgcaa gaaaaaggct tgattgataa agaagctttc gaacatgatt
     5761 ggaatagtta cattgctaaa ggtcatgatc agaaatttgg tgtttacttt acatgggata
     5821 agaataatgt tactggaagt aacgaaagtt atgatgtttt accagtactt gctggaccaa
     5881 gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt tgcacgtgac aagatggtta
     5941 ttaccagtgt aaacaaaaac ctagaattga cagctaaatg gattgatgca caatacgctc
     6001 cactccaatc tgtgcaaaat aactggggaa cttacggaga tgacaaacaa caaaacatct
     6061 ttgaattgga tcaagcgtca aatagtctaa aacacttacc actaaacgga actgcaccag
     6121 cagaacttcg tcaaaagact gaagtaggag gaccactagc tatcctagat tcatactatg
     6181 gtaaagtaac aaccatgcct gatgatgcca aatggcgttt ggatcttatc aaagaatatt
     6241 atgttcctta catgagcaat gacaataact atccaagagt ctttatgaca caggaagatt
     6301 tggacaagat tgcccatatc gaagcagata tgaatgacta tatctaccgt aaacgtgctg
     6361 aatggattgt aaatggcaat attgatactg agtgggatga ttacaagaaa gaacttgaaa
     6421 aatacggact ttctgattac ctcgctatta aacaaaaata ctacgaccaa taccaagcaa
     6481 acaaaaacta gaggttgatt atgggagata agaaatacac agtagaaaaa gccaatcgtt
     6541 ttatagcaga aaataaacat ctcgttaata ctcaatataa gcctgaagaa catttttcag
     6601 ctgagattgg ttggatcaat gatccaaatg gatttgttta ttttcgtgga gaataccatc
     6661 tcttttatca attctatcca tatgatagtg tttggggacc tatgcactgg ggacatgcta
     6721 aaagtaagga cttggtgact tgggagcact tgccagtggc acttgctcct gatcaagatt
     6781 atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa ggatgatcgc ctctggctca
     6841 tgtacactgg gcatatcgaa gaaaaaaacg gtgtccgcca agtgcaaaat atggcatttt
     6901 cagacgacgg gattcacttt gaaaagattg aacaaaatcc agttgcaaca ggagcagact
     6961 tgccagatga gttgattgct gctgatttcc gtgatccaaa actctttgaa aaagatggac
     7021 gctattactc cgtagtagct gccaaacaca aggataatgt gggctgtatc gttctactag
     7081 ggtccgataa cctagtagaa tggcagttcg aatccatctt tttaaaaggg gtagaacacc
     7141 aaggctttat gtgggaatgc ccagattact tcgagttaga tgggaaagat tgccttatta
     7201 tgtcacccat gcgttatcag cgtgagggag actcatatca taacatcaac tcatcgcttt
     7261 tgttcacggg taaggtagag tggggagaaa aatgttttat cccagaatca gttcaagaaa
     7321 ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt ggacgatcaa aatcgtcgta
     7381 tcctgattgc ttggatgcag acatgggggc gtacccttcc aacccatgac caagaacaca
     7441 aatgggcatg tgccatgact ttacctagaa ttctaagatt ggaagatggc aaactaagac
     7501 aattccctgt taaaaaaggc caatatcaaa tccaaataga taaagattgt cattaccact
     7561 taggaaatga tatagattat cttgaatttg gttatgacag taatgcgcag caagtttaca
     7621 ttgatcgtag ccatcttatt caaaaaattc taggtgaaga agaacaggac actagtcgac
     7681 ggtatgtaga tattgaagct aaagaattgg aagttgttct agataaaaat tccatcgaga
     7741 tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta ttacttaacg gtgccagctg
     7801 agctatcacg aattgattaa aaattaagtt atttctccta aagaaaaagt tctctttcta
     7861 aaatagtgga aagaggactt tttgtgtttt gggtatataa gcttagttta tggtatttgt
     7921 aaaattggtg ttggattatg atttaagcta gttttctaaa gaatttgaaa aaaattttat
     7981 ttaagcaaaa aaaaaccttg gttccaagac ttttcctgtt gtatttagat gccccctaca
     8041 gggatcgaac ctgtgaccca cggattaaga gtccgctgct ctgccagctg agctaaggag
     8101 gcaaagaaaa aagctgtatt ggtgccgaaa cttcacggtt tgtattgaac ccgcgcaatt
     8161 aagcaggtgg gcaactcgct ctaactgaag ctgtttccgt gtgagacggc ctacatgctg
     8221 ttagaagact tttgtttccc taataataca aaaaatagtc ggtcaacact taagtgtgaa
     8281 gtcgtacacc acagcgtttc tatacttata tgataccact ttttcaaaaa aaatcaagag
     8341 gaaagtgcaa ttttttgaaa aggatttcag atttttcgca aatggtcgat agcttccttt
     8401 ctttgagcca aagcccgttc atatttacca gtatcttctg cttggaaata gtgatgatta
     8461 cgaatttttt ctggcagata gtcttgcttg acccaatttc caggatagtt gtgtggatag
     8521 agatagtctt gggcattccc cagttccttg cttccactgt agtgcccatc acgcaggtgt
     8581 cgcggaatag gcaagtgccc tgatgttttg aggtcagcaa gtgccttatc catagctaca
     8641 taggctgagt tggattttgg agaaagggcc aaatcaatca cgacattggc gatgagaatg
     8701 cgggcttctg ggaaaccaat cctttgtgca gcatccagag cagtcacggt atgaatctgg
     8761 gcctcaggat tggctaaacc gatatcttca taggcgataa cagtcaagcg acgagcgaga
     8821 ctaggcagat ccccagcctc aatcaagcgg gcagcatagt ggagactggc atccacatct
     8881 gagccacgga tagacttttg cagggcagat agaacatcat agtgaccgtc tccatccttg
     8941 tccatagtga tgtagcttct ctgaagacta ttttccatga tgtctaaggt gatgtggcga
     9001 atgccctcgt cattctcagg ggtagagaga acagccaagt ccagtgagtt aaaggcagag
     9061 cgaaggtctc cgtttgtaga ggttgcgatg aaatccagcg catcctcatc tagttctatt
     9121 ggaaaatcaa aaccacgttc agggttactt agagctatct gcagggcctc tttgacgtct
     9181 tggttagaca gaggttccaa ctcaaaaatt tgaacacggc tacgaatggc aggagtgacg
     9241 gaaaagaaag gattttcagt cgttgctcca atcatgatga ccagtccact ttccaagaga
     9301 ggcaagagga agtcttgctt ggtcttatct agtcgatgaa tttcgtctag caataggacg
     9361 agaccaccag aaaatttagc ttcttccgag atttcttgca gtctcttttt actatcaact
     9421 gtcgcattga aagttcgaaa ggcatacttg gtcgttccag cgatggcaga ggcaatactg
     9481 gttttgccga ttcccggagg gccatataga atcatggagg acaggcggtt ggcttccacc
     9541 atgcggcgga tgatttttcc aggtccgacc agatgctcct gaccgatgac ctggtcgatg
     9601 gttttagggc gcatgcgaag cgcgagattg tctggcatag cagtcctttc tagcgtggat
     9661 ttgagtagaa tgactcatct actatatctt actatgtcca ttctagccta ttagttgtga
     9721 tatttcaatg gttttgtaat aatcagggag tgagatagaa tcttttttac tatttaattt
     9781 aatgaggaga ggtatcagca tgagagattt gattttcctt aaagctcaaa ataggatagt
     9841 tgagcctaaa gaagtggaag tgctgacttg agaaataata ttgctgaatg tcattgtatg
     9901 aactataatg aaaagagagg tgataagcaa tggaaatgat ggaccaagtc aggaaatttt
     9961 gatttttaca aaacaaattc gtcattggat tcttagtgat caagttattt cagggaaaag
    10021 aaaacttttc ttccggaaga tactccaaaa gagattttag atttgtacga aaacattaaa
    10081 tctaaacttg attttgctta ccaagaagtt cattctaata atgggttgaa aaaatatgaa
    10141 aaatagaata attgatgttt ttgaagtagt aaatcgcctc cttgtaataa ctgttgaaaa
    10201 tcctgatttc gaagacttga gagttaatca tactcaatga aaatcaaaga gcaaactagg
    10261 aaactagccg caggctgtac ttgagtacgg taaggcgacg ttgacgtggt ttgaatttga
    10321 ttttcgaaga gtatcagttt gtaaaaattg gagataaaaa atatagagta cgcagtggtc
    10381 ctatgattca ttcaactccg cctcaatctg tcttagatcg agatactttt acaattgact
    10441 atacagatga tgaattgctc gataaagaag cagtttttac tactcattag aaaaataggt
    10501 atgaataaat tggtttccct tagaaccaca tctaggggct tttatgtata atagaataat
    10561 aagtatcact cctttacaat acgtgtaaca cgtgttataa tggtgttgtc aggaggtaaa
    10621 agcgctatac ctatgacaca aaaagagatg gttaaactcc ttacggccca tggttggata
    10681 aagactagag gcggtaaagg ctcccatatt aaaatcgaga agcaagggga aagacctatc
    10741 acaatccttc atggtgagct gaataagtac actgaaagag ggataggaaa gcaagctggg
    10801 ttgtaaaatt cagtccctgc tttttgatat ggaggtagtg aaatgttagt tacgtatcca
    10861 gctttatttt attatgacga cacagacgga acagaagcga cttattttgt ccatttccca
    10921 gattttgaat actcagctac acaaggagag gggatttctg aggctttggc tatggggtcg
    10981 gagtggctag ggataactgt tgcagatttg atagagagtg atggagagtt acctcagcca
    11041 tcagatatca atagcttgtc tttaattgat aatgatcctt ttaaagatga tgaagatttc
    11101 gtgtcaacct atgaccttga taaatctttt atttctatgg tatctgttga tgtatcagaa
    11161 tacctaggaa gtcaggaacc cattaaaaag actttgacca taccaaaatg ggcagataag
    11221 ttgggacgag aaatgggact taacttttct cagactttga cagacgctat tgcagataag
    11281 aaagttcaag cctaagatta agcgtggtat cgtggtaata gtcaataaaa aagcacgtcc
    11341 acttgtgcta gttactcgcc tattgatttt atagatttat tagccctttc gagggctttt
    11401 tatattgatt tttatgagaa atgaagaaat cagactttta agaaaatcac tgataccaag
    11461 ggtttaaatg aggtaatatg gtataattag gacataaaat aattttgtgg taagatggta
    11521 gtatctattt tagcatattt ccgagcaatc ggggcgatta aagagtcgca tagaaagagg
    11581 acaaaatggc aacatacgga tttttagata ttttagagga agagttggac aagaactttc
    11641 cctttgactt tgagattagt tgggacaagc gtaaccacgc ggtagaagtg agttttctat
    11701 tggaagcgca aaatgctgca ggtgtggaga tggtggatga agacggagag gtttcatcag
    11761 atgacattct ttttgaggaa gcggtccttt tctacaatcc tgccaagtca accgtcaatg
    11821 cagaagacta tttaacggtt atcccttatc tacctaaaaa aggattttct cgcgagtttt
    11881 tagcttattt tgcccttttc ctcaaagata ctgcggaggt tgggctagat accctcatgg
    11941 actttttgga agatccagaa gcagaggaat ttgttatgga atggaatcaa gaagtctttg
    12001 aagaaggaaa agttggcttg gaagagggag aattttatcc ttatccaaga tactaggagt
    12061 tggttggagg tgttatgaag aaaattggga cttatttggt ttatgtgcta gctgttgtct
    12121 ttattatgct agcttttgct tgtggaacaa tcgcatttgc agagttgggg tattccgcag
    12181 ttttagcctt tacttttggt tatgccttcg ctcttctaag catgtattta atctttattc
    12241 ttcatgagct gggtcatgct ttttgtggct acttgacagg ctatcggctg gtggcttttg
    12301 gattaggaca ttttattttg accaaaaagt caggcaggtt tcatcttagt agaacagcca
    12361 ttctgaaaaa tgttggtgct caatacattg gtttaaaaga ggatgaaagc gatcaaagaa
    12421 tcatcctgat gctttcagga ggcttgatgg ttcatctcgg cttgatatta ttggcgatag
    12481 tgtttggatt tttgacaaga agctggtatt ttgcagggac ttggattttt cttaatttat
    12541 ctttcttcct aaataacatt ttgccagtcg acatcaccga tggagcaaaa atttgggaat
    12601 tactacaaca ccctgaaaat accaaatacg cctacctggt attgagacat tctgctcaaa
    12661 ctttgctggc tcctcaagaa tatgatttga aagactttgt tcaagcagtt cctgaggatg
    12721 cacgagggag ttttgcggat ggtgtattag gtatgcaagg tgaagtttcc attttggaag
    12781 ggaaggaaga agtagctaaa cagcagtttc aagcattact tgagaggaca actactccga
    12841 tgatgcagac agttgcccaa ctatccctgc ttcatatcgc cttgttggaa ggagattttg
    12901 agcaagcgga gcaatatgcc agtattcgac aggtcaaatc gtttctgtct ctcaaactgt
    12961 ccaatcttca agtgatacaa gcctggtatc aattaaaggt gaagaaagat gtagatcgaa
    13021 ctcgtaaggc tctcaagatt gctagacaga aaatggatag tagtcgaatg ttgagggacg
    13081 agaaacgcta ttatgaaaac ttgttagcag agctggaaaa ggaattagcc gaaggagtct
    13141 aatatggaaa tagagatttc tgatttcaca ggttgcaaga ttgctttgtt ttgtggggat
    13201 aagcttttga ctatcttacg cgatgataag gcaagcattc cctgggccaa tatgtgggaa
    13261 ctgccaggtg gtggacgtga aggtgatgaa agtccttttg agtgtgtagc gcgtgaagtt
    13321 tatgaagaac tgggcattca tctgactgaa gattgtctgc tttggagtaa gatttaccca
    13381 agtatgcttt ttgcagacaa acagtccgtt tttctagttg gtcagttgac acagaaccag
    13441 tttgacagta tcgtatttgg agatgaagga cagggctatc agctgatgaa tgttgaggaa
    13501 tttcttagtt ccagtcaagt tgtgcctcag ttgcaagaga gattaaaaga ttatttaaaa
    13561 gtaagtgatt agaaaggatg gttcggttaa atttctaaac tgaacccgcc ctaaacacgg
    13621 tgccaaaaag ataaacttct cttagacaca aacgtcttca gagaatttcc tattttggtt
    13681 ttgtgtttta cgggcttggt atcttaataa tggaaacatg gcaagagtta aaagttacag
    13741 tgaagcgtga gggagaggag ttggtttcca atctcttgat tgagctagga gcgcaaggtg
    13801 ttgcgattga agacagcatg gactatgtgg gaaatatgga ccgctttggc gagattttcc
    13861 cagaggtgga gcagcaagaa gaaatcgtag tgacagccta ctaccctgac acggtggatg
    13921 tgacagtggt tgaggcggac ttgcaggctc gcttagcaga attgacggat tttatggatt
    13981 tgggagagtt aaaaataggt acgactgcct tggctgagga agactgggca gacaactgga
    14041 agaaatacta tgagccagct cgtatcactc atgatttgac catcgttccc tcttggacag
    14101 actatgaggc gactgcgggg gaaatgatta tcaagctgga tcctggcatg gcttttggta
    14161 ctggaaccca cccaactacc aagatgagcc tttttgcctt ggaacaggtt cttcgtggtg
    14221 gcgaaacggt gctagatgtg gggactggtt caggggttct ctctattgcc agctcgcttc
    14281 tgggggctaa agaaattttc gcctatgacc tggatgatgt ggcggttcgt gtggctcagg
    14341 aaaatattga gctcaaccct ggcatggaaa atatccatgt agcggctgga gatttgctta
    14401 agggtgtgga aattgaggca gatgtgattg tggctaatat cttggcggat atcctcattc
    14461 atctaataga cgatgcctat cgtttggtta aggacgaagg ctacctgatc atgagtggca
    14521 ttatcaagga taagtgggac atggtgcgcg agtcggctga atcagctgga tttttcctcg
    14581 aaactcacat ggttcaaggg gaatggaata cctgtgtctt taagaaaacc aaggatatct
    14641 ctggtgtgat tggaggctag catgcagcag tattttgtaa aaggcagtgc tatctctcct
    14701 gtcactatcg aggacaagga aaccagtaag catatgtttc aggttatgcg cttgaaagaa
    14761 gaggatgagg ttaccttagt ctttgatgat ggcatcaagc gcttggcgcg cgtgctggat
    14821 atggaaaatc gtcagtttga gttggtccaa gaattaaatg acaatgtgga actaccagtc
    14881 caagtgacca tcgcatctgg atttcccaag ggagacaagc tggagttcat cgctcaaaaa
    14941 gtaaccgaac tgggtgctag ccaaatctgg gcctttcctg cagactggtc agttgccaag
    15001 tgggatggca agaaattggg taaaaaagtt gaaaaactag aaaaaattgc ccttggagca
    15061 gccgagcaaa gcaagcgtaa tattgtacca agtattaaac ttttcgagaa aaaagcagat
    15121 tttctagctc agctggacca gtttgactct atcatagtag cctatgaaga atcagctaaa
    15181 gaaggagaag ccgctgcgct tctgcaatca gtcactggtc ttgaaacagg atccaaactg
    15241 ctctttatct ttggtccaga aggtggtctg tcacctgcag aaatcgaaag ttttgaagct
    15301 aaaggagccg ttttggcagg cctaggtcct cgtattttgc gagcagaaac agcaccgctt
    15361 tacgccttat cagcccttag tgttttagta gaattagaga aataagagga agaaaatgga
    15421 acaaaaacac cgttcagaat ttccagagaa ggaactctgg gacttaacag ccctatacca
    15481 agaccgtgag gatttcttgc gtgcaatcga gaaagctcgt gaagacatca accaatttag
    15541 ccgtgactac aagggtaatc ttcatacttt tgaggatttc gagaaggcct ttgcggaatt
    15601 ggaacagatc tacattcaga tgagccatat tggcaactat gcttttatgc ctcagacgac
    15661 ggactatagc aatgacgaat ttgccaatat tgcccaagct gggatggaat ttgaaacaga
    15721 tgccagcgta gccttgacct tctttgacga tgccttggtg gtagcagacg aggaagtctt
    15781 ggaccgtttg ggtgaattgc ctcacttgac ggcagctatt cgtcaggcca aaatcaaaaa
    15841 agcccactat ctaggggctg atgtggagaa ggccttgacc aatctcggtg aagttttcta
    15901 tagtccgcag gacatttata ctaagatgcg agctggggat tttgaaatgg ctgactttga
    15961 agcccatggc aagacctata aaaacagctt tgtcacctat gagaatttct accaaaatca
    16021 cgaggatgct gaggttcgtg agaaatcctt ccgttccttc tcagagggac ttcgtaagca
    16081 ccaaaatacg gctgcagcag cctatctggc tcaggtcaag tctgaaaaac tcttggctga
    16141 tatgaaggga tacgactctg tctttgacta tcttctagct gaacaagaag tggaccgtgt
    16201 catgtttgac cgccagattg acctcatcat gaaggacttt gcaccagtcg ctcagagata
    16261 cctcaagcat gttgccaagg taaatggtct tgaaaagatg acctttgcag actggaaatt
    16321 ggacttggac agcgccctga atcctgaagt gactattgac gatgcctatg atttggtcat
    16381 gaagtcggta gaacctttgg ggcaagaata ttgtcaggaa gttgctcgtt accaagaaga
    16441 gcgctgggtg gactttgctg ctaacagtgg caaggattcc ggtggttatg cggcggaccc
    16501 atatcgcgta cacccttatg tcctcatgag ctggacaggc cgtttgagcg atgtctatac
    16561 cttgattcat gaaatcgggc attctggtca attcatcttt tcagacaatc atcaaagtta
    16621 cttcaatgcc catatgtcga cctactatgt tgaagcaccg tcaaccttca atgaattgct
    16681 actcagtgat tacttagaga accaatctaa tgacccacgt caaaaacgct ttgctctggc
    16741 tcatcgcttg acagacacct acttccataa ctttatcacc cacctcttgg aagccgcctt
    16801 ccagcgtaag gtgtatacat tgattgaaga aggagaaacc ttcggagcaa gcaaactcaa
    16861 cagtattatg aaggaagttt tgacagattt ttggggcgat gccattgaaa tcgacgacga
    16921 tgcagctttg acttggatgc gccaagctca ctactatatg ggcttgtata gttacactta
    16981 ctctgctggt cttgtcatct cgacagctgg ttaccttcat cttaaaaact cagaaactgg
    17041 agctgaagac tggctcaatc tcctcaaatc aggtggtagc aagacaccac ttgagtcagc
    17101 catgattatc ggtgctgata tttcaacaga caaaccactc cgtgatacca tccaattctt
    17161 gtctgacaca gttgaccaga ttatcgccta cagtgctgag ttgggggagt aaggggaata
    17221 gaaaaatttt ttagaatgat gtttatgggc ttgttaagcg tcattctatt tgtcttagtt
    17281 gtagctattt tcaaaaatgt cgttgaaggt cgtgatgtta aatttgaact tactgtagaa
    17341 ctggtattga tagttattta cgtcatttta tatcagattg tatttaattg agaattcaat
    17401 attgagtcat ttaagactgc ctttttgaac agttgctaga aatgtagagg tattgaccat
    17461 gtatcaagat ttactaagaa aaatagcaga agaaaaacca aattataacc aggaggaaat
    17521 ccagtggttg cttgatcatt tgggagatcc ttctccagaa attcgcgatg accttgtttt
    17581 tacaagcttt gctataggga ttcaggaaga gctatttaca caggagcaat ttcatttcat
    17641 tgctgaaggg gtttcatctg atgtaggact agataaagag attgataagg taggtctgcc
    17701 aacacttgaa cgttctttta gggcacttgt ttatgcaaat ctcttgtatg cagatgccaa
    17761 ccagcaatcg gttttttatc agggattaca attagaaatt cgtaatgtct tttcaaatca
    17821 aggcttgcac tatctttcaa aagaaaagga tacaacaggt ttctcaagtc agtatggttg
    17881 ggttcatgct tttgcacatg gagccgattt actgacagag gtggtctgtc atccagactt
    17941 tcctataaac agaattcatg aagtatttga catacttggc caactattta aaagaatgtc
    18001 gattcgcttt acagatgatg aggattggcg tttagcaaga gtgatctatg aacctatttt
    18061 acaagggaag ttggagcaag aacaagtagc ttcttggata aaaactgttg attttccgat
    18121 agaagaaagg gaggattttt ccaaattttc caactttaga tcctgtctgg tggaagtcta
    18181 tgtccaactt gaccagagaa atagtttaca agatgagttg aaagaagcta tccagtcttt
    18241 tcaatactag agattagcta atagttctat gattttcaaa aaatttccaa tcaattttct
    18301 tgaaaccctt tcctactttt gatagactag tatctagttt ttgaaaaaag gagaaagaaa
    18361 atgaaaaaat ttgttgctga gttaatcggt acgttcatgc ttgtgttcgt tgggacagga
    18421 gctgttgttt ttggaaatgg ttttgatggc cttggtcacc ttggaatcgc ctttgccttt
    18481 ggtttggcaa tcgtggtggc agcctactca atcggaactg tttcaggtgc tcacttgaac
    18541 ccggctgttt cgattgctat gtttgtaaac aaacgtttgt catcttcaga acttgtaaac
    18601 tacatccttg gtcaggttgt tggagctttc atcgcttctg gcgctgtctt cttcctcttg
    18661 gctaactcag gtatgtcaac tgctagtctt ggtgaaaatg ccttggcaaa cggtgtcact
    18721 gtctttggtg gtttcttgtt tgaagtcatc gcaactttct tgtttgtatt ggttatcatg
    18781 actgtgactt cagaaagcaa gggcaatggc ccgattgctg gtttggtaat cggtttgtca
    18841 ttgatggcga tgattcttgt cggattgaag attactggac tttcagtaaa cccagctcgt
    18901 agcttggcac cagctgtctt ggtaggcggc gcagcccttc aacaagtttg gattttcatc
    18961 cttgcaccaa tcgctggtgg agttcttgca gcccttgttg caaaaaattt ccttggaaca
    19021 gaagaataat tgaaactcaa aaagccttgc tcctcatctt gaggaacagg gctttttcgt
    19081 atgatactct tcgaaaatct cttcaaacca cgtcagcttc accttgtcgt aggtatggtt
//
LOCUS       DAVLWA010000031        18345 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLWA010000031 DAVLWA010000000
VERSION     DAVLWA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.031"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(39..848)
                     /locus_tag="VYN80_001079"
     CDS             complement(39..848)
                     /locus_tag="VYN80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1541001.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKYDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(850..1653)
                     /locus_tag="VYN80_001080"
     CDS             complement(850..1653)
                     /locus_tag="VYN80_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1541002.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     INLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1663..3150)
                     /gene="tacF"
                     /locus_tag="VYN80_001081"
     CDS             complement(1663..3150)
                     /gene="tacF"
                     /locus_tag="VYN80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1541003.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRAFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3486..4193
                     /locus_tag="VYN80_001082"
     CDS             3486..4193
                     /locus_tag="VYN80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1541004.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIEKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4198..5220
                     /locus_tag="VYN80_001083"
     CDS             4198..5220
                     /locus_tag="VYN80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1541005.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5237..6106
                     /gene="licA"
                     /locus_tag="VYN80_001084"
     CDS             5237..6106
                     /gene="licA"
                     /locus_tag="VYN80_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1541006.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDATSI
                     KTKFDKIAPILQTIHASGKELRGEFAPFEEIKKYESLIEEKIPYANYEAVREEVFSLE
                     KRLADLGVYRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6096..6974
                     /locus_tag="VYN80_001085"
     CDS             6096..6974
                     /locus_tag="VYN80_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1541007.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            6986..7675
                     /locus_tag="VYN80_001086"
     CDS             6986..7675
                     /locus_tag="VYN80_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1541008.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     NAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7771..8619
                     /gene="dprA"
                     /locus_tag="VYN80_001087"
     CDS             7771..8619
                     /gene="dprA"
                     /locus_tag="VYN80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1541009.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9458..10501
                     /locus_tag="VYN80_001088"
     CDS             9458..10501
                     /locus_tag="VYN80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1541010.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10668..12755
                     /gene="topA"
                     /locus_tag="VYN80_001089"
     CDS             10668..12755
                     /gene="topA"
                     /locus_tag="VYN80_001089"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1541011.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            12861..13220
                     /locus_tag="VYN80_001090"
     CDS             12861..13220
                     /locus_tag="VYN80_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1541012.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYILMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13313..13945
                     /locus_tag="VYN80_001091"
     CDS             13313..13945
                     /locus_tag="VYN80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1541013.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            13989..14645
                     /locus_tag="VYN80_001092"
     CDS             13989..14645
                     /locus_tag="VYN80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1541014.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14664..15296
                     /locus_tag="VYN80_001093"
     CDS             14664..15296
                     /locus_tag="VYN80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1541015.1"
                     /translation="MNFQLAKYSLLKKFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            15324..15758
                     /locus_tag="VYN80_001094"
     CDS             15324..15758
                     /locus_tag="VYN80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1541016.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            15770..16807
                     /gene="leuB"
                     /locus_tag="VYN80_001095"
     CDS             15770..16807
                     /gene="leuB"
                     /locus_tag="VYN80_001095"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1541017.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKHAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            16804..17073
                     /locus_tag="VYN80_001096"
     CDS             16804..17073
                     /locus_tag="VYN80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1541018.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17077..17436
                     /gene="leuD"
                     /locus_tag="VYN80_001097"
     CDS             17077..17436
                     /gene="leuD"
                     /locus_tag="VYN80_001097"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1541019.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17598..18221
                     /locus_tag="VYN80_001098"
                     /pseudo
     CDS             17598..18221
                     /locus_tag="VYN80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         5744 a         3160 c         3887 g         5554 t
ORIGIN      
        1 ataacctcta tcttagtagt ttgtataatc cccctcaatc agtttttgcg ataagcttta
       61 atgctatgac tataccactc ttgcatttct tttgatggtg gtgtcatata atcgccatac
      121 atctgggtta aaaattggtc atattttttg ggaacaggca acatacggcc ctcaaactca
      181 gttaaaatca gttctttaaa ggtatcaact gggaagattt ctttcatccc ttccttaccg
      241 atcccaactc ctccttcata ttgaggagtg ttggttacag catttttgac tagttgatca
      301 attttcttgt aaaagtagcg aggattgaca aatcggagag cgtaccagct acataatcta
      361 agaaaatctt ttagtttgct atcaccgtga actgctcgtg attttttgat ataagctagt
      421 tgacgaagag ccacatactt atagctcttg tcgacaatgc tcaagtctgt aaatcgatca
      481 attgggaaga catcgatgaa aaggctggta tcatgacgct tgtacttaac atggtcttct
      541 ataacagtag aagtgtccaa aatcgatgcg aaattatgga agtaccaaga agatgtatcg
      601 taggaaagaa ccttgtagcg agggtgattt tcttcttcaa taatcttcag taaacgctca
      661 taatcctcac gataaaggga aatatcaata tcatcatccc aaggaatcat acctttgtgg
      721 cggatggctc caagcatggt tccataactg agaaaataag gaatatcata tttcttacaa
      781 gtctcatcaa tatagtccag cagggctagt tgaatttctt taatttcttt tttttctaaa
      841 tattgcatcc taatcctcca atttataagc gtgaaattca tgactgtaga agcgtttttc
      901 ttctggtggt agggtcatat aatctccata aaattgtgtc aaaatagtat caaatttttc
      961 aggtgcagga aggcttaaat tctcaaaggg taaattgatt gttttatcaa aggtaccact
     1021 tgggaagact tccttttcct taaattttga agggataaaa gccatatact gcccattttc
     1081 acgactatat ttttgaattt ctttctcgat tttatttgca aaataacgag gagaaaccgg
     1141 tcgaaggagc aaccaaaagg ctgttcgtat ccaatctttt aaaaggctat ccttatagac
     1201 aatattttta tgtttactga aagacagcag tttgaagctt tccagtttat aacaagtatc
     1261 aatgacctta ggatcatcaa agcgatctat agggaaaata tcgataaaga taccagactc
     1321 ataggttttt gtatttcgag tatcaataat tttagtcgta ctgtcggtta tcttgataaa
     1381 gttgttaaag tagttcttat cagtttctaa ggataggagc ttatacttgc ttttttcctt
     1441 ttgaaaaatg ttaataaatc gttggtagtc ttctctaggc atggacagat caatatcgtc
     1501 gtcccaaggg ataaagccct catgtcgaac cgccccaatc agagtaccgt agttaataat
     1561 atagttgata ttgtgctttt tacagagagt atcaatataa tccaaaattt ctaattcaat
     1621 ttgtttggca tcttcaacgg ttagttgttt cattttaaac tcctatgatt ttttaaattt
     1681 attttttaag gctaggacat ggtttaaaaa ttcatagaaa atgctatctt ttgtgaagac
     1741 aagtagacta atataagaga tagctgatag caagacaatc aaaccagtat taatcaaaaa
     1801 tggcaaatta atgaccatat ccacaggata catgaaatta atcaggaaat aaattgctac
     1861 aaaggaaagt gaaaagagag agtatcgaac agtatagcta aagatatgtc ccaagtggat
     1921 gagttgtttt ctatggatga aaatgatata gaaaacaagt agagaagcct ctgataacat
     1981 agttgtcagt aagtagtatt cgggagccac gatatggttg aaaaagagga gactattcaa
     2041 gcccaaattg agtagcccag caaagactgt atagactgtg atacgttttt catagccatt
     2101 ggtaaagaga atttgggaac caagaatggt atctaaggcc aggataatcg tacgaaaagc
     2161 gaagagagag gtcaagatgc cgcctccgat atatttttca ctaccgtaaa gtaggatggc
     2221 atttggtcct aaaaccatga gtccaaaact cagtggaatg ataaagaagt taaagattcg
     2281 actacctcta ttaaccagag aaacataggc ttctttgtct cctttcccca gatagtaact
     2341 gagacgaggc acactcactc caattgcacc tgttacaacc ccagctataa cggtcacaat
     2401 tcgctgagct atggtatagt aactaacgtt gacatcaatc cctgttttaa cgagaaagag
     2461 gcgatctaaa aaagtgaaga gcatattggc attggcaaag actaacatgg ctgtcagagg
     2521 gagaaagagt ggtttaaaat cacttaggtg aatcttaaca agtttgatgt ctcttttaat
     2581 ccaaaaataa ctaatcaggt agttaatcag cgtcgataaa ctcatcacaa gtgtatagac
     2641 aacaatatcg tgttcatttt taacaaataa gaaaatagag accagcatca ggatacggat
     2701 gaaggcagtt ttgtaaaaga gaaaactgta attttccaga gcttcattga cccattcgat
     2761 tgaaaaaatc tgggcaatga gttgaatccc cataacaagg tagacctttt tgacgattgg
     2821 attatcagta aagaagagag gataggctag gatatagaca gcagtggtca aaatcgtaca
     2881 agcgatgcac aaataaaaaa gactagaaaa ggctctgtta agatcttttt tgttatcctt
     2941 gacattactg atagccctta aaccgtagtt atagacacca taagttgcaa agggcaagaa
     3001 aaatgacaaa atagtgtcga ctgagttgaa gtaaccatag tcagttcggt ccaagacacg
     3061 cgcgacatag gttccagtta ggatgggaaa aataatattc aagacacgaa ttcccatgta
     3121 agatagagca tttaatttta tacttttcat tcaatttacc tcgtttttca ttatatcata
     3181 aagttagcta ataagaaatg aagggcagta agtcaagtaa tcactttgaa gtttcaaatc
     3241 ttaagtttta agttttcttt aaggaaagta tattattctg aaggactcta aaatttcgca
     3301 gccatttatt agtaattgct acagaattcc tagtcattac tagaaatgga ctagtttctt
     3361 tgaataatag aactgcataa ttctcctatt ctagaagggg aggaccagta tttcttttat
     3421 gataggacta gattgtggta taatagagag aataagtttt tttagtaaga caaaggagaa
     3481 aatagatgat ttatgcagga attcttgccg gtggaactgg cacacgcatg ggaatcagta
     3541 acttgccaaa acaattttta gagctaggtg atcgacctat tttgattcat acaattgaaa
     3601 aatttgtctt ggagccaagt attgaaaaaa ttgtagttgg ggttcatgga gactgggttt
     3661 ctcatgcaga agatcttgta gataaatatc ttcctcttta taaggaacgt atcatcatta
     3721 caaagggtgg tgctgaccgc aatacaagta ttgagaaaat cattgaagcc attgatgctt
     3781 atcgtccgct tactccagag gatatcgttg ttacccacga ttctgttcgt ccatttatta
     3841 cacttcgcat gattcaggac aatatccaac ttgcccaaaa tcatgacgca gtggacacag
     3901 tggtagaagc ggttgatact atcgttgaaa gtaccaatgg tcaatttatt acagatattc
     3961 caaatcgtgc tcacctttat caaggacaaa cacctcaaac attccgttgc aaggacttca
     4021 tggaccttta tggatctctt tctgatgaag agaaggaaat cttgacagat gcatgtaaaa
     4081 tctttgtgat caaaggaaaa gatgtggcct tggccaaagg cgaatactca aatctgaaga
     4141 ttacaaccgt aacagatttg aagattgcaa aaagtatgat tgagaaagac tagtaaaatg
     4201 attaatcaaa tttatcaact aactaagcct aagtttatca atgtcaaata tcaggaagag
     4261 gctattgacc aagagaatca tatccttatc cgtcccaact acatggctgt ctgtcatgcg
     4321 gatcagcgtt actatcaggg aaaacgtgat cccaagattt tgaataaaaa gcttccaatg
     4381 gcaatgattc acgagtcatg tggaaccgtc atttctgacc cgactggaac ctacgaggtt
     4441 ggtcaaaaag ttgtcatgat tcccaatcag tctcctatgc agagtgatga agaattctat
     4501 gaaaactaca tgacagggac ccatttcttg tctagtggat ttgatggctt tatgagagag
     4561 tttgtttctc tccctaaaga tcgtgtggtg gcttatgatg ctattgaaga tacggttgca
     4621 gccattacag agtttgtcag tgtgggcatg cacgctatga atcgtctatt gactcttgct
     4681 catagcaagc gggagcggat cgccgttatt ggagatggaa gtttagcttt tgtggttgcc
     4741 aatattatca actatacttt gccagaatca gagattgtgg ttattggtcg tcattgggaa
     4801 aagttggaac tcttctcatt tgccaaagaa tgctatatta cggataatat tcctgaagat
     4861 ttggcctttg accatgcttt tgaatgttgt ggtggtgatg gtactggacc agctattaat
     4921 gacttgattc gctacattcg tcctcaggga acgattctca tgatgggagt tagcgaatat
     4981 aaagtcaatc tcaatactcg cgatgcctta gaaaagggct tgattttggt tgggtcatct
     5041 cgttctggtc gcattgattt tgaaaatgct atccaaatga tggaagtcaa gaaatttgcc
     5101 aatcgtctta aaaatatcct ttatctagaa gaacctgtaa gagaaattaa agatattcat
     5161 cgtgtctttg caaccgattt aaacacagcc tttaaaacag tgtttaagtg ggaagtataa
     5221 gtactggagg ttaattgtgg agaaaatcat taaagaaaaa atttcttcct tacttagtca
     5281 agaagaggaa gtcctcagtg ttgaacaact gggtggaatg accaatcaaa actatttggc
     5341 caaaacaaca aataagcaat acattgttaa attctttggt aaagggacag aaaagcttat
     5401 caatcgacaa gatgaaaagt acaatcttga actactaaag gatttaggct tagatgtaaa
     5461 aaattatctt tttgatattg aagctggtat caaagtaaat gagtatatcg aatctgctat
     5521 tacgcttgat gcgacttcaa ttaagaccaa atttgataaa attgctccaa tattacaaac
     5581 tattcatgct tctggcaagg aattaagagg agaatttgct ccttttgaag aaatcaaaaa
     5641 atatgaatcc ttaattgaag agaaaatccc ttatgccaac tatgaagctg ttcgagaaga
     5701 agtcttctcc ttagagaaaa gactggctga cttaggtgtt tacagaaaat cttgtcatat
     5761 cgatttggtg cctgaaaact ttatcgaatc acctcaagga cgactttatt tgattgactg
     5821 ggaatattca tcaatgaatg atccaatgtg ggatttggct gccctctttt tagagtctga
     5881 attcacttcc caagaggaag aaactttctt atctcactat gagagtgacc aaacaccggt
     5941 ttctcatgaa aagattgcta tttataaaat tttacaagat actatttgga gtctatggac
     6001 tgtctataag gaagagcaag gtgcagattt tggtgactat ggtgtgaatc gttaccaaag
     6061 agctgttaaa ggtttggctt cttatggagg ttcagatgaa aagtaaaaac ggagttcctt
     6121 ttggccttct ctcaggtatt ttctggggct tgggtctaac ggttagtgct tatatctttt
     6181 cgatttttac agatttgtca ccctttgtgg tggctgcagc tcatgatttt ttgagcatct
     6241 ttatcttact agcttttctc ttggtaaaag aagggaaagt tcgcctctca attttcttaa
     6301 atattcgcaa tgtcagtgtt atcatcggag ccttgctagc aggccctatc ggtatgcagg
     6361 ccaatcttta tgcagttaag tatatcggaa gttctttagc ttcatctgta tcggctattt
     6421 accctgcgat ttcagttcta ttggctttct tctttttgaa gcacaagatt tcgaaaaata
     6481 ctgtatttgg gattgtcttg attattggag ggattattgc ccagacctat aaggttgaac
     6541 aggttaattc tttctacatt gggattcttt gtgctttggt ttgtgctatt gcatggggaa
     6601 gtgagagtgt tcttagctct tttgccatgg aaagtgaatt gagtgaaatc gaagccctct
     6661 taatccgtca agtaacttcg ttcttgtcct atcttgtgat tgtgctcttc tctcatcagt
     6721 catttactgc agtagccaat ggacaattgc taggtctcat gattgttttt gcagcctttg
     6781 atatgatttc ctacttggct tattatatcg ctatcaatcg cttgcaacca gccaaggcta
     6841 caggcttgaa cgtgagctat gtagtatgga cggtcttgtt tgcagttgtt ttcttgggtg
     6901 caccgctaga tatgctgacc attatgacgt cacttgtcgt cattgctgga gtttatatta
     6961 ttattaaaga ataaaggaga ttcgtgtgaa agccattatc ttagcagcgg gattgggaac
     7021 tcgcttgcgt cctatgactg aaaatacccc taaagccttg gttcaggtta atcaaaaacc
     7081 cttaattgag taccaaattg agtttctcaa agaaaaagga atcaatgaca tcatcatcat
     7141 tgttggttat cttaaagaac aattcgatta cttgaaagag aaatacggtg ttcgtctcgt
     7201 tttcaatgat aaatacgctg actacaataa cttttactct ctctatcttg taaaagaaga
     7261 attggccaac agctatgtta ttgatgcaga taactatctc tttaaaaata tgttccgcaa
     7321 tgatttgact cgttcgactt attttagtgt ttatcgtgaa gattgtacca acgaatggtt
     7381 cttggtctat ggagatgact acaaggttca agacattatt gttgatagca aggcaggtcg
     7441 catccttagt ggtgtatcct tctggaatgc tccaactgca gaaaagattg tcagctttat
     7501 cgataaggct tatgcaagtg gcgaatttgt tgatctctac tgggacaata tggttaagga
     7561 taatatcaaa gagctagatg tctatgttga agaattagaa ggcaatagca tttatgagat
     7621 cgatagtgtc caagactatc gtaaattaga agaaattctt aaaaacgaaa attaaagatt
     7681 ccaacatctg acaaaatagt cggatgtttt ttgatttttt acgaactttt acgaatagat
     7741 agatgagtag aaaaagaaat ggagttattt atgaaaatca caaactatga aatctataag
     7801 ttaaaaaaat caggtttgac caatcaacag attttgaaag tgctagaata cggtgaaaat
     7861 gttgatcagg agcttttgtt gggtgatatt gcagatatat caggttgccg taatccagcc
     7921 gtttttatgg aacgttattt tcagatagac gatgcgcatt tgtcgaaaga gtttcaaaaa
     7981 tttccatctt tctctatttt agatgactgt tatccttggg atttgagtga aatatatgat
     8041 gcgcctgtac ttttatttta caagggaaat cttgacctcc tgaaattccc gaaggtagcg
     8101 gtcgtgggca gtcgtgcttg tagcaaacag ggagctaaat cagttgaaaa agtcattcaa
     8161 ggcttggaaa atgaactggt tattgtcagt ggtctggcca agggcattga cacagcagct
     8221 catatggcag ctcttcagaa tggcggaaaa accattgcag tgattggaac aggactggat
     8281 gtgttttatc ctaaagccaa taaacgcttg caagactaca tcggcaatga ccatctggtt
     8341 ttaagtgaat atggacctgg cgaacaacct ctgaaatttc attttcctgc ccgtaatcgc
     8401 atcattgctg gactttgtcg tggtgtgatt gtagcagagg ctaagatgcg ttcaggtagt
     8461 ctcattacgt gtgagcgagc aatggaagaa ggacgcgatg tctttgctat tcctgctagc
     8521 attttagatg gactatcaga cggttgccat catttgattc aagaaggagc aaaattggtc
     8581 accagtgggc aagatgttct tgcggaattt gaattttaaa aatgacctaa gctagaattc
     8641 taagaaaaaa tcaattttaa gagaaaatga acccaacatt tccataataa aacgcatatt
     8701 agcaagtttt taacacttga taatatgcgt tttttctaag tggattagta gagtagagga
     8761 tttttctcat ataatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcaac
     8821 ctcaaaacag tattttgagc aacctgtggc tcgcttccta gtttgcgctt tgattttcat
     8881 tgagtataag ggaaagtata gtgaattgaa ataagatgtg aacaactcta tcaggaaagt
     8941 caaattaatt tatagaaata ttttagcagc caaggtgcac tgttatagat tcaatacaca
     9001 ataatttagt gtaattgaga aaggagaaat gattgtgatt gatgttggct aggttatgtt
     9061 caatgattcc taccgtctca aatcttgtca gtaaggaaaa ataaattctt caaaagtaga
     9121 gattacaagg cttgtttaag aaagaattca aagaccttga caaataaaaa taaaatggtt
     9181 attataagaa atggtctaaa atagatgatg atacttttcg aaaatctttt tacgtcagct
     9241 cagctttgcc ttgctgtgtt ttgagcaagc tacggttagc ttccgagttt gattttcatt
     9301 tactagaaat gaaactgatg agagatatca gtagacattt gagtcaggat attatggaaa
     9361 atgataaaaa gagctcgtga gattggcata tcagactact aaagtattga gtttgttagg
     9421 attttagcga ctagttagct gggaaaggaa gatatttgtg acaaataata aactgtattc
     9481 gttgatagaa tttagaaata agatatatga agaattagaa ctttccagaa gtgatttagc
     9541 gattttacta tgtgccatgc ttatcgcctc tatcggatta aatatggatt cgactcccgt
     9601 gattattgga gccatgttaa tctctccttt gatgacacct attctgggag tggggctctc
     9661 tctagctata tttgatttta aattgttaag aaaatctttt aaaatattag ctattcaaat
     9721 tcttgccagt ttaatagctt caacacttta tttttatctt tctcccattt cgtatgctag
     9781 ttcggaaatt gttgctagaa cctctccgac tatttgggat gttctcattg cttttgtagg
     9841 agggatagca ggtattattg gtgctaggaa aaaagagacc aataatattg ttcctggtgt
     9901 tgctattgca accgccttga tgcctcctct ttgtacagta ggttatgcta ttgcttctgc
     9961 taatctaaaa tttatcatag gctcctctta cctattcctc atcaattgta gctttattgt
    10021 cattgcgact tatataggtg ttaggttgat gatggttaag aaacattatt ttaaagataa
    10081 tgaagaagac tctaaaatgc gtaggatttt gcttctagtt gctgttttgc tgatgattcc
    10141 gagtttcatc tctgcaacga ctttagtgag agaaacgttg aaaaaagagt cccttaagaa
    10201 atttatatca gagcagtttc aggggtataa tattttgaaa aaaacctatt ctaaaaagac
    10261 tcatacccta aagctaacca tttcaggaaa ttatttgaca gaagaagaac tcgatatgat
    10321 ttcaagtaag agaggtgact atggtttaag tgatgtttct gttcaagttt cacaattgtc
    10381 tgattcagaa caacttagta aggaagagct ggtggagtat ttcttccagt atatcaagga
    10441 taaggaagca aaagaaaagg agaaagccaa taaatttgat acagagtctg aggagcaata
    10501 atttcttgag aatagctggt ttctctcgtg agtcttctat gtatatcaaa ggaagactga
    10561 ggttttaagt ataaaacttt tcttctatta tagtagctaa gtcttgacag ggttcaaaaa
    10621 atggtttaca ctttataaag tttattactt tgaaaaggtg tgatactgtg gctacggcaa
    10681 caaaaaagaa aaaatcaaca gttaaaaaaa atctagtcat cgtggagtcg cctgctaagg
    10741 ccaagacgat tgaaaaatat ctaggcagaa actacaaggt tttagccagt gtcgggcata
    10801 tccgtgattt gaagaaatcc agtatgtccg tcgatattga aaataattat gaaccgcaat
    10861 atattaatat ccgaggaaaa ggccctctta tcaatgactt gaaaaaagaa gctaaaaaag
    10921 ctaataaagt ttttctcgcg agtgacccgg accgtgaagg agaagcgatt tcttggcatt
    10981 tggcccatat tctcaacttg gatgaaaatg atgccaaccg tgtggtcttc aatgaaatca
    11041 ccaaggatgc agtcaaaaat gcttttaaag aacctcgtaa gatcgatatg gacttggtcg
    11101 atgcccaaca agctcgtcgg atcttggatc gcttggtagg gtattcgatt tcgcctattt
    11161 tgtggaagaa ggtcaagaag ggcttgtcag caggtcgcgt tcagtccatt gcccttaaac
    11221 tcatcattga ccgtgaaaat gaaatcaatg ccttccagcc agaagaatac tggacagttg
    11281 atgctgtctt taaaaaggga accaaacaat ttcatgcttc cttctatgga gtagatggta
    11341 aaaagatgaa actgaccagc aataacgaag tcaaggaagt cttgtctcgt ctgacgagta
    11401 aagacttttc agtagatcag gtggataaaa aagagcgcaa gcgcaatgct cctttaccct
    11461 ataccacttc atctatgcag atggatgctg ccaataaaat caatttccgt actcgaaaaa
    11521 ccatgatggt tgcccaacag ctctatgaag gaattaatat tggttctggt gttcaaggtt
    11581 tgattaccta tatgcgtacc gattcgactc gtatcagtcc tgtagcgcaa aatgaggcgg
    11641 caagcttcat tacggatcgt tttggtagca agtattctaa gcacggtagc aaggtcaaaa
    11701 acgcatcagg tgctcaggat gcccatgagg ctattcgtcc gtcaagtgtc tttaatacac
    11761 cagaaagcat cgctaagtat ctggacaagg atcagctcaa gctatatacc cttatctgga
    11821 atcgttttgt ggctagccag atgacagcgg ccgtttttga taccatggct gttaaattgt
    11881 ctcaaaatgg agttcaattt gctgccaatg gtagtcaggt taagtttgat ggttatcttg
    11941 ccatttataa tgattctgac aagaataaga tgttaccgga catggttgtt ggagatgtgg
    12001 tcaaacaggt caatagcaaa ccagagcaac atttcaccca accgcctgcc cgttattctg
    12061 aagcaacact gattaaaacc ttagaggaaa atggggttgg acgtccatca acctatgcgc
    12121 caaccattga aaccattcag aaacgttatt atgttcgcct ggcagccaaa cgttttgaac
    12181 cgacagagtt gggagaaatt gtcaataagc tcatcgttga atatttccca gatatcgtaa
    12241 acgtgacctt cacagctgaa atggaaggta aactggatga tgtcgaagtt ggaaaagagc
    12301 agtggcgacg ggtcattgat gccttttaca aaccattctc taaagaagtt gccaaggctg
    12361 aagaagaaat ggaaaaaatc cagattaagg atgaaccagc tggatttgac tgtgaagtgt
    12421 gtggcagtcc aatggtcatt aaacttggtc gttttggtaa attctacgct tgtagcaatt
    12481 tcccagattg ccgtcatacc caagcaatcg tgaaagagat tggtgttgag tgtccaagct
    12541 gtcatcaggg acaaattatt gagcgaaaaa ccaagcgtaa tcgcctcttc tatggttgca
    12601 atcgctatcc agaatgtgaa tttacctctt gggacaagcc tgttggtcgt gactgtccaa
    12661 aatgtggcaa cttcctcatg gagaaaaaag tccgtggtgg tggcaagcag gttgtttgta
    12721 gcaaaggcga ctacgaggaa gaaaagatta aataagagga ggggcccgaa agtaaatttc
    12781 aggctctttt tggttagaac ttgacaaaat acatcatttt atgcgaaact agaagaagat
    12841 tattttaact aggagaagtt atgcgtctta tctatctgat aattggtttt ttatcactgg
    12901 ccttggctat tgttggggtt gttttaccct tgttgcctac aacacctttc cttttgttgt
    12961 ctattgcttg tttctccaga agttccaagc gattcgaaga ctggctttat cataccaagc
    13021 tctatcaaac atatgtagct gattttcgcg agactaagtc tattacgcgt gaacgaaaga
    13081 aaaaaatcat cgtatccatt tacattttga tggggatttc tatttatttt gcccctctct
    13141 taccagtcaa aatcggtctg ggtgctttga ccatctttat cacttattat ctcttcaagg
    13201 tcattccaga caaagaatag ttaaaacagt agttatttac cttgataaaa ttgaaagcat
    13261 attcataaca atatgatata ataaatttga agtaatattc aaggagaatc aaatgattta
    13321 cgaattttgt gctgaaaatg tgactttact tgaaaaagcg atgcaggctg gagctcgtcg
    13381 gattgaactc tgtgataatc tagcagttgg tgggacaaca cctagctatg gagtgactaa
    13441 ggcagcggtt gaactggcag ctaactacga tacaaccatc atgaccatga ttcggccacg
    13501 tggtggtgac tttgtctata atgacctaga aattgctatc atgctagaag acatttgttt
    13561 gactgctcag gctggaagtc aaggggttgt atttggagct ttaactgctg ataaaaagtt
    13621 ggataagcct aatctggaaa agttaattgc tgcatcaaaa ggaatggaaa ttgtctttca
    13681 catggccttt gatgaactaa gtgatgaaga tcaaccggaa gctattgact ggctcagtca
    13741 agccggtgtc actcgtatcc taactcgtgc tggtgtgtct ggcgactcct tagaaaaacg
    13801 ttttgttcac tatcacagaa ttttggagta cgctaaaggt aaaattgaaa ttctaccagg
    13861 tggggggatt gaccttgaca accgtcaaac ctttattgac caggtggggg taacacaatt
    13921 gcatggtact aaggttgttt tttaaaaaat agaaaggaac tgctagcctt gggtagcagt
    13981 tttcacttat gtttgaaatt tttaaatcct atcaatttaa tcaagaaaag gctcatgatt
    14041 atggttttat agaaaatagc gaagtctgga catatagttg ccagattttg caaggtgact
    14101 ttgtcatgac tgtgtccatc actgctgata atgtgaactt tcaagtcttt gaccaagaga
    14161 ctggtgacct ctatcctcac gttcatatgg aaagcatgag gggaagtttt gtcggaaatg
    14221 tccgtgaggc ttgtctggag attctttacc agattcggaa ggcttgtttt gatgtgcaag
    14281 attttatctg tcatcagact aagcgtatca tgactcaagt tcaggaaaag tatggaaacc
    14341 agttggagta tctgtgggaa aaatcgcctg atacagctgt attgcgccat gaaggcaatc
    14401 aaaagtggta tgccgtcttg atgaaaatct cttggaataa gctggaaaag ggcagagaag
    14461 gacaagtgga agcagtcaac ctcaagcatg accaagtagc taatttgctt tcacaaaagg
    14521 ggatttatcc agccttccat atgagcaagc gctactggat tagtgtgtcc cttgatgata
    14581 ctttatcaga tgaagaagta ctggaattga tagaaaaaag ttggaactta acctctaaaa
    14641 aatgaaatat tttaataatt ttcatgaact ttcaattagc taaatattct ttactgaaga
    14701 aatttttaga aaatatagga tttaccacac tagaggaata tggtgccatc ttcaaatacc
    14761 tgattgagaa tgtcaagacg gatcgtcaga tcatctgttc gcctcactgt catgatgacc
    14821 tcggaatggc agtggcaaat agccttgctg ctgtcaagaa tggtgcagga cgtgttgaag
    14881 ggactatcaa tggtattagg gagcgagctg aaaatgctgc tttggaagaa attgcagtgg
    14941 ctctcaatat tcgccaagat tactaccaag tagaaaccag tattgtccta aatgagacca
    15001 tcaatacgtc agaaatggtt tctcgcttct ctggtattcc agttcctaaa aacaaagccg
    15061 tcgttggtgg caataccttc tcccacgaat ctggtattca ccaagatgga gtccttaaaa
    15121 atcctctcac ttatgagatc atcacacctg aattggttgg tgttaagatt ccgcttggaa
    15181 aattatctgg tcgccatgct tttgttgaga aactgagaga attggcccta gattttacag
    15241 aagaggatat caaaccactc tttgctaagt tcaaggcact ggccgataag aagtaagaaa
    15301 tcacagatgc agatcctcga gctttggtag ctggaaccat ggttgaaaat ccagaaggct
    15361 tccactttga tgatttacaa cttcaaactc atgcagataa tgacattgaa gcgctcgtta
    15421 gcctagccaa tatggatggt gagaaagtcg aatttaatgc gacagggcaa ggttccgttg
    15481 aagcaatctt taacgctatc gataagttct ttaaccaatc tgttcgtttg gtgtcctaca
    15541 ctatcaatgc ggtaacagat ggaatcgatg cccaggatcg ggttttggtc actgttgaaa
    15601 acagagatac agaaaccatc tttaatgcag cagggcttga ttttgatgtg ttgaaggctt
    15661 ctgctattgc ctatataaac gctaatacct ttgttcaaaa agagaatgca ggtgagatgg
    15721 gacgcagtgt ttcttaccac gatatgccta gtgtgtaaag gagaaggcta tggcaaagaa
    15781 aatagtagct ctagtaggag acggaattgg cccagaaatc atggaggctg gtttagaagt
    15841 tctggaggct ctagctgaaa aaacaggttt tgactatgag attgacagac gaccgttcgg
    15901 aggtgcagat attgatgcag caggacctcc cttacctgat gaaaccctta aggcaagtag
    15961 ggaagcagat gctatcctac tagcagctat cggtagtcct cagtatgatg gagcagcggt
    16021 tcgccctgaa caaggcctga tggctctccg taaggaactc aatctttacg ctaatattcg
    16081 tcctgtaaaa atctttgaca gtctcaagta tttgtcacca ctcaaaccgg aacgaatttc
    16141 tggtgtagac tttgtcgtgg tgcgtgaatt gacaggcgag atttactttg gagatcatat
    16201 tcttgaagag cgcaaagcgc gtgatatcaa cgactatagc tatgaggaag tggagcggat
    16261 tattcgcaaa gcctttgaaa ttgcaagaaa tcgcagaaaa atcgttacta gtatcgataa
    16321 gcaaaatgtt ctagcgacct caaaactctg gcggaaagta gctgaggaag tcgcacagga
    16381 tttcccagat gtaaccttgg aacaccagct ggtagactca gctgctatgc ttatgattac
    16441 caatcctgct aagtttgatg ttattgtaac ggagaatctt tttggagata ttttatctga
    16501 tgaatcaagc gtcttatctg gtacacttga ggttatgcca tcagccagtc attctgaaaa
    16561 tggaccaagt ctctatgaac ctattcacgg ttcagcacct gatattgcag gtcaaggaat
    16621 tgccaatcct acttccatga ttttatcagt tgccatgatg ttgagagata gtttcggacg
    16681 ttatgaggat gcagagcgta tcaaacatgc tgttgagaca agtctggcgg caggaatttt
    16741 aacgagagat ataggaggtc aggcttcaac aaaggaaatg acggaagcta ttattgcaag
    16801 gttatgaagt tagacgaaaa aattactcta gtccttttga tttggaatgt catcattttc
    16861 ttgatttatg gcattgacaa attcaaggca agaagaagaa cttggcgcat ccaagagaaa
    16921 atcttactca ttttagccct tacttgtggt ggttttgggg cctggttagc tggaatcact
    16981 tttcaccaca agactcgaaa atggtacttt aaaacagttt ggtttcttgg gatggtgacc
    17041 acactagtag ccttatattt tatttggagg taatggatgg cagggtcttc gagggaacac
    17101 gctgcttggg ttctagcgga ctatggtttt aaggtcgtga ttgcaggatc tttcggtgac
    17161 attcattaca ataatgaact caataatggc ctgttgccaa tcgttcagcc tagagaggtt
    17221 agagagaaac tagcccagct aaaaccaacc gaccaggtaa ctgtggactt ggaacaacaa
    17281 aaaatcatct caccagttga agaattcacc ttcgagatag atagcgagtg gaaacataaa
    17341 ctcctaaata gtttggatga tatcggtatt accttgcagt atgaagagtt gattgctgct
    17401 tatgaaaaac aacgaccagc ctactggcag gattagaaaa aatagaaaag gaaatatagt
    17461 aaactgaaat aagatgtaaa caaatgaatt agagcttaac atccatttcc agcaattttt
    17521 tagaaactac agtggactat tctggattca acacattata aaattatgac aaaacacatt
    17581 cacaagaagg ctacgacatt ttaaaaggtg agggcggatg tatcgtttgc cctactaaag
    17641 ttggttacat tatcatgacc agcaacaagg caggacttga gcgtaagttc gcagccaaag
    17701 aacgtaagcg taacaaacca ggtgttgttc tctgcggtag catggatgaa cttcgcgctt
    17761 tagcgcaact caacccagaa attgaagcat tctactaaaa acattggatg aagatattct
    17821 tcttggttgt atccttcctt ggaaaccaga agcctttgaa aaactcaaag catacgggaa
    17881 tggccgtgaa gaacttatga ctgatgtacg tggtactagc tgttttgtta tcaagtttgg
    17941 aaaagcaggt gaacaattgg ctgccaagct ttgggaagaa ggtaaaatgg tctacgcctc
    18001 atatgcttca atgacaaaac gattgaaact cgctatgaga gcaaggtgta atggtgtcta
    18061 tggtcgataa ggacggcaaa ctcatcccag aacaaggagg agcacgttca acttcaccag
    18121 caccagttgt gatccgtaaa gggcttgaca ttgataaaat catgatgcac ctgtcagata
    18181 cttttaactc atgggactac cgtcaggttg agtattatta agataaaaaa agaagtctag
    18241 tgttatgaga tattaaagct cctaacactg gactttttgt ttagaatttc ttttcttttt
    18301 ctataggata tggtattcta tgtagaaaat atatgttaat aagta
//
LOCUS       DAVLWA010000032        18068 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLWA010000032 DAVLWA010000000
VERSION     DAVLWA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.032"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(49..996)
                     /gene="arcC"
                     /locus_tag="VYN80_001099"
     CDS             complement(49..996)
                     /gene="arcC"
                     /locus_tag="VYN80_001099"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1541020.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(1161..2177)
                     /gene="argF"
                     /locus_tag="VYN80_001100"
     CDS             complement(1161..2177)
                     /gene="argF"
                     /locus_tag="VYN80_001100"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1541021.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(2235..3464)
                     /gene="arcA"
                     /locus_tag="VYN80_001101"
     CDS             complement(2235..3464)
                     /gene="arcA"
                     /locus_tag="VYN80_001101"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1541022.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPVYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            4219..5898
                     /locus_tag="VYN80_001102"
     CDS             4219..5898
                     /locus_tag="VYN80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1541023.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTDAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGVEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGFPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLETKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(5938..8022)
                     /locus_tag="VYN80_001103"
     CDS             complement(5938..8022)
                     /locus_tag="VYN80_001103"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1541024.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSLYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            8193..9473
                     /locus_tag="VYN80_001104"
     CDS             8193..9473
                     /locus_tag="VYN80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1541025.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSPEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            9564..12209
                     /locus_tag="VYN80_001105"
     CDS             9564..12209
                     /locus_tag="VYN80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1541026.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVCTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEVTIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            12293..13162
                     /locus_tag="VYN80_001106"
     CDS             12293..13162
                     /locus_tag="VYN80_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1541027.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            13156..15036
                     /locus_tag="VYN80_001107"
     CDS             13156..15036
                     /locus_tag="VYN80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1541028.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            15327..15689
                     /locus_tag="VYN80_001108"
     CDS             15327..15689
                     /locus_tag="VYN80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541029.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNIEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(15741..16590)
                     /locus_tag="VYN80_001109"
                     /pseudo
     CDS             complement(15741..16590)
                     /locus_tag="VYN80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(16535..16717)
                     /locus_tag="VYN80_001110"
     CDS             complement(16535..16717)
                     /locus_tag="VYN80_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1541030.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            17052..>18068
                     /gene="pcpA"
                     /locus_tag="VYN80_001111"
     CDS             17052..>18068
                     /gene="pcpA"
                     /locus_tag="VYN80_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1541031.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGN"
BASE COUNT         5210 a         3590 c         4023 g         5245 t
ORIGIN      
        1 ttgatataaa gatactagaa atagaataca tcttattagt aaaacaactt atcctttttc
       61 aataattgtt ccgctttcag attcaatcaa ggcgcctaga ttttcaaggg aagtaataac
      121 tgcttttcct tctggacgac cattgacaaa agcgatagct gcttctactt ttggaagcat
      181 gctacctggt gcaaactgat cttgtttgat atattcttcc agctgggcaa cattcacatg
      241 ttccaatttt tcctggtttg gcttgttgta gttaacaaat acataatcta cacctgtcaa
      301 aacgatgaag aggtctgcat caactaattc tgccaaacgt tgggaagcga agtctttatc
      361 aataaccgct tcgacaccag tcaaatgtcc attgttttct ttgacgacgg gaataccgcc
      421 accacctgca gctacgacga cttgaccatt atttaaaaga gtacggatgg tttcaatttc
      481 tttgatgtca acaggttttg gtgaggcaac gaccttacgc cagccacggc cagcatcttc
      541 cttgaaagtc gctccgctct tttcggcttc tgcttttgct tcttcttctg aatagaaagg
      601 accgattggt ttactcaagt taacaaaagc cggatcattt ttatctacga caacttgcgt
      661 tacaacagag gcaacatttt tttcgatgcc ttcatccaag agagcatttt gcaaagcatt
      721 tttcaaccag aaaccgatgc taccttctgt catagcgaca agtgagtcga gtgggaaggc
      781 agggttcttt tcagagtctg atgccaaatg ttggagcaag agattcccaa cttgaggtcc
      841 attaccgtga gtgataatca aatcatctcc atttttaatc aattttacaa gatgcttagc
      901 tgtttcaact aaagcttctt gttgagcctt tgctgatggg tcagaagaaa gaatcgcatt
      961 tcctcccaaa gctactacaa ttttacgatt tgccataaat tctcctttat cacactcaat
     1021 agaatgcgtt tagatttcaa tttaatgatt tttcacatat tttataagaa ataatagatt
     1081 accattacat aaaagaggac cggactaaag ctattagtcg cagccctcat agctgttggt
     1141 agacggttta ttatctaaaa ttatacttta ggaatataaa ggttaccaag tgtagcagcc
     1201 ataacagctt tgatagtgtg catacggttt tctgcttgat cgaagtggcg agcgtacttg
     1261 ctgcggaaga cttcgtctgt tacttccatt tcttctacac caaatttttc agcaacgtct
     1321 ttaccataaa cagtgtgagt atcgtggaat gctggcaagc agtgtaggaa gatcaagttt
     1381 tcattgcctg cttttttaac taagtccata ttgacttggt aaggtttaag aagagctaca
     1441 cgttctgcga atttgtcttc ttcacccatt gatacccaaa cgtctgtgta aagaacgtct
     1501 gcatctttaa ctgcttcatc agcatcttca gtgatgagaa catgtgcgcc actttcttta
     1561 gcaaatcctt ctgccaattc aacgatttct ttttctggga agagttcttt tggtgagaag
     1621 atgtgaacat tgacaccaag gatagcacct gttacgagca agctgttggc aacgttgtta
     1681 cgtccatcac cacagtatac caatgtcaag ccttccaagc gaccgaagtt ttcttgaaca
     1741 gtcaagtagt cagcgagcat ttgagttggg tgccattcgt cagttagacc gttccatact
     1801 ggaacgcctg agaattctgc caattcttca accatacgtt ggctgaatcc gcggaattca
     1861 atcccgtcaa acatacgtcc caatacttta gcagtatctt cagtagattc ttttttaccc
     1921 aactgaatat catttgctcc gaggtattct gggtgagcac caaggtcgat agccgcagtt
     1981 gtaaaggctg cacgagtacg agtagatgtt ttttcaaata ggagagcgat attcttgcca
     2041 gcaaggtagt ggtgttgaat attgcgtttt ttcaaatctt tcaagtgagc tgaaagacca
     2101 ataaggtatt ctaactctgc acgggtaaag tctttttctg ctaagaagct gcgtccttgg
     2161 aatactgaat ttgtcatttt attatttcct ctttctattt tttacatttt ctattgacga
     2221 atatcgaaca gcgattacac ttcttcacgt tcaaatggca tagacataca acgaggtcca
     2281 ccacggcccc gaaccaattc acttccgcga atcttaatca agcgaagccc gtattcttcc
     2341 aaaatcttat tggtcacggt attgcggtca taaacaacta ccacaccagg tgcgatggtc
     2401 aaagtgttag aaccgtcgtt ccattgttca cgcgcagctg ctacgatatt gccaccaccg
     2461 caacgaatca aatgaacttt ttctacacca aggttttgag caagaagttc agctaagtca
     2521 cctttctctt caacgatttt aagtttttcg ttttcgtaag taactgagta aacgtgaagg
     2581 tcgccttcga tttctgggtg aatagtgaac ttgtcatagt ctaccatagt gaagacagta
     2641 tccaagtgca tgaatttacg gttgttagca aattcaaagg ccaaaacttt cttgaagcca
     2701 acatttttct tgaagatgtt gaccaaaagt ttttcgatag aagctgcgtc tgtacgttga
     2761 gagataccta ctgcaaggac gtctttagaa agaactagct cgtctccacc ttcgatacgc
     2821 gtatcttctt cacggttgta gaccaaatcc acttttccgc catagactgg gtggtatttg
     2881 aagatatact taccgtagag tgtttcacgg ttacgagtgt ctgcaaacat gtggttaagc
     2941 gatacggcgt ttccaattgt tgcaaatggg tcgcgagtga aatagaggtt tggcatcggg
     3001 tcaattgcaa atggataatc tgattcaact aagtcagtta gatctttagc ttcgtcagga
     3061 atttctggca attcaacttt ttgaatccca gccattgttt tttcaaccaa ttcttggttg
     3121 tccttgatgc cgtgaagcaa ttcacgaata gcaaccttgg tttgacgatc acggatgttg
     3181 gcttcgtcta agtattcctc gataaattga tcgcggattt ctggagaggt caatgattca
     3241 gcagcgagtt gttctaggta tagaacctca attccttcat cgcgaagagc ttgggcaaat
     3301 gcatcatgtt ctttttgagc atcttccaag aaaggaatat catcaaaaag aagcctttca
     3361 agatagtccg gcaacaagtt ttctaactcc ttgcctggac ggtgcaacat aacttttttc
     3421 agtttcccaa tttctgagaa gacctgaatt ggatgtgaag acatgtattt tcctccttaa
     3481 aaattttgac ttggcaccat tttgggtacc gcttacatgt atgttataac gcaattccta
     3541 ggtaatgtca atggctataa tttctcggaa aataatataa attatgatgt ttagcacaat
     3601 tttatgttat tatacataac ataggataga atacatagat acaaatgcat aaacttctat
     3661 aaaaaggata taaaattttt ttaaaaaatt tttcaaaaaa tatacaaatg accaaaaagt
     3721 gtccaattat catctggtta tcacttggat ctgtataaag agaaatgttt gtgtgaaagg
     3781 ataaaaagat gcaacattct tttgaagata gattttggag aagcatttct tgttcaattg
     3841 tacaaattat agaatctttt ctgagtattt aggaagagat aattatctta ttgaccctct
     3901 ttttattttt tgttatagtc gacgaggtga tagtttagaa taatcactta ttcacaatct
     3961 gtggaaaagt ctggatagtg aatggtcttc acacaatgac ctgaaagaag cctgagaata
     4021 attatggaga gtagcattct gagaggtgtt agcagaacca tatgacagag ctgtttgaag
     4081 agggaatatt gaggagaaaa atcctgagcc taccagttgg agttggaaag agctggcggt
     4141 tagatcattt tttattatcc acaacctgtg gataaccttg tgaacaagag aagttgctaa
     4201 agaaggagat atataacgat gaagaaaatc aaaccgcatg gaccgttacc aagtcagact
     4261 cagctagctt atctgggaga tgaactagca gcttttatcc acttcggtcc taataccttt
     4321 tatgaccaag aatgggggac tggacaggag gatcctgagc gctttaaccc gagtcagttg
     4381 gatgcgcgtg agtgggttcg tgtgctcaag gaaacgggct tcaaaaagtt gattttggtg
     4441 gtcaagcacc acgatggctt tgtcctttat ccgacagctc acacagatta ttcggttaag
     4501 gtcagtcctt ggaggagagg aaagggcgac ttgctccttg aagtatccca agctgccaca
     4561 gagtttgata tggatatggg ggtctacctg tcaccgtggg atgcccatag tcccctctat
     4621 catgtggacc gagaagcgga ctacaatgcc tattatctgg ctcagttgaa ggaaatctta
     4681 tcaaatccta actatgggaa tgctggtaag ttcgctgagg tttggatgga tggtgccaga
     4741 ggagagggcg cgcaaaaggt taattatgaa tttgaaaaat ggtttgaaac cattcgtgac
     4801 ttgcagggcg attgtttgat tttttcaaca gaaggcacca gtatccgatg gattggcaat
     4861 gaacgagggt atgcaggtga tccactgtgg caaaaggtga atcctgataa actaggaaca
     4921 gacgcagagc tgaactatct tcagcacggg gatccctcgg gcacgatttt ttcaatcgga
     4981 gaggcagatg tttccatccg tccaggctgg ttctaccatg aggatcagga tcctaagtct
     5041 ctcgaggagt tggtcgaaat ctactttcac tcagtagggc gaggaactcc actcttgctt
     5101 aatattccgc cgaatcaagc tgggctcttt gatgcaaagg atattgaacg actttatgaa
     5161 tttgcgacct atcgcaatga gctctataaa gaagatttgg ctctgggagt tgaggtatct
     5221 ggtccagctc tttccgcaga ctttgcttgt cgccatttga cagacggcct tgagaccagc
     5281 tcttgggcaa acgatgcaga cttgcccatc cagttagaac tcgacttagg ttttcctaaa
     5341 acttttgatg taattgagtt aagagaagat ttgaagctag ggcaacgaat cgctgctttt
     5401 catgtgcaag tagaggtgga tggtgtctgg caggagtttg gttcgggtca tactgttggt
     5461 tacaaacgtc tcttacgagg agcagttgtt gaagcacaga agatacgtgt agtcattaca
     5521 gaatcacagg ctttgccttt gttgaccaag atttcactct ataaaactcc taggttatca
     5581 caaacagtag ctgttcaggg gctagcattt gcagaaaaaa gcctagctgt ggcaaaggga
     5641 gagactcttc attttaggat tgaacgtagt gaaagtagct cctctttaga gactaagatt
     5701 tcgattcaac cggggacagg tgtccatggt gtcgcctatc aggatgagat tcaagtcctt
     5761 caattccaag caggggaaag caaaaaagat ttgcatctac caaccctgta tttcgcagga
     5821 gataaaacct tggatttcta tctgaaccta acggtggatg gtcagcttgt ggatcaactt
     5881 caagtccaag tttcataaaa gaagaacctt tgcgcgatgc aaaggttctt ttggttatta
     5941 gtgacttggt aaccagctga gggtgaaagt tagttgttca gcttttaaga ggtcttggtg
     6001 ttgaatagtt gatacgagtg ttttgtccag tcggcattct ttgacaaagt taaaatggtt
     6061 gtggttttgt ttagtatgga tatccagcca tttatcttct ttagcgaggt agactcgtag
     6121 atggtcaaag agagggattc cgaggtcata gcttggtttt cctggacagg ttggataaaa
     6181 tccgagagct gaccagatgt accaagcaga gagactacca ttgtcttcat ctccaggata
     6241 ggcttcccaa cttgggtgaa aagctttctg acggagcgtc ttgataagaa gggcagtgta
     6301 gtcagggtaa tcgctgtagc ggaagagata aggaatgtgg aaactaggct ggttggaaat
     6361 ggctatttgt ccaaaaggag cagtagccat ctcgctcatt tcgtgaattt cgtaaccata
     6421 gcctgttgtt tcaaagaggg gagcatcttg acaggctttc aaaagatagt tgctaaaggt
     6481 ttcttttcca cccatcagtt ggattaagcc agggatgtcg tggagaacgc ctaaagtagc
     6541 ttgaatggca gagcattcag cgtagtctcg cccccaacta taaagagaga agtcagggtg
     6601 aaagtttcct tgattgtctc gtgctcgcat gtaacctgtc tcagcgtcaa atagctggcg
     6661 gtaattttgt gaagcagcct tgtaggtttc agcgatttct atgtttttta gttttttggc
     6721 acagttggcg atacaaaagt cactataggc atagtctaga gtatggctaa cactttcgtg
     6781 gtggtcggta gagaggtaac ctagttcttg gtattgggct agtccgtggc ggccattgat
     6841 gccgagaggg tcggctttgc tggctgtttt gagcatggct tggaagagtt ctccttctag
     6901 gtcgggggcc atgtccttgc aggcgctatc tgcgataata ccgtctaaaa gtgtacctgg
     6961 catcataccc cgttcatctg gagccagcca ttttggaagg aaaccagtat cgcggtagct
     7021 attgaggaaa ccttctaaaa agcgttgata gtgctccggt atgataaggg caaagagggg
     7081 gaaggtggtg cggaaggtat cccagaaacc attgttgcta aagaggacac caggcttgac
     7141 agtaccagta gccagatcca tgtggatggc ttgccctgac tcatcaacct cataaaaagt
     7201 ctgtgggaag aggaagagtc tgtagagaca gtggtcaaag aaggttcggt tagcctctcc
     7261 tgtctctata acatcaaaac gatggaggag attttcccaa tccgcttggg cacttgcttt
     7321 acagctatca aaatcttctt gaggtagatt gattagagct tgagaaggag agatgaaaga
     7381 agtggctagt tgcatctcgg tttgactact tactaagtca attcgccagt ctccagcttc
     7441 ttggctgata gcaagaatat ccgtgttcat ttgtagggca gtgaacatcg ttagcgaatt
     7501 tttgttggtt tcagttttac cttcttgtcg cagggcaaga gttcgcttat ctacttgctc
     7561 tactgtcagt tcatctgctg cgtgaagata gagggagagg gctttgcctt gcttttgctt
     7621 caaacgaata gaagcaccgt agcaagtcgg tgtgagctgg gtttcaatct gatagcgcag
     7681 ggagaaaatc tttagataat gaggttggaa agaggcctta tccctatcat aggaagattg
     7741 gcggtgaaag agactgtctc ccccaagctg acctgtgaca ggtgtcagaa ggagccaaga
     7801 gtagtcccca atccaaggac tgggctggtg agttaatcga atcccctgaa agataggcag
     7861 atgcggatca aaaaaccaag atccatcctg gtcactggtc tggggcacaa agtaattcat
     7921 cccaaaaggc acgcctgtgt atggcagggt atttccccga gaaaaggcat gcttgttggt
     7981 agttccaaag cgggtatcga tggtttcaag tagtggtttc atagtctttc ctttagctgt
     8041 ttttctacat tatatcagaa aaagagggcc tttagaaaag gagtttcaaa aataactatt
     8101 ttgtagaaaa atgactatca tttgtgtatg tattttctgt ctcaaaagct atatactgaa
     8161 catgaaactt gtttgaaaga gggaattgca cgatgattta ttcgaaagaa attgttagag
     8221 aatggctaga tgaagtagca gagcgggcta aggaccatcc agagtgggtg gatgttttcg
     8281 agcgttgcta caccgatacc ttggacaata cggttgaaat cttagaagat ggttcaactt
     8341 ttgtcttgac tggggatatt cctgccatgt ggcttcgaga ttcgacagcc caactcagac
     8401 cctaccttca tgtagctaaa agagatgccc tcctgcgtca gaccattgca ggtttggtca
     8461 aacgtcagat gaccttggta ctcaaggatc cctatgctaa ctccttcaac attgagaaga
     8521 actggaaagg gcaccacgag actgaccaca cagaccttaa cggctggatc tgggagcgca
     8581 agtatgaggt ggattcactt tgctatcctt tgcagttggc ttatctcctc tggaaagaga
     8641 ctggcgagac tggtcagttt gatgagactt ttgtcgcagc gactaaggaa attctccatc
     8701 tatggacagt tgaacaagac cacaagaact ctccttatcg ttttgtccga gatacggacc
     8761 gtaaggaaga caccttggta aatgatggct ttggacctga ctttgcagtg acaggtatga
     8821 cttggtcagc ctttcgtccg agtgatgact gttgccagta tagttacttg attccgtcaa
     8881 atatgtttgc tgtagtagtc ttgggttatg tgcaagaaat cttcgcagca ttaaacctag
     8941 ctgatagcca gagtgttatt gctgatgcca agcgtcttca ggatgaaatc caagaaggaa
     9001 tcgaaaacta cgcttacacc accaacagca agggcgaaaa gatttacgct tttgaagtgg
     9061 atggcctagg aaatgccagc atcatggatg atccaaatgt accaagtcta ctagctgcgc
     9121 cctatctggg ctactgttcg gtcgatgatg aagtgtatca agctactcgt cgtaccattt
     9181 tgagccctga aaatccatat ttctaccaag gagaatacgc tagcggtctc ggaagttctc
     9241 ataccttcta tcgctatatc tggccaatcg ccctttctat ccaaggattg acaacaagag
     9301 ataaggcaga gaaaaaattc ttgctggatc agctggttgc ctgcgatggt ggtacaggtg
     9361 tcatgcacga aagctttcat gtagatgatc cgaccctcta ctctcgtgaa tggttctcct
     9421 gggctaacat gatgttctgt gagttggtct tggattactt ggatattcgc taaggggctc
     9481 gctttagctc aaccgattct tatcagaatc acaagtttac atttaaaacg ttaaaattta
     9541 aatttagaat gaggttttac ttcatggaaa atgttgttgt acatattatc tcacatagtc
     9601 actgggaccg tgagtggtac ttgccttttg aaagccatcg tatgcagttg gtggaattgt
     9661 ttgacaatct ctttgatctc tttgaaaatg accctgagtt caagagtttc cacttggatg
     9721 gacaaactat tgtccttgac gactacttac aaattcgccc tgaaaatcgt gacaaggtcc
     9781 aacgctacat tgacgagggc aaacttaaaa ttggtccctt ttacatcttg caggatgact
     9841 acttgatctc cagtgaagcc aatgtccgca ataccttgat tggtcaacaa gaagctgcca
     9901 aatggggtaa atcaacccag attggctact ttccagatac ctttggaaat atgggacaag
     9961 cgcctcaaat tcttcaaaaa tcaggcattc acgtggcggc ctttggtcgt ggtgtgaagc
    10021 cgattggatt tgacaaccaa gtccttgaag atgagcagtt tacatcccag ttttcagaaa
    10081 tgtactggca gggtgtggat ggtagtcgtg ttttaggtat tctctttgcc aactggtaca
    10141 gtaatgggaa tgaaatccca gttgacaaag atgaggcctt gaccttctgg aaacaaaaat
    10201 tgtcagatgt gcgtgcctac gcttcgacca accaatggtt gatgatgaac ggctgtgacc
    10261 accagcctgt acagaaaaat ctgagcgaag ccattcgtgt ggcaaatgaa ctcttcccgg
    10321 atgtaatctt tgttcatagt tcttttgatg aatatgttca agctgtagaa ggtgcgcttc
    10381 ctgaacactt atcaactgtt acaggcgagt tgaccagtca ggaaacagat ggctggtaca
    10441 cacttgccaa cacttcttca tcccgcattt acctaaaaca agccttccaa gaaaatagca
    10501 acctcctaga gcaagtggta gaacccttga ctattatcac tggtggacac aaccacaagg
    10561 accagttgac ctatgcttgg aaaacacttt tgcagaatgc gccacatgat agtatctgtg
    10621 gctgtagcgt ggacgaagtt caccgcgaga tggaaacgcg ttttgccaag gtcaaccaag
    10681 taggaaactt tgttaaaagt aatttgctca acgagtggaa gggtaaaatt gctacggata
    10741 aggctcaaag tgactatctc tttactgtca ttaacacagg cttgcatgat aaggtcgata
    10801 ctgtctgcac agtgattgat gtggcgactt gtgatttcaa ggaattgcac ccaacagaag
    10861 gctacaaaaa gatggctgct cttatcttgc caagttaccg tgtggaggac ttggatggtc
    10921 gtcctgtaga ggttacaatc gaagacctcg gagctaattt tgaatacgat ttaccaaaag
    10981 ataagttccg ccaagctcgt attgctcgac aagtgcgcgt gaccattcca gttcacctag
    11041 caccgctttc ttggacaacc ttccaattgc tggaaggaga acaagaacac cgtgagggta
    11101 tttaccaaaa cggagtgatt gatacaccat tcgtaacggt gagtgtggat gacaacatca
    11161 cagtctatga caagacaact cacgaagcct atgaagactt tatccgcttc gaagaccggg
    11221 gtgacatcgg aaacgagtat atctatttcc aaccaaaagg aacagagcca atctttgcag
    11281 agcttaaggg ccacgaggtc ttggaaaaca cagatcgcta tgctaaaatc ttgctcaaac
    11341 atgaattgac cgtgcctgtc agtgcggatg aaaagctaga agaagagcaa caaggtatca
    11401 tcgagtttat gaagcgtgag gctggacggt cagaagaatt gacaaacatt cctctggaaa
    11461 ctgagttgat tgtctttgtt gacaatccac aaatccgctt caagactcgc tttactaaca
    11521 ctgccaagga tcaccgtatc cgtctcttgg tcaagactca taacacgcgt ccaagcaatg
    11581 attctgaaag tatctatgag gtggtgacac gaccaaacaa accagctgct tcatgggaaa
    11641 atcctgaaaa tcctcaacac caacaagctt ttgtcagtct gtatgacgat gaaaaagggg
    11701 tgactgtatc caacaaggga ttgaatgaat acgaaatcct tggggataac accattgccg
    11761 tgaccatttt gcgtgcatca ggtgagctag gtgactgggg ctacttccca acgccagaag
    11821 cacaatgctt gcgtgagttt gaagtcgaat ttgcgattga atgccaccaa gcccaagaac
    11881 gcttctcagc ctatcgtcgt gccaaagcct tgcagacacc gtttaccagc cttcagcttg
    11941 ctagacagga aggaagcgtg gttgcgactg gtagcctctt gagccattct gttctcagca
    12001 taccgcaagt ttgtccaaca gcctttaaag tggcggaaaa tgaagaaggc tatgtcctcc
    12061 gttactataa tatgagtcaa gaaaatgtgc gcatatcaga acatcaacaa accattcttg
    12121 acttacttga gcgaccatat ccagttcatt caggactatt ggctccacaa gagattcgta
    12181 cagaattcat caaaaaagaa gaaatttaat ttcaaaaagt aaacatcaaa agaaaggagg
    12241 ggcgaaaaag taagaactaa ctgctgattc gccccttttt atggtaaaaa caatgaccat
    12301 tgcaacgatt gatatcggag ggactgggat taagtttgcc agtctgactc ctgatgggaa
    12361 aatactggat aagacaagta tttcaacgcc tgaaaacttg gaggatttac tagcgtggct
    12421 agatcaacgc ttgtcagaac aggattacag tgggattgct atgagtgttc caggtgcagt
    12481 caatcaagag acaggtgtga ttgatggctt cagtgcggtg ccctacatcc atggcttttc
    12541 ttggtatgag gcgcttagct cttatcagct acctgtccat ttagaaaatg atgccaactg
    12601 cgttggactc agtgaactac tagctcatcc agagcttgaa aatgcagcct gtgtcgtgat
    12661 tgggacaggg attggcggag ccatgattat caatggtaga cttcatcgag gtcgccacgg
    12721 tctgggtgga gaatttggct acatgacaac ccttgcccct gctgaaaaac ttaataactg
    12781 gtcgcaacta gcatcaactg ggaatatggt acgatacgtg attgaaaaat ctggtcatac
    12841 tgattgggac ggtcgcaaga tttaccaaga ggccgcagct ggtaatgctc tttgtcaaga
    12901 agccattgag cgcatgaacc gcaatttgac gcaaggcttg ctcaatatcc agtatctcat
    12961 cgatccagat gtcatcagtc tgggtggctc tatcagtcaa aatccagatt ttatccaagg
    13021 tgttaagaag gctgttgaag actttgtcga tgcctacgaa gaatacacgg tcgcaccagt
    13081 tatccaggcc tgcacctatc acgcagatgc caatctctac ggtgctcttg tcaactggct
    13141 acaggaggaa aagcagtggt aagatttata ggacttagtc ccaaacaaac gcaagctatt
    13201 gaggttttaa aaggtcacat ttctctacca gatgtggaag tggctgtcac tcagtctgac
    13261 caagcatcta tctctatcca gggtgaggaa ggtcactatc aattgaccta tcgcaaacct
    13321 caccaacttt atcgtgcctt gtccttgttg gtaacagttc tagcagaagc tgataaagta
    13381 gagattgagg aacaagcagc ttacgaagat ttggcttaca tggttgactg ttctcgaaat
    13441 gcggtgctga atgtggcttc tgccaagcag atgattgagg tcttggctct catgggctac
    13501 tcaacctttg agctttacat ggaagacact taccagattg aaggacagcc ttactttggc
    13561 tatttccgtg gagcttattc agcagaggag ttgcaggaaa tcgaagccta tgctcaacag
    13621 tttgacatga cctttgtacc atgcatccag accttggccc acttgtcggc ctttgtcaaa
    13681 tggggtgtca aggaagtgca ggagctccgt gatgtagagg acattcttct cattggcgaa
    13741 gaaaaggttt atgacctgat tgatggcatg tttgccacgt tgtctaaact gaagactcgc
    13801 aaggtcaata tcgggatgga cgaagcccac ttggttggtt tgggacgcta cctgattttg
    13861 aacggtgttg tggatcgtag tctcctcatg tgccaacact tggagcgcgt gctggatatt
    13921 gctgacaaat atggtttcca ctgccagatg tggagtgata tgttcttcaa actcatgtca
    13981 gcggatggcc agtacgaccg tgatgtggaa attccagagg aaactcgtgt ctacctagac
    14041 cgtctcaaag accgtgtgac tctggtttac tgggattatt atcaggatag cgaggaaaaa
    14101 tacaaccgta atttccgcaa tcatcacaag attagccatg accttgcatt tgcaggggga
    14161 gcttggaagt ggattggctt tacacctcac aaccatttta gccgtctagt ggctatcgag
    14221 gctaataaag cctgccgtgc caatcagatt aaagaagtca tcgtaacggg ttggggagac
    14281 aatgggggtg aaactgccca gttctctatc ctaccaagct tgcaaatctg ggcagaactc
    14341 agctatcgcc atgacctaga tagtttgtct gcgcacttca agaccaatac tggtctaacg
    14401 gttgaggatt ttatgcagat tgaccttgcc aacctcttac cagacctacc aggcaatctc
    14461 agtggcatca atcccaaccg atatgtcttt tatcaggatg ttctctgtcc gatacttgac
    14521 cgacacatga cacctgaaca ggacaaaccg cacttcgctc aggctgctga gacgcttgct
    14581 aacattaaag aaaaagctgg aaactatgct tatctctttg aaactcaggc ccagttgaat
    14641 gctattttaa gtagcaaagt agatgtggga cgacgcattc gtcaggccta ccaagcggat
    14701 gataaagaaa gtttacaaca aatcgccaga caagaattac cagaacttag aagccaaatt
    14761 gaagacttcc atgccctctt tagtcaccaa tggctgaaag aaaacaaggt ctttggtttg
    14821 gatacagttg acatccgtat gggcggactc ttgcaacgca tcaaacgagc agaaagccgt
    14881 atcgaggttt atctggctgg ccagcttgac cgcatcgacg agctggaagt tgaaatccta
    14941 ccatttactg acttctacgc agacaaggat ttcgcagcaa ctacagctaa ccagtggcat
    15001 accattgcga cagcttcgac gatttatacg acttaatact cttcgaaaat ctcttcaaac
    15061 cacgtcagct tccatctaca acctcaaaac agtgttttga gcagcctgca gctagcttcc
    15121 tagtttgctc tttgattttc attgagtata aaaacaagaa caccttgctt ggcgcagggt
    15181 gtttcgcgtg aaacagaaga attatctggt ttcaaatgct acagttaaaa ttagtaaaac
    15241 aagaaataga agctgaaaag ccagcatctg tagaggcttg gatcatttcc gtcaaattta
    15301 aaaaaggttg ctaccgacat atatagattc caaaaacaaa aacgttagcg gaactagcag
    15361 atgtgatttt atggagtttt gattttgcaa atgatcatgc tcacgcattt ttcatggata
    15421 atattgagtg gagtcatgca gattcttact ttcttagctt tgttagtgac gatgttgaag
    15481 aacgttacac agaaaatgtc tatctggata gcctaagtgt caaacaaaaa tttaagttta
    15541 ttttcgactt cggtgatgaa tggcgttttg aatgccaagt gctgagagaa atcgagacag
    15601 aggacgaaga agcttatctc gtacgttcgg ttggaacgtc gccagaacaa tatccagatt
    15661 atgatggttt tgactatgaa gaatggtaaa attgaaatca gtctgtgtag gcttagtatt
    15721 tcaatagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc agcaatcaaa
    15781 ttcattcgta atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaaac
    15841 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttggatag cgcatggtta
    15901 taggctttat cttcagctgt tagtggcttg agtttgctgg atttacgtga agtttgtgct
    15961 tgaggacata tcttcatgag cccttgataa ccactgtcag ccaagatttt accaacttgt
    16021 ctgatatttc tgcaactcat tttgaacaac ttcatatcat gacaatagtt cacagtgata
    16081 tccaaagaaa caattctccc ttgacttgtg acaatcgctt gagccttcat agcgtgaaat
    16141 ttctttttac cagaatgatt cgctaattgt ttttttaggg cgattgattt gtacttccgt
    16201 cgcatcaatc attaccgtgt cctcagagct gagaggagtt cttgaaatcg taacaccact
    16261 ttgaacaaga gttacttcaa cccattggct ccgacggatt aagttgcttt cgtggatacc
    16321 aaaatcagcc gcaatttctt cataagtgcg gtattctcac acatattgaa gagtggccat
    16381 aagaaggtct tctaggctta atttaggttt tcgtccacct tttgcgtgtt taagttgata
    16441 agctgttttt aatacagcta acatctcttt aaaagtcgtg cgctgaacac caacaagacg
    16501 cttaaatcgt gtatcagtta attgtttact tgcttcataa tttcgcaggg agtctattga
    16561 ctctttggta ggtgtcaatg tttttttcat ctatcccgag aattattttc ccgccatttg
    16621 tatttgcaaa tgctgagtag gtttcccaga aagactctgg aagattgttt ttagcttttt
    16681 tgtattctaa atcaacccct tcaaatttta agtccatatt tttcctttac atctgttttt
    16741 tgtggttctg gtatttgttc aagttgagtg ataatatagc gaattgaatt tcgagagttt
    16801 ttactcagtt aatttctttt ttaacccact ttaatttctt ttttaacacg ggttaaaaaa
    16861 gaaattaaag tgggttaatt ttccttgact taaaatttaa aaatgatata ctgtgtttat
    16921 ggagagatga caatttataa aatgtactac tctattctac tagataatga ttgaataaaa
    16981 ctttttataa caacggctag caaagttata attttatatc tattagaaaa taaatatgta
    17041 aggaaattta aatgaaaaaa actacaatat tatcattaac tacagctgcg gttattttag
    17101 cagcatatgt ccctaatgaa ccaatcctag cagatactcc tagttcggaa gtaatcaaag
    17161 agactaaagt tggaagtatt attcaacaaa ataatatcaa atataaggtt ctaactgtag
    17221 aaggtaacat aggaactgtt caagtgggta atggagttac tcctgtagag tttgaagctg
    17281 gtcaagatgg aaaaccattc acgattccta caaaaatcac agtaggtgat aaagtattta
    17341 ccgttactga agtagctagt caagctttta gttattatcc agatgaaaca ggtagaattg
    17401 tctactatcc tagctctatt actatcccat caagcataaa aaaaatacaa aaaaaaggct
    17461 tccatggaag taaagctaaa actattattt ttgacaaagg cagtcagctg gagaaaattg
    17521 aagatagagc ttttgatttt tctgaattag aagagattga attgcctgca tctctagaat
    17581 atattggaac aagtgcattt tcttttagtc aaaaattgaa aaagctaacc ttttcctcaa
    17641 gttcaaaatt agaattaata tcacatgagg cttttgctaa tttatcaaat ttagagaaac
    17701 taacattacc aaaatcggtt aaaacattag gaagtaatct atttagactc actactagct
    17761 taaaacatgt tgatgttgaa gaaggaaatg aatcgtttgc ctcagttgat ggtgttttgt
    17821 tttcaaaaga taaaactcaa ttaatttatt atccaagtca aaaaaatgac gaaagttata
    17881 aaacgcctaa agagacaaaa gaacttgcat catattcgtt taataaaaat tcttacttga
    17941 aaaaactcga attgaatgaa ggtttagaaa aaatcggtac ttttgcattt gcggatgcga
    18001 ttaaacttga agaaattagc ttaccaaata gtttagaaac tattgaacgt ttagcctttt
    18061 acggtaat
//
LOCUS       DAVLWA010000033        17316 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLWA010000033 DAVLWA010000000
VERSION     DAVLWA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.033"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            338..1384
                     /gene="pheS"
                     /locus_tag="VYN80_001112"
     CDS             338..1384
                     /gene="pheS"
                     /locus_tag="VYN80_001112"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1541032.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1384..1893
                     /locus_tag="VYN80_001113"
     CDS             1384..1893
                     /locus_tag="VYN80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541033.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1970..4372
                     /locus_tag="VYN80_001114"
     CDS             1970..4372
                     /locus_tag="VYN80_001114"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1541034.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4440..5441)
                     /locus_tag="VYN80_001115"
     CDS             complement(4440..5441)
                     /locus_tag="VYN80_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1541035.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5597..6043
                     /locus_tag="VYN80_001116"
                     /pseudo
     CDS             5597..6043
                     /locus_tag="VYN80_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6169..6435
                     /locus_tag="VYN80_001117"
     CDS             6169..6435
                     /locus_tag="VYN80_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541036.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            6696..8945
                     /gene="metE"
                     /locus_tag="VYN80_001118"
     CDS             6696..8945
                     /gene="metE"
                     /locus_tag="VYN80_001118"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1541037.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNSITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9009..9875
                     /gene="metF"
                     /locus_tag="VYN80_001119"
     CDS             9009..9875
                     /gene="metF"
                     /locus_tag="VYN80_001119"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1541038.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10560..12773
                     /gene="pnp"
                     /locus_tag="VYN80_001120"
     CDS             10560..12773
                     /gene="pnp"
                     /locus_tag="VYN80_001120"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1541039.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12789..13406
                     /gene="cysE"
                     /locus_tag="VYN80_001121"
     CDS             12789..13406
                     /gene="cysE"
                     /locus_tag="VYN80_001121"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1541040.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13418..14302
                     /locus_tag="VYN80_001122"
     CDS             13418..14302
                     /locus_tag="VYN80_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541041.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14389..15732
                     /locus_tag="VYN80_001123"
     CDS             14389..15732
                     /locus_tag="VYN80_001123"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1541042.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15725..16111
                     /locus_tag="VYN80_001124"
     CDS             15725..16111
                     /locus_tag="VYN80_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1541043.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16115..16999
                     /locus_tag="VYN80_001125"
     CDS             16115..16999
                     /locus_tag="VYN80_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1541044.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLTSW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(17046..>17316)
                     /locus_tag="VYN80_001126"
     CDS             complement(17046..>17316)
                     /locus_tag="VYN80_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541045.1"
                     /translation="KFFGLIEDNLKQVHPLFQTVFKTFLKDKKKIVNALQLPYSNAKL
                     EATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         5143 a         3365 c         3955 g         4853 t
ORIGIN      
        1 tttgttttca tcttatttca atccgctata aagttagata agaataagct aacaagcaga
       61 agtaagatag gtcttgcaag aatggtaaaa atctgataaa ctaagtggta attgaataga
      121 aatctgaaag actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc
      181 tctctatatg aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact
      241 tgtcagatga aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg
      301 gttttctttt tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt
      361 aaaagcgctt cgcgaagaaa cgctgactag cttgaagcag attactgctg gaaatgaaaa
      421 agagatgcaa gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct
      481 caaagggatg aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga
      541 agctcgtgat gtcttgacag ctgcttttga agaaacagct aagctcttgg aagaaaagaa
      601 agtcgcggct caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc
      661 gactggtcac cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg
      721 tatgggttat caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg
      781 tatgaacctt ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga
      841 agaaatcttg ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga
      901 tttttctaaa ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga
      961 cgatgcgacc cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat
     1021 ctctatggct gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga
     1081 gcgtcaaatt cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga
     1141 tgtttcttgc ttcaagtgtg gtggagaagg ctgtaacgta tgtaagaaaa caggttggat
     1201 cgaaattatg ggagccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc
     1261 gactgtatac tctggttttg cctttggtct tggacaagag cgtgtagcta tgctccgtta
     1321 tggaatcaac gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa
     1381 ataatgatta gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa
     1441 gcctttcgtg aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa
     1501 gaggcttata gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat
     1561 ttcattatgc atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa
     1621 actgagagag aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc
     1681 caaggatttg gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat
     1741 agtttttctt gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat
     1801 aatcgatatg gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat
     1861 acggattggt tactgagaaa gaaattaagg taagaagagg attcttctat tgaaatgata
     1921 ggaaaggaaa agaaccagtg tggcaatggt cattctggag aactaaatta tgcttatatc
     1981 ttataaatgg ttaaaagaat tggtggacat tgatgtgcca tcacaagagt tggctgaaaa
     2041 aatgtcaact acaggaatcg aggtagaggg tgtcgaatca ccagctgctg gtctctcaaa
     2101 aattgtcgtc ggtgaggtct tgtcttgcga agatgtgcca gagactcacc tccatgtttg
     2161 tcaggttaac gttggcgaag aagagcgtca gatcgtttgt ggtgccccaa atgtgcgtgc
     2221 tgggatcaag gtcatggtgg ctcttccagg agctcgtatc gctgataact acaaaatcaa
     2281 aaaaggaaaa atccgtggtt tggagtcact tggaatgatc tgttcacttg gtgaattggg
     2341 aatttctgac tcagttgtgc ctaaggaatt cgcagatggc atccaaatct tgcctgaaga
     2401 tgccgtgcca ggtgaggaag tcttttctta cctagacttg gatgatgaaa tcatcgaact
     2461 ttccatcaca ccaaaccgtg cagatgccct ttctatgtgt ggagtggctc acgaagtggc
     2521 agccatctat gacaaggcag tcaactttaa agaatttact ctaacagaaa ctaatgaagc
     2581 tgcggcagat gccctttctg tcagcattga gacagacaag gcgccttact atgcagctcg
     2641 tatcttggac aatgtgacca tcgcaccaag tccacaatgg ttgcaaaatc ttctcatgaa
     2701 cgaaggaatc cgtcccatca ataacgtagt ggacgtgacc aactacatcc tgctctattt
     2761 tggtcaacca atgcatgcct ttgacttgga taactttgaa gggactgaca tccgtgtgcg
     2821 tgaagcgcgt gctggtgaaa aattggtgac cttggacggt gaagaacgtg acttggacgt
     2881 gaatgaccta gtcatcactg tcgcagacaa gccagtagcc cttgcaggtg tcatgggtgg
     2941 tcaagcaaca gaaatctctg aaaaatctag tcgtgttgtc cttgaagctg ctgttttcaa
     3001 tggcaaatct atccgtaaga caagtggtcg cctgaacctt cgttctgagt catcttctcg
     3061 ctttgaaaaa ggaattaatg tggcaacagt taatgaagcc cttgatgcgg cagctagcct
     3121 gattgcggaa cttgcaggtg cgacggtgcg taagggcatc gtttcagcgg gtgagcttga
     3181 tacttcagat gtagaagttt cttcaaccct tgctgatgtt aatcgtgtcc tcggaactga
     3241 gctgtcttat gctgatgtag aagacgtctt ccgtcgtctt ggctttggtc tttctggaaa
     3301 tgcagacagc tttacagtca gagtcccacg tcgtcgttgg gatatcacaa tcgaagctga
     3361 cctctttgaa gaaattgctc gtatctatgg ttatgaccgc ttgccaacta gtctaccaaa
     3421 agacgatggt acagcaggtg aattgacagc cacacaaaaa ctccgccgtc aagttcgtac
     3481 tattgctgaa ggagcaggtt tgacagaaat catcacctat actctaacaa ctcctgaaaa
     3541 agcagttgag tttacggctc aaccaagtaa ccttacggaa ctcatgtggc caatgactgt
     3601 ggatcgttca gtcctccgtc aaaatatgat ttcaggtatc cttgataccg ttgcctacaa
     3661 cgtggctcgt aagaataaaa acttggccct ttacgagatt ggaaaagtct ttgaacaaac
     3721 aggtaatcca aaagaagaac ttccaaatga aatcaacagt tttgcctttg ccttgacagg
     3781 cttggttgct gaaaaagatt tccaaacagc agcagttcca gttgatttct tctatgctaa
     3841 gggaatcctt gaagccctat ttactcgttt gggactccaa gtaacctata cagcaacatc
     3901 tgaaatcgct agccttcatc caggtcgtac agccgtgatt tcattcggtg accaagttct
     3961 tggtttcctt ggccaagtgc atccagtcac tgccaaggct tacgatattc cagaaacgta
     4021 tgtggctgag cttaaccttt cagctatcga agctgcgctt cagccagcga ctccatttgt
     4081 agaaatcacc aaattcccgg cagtcagccg tgacgttgcc cttctcctca aggcagaagt
     4141 gactcatcaa gaagttgtag atgctatcca agctgccggc gtgaaacgtt tgacagatat
     4201 caaactcttt gacgtcttct caggtgagaa attgggactt ggtatgaagt caatggctta
     4261 tagcttgacc ttccaaaatc cagaagatag cttaacggac gaagaagtcg cacgctatat
     4321 ggaaaaaatc caagcatcgc tcgaagaaaa agtcaatgca gaagtgcgtt aaacgatagt
     4381 atgatacaaa ttccccctct agtttatgaa cactagaggg gtgattttat ataaaataat
     4441 taaaatgtga tttctgtaaa aatacatcta tgatcagagt tttgaaaact tacaatgtct
     4501 aaatctttaa catagtagtg gtttttaggc aataaaatat gatctattgt tgcattaaaa
     4561 agttttggac tttggctatt ccaagttcct ctttcaaaag gtggcagtac atttaatgcg
     4621 tccctatgag agcttatttt tgcaagtgct ccatgacgca tagttgcatt aaaatcacct
     4681 gcaataatag cctttggata ttttgaagcc aattgattat gaatgatgtt taagtcttgc
     4741 ttccagattt ccattaaacc tggcagagga ggagcagtat gcaaggcaat gatatctggt
     4801 atattttgtt ttctcgaatg taatacaatt gtcccgaacc gtgttgtatg aaaagtttta
     4861 gcttctgtat agaaaccata acttttcttg acaatcacag tcacaggagc tattccacta
     4921 ttggtaggtg gagaagtgaa aatatcatag ttggccatag aaagtccaac ttgatgaaac
     4981 aatagtttga ttctctggtt ttcttgctca cctctgatat tggtagctag ttcagggaat
     5041 atagccatat cggcgtcaaa atggctaaaa attcgctcaa tatgttgtgc ttctatttga
     5101 ttagcgacgt tccaagttac taattttaag gttttagtct tatttgatgc agaagaaagg
     5161 gttttatctg ttccgaataa tagtaatatg agcgatagta agagcaaaat gccagagtaa
     5221 aaccgtattt gtttattttt cttgtaaaaa gatagggaaa aaaatacgaa acttaaaata
     5281 gcagccatag ttgcagtagc taaggggaag gcataaatct gtgccaagat aggttgggta
     5341 gatagccaaa tgttaagatt agaagatggg aaaaaagaat aagttataat agggatagaa
     5401 aagagaatga ccgaaagggc agagagaaat gttttaatca ttgtgtgagt acctcaattt
     5461 gcaatggttg ctcggagcta atgtgacttt gtgactttac ttgattgata ttctttttct
     5521 tgtcttcctc atagattaag tacaacccaa gtaaaaaaag tccaatttaa gattgaaatg
     5581 attaaaaggc acagaaatgc aagacaatta tactacaaaa ggcaaacatt tgacaatcga
     5641 tagccgtcgc ttaatcgaac gatggaaaaa agaaggaaaa tcaaatagag aaattgcctc
     5701 tctacttgga aaagctcctc aaactatcca tactgaaatc aagtatggga cagtccgaca
     5761 atgtcttgga aaagggcgct tcaaagagat ttattctgcc gactacgctc aacagtctta
     5821 tgaaaataat cgcaagcact cggtcaagaa atcaagcttg actaagaaac taaaggaaaa
     5881 gattctccac tatcataacc aaaaattttc gcctgaaatg atggtgatgg ctaaaggggt
     5941 taacgtggga atttcaacca tttactattg gattcatcgt ggaaaattgg ggttaagcaa
     6001 gcaggatctg ctttatccta gaaaaggaaa agcgcttaag aaataggcta gcaccaactt
     6061 taaacccgct ggtcaatcta tcgaatcata attcgctcat ttttttaaaa atacatagac
     6121 gtaaaaaaaa gagggttaag tgatgaagaa tagtgctatt gggagtaatt ggaaggatgt
     6181 ccatctgagc tctttagcaa ggaggaaatc cttgaaagtg atatgcgagt ggctatcatg
     6241 agcgagttga ttgaggctag gtatgaacaa ggaatcagtc agaaaaagct ggaagaagtc
     6301 agtggaataa gccagcctgt catagctagg atggagacag gagagagcag tcttcagttg
     6361 gatacggtct taaaagttct aaccagtcta ggaaagacac tagcagtcgt tccacttgaa
     6421 caggggaaaa gttgactaga atcagttata gtcttttcta ataacaggtc atagtcactt
     6481 ataagaatta ctaataacat gttttagtat tctaattgga atatagctgt aaagtaagaa
     6541 ttacaaagga tttgagacgt ttgcttcaag tctttttctt ttgtccgaaa cagatatata
     6601 gtttcaaact atatcaaacc ctaatttttt acaattatac agacgctttc ttttctgtta
     6661 agatagtttc aacaacaaat tttggaggac acatcatgtc aactacaatc atcggtttcc
     6721 ctcgtttggg cgaattccgt gaattaaaat ttacaactga aaaatacttt agaaaagaaa
     6781 tctcagaaga agaactttta gcagcagcaa aagacttgcg tgctaaacac tggaacattg
     6841 tcaaagaaaa aggcatcact gaaattccat caaatgactt ttctcactat gataacttcc
     6901 tagatgcggc tttccttttc aacgtggtgc ctgcatccgt tcaaaacttg gacttgtctg
     6961 accttgagcg ctacttcgct ttgggacgtg gttaccaggg agaaaaaggg gacgttcgcg
     7021 cccttccaat gaagaaatgg ttcaacacta actaccacta catcgttcct aaatttgaaa
     7081 aagacactca agtaaaattg gctggtcaca agattttcga tgagttccaa gaagcaaaag
     7141 aacttggatt gaacactcgt cctgttcttg taggtccatt cactttcctt caattgtcag
     7201 actttgaaga aggcgtgaaa gcagacgact tcgtagatag cttagtggtt gcttaccaag
     7261 aagtttttgc taaattggct gaacttggtg cgactcgcat ccaattggat gaagcggctc
     7321 ttgtaaaaga cttgacagct gaagaaaaag ctctcttctt gaacctttac aacaaactct
     7381 tggctgacaa aaaaggtctt gaagtcttgc ttcaaactta tttcggtgat gttcgtgacg
     7441 tatacgctga ccttgtaaac ttgccagtag atgctatcgg tcttgacttt gttgaaggta
     7501 agaaaactct tgaactcgtt aaaggtggct tcccagctga caagactctc tatgtaggta
     7561 ttgtcaatgg taaaaacatc tggcgcaaca actacgaaaa gagcttggct gttcttgagc
     7621 aaatcccagc tgaaaacatc gttttgacaa gctcttgctc acttcttcat gtgccattta
     7681 caactgctaa tgaagaattt gaaccagcac tcttgaacca ctttgccttt gcagttgaaa
     7741 aattggatga aatccgtgac ttggatgcta tccgcaatgg tcaaggttca gaagctcttg
     7801 cagccaacaa agaactcttt gcgactgagc gtgttggtga aaatgctgaa cttcgtgcgc
     7861 gtatcgctgg cttgacagac gcagactaca ctcgtttgcc agcctttgca gaacgtgaag
     7921 ctatccaaga agaagctttc aaacttccag ctcttccaac aacaacgatt ggttcattcc
     7981 ctcaaacaaa agaagttcgt gccaaacgtt tggcttaccg taaaggtgaa ttgtctcaaa
     8041 aagagtacga tgctttcctt gctgaaacga ttgacgaatg gatcaagtgg caagaagata
     8101 ttgactttga tgtccttgtt cacggtgaat ttgagcgtaa tgacatggtt gagtacttcg
     8161 gtcaaaactt gtcaggatac ctcttctcta aaaatggatg ggtacaatca tacggtatgc
     8221 gtggggtaaa accaccaatc atctggggtg atgtcactcg tcttaactct atcactgtta
     8281 aatggtctag ctatgcacaa agtcgtacaa acaaacctgt taaaggtatg ttgactggac
     8341 ctgttaccat ccttaactgg tcattcccac gtgaagacat ctctatcaag gattcaactc
     8401 ttcaaatcgc ccttgctatc aaggatgaag tacttgacct tgaagctgct ggtgtgaaaa
     8461 tcatccaaat cgacgaggct gctcttcgtg aaaaattgcc actccgccgt agcgactggt
     8521 acgaagacta ccttgactgg gctattcctg ccttccgctt ggtacactca acagtagcgc
     8581 cagacacaca aatccacact cacatgtgtt actcagaatt tacagatatc atcccagcta
     8641 tcgataacat ggatgcagac gttatttcct ttgaagctag ccgttcaaac cttgaaatct
     8701 tggacgaact caaagcgaaa aacttccaaa cagaagtggg acctggggtt tacgatatcc
     8761 actcacctcg tgtaccaaat gaaggcgaaa tcgacaacac aatcgaagcc atccttgcta
     8821 aagtgccaag caagaaagtt tggatcaacc ctgactgtgg tttgaaaaca cgtggtatcc
     8881 cagaaacaaa agaaagcttg atccgccttg ttgaagcagc taaagctgcg cgtgagaaat
     8941 tgtaagattg gatttctctc catacagtgg ttgttcaaaa agaaatcaac tagaaaaggt
     9001 tattacatat gtcacgccaa acaccgtcac tctcatttga agtgttccct ccaaacccag
     9061 ccgtgggtaa tggtaacatt atttcagcct tgcaggatat gcaggagttg gctccccatt
     9121 ttatcagtgt aactgccagc aataataaat ttaatatcaa ggaaacgacg gttcgtttgg
     9181 ctgactttat ccaaaatgac ttggcgattc cgaccattgc ccacttgcca gctatttatc
     9241 taactaagga caaggttgct gaaactattg ctgacttgga caaagttggg gtgcagaaaa
     9301 tcttggctct tcgtggggat attattccag acgtagaacc acaaaaggat ttccgctatg
     9361 caaccgactt gattgagttt atcaaggaac aagcccctca ctttgatatt attggagctt
     9421 gctatccaga agggcatcca gattcgccaa atcagatttc agatattcaa aatcttaaga
     9481 agaaagtgga tgcaggctgt tcgagcctcg taactcagct cttctttgat aatgagcgct
     9541 tctatgattt tcaggacaag tgtattttgg ctgggattga tgttcccatt catgcaggaa
     9601 ttatgccaat tctgaatcga aatcaggctc tccgactctt gaagacttgt gagaatatcc
     9661 atcttccacg caaatttaaa gccatcttag acaagtatga gcatgaccct gagtcgctca
     9721 gagcagcagg acttgcctat gcagtggacc aaatcgtgga cttggtaact caggatgttg
     9781 ccggtgtgca tctctatacg atgaataatg ctgatacagc aaaatacatc catcaagcaa
     9841 cccatgcctt gtttaatcac cagtctctag gataataaaa gcaaaccatt cttctcaggt
     9901 gaggggaatg gttccttttt aatggtaaag acctcacttt ttaagaaaaa tatgataaaa
     9961 taaattctgt acgtacttga tacaaagatg aaggtattaa ataatacaat gcatttaaac
    10021 tgtaagattt aacgacacga gtgagtcaaa tcgatgttat ttgacgagag agttagtaaa
    10081 gcatttagct aatcgcccga tagcgattag cgtgaaagct ttagatactt tagtattaaa
    10141 cctttctaat gatgtatctt gaaaactgaa cttgcctaca actctgtgga aaaagataaa
    10201 tctccctagg agcagtcgct ccgtcgttcg atttcctatt ttcctttgag ttgcttgacg
    10261 gcttggtatc ttgatgactt gaattttggt ctcatttctt aggaaaaaag ataagcttcc
    10321 tagcactcgt cgagtgcgac gtcaccttcc tatttttcta cagaaatgtt cggcaagccg
    10381 aaccgtccaa aatatcttgt gtaattgaac acggccgaaa agctgtgtaa aaaagataaa
    10441 ctgtcttgtc ttcatcgaag acttcgtcag tttcctattt ttactttgct tttgacgtcc
    10501 ttggtatctt gatctttgta ggcaaggcgt ataatttcat caatccaaag gggattaaaa
    10561 tggcaaaaca agtgtttcaa acgacttttg cgggtcgtga gttaattgta gaaactggtc
    10621 aggttgctaa gcaagcaaat ggctctgttg tcgtacgtta cggtgagtca actgtcttga
    10681 ctgctgccgt tatgtctaag aaaatggcaa ctggggattt cttcccactc caagtcaact
    10741 acgaagaaaa aatgtatgcg gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac
    10801 gtccttcaac agatgcgacc ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt
    10861 ttgcggaagg tttccgtaat gaagtccaag tcatcaatac agtgctttct tatgatgaaa
    10921 atgcatctgc accaatggct gctatgtttg gttcatcttt ggcactgtct atttcagata
    10981 ttccatttga cggaccaatt gctggggtac aagtgggata tgtagatggc caaatcatca
    11041 tcaacccaag tcaagaacaa gcagagcaat ctcttcttga attgacagta gctggaacca
    11101 agcacgctat caacatggta gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg
    11161 aagcgcttct taaagggcac gaagctgtca aagaattgat tgccttccaa gaagaaatcg
    11221 ttgctgctgt cggtaaagaa aaagcagaag tggaattgct tcacgtggat gctgaattgc
    11281 aagctgaaat cattgcagcc tacaacagtg acctccaaaa ggcagttcaa gtagaagaga
    11341 aattggcccg tgaagctgca actcaagcag tgaaagacca agtgactgcc gtttacgaag
    11401 aaaaatatgc gaaccacgaa gaatttgacc gtattatgcg tgatgtggct gaaatcttgg
    11461 aacaaatgga acacgcagaa gtgcgacgtt taattacaga agacaaggtg cgtcctgatg
    11521 gtcgtaaggt cgatgaaatc cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac
    11581 atggttcagg tctctttact cgtgggcaaa ctcaagctct ttcagtcttg accttggctc
    11641 cgatgggaga aactcaaatc attgatggtt tggatccaga gtacaagaaa cgctttatgc
    11701 accactataa cttccctcaa tattctgtag gggaaacagg tcgttacggt gcgccaggtc
    11761 gtcgtgaaat cggtcacggt gcccttggtg agcgtgctct tgctcaagtc ttgccaagct
    11821 tggaagaatt cccatacgct atccgtctag tagcagaagt tttggaatca aacggttctt
    11881 catctcaagc ttctatctgt gcgggaactc ttgcccttat gactggtggt gtgccaatca
    11941 aggcgccagt agctggtatt gctatgggac ttatctcaga tggaaataac tacacagtat
    12001 tgacagatat ccaaggtttg gaagatcact ttggagatat ggacttcaag gttgcaggta
    12061 ctcgtgatgg gattacagcc cttcaaatgg atatcaagat tcaagggatt actgcagaaa
    12121 tcttgacgga ggctcttgct caagccaaga aagcgcgttt tgaaatcctt gatgtcattg
    12181 aagcaaccat tccagaagtt cgtccagaat tggctccaac tgctccgaaa attgatacga
    12241 tcaagattga tgtggacaag attaagattg tcatcggtaa gggtggagaa accatcgaca
    12301 agattatcgc tgaaacaggt gttaagattg atatagacga agaaggaaat gtgtctatct
    12361 actctagtga ccaagatgct attaaccgtg ccaaagaaat tattgctggt ttggttcgtg
    12421 aagccaaagt ggatgaagtt taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct
    12481 ttgttaacct ctttgataag acagatgccc ttgttcatat ctctgagatg gcttggactc
    12541 gtaccaatcg tgtagaggat ttggtagaaa tcggggatga agttgatgtt aaggttatca
    12601 agattgatga aaaaggccgt atcgatgcct ctatgaaggc tcttctacct cgtccgccaa
    12661 aacctgagca tgatgaaaaa ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca
    12721 aggatcacaa acctaagaaa gaatttacag aaacaccaaa agattcagag taagaaaagg
    12781 agaaatgtat ggggtggtgg cacgaaacca ttgatatcgt aaaagaaaat gatccagcgg
    12841 cccgcaccac tttggaggtt ttgctgactt atccaggtgt caaggccttg gcggcccacc
    12901 gtctctcgca ttttctctgg aagtacgact tcaaactctt agctcgtatg cacagtcagt
    12961 tttggcgctt ttggactcag attgagattc atccaggagc ccagattgat tcaggtgtct
    13021 ttattgacca tggttctggt ctggtgattg gagagacagc gattgttgaa aaaggcgttc
    13081 ttctctatca cggagtgact ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga
    13141 ctgtacgaaa gggagccctc atatcagccc atgcccaagt tatcgggcct gtggaaattg
    13201 gtgaaaatgc caaggtcggt gctgcagcag ttgtcgtagc agatgtacct agtgatgtga
    13261 cggttgtcgg tattccggcc aagattgtcc gtcttcatgg taagaaagat gagcctgtta
    13321 ttcacgaagt cgaagaaaaa cgggagtatt atgtcaataa actcgagcag gctaaagatg
    13381 ccagtcacag atcgtctggt ttgtagagga tacctatatg attcaagcaa gaaacaagtt
    13441 aagccaagag gagctatctg aggcgaaaaa agtaattaac tgttgccaaa actatgacgg
    13501 tacctatcgc gatccctatc tctctaacat gcttaatttt gacccaaaca tgcccgcctt
    13561 tttcctttat tatgaaaaag gcaaacttgt tggtttatta actgtctatg cagatgacca
    13621 agatgtagaa gtgacgatat tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc
    13681 attgtttact agttttgaga gagaaacagc ttctttcccc attcggtcag tcacttttca
    13741 gacagaacgt atttttttag agaatcatcc tgactttgcc agtaactggg gactaatcga
    13801 ggatgaagag acagaaactt ggttaggtaa ggatagaaga ccttatccgt tagcaaatgt
    13861 ttcccatctt gaagttttgt tagcagatag ttcgtatcag gatcaaatta gtcaattaaa
    13921 atttcaggca ttttcagagg aacatgaatc gagagaagtt gtggatagat atgtcgctga
    13981 agctctgaag gatccagaaa gtcgcttata tattttgtta aaagacggtc aggttattgg
    14041 gacttgcacg gtagatttat cgactaatac gaattacttc tacggtttag caatatcgga
    14101 acctgaacgt gggaaaggct atggaagcta cttagcaaaa tccctcgtca accaactaat
    14161 tgagcaaaat gacaaggaat ttcagattgc agtggaagat agcaatgtag gtgccaaacg
    14221 tttgtatgaa aaaattggct ttgtcaaaca gacacaggtg gtttatctga atgagaaagg
    14281 agcaagggat tccgaagtgt agagacattc ggactgaaat ttacttgagc tcttagttta
    14341 gtgatgaaac taacagttct tggattttag ctttcctgat tatgatttat gattaaaatc
    14401 tatgacacca tgtctcgtga tttgcgagaa tttgtcccga ttgaggacgg caagatcaag
    14461 atgtatgttt gtgggccaac cgtgtacaac tatattcacg tgggaaacgc ccgttcgacg
    14521 gtagcttttg atacgattcg tcgctatttt gagtaccgtg ggtataaggt tgcctatatt
    14581 tccaatttta cagatgtgga tgataagatt atcaaccgtg ccagggaaga aggcatcaca
    14641 cctcaggagg ttgcggataa gtacatcgct gcctttcgtg aggatgtgac ggccttaggc
    14701 gtgaaacctg cgactcgcca tccgcgtgta gtggagttta tggcagacat catccgtttt
    14761 gtggaagact tgatcgagaa aggctttgcc tatgagagtc aaggggatgt ctatttccgt
    14821 gtagaaaaat cccacaacta tgctaaattg gctaataaaa ccttggaaga tttggagcta
    14881 ggtgcttcag gtcgtaccga tgaagaaacg gctcgtaagg aaaatcctgt agactttgct
    14941 ctatggaaat cttccaaacc aggtgagatt tcttgggata gtccttgggg acctggacgt
    15001 ccgggttggc atattgagtg ttcagtcatg tcgacagaga ttttaggtga taccattgat
    15061 atccacggtg gtggagctga cctagagttt ccacaccaca ccaatgaaat tgcccaatct
    15121 gaagctaaaa caggcaaggc ttttgctaac tactggatgc acaatggctt tgtcaatatc
    15181 gacaatgtca aaatgtctaa gtccttgggt aacttcatta cggtacacga tgcccttaaa
    15241 actcttgatg ggcaagtact tcgtttcttc tttgcgaccc agcactaccg taagcctatc
    15301 aacttcacgg aaaaggcagt tcgcgatgcc gagaccaatc tcaagtatct gaagaatact
    15361 tacgaacaac catttacagg aaacgtggat gcccaagagt tacaaaactt taaagataag
    15421 tttgtagcag ctatggatga agattttaat gctgccaacg gtatcacagt tgtctttgaa
    15481 atggccaaat ggatcaactc agggaactat gatgcaagtg tcaagcaagc tcttgcagat
    15541 atgttagaaa tttttggaat tgtctttgtt gaggaagttt tggatgcaga gattgaagac
    15601 ttgattcaaa aacgccaaga ggcgcgtgcc aatcgtgact ttgagacagc agaccaaatc
    15661 cgtgaccaat tggttactca aggaattaag ctccttgata ccaaggatgg agtgaggtgg
    15721 acacgtgatt gatgtcaatc tcattaacgg gattgcgcta gcctttgagg gagatgcggt
    15781 gtattctatg tatattcgcc gccacctcat cctcaaaggt atgaccaagc ccaataaact
    15841 ccaccaagag gcgaccaagt acgtgtcagc caaggctcag gctcgtctga ttgctctcat
    15901 gttggaggag cagatcctaa cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac
    15961 caatagtcac acaaaggcta aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt
    16021 tgaagcagtt atgggctatc tccatatgac tgagaatctg gaacgtcttg agagtttggt
    16081 ttcatggtgc atccaaaaag tggagggcta gaacatgagg gcaaaagaac tacaagactg
    16141 gtttcctgag gctcggattt cagaccaacc agtagagaaa gagggctatc tcacgcttcc
    16201 tttagcttct cagcagtgga ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca
    16261 gttggttgcc cttttgaccc agcaggagca ggctcgttcg ctaaaccctt ggtattccta
    16321 tctggttgag ggcaagggac aggcaccgca agtttttaaa aagattcagt tggtttattg
    16381 ccatctttct tattttcagc aggaaaatct gacttcttgg ctagatatga tgcggactct
    16441 ttttccgaat tgtcagacag tgctacaggt cggagctcag gattatgttt tcgtgcttca
    16501 acaagacaaa tacacttctg taagagatat tttgagtgat acgattgaag cggttgagta
    16561 tgactttgga cttcgtcttt ctatcatgtt gggtcaggtt tggtctcaga caggacatca
    16621 agccctatca gacttaatca aagctgagcg ggatttgttc aagacatggt ggcgtcaggg
    16681 tcaccaaggt gttcatactt tttctcagct ctatctttgg agtatgggag aaagactcgt
    16741 ggacttgaag ccaattaaag aatgtctaca ccagatgatt ttggatcaag atcagattca
    16801 ggaaatcatt ctctctcttt gggaaaatag tgctgttctc actaaaacag cccagcaact
    16861 ctatctgcac cgcaattctc tccaatacaa gattgataaa tgggaagagt tgacagggct
    16921 tcagttgaaa gagttgaccg acctgacctt gtgttatcaa ttgattttag gttctttgtc
    16981 aactatagtt ggtttgtaaa gaagttaata tggctctttg tcaactgtag tgggttgaaa
    17041 aaaagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcgata
    17101 aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg tttgataagt
    17161 ttgatgagat tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg
    17221 acgattttct ttttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc
    17281 tgcttaagat tgtcctcaat aagtccgaaa aatttc
//
LOCUS       DAVLWA010000034        16901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLWA010000034 DAVLWA010000000
VERSION     DAVLWA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.034"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..337
                     /locus_tag="VYN80_001127"
     CDS             <1..337
                     /locus_tag="VYN80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541046.1"
                     /translation="GTTFVEANQIGAFSNFVITTSYKRTGYLTKENEGAEYIMNLNPC
                     RNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWATVGYVYAVDMARQNWWDLSA
                     AISGSWSPN"
     gene            359..1150
                     /locus_tag="VYN80_001128"
     CDS             359..1150
                     /locus_tag="VYN80_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541047.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            1143..1784
                     /locus_tag="VYN80_001129"
     CDS             1143..1784
                     /locus_tag="VYN80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541048.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            1788..2438
                     /locus_tag="VYN80_001130"
     CDS             1788..2438
                     /locus_tag="VYN80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541049.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            2449..2736
                     /locus_tag="VYN80_001131"
     CDS             2449..2736
                     /locus_tag="VYN80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541050.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            2882..5395
                     /gene="secA"
                     /locus_tag="VYN80_001132"
     CDS             2882..5395
                     /gene="secA"
                     /locus_tag="VYN80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1541051.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5476..6507
                     /locus_tag="VYN80_001133"
     CDS             5476..6507
                     /locus_tag="VYN80_001133"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1541052.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6509..7540
                     /locus_tag="VYN80_001134"
     CDS             6509..7540
                     /locus_tag="VYN80_001134"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1541053.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7579..7947
                     /locus_tag="VYN80_001135"
     CDS             7579..7947
                     /locus_tag="VYN80_001135"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1541054.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            7937..9040
                     /locus_tag="VYN80_001136"
     CDS             7937..9040
                     /locus_tag="VYN80_001136"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1541055.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9059..11074
                     /gene="recG"
                     /locus_tag="VYN80_001137"
     CDS             9059..11074
                     /gene="recG"
                     /locus_tag="VYN80_001137"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1541056.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWCMIALHLEKKEHLD"
     gene            11390..12370
                     /locus_tag="VYN80_001138"
     CDS             11390..12370
                     /locus_tag="VYN80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1541057.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(12724..12867)
                     /locus_tag="VYN80_001139"
     CDS             complement(12724..12867)
                     /locus_tag="VYN80_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541058.1"
                     /translation="MADRTDEVSSKHRFEVADRTDEVSSKHRFEVADRTDEVSNIYTA
                     RRS"
     gene            13264..13491
                     /locus_tag="VYN80_001140"
     CDS             13264..13491
                     /locus_tag="VYN80_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541059.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            13779..16724
                     /locus_tag="VYN80_001141"
     CDS             13779..16724
                     /locus_tag="VYN80_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1541060.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSEEGENITLPAEHVESVSETELSGNKQEQERKDK
                     QEEKIPRDYYARDLENVETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFK
                     PDAKAPAFYNLFSVSSATKKDEYFTMAVYNNTATLEGRGSDGQQFYGNYNDAPLKVKP
                     GQWNSVTFTVEKPTAELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRA
                     NNTVWGSNLQIRNLTVYNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESG
                     RNGKPNKDGIKSYRIPALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWG
                     DKVVISNLRDNPEAKDPAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTP
                     EKAYEKIGDKTYQILYKQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGN
                     LYKGQELIGNIYFAHSTKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKF
                     LGTGPGTGIVLRNGPHKGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVN
                     DNRQVDGQKIHSSTMNNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTW
                     EKDIKRYPQVKDVYVQMSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTW
                     LKHNPIQKGEFAYNSLQELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKNLISPT
                     EAKVKRTREMGKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFA
                     VDKEDIGQEIIGIAKGSIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGIE
                     PAVHEIAEYKGSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGL
                     FTLGKKREQ"
BASE COUNT         5101 a         3065 c         3831 g         4904 t
ORIGIN      
        1 tggaactact ttcgtagaag caaatcagat aggtgctttt agtaattttg ttattactac
       61 cagttataag agaacaggtt atttgactaa agaaaatgaa ggtgcggaat atatcatgaa
      121 cttaaatcct tgtagaaatt tacatcccat gactgttaag catcgtatag taaattctaa
      181 tggagaagct cgtagtggag aatcgttaac aacctgtggt actcgttcaa ctcatggtaa
      241 ttgggcaacg gttggctatg tttatgcagt agatatggca agacaaaatt ggtgggattt
      301 atcagctgcc atttcaggta gttggtcacc aaattaattc gtaaagtagg tataatttat
      361 gaagattcct ctcttaactt ttgcaaggca taaatttgtt tatgtcttgc ttactttgct
      421 ttttcttgct ttggtttatc gtgatgtttt gatgacttat ttcttttttg atattcatgc
      481 gcccgatcta gctaaattcg atggacaagc aattaaaaat gacttattaa aatcagcatt
      541 agattttcgt attctccagt tcaatctagg tttttatcaa tcatttatta ttccaatcat
      601 cattgttttg ctaggttttc aatatattga gctgaaaaat aaagttttac gattgagtat
      661 tggaagagaa gtgagctatc aagggttaaa aagaaagttg actttgcaag ttgcaagtat
      721 cccttgtttg atatatttag tgactgtgct gataattgca attataacct atttctttgg
      781 gactttttct cctcttgaat ggaattctct attttctgat gggagtggtt tacaaagact
      841 cctagatgga gagataaaaa gctatttgtt ctttacttgt gtcctattaa tcggtatttt
      901 catcaatgca atctattttt tacaaatagt tgattatgtg gggaatgtga ctcgttcggc
      961 aatcacctat ttgatgtttc tttggcttgg ttctatgctg ctctatagtg ccttgcctta
     1021 ctatatggtt cctatgacga gtttgatgca agctagctat ggggatgtaa gtttgatgaa
     1081 actctttact ccttatatcc tttatgttgt cccttatatg gtacttaaaa aatatgaaga
     1141 taatgtttaa gaattttaac aatatttggc taaagagaaa gatcgtttta ctacttcgta
     1201 tagttctgat gatgattttg ataaactatc tattgtcaac agcggttcaa aagcaggatg
     1261 ctgttatctt tttcaagaga gaattgattt caattttttc ctataatgac tattctgaag
     1321 cgaatttaga aatccccaaa ctcttgttaa acctttcgct tttcatggta ggatggctct
     1381 ctgtcatttt acttgaaagt gatttggcag accattacca tcacttgatt cgctatcaat
     1441 caagctcctt tttcgattat acaaggaaac gattggttgt catttctaaa ttttttactc
     1501 aagatttgtt tgtctggttt cttggtttac ttccgctagg aattcatttc aaaacagtcg
     1561 cacttttctt tttacttgct cagttaatga tgttgtactt actactgtct tatctgatag
     1621 cactgattag tgcgggcgct ggtttttctt tttttctcta ttttttggca tttgtgggac
     1681 aagaatggat gatggatcat attgtaacag tgtatttagt actcttaagc ttattagtta
     1741 tgttgattct tagtcgcttg gaagagaaat ttaagaaagg ataaacgatg agacttgaaa
     1801 ttataaatgg acagaaaatt tatgggaaaa gacctatttt aaatcagttg aatttggtgt
     1861 ttcaatcagg aaaaatttat ggacttaaag gtgataatgg atctggcaag acggttcttt
     1921 taaagatact tgctggttat attaagcttg acaaaggaaa agttcttcaa gatggtaaag
     1981 tttacggggt aaaaaatcat tatattcagg atgcaggaat tttaattgaa aaagtcgagt
     2041 ttttatctca tttatccctg agagaaaatt tggaactgtt aaggtatttt tcatctaaag
     2101 ttacggaaaa aagaattgcc tattggattc aatactatga tttacaggaa tttgaagacg
     2161 ttgaataccg tcatttatcc ttgggaacaa agcaaaaaat ggccttgatt caagccttta
     2221 tttcctctcc ttctatactc tttctcgatg aacctatgaa tgctttggat gagaaaagtg
     2281 tgaggttaac caaacaggtc attttatctt acctgaaaaa agaaaatggt ctggttatcc
     2341 tgacgtcgca catatcggaa gatatttcag acctttgtac agatgtatta gttgtcgaaa
     2401 atggacatat acaatatgta aaggatatac aatcctagga gatggcttat ggcacatcta
     2461 aaatcattta ttacacgcta ttccaaggtt tatattggtt tagttctgct gatctggctg
     2521 tctttcttct ttatcccttg ggatagagcg attctggggg taaggattga catcttcatc
     2581 atacagaaag tcttgctagt ttttggaatt ctgtccattt tgatggcctt gctgtccaag
     2641 aaagtcagtc tctttgtttt tggactgatt tgctgtcttt ctctttggat taacttattt
     2701 atcacatttg ccattttgcc aatttttggc aattaaacag tcataaaagt cggagaggtt
     2761 agcttgaaaa ctaacctctt tttccttttc aaaatgggga ttcttccttg aaaataatca
     2821 gtaattgtgc taaaattaaa ggaacattct aaaatattcg gaatttaaag taaggaaaaa
     2881 catggctaat attttaaaaa caattatcga aaatgataaa ggagaaatcc gtcgtctgga
     2941 aaagatggct gacaaggttt tcaaatacga agaccaaatg gctgctttga ctgacgacca
     3001 actaaaagca aaaacagttg aatttaagga acgttatcaa aatggagaat cactggattc
     3061 attgctttac gaagcatttg cggttgtccg tgaaggtgcc aaacgtgtcc taggtctctt
     3121 cccttataag gttcaggtca tgggggggat tgttcttcac catggtgacg tgccagagat
     3181 gcgtacaggg gaagggaaaa ccttgactgc gaccatgccg gtatacctca atgccctttc
     3241 aggtaaaggg gttcacgtag ttacggttaa tgaatacctg tcagaacgtg acgcgactga
     3301 gatgggtgaa ttgtactctt ggcttggttt gtcagtaggg attaacttgg ctaccaaatc
     3361 tccaatggag aaaaaagaag cctatgagtg tgatattact tactcaacta actcagaaat
     3421 cggatttgac taccttcgtg acaacatggt cgttcgcgct gaaaacatgg tacaacgtcc
     3481 gcttaactat gccttggtcg atgaggttga ctctatcttg attgacgagg ctcgtacacc
     3541 tttgattgta tcaggtgcca atgcggttga aaccagtcag ttgtatcaca tggcagacca
     3601 ctatgtaaaa tctttgaaca aagatgacta catcatcgat gtgcagtcta agactattgg
     3661 tttgtctgat tcagggattg acagggctga aagctacttc aaacttgaaa acctctatga
     3721 catcgaaaac gtggctttga cccactttat cgataacgcc cttcgtgcca actacatcat
     3781 gcttctcgat attgactatg tggtgagcga agagcaagaa atcttgattg tcgaccaatt
     3841 tacaggtcgt accatggaag gtcgtcgtta ttctgatgga ttgcaccaag ctattgaagc
     3901 caaagaaggt gtgccaatcc aggatgaaac caagacatct gcctcaatca cgtaccaaaa
     3961 ccttttccgt atgtacaaaa aattgtctgg tatgacgggt acaggtaaga ctgaggaaga
     4021 agaatttcgt gaaatctaca acattcgtgt tattccaatc ccaacaaacc gtcctgttca
     4081 acgtattgac cactcagacc ttctttatgc aagtatcgaa tctaagttta aagcggttgt
     4141 cgaagacgtt aaggctcgtt accaaaaggg tcaacctgtc ttggttggta cagtagcggt
     4201 tgaaactagt gactacattt ctaagaaatt ggttgcagct ggtgttcctc acgaagtctt
     4261 gaatgccaaa aaccactata gagaagccca aatcatcatg aatgctggtc aacgtggtgc
     4321 cgttaccatc gcaaccaaca tggcgggtcg tggtaccgac atcaagcttg gtgaaggtgt
     4381 tcgtgaactt ggaggacttt gtgttattgg tacagaacgt catgaaagtc gtcgtatcga
     4441 taaccagctt cgtggacgtt caggtcgtca aggagatcca ggtgagtcac aattctacct
     4501 atctcttgaa gatgatttga tgaaacgttt tggttctgaa cgcttgaagg gaatctttga
     4561 acgcttgaac atgtctgaag aggccattga gtctcgcatg ttgacgcgtc aggttgaagc
     4621 agctcagaaa cgtgtcgaag gaaataacta cgatacccgt aaacaagtcc ttcaatacga
     4681 cgatgtcatg cgtgaacaac gtgagattat ctatactcaa cgttatgatg tcattactgc
     4741 agaccgtgac ttggcacctg aaattcaggc aatgatcaaa cgcacgattg gtcgtgtcgt
     4801 tgacggtcat gcgcgtgcta aacaagatga gaaactagag gcaattttga acttcgctaa
     4861 gtacaacttg cttcctgaag attctattac gatggaagac ttgtcaggct tgtctgataa
     4921 ggccatcaag gaagagcttt tccaacgtgc cttgaaggtt tacgatagtc aggtttcaaa
     4981 actacgcgat gaagaagcag ttaaagaatt ccaaaaagtt ttgattctac gagtggtgga
     5041 taacaagtgg acagatcata tcgatgccct tgatcaattg cgtaacgcgg ttggacttcg
     5101 tggctatgct cagaacaacc ctgttgttga gtatcaggca gaaggtttcc gtatgtttaa
     5161 tgatatgatt ggttcgattg agtttgatgt gacacgcttg atgatgaaag cacaaattca
     5221 tgaacaagaa agaccacagg cagaacgtca tatcagtaca acagcgactc gcaatatcgc
     5281 tgctcaccaa gcaagtatgc cagaagattt ggatttgagc cagattggac gcaatgaact
     5341 ttgcccatgt ggttctggta agaaatttaa aaactgtcac ggtaaaagac aataaaatga
     5401 gatagtttag aggcggatat cttgtgaaaa gtaaattttt actgggtatc cgtttgcttt
     5461 ataaggagat gagttatggt atttacagca aaaagctcta aaataaatat agaagaagtt
     5521 cgtgccttgt caaaattaga aggtcaggct ttggagagga aatcacagcg agatcaagag
     5581 ctagaagcca ttatacgtgg agaagaccag cgaattctct tggtaatcgg gccatgctca
     5641 tctgacaacg aagaagctgt ccttgaatac gctaagcgtt tggcagtcct acaagaagaa
     5701 gtggcagatc gtatctttat ggttatgcgt gtttatactg ccaaaccccg taccaacgga
     5761 gatggctata agggcttgat tcaccagcct aacgcgacag aagcgcctag tcttatcaat
     5821 ggaatcaaag ccgttcgcca tcttcactat cgtgtcatca cagaaacagg gatgacaact
     5881 gctgatgaaa tgctttatcc tgaaaacctt ccgcttgtag atgatttgat ttcttacatg
     5941 gcagttggtg cccgttcagt tgaagaccag caacaccgct ttgtggcaag tggggcagga
     6001 ttttctactg gttttaaaaa tccaacctct ggaaatctca atgtcatgtt taatgggatt
     6061 tatgctgctc aaaacaaaca aagtttcctt ttcttaggaa aagaagtaga aacaactggg
     6121 aacccgcttt cacacgctat tcttcgtggt gctcttaatg agtatggaaa aaatattccc
     6181 aactactatt atgacaattt aattgatacc attgcccagt atgagaaaat gggcttggaa
     6241 aatcctttta tcatcattga taccaatcat gacaattctg gtaagcagta tattgaacag
     6301 atccgaattg tccgccagac cttgattaac cgtgcttgga atgaaaaaat taagcagttc
     6361 gttcgtggtt ttatgattga gtcttatctg gaagatggtc gacaaaatga gccagaagta
     6421 tttggtaagt ctatcacaga cccttgcctg ggttgggata acacagaagc tcttgtcaga
     6481 gaaatttaca aaacgttagg agaataagat ggcatttatt gaaaaaggtc aagaaatcga
     6541 tatggaagtc atcaaggctg aaacccaatt gtctgcagaa gccttgagac tcaaggaaag
     6601 ccgtgacagg gaattggcag atattatttc aggggaagat gaccgtattc tcttggtgat
     6661 tggtccttgc tcttctgata atgaagaggc ggtcttggaa tatgctcgcc gtttatccgc
     6721 cttgcaaaag aaggtagcgg acaagatttt catggtcatg cgcgtgtata ctgctaagcc
     6781 tcgtaccaat ggagacggct ataaaggatt ggttcaccag ccagatactt ctaaggctcc
     6841 aagcctgatt aacggcttgc aggctgtgcg ccagttgcac taccgcgtga ttacagagac
     6901 tggtttgaca acggcagatg agatgcttta tccgtcaaat ctgatcttgg tggatgactt
     6961 ggtcagctac catgccgttg gagctcgttc tgtggaagac caagagcacc gctttgtggc
     7021 ttctgggatt gatgcaccag tagggatgaa aaatccaacc tcaggaaatt tgggtgttat
     7081 gtttaacgcc atctatgctg ctcaaaacaa gcaaaccttc ctttatcatg ggcaggaagt
     7141 tgagacatca ggtaatcctt tggcccatgt tatcctccgt ggagcagtca acgagtatgg
     7201 caattatatg ccgaattact actatgaaaa cctactccaa gccattgaac gatatgaaac
     7261 catgggactt gaaaatcctt ttatcctcat tgacaccaac catgataact caggcaagca
     7321 atatatggag cagattcgaa ttgttcgcca gaccttgcag aatcgtgatt ggaatgagaa
     7381 aattaaaaag acggttcgag gatttatgat tgaatcttac ctagcagatg gtcgtcaaaa
     7441 ccaaccagag atctttggtt gctctattac tgacccttgc ctaggttggg aaaatacaga
     7501 ggccttggta gaagagattt atgttacctt gacaaaataa gtgaaaagga tggagttggg
     7561 gaatctcaac tccttttgat gagaatgata gttggacacg gaattgacat cgaagaattg
     7621 gcttcgatag aaagcgcagt tacacgacat gaaggatttg ctaagcgtgt actgaccgct
     7681 caggaaatgg agcgcttcac cagtctcaaa ggacgcaggc aaatagaata tttagctggt
     7741 cgctggtcgg ctaaggaggc cttttccaag gctatgggaa cgggcattag caagctcggt
     7801 tttcaggatt tggaagtctt gaacaatgaa cgtggggcgc cttattttag tcaggcacca
     7861 ttttcaggaa agatttggct gtctatcagc cacaccgatc agtttgtgac agccagtgtc
     7921 attttggagg aaaatcatga aagctagtcc acatagacca accaaggctc tgattcatct
     7981 gggagctatt cgacaaaata ttcagcaaat gggggctcat atccctcaag gaacgctcaa
     8041 gttggctgtg gtcaaggcca atgcttatgg tcatggagct gttgccgttg ccaaggcaat
     8101 tcaagatgat gttgatggct tttgcgtttc caatatcgat gaagccattg aactcagaca
     8161 agctggactc agcaagccaa tcctcatttt aggagtttct gaaatcgaag ctgttgctct
     8221 agctaaagaa tatgacttca ccttgacagt ggctggactg gagtggattc aagcactctt
     8281 agataaggaa gtggacctaa ctggattgac agtccacctc aagattgatt cagggatggg
     8341 acggattggt tttagagagg caagtgaggt tgagcaggct caagatttgc tccaacaaca
     8401 cggtgtttgt gttgaaggaa tctttaccca ctttgctact gctgatgagg aatcagatga
     8461 ctattttaat gcccagttag aacggtttaa aactatttta gctagtatga aggaagttcc
     8521 agagctggtt catgctagca attctgcaac gactctttgg catgtagaga ctattttcaa
     8581 tgcggttcgt atgggagatg ccatgtatgg cctcaatcca agtggagcgg tcttggattt
     8641 gccttatgat ttgataccgg ccttgacctt ggagtctgct ctggttcatg tcaagacagt
     8701 tccagctgga gcttgcatgg gctatggagc aacttatcaa gcggatagcg agcaagtcat
     8761 cgcgaccgtg ccaatcgggt atgcagatgg atggacaaga gacatgcaaa atttctctgt
     8821 cttggtagat ggccaagctt gcccaattgt cggcagggtt tcgatggacc aaatcactat
     8881 tcgattgcct aagccttacc cactcggaac caaggtaacc ttgattggct ccaatgggga
     8941 taaggaaatc actgcaactc aggtagcgac ctaccgcgta accattaact atgaggtggt
     9001 ttgcctcctc agcgaccgta ttccgagaga atattattag aaaagaaagg agtggagcat
     9061 gaatctacat caacccttgc atgtcttgcc tggtgtggga ccaaagtcag cagaaaaata
     9121 tgccaaacta ggaattgaaa acttgcaaga tctcttgctc tactttcctt tccgttatga
     9181 agacttcaaa accaagcagg tgctggagct ggaagacggt gagaaggcag ttctttctgg
     9241 tcaggtagtg actcctgcta gtgtccagta ttatggtttc aagcgcaatc gcctgcgttt
     9301 tagtctcaag cagggagagg tcgtttttgc ggtgaatttc tttaaccagc cctatctggc
     9361 tgataaaata gagttgggag caacccttgc tgtctttgga aaatgggacc gcgctaaggc
     9421 tagtctgact gggatgaagg ttctggctca ggtagaagat gacctccagc ctgtctatcg
     9481 tctggctcag ggaatcagtc aggccagtct ggttaaggtc atcaagacgg cttttgatca
     9541 gggactggac ctcttgatag aagaaaatct gccccagtct ttactagaca aatacaaact
     9601 catgtcccgt tgtcaggcag tccgagctat gcattttcca aaggatttgg cagaatacaa
     9661 gcaggctctt cgccgtatca agtttgagga actcttttat ttccaaatgc agttgcagac
     9721 gctcaagtct gaaaatagag ttcagggaag tggtctggtt ctgaattggt ctcaggaaaa
     9781 agtgacagca gttaaagtaa gtcttccttt tgccctgacc caagctcagg aaaagagttt
     9841 gcaggaaatt ttaactgata tgaagtccga ccaccacatg aatcgtctcc tacaagggga
     9901 tgtggggagt ggaaaaacgg tagtcgctgg cttggccatg tttgcggcag tgacagcagg
     9961 ttatcaggct gccctaatgg taccaacaga aatcctcgca gagcaacact ttgagagttt
    10021 acagaacctt tttcccaatt tgaaactggc tctcttgaca ggttccttga aagctgcaga
    10081 aaagagagaa gtcttggaga ccattgccaa gggtgaggct gatttgatta taggaactca
    10141 cgctctgata caagatgggg tggagtatgc tcgtcttggt ttgattatta tcgatgagca
    10201 gcaccgtttt ggtgtagggc aaaggcgtat tttacgggaa aaaggtgaca atccagatgt
    10261 cctcatgatg acggcgactc ccattccacg gacgcttgcc atcacagcct ttggagatat
    10321 ggatgtttcc attatcgacc agatgccagc aggtcggaag cctattgtga cgcgctggat
    10381 caaacatgag caactacctc aggtcttgac ttggttagag ggggaaattc aaaaaggttc
    10441 ccaagcctat gtcatctctc ctttgattga agaatcagaa gctctagatt tgaaaaatgc
    10501 cattgcctta tcagaggagt tgacgactca ttttgcaggc aaggcagagg tggctcttct
    10561 acatggtagg atgaagagtg acgaaaaaga ccagatcatg caggatttca aggagagaaa
    10621 gacggatatt ctggtttcga cgacggttat tgaggttggg gtcaacgttc ccaatgcgac
    10681 tgtcatgatt atcatggatg ccgatcgctt cggtctcagt caacttcacc agcttagagg
    10741 tcgtgtcggt cggggggaca agcagtccta cgctgttctc gttgccaatc ccaagacgga
    10801 ttctgggaaa gaccgcatgc gcatcatgac agaaacgacc aatggatttg tccttgcgga
    10861 ggaagatttg aaaatgcgtg gttctggtga gatttttgga accagacagt caggacttcc
    10921 agagttccaa gtggctgata ttatcgaaga ttttccgatt ttagaagaag caagaaaggt
    10981 tgctagctac attagttcta tagaagcttg gcaagaagat ccagagtggt gcatgattgc
    11041 ccttcatctg gaaaagaaag aacatctgga ttaagctttc tctaaggaaa acttaagcta
    11101 gtttttaggt ttggctctta tactaaagtc atcaaaaaga aacgaggact ctcatatgac
    11161 agtaacgatt aaagtaaatt accaaaccac tttccaaaag aaggaagcaa aaaactagta
    11221 taaacagaag agagagcgaa atgctctttt tcgtttctaa aactactttc agcccatcat
    11281 cctaaaagta aagaatctaa attcactttc tatttatcct tctttcttgc attgattaca
    11341 tagatatgct acagttgtgg taacgattac aaaataaaag gagcatgcta tgaaaaatcc
    11401 agctttgcta gaagaaatta agacctatag aggaagggat gaggttccgg aagactttga
    11461 tgctttctgg gatggggaag tgaaaaatgt ttccacgctt ccatcctacc acttggagga
    11521 aagagatttc cacattcctc aagtcaagtg ctatgagtta acatttgaag gaagcaagga
    11581 aggaaaggtc tatgcacgca ttgttcttcc aaagagtgag gagaaggttc cactaatctt
    11641 ccattttcat ggttatatgg gacgtggctg ggactgggcc gacatgctgg gcttcaccgt
    11701 agctggttac ggtgttgttt ccatggatgt gcggggccag tcaggttact cacaagacgg
    11761 cttgcgttct cctttaggaa ataccgtgaa ggggcatatt atccgtggtg ctatggaagg
    11821 tcgggaccac ctcttttata aggatgttta tctggatatt taccagttgg tcgaaattgt
    11881 tgctagtctg tctcaggttg atgagaagcg tctttctagc tatggtgcct cacaaggagg
    11941 ggctctagct ctagttgcag cagcgctcaa tcctcgaatt cagaaaacag ttgccattta
    12001 tcccttcttg tcagacttca gacgggtgat tgagattggt aatactagcg aggcttacga
    12061 cgaacttttc cgttatttca agtttcacga ccccttccat gaaacagagg aggaaatcat
    12121 ggcgaccctt gcctatatcg atgtcaaaaa tcttgcccat cgtatccaag gtgaggttaa
    12181 gatgattacg ggcttggacg acgatgtttg ctatcccatt acccagtttg cgatttataa
    12241 tcgtctgacc tgcgataaaa cctatcgcat catgcctgag tatgctcacg aagccatgaa
    12301 tgtatttgtc aatgaccaag tctacaactg gctctgtgga agtgagattc cttttaaata
    12361 tctaaaataa ggagtcgact ctaagcacaa aatcttaaaa attacaaaca cgcatagtat
    12421 caggggatta aaaaaacttg atactatgcg ttttatcatg gaaatatagt aaaatgaaac
    12481 aagaatagga caaaacgatc aggacagtca aatcgatttc taacaatgtt ttagaagcaa
    12541 atgtgtacta ttctagtttc aatctattat atttatagaa ttttttgttg ctagatttgt
    12601 caaattgctt aaaataattt ttttcagaaa gcaaaagccg atacctatcg agtagggtag
    12661 ttcttgctat cgtcaggctt gtctgtaggt gttaatactt ttcaaaaatc tctttaaacc
    12721 acgtcagctt cgcctcgccg tatatatgtt actgacttcg tcagttctat ctgccacctc
    12781 aaaacggtgt tttgagctga cttcgtcagt tctatctgcc acctcaaaac ggtgttttga
    12841 gctgacttcg tcagttctat ctgccacctc aaaacggtgt ttgagctgac ttcgtcagtt
    12901 ctatctgcca cctcaaaacg gtgttttgag catcatgcgg ctagcttctt agtttgctct
    12961 ttgattttca ttgagtataa aaacagatga gtttctgttt tctttttatg gactataaat
    13021 gttcagctga aactactttc aaggacatta ttatataaaa gaattttttg aaactaaaat
    13081 ctactatatt acactatatt gaaagcgttt taaaaatgag gtataataaa tttattaaca
    13141 cttataaaaa gtgatagaat ctatctttat gtatatttaa agatagattg ctgtaaaaat
    13201 agtagtagct atgcgaaata acagatagag agaagggatt gaagcttaga aaaggggaat
    13261 aatatgatat ttaaggcatt caagacaaaa aagcagagaa aaagacaagt tgaactactt
    13321 ttgacagttt ttttcgacag ttttctgatt gatttatttc ttcacttatt tgggattgtc
    13381 ccctttaagc tggataagat tctgattgtg agcttgatta tatttcccat tatttctaca
    13441 agtatttatg cttatgaaaa gctatttgaa aaagtgttcg ataaggattg agcaggaagt
    13501 atggtgtaaa tagcataagc tgatgtccat catttgctta taaagagata ttttagttta
    13561 attgcagtgg tgtcctggta gataaactag attggcagga gtctgattgg agaaaggaga
    13621 ggggaaaatt ggcaccaatt tgagatagtt tgtttagttc atttttgtca tttaaatgaa
    13681 ctgtagtaaa agaaagttaa taaaagacaa actaagtgca ttttctggag taaatgtctt
    13741 atttcagaaa tcgggatata gatatagaga ggatcagtat gaatcggagt gttcaagaac
    13801 gtaagtgtcg ttatagcatt aggaaactat cggtaggagc ggtttctatg attgtaggag
    13861 cagtggtatt tggaacgtct cctgttttag ctcaagaagg ggcaagtgag caacctctgg
    13921 caaatgaaac tcaactttcg ggggggagct caaccctaac tgatacagaa aagagccagc
    13981 cttctgagga aggagaaaat ataactcttc ctgcagagca tgtagagtct gtttcagaga
    14041 ctgaactttc tggcaataag caagaacaag aaaggaaaga taagcaagaa gaaaaaattc
    14101 caagagatta ctatgcacga gatttggaaa atgtcgaaac agtgatagaa aaagaagatg
    14161 ttgaaaccaa tgcttcaaat ggtcagagag ttgatttatc aagtgaacta gataaactaa
    14221 agaaacttga aaacgcaaca gttcacatgg agtttaagcc agatgccaag gccccagcat
    14281 tctataatct cttttctgtg tcaagtgcta ctaaaaaaga tgagtacttc actatggcag
    14341 tttacaataa tactgctact ctagaggggc gtggttcgga tgggcaacag ttttacggta
    14401 attacaacga tgcaccctta aaagttaaac caggtcagtg gaattctgtg actttcacag
    14461 ttgaaaaacc gacagcagaa ctacctaaag gccgagtgcg cctctacgta aacggggtat
    14521 tatctcgaac aagtctgaaa tctggcaatt tcattaaaga tatgccagat gtaacgcatg
    14581 tgcaaatcgg agcaaccaag cgtgccaaca atacggtttg ggggtcaaat ctacagattc
    14641 ggaatctcac tgtgtataat cgtgctttaa caccagaaga ggtacaaaaa cgtagtcaac
    14701 tttttaaacg ctcagattta gaaaaaaaac tacctgaagg agcggtttta acagagaaaa
    14761 cggacatatt cgaaagcggg cgtaacggta aaccaaataa agatggaatc aagagttatc
    14821 gtattccagc acttctcaag acagataaag gaactttgat cgcaggtgca gatgaacgcc
    14881 gtctccattc gagtgactgg ggtgatatcg gtatggtcat cagacgtagt gaagataatg
    14941 gaaagacatg gggagataag gtggttatct ccaatcttcg agataatcct gaagctaaag
    15001 atcctgctgc gccatcgcct ctaaatattg atatggtttt ggttcaagac ccgacaacaa
    15061 agagaatctt ctcaatttat gatatgttcc cagaaggtcg agcagttttt ggaatgccaa
    15121 aaacacctga aaaagcttat gaaaagatag gggataaaac ttatcaaatc ttgtataaac
    15181 aaggagagtc tggtcattat actgttcgtg agaatggaga agtgtataat gcacaaaatc
    15241 aaaagacgga ttatcgtgtt gtagtgaatc caacagaacc tggctataga gataaaggaa
    15301 atctttacaa aggtcaggaa ttgattggaa atatctattt tgcacacagt acaaaaaatc
    15361 catttagagt agccaatacg agctatctat ggatgtcata tagtgacgat gatggtaaaa
    15421 cttggtctgc accgagagat attactccag gtcttcgcaa ggattggatg aagttcctag
    15481 gaacaggtcc tggaacagga attgtacttc ggaatgggcc tcacaaggga cggattttga
    15541 taccggttta tacgactaat aatgtatctc acttagatgg ctcgcaatct tctcgtgtca
    15601 tctattcaga tgatcatgga aaaacttggc atgctggaga agcggtcaac gataaccgtc
    15661 aggtagacgg tcaaaagatc cactcttcta cgatgaacaa tagacgtgcg caaaatacag
    15721 aatcaacggt ggtacaacta aacaatggag atgttaaact ctttatgcgt ggtttgactg
    15781 gagatcttca ggttgctaca agtaaagacg gaggagtgac ttgggagaag gatatcaaac
    15841 gttatccaca ggttaaagat gtctatgttc aaatgtctgc tatccatacg atgcacgaag
    15901 gaaaagaata cattatcctc agcaatgcag gtggaccgaa acgtgaaaat gggatggtcc
    15961 acttggcacg tgtcgaagaa aatggtgagt tgacttggct caaacacaat ccaattcaaa
    16021 aaggagagtt tgcctataat tcgctccaag aattaggaaa tggggagtat ggtatcttgt
    16081 atgaacatac tgaaaaagga caaaatgcct ataccctatc atttagaaaa tttaattggg
    16141 actttttgag caaaaatctg atttctccta ccgaagcgaa agtgaagcga actagagaga
    16201 tgggcaaagg agagatgggc aaaggagtta ttggcttgga gttcgactca gaagtattgg
    16261 tcaacaaggc tccaaccctt caattggcaa atggtaaaac agcgactttc ctaacccagt
    16321 atgatagcaa gaccttgttg tttgcagtag ataaggaaga tatcggacag gaaattattg
    16381 gtatagctaa aggaagcatc gaaagtatgc ataatcttcc tgtaaatcta gcaggtgcca
    16441 gagttcctgg cggagtaaat ggtagcaaag cagcggtgca tgaagttcca gaatttacag
    16501 ggggagttaa tggtatagag ccagctgttc atgaaatcgc agagtataag ggatctgatt
    16561 cgcttgtaac tcttactaca aaagaagatt atacttacaa agctcctctt gctcagcagg
    16621 cacttcctga aacaggaaac aaggagagtg acctcctagc ttcactagga ctaacagctt
    16681 tcttccttgg tctgtttacg ctagggaaaa agagagaaca ataagagaag aattctaaac
    16741 atttgatttt gtaaaaatag aaggagatag caggttttta agcctgctat ctttttttga
    16801 tgacattcag gctgatacga aatcataaga ggtctgaaac tactttcaga gtagtctgtt
    16861 ctataaaata tagtagattg aaataagatg tgaacaactc t
//
LOCUS       DAVLWA010000035        16753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLWA010000035 DAVLWA010000000
VERSION     DAVLWA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.035"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..691)
                     /locus_tag="VYN80_001142"
     CDS             complement(<1..691)
                     /locus_tag="VYN80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1541061.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKI"
     gene            complement(1136..1535)
                     /locus_tag="VYN80_001143"
                     /pseudo
     CDS             complement(1136..1535)
                     /locus_tag="VYN80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2043..2525)
                     /gene="greA"
                     /locus_tag="VYN80_001144"
     CDS             complement(2043..2525)
                     /gene="greA"
                     /locus_tag="VYN80_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1541062.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2596..4251)
                     /gene="mltG"
                     /locus_tag="VYN80_001145"
     CDS             complement(2596..4251)
                     /gene="mltG"
                     /locus_tag="VYN80_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1541063.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(4332..4826)
                     /locus_tag="VYN80_001146"
     CDS             complement(4332..4826)
                     /locus_tag="VYN80_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541064.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(4823..5287)
                     /locus_tag="VYN80_001147"
     CDS             complement(4823..5287)
                     /locus_tag="VYN80_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541065.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(5297..6631)
                     /locus_tag="VYN80_001148"
     CDS             complement(5297..6631)
                     /locus_tag="VYN80_001148"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1541066.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSTREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6642..7259)
                     /locus_tag="VYN80_001149"
     CDS             complement(6642..7259)
                     /locus_tag="VYN80_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1541067.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(7307..10405)
                     /locus_tag="VYN80_001150"
     CDS             complement(7307..10405)
                     /locus_tag="VYN80_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1541068.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10498..11664)
                     /locus_tag="VYN80_001151"
     CDS             complement(10498..11664)
                     /locus_tag="VYN80_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1541069.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            complement(11672..12766)
                     /locus_tag="VYN80_001152"
     CDS             complement(11672..12766)
                     /locus_tag="VYN80_001152"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1541070.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(12924..14366)
                     /locus_tag="VYN80_001153"
     CDS             complement(12924..14366)
                     /locus_tag="VYN80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541071.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(14372..14605)
                     /locus_tag="VYN80_001154"
     CDS             complement(14372..14605)
                     /locus_tag="VYN80_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV1541072.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            complement(14777..16735)
                     /locus_tag="VYN80_001155"
     CDS             complement(14777..16735)
                     /locus_tag="VYN80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1541073.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT         4765 a         3690 c         3072 g         5226 t
ORIGIN      
        1 gaatcttgtg ttgttttaag atgagtttta gagcctgatt gaccacctca gcacttttat
       61 ttggaatcaa tcggatgatc tgatgtctac tctttcgatc cgtcaagaca agcaagcagt
      121 agtttttcgc tctcgtaagt agaaccgtat caatctcata atgcccattc tccaagcgaa
      181 gattgatagc ttcagaccgc tgttcgatgg attgaccagc aggtttaaag ttggtgctag
      241 cctgtttctt aagcgctttt ccttttctag ggtaaagcag atcctgtttg cttaacccca
      301 attttccatg atgaatccaa tagtaaatgg ttgaaattcc cacgttaacc cctttagcca
      361 tcaccatcat ttcaggcgaa aatttttggt tatgatagtg gagaatcttt tcctttagtt
      421 ccttggtcaa gcttgatttc ttgaccgagc gcttgcgatt attttcataa gactgttgag
      481 catagtcggc agaataaacc tctttgaagc gcccttttcc aagacattgt cggactgtcc
      541 cacgcttgat ttcagtgtgg atagtttgag gaacttttcc aagcagagag gcaatttctc
      601 tatttgattt cccttctttt ttccatcttt cgattaagcg acggctatcg attgtcaaat
      661 gtttggcttt tgtagtataa ttgtcttgca tctctgtgcc tttcttgtgt ttgtggttga
      721 acaacaagta taacacagag gtgttttctt atgcctacaa gagctttcat tatttccatt
      781 ttcttttgga tttcactcta ttctgaaaaa cttgtgtata tttactgaag ctagcaagtc
      841 ttacctgtaa atttaatgaa agcaacacaa aatccgagag gggaatctcg gattaataga
      901 tagagagttt ttagtttaaa taaattgttt aaaatatcaa caacatcact tcttttctta
      961 acctgataag tcttgattcc taattttggg gctacgatta tattgtcctc aatatcgtct
     1021 agaaagacac aatttctagg ttataactgg tatttatcga tagttactca tatacatcag
     1081 tccacctcca tacttatgtg cgagcctctc tttgtattat acctccatac tcaccttaca
     1141 gattcttttg gtaataatat ctttgcctaa tgtagagata gtcttgcaaa gaaaaaactt
     1201 ccttgtagcc atgtttctga taaaagtccg gtgcctggaa ctggtaagta ttgacaaagg
     1261 caaaacaaca atttcgattc ttagcttcac tttctgcctg ttgcaatagt tttgaaccga
     1321 ttccttgccc tcgcagttcc tcttttacaa acaaatactc gatttctagc caatttccaa
     1381 aagtctctgc tatcaaacct gccaggagat tgcccttttc atcttcgaca taaagaatta
     1441 agtggctcac tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct
     1501 atttcttgcg atttatgtga ttccttattt tccaatctaa agtatagtga aatgaaataa
     1561 aacatgcgca aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa
     1621 gtgtactatt ttaacttcaa tgcactatac atctaatact caataaaaat caaagagcaa
     1681 actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga tagaactgac
     1741 gaagtcagct caaagcatgg ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaag
     1801 catggttttg aggttgtaga tgaaactgac gaagtcagct caaagcatgg ttttgaggtt
     1861 gtagatgaaa ctgacgaagt cggctcaaaa catggttttg aggttgtaga tgaaactgac
     1921 gaagtcagta accatacata cggcaaggcg acgctgacgt ggtttgaaga gattttcgaa
     1981 gaatagcaaa atggaaaaag gagtgagtga agcacatcgc ctccccactc ctttttctgt
     2041 ttttaggctg ttttttcaac cttcaagatt tttacatcat agctaccaac aggcgtttca
     2101 atggttgctg tatcacctgt tttcttgcca atcaaggcct gcccaattgg gctttcattt
     2161 gaaaccttac ctgcaaaggc atccgcacca gctgaaccta cgataatata aacttcttct
     2221 tcgtcctcac caatttcttg gatggtgact gttttaccaa tcgctacttc gtcctgggca
     2281 actgcgtcgc tattgacgat ttcagcatag cggatttttg tttctaagct agagatttgt
     2341 ccttcgacaa aggcttgttc atccttagct gcttcgtact cactgttttc tgaaaggtca
     2401 ccgtatgaac gggcaatctt aatgcgttct accacttctg gtcgacgaac caatttcaat
     2461 tcttctaatt ctttttcaag tttttccttt tcctcaaggg tcataggata tgttttttct
     2521 gccatttttc tcaactttct tctgataata ttttctaaag aaaattatgt gaagtataac
     2581 ataattttag tttgtttagt ttaatttgct gttgacatgt tcagcgacat tgcggtcgtg
     2641 gtcttcttga ttgttagcat agtagacctt gccttctgtg acatctgcta caaagtagag
     2701 gttatcgctc ttagtttgat tgatgcttga ctcaatcgca tccagacttg gactatcgac
     2761 tggaccaggc atgagaccta catttttata aacattataa ggtgaatcaa tgttggtatc
     2821 aatcgcaaca tcctcagcta gactgatatt ttgccccagt tttccttggg catacaagat
     2881 tgcaatatta ctttgaagtg gcatatcacg attcaaacga ttgtagaata cacctgcaat
     2941 gagcttacga tcttctgtct tggcaccttc tttttcgacc aaggaagcaa tggtcaacaa
     3001 ctcattgaca gtcaagtttt tagatttgat agtactatag taaggagata ggttcttatc
     3061 catagcagct aacatctcat caatcaagct ctcaatagtt gtgctttcct tgatagagta
     3121 tgtagctggg aaaaggtatc cttccaaacg ataacgcgca ccgctgtctt ttacaggcaa
     3181 actttccagt aaagtaggat atttcgctac tgcttgactg ataaacgtct catcttgaac
     3241 tttagccaag aaagtctccg ctgtcaaaga ctctttgaag tcaccttgca attgacccac
     3301 agcttgagca atctgatcca aggtataacc ttctggaatt gtcaaagtcg caagtacagg
     3361 ttcttgcggt tcatctgttc cacctttttg caactctttg agtaagtctt ctgtactcat
     3421 actcttttgc aaattgtagt aacctgcttt caagtcggta taatttttat acttggcata
     3481 aaaactaaaa atcagaccat gctttaccaa accagctttt tcaagcgtcg taccgatttc
     3541 ttgaacgttt gaaccttctg gaattccaac cgtcacatat ttcttagaat tagcatcgat
     3601 aggtaataag gaatctagca cgtactggta accaaagtaa ccacctgctg agagcaaggc
     3661 aaggaataca aggaatgaaa tcacaaaagc tttcacatgt gacttcttct ctttcttagg
     3721 cttaacgggt tttgctcctt ctctacggct acgacgtgat gtatctctga tgatatccac
     3781 tgtttctgta gcaggcgtag gtgtttctgg acctgcttgt tctgcttttt tctctgccgt
     3841 cgtataggaa acagctaccc ttgttggggt ttcattgtat tctctttcaa gtttcttagg
     3901 tctaacagga cctggacctg gtcttgatcc actttcttcc gctggagaag aaggtacatc
     3961 ttgacttgga tgacttggaa caccaggagt ttctctttga atctcatctg ctggagaagc
     4021 tggtacacct tgacttgggt gagtaggcac ggtaggagct tttctcataa tctcctctac
     4081 cgttgacaag gaatcagcca tgagttcttc agttgaaggt tcatttgcag gagtgcgaac
     4141 tactgcctca tcttctttca gaacttcatc atagcctttt actttttcta aatctctcag
     4201 aatctgctct ttaaagctta atttctcttc ttctcttgac ttttcactca aaagtttttc
     4261 ctccttgttg ataatccata atattatatc ttaaaagtcc aaaaaaagca atctatgata
     4321 cttttcctaa cttatttttt catttcccag accatatcat accatgtttc ccctgcaaag
     4381 gttgactggg actggccttc gttgacaaat ccatgttttt catagtaggc gatgagatag
     4441 tcatgacagg ttagattaat gccatctctc tcatcttcaa gagcaacttc tttcaaggct
     4501 gtcagcaatt tctgacccag tccgagtccc tgtgcctcct tggcaataga cagacaggtc
     4561 acagagatat aaccaccagg ctcatgacta tagtctttta tttcttctgt aaaagactgg
     4621 tcttgcagat ggcggtgcag gccaactggt ccttcgatat aacccatgat tcttccttct
     4681 ttttcagcaa ccagaaaaga ggtctgaatt tctctcaaat gtgcttcaaa gacagaagga
     4741 ggaatggctt cttcgaccga aaaattttca aattcaagtt caacaatccg atccaaatct
     4801 tctaatcttg cttgtctgat tttcattgtt cctccagata aaagggatta aaccaaatca
     4861 tactatagcc ctggctagtt gcatagagca aagttgcttc ttcatcaaca aaaccgttca
     4921 tttcaaaata ggaaagcagc tcatcaggac tctccaaacg aatccctttg taatccagct
     4981 caactgccac ctctttcaag gctgcaagaa gaagtgttcc caagccctgt ctctgatggt
     5041 cagactcgat gactaaagaa tgtactttta gatattgcgg attgtctgac tggggacttg
     5101 ataaaatata gcctaaaagt tgattttcat ccctagctag aagaaaggta tccgcacact
     5161 tacggatact ttcttctaaa atatgggaaa gttgctgctt ttcagctgga aaagacgagg
     5221 tctgaagtgc ccctatctca ggcaaatcag acttgcttgc ctgaatgatc ttaattggaa
     5281 tttccatggg aacatcctat tgaacattgc ttgtcaagtt agacaagaga cgctcaaatg
     5341 agtattcata ggtttggatg tctcctgctc ccataaagac gtaaacagca ttgtcatggt
     5401 ctaggagtgg agaaacattt tcaacagtaa tcacttggtg ttttttgttg attttgttgg
     5461 ctaggtcttc taccttaacg tcaccatgat ctacttcacg agtcgagcca taaatttgcg
     5521 ctagataaac agcatctgct tggtttaaag catgggcaaa gtcgtccaac aaggcaatgg
     5581 ttcttgtaaa ggtatgcggt tgaaagactg ctacaatttc cttgcttggg tatttctgac
     5641 gagccgcatc caaggtcgca ataatttctg ttggatgatg ggcaaagtca tcgataatca
     5701 ctgtatcatt gacaattttc tcagtgaaac gacgtttaac accggaaaat gttttcaagt
     5761 gctcacgcac caagttcaaa tcaaatcctg ctgtgtaaag aagaccaata acggctgtcg
     5821 cattcatgat attgtgacga ccaaaggttg gaatgtggaa ttgccccaag ttttgtccac
     5881 ggaaatgaac ggtgaaggtt gaaccagttg ttgaacgaag aagatcacta gctacaaagt
     5941 cattgccttc agcttcaaaa ccataataat aaattggtgc atcagacgta atcttacgca
     6001 attcagcatc ttcaccatag acaaaaagac ccttggtgat ttgtttggca tagtcgttaa
     6061 aggcattgaa aacatcctcg agacttgtga aataatctgg atggtcaaag tcaatgttgg
     6121 tgataataga gtattctggg tggtaaggca tgaagtgacg ctcatattcg tcagattcaa
     6181 agacaaaata tttggcattg gccgaaccac gacctgtccc atctccaatc aagaagctgg
     6241 tatctgtaat gtgagacaag acatgagaca acatacctgt cgttgaagtt tttccatgtg
     6301 ctcctgctac tcccatgcta acaaagtcac gcataaagct acctagaaac tcatggtaac
     6361 gtttgtagct aataccattt tggtccgcat aggcaatttc gacgttgtta tctggacgaa
     6421 aggcatttcc agcgataatt tccatatcac cgtctagatt cttttcatca aaaggaagaa
     6481 tggtaattcc tgcctgctca agaccgcgtt gggtaaagta gtacttttca acatctgatc
     6541 cctgaacctt gtgccccatc tggtacaaca tcaaggccaa ggcactcatc cctgatccct
     6601 taattccgat aaaatgatat gtctttgaca tgttttctcc cctattctgt cattctggtc
     6661 agattcaact cttgggcaac ccgacgttct tgttctgttt gtttactttt tttattgtag
     6721 atttggctct tctttagaaa atcataattg tttttctttg gagcaggtgc tgacacttct
     6781 tcattcttgg tagggataga atgaacttct tccgccaaga tataatgaga ctgggtcaat
     6841 ttttggctat atttgacaaa ttcaccagga ttttcctttt ggaaaggagt tgtcggttga
     6901 ttgccctgtc taactagact gggctgagaa tgacgtctcg caaggctgaa atcctgagtt
     6961 agatagttag cagagcgttt ctttttcaag tctgcacgcg cttcttcacg cgccacctcc
     7021 gcatagctct ttccttcttt tttaacccct aaaggagctt ttttaggttt ccctacttgc
     7081 ttttcaatcg gttttactgg tttttcttca gcaataggag cccattctaa ataattttta
     7141 tctcgatact cacccttgat attactgatc agatcagact catcatagag attcatgact
     7201 ggcatttcag tcaacatgac ctcgtcatct gacaccaatg gaaatcgttc ttgtttcatt
     7261 ttctatttcc tttcaacact tcattatagc gtattgtctt gatttttcaa gtgctggctt
     7321 cagaaattcc caaaatttct ctaatttctg ctagggtcag actaccacgt gactctgtgc
     7381 cgtccaatac ttgtgacacc agatgtttct tttgttcttg gagttcctga attttttctt
     7441 caatggttcc cttggtcacc aagcgataga cctcaaccgt ttcttcctga cccatccgat
     7501 gggcacggcc aatggcttgc gcttccaccg caggattcca ccaaaggtca accaagatca
     7561 ctgtatctgc acctgtcagg ttcagaccga ccccaccagc cttgagggaa atcagaaagg
     7621 catctctttc tccttggtta aaggccttgg tcatgtcttg tctttccttg gctggggttg
     7681 aacccgtaat tttaaaggaa gtcaggccca agtctggcag ttcttgttca attttttcca
     7741 acattccctt gaactgagag aaaatcaaga cacggtgtcc gccgtctgcc acctgtacca
     7801 gtaggtctcg gagactatct agtttgccgc tggctccctg ataatcttcc ataaacaggg
     7861 caggagtgtc acatatttga cgcaagcgca tcaaaccaga taaaatttcc acacgacttc
     7921 gctgaaattc ctgttctgac acttgagcca gatggtctcg catctgttgt aactgggcaa
     7981 ggtaaatagc cttttgctgg tcttccagtt catttttata aatcacctca atcaagtctg
     8041 gcaattcagt cagaacttct tctttcttgc gtcgcatcac gaaaggcttg ataaactgag
     8101 ccactcgctc tgctggcaat ttcataaatt ctttcttgct tggcaaaagt ccgggcatga
     8161 cgatttggaa aatagaccac aactcaccca gatggttttc aatcggagtt cctgacaagg
     8221 caaagaccga cggcaccaca aattgtctca aggtctgggc aatcttggtc tgggcatttt
     8281 tcatgacctg agcttcatct aagaaaagga agtcaaaggc catcccctga taaagctcac
     8341 tgtcctgacg gaaggtggca tagctagtca catagatttg atggttatcg gcaagaatct
     8401 cctcacgact tgctttcaaa ccatgaacaa cagccacatc caactgtgga gcaaatttct
     8461 gaaactcatc cgcccagttg taaatcaaac ccgacggagc gagaatcaag actcgacttt
     8521 cttttgtcac ttgactagtc aaaaaagcaa tggtctgaag ggttttccca agtcccatat
     8581 catcagccaa aatcccacca aaaccataat gatggagcat ctgcaaccag ccaattccct
     8641 tttcctgata atctcgcaag tcagtcttga cctgagttgc ttgcaaagga aagtcctctg
     8701 gatgtgtcaa atcctgggcc agattctgga attcttgtga aaaagaaaca cggtctcgcc
     8761 cttcaaagag atgagctaaa ctgtaggcta aggatttccg agcctgcaag gtcccatctt
     8821 ttaattcaaa ttgccccagt tcctgtagat tttggcgaat tttcttggtt tcttcatcga
     8881 aaaagtaaac ttgattagac gaatcaatat aaaaatcctg attggcaacc aaggcctgta
     8941 tggcttggtc gatttcctcc tgggcaatat cttgaaaatc aaactggatt tccaagagac
     9001 ctcccttgga ggcaatctgc acctgaggac tcgctaggct ataaagctct tctagtttat
     9061 ctgataggtc aacatgcccg agtttttcaa agactggaat gatatcatga aaaaaatgat
     9121 agacagactc cgcttttaag gcctgacgcc aagattgaaa atcggcttca aagcccgcag
     9181 ccaaacagac ttggaaaatt ctttcttcta agtccgcgtc acttgaaaat ggtaattctt
     9241 ctagctcttg tcggctagat acctgtctat ttccataatc aaactgaatt tctaaacgaa
     9301 tccgattatc ttcttccctg tcaaagtaaa aagagggcgc aaaagttttg atttgtagac
     9361 gttctggagc tgaaacggtg cccatctgga taaaaagagt cagacaggag gccaatttgt
     9421 ctcgatcact gctatcaaat tgcaggtatt tctttccttg tcgatccaca ggtaacgctt
     9481 taatttcctt gagaagacgc atctgctggt ctgttaaaaa ataaacctga cctttatgga
     9541 aaagtactgc tccttgataa aagacattga ccctaggact ctcactgatt tccatttcaa
     9601 aatagtccga atattctgtt actgtaaagg caaatagatt ggcatcagca tgcatatcct
     9661 gaaaaagcag ggtttggtag ctatccactt gatggtcaaa ttgaaaatgg ggcaaggcca
     9721 tcagtaaatt cacaccctgc tcaaaaaagg tcagagggaa aaagaggtgc cgaccttggt
     9781 tttggaaaaa gaggtctgga accagccctt cctccgttag tccgtgcaag aaagtcaaaa
     9841 gttcttggct ggcatcatca aaggcttccc aagaaagaga ctcctcataa atcttgccaa
     9901 tcatatacga ctttctctgc tcgacaatcc ttaaaaaaag tggaatatcc cgaatgacat
     9961 agtatttttg gctattgatt tggccgattc tcagagtcca caagatatga ttggttcctg
    10021 cttccacctg acccacagct gataactcat aggcgcattc tgattttgga gataaaattc
    10081 gatccaaaaa cttgccaccc aaggtcacct tggtttcaac ggcctctttt tcttcatgac
    10141 cttcttccag actccacaag atttcctgac cacgctcatc atttttcaga aaatgctcta
    10201 gcgctgccaa atgcacacag tagcccctct tttgaaaaaa atcacaggca caaaaaacca
    10261 aatcatcctc taaactatag cgcagttctt cttctgcaac gcgagcgtag agccgattgt
    10321 tcttttcctt gatgatatca accttaccag tttcataaag ggcaacacct tcgatacgaa
    10381 ttttccccgg aatcaattta gccatatttt cacctttacc ttatcttttt attataccat
    10441 attttcgcct atgaaaatag ccttctagga agacttttct cctagaaggc tggattttta
    10501 acatttggca aaagtagcca caatccgctg acagacttct tgcaacagag atttgggcat
    10561 agctatattg atgcgggcat ggagacttcc ttcctctcca aaatccaaac cacggttgag
    10621 gataaccttg gcttcatttc tcaacaactc ttgcaatgtt tcatcagtca ggtcataggc
    10681 tgaaaagtca agccaaatca agtaggtacc ttgcggtttc atgaccttga ttttagtctc
    10741 ttttccaaat agatccacca cataattgat gtggtcttca aagacttgct tgagttcctc
    10801 tagccaatct ttaccgtatc gataggcagc ttctgtcgcc aaataaccca agcctgaaat
    10861 ttcatgctga ttattggcca acaggcgttt ctggaaagcc agtctcaact taggattttc
    10921 aatgactgca taggaatttt ttgttccagc aatattaaat gttttagtgg cactgctcaa
    10981 gacgatagca aaatttttga aggcaggatt gatggtattg aaagactggt gtttgtgacc
    11041 aaagagggtc aaatcttggt gaatctcatc cgaaactaac aaaacaccgt gtttttggca
    11101 gagttggcca atcttctcca acacttcttt ttcccaaaca cgtccaccag gattgtgagg
    11161 gttgcaaaga acatagagtt taacctcctc ttccaccaaa tccttttcaa gttggtcaaa
    11221 gtcaatctca aacagactat ccttttccac taaggaatta gtaatcaatc tacgattatt
    11281 caacttgaca ctgcgagcaa agggtgggta gacaggcgtg ttaattaaaa ccgcctcgcc
    11341 ttcttttgta aaggtttgaa tagctgttga gatggctggt accacaccct cgataaagac
    11401 aagagcctct ttgtcaaagt tgtaaccgta ttgtgtagct tcccactttt gaacttcctt
    11461 aattaagtct tcactggcat aggtataacc ataaaccagt tggtctgcgt aagtttgcac
    11521 ggcttggcgg atttcaggca agaccacaaa gtccatatcc gctatccaag ctggtagaac
    11581 ttcactatcc gtttctgttt ctttccattt ataggtatgg tgccctaaac ggttgggcag
    11641 gcttgtaaaa tcatattttc ccatctttgt cttatccttc taaggcttgg cgcaaatctg
    11701 caatcaaatc tctagcatcc tcaatcccaa tagacaaacg caagaggtca tctgtcaaac
    11761 cataagaatg gcgtacttct gctggaatat cagcatgagt ttgagtcgtt ggataagtaa
    11821 taagactttc cactccgccc aaactttccg caaaagagaa gaccttgaga ctgttcaaaa
    11881 tatgaggaat gcgtgtttca tcggctactt taaaggaaat catgcctcca cgaccagtgt
    11941 agagaacttc cttaactgct ggagaatcct tcaaaaaggc aaccacttct tgggcgttag
    12001 ctgttgagcg ctccatacga agagacaagg tcttgagacc acgaagcaac tggtagctgt
    12061 caaatggaga caagactgcc cctgttgtat taagattgta aaaaagcttc tcgtatagtt
    12121 ctaaactatt ggtcacaacc actccagcca agacatcatt gtggcctgct agatacttgg
    12181 ttgctgaatg gagaacgata tctgctccat cttcaatcgg acgttggtaa atagggctat
    12241 agaaggtatt gtccaccacc actttggaac ccttagcatg agccaatttt gctagttttt
    12301 cgatatcaaa ttccaacatc aagggattgg ttggggtttc gatatagaga acatccacat
    12361 ccttttctaa ctcggcaatc aactcttctt ctgtattggc ataggtaaaa tggaaatgac
    12421 cttcctgctc cacttggtta aaccagcgaa aagaaccacc gtaaagatca cgcactgcca
    12481 agaccttact tcctactgga aagacgctaa aggccagtac aatagctgac atccctgagc
    12541 tagtcgctag ggcatagtct gctgactcaa tagccgccaa gacttcctca gccttactac
    12601 gagttggatt tttagtgcgc gtatagtcaa acccagtaga tcgaccaaac tctggatgct
    12661 gataggtcgt tgaaaaatga agtggtgtca ccaaagcacc tgttgcctca tcagacttga
    12721 tccctgcttg tgctaaaatt gtgttaatgt gtaattcctt gctcatacaa ttcctccaaa
    12781 tctatagtaa ctattgtacc acttattttg tatccttcgt tttcttgttt tcaagagcta
    12841 gttatagttt caaactatat aaaaagggag tttttcctgc tccctttaat agactataaa
    12901 atggtgaatc tcaaaagaca ccttcactct atcatttgct cctgcacaaa acgagcataa
    12961 cgctcatgat tttccagtag ttccttatga gttcctgagc cagtgatttt cccctcctct
    13021 aagaagaaaa tacaatccac atcttttacc gttgacaaac gatgcgctat aatcacaacc
    13081 gtcttctcct ttaatacaga atagaggcta ctgataatcg catactcaga atccgcatca
    13141 agattagcag tggcttcatc aaatataaga atttcagcat cttttaagta ggctctagct
    13201 atttgaagtc tttggctttg cccccctgac aagagtcgtc cgcgttcacc aacttcagta
    13261 tctagtccct ctttcatgga gcgaatctca tcacctagtg atactaagtc tagcactttc
    13321 atcaattcat catcagttac taagcgattc aaaccgagac aaagattgtc acgaatactg
    13381 ccagataaga ctgcattatt ttgtgaaacc caagcgattt tacttctcca ttcttttaag
    13441 ttaaaatcat atatacttga ttgctccatt agaatatctc ctgaaagcgg tttataaaac
    13501 cgctctaaca aacgcacaat cgttgatttt cctgatccag atggtccaac aaaagcaatt
    13561 ttttgcccct tgaaaattga acaaataata tcctttaaga caggtcgatt ttcatcataa
    13621 ccaaaataga catggttaaa attcaaccct cgtcctgata ccgattttcc tccctcaaat
    13681 ttttctttag gaactgcaag caagttctcc agtgcaactg aagatccctt gctcctagaa
    13741 taaacagtta caaaattagc tatattacta ataggattaa gtaattgaaa gaggtaaatc
    13801 aaaaacgaaa ccaaggttcc cacagatata tatcctgcgc tgacccgata acccccatag
    13861 gttagcatca cagctatagt cgcaaagata aataagagag caaacggggt ctcaaaagaa
    13921 gtaatcctat ctgatttcag tgaattgttt tgtacccttt caatacaatt atccaaaaca
    13981 tcctgtacac ttttctctgc ttggttagtc ttaattaatt catgttcttg aatcttttca
    14041 gtcaattgcc ctgttaaaaa tccgatttcc tcctgtaaac gacgactata cttttcactg
    14101 atattggaaa ggggcaagat aataaacatc atacaaggaa gagtgatgaa taaaagtaga
    14161 gaaagattcc aatcaagact aaataagact acaatggaac caagtaccat aactaaactc
    14221 agaataatat ttgggaaagt cgtaattaaa aactcacgaa tgacactcgt gtcattgaca
    14281 atggcagaag tcaactcccc actttggctc ttatcaaaga aggatttctc tgcataaatc
    14341 aacccctcta tcactttttt cctgattttt gctatctttt ttcacccgat tgactaaaca
    14401 gatagtaacc aatagaagaa aacaaggctt gaccaataaa aatcaaaaac gattgaaata
    14461 ctttggaacc tatattttca atagaactcc catctattaa accttttaag ataaggggaa
    14521 gcaacaaagc aagtagacta gacagaacaa ggaagaaact ccccataatc accttagtat
    14581 ctactcttaa taattttaat ttcataaata ctccttataa tatttcaacg gataaagtcg
    14641 ggaataactc aatttgagga taaaatctaa taaatcttcc tataacaaaa cgcataacat
    14701 ctaggatttt atatacctga tattatgcgt ttttaagcac aaagacttct tacacaaact
    14761 tatctacaat tagattttat ttgacatgtt ttgccaattc ttcttgggct tttttattgg
    14821 attcttcttt ttctttcaac catttttctc tggcttttgc atattcgtct gttgtgacaa
    14881 tcttatcttg tactttgagg tatttatatg attcaacccc ttttgtaccg gttaaaccat
    14941 aggcagcagc aaatggtacg gttcttctca atgatggtgt tcccccacgc gaaacacttg
    15001 gaagaactaa agaactatca atcaaccaag cttgaatatc agcatatttc tcataacgtt
    15061 tggctggatc ttgctcttta ttagcttctt ccaacatttg agtatagaca tccagcccaa
    15121 cttccttagc cttgtcattg gcttctccag gctctagtcc aagattttgt agaactcctc
    15181 cactattagt attaaaaata tcgagatagg ttgacgggtc ttgataatca ggtccccaac
    15241 cgccatgata taaatcatag tctttctgag cagctgtttg agcaaagtag cctgaactgt
    15301 caaactcatc tgatgttaat tgctgaatgt caatcactac attatcagaa cctaaaacag
    15361 attcaattga ttgtttgata gaactaactc cttgtatgcc tactttatct gttacttcca
    15421 cagtcttatc caagtggatt gggaattgaa caccctttgc ttcgagttct ttcttagctt
    15481 ccgcaaactt agccttggct ttctcaggat tgtagtaagg gtcttgacca tccgcaaagt
    15541 tgataccttg ccattcctta ccatagttga ccatcttaga ggctacaact tcaccaaagt
    15601 cttttccctt gatactgaca aagtttggag gaaccactag gttacgcaaa atctttgttg
    15661 caccttcttt cccttcagac tgagccccat aagatgttct gtcaaaagca aaattgatag
    15721 cctgacggaa gtttttattg agaactgctt cctgagtcga tttcttttca atgtcacttg
    15781 ttttagaagt ataattgtaa gacttcctat ctaggttaaa attaaagaaa tatgaagttg
    15841 aattttgcat actatagatg atattgtttt tgtatttttc tttaatccct tcatagctgg
    15901 agctgttagg aaaaagacga gccgtagtat aagcaccagc tgtaaaatta cgttccagtg
    15961 attcttgatc gctaccatca tagtaggtca atttcacatc gtctacaaag acattcttag
    16021 catcccagta attagggttt ttcttatatt caatagcaga ttttgagaca agtgctttca
    16081 tcaagaaagg tccattgtac aaaatactag atggatccgc cttcccaaaa tcatcccctt
    16141 ttgatttcag gaaatctgca ttaacaggaa aaagtatcgt tgcaagtgtt tttgaattcc
    16201 agtaaagttc tggtttaacc aaagtatatt gaaccgtttg gtcatcaagt gccttgacac
    16261 cgacagttga aaagtcgctt gttttaccag tgatatagtc atccaaacca gcaacagagt
    16321 cctgcactag atacaaggct tctgattttt tatcagctgc atattgcaaa cctgtcacaa
    16381 aatcctgggc agttacaggc gcatattctt ctccctcaga agtaaaccac ttggcatcct
    16441 tacgaagttt gtaggtatag gtcaaaccgt cctgagaaac agtccaatcc tctgctaatg
    16501 atggaataat attcccatat tggtcatttt ctaataaccc gtctaccaaa tttgcaacaa
    16561 tatcggatgt tgctgcgcgg ttttctgcta gatagttcaa gctagatgga tcacttgaat
    16621 aaacatagtt gtaggttttt gacgccgtgc tagaatttcc acacgcgctc aataaaactc
    16681 ctgtacccag gacaagacct gccaaggtta gatatttgct cttagacttt ttcatttcca
    16741 gaacctcctg ctt
//
LOCUS       DAVLWA010000036        16744 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLWA010000036 DAVLWA010000000
VERSION     DAVLWA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.036"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            136..684
                     /locus_tag="VYN80_001156"
     CDS             136..684
                     /locus_tag="VYN80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1541074.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            794..1693
                     /locus_tag="VYN80_001157"
     CDS             794..1693
                     /locus_tag="VYN80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1541075.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEMAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1718..2992)
                     /gene="serS"
                     /locus_tag="VYN80_001158"
     CDS             complement(1718..2992)
                     /gene="serS"
                     /locus_tag="VYN80_001158"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1541076.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3206..3577)
                     /locus_tag="VYN80_001159"
     CDS             complement(3206..3577)
                     /locus_tag="VYN80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1541077.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3580..4944)
                     /locus_tag="VYN80_001160"
     CDS             complement(3580..4944)
                     /locus_tag="VYN80_001160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1541078.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLTAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5269..6054
                     /locus_tag="VYN80_001161"
     CDS             5269..6054
                     /locus_tag="VYN80_001161"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1541079.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6148..6582
                     /locus_tag="VYN80_001162"
     CDS             6148..6582
                     /locus_tag="VYN80_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1541080.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6582..7556
                     /locus_tag="VYN80_001163"
     CDS             6582..7556
                     /locus_tag="VYN80_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1541081.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7617..7841
                     /locus_tag="VYN80_001164"
     CDS             7617..7841
                     /locus_tag="VYN80_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1541082.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7960..8934
                     /gene="fabK"
                     /locus_tag="VYN80_001165"
     CDS             7960..8934
                     /gene="fabK"
                     /locus_tag="VYN80_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1541083.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            8927..9847
                     /gene="fabD"
                     /locus_tag="VYN80_001166"
     CDS             8927..9847
                     /gene="fabD"
                     /locus_tag="VYN80_001166"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1541084.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9881..10612
                     /gene="fabG"
                     /locus_tag="VYN80_001167"
     CDS             9881..10612
                     /gene="fabG"
                     /locus_tag="VYN80_001167"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1541085.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGTFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10634..11869
                     /gene="fabF"
                     /locus_tag="VYN80_001168"
     CDS             10634..11869
                     /gene="fabF"
                     /locus_tag="VYN80_001168"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1541086.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11872..12357
                     /locus_tag="VYN80_001169"
     CDS             11872..12357
                     /locus_tag="VYN80_001169"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1541087.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12354..12776
                     /gene="fabZ"
                     /locus_tag="VYN80_001170"
     CDS             12354..12776
                     /gene="fabZ"
                     /locus_tag="VYN80_001170"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1541088.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            12788..14155
                     /gene="accC"
                     /locus_tag="VYN80_001171"
     CDS             12788..14155
                     /gene="accC"
                     /locus_tag="VYN80_001171"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1541089.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14192..15058
                     /locus_tag="VYN80_001172"
     CDS             14192..15058
                     /locus_tag="VYN80_001172"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1541090.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15055..15822
                     /locus_tag="VYN80_001173"
     CDS             15055..15822
                     /locus_tag="VYN80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1541091.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKSLEELLEERYQRFRKY"
     gene            15980..16164
                     /gene="briC"
                     /locus_tag="VYN80_001174"
                     /pseudo
     CDS             15980..16164
                     /gene="briC"
                     /locus_tag="VYN80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
     gene            16182..16631
                     /locus_tag="VYN80_001175"
     CDS             16182..16631
                     /locus_tag="VYN80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541092.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIELYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKSWNWSFVFSLFATYVVYQILGNFWARYAHLINHRNIH
                     DEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
BASE COUNT         4793 a         3154 c         3887 g         4910 t
ORIGIN      
        1 tggaagtgtg ataaactaat ataacaacga aaatcttatg agagaataaa gctttatctt
       61 agataaactt aggaatcagg attttataag attttccaat aatattagtg tcaacaataa
      121 gaaaagaggt atcttatgac aacatttact atccatacag tagagtcagc accagcagaa
      181 gtgaaagaaa ttcttgaaac agtagaaaaa gacaacaatg gctatattcc caacctaatc
      241 ggtctcttgg ccaatgcccc gactgtttta gaagcctacc aaattgtctc atctatccac
      301 cgtcgcaaca gcctgacacc cgttgagcgt gaagtggtgc aaatcacggc agccgtgacc
      361 aatggttgtg ccttctgtgt cgcaggtcac acagcctttt ccatcaaaca aatccagatg
      421 aatgatgact tgattcaagc tcttcgcaat cgtactccaa ttgaaacaga tcctaaattg
      481 gataccctag ctaagtttac cttggcagtt atcaatacca agggtcgtgt aggagatgaa
      541 gccttgtctg agtttttaga agctggctac actcaacaaa atgccttgga tgtggttttt
      601 ggtgtcagcc tagcaatcct ctgtaactat gccaacaact tagctaatac accaattaat
      661 ccagaattgc aaccttatgc ctaattcata tctgagtaaa atgaagtctg aaataatcgg
      721 acttcgtttt tttaaatcct catttaagcg cttttatgtt atactgaaac taacatgatt
      781 attggaggtt aggatgaaaa aactcccctt agtattttca ggttgtttgc taggtttggc
      841 aggagctgga aatcttattt tagatacgtt gccagttcta tcccatcttt ttagtctgac
      901 tggtttggtt ttatggattt actttctaat tctgcatctc tttaattgga aagaaaccaa
      961 gcaagaattg accaagcccc ctcttttgtc aggaatggca acctttccta tggctgggat
     1021 gattttatcg acctatgtct ttcgcgtatt ctcttatctt cctttggtag cacaagggat
     1081 ttggtggttt tcatttctct tggatttgac cttgattgct ggttttacca tcaagtttgc
     1141 ttgtccaggg cggagggttc atgccactcc aagttggacg gttctctatg tggggatagc
     1201 agtggctgcc ttgacctatc ctctggtagg tattatcgaa atggcctatg cgaccctgag
     1261 ttttggtttt ctcttgacct tctatctcta tccccttatt tatagcgatt taaagaaaca
     1321 tccactccca ctagccttgc ttggacaaga aggaatctac tgtgctcctt tctctctact
     1381 cttggcttct ctagttcgag taggaggaac cagcctgccg acttgggtct tgattgtcat
     1441 gattttggct tctcaatcct tctttttctt tgttttaact cgtctgccca acattttaaa
     1501 acaaggtttt caaccagcct tctcagccct caccttccca accattatca cagcgacctc
     1561 gctcaagatg gctcagggaa ttttgaaact tccatttctg gattacctgg tattggctga
     1621 aaccattata tgcctaacta ttttattctt tgtactaggt gcttatctga tttggttacg
     1681 aaaaaaggtc tagctagaaa tagctaaacc ttatttttta tggtttgatg acttcagctc
     1741 cacccatgta tggacgaagt gcttctggga tggtcacaga accatcttca ttttggtaat
     1801 tttcaagaat tgcagccact gtacgtccaa ctgcaagtcc agaaccgttc aaggtatgaa
     1861 ggagtttcac cttgccatct gcttcatcac ggtaacggat ttgggcacga cgggcttgga
     1921 aatcttctgt gtttgaacag cttgagattt cacggtaatt gttttgtgct ggaatccaca
     1981 cttccaagtc gtaagtcttc gcagctgaga agcccatatc tccagtagag agagcaacga
     2041 cacggtatgg aaggttgagt ttttgaagaa tgttttcagc gttggctgtc attttttcca
     2101 attcttcgta agattcttct ggtttggcaa atttgaccat ttcaaccttg tggaattggt
     2161 gcaaacggat caagccacgc gtatcacgac cggcagaacc agcctcagaa cggaatgacg
     2221 gactcatggc agtgaagtag attggaagat ctttgccgtc taagatttca tcacggtagt
     2281 agtttgtcag aggaacttca gcagttggaa tcaagacaaa gttggtatcg ctgagttcaa
     2341 aagtatcttc cttaaatttt ggatactgac cagtaccaaa catagaatca tggttgacta
     2401 tgtaaggtgt gatgacttca gtatagcctt cttttccatg ttcatccaac ataaagttgt
     2461 agatagcacg ttccaaacga gcaccgaggc ctttatagaa gaggaagcga gcgcctgtta
     2521 ccttaccacc gcgttcccag tcaagaatac caaggtcttc accgagatcc cagtgagctt
     2581 taggttcgaa gtcaaactcg cgtggagtac cccaacggcg aacttccaca ttgtcgtctt
     2641 cgtcagcccc aacaggaacg ctgtcagctg ggatatttgg aagagtcgtt gtaaattctg
     2701 tcaatttagc atcgatttct gccaattcag catccaaggc tttaacctca gcagatagat
     2761 tttgcatggc agcaatcttg tcatctgtat tttccttgtt gcgcttagct tgggcaatct
     2821 cagcagaaac tgtgttacgt tctgctttga gagtttcaac cttgaccaag atgttacgac
     2881 gtttagcatc gatttctttc atttcattca agacagcagc atctacacca cgtgtagcta
     2941 atttttctgc gacagcttca aaatctgtac gaatacgttt gatatctaac ataagaactc
     3001 ctttatgaaa aaagcacgcc tgacaaaacg ttggagtggc agggccacgg ttccatccaa
     3061 cttcacaggt gtgcacttga ttgtgtatgt aattgttact aacggtagaa tttcacctat
     3121 ccctcctatc tgctcgcagt acccgcagac tttctgaaag aagaagataa cctacttatc
     3181 cgttgctatg attatactaa agtttctact tttttgcaaa tagattttta aatttttggc
     3241 taattgtctg aatcagggtc ggaagtttga cgaccttgtc attgcctagt ttttcgcgtg
     3301 caattttgag aatggcacct gagtcttttg aagcaaagag gaattttcct ttgtctgtaa
     3361 agacttcgaa gtggcggctg attttgcgtc cagtgacatt ggctccaatc tgattgatat
     3421 ggctccaagg aatctggata aattgttcga cattgacatc tgggtaaaat tccaaagcct
     3481 gatctccgac aaggaatttc ccaactttcc cagcgataga gaggtaggaa gtgcctgtcg
     3541 tactgaggag tactgttttg ttaagtgatt gggccatgct tagtcttcct tactttctcc
     3601 aaaaaaggca ttgtagaggg ctttaattgc tgctttctct tggtctttat tgacaacaaa
     3661 cataatagaa acttcactag aaccttgaga catcatctgg atgttgattt tgttttcaga
     3721 tagagcgcgt gtcgcagtag cagtcactcc gatgtggctc ttcatttttt caccaacaat
     3781 cataatgata gaaaggtcgt gttcgatttc tgcatgatct actttagcct tttgaaccaa
     3841 ctgacgcagg atttcttctt ccttgatggg agttagttgg cgagaacgga gaatgataga
     3901 aagatcgtcg atacctgttg gcatatgttc ccaaccgatg ttaagttctt ccaggatttg
     3961 cagaaccttg cggccaaatc caacctcacg gttcatgagg tatttcgaca tgttaatgct
     4021 gacaaagcct gagtcaccag caattcccac aactggaaat tcatcattac tgtgttttag
     4081 aacgatacga gtacctggat ggtcagggtt gttggtattc ttgataacca gaggaatttt
     4141 tccacggtag gcaggaagaa gagcctcgtc atgaaggact gagaagcctg cataggccaa
     4201 ctcgcgcatt tcacggtagg tcaactcagg aatcgagtgt ggttggtgga taataccagg
     4261 gtgggctgca aagataccat caacgtccgt aaagttttca tagaggtcgg ctttgacacc
     4321 agcagcaatg atagaacctg taatatcaga acctccacgt gagaaagtac agatttgatt
     4381 ttccttagtg acaccaaaga aaccaggaat gacaaggact tcattggtgt ttgtcaattc
     4441 ttcaatcttg tcataacttg atggaatgat gcgagcgtga ccaggttcac ttgtgaccac
     4501 aatcccagct tctctagggt gcatatagcg tgcatcgata ccattttggt taaagtaggc
     4561 agcagtcaat ttggcattgt tattttcacc ggctgctagg aaagtatcgt agagaaattc
     4621 attttcttca ataggaagag tggccaaggc gtgaatgctt ttagaaattt tttctagcac
     4681 agctggtttt agtcccaatt cactaaccat agcagcatag cggtcgataa tccagctttg
     4741 gctcttgcta atatcgttac ccgcaacata gtcgcggtag tatttaatca gggcatccgt
     4801 aaccttagta tcttcagcat tgcgtttacc aggcgcagaa acgactacaa aacgacgctc
     4861 tgaatcgctt ttgacgatgt ttaaaacttt ttctaattga ctagcagagg caagagaact
     4921 acctccaaat ttaacaactt tcataagaac tcctaaagta agtattttat acgattatag
     4981 cagaaagaaa ggcttttttc aatgaagaaa ataagatgct ttctagccta aagcgagcct
     5041 ttcttatcgg ctgagacact ttcagtcgat tttttcttgc tttctgctcc taaaaaagat
     5101 atactttgac agtgaaataa tttaacactc aatgaaaatc aaagagcaaa ctaagaagct
     5161 agacgaagtc agctcaaaat actgttttga ggttgcagat ggaagctgac gcggtttaaa
     5221 gagattttcg aagagtataa actgcttata aaataaaaag gagccctgat ggaacacatt
     5281 atttatcagc ttgaagagga cttggcaatc cttaccttga accgtcctga ggtcgcaaat
     5341 ggttttcata ttcccatgtg tgaggagatt ttagaagctc tgactttggc agaagaaaat
     5401 ccagctgtgc attttatctt aatcaatgcc aatggaaagg tcttctcagt tgggggagat
     5461 ttggtagaga tgaagcgggc agtggatgag gatgatattc catcattgac aaaaatcgca
     5521 gagttggtca atacgatttc ttataaaatc aagcaaatag ccaaacctgt tttaatggaa
     5581 gttgacggtg ctgttgcagg tgccgcagcg aatatggctg ttgcggcaga tttctgtttg
     5641 gcgacggata aggctaagtt tatccaagcc tttgttggtg ttggtttggc tccagatgca
     5701 gggggaattc atctcttgag ccgcagtatc ggtgtgacgc gtgctgctca attagctatg
     5761 acaggagagg ctttaacggc agaaaaagcc ctagaatggg ggttggttta ccgcgtttct
     5821 gaagctgaaa aacttgaaaa gacgagagaa cagcttctta aaaaattaag acgtgcttca
     5881 agtaattcct atgctgccat taagaagttg gtttgggaga gccagtttaa agactggcag
     5941 ggttacgcta ctttagaact gaacctacag aaatccttag ctcaaacaga ggatttcaaa
     6001 gaaggagtgc gagctcattc ggagagaaga agacctaaat ttacaggaaa ataaaaaata
     6061 cttgcatcat tctttgaatt ttgatataat tctttctgtc aaatgttttg attgtaaaag
     6121 ttttttgaag aaggagggat atgaaaaatg gactaccaac gaatcaatga atatttaaca
     6181 tctatattta acaatgtcct tgtaattgag gaagtgaact tgagaggtag tcgttttaag
     6241 gatatctcca tcaaagaaat gcatacgatt gatgtcatcg gtaaggctcc agacgtgact
     6301 ccaagtcaag tgtcaaaaga gttgatggta actcttggaa ctgttacgac aagtttgaac
     6361 aatttagagc gtaagggcta cattgagcga gttcggtcag aacaggatcg tcgtgtggtg
     6421 catctgcatt tgacaaagaa gggtcgcttg attcataggc tgcataaacg cttccacaag
     6481 gccatggtag aaaaaattat tgatggcatg agcgaggaag aaattgctgt catgggtaaa
     6541 ggtttgacta atctttacca atttttggag gatttgaaat aatggctttt gcaaaaataa
     6601 gtcaggttgc tcattatgtg ccagagcaag tggttacaaa tcacgacttg gctcagatta
     6661 tggataccaa tgatgagtgg atttcaagtc gaacgggaat acgacaaagg catatttcaa
     6721 gaacagaatc taccagtgat ttggctacag aggttgctaa gaaactgatg gcaaaagctg
     6781 gaataacagg agaagaactg gattttatca tcctagctac cattactcca gattcgatga
     6841 tgccctctac agctgctcgt gttcaagcta atattggtgc taataaagcc tttgcttttg
     6901 acttaaccgc ggcttgcagt ggatttgtat ttgctctttc aactgctgaa aagtttatcg
     6961 cttctggtcg ctttcaaaaa ggcttggtga ttggtagtga aaccctctct aaggcagtcg
     7021 attggtcgga tcgatcaaca gctgtgttgt ttggagatgg tgctggtggt gtcttgttag
     7081 aagctagcga gcaagagcat ttcttagctg agagtctcaa tagcgatgga agtcgcagcg
     7141 agtgtttaac ttatgggcat tcaggtttgc attctccatt ttcagatcaa gaaagtgcag
     7201 attcgttttt gaagatggat ggacgcacag tctttgattt tgccattcga gatgtagcca
     7261 agtctatcaa gcagactatt gatgaatctc ctatagaggt gacagacttg gattatctgc
     7321 tacttcatca agccaatgac cgtattttgg ataagatggc tagaaaaatt ggtgttgacc
     7381 gagccaaact tccagccaat atgatggaat atggcaatac cagtgcagcc agtatcccga
     7441 ttttactttc agagtgtgta gaacaaggtc tcatcccttt agatggtagc cagactgttc
     7501 ttctatcagg cttcggtgga ggcttgacct ggggcacgct cattcttaca atttaggtaa
     7561 tcatgtggtg aacacattgt tataaaaaaa actatttatt ttaaaggagt cctatcatgg
     7621 cagtatttga aaaagtacaa gaaattatcg ttgaagaact tggaaaagac gcatcagaag
     7681 taacacttga atcaactttt gatgatttgg acgcagattc attggacttg ttccaagtaa
     7741 tctcagaaat cgaagatgct tttgatatcc aaatcgaagc agaaaatgac ttgaaaacag
     7801 ttggtgactt ggttgcttac gttgaagagc aagcaaaata agcagaatat tagtagaagg
     7861 agtagggaga aacccactcc ctcttgttta ggtaatagtt tgactgtcaa attatggtga
     7921 aatcgaacta ttacgtaagc aaggaaggtt ggaggcacta tgaaaacgcg tattacagaa
     7981 ttattgaaga ttgactatcc tattttccaa ggagggatgg cctgggttgc tgatggtgat
     8041 ttggcagggg ctgtttccaa ggctggagga ttaggaatta tcggtggggg aaatgccccg
     8101 aaagaagttg tcaaggccaa tattgataaa atcaaatcat tgactgataa accctttggg
     8161 gtcaacatca tgctcttatc tccctttgtg gaagatatcg tggatctcgt tattgaagaa
     8221 ggtgttaaag ttgtcacaac aggagcagga aatccaagca agtatatgga acgtttccat
     8281 gaagctggga taatcgttat tcctgtcgtt cctagtgtcg ctttagctaa acgcatggaa
     8341 aaaatcggtg cagacgctgt tattgcagaa ggaatggaag ctggggggca tatcggtaaa
     8401 ttaacaacca tgaccttggt gcgacaggta gccacagctg tatctattcc tgttattgct
     8461 gcaggaggaa ttgcggatgg tgaaggtgct gcggctggct ttatgctagg tgcagaggct
     8521 gtacaggtgg ggacacggtt tgtagttgca aaagagtcga atgcccatcc aaactacaag
     8581 gagaaaattt taaaagcaag ggatattgac actacgattt cagctcagca ctttggtcat
     8641 gctgttcgtg ctattaaaaa tcagttgact agagattttg aactggctga aaaagatgcc
     8701 tttaagcagg aagatcctga tttagaaatc tttgaacaaa tgggagcagg tgccctagcc
     8761 aaagcagttg ttcacggtga tgtggagggt ggctctgtca tggcaggtca aatcgcaggg
     8821 cttgtttcta aagaagaaac agctgaagaa atcctaaaag atttgtatta cggagccgct
     8881 aagaaaattc aagaagaagc ctctcgctgg acaggagttg taagaaatga ctaaaacagc
     8941 ctttttattt gctggtcaag gtgcccagta tctagggatg ggacgggatt tctatgatca
     9001 gtatccgatt gttaaagaaa cgattgatcg agcgagtcag gtgctcggtt atgatttgcg
     9061 ttatctcatc gatacggaag aggacaaact caatcagacc cgctatacgc aaccagccat
     9121 tctagcgact tcggttgcta tctaccgttt attgcaagaa aagggctatc agcctgatat
     9181 ggttgctggt ttgtctcttg gagaatactc tgccttggtg gcaagcggcg ccttggattt
     9241 tgaagatgcg gttgccttgg tagctaagcg tggagcctat atggaagaag cgactcctgc
     9301 tgactctggc aagatggtag cagttctcaa tacgccagta gaggtcattg aagaagcctg
     9361 tcaaaaagct tctgaacttg gagtggttac tccagccaac tataacacac ctgcacaaat
     9421 cgtcattgct ggagaagtgg ttgcagttga tcgagcggtt gaacttttgc aagaagcagg
     9481 tgccaaacgc ttgattcctc ttaaggtgtc aggtcccttt cacaccgctc tccttgagcc
     9541 agctagccag aaactagctg aaactctagc tcaggtaagt ttttcagatt ttacttgtcc
     9601 cctagtcggc aatacagaag ctgctgtgat gcaaaaagag gacattgctc agctcttgac
     9661 gcgtcaggtc aaggaacccg ttcgtttcta tgaaagtatt ggggtcatgc aagaagcagg
     9721 cataagcaac tttatcgaga ttggaccggg gaaagtcttg tcaggttttg ttaaaaaaat
     9781 tgatcaaact gctcacttag ctcatgtgga agatcaagcg agtttagtag cacttttaga
     9841 aaaatagact aaaataagta gaagttttga aaggaaaaaa atgaaactag aacataaaaa
     9901 tatctttatt acaggttcga gtcgtggaat tggtcttgcc atcgcccaca agtttgctca
     9961 agcaggagcc aacattgtct taaacagtcg tggggcaatc tcagaagaat tgctcgctga
    10021 gttttcaaac tatggtatca aggtggttcc catttcagga gatgtatcag attttgcaga
    10081 cgctaagcgt atgattgatc aagctattgc agaactgggt tcagtagatg ttttggtcaa
    10141 caatgcaggg attacccaag atactcttat gctcaagatg acagaagcag attttgaaaa
    10201 agtgctcaag gtcaatctga ctggtacctt taatatgaca caatcagtct tgaaaccgat
    10261 gatgaaagcc agagaaggtg ctatcattaa tatgtctagt gttgttggtt tgatggggaa
    10321 tattggtcaa gctaactatg ctgcttctaa ggctggcttg attggcttta ccaagtctgt
    10381 ggcacgcgag gtcgctagtc ggaatatacg agtcaatgtg attgctccag gaatgattga
    10441 gtctgatatg accgctatct tatcagataa gattaaggaa gctacactag ctcagattcc
    10501 gatgaaagaa tttgggcagg cagagcaggt tgcagatttg acagtatttt tagcaggcca
    10561 agattatcta actggtcaag tgattgccat tgatggtggc ttaagtatgt agcgaaagct
    10621 agaggtgaaa agaatgaaac taaatcgagt agtggtaaca ggttatggag taacatctcc
    10681 aatcggaaat acaccagaag aattttggaa tagtttagca actgggaaaa tcggcattgg
    10741 tggcattaca aaatttgatc atagtgactt tgatgtgcat aatgcggcag aaatccaaga
    10801 ttttccgttc gataaatact ttgtaaaaaa agataccaac cgttttgata actattcttt
    10861 atatgccttg tatgcagccc aagaggctgt aaatcatgcc aatcttgatg tagaggctct
    10921 taatagggat cgttttggtg ttatcgttgc atctggtatt ggtggaatca aggaaattga
    10981 agatcaggta cttcgccttc atgaaaaagg acccaaacgt gtcaaaccaa tgactcttcc
    11041 aaaagcttta ccaaatatgg cttctgggaa tgtagccatg cgttttggtg caaacggtgt
    11101 ttgtaaatct atcaatactg cctgctcttc atcaaatgat gcgattgggg atgccttccg
    11161 ctccattaag tttggtttcc aagatgtgat gttggtggga ggaacagaag cttctatcac
    11221 accttttgcc atcgctggtt tccaagcctt aacagctctc tctactacag aggatccaac
    11281 tcgtgcttcg atcccatttg ataaggatcg caatgggttt gttatgggtg aaggttcagg
    11341 gatgttggtt ttagaaagtc ttgaacacgc tgaaaaacgt ggagctacta tcctggctga
    11401 agtggttggt tacggaaata cttgtgatgc ctaccacatg acttctccac atccagaagg
    11461 tcagggagct atcaaggcca tcaaactagc cttggaagaa gctgagattt ctccagagca
    11521 agtagcctat gtcaatgctc acggaacgtc aactcctgcc aatgaaaaag gagaaagtgg
    11581 agctatcgta gctgttcttg gtaaggaagt acctgtatca tcaaccaagt cttttacagg
    11641 acatttgctg ggggctgcgg gtgcagtaga agctatcgtc accatcgaag ctatgcgcca
    11701 taactttgta ccaatgacag ctgggacaag tgaagtatca gattatatcg aagctaatgt
    11761 cgtttatgga caaggcttgg agaaagaaat tccatacgct atttcaaata cttttggttt
    11821 tggaggccac aatgcagttc ttgctttcaa acgttgggag aatagataag tatgaattta
    11881 aacgatatta aagacttgat gactcaattt gaccagtcaa gtttgagaga attttcttat
    11941 aaaaatggga cggatgagtt gcagtttagc aagaatgaag caagacctgt gcctgaagtt
    12001 gcaactcaag tcgctccagc acccgttcta gcaacaccga gtccagtagc tcctacatct
    12061 gctccagcag agactgtagc agaagaagtt ccagctccag ctgaagcaag tgtggctact
    12121 gagggaaatc ttgtagagag tccacttgtt ggagtggttt acttggctgc tggaccagat
    12181 aaacctgcct ttgttacagt tggtgatagt gtcaaaaaag gtcaaacatt ggtaattatc
    12241 gaagccatga aagtcatgaa tgaaatccca gctcctaagg atggtgtggt aacggaaatt
    12301 ctcgtctcta acgaagaaat ggttgagttt ggtaaaggat tggtacgtat caaatgatcg
    12361 atattcaagg aatcaaagaa gctcttcccc accgttatcc tatgcttcta gtggaccgtg
    12421 tcttggaagt gagcgaggat accattgttg ctatcaaaaa tgtgaccatt aacgaacctt
    12481 tctttaacgg ccactttcct caatacccag ttatgccagg tgttctgatt atggaagcct
    12541 tggcgcaaac tgctggtgtg ttggagttat caaaacctga aaataaagga aaactggtct
    12601 tttacgctgg tatggacaag gttaagttca agaagcaagt tgtaccaggc gaccaattgg
    12661 ttatgacagc gacttttgta aaacgtcgtg gcaccatagc tgtggttgaa gcaaaggctg
    12721 aagtggatgg caagcttgca gccagtggta cccttacttt tgcaattggg aactaaggag
    12781 gttctccatg tttcgaaaaa ttttaattgc caatcgtggt gaaattgcgg ttcgtattat
    12841 ccgtgcggca cgtgaattgg ggattgcgac ggtagcggtt tattcaactg ctgataagga
    12901 agctcttcat acgctgttgg cagatgaagc agtttgtatt ggtcctggca aggcaacaga
    12961 gtcttatctc aatattaatg cagttctatc agctgcagtc ttgactgagg cagaagctat
    13021 tcaccctggt tttggatttc tcagtgaaaa ttccaaattt gcgaccatgt gtgaagaagt
    13081 aggtatcaag tttatcggtc catctggtca tgttatggat atgatggggg ataagatcaa
    13141 tgcgcgtgct cagatgatta aagcaggtgt gcctgttata ccaggttcag atggagaagt
    13201 gcataactct gaagaagctt tgattgttgc tgaaaaaatt ggctatcctg ttatgctcaa
    13261 ggcttcagca ggtggaggtg gtaaagggat tcgtaaggtt gaaaaaccag atgacctcgt
    13321 ttctgccttt gaaactgcct ctagtgaggc caaggccaat tatggcaatg gtgccatgta
    13381 catagaacgg gttatctatc cagctcggca cattgaggtt caaatcctag gtgatgagca
    13441 tggacatgtg attcacttgg gtgaacggga ttgttctctt caaaggaata accaaaaggt
    13501 tttggaagaa agtccctcga ttgcaatcgg aaaaacgctg cgtcatgaaa taggtgctgc
    13561 tgctgttcga gcggcagagt ttgttggcta tgagaatgca ggaaccattg aatttcttct
    13621 tgatgaagca agtagcaatt tctatttcat ggagatgaat actcgtgttc aggtagaaca
    13681 tccagtaaca gagtttgttt caggtgttga tatcgttaag gaacagattt gcattgcggc
    13741 aggtcagcct ttgtctgtta agcaagaaga tattgtccta cgcggtcatg ccatcgagtg
    13801 tcgtatcaat gcagaaaacc cagcctttaa ctttgctcca agtccaggta agattactaa
    13861 cctctatctg ccaagtggtg gagttggctt gcgcgtggat tcagcagttt atccaggtta
    13921 taccattccg ccttattatg atagtatgat tgccaaaatc atagtacacg gcgaaaatcg
    13981 ttttgacgcc ttgatgaaaa tgcaacgtgc cctctatgaa ttagagattg aaggagtgca
    14041 gaccaatgca gatttccagc ttgacctcat ttcagatcgc aatgtcattg ctggggatta
    14101 tgatacttcc ttcttgatgg aaaccttctt acctaaatat caagaaaaag aataaaatga
    14161 ctttaagagt ttaaaccgaa aaggggaatc aatggctcta tttagtaaaa aagataagta
    14221 tattcgaatc aatcccaatc gttcggttag ggaaaaacct caagctaagc cagaggttcc
    14281 agatgaatta ttttcccagt gtccaggctg taagcatacc atctatcaga aggatctggg
    14341 aagtgaacgt atctgtccgc actgtagcta tacctttcgt atttctgccc aagaacgctt
    14401 ggctttgacg attgatatgg gaaccttcaa agaattgttt acagggattg aaagcaagga
    14461 tcccttgcat ttccctggtt accaaaagaa actggcatct atgcgtgaaa aaacaggtct
    14521 gcatgaagcc gttgtgacag gaactgctct tattaaaggt cagactgtgg ctcttgggat
    14581 tatggattct aactttatca tggcttctat gggtacggtt gtaggtgaaa aaatcactcg
    14641 tttgtttgag tatgcgactg tcgaaaaatt gccagttgtc ctattcacag cctctggtgg
    14701 agcccgtatg caggaaggaa tcatgagtct catgcagatg gctaagatct ctgcggcggt
    14761 taaacgtcat tcaaatgctg gtctctttta cctgaccatt ttgacagatc caacgactgg
    14821 tggtgtgaca gcttctttcg ctatggaagg cgatatcatt ctggctgaac cacagagctt
    14881 ggttggtttt gctgggcgtc gtgtgattga aaatacggtt cgtgaaagct tgcctgagga
    14941 tttccaaaag gcagaattcc tattagaaca tggctttgtg gatgctattg tcaaaagaag
    15001 agacttacca gatacgattg ctagcctagt cagattgcat ggagggagtc ctagatgaat
    15061 attgcaaaaa tagtcagaga agcgcgtgag cagagtcgct tgacaacctt ggactttgcg
    15121 acaggcattt ttgatgaatt tatccaatta catggtgacc gttcttttcg tgatgatggt
    15181 gcagttgttg gtggtattgg ttggcttgga gaccaagctg taacagtggt tggtatccaa
    15241 aaaggcaaga gtttgcaaga caacctcaaa cggaattttg gccaaccaca tccagaaggc
    15301 taccgaaagg cactgcggtt gatgaaacag gctgagaaat ttggccgtcc agttgtgacc
    15361 tttattaata cagcaggtgc ttatcctggt gtcggagcag aagaacgtgg tcaaggggaa
    15421 gctatcgctc gcaatctcat ggaaatgagt gacctgaaag ttcctattat cgccattatt
    15481 atcggtgaag gtggttcagg cggggctctg gctctagctg tcgcggaccg tgtctggatg
    15541 ctggaaaatt ctatctatgc cattctcagt ccagaaggct ttgcttccat tttatggaag
    15601 gacggtactc gcgccatgga agcagcagaa ctgatgaaaa tcacttcgca tgaactgtta
    15661 gaaatggacg tggtggataa ggtgatttct gaagtaggac tttctagtaa agaactgatt
    15721 aagagtgtca aaaaagaact ccaaacggag ctggctagac tttcacaaaa atcgctagaa
    15781 gagttgctgg aagaacgcta tcaacgattt agaaaatact aagaatgact aaatgagact
    15841 agaaaggtct cattttttat ggaaaaatgc aattcaggtt gaaaaattga tagttgagga
    15901 gatgggctat aaaatagaca aaaaagaaat atactataaa tgtgagccta caacaatcta
    15961 ttttaagaag gtacttgcaa tgacaggtac agaaacattt acagttattt caactgagga
    16021 cttggagcaa acttcaggtg gtcttgctgt ttgggaagat ggatatagta gatggttata
    16081 ttatagagaa tttgctccct atatgaggca agggcactta attcttatat agatgcttgg
    16141 aagtacggct tccgaacagg gtaatctttc aaaaaagtac gatgagaaac acaaagaaac
    16201 taagacaatt tggaatattt ttgcttatta ttttactatc tacctattta ccacaaacaa
    16261 ttgaattgta tgtgacgatc attttagggt taggagctga tgtctattct cttattttaa
    16321 caatggtatt agtgggaagt ttccttctat taatttggag gttaaaaaag aaaaagagct
    16381 ggaactggtc atttgtcttc tccttatttg caacttatgt ggtttatcag attctcggta
    16441 atttttgggc acgctatgct catttgatta atcataggaa tattcatgat gagtatttta
    16501 ccgtggttct ttcaaatgga caaccaacct ttttatcgac tatcttatct tttgtcctgc
    16561 ctgtaatcat cggtcctgtt tttgaggaga ctcttgatag agggtatttt atgaatacct
    16621 tctttcccta gtccaagtat tatttagatg tcatcttatc aggccttatc tttgggctta
    16681 gtcatttgat actatctcac agagatccaa ttagtttatt atattatagt ctgattggtt
    16741 tctt
//
LOCUS       DAVLWA010000037        16493 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLWA010000037 DAVLWA010000000
VERSION     DAVLWA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.037"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            142..1902
                     /locus_tag="VYN80_001176"
     CDS             142..1902
                     /locus_tag="VYN80_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1541093.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSG
                     VSGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNDQTGQQDTGDNDADY"
     gene            1889..3205
                     /locus_tag="VYN80_001177"
     CDS             1889..3205
                     /locus_tag="VYN80_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1541094.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3380..4366
                     /locus_tag="VYN80_001178"
     CDS             3380..4366
                     /locus_tag="VYN80_001178"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1541095.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4430..4501
                     /locus_tag="VYN80_001179"
     tRNA            4430..4501
                     /locus_tag="VYN80_001179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4463..4465,aa:Arg,seq:ccg)
     gene            4684..5382
                     /locus_tag="VYN80_001180"
     CDS             4684..5382
                     /locus_tag="VYN80_001180"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1541096.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5373..8912
                     /gene="smc"
                     /locus_tag="VYN80_001181"
     CDS             5373..8912
                     /gene="smc"
                     /locus_tag="VYN80_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1541097.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQDEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRQQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8909..9703
                     /locus_tag="VYN80_001182"
     CDS             8909..9703
                     /locus_tag="VYN80_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV1541098.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAGNGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9703..10521
                     /locus_tag="VYN80_001183"
     CDS             9703..10521
                     /locus_tag="VYN80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1541099.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            10525..11814
                     /gene="ftsY"
                     /locus_tag="VYN80_001184"
     CDS             10525..11814
                     /gene="ftsY"
                     /locus_tag="VYN80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1541100.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11858..13345)
                     /locus_tag="VYN80_001185"
     CDS             complement(11858..13345)
                     /locus_tag="VYN80_001185"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1541101.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13494..14234)
                     /locus_tag="VYN80_001186"
     CDS             complement(13494..14234)
                     /locus_tag="VYN80_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1541102.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14234..16399)
                     /locus_tag="VYN80_001187"
     CDS             complement(14234..16399)
                     /locus_tag="VYN80_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1541103.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5146 a         2940 c         3656 g         4751 t
ORIGIN      
        1 aaagttagtt tagaagattt ttataaatgg tatagtctaa ataaagaaga gttattaaat
       61 aaggcaactg ttggtgaaaa gtttaatgat aaattaaaag aagagtttct ccaggaatgg
      121 cctttggata ggattttaac aatgtcaatc gatgaatatg taataggaaa gggacagcaa
      181 aataagtctt tatgctacgc tcttgagaag ggaaaataca aaaatctatt tcttggaatt
      241 tctggtggct cagcttcaaa atttggtatt tattggaata aaaaaacaaa caaatataaa
      301 gatcaagcta ataatgagat ttcagagttg gatcagcgat tttcaaaatt aaaatcagat
      361 ttgtatgaaa ttatcaaaga aggtattcgt tttaactttg aaaatcctat ttttgatatg
      421 aaaagatcaa caaatgaatt tattggtcgt tctgctatgg tgacaaaatt actttgtatc
      481 tatactgagg gagatccttt ctttggtgta aatattaata gtcagaaaga attttggaac
      541 cactttgttt ctcagacaaa tcaaggtgga ccttatctgc aaaatcataa aataattgaa
      601 ctagtgtcca aaacttatcc tgagttggag ccatcgaaat taggaactat gctttttgag
      661 tattctaagc tttttatgga aaataaggaa gacaatagta caatggattc atcaaacaat
      721 tttcgtcatc aattaactca atctctatta aagtctccaa acctcatcct ccgtggtgct
      781 cctggcacag gaaagactta tcttgctaaa gaaattgcca aagaattaac ggatggcaac
      841 gaagaccaaa tcggatttgt acaatttcac ccatcctatg attatacgga ttttgtagaa
      901 ggtttaagac cagtatcaaa tggggatgga gctattgagt ttaggctaca ggacggtatt
      961 tttaaagatt tttgtcagaa agcaaaagaa acccaattga ttggaggaca agataatttt
     1021 gatgaggctt gggattctta cttagaatat ataaatgttg ctgaagaaaa agaatatata
     1081 acaaaaacat cttacttatc tattaatagt agacaaaatt tgtcagtaaa ttatgatagt
     1141 ggtgtttcag gatggtcact acctagcaaa tatgtttacg agttgtataa agataaaaat
     1201 tataataagc aagaatacta caaaagtggt ggaaaaactg tcctagaaac attgagaaag
     1261 agatttggtt tgaaagacta tgtttcccca acagaaattg atactgataa gaaattcgtc
     1321 ttcatcatcg atgaaatcaa tcgtggggag atttctaaga tttttggcga actctttttc
     1381 tctatcgacc ctggctatcg tggtgaaaaa ggaagtgttt ctacccaata tgcaaatcta
     1441 cacgaaactg atgaaaagtt ctatatcccc gaaaatgttt acatcatcgg aactatgaat
     1501 gatattgatc gttcagtgga tacctttgat tttgctatgc gtcgtcgttt tcgttttgtt
     1561 gaagttactg ccgagggtca agttggcatg ttggataaag agctgaatat ccatgcagaa
     1621 gaagcaaaaa ttcgtctaag aaacttgaac gctgctatcg aaaatgttca ggaattaaac
     1681 agtcattatc atattggacc aagttatttt cttaagttga aggatgtaga ttttgactat
     1741 gaattactct ggtctgatta tattaagcct ctcctagaag actacttgcg aggttcttat
     1801 gatgaggttg aaactttgga aactttgaaa aaagcatttg aactgacaaa taatgatcaa
     1861 actggtcaac aagatactgg tgataatgat gcggattact gataatcaat acaagattat
     1921 taaagaaaaa tttgttgaag aatatcctaa actaagcaat cttcttttag acagaacctt
     1981 ggaaagtcta tcccaagatg aacgtatttt catttttcca aatgatttga ctcatactcc
     2041 tgatttggat aaggaccaaa agatttttga aacagtcaat cagaaaatca agacagggaa
     2101 cgtgattggt tttcttggat atggtcagga aagattaacg atttcctcac gattttctga
     2161 tgagagtaat gaccattttt tgcattatct cttaaacaag gttcttcata tcaatctcac
     2221 tagtttagat gttgctttgt ctcgtgaaga gaggctttat caacttttga tgtatctctt
     2281 ccccaagtat ctacaagctg ctattcgaaa aggtctttat aaggaatacc agagattttc
     2341 tcataacgac agtcatgtta agggagtgat tgatgtaaga aaccatctca agaaaaatct
     2401 tcctttcacg ggaaatattg cctacacaac gagagagttc acctatgata atcccctcat
     2461 gcagttggtc cgtcacacta ttgaatacat taagaatcag aaaagcattg gtcaaggggt
     2521 actagataat ctctcaacta gtcgtgaaaa cgtatctgaa atcgtgcgtg taacgccctc
     2581 ttataaacta gctgatcgtg ctaagattat tcggggaaat caatctaaac ctatacgtca
     2641 tgcatacttt cacgagtaca gaaacttaca agaactttgt ctgatgatcc taaaccaaga
     2701 aaagcacggt ttagggtatc aagatcaaaa aatctatggt attctctttg atgttgcctg
     2761 gctttgggaa gagtatgttt acaccttgtt gccaaaaggt tttgtacatc ccagaaataa
     2821 ggataagacg gatggaattt cagtattttc tgttgggaaa cgaaaagtat atccagattt
     2881 ttatgacaga gaacgaaaga ttgttctaga tgcaaaatat aaaaaactgg aattgactga
     2941 aaaaggaatc aaccgtgagg acttattcca gctgatttcc tattcttata ttttaaaagc
     3001 tgagaaggct ggacttgttt ttccgagcaa ggacaaggtt attgataatg agatgggaaa
     3061 cctagcaggt tatggagctt tgttgaagaa atggtctatc caaattcctc agaagtcttc
     3121 atcttatagt gcattttgta aaatgatgga aaattcagaa gaaaatttta aagcgattat
     3181 tgatgaagaa gtggggagaa aataatttcc tctctttctt ttatttttaa cacaagtaaa
     3241 acccgaacaa tatatttcaa ttcataaaat aatttgacaa aaactgtact ttggtttata
     3301 ataaattaag gaaacgtcgg aaaagacgta gtgatgcgag agcataggta ggtcattaca
     3361 aaagaaacga gacatcgata tgttaaatga atttccaatt tttgattacg aagatattca
     3421 attgattcca aataaatgtg tcattaaaag ccgtgcagaa gcggatacaa gtgtcacttt
     3481 aggaaatcac acctttaaac tacctgttgt gccagcgaat atgcagacga ttttggatga
     3541 aaatgtagca gagcaactgg ctaaaggtgg ttacttctac attatgcacc gttttgatga
     3601 ggcaggacgc attcctttta ttaaacgaat gcacgatcaa gggctcattg cttctatctc
     3661 tgtcggtgtt aaggactatg agtatgattt cgttagacag ctcaagactg atgctcctga
     3721 gtatatcact attgatattg ctcatggtca tgcggatagc gtgatttcta tgattcaaca
     3781 catcaagaaa gaattgccag atacctttgt cattgctgga aatgtgggaa caccagaagc
     3841 tgtgcgtgaa ttggaaaatg ctggtgcgga tgctactaag gtcggaatcg gtcctggtaa
     3901 ggtttgtatc accaaggtta agactggttt tggtacaggt ggttggcagt tggctgctct
     3961 acgctggtgt gccaaggctg cgcgtaaacc gattatcgct gatggaggaa ttcgtactca
     4021 cggtgatatt gccaagtcta tccgcttcgg tgctagcatg atcatgattg gttccctctt
     4081 tgcaggacac attgaaagtc cagggaaaac gattgaagtc gatggtgaac agttcaaaga
     4141 atattatggt tcagcctcac aatatcagaa aggtgcttac aaaaatgtgg aaggcaaacg
     4201 tatcttgctt cctgctaaag gtcatctgca agacacttta actgagatgg aacaagacct
     4261 tcaaagtgct atttcttatg caggtggacg tcaggttgct gaccttaaac acgttgatta
     4321 tgtgatcgtg aaaaactcaa tctggaatgg ggatgcttcc cactaagacg gggtctttga
     4381 ctaaaaaact tgttattaga gcaaatttct gttataataa aacaagtttc cacccttagt
     4441 gtaatggata tcacgtaaga ttccggttct tgagatgggg gttcgattcc ctcagggtgg
     4501 atgtaaacat cctaaaaagc ctttaatagg gcttttttct ttacactgct ctaaatttgc
     4561 cgctaaaact gagaatctct gtctaaagaa ttcccaaatg ctttccagtc ccgttttaga
     4621 gtgactcagg gggctttttt tgatataata aaaaggactg ttatcagtta gaaagaggtt
     4681 ggtatgaaag aattacaaac tgtactaaag aaccattttg caatcgaatt tacagacaaa
     4741 aatttactgg aaactgcctt tactcatacg agttatgcca atgagcaccg cctcttaaaa
     4801 atttcacaca atgaacgctt ggaattttta ggagacgctg ttctacagtt attgatttca
     4861 gaatatctgt ataaaaaata tcctaaaaag cctgaaggtg acctatcaaa actccgtgct
     4921 atgattgtcc gtgaggagag tttagctggt tttgcgcgtg attgccagtt tgaccagttt
     4981 atcaagttgg gtaaagggga agaaaaatca ggtggtcgca atcgtgacac cattcttggt
     5041 gatgcctttg aagcctttct tggtgccctt cttttggata aggatgtggc caaggtcaag
     5101 gaatttatct atcaagtcat gattcctaag gttgaagcag gcgagtttga gatgattaca
     5161 gactataaaa cctatctcca agagttgctt caggtcaatg gtgatgtggc tattcgttat
     5221 caggtgattt ctgaaacagg gcctgctcac gataaggtct ttgatgtaga agttcttgtt
     5281 gaaggtaaga gcatcggtca aggccaaggt cgttctaaga aattagcaga gcaggaagct
     5341 gccaaaaatg ccgttgagaa ggggctggat tcatgtattt aaaggaaatc gaaattcagg
     5401 ggttcaagtc ttttgctgat aagaccaagg tcgtttttga ccaaggtgtg acggcagttg
     5461 ttggacccaa tggatctgga aagtccaata ttacagaaag tctgcgttgg gctttggggg
     5521 agtctagtgt caagagtctc cgtgggggca agatgccgga tgtcatcttt gctggaaccg
     5581 aaagtcgcaa accgctcaat tatgcttctg tagttgtgac tctggataat catgacggat
     5641 ttatcaagga tgcaggtcaa gaaatcaggg tggaacgcca tatctatcgt agtggagata
     5701 gcgaatacaa gattgacggc aagaaagtcc gtctgcgtga tattcatgac ctcttcttgg
     5761 atactggatt gggacgagat tccttctcta ttatttccca agggaaggtt gaggagattt
     5821 ttaattccaa gtctgaggaa cgacgagcta tttttgaaga agctgctgga gttttaaaat
     5881 acaagactcg cagaaaagaa accgagagta aactgcagca aactcaggat aatctggacc
     5941 gcttagagga cattatctac gagttggata atcaaatcaa gcctcttgag aagcaagctg
     6001 agaatgcccg taagttttta gacttggaag gacaacgtaa ggctatttat ttagacgttc
     6061 tggttgctca aatcaaggaa aataaggcag aactagagtc gacagaagaa gagctggctc
     6121 aggttcaaga actcttgatg agttattacc aaaagcgtga aaaattagaa gaagaaaatc
     6181 aaactcttaa aaagcaacgc caagatttac aggatgaaat ggccaaagac caaggcagtt
     6241 tgatggactt aactagtctg attagtgatt tagaaagaaa attagcccta tcgaaactgg
     6301 agtccgagca agtggccttg aatcaacagg aggcacaagc tcgtttggct gctttggagg
     6361 ataagagaaa ttcactcagc aaagaaaagt atgataaaga aagctcttta gctctgttag
     6421 agggaaatct agtccaaaat aatcaaaaac tcaatcgttt agaagctgaa ttgctggctt
     6481 tctcagacga tcctgatcag atgattgagc tcttacgtga acgctttgta gctcttttac
     6541 aagaagaagc ggatgtctca aaccagttga cccgtattga gaatgagttg gaaaatagtc
     6601 gtcagctttc tcaaaaacaa gcagatcaac tagaaaagct gaaagagcaa ttggctatag
     6661 ctaaagagaa ggctagtcag caaaaggaag agcttgaaac tgccaaggag caggttcaga
     6721 aattattggc tgactaccaa gctattgcca aggagcaaga ggagcagaaa acttcctatc
     6781 aagctcaaca aagccaactc tttgaccgtc tggataatct caaaaacaag caggccagag
     6841 ctcaaagttt ggaaaatatc ctgagaaatc atagtaactt ttatgcaggt gttaagagtg
     6901 ttctccaaga aaaagatcgc ctaggtggga ttattggtgc agtcagtgag catctgacct
     6961 ttgatgttta ttatcaaact gccctagaga ttgccttagg ggcaagtagc cagcatatca
     7021 tcgtagaaga tgaagagtcg gcaaccaagg ctattgactt ccttaaacgg aacagagccg
     7081 gtcgtgcaac ctttcttcct ttaaccacta tcaaggcgcg tacgatttct agtcagaacc
     7141 aagatgctat cgctgtaagc ccaggtttcc ttgggatggc agatgagttg gtgacttttg
     7201 atactagact ggaagccatt ttcaagaact tgctagctac gacggctatc tttgataccg
     7261 tagaacatgc gcgtgcagca gctcgccaag ttcgttatca ggttcgtatg gtgacattgg
     7321 atgggacaga attacgcacg ggtggttctt acgcaggtgg agccaatcgt cagaacaaca
     7381 gtattttcat caagccagaa ctggagcaat tacaaaaaga aattgctgaa gaagaagcaa
     7441 gtttgcgttc agatgaagtg agtttgaaga acttgcaaga tgagctagct agattgacag
     7501 aaagattaga agccatcaaa tctcagggag agcaggctcg tattcaggag caaggcttgt
     7561 ccctcgccta tcagcagacc agtcagcaag ttgaagaact ggaaactctt tggaaactcc
     7621 aagaagagga aatagatcgt ctttccgagg gagattggca agcggataag gaaaaatgcc
     7681 aagagcgtct tgctgcaatc gccagtgaca agcaaaatct ggaagctgag attgaagaga
     7741 ttaagtctaa caaaaatgcc atccaagaac gctatcaaaa cttgcaggaa gagctagcgc
     7801 aagctcgttt gcttaagaca gaactgcaag ggcaaaaacg ttatgaagtt gctgatattg
     7861 aacgcttagg caaggaattg gacaatcttg attttgaaca agaagaaatc cagcgccttc
     7921 ttcaagaaaa ggttgataat cttgagaagg ttgatacaga attgctcagt caacaggcgg
     7981 aagaatccaa aactcagaaa acaaatctcc aacaaggttt gattcgccag cagtttgagt
     8041 tggatgatat agaaggtcaa ctggatgata ttgccagtca tttggatcag gctcgccagc
     8101 agaatgagga gtggattcgc aagcaaacac gtgctgaagc caagaaagaa aaggtcagcg
     8161 aacgcttgcg ccatctacaa agtcaattaa cagaccagta ccagattagc tatactgaag
     8221 ctctagaaaa agcgcatgaa ttggaaaacc tcaatctggc agagcaagaa gttaaggatt
     8281 tagagaaggc tattcgctca ctgggtcctg tcaatataga agctattgac cagtacgaag
     8341 aagttcacaa ccgtctggac tttctaaata gtcagcgaga tgatattttg tcagcgaaaa
     8401 atctgctcct tgaaaccatt acagagatga atgatgaggt taaggaacgc tttaaatcaa
     8461 cctttgaggc cattcgtgag tcctttaaag tgaccttcaa gcagatgttt ggcggaggtc
     8521 aggcagactt gatattgact gagggcgacc ttttaacagc tggtgtggag atttctgttc
     8581 aacctccagg taagaaaatc cagtcgctta acctcatgag tggtggtgaa aaagctctat
     8641 cggctcttgc cttgctcttc tccattattc gtgtcaagac tatcccattt gtcatcttgg
     8701 atgaggtaga ggctgcgctg gacgaagcca atgttaaacg ttttggggat tacctcaacc
     8761 gctttgacaa ggacagccag ttcatcgtcg taacccatcg taagggaacc atggcagcgg
     8821 ctgattctat ctatggagtg accatgcaag aatcgggggt ctcaaaaatt gtttcggtta
     8881 agttaaaaga tttagaaagt attgaaggat gacaattaaa ctagtagcaa cggatatgga
     8941 cggaaccttc ctagatggga atgggcgctt tgatatggat cgcctcaagt ctctcttggc
     9001 ttcctataag gaaaaaggga tttactttgc ggtggcttcg ggtcggggat ttctgtctct
     9061 ggaaaaatta tttgctggtg ttcgtgatga cattattttc atcgcgggaa atggcagttt
     9121 ggtagagtat caaggtcagg acttgtatga agcgactatg tctcgtgact tttatctggc
     9181 aacttttgaa aagctgaaaa cgtcacctta tgtagatatc aataaactgc tcttgacggg
     9241 taagaagggt tcatatgttc tagatacggt tgatgagacc tatttgaaag tgagtcagca
     9301 ttataatgaa aatatccaaa aagtagcgag tttggaagat atcacagatg acattttcaa
     9361 atttacaacc aacttcacag aagaaacgct agaagctggt gaagcttggg tcaatgataa
     9421 tgtccctggt gtcaaggcta tgacaactgg ctttgaatct attgatattg ttctggacta
     9481 tgtcgataag ggtgtagcta ttgttgaatt agctaaaaaa cttggtatca caatggatca
     9541 ggtcatggct tttggagaca atcttaatga cttacatatg atgcaggttg tgggacatcc
     9601 tgtagctcct gaaaatgcac gacctgaaat tttagaatta gcaaagactg tgattggtca
     9661 ccataaggaa cggtcggtta tagcttatat ggagggctta taatggcaga tataaaattg
     9721 attgcattgg acttggacgg gaccttgctg actactgata aaaggctgac ggatcgtacc
     9781 aaggaaacct tgaaagctgc gcgtgatcgt ggtatcaagg tcgtattgac aactggtcgt
     9841 cccttaaaag ctatggattt ctttctccat gagctaggga ctgacggtca ggaagatgag
     9901 tataccatta cttttaatgg tggtttggtt cagaaaaata caggtgaaat ccttgataag
     9961 acagtctttt catatgatga tgtggcacgc ttgtatgaga aaacagagaa attatcactg
    10021 ccacttgatg ccatctcaga aggaacagtt tatcaaatcc aatcggacca agaaagtctt
    10081 tatgccaaat tcaatccagc tttgactttt gttccagttg actttgaaga cctgtctagt
    10141 caaatgacct acaacaaatg cgtgactgcc tttgctcaag aacccttgga tgcagccatt
    10201 cagaagattt ctccagaatt gtttgaccaa tatgaaatct ttaaatcacg tgaaatgttg
    10261 ctagagtggt caccaaagaa tgttcataaa gcaacaggtt tggcaaaact aatcagccat
    10321 ctcggaatca accaaagcca agttatggcc tgtggtgacg aggccaatga cctttctatg
    10381 attgaatggg caggtcttgg tgttgctatg caaaacgctg ttcctgaagt aaaggcagcc
    10441 gcaaatgtag taatgccgat gaccaacgat gaggaagctg tcgcctgggc tatcgaagaa
    10501 tatgtgctaa aggagaacta agatatggga ttgtttgacc gtctattcgg aaaaaaagaa
    10561 gaacctaaaa tcgaagaagt tgtaaaagaa gctctggaaa atcttgattt gtctgaagat
    10621 gttgatccta ccttcacaga agttgaggaa gttcctcagg aagaagcaga ggttgaaatt
    10681 gttgaacaag ctgtgttcca agaggaggaa atccaagaca cagttgaaga aagtcaggat
    10741 tcagagccag ctgtaggggt ttctcaagaa gaaatagaag aattaccaaa ctcagaagaa
    10801 ttaccagagg aagagaagct tgagcacgaa ggaactgtag aagaaaataa ttctgaagtt
    10861 cttgaaccag aaaggcctca aacagaagaa actgttcagg aaaaatatga ccgcagtctc
    10921 aagaagactc gtacgggctt cggtgctcgc ttgaacgcct tctttgctaa cttccgctct
    10981 gttgacgaag aatttttcga ggaactggaa gaactgctga ttatgagtga tgttggtgtc
    11041 caagtcgctt ctaacttaac ggaggaacta cgttacgaag ccaagcttga aaatgccaag
    11101 aaacctgatg cacttcgtcg tgtcatcatt gagaaattgg ttgagcttta tgaaaaggat
    11161 ggtagctacg atgaaagcat ccacttccaa gataacttga cagttatgct ctttgttggt
    11221 gtgaatggtg ttgggaaaac aacttctatt ggaaaactag cccaccgcta caaacgaact
    11281 ggtaagaagg tcatgctggt tgcagcagat accttccgtg cgggtgcagt agctcagcta
    11341 gctgaatggg gccgacgagt agatgttcca gtagtaactg gacctgaaaa agctgatcca
    11401 gccagcgtgg tctttgatgg tatggaacgt gccgtggctg aaggtatcga tattctcatg
    11461 attgatactg ctggtcgtct gcaaaataag gataacctta tggctgagtt ggaaaagatt
    11521 ggtcgtatta tcaaacgtgt tgtgccagaa gcaccacatg aaaccttctt ggcacttgat
    11581 gcatcaacag gtcaaaatgc cctagtacag gccaaagaat tttcgaaaat cacaccttta
    11641 acgggaattg ttttgactaa gattgatgga actgctcgag gaggtgtggt tctagccatt
    11701 cgtgaagaac tcaatattcc tgtaaaattg attggttttg gtgaaaaaat cgatgatatt
    11761 ggagagttta actcagaaaa ctttatgaaa ggtctcttgg aaggcttaat ctaatcagaa
    11821 gcaaaaatcc tgcaaggtac aaacttgcag gattttttta ttcgaaacga ccatcttgac
    11881 gataggcgat atctggttgc caagtccatt tggcatcgaa tttttcaagt aggtcaaagc
    11941 tggcttgagg tcccatgctt ccagctttat agtcatgaag tggggcacca ttttcggccc
    12001 agagctcttc aatacggtca atcaacttcc atgacgcacc aacctcatcc cagtggctaa
    12061 agttagttga gttgttattt aggacatcat aaatcaattt ctcgtatggt tctggagaag
    12121 caccagttgc agtcgcgtct gtacgataat caagtgagtt aggagccaag ttaaattctt
    12181 ctcctacttg cttcccattt aggctaagag agaagccttc tgttggttga atatagatgg
    12241 tcaaaatatt tggagcaagt ggttctccaa agatagaatc catttgttta aagacgatgt
    12301 tgacatgagt tcctttttca gtcagtcgtt tacctgtacg gaaaaagaaa ggaacaccac
    12361 ggaatcgatc gctgtctaca aagaaggcac cagatgtaaa ggtttcagtt gttgattctg
    12421 gattcacatt tggttcgcta cgataagaga tgtatttcat gccatcaatc ttaccagagc
    12481 ggtattgtcc acggataaag tgttctttga gttcttcatc agttggatga tagaggtttt
    12541 taaagacctt aatcttttca gcacgaatct cgtcttttgt gaagcttgct ggtttgtcca
    12601 tggcgaggag cgaaagaagt tgtagagtgt ggttttggac catgtcacgg agggcaccgg
    12661 attggtcata gtagccacca cgttcttcta cacccaagcg ctccgcaaag gtaatttgaa
    12721 cattgtcgat aaaatccttg ttccaaacgt tttcaaaaat caagtttgca aagcgaactg
    12781 caaagatgct ttggatcatt tccttgccaa gataatggtc gatacggaaa atttgttctt
    12841 cgtcaaatgt tgctaggagt tcgtcattca acttgcttgc agttgcgtaa tctgtaccaa
    12901 atggtttttc aacgatcaag cgctcaaaac ctttgccatc gacaatgttt tcagatttga
    12961 ggtgtttggc aatggttcca aagaactgag gtgccataga caagaagaag agcttattgt
    13021 gttcagcttg gtatttttca ttaagctcag cttgtaattg acgcaaagca atataatgtt
    13081 ccgaatcatt gacatcatgg ctttgatagt agaagtggct agcaaattct tgggcttgct
    13141 cggtactatc tgccaaatca aggatggact cgacaactac agattcaaaa tattccttac
    13201 tccaaggtct acgggcagtt ccaataacgg caaagtgctt ggaaagattg ccggatttat
    13261 atagtctaaa aagggaaggg tagagcttgc gtttagccag gtctccactc gcaccgaaaa
    13321 ttgtaacaat aaccttagat gacatctagc tacctaattt ctatttttat tcctagtctt
    13381 gctaggtatg aggaaacctc atttcataaa cttaattcta acataatttt ccgaaaaatc
    13441 aaaaattcca atacgaggta gaaaaactgc aaggaaatcc ttacagtttg agtttagacg
    13501 tttaagacct tatctaagaa ctctttcaga cgagggtgtt gtgggttatc aaagatttgg
    13561 tcaggtgttc cgtcttcaag gaactcgcca tctgcagtaa agataacgcg gttggcaacc
    13621 tgacgagcaa atcccatctc atgggttacg ataatcatgg tcatgccttg ctcagccaat
    13681 tccttcataa cgttaagtac gtctccaacc atctcagggt caagggcaga agttggttca
    13741 tcgaagagca tgatgtctgg attcattgct aggccacgag cgatggccac acgttgtttt
    13801 tgaccacctg ataggctatc tggattggca ttagctttat ctgctagtcc aaccttttca
    13861 agcaactcca ttcccaattc ctcagcttct tccttagtca tcaacttgtg ctcaatagga
    13921 gcaaaggtga tgttgtccaa tacagacata tgagggaaga ggttgaagtg ttggaatacc
    13981 atgccgatat tttcacggac gtggtcaaca ttggttgttt tttcagttaa atcatagccg
    14041 ttcacagtga tgtgaccgct agtgacttct tctaaaagat tgaggctacg gaggaaagtt
    14101 gacttaccag aacctgaagg accgatgata caaacaacat ctccttcata gaacttagtc
    14161 gtaattcctt ttaggacttc attttttcca tagtgcttgt gtaaatcatt tacatcaatt
    14221 tttaattttg ccattaacga atcctctttt ctaagcgttt cgctagtcta gtcaaaagtg
    14281 tgataattac aagatagaag atagcaagga ttgcatacat cttgaaactt tggtagttac
    14341 gagcaatgat aatcttacca gtttggaaga gttcaaccaa accgatagca gatacgatag
    14401 ttgtatcttt aagagcgata acgaattggt tgacaaagtt tggcaacatc aatttagttg
    14461 cttgtggcaa gataatctta cgcatggttt ttccataaga gatacccaag cttcggctgg
    14521 cttccatttg gccaactgga acggcctgaa taccaccacg aacgatttca gcgatataag
    14581 cagccgcatt gagtgagagg gcaattgttc cagctacaaa gtcgttaatt gggctttgtt
    14641 ggcctgtgat agactcgatg aagtttggaa ttccccagaa gatgaaggct gcaagaatca
    14701 tcaatggaat accacgaata acgtcaacga aaatctcaga gatgacgcga agagatttgt
    14761 atgggctaac gctaaacata ccgaagataa ttccgatgac aatggcaata gcaaatgaga
    14821 taagagctag agcaagagtg ataccaagac cgctaaggag ttgtttgtaa ttgttttgaa
    14881 gcaagcccca gagcgttgtt tcgtcaacag tacttgttga agcagttgaa gattcgctag
    14941 ctaggtattt gtcaagaatc ttttggaatt caccgtttgc tttaaggttt gcaagtccgt
    15001 tgttgaacat ttcaatcagt tctggatttg ctcctttttt aacggcaaag gctgtttcac
    15061 cgattggagt tccagagatt ggagttttca atttttgacc ttggctgata gaatatttga
    15121 gaacaggttc atcatccata acggcatcaa tggcaccagt atttaaactg tcatacattg
    15181 aagaaccatc agcaaaggtt ttgattttgt agccgtattt gctttgattt tctgttagga
    15241 aggtttgaga agcagttccg tttttaacac cgactgtctt tccttttaga tcttcataag
    15301 aagcaatatt gcttgattct ttgacaccaa gaatggtatt agcagtgtag tatgattctg
    15361 agaagtcaaa agttgcctta cgagcatctg tgacagacat accagcgatg ataccatcgg
    15421 cttgaccagc ttggacagca ctgatagcag catcaaaacc agggttggtg atttcaattt
    15481 caaaaccttg gtctttagcg attgccttaa tcaattccat atcaatacca gtgtattggt
    15541 tgcttgaatt ttggaaaaca aaaggtgcaa aagaagaatc gctggcaatg atatatttag
    15601 ccttaacagg aatggctttt aatcctgcga gagtagttgt agttggcact gctgaagatg
    15661 atgaagcagt ccatttcttg ataattttat caagactacc atcttttttc atttcagaca
    15721 aggcttggtt aaattcagta accaggtgct cgtatttact tccttttttc acaccgaaag
    15781 caaaacttcc tacagcttca ccatccattt caatatggag gtcttgacct tggttaatgg
    15841 catattcgat aacaggtttg tcatccatca tggcatcgat ggcaccagca ctcaagctgt
    15901 tgttcattaa atcaccagtg tcaaatgttt taatagtaaa gccgtattta tctttgattg
    15961 tttcaaggaa acgttgagcg gcagttccgt ttttaacacc aacggttttg ccagttaatt
    16021 ggtcgtactt gctaattttg tgtgactttg tagtagcaat gacaactttt gtatcatagt
    16081 aagtatcaga catggtgaag actttttcac gttctttagt ctttgtcatc cctgccatga
    16141 tagcgtcggc ttgcccagct tgaaccgcat tgactgctgc gtcaaatcca ggataggaca
    16201 tctgaatgtt ccagccttta atctcagcga ctttgttaat aatgtcaaca tcaattcctt
    16261 tataagtttg atctgaatct ttaaactcaa aaggtgcata ggcggtatca gaaacaatct
    16321 taatcgtttc tgctttggca atacctaatg agaaaattgg gaataaaatt agcaaaaatg
    16381 ctagaaattt tttcttcatt tttagtctcc ttttccgaat attctctcat tatatcagaa
    16441 aaagtaaaaa aaggcaaatt tttatatttt tcgtgtcaga aaatataaca tcg
//
LOCUS       DAVLWA010000038        15729 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLWA010000038 DAVLWA010000000
VERSION     DAVLWA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.038"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            112..3213
                     /locus_tag="VYN80_001188"
     CDS             112..3213
                     /locus_tag="VYN80_001188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1541104.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3296..4303
                     /gene="pfkA"
                     /locus_tag="VYN80_001189"
     CDS             3296..4303
                     /gene="pfkA"
                     /locus_tag="VYN80_001189"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1541105.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTVMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4362..5867
                     /gene="pyk"
                     /locus_tag="VYN80_001190"
     CDS             4362..5867
                     /gene="pyk"
                     /locus_tag="VYN80_001190"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1541106.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6602..6827)
                     /locus_tag="VYN80_001191"
                     /pseudo
     CDS             complement(6602..6827)
                     /locus_tag="VYN80_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7675..8547
                     /locus_tag="VYN80_001192"
     CDS             7675..8547
                     /locus_tag="VYN80_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1541107.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8693..9037)
                     /locus_tag="VYN80_001193"
                     /pseudo
     CDS             complement(8693..9037)
                     /locus_tag="VYN80_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9180..9491
                     /locus_tag="VYN80_001194"
     CDS             9180..9491
                     /locus_tag="VYN80_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541108.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            9495..10211
                     /locus_tag="VYN80_001195"
                     /pseudo
     CDS             9495..10211
                     /locus_tag="VYN80_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10341..11156)
                     /locus_tag="VYN80_001196"
     CDS             complement(10341..11156)
                     /locus_tag="VYN80_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1541109.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11153..12058)
                     /locus_tag="VYN80_001197"
     CDS             complement(11153..12058)
                     /locus_tag="VYN80_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1541110.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(12388..12471)
                     /locus_tag="VYN80_001198"
                     /pseudo
     CDS             complement(12388..12471)
                     /locus_tag="VYN80_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12515..14644
                     /locus_tag="VYN80_001199"
     CDS             12515..14644
                     /locus_tag="VYN80_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1541111.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14631..15080
                     /locus_tag="VYN80_001200"
     CDS             14631..15080
                     /locus_tag="VYN80_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1541112.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            15139..15411
                     /locus_tag="VYN80_001201"
     CDS             15139..15411
                     /locus_tag="VYN80_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1541113.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15542..>15729)
                     /locus_tag="VYN80_001202"
     CDS             complement(15542..>15729)
                     /locus_tag="VYN80_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541114.1"
                     /translation="EKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIF
                     IALNIKKERTKFVLSRA"
BASE COUNT         4840 a         2797 c         3393 g         4699 t
ORIGIN      
        1 atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag agaagccatt
       61 tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa attgatcgca
      121 caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat cgaaaagtat
      181 gtgagagcag ctaaagaata cggctacact catttggcta tgatggatat tgacaatctt
      241 tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc tttgataggg
      301 cttgaaatga cagtgtttgt agatgatcag gaagtgaatt tgcgcttttt agctctatct
      361 agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg ggagaaaact
      421 tggtcagtcc tgtcccagta cctggaggat atcgcggtca ttgtgcctta ttttgataga
      481 gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga aacactagca
      541 agcgaatttc atcatcctat cttacctctt tatcgggtca acgcttttga aagcagggat
      601 agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag agaagttccc
      661 ttgcgttcga gacaagatgt ctttatatca gcaagttctt tagagaaact attccaagag
      721 tgttttccgc aagctttgga caatttagaa aagcttattt caggcatttc ttacgacttg
      781 gatactagtc tgaaactgcc tcgttttaat ccagctagac cagcagtaga ggagttgaga
      841 gagcgtgctg aactggggct tgttcagaag gggttgacta gtaaagaata tcaagataga
      901 ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt cttggttgtt
      961 tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg aaggggttct
     1021 gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc agtagagaaa
     1081 aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgcctga tattgatatt
     1141 gatatcccag atatttatcg tccagatttt atcagatatg ttggtaataa atatggtagt
     1201 aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc tcttcgagat
     1261 gtcttgaaac gctttggtgt gccagagtat gaattatctg caattactaa gaaaatcagt
     1321 tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc agtttcgtca gcaaatcaat
     1381 agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg ctatccaaga
     1441 caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac caactatatt
     1501 cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg agttgaggct
     1561 agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt ccagaagatg
     1621 caagagttgc ttgctgaaat agaaggtatt caccttaaaa ttgaagaaat agatttggaa
     1681 gacaaagaaa cgttagcttt atttgcctct ggaaatacaa aaggtatctt tcaatttgag
     1741 caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag tctgttttga agatgtcgtc
     1801 gcgactactt ctctaaatcg accgggtgct agtgactata tcaataattt tgtggcaaga
     1861 aagcatgggc aggaagaagt gactgttctg gatccagtac tggaggatat tttggctcca
     1921 acctacggca taatgctcta tcaggagcag gttatgcagg ttgcccagcg atttgccgga
     1981 tttagtcttg ggaaagccga tattttgcgt cgggctatgg ggaaaaagga tgcctctgcc
     2041 atgcatgaga tgagggcttc ctttattcaa ggttcattag aagctggtca tactgtggaa
     2101 aaagcagagc aggtctttga tgttatggag aagtttgcag gttatggttt taacaggtca
     2161 cacgcctatg cctactcagc cttggccttc cagttggctt attttaaaac acattatcca
     2221 gccatttttt atcaggtcat gttaaattct tccaacagtg attacttaat agatgcactt
     2281 gaagcaggtt ttgaagtagc ctctctatcc atcaacacca ttccctatca cgataaaatt
     2341 gccaacaagg ccatctatct aggtttgaaa tccattaaag gagtcagtaa tgatttagct
     2401 ctctggatta ttgaaaatag accttattct aacattgaag attttatagc taaattacct
     2461 gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct tttcgattca
     2521 tttgaaaaaa atcgtcaaaa agtatttaat aatttagcta atctatttga atttgtgaaa
     2581 gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga agattggacg
     2641 gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag caaacatcca
     2701 ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa tttgtcagaa
     2761 aatagctatg ctattatctt ggttgaagtt cagaaaataa aagtgattcg taccaaaaag
     2821 ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga tgtcactctc
     2881 ttttcagact tatatcgtca ggttggacag gaaataaaag agggagcctt ctactatgta
     2941 aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga ttgcacaaga aataagagaa
     3001 gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca agaaatttca
     3061 cgcattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga agaggaacag
     3121 aaaaccatcg tttctcccca tcattttgta gctaaatcca atgaattaga ggagaaattg
     3181 aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa gaattttaaa
     3241 atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa ccaatatgaa
     3301 acgtattgct gttttgacta gtggcggaga cgcacctggt atgaacgctg ccatccgtgc
     3361 agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg acggatatgc
     3421 tggtatggtt gccggtgaaa ttcatcccct agatgcagct tcagtagggg acatcatttc
     3481 tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac ttgaagggca
     3541 acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg ttatcggtgg
     3601 tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag ctattggtct
     3661 tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt ttgacacagc
     3721 ggttactact gtcatggacg ctatcgataa gattcgtgat acatcatcaa gtcaccgtcg
     3781 tacttttgta atcgaagtta tgggacgtaa cgctggtgat atcgctcttt gggctggtat
     3841 tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg aagatatcgt
     3901 agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg tcttagctga
     3961 aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag atacaagcga
     4021 ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg cgcgtgaccg
     4081 tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag gtatcggtgg
     4141 tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg gtactgcaga
     4201 agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca acccgcacaa
     4261 agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa tttagttaat
     4321 aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa cgtgtaaaga
     4381 tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc ggtgaggacg
     4441 gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct aaattgattg
     4501 aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa gaacaaggtg
     4561 agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt ggtttccttc
     4621 ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct aaagaatatt
     4681 catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa tcaactcgtg
     4741 aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt gaagttggtc
     4801 gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa gatgatgcaa
     4861 ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa aaaggtgtga
     4921 acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac gacgatatcc
     4981 gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt actgcaaaag
     5041 atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt caattgttcg
     5101 ctaaaatcga aaaccaacaa ggtatcgata acttagatga aatcatcgaa gcagctgatg
     5161 gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg gttccagttt
     5221 atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc actgcaacaa
     5281 acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta tcagacgtat
     5341 tcaacgctgt tatcgacgga actgacgcta caatgttgtc aggcgagtct gcaaacggta
     5401 aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct caagctcttc
     5461 ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag acagaagtaa
     5521 tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt gtaactctta
     5581 ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct gacatcttag
     5641 cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt gttatcccaa
     5701 tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa cgtaaagcgg
     5761 tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt gtgccagtag
     5821 gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa aaatataaaa
     5881 acctatcata tccagctttc tagcctgtat gatgggctct ttttttgtcc agggggcaaa
     5941 aagggggcaa aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc
     6001 agcgtcgcct taccgtatat atgttactga cttcgtcagt tctatctaca acctcaaagc
     6061 agtgctttga gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg
     6121 aaataagata tgcataaatt gattagaaag tcaaatgaat ttctacaaat gttttagcaa
     6181 tcgcaatcta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
     6241 aaacctccaa agtagcttgc tccattcttt tacctacgtg agtctagatg ttatttactg
     6301 ttttagcgtt tttgtgttct actctaacca ttatagcatt cttctcagct agtgtactaa
     6361 ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag
     6421 tatttgcctt ttgcaaagtg atcttaaatg cctttctcta aatttacata tcactattgt
     6481 ttaacaaaat ctaatctatt ttaggtcact tattcttttt ttgaaatgta gaatgaactt
     6541 tttcaaagtt tttcgaatct tttaaaatct gtttgtttta tatcgccatt ctcccccttt
     6601 tttaattctc cctatatagc ctgacaactt ttccgatggt acgaatatgg ttgctttcgt
     6661 ctaggtggat gtcggagtat tcgggattga gtttttttga ggtagccttg gcggagtttc
     6721 ttgacatagt tagtgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg
     6781 gtattcttga taaataggta gtcgctgttt cttatctttg gctccatgga cttgctgacg
     6841 acataagcga ttgggtcgta gtcgtctggg ataatggaaa ctccataact aaatcgtcgt
     6901 cctgcatcaa gcggctacct gcagagataa actacctaac acgagagtaa gtagtctgtc
     6961 tgtagtcgtc cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct
     7021 ctcggcatag gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg
     7081 gatatcgcta ggagaatcct tttgaactgg aggaaagagg gcatcaatca agctactgaa
     7141 tactttaact aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt
     7201 tctaatggtg tttttactta tacctatctt agtacccaat tcttattgag tccaaccatt
     7261 actagtctat attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa
     7321 catttctagg aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctac
     7381 tatatttttc acctctaaca atcgcaatct cttctttatc catgaacgaa aacgctttct
     7441 atctttgtaa gtaaagcatc acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc
     7501 gtaaaaaatt tgttgacaga gcacgaaacg cctactataa ttaaatcaag tttaaggaaa
     7561 taacaaaaaa acaaatcgga gggaaagacg atgaacacat taaatgagaa agtaatcaat
     7621 atctgtaaag cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
     7681 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
     7741 tgtcaacaac aacatgctac ttctgagggg acgaatcaaa ggcaaagcag ttcagcgaaa
     7801 gttccatgga aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa
     7861 tctctcttat cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat
     7921 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
     7981 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgatttcgtt
     8041 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
     8101 cttgaaaaga atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc
     8161 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgaggca
     8221 actacagatg tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc
     8281 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atctggatgg cgagtatctg
     8341 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     8401 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     8461 tatttaggca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     8521 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     8581 ttatttctat ttttgatgag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     8641 aaaaatgaaa catttattgg ctatatccaa agatacgata ttgatgggaa tggctatcaa
     8701 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaaaacta ctttgaacaa
     8761 gagttacttc aacccattgg ctccgacgga ttaagttgct ttcgtgaaca ccaaaatcag
     8821 ccgcaatttc ttcataagtg cggtattctc gcacatattg aagagtggcc ataagaaggt
     8881 cttctaggct taatttaggt tttcgtcccc cttttgtgtg tttaagttga taagcagttt
     8941 ttaatacagc taacatctct tcaaaagtgg tgcgctgaac accaacaaga catttaaatc
     9001 gtgcatcagt tagttgctta cttgcttcat aattcataga actactatac catattttgt
     9061 ttcgcaggaa gtctaatgct aaaatcttat cagtgagaag atttcttaaa gtttattctt
     9121 tgtagtttca tctgtattag tacataattt aacttagaga aaataggagg actaatcgaa
     9181 tggaattaaa agattttaca gaaaaagaac aggaaatgat taagaaaagg cttacaatgt
     9241 ctaatattag tgacaaagaa actactgaga agattctggc gctagtacca caagacttga
     9301 ttaagcgaat cccgtttttt gtcagaaaac atgctacaac acgtacgatt aaacgcattt
     9361 caattgaaca ccctgaactc tacgctgtag ctcaaacaag tggtgagatt ccagaaaaaa
     9421 aacgcgaaga attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata
     9481 gtattaagta gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata
     9541 gcaaggattt tctttaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc
     9601 atcttcgaga tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat
     9661 ctatctgtct gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat
     9721 ttgaagaagt aaagaaactg attcttttgg atggtggcta tctagattta gataagattt
     9781 tacctttgga tacagagtta gaggaaacta aaaattatat caaatctcaa attatttcgg
     9841 acttagatgt tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg
     9901 agaaagctgt aagacagtcc tatcactgga atgttgagta taatagatat gagttggcta
     9961 taaattatga aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga
    10021 gagaagtggg agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag
    10081 aggaagccct aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc
    10141 atgagcttta tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt
    10201 attttctatg agctacttgt attgctggaa atggtataag atgaaaatct acaagtcact
    10261 aattttcata taaccaaaaa acgaacaggc tctttttctg ttattcagaa gagaacttgt
    10321 tcgtttttat cttgattttc ttaaaaagct ctatatacat agggattttc tggtttatcg
    10381 actttggtaa agctgtcgat ttccaaggtt ggaaggtttt gtttgagttc tttatggtag
    10441 tgattgacca gttttccttt ggctgaaatc caagtgttgt tttcatactg ccgggtattg
    10501 cccttggtca gcaatccata gacaccagaa tctgcgatac agtggataat gccgaatcgg
    10561 aacagaaatt gactattggc atgactgggg tcgttataga caaagcctgt aaactggatt
    10621 gtcttgccct caaactcatc tggatagtcg tagatagcct ccatgacttc catatagttt
    10681 tcattagtga tctgaatgct gtcttgggat aagtatttat ccgccgccgt tcgcatttcc
    10741 ttttcatagg ctgattttga aaaataagaa ctagtatctg gtttcaaata ttggcttgtc
    10801 gtcccttcgc ttgtctgaat agccagattc gttccttccg atagagggaa atgatagcct
    10861 ttagctgaaa cagtctgaga atccaagcta acagttggga aagttaagcc gatgacaagt
    10921 ggaatagcca gaagagaaat actcgtcatc ttggccaatc ggctgttcag atgactgtgg
    10981 gttttgactt gcttcatcca gatatacaat tgaacgatag ccaagataaa agaaagcacc
    11041 atggagatat aggccagata ggaatagtgc atgttgatgt actggtttag tttgcccgac
    11101 agatggaggt aaatagtcag ttcaaaatag ccagctaaaa ctaaaaatcg aatcatagga
    11161 tgactccaat caagagagaa tagactaaga cgacaagagt tacaattgtt atgaagtgac
    11221 tgataaatcg tgctttcaag taatttttca tcatgagaat atttttgata tccagcattg
    11281 gaccaatgac gagaaaggcc agaactggtg ccaaaccgaa actcgagaga agagaagcac
    11341 ctataaaggc atccgcctca ctacagagcg aaagaagaaa ggctaaaatc atcaagagca
    11401 ggatggcaaa aagaggggtc gcactgatag aggtcagaat ccgagtcgga acgtagacct
    11461 gtattataga agcaaagagg cagccaaata ccaaataacg ccccgtatca aaaaattcat
    11521 caatggcctg cacaaagact tgaaaaactt tttttgcaga actcaagtaa gaaaaatcat
    11581 gctcatgaca agccagacga ttttctttct gaatcggttc ttgccagaaa aatcctagaa
    11641 aaattcctag tattacagcc acaagaatgg aacccagagc tcgtaatagg gcgacatgga
    11701 aggagttgcc aaaggcagaa taggtcgcaa aaagaacaat gggattgata acaggtgctg
    11761 tcacaagaaa aggaacggcc gtgtaacttg gaaccttttt ttccagaaaa cgattgatga
    11821 tggggacgat tccacattca caagaaggga aaagtatacc gacaaaggtc ccaaaaaaga
    11881 ttctccccca acgatttcga gggagaaaat gataaacctt gtcaggtgtg atataaactt
    11941 caatcagccc tgagacaatg cttcctatca gaacgaaggg aagggcttca atgataatgg
    12001 agagaaaaat ggctccagtt tgaagtacac tagatgggag ttgttgaaaa gcagtcatct
    12061 agtttttctt ttctgctttg acttttttct ctttgtcatc agggaaagta acttgtttca
    12121 aatcagggag tttagcaaac tcttcaaaca tcttgtccaa gtcgtctcgt tttgtaaatt
    12181 tgacagccat aggtgtacct cgattctaaa ttctacctct attatactat tattttgcgg
    12241 aaaagggagt tttttaattt gttttcgata gttgatactc ttcgaaaatc tcttcaaacc
    12301 acgtcagctt cgccttaccg tagtacggtt actgacttcg tcagttctat ccacaacctc
    12361 aaaacagtgt tttgagctga cttcgtcagt cttatccaca acctcaaaac agtattttga
    12421 gcagcctacg gctagcttcc tagtttgctt tttgattttt attgagtatg aaaaggattg
    12481 gagaaagcct tccagcttgt ggtataatag atttatggat aaaaaatatg aaaaaatctc
    12541 tcaggatttg ggagtgacgt taaagcaaat tgataccgtt ctaagtttga cagctgaagg
    12601 ggcgactatt ccctttatcg cgcgttatcg caaggacatg actggtagtc tggatgaggt
    12661 ggcgattaag gctattattg atttggataa aagtctgaca aatctcaatg accgtaagga
    12721 agctgtctta gctaagattc aagaacaagg taagttgacc aaggaattgg aagaagctat
    12781 cttagttgcc gaaaaattag cagacgttga agaactctat cttccttata aggaaaagcg
    12841 tcgtaccaag gcaaccattg cccgtgaagc tggactcttt cctcttgctc gtttgatttt
    12901 gcagaatata gttgacttag agaaagaagc tgaaaagttc gtctgtgaag gatttgcgac
    12961 tggcaaggaa gccttgaccg gtgcagttga tattttggtc gaagccttat cggaagatgt
    13021 gaccttgcgt tctatgactt atcaggaagt gctgagacac tctaaactca cttctcaagc
    13081 caaggatgaa agtcttgatg aaaagcaggt ttttcagatt tattatgatt tttcagagac
    13141 agttggaact atgcaaggct atcgtacctt ggctctcaat cgtggggaga aacttggtgt
    13201 cttgaagatc ggttttgaac atgcgacgga ccgtattctt gccttctttg ctactcgttt
    13261 caaggtgaaa aatgcttata ttgatgaagt tgttcagcaa tccgttaaga aaaaggtctt
    13321 gcctgctatt gagcgtcgta ttcggacaga attaactgag aaagctgaag agggagctat
    13381 ccaacttttt tctgacaatc tgcgcaatct cctcttggtt gctccactga aagggcgcgt
    13441 ggttcttggg tttgacccag cctttcgtac aggtgccaag ctagctgtcg tggatgcaac
    13501 aggaaaaatg ctgacaactc aggttattta tcctgttaaa ccagcatcag ctcgtcaaat
    13561 cgaagaagcc aagaaagatt tagcagattt aattggtcaa tacggtgtag agattattgc
    13621 cattggaaat ggaacggcca gtcgtgaaag tgaagctttt gtagcggaag ttctgaaaga
    13681 tttccctgaa gtcagctatg ttatcgttaa tgaaagtggt gcttctgtct attctgccag
    13741 cgaacttgct cgtcaggagt ttccagactt gaccgttgaa aaacgctctg ccatttctat
    13801 cgcccgtcgt ttgcaagatc ctcttgcgga attggtcaaa atcgatccta agtcaattgg
    13861 tgtcggtcaa taccaacacg atgtcagtca gaagaaacta tctgagagtc tggactttgt
    13921 tgtcgataca gtggttaacc aagttggtgt caatgtcaat acagctagcc cagctcttct
    13981 ttcacacgta gctggactca ataaaaccat ttctgaaaat atcgtcaaat accgtgagga
    14041 agagggaaaa atcacttcac gctcccaaat caagaaagtt cctcgtttgg gggccaaggc
    14101 ttttgaacag gctgctggtt tcctccgtat ccctgaaagt agcaatatcc ttgataatac
    14161 aggagttcac ccagaaaatt acgctgccgt taaggaactc ttcaaatgct tggatattaa
    14221 ggatctgaat gaagaggccc aaagcaaact caagtcgctt tcagtcaagg aaatggcgca
    14281 agagttagac cttggtccag aaactcttaa agatatcatt gcagatcttc tcaaaccagg
    14341 tcgagatttc cgtgattcct ttgatgctcc agttctccgt caagatgtcc tagatatcaa
    14401 ggacttagtg gttggacaaa agctagaagg agtggtgcgt aacgttgttg atttcggtgc
    14461 ttttgttgat atcggaattc atgaggacgg tctcatccat atttcccata tgagtcgcaa
    14521 gtttatcaaa caccctagtc aagtggtggc agttggagat ttggtgagcg tttgggttaa
    14581 gaaaattgat actgaacgtg aaaaagttaa tctgtctctc ctagctccaa atgaaactga
    14641 ctgattacgt caagcaggtt tcactagaag acttcggcag accttttatc catcatgtcc
    14701 agtggaatag gcgtctacgt tcgacaggtg ggcgattttt ccccaaagat gggcatttgg
    14761 attttaatcc caaggtttat caggaactgg ggttggacgt ttttaggaaa attgtccgac
    14821 atgaactctg tcattatcac ctctattttc aagggaaagg ctatcaacac aaggatcggg
    14881 attttaagga actgttgaaa gcagtggatg gattacggtt tgtaccatcc ttgcccaata
    14941 gtaactccaa gccaatcaag ctctatcgtt gccaatcctg ccagcaaact tatcagcgta
    15001 agcgtaggat tgataccaaa cgctatcgct gtggactttg tcgaggaaag ttgctattag
    15061 taaatcagcc tgaggactga tgaaagccga gcctatccgt gatatactat gtctaaccta
    15121 gcagaaagag gaaatatcat gaacagtaag ttttataaaa tgagacgaaa tcgcatgatt
    15181 tcaggagttt tagcaggtct gtcagacaag tggaactttg atgttaccct tgtacgtttt
    15241 ctgtttgcca tttttacagt cgcaaacttt ggacttgggg tgattatcta tatcatccta
    15301 gcttctatca tgccaaccaa ggaagaaatc gaagcagaaa tgtacggaac aggcccacgt
    15361 aaacgcaaag aagctcaagc cattgacgat aatgaaggct ggttttggtg aggttttaca
    15421 aaagatttgg aatacgcaaa aagctttaaa tcaatagtct aaactgcttg atttaaagct
    15481 tttttacttt tatactcaat aaaaaacggc tctttgtcaa ctgtagtggg ttgaaaaaaa
    15541 gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattcaga gcgataaaaa
    15601 tccgtttttt gaagttttca aagttccgaa aaccaaaggc attgcgcttg ataagtttga
    15661 tgagattatt ggtcgcttcc aatttggcgt ttgaataggg tagttgaagg gcgttgacga
    15721 ttttctctt
//
LOCUS       DAVLWA010000039        15700 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLWA010000039 DAVLWA010000000
VERSION     DAVLWA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.039"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(23..2569)
                     /locus_tag="VYN80_001203"
     CDS             complement(23..2569)
                     /locus_tag="VYN80_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1541115.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2692..3132)
                     /locus_tag="VYN80_001204"
     CDS             complement(2692..3132)
                     /locus_tag="VYN80_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1541116.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3107..3976)
                     /locus_tag="VYN80_001205"
     CDS             complement(3107..3976)
                     /locus_tag="VYN80_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1541117.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(4018..4746)
                     /locus_tag="VYN80_001206"
     CDS             complement(4018..4746)
                     /locus_tag="VYN80_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541118.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(4754..5233)
                     /locus_tag="VYN80_001207"
     CDS             complement(4754..5233)
                     /locus_tag="VYN80_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1541119.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(5244..5942)
                     /locus_tag="VYN80_001208"
     CDS             complement(5244..5942)
                     /locus_tag="VYN80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1541120.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(5935..6201)
                     /locus_tag="VYN80_001209"
     CDS             complement(5935..6201)
                     /locus_tag="VYN80_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1541121.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(6267..7193)
                     /locus_tag="VYN80_001210"
     CDS             complement(6267..7193)
                     /locus_tag="VYN80_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1541122.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(7268..8083)
                     /locus_tag="VYN80_001211"
     CDS             complement(7268..8083)
                     /locus_tag="VYN80_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541123.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLAKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(8087..8407)
                     /locus_tag="VYN80_001212"
     CDS             complement(8087..8407)
                     /locus_tag="VYN80_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1541124.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(8693..10690)
                     /gene="metG"
                     /locus_tag="VYN80_001213"
     CDS             complement(8693..10690)
                     /gene="metG"
                     /locus_tag="VYN80_001213"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1541125.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(10814..12073)
                     /locus_tag="VYN80_001214"
     CDS             complement(10814..12073)
                     /locus_tag="VYN80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541126.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(12075..12776)
                     /locus_tag="VYN80_001215"
     CDS             complement(12075..12776)
                     /locus_tag="VYN80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541127.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(12760..13959)
                     /locus_tag="VYN80_001216"
     CDS             complement(12760..13959)
                     /locus_tag="VYN80_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1541128.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            14224..15570
                     /gene="gor"
                     /locus_tag="VYN80_001217"
     CDS             14224..15570
                     /gene="gor"
                     /locus_tag="VYN80_001217"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1541129.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         4652 a         3532 c         2812 g         4704 t
ORIGIN      
        1 tttcttttta ttttaggctt atttatgcat ttccgtattg aagaacaact gcttcgactg
       61 cagctttttc acggctaatc aagtcaacac gtgctgcaat ttccttgatt cccataccga
      121 tgttacggct aagagcaagg tcagaaagtt gcggctcaaa gaactccttg tattccgcca
      181 agcgttgctg agtcttaaat acatgagcag gaaggataac aaagctatca aagctcatat
      241 ctcctccaag ggctgcctta atccaagccc agttttcacg cgcccaagac caagctgttt
      301 tctgagttgc ttgatgagct aggaattggt aataccaagc agacaagtcc tgtggtttga
      361 ccacaaattt gtccttccaa gaagtaatca agttttggat attatccgca tctgtactgt
      421 atgcaagagc tgctgccaac tggcgtttaa agacagcatc tgttgcgtga gtataagtat
      481 caagataaag tgctaacaag tctttagtct catgatgttt catctcatta atcagaactt
      541 gtgaacgaat agctgctggg agttctgcaa gattctcctt gtgtgttgcg aagatttggc
      601 tagcgacttg acgagcttct gcatcatttg agcgaatcat catcgaaaca gccagctgac
      661 gaaccaattc atcctcatct gattctccgt ctttagcttc aaaaccaaga cggtcatagt
      721 tatgacgagc caatttagca accagtcctt tgaaggctgt ttcagcatcc gttccttcat
      781 caataaagcg ctcaagggct gaaatcactt gagaaacagc tgaaaccacc agataagact
      841 cttccttagc aagtttatca agaactggaa gcaagtctgc ataagaaatg tgccctgcct
      901 cagccaacaa acgacgttct tgaacaattt gcagtttgct tgtgttatca agtgtctcta
      961 gctcagcaag aacagcttct aacaagtctc cttgatagtc ggtaatatag tgggcagtat
     1021 tttcagtgtt gagacgaaga gctccttcat tttcagcaag aagagctgcg tagccaggga
     1081 tttcgatact ttcagtttcg agtgtatcag gcaagccttt ccagttgcta ttgaggggca
     1141 ccacccagag acggttcttg tcttcgttct caccgatgaa gaattgtttt tgtgaaatct
     1201 tcaagacatc attttcaact ttgacagtaa gaactgggta accaggctgt tccaaccaag
     1261 aatccatgaa ggctgcgaca tcacgtcctg acgcttgacc aagggcatcc caaaggtcac
     1321 taccaatggt gttgctgtat tggtgttttt caaagtaggc atgcaaacct ttagcaaaat
     1381 cagcatctcc tagccaacgg cgaagcatgt gcatgagacg gcttcctttg gcatagacga
     1441 tagcgccgtc aaagagtgta ttgatttcat ctggatgttt aacttcgacg tggacagact
     1501 gaacgccatc agtagcgtca cgttcaagag caagaggtac tccacctgtt tggaaatctt
     1561 caaagatatt ccagcttggt tcgatggtat ccacacagac gtattccatc atattagcga
     1621 aactttcatt gagccaaagg tcatcccacc atttcatagt cacgaggttc ccaaaccatt
     1681 ggtgagccaa ttcatgggcc acaacaaggg caacttgttg acggctagca aatgtagagt
     1741 tctcatcgac aaccaagtaa acttcacggt aggtcacaag accccagttt tccatagcac
     1801 cagctgagaa gtcaggaagg gcgatgtgga gagattgagg aattggatac ttaactccat
     1861 agtaatcttc gtaaaactcg atagagcgaa cagcgatatc cagtgagaaa tcaagatttg
     1921 aaagtggatg tgctttggtt gagtagacac ctaccagggt accattttta gttttagcgg
     1981 tcaccccttg caaatcacca gcaacaaagg ccaacaagta agaagacatg cgaggtgttg
     2041 tctcaaactt ccagatacct gtttccttac ggttttcaac atcgatttct ggcatgtttg
     2101 acaaggccaa ttcaccttct gcttggtcaa agcgaagaga gaggtcaaaa gttgctttgg
     2161 cttcaggctc atccacacat gggaaagctt cgcgcgcaaa atggctctcg aactgagtag
     2221 acaagacctc cttcttgact ccatcaactg tataataaga agggtaaatc cctgtcatgt
     2281 tgtctgtaat tttaccagaa aaggcaagaa ccaattcaac ttgaccagcc tcagccaatt
     2341 cgatatgaag ggcttcattg tcatggtcaa ctgtaaatgg acgagcttga cctgcaactt
     2401 ctacagaggt gatttccaag tctttttggt ggagggagat gcggtcactc tgtgcttgac
     2461 cagtgatggt cactttccca gaaaaagtct tggtctcacg actcaaatct aaaaataaat
     2521 cataatgttc aggaacaaat tgcttaataa aatgttcaac tgcttgcata gttttctcct
     2581 attctaagtt taagagctgc tgctcttctt caaacaaact tattatatca tattttgctt
     2641 gaaaaaaaag gattggacag cgatttccaa aactttcttc cttctaatag tctacagtgg
     2701 gtagaccttg cgaaattctt ctaaaacaac cttgctttca ggtgtaaaag tcagccctac
     2761 ttcacccaaa cagtctgtga aaaagtcctc atgaacctgg cgccaatagg caagtgattt
     2821 gtctccctca ccttccttaa aggcatgttg cgcagaaact tgattgaaga gttcaacaga
     2881 aacctttgta atttcgacaa tgcagacaga ctgattttga ctatctaaaa tgatatcgaa
     2941 ggtccctact tggggaagtg gttcgtcttc tagtacatag aggtcatagg ctgatgctgt
     3001 tgctgtcttt tctcctttaa acaccaaatc cgctaaaaga tctggttcaa ctccaaaagc
     3061 ccaagcatcg atttcatctc cgatcaaagg attgatttgc ttgtatttat tccacatttc
     3121 ttgcggtatc atgggtactc ctttgtaatt ttttactttc ttcttttatg tgtttaagat
     3181 gatctggatg gtcaatctct aaatcaaaaa tctctggaat agaactgtag tggataatgc
     3241 acttgatacc caactgattc attttttgta tgaaagaagt attcagatag cctgctacag
     3301 caaaatcaat cttgttcttt cttgctttat cctgcatatc tcttagcata tctaacatta
     3361 ttggactttc catatcatgc cattgactgt ttctcatagt cgcaaaaaca aaggaagtca
     3421 aatcattcat tccaactaca atctttgaaa tgcccgtttc cagtatacta gataagtcaa
     3481 aatacgctga cggtaattca atcatcgttc cgactttccc agtaaaaccc tgctgacgca
     3541 atactgtaat agcttgtttt aattggtcgg catcattgac aaaaggaaag ataacagata
     3601 gattggggtt ggtttgataa acttctgtaa cgacatgtgc ttcagcctga aattcatcca
     3661 aacacgccag taaacgcctg gttcctctat agccaaacaa gggatgccct tcgtcaaaaa
     3721 actctctagt ccccactaaa caattggctt ctgtattcgt taattcagaa aaacgatacc
     3781 aaacttcttc atctgagtac agggagcaga tagtctctag ataatctttt acaaattgct
     3841 gacaacttgc taatatgatg ttttgattaa gctctctcaa caaatattcc ccacgaatca
     3901 tgccgacgtg gtgaaatagt tgaggataaa ttttttcaag aattttttcg ccactaaggg
     3961 caagttgatt tctcatagcc attcacctcg tcttaaaaga tacaatatca tatataatta
     4021 tatcaaaatt tcctctagtt caaaagcttc ataaggttca gataactcat atcctaaaaa
     4081 ttctgctact tttttggaag cctcattatg agcatcccaa agtggaaaca tatctctatt
     4141 taaactctct agaatcattg cagcaccaag ctttttagca aatccatttc cttgttcgtt
     4201 tggtctagtt gcaacttcca cttcaactgc tttacggtaa actaaccctg aggaaatccc
     4261 agcaatcagt tcattatttt taagaatcac aaagccaaat ccacctttta aaacaaaatc
     4321 ctgataggac tcaaaatccc cctgtaaatc ctgtgaccat tcttccgtag aaaagcagtt
     4381 ataaatatga ttatcaatag gcacaatatt gtaaccttcc tctaaatgag taactagatt
     4441 atttaaaaat tcaacttgaa aatttgcctt atctttaaaa gaatagcgag taaaagaatt
     4501 tagccccact tttcgatgca gaaaatcttg ccaattagta ttttctgaaa taataatctt
     4561 ataatctaaa ccatattttc taacaaaatc ttcccaaaat ctggaatcca actcccctgc
     4621 taggtataga aaattcccaa catcataaaa gcttgttgtc ctatcattgt ttcggtagac
     4681 agttccaatt tgagatttaa gaccatagag aaccatattt ttcttccatg ttttaaatag
     4741 ttccattatc cctctacttc accttccaat tcatgtaaga agtcttgtcc agttctggca
     4801 atcctaataa ttcagactta accttcaaga ctaatggcga tgcattttct tctgtaatct
     4861 cttgaatatc catccaaata tatccaagtg aatcattcgc accatcagac acagcttccg
     4921 aaatcgtaac ttgaggtgca ctctcattca tttcaacatc atacaaggct atgacatggt
     4981 gaaccataaa attttttaac tcttccctga cgaaaacatc gtagattcga ggattagagt
     5041 agcttctaac agtaaatccc gtctcttcca taacttctct agtcagcgtt tccgtcagtc
     5101 cttcaccaag ttgctgactg cctccaggta gatcataccg atgttgataa gggcctctcg
     5161 ttttttcaat gcaaagtaac tttccatttt caaagcaaac acagtagacc ccaaagtgat
     5221 ttttgatttc catccaactc ctcctacttc aaagaccagc caccatctat tgtcagaatt
     5281 tgtccctgca tggcagatgc ctttccactg gccaaaaaga gactgacttc tgccacttcc
     5341 tctggttcaa tccagcgctt gattggggtt tcactagcca cccagtcagc taagccacct
     5401 gtctcaaaat ccgcagcggt catagctgtc ttgactgctc ctggagcgat accaaagacc
     5461 tgaatcctag cttcagcata gtctagagcc aactgcttgg tgaagccagc caaggcatgc
     5521 ttggatgaag tataggcgtg accacctcca cctgctaggc tagaagcaat ggaacacata
     5581 ttgatgatga ttcccttttt attttccagc atttgtgtca aataataccg agtcaactct
     5641 actggaataa tgtagttgat ttcaaaaatc tcttgaatgt cctgcgccgt ttgttccaac
     5701 agtggtttgt aagcatccaa aactccggca gtattacaca aaacatccac ctgagggcac
     5761 cagtcaaaaa taggttccaa gtccaaggtc aaatctctct gtaaaaagtg aaaatcaccc
     5821 tctaagagtg gcttttcacc ttggtcaact ccataaactt gatagccctt ctctaaaaag
     5881 aggcgagctt gagccaatcc gatccctgaa ctcactcctg taatgagtac acgtttagtc
     5941 atgcacttct acccaatccg ttgccaaaac atcacaaact gtcgggctcc acatggaaaa
     6001 accttctcct tctccagaaa cgttgattag gaaataaggt gtcatttcaa gtgcaagccc
     6061 attttgctcg atggtatcaa agagttggac atagttttcc gcacctcccc aaccagttcg
     6121 tacatatttt ctcttagcct ttaacccagg caggatctct tcaaatgtca tgtttttctc
     6181 ctttaattct acattcttca tttaattata gcaaaaaacc actttatacg gctttttgac
     6241 tgtgagttat tcaaacctgc tactacttac ggcaaattat tccctgcagc aagataaatt
     6301 tcataccatt cttttcttgt taagctaaag tttgccgctc ggctaacttc tctcaagtgc
     6361 ttaggatttg ttgtacctac gactgcctgc atttttgctg gataacgcaa tatccaagaa
     6421 atggcaatag ttgaagaggt tactccatat ttaatagcta aacgatcaag tacttgattt
     6481 aaagcttgaa atttctcatt tccaacaaag ttccctttaa aatacccgaa ttgcaagaca
     6541 gaccatgctt gaatgaccac atcgtgtaat tggcaatatt caaaaatgct gccatctcgc
     6601 atagctgctt gactatcttc catattaaca tgaaaagctg attcaaatcc tggagtaaaa
     6661 gccgcactca attgtagctg attaacagct aacggctgct tgacatcttt tttaagcaac
     6721 tccatcatca taggattttg attagaaact ccaaaatctc gaactttacc ttgtttataa
     6781 aggagatcaa aggcttctgc tacttggtca gattccatca aagcatctgg tcgatgaagg
     6841 agcaagctat ctagatgatc aatcttcaat ctttgcaaaa taccgtctac tgattttata
     6901 atatagtcct tagaaaaatc aaaataggta aattcttcaa tgcgaatgcc acatttggac
     6961 tgaatccaca tcttttctct taaatctgga cgatttttta ggacaagacc taacagttct
     7021 tcacaacgac cacgaccata aatatcagcc aagtcgaagg cattgattcc aacagaaagt
     7081 gctgtttcta caagctcttc aacttctttt acagacttat cttttattct catcattccg
     7141 agaacaattt ctgataattc tttgtcatct tgaccaagag ttatgtatct catcaaattt
     7201 ttctccttta atttctaaca ttcttccctt cattataaca aaaaaccgct ttgcaacggc
     7261 tttttgacta tacttcactc cattttatct tcttaaaccc acggaacaag acaaaaatgc
     7321 caataaagag gacagctaaa ggaataactt ttgcaaggaa aacatttgaa attcccatcc
     7381 actcataata acggagcaga gaacccacca caagatgggc aataatcata ctgacaaatg
     7441 gacgaaagac cgcttctttc caattccaaa taccgataac tagcgaaatc gtaaagacag
     7501 acaaactatc ccagggagcc ggaatataaa aggctccttc ttgtatgaag cttgccattc
     7561 ctacatatcc taaaacaact agaagaacta tagtcccaac aacaatgtaa gtgccaattt
     7621 tcattttagg agaatcttgg actaaacttc ttcgtaaaat tgtggccaca agtccaaatc
     7681 caatcagaaa aataagaagt tgccctaaaa atgtgagcaa attgactgtt aagagaggac
     7741 ctttagaaaa atcacttagt agttgataat aacgtaatac cgccaggaca agaattggcg
     7801 tcaaaaggga ctctttgata gaactgcgag gtgctccctt gagaatctct ttcattattt
     7861 ttttaggatt cttacctaga taatcctctg cactcatgcc atctcgttct gcttctgaga
     7921 aatctagcat catcaaatag atctgctctc tgagatagtc ttcatcatag agaaatccag
     7981 caagattaaa actttcccac aactcctcaa aatacttttg attctcctca gaaaactcat
     8041 gtagcaaagc gcttgtttct tcgtaatact tcattttctt catggtttaa cccccattct
     8101 taatcccttc tactttttga ctcaaatcgt cccattgttg ccaaaagact gagacacgct
     8161 cttctccttc tttcattaat gaaaaatact tccgatctgg accatctggc gacgggcgca
     8221 tgtcgcctct tatccattga tttttttcta acttttgcaa caaaggataa atagttcctg
     8281 gaacgatagt atcaaatcca gcctctcgca aagtctgaac caactcataa ccataccgct
     8341 ctttttgacc aatcatatcc aagacacaac cttcaagaac accttttaat agctgagttt
     8401 ctttcatcac ttttcccttc taatctattt tgtaatacct actagtaact tcacctatag
     8461 tatatcactt ctacactagt ttgtaaagca taatagttaa tactcttcga aaatctcttc
     8521 aaaccacgtc agcgtcgccc taccttatgt atggttactg acttcgtcag tttcatctac
     8581 aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
     8641 cattgagtat aaataaaaaa acagaactag cctgaactag tcctgtctac ttttacccaa
     8701 tcacacttcc atttggtaca gctggatcaa ctgtgagaag ggttaatttg ccatcatgtt
     8761 cagctgagag aatcataccc tggctgacat attttttcat cattttacgt ggtttgaggt
     8821 tagcaatgat ttgaactttc ttgccgacca attcttgttc atttggatag tattttgcaa
     8881 ttcctgaaag aatctgacga tcttctccat caccagcatc caagcggaat tgaagcaact
     8941 tatctgaacc ttctacttta gacacttctt tgacttctgc gacacggatt tcaaccttgt
     9001 caaagtcttc aaacttgatt tcatccttgt ttagtttgag ctcaacttcg tccggattcc
     9061 attctttttc gactgctggt ttattgcctt ccatttgttc cttgatatag gcgatttctt
     9121 cttccatatt tagacgtgga aagataggtg ttcctttggc aactacagtc acatctgctg
     9181 ggaagtcagc caaactcaag ttttcaagac tagaaacttc ttccaaacca agttgagtca
     9241 aaactgcacg actagtttcc atcataaatg gttcaatcaa gtgagcaact acacgaatgc
     9301 tggctgccaa gtggctcatg acacttgcca attggtcacg aagagcttca tccttggcca
     9361 agacccatgg tgcagtctca tcgatgtatt tattggtacg agagatcaga gtccagactg
     9421 cttcaagcgc acgtggatag tcaactgctt ccatgtgtgt atggaagtct gcgattgatt
     9481 gttctgcaac ctcagcaaga acatgatcaa attcagtcac accttctaca taggcaggga
     9541 tttgtccatc aaagtactta ttaatcatgg aaaccgtacg gttaaggagg ttcccaaggt
     9601 cattagccaa ttcatagttg atacggccga catagtcttc aggagtaaag gttccgtctg
     9661 aaccaactgg aaggttacgc atgaggtagt aacgaagtgg atctagtcca taacgctcta
     9721 ccaacatttc agggtaaacg acattccctt ttgacttaga catttttccg tctttcatga
     9781 caaaccaacc atgggcaatc aaacgatcag gtaatttaac atccaacatc ataagaagga
     9841 ttggccagta gatagagtgg aagcgaagga tatcttttcc taccatatgg aagactgttc
     9901 cattccagaa cttgtcaaag ttaccatgtt cgtcttgagc gtagccaaga gctgtcgcat
     9961 agttaagaag ggcatcaatc caaacgtaga caacgtgttt tggatttgat gggacaggca
    10021 ctccccatgt aaaggttgta cgagataccg ccaaatcttc caaacctggc tcgatgaagt
    10081 tgcgtagcat ttcattaagg cgaccatctg gcgtgataaa ttcaggatga gctttgaaaa
    10141 attcgaccaa acggtcttgg tatttgctaa ggcgaaggaa gtatgattct tcagaaaccc
    10201 attcaacctc atgacctgat ggagcaatac caccagtcac atttccagct tcatcacgga
    10261 aaacttctgc cagctggctt tctgtaaaga attcttcgtc tgatactgaa taccaaccag
    10321 agtattcacc caagtagata tcatcttgag caagtaagcg ttcaaagact tgtgcgacaa
    10381 ctttttcatg gtagtcatca gttgtacgga taaatttatc gtatgagata tctagtaatt
    10441 gccagagttc tttaactcca accgccattc catcaacata ggcttgaggt gtaataccag
    10501 cttcttccgc tttctgctgg attttctgac catgttcatc aagacctgtc agataaaata
    10561 catcgtagcc catcaggcgt ttgtaacgtg ctaggacatc acatgcgata gttgtgtagg
    10621 cagaaccgat atgaagtttc ccagatggat agtaaatcgg cgttgtaata taaaaatttt
    10681 tttcagacat aatttttcct ttccaggcaa atgaaacctg tttttctaac acttcattat
    10741 atcacatttt taatgaattt cgatagggaa atccatacaa aaacaagata gacgagtgtc
    10801 catcttgttg atctcattca taacgaaggg cttcaattgg atcaagtttc gatgccttgt
    10861 tggctggcaa gactccaaaa atcataccaa cactagccga aactgcaaga ctaaataggg
    10921 caactgggat tgatactcca acttctatac cttctattaa accttgcagt aacaaacctg
    10981 ctaaggcagt taaaccactt gcaattgtca agccaattaa gccacctaac aaggtcaaaa
    11041 tcatggattc aatcaaaaac tgaattaaaa tattggcacg tgttgcgccc aaagccttac
    11101 gaagaccaat ctcacgagtg cgctctgtca ccgaaaccag catgatgttc atgacaccag
    11161 ttcctccaac aaagagagaa atccctgcga tggaactaat aatcgtcgtc ataaaactaa
    11221 acgattgttg aatttctgca aatacaacgg actcatctgc cacctggtat tctccctgtt
    11281 gtaagcctgc aagctctgtc atttttcgtg ccagttctgg acccagagtt ggggttaaac
    11341 tggtatcatt cactcgaaag acaatactag ctatttcatc tatattaaaa ttcgcagcaa
    11401 gggagatatt ggtagtaata ggcaagccac caaacccata tatttttgac cttttagcct
    11461 ccggactagt ataaacccca atgacccggt aactaaatcc attgacttct acaaccttgt
    11521 taatagcctc ttgaggagat tcaaataaac taatggacaa ttcctcatct agcaaaataa
    11581 cacttgcaaa ctctttgaaa tcttgctctc tcagactacg acctgcaata atttcattct
    11641 taacagcgtc catgtaagtt ctgtttccac ctgtcaaatt agcattctca acctttttat
    11701 cttgataggt caagatggca ttcgttgaat tggttacata gtaactatcc actcccttca
    11761 gtttagctgc ctcttggacc caggattctt gcggttttgg cggttcaaca ggaatttcct
    11821 cttcctttcc agaaaccgta aaagatgatt gtttctgagt aaaagaacca tctttacttt
    11881 ttttaggaga gaaaaagacg ctaatatttt tctgagattt agtcatatct ttattgactt
    11941 gacgagatag ggaatcaccc aaagccataa tcacaacaac tgatgaaaca ccgataataa
    12001 tcccaatcat agtaagcaaa gaacgcatct tgtgagccat gatagatgaa aaggcaaatt
    12061 tcagattctg catcttagtt ttcctccttt cctaactgag cactgtcaga cgaaatgacc
    12121 ccatcccgaa tgacaatctg acgtttagca taggcagcaa tctcaggctc atgcgttacc
    12181 atgataatgg tttttccttc tttattcaaa tcaaccaata attgcataat ttggttacct
    12241 gttttggtat ccaaggctcc tgtcggttca tccgctagga taatagaagg attgtttacc
    12301 aaggcacgcg caatggctac acgttgcttt tgaccaccag ataattctga aggtaaatgg
    12361 tgactacgtt ctgtcaattc aaccttgtct aaatattcct cagccaactt gcgacgtttt
    12421 gaagacgaaa ctcctgcgta aatcaagggc aattctacat tttgcagagc attgagcttc
    12481 gatagaagaa agaactgctg aaagacaaaa ccgatttgtt ggttacggac cttagccagt
    12541 tgtttttcac caagcccagc cacttcttga ccttcaagat aatattctcc actggttggt
    12601 gtatccaaca tgccaatcgt attcatcaga gtggacttac cagacccaga tggtcccatg
    12661 atggctacaa attcaccctc attcacttct agattgatat ttttgagaac ctgcagttct
    12721 tggtcaccat tacggtagct tctgaagata ttttttagac taattagttg cttcatcagc
    12781 cttcacctct tttccttctt ccaaggaaga tgttggatta ctgatgacct tagcaccatt
    12841 cgttaaacca gaagtgattt cttgattttc tgcgtcagca tttcccaatg aaacctcaac
    12901 ttttttagcc ttttgttgtt catccacaat ccagacataa tttttactat catccattac
    12961 tagactgcta acaggaacaa gaatagcctt agttttgctt ttaacctcaa tgttgacaga
    13021 aaaaccctgt ttcaaatcac caacctcgcc tgtcacatca atagtataag ggtatttaga
    13081 acctgtatta ttcccggctg ctggactagc tgcttcacca ttgtttttag gatagtcaga
    13141 aatatagctt aatttcccag tccatttttt atcaggatac actttagaag taaagcttac
    13201 ttcttgacct acagaaaggt tggctagatt gtactcagac aattctccct tgacttgtaa
    13261 attttcattg ctgacaatat gaaccataac ttggctcgcc cctgttggag atttagaaac
    13321 attgctattg acttcgacta cagttccctc tagggtactg agaacagttg ttgcatccaa
    13381 ttgactttga gccttgctta attgcgctgc agcatctgca cgcgcatcac gggcatcacc
    13441 caattgagcg tcaatagaag caacagaatt tccagccact ggagttgggc tttgcaccgt
    13501 tgcatcttct cctcctactg gcgctggtaa ctgtggagcc ggagctgaag cggcttcatt
    13561 tcgtgcttga ttgagttcat tgatatgacg atctgcctta gctactgctc gactagctga
    13621 atcataggcc gcctgcgctt ctgaactact gtacttgact aaagcctgcc cttcgctgac
    13681 cttatcgccc acagaaacaa ggatttcatc taaatcaccc ttactagcat caaaataaac
    13741 atattgttca ttttttgctg ttactgtccc tgacaataaa acagaggagg ccacgcttcc
    13801 ttccttggca acaacaagat gagtaggctc atcttttaga gcagtctgag aaggttgtct
    13861 aaagagtaaa atccccccag cacccaatac aactacactc gcagcaccga ttgctgcata
    13921 cagttgccac tttttagctt taccattctt tttcttcata atgaaactcc ttttcttttt
    13981 tacaatactt tgctattata ccaaatttcc ctccagcaaa caatacagtt caggattaaa
    14041 caatcgttcg gaattttgct tttctattgt actagatata taatacacta tcttgactta
    14101 tttggcaagc ttttttgcaa ttttttgtgc gtagcagatt tttgcaatca aatcaaaaaa
    14161 agaataaaat ataactatca aaaaagacgc tctactattt aaaagattac aaaggagttt
    14221 tcaatgagag aatatgatat cattgctatc ggtggaggta gcggaggaat cgctaccatg
    14281 aaccgtgctg gtgaacatgg agccaaagca gccgttattg aggaaaagaa attaggtgga
    14341 acctgtgtca acgtcggttg tgttcctaaa aaaatcatgt ggtacggggc gcaaatcgct
    14401 gagactttcc atcaatttgg agaagactac ggctttaaga ctactgatct taactttgac
    14461 tttgcaaccc tacgtcgcaa tcgtgaagcc tacattgatc gcgctcgttc ttcttatgat
    14521 ggtagtttta aacgcaacgg tgtagacttg attgaaggtc atgctgaatt tgtagattct
    14581 catactgtaa gcgtaaatgg tgaactgatt cgtgctaaac atatcgtgat tgctacaggt
    14641 gcccatccaa gtattcctaa tattcctggt gctgagctag gtggctcttc tgatgatgta
    14701 tttgcctggg aagaacttcc agagtcaatt gccattctag gcgctggtta tatcgccgtt
    14761 gaattagctg gcgtactcca cacttttggt gtcaagacag atctctttgt tcgccgcgat
    14821 cgtcctttac gtggttttga ttcctacatc gttgaaggtt tggtcaagga aatggaaaga
    14881 acaaacttac cacttcacac tcacaaagtc cctgtcaagt tagaaaaaac tactgacggc
    14941 attaccattc atttcgaaga tggtactagt cacacagcta gccaagttat ctgggctaca
    15001 ggtcgccgtc caaacgttaa gggcttgcaa cttgaaaaag ctggagtgac tctgaacgaa
    15061 cgtggcttta tccaagtgga tgaataccaa aatactgttg ttgagggaat ctatgctcta
    15121 ggtgatgtaa cgggcgagaa agaactgact ccagttgcaa tcaaggccgg acgtacccta
    15181 tctgaacgtc tctttaacgg aaaaactact gcaaaaatgg attactcaac tattccaact
    15241 gttgtctttt cacaccctgc tatcggaact gttggtttga cagaagagca agctattaaa
    15301 gaatacggtc aagaccaaat caaggtttat aaatcaagct ttgcatctat gtactctgct
    15361 tgcactcgca accgtcaaga atcccgtttc aaactcataa cagctggttc agaagaaaaa
    15421 gttgtcggac ttcatggaat tggctacggc gttgatgaaa tgattcaagg atttgccgtt
    15481 gctatcaaaa tgggagcaac caaggctgac tttgatgcaa ctgtggcaat tcacccaact
    15541 gcatctgaag aatttgtaac catgcgttaa tcgaaacaaa agaagcaaag caaaaaacac
    15601 ttctaaatat caaaaagcga gtatttccgt agttggagat actcgttttc ttatgtttta
    15661 ttttatagta tgttgaagta agatatgcaa aaatcaatta
//
LOCUS       DAVLWA010000040        15108 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLWA010000040 DAVLWA010000000
VERSION     DAVLWA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.040"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(221..1828)
                     /locus_tag="VYN80_001218"
     CDS             complement(221..1828)
                     /locus_tag="VYN80_001218"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1541130.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2218..2805)
                     /locus_tag="VYN80_001219"
     CDS             complement(2218..2805)
                     /locus_tag="VYN80_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1541131.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2933..3382)
                     /locus_tag="VYN80_001220"
     CDS             complement(2933..3382)
                     /locus_tag="VYN80_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541132.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNYASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3387..3776)
                     /locus_tag="VYN80_001221"
     CDS             complement(3387..3776)
                     /locus_tag="VYN80_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1541133.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3804..4430)
                     /locus_tag="VYN80_001222"
     CDS             complement(3804..4430)
                     /locus_tag="VYN80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1541134.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKTPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4444..5007)
                     /locus_tag="VYN80_001223"
     CDS             complement(4444..5007)
                     /locus_tag="VYN80_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1541135.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5172..5406)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5460..5963)
                     /locus_tag="VYN80_001224"
     CDS             complement(5460..5963)
                     /locus_tag="VYN80_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1541136.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6156..6986)
                     /locus_tag="VYN80_001225"
     CDS             complement(6156..6986)
                     /locus_tag="VYN80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1541137.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6971..8653)
                     /locus_tag="VYN80_001226"
     CDS             complement(6971..8653)
                     /locus_tag="VYN80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541138.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8755..9012)
                     /locus_tag="VYN80_001227"
     CDS             complement(8755..9012)
                     /locus_tag="VYN80_001227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541139.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9019..9567)
                     /locus_tag="VYN80_001228"
     CDS             complement(9019..9567)
                     /locus_tag="VYN80_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1541140.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9582..10430)
                     /locus_tag="VYN80_001229"
     CDS             complement(9582..10430)
                     /locus_tag="VYN80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1541141.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10618..11967
                     /gene="trkA"
                     /locus_tag="VYN80_001230"
     CDS             10618..11967
                     /gene="trkA"
                     /locus_tag="VYN80_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1541142.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11971..13410
                     /locus_tag="VYN80_001231"
     CDS             11971..13410
                     /locus_tag="VYN80_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1541143.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13535..13792)
                     /locus_tag="VYN80_001232"
     CDS             complement(13535..13792)
                     /locus_tag="VYN80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1541144.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4524 a         3392 c         2741 g         4451 t
ORIGIN      
        1 taaaactttg ctagaaattg atttggcttt ccgaattaat ttgttcaaat tttatttcat
       61 ttcaatatac tgataaatcc attcactagc acagggcaag agggagtcgc tatttatttc
      121 aacggtagca ggtacataca gaggactcct atctcagata tactgcaata tcatcgtatg
      181 caaaaaacct ctgagatttt tctcaaaggt tctgattttg ctaattgctg ttctcaaccg
      241 ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt tggacggctt gacagttcag
      301 ggtgatactg acaagctaca aagaatttat tttcaggaat ttccacgatt tctaccaaac
      361 gattgtctgg agaaactcct gaaaagacaa aacctgctgc ctcaaactgc tcacggaagg
      421 cattattaaa ctcataacgg tgacggtgac ggcgttgcac cacttcttga ttgtgataag
      481 cagcagcagc cttagagcca cgtttcaact tagacggata aagtcccaaa cgaagggttc
      541 cacccatatc ctcaatatca atctgatcac gcatgatatc aatgataggg tattttgttt
      601 ctggtgcaag ctctgcagaa ttggcacctt caagacctaa aacgtgacga gcaaactcga
      661 tacatgtcaa ctgcattccc aagcagactc ccaacattgg aacatcattt tcacgcgcat
      721 agcggatggc ttggattttc ccttctgtac cacgttgacc aaaaccacct ggtacgatga
      781 tcccgtccgc atcagacaag agttctgcta cattctctgc tgtcacatca ttggcattga
      841 cccaattgat tttaacttct acatcattga catagccaga gtgtttcaag gcttcgacca
      901 ctgagatata ggcatcttgc aactccacat acttaccaac aagggaaatc ttaacttgtt
      961 tcttgaggtt catgaccttg tccaccatgg ctgaccattc tgtcatatcc gctgctggtg
     1021 cgtctaattt caaatgatca caaacaattt ggtccatccc ttgtgcctgc aagttcagtg
     1081 gaatttggta aaggtgttca acatccaacg attcgataac ggcttctggt gccacatcac
     1141 agaactgggc cagtttattt ttaattcctt gaccagctgg ctcttctgta cgaataacca
     1201 acatatttgg ttggattccc aagccacgca attctttgac agagtgttgg gttggtttgg
     1261 ttttcatttc accagcagcc ttgaggtaag gaagcaaggt tgtatggata tacatgacat
     1321 tatccgcacc cacatctgcc ttcatctgac gaagagcctc taggaatggc aaggactcga
     1381 tatctcctac tgttccacca acctctgtga taatgacatc agagtcggtc gttagagcgg
     1441 cacgcttgat tttttctttc aaagcatctg tgatatgagg aatgacttga acagttgccc
     1501 caaggtattc tccacggcgt tctttacgaa gaacttcact gtaaattttc ccagttgtca
     1561 cgttggaata tttgttgaga ttgatatcga tgaaacgttc atagtgaccc aagtccaaat
     1621 ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga ctcatggttc
     1681 ccggatcaat attgatataa gggtcaaact tttgaatggt tactttgaga ccacgatttt
     1741 tcaagagacg gcctagactc gctgccacaa tccctttccc aatggacgat accacaccac
     1801 cagttacaaa aatatatttc gtagacatag attcctcttt ctaaaatgct caaggtcttg
     1861 ttaactacga ggggatatag aaaacaagac cctactaata agaataatct gggacgactg
     1921 caccagattc acaactaata ctcttcgaaa atctcttcaa accgcgtcaa cgtcgccttg
     1981 ccgtagatat gtgttcctga ctttgtcagt cttatctaca acctcaaaac agtgttttga
     2041 gcagcctacg gctagtttcc tagcttgctc tttgattttc attgagtata aaatacaaga
     2101 agataacttc ttgaaaaaac aaaaatagct ccctaagaac tagggagccc cgacctctaa
     2161 aagaggtgcc caaacaatat gatacctaaa aataggataa ttgtcaatag ctaactctta
     2221 ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg tcgtcttcgt tgagatccac
     2281 atcttctccc aagtcatctg gagcgatttc gttgatttct gcatcataag cttccacttc
     2341 atttttttca tcatctggat tttcatcatc gtatgaaaga gctggatctg cttcgtatgc
     2401 atcttcgtct tctggatcat ctgcattgta gtcaatggca tctgaatcac catccataaa
     2461 ggcattgaca cgttttttct tagcttttgg tgctacttca tcgtcgtcat tttcttcaag
     2521 agcgatgatt tcttcgtcga tttcgtccac accataccat gaacgaagac cccatttgtt
     2581 gtccccaagt gagatgaagc taccgtcaaa gttcaactct gtgtagaaca aaggcaaagc
     2641 ttcgcggata tcgctgtttg atgttccaag gtagttttga atttcgttta caagatcgct
     2701 aaaatgcatc tcatgatcgc gaccacgaag ttccaagata gcacgcgcta cctcaatcat
     2761 agatagttca cttttttctt gcccagcaaa tacttctaat tccaaagcgt ttctcctcat
     2821 ttatactact atcgccagag cgaacagact ctgacctcat tttatcattt actctttatt
     2881 ttacgataat tttgcggaat agtcaaaggt taagggggag aaagtggcag gattagacta
     2941 attccaatat aaaactcctt cctttttctg ttgctccatt ttccacaaat ccaagcgact
     3001 tgaaacacct cctagaagca taattgtagg tgtagatttc cttgactctc aattctttcc
     3061 atccttttac tcgagccaat tcaatcaaag cacttagaat cttttttcca agtcctcgat
     3121 gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt gtaatatccc
     3181 caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca ccatgccgat
     3241 ccaaatagga atacatggct tccaaggtcg cttgactgta aggaagcttc accccatcta
     3301 cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa tgattgacct
     3361 tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc tgcttgatta
     3421 aaaccatcac accagttata ccattttgct tcatacccat cttgagctag gatacgatct
     3481 tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata gagatgatat
     3541 agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc tgccaataaa
     3601 gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa ggcaagaata
     3661 ctgattggat cgacttggat tgtttctgcc gactggtaaa gcatctgaat atcttgcaac
     3721 ttcttttctg ataaatatag ttgagtcgga tgaagatctg ctatattgac tttcatttct
     3781 ttctcctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca ttggaagcgg
     3841 agcttgtcat agaagggaaa ctcgataaac aggactccca agcccacaca gagactggca
     3901 aggacgtctg atgggtaatg aactcccaga tagactcttg ataccagcac actgactagg
     3961 tagaggccaa ggacgatttg tacgattttt ctccagactg gagttttaat tcgttgactg
     4021 agaatgacaa tcagagtacc taccatcaaa gttacagcca gagaatgccc acttgggaag
     4081 gaaaatccct tctcttccac cagatgtaag atagctggtc gtgggcgctg gtagatattt
     4141 ttaaaggtca cgattaaaag acctgccaaa gccagatttc ccagcatgaa gaaactttct
     4201 atcttccatc gcttacgata aaagacaaaa gctgtaatga caacccaagt gataatcact
     4261 gggatatcaa tcagacgtgt gagggatcga aaaagaatag tcaaataatc tggtaagtct
     4321 cctcgaatgg cagtctgaat cgattggtca aaattgacca acatttcagg gtaaaatttg
     4381 accatatagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat taaaaatgtt
     4441 tgtttatctt tcatagtgtt ttaaggttgg tttcaaaagg acgtacaaca accagaatga
     4501 aacggaaaag attacaccct caatcaagtt aaaaggtaat accatggtca ttaggtagtt
     4561 ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca aaggaacagc
     4621 ataaacatag ttgagaacca acatggccaa ggttaaacca acagttccag ctagagagcc
     4681 tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata cgatgacaaa
     4741 aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc cttggctgtt
     4801 aagaagcaat ttcaagagtg agcgaagcaa gagcactcct agagcagcag gcaaatccat
     4861 gaccaccaga cccacaagga ctggcaagat actaaattcg atcttgagga aagatgccgc
     4921 tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata aaattgcaat
     4981 ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc aattttctat aaaatcagaa
     5041 gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag ttccctctta
     5101 ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta tcaaaacaga
     5161 caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg aatctcacca
     5221 catcagcttg cgctcgcgga cttctttaaa gggaaagaaa tagagatttt tcttttagac
     5281 taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca aagtataaaa
     5341 ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc tgccctgaag
     5401 actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat tttcattttt
     5461 tatttcaatt tatccacttc ataggtatgg acgagctcaa gacctgcaaa gttctgctgg
     5521 cgaagagcct catagacaat catgcagacg gtattagaca cattgagact gcggacatgt
     5581 tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa atcttcaggt
     5641 aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga taaatccacc
     5701 tcagaataga ttttttccgc gaatttcgaa atcagataga gttttccctt catctgagat
     5761 atgaaatctt ctaaactctc gtaaaaataa atctctagct tatcccagta atccaatcca
     5821 gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat gtggagggga
     5881 gaattggtcg cagcgcaagt acgcgcgata ttgcctgtat tttgtggaat ttgaggttca
     5941 aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa aaatagccac
     6001 actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact tacctcacaa
     6061 caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag aatcattgtc
     6121 aatagaaatg acttgatttt ttcaattttt tcaagctatt tccaagggtt gtaaaatcgt
     6181 ccctgattct gcaagataag tagtaaacta actactaaaa acaaggttgc caagagcaag
     6241 gtaatatagt ctcctttttt caaggcctga taactatacc atgtgcgttt tttctctttc
     6301 ccaaagcggc gaagctccat ggcagtcgca atggtatcaa tgcgttctag cgagctaaaa
     6361 atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga agctttcttg
     6421 gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc ttcctgcaaa
     6481 tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg cacaccaatt
     6541 tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat aatagccaga
     6601 ggaatggtgc aaagatactt aatggccaaa tttagcagat aaaagagctc ctggctggtt
     6661 agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag tccaacccca
     6721 tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatac cgtcgcaaaa
     6781 acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag aaagactgaa
     6841 aagatggcaa tcagcaagag cattctggta tcatagctaa tcatggccgc caatgatacc
     6901 agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt atctctatgc
     6961 tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat gccgttaaat
     7021 ccagtggatc cacatctagt ttcttagcca agttaaagat ggaggtttct tttagattgg
     7081 cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg gcaatcaatt
     7141 ctccatccac catgacaagg acccggtctg aataatccag catcaattgc atatcatggg
     7201 taatcatgac aatggtatgc cctttttgat gtaactcttc gagaaattcc ataatctcag
     7261 tatagttctt ctgatcttga cctgcagtcg gttcatctag gagaataatt tcagctccta
     7321 agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc aaatgacagg gcagaaatag
     7381 gccaattacg gaattcataa agtccacaga ttttcaaggt ttcatagact ctcgtttcaa
     7441 tttccttctc atccacacct cgcaaacgga gccctagagc cacctcatca aaaatcatat
     7501 tggttgaaat catttgatta ggattttgta gcacatagcc tactcgttcc gcccgctctg
     7561 caacagaatc gccttttata tcctgttttt cccaaagata gcgtccttcc gtctgaataa
     7621 agctacttat agccttggct agagttgatt tccctgctcc attttttccg acaatagcaa
     7681 tcttttcacc ctttttaata tctaaatgta gggattttaa aatcggtcta tcatcataag
     7741 agaaagacac gtcctctaat ttaaagagtg actgcaattc tggagtttct tttgccagtt
     7801 cattctgcag ctgaacctga ccttttgaga tagacatgtt atccagattc gctaactgtt
     7861 cttccttgac taagtccaca cctaattgac ggagagtcgt tagataaagg ggttctcgaa
     7921 ttccattttg agtcaataaa tcagtcgcaa gcaactggtc agggctccca ttaaaaagga
     7981 tacgaccatc gcttatcaag acaatccgat ccacagggcg atgcagaaca tcctccaaac
     8041 ggtgctcgat aatcagagtc gtcgtcccct cttccttatg aatctggtca atcaattcga
     8101 taatatcctg acctgacttg ggatctagat tggcgagtgg ctcatcaaac aagagaatcg
     8161 gactttcatc aatcaagaca ccagccagac tgactcgctg cttttgtcca cctgacaaat
     8221 cctgaggacg ctgatccagt aaaggaagaa ggtccagctt ttcagcccat ttataaacac
     8281 gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc aaatcttctg
     8341 ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta accagatgag
     8401 acttatcata gatgctcata tcaaaggcta cttgaccctt tatcaaaaat tctccatatg
     8461 tctgaccctt gtaaatattg ggaataatcc cattcaaaca ctgacccaag gtagatttac
     8521 ctgacccaga tggtccaaca attaagactt tctctccctt gtaaatggtc aagtctatcc
     8581 cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc cactcaatta
     8641 tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta tcataaatca
     8701 agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc tatcctagct
     8761 tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct tccccatcat
     8821 tttatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt aggtatggtt
     8881 actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga cttcgtcagt
     8941 tctatccaca atctcaaaac actgttttga gcaacctgcg gctagcttcc tagtttgctc
     9001 tttgattttc attgagtatt agtccttttt caaacttcct gcacgagttt gggttcctgc
     9061 ataggcaagt aagagaagag ttcctgcaat agctacagat acaccattgg caattcccgc
     9121 aacaatccct tgtgcaaata ctttttctgc cgcttcttga taaatcacaa catctccaag
     9181 tggtgccaag acaccccaaa caagggcatt tgcaagtagt tgaatgaggt taaaaataag
     9241 aatatctttc cagtcaaaaa caccattgat cactcgaacg tactttctaa aaagtcccac
     9301 aactaaacca aagagtccgc tagcgataat ccaagtccac catagaccat aaccagcaag
     9361 agagtccttg attgcatgac caatcaaccc gacaagcaaa ccgataatcg gtccaaaaat
     9421 aatagaaagt agcgcttgta ccgcatactg aagctggatg cttgtatttg gaacaggggt
     9481 tggaatgttg atcatcccga tgacgacaaa gagggcagcg ccaattccga cagcaacaac
     9541 ttgtttaatt gtaaatttga tttccatact attctcctat tttatccttc tattttcttt
     9601 atttcaatgg tccaagatga accgacacct acattatagg ccttggcaaa ggaaccttgg
     9661 ttgatagcca aacctaaacg atagagagag ttgatgtaaa ggatgggttg cccaattctc
     9721 acatctgcaa atgatttgcc atagacaacc tgattttgat agaccagcat atcagcatga
     9781 tagatggtca cttcaaaacg atcaccaaat tctggttcca gcttgtaaaa ttcttcccgt
     9841 gtgatagagg tccaaagcga accgaaacgc acatccagaa tatcaatggc tcccttcacc
     9901 agatgatctt ctatgatggt cgctacgact ggaagctcta caatctggtc cacactgagc
     9961 tctggcccta cttcctcaaa agtaatgtga ccactggcca gtttagcacc agtataggca
    10021 tagacatcac gaccgtggaa ggtataagaa tgctctgtgt tttgacgcct attggccacc
    10081 tcagaaatct cacgaatggc tacaatgcca acgtgtttct tgataaagga aagcgtccca
    10141 ttatctggcg tgacaatgta ttgatttttt gcagtcttgg caactacact cttacgtttc
    10201 gaaccgacac ctggatcgac aaccgataca aacgtcgttc cctcaggcca gtaatccacc
    10261 gtctgaaaga gacgatagct cccctcaaaa atattataag gcgtgatatc gtgcgtcaag
    10321 tgatggattt ttaaggttgg agactcttct aaagccactc caatcatagc cgataccgca
    10381 ccatcaacca gaccaaagtc tgattgtaat accagtaaat tattattcat tttatctcct
    10441 tgtatatcct ttatcatacc atatttcaga gaaagtcgct aatagctatt tcaattggtt
    10501 agggtatagt tttaccttat agcgaatttt cactaaaagc tcttgttatt agctagtagg
    10561 tgcatttttt cttgaaaaag ggtatgatag ttacatcatg aaaaagtagg ttttattatg
    10621 aaaattatcc ttgtcggagg gggaaaagtt ggttttgccc tctgtcgctc cttggttgca
    10681 gaaaagcatg atgttttgct gattgagcaa gacgaagctg ttctcaatca tattgtcagt
    10741 cgctttgata tcattggtat ccttggtaac ggggccgatt ttgccattct tgagcaagcc
    10801 agcgtccaag attgtgatat ctttatcgcc ctgactgagc acgatgaagt caacatgatt
    10861 gcagcagttc tagccaagaa aatgggagct aaagaaacta tcgttcgggt gaggaaccct
    10921 gaatattcta actcttattt caaggaaaag aatattctcg gtttttctct tatcgttaat
    10981 cctgagctct tggctgcccg cgctatcgcg aatatcattg acttccccaa cgccctgtct
    11041 gtcgaacgct ttgctggtgg acgcgttagc ctcatggaat ttgtcgtcaa gtccaccagc
    11101 ggtctttgcc aaatgcccat ttctgatttt cgtaaaaaat ttggtaatgt cattgtctgt
    11161 gcgatagaga gggatcatca aattatcatt ccaagcggtg acatgactgt acaggataaa
    11221 gatagaatct ttgtcactgg taaccgtgtc gatatgatac tcttccataa ttattttaaa
    11281 tcacgcgccg tgaagagcct tctcatcgtt ggagcaggta gaattgccta ttatctactt
    11341 ggtattctca aagatagtcg tatcgataca aaagtcattg aaatcaatcc tgaaatcgcc
    11401 agcttcttta gcgagaaatt cccaaatctc tacatcgttc aaggagatgg aaccgcaaaa
    11461 gatatcctgc tggaagaaag tgctcaacac tatgatgccg ttgcgactct aacaggagtc
    11521 gatgaggaaa atctgattac atctatgttc cttgacaggg taggtgtaca gaaaaatatt
    11581 actaaggtca atcgtaccag tctcctcgag attatcaatg cgcctgattt ttcaagtatc
    11641 atcacaccta aaagcatcgc tgtagatacg attatgcact ttattcgtgg tcgagttaat
    11701 gcccagtatt cagaccttca agccatgcac catctagcca atggccaaat cgaaaccctg
    11761 caattccata tcaaggaagc caataaaatg actgccaaac ctctttctca actgaaattg
    11821 aaaaaagggg ttcttattgc agccattatt cgaaagggca agactatttt cccaactggg
    11881 gaggatatgt tggaagttgg agacaagctc ctagtaacaa ccttgttgcc aaacatcacc
    11941 aagatttatg acttgattgc gaggtaagaa atgaataaaa gtatgattcg ttacctcctt
    12001 tcaaagttac ttttgattga agctgttctc ctcttggttc ctgtggctat cgctgtctat
    12061 taccgtgaat cgagccaagt ctttacagcc ctcttttcga ccatagggat tctcgtattg
    12121 ttaggcggtt cagggagttt acagaagcca aaaaatcaac ggatttatgc caaggaggga
    12181 gtcttgatcg ttgccctctg ttggatcctt tggtctttct ttggtggtct cccctttgtc
    12241 ttttctgggc aaatccctag ccttattgat gccttttttg aaattagttc tgggtttaca
    12301 actactggag caagtatttt gaacgacatt tcggttctca gccgttccct cctcttctgg
    12361 cgaagtttta cccacttgat tggagggatg ggagtgcttg tttttgcact tgctattatg
    12421 gacaatgcca aaaatagtca cctagaagta atgaaggctg aggttcctgg tcctgtcttt
    12481 ggtaaggttg tatccaaatt aaaaaacact gcccagatcc tctatctcct ttacatagcc
    12541 ctcttttccc tctttgtcat catctattat ctagccggta tgcctctcta cgatagtttt
    12601 gtcattgcta tggggacagc gggaactgga ggatttaccg tctataacga tggaattgcc
    12661 cactatggta gctcactgat tacctatctg gttagtatag gagttttggt ttttggggta
    12721 aatttcaatc tctactacta ccttatgctc cgtcgggtta aggccttctt tggagatgaa
    12781 gagcttcggg cttacttggt catcgtgctg gtttctacag gcttgattag cctcaatact
    12841 ctctacctct acccagggtt ttcaaagagc tttgaaatga ccttcttcca ggtttccaac
    12901 atcattacaa caactggttt tggatatgga gatattacca actggcccct cttctcccag
    12961 tttatcctcc ttttcctcat ggcaatcggt ggctctgctg gatcaactgc aggtggactc
    13021 aagattattc gaggcctcat cctttcaaaa attgccaaaa accaaatttt gtcaatccta
    13081 tcgccccacc gtgttttgac cctccatgtt aataaaatgg tgattgacaa agatacccag
    13141 cataagattc tcaagtactt tgtcatctat gctatgattt tgctagcgct tatctttatt
    13201 gtcagcctag atagcaatga ttttctggtc gtgaccagcg ctgtctttag ctgtttcaat
    13261 aatatcgggc ctattctagg aaccacttct agtttctcaa tctttagtcc catctcaaaa
    13321 attctcctct cctttgcaat gattgcaggg cgcttggaga tttacccaat cctccttctc
    13381 tttatgaaaa gaacttggtc taagagataa aatacaaaat ccctcttttt acaattgtga
    13441 aagagggatt tttatattaa aaagggggaa cgtttttaaa tccaagcctg tctgtaagca
    13501 aagttcttac tcaaataaaa gatggaaaaa cttactacac agcttggtaa tgctccaata
    13561 caaattgtaa ggctttactt ggattctttg ttaaatgaat atattccatt cctctggtag
    13621 caactatctg aatacctaat ctttctgcat ccaccttcat ctctctataa taactccgaa
    13681 cttgtggagc cagaataatt aaatcataaa cacccataat atcataatga gctccgtacg
    13741 ctcctgaatt cgcaatcact ctaacttctg ctaattgagc gccctcgtta attgcattgg
    13801 ctaattacgc acttgttcca gaccctgcac aaagaaccag tactttcaac tccttacttt
    13861 ttattacatc tatctcacca ggcattattt cacttgtatc ctcctctaaa ataatatcat
    13921 ttgagcttgc aatatctata acctgtagta gatatctcgt atttagacaa tatgaaaaca
    13981 agacgacttc catataggaa accgccttct cgctatgttg agtgatttat attaaaataa
    14041 cttttcttct agctgcattt tattattata aaaacattca tcataacccc cagaacttaa
    14101 ataacaattt ttattcaaga tacatactcc tagaataaac tttatatgaa attctcattt
    14161 ttgtttttac aattctcctt agttaaatct tgtttaatat atgttttaca tatagtattt
    14221 agcgccacat agtactgaac tctctccaaa aacggttatt cctctttgaa tagggcgtta
    14281 tcacaagaaa agcatctcca cgtttcaact tcatatggct caaaaacaat caattgatgc
    14341 taaaacctgt acctagatgt ttcggttcat aaaaccatga aactgtaaaa gtggatgaaa
    14401 ttgatagcga tagtcaaatc aagaggcatc ataactctaa aaagtcacaa tatataagtt
    14461 catcctcgga aaaatatcat tctaattgtt gaaatgccta catgaaaaga aacgtcaaat
    14521 gctcatgaaa caacgaatac aggtatcaaa actatgacaa aacaaatccc taaatttact
    14581 aaagacactg ctcaacttta cacctgtaaa tggttgttgt ataataaagt tacaaagatg
    14641 tacgaccaca ctgttgtaaa tcatagtgtt cgcgaatata ttactgatag catttctaca
    14701 aatacaagta aagagagcgg atgagattca aacgaaatat gtcagtgctt tggcattcct
    14761 agccttcata tcatttaaag aattctatag acaaaatttt ttccaataca gacactcgta
    14821 acaactgctt catttttcta ccaacatatt taggaacagg ataagataca agagtattaa
    14881 tccatagctc agttctatac caatctaaga caaataagct aaaaaaacga ttgataataa
    14941 gcaaatagat tccaaatttt ctctatctgc tcattttaat aaacaatact agtgtaacta
    15001 tccttccagt cagaagcttg tcaaatcaca ccgaaaattc ttctaaaatt tatctcgtta
    15061 ggcaatcaag caaaaactcg acgatagtac aaacattatc atacagga
//
LOCUS       DAVLWA010000041        14625 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLWA010000041 DAVLWA010000000
VERSION     DAVLWA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.041"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            86..1147
                     /gene="dinB"
                     /locus_tag="VYN80_001233"
     CDS             86..1147
                     /gene="dinB"
                     /locus_tag="VYN80_001233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1541145.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSN
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1294..2139)
                     /locus_tag="VYN80_001234"
     CDS             complement(1294..2139)
                     /locus_tag="VYN80_001234"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1541146.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2395..3254
                     /locus_tag="VYN80_001235"
                     /pseudo
     CDS             2395..3254
                     /locus_tag="VYN80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3555..5444)
                     /locus_tag="VYN80_001236"
     CDS             complement(3555..5444)
                     /locus_tag="VYN80_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1541147.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEEVLNQMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            5582..7147
                     /locus_tag="VYN80_001237"
     CDS             5582..7147
                     /locus_tag="VYN80_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1541148.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            7147..7887
                     /locus_tag="VYN80_001238"
     CDS             7147..7887
                     /locus_tag="VYN80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1541149.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(7996..8211)
                     /locus_tag="VYN80_001239"
     CDS             complement(7996..8211)
                     /locus_tag="VYN80_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541150.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(9082..10332)
                     /gene="ilvA"
                     /locus_tag="VYN80_001240"
     CDS             complement(9082..10332)
                     /gene="ilvA"
                     /locus_tag="VYN80_001240"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1541151.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(10382..10738)
                     /locus_tag="VYN80_001241"
     CDS             complement(10382..10738)
                     /locus_tag="VYN80_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1541152.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(10976..11212)
                     /locus_tag="VYN80_001242"
     CDS             complement(10976..11212)
                     /locus_tag="VYN80_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541153.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(11319..12341)
                     /gene="ilvC"
                     /locus_tag="VYN80_001243"
     CDS             complement(11319..12341)
                     /gene="ilvC"
                     /locus_tag="VYN80_001243"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1541154.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(12407..12883)
                     /gene="ilvN"
                     /locus_tag="VYN80_001244"
     CDS             complement(12407..12883)
                     /gene="ilvN"
                     /locus_tag="VYN80_001244"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1541155.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(12876..14576)
                     /locus_tag="VYN80_001245"
     CDS             complement(12876..14576)
                     /locus_tag="VYN80_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1541156.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         4490 a         3257 c         2604 g         4274 t
ORIGIN      
        1 gattgctcaa aacactgttt tgaggttgca gatagagctg acgtggtttg aagagatttt
       61 tgaaaagtat aaaaaggtgc taggcatgtt gatttttcct ttgttaaatg atttgtcaag
      121 aaaaatcatc catattgaca tggatgcctt ttttgctgca gtggaaatca gggataatcc
      181 taaactcaga ggaaaacctg tcattattgg aagcaaccct cggcaaacag gtggacgggg
      241 agtcgtttct acctgtagtt atgaggcaag agcttttggt gtccattctg ccatgagttc
      301 caaggaagct tatgaacgtt gtccccaggc tgtctttatc tcagggaatt atgagaaata
      361 caagtctgtg ggactccaga ttcgagctat ttttaagcgc tatacagatt tgattgaacc
      421 catgagtatt gacgaagcct atttggatgt gacagaaaat aaactcggta tcaagtcagc
      481 ggtcaaaatt gctcgcctca ttcaaaaaga tatctggcaa gaactccatc taactgcttc
      541 tgccggtatt tcctataaca agttcttagc taaaatggcg agtgattatc aaaaacctca
      601 tggtttgaca gtgattctac ctgaacaggc tgaggatttt ctcaaacaaa tggatatttc
      661 caaatttcat ggagtaggaa aaaagacagt agaacgtctt catcaaatgg gcgtttttac
      721 tggtgctgat ttacttgaag ttcctgaggt gaccctaata gaccgttttg gtagactagg
      781 ctatgatctg tatcgaaagg ctcgtggcat tcacaactct ccagtcaaat ccaatcgcat
      841 ccgtaaatca atcggcaagg agaaaaccta cgggaagatt ctccgtgctg aggaagatat
      901 caaaaaagag ctgactcttc tatcagaaaa agtcgctctc aatctacatc aacaagaaaa
      961 agctggaaaa attgtcattt tgaaaatccg ctacgaggac ttttcaactc ttaccaaacg
     1021 aaaaagtctt gctcaaaaaa cacaagatgc tagtcagata agccaaatag ccctgcaact
     1081 ctatgaagaa ttaagtgaga aagaaagagg tgtccgccta ttggggatta ccatgactgg
     1141 attttaaagc tcaatgaaaa tcaaagagca aactaggagg ctagccgcag gctgctcaaa
     1201 acactgtttt gaggttgcag ataaagctga cgcggtttga agagattttc taagagtata
     1261 aaaccttgaa gagttgcccc ttcaaggttt ttcttataca aataaacgga caaaactgta
     1321 aagtagcaaa acactaccaa gcacgatacg gtatttacca aaaagggtga agtcgtgttt
     1381 tttcacatag ctggtcaaga agcgaatagc caccatgctg accgcaaaag ctactcccat
     1441 cgcgaccaag agcaaaaaca attgcccaaa gctcaagagt tctccggctt tcacaaattt
     1501 gaaaatcttt aaggcactag ctccaaacat aacaggaatc ccaagataga aggtaaattc
     1561 tgtcacaact gaacgactgg ttccatttaa caaaccaccg acaatcgttg cacctgaacg
     1621 gctagtccct ggtaaaagag caagaacttg gaagagtccg atatagaaag cggtcgtata
     1681 aggaagcttg tccaactctg ttacacttgg ctcgatagca cgcgctttat tgcgcttttc
     1741 caaatagatg aaggcaatcc cgtagataat caacatgaga gcaactgaaa ccatgttatg
     1801 gaagtgggta tcaaaccaat catcaaattt aaagacacca agtaaaggta aagtagcaat
     1861 caagaccttc aaccatagtc tccaagtctt acgaacttcc tgtttgtcct tagtcggttt
     1921 aaaaggattg agcttgttaa aataaatcac cataactgct aaaatagcac caagctgaat
     1981 cacgacatta aacatggaca taaaggcttc attttgattt tggtattgga taaactcctc
     2041 tgctaaaatc aagtgacctg tactggaaat cggcaaccat tccgtaattc cttcaacaat
     2101 cccgaagaag atagatttta aaatttcaat aagatacata tatttctcct tatattatat
     2161 ttaacgcagt tagctactgc caaagcccaa gtggtatact tggaataagc cactgtggat
     2221 tagttcattt cctccaatcg cttgacgatt tccgaaaggg tctaaccaca gctttttgtt
     2281 tcaattggta atgagtttga taacgcatag acgttttatc tcgtctaagg taacaaggca
     2341 aaaagttcgt ggataaccaa gtagacttga aatactatat ctggaggtac tcccatgctt
     2401 tatgtgggca ttgatgtcgc taaaaataaa cacgatgtta cagccttgaa tgttcgagga
     2461 aaactgttct taaaccactc actttttcaa ataataaagc tggttttgaa ctcttagatc
     2521 tgtcttttcg acagctcaac caagactgtc tcatcgctct taaacttctt tctgacccca
     2581 atcgtgaaca atttcaacac gataatcggc aagtagaact aaaaatactg gctagacata
     2641 ttcatcgtct caagaaaaaa cagtctgatt ggaaagtaca atacactcgt tgtcttgata
     2701 tcatctttcc tgagttggat aaaatcgttg gaaagcattc agaatatacc taccaactct
     2761 tgacacgcta ccctaatcct cagaaaaggc ttgaggcagg atttgataag ctgatagaaa
     2821 ttaagcgatt gaccgcttct aaaattcagg atatcctctc agtcgcacct cgttctatcg
     2881 gaacaacatc tcctgctcgt gaattcgaaa tcatcgaaca ttacaagagg ctcattgaca
     2941 aggcggaaac atgtgtcaat gacttgatgg ctgagttcaa ctcggtcatc acgacggtta
     3001 ctgggattgg gggtcgttta ggggcggtca ttttagccga gattcgaaat attcatgcct
     3061 ttgataatcc tgctcaatta caagctttcg ctggactgga ttcttctatt tatcagtcag
     3121 gtcagattga tttagctgga agaatgatca aacggggttc ccctcatctg cggtgggcac
     3181 tcatacaagc tgccaaagca tgcgctcgct tttcacctgc ttttaaggcc tatcttaaga
     3241 ctaagttaga ataaggaaaa cattacaatg tagccatcat ccaccttgca aaaaaactta
     3301 tccgaaccct gttttatatc ctaaaaaaga gctgccattt gacgaacaaa aagtgatgta
     3361 atttctgaaa ttaacacagg attacctttg aaaaaatgga ctttttcaag ggctttttgc
     3421 cgtgctctaa aatcatcgcc aaatcatcaa attgaaataa gaagtagttt agataaaact
     3481 attgactttt cttatagttt gtctttcttc tatcttccat tatatcatac tttttcagtc
     3541 tatggtagct ctctttaaaa ggctccgata ctgccgccac ctccgcctcc agagaagcca
     3601 ccgccagaac ttccacttcc agaagatacg gagtaggtac ttgctgtatt tgcgacacta
     3661 gcataatggc tcatttgcgc gcttgaatga taaaacatac tgtgccagcc ataagctaca
     3721 tagagattga tatctggatt ttcaacttga atctgatgaa ccttcatcaa atgactaacc
     3781 ttgtccgcat agccaaataa ggttgcatag accaagagac gattccaaac aacaatactt
     3841 tccaattcag ccttatccag tcgtgcaatc tcacgtaaca tattttcaaa actggtccag
     3901 agatagtaga cctcagctcc cgcttcattt agaacaccat cacgattatc tagtcgaagc
     3961 ttccaataat agaaaacaga caaaactaac cctagaaaac caagtattgg caaagggagg
     4021 taaagatagc catgaacgtc taaactgtac aagaacaaac caaatccgat aaataggggc
     4081 aagatagtca agacacccat acccacttgc aaagcctttt ccaaaccagt taaaggacga
     4141 tagtaatctg ggagtcccca gaagacaact cgctttctca ctccttcttg catctggttc
     4201 aatacctctt caaaagaaga tttgagctga atccctcttg cttgaatccg tttctcatca
     4261 gcagctttag ctccgcgata aaggctgtca gataccttgt aatccgcaaa caaattggaa
     4321 agagtggctt cttttttgcc tgaaaaggct agatttagac agtctctctc aaatcttgac
     4381 aaaccatctt ctttcaccaa tctcaagcca actgcatctc cttctgaaat aatagaaaca
     4441 ttcccacggt ccatcacatc tagcaaggta gcttgaataa gttggtcaaa agtgaatttg
     4501 ccagccccct tgactaaggg actcacttcc tccaaggagg tcgagtagac agcctctgat
     4561 aaaaccatag gctctaattc cattggtggt tcatagagac gatgattttt ggcatatttg
     4621 actgaaggag tggtctttct tctataaata aaatagaagc agacactcaa taacaaggag
     4681 atagaaagga tcgaagggag gacccaagta acaagttgtt tactctgatc tttttctcta
     4741 acaatcgagt cttctatctt attaaactct tctaaacgat tccctttcaa tccctgatcc
     4801 ctagcgctag caaaatcggt ccgaggccag taggcatgca actcaactcc acgcttagcc
     4861 ggaagattgt ctaaacggat agtataatca aggttactct tttcaatcgt tccctctcta
     4921 aaaagtttcc ctgtatggaa aaagagtttt tcagccccct tgtctcccct tacatgaaat
     4981 tcaaactttt caatagactc tgaactatct gtcagaggtt gccaatttaa ttcagcaata
     5041 tcatcataaa ggaaaagtaa attttttaag ttccagacga ggtcaacttc aactatgtcg
     5101 ccctcctgac ctggattata gactctcaca gtataaccat ccgcttcttc tgttacttcg
     5161 ctagtcacat ctgctagttc tgcaccgttt ttcgcggcct gaacctttgg atgagggtca
     5221 atgtcaaacc cgctaggcat cttaccagca cgtccaagtc ccacgatttg tcccttaaag
     5281 tcctcctcaa actggtaaac tatcttctgt ctaaactctg ccgtattatc tgcatgaata
     5341 tacaaatccc cttgataaga gtttatcttg aaatcaatgg caaaaacaga gagtggaaga
     5401 aggcaaaaca agcctaacac cagtaagaaa aaagtttttt tcatcaacta agcccccttt
     5461 ttatctttct tatcattata gcattttttc gtaagtaaag gcttacttat gttgaaaaat
     5521 tacgcatttt tcgataaaat aggagacagt tattttttaa tagatcagaa taggagaaat
     5581 catgagaaaa atatacttat ctattttcac aagtctcttg ctgatgctag gacttgtcaa
     5641 tgttgctcaa gccgatgaat atttacgcat cgggatggaa gcagcatatg ctccctttaa
     5701 ctggacccag gatgatgata gcaacggagc tgtcaaaatc gatgggacca atcagtatgc
     5761 caacggatac gatgttcaaa tcgccaagaa aatcgctaag gacttaggta aagaaccttt
     5821 ggttgttaaa accaagtggg aaggtctagt ccctgccctt acttctggta agattgacat
     5881 gattatcgca ggtatgagtc caactgcaga acgcaaacaa gaaattgcct tttcgagcag
     5941 ttactacact agcgaaccag ttttgcttgt caaaaaagat tctgcctacg caagtgctaa
     6001 atctttggat gactttaatg gtgcgaaaat cacttctcaa caaggtgtct acctctacga
     6061 cttgattgca caaatcccag gtgctaaaaa agaaacagcc atgggagact tcgctcaaat
     6121 gcgccaagct cttgaggctg gtgtcattga tgcttatgtt tctgaacgtc cagaagcact
     6181 gactgctgaa gctgcgaact ctaagttcaa gatgattcaa gtagaacctg gtttcaaaac
     6241 tggggaagaa gatacagcta tcgctatcgg gcttcgtaaa aatgacaatc gtattagcca
     6301 aatcaatgcc agcattgaaa ccatttcaaa agatgaccaa gttgccttga tggatcgtat
     6361 gatcaaggaa caacctgccg aagctacaac aactgaagag actagcagta gtttctttag
     6421 ccaagtcgct aaaattcttt ctgaaaactg gcaacaactc ttgcgtggtg ctggtatcac
     6481 tcttttaatc tctatcgtcg gaaccatcat aggtctcatt attggacttg ccattggtgt
     6541 cttccgtact gctcctctct ctgaaaacaa agccatttat ggccttcaaa aactagtcgg
     6601 ctgggttctc aatgtctaca ttgaaatttt ccgtggtacg ccaatgattg ttcaatcgat
     6661 ggttatctac tatggaactg cccaagcttt cggtatcaac cttgaccgta cactggctgc
     6721 tatcttcatc gtttcaatca ataccggtgc ctacatgact gaaatcgtcc gtggtggtat
     6781 cctagcagtt gacaagggac aatttgaagc tgcgactgct cttggtatga cccataacca
     6841 gaccatgcgt aagattgtcc tacctcaggt agtccgtaac atcctacctg caactggtaa
     6901 tgaatttgtc atcaatatca aagatacatc tgtattgaac gttatctctg ttgtcgaact
     6961 ttatttctca ggaaataccg tggcaacaca aacctatcaa tacttccaga catttacaat
     7021 catcgccgtg atttactttg tcctcacctt caccgtaaca cgtatcctac gatttatcga
     7081 gcgccgaatg gacatggata cctatactac aggtgctaac caaatgcaaa cggaggattt
     7141 gaaataatga cacaagcaat ccttgaaatt aaacacctca aaaaatccta tggacaaaac
     7201 gaagtgctaa aagacatttc actcactgtc cacaagggag aggtcatctc tatcatcgga
     7261 agctctggaa gcggaaaatc gaccttccta cgctccatta acctacttga aacaccaact
     7321 gatggacaaa tcctttatca tggacaaaac gtcctcgaaa aaggctatga cctcacgcaa
     7381 taccgtgaaa agttggggat ggttttccaa tcctttaacc tctttgaaaa tctcaatgtt
     7441 cttgaaaaca caatcgtcgc tcagacaact gtcctaaaac gcgaacgcac agaagctgaa
     7501 aagattgcca aagaaaacct ggaaaaggtc ggcatgggag aacgctactg gcaagccaaa
     7561 ccaaaacaac tctcaggtgg tcaaaaacaa cgtgtggcca tcgctcgtgc cctctccatg
     7621 aatccggacg ctattctctt tgatgaacca acatcagctc tcgatccaga aatggttgga
     7681 gaagtcctca aaatcatgca ggacctggct caggaaggct tgaccatgat tgtcgtaacc
     7741 catgaaatgg aatttgcccg tgatgtctct caccgtgtta tctttatgga taagggcgtg
     7801 atcgctgaag aaggtaaacc agaagacctc ttcaccaatc ctaaagaaga ccgaacaaaa
     7861 gagttccttc aacgctatct caaataaaaa gaaaaggctg catcaaccgt gcagtctttt
     7921 tgctgtcctc aaaatgaaaa ggcagattct ctatctctac tacttgaagg tcaagaatcc
     7981 gccgttatcc aaagattaat cttcaaattt ttcataattt ttttcataga aatcaattaa
     8041 gcctagggct gtttcaaatg aaatattttt tacttttgca cgaccctgcg ccagagcaat
     8101 gatagacatt tcacgcgcat tcgtttcttt agagatacgg tagcctgtga ttttcttatc
     8161 acgaacccag ccaacaactg attctacttt ttcaaagttt gacttagcca tgtttttctc
     8221 ctattttctt ctttataata tttaattgta tacgttttta ctacttttgt caataaaaaa
     8281 aacatatatt caacgaaaat aattagttca gaattttctt acttcctttt taaatccgat
     8341 aatatatagg ttttgctagc ctctacacca taaatatttg ctaataaaac tactgtatta
     8401 ttaccctctt aaggtgcgaa attattcaag ttcttaaaaa atgcttctct atttccgtaa
     8461 gatagcttat ttcttcgatt taagaactga agatgatagt tctaaccttt agaaatccaa
     8521 gccttcacta gctcaaaaac tcctatcttt cttcctcaat aaatcatctg agagataggt
     8581 atttcttata tccttcaaat tcatcactcc tttggatatt gaggtgctag cctagaagca
     8641 gacacccgat tatctaaacc atccttaata gagatgacaa gacagataag ctcagttaat
     8701 tacttatata tctttttaat atggtatatt tgtcaacaaa aagaaatcaa atcatacctt
     8761 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaagttgct caaaacactg
     8821 ttttgaggtt gcagatagag ctaacgtggt ttgaagagat tttcgaagag tattaaagac
     8881 agtgtgacta tgcttgtcaa tagataggaa atcaaaatga aggatggaaa tctgagccaa
     8941 acatcataag tgctataatg aacagaagta agaattgcac gataagtttt ctaaaaccat
     9001 aggaatcgtc ccctttcttc atcttcatta tagcatatat acatcagttg tacaaataat
     9061 atgatatttt tttattagtc ctcagacaag catattataa agcgtttcat taccatttaa
     9121 gttaatataa gctggatcaa aaccttccat tctacgaatc aaacctgcat aatcatgctt
     9181 atctgctaaa gcgatcccaa tcaatactgg gcctgttccc ttgctagctc gtttgatata
     9241 ctcaaaacgt gtgatatcat catttggccc caggatatca tttacaaact cacgcaaagc
     9301 tcctggacgt tgtgggaaat tgaccacaaa ataatgtttg ataccatcat aaatcaaggc
     9361 acgctcttcc atttctggca tacggttgat atcattattt cctccagaaa tgatacaaca
     9421 aatggttttc cccttaatat attcagctaa aacctctaaa gaggcgatac tagctgctcc
     9481 agcaggttct gcgactatcc cttgcttaga gtaaaggtca atcaaggttt cagaaatcaa
     9541 tccctcatcg acacctacca aagtttgaat atgttgacga gttgcttcat aggtcaactg
     9601 acctaccttt tgcacagcaa tcccatccgc aaatttatca atttccttga gtttgactgg
     9661 acctccagcc tcaaaggcag ctttcatgga acgcgctcca ttcgcctcta ctccgataac
     9721 ctcaatctct ggacttgttt ccttgatata ggtagaaacc ccggcaatga gaccgccacc
     9781 accaacagga accaagacag catcaaaatc aatcgattct tttcgagctt cttctaaaat
     9841 ctcataagca actgttcctt gacctgcttg aacatgagca tcatcaaaag gatcaataaa
     9901 ggtacgattt tcagagactg taaattcttg agctgctttg gctgaggcat caaaggtatc
     9961 tccaactagt ttaatagtta caaaatcccc accaaaaaag cgaacctgac caatcttttg
    10021 ttgtggcgta gtaatgggca taaagatagt agcaggaatt ttcatttcat tacaagtata
    10081 ggctactccc tgcgcatgat ttcccgcaga agcgcaaact accccacgtt cacgttcttc
    10141 cttgctgagc tgggaaatgg cataataggc accacgaatt ttaaaggagc gaacacgctg
    10201 ggcattttct tttttcaaat aaatcttagc accatacttc tccgataaat aatgatcgta
    10261 atccagtgga gtattcacaa ccacaccgtt caagaccttg tgagccttga ttatatcttt
    10321 tgaacttaac atataaaacc tctttttcta taaaaaacag tcactaatga ctgtttttta
    10381 gttattcatc tccgaaaatt tcagtaacac tttcatggta tttatgagcc atacgaatcg
    10441 aaaactcttt aggaagttca taacgatttg tggaaatcca atcatataac cattctacca
    10501 aactttcttc actatcatta cgatggaaga taaaatagtt taaaatttct ttatattcta
    10561 catcattcaa atccaacgtg tctacaaatt gactacgagg aacaaaatta gaatctaatt
    10621 ttcttagttg atctgtcaat tctcccaatt gtaaatccaa ctgaaaaaat agtttttgca
    10681 gttgcctatc agtttcatca catcctgtct tgatagaaaa caaattggta atattcatat
    10741 gatttcaacc cctaaataag tacatctaat atcccacgaa tagctgaagc taaagttttc
    10801 gcagtccagt catttaaacc cgattctttc aatccagatg caataatttg ctctatttga
    10861 tataaaccgc catctatcgt ctcatttatt ctgcgaagag gaccttcaat ataggataaa
    10921 gcttctccga atctaacagc tgatttaggt cctaatgatt taccaactat atttgttaat
    10981 attttttatt tctcaaaatc catttcgcta tataacgagc agatttagtc gcaccagctc
    11041 tctcaaacgg agaatgaatt tcaagacctt cttctctagc tatttgtgcc aatttgaaga
    11101 attgttcaaa ggtcatatgc aacctaccat cttcaaaatt agcatcatca taaatgttaa
    11161 ttacagcatt ttcatcatac gaccaatctg ctttattttg gtctgctagc actattaccg
    11221 acggtgtaat tacattatac aactagaacc aataataacg atgcaatttt tctcataatc
    11281 tttttcataa aatcatctcc agcgctatat atttctaatt agttatagat tttgaatgca
    11341 tcatcgtcgt ttttaccaac gaatggcatt gctttacgca attctgcacc aactttttca
    11401 atttcaaggt tagctgcttg ttcacggtaa gcagtcaatt ttggacgtcc agctttatag
    11461 tcatttacaa agtcatttgc aaatttacca ttttggatgt ctgccaagac agccttcata
    11521 ttttctttaa cttgttcagt gattacacgt ggacctgata catagtcacc gtattcagca
    11581 gtgtttgaaa tagattgacg cattttcttg aatccacctt cgtagatcaa gtcaacgatc
    11641 aatttcattt cgtgaagaac ttcaaagtaa gccaattctg gagcgtaacc tgcttctgtc
    11701 aagacttcga aacctgcttc gataagggca gtcaaaccac cacaaagtac agcttgttca
    11761 ccaaacaaat cttcttcagt ttcttctttg taagttgttt caagaagacc tacacgagcc
    11821 gctccaacac ctttacacca gtccatagca atgtttttag catttcctgt tgcatcttgg
    11881 tatactgcat aaagagctgg aacaccaaat ccttcttcgt aagtacgacg taccaagtgt
    11941 cctggtcctt taggagcaca catgaagaca tctacatccg caggaacttt gataaattca
    12001 aagtggatgt tgaaaccatg ggcaaatcca actgcgtttc cagcttccaa gtttggagcg
    12061 atttctgctt cgtacaattc ttgttgaatt tcgtctggcg ccaagatcat gataacatca
    12121 gccaacttag tagcttctgc tactgtgtaa gtatcaaatc catcttcttt tgctttatca
    12181 aaagatttac ctggacgtac accgataata acgtcacgac ctgaatcacg caagttttga
    12241 gcatgcgcat gcccttgtga accataaccg ataacggcga tttttttacc gtcaagtgct
    12301 gctactttaa catctttttc atattccatt tgaactgtca tagcttttct ctcttttcta
    12361 ttatttattg ccttttaagc tggtttaaca aatttaagtt ggatttttaa tcgcgggtaa
    12421 atccagttgc acccgttcga gcaatattgc gaataccgta tgggcgaatg actcgcaata
    12481 gggcttcgct cttttctgca tttcccgtca tctgaatggt aatcgagctt ggcgctacgt
    12541 ctaccactgt tgcacggaaa ggttgaataa tcgctaaaat ctcagctctc ttctcagctg
    12601 gcgctgacat cttaaccaaa atcacctcgc gctccaaatg aggcttgtct gtaatatctc
    12661 gaatgcgaat cacatcaatc tgacgattga gctgtttgat gatttgctcc acttcatcat
    12721 gagaagcaac atcaataata atagtgatac gcgatacatt cggatcttct gttgctccaa
    12781 cagagatgct ttcaatatta acctgacgac gagataggac acctgtaaag cgattgagga
    12841 ctcctgaacg attttgtagt tttgctgtta acattctacg catggaactg cacccccaac
    12901 atctcatgat tactcttacc agccggtacc attggtaaca cctgttcctt acgagaaata
    12961 tctacctcaa ttagcatagg aacatcctca gtgatgactt caaggtcttg agccaaggtc
    13021 tcaggattgt caaacttata gtttttaata ccataagcct gcgccatcaa ttggaaatca
    13081 ggaagggtat caaagaccga ctctgatgtt ctgccttcat agaaggattc ctgccactgg
    13141 cgaaccattc cgagtgaatg attgttcagc ataaccacct tgattggcac cttgtaaata
    13201 ttcaaaatag ccaactcctg gttggtcatt tggaaaccac catccccaac aaacaagact
    13261 acttccttat ctgggttagc aattttagca ccgattgctg ctggaattcc aaagcccatt
    13321 gttcccaaac cacctgaagt cactaactga cgttcatttt ggtagggata atactgagct
    13381 gtccacattt ggtgttgacc aacgtctgtt accacaatgg catctccatt cgtcaattca
    13441 ccaattcgtt caataactgc ttgcggttga accacacgct ctttcttgtc atacgaacga
    13501 acacgattct tgtctttagt gactttctca atccactttt cagtattgtt gtgaactgtt
    13561 ggttctgcta gcaacatttg caaggccttc ttagcatctc caactacagg aatgtctgca
    13621 ctgataatct tgccaatctc agctgggtca atatcaatgt gggcaacctt agcattctta
    13681 gcgaaagtct taggattccc cgtcaaacgg tcatcgaaac gagaaccaat actaatcata
    13741 aagtccgctt ccgtcatggc aatatttgct gcgaatgacc cgtgcatgcc tcccattcca
    13801 agaaagagtg ggtgactcgt tgcaatcgtt ccttgtccca aaagactggt taccactgga
    13861 atttgatagc gttctgcaaa ttcatttagt tccgtagcag cctcagcata actaattcca
    13921 ccaccagcta acaagactgg ctttttagcc ttggacaatt gcttcaagat tttcttgatt
    13981 tgcatatcat tcggctcaag agtcggctga taacttggta aattcacttc tggtgaataa
    14041 atgaagtctg tttctaaagc agatatgtct tttggtaggt caattacaac tggccctgga
    14101 cggcctgtag ttgcgatatg gacagcttcc gtaatgatac gcggaatatc agctgtctca
    14161 cgaacttggt aattgtactt agtgattggc atggtaattc ctacgatgtc tgcctcctga
    14221 aaggcatcct tcccaatccc tgctcgcgcc acctgacctg taaagaccaa aaggggaacg
    14281 ctatcgctca tagcatccgc aatccctgta atggcatttg ttgctcctgg tccactagtg
    14341 acgacggcaa cacccaactt tccagttgat ttggcatagc cttcagcttc atgcaaacaa
    14401 ccttgctcat ggcgccctag aatgtggcga atgcctttaa aattatatat cgcatcataa
    14461 aaaggcaaaa ccgcaccacc aggataacca aagatggtat caactcctaa atcacgaagt
    14521 gtttccaaaa ctaggtccga ccccgtctta ggagattcta aactgatttt ctccattatt
    14581 cccctttctt tcctcttaaa aataacttgt tactatcata ccatt
//
LOCUS       DAVLWA010000042        14525 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLWA010000042 DAVLWA010000000
VERSION     DAVLWA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.042"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(148..1410)
                     /locus_tag="VYN80_001246"
     CDS             complement(148..1410)
                     /locus_tag="VYN80_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1541157.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(1410..1991)
                     /locus_tag="VYN80_001247"
     CDS             complement(1410..1991)
                     /locus_tag="VYN80_001247"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1541158.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(2391..2486)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2870..3109)
                     /locus_tag="VYN80_001248"
     CDS             complement(2870..3109)
                     /locus_tag="VYN80_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1541159.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(3109..3252)
                     /locus_tag="VYN80_001249"
     CDS             complement(3109..3252)
                     /locus_tag="VYN80_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1541160.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(3264..4091)
                     /gene="xth"
                     /locus_tag="VYN80_001250"
     CDS             complement(3264..4091)
                     /gene="xth"
                     /locus_tag="VYN80_001250"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1541161.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            4225..4680
                     /locus_tag="VYN80_001251"
                     /pseudo
     CDS             4225..4680
                     /locus_tag="VYN80_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            4677..5207
                     /locus_tag="VYN80_001252"
     CDS             4677..5207
                     /locus_tag="VYN80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1541162.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     PEACRSVGLSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDG
                     VIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKPGVLYSS
                     KISSNRVNVALPYVGY"
     gene            5335..7077
                     /locus_tag="VYN80_001253"
     CDS             5335..7077
                     /locus_tag="VYN80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541163.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDA"
     gene            7070..8821
                     /locus_tag="VYN80_001254"
     CDS             7070..8821
                     /locus_tag="VYN80_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541164.1"
                     /translation="MHNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMPYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            8994..9686
                     /locus_tag="VYN80_001255"
     CDS             8994..9686
                     /locus_tag="VYN80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1541165.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            9729..10955
                     /locus_tag="VYN80_001256"
     CDS             9729..10955
                     /locus_tag="VYN80_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1541166.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            12119..14245
                     /gene="pfbA"
                     /locus_tag="VYN80_001257"
     CDS             12119..14245
                     /gene="pfbA"
                     /locus_tag="VYN80_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1541167.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILADEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPIEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNI
                     ELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYISFIKSNANGKE
                     PVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVIDSNQKNGEFNS
                     SKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKLPKTGSNKI
                     MELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            14229..14525
                     /locus_tag="VYN80_001258"
     CDS             14229..14525
                     /locus_tag="VYN80_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541168.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
BASE COUNT         4398 a         2735 c         3101 g         4291 t
ORIGIN      
        1 tctttctaat gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac
       61 acaaaaataa gctccataat atccataggg gatttaccca ctacaaatat tatagagcca
      121 caaaaaagag gctcgcacct ctttttctta tttcttttta tgatttaata cggcattgag
      181 gacaatagcg agtaggctgg ctacgacgat tccgtttgag aagaacattt ggaaggctgt
      241 cggcatgctg acaaagagat tactgttgtt gagaccgaca cctgcagcga ttgaaacagc
      301 tgcgataagg aagttgtgtt cattgttagc aaagtcaact cgggcgagga tttgcatccc
      361 ttgaatagat acaaaaccaa acatcaccag catggcaccg ccgaggacgg agcttggaat
      421 gatttgggca agggcaccaa acttaggaag cagtccaagg agaaccagga aaccagctgc
      481 gtagtagatt ggcaggcgtt ttttgatgcc tgacaattta accaaaccaa cgttttgtga
      541 aaatccggtg taagggaagg tgttaaagat tcctccgaga agtaccgcca aaccttctgc
      601 gcggtaaccg ttgcgaaggc gcgtgctgtc gattggatct tttgtgatat cagacaaggc
      661 tagataaaca ccagttgact caaccataga caccgttgcg atgatacaca tcatgacaat
      721 agatgagatt tcaaaggttg gcatcccaaa gtagagtgga gttgggacat ggacaagtgg
      781 agctaccgca acaggagaga agtccaccaa gcccatagta gcagcaatgg cagttccaac
      841 aaccagacca atcaaaatag agatagactt gataaatcct ttggtaaaga tgttgatcaa
      901 gaggataatc agaacagtaa tagctgaaag caagagactt tgaccagttg gctctggaac
      961 gttatttccc atatttccaa tagcgacagg gatcaaggtt aaaccaatcg tggtaataac
     1021 agatcctgtt acgatagagg ggaagagatt ggccactttt gagaagatgc ctgaaacaag
     1081 aaccacgtaa atcccagatg cgataagggc accaaacata gcgccactac catggctttg
     1141 cccaatcata atcaagggag cgaccgactg gaatgcaact ccaagaacga ctgggagtcc
     1201 aatcccaaag tatttgttga gttggagttg gaggaaggtt gccaccccac acatgaagat
     1261 atctgtagaa atcaggtagg tcaactgctc agctgaatag ccaagggctg tcgcaatcat
     1321 gatgggaacc aggatagatc ctgagtacat ggctagtaag tgctgcaagc caagaacggc
     1381 tgcttgcgag tgtttttctt gagtttgcat tagagatctg cctccttaaa tacgacctga
     1441 ccattttcaa aacgatccaa acgagcaagt gataggacag ggtagcctgc tttttcaagc
     1501 aaatcacggc catcttggaa ggatttctca atcacgatac cgatagcttg gactgtggca
     1561 ccggcctgtt cgatgatttg aatcaagcct ttagcagctt ggccattagc aaggaaatcg
     1621 tcgataatca aaaccttgtc ctctggtgag aggaattttc cagcgataga aacggtgctg
     1681 gtcacctgct tggtaaagga gtagacttga gcagttaaga tgccttcgtt catggtgatg
     1741 ttcttagctt ttttggcgaa aatcatggga acgtttaagg cttcagctgt aaaaacggct
     1801 ggggcaatac ccgacgcttc aatggttacg accttggtaa tgccggcagc agcaaatttt
     1861 tccgcaaaaa ccttaccaat ctctcgcatc aagctaaagt caacttggtg ggttaaaaag
     1921 gaatctacct tgaggatgtt atcacccaag atatgcccat ccttgaggat gcgctcttct
     1981 aataatttca taagacctcc taaagtctaa aagttaattt acttgttgtt taaatgtttc
     2041 tatagtgatc ccttttgcta atgctatata tttgataaaa ctattacgag cgaagcgagt
     2101 cttatcaaat attttccgtt gtagtggtat catagacaat aatcttgtta ttgtctatga
     2161 cgggattttt gagagtaaaa tagttcgggg aactatttta gcctaagcct agaaatgaaa
     2221 gagctagggg ctcaaaaatt agggatgaaa ttccctggat tcctgaaatt attcacagga
     2281 taatttcacc tcccgtccgc actaattaag ggaaatatta aaaatattca aaaaagacct
     2341 acttaatctc taagtaagtc ccccaaatag gcatggcaaa aacggccata tctcactgct
     2401 gacttactta ttgttaggtg ttccggcacc ttgtagaaac gtcgtgccaa ttcacgacat
     2461 aaacaagtaa aacgatattc aattttaaat aggcttgagc caatgttttt attttacact
     2521 aaataacttt agaaatcaac tattttgtta gtgttttggt ttaaaaaacg aacaaacaga
     2581 agagagggtg aacaaaaact ccattgtaag ctaacattta tactaaatga aaatcaaaga
     2641 gcaaactagg aagctatcca caacctcaaa acactgtttt gaggttgtgg atagaattga
     2701 cagagtcagt attatatacc tacggtaagg cgacgttgac gtggcttgaa gagattttcg
     2761 aagagtatta gaagattttt ccatcataaa aggcatacta tcaagttttt agacacctga
     2821 caatatgcct ttttctaact tttaaagact tttcccaatt tttattattc tactcgctaa
     2881 atcttaaaaa atagccatct ggatccaaaa ctgcaaattt atgaggatag atatagggat
     2941 cactgacacg aaactttctt ttggtcaagg gacgataaat aggatagttt gcctccatca
     3001 ctttttgata gagttttgga acatccttta tgccaaagga gagattgact ccacgaccaa
     3061 aggggtaggt cagttcagct agttgatcct ttgttccctc ctctaacatt agttgacact
     3121 cttcaagaga aagagaaagt tttcttctgg acgttggtat tcaatcctaa aacccagtaa
     3181 accacagtag aaggaccggg actgttcgat attcgataca agaaactcgg gaatgaccgc
     3241 attgtagtcc atatagaaaa tccttacaag tcaatttcca agacaatcgg tgtatggtct
     3301 tggcgagcac ctgagtcaat catatcagat ttagtgacct tgtcagcgat acggttactt
     3361 gtgagccagt agtcgattct ccagcctgta ttgttgattt tagaagtttt gctgcgttgt
     3421 gcccaccaag tgtagcgttc aggaacatcg ccatgaacat ggcggaaggt gtctgtaaat
     3481 ccagttgcca aaaggttggt aaatccagca cgttcctcgt cagtaaatcc aggtgaacgg
     3541 cggttgctag caggatttgc aaggtcgatt tcattgtggg ctacgttgta gtcaccggtc
     3601 gcaaggactg gtttttcttt gtctagttca gccaaatact cagcatattt aacatcccag
     3661 acttggcgtt cttccaagcg tttgagaccg tcaccagcgt ttggagtgta aacttgggtt
     3721 acgaaaaatg catcaaattc tagagtgatg atacggcctt ccaagtccat ggtagaaggg
     3781 gcaccgattt ctggaaaact gatcgtaggc gtaagttctt tcttataaag gaacatggtt
     3841 ccagcatagc ctttacgggc aggctcttgg gaagagcgcc atgtgttttc gtagcctggg
     3901 aagagttctt ctaaaatttc cacgtgtttc tttgtaggtc ctttggcaga aagcttggtt
     3961 tcttggatag caatgatatc agcattttca gcgaccaagg tttgtaggac ttcttgggac
     4021 aatttggcac gagctgagtc actagttagg gcagcgttta gggaatcaat attccatgag
     4081 ataagtttca taaagttacc tttttcattc agattataga ttttattata ccaaaaaaag
     4141 atctatttcc ccaacgtatg gtttgaaaaa ttactctctt tcgtttataa ttaagaatga
     4201 ttttatgaaa gggagtgaaa atatatgaaa ttctactctt atgactatgt actcagccaa
     4261 atcggtcagc aaaatggtat catggttggc tttgggattg ttctattagc tgtgacagtt
     4321 tttttgcttt caaggcatac catgataaaa agggaaccga atttcgtgag ttggtcatga
     4381 tttcagctct ggccttattt agctctgctt ttggtcagca tcacgactta tcaaaacaat
     4441 caagtttcta acaataaatt tcaaacttca cttcatttca tcgaggttgt ttccaaagat
     4501 ttgggagtag acaagtcaga agtctatgtt aatacttcca caaacacaga tggcgcactt
     4561 atcaaggtgg gagatcgcta ttatcgtgcc ctaaacggaa gtgagccaga caagtacctg
     4621 ttagagaaag tcgaattgta taagacagac gcaattgaac tggtggatgt gaacaaatga
     4681 cacttaatta tatcgaaatt ttaatcaaac tggtcttgac tctcaaatgg ctcaacaaca
     4741 atgttcactt tgtgaaacgt ttgattgatg gtaagccaac tctccttatc aaaaatggga
     4801 atattgaccc agaagcctgt cgttcagttg gtttgtctgc atcggatgta gccctcaaac
     4861 ttcgtagcca agggattttc cagatgaagc aagtcaaacg agctgtgcaa gagcaaaatg
     4921 ggcaacttat cgttgtgcaa atgggagatg aaaatcctaa gtatccagtt gtgactgacg
     4981 gtgtgattca agtagatgtc ttggaatcga ttggtcgtag cgaagagtgg ttgcttgata
     5041 acctcagtaa acaagggcat gacaatgtag ccaatatctt tattgctgaa tatgacaagg
     5101 gtgctgttac agtcgtaact tatgaaaaag aaaaacctgg ggtcttgtac tcttcgaaaa
     5161 tctcttcaaa ccgcgtcaac gtcgccttgc cgtatgtagg ttactgactt cgtcagttct
     5221 atctacaacc tcaaagcagt gctttgagca gcctgcggct agtttcctag tttgctcttt
     5281 gattttcatt gagtattggc ctcaggtttc catttgcaat cagaaaggga ttttatgtcc
     5341 attattcaaa aactttggtg gtttttcaag ttagaaaaac gccgttatct agtcggaatt
     5401 gtggccctgg tcttggtttc cgtcctcaat ctcattcctc ctatggttat ggggcgggtt
     5461 attgatgcca tcacatcggg gcaattaacc cagcaggacc tccttcttag cctattttac
     5521 ttgctacttg cagcctttgg tatgtactat ttgcgctatg tgtggcgtat gtatatcctt
     5581 gggacctctt attgcttggg acagatcatg cggtctcgct tgtttaagca tttcacaaaa
     5641 atgtcgtcag ccttttatca aacctatcgg acgggtgatc tgatggcaca cgcaaccaat
     5701 gatatcaatg ccttgactcg tttagcaggt ggcggtgtca tgtctgcggt ggatgcctct
     5761 atcacggctc tggtgacttt gttgaccatg ctctttagca tctcatggca gatgactctt
     5821 gttgccattc tccccctacc tttcatggcc tatgcgacta gtcgcctagg gagaaagact
     5881 cataaggcct ttggcgaatc ccaagctgct ttttctgaac tcaataacaa ggtacaggag
     5941 tccgtatcag gtatcaaagt gaccaagtct ttcggttatc aggcagacga gttgaagtct
     6001 tttcaggcag tcaatgaatt aaccttccaa aagaacctgc aaaccatgaa atatgatagt
     6061 ctctttgacc ctatggttct cttgtttgtt ggttcgtcct atgttttaac gcttttggtt
     6121 ggctccttgg tggttcagga agggcagatt acagttggga atctagtcac ctttatcagc
     6181 tatttggata tgctggtctg gcctcttatg gccatcggct tcctctttaa tactactcag
     6241 cgagggaagg tttcttacca gcggattgaa aatcttttgt ctcaggaatc tcctgtacaa
     6301 gacccggagt ttcctctgga tggtattgaa aatgggcgtt tggagtatgc cattgacagc
     6361 tttgcttttg aaaatgagga aacactgacg gatattcact ttagtttggc aaaagggcaa
     6421 acactgggct tggttgggca gacaggctct gggaaaacgt ccttaatcaa gctcctcttg
     6481 cgtgaatacg atgtggataa gggcgccatt tatctaaacg gtcacgatat tcgggactat
     6541 cgtctgacag accttcgcag tctcatgggc tatgttcctc aggaccagtt tctttttgcg
     6601 acttcaatcc tagacaatat ccgctttggc aatcctaact tgcccctttc agcggtcgag
     6661 gaagctacta agctagcccg agtttaccaa gatattgtag acatgcctca aggatttgat
     6721 acgctgattg gtgaaaaagg agtcagtctt tctggtggtc aaaagcaacg gttggctatg
     6781 agtcgggcta tgattttaga ccctgatatc ttgattttgg atgattcctt atccgccgta
     6841 gatgccaaga cagagtatgc gattatcgac aacctcaagg agacgcgaaa ggacaagaca
     6901 accattatca ctgcccatcg cctgagtgca gttgtccatg cagatttgat tttagtcctg
     6961 caaaatggtc aaattatcga acgaggcagg cacgaagact tgctagcttt ggatggctgg
     7021 tatgcccaaa cctaccagtc tcagcagctg gaaatgaagg gagaagaaga tgcataataa
     7081 acaagaacaa tggactgtat tgaagcgctt gatgtcctat ctcaagcctt atggactcct
     7141 tacctttttg gcactcagtt ttctcctagc gacgacggtc attaaaagtg tcattcccct
     7201 tgtggcttcc cactttatcg accagtatct cagcaatctt aaccaactag ccgtgaccgt
     7261 tttgctggtc tactatggtc tctacatcct acaaactgta gttcagtatg tcggcaatct
     7321 tctctttgcg cgcgtgtctt acagtattgt tagggatatt cgtcgggatg cctttgccaa
     7381 tatggagaaa ctgggcatgt cttactttga caagacgcca gcaggttcta tcgtttctcg
     7441 tttgaccaac gataccgaga cgattagtga tatgttttct gggattttat ccagctttat
     7501 ctcagcagtt tttatctttc tgacaaccct ttataccatg ttggtgctgg attttcgttt
     7561 gacagcatta gtcttgctct ttctcccctt gattttcctt ttggtcaatc tctatcgaaa
     7621 aaagtcagtg aaaatcatcg agaaaaccag aagtctcttg tcagatatca atagtaagct
     7681 ggcagagaat atcgagggaa tcaggattat tcaggctttt aatcaagaga agcgcctgca
     7741 ggcagaattt gatgaaatca accaagagca cttggcctat gccaaccgtt ctgtagcctt
     7801 ggatgccctc tttttgagac ctgccatgag tttgttgaaa cttctaggtt atgctgtctt
     7861 gatgccctat tttggctatc gtggtctttc tatcgggata acggccggga ccatgtatgc
     7921 ttttatccag tacatcaacc gcctctttga ccctttgatt gaggtgacgc aaaacttttc
     7981 aacactgcaa acgtctatgg tttctgcagg ccgtgtcttt gccttaatcg atgagaggac
     8041 ctatgagccc cttcaagaaa atgggcaagc caaagtcaaa gaaggcaata tccgttttga
     8101 acatgtgtgt ttctcgtatg acggtaaaca tccgattctg gatgacattt ctttctctgt
     8161 taataagggt gaaaccattg cctttgtagg tcatacaggt tcagggaaat cgtctattat
     8221 caatgtcctc atgcgctttt atgaattcca gtcagggaga gttctcttgg atgatgtgga
     8281 tatcagggat ttcagtcaag aagagctgag aaaaaacatc ggtttggttt tacaggaacc
     8341 cttcctctat catggaacta ttaagtccaa tatcgccatg taccaagaaa tcagtgatga
     8401 gcaggttcag gctgcggcag cctttgtgga tgcagattcc tttattcaag aacttcctca
     8461 ggggtacgac tcccctgttt ccgagcgtgg ttcgagcttc tctactgggc agcgccagct
     8521 tcttgccttt gctagaacag tcgccagcca gcctaaaatc ctgattttgg atgaagcgac
     8581 agccaatatt gactctgaaa cagaaagttt ggttcaagct tctctggcga agatgagaca
     8641 gggccgaaca actattgcta tcgctcaccg cctttctact attcaagacg ccaactgcat
     8701 ctatgtcttg gataagggac gcattatcga gagtggaacc catgaggaac tcttgactct
     8761 gggaggaacc tatcacaaga tgtatagttt gcaggcaggg gccatggcct atactctttg
     8821 aaaatctctt taaaccatgt cagctttatc tgcaatctca aagctgtact ttgattttca
     8881 ttgagtacta gaaggaaatc cttcaaatta cagatttctt tcaccgcctt ttccattttg
     8941 tggtataatg aaaaatgttg acaaatagta taataaaaac aaaggagaac agcatgctga
     9001 aatgggaaga cttgcctgtg gaaatgaaat caagcgaggt tgagtcttac taccagcttg
     9061 tctctaaaag gaagggttcg ctgattttca agcgttgctt ggactgggtt ttggccttgg
     9121 tcttactggt tctaacttct cccatctttc tcatcttgag catttggatc aagttggata
     9181 gcaaggggcc agtgatttac aagcaagagc gtgtgaccca gtacaaccgt cggttcaaga
     9241 tttggaagtt ccgtaccatg gtgacggatg cggataaaaa aggaagtctg gtgacttctg
     9301 ctaacgatag ccgtattacc aaggttggaa atttcatccg acgtgtccgt ttggacgaac
     9361 tgcctcagtt ggtcaatgtc cttaaaggtg agatgtcctt tgtcggtaca cgacctgaag
     9421 tgccacgtta tacagagcag tatagccctg aaatgatggc aaccttgctc ttgcaagcag
     9481 gaattacctc tccagccagc atcaactaca aggatgagga caccatcatc agtcaaatga
     9541 cggagaaagg tctgtcagtt gatcaggcct atgtggagca tgttcttcct gaaaagatgc
     9601 gctataacct cgcctatctc cgagagttta gtttctttgg ggacatcaaa atcatgtttc
     9661 aaaccgtgtt tgaggtacta aaataaagta gtcataagaa aatgagtaca gataaaagga
     9721 gcaaatcaat gccaaattac aatattccat tttcaccgcc tgatatcaca gaagcagaaa
     9781 ttgctgaagt agcggatacc ctgcgttctg gttggatcac aacaggtcct aaaacaaaag
     9841 aactggagcg ccgcttgtct ctttacacac agacacctaa gactgtttgt ctcaactctg
     9901 cgacagccgc tctggagttg attttacgcg ttttggaagt gggacctggt gatgaagtca
     9961 tcgttccagc catgacctat acggcttcat gtagtgtcat tacgcacgtg ggagcaaccc
    10021 ctgtcatggt ggatatccaa gcagatacgt ttgagatgga ctatgacctg cttgagcaag
    10081 ctatcactga gaaaactaag gtgattatcc cagtagagct cgcagggatt gtttgcgatt
    10141 atgaccgttt gttccaagtc gtggagaaaa aacgtgactt ctttaccgct tcaagcaagt
    10201 ggcaaaaggc ctttaaccgt attgtcattg tctctgatag tgcccacgct ttgggatcta
    10261 cttataaagg acaaccttct ggttctatcg ctgattttac ttccttctca ttccatgccg
    10321 ttaagaactt tacaacggca gaaggtggaa gtgcgacttg gaaagccaat ccagtgattg
    10381 atgacgaaga gatgtacaag gaattccaaa tcctttccct tcacgggcaa actaaggatg
    10441 ctcttgccaa gatgcaactg gggtcatggg aatacgatat cgttacacca gcctataagt
    10501 gcaacatgac cgatatcatg gcttcacttg gtttggtaca attggaccgc tatccaagtt
    10561 tgttgcaacg ccgtaaggac attgtggacc gctatgatag tggttttgca ggttctcgca
    10621 tccatccttt ggcacacaag actgaaactg tcgaatcttc acgccacctc tacatcaccc
    10681 gtgtagaagg agcaagccta gaagaacgca acctcatcat ccaagaattg gctaaagcag
    10741 gaattgcaag taatgttcac tacaaaccgc ttcctctctt gacagcctat aagaatcttg
    10801 gatttgatat gacgaactat cctaaggcct atgccttctt tgagaatgaa attaccctcc
    10861 ctcttcatac taaattaagc gatgaagaag tagactatat cattgagact ttcaaaacag
    10921 tttctgaaaa agtgctaact ttatcaaaaa aatgacaaac tacagtcaag cgaaagtgat
    10981 cctgtcccta aaaagtctaa ttgagtgtaa aaactgttgt tttcaattga taatagttta
    11041 cacctgtagt tgaggcctct ttctcctcag agagagaatt tttataggat tttcctttct
    11101 tgtgggagtc ccgtggtttg aaataagatg tgagcaattt ggtgtagcat ttagaatcct
    11161 tactagacat catttagaaa atctagtgtc ttgttctagt tttcaattca ccctattttt
    11221 tgaaagacgt gagtttccat gagtgagatt gtggaaactc gcgtcttttt ttgttttcag
    11281 aatattgttc aaagttttgt gcctgtcttt catgttctat tcattctttt gcatgataga
    11341 atttatagca tgttgatatt ataataatac gaatattcca tatgtttagt gatgcttgct
    11401 atacatcatt agatctcctg cgagacaatc tataaaacac ttgtctacga ttacctatat
    11461 gccctattcc agtattttag aagcactgca tctattttta tcgaggttaa atctagcttt
    11521 tatagaaggt ctatttaaga aatatattgt agtgttttag tttcaatccg ccatatgagc
    11581 gatattcagg taaatatccc tggcgaatgc ttgtatgaca aggtatttgt tctttcattt
    11641 ataatttaca acatatcaat aaatttaaat atagtaaatg ggatatttta tattcaagct
    11701 aagaaagata gcatcacttt tgaatggaag gctaaagagc aaactaggaa gttggccata
    11761 gatagctcaa aaccctgctt tgaggttgta gatatagtaa aatgaaataa gaataggaca
    11821 aattgatcgg gacagttaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt
    11881 atagtttcag tctactatag aactgaccaa gtcagtaacc tagacttagg gcaaggcggc
    11941 actgacctag tttgaagaga tttccgaaga gtataaattt taatattttc ttgtgttatt
    12001 ccttgacaat tcaatttgga aaatatatga taaagataat gacagcggtg tcactctatc
    12061 tattttaaga aaagtaataa tcaattgtta aaaaatagta aaaaaattgg aggttctgat
    12121 gaaatatttt gttcctaatg aggtattcag tattcgtaaa ttaaaggtgg ggacttgctc
    12181 ggtactattg gcaatttcaa ttttgggaag ccaaggtatt ttagcggatg aagttgttac
    12241 tagttcttca ccgatggcta caaaagagtc ttctaatgca attactaatg atttagataa
    12301 ttcaccaact gttaatcaga atcgttctgc tgaaatgatt gcctctaatt caaccactaa
    12361 tggtttagat aattcgttaa gtgttaatag tatcagctct aatggtacta ttcgttccaa
    12421 ttcacaatta gacaacagaa cagttgaatc tacagtaaca tctactaatg aaaataagag
    12481 ttataaggaa gatgttataa gtgacagaat tatcaaaaaa gaatttgaag atactgcttt
    12541 aagtgtaaaa gattatggtg cagtaggtga tgggattcat gatgatcgac aagcaattca
    12601 agatgcaata gatgctgcag ctcaagggct aggtggagga aatgtatatt ttcctgaagg
    12661 aacttattta gtaaaagaaa ttgttttttt aaaaagtcat acacacttag aattgaatga
    12721 gaaagctaca attctaaatg gtataaatat taagaatcac ccttccattg tttttatgac
    12781 aggtttattt acggatgatg gtgcgcaagt agaatggggc ccaatagaag atattagtta
    12841 ttctggtggt acgattgata tgaacggtgc tttgaatgaa gaaggaacta aagcaaaaaa
    12901 tctaccactt ataaattctt caggtgcatt tgctattggg aattcaaata acgtaactat
    12961 aaaaaatgta acattcaagg atagttatca agggcatgct attcaaattg caggttcgaa
    13021 aaatgtatta gttgataatt ctcgttttct tgggcaagcc ttacccaaaa cgatgaagga
    13081 tgggcaaatc ataagtaagg agagcattca gattgaacca ttaactagaa aaggttttcc
    13141 ttatgccttg aatgatgatg ggaaaaaatc tgaaaatgtg actattcaaa attcctattt
    13201 tggcaaaagt gataaatctg gggaattagt aacagcaatt ggtacacact atcaaacatt
    13261 gtcgacacag aacccctcta atattaaaat tttaaataat cattttgata acatgatgta
    13321 tgcaggtgta cgttttacag gattcactga tgtattaatc aaaggaaatc gctttgataa
    13381 gaaagttaaa ggagagagtg tacattatcg agaaagcgga gcagctttag taaatgctta
    13441 tagctataaa aacactaaag acctattaga tttaaataaa caggtggtta tcgccgaaaa
    13501 tatatttaat attgccgatc ctaaaacaaa agcgatacga gttgcaaaag atagtgcaga
    13561 atatttagga aaagtatcag atattactgt aacaaaaaat gtaattaata ataattctaa
    13621 ggaaacagaa caaccaaata ttgaattatt acgagttagt gataatttag tagtctcaga
    13681 gaatagtata ttcggtggta aagaaggaat tgttattgag gattcaaagg gtaaaataac
    13741 cgttttaaat aaccaatttt ataatttatc cggtaagtat atatcattca tcaaatctaa
    13801 tgcaaacggg aaagaacctg ttatacgtga tagcgatggt aatttcaata ttgtaacgga
    13861 gaatgggctt tacaaaattg taacaaataa tttaagtgat aaaaacgaaa aagaaaaaaa
    13921 caaagaggaa aaacaatcta attcaaataa tgtaattgat agtaaccaga agaacggaga
    13981 gtttaactca agtaaagata atagacaaat gaatgacaag atcgacaata aacaagataa
    14041 taagacagaa gaagtaaact ataaaatagt tggagatggc agagaaactg aaaatcatat
    14101 taataaatct aaagaaatag tagatgtaaa acaaaaatta ccaaagactg gttcgaacaa
    14161 gattatggaa ctattcttaa cagtgacagg aattggttta cttttgacac taaaagggtt
    14221 gaagtattat ggtaaagata aataaaattt gttcgataca aggaagttcc gtagaaaatg
    14281 aggatattgt aggttcacaa aatcagtatt tttggattat tggtggagct acagatttat
    14341 ataattctaa agaggagata ggttattcag tctcagaagt tgtacatatt ttatcagaaa
    14401 gtttgtcggt taattgtaaa gaatcaaaaa ctcttaaaca aatatttgaa actgctttac
    14461 tcgaggttaa agatgaaatc ggtttaaact catatgaatt aacagagtat aataaaatga
    14521 aataa
//
LOCUS       DAVLWA010000043        14520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLWA010000043 DAVLWA010000000
VERSION     DAVLWA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.043"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            267..560
                     /locus_tag="VYN80_001259"
                     /pseudo
     CDS             267..560
                     /locus_tag="VYN80_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            667..1018
                     /locus_tag="VYN80_001260"
                     /pseudo
     CDS             667..1018
                     /locus_tag="VYN80_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1294..2600
                     /locus_tag="VYN80_001261"
                     /pseudo
     CDS             1294..2600
                     /locus_tag="VYN80_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2640..3650)
                     /gene="tsaD"
                     /locus_tag="VYN80_001262"
     CDS             complement(2640..3650)
                     /gene="tsaD"
                     /locus_tag="VYN80_001262"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1541169.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3640..4077)
                     /gene="rimI"
                     /locus_tag="VYN80_001263"
     CDS             complement(3640..4077)
                     /gene="rimI"
                     /locus_tag="VYN80_001263"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1541170.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4074..4757)
                     /gene="tsaB"
                     /locus_tag="VYN80_001264"
     CDS             complement(4074..4757)
                     /gene="tsaB"
                     /locus_tag="VYN80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1541171.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            4935..5120
                     /gene="cibA"
                     /locus_tag="VYN80_001265"
     CDS             4935..5120
                     /gene="cibA"
                     /locus_tag="VYN80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1541172.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5122..5274
                     /gene="cibB"
                     /locus_tag="VYN80_001266"
     CDS             5122..5274
                     /gene="cibB"
                     /locus_tag="VYN80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1541173.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6092..6325
                     /locus_tag="VYN80_001267"
     CDS             6092..6325
                     /locus_tag="VYN80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1541174.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6327..8006
                     /locus_tag="VYN80_001268"
     CDS             6327..8006
                     /locus_tag="VYN80_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1541175.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            8060..8152
                     /locus_tag="VYN80_001269"
                     /pseudo
     CDS             8060..8152
                     /locus_tag="VYN80_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8359..10272)
                     /gene="mnmG"
                     /locus_tag="VYN80_001270"
     CDS             complement(8359..10272)
                     /gene="mnmG"
                     /locus_tag="VYN80_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1541176.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10282..10737)
                     /locus_tag="VYN80_001271"
     CDS             complement(10282..10737)
                     /locus_tag="VYN80_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1541177.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10878..11999)
                     /gene="mnmA"
                     /locus_tag="VYN80_001272"
     CDS             complement(10878..11999)
                     /gene="mnmA"
                     /locus_tag="VYN80_001272"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1541178.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            complement(12400..14493)
                     /gene="pspA"
                     /locus_tag="VYN80_001273"
     CDS             complement(12400..14493)
                     /gene="pspA"
                     /locus_tag="VYN80_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV1541179.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKAYEEAKKKAEDAQKKYDEDQKKTEEKAENEKKAAADLNEATEVH
                     QKAYVRYFEIQRAKDSKKYKNNRDKYNKDLAEADQKIKDTKTVLDEKQSKFYAVRAVV
                     VPEAKELAVTKQKAEETKKGAEVAKEKYDKAAQEVEVAKKEVEAEEAELDKKVAELQN
                     KVADLEKEIADAEKTVADLEKEVAKLEKDVEGFKESDGEYAKFYLEAAEKDLATKKAK
                     LAEAKIKAATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEA
                     LQNQVAELEEELSKLEDNLKDAETNNVEDYIKEGLEEAIATKQAELEKTQKELDAALN
                     ELGPDGDEEETPAPEAPAEQPKPEKPAEETPAPAPKPEKSADQQAEEDYARRSEEEYN
                     RLTQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGS
                     WYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYN
                     DSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWAK
                     VNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVN
                     TTVDGYKVNANGEWV"
BASE COUNT         3977 a         3363 c         2859 g         4321 t
ORIGIN      
        1 tatttttcca caactatatt gcattgtaac catctcctta aacgacgcat tatgatattt
       61 gatagagaaa tttttatgaa taactcaata attttatagt aaatcatgct tatatctcaa
      121 agatacctat tttctcttgt ctcgaccttc tccaaagaat tgctataata ctattacaaa
      181 tacatctgca ctacacttca aattttagca ctgtataaaa acgtttcaac acactaactt
      241 taagaaaact tccactatta attgaaaaag ttgatagaga taaattaaag atctatattg
      301 aaactcatcc cgatgcttat ttgactgaaa tagctgctga attcaactgt cctccaacaa
      361 ctattcatta cgctctaaag gctatgggat atagtctaaa aaagagctgt acctactgcg
      421 aacaagaccc agaaaaagta aatcggttcc ttaaagaatt gaatcactta agctacctga
      481 ctcctattta tatttatgag acaggggttg agacctattt ttatctcgaa tatgatcgag
      541 ccttgagcag gcagttagtc tctctggaag aagatataat tatttgaatt aagatcgaga
      601 caacgcacac cagagattgc gatactgtta tagaagtact aatgcccttt tttgtttcaa
      661 tatactatgg ctccgatgac ctataaagat acgatgacga gtgacttttt cgaagcttgc
      721 ttccaaaaat tcttactacc tactttagat acaccatccc ttatcattat ggacaatgca
      781 aggtttcaca gaatgaacat gtgtaaggag cagggcatag actgttacca cttcctacct
      841 attcacccga gtataatccc attgagaaaa tatgggctta catcaaaaac atctcagaat
      901 aatattgtca aattacgatg cttttcttga ggcacttttg tcctattctt gtttcagccg
      961 actatactcc gttattgggc agctacggaa cagtcgatgg gacgatgggg ggacataaaa
     1021 aaatcctcca gttttgtttt ttataacagt atactggaga attgacaatc tcggtagata
     1081 cctcgttata gcgcggttac ttattaggca gttacaaaac aactgtgaac agaaaacatt
     1141 ccagagtcag acaagacttt ggaatgtttt ggctctataa tttctgtagt gggtaatccc
     1201 accccaggaa ttatagggtc gtttcttgta gaaaaaaagc cccatatgac ctataatgaa
     1261 aagcgtctaa ccaactcatt agaaagggtt catatggaac aacttaagaa taccacagat
     1321 ttgctcggat tgaaagacaa aaatatcaaa atcttgtctg ttctgaaata ccaaacccat
     1381 ctagtcgttc aggcaaagtt ggattccccc gctcctcctt gtcctcattg tcaagggaag
     1441 atgatcaaat acgacttcca gaaagcctct aaaattccgc ttctcgactg tcagggttta
     1501 cccacggtac tacatctcaa aaagcgccgc tttcagtgca agaattgcct taaggtggtc
     1561 gtttctcaaa catccattgt caagaaaaat tgccagattt ccaacatggt gagacaaaaa
     1621 atcgctcagc tcctccttga aaagcagtct atgactgaga ttgcccacag attggcggtc
     1681 tcaacttcca ccgtcatccg aaaactgagg gaatttaagt ttgaaaccga ttggaccaag
     1741 ttgccaaaag ttatgagttg ggatgagtat agcttcaaaa agagcaaaat gagcttcatt
     1801 gcccaagatt ttgagtccaa atccatcctc gcaattttag acgggcgaac tcatgcggtg
     1861 attcgaaacc atttccaacg ctatcagaga gaggttcggg agctggtcga ggtcatcacc
     1921 atggacatgt acagccctta ttatcggctc gctaagcaac tctttccaaa ggcgaagatt
     1981 gttcttgacc gcttccacat tgtccaacat ctgagccgag ctatgaaccg agtacgaatc
     2041 caaatcatga accaatttga ccgaaaatcc ttggagtatc gggcgctcaa gcgcttttgg
     2101 aaccctcgct ttttcgtttc taggctcggg ctaaatcagt ccactggact gatttactac
     2161 accaggatag cttcaagctc tgtcagaaac gattctatca gcccacgttt cgaatgcact
     2221 taacccatcg ggaagtacga gataagctgc tttcttactc tgagggatta caggttcact
     2281 acgaactcta tcaactcctg ctctttcatt ttcaagagaa gaatgccgac catttctttg
     2341 gattgattga gcaagaactg ccaacggttc atccgctttt tcaaacggtc ttttggactt
     2401 ttttaaggga tagagataag attatcaacg cacttaagct gccttattcc aacgctaaac
     2461 ttgaagcgac caataatttg attaaggtta tcaagcgcaa agcctttggt ttccggaact
     2521 ttaacaattt taaaaaacgg attttgatga ctttgaacat caaaaaagag agtacgaatt
     2581 tcgtactctc cagattgtag cttttcgcct acccactaca cttgacaaag agccactctt
     2641 tattccatgg tatcaaaggc aagacttggt ttggcattga ggtcccagcc tgcgaagttt
     2701 tctttgttcc actcgctgac gctggcatag gcaatcatac ctgcattgtc tccgcagagt
     2761 cgcagagggg ggatgataac cttgacatct gtgatttcgg ctgctaggcg ttctctgaga
     2821 cctttattgg ctgccacacc acctgccaca actagggttt taacaggata tttctccaaa
     2881 gccttcttgg tttttgccat gagaatgtcc ataactgctg cttggaagga agcacacaaa
     2941 tcttctgtag acaggctttc tcccttttgc tcggcattgt ggtgaagatt gataaaggca
     3001 gatttcaaac cagagaatga aaactccaga ttatcttcct taatcatagc acgagggaaa
     3061 tcataaatat cctgcccctg atgagccagc tcgtcaatct cacgacctgc aggataggtc
     3121 aagcccatga cacgaccgac cttgtcatag gcctcaccaa ccgcgtcatc tcgcgtttcc
     3181 ccaacaatct tgtaatcgcc agcctcagaa acatagacca actctgtgtg cccaccactg
     3241 actaaaaggg ctagcaaggg aaactccaaa ggctccacac tctgagctgc catgaggtgc
     3301 ccagccatgt gattaacagg aatcagtgga agtccgtgag cccaagcaaa ggccttggca
     3361 gctgacaaac caactagcaa ggctccgacc aagcctggtc cgtaggtaac cgcaacagct
     3421 gtcacgtcct cttcggtaat ccctgcttct gccaatgcct cctcgataca ggctgtaatg
     3481 acctcgacat ggtgacgact ggctacttcg ggcactacgc caccaaaacg tttgtgactc
     3541 tcaatttgac tagcaatgac attggacaag agctcatcgt cgtttttcaa gacggcgaca
     3601 ctggtctcat cacaggatgt ctcaaatgct aaaatatatc tatccttcat ctatttctct
     3661 cttcatgata atggcgtcct cgactgggtc atggtagtag gcctttcgct cagcgatgac
     3721 cgccattttt tctttcttgt aaaatgcttg cgctcgttga tttgactttc tgacttcgag
     3781 gaaaatctcc ttatctgtcg gcaattgagc aaacaaggct gacgcaattc cctgaccctg
     3841 ataagctcct ttgacagcga tttgcaggac ttctgcttca aaaagattct cctgcacagc
     3901 tagaaatcca atcacttctg ccccatcata agccaatgca taccaagtct ggtcttggga
     3961 cagatctgct tggatttgct ccagagtcca aggactgact agataaacag ctgccataac
     4021 agcgtagatg gcttgagcta ggtcaggctg ttgttgaatt cgcttgattt ctatcatagg
     4081 cgtttaatgt aggactcacc agactcggta tggttcttga gccagttttc ctcagcctcg
     4141 actcgtttga ggtaatttgg cacaaaatca tgcaaggagt ctgcttcctt gtcccaagct
     4201 aaaagagcaa gattggctgc attaggcaag gtttctttat aattagtcct tggcaagtgt
     4261 tcttgaatct gctccacaaa gggggcaact tctccgacaa aggttacctg actagcaccc
     4321 ttgacttttt ctagcacctc ttcaaaagat aggtgcgctt ctggcatgac aggtttagca
     4381 ttttcataaa atcctgcata aacattgttg cgacgcgcat ccatcaaagg aacgaacaag
     4441 ccttcttgtt gatatggcac cagagccaag aggctagaca taccaactaa ctcaatgttc
     4501 agagtatgag tcaaggtctt ggcagtggct accgcgattc gcaagcctgt gtagctacct
     4561 ggcccttccg ccaccacgat tcggtccaaa tccttgggcg tccagtccaa actctccatc
     4621 aaaaaatcaa tggctggcat gagggtaata ctgtgatttt tcttgatatt aatcgtcgtc
     4681 tcagcaagaa cctgcttgtc ctctaaaata gccagagaaa gagccttgct ggacgtatca
     4741 aaagctaata ctttcataac acattcctat ctttttgtct gcttactatt atactacaaa
     4801 agctggcaca tgggaatttt ctttgccccc agacaagagt gccctcactt aactaaaaat
     4861 aatttaaaaa aatgctcact tttccttttc ttttccgaat ataaaagtga acaagaaaaa
     4921 aggaggaaag ttcaatgaca aattttgaca ttcttgacaa tcaattttta tccttatctg
     4981 aaaatgaatt atcagatatt gatggcggtc tcgctccctt ggttatcttt ggagtagcag
     5041 tatcttggaa ggctattgca ggtggaacag cacttatagg ttctggtttg gcagctggtt
     5101 attttttagg aggagattaa tatgatgaaa gatttgaaca actatcgtga aatttctaat
     5161 aaggaattgc aagaaatcaa gggtggcttt ggtgtaggtg ttggtatcgc tttatttatg
     5221 gcaggttata ccattggaaa agaccttcgt aaaaagtttg gtaaatcatg ctagataaga
     5281 aacacatttt tagaaggata aattttattg ccttcatctc ttacagtttg ctcagcattc
     5341 tcaatgattt gaacattact accatccctt taccattcga tttatctgtt tgtattgttt
     5401 tatttttatg cttcaactct atttttgagc agaacaatga ctcccataaa aataataagc
     5461 tttgaaaatt ccattgtcat gtcatgttag aaaaatgcaa agaccacctc atcttgatag
     5521 atggggtgga attttcgtgt cgtaaatcta ctatctctac attcccaaac aaaaaaaacc
     5581 agcataagca gggcatctaa gcatttaatc caaagtaaaa tacaaaccaa acgacatagg
     5641 tcacgaggag gagaaaaagc gagtagagag tcacaaaggt aattttccac aagaacttgg
     5701 tttgtcatcg ttccagtttg gcaaatggaa gatttcctcc ataaacgcaa gcaacaaaaa
     5761 cgataaaagc taacaaacga actccgatag caaaagcaaa taagttatac atagggcaac
     5821 ctccttgact taaaatctat atggaattat gacaagcaat aaatttcact tccgttatca
     5881 acataataca ttttctttat tttcgcaaac gcttaccaaa gaaatcgtcc cctaactttc
     5941 tcgtttccgt cttttactaa tttttcattt tgtggtataa ttgaaataat tgtaacgaat
     6001 caaggtcaat ctagacacaa aatggaatga aatcaagcaa atatctgcta aaagtttgga
     6061 ataagctgac ctgtaaatag aaaggaacta tatgatttac aaagtttttt atcaagaaac
     6121 aaaagaacgt agcccacgcc gtgaaacaac acgcgcactt tacctagaca tcgatgccag
     6181 ctcagaactt gagggtcgca tcactgctcg ccaacttgtc gaagaaaatc gcccagagta
     6241 caatatcgaa tatatcgaac tcttgtctga caaattgctc gattacgaaa aagaaactgg
     6301 cgccttcgaa atcacggagt tctaatatgg cctatactct taaacctgaa gaagtcggcg
     6361 tttttgccat cggtggtctg ggagaaatcg ggaaaaacac ttacggaatt gaataccaag
     6421 acgagattat catcgtcgat gctgggatta aattcccaga agatgacttg cttggtatcg
     6481 actatgtcat tcctgactac tcttacatcg tggacaatat cgaccgcgtc aaggctgttt
     6541 taatcacaca cggacacgag gaccacattg gtgggattcc gttcctactc aagcaagcaa
     6601 atgtccctat ctacgctgga ccgcttgcct tggctttgat ccgtgggaaa ctcgaagaac
     6661 acggcctctt gcgcaacgcc aaactttacg aaatcaacca caacaccgag ttgaccttta
     6721 aaaatctcaa ggcaactttc tttagaacga ctcactctat tccagagcct ttggggattg
     6781 tcattcatac tcctcaaggg aaaatcgtct gtacgggtga ctttaagttc gactttactc
     6841 cagttggaga acctgcggac ttgcatcgta tggctgcgct tggtgaagaa ggcgtgctct
     6901 gtctcctgtc tgactcgaca aatgcggaag taccaacctt taccaactct gaaaaagtcg
     6961 ttggtcagtc cattatgaag attatccaag gtattgaagg acgtatcatc tttgcatcct
     7021 ttgcctcaaa tatcttccgt ctccagcagg caacagaagc tgctgttaag actggacgca
     7081 agattgcggt ctttggtcgt tctatggaaa aggccattgt caacggaatc gatcttggct
     7141 acatcaaagc tcctaaggga acctttatcg agccaaatga aatcaaagat tatcctgcag
     7201 gagaagttct tatcctctgt acaggtagtc agggtgagcc tatggcagct ctctctcgta
     7261 tcgccaacgg aacccaccgt caagtacaat tacaaccagg tgataccgtt atcttctctt
     7321 ctagtcccat ccctggaaac actactagcg tcaacaagct gattaacatc atttctgaag
     7381 ctggtgtcga agttatccac ggtaaagtga acaatatcca tacatctgga cacggtggtc
     7441 agcaagagca aaaactcatg ctctgcttga ttaagccaaa atacttcatg cctgtccacg
     7501 gtgaataccg catgcaaaaa gtccacgctg gactagcagt ggatactggt gttgagaagg
     7561 acaatatctt tatcatgagc aatggcgatg tgcttgccct tactgctgac tcagctcgta
     7621 tcgcaggtca tttcaacgcc caagatatct atgtcgatgg aaatcgtatc ggtgaaattg
     7681 gcgcagctgt cctcaaagat cgtcgcgatc tatctgaaga cggtgtcgtt ctggcagtcg
     7741 caactgttga cttcaaatcg cagatgattc tgtctggtcc agacatcctc agccgaggct
     7801 ttgtctacat gagagagtct ggcgacttga ttcgccaaag ccagcgtatc ctcttcaatg
     7861 ccattcgtat cgcactgaaa aataaggatg ccagcgtgca atctgtcaat ggtgccattg
     7921 tcaacgctat tcgccccttc ctctatgaaa ataccgaacg tgaaccgatc atcatcccga
     7981 tgatcctcac accagatgaa gaataaagca agaaaacagc cccgtcctcg gagctgtttt
     8041 tctctatgct ttcttttgag attaaaactc atactcaatg aaaatcaaag agcaaactag
     8101 gaagctagcc gtaggttgct caaagcactg ctttgaggtt gtagatagaa ctgacgaagt
     8161 cagtagccat acctacggca aggcgacgtt gacgcggttt gaagagattt tcgaagagta
     8221 tcaataaaaa tcgaaatcag actagaaggc taagcgaaag cataacttga gttagctccc
     8281 atagttcggg aaactatggg aggctggaga tgaatcaaag ccaagctttg aactcattcg
     8341 taagaagccg acgacgtatc attttgattt ttgaagagtt ttagaaatac tacgattttt
     8401 accttccaga tacaccatca aaatagaaat atctgctggg tttactcccg aaatacggct
     8461 ggcttggccg atggtttctg gattgatgag tttgaacttc tgacgggctt cggttgcgat
     8521 agaatcaatg tcatcccagt cgatattggc cggaatgcgt ttttcttcca tgcgtttcat
     8581 cttggcaacc tggtccatgg ctttggaaat atagccttca tacttgattt ctgtttcaat
     8641 caattcgata atcttgtcat ccaagtcttc tgcagctggt ccgatgaagg ccaccacatc
     8701 ttggtaagaa acttctggac ggcgaaggaa ttccttggct gtcactgcat cggtcaaggg
     8761 tttgaagccc atctcctcaa ccttggcatt ggtttccttg actggcttga gtttgatact
     8821 gtctaggcgc ttcatctcat tatcaaattg atttttcttg atttcaaaac gagcccagcg
     8881 ttcatcgtcc acaaggccaa tctcgcgtcc catctcagtc aagcgcatat cagcattgtc
     8941 atgacgaaga atgagacggt attcagcacg actggtcaag agacggtagg gttcaatggt
     9001 tcccttggtc accaagtcgt cgatcatcac cccgatataa ccatcactgc gcttcaaaat
     9061 caattcaggc ttgccttgga ttttcagagc cgcattgata cccgcgataa tcccttggcc
     9121 tgctgcctct tcgtaacctg atgttccatt tgtctgacca gcagtgaaga gacctgagat
     9181 tttcttggtt tccaaagtcg cacgcaactg atgaggcaag accatatcat actcaatagc
     9241 ataacctgtc cgcatcatct ctgcattttc caaacctttg atggaatgca ccaagtcacg
     9301 ctggacatcc tcaggcagac tggttgaaag tccttgcaca tagacttcct cagtattgcg
     9361 cccttctggc tcaaggaaga gttggtgacg ttccttgtcc gcaaagcgca caatcttgtc
     9421 ttcaatcgac ggacagtaac gaggccccac tcccttgacc acacctgtaa acataggcgc
     9481 acggtggagg ttgttttgga taatctcatg actggtacca ttggtatagg tcaaccagca
     9541 tggtacttgg tccttgacat aatcctcatc acgtgaagtg tatgagaagt gattaggtgc
     9601 ttcatctcct ggttggattt ctgtcacatc gtaattgata gaagaagcct tgacacgtgg
     9661 aggggttcct gtcttgaaac gaccgatttc gaggcccagt tccttgagat tgtcagctag
     9721 gttaatagaa gccaagctgt ggttaggacc tgatgagtac ttgaggtctc cgatgataat
     9781 ttccccacgg agagcagtcc ctgtcgtcac aataacagcc ttagcagcat attcttgatg
     9841 ggtggctgta cgcacaccga caaccttgcc atcttccacc aaaatctcat caatcatggt
     9901 ttgacgaagg gtcagatttt cttggttttc aaccgtcttg cgcatctcct tagagtaaag
     9961 ttccttgtca gcctgcgcac gaagggcacg gacagctggc cccttccctg tgtttagcat
    10021 cttcatctgg atgtaagtct tgtcaatggt tttggccatc tcgccaccga gggcatcgac
    10081 ttcacgcacg acaatcccct tggcagaacc accgatagag ggattacaag gcatgaaagc
    10141 cagcatttca atattgatgg tcgcaagcag gaccttacag cccatacggc tagcggccaa
    10201 ggaagcctca accccagcgt gtcccgcacc aattacaata atatcgtatt cttcagtaaa
    10261 atgataagtc atgtttctct cctattcctc aagatgaatg tgtcttagtt ggccttccca
    10321 atctggtagg gccgttttta aaaaggctgg aactagctgg atatcctcaa gcttatccaa
    10381 gtcaatccac tcacagggct gccttttctc atcttcctgc atggtcaacg gggcatcttc
    10441 aagcaaatcc accagataat gaaactcgat attgtgatag gaaacgccgt ccacttcaaa
    10501 acgattttca accacaaaag ctagctgccc agcttgagat ttaacaccca gttcttcctt
    10561 cacttcacgg actaccgcgt cttccgtttt ttcgttaact tgaatcgcac cgccgataat
    10621 gtaatacttg cccttgtctt tggtgactag aagcttgcga ttttgaagaa tcaaagctgt
    10681 cgcccgaaca ccaaaaaccg tattgtctac ttttgtccga aagtcttgtt gagtcattct
    10741 tgtcctttcc cttaaacgac acaaaaacag tcaaaactac aaagaagtgc aggacaaaaa
    10801 agcctgcaac atccatgagc tttgaccatc atttctattg ctgttattat tgtagcaaat
    10861 tgagaaaatt tgtcaatcta aatgtactga caaacttgtc catcgcggta agcattgtca
    10921 atcaaaccgc cacctagaca ctcttcgcca tcgtaaaaga caactgcctg tcctggtgta
    10981 atcgcgcgtt gtggttccgc aaagataact tctgccttgt ctcctttgac atggacggtc
    11041 accttagagt caggctgacg gtaacggaat ttagccgtac attctagcgt aaactcttct
    11101 ggcatttcac gagtaaagtg gacttgactg gcttctaggc tagttgacat gagcgaatca
    11161 tggtagaatc cttgtcctac atagagaata ttcttgctta gatcttttcc gacaacgaac
    11221 caaggggcat tgtcaccgcc gtgttgccca ccgataccga gtccgccacg ctgaccgatt
    11281 gtatagtaca taagacctgc atgctcgccc atatcgcgac catccacagt catcatgcga
    11341 ccaggctgag ctggcaggta gttgctgaga aagtttttaa agttcttttc tccgataaag
    11401 caaatccctg tcgagtcttt cttcttagca gtcgaaaggc ctgcttcttc tgctagtctg
    11461 cgtacttcag gcttttccaa atgtcccaga gggaacatgg ttttttggag ttgttcttgc
    11521 gaaagttggc tgaggaaata ggtctggtcc ttgccattgt ccacgccacg aagcatgtga
    11581 acagtgccat cctcatcacg cgccactcga gcatagtgcc cagtcgctac atagtctgcc
    11641 cccaaggtca tggcatagtc caaaaaggcc ttgaacttga tttccttgtt gcacataacg
    11701 tccggatttg gcgtgcgccc tgcacggtat tccgctagga aatactcaaa aacgcggtcc
    11761 cagtactctt tttcaaaatt gacagagtag tagggaatgc caatctggtc tgccaccgca
    11821 accacatcct tgtaatcttc ggtcgccgta cagacgccgt tttcatctgt gtcatcccag
    11881 ttcttcatga agataccgat cacatcgtag ccctgctcct tgagcaaaag agccgtcacc
    11941 gacgaatcaa caccaccact catccccacg acaacacgtg ttttagagtt atcactcatg
    12001 gtaagtctcc catctattcg tttattcacg attgaaggtc gtgtgtgctt cacgattgaa
    12061 ggtcgcttga gcagtattca ttataacacg cttggttaga gaagacaagg aagagcttaa
    12121 aaattgtttt ttaaaactta tagtcaatat acagattatg tgaatgatgt gagtatacct
    12181 tcttcttatc ttcattcaaa taactataat aaataaaaca acccattttc tctaatctat
    12241 ttttcaagtt agagaaaagg agtcgttttt ttaatgacat ttcctgaaat cataagatag
    12301 gtacctaaag aatacgaaca taaatctatt caatcgaacc ttatctttgt ttcaaaatta
    12361 aaatgtcaaa tgttcttaac atgatttaat ttaatcggct taaacccatt caccattggc
    12421 attgacttta tagccatcta cagttgtgtt gactgcaagg gcacctaaac cattgacata
    12481 gtaccattta tctgatactt tgaaccattg gcttgctttc atagcacctg atgcttcaag
    12541 atagtaccag gtatctccat ctttcaccca acctgttgcc attgaaccgt tagcgttgag
    12601 gtagtaccat gaaccgttga ctttagccca acctgttgcc atagcaccgt tagcgttgag
    12661 gtagtaccat gaaccgttga ctttagccca acctgttgcc atagcaccat tagcgttgag
    12721 gtagtaccat gaaccattga ctttagccca acctgttgcc atagcgccgt tagcgtttag
    12781 gtaataccat gaatcgttgt attggagcca tcctgtagcc atagcaccat tagcgtttag
    12841 gtagtaccat gaaccgttga ctttagccca acctgttgcc atagcgccgt tagcgtttag
    12901 gtaataccat gaaccattgt attggagcca tcctgtagcc atagcaccat tgctgttgag
    12961 gtagtaccat gaaccgttgt tttgtagcca acctgtagcc attgaaccat cagtattgta
    13021 gaagtaccac ataccgtttt cttgtttcca gcccgttttt ggtgcgggag ctggttgctc
    13081 tggttttggt gcaggagctg gagctggttt ttctgctttt ggcggttgct gttgagtcaa
    13141 gcggttatat tcttcttctg atctacgagc atagtcttct tcagcttgtt gatctgctga
    13201 cttctctggt tttggtgcag gagctggagt ttcttcagct ggtttttctg gttttggttg
    13261 ttcagctggt gcttctgggg ctggagtttc ttcttcatct ccatcagggc ctaactcatt
    13321 aagagctgca tctaactctt tttgagtttt ttctaactca gcttgtttag tcgcgatagc
    13381 ttcttctaaa ccttctttaa tgtagtcttc aacgttgttt gtttcagcat ctttaagatt
    13441 atcttcaagt tttgaaagtt cttcttctaa ttcagcaact tggttttgaa gagcttcaac
    13501 ttttttattc aactcagctt cagcagcttc tttatctaat tcatcctgag tcttaccttc
    13561 agggtctagt gtagataata ggttttcaag ttcagcttct gctttttcta attcaggttc
    13621 taattcagct tttttagtag cagctttaat tttagcttct gctaatttag cttttttagt
    13681 agctaggtct ttttcagcag cttcaaggta gaatttggca tattcaccat ctgattcttt
    13741 gaaaccttct acatcttttt caagtttagc aacttctttt tctaaatctg ctaccgtttt
    13801 ttcagcatca gctatttctt tttctaaatc agcaacttta ttttgaagtt cagcaacttt
    13861 tttatccaac tcagcttctt ctgcctctac ttctttcttc gctacttcta cctcttgagc
    13921 tgccttatca tatttctcct tagctacttc ggcgcctttt ttagtttctt ccgctttttg
    13981 tttagtaaca gctaactcct ttgcttcagg aactacaact gctcttacag cataaaattt
    14041 agattgcttt tcatctaata ctgtttttgt atcctttatt ttttgatctg cttcagcgag
    14101 atctttattg tacttatcac gattattttt atattttttt gaatcttttg ctcgttgaat
    14161 ttcaaaatac cttacatatg ctttttgatg cacttctgtg gcttcgttca agtctgctgc
    14221 tgcctttttt tcattttcgg ctttctcttc agttttcttc tgatcctcat catatttttt
    14281 ctgagcgtct tctgctttct ttttagcttc ttcgtaagcc ttcttagcag cttcagattt
    14341 tttcactgct gcatcatagt ctttctcagc tttagactga ctagctacgg gggcttcttc
    14401 tgctcttaca aaagtaggct gagacgtaac aaaaccagcc cctaagatag cgacgctggc
    14461 tagacttgtt aaaatcattt ttttcttatt catctaaatt tacctctttt ctgatagtca
//
LOCUS       DAVLWA010000044        14309 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLWA010000044 DAVLWA010000000
VERSION     DAVLWA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.044"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(10..1299)
                     /locus_tag="VYN80_001274"
     CDS             complement(10..1299)
                     /locus_tag="VYN80_001274"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1541180.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKEA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVSYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFVAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1377..2612)
                     /locus_tag="VYN80_001275"
     CDS             complement(1377..2612)
                     /locus_tag="VYN80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1541181.1"
                     /translation="MKLLFRNLAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVWLLAL
                     SVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFLTIYR
                     NLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQF
                     CVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYV
                     SGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLTWMLF
                     VGLSLLAIFLTILNLKNDI"
     gene            complement(2803..3654)
                     /gene="rgg"
                     /locus_tag="VYN80_001276"
     CDS             complement(2803..3654)
                     /gene="rgg"
                     /locus_tag="VYN80_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1541182.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKGNETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3778..4119
                     /locus_tag="VYN80_001277"
     CDS             3778..4119
                     /locus_tag="VYN80_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1541183.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4177..4257
                     /locus_tag="VYN80_001278"
                     /pseudo
     CDS             4177..4257
                     /locus_tag="VYN80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4418..6121)
                     /gene="ilvD"
                     /locus_tag="VYN80_001279"
     CDS             complement(4418..6121)
                     /gene="ilvD"
                     /locus_tag="VYN80_001279"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1541184.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6354..7286)
                     /locus_tag="VYN80_001280"
     CDS             complement(6354..7286)
                     /locus_tag="VYN80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1541185.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7283..8140)
                     /locus_tag="VYN80_001281"
     CDS             complement(7283..8140)
                     /locus_tag="VYN80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1541186.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8144..9490)
                     /locus_tag="VYN80_001282"
     CDS             complement(8144..9490)
                     /locus_tag="VYN80_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1541187.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFITKPSAKEE"
     gene            complement(9503..9787)
                     /locus_tag="VYN80_001283"
     CDS             complement(9503..9787)
                     /locus_tag="VYN80_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541188.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9791..11821)
                     /locus_tag="VYN80_001284"
     CDS             complement(9791..11821)
                     /locus_tag="VYN80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1541189.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12012..13019
                     /locus_tag="VYN80_001285"
     CDS             12012..13019
                     /locus_tag="VYN80_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1541190.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13022..13222
                     /locus_tag="VYN80_001286"
     CDS             13022..13222
                     /locus_tag="VYN80_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1541191.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13374..13556
                     /gene="rpmF"
                     /locus_tag="VYN80_001287"
     CDS             13374..13556
                     /gene="rpmF"
                     /locus_tag="VYN80_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1541192.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13572..13721
                     /gene="rpmG"
                     /locus_tag="VYN80_001288"
     CDS             13572..13721
                     /gene="rpmG"
                     /locus_tag="VYN80_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1541193.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14200..>14309)
                     /locus_tag="VYN80_001289"
     CDS             complement(14200..>14309)
                     /locus_tag="VYN80_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1541194.1"
                     /translation="TVSGQWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         4624 a         3015 c         2463 g         4207 t
ORIGIN      
        1 ctttgatttt tattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct
       61 gattgtctca agtgacactt gcacttcttc tcgagtttgg ttgttcttga ccgtcacttg
      121 tccgctttcg acttcgctct ctcctagggt gatgagggtc ttagccacaa agacatcggc
      181 tgacttgaac tgagctttta gtttacggtt gaggtaatca cgctctgctt tgaaaccttg
      241 ttggcgaaga gcctgtacca attccaaggc cttgatattt gccccttcgc ccaagactgc
      301 gatatagaca tctagggcgt tttcgatagg gagggtcaca ccttgctttt caagaatgag
      361 aagcaggcgc tctacaccaa gtccaaaacc aaatccagca gtttcagggc ctccaaaata
      421 agaaaccaaa ccatcgtaac gaccacccgc acagacggtc aggtcattgc cctcaatctc
      481 tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc
      541 gatgatgtaa tctactccaa gattttccaa catctgacgc acagcatcaa aatgagcttg
      601 gctttcttca tcaagaaagt ccaagataga cggcgcattc tctactgcct ccttgtcttc
      661 tttttcctta gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt
      721 agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg
      781 gctctcagga tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt
      841 caaaaaatgg gctgccatag cgattgtttc cacatcggta gctggattgc tagagccaaa
      901 acactcaaca ccaatttggt ggaattggcg caagcgccct gcctgtggac gctcataacg
      961 gaacataggt cccatatagt agaacttgct tggcttttgc acttctgggg cgaagagttt
     1021 attttccaca taggaacgga caacgggcgc agttccttct ggacggaggg taatatgacg
     1081 gtcacccttg tcataaaaat cgtacatttc cttggttacg atatccgttg tatctccgac
     1141 agagcgactg ataacctcgt aatgctcaaa aataggcgtg cgcacttctg catagttata
     1201 gcgcttgaaa atctcacggg caaagccctc aacgtactgc cacttagcag attcagcagg
     1261 taaaatatcc tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta
     1321 atagtcttat tttaccacaa ataaagggat taaaacagta agaaaaaaat taggatttag
     1381 atatcatttt tgagattaag aattgtcaaa aaaatagcta gcaaggaaag accaacaaat
     1441 agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca
     1501 ggtatggata aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga
     1561 gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacagg
     1621 gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact
     1681 agactaagtc ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa
     1741 tcattgcccg taaggctact gatgatgatt gctactaaaa cacagaattg attgataaat
     1801 agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt
     1861 ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag
     1921 tttttctgcc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg
     1981 tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg
     2041 ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg
     2101 attcccgcaa cgagagaaaa attgttgacc gacagagcta agagccagac tccgaaagct
     2161 tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga
     2221 tcatcttcag ctactttttc cttaataaga ggcatgcgca agccacctgc aaaatcactg
     2281 atgacattga tcaaacacag gctagaaaag gcaaagagac tagcttgctg aacaactagg
     2341 gctgctagaa aaaatagaac cgcctgaaac aaaccgctat agaccatcca tttgaccttg
     2401 tccctcgtgt aatctgcccg aatccctaca aaaactgtaa agagggtcgg aagaatcatg
     2461 acaatattcg ccatagcaac agcaaaagat gcttgtgaca aggtcgatgc atagacgata
     2521 aagaccaggt tgaaaatcga agcaccaaaa gcattgaaga agcgtgataa agtcaaaatt
     2581 ctataagcta gatttctaaa caatagtttc atagataatc tcccttcttg ttctaagttc
     2641 tacatcaaga atagctttag tatagcactt atgaatttgc gaaacgattc ttttgaatat
     2701 atgcaacaaa taaaatgagc aaaaaaacga tagaactttt gtaatgacct gagagagggt
     2761 aacagttcta tcgcttcaaa ttttttcttg gtttgcagat actcaacatt cggtcgtatt
     2821 tctatagtat tcggcagatt tattacagcc aagcatctca aaaatacgga cagcatcctc
     2881 catctttttc tggccttcct tgactctacc ttgcttgcta tcaaggagac cttctgccca
     2941 cagataaaca attcggaaat aggtctcatt tcccttgtag aaatgctctt cgataacacg
     3001 tttaaaataa taggcattgg taaattcttc acactcaata ctagctaaaa agccattcaa
     3061 tagtatagta tgaaaaaggt ttcgattgcc agacatttcc attagaaaat cggatttacg
     3121 taccatttct cgtacatatc tagtaaaaag agaaacagat aaaaatggag aactgactga
     3181 aaataaattg agttcataga ttccccagat ctcggtagaa aacaaataat catgaaggac
     3241 ttttccttcc tctgctgtta agtctaccct ttcatctatg ctcttcatat aagacttgat
     3301 aataatggca tttagaatat gtttctgttt gttgtgagaa tgggcatgct tttgatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctc gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgattctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat catcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag caggccaatc acttttaaag
     4141 atgaaaaaag caagccaaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgctcaaaa cactgttttg aagttatgga tagaactgac
     4261 gaagtcagtt caaaatactg ttttgaagtt atggatagaa ctgacgaagt cagctcaaaa
     4321 tactgttttg aggttgtgga tagaactgac gaagtcagta accataccta cggcaaggcg
     4381 acgttgacgt gatttgaaga gattttcgag tatgagttta ttttttacct gacttgtcca
     4441 tattccagaa gtctgtcacg gctccgcgtg aagcagatga tacgatgtgg gcatatttac
     4501 cgaggacacc acggctgtaa agtggtggca aggttgtttc tgccttgcgt ttttcaagtt
     4561 cttcttcgga tacggccatg gaaatttctt tggtatcttg gtcaaccgta acgatatcgc
     4621 cggtacggag ataggcaatt ggtccaccat cctgagcttc aggagcgata tgtccaacaa
     4681 ccagaccata agtaccacca gagaaacgtc cgtccgtcaa gagggccacc ttatctccct
     4741 gacctttacc aacaatcatt gaagaaagtg acagcatctc aggcatacca ggaccacctt
     4801 taggtccaac aaaacgaaca acgactacat cgccatcaac gatttcatct gtcagaacgg
     4861 cctgaatcgc atcttcttct gagtcaaaga ccttagctgg cccaacgtga cgacgcactt
     4921 taacacctga taccttggca actgcaccgt caggagcaag gttcccgttc aagatgataa
     4981 gcggaccatc cgcacgtttt ggattttcaa gtggcatgat aactttttgg cctggagtca
     5041 agtctgcaaa gtcagccaag ttttcagcta cagtcttacc agtacatgtg atgcgatctc
     5101 cgtgaaggaa accatttgcc aacaagtact tcataaccgc agggacacca ccgacttcgt
     5161 agaggtcttg gaagacatac tgaccagatg gtttcaagtc ggccaagtga ggcacacgtt
     5221 cttgaatcgt attgaagtcc tcaagtgaca agtcaacatt tgcggcatgg gcaatggcga
     5281 gcaagtgaag agtggcgttt gtagaaccac cgagagccat cgttacagta atagcatctt
     5341 caaaggcttc acgagtcaag atatctgatg gtttgagacc aagttccaac atcttaacaa
     5401 cagcacgtcc tgctgcttcg atatcttctt tcttatcagc tgattcagct gggtgagagg
     5461 atgaccctgg caaactcatc cctagaactt cgatagcagt tgccatggta ttagcagtat
     5521 acataccacc acagccacca gggccagggc aggcattaca ttcaagacgt ttcacgtcct
     5581 cagctgtcat gtcaccgtgg ttccattttc cgataccttc aaagacagaa accaagtcga
     5641 tatctttacc atcaagattt cccggtgcaa tagttccacc ataggcgaaa atagctggga
     5701 tatccatatt agcaatagca atcatagatc caggcatgtt cttgtcacag ccaccgatag
     5761 cgacaaaggc atccacgttg tgaccactca tagccgcctc gatggagtcc gcgatgatgt
     5821 cacgagatgt tagagagaaa cgcataccag gcgttcccat agcgatcccg tctgctacgg
     5881 taatggttcc aaactgtaca ggccaagcgc ctgcagattt gacaccttct ttagccagtt
     5941 tcccgaaatc atgcaagtga atgttacatg gtgtattttc cgcccaagtc gaaatcactc
     6001 ccacaatcgg tgtttcaaag tccttatctg tcataccagt cgcacgaagc atagcacggt
     6061 taggtgattt aaccatgctg tcataaatgc tactgcggtg acgtttatct aattcagtca
     6121 tcttatccct cccatttcag tttttactat tatagcacaa ttttcgcatg aagaacagaa
     6181 taaaaatctt gaattttcag aaaattctat acacatgtga aatatttaaa attaaaaaca
     6241 acaaagcgga ttagtgcact ttctgatgac cagaatatgc tttttaatcc gctttcttta
     6301 aataacgtac tgtaattttt acagaaattc tttcagataa gtgtatttaa catctatctt
     6361 gcattataaa tttctagaac cttctctttt atattcgatt cactcaaacc atactcatta
     6421 agaagataat ccattttccc tacttgaccg aatctttctt gaacacccat ccgatgaatt
     6481 tttgtcattc catcatcaga gaataattca cataaagaac tgccaattcc acctatctga
     6541 ttgtggtttt ctacagtaaa tatagttttt ccacttaaca ttgtttttat ctgttctggt
     6601 atcggtttga ttctaaataa atctatcaca cttactgaat aacctaattt agacagttca
     6661 tctgcaactc gaatacttgg agcaaccatt ataccagaag caacgattac aagatcttca
     6721 ccatgcctta actcaatgta gcctttagaa aaatcttctc caccttgata cacaggaact
     6781 ggagcttttc taattgttcg aatatatttt agtcctttta agtctaatgt ctggttcaat
     6841 atttcacgaa attggatatc atcagttgct tcaaaaatga tcgatttagg aattaaacgt
     6901 aacaatccaa tttcttcaaa tggcatatgt gtcccaccat tcatttctgc agttactcct
     6961 gcatctgatc caatcacagt ggcatccaat tgtgcgtatc caagagaaat aaataattga
     7021 tcaaatactc ttcgtgaagc aaaaggacca aatgtatgaa gataaggtct aaacccctga
     7081 atagacaagc ctgctgcaag gccgaccatt tctgcttcca taatcccaac attcacataa
     7141 cggtctccaa aatccttttc aagattatta gtagccatcg aacttgacaa atcggcttct
     7201 aagactacta tatcagaata actttgatta gcctctaaaa ggaagtctct atatacatgc
     7261 cgtaattctt tcgtgcttct catcattctg tttcctccaa ttcctgactt aatctttcta
     7321 caactgaagt taacatttgt ctctcctcta cagtagggcg aagatgatgg ttggatttca
     7381 tttcttccag ctcttgaacc ccttgacctt taatagtatc taatacaatg cacttaggtg
     7441 atgaattatt tgactgtttt aattggacaa tcccttcata aatttctcta atatctgaac
     7501 ccttgaccct aatggattca aatccaaatg ctgaaaattt ttctacgaaa tcacctggat
     7561 tacaaatatc ctttgtaaaa ccatctaatt gttttttgtt atcatcaaca aatacaatta
     7621 agttggataa ctgttgatga gaagcaaact gtatagcctc ccaacattgt ccctcattta
     7681 actcaccatc tccaacaata gcataagtat aaaagggact ctttcttatt ctctgaccat
     7741 atgcaagtcc agttgcaaca ctaattcctt gtcctaaaga gcccgttgtc atatctatgc
     7801 ctggcgttag atttctatca ggatgagacg gtaatttggt tccatttgta tttaaagaat
     7861 ataagaattc tttgtcaaag aaaccattca aatagagtgt actgtataga gctggtcctc
     7921 cgtgaccttt tgataatatg aaataatctc tatctcgtgc tgcaaatatt tctggagtca
     7981 ttggcattat ttcaccataa agcactgcta aaacttctac gatagacaga cttcctccgt
     8041 aatgtccgaa tccaagatga ttcaatgttc taagagtatt taatcggatg ttagtcgcaa
     8101 attttcttaa ctcatcttcc ctatttttac ttaaaatcat cccttattcc tcctttgcag
     8161 atggctttgt aataaaggat actccaaaca taactgctag aataagaaca agaccaatca
     8221 caatgcctgc ttgtgagcca aattgattta acattcctaa aataattcct gatagaccaa
     8281 aatctgcatc tgagaaagtt gatccttgga aaccaagtcc tcccaaaact ggcattaaaa
     8341 agactggaag aaaactgatt aaaatacctt gtaaaaatgc tccaatagtg gctccacgaa
     8401 caccaccaga tgcattccca atgacacctg cagtcgctcc acagaagaaa tgaggcacaa
     8461 cacctggtaa gataacaacc gttcctgaag caatcataat tgccatactt actaaaccac
     8521 caacaaaact agagataaat ccaattagaa ctgcattggg tgcataagta taaacaatcg
     8581 gacaatccaa agcaggtttt gaattaggta caagacgctc tgaaatacct ttaaaggctg
     8641 gaacaatttc gcccaaaata aggcgaacac ctgctaaaat aacaaatacc cctgctgcaa
     8701 attgacctgc taattgtaaa gcataaacta gaccacttgt accactactg atttcttttt
     8761 ctatatattc tgaccctgca aagatagcta caataatgta aataactgcc atggataaag
     8821 tgatactaac agtactatca cgtaaaaaag ctaaactctt tggaaattta atgtcctctg
     8881 ttgattttga tttgtcaccg ataaggctac cagtaaaacc actcaaccaa tatcccaaag
     8941 aactgaaatg acctaaagcc accttgtcat ttccagttaa ttgaaccata tatttttgaa
     9001 caaatgctgg ggaaatactc ataataatac cgagtgctaa tcctcctagt aagataagag
     9061 gcaagctagt aaagccagca actgataaaa tgaccgcaat catacatgcc atatatagag
     9121 tgtggtgccc tgttaaaaaa atatatttaa atcgagtaaa acgagcgatt aagatattga
     9181 acaccatgcc tgcaaacata atcagtgcag tagctgagcc atatgttgtt aaagctacag
     9241 ctacaattgc ttcattattc ggcacaacgc cagataaatg aaaagcatgc tcaaacatgg
     9301 taccaaatgg atttaaagaa ttttgtacaa ttcctgcacc accagataca actaagaaac
     9361 caacaaaggt cttaattcca cctttaataa tatcaggtaa tttcttcttc tgaagaacta
     9421 atcctaaaat tgcaattaaa gctactaaaa tagctggtgt actaacaata tccaatatga
     9481 acttcatcat aacgctagcc tcctatataa gtcctttttc ttcacaaagt ttagtaatta
     9541 attctcgtag ttcatccata tcaataatac tatttaagat acgaacatct ccaagatgac
     9601 tagctgaatc agctagatca cgaccaacaa tccaaatatc agctgcattt ggatctgctc
     9661 cacctaaatc ataatgttca acttctacat ccgaaacatt caaatcactc aatacagatt
     9721 caatattcat ctgtaccata aaacttgaac ctaatcctga accacaagct gtaccaattt
     9781 ttaacattat ctaatcctcc tgtttaatta tcattttaat gtcatcatag ttttttgatg
     9841 atattaaagt ttgaacatga tttttatctc ttaaaattgt tgttaaatgt gacaaagcct
     9901 ttaaatgact ctcattatca atggctgcaa tacaaatcaa caatcttacc tcttgttctg
     9961 gattatccaa taaataaatc ggttcttcca aaactaacat tgacattcct atttcattca
    10021 caccttcatc tggccgagcg tgaggaattg ctactccctt ccctaaatta ataaaaggcc
    10081 caaactcttc tactttttga atcattgcct cagggtagtt ctcagttatc ttatcttgat
    10141 ccaaaagcgg tttagctgct aaacgaatcg cctccttcca tcctaatttt tgcgaactaa
    10201 cctgataggt ttctttggta atgagttgtt ctagcactgg tacaatttcc tttctatcat
    10261 ttttttggta aagataattc tttaacgcca atcttaattc caattcttgt gtaataattc
    10321 catatctttt gacaatattc aggatttgtt caatctcaaa atccccatac tctaaattcg
    10381 gaaaatcttt taacactagt tctactagtt gtattgcttg ctcttcagtc atcataaccg
    10441 aaactagata atttggcttt tctgtctcca cctttatggt agaaaaaacc atatcatagt
    10501 cactactagc tttcacctgt aaatcatcaa tctttgaggt tcctataaac tcaatttgag
    10561 gaaataatgc taatagattc tcttttaaca tcaatgaaga actaacacca ttaggacaaa
    10621 tgattgctgc tttatagcat ttttgaggca aagtatctgc tttctttaaa taacctccga
    10681 aatggataac aaaatatgct gtttcactat caggtatggg attgtcaata gcgtccatca
    10741 agggcatcaa agaatctttg actaattcaa ataaatcagg ataatgttct ttaacatgca
    10801 atacatattc atttgaacta ggtaggccga actttaatct atagtaagcc ggtataaggt
    10861 ggcggcgaag attttctctc aatccttccc tttgtttaaa atgtaacaaa gaaatatctt
    10921 ccattctact tataatagcc tctgttaatt gattaaagta aaccggagca acatctactt
    10981 caccttcaaa gcaacttgat aataaaacgg tgatatagcg ataatcatcc tcagaaaaca
    11041 ccgtatctat aattcccaaa tcaaccactg tatccaataa aatagtcgtt atatcttgaa
    11101 taacaggaga tactaatgtc tctgaaagac atactctttc aacatccctt tgatacctac
    11161 acagaatgaa tactaaaccg aaaaggcaaa cttttaattg attaacaata ggtactagct
    11221 gtagcttctc ataataatct ttaactacct gatttatcaa atcataagtt aatgaatacc
    11281 cccaactgga taaaacataa tccaaacccc aaatccctat ggaggattcc agcaactcac
    11341 taaccatttg aaaagctaag cggtgcttat tccactctga accgtgtaaa gtataacctt
    11401 ttgctctact gtaccctagc tccaaatcat tatctaacat aatctttctt aatgattgaa
    11461 tatcagataa ggttgtattc ttacttactt tcaaaaagtc ttggtaatga ttattcgata
    11521 taaaatctaa tcggcaaaaa gtgtaaagat agattaaagc taagcgagtc gactttggta
    11581 aaaccaattc atccgactta ataatatctg tcaaagactg cttcgtacga tttgataaac
    11641 tatagcgacc ttgtttttta tccagcacta tccctttatt agctagataa ggcactaaat
    11701 aatctattcc ttctttgact tcctttatag gtaagctcac cttaacagat aattcatata
    11761 atgatagctc acaatgatcc atcaaagtca tcaaaataac tagtgctcta taatcaaaca
    11821 tttctcttcc tttctaacta caattatagt gtaaaaatta taagaataaa agagtatttg
    11881 agcacaatat aatctcacct tttaaaagaa attattgggc tgaaactttt tagtgattat
    11941 tttcaacttt ctattgactt ttaaaaacaa agtgatatca tttagatatc aaataatagg
    12001 aggtcattct tatgactgaa aaactcatca attcaaaacc aaatggtgta ttcgcattga
    12061 ttctcattga attgacaatc gtacttggta tctttatatt tataatgggt gttggttcgg
    12121 aaaacatttt tggaattatt atcggacctt tactaatcgt aattgcaggt ctagctcatg
    12181 ctggtttaaa agttgtcaaa cctcaggaag ctctggttct gacactcttt ggtaattata
    12241 caggtaccat caaagaacct ggtttttact ttgtcaatcc cttcagcgta gcagtcaacc
    12301 ctgcaaacca cactcgactt ggacaaagtg gtgatgttag cacaaaatct ccttttttag
    12361 gagctaaatc atcaaatgac aatgatgtaa atcttgaaat tggcaagaaa cagatttccc
    12421 tcaaagtcat gaccttgagc aattctcgtc aaaaaatcaa tgattgctta ggaaaccctg
    12481 tagaaatcgg tatcgcggta acttggagag ttgtcgatac cgctaaggca gtcttcaacg
    12541 ttgataacta caaagaatat ctttcattgc aatgtgatag cgccctccgt aatattgtcc
    12601 gcatctatcc ttacgatgtg tctcctaatg tggatactac gggtgacggg caagcagatg
    12661 aaggtagtct ccgtggctct agcgaaattg ttgctaaccg tattcgtgaa gaaatccaaa
    12721 gtcgtgttga ggatgctggc ttggaaatcc ttgaagcacg tatcacttac ctagcttatg
    12781 ctccagaaat tgctgccgtt atgcttcaac gccaacaagc atctgccatt attgatgcac
    12841 gtaagatgat tgtagatggt gctgtaggaa tggttgaaat ggcactagaa cggctcaatg
    12901 aaggggaatt ggtagaactt gacgaagaac gaaaagctgc catggtttca aatctccttg
    12961 tcgttctttg tggcaatcat gatgcacaac caattgtcaa cacaggaagt ctttactaaa
    13021 gatggctgaa gctaaaaaaa agcagatccc ccttcgactc tcaacaaagt tatacgctgc
    13081 actcgcatca tgggcagaag acgactttcg ttcagtcaat gggcaaatag aatatctcct
    13141 cacagagtgt gtcaagcaac ggaaaaaaga cggaaaatac gtatcagaaa ccattgacga
    13201 accatttgag attgatattt aacaccttct cctgtctgcc gtgtcggata ggagaatttt
    13261 ttcatctttt tttgtcaagt aactttactt tacaaaaaaa atgtgttatc ctagtatggt
    13321 tgatgaaaat cagtagattg aatcgaatat aaatacctaa aggagaaatc aaaatggcag
    13381 tacctgcacg tcgcacttca aaagcgaaga aaaacaaacg tcgtacacac tacaaagtaa
    13441 cagctccatc tgtaaacttt gacgaaacta ctggagatta ctcacgttct caccgtgtat
    13501 cacttaaagg atactacaaa ggacgtaaaa tcgctaaagc tgcatcagct gaataataga
    13561 agggagatac tatgcgcgta aatattacac ttgaacacaa agaatctggt gaacgcttgt
    13621 accttacttc taaaaacaaa cgtaacactc cagaccgcct tcaattgaag aaatactcac
    13681 caaaacttcg caaacacgtt gtgtttacag aagtgaagta agcattcaat acgaatcaat
    13741 acagaagaaa aacgcttgat cagcgttttt tctttttgtt aatttcacta tattgcaagg
    13801 gaaatcattc gaatgactac attattgaaa taagaatacg gattcggaga attaaaagca
    13861 agaggaactc aaatgtaagt tggggttcct tttttgtata tttttcaaat aacgcctcta
    13921 cactatttgt agcaaattca ccaactacag ttgtatctta gttaaaataa gttagaatat
    13981 gtaagtgagt accagatata ccaagacatc gtcaccatct aaggtatatt caaaatacaa
    14041 aagttgacca actagatttc tgaatatcct tatatatcca ttcttaaaat tggtttaaat
    14101 agcgtagtct tttaaactag ttttgagaat ccaaaaaatc ttcctacata tgtaagaaga
    14161 ttttttagtt cagaatgatt agatttagct aatggatacc tatcctaccc actcaccgtt
    14221 agcattgact cgatagccat cgggtgtagt cgtgtttact aataaatctc ctgaattata
    14281 ggtatagtac cattgaccag aaactgtaa
//
LOCUS       DAVLWA010000045        14201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLWA010000045 DAVLWA010000000
VERSION     DAVLWA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.045"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            71..868
                     /locus_tag="VYN80_001290"
     CDS             71..868
                     /locus_tag="VYN80_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1541195.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1016..2275
                     /locus_tag="VYN80_001291"
                     /pseudo
     CDS             1016..2275
                     /locus_tag="VYN80_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2371..3645
                     /locus_tag="VYN80_001292"
     CDS             2371..3645
                     /locus_tag="VYN80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1541196.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEHYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3655..4533
                     /gene="truB"
                     /locus_tag="VYN80_001293"
     CDS             3655..4533
                     /gene="truB"
                     /locus_tag="VYN80_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1541197.1"
                     /translation="MNGIINLKKEAGITSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4794..5096
                     /locus_tag="VYN80_001294"
     CDS             4794..5096
                     /locus_tag="VYN80_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1541198.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5204..5842
                     /gene="udk"
                     /locus_tag="VYN80_001295"
     CDS             5204..5842
                     /gene="udk"
                     /locus_tag="VYN80_001295"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1541199.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLEGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKISKILEEARNSK"
     gene            5970..7310
                     /locus_tag="VYN80_001296"
     CDS             5970..7310
                     /locus_tag="VYN80_001296"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1541200.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERLKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEEAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7288..7500
                     /locus_tag="VYN80_001297"
     CDS             7288..7500
                     /locus_tag="VYN80_001297"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1541201.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7497..8372
                     /locus_tag="VYN80_001298"
     CDS             7497..8372
                     /locus_tag="VYN80_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1541202.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8365..9180
                     /locus_tag="VYN80_001299"
     CDS             8365..9180
                     /locus_tag="VYN80_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1541203.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9173..9604
                     /locus_tag="VYN80_001300"
     CDS             9173..9604
                     /locus_tag="VYN80_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1541204.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9611..11278
                     /gene="recN"
                     /locus_tag="VYN80_001301"
     CDS             9611..11278
                     /gene="recN"
                     /locus_tag="VYN80_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1541205.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11280..12008
                     /locus_tag="VYN80_001302"
     CDS             11280..12008
                     /locus_tag="VYN80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1541206.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12087..13910
                     /gene="lepA"
                     /locus_tag="VYN80_001303"
     CDS             12087..13910
                     /gene="lepA"
                     /locus_tag="VYN80_001303"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1541207.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13991..>14201)
                     /locus_tag="VYN80_001304"
     CDS             complement(13991..>14201)
                     /locus_tag="VYN80_001304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541208.1"
                     /translation="AGYSKHSFEVVDETDEVSSKHGFEVVDETDEVSSKHGFEIVDET
                     DEVSNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         4199 a         2596 c         3368 g         4038 t
ORIGIN      
        1 tatagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa aaaagattta
       61 ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt gcaataagaa
      121 agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt tcgcaggtgc
      181 atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta ataaaattgc
      241 accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg cctacattgt
      301 ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg gtagtttctt
      361 aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct tgttcaacct
      421 tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg cgaatctgac
      481 acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa atgaattggt
      541 cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt catttatttt
      601 ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca tgtttgtatt
      661 cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga aattcagtgt
      721 tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact ggggtgtgac
      781 tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg ccttcctcaa
      841 taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg tgcttttttc
      901 attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact gatatttgta
      961 aactataact caaggtgcta caatatcctt aataaaataa tatggaggtc accttatgac
     1021 tcgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg ctccagctac
     1081 taagtctcgt gcagtgccga tttatcaaac aacatttttt gtttttgatg acacgtagga
     1141 aggtgccgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta tcaccaatcc
     1201 tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag gtatgactgc
     1261 agtgacttat acgattttgg cgcttgccca tgctggtgag catgtagtgg ctgcttcgac
     1321 tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg gtatcacaac
     1381 aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag acaataccaa
     1441 gcttgtcttg attgaaacct tgggtaaccc cttgattaat attccagacc tggaaaaact
     1501 ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt ttgcaacacc
     1561 ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg cgactaagtt
     1621 tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc gttttgactg
     1681 gacggcttca gggaaattcc ctcaatttgt tgacgagagt ccaagctgcc acaatttgag
     1741 ctatactcat gatgtgggtg cagcagcctt tattatagct gttcgagttc aattgcttcg
     1801 tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa gacttgaaac
     1861 cctttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg attttcttgt
     1921 caaccatctt aaggtagaaa aggtaaatta tccaaaactt gcagatagtc cttattatgc
     1981 cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc acgtcaaagg
     2041 tgacgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg accttgcaaa
     2101 cgcggcagat gttaaatcgc ttgttgtcca tccagcaaca atcactcacg gtcaattgtc
     2161 agaaaaagac ctagaagcag caggtgtcac accaaatcaa atccacttgt ctatcggtct
     2221 tgaaaatgta gaagatttga ttgaagactt acgcttggcc ttggaaaaaa tttaaagtaa
     2281 aagaagataa acagtgggtt ttgactcact gtttttgatt ttccctcagg catgatataa
     2341 tggttacaga agtctagaaa gaggaatgta atgaacgaaa tcaaatgtcc caactgtggg
     2401 gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt gagaacggca
     2461 gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc tgagcaaaag
     2521 gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc gcaattgcag
     2581 agtcaaatcc aaaactttga tacagaaaaa gaattggcca agaaagaagt tgaacagaca
     2641 agccatcagg ccttattggc taaggacaag gaagtacagg ccttggaaaa ccagttggcg
     2701 accttgcgtt tggagcatga aaatcaattg caaaagaccc tttctgacct agaaaaagaa
     2761 cgcaatcagg tcaaaaatca gctcctactg caagaaaagg aaaatgagtt gtctttggct
     2821 tctgttaagc aaaactacga agcccagctc aaagcagcca gtgaacaagt cgagttttat
     2881 aagaatttca aagctcaaca atccacaaaa gccatcgggg aaagtttgga acactatgcg
     2941 gaaagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt tgagaaggat
     3001 aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg tgatgaaaat
     3061 ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg aacagagaag
     3121 aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga gaagaactgt
     3181 gagtatgccg ttttggtgac catgcttgag gctgataatg actactttaa cacagggatt
     3241 gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt ctttatccaa
     3301 ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca ggagttggcc
     3361 ttggttcgcg agcaaaatat tgacattacg cattttgagg aagatttgga tgcctttaag
     3421 ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc tattgatgaa
     3481 atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac atctgaaaac
     3541 caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt gacccggaaa
     3601 aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac
     3661 ggtattatca acttaaaaaa agaagcagga ataacctcgc atgatgcggt ttttaaactg
     3721 cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc ggatgtggtg
     3781 ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat gcaggacgag
     3841 ggtaagatct atgaggggga aattactctg ggttattcca cgacgactga ggatgctagt
     3901 ggggaagtaa tcgcagaaac ccctgttttg tctcccttgg atgaaaagct tgttgatgaa
     3961 gcgattgcta gcttgactgg gcctattacc cagattcccc ctatgtattc ggcagttaag
     4021 gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa
     4081 cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga tggccaactt
     4141 gctcgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac tttgtcagtt
     4201 gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg tactagtgct
     4261 gctggcttac aattagaaga tgctcttgcc ttggaggaaa ttgctgaaaa agtagaggct
     4321 gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt caaagttttc
     4381 ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct agaccaaacg
     4441 gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga aaaacggggc
     4501 aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa atcgggtgat
     4561 agataataat tgcttgataa aaccccatac taatagtaga atggttttgg gaattataat
     4621 attccaattg ttgcgagttg taggtactca aataatctat taggtactca aataatctat
     4681 atagaaattt agaggtgtga aatgaagcaa tttaaaattc tttcagataa atatttagag
     4741 tccattacag gttctggtgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc
     4801 gaaatgagtt tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata
     4861 gtgagttgag tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc
     4921 aatcggtcat ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc
     4981 aggcacctaa gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa
     5041 gagggagact ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact
     5101 gtcgaacatc agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa
     5161 aaatgtgata gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat
     5221 cattattgga gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt
     5281 atcgcatttt cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca
     5341 gtctcatttg acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga
     5401 tacagacttg atgatcgagc agattaagga attgttggaa gggcgtccgg tggacatccc
     5461 gacctacgac tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga
     5521 tgtctttatc gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga
     5581 tatcaagatt tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga
     5641 tatggaggaa cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa
     5701 accaatgtac caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga
     5761 aggggttagc aataccgtgg ctatcgacct gttgacgacc aagatttcaa agattttgga
     5821 agaagctcga aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc
     5881 ctcataatgg tacataaatg gagattttta ggcatatttt tggtataata atacccatga
     5941 aagagcaagt aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga
     6001 ccaagtatct gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg
     6061 gtcaagtttc caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc
     6121 atgcagttat tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg
     6181 aagaaggaat gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct
     6241 actccatcat cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg
     6301 aacaacttaa gaaaaaattg acagaagaag gcctgtttca agaacgctta aagcaagctc
     6361 taccccaatt ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag
     6421 atattatcac gaccgtcagc aggcgattcc caggtgttga catccttctt tatccgacca
     6481 aggttcaagg tgaagaggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac
     6541 gggatgattt ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg
     6601 cctttaacga agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta
     6661 gtgtggggca tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa
     6721 cgccaacagc ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt
     6781 tgcaaaatca ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag
     6841 aggctttgaa aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg
     6901 gttatttgca acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga
     6961 tttctgataa caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg
     7021 ttaccaaaat ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc
     7081 aaatggcgtt agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc
     7141 tcatgttgga tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt
     7201 ccattgtaga ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag
     7261 atggtcaagt agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta
     7321 gcagaactgg aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg
     7381 attactgcct ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag
     7441 gctgaaaaga ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga
     7501 aaaagcaaga aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc
     7561 agtttgcctc tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta
     7621 ttcggccttt tctcttgtta gaagttctgg aagccttgca ggttgccatc aaacctgctc
     7681 acgcgcaggt agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc
     7741 ttcctgctat ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg
     7801 gtgaagctat ggccattttg gctggagatg ccttattctt agacccatat gccttgattg
     7861 cgcaggcaga tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt
     7921 caggtagtct gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact
     7981 tgtctctgga agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc
     8041 ccttccaagc ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa
     8101 ctgtgggtga attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag
     8161 ctagttttga ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct
     8221 atcctgcctt gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag
     8281 ctaatgaaaa attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa
     8341 gtgtagtaga aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa
     8401 caggggttgt ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg tctagtcgta
     8461 gcagtcctta atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa
     8521 ttaaaactca agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag
     8581 gccttgcagg tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct
     8641 actggaggtt ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat
     8701 gttggtacca atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag
     8761 cagttcaatt ttcgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt
     8821 attgatgtga gtttcatttc ccttagtctg attttgccag ccttgcaccg tgtcttggct
     8881 gatcaaggtc aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt
     8941 gggaaaaatg gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca
     9001 gctatggcag tagaggcagg tttttcagtc cttggcttgg atttttctcc catccaaggt
     9061 ggacatggaa atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag
     9121 attcttgctg agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa
     9181 aaaagaaaga cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga
     9241 agaaattgta gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg
     9301 agatatcaaa gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga
     9361 attaccaaaa tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga
     9421 attgatggat aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc
     9481 tcagttaatc gatacttttg cagacaagat ttttagttgt ttgactgatg atagctcgat
     9541 tttagtcatt gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg
     9601 gtaggtctat atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc
     9661 cctcaatttt gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat
     9721 tatcattgat gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca
     9781 tggtgcacca aaggcagaga ttgaggggct tttctcagtt gagaatagtc gccttttaca
     9841 ggaaattttt gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat
     9901 cttgcaaaat ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt
     9961 gcgagctatt gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat
    10021 gcgtccccaa ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt
    10081 gaaagaaacc tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga
    10141 agtcaagaaa aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc
    10201 agagattgag gcagcaaact tgcaggctgg agaagacttg gctctcaatc aagagcgaga
    10261 taaactcctc aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga
    10321 caatgaagat ttttcaagtc tggccaatgt tcgttcagct atgaatgaca tggaaagtgt
    10381 cgaagagtat gatcctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt
    10441 tttagaagac attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa
    10501 tcgcctcatg caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg
    10561 tgggactgtt gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt
    10621 gacaggcaat aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa
    10681 tcttgtcaat ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga
    10741 agctgagatt aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg
    10801 ttttagtaag ggaaaattca gtcgtgaggg aaatgaaatg gttgagtttt atatttcaac
    10861 caaccctgga gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg
    10921 tctcatgtta gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt
    10981 tgatgaggtg gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca
    11041 taaaattggt cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat
    11101 tgcagattat caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt
    11161 tcgtctcttg acagtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga
    11221 tgtgacagaa gcagccctga cgcaagccag agaattgttg agaaacaggg agaaataaga
    11281 tgacagatta ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc
    11341 ttaaaacctg ggatggtcag acccagttgc tctttctagg tgatttgatt gacagaggtg
    11401 aggatagtca ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct
    11461 gtttgtctgg aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt
    11521 atgaccatta tcgtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct
    11581 tggatgcacc agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcgtcagact
    11641 tggtcgaatt cattcgtcaa atgccatttg tagtagagac agacaagtat atatttgttc
    11701 acgcaggtat tgatttgact ttggacgact ggcatgaaac aacagattat aaaaaagtat
    11761 ggcttagaaa accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc
    11821 atacaccggt ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag
    11881 aagatggcaa gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg
    11941 tctttacaga ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg
    12001 aagattagta ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa
    12061 atagataccc tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacagggga
    12121 agatccgtaa cttctctatt atcgcccata ttgaccacgg gaaatcaact ctagcagacc
    12181 gcattttgga aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata
    12241 gcatggatct agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt
    12301 acactgcaaa agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg
    12361 actttaccta tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg
    12421 atgcggctca aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg
    12481 atttggaaat catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagagcg
    12541 tgcgtacaga gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg
    12601 ccaaggctgg tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac
    12661 caacgggtga tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt
    12721 accgtggggt tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga
    12781 ttcagctcat gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa
    12841 aagcggttgg tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca
    12901 agacggttca ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag
    12961 aaccattaca tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg
    13021 aatcaaacaa gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta
    13081 gtcttcagtt tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc
    13141 ttggacttct ccatatggat gttatccagg aacgtttaga gcgtgagttc aatattgatc
    13201 tcatcatgac agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg
    13261 atgtgtctaa cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt
    13321 atgtcaaggc gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc
    13381 agcgtaagcg tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta
    13441 tctatcaaat tcctcttgct gaaatcgtct ttgacttctt tgataaactt aagtcttcga
    13501 cacgtggtta tgcaagtttt gactacgaat tgtcagaata tcgcccatct aagctagtga
    13561 aaatggatat ccttctcaac ggagacaagg tggatgccct cagctttatc gttcataagg
    13621 actttgctta tgaacgtggg aaactcatcg ttgataagct caagaaaatc atccctcgcc
    13681 aacaatttga ggttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata
    13741 tcaaggccct tcgtaagaac gtacttgcta aatgttatgg tggtgacgtt tctcgtaagc
    13801 gtaaactcct tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg
    13861 aagttccgca agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa
    13921 atgcctttcc ttgttcacag gaaatttata taagaatcaa tagtaaagca ggcttagatg
    13981 gtctgctttt ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    14041 taccgtatgt atggttactg acttcgtcag tttcatctac aatctcaaaa ccatgttttg
    14101 agctgacttc gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag
    14161 tttcatctac aacctcaaaa ctgtgttttg agtaacctgc g
//
LOCUS       DAVLWA010000046        13266 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLWA010000046 DAVLWA010000000
VERSION     DAVLWA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.046"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(169..1371)
                     /gene="rpsA"
                     /locus_tag="VYN80_001305"
     CDS             complement(169..1371)
                     /gene="rpsA"
                     /locus_tag="VYN80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1541209.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1533..1604)
                     /locus_tag="VYN80_001306"
     tRNA            complement(1533..1604)
                     /locus_tag="VYN80_001306"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Gln,seq:ttg)
     gene            complement(1614..1694)
                     /locus_tag="VYN80_001307"
     tRNA            complement(1614..1694)
                     /locus_tag="VYN80_001307"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1658..1660),aa:Tyr,seq:gta)
     gene            complement(1744..1974)
                     /locus_tag="VYN80_001308"
     CDS             complement(1744..1974)
                     /locus_tag="VYN80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1541210.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2142..2786)
                     /gene="pcp"
                     /locus_tag="VYN80_001309"
     CDS             complement(2142..2786)
                     /gene="pcp"
                     /locus_tag="VYN80_001309"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1541211.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2801..3724)
                     /locus_tag="VYN80_001310"
     CDS             complement(2801..3724)
                     /locus_tag="VYN80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1541212.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3721..4410)
                     /locus_tag="VYN80_001311"
     CDS             complement(3721..4410)
                     /locus_tag="VYN80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1541213.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4475..5179)
                     /locus_tag="VYN80_001312"
     CDS             complement(4475..5179)
                     /locus_tag="VYN80_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1541214.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5235..6257)
                     /locus_tag="VYN80_001313"
     CDS             complement(5235..6257)
                     /locus_tag="VYN80_001313"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1541215.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6389..8860)
                     /gene="parC"
                     /locus_tag="VYN80_001314"
     CDS             complement(6389..8860)
                     /gene="parC"
                     /locus_tag="VYN80_001314"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1541216.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RTSENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9280..11223)
                     /gene="parE"
                     /locus_tag="VYN80_001315"
     CDS             complement(9280..11223)
                     /gene="parE"
                     /locus_tag="VYN80_001315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1541217.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11359..12000
                     /gene="plsY"
                     /locus_tag="VYN80_001316"
     CDS             11359..12000
                     /gene="plsY"
                     /locus_tag="VYN80_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1541218.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12043..13203)
                     /locus_tag="VYN80_001317"
                     /pseudo
     CDS             complement(12043..13203)
                     /locus_tag="VYN80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3799 a         2966 c         2321 g         4180 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttt atcgctctga acatcaaaaa agaaaggacg
       61 aaatttgtcc tttctcaacc ttagctgact tcaacccact acagttgaca aagagccaga
      121 aaaatccctt gtttagtaaa caaaggattt tgtgaatttt caatttgatt aaagttcgat
      181 atcaccaaac aaatcagcca ttgaaaatcc tgtttgtgtt tctggaagtt cgaaatcacg
      241 cttttcttga cgtcttggac gacgtggacg agcagcacgt ttttcttctt tttgtccttc
      301 ttcttgggct ggacgttctt caagagcttt aatagaaagt gatacgcgtt ctgcatctgc
      361 gttaacttca agaactttaa cttgaacttc ttgaccaact ttaagagctt cttttggatt
      421 ttcaatccgt ttgtgtgaaa tttgtgatac gtgaacaagt ccatcgatac ctggcaatac
      481 ttcaacaaat gcaccgaagt cagtcaaacg tttaactgtt ccttctacta catcaccttt
      541 agccaatttt tgctcaacgc catcccatgg tcctggtact gttgctttaa gtgaaagtga
      601 tacacgtcct tcttcttcgt taagatcaag gattttcact tcaatttctt caccaacagt
      661 tacaactgat tttggtgata cgttacgttc atgtgacaat tcagtcaagt gaaccaatcc
      721 gtcaacacca ccaaggtcga tgaaagcgcc gaagcttgtg atacgagcaa ctttaccagt
      781 tacaacatca ccaacagcca atttaccgaa tacttcagcg cgagctgctg cagtagctgc
      841 ttcaacaact tcacgacgtg aaaggatgaa gcggttttct ttagcgttaa cttctttgat
      901 tttagtatca aattcttgac ctacaaaacg ctcagcgtta cgtacgaaac gagtatccaa
      961 cattgaagct gggataaatc cacgaacacc ttcaaattct actgaaagtc cacctttaac
     1021 ggcacgcgtt cctttaacag taacaacttc ttcttcgcga ccaacaagtt tgtcccatgc
     1081 tttgcgagct tcaaggcgtt ttttagatac aaggtatgta actgtatcag tatctttacc
     1141 aactacttga cgaagtacaa gaacatccaa tacttctcct actttaacaa agtcattgat
     1201 atctgcatca cgatcgtttg tcaattcgcg aagagtcaag acaccttcaa caccagttcc
     1261 agagattgca acgttagctt gagtcgcatc aactgtcaat acttcagcac taacaacatc
     1321 accagtctca acttgactaa cgctatttag caaatcttca aattcgttca tctaaaaaat
     1381 cctccaacaa tcaagttttt cttaaacttg acatacttat aatttttttc ctaagcaccc
     1441 gcaaggacat gttcatatca ttcacgtctt tcaattataa aaataaaata ccctaagata
     1501 ttttgctttt tatcttgatt attacctaag aactggggta gctggattcg aaccaacgca
     1561 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccattaatg aaatggagag
     1621 agagggattc gaacccccga acccgaagga gcggatttac agtccgccgc gtttagcctc
     1681 ttcgctatct ctcctacaat caacatggac tattatatca tgaaaatttc aaaaaaacaa
     1741 gacttatttt gctaaattat aattttcaat caaaatttcc acagcttttt ccaatttttt
     1801 ataaaaacta tcgacattcc cattctttat aatggcaaat tgtccgtcta attcggcaat
     1861 ttctccgata acttttttac cgatagtcaa tgtataacct tcaaaactgt ctttaccaac
     1921 attaactttg gcatccgcta cttgaatttc gattttttta tctttcttgc tcatttcata
     1981 cctctcttca ctttttctat tttacaagaa aatcctaaaa ctagcaagaa aaaactctat
     2041 atcaggcttc tctgatatag agttttatgt aaacaacctc aatcgattta gcatagctaa
     2101 tagttagtaa acaagcataa ttgactatcc tttttaggtt atcagtgagt ctcaccgcct
     2161 actaactgca aatccttatc tttgtagtct acaatagctt caatagctac ctctatgcct
     2221 cttacaatat cacatagact cattcctgca gtattcggct tatttactac ttgttccatc
     2281 atatatggaa tatgcatgaa acctgctctc atattaggga attttttatc cactaaataa
     2341 agagcttggt acatcaaatg attgcaaaca aaggttcctg cactattgga tacaactgcc
     2401 ggaagtccct gttttttgat agcttgtacc atcgctttga taggtaaact actaaaatag
     2461 gccgatgctc catcaatacg aatcggtgta tcaattggtt gattgccttc gttatcaggt
     2521 atgcgagcat catcttgatt aatagccact cgttcaggtg ttaagccggt cctgccgcct
     2581 gcttgtccaa tacaaagtac agcatctggt tgatatcgta atatttctgc ctctaaaact
     2641 tctgacgact tataaaaaac cgttggaatt tctacccagc gaacttcagc cccattaatc
     2701 tcagatggta ataattttac agcctccaaa gctggattaa tcttttcacc tccaaaagga
     2761 ttaaaacctg taaccaatat tttcatttta ttttccttta ctaaaatgcg agaaagtaca
     2821 ttaagaatat gtgaataaca atcattacta gagcaacacc tgcttgagcc tttataacgc
     2881 cattctgatc tttcatatcc atcaatgctg ctggtagagc gttaaaatta gcagccattg
     2941 gggtcaataa ggtcccacaa taacctgctg tcatggcaag agcaccagcc acaattggat
     3001 tagctcccag agcaaataca aagggaactc caacacctgc tgtaataacg gtgaatgctg
     3061 caaaagcatt tcccataatc attgtgaata gaaccattcc aagaacatag gccaaaactc
     3121 ctataaagcg actatctgaa ggaacaatac cgctaatcag atgagagata acatcaccaa
     3181 cacctgctac agtaaaaata gcccccaaag cccctaataa ttgaggaaca ataccacttg
     3241 ttgaaacttg ctgagtcatt cgattatttt ctgataacag actcttaggg tgactattgg
     3301 taatcacaag aacagaaatt gtagcaaaca aggcggcaag gctaatcgaa atcttgctaa
     3361 attctggaat catttgcgct aagaccaacg caagtattgc catcagcata actggaataa
     3421 aaattttatt tttcaacctg ttagattcaa tattggcttt catttcatct aaggatggca
     3481 aggttccgat acggacttgc ttaaacaatg ttaacagcga taataggatt acaataatac
     3541 caatactcat atttggcata taggaaccac ctataaacgt aatagacaat agagtccaaa
     3601 atgcagatgt cccaagtcga actgggtttg ttttatcttt ataactacaa taggctgtat
     3661 ggagaaattg acaaccaatc acaatatagg tcaactctaa tagttgcttt gccaactctg
     3721 tcatttttgt tctcctcccc tagtcttttt tgatatcaat tttttatcaa ataaataatt
     3781 gtaaatcccc actacaataa gtgttataac agcaacaata atagatgtag aagcaatccc
     3841 tgcataattg ctttcatagc ctaactgatc taatgttccc cctatcaaga ggactccccc
     3901 agcacctaca aacgtatttt gagcaaagaa atttccaaaa ttttcattcg cagccgcacg
     3961 cgcttttatt gtctcatctt caacctctgt taactttcta cctaattgag actctgcagc
     4021 tgcttctccc ataggttgaa ccaaaggtct gacaaactga gggtgtcctc ctagacgaat
     4081 tgaaaagaaa ccagctaact ctcgaataaa aaaataaact gtatagaagt ttccaactgt
     4141 cagaccttta atctttcgaa tcaaatcgat tgatcgttgc ttgagtccaa aggtttctga
     4201 cagccccaca agaggcaagg taaccataaa aatcgtgagc actcgctgat tgctaaattc
     4261 ttttcccaaa atctccaaaa attcaacgag agaaacacct gaaactaaag ctgtaaccaa
     4321 accagctaag actactgttg caattgtatc aaattttaaa ataaaaccca caacaatgat
     4381 tgctattcct attaatctaa tccactccat atcaaactcc tttatattca aaatgacagt
     4441 attttaaaaa ttttatcaag atcaatacca ttccttattt aatgtatttt tctagttctt
     4501 tttggtattt gctattggat tccaattttt ctttttgcca ttttttaaaa acctcgttat
     4561 attcttttgt tgtaacaata tctttttgca atttcattcc tttaaagata tatggttccc
     4621 ccttaatacc aacttgtgag tatggttttg agaatggtac tacgttactt acaactggag
     4681 aaccaccaga tgaagctgtt ggcatcaata atgaactatc tgtcgaccaa gcttgagctt
     4741 tggcatattt ttcatatctc ttctctaggt cagtggtctc agaaacagca tcttctaaca
     4801 atttcttata tttatccaaa ccaggtttag ctacaacatc cttatctttt cctttcgtaa
     4861 taccaaggtg tttcatggca gaaccagatt ttggatccat aatattcaag taagacgctg
     4921 gatcttgata gcttggagcc catcctgtac tattcaaatc atagtctttt tgagaaggag
     4981 caacattgcc gtatttatca ttttccatca aaccatcaat aacatttcca ataacgtctg
     5041 tcctcgatgt tcgagtcgct atactgtagc ccaatgatgc tggatctact gcatagacat
     5101 aagaaaatgt tgtcggtgca tctgcttctt tatcagtttt tccacaagcc actaaaatag
     5161 ctgacgtgct caggaccact cctgctgtta agagccactt tttctatttc ataaaaaatc
     5221 tcctttggtt tattttaatc tacttttaca atccaacctt ctggcgcttc aatatcgcca
     5281 aactgaatac ccgtcaattc attatataat ttacgcgtca caggacctac ttctgtttca
     5341 ctatagaata catggaaatc atcaccatgt tgaatacctc caattggaga aataaccgct
     5401 gctgtaccac aggcacctgc ctctacaaaa cggtcaagat tatcaattgg aacatcaccc
     5461 tcaataggag ttaatcccaa gcgatgttct gccaaataaa gcaaggaata cttggtaata
     5521 gatggcaaga tagatggact caatggtgtt acaaattcat tatcagctgt aattccaaag
     5581 aagttagctg atccgacttc ttcaatcttt gtatgagttg atgggtccag atagataaca
     5641 tctgagaaat gacgtgactt ggccattttt cctggtaaga gacttgcagc atagtttcca
     5701 ccaaccttag ccgcacctgt accatttggt gctgcacggt cgtactcatc ctgaatcaag
     5761 aagttggttg ggaccaaacc acctttaaag taatttccaa ctggcatagc aaagatggtg
     5821 aaaatgtact cttctgccgg ttttaccccg ataatatctc cgacaccaat caaaagaggg
     5881 cgaagatata aggttccacc tgttccgtat ggtggtacgt attcttcatt cgcacggaca
     5941 actgctttac aagcttctac aaacatgtct gtcggaactt gtggcatcaa gagacggtca
     6001 catgtacgtt gcagacgttt agcattttca tcaggacgga acagttgaac actgccatcc
     6061 ttagtacgat aagctttcaa accttcaaat gcttgttgtc catagtgaag acttggagaa
     6121 gactctgaaa tatgcaaagt tgcatcctct gtaagctctc cttgatccca ttgtccattt
     6181 ttgaaatgag caagatagcg ataaggtaat ttcatatagg aaaaaccgag gttttcccaa
     6241 tcaatcgtta ctgtcatatt ccactcctta ttctaaaaac ctatttctta tattctacac
     6301 tatttttcta aaatagcaag tatattttgt aattttcaga aaatttctcc aataaaaacc
     6361 agctcttaga actgattctt catttcactt atttatcttc agtaactact tcctgaagat
     6421 aagcgtcaaa aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc
     6481 gttctgacaa gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga
     6541 caagcagagt ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt
     6601 gctctgcaac tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga
     6661 cttgtaatcc tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca
     6721 agcttccacg ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga
     6781 ggacatcatc ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg
     6841 gaacctcttc gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat
     6901 ctagtttaat cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact
     6961 tgacagactt agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga
     7021 tttgaccaag gcgagtcact gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt
     7081 ccacataaag gatttcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc
     7141 cgatgtcctt ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa
     7201 gacttgtgaa catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt
     7261 catcatcacg cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac
     7321 gcttgatgta acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag
     7381 ctgtatcaat ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa
     7441 atttcttctt gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac
     7501 cgataatagc cgccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga
     7561 caaccacatc ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt
     7621 cttccgtaaa gtcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag
     7681 tacggataag agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga
     7741 tatggagacg tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc
     7801 gacggtgagc gatatagcta gacaggattg gaacaatccc aacctgacga ggtgtgaaat
     7861 tgtcaatcgc caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt
     7921 agttgagaac aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat
     7981 cacggtcaga ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat
     8041 catcgatttt cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga
     8101 tttgttcctt accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc
     8161 ctttcccagt ctcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag
     8221 ggaagtctgg tccaggcaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa
     8281 tcatgtaaac tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat
     8341 aaccagccga aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga
     8401 ccgttggttc tttctccgta tcgtcaaagt tccatgcaaa aggaactgtc tttttctcga
     8461 tatcctgaag aaggtagcct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg
     8521 caggaggatc tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat
     8581 ttttccagtt ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt
     8641 ggaaattccc catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag
     8701 tattgctatc cttattcata gaataaagaa tacggcgctg aaccggcttc aacccatcac
     8761 gaatatctgg caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc
     8821 ccatgatgtc ctccagggac atattttgaa tgttagacat aagatacaaa gcccataaaa
     8881 taccaagtga aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt
     8941 cacacagtat ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca
     9001 gtaaaagata ttttacggga attcgtcccg tgttcagtta cgataagtaa ccaaactatc
     9061 ctgtttgtat ttttcaatat gaaaatctgg ttttccaaaa ttagtcttag tttgtgtctt
     9121 agccgctccc ttaaacgcct ctttgagata agcactcata gaagattctt cattaataat
     9181 cctgcaattt tttcaaacca agattttcaa actgcttttt tacatagtca ttcacatccg
     9241 actctaattt ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc
     9301 tagcgtaaac ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat
     9361 gagaacattg acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt
     9421 acgtgtttct gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc
     9481 tttgtatcgt tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc
     9541 gtccgtccaa gcgtaggcca cttcttcttt cttgccttta cctttggaca tcttgtaaag
     9601 aggtgggagg gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa
     9661 aaatgtcaag agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat
     9721 gatcttatca tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt
     9781 ataaatcatg gtattgatct cttcattttt gaggatatcc gccatcttgg ccttggctgt
     9841 attgacaacc ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt
     9901 ggcagaacca ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt
     9961 agattgggct ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc
    10021 atttcggctc tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc
    10081 cttgcggatg aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc
    10141 agccactatt ccatccacaa ctgggcgagc tagggggctt cctagtttat ccttggtctg
    10201 tccttcaaac tgcaagtgtt cttcaggaac taagatagaa agaacggccg ctagtccctc
    10261 acgatagtct gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc
    10321 atagtcattc atgaccttgg taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc
    10381 cttggtgcga acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg
    10441 gagggctact tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa
    10501 gatttcctta tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa
    10561 ctcaatcgct tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa
    10621 ggctgattca ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaagat
    10681 agtcgcgtca ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat
    10741 tttcttcaaa gtcgtgacag gttttccacc attttcgaaa cgttgcttgt aaactgcgcc
    10801 atcacgggta atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac
    10861 tccgtgaagt ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag
    10921 aatggtaaag ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc
    10981 acgtccatgg tcttgaaccg ttagactacc gtctttattg atagttacat cgatacgatc
    11041 accaaaccca gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg
    11101 aagaccagcg ccatcggtcg atccaatata catccccgga cgttttcgga ccgcatccaa
    11161 cccttctagc acctgaatag catcatcatt ataattgtta atattgattt ccttttttga
    11221 cacaaggaac ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca
    11281 aaatttttct ttctccgatg tgacaatttc agcagagatt ctctgctttt ctttcccaat
    11341 tcatgatata ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt
    11401 tcgattccat ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat
    11461 ggttctggta acactggaac gaccaatacc ttccgcattt taggtaagaa agctggtatg
    11521 gcaacctttg tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt
    11581 catctacaag gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc
    11641 cctatctttg caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtgattttc
    11701 ggatttgcgc ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt
    11761 ggcagtatga tttcactgtc tagtgtcaca gcatcgatcg cggctgttat cggggttctg
    11821 ctctttccac tttttggttt tatcctgagt aactatgact ctctcttcat cgctattatc
    11881 ttagcacttg ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat
    11941 aaaactgaaa atttggtccc ttggggattg aacctaaccc atcaagatcc taaaaaataa
    12001 aatgccagtt ctgtactgcc cccaaacagt tagacaaata atttatccaa aggatttagt
    12061 tctgtactgc acaggactaa gtccttttag ttttacctta attcgtttgt tgttgtagta
    12121 atcaatatag tctataatgg cttgttccaa ttggttaagt aatttaaatg ttttctcata
    12181 gccataaaac atttcggatt ttaaaatgcc aaagaaagat tccatcatac cgttgtcttg
    12241 gctgtttccc ttgcgtgaca tagatgcttg aattccctta ctctctagga accgatgata
    12301 agaatcgtgt tgatattgcc agccttggtc actatggaga atcgtattct cgtagtgctt
    12361 ctctgtgaat gcctgttcca acattgtttg tacttgttct aagttgggtg aagttgaaag
    12421 attataggcg ataatttcgc tattaaagcc atctaaaact ggtgataagt aaagcttttg
    12481 agtacttact ggaatggcaa attcagtcac atctgtgtag cactgttcca ttgttttaga
    12541 gccttcaaat tggccttgaa tgagattctc tgcctttttg ccaacgtctc ctttacgaga
    12601 agaatatttt cgtttctgtc gcattttagc ttgtaaattg agtactttca tcaagccttg
    12661 aactctttta tgatttacca gataaccacg atttcttagt tctaaataaa tccgacgata
    12721 accataattt cccttgtgtt cgataaagat ggattgaatt tcagctttaa gctcttggtc
    12781 cttatctggt ttatctagct gtttcaagtg atagtagtag gtcgaacgag ctagtttaat
    12841 ggcttttaga agaatatcta acgaaaactc agtcattaat tcttgaacaa tttctgtctt
    12901 tcttctttct ctttttcctc cttcaatcgg aattctctta acttttttag gatggcattc
    12961 tccgctctca ggtactctcc ctcttggttt ctcaacaata gtatacccgt tttttctgta
    13021 ttgtgctagc cagttaagaa gtatcgtacg acttgggaga ccgtattcaa gagaaactct
    13081 atctttagtc cagccttcat gtcagacttt attaatcatt tcttgtttta aatcaggaga
    13141 atagtaacga ttttttcctt ttttgacgaa ctctattccg taacgatcaa tcaatttaat
    13201 catataccta agattagaat tgtttatccc aaatttattt gaaagcttct ctaagctata
    13261 tccttg
//
LOCUS       DAVLWA010000047        13024 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLWA010000047 DAVLWA010000000
VERSION     DAVLWA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.047"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..239)
                     /locus_tag="VYN80_001318"
     CDS             complement(<1..239)
                     /locus_tag="VYN80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541219.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFP"
     gene            complement(236..589)
                     /locus_tag="VYN80_001319"
     CDS             complement(236..589)
                     /locus_tag="VYN80_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1541220.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            1353..1673
                     /locus_tag="VYN80_001320"
     CDS             1353..1673
                     /locus_tag="VYN80_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1541221.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(1726..2298)
                     /locus_tag="VYN80_001321"
     CDS             complement(1726..2298)
                     /locus_tag="VYN80_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1541222.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            2540..3211
                     /gene="sdaAB"
                     /locus_tag="VYN80_001322"
     CDS             2540..3211
                     /gene="sdaAB"
                     /locus_tag="VYN80_001322"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1541223.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            3220..4092
                     /gene="sdaAA"
                     /locus_tag="VYN80_001323"
     CDS             3220..4092
                     /gene="sdaAA"
                     /locus_tag="VYN80_001323"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1541224.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            4114..4746
                     /locus_tag="VYN80_001324"
     CDS             4114..4746
                     /locus_tag="VYN80_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1541225.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            4796..4873
                     /locus_tag="VYN80_001325"
                     /pseudo
     CDS             4796..4873
                     /locus_tag="VYN80_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5094..6944)
                     /locus_tag="VYN80_001326"
     CDS             complement(5094..6944)
                     /locus_tag="VYN80_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1541226.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(6986..8143)
                     /locus_tag="VYN80_001327"
     CDS             complement(6986..8143)
                     /locus_tag="VYN80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1541227.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            8201..9367
                     /locus_tag="VYN80_001328"
     CDS             8201..9367
                     /locus_tag="VYN80_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1541228.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            9506..9832
                     /locus_tag="VYN80_001329"
     CDS             9506..9832
                     /locus_tag="VYN80_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1541229.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            9819..10412
                     /locus_tag="VYN80_001330"
     CDS             9819..10412
                     /locus_tag="VYN80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1541230.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            10405..11316
                     /locus_tag="VYN80_001331"
     CDS             10405..11316
                     /locus_tag="VYN80_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1541231.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAEDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            11379..12443
                     /locus_tag="VYN80_001332"
     CDS             11379..12443
                     /locus_tag="VYN80_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541232.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(12680..12877)
                     /locus_tag="VYN80_001333"
     CDS             complement(12680..12877)
                     /locus_tag="VYN80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEV1541233.1"
                     /translation="MRSLSQEKDIYSFDEPTGNLDRNSTELFLNEVEKLVNEEKIVIV
                     VTHDKDVIARASKVINMDEFH"
BASE COUNT         3965 a         2845 c         2291 g         3923 t
ORIGIN      
        1 gggaaaaact tattgattaa aatggggaaa ctcattaaca gaatcgaatt tatggctaga
       61 cagatagata ctattttatt tttcacagaa tatagaggta gtgatactat tgagtagata
      121 aaagctaaaa aaataagcaa aaaatactcc gtgtcattta taatcgccct gaatgataaa
      181 aatatataca tccaacatac gataatgtgt atttttcgaa tcttatctac attactcatc
      241 ttactgtcgc agatatgaat ccatcccaag gataagggtt cccgctaata tcacaacctc
      301 tattaagagc accatatcca atacagtaag ctattcttcc tgccgcccca gccaatacac
      361 ctgctgctgc accaataagc caacctccaa ggcctccaac tttaatatag tttattagtt
      421 ttttggttaa aactgcagct ccagctaatg ctataaaagt tcctggactc atagctaaag
      481 ctgcgcaaat tccttgacaa gcacgcttgc ttaaataagc tcccccatca aggtacatca
      541 tctcttcttc atcaatcaca acataattat ttggtaatac aagttccatt tattttctcc
      601 ttttttatta agcatggtta ggatggtttc ctaacaagtg atcgcaggct ttgtttagtt
      661 acctaaatta gacttccttt ctttatctta ttttaaatat ttaaaaccag ttactgcttg
      721 aagcttaaca gtttacttaa ttcaaataac agctaatcac tgattccctg attttatcaa
      781 atatactaat aacaacaatt gtgatacgat catcattata agaacaacat ttaacgaatc
      841 aaagtttttc cgagataata ataaagcaac agcaagcagt attattacta aagattgaat
      901 aactactatt actcttaaca ttttatcctc cactaacaac tcaagctccc ttacattata
      961 tcgaccagcc ccatggaaag attatagcta gactaatcaa tgatataata catactagaa
     1021 tacaaaatat ttttttattc atactttttc tcctatcttt attttctacc tacatagtac
     1081 ctcttattaa gctcatttca aaattacgtt aacttttttc acgaaaagga gaaaattatg
     1141 cgttatgatt atggatagcg ctttcaaatt tttaaactcc tatcccgtcc ttttatctat
     1201 ataataagca taagcgaaaa tataataact gtcaagtaac tgaagtgaat tttataaaaa
     1261 aattacaagc caaatttgta aagtttacac taagccgcta gggcaatcgt ctatcagaat
     1321 gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc tataaaaact
     1381 atcaacattt atatgacttg cgaatgacaa ttctattaaa cctttccaca ctctatctat
     1441 acaatcaaga taaaaacatg tgtaagcaaa tctgctacac tttactggag gacgccaaga
     1501 ataagaaaag ctacgatagg ctggctatct gctatgtccg tattgggatt tgtagggatg
     1561 atgcgaaact tatccaaaaa gggttctcac tcctagagct gaccgaggaa acttctatgc
     1621 tgtctcatct caaaaaagaa gtagagaccc attatcaacc aaagaaatta taaaaaaagt
     1681 cgagggattt cctcgactct ttacatatta tttgttcgtt agttcttaat accagccatt
     1741 gttaagccag aagtttttag cagcagtcca tgaaccgtaa cgtcctgcaa cgtaggcatc
     1801 tgctacacgt tcttggtttt cagctgagta gtcaccgttc aggtatgaat ctgttaattg
     1861 gtaacgtccg atataacgtc catttgtagc tgtatagcta ccacctgatt ctttttgagc
     1921 gatccattct ttggcttctg cttcagatcc gcttacagtt gaaacaactg tttcacttac
     1981 tactggagct gaaacagttt catcttgagc ggctggtgcc gcataagtag ctggcgctgg
     2041 tgttgcaaca ggcgctacag ggccatcgat aaccaactct tgatcaacat aaatcaaatg
     2101 aatgttatca atgtggttgt tttctgccaa tttttcaact gttgtgttgt gagtttcagc
     2161 gatttctgaa agtgtatcac cttctttaac agtgtaagtt gatgattctt gagcagatac
     2221 aaatgatgga gcaaatactg caaacaaggc agctactcct gcaagagttg ctttaatctt
     2281 tttagttatt gatttcatat tcgaaaattc tccttctttc tatagttcta tcataccctt
     2341 taaatattac cgtttcttga cacttttatg tagaaatatt acaaaattat tttttatttc
     2401 cctaaataag gtgttttttg tcataaaaga tactattttt atgctatttg aggtattaga
     2461 aaggttttca tccacaatta aagccaagcc cctcattctt tgatataata gagataagaa
     2521 tttttataag gggaaactga tgaaatcact tcgttttcaa tctgtctttg atatcatcgg
     2581 accagttatg attggcccat ctagtagcca taccgctggt gctgttcgta ttgggaagat
     2641 tgtctcttcc atttttgatg atactccgac agaagttgaa ttccaacttt ttaactcatt
     2701 tgccaagacc tatcgtggtc acggaacaga cctagccctt gttgcaggta ttttaggaat
     2761 ggatacagat gatcctgaaa ttccaaatag tctggaaatt gcccacaagc gtggtatcaa
     2821 gattgtctgg accattcaga aagacagcaa tgcccctcac cctaacacca ctaaaattac
     2881 tgttaaaaat gcccacaaga ccatcagcgt gactggtatt tctatcggtg gaggaaatat
     2941 tcaggtaacc gaactcaatg gttttgccgt ctctctcaat atgaatacac cgactatcat
     3001 catcgttcat caagatattc caggtatgat tgcccttgtt acagaggcgc tttcccgcta
     3061 tggtatcaat atcgcccaga tgaatgtcac tcgtgaaaaa gctggtgaaa aagccattat
     3121 gattatcgaa gttgacagtc gcaactgtga tgaggctatc gaagaaattc gaaaaatccc
     3181 tcatctccac aatgtcaatt tctttaaata ggaggaagca tgttttattc tatcaaagaa
     3241 ttggtcgagc aagcagatct agactttcaa ggaaatgtcg cagaactcat gattacaaca
     3301 gagtttgaat tgaccggtcg cgaacgtgaa gaagtcttcc ttctcatgga acgcaatctg
     3361 gaagttatga aagcctctgt ccaacttggc ctcaatgaaa ataaatctcg tagtggcctg
     3421 acaggtggag atgctgccaa attggatcac tacatcaaaa acgaaaaaac tttatcagat
     3481 tacacgattc tctctgctgc ccgaaatgct atcgcagtca atgaacacaa tgccaaaatg
     3541 ggcttggtct gtgccactcc aaccgctgga agtgctggct gtctcccttc cgttctcact
     3601 gctgctattg aaaaattaga cctcagccac gagcaacaat tggatttcct ctttgctgct
     3661 ggtgcctttg gactagtcat cgcaaacaat gcctccatct caggtgctga gggtgggtgt
     3721 caagctgaag ttggttcagc ctctgctatg agtgctgccg ccttgactct ggctgcaggt
     3781 ggaacacctt atcaggccag tcaagctatt gcctttgtca ttaaaaatat gctaggcctc
     3841 atctgtgacc ctgttgcagg tttggtcgaa gttccctgtg tcaaacgtaa tgccatggga
     3901 gctagctttg ctttcatcgc agcagacatg gccttggcag gtatcgaatc taaaatccct
     3961 gtggatgaag tgatcgatgc catgtaccaa gtaggagcaa gcatgccaac tgcctttcgt
     4021 gaaacagctg aaggtggact cgctaccacc cctactggtc gtcgcctcca aaaagaaatt
     4081 ttcggagaat aagcttatca aataggagaa accatgacct ctatcacagc aatttttttc
     4141 gatttggatg gaaccctcgt tgatagttct atcgggattc acaatgcctt tacctatacc
     4201 tttaaagagc tgggggtgcc tagccctgat gccaaaacta ttcgtggttt tatgggacca
     4261 cctctcgaaa gtagttttgc gacctgcctg tccaaagacc aaatttctga agccgtgcag
     4321 atatatcgtt cttactataa ggcaaaaggc atctatgaag ctcaactctt tcctcagatt
     4381 atagacttgc ttgaggagtt atcgagcagt tatccactct acatcaccac gacaaaggat
     4441 acatctactg ctcaagacat ggcaaaaaac ttggaaatcc atcatttctt tgatggcatt
     4501 tatggttcta gccctgaagc accccataag gcagatgtca ttcaccaagc cttgcagaca
     4561 catcaactag caccagaaca agccatcatc atcggagata ccaagtttga tatgctggga
     4621 gctcgagaaa caggtattca gaaattggcc atcacttggg gatttggaga gcaagcagat
     4681 ctactaaact atcaacctga ttatatcgct cacaaaccct tagaagtttt ggcgtatttt
     4741 caatagcata gactgggtaa caattccatt tcagaagaaa atgaagtaat actcaatgaa
     4801 aatcaaagag caaactagga aactagctgc gggctgctca aaacactgtt ttgaggttgc
     4861 agatagaact gacgaagtca gctcaaaaca ttgttttaag gttgtggatg gaactgacga
     4921 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gtaaccatct
     4981 acggcaaggc gacgttgacg tggtttgaag agattttcga agagtataag tctctacata
     5041 ataaaacgca tattaccaag gttgtgtaac ttgatactat gcgtttttct tttttattca
     5101 atgtgggttg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
     5161 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
     5221 acgaaaatct tatccataac ttgattatca acgagttcgg ttgataccaa gagttcttcg
     5281 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtgtgg
     5341 ccacttagaa gcaccatctt cttggccaag tcataaatct tgactggttt gcccatatca
     5401 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
     5461 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
     5521 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
     5581 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
     5641 gccacgcgct tggttgctcc cataacattt ggtggattga ctgccttatc tgtcgaaatc
     5701 ataaccatct tagacacttt agcttcatca acagccttag caacattgta agttccacgg
     5761 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggca
     5821 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
     5881 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
     5941 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     6001 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     6061 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     6121 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     6181 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     6241 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     6301 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     6361 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     6421 gttggatgtt ggtaactatc cataaaaaga gccccaccat caccggcacc aatcaagaag
     6481 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     6541 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gatgaaacgg
     6601 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     6661 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat gctcgcgttc
     6721 aacccccaaa aaccaatcat caattgatag aacaagaagg ccaaactcgt atagataatg
     6781 tagtcaacag gtgctggatt aatcagccca taaaataaaa tataagatac aatgatggaa
     6841 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     6901 atttccacca gatcaatcac ataatctgtt agttttttat tcatagaaat ctactcctcc
     6961 ttaaaagagt tttactatat tgttattata gcatattttt atttatttgg cttattttat
     7021 ccaaataaat tattgttctc ttgtactcct tcttccattt aaatcctcta attaaaccta
     7081 caatcgtccc aaggccataa gcaaagtgaa tggaaaataa aagaaagggc atcacaatta
     7141 gaaatccatt tttatgtttt aataaagtca gcaaagtgag taatgacaaa agtagaaaat
     7201 aggcacctaa tagtaaagtt atgaatacga atgtgatcgg taacaatgct agactaaaca
     7261 caagactcaa aacaaataaa caaggaacat agtgaaataa tgataaacac ttaaactgaa
     7321 catgacttgt caagccaatc cacaaaccat ttgaatactt ttgatgcagc attttcttga
     7381 atgttggtcg aatatactga taagatagaa tacttgggct atagcggatt ttataaccat
     7441 gttctcgaat tctataatga atatcattat cttcagttcg gccaagttgc tcatttacta
     7501 aaccaacctt ctggaaaacc tctcgtttat acatcccatg aaaaatagaa gaaacatatc
     7561 tatcctcgga actatttcga taattggcaa tgctactgcc aaacatattt tcctcaacaa
     7621 gatgcaaggt ctctgcccat tttccttttc cttcgacaat cgtcggtcta ggccccccac
     7681 agacaaattc accttgttga ataatagcca cattgttcat tacaaaagtc tcagtaactt
     7741 ttgaatgagc atcaattttt aaaataaggt cccctacaga atgtttaact cccaggttaa
     7801 aaccactagc ttgatttttc ttaggattgt tatacaatct aattgagtta aactctgtat
     7861 cttcctttat aaattgctga atgatagctg tggtcccatc tgtggacata gcatttataa
     7921 atagaatttc aatattctct ttaggataag tttgattttt taagtcttca attagaccag
     7981 gcagatattt ttcttcattg tatgcactga ttacaatgga tacgagaaga ctttcaacat
     8041 tttgagtcac tactactctc ctgaattttt ccttccacca ttctatcata attttcaaga
     8101 ctttcccatt tttatcttga aagccagaaa ccttacttga cattatagtg aaaaagcctt
     8161 aaaataagct gttattaaaa tcagctagaa gaggtattat atgaaaaagc aatcactctt
     8221 ttttgttcca ggtattatcc tgattggtgt ttccttgcga actcctttca ctgttttacc
     8281 cattattttg ggaaatattt cgcaaggtct ggaggtagaa gttagttcgc ttggtgtctt
     8341 gaccagcctg cctctcctta tgtttaccct cttttcacca ttttctaccc aactggctca
     8401 gaaaatcgga ttggagcatc tcttcaccta cagcctcttc ttcttgacca tcggctcact
     8461 cattcgacta atcaatctgc ccctgctcta tctaggaacc ttgatggttg gggcaagtgt
     8521 cgcagtcatc aatgtcctgc ttcctagtct tatccaagcc aatcaaccaa agaaaattgg
     8581 ttttctgacc accttatatg taacgtctat ggggattgca acggctctgg cttcctatct
     8641 agctgtgccc attacacaag ccagttcttg gaaaggactt atcctcctcc tcacgttact
     8701 ctgtctagca acttttttgg tctggctccc aaatcaccgc tataatcata gactagctcc
     8761 acaaaccaaa caaaaaagtc aaataaaggt catgcgtaat aaacaggttt gggcaattat
     8821 tatcttttca ggttttcaat ccttgatctt ttacaccgcc atgacctggt tacctaccat
     8881 gtctatccat gcaggtctat ccagtcacga agctggcttg ctgacttcta tcttatctct
     8941 gattagcatt cctttttcaa tgaccatccc aagcctgaca accagtttat ctactcgcaa
     9001 ccgtcagctc atgctcactc tggtttcact agctggtgtg gtcggcattt ccatgctctt
     9061 tttcccaatc aataatttca tttactggct tgccatccat ctcctcatcg gaaccgcaac
     9121 cagcgccctc ttcccttatc tcatggtcaa cttttcactc aagacaagcg cccctgaaaa
     9181 gacagcccaa ttgtccggcc tatctcaaac aggaggctat atcctagcag cctttgggcc
     9241 aaccctcttt ggttacagtt ttgacctatt ccactcttgg gtaccagctg tagctgccct
     9301 cttgctcatc gatatcctga tgactgtggc cctctttaca gtggacagag cggataagat
     9361 cctttaaact tctagtttta gctagaagtt ttttatatat aaaaatttta cacatttctc
     9421 ttgacagggc tttcttttag atgtacaatg tatgtgtaga aaaattatat ataaaaatct
     9481 tacacattag aaaaggaggt ttcccatgta ctttccaaca tcctctgcct tgattgaatt
     9541 tctcatcttg gctgtactgg agcagggtga ttcttatggt tatgagatta gccaaaccat
     9601 taagctgatc gctaatatca aagaatccac actctatccc attctcaaaa aattggaagg
     9661 caatagcttt ctgacaacct attctagaga gttccaaggt cgcatgcgca aatactactc
     9721 cttgacaaac ggtggtatag agcagctctt gaccctaaaa gatgaatgga cactctatac
     9781 agacaccatc aatggcatca tagaagggag tatccgccat gacaagaact gaatacctga
     9841 ctcagctaga actctatctc aagaaactac ctgaagctga ccgtatcgaa gccatggact
     9901 atttcagaga gctctttgac gatgctggag tcgaaggaga agaagaactc atcgctagtt
     9961 tgggaactcc caaagaagtg gcccacgaag ttctatccaa tcttctcgat aaaaaaatca
    10021 atgaagcacc cgctcaaaaa aataaccgac aaattttaca tatcgccttg ttagccctcc
    10081 ttgcagcacc tatcggcatt cctctgggaa tcgccatcct cgtgaccctg ttcgcaatcc
    10141 ttgtagccgc tttgactgtc attctggctt tctttgcagt ttccatactg ggtatcatcg
    10201 gcggattcct atttttagtt gaaagtttca ctgtcctcgc ccaagccaaa tcagccttta
    10261 tcttgatttt tggttctggt ttactggcta tcggtgcttc ttcgctagtt ttacttggca
    10321 tttcctatgt agctcgcttc ttcggtctac tcattgttcg tctggtacaa tttgttctta
    10381 aaaaaggaaa gagaggtaat cagcatgcgt aaatggacaa aaggatttct catctttggt
    10441 gtggtgacta ccgttatcgg ctttattctg ctttttgtag gtatccaatc tgacgggatt
    10501 aagagcctac tttccatgtc caaagaacct gtctatgata gccgtacgga aaagctaacc
    10561 tttggcaagg aagtcgaaaa cctagaaatt actctccacc aacacacgct caccatcaca
    10621 gactctttcg atgatcaaat ccacatttct taccatccat ctctttctgc tcaccatgat
    10681 cttatcacca atcagaacga tagaactctg agtctcactg ataagaaact gtctgaaact
    10741 ccgtttctct cttctggaat tggtgggatt cttcatatcg caagtagcta ctctagtcgt
    10801 tttgaagaag ttattctccg actaccaaaa gggagaactc taaaagggat caacatctca
    10861 gccaatcgcg gacaaaccac catcataaat gctagccttg aaaatgcgac cctcaataca
    10921 aacagctata tcctccgaat tgaaggaagt cgtatcaaaa acagtaaact cacaacgccc
    10981 aatatcgtta atatctttga tacagttctt acagatagtc agctagagtc aacagagaat
    11041 cacttccacg ctgaaaatat ccaagtccat ggtaaggttg aactgactgc cgaagattat
    11101 ctcagaatca tcctagacca gaaagaaagc caacgaatta actgggacat ctcaagcaac
    11161 tatggttcta tcttccaatt cacaagagaa aagcctgaat caagaggtac ggaattaagc
    11221 aacccttaca aaactgaaaa aaccgatgtc aaggatcaac tcattgcgag atctgatgat
    11281 aatattgatc taatatccac accaagcaga cgttgacaaa aacgcttaca tctgctacta
    11341 tggaagcata tttatctaac taaaaaggag gttctaccat gaaacaagaa tggtttgaaa
    11401 gtaatgattt tgtaaaaaca acaagcaaga acaagcctga agagcaagct caagaggttg
    11461 cagacaaggc tgaagaaacg atagccgatc tcgatacacc aattggaaaa aatactcaag
    11521 tagaggagga agtctctcaa gctgaagtcg aactggaaag ccagcaagaa gagaaaattg
    11581 aaactcctga aggcggtgaa gcgagaacag aaatagaaga aaagaaggca tctaattcta
    11641 ctgaagaaga gccagacctt tctaaagaaa cagaaaaagt cactatagct gaagagagcc
    11701 aagaagctct tcctcagcaa aaagcaacca cgaaagagcc acttcttatc agtaaatctt
    11761 tagaaagtcc ttatatcccc gaccaagctc caaaatctag ggataaatgg aaagagcaag
    11821 tgcttgattt ttggtcttgg ctagtggaag cgatcaaatc tcctacaagt aagttggaaa
    11881 caagtatcac acacagttac acagcctttc tcttgctcat tctgttttct gcatcttcct
    11941 ttttctttag tatctatcac atcaaacatg cttactatgg acatatagca agcattaaca
    12001 gtcgcttccc tgagcagcta gctcctttaa ctcttttttc tatcatctct atcctagtag
    12061 cgacaacact cttcttcttt tcattcctct tgggtagttt cgttgtgaga cgatttatcc
    12121 accaggaaaa ggactggacg ctagacaagg ttctccaaca atatagtcaa ctcttggcaa
    12181 ttccaatctt cctcactgct attgctagtt tctttgcttt ctttgatagc ctacgattta
    12241 cggccctctt gtgtgtgatt agcattggaa tcattctgct tgccagtctc catatcatta
    12301 caagaccaag tcaagcaagt gaaaccgact ccttctatca attattcttg tctgtccttg
    12361 tgaacggagt tattatcctc ctcttctttg tagctgaagt ggcactgatt ggagattatc
    12421 ttcgtatctt ggcctttctt taaaattcaa tcctgtcatt cttggcagga ttttttctat
    12481 gtcaaaaaag caagtcgatt gacctgcttc tgctttactt ttcttgtgct aatgttttaa
    12541 aatcttgtcc taggatgggt tttattccta gagttcaaag tatgatagcg ttgctataaa
    12601 gttcgttatt ccaaatatta tgcaaactag ttatatgtaa aattaagatt agaaaccacc
    12661 aatctccatt tattaacttt taatgaaatt cgtccatatt aataacttta cttgctctag
    12721 caataacatc cttgtcatga gtcacgacaa taacaatctt ttcctcgttc actaatttct
    12781 caacctcgtt tagaaataat tcagttgagt ttctgtccaa atttcctgta ggctcatcga
    12841 atgaatagat atccttttct tgcgataaac tcctcatgaa tagaattttt tgtttctgac
    12901 cttacgaaat attggtggct ttcccatcaa tctgatgact cagagctata ttgagattca
    12961 aactatcaag attatttgat tcatagtttt ccttatcaaa tagggtgaaa ttaatatttt
    13021 ttgt
//
LOCUS       DAVLWA010000048        12926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLWA010000048 DAVLWA010000000
VERSION     DAVLWA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.048"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(98..2356)
                     /locus_tag="VYN80_001334"
     CDS             complement(98..2356)
                     /locus_tag="VYN80_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1541234.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VYN80_001335"
     CDS             complement(2585..2815)
                     /locus_tag="VYN80_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1541235.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VYN80_001336"
     CDS             3005..3688
                     /locus_tag="VYN80_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1541236.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VYN80_001337"
     CDS             3688..4422
                     /locus_tag="VYN80_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1541237.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VYN80_001338"
     CDS             4576..5433
                     /locus_tag="VYN80_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1541238.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5570..6391
                     /locus_tag="VYN80_001339"
     CDS             5570..6391
                     /locus_tag="VYN80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV1541239.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            6405..7277
                     /locus_tag="VYN80_001340"
     CDS             6405..7277
                     /locus_tag="VYN80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1541240.1"
                     /translation="MKVIDQALLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7559..8242
                     /gene="rpiA"
                     /locus_tag="VYN80_001341"
     CDS             7559..8242
                     /gene="rpiA"
                     /locus_tag="VYN80_001341"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1541241.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            8256..9467
                     /locus_tag="VYN80_001342"
     CDS             8256..9467
                     /locus_tag="VYN80_001342"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1541242.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            9469..10011
                     /locus_tag="VYN80_001343"
     CDS             9469..10011
                     /locus_tag="VYN80_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV1541243.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            10027..10836
                     /locus_tag="VYN80_001344"
     CDS             10027..10836
                     /locus_tag="VYN80_001344"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1541244.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRTYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            11312..12292
                     /locus_tag="VYN80_001345"
     CDS             11312..12292
                     /locus_tag="VYN80_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1541245.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHT
                     NFQSKPWWQVDLAKEETIRQINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            12317..12829
                     /locus_tag="VYN80_001346"
     CDS             12317..12829
                     /locus_tag="VYN80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1541246.1"
                     /translation="MSAQITINHKKARYVRIELEGYNAFSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3834 a         2325 c         2923 g         3844 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata cggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggtccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt catccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgtccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc caacttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg agggcaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ttaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag cttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc agcaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatac aatcaggatc cagcttggca tgggattttg
     4861 gttcagttgc cattaccaaa acacattgat gaagaggcgg ttctattggc tattgaccca
     4921 gaaaaggatg tggatggttt ccatcctcta aatatgggac gcctttggtc tggacatcca
     4981 gtcatgattc catcaacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgaa
     5041 ttagaaggta aaaatgcggt tgtcatcggt cgttctaata tcgttggaaa acccatggct
     5101 cagcttcttt tggccaagaa tgcaacagtg actttgaccc actcacgcac ccataatctt
     5161 gctaaggtgg ctgctaaagc agatattctg gtggtcgcaa tcggtcgtgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggta gtcattgacg ttgggatgaa tcgtgatgaa
     5281 aatggtaagc tctgtgggga tgtggattat gaggcagttg ccccacttgc tagctatatt
     5341 acgccagtcc ctggaggtgt cggccctatg accattacta tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaaa atcgctgaag agagtgtatt
     5461 ttctatagct atatttaaaa tggcagaaat gaatattaaa ttttagatat aagattacaa
     5521 aaggaggtct gcgcctcctt tttgttgtat aatggagtga ggtgattaga tgattttaaa
     5581 aatttataat agggaatata gtttacaatg ggatggaata tactacctag cactaattga
     5641 ttatccaaat attcaagagt gggaattaga aaaaattgct aaatttatag cttacgaaaa
     5701 acttcataaa cgtcaaacaa gtattgagtg tgctgattct tgtttaaaaa aagaaatttt
     5761 agattacatc tgtcagcatc cctttctgcc accatttacc cctacagata aaagagtagc
     5821 ctcgacttat gatctacata agaggttagt gacttcagac tactgtagtc atactacgac
     5881 tatagatgca gcgatttcta tctttaaaac aggtcagctc ttatctgctg tgaaagcctt
     5941 taggcgagat gctgaagagt tggttttgga tagtagaaat gctgcatcgg atccgataga
     6001 ttattttgat tatgtcatgt tagggtggtc aaatacaagt tctggttatc gattagcgat
     6061 ggagcgttta ttaggtcgag ctccttcaga gaaagaatta caagacaagt ttattcctgg
     6121 agtgagtttt cattttatct atacagattt gattaaagtt actggttata tttttgatgg
     6181 ctaccatgtt gcaaaaatta aggacatgtt gaatttattt agtgagttgt atatttgtat
     6241 tattccaact cataataaga gccaatttga aaatattatt ccaactaaaa tacaagatag
     6301 ggtgcattat cttgactatg ctggagaaga cttagaagag tggactaaga aagtctatca
     6361 agttgtttta aaacaatcag ataaaggata gttgaggaag aacgatgaaa gtgattgatc
     6421 aagccttact agaaaaagtt attattgaac gttctcgttc aagtcataaa ggagattatg
     6481 gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc atggctgctt
     6541 tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg gaaaatatcc
     6601 cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac cagcaattgt
     6661 taaaagagca attggagaag gcagaagttg tcttgctggg gcctggttta cgagacgatg
     6721 cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat cagattttga
     6781 ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt ccctcaagcc
     6841 agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt acgattgaaa
     6901 agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga acaattttgg
     6961 tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat taccagttac
     7021 aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct gggatgatcg
     7081 caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca gtagcgaccc
     7141 atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc ttaccgacgg
     7201 aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag agggtaagca
     7261 aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat ttatgattct
     7321 tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt tgtttttgta
     7381 actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta ttcgtttggt
     7441 aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt gatgttttta
     7501 cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag gtaaatcggt
     7561 ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg atggaatggt
     7621 cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg gtcgtcgtat
     7681 caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca ctaaacaggc
     7741 agaaggcctt aatatcccgc tcaagtctat tgaccaagta gattttgtcg atgtgacggt
     7801 tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg gtggtgccct
     7861 tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg tggatgaaag
     7921 caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc agtatggtgc
     7981 agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc gtgaaaaaga
     8041 cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct tggatgtcat
     8101 tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg tggagcatgg
     8161 tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag ttcagatttc
     8221 aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat cgtattcatt
     8281 tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac tttgtcaatg
     8341 caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca gttggtttga
     8401 atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact cctcttaaga
     8461 ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa gtatctcttg
     8521 gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact gagcctttcg
     8581 atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa ttctcaggac
     8641 gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc gatgattttg
     8701 gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac cctgttttgc
     8761 agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc tgtgaatacg
     8821 ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct cgcccttatg
     8881 taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct gtatctccat
     8941 tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat gctgtgggta
     9001 aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac aacaagtcaa
     9061 atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt gaaaaaggat
     9121 tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt aatgctcacg
     9181 gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc gcagctatga
     9241 gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg tatgcaggaa
     9301 cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt aaaggaaatg
     9361 gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat aactttggtg
     9421 tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat gacgcgctat
     9481 gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat ggcggagctt
     9541 cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa tagtggcaat
     9601 attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga gactttcttt
     9661 gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga ctttgaggcg
     9721 gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga ttttctcttt
     9781 tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt agagctgaaa
     9841 gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc tgaagaatcc
     9901 tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg ccacattact
     9961 attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata ataaaggaga
    10021 cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa agaaaaggga
    10081 attgcagccc ctgagttcgg tctaatcctt ggatcaggac ttggagaatt ggcagaagaa
    10141 atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg ttcaacagta
    10201 gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt cttggctctt
    10261 caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt cccagttcgt
    10321 gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg cggtatcgga
    10381 tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg gcaaaatcca
    10441 ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc taggacctac
    10501 acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat caagcttgat
    10561 gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga aattcgttcc
    10621 tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt tatcgtggca
    10681 gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc ggctggtttc
    10741 caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa aggtgatttc
    10801 aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa aagggggagt
    10861 gcctctgtct tttcaggatt gactgcctat ccggattaaa gaagaaacag aggaatacta
    10921 tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt ctgatctgat
    10981 agccagcatt gtgaaagaca agattctagg atactagcat tagcttccta gccaagtaga
    11041 ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag tcttatcata
    11101 tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga aagataagaa
    11161 ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt atcggagttg
    11221 tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca atgaagtaaa
    11281 gcaagatgta acatctgaag tggtaatagg tgtgctagat tctaaggagg aattgaaaga
    11341 gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa gactagctcc
    11401 tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt caaaagtaga
    11461 gataacagag ccagctcaag cggatgatat ccgcaaggtt gttggggaat tagccaagga
    11521 tataagtatt actaagttgt atatgacagg tcattctctt ggatgctacc tagctcagat
    11581 tgcagaggtt gaagcttacc aaaaatatcc tgatttttat aaccatgtat tgaggaaagt
    11641 gacaactttc agtgctccta aagtgattac ttccagaact gtttggaatg ctaagaatgg
    11701 tttctgggat gttggtttgg aaagtcgtaa attagctgtt agtggaaaaa ttaagcatta
    11761 tgtggttgat aatgacaatg ttgtgactcc cttgattcat aatgatcgtg atattgttac
    11821 atttacaggt aattcacgct ttaaacaccg ttctcgtggc tattttgaaa gtccaatgaa
    11881 tgatattcct aactttaata ttggtaaaca agctaccttg gataaacatg gttatcgtga
    11941 tccgaaattg gataaagtgc gattctttaa gaaacaggct ctgcctcgat cttctagtca
    12001 accaagcgct gaaccaatgg aaaatattgc cttaggaaaa caggttactc aaagttcgac
    12061 agctttcgga ggagatgcta gaagagctgt ggatggcaaa gtcgatggta actatggtca
    12121 caattctgtc actcatacaa acttccaatc taagccttgg tggcaagtag atttggctaa
    12181 agaagaaacc attcgccaaa tcaatattta caaccgaaca gacactgccc aggatagatt
    12241 ggcaaacttt gatgtcattc ttttagacag ttctggtaaa gaaattgagt gaaaacgtat
    12301 aacatctcct aaagatgtgt cagcacaaat tacgattaac cataaaaaag cgcgctatgt
    12361 tcggattgag ctagaaggct ataatgcctt cagtcttgca gaagttgaag ttttccgctt
    12421 tatagctacg aatggtgaaa cggcgataca agtttctaag tcagttcaac caatcagtca
    12481 gactcctgtg aaggataaaa cattgacaat tcaacacagt ggagcttaca ttgcccgcta
    12541 ctccataact tgggaagaag ttccagtaga taaagatgga aaccaagttg ttcgtagtca
    12601 ttcttgggaa ggaaacggtc gcaaccagac tgcaggtttt gtcctcaacc tcccaatcaa
    12661 agaaaatatg agaaatctgc gagttaagat tgagaaaaag acgggcctac tatggaatag
    12721 atggcaaaca atctatgaaa acagaccaat tttagctcaa ccccaccgta aaattaccca
    12781 ttggggtacg acattgaatt ccaaggtgag tgacgatgat gtcttgtaat ctgatggtac
    12841 aatgatagtt agtttgtcta gtttataaga aagtactacc tgagcttgaa taggactcag
    12901 gtagctctct gtgaaagaac aaaatt
//
LOCUS       DAVLWA010000049        12876 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLWA010000049 DAVLWA010000000
VERSION     DAVLWA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.049"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            5..217
                     /locus_tag="VYN80_001347"
     CDS             5..217
                     /locus_tag="VYN80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1541247.1"
                     /translation="MTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLPVHG
                     FCSRGTYDLGYIGGGNHLCKGSAARF"
     gene            271..942
                     /locus_tag="VYN80_001348"
     CDS             271..942
                     /locus_tag="VYN80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1541248.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(994..1191)
                     /locus_tag="VYN80_001349"
     CDS             complement(994..1191)
                     /locus_tag="VYN80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541249.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            1184..2092
                     /locus_tag="VYN80_001350"
     CDS             1184..2092
                     /locus_tag="VYN80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1541250.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2089..2550
                     /locus_tag="VYN80_001351"
     CDS             2089..2550
                     /locus_tag="VYN80_001351"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1541251.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2540..3427
                     /locus_tag="VYN80_001352"
     CDS             2540..3427
                     /locus_tag="VYN80_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1541252.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3430..5313
                     /gene="cbpE"
                     /locus_tag="VYN80_001353"
                     /gene_synonym="pce"
     CDS             3430..5313
                     /gene="cbpE"
                     /locus_tag="VYN80_001353"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1541253.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5393..6523
                     /locus_tag="VYN80_001354"
     CDS             5393..6523
                     /locus_tag="VYN80_001354"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1541254.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6533..7795
                     /locus_tag="VYN80_001355"
     CDS             6533..7795
                     /locus_tag="VYN80_001355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1541255.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7799..8596
                     /gene="proC"
                     /locus_tag="VYN80_001356"
     CDS             7799..8596
                     /gene="proC"
                     /locus_tag="VYN80_001356"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1541256.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8816..9454
                     /locus_tag="VYN80_001357"
     CDS             8816..9454
                     /locus_tag="VYN80_001357"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1541257.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9451..10341
                     /locus_tag="VYN80_001358"
     CDS             9451..10341
                     /locus_tag="VYN80_001358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1541258.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10381..10698
                     /gene="yabA"
                     /locus_tag="VYN80_001359"
     CDS             10381..10698
                     /gene="yabA"
                     /locus_tag="VYN80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1541259.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10701..11570
                     /gene="rsmI"
                     /locus_tag="VYN80_001360"
     CDS             10701..11570
                     /gene="rsmI"
                     /locus_tag="VYN80_001360"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1541260.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11797..12315
                     /locus_tag="VYN80_001361"
     CDS             11797..12315
                     /locus_tag="VYN80_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1541261.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            12424..12561
                     /locus_tag="VYN80_001362"
     CDS             12424..12561
                     /locus_tag="VYN80_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1541262.1"
                     /translation="MDFEYFYNREAKRFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     K"
     gene            12568..>12876
                     /locus_tag="VYN80_001363"
     CDS             12568..>12876
                     /locus_tag="VYN80_001363"
                     /inference="COORDINATES: protein motif:HMM:NF018653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1541263.1"
                     /translation="MSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDIK
                     KGIGLIERVRLGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQ
                     E"
BASE COUNT         3936 a         2174 c         3018 g         3748 t
ORIGIN      
        1 tttaatgaca aaaaatataa atctaacaaa tgaagaatta gagctgatac aaggtggagc
       61 agattcatat ggtaaaaatc ctaatggtag gtacgattgg gaaatagaac cagtattaac
      121 tctgccggtt catggatttt gttccagagg cacctatgat ttaggatata ttggaggagg
      181 caatcatctt tgcaaaggaa gtgctgcgag attttaagta aaatttatta gtaatatgaa
      241 gaaacaagtg gagaaagcag agaatttaat atgaaaaaac gggctattca aattttacta
      301 gcattgtcct taatttttta caaatcaact tggttttgga gagttttcaa tcacctcgca
      361 aagccctatc taccagcaag tcgtgaattt tttcagattc tgcttttgat ggagagcgga
      421 gttcttttct tagcggtcat ctatctactg gtttttgcag gaaagaaaat ttttcatttc
      481 aagtggcagc tgaggtactt catctacctt ttactgggct acatcatttc atatatgtct
      541 gacttcctct tttcgtattt catatccctg tcttcaaatc agatttcttt gaatgaaacg
      601 atagaaatga tggggagaca ggagttccct tatttcttgc tcatcgtttg cttcatcgcc
      661 cctattgctg aggaattgat ttatcgaggt gtgcttatga caaccttttt caaaaactca
      721 ccttggtacg gagatgtttt gctttctgct attattttcg gttatattca tatcaatttt
      781 gctttaactc ctcttgcttt tttcatttat gctagtggag gtctgatttt agctctattg
      841 tatcgcatga ctaaaaatct ctactatcca atactagttc atattctcat caatatcact
      901 gccttctggg atgtttggtt actcctattt tcaggaagtt agcttactaa aaaaatgtcg
      961 gaattttccg gcattttctt ttttcacaaa tagtcaacgt ttttctttcc gatactgaag
     1021 tggtgtgtag ccacttattt ttttgaattg attttgaaaa taagattggc gtgagaaagg
     1081 cagatagtga agatagttaa gaagaatagg atgttctttt ttcctttttg gaaaacttct
     1141 aaaatatggt ataatgaaaa gataaagaag ttgggggtag aagatgaaca ttcaacaatt
     1201 acgctatgtt gtggctattg ccaatagtgg tacttttcgt gaagctgctg aaaagatgta
     1261 tgttagtcag ccgagtctgt ctatttctgt tcgtgatttg gaaaaagagt tgggctttaa
     1321 gattttccgt cggaccagct cagggacttt cttgacccgt cgtgggatgg aattttatga
     1381 aaaagcgcaa gaattggtta aaggatttga tatttttcaa aatcagtatg ccaatcctga
     1441 agaagaaaaa gatgaatttt ccgttgctag ccagcactat gacttcttac caccaactat
     1501 tacggccttt tcagagcgct atcctgacta taagaacttc cgtatttttg aatcaactac
     1561 tgttcaaata ttagatgaag tggcgcaagg gcatagtgag attgggatta tctacctcaa
     1621 caatcaaaat aaaaagggga ttatgcaacg ggttgaaaag ttaggtctgg aggtcatcga
     1681 attgattcct ttccataccc atatttatct ctgtgagggt catcctttag cccagaaaga
     1741 ggaattagtc atggaggatt tagcggattt accaacggtt cgtttcactc aagagaaaga
     1801 cgagtacctt tattattcag agaactttgt cgataccagc gctagctcac agatgtttaa
     1861 tgtgacagac cgtgccacct tgaatggtat tttggagcgg acggacgcct atgcgacagg
     1921 ttctggattt ttagatagtg acagtgttaa tggcattaca gttattcgtc tcaaggataa
     1981 cctagataac cgcatggtct atgttaaacg tgaagaagtg gagcttagtc aagctgggac
     2041 tctcttcgta gaagtcatgc aagaatattt tgatcaaaag aggaaatcat gaaaaaaaga
     2101 gcaatagtgg cagtcattgt actgctttta attgggctgg atcagttggt caaatcttat
     2161 atcgtccagc agattccact gggtgaagtg cgctcctgga tccccaattt cgttagcttg
     2221 acctacctgc aaaatcgagg tgcagccttt tctatcttac aagatcagca gttgttattc
     2281 gctgtcatta ctctggttgt cgtgataggt gccatttggt atttacataa acacatggag
     2341 gactcattct ggatggtctt gggtttgact ctaataatcg cgggtggtct tggaaacttt
     2401 attgacaggg tcagtcaggg ctttgttgtg gatatgttcc accttgactt tatcaacttt
     2461 gcaattttca atgtggcaga tagctatctg acggttggag tgattatttt attgattgca
     2521 atgctaaaag aggaaataaa tggaaattaa aattgaaact ggtggtctgc gtttggataa
     2581 ggctttgtca gatttgtcag aattatcacg tagtctcgcg aatgaacaaa ttaaatcagg
     2641 ccaggtcttg gtcaatggtc aagtcaagaa agctaaatac acagtccaag agggtgatgt
     2701 cgtcacttac catgtgccag aaccagaggt attagagtat gtggctgagg atcttccgct
     2761 agaaatagtc taccaagatg aggatgtggc tgtcgttaac aaacctcagg gaatggttgt
     2821 gcacccgagt gctggtcata ccagtggaac cctagtaaat gccctcatgt atcatattaa
     2881 ggacttgtcg ggtatcaatg gggttctgcg tccagggatt gttcaccgta ttgataagga
     2941 tacgtcaggt cttctcatga ttgctaaaaa cgatgatgcg catctagtac ttgcccaaga
     3001 actcaaagat aaaaagtctc tccgcaaata ttgggcgatt gttcatggaa atctgcctaa
     3061 tgatcgtggt gtaattgaag cgccgattgg ccggagtgaa aaagaccgta agaaacaggc
     3121 tgtaactgct aaagggaagc ctgcagtgac gcgttttcac gtcttggaac gctttggcga
     3181 ttatagctta gtagagttgc aactggagac agggcgcact catcaaatcc gtgtccacat
     3241 ggcttatatc ggccattcag tcgctggtga tgaggtctat ggtcctcgca agactttgaa
     3301 aggacatgga caatttcttc atgccaagac tttaggtttt actcatccga gaacaggtaa
     3361 gaccttggaa tttaaagcag atatcccaga gatttttaag gaaaccttgg agagattgag
     3421 aaagtaagaa tgaaaaagaa attaactagt ttagcacttg taggcgcttt tttaggtttg
     3481 tcatggtatg ggaatgttca ggctcaagaa agttcaggaa ataaaatcca ctttatcaat
     3541 gttcaagaag gtggcagtga tgcgattatt cttgaaagca atggacattt tgccatggtg
     3601 gatacaggag aagattatga tttcccagat ggaagtgatt ctcgctatcc atggagagaa
     3661 ggaattgaaa cgtcttataa gcatgttcta acagaccgtg tctttcgtcg tttgaaggaa
     3721 ttgggtgtcc aaaaacttga ttttattttg gtgacccata cccacagtga tcatattgga
     3781 aatgttgatg aattactgtc tacctatcca gttgaccgag tctatcttaa gaaatatagt
     3841 gatagtcgta ttactaattc tgaacgtcta tgggataatc tgtatggcta tgataaggtt
     3901 ttacagactg ctgcagaaaa aggtgtttca gttattcaaa atatcacaca aggggatgct
     3961 cattttcagt ttggggacat ggatattcag ctctataatt atgaaaatga aactgattca
     4021 tcgggtgaat taaagaaaat ttgggatgac aattccaatt ccttgattag cgtggtgaaa
     4081 gtcaatggca agaaaattta ccttgggggc gatttagata atgttcatgg agcagaagac
     4141 aagtatggtc ctctcattgg aaaagttgat ttgatgaagt ttaatcatca ccatgatacc
     4201 aacaaatcaa ataccaagga tttcattaaa aatttgagtc cgagtttgat tgttcaaact
     4261 tcggatagtc taccttggaa aaatggtgtt gatagtgagt atgttaattg gctcaaagaa
     4321 cgaggaattg agagaatcaa tgcagccagc aaagactatg atgcaacagt ttttgatatt
     4381 cgacaagacg gtttagtaaa catttcaact tcctacaagc cgattccaag ttttcaagct
     4441 ggttggcata agagttcata tggaaactgg tggtatcaag cgcctgattc tacaggagaa
     4501 tatgctgtcg gttggaatga aattaagggt gaatggtatt actttaacca aacgggtatc
     4561 ttattacaga atcaatggaa aaaatggaac aatcgttggt tctatttgac agactctggt
     4621 gttgctgcta aaaattggaa gaaaatcgct ggaatctggt attattttaa caaagaaaac
     4681 cagatggaaa ttggttggat tcaagataaa gagcagtggt attatttgga tgttgatggc
     4741 tctatgaaga caggttggct tcaatataag gggcaatggt attactttgc tccatcaggg
     4801 gaaatgaaaa cgggctgggt aaaagataaa gaagcctggt actatatgga ttctactggt
     4861 atcatgaaga caggtgagat agaagttgct ggacatcatt actatctaga agaatcagga
     4921 gctatgaagc aaggctggct taaaaaggca aatgattggt atttctacaa gacagatggt
     4981 tcacgagcta taggttggat taaagacaag gataaatggt acttcttgaa agaaaatggt
     5041 caattacttg tgaatggtaa gacaccagaa ggctatactg tggattcaag tggtgcctgg
     5101 ttagtggatg ttccgatcga gaaatctgct acaattaaaa ctacaagtca ttcagaaata
     5161 aaagaatcca aagaagtagt gaaaaaggat cttgaaaata aagaaacgag tcaacatgaa
     5221 agtgttacaa attcttcaac tagtcaagat ttgacatcct caacttcaca aagctctgaa
     5281 acgagtgtaa acaaatcgga atcagaacag tagtagaaaa gaaggtttta gggccttctt
     5341 tttcctatca actcttttct atttcctgtt attcatgtta taatggataa atatgaataa
     5401 tcggagtgag actatgaaat acaaacggat tgtctttaag gtgggtactt cttctctgac
     5461 aaatgaggat ggaagtttat cacgtagtaa ggtaaaggat attacccagc agttggctat
     5521 gctgcacgag gctggtcatg agttgatttt ggtgtcttca ggtgccattg cggctggttt
     5581 tggagcctta ggatttaaaa agcgtccgac taaaattgct gataaacagg cttcagcagc
     5641 ggtagggcag ggacttttgt tggaagaata tacgaccaat cttctcttgc gtcaaatcgt
     5701 ttctgcacaa atcttgctga cccaagatga ctttgtggat aagcgtcgtt ataaaaatgc
     5761 ccatcaggct ttgtcggttt tgctcaaccg tggggcaatt cctatcatca atgagaatga
     5821 tagtgtcgtt attgatgagc tcaaggttgg ggacaatgac actctaagtg ctcaagtagc
     5881 ggcgatggtc caagcagacc ttttagtttt cttgacagat gtggacggtc tctatactgg
     5941 aaatcctaat tcagatccaa gagccaaacg cttggagaga atcgagacca tcaatcgtga
     6001 gattattgat atggctggtg gagctggttc gtcaaacgga actgggggta tgttaaccaa
     6061 aatcaaggct gcaactatcg cgacggaatc aggagttcct gtttatatct gctcatcctt
     6121 gaaatcagat tccatgattg aggcggcaga ggagaccgag gatggttctt actttgttgc
     6181 tcaagagaag gggcttcgta cccagaaaca atggcttgcc ttctatgctc agagtcaagg
     6241 ttctatttgg gttgataaag gggctgcgga agctctctct caacatggaa agagtcttct
     6301 cttatctggt atcgttgaag cagaaggagt cttttcttac ggtgatatcg tgacagtatt
     6361 tgataaggaa agtggaaaat cacttggaaa aggacgcgtg caatttggag catctgcttt
     6421 ggaggatatg ttgcgttctc aaaaagccaa gggtgtcttg atttaccgtg acgactggat
     6481 ttccattact cctgaaatcc aactactttt tacagaattt tagaggtaaa ctatggtgag
     6541 tagacaagaa caatttgaac aggtacaggc tgttaaaaaa tcgattaaca cagctagtga
     6601 agaagtgaaa aaccaagcct tgctagccat ggctgatcac ttagtggctg ctactgagga
     6661 aattttagcg gctaatgccc tcgatatggc agcggctaag gggaaaatct cagatgtgat
     6721 gttggatcgt ctttatttgg atgcagatcg tatagaagcg atggcaagag gaattcgtga
     6781 agtggttgcc ttaccagatc caatcggtga agttttagaa acaagtcagc ttgaaaatgg
     6841 tttggttatc acaaaaaaac gtgtagctat gggtgtcatc ggtattatct atgaaagccg
     6901 tccaaatgtg acgtctgatg cggctgcttt gactcttaag agtggaaatg cggttgttct
     6961 tcgtagtggt aaggatgcct atcaaacaac ccatgccatt gtcacagcct tgaagaaggg
     7021 cttggagacg actactattc atccaaatgt gattcaactg gtggaggata ctagccgtga
     7081 aagtagttat gctatgatga aggccaaggg ctatctagac cttctcattc ctcgtggagg
     7141 agctggcttg atcaatgcag tggttgagaa tgcgattgta cctgttatcg agacagggac
     7201 tgggattgtc catgtctatg tggataagga tgcagacgaa gacaaggcgc tgtctatcat
     7261 caacaatgct aaaaccagtc gtccttctgt ttgtaatgcc atggaggttc tgctggttca
     7321 tgaaaacaag gcagcaagca tccttcctcg cttggatcaa atgttggttg cagatcgaaa
     7381 agaagctggg ttggaaccaa ttcaattccg cctagatagc aaagcaagcc agtttgtttc
     7441 aggtcaagct gctcaagcac aagactttga taccgagttt ttagactata ttctagctgt
     7501 taaggttgtg agcagtttag aagaagcggt tgcgcatatt gaatcccaca gtacccatca
     7561 ttcggatgct attgtgacgg aaaatgctga agctgcagca tactttacag atcaagtgga
     7621 ctctgcagcg gtgtatgtta atgcctcaac tcgtttcaca gatggaggac aatttggtct
     7681 tggttgtgaa atggggattt ctactcagaa attgcacgcg cgtggtccaa tgggcttgaa
     7741 agagttgacc agctacaagt atgtggttgc tggtgatggg cagataaggg agtaagagat
     7801 gaagattgga tttatcggtt tggggaatat gggtgctagc ttggcaaaat ctgtcttgca
     7861 gactaggacg tcagatgaga ttctccttgc caatcgtagt caagctaagg tagatgcttt
     7921 cattgcagac tttggtggtc aggcttccag caatgaagaa atgtttgcag aagcagatgt
     7981 gatttttcta ggagttaagc ctgctcagtt ttctgaactg ctttctcaat atcagaccat
     8041 ccttgaaaaa agagaaagtc ttcttttgat ttcgatggca gctggattga ccttagaaaa
     8101 actagcaagt cttatcccaa gccaacaccg aattattcgt atgatgccta atacccctgc
     8161 ttctatcggg caaggagtga ttagttatgc cttgtctcct aattgcaggg ctgaggacag
     8221 tgagatcttt tgtcagcttt tagccaaggc tggtctcttg gttgaattag gagaaagctt
     8281 aatcaatgca gcgacaggtc ttgcaggttg tggaccagcc tttgtctatc tctttattga
     8341 ggccttggca gatgcaggtg ttcagacggg attaccacga gaaacagcct tgaaaatggc
     8401 agcccaaact gtggtaggag ctgggcaatt ggtcctagaa agccagcaac atcctggagt
     8461 tttgaaagat caagtttgta gtccaggcgg ttcgactatt gctggcgtag caagcctaga
     8521 agcgcatgct tttcgaggca cagtcatgga ggcagttcat caagcctata aacgaacaca
     8581 agaactaggt aaataagagg tagttttatc tgcctctttt atggtggttg atactcttcg
     8641 aaaatcaaat tcaaactgcg tcagcttcac cttgccgtac tcaagtacag cctgcggcta
     8701 gcttcctagt ttgctctttg attttcattg agtatgaaat gagaagacaa aaagattgtc
     8761 acaaacccct atttttttga tagaatagaa gtagtaaaaa agaaatgagt tagacatgtc
     8821 aaaaggattt ttagtctctc ttgagggacc agagggagca ggcaagacca gtgttttaga
     8881 ggctctgcta ccaattttaa aggaaaaagg agtagaggtg ttgacgaccc gtgaacctgg
     8941 cggagtcttg attggggaga agattcggga agtgattttg gatccaagtc atactcagat
     9001 ggatgctaaa acagagctac ttctctatat tgccagtcgc agacagcatt tggtggaaaa
     9061 agttcttcca gcccttgaag ctggcaagtt ggtcatcatg gatcgtttta tcgatagttc
     9121 tgttgcctat cagggatttg gtcgtggctt agatattgaa gccattgact ggctcaatca
     9181 gtttgcgaca gatggcctca aacccgattt gacactctat tttgacatcg aggtggaaga
     9241 agggctggct cgtattgctg cgaatagtga ccgcgaggtt aatcgtttgg atttggaagg
     9301 gttggacttg cataaaaaag ttcgtcaagg ctacctttct cttctggata aagagggaaa
     9361 tcgcattgtc aagattgatg ctagtctccc tttggagcaa gttgtggaaa ctaccaaggc
     9421 tgtcttgttt gacggaatgg gcttggccaa atgaaacaag atcaactaaa ggcttggcaa
     9481 ccagctcagt ttgaccgttt tgtccgtatc ttagaacaag accagctcaa tcacgcctat
     9541 ctcttttcag gtttctttgg aagcttggaa atggcgcaat ttttagctaa gagcctcttt
     9601 tgtacggata aagttggcgt cttaccatgt gagaaatgcc gaagttgcaa gctgattgaa
     9661 caggaagagt ttccagatgt caccttgatt aagccagtca atcaggtcat caagacagaa
     9721 cgcattcggg aattggtggg acagttttct caagcaggga ttgaaagcca gcaacaggtc
     9781 tttattatcg agcaagcgga taaaatgcat cccaacgcag ccaattctct gctcaaggtc
     9841 atcgaagaac cccagagtga agtttatatt ttcttcttga ctagcgatga ggaaaagatg
     9901 ttaccgacaa tccgaagtcg gactcagatc ttccacttta aaaagcaaga agaaaaactt
     9961 atcttactct tagaacaaat gggacttgtt aagaaaaaag cgactctttt agctaagttt
    10021 agtcaatcgc gagctgaagc agaaaagttg gctaatcagg caagtttttg gaccttggtc
    10081 gatgaaagtg aacgcctgct gacttggtta gtagctaaga aaaaagaaag ttatctacag
    10141 gttgccaaat tagccaactt ggcagatgat aaggaaaaac aggatcaggt tttacggatt
    10201 cttgaagttc tctgtgggca ggacctcttg caggtaagag taagagtgat tctacaagat
    10261 ttactagaag ctagaaaaat gtggcaagct aatgtcagct ttcaaaatgc catggaatat
    10321 ctggtcttga aagaaatata aactcaaaaa tgaatgataa agaaaggaaa gggctgtttt
    10381 atggacaaaa aagaattatt tgacgcgctg gatgattttt cccaacaatt attggtaacc
    10441 ttagccgatg tggaagccat caagaaaaat ctcaagagct tggtagaaga aaatacagct
    10501 cttcgcttgg aaaatagtaa gttgcgagaa cgcttgggtg aggtggaagc agacgctccc
    10561 gtcaaggcca agcatgttcg cgaaagtgtc cgtcgtattt accgtgatgg atttcacgta
    10621 tgtaatgatt tttatggaca acgtcgagag caggacgaag aatgtatgtt ctgtgatgag
    10681 ttgttataca gggagtaagc atgcaaattc aaaaaagttt taaggggcag tctccctatg
    10741 gcaagctgta tctagtggca acgccgattg gcaatctaga tgatatgact tttcgtgcta
    10801 tccagacctt gaaagaagtg gactggattg ctgctgagga tacgcgcaat acagggcttt
    10861 tgctcaagca ttttgacatt tccaccaagc agatcagttt tcatgagcac aatgccaagg
    10921 aaaaaattcc tgatttgatt ggtttcttga aagcagggca aagtattgct caggtctctg
    10981 atgccggttt gcctagcatt tcagaccctg gtcatgattt ggttaaggca gctattgagg
    11041 aagaaattgc agttgttaca gttccaggtg cctctgcagg aatttccgcc ttgattgcca
    11101 gtggtttagc gccgcagcca catatctttt acggcttttt accgagaaaa tcaggccagc
    11161 aaaagcaatt tttcgactcg aaaaaagact atcctgaaac tcagattttt tatgaatcgc
    11221 cccatcgtgt ggcagataca ttggaaaata tgctagaagt ctacggtgac cgctccgttg
    11281 tcttggtcag ggaattgacc aaaatctatg aagaatacca aagaggtaca atttctgaat
    11341 tgctggaaag cattgctgaa acgtctctca agggtgaatg tcttctgatt gttgaaggtg
    11401 ccagtcagga tgtggaagaa aaaggcgagg aagacttgtt cttagaaatc caagctcgta
    11461 ttcagcaagg tatgaagaaa aatcaagcta ttaaggaagt agctaagatt taccagtgga
    11521 ataagagtca actctacgct gcctaccacg actgggaaga aaaacaataa agggagacag
    11581 gatgtaataa ttctgtctgt ttctgtttaa cttaattagt gatgataata taaagatgta
    11641 tcacttggta tagaagcttt ggtattaagt tttttattaa gcccatacgg aataccgatg
    11701 gttggagcag cagttatagc gttcttagaa ggtataaata gaaaaataag gtcattttaa
    11761 atcaaaggat tgataaatca gaaagaaggt gattttttgc gaacatacga aaataaagaa
    11821 gaactaaaag ctgagataga gaaaacattt gagaaatata ttttagaatt tgataatatt
    11881 ccagaaaatt taaaagataa gagagctgat gaagttgaca gaactccagc agaaaacctt
    11941 gcttatcagg ttggttggac caacttggtt cttaaatggg aagaagatga aagaaagggg
    12001 cttcaagtaa aaacaccatc ggataaattt aaatggaatc aacttggtga attatatcag
    12061 tggttcacag atacctacgc tcatttatct ctgcaagagt tgaaagcaaa attaaatgaa
    12121 aatattcatt ctatctctgc aatgattgat tcgttgagtg aggaagaatt atttgaaccg
    12181 catatgagaa agtgggctga tgaagcgact aaaacagcga cttgggaagt gtataagttt
    12241 attcatgtaa atacggttgc accttttgga actttcagaa ctaaaatcag aaaatggaag
    12301 aagatagtat tataaattat atttttaact ttaaaaaatt tcataaaaat ggttaccaaa
    12361 ggcgatagaa gaaaaactat cgtctttttc tttgcaaatt tttaagaagg gaggtgatct
    12421 tgcatggact ttgaatattt ttataacaga gaagcgaaaa gatttaactt cttaaaagta
    12481 ccggagatat tagttgatag agaagaattt cggggcttat cagcagaagc aattatcctt
    12541 tattccatac ttcttaaata gacaggaatg tcatttaaga ataactggat agacaaggaa
    12601 ggcagagtat ttatctattt tactgtcgaa gaaattatga aaagaagaaa tatctcaaag
    12661 ccaactgcca taaaaacatt agatgagctt gatataaaaa aaggaatagg actgatcgaa
    12721 agagtaaggc ttggacttgg taagccgaac atcatttatg ttaaagactt tatgagtata
    12781 tttcaggtaa aagaaaatga cttacagaag tcaaaaaact taacttcaga agtaaaagat
    12841 tttaacctca gaagtaaaga aaatgaactt caagag
//
LOCUS       DAVLWA010000050        12832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLWA010000050 DAVLWA010000000
VERSION     DAVLWA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.050"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(24..221)
                     /locus_tag="VYN80_001364"
     CDS             complement(24..221)
                     /locus_tag="VYN80_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1541264.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(296..1072)
                     /locus_tag="VYN80_001365"
     CDS             complement(296..1072)
                     /locus_tag="VYN80_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1541265.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGKEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1194..1940
                     /locus_tag="VYN80_001366"
     CDS             1194..1940
                     /locus_tag="VYN80_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1541266.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1953..2933
                     /locus_tag="VYN80_001367"
     CDS             1953..2933
                     /locus_tag="VYN80_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1541267.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3128..3448
                     /locus_tag="VYN80_001368"
     CDS             3128..3448
                     /locus_tag="VYN80_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1541268.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3494..3802
                     /locus_tag="VYN80_001369"
     CDS             3494..3802
                     /locus_tag="VYN80_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541269.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3819..5117
                     /locus_tag="VYN80_001370"
     CDS             3819..5117
                     /locus_tag="VYN80_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1541270.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGTIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5268..7706
                     /locus_tag="VYN80_001371"
     CDS             5268..7706
                     /locus_tag="VYN80_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1541271.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7725..8393
                     /locus_tag="VYN80_001372"
     CDS             7725..8393
                     /locus_tag="VYN80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1541272.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8411..9499
                     /locus_tag="VYN80_001373"
     CDS             8411..9499
                     /locus_tag="VYN80_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1541273.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9954..12455
                     /locus_tag="VYN80_001374"
     CDS             9954..12455
                     /locus_tag="VYN80_001374"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1541274.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
BASE COUNT         4165 a         1974 c         2640 g         4053 t
ORIGIN      
        1 ttctctacaa aaagaagaat agcctatttt actattcttc cgagtgattt caatttcttt
       61 ggggaaatat ggagatactt tttaaatact ttggcaaaat atttatagtc tgaaaatcca
      121 acttggtcag agatagcgct taaaggggag ttgggagatt cagccatctt atctatagct
      181 tgtttcaggc gatattgtaa gatatattca tttagagtca tggggaagtc ttctttgata
      241 accttatata gatgcttgtt atctgcaaat agctagagca tttcaaggaa atgaactaga
      301 aataacaatt aatatggtgg ttcagaaata ccttttgata atcaataaga tcttcaggat
      361 gaagtgcgtt gactccatcc atttcatatt tccgatttaa taaacgatat ttgttttcac
      421 caaattgatg aaaagggagt agttgaactt ggtcgatatt taatgagtta aatagagtag
      481 cgaatttttc tgcatcctct aaactattgt taaaattagg aataactggg attcttaaaa
      541 cgatagtttt attttgagaa aaagcataat gaatgttttt aataatcatt tgattaaaaa
      601 ccccagtcac ttttttatgt tttatagaat tataatgttt taggtctgtg tagataaaat
      661 ccacatattg aattaaatca ataaattttt catgatcaac aaaggcagta gtttcaatgg
      721 cagtgtgtat gtgatgttct ttagctgatt ttaagatggc tttagcaaat tcaaactgag
      781 caaatatttc acctcctgat aaagttaaac ctccaccgga ttcttcgtaa aattctttgt
      841 cttttaatac ctctgtaata atttcttcta cagtcttttc tttaccgact aaggtgaatt
      901 tctctcgttg agcatctttc attttttcag gtttcattct ttgagattca ggattagaac
      961 accatggaca gcgcagagga catcctttta aaaaaacagt tgtacgaata cccgggccgt
     1021 catgaattga aaagtgttga atattaaaaa taattccttt agatatttcc ataagagttc
     1081 ctcctcttca taagtctatc ctatcacaaa aacgaaagaa aaacaattac gaacgaaagg
     1141 ttaatttgtt gttttttgct ttttattttg gtagaataaa acggaggtgt aaaatgaaaa
     1201 gattggaaca aattattaaa ttagtatcag aatatgaaaa gatcgacgtt aatacattat
     1261 cggaaaaatt aaatgtatcg aaagtaacga ttagaaaaga tttagataaa ttagagtcaa
     1321 aaggtttatt acacagagag catggatatg ctgtattaaa tagtggagat gacttaaatg
     1381 tacgtttgtc aattaattat gaaattaaga gaaaaattgt tcaggaagca gtaaaattgg
     1441 tgtcagataa tgaaacaata atgatagaat ctggatcgac ctgtgcttta cttgctgagg
     1501 aaatttgcaa gcaaaaaaga aatgttacga ttgtaacaaa ttcatttttt atagcaaatt
     1561 ttgtgagagc ttatgattca tgtcgtatta ttgttcttgg tggagagttt cagaaggatt
     1621 cacaggttac tgtgggacct ttattaaaag aaatgataca gacttttcat gtgcatcaag
     1681 cttttgttgg gacagatggt tacgataaag agatgggctt tacaggaaaa gatttaatgc
     1741 gcagtgaggt agttcaatat atttcagcag cgtcggataa agtcattgta ctaactgact
     1801 caagtaaatt tgataaaagg ggtacagtaa gaagatttgc tttaagtcaa gtctatgaag
     1861 taataacaga cgaaaaactt tctaaacaaa atatagctac attagaaaat gctgggataa
     1921 tggttaaggt agtttcgtaa gaggttaagt gtatgaatca agataggaat aaactgcttt
     1981 ctaaaattgc ttatctgtat tatattgaaa acttaaatca gtcacaaata gcagcaaaat
     2041 taggaattta tagaacctct attagtagaa tgttaacaga agcaaggaat gcaggaattg
     2101 ttaaaattga aatagagaat tttgatacca atatgtttaa gttggaaaat tatgtaaaag
     2161 aaaaatatgg tctggaaagt ttagaaatta ttccaaatga atttgatgat aatccaacaa
     2221 ttttatctga aagaatttct caagttgcag caggtgtcct taggaatcta attgatgata
     2281 atatgaaaat tggcttttct tgggggaaaa gtttaagtaa tttagtagat ttaattcaca
     2341 gtaaaagtgt ccgaaatgtt cacttctatc ctctagcagg tggtcctagt cacatacacg
     2401 ctaaatacca tgtgaataca ctgatttatg aaatgtctag aaaatttcat ggagagtgta
     2461 catttatgaa tgcaacgatt gtgcaagaaa ataaattgtt agcagatggt attttgcaat
     2521 caagatattt tgaaaatttg aaaaatagtt ggaaagattt agatatagct gtagtcggaa
     2581 ttggtgattt tagcaataaa ggaaagcatc aatggttaga catgcttaca gaggatgact
     2641 ttaaagaatt gaccaaagtt aaaactgtag gagaaatttg ttgtcgattt tttgattcaa
     2701 aaggtaaaga agtctatgaa aacttacagg aaagaacgat agcaatctct ttggaagatt
     2761 taaaaaatat tcctcaaagt ttagctgttg cttacggtga tacgaaagta tcttcgattc
     2821 tttctgtctt gcgtgctaat ttagtaaatc atttgattac agacaaaaat acaattttaa
     2881 aagttttgga agaagatggg gatttgactt ttagagagat tctaggtgag tgaaaatgat
     2941 agactgattc agtttatcgt ttttcttttt agttgattgc acatttgtgc ttatataaac
     3001 aaaaatagtt tatctgttgt ttttggattg acaactttat tatgtagttg tattctatag
     3061 ttacaaaaga aaattttaaa atttcaaatg aaaaaagctt tttacatagt gaaatgagga
     3121 ggaatttatg gaaattattg ttccagatca aattatcatg ggtttaattt tatatgctgg
     3181 cgatgcgaaa caacatattt ataaagcatt agattacata aagaacggta catgtgaacg
     3241 atgtgaagaa gaaatccagt tagctgatgc agccttatta gaagctcata atctacaaac
     3301 aaaattcttg gcacaggaag cgtctggtac aaagacagaa attacagctc tctttgttca
     3361 ttcacaagat catctcatga ctagtatgac ggagattaat ttaatcaaag aaattattag
     3421 tttgagaaaa gaacttcata aaaaataata ctagagtatt atcattgtta ttaacataga
     3481 ggaggaaaac ataatggtga agattggttt gttttgtgca gcaggttttt ctactggtat
     3541 gcttgtaaat aatatgaaaa ttgcagcgca agctagtgga gttgaggcag aaatagaggc
     3601 gttttctcag tctaaattag cggattatgc gccaaatata gatgttgcac tattgggtcc
     3661 acaagttgct tatacattag ataaatcaaa agaaatttgt gataagtgtg atgttccgat
     3721 agctgttatt ccgatgatgg actatggtat gttagatggg aaaaaagtat tagatttggc
     3781 cctatctttg attagtgggt aagaaaagga gatttattat ggcaaagatg gatgttcaga
     3841 aaatcattgc accgatgatg aagtttgtga atatgcgtgg cattatagct ctaaaagatg
     3901 ggatgttagc aattttgcca ttgacagtag ttggtagttt attcttgatt atgggacaat
     3961 tgccgttcga aggattaaat aagagcattg ctagtgtttt tggagctaat tggacagagc
     4021 cgtttatgca agtatattca ggaacttttg ctattatggg tctaatttct tgtttttcaa
     4081 ttgcctattc ttatgctaag aatagcggag tagaggcttt accagctgga gttctatctg
     4141 tatctgcatt ctttattttg ctaagatcat cttatatccc taaacaaggt gaggcaattg
     4201 gggatgctat tagtaaagtt tggtttggag gtcaaggaat tatcggtgct atcattatag
     4261 gtttggtagt aggaagtatt tataccttct ttataaagag aaaaattgtt attaagatgc
     4321 cagaacaagt tccacaagct attgccaaac agtttgaagc aatgattcca gcatttgtaa
     4381 ttttcttact ttctatgatt gtatatattt tagcgaagtc attgactaat ggcggaacat
     4441 tcatagaaat gatttattct gctattcaag ttccattgca aggtttaact ggatctttgt
     4501 atggtgcaat tggaattgca ttctttatat catttttgtg gtggtttggt gttcatggac
     4561 aatcggtagt aaatggagta gtgacagctc tgcttttatc taatcttgat gctaataaag
     4621 ctatgttagc ctctgctaat ctatcattag aaaatggtgc acatattgtt actcaacaat
     4681 ttttagattc atttttaatt ctatcaggtt cagggattac gtttggtctt gtagttgcca
     4741 tgctttttgc agcaaaatca aaacaatacc aagccttagg aaaagttgca gcttttccag
     4801 caatatttaa cgtaaatgag ccagttgtat ttggatttcc gattgtcatg aatccagtta
     4861 tgtttgtacc tttcattctt gttcctgtac ttgcagctgt gatagtatat ggaactattg
     4921 caacaggttt catgcagcca ttctcagggg taacattgcc ttggagtaca ccagctattt
     4981 tatcaggatt tttggtgggt ggatggcaag gagttattac tcagctggtg atattagcga
     5041 tgtctacatt ggtttatttt ccattcttta aagtacagga tcgtttagct taccaaaatg
     5101 aaatcaaaca atcttagagg tatttgtgtg ttactgttaa actcacacat ttgtgctaaa
     5161 aattagagag ttaaaatttt tctagttaaa agcttgaaaa tttctataaa aatcggtatt
     5221 atattttcga aagaaataaa aatattttcg aaagaaaggt ggttacgatg gtaaatacag
     5281 aagtagcaag aacaacaatc aagacagaat attttggcag ccttactgaa aggatgaaca
     5341 aatatcgaga agatgtttta aataaaaaac cttatattga tgctgagaga gcagttctag
     5401 caacacgcgc ctatgaacga tacaaggaac aacctaatgt cctaaaacgt gcatatatgc
     5461 tgaaagaaat tttggaaaat atgactatct atattgaaga agaatctatg attgcgggaa
     5521 atcaagcttc ttccaataaa gatgctccta tttttccgga atatacgcta gaatttgttc
     5581 tcaatgagtt ggatcttttt gaaaagcgtg atggagatgt tttctatatt acagaagaaa
     5641 caaaagaaca acttagaagt attgctccgt tttgggaaaa taataattta cgtgctagag
     5701 ctggtgcctt attacctgaa gaagtgtctg tttatatgga aacaggattc ttcggtatgg
     5761 aaggtaagat gaattctgga gatgctcacc tagcagttaa ctatcagaaa cttttgcaat
     5821 ttggcttaag aggttttgaa gagcgggctc gtaaagcaaa agcagctcta gatttaacag
     5881 atccagcaag tattgataaa tatcattttt acgactctat atttatcgta atcgatgcta
     5941 ttaaagtata tgcaaagcgc tttgttgctc ttgctaaaag tttagccgaa aatgcaaatc
     6001 ctaaacgtaa gaaagaatta cttgagattg cagatatttg ctctagagtc ccatatgaac
     6061 cggcaactac ttttgcagaa gctattcaat cagtttggtt tattcaatgt attttacaaa
     6121 ttgaatctaa tggccactct ctttcatatg gccgttttga tcaatatatg tatccatata
     6181 tgaaggctga tttagaaagt ggtaaagaaa cagaagatag cattgttgaa cgtctgacaa
     6241 atctttggat taagacaatt acaattaata aggttcgcag tcaatcacat acattttctt
     6301 cagcaggaag tcctttatat caaaatgtta caattggtgg acagactcga gataagaagg
     6361 atgctgttaa cccattatct tatttggtat taaaatcagt tgcacaaacc catctaccgc
     6421 aacctaatct aactgtacgt taccatgcag gtttagatgc tcgtttcatg aatgagtgta
     6481 ttgaagtgat gaaacttggt tttggtatgc ctgcatttaa taatgatgag attattattc
     6541 cttcttttat tgcaaaagga gtattggaag atgatgctta tgattacagt gccattggat
     6601 gtgttgaaac ggcagttcca gggaaatggg gctatcgttg cacaggtatg agttatatga
     6661 acttccctaa ggttctactt atcacgatga atgatggaat tgatccggct tcgggtaaac
     6721 ggtttgcacc aagctttggt cattttaagg atatgaagaa cttttctgaa ttagaaaatg
     6781 cttgggataa aacactaaga tatttgacac gaatgagtgt tattgttgaa aattctattg
     6841 atttatcatt ggaacgagaa gttcctgata ttctatgttc agcattgact gatgattgta
     6901 ttggtcgtgg aaaacacctt aaagaaggtg gagcagtata tgattatata tcaggattgc
     6961 aagttggaat tgcaaatttg tcggattcat tagctgcaat taaaaaattg gtgtttgagg
     7021 aagaacgtat aagcccaagt cagctttggc atgcactgga aacagattat gccggagaag
     7081 aaggtaaggt cattcaagaa atgttgattc atgatgcacc taagtatggt aatgatgatg
     7141 attatgctga caaattggtt actgctgctt atgacattta tgttgatgaa attgctaaat
     7201 atcctaatac acgttatgga agaggtccaa ttggaggaat tcgttattca ggaacatctt
     7261 ctatctcagc caacgtaggg cagggacgtg gaacattagc aactccagat ggacgcaacg
     7321 cgggtacacc gttagcagag ggttgttcac catcacataa tatggatcaa cacggcccta
     7381 catctgtttt aaaatctgtt tcaaaattac caacagatga aatcgtaggt ggggttctct
     7441 taaatcagaa agtaaatcct caaacgttag ccaaagaaga agataaatta aaactaattg
     7501 ctttgttacg aacattcttt aatcgtttac atgggtacca tattcaatac aatgttgttt
     7561 ccagagagac gctgattgac gctcagaaac atcctgaaaa acacagagac ttaattgttc
     7621 gtgttgcagg atactctgca ttcttcaatg ttctttctaa ggcaacccaa gatgacatta
     7681 taggacgtac tgagcatact ttgtaaaata aagaggttct ttttatggaa tttatgcttg
     7741 acacattaaa tttagatgag attaaaaagt ggtctgaaat tttgccgcta gctggggtaa
     7801 cttcaaatcc cactattgca aaaagagagg gttctattaa tttttttgaa cgaatcaaag
     7861 atgtaagaga attgattggc tctacaccct ctattcatgt tcaggtgatt tctcaagatt
     7921 ttgaaggcat cttaaaggat gctcataaaa ttcgaagaca agcaggagat gatatattta
     7981 tcaaagtacc tgttactcca gctggattac gtgcaataaa ggcgctaaaa aaagagggct
     8041 accatatcac tgcaacagct atttatacag ttattcaggg attattagct atcgaagcag
     8101 gagcggatta cctagctcca tattataata gaatggaaaa tctgaacatt gattcaaatt
     8161 ctgtcattcg tcaattagct cttgctattg atagacagaa ctctcctagt aagattttag
     8221 ctgcatcctt taaaaatgta gcacaagtaa ataatgcttt agctgcaggt gcgcatgctg
     8281 ttacagcagg agcggatgtt tttgaatcag ctttcgccat gccatctatc caaaaggcgg
     8341 ttgatgattt ttctgacgat tggtttgtta ttcaaaatag tcgttccatt tagatagaga
     8401 ggaaatacat atgagaattt ttgctagtcc ttctagatat attcaggggg aaaatgcctt
     8461 gtttgaaaat gccaaatcaa ttttggattt gggaaattgc cctattctat tatgcgatca
     8521 gttggtttat gatattgttg gaaaacgatt tgaagattac ctacataggt atggtttcca
     8581 tattgttctg gcgctattta atggtgaagc ttctgacaat gaaatcaatc gagttgttgc
     8641 cttggctgag aaagaaaatt gtgatagtat tatcggtctt ggtgggggaa agacgattga
     8701 tagcgcaaaa gctattgcag atttgattga aaagcctgtt attattgctc caacaattgc
     8761 atcgaccgac gcacctgtat ctgctttatc tgttatttat acagatgaag gtgcatttga
     8821 tcattatcta ttttattcta aaaatccaga tttagttttg gttgatacaa aagttatttc
     8881 acaagcccct aagcgtttat tagcgtctgg tattgcagat ggtttagcaa cttgggttga
     8941 ggcgcgtgcg gttatgcagg caaatggaaa aactatgttg ggacaacagc aaacattggc
     9001 tggagttgca attgcgaaga aatgtgaaga aacgctgttt gcagatggtt tacaggctat
     9061 ggcagcttgt gaagctaaag tggtgacacc agcattagaa aatattgttg aagctaatac
     9121 tttattgagt ggtctaggtt ttgaaagtgg aggattagct gcggcgcatg caattcataa
     9181 tggttttact gcattgacag gtgacattca tcatttaaca catggtgaaa aagtagctta
     9241 tggaacttta gtacaactat tattggaaaa tagacctaaa gaagaacttg ataagtatat
     9301 tgagttttac aaaaaaattg gtatgccaac aactctaaaa gaaatgcatt tggatcaagt
     9361 tggatatgat gatttaataa aagttggtaa acaagcaact atggagggtg agacaattca
     9421 tcagatgccg tttaagattt cgccttcaga tgttgctcaa gctattatcg ctgtagatgc
     9481 ctatgtaaat tcaaaataaa caataaggac tactgttttc caaatggtag tcttttattg
     9541 atccctgtat tgaattctat agaagattga aataggatga gaacaaatcg attgggaaag
     9601 taaaattaat ttctataaat gttttagcaa ttgtttcgta ctatttcaga ttcagtctac
     9661 tatacgttct tcataaatca aaaagcgaca taggtagtcg gctatttatt gtgagtacat
     9721 taattagcat tccagtttta tcttcggtct aaaataagta ttttgtgcta tacgagataa
     9781 gcttcttgac ttactccttg atttactgca taacaatggg ataaaaagtg ggagatagag
     9841 caattcatag tcatcaaaat taatgagata cagtatacag tttttccttt aaacacattt
     9901 caaattccct caaaaatggt ataatagtaa catcacaaaa ttggagagag accatgagtt
     9961 tttacaatca taaagaaatt gagcctaagt ggcagggcta ctgggcagaa catcatacat
    10021 ttaagacagg aacagatgca tcaaaaccta agttttatgc gcttgatatg ttcccttatc
    10081 cgtctggagc tggtctgcac gtaggacacc cagaaggtta tactgcaacc gatatcctca
    10141 gtcgttacaa acgtgcgcaa ggctacaatg tccttcaccc aatgggttgg gatgcttttg
    10201 gtttgcctgc agagcaatac gctatggata ctggtaatga cccagcagaa tttacagcgg
    10261 aaaacattgc caacttcaaa cgtcaaatca atgcgcttgg attttcttat gactgggatc
    10321 gtgaagtcaa tacaacagat ccaaactact acaagtggac tcaatggatt ttcaccaagc
    10381 tttacgaaaa aggcttggcc tatgaagctg aagtgccagt aaactgggtt gaggaattgg
    10441 gaactgccat tgccaatgaa gaagtgcttc ctgacggaac ttctgagcgt ggaggctatc
    10501 cagttgtccg caaaccaatg cgccaatgga tgctcaaaat cacggcttac gcagagcgct
    10561 tgctcaatga cttagatgaa ctagattggt cagagtctat caaggatatg caacgcaact
    10621 ggattggtaa atcaactggt gccaatgtaa ctttcaaagt aaaaggaaca gacaaggaat
    10681 ttacagtctt tactactcgt ccggacacac ttttcggtgc gactttcact gtcttggctc
    10741 ctgaacatga attagtagac gctatcacaa gttcagagca agcagaagct gtagcagact
    10801 ataaacacca agccagcctt aagtctgact tggctcgtac agaccttgct aaggaaaaaa
    10861 ctggtgtttg gacaggtgcc tatgccatca accctgtcaa tggtaaggaa atgccaatct
    10921 ggattgcaga ctatgtcctt gctagttatg gaacaggtgc ggttatggct gtgcctgccc
    10981 acgaccaacg tgactgggaa tttgccaaac aatttgacct tccaatcgtc gaagtacttg
    11041 aaggtggaga tgtcaaagaa gctgcctaca cagaggatgg cctgcatgtc aattcagact
    11101 tcctagatga actcaacaaa gaagacgcta ttgccaagat tgtggcttgg ttggaagaaa
    11161 aaggctgtgg tcaggagaag gttacctacc gtctccgcga ctggctcttt agccgtcaac
    11221 gttactgggg tgagccaatt ccaatcattc attgggaaga tggaacttca acagctgttc
    11281 ctgaaactga attgccgctt gtcttgcctg taaccaagga tatccgtcct tcaggtactg
    11341 gtgaaagtcc actagctaac ttgacagatt ggcttgaagt gactcgtgaa gatggtgtca
    11401 aaggtcgtcg tgaaaccaac actatgccac aatgggctgg ttcaagctgg tactacctcc
    11461 gctatattga tccacacaat actgagaaat tggctgatga ggacctcctc aaacaatggt
    11521 tgccagtaga tatctacgtg ggtggtgcgg aacatgctgt acttcacttg ctttacgctc
    11581 gtttctggca caaattcctc tatgacctcg gtgttgttcc gactaaggaa ccattccaaa
    11641 aactctttaa tcaagggatg attttgggaa caagctaccg tgaccaccgt ggtgctcttg
    11701 tggcaaccga caaggttgaa aaacgtgatg gttccttctt tcatgtagaa acaggggaag
    11761 agttggagca agcgccagcc aagatgtcta aatcgctcaa gaacgttgtt aacccagacg
    11821 atgtggtgga acaatacggt gccgataccc ttcgtgttta tgaaatgttt atgggaccac
    11881 tcgatgcttc gattgcttgg tcagaagaag gtttggaagg aagccgtaag ttccttgacc
    11941 gagtttaccg tttgattaca agtaaagaaa tccttgcgga aaacaatggt gctcttgaca
    12001 aggtttacaa cgaaactgtt aaagctgtca cagagcaaat tgattccctc aaattcaaca
    12061 cagccattgc ccaacttatg gtctttgtca atgcggctaa caaggaagac aagctctatg
    12121 ttgactatgc caaaggattt atccaattga ttgccccatt tgcacctcac ttggcagaag
    12181 aactctggca aactgttgct gcaacaggtg agtcaatctc ttacgtgact tggccaacat
    12241 gggacgaaag taaactggtt gaagacgaaa tcgaaatcgt tgtccaaatc aaaggtaaag
    12301 tccgtgccaa actcatggtc gctaaagacc tatcacgtga agaattgcaa gaaattgctc
    12361 tagcggatga aaaagtcaaa gcagaaattg acggtaagga aatcgtgaaa gtgattagtg
    12421 taccaaataa attggttaat atcgtcgtta aataacgagt ttattagctc tatctgccac
    12481 cttcaatagt ccactggact attgaagcca actaaattag ttaacattgt tgtgaaataa
    12541 aaagaagtaa ctctagagta tgaactttag agttactttt agaataggtc agaatgaata
    12601 gattagtgaa cgatagcagg aagtgtgaaa ttttattatt taagatttct attattgtag
    12661 tgtcagttat agtattagta atgtcaatag tgggtgcata ttattttaaa aattgtcttt
    12721 gggacaagga aagtttaaat ttgacactca ctttagacac tctctttgca gtttttatta
    12781 ctttctttag tttttcaaat gataaatcaa gcaatatttt tggaatatct cc
//
LOCUS       DAVLWA010000051        12819 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLWA010000051 DAVLWA010000000
VERSION     DAVLWA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.051"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..378
                     /locus_tag="VYN80_001375"
     CDS             <1..378
                     /locus_tag="VYN80_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1541275.1"
                     /translation="LLVLTDRRSRHQIIRLIPNKTAESVNQALTLLLGEHRILSITAD
                     NGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRRWLPKGTKKTTPKEVAFIE
                     NWINNYPKKCLDYKSPSEFLLGG"
     gene            complement(1505..2278)
                     /locus_tag="VYN80_001376"
     CDS             complement(1505..2278)
                     /locus_tag="VYN80_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1541276.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            2454..3857
                     /locus_tag="VYN80_001377"
     CDS             2454..3857
                     /locus_tag="VYN80_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1541277.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            3972..4610
                     /locus_tag="VYN80_001378"
     CDS             3972..4610
                     /locus_tag="VYN80_001378"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1541278.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            4622..5062
                     /locus_tag="VYN80_001379"
     CDS             4622..5062
                     /locus_tag="VYN80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1541279.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            5055..5486
                     /locus_tag="VYN80_001380"
     CDS             5055..5486
                     /locus_tag="VYN80_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1541280.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            5477..5947
                     /locus_tag="VYN80_001381"
     CDS             5477..5947
                     /locus_tag="VYN80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541281.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            5972..6745
                     /locus_tag="VYN80_001382"
     CDS             5972..6745
                     /locus_tag="VYN80_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1541282.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            6742..7542
                     /locus_tag="VYN80_001383"
     CDS             6742..7542
                     /locus_tag="VYN80_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1541283.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            7601..9895
                     /locus_tag="VYN80_001384"
     CDS             7601..9895
                     /locus_tag="VYN80_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1541284.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            9905..>12819
                     /locus_tag="VYN80_001385"
     CDS             9905..>12819
                     /locus_tag="VYN80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1541285.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDA"
BASE COUNT         4307 a         1821 c         2655 g         4036 t
ORIGIN      
        1 ctgttagtct taaccgaccg gcggagcaga caccaaatta taaggttaat tccaaataaa
       61 actgctgaat ctgtcaatca ggcgcttacg ttactattag gggagcatcg tattctgtcc
      121 attactgcag ataatggttc ggagttcaaa cgattgtctg aggtatttcc tgaggaacat
      181 atctactacg cacatgctta ctcttcatgg gagagaggtt caaatgaaaa tcataatcga
      241 ttaattcgga gatggttacc taaaggaacc aagaaaacga ctccgaaaga agtagctttt
      301 atcgaaaatt ggattaacaa ctaccctaaa aaatgcttgg actacaagtc gccaagtgaa
      361 tttcttttgg gtggctaact tcaacttgaa atttgggata gacctttcag aactaaggga
      421 acctgaaaaa ataaataaaa ctcttgaaat atctgggctt atatgctaga atgtagttag
      481 tgtataaggt tcattagaac accaaagccc ctaactagtg ccatagttaa gggctttttt
      541 gacgtatctt agcgatttaa cgggtgttta taggctagtc agtcggtcta cttcttacca
      601 tctagccatt tgcagacgaa atacaagata attccagcta taacatctgc tataatagta
      661 agagcaagct ctggtataag gctcattagt ttcacctcct ctcacgaacc cataggaacg
      721 taatcggtaa ccgatgacaa aaatagtata ccacactaaa tttagatcat caacctcact
      781 taatgcttga actattgtag attatatgct aaaatgtagt taattgataa ggttcattag
      841 aacaccaaag cccctaacta tggccgtagt taagggcttt tttgacgtat cttagagatt
      901 taacgggtgt ttataggcta gtcagtcggt ctactcctta ctatctagcc atctgcagac
      961 gaaatacaag ataattccag ctataacatc cgctataata gtaagagcga gctctatgat
     1021 aaggctcatt agtttcacct cctctcacga acccatagga acgtaatcgg taaccgatga
     1081 caaaaatagt ataccacaat acatttagat catcaaggtc acttaattct tgaaatatag
     1141 ggtctaggcg acaaagtctt taaaatcaga aaaacgcata atatcaggtg tgcaaaaact
     1201 tgatactatg cgttttattg tgggaaggtt tactccattt tctcctgaaa ttgagttttt
     1261 gtccagcctc tgtttttggg gttgctaaga aaataatgtc atgtggtgaa tatttgtaaa
     1321 tcagtcagca gacagaacga tactcttcga aaatctcttc acatcatgtc agcttcgtct
     1381 ttccgtatat atgtgactga cttcatcagt tctatctaca acctcaaaac agtgtttcga
     1441 gctgacttga tcaattttca aatctgtact gtgagcaagc tgagactagc ttcctatttg
     1501 attttcattg aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt
     1561 tttgatctgg cacggtgtct gaaataaagg ttgtgtattt ggagagggga ttaattttaa
     1621 aaaatccagt cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt
     1681 caataatatt cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat
     1741 caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt
     1801 ccttatcgta attgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga
     1861 ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa
     1921 aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca gtcgtactgg
     1981 aatccagata aacacacata cctgaccgaa taaagtcttt agcgagacta gcgattagtc
     2041 ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt gtgttcatag
     2101 aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta
     2161 aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa
     2221 ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct tttatcataa
     2281 gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat
     2341 ttttatgaat attgggttaa catttgaaca ttatccaagt aagcgttcac atattgaaaa
     2401 aataaaatgt ggggattata ataaacttaa tcaaggacga agagagaaga aaaatggaag
     2461 cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg
     2521 aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag
     2581 ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg
     2641 ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac
     2701 tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat gaaagaacaa
     2761 agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag
     2821 gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc
     2881 ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct
     2941 tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta
     3001 ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca tctttacttc
     3061 ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg
     3121 atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta
     3181 agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta
     3241 cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag
     3301 atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag gaactaagac
     3361 gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga
     3421 aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg
     3481 atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag
     3541 atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag
     3601 agttactaga agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg
     3661 ctagagccag ttactttaac caaatgattg ctgatagaac tggtaaagag gttcttacag
     3721 gttcgactga gggtacagct gtggggaata ttgttgtgca gctcatagct atgggacaat
     3781 taaaagggat ggaagaagct caccatgtta ttgaggagtt tctacaatta gagagttatt
     3841 actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttaa
     3901 gttttgtaca agaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga
     3961 ggaggacaag aatgtcagat gtaaaacaag aattaattaa gtatggcaag aagctagtag
     4021 aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat
     4081 tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag
     4141 tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata
     4201 tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa
     4261 cttatgcaac agtattagct tgtctcagag aaccacttcc ggctagtcat tatatgattg
     4321 cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggtacg aaagaattgg
     4381 ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa
     4441 ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt
     4501 gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg
     4561 agatggattt aatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt
     4621 aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt taatggaaat
     4681 gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa
     4741 taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt
     4801 aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga
     4861 tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct
     4921 taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc
     4981 tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt
     5041 tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt aattttgcta
     5101 gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga
     5161 cattaggttt gatgcctgga gagaatattg tagagtttga acattatttt aaaaatcaag
     5221 taaatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa
     5281 gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag
     5341 ggtttaatat ccctctccta gtggaattaa tatcaagtta tgattcaaaa atcaatttag
     5401 aagaaattgt tcaaaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag
     5461 aggaggaaga ggatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg
     5521 tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga
     5581 tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac cggtaggttt
     5641 aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa
     5701 aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa
     5761 tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt
     5821 aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa
     5881 gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt
     5941 tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac
     6001 attagttggg attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc
     6061 gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat
     6121 tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc
     6181 agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga
     6241 aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct
     6301 tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga
     6361 ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat
     6421 tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa
     6481 ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg
     6541 ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg
     6601 attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc
     6661 ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga
     6721 tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa
     6781 gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta
     6841 ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa agattcagct
     6901 tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt
     6961 attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt
     7021 actggtatta aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg
     7081 ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct
     7141 ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc
     7201 ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga
     7261 acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg
     7321 attccatcaa tggttggtat taattttgga ttagaattta agcaggggga acttgttatt
     7381 tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta
     7441 ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg
     7501 gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg
     7561 tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa
     7621 agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat
     7681 ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg
     7741 aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct
     7801 tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc
     7861 aagagatcaa agccactatg aattacttgg ggaactttac attgagcata tagatattca
     7921 gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt
     7981 tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta cgagttttaa
     8041 taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat
     8101 taatttgggt agaaataaac ggtttaatga tgaagtatct aaactggatt caagtacaat
     8161 tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc
     8221 taaggttacg gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac
     8281 agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc
     8341 tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa
     8401 atatcaggag caatttaata gagttgattt taaactagac tatagtaaag attgtcttag
     8461 cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt
     8521 gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgc
     8581 caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat
     8641 taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga
     8701 atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa
     8761 aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc
     8821 tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac
     8881 tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga
     8941 aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt
     9001 gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg
     9061 aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg
     9121 cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt
     9181 aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag aatggttaga
     9241 agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg ggctttatcc
     9301 ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta aaatcactat
     9361 caataggaga ttatcaaacg ctaatttttt atcttcacag gagagggagc aagcgattaa
     9421 taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct
     9481 gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt
     9541 gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg
     9601 taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt
     9661 atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt
     9721 aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt
     9781 ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt
     9841 acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata
     9901 agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg
     9961 gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac
    10021 gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa
    10081 cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa
    10141 accatatgca gcgattgaac ttcatcctgc aaaagtttgt aaaccaacaa gttgtattcc
    10201 acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct
    10261 aaacattcca gttttcttgg ttattatgtc ggctggggag cgtaatacag ttcctccaga
    10321 gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa
    10381 ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc
    10441 caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat
    10501 gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac
    10561 taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt
    10621 gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca
    10681 ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc
    10741 agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa
    10801 tttcgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa
    10861 aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt
    10921 tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg
    10981 attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata
    11041 tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc
    11101 taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa
    11161 ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag gtaattcctg
    11221 gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta
    11281 tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa
    11341 agaaaatcca aataatttac atattttatt gaataattac aggacagata agacagctat
    11401 gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg aagaaagaag aattagagtt
    11461 agcgaactgg ataagcaaaa attattccat caatcctgta gataatgact ttaggacaac
    11521 aacacttaca ttaaaagggc atactggtca taaacctcag ataaatataa gtggcgataa
    11581 aaatcattat acttatacag aaaattggga tgagaatacc catgtttata ccattacggt
    11641 taatcataat ggaatggtag agatgtctat aaatactgag gggacaggcc cagtctcttt
    11701 cccaacacca gataaattta atgatggtaa tttgaatata gcatatgcaa aaccaacaac
    11761 acaaagttct gtagattaca atggagaccc taatagagct gtggatggta acagaaatgg
    11821 taattttaac tctggttcgg taacacacac tagagcagat aatccctctt ggtgggaagt
    11881 cgatttgaaa aaaatggata aagttgggct tgttaaaatt tataatcgca cagatgctga
    11941 gactcaacgt ctatctaatt ttgatgtgat tctatatgac aataatagaa acgaagttgc
    12001 taagaaacat gttaataatt tgtcgggtga atctgttagt ctagatttca aagaaaaagg
    12061 agcaaggtat attaaagtta aattactaac gagtggagtg cctttgagtt tagcagaagt
    12121 agaggttttt agagaatcag atggtaagca atctgaagag gatatagata aaataacaga
    12181 agataaagta gtctctacaa ataaggtagc tactcaaagt tcaaccaatt atgagggtgt
    12241 agctgcttta gcagttgatg gtaataaaga tggagattac ggacatcatt cggtgactca
    12301 tactaaggca gattctaacg cttggtggca ggtcgatctg ggagaagagt ttacggtttc
    12361 taaagttgat atttataata gaacagatgc cgaacctcag cgtttatcta attttgatgt
    12421 tatttttcta tcttcatcag gagaagaagt ttttagaaga cattttgata aagtagttga
    12481 tggtttgtta tctttaaaag taccttctgt aggggctaag ctagtcaaaa tagaattaaa
    12541 atcagcagct attccgttaa gtttagcgga agttgaagtc tatggttcaa agagaactcc
    12601 gaagaaactt tctaatattg cattaaccaa agaaactcga cagagttcaa cggattacaa
    12661 tggtttttct cgtctagcag ttgatggaaa taaaaacgga gattatggtc atcattcagt
    12721 gactcatacc aaaggagatt ctccttcatg gtgggagata gatttagcac aaaccgaaga
    12781 attagaaaag ttaattattt ataatagaac agatgctga
//
LOCUS       DAVLWA010000052        12742 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLWA010000052 DAVLWA010000000
VERSION     DAVLWA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.052"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(370..2097)
                     /gene="ezrA"
                     /locus_tag="VYN80_001386"
     CDS             complement(370..2097)
                     /gene="ezrA"
                     /locus_tag="VYN80_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1541286.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2179..4125)
                     /gene="gyrB"
                     /locus_tag="VYN80_001387"
     CDS             complement(2179..4125)
                     /gene="gyrB"
                     /locus_tag="VYN80_001387"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1541287.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4140..4703)
                     /locus_tag="VYN80_001388"
     CDS             complement(4140..4703)
                     /locus_tag="VYN80_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1541288.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4788..5342)
                     /locus_tag="VYN80_001389"
     CDS             complement(4788..5342)
                     /locus_tag="VYN80_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1541289.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5353..6576)
                     /locus_tag="VYN80_001390"
     CDS             complement(5353..6576)
                     /locus_tag="VYN80_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1541290.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6613..9063)
                     /locus_tag="VYN80_001391"
     CDS             complement(6613..9063)
                     /locus_tag="VYN80_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1541291.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVGHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELSQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9263..10192)
                     /locus_tag="VYN80_001392"
     CDS             complement(9263..10192)
                     /locus_tag="VYN80_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1541292.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10332..10619
                     /locus_tag="VYN80_001393"
     CDS             10332..10619
                     /locus_tag="VYN80_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1541293.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10653..11987)
                     /gene="ciaH"
                     /locus_tag="VYN80_001394"
     CDS             complement(10653..11987)
                     /gene="ciaH"
                     /locus_tag="VYN80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1541294.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11977..12651)
                     /gene="ciaR"
                     /locus_tag="VYN80_001395"
     CDS             complement(11977..12651)
                     /gene="ciaR"
                     /locus_tag="VYN80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1541295.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3864 a         2727 c         2267 g         3884 t
ORIGIN      
        1 atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca atagacttcg
       61 ttattggacg gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg
      121 aaatcacttt aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt
      181 acttgagtac ggtaaggcga cgctgacgtg atttgaagag attttcgaag agtataaaac
      241 actgttttga ggtggtggat aaatcggctg ggtaaaaact caatctcagg agaaaatgaa
      301 gtaaatctcc ccacaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt
      361 tttcttttat taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt
      421 ggttacacca ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata
      481 atcaaattct ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc
      541 aaatgagcga tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac
      601 aatattataa gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg
      661 ggtaacagat tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt
      721 attgcttgcc gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt
      781 ttccatgtat cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc
      841 attaatatca tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc
      901 aatatctttt agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg
      961 ggttggttct tcttgatttg aagttacctc aacaatagct gcctcaaaac tctctaattc
     1021 tgtctgaata cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa
     1081 acgtgcaata tcttctccca ataaagtatt attctctttc atatgttgaa ggtaagttgg
     1141 aagagttgca agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc
     1201 atacaaggca ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc
     1261 caactgacga atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc
     1321 ttcaatatcc gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggattc
     1381 caaatcctgt aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg
     1441 atccacaata tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc
     1501 ttccacaggg tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt
     1561 ttctaattgt ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac
     1621 tctatgctga agttcctcaa ataaatccaa agcatgaaga acacgaccac tatttttaga
     1681 ttcttgcttc tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cgtcaatcaa
     1741 agtaatttga ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg
     1801 gttatagcct tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa
     1861 atcgacccat ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat
     1921 attttttaca gcttctactt catcatttac tggaagattg tatagttctt cttttctttc
     1981 ttctagcgcc tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc
     2041 cagaactaaa atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc
     2101 aaactccttt tttacttgaa acaatcgtaa cgattatatc atatttttta gaccaaggga
     2161 actacttctc cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa
     2221 ctcacgacga ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc
     2281 agcatcatct acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca
     2341 cagctgatga tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg
     2401 accttcacta taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggctg
     2461 gatatattct ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata
     2521 aacataacca gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt
     2581 acgaatgtgg gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc
     2641 tttcgaaaca tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat
     2701 ttcttcgtta gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg
     2761 aattggaagg atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga
     2821 gtctccttcg acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag
     2881 tttccctgga aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg
     2941 cttggcagcc acacgagcct tggcagctaa aatccctttt tccacgatac gtttggcaat
     3001 ctgtggattt tccatgagga aatcggagaa ggcttcactg aagaggcgat tggtaatctt
     3061 gaccacttcg ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg
     3121 tttaactgag ataactgcag ttaatccttc gcgaacatct tcccctgtta aattatcttc
     3181 attgtctttc agtaacttat tcttacgagc ataatcattg ataacacgag tcagtgccgt
     3241 acggaaacct tgttcatgcg ttccaccttc atgggtatga atattattgg cgaaactcat
     3301 gacattttca tggtaacctg ttgtgtactg catggctacc tcaactgtga tatcatccat
     3361 ctcaccgtct gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc
     3421 aacgtaacta gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttggcg
     3481 cttatctgta attgaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt
     3541 atttaattta tcaaaatcaa agattgttgt ttcagtgaaa atttctgggt ccggtgtgaa
     3601 gtgaaccgtt gttccagttc tatctgtatc cccaaccacc tcaaggtctg caacaacatg
     3661 accacgacgg tattcttggt aatgaatctt accgttttta tggacatgaa cgtctaattg
     3721 agtggaaagg gcattaacta ctgacgaccc caccccgtga agaccacctg aaaccttgta
     3781 tccaccaccg ccgaactttc ctccagcatg aaggactgta aagacggtct caacagcagg
     3841 acggcctgtt ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat
     3901 cgaatcatct ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc
     3961 aattgagtta tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc
     4021 aatgtacatc cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact
     4081 ggcatcataa tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct
     4141 tatatgtcta atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct
     4201 gcagcttgac ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc
     4261 tgatactttt ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag
     4321 ctagaagtca ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa
     4381 acctgatcat ttcgatgaga gaccaaaaaa tgacggccac cttgatttaa aatgtcttcc
     4441 aataggtcag aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc
     4501 ttgtactttt ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta
     4561 gaaaccttta aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat
     4621 gtttcaacaa atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc
     4681 cagatgtaat cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat
     4741 gatttccctg agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta
     4801 ccaaagacat ctcgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc
     4861 tccaccaact cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga
     4921 ccatctacca ctactgtttc cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg
     4981 tcataacaag tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc
     5041 gcacaaatgg ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag
     5101 gtctgattat cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca
     5161 tagtctgata aatctccatc aaagacatga tctgctctta cttggattgc atgcgaaccc
     5221 gttacttgct cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct
     5281 acaactgtca aggcttcaat ttcttcaaag ccctgagcta acataactgc tacttttacc
     5341 atgattttct ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt
     5401 tccgctcttt tcttcagcta aattagtctg gtaactcatc gataaaagca aaccaacacc
     5461 aatcaaatta ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt
     5521 caaaggaagt agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat
     5581 aatcaaacct gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa
     5641 catacggtaa atcaacatga gatagagggc aataaccagg acagagccaa taaagccaaa
     5701 atcttctgca ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga
     5761 agcattaaat ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg
     5821 ctggtaagtc gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattaat
     5881 ttggtaagtc ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat
     5941 agctaagaaa ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca
     6001 agaaacccct gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc
     6061 caagtcactt tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa
     6121 aattaacaaa aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg
     6181 gacaatgaca cgagccaaca tgaggatata ggatatcttc ataaattctg acggttgaaa
     6241 tagggtaatt ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaagcttgg
     6301 attataaaat acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt
     6361 caccttccaa agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa
     6421 ggcaatccag gcgacctgct gccctaaaat gggcagaata ttattgggat aatcatgact
     6481 aacggctata tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa
     6541 actataatcg actctagagt cgagagaacg tttcatatgt actaacctta tactttcata
     6601 caatactatt tatcaaagtt cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg
     6661 agagatggtt ttaacagtcg cttcttctgc tagagatgct actatttgtt tgccgtatcg
     6721 ttttccgatg attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat
     6781 acttcttccc aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa
     6841 ggcatttttc ccttcttgac tcagttcttg attaatcttt ttcgtcaagg gttcttgagg
     6901 attttggaaa ggaagcctcg gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac
     6961 tccctcccag aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt
     7021 ctttagctga tgaacatccc catttttata ctgggccaag tggccaactg taagtaaatc
     7081 cgatactgct agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc
     7141 ttgaatttcc actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt
     7201 taccagaggg aaatctttga ccatgaccac ttcttgttcc tgttttcccc gagattcaat
     7261 cttgacaaat ttagcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat
     7321 ctcaagagta gccgatactc ccaagacttg gcaactctca ggaagtaaag aggacagaag
     7381 agtacggcct gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc
     7441 aagccagtaa tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagt cttctacttc
     7501 caattctgaa aaatactgat ggagattgtc cagagaatct aagatatttt tcctagattt
     7561 gccagactga aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg
     7621 ttgttgaacc ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg
     7681 tatatcgtag gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc
     7741 aataatcagt aaacggtcac tgacaaattc aggattatct tcaagtctgg ttacgagata
     7801 ggcatgatta gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa
     7861 atcttccgtc acaaataagc tctgggatga taaattccca tcatgacgaa ggtctgctag
     7921 aaaatgttgg taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt
     7981 aagccagacc aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg
     8041 caaggaatga taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat
     8101 atctgtatgg aacacttcct tgaggcgttt accttcttct tccatgattt gattttgaag
     8161 aatctttgtc ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa
     8221 gagatagcca taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc
     8281 tttcaatagc aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt
     8341 caacagagaa atatttttag aaaagtcttg agatagtttt cgtggctcca gagaagcttc
     8401 cgttttctta aaatatagac cttgaacttg gaccaagtct ggagaactca ggatagattg
     8461 gttgcgataa gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc
     8521 cagcaagcgt tccaagagac ctttaggaag ctgggtcatc tttttccgta aaaaaagaag
     8581 taattctgct gtagcttggg catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc
     8641 taattctcga cacaaaatcg gcaaactata tttttccagt tcagggaaaa agacctgggc
     8701 caattcgacc gtatcaacac gagggtttct tagctcatag ccttcaaaaa ataaattttc
     8761 cgccaagaga ttagcatcaa actgaacatt atgggctaca aaaatcccat cctccaccaa
     8821 gtcaaatatt tttctggcaa cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt
     8881 caatcctgtc agttctttga tatgagcatc caagggttca tgtggattga catccgtcgt
     8941 atagtgatcg acgatttctc cgtcctcaat cacgacaatt cccacttgga taattttagc
     9001 cttactacct gtgctagttg cctctaaatc caccacaaca tagcgattac caatcgtttt
     9061 cattataaaa aattatacca aaaaaagggc catttggcac ctgcaaacat aggataattt
     9121 tcaaactcaa gaacactact tttcttaaaa atcccatcga atagagcatt ctagcctgct
     9181 gatttttgta aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt tgcatcttcc
     9241 ttctctcaac gaatcatgta ttttatctaa ccatgtcagg atcgtagtca taaaatcctg
     9301 tatcaaggaa acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt
     9361 gagcatgact aaagttgccc tcttgaatca agctacgcgc ttggataaag agttttgcaa
     9421 tctcaacaaa gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat
     9481 gctccaccaa ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg
     9541 tcccatgatt atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga
     9601 agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca
     9661 agagtggacg tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa
     9721 tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag
     9781 cctcagatgc gccagccttt tgtccccaga agttaatctg acgactgcta gttaccatgg
     9841 ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc
     9901 ctgcatcccc aatcgtaata tagtctgtct tgatttcttc caagaagtct aagaaaggct
     9961 tgatacggtc catcatatct tggtgcatga gagcattgac cgcaacgatc aattccttac
    10021 cagcatcatg aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca
    10081 gacgaagacc aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt
    10141 gttcaacttg ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa
    10201 ttataccaca tttctccaaa atcagccatg ctttgaaaat gaaaagggac tttattcttt
    10261 ttgtaacctt tctgtaataa ttctctgccg aaaaaatgat aaaataggta tgtactgtta
    10321 aggagagaat catgcccgta agaaaattac aatcctatga ggtagactat caagaagaat
    10381 taaaccagca gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc
    10441 tcaaggaaat cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta
    10501 gttttatctt tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat
    10561 tcagcttact tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa
    10621 tccaaaatat cgccccaatt ggagcgatat ttttattttt tctttttaga tggtgtctga
    10681 atggcaatct tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt
    10741 ccttttagag catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt
    10801 tgccgggttc tagccttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct
    10861 gtcgaaatac cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag
    10921 ataagaaaat caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg
    10981 gtcatcagtt gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca
    11041 aaacggaaga cacgattatt ttccgaagca atcatctcat agtttgtgaa agttgtatta
    11101 aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag
    11161 ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg
    11221 ctttcgctca catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt
    11281 tgcaaaactg cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc
    11341 ttctgcatac tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt
    11401 aaagacaaaa tccagaaact agccatcacg accacaatca attgctcatg cttttgacta
    11461 gcctgctcca actgactggt attaatcaag acagcagcat acttgatatt ggttgaaacc
    11521 gaactaatat tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta
    11581 aaaacctgaa tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag
    11641 cccaaaaatc gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc
    11701 gtattggaac tgacattagg ttttatttct actttactag cgtccgccct agcattttct
    11761 aaatctttaa tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt
    11821 tcactcagtc catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt
    11881 aaaataatca gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa
    11941 taactaaagt catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata
    12001 cccaacacta cgcaaggttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg
    12061 gacttttgaa acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag
    12121 acggtcaaaa atctgcgtct taggcaaaat cacattttga ttttgaagga aataaactag
    12181 taaatcgaac tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt
    12241 tgataaatta accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg
    12301 tttgagaagg gcctgaattc gcattttaag ttcttctagg tagaaaggtt tggtcagata
    12361 atcatccgct cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat
    12421 aatcagaact ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt
    12481 tttttctggc aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta
    12541 gagaccttct tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa
    12601 tactgaattt gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc
    12661 ctccttatta aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt
    12721 acatgagata gctgagtttt ct
//
LOCUS       DAVLWA010000053        11617 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLWA010000053 DAVLWA010000000
VERSION     DAVLWA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.053"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VYN80_001396"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VYN80_001396"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1541296.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVEYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VYN80_001397"
     CDS             complement(1169..1693)
                     /locus_tag="VYN80_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1541297.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRK
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VYN80_001398"
     CDS             complement(1746..1982)
                     /locus_tag="VYN80_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1541298.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VYN80_001399"
     CDS             complement(2050..2970)
                     /locus_tag="VYN80_001399"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1541299.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEIYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3565
                     /locus_tag="VYN80_001400"
                     /pseudo
     CDS             3160..3565
                     /locus_tag="VYN80_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3781..4362
                     /locus_tag="VYN80_001401"
     CDS             3781..4362
                     /locus_tag="VYN80_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1541300.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4359..5636
                     /locus_tag="VYN80_001402"
     CDS             4359..5636
                     /locus_tag="VYN80_001402"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1541301.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5654..6316
                     /gene="deoC"
                     /locus_tag="VYN80_001403"
     CDS             5654..6316
                     /gene="deoC"
                     /locus_tag="VYN80_001403"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1541302.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSTVKAFETKEAIQNGADEIDMVINVGALKSGDL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVLACQLAQKAGSDFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6303..6692
                     /locus_tag="VYN80_001404"
     CDS             6303..6692
                     /locus_tag="VYN80_001404"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1541303.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6782..7834
                     /locus_tag="VYN80_001405"
     CDS             6782..7834
                     /locus_tag="VYN80_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1541304.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7978..9513
                     /locus_tag="VYN80_001406"
     CDS             7978..9513
                     /locus_tag="VYN80_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541305.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9506..10564
                     /locus_tag="VYN80_001407"
     CDS             9506..10564
                     /locus_tag="VYN80_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541306.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10567..11523
                     /locus_tag="VYN80_001408"
     CDS             10567..11523
                     /locus_tag="VYN80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541307.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3304 a         2074 c         2666 g         3573 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg atgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcaggt tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag aatacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctttctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt taatggcgaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actgatccag acctgatggg caaaggactc tacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa tttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtt aaaggaggtg ttgtttttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagtcaa tggtatataa
     3061 tataagtgat caatttattg atgaaatagg aggtagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg atgtgtacta tgaaaatctt aaaatgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tatggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgctc ttaattgctt tcttactaaa aaaaacgatg
     3361 ggcgaaatgg attgctgctg gtagttattg ctttttagtc ctatggattg cgacagaggg
     3421 ttatttcttc cacatgtcac tagaagattt gatacgactt tggacaagtt tggaaatcct
     3481 gacacaaacg tatcagttgg gcttttatct aaatattttg ttaggaattt tgttgatgat
     3541 taagcatttg aaggttaagc aatagtaatc aaaatgcgcc tgatgataga caataaactt
     3601 tttgttttta tctggactgt gatatttata gttctaaatg gtttattatt tataagtgtt
     3661 gaaagagatt gaagaaaatg gcttcgtgac tcgctttctt cggtcttttt cgttttatta
     3721 tcaatttgta aacgatttac aatttgagtc aaattatggt aaaatagtgt catgagtaaa
     3781 atgtattatg cagaaaatcc tgacgctgct cacgacattc atgagttgag agtagagttg
     3841 ttgggacaaa aaatgacctt tttgacggat gcgggtgttt ttagcaagaa aatagttgac
     3901 tttggaagtc aactcttgct caagtgtctg gatgtcaacc aaggagagac agtcctagat
     3961 gtaggctgtg gttatggtcc attaggtttg tccttggtca aggtttacgg agttcaggcg
     4021 accatggttg atattaacac tcgtgccctg gatttagctc ggagaaatgc tgaaaaaaat
     4081 aatgcaaaag cgacgatatt tcaatctaat atttatgcaa gagttcaagg gcgatttgac
     4141 catgtcattt ccaatcctcc tatccgtgcg ggcaagcaag tcgttcatga aatcatcgag
     4201 aaaagtaaag atttcttgga aactggtggg gatttgacaa tcgtcatcca gaaaaaacaa
     4261 ggggctccaa gtgctaagtc caagatggag gatgtgtttg gtaattgtga aatccttaaa
     4321 aaagacaaag gatactatat ccttagaagt gtgaaagaat gagagcagtt gatttaatcc
     4381 aaaaaaaacg agacggtcaa gaactgactt caagtgaaat tgaatggctg atagaaggct
     4441 atgtgtcagg aactgttcct gattatcaga tgtctgcctt tgctatggct gtttatttta
     4501 aaggaatgac gactcgtgaa atttctgatt taacgatgaa tatggttaag actgggcaag
     4561 agtttgacct gtcagccatt gatggtgtga aagttgacaa gcattctact ggtggtgtcg
     4621 gcgataaggt aaccttgatt ttagctcctc ttgttgctag cttcggtgtg cctgtagcaa
     4681 aaatgagtgg tcgtggtctt ggccatacag gcggaacaat tgataaattg gagtccatta
     4741 agggctatca agtagaacgt agtcaagagg attttattcg tcaggttcag gatattggag
     4801 tatctgtcat tgggcaatca gaccagctgg tcaaagcaga taagcttctc tatgcccttc
     4861 gtgatgtgac cgcaactgtc gacacgattc ctttgattgc gagttcggtg atgagcaaga
     4921 aaattgcggc aggggcggat gctattttgc tagacgtgac tgtcggtgag ggtgccttca
     4981 tgaagacggt tgatgaggcg cgtgagttgg ctcaaactat ggtagatctt ggtaaggttg
     5041 ttggacggaa gacagtagca gtgattacgg atatgagcca gcctttggga cgagcgattg
     5101 gaaatcgtct ggaaatcctt gaagcattgg agattttgca aggtcaagga cgtcaggata
     5161 ttacccactt tatttgtgaa ttggctcaaa ttatgcttgg tttggcaaat gttaacaaaa
     5221 ctgttgaaga agttcgccaa catcttgaga atggtcaagc actggctaag tttgaggaaa
     5281 tggtgcaagc ccaaggtgga gacttggaag acctctattg tcctgtaaat gtagctcatg
     5341 tggtggaaat ccctgctcag gaaacgggtg tcatttcagc tcttccagct atggactttg
     5401 gtctttatgc catgaggctg ggtgctggtc gtgcagttaa gtctgatgcc ttggactatg
     5461 aaacaggaat tgtttttgaa aagaagattg gagactccgt tcaaaagggt gaaattgttg
     5521 caaaagtata cacaaatgaa aaaattccac ctcaactagt tacagatttc caaaaatgtg
     5581 ttaaaataag tgatgaagtg aaaaaaatcc gtgaaattgt agaaatcatc tcctaaatgg
     5641 agggaaaacc gaaatgaaat taaataaata tatagatcat acgcttttaa aacaagatgc
     5701 aaagaaaaaa caaattgata gtttgttgtc tgaggctaga gagtatgact ttgccagtgt
     5761 ttgcgttaat ccgacctggg ttgaacatgc taaaaaagga cttgaaggca cagatgttaa
     5821 ggtttgcaca gtagtaggtt tccccttggg agcaacaact tcaactgtga aagcttttga
     5881 aacaaaagaa gctatccaaa atggggctga tgagattgac atggtaatca atgttggggc
     5941 tctcaagtca ggtgatttgg ccttggttga gtcggatatt cgtgcagtag tggaagcaag
     6001 tggggacaag ttagtgaaag tcattattga agcttgcctt ctgacagacc aagaaaaagt
     6061 tttggcttgc caattagccc aaaaagcagg atctgacttt gtcaaaacat ctactggctt
     6121 ttcaactggt ggtgctacga tagcagatgt tagattaatg cgtgaaacag ttggatctga
     6181 tatgggagtc aaggctgctg gtggagctcg ttcttatgca gatgctcttg cctttgtcga
     6241 agcaggtgcg acccgtatcg gaacgtcagc tggggtagct attttaaaag gagaattggc
     6301 agatggcgac tactgagttg attgaactgg caattgaaac cagcaaacat gcctatgtcc
     6361 cctattctca ctttcctatc ggagcagttt tagtagccaa agacggaagt gtttacacgg
     6421 gagtgaatat cgaaaatgct agctatcctt tgaccaattg cggtgagaga acagctattt
     6481 ttaaggctat atctgaaggc caaagagaat tttcagaatt gattgtctat ggtcaaactg
     6541 aaaaaccgat ctcgccatgt ggtgcttgtc gccaagtgat ggctgaattt tttgaacaag
     6601 atctaaaagt gaccttagtc gcaaaagata aatcgacggt cgagatgacg gtcggggaat
     6661 tacttccata ctcttttaca gacttaaact agtctgagtc gctctctgag tggcacggtc
     6721 cttgtggcca accaatccat acttgcaaca tcgttgcaca tcttatttag gaggttcagt
     6781 aatgaacaag aaacaatggc taggtcttgg cctagttgca gtggcagcag ttggacttgc
     6841 tgcatgtggt aaccgctctt ctcgtaacgc agcttcatct tctgatgtga agacaaaagc
     6901 agcaatcgtc actgatactg gtggtgttga tgacaaatca ttcaaccaat cagcttggga
     6961 aggtttgcag gcttggggta aagaacacaa tctttcaaaa gataacggtt tcacttactt
     7021 ccaatcaaca agtgaagctg actacgctaa caacttgcaa caagcggctg gaagttacaa
     7081 cctaatcttc ggtgttggtt ttgcccttca caatgcagtt gaagaagcag ctaaagaaca
     7141 cactgacttg aactatgtct tgattgatga tgtgattaaa gaccaaaaga atgttgcgag
     7201 cgtaactttc gctgataatg agtcaggtta ccttgcaggt gtggctgcag caaaaacaac
     7261 taagacaaaa caagttggtt ttgtaggtgg tatcgaatct gaagttatct ctcgttttga
     7321 agcaggattc aaggctggtg ttgcgtcagt agacccatct atcaaagtcc aagttgacta
     7381 cgctggttca tttggtgatg cggctaaagg taaaacaatt gcagccgcac aatacgcagc
     7441 cggtgcagat attgtttacc aagtagctgg tggtacaggt gcaggtgtct ttgcagaggc
     7501 aaaatctctc aacgaaagcc gtcctgaaaa tgaaaaagtt tgggttatcg gtgttgatcg
     7561 tgaccaagaa gcagaaggta aatacacttc taaagatggc aaagaatcaa actttgttct
     7621 tgtatctact ttgaaacaag ttggtacaac tgtaaaagat atttctaaca aggcagaaaa
     7681 aggagaattc cctggcggtc aagtgatcgt ttactcattg aaggataaag gggttgactt
     7741 ggcagtaaca aacctttcag aagaaggtaa aaaagctgtc gaagatgcaa aagctaaaat
     7801 ccttgatgga agcgtaaaag ttcctgaaaa ataatggatg gaagtcattt cttaggaagc
     7861 ggccaagggc cgctttttta agagttgaga caaagtcatt ttgtctcagt aaaacttgct
     7921 actagacaaa ctagcaagtt ttattgaaat aaaataagac tctgaaagga agagcacatg
     7981 gcacacgaaa atgtcattga gatgcgtgat attaccaagg tgtttggtgg atttgttgcc
     8041 aacgacaaaa tcaacttgca cctacgaaaa ggtgaaattc atgcactttt aggagaaaat
     8101 ggggctggta agtccacgct aatgaacatg ttagcaggcc ttcttgaacc aactagtggt
     8161 gaaatcgcgg tcaacggtca agttgtcaat ctcgactccc catctaaagc agctagcttg
     8221 ggaatcggga tggttcacca gcactttatg ttggttgaag ccttcacagt ggctgaaaac
     8281 atcattttag gtagtgaatt gactaaaaat ggtgtgctag atatcgctgg agctagcaaa
     8341 gaaatcaagg ctctttctga acgttatggc ttagctgttg acccttctgc caaggtagca
     8401 gatatctcag ttggagccca acaacgtgta gaaattttaa aaacacttta tcggggggct
     8461 gatatcctta tctttgacga accaacggct gttttgactc catcagaaat tgatgagttg
     8521 atggctatta tgaaaaatct tgtcaaagaa ggaaaatcaa ttatcttgat tacccacaaa
     8581 ttggatgaaa ttcgcgcagt ttctgaccgt gttacagtta tccgtcgtgg gaaatcaatt
     8641 gaaaccgttg aaattgcagg ggctaccaat gctgatttgg cggaaatgat ggtaggacgt
     8701 tctgtttcct ttaaaacaga gaagcaagcc tctaaaccaa aagaagtggt tttgtctatc
     8761 aaagatttgg tggtcaatga aaaccgtggt gttccagctg ttaaaaatct gtccttggat
     8821 gttcgtgctg gagagattgt tggtattgcg gggattgatg gaaatggtca gtctgaactg
     8881 attcaagcca ttacaggtct tcgtaaggtt gaatctggta gcattgagct aaaaggagat
     8941 tcaattgtag gcttgcaccc acgtcagatt acagaactaa gtgttgggca cgttccagaa
     9001 gaccgtcacc gtgatggctt gattttggaa atgatgatat ctgaaaatat tgcccttcaa
     9061 acctactata aagaaccaca tagtaaaaat ggaattttga attattcaaa tattacttct
     9121 tatgctaaaa agctgatgga agagtttgat gttcgcgctg ccagtgaatt agttcctgca
     9181 gctgcactct caggaggaaa tcaacaaaaa gcaattattg ctcgtgaaat tgatcgagat
     9241 cctgatctcc ttatcgttag ccagccaact cgtgggttgg atgtcggtgc cattgagtat
     9301 atccacaaac gcttgattga agagcgtgat aatggcaagg ctgtccttgt tgtcagcttt
     9361 gaattggatg agattttaaa cgtctcagac cgtattgccg ttatccacga tggtaagatt
     9421 caaggtattg tatcaccaga aacaaccaat aaacaagaac ttggtgtctt gatggctggt
     9481 ggaaacttgg gaaaggagaa gagtgatgtc taaaaaatta caacaaattt cggttccctt
     9541 gatttctgta tttctaggaa ttttactcgg agccattgtc atgtggatct tcggttatga
     9601 tgctatttgg ggctacgaag aattgttcta tacagccttt ggcagtctgc gtgggattgg
     9661 agaaatcttc cgtgctatgg gtcctctggt cttgattggt cttggttttg cggttgccag
     9721 tcgagctggt ttctttaacg tcggacttcc tggtcaggct ttggcaggtt ggattctcag
     9781 tggttggttt gcgctgtcgc atccagatat gccccgtccc ttgatgattc tagcaaccat
     9841 cgtgattgcc ttgattgctg gtgggattgt cggagcgatt ccaggtattc ttagggccta
     9901 tctagggacg tcagaggtta ttgtaaccat catgatgaac tacattgtct tgtatgtagg
     9961 gaatgccttt atccatgctt tccctaaaga cttcatgcaa agtacagatt cgaccattcg
    10021 tgttggggct aatgcaacct atcagacacc ttggttggct gagttgactg gtaactcacg
    10081 gatgaatatt ggtattttct ttgccatcat tgccgttgca gttatttggt tcatgctcaa
    10141 gaaaacaact cttggttttg aaatccgtgc agttggtctt aatccacatg cttcagaata
    10201 tgctggtatt tctgccaagc ggactattat cctatctatg attatttcag gtgccttggc
    10261 aggtcttggt ggagctgttg aaggtttggg aaccttccag aacgtctatg ttcaaggttc
    10321 gtcattagct atcggattta acggaatggc ggttagtttg cttgcggcca actcaccaat
    10381 tggtatactc tttgcagcct tcctatttgg cgttctccaa gttggggctc ctggtatgaa
    10441 tgcggcgcag gtaccatctg agcttgtcag cattgtaaca gcgtctatta tcttctttgt
    10501 cagtgttcat taccttatcg aacgctttgt caaaccgaaa aaacaagtta aaggaggtaa
    10561 gtaaagatgt ctattataac cttgctcccc ctcttggtgt cttctatgct gatttactca
    10621 gcacctctca tctttacaag tatcggtggt gttttctctg aacgtggtgg tgtggtaaac
    10681 gttggccttg aaggaattat ggttatgggt gccttttctg gagttgtctt taaccttgaa
    10741 tttgcagaac aatttggagc agcaactcca tggctatcct tgcttgtagc aggattggtt
    10801 ggtagtgttt tctctatcat ccacgcagca gcgacggttc atttccgtgc agaccatgtt
    10861 gtcagcggta cggtattgaa cttgatggcg cctgccttgg ctgttttctt agttaaagtt
    10921 ctctataaca aaggacaaac cgacaaccta agtcaaactt ttggacgctt tgatttccca
    10981 gtcttggcaa atatcccagt gattggtgat atcttcttca agtcaactag tctacttggt
    11041 tatctggcga ttgccttctc attccttgct tggtttattc tctttaagac tcaatttggt
    11101 cttcgtctcc gctctgtcgg tgaacatcct caagcagcgg ataccctggg aatcaacgtc
    11161 tacaagatga gatatttagg ggttattatt tcaggttttc taggtggaat tggcggagcg
    11221 atttatgcgc aatcaatctc agttaacttc tcagtgacaa ctattgttgg acctggattt
    11281 atcgcccttg ctgcgatgat ctttgggaaa tggaatccaa tcggtgctat gctttctagt
    11341 ctcttctttg gactttcaca aagtttggct gttatcggtt ctcaattgcc gttcctacaa
    11401 ggagtgccag cggtttatct tcaaattgca ccttatgttt tgacaattct tgtcttagca
    11461 gccttctttg gaaaagcagt cgcaccaaaa gcagatggta tcaactacat caaatcaaaa
    11521 taagcataca aaaaaacgtc agtccaatga ctgaactgca ccccaaaagt tagacagaaa
    11581 aaatctaact tttggggtgt ttttattatg aaattaa
//
LOCUS       DAVLWA010000054        11557 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLWA010000054 DAVLWA010000000
VERSION     DAVLWA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.054"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            102..755
                     /locus_tag="VYN80_001409"
     CDS             102..755
                     /locus_tag="VYN80_001409"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1541308.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            765..1229
                     /locus_tag="VYN80_001410"
     CDS             765..1229
                     /locus_tag="VYN80_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1541309.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1242..2510
                     /locus_tag="VYN80_001411"
     CDS             1242..2510
                     /locus_tag="VYN80_001411"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1541310.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2524..2784
                     /locus_tag="VYN80_001412"
     CDS             2524..2784
                     /locus_tag="VYN80_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541311.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2795..4156
                     /gene="pdrM"
                     /locus_tag="VYN80_001413"
     CDS             2795..4156
                     /gene="pdrM"
                     /locus_tag="VYN80_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1541312.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4310..5278
                     /locus_tag="VYN80_001414"
     CDS             4310..5278
                     /locus_tag="VYN80_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1541313.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5294..6286
                     /locus_tag="VYN80_001415"
     CDS             5294..6286
                     /locus_tag="VYN80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1541314.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6504..7547
                     /locus_tag="VYN80_001416"
     CDS             6504..7547
                     /locus_tag="VYN80_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1541315.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7611..9296
                     /gene="lpdA"
                     /locus_tag="VYN80_001417"
     CDS             7611..9296
                     /gene="lpdA"
                     /locus_tag="VYN80_001417"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1541316.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9360..10349
                     /locus_tag="VYN80_001418"
     CDS             9360..10349
                     /locus_tag="VYN80_001418"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1541317.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10422..11492)
                     /gene="xerS"
                     /locus_tag="VYN80_001419"
     CDS             complement(10422..11492)
                     /gene="xerS"
                     /locus_tag="VYN80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1541318.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKTYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3398 a         2178 c         2613 g         3368 t
ORIGIN      
        1 tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt ccatagccac
       61 tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac tcgtcttggc
      121 atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat cagtttatgg
      181 agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt caggctctct
      241 tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc tatcacggac
      301 caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct ccaccatcct
      361 tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct catgatttga
      421 cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt cctgctggac
      481 aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg attcaggtgg
      541 tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca cgtaagaaga
      601 aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca agtcctttgt
      661 cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca ttcttaaaag
      721 agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct cagttagcga
      781 cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat aagaaaccca
      841 atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga ggagagacgc
      901 cccaggaatg cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc aagccagttc
      961 taaaaggtgt catcactttt cctgaattta caccagattt agactggtac acctatgttt
     1021 ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg ctagaatggg
     1081 ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc tttgttgagt
     1141 ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg gataaattgt
     1201 tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta atcaaaaatg
     1261 gtcgtgtaat ggatcccaag tctggtttgg atcaagtttg tgatgtctta gttcaagatg
     1321 ggaaaattat caaaattgcg tctgagatca cggaagaagg agcagaaacg attgatgcta
     1381 ctggtcttgt ggttgctcct ggcttggtcg atattcatgt tcatttccgt gaacctggtc
     1441 aaacacataa agaagacatt catactggtg ccctagcagc cgctgcaggt ggttttacta
     1501 ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact ttgcaagcag
     1561 ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc attactaaaa
     1621 actttaatgg taaaaacttg actgacttta aggcactctt agaagctggt gcggttggtt
     1681 tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc atggaggaag
     1741 ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg aacggtgttc
     1801 ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct actggggtgg
     1861 ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa gcccatgttc
     1921 acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct caggggttag
     1981 gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa gcacttcttt
     2041 taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca gaccgtcgtg
     2101 ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac cacgcgcctc
     2161 atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt atgactggct
     2221 tagaaacatc cctgtctctc agcttgacct atttagtaga agctggtgag ttgagcttga
     2281 tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt gaagcaggtt
     2341 acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct gaccgctttg
     2401 tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa accttaaaag
     2461 ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga taggagttag
     2521 aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag tggaatttac
     2581 tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc tgaccatgcg
     2641 gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg tgcgtgttgg
     2701 catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg tctgaaactc
     2761 tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt gtttacgaga
     2821 gaagttagta ttatttttaa aaattttctt cccaatcctg atctaccaat ttgccaatta
     2881 ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg acttggctgg
     2941 tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag ggattgtgtc
     3001 agccttggtg cctatcattg gtcaccatct tggtcgaggc aaaaaggaag aagttgcgtc
     3061 tgatttttac caatttattt atttggcctt gggcctatct gtggtcttgc tggggatggt
     3121 acttttcttg gcaccaataa tcttgaatca tattgggtta gaagcagcag tagcggcagt
     3181 agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc tctttagcgt
     3241 cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc tcatgctttt
     3301 gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct ttggtgttcc
     3361 agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg tcttgcttgg
     3421 gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc ttgagaaacg
     3481 aattccactt aatatggata acattaagga aggagttcgt ttaggtctgc ctattggggg
     3541 aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg ctaagttttc
     3601 gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta tgtacgcctt
     3661 tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg gagccaagcg
     3721 atttgatgat gcgaaaacct atattggtct aggaagatgg actgctctca tttttgcggc
     3781 cttcacccta accttccttt acatttttag gggaaatgtg gccagtcttt atggtaacga
     3841 cccaaaattt atcgatttga cagcgcgttt tttaacttat agtcttttct tccagttagc
     3901 agataccttt gcggcaccgc ttcagggaat tttgcggggg tataaggata cagttattcc
     3961 tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta cgctatttga
     4021 ttccctaaca gattttggag cttattctta ctggatcggc ttgattatta gtttgattgt
     4081 gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg aatctttagc
     4141 aaaatccaaa cgctaaaaag tttgtgaaaa aatattcttg acaagaaaat cccttgtttt
     4201 cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga cagtatttgc
     4261 caaaagcggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga tgtcaacttt
     4321 agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc gcatggactt
     4381 aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact tttctgttgg
     4441 tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc tgattacctc
     4501 aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg gaatgatggc
     4561 tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta tgcatatcgc
     4621 tgaccttgat gctgggaacc ttggtgccaa tggtatcgta ggtggtggta tggggatcgc
     4681 tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg tctgcttctt
     4741 tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg cttctatctg
     4801 gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg cggatatcaa
     4861 gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa ttcctggaat
     4921 gttcatcgaa gatggtaaca atgtcatcga tgtctatgaa ggatttcaga aagctgtgga
     4981 ccatgttcgc agtggcaatg gttcagtctt gattgaaagt gtaacttatc gctggctcgg
     5041 tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat tgtggaaaca
     5101 aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg caagtgccga
     5161 agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg ttaaatttgc
     5221 agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg cagactaagg
     5281 agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta tccttgctat
     5341 gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagatg tcggtgtctt
     5401 cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag aacgtgtccg
     5461 tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag ccatgacagg
     5521 acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca tggacaatat
     5581 cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg ttccaatgac
     5641 tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac aatctctaga
     5701 gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac ctgcggacat
     5761 gaaaggcctg cttaagtctt ctatccgtga taacaaccca gttattatac ttgagtacaa
     5821 gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa ttccacttgg
     5881 ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg gaaaaatgct
     5941 tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa gaaatttcag ttgaaattgt
     6001 tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag tgaagaagac
     6061 tggtaaggtt attctggtca acgatgccca caaaacaagt ggctatatcg gtgaaatttc
     6121 agctattatt tcagaatcag aagcatttga ctatctagat gcaccaatac gccgttgttc
     6181 aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga ttccaacagt
     6241 tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gactagagtt acataatata
     6301 agttatgtaa ctaaaataaa gctgacgaga aactttgtat cttttaggtg cagagctctt
     6361 cgtcattaat atcttatatt cgagcacggg ctaaaagctc ggaaaaaaga taaatctcct
     6421 tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac gccctttgta
     6481 tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact cctgcagcta
     6541 gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca ggtgcaaacg
     6601 gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt cgcatttcgc
     6661 cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc caaggaaccg
     6721 gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa aatatcgaaa
     6781 acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct gataacgtaa
     6841 caccatatgg tgaaatcgag cgtattccaa tgacaccaat gcgtaaggtt attgcccaac
     6901 gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa gttgatatga
     6961 ctgaaatgtt ggctcttcgt aagaaggttc ttgagccaat catggaagca actgggaaga
     7021 agactactgt aacagacctt ctttcgcttg cagttgttaa gactcttatg aaacacccat
     7081 acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac tatgtcaatc
     7141 ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac aatgctgaga
     7201 agatgagtct ttcagaactg gtggtagcct ttaaggatgt tattggccgt accttggatg
     7261 gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat ttgggaatgt
     7321 ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc cttggtgtca
     7381 gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc ccaatcatga
     7441 gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct aagtttatga
     7501 aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag caagagtatt
     7561 catttttaag atatagataa aggaaggaag acatggcctt agaaataatt atgccaaaag
     7621 ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc ggagaatttg
     7681 taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg gaattggaag
     7741 ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact gtccctgtaa
     7801 cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct ggagcagcat
     7861 cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt aagagcgatg
     7921 atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca gccattaaag
     7981 ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt ggaacctgct
     8041 taaaccgtgg atgtattcca accaagacct accttcatag cgctgaaatt attgaaaata
     8101 tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact gttgatatgg
     8161 aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt gttgcaggac
     8221 ttcttcgtag ctacggagtt gctgttcata aaggaattgg tacaatcact aaagacaaga
     8281 acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt gctggtggtt
     8341 caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg actagtgatg
     8401 acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt ggagttgtcg
     8461 gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt atcgaaatga
     8521 tggaccgtat agttccagct atggatgtgg aagtttctaa gaaccttcgc ttgatccttg
     8581 agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt gaggaaaatg
     8641 gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa gctcttcttt
     8701 caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa ttggatcgtg
     8761 gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat gcaccaggtg
     8821 acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa gtttccgctg
     8881 aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct gcagccatct
     8941 acactcttcc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt gagaaatacg
     9001 atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca tctgacgcag
     9061 ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt ggtgtacaca
     9121 ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc gaaatggaaa
     9181 tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct gaagtgatgt
     9241 acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa aaataaaacg
     9301 aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa agggatatca
     9361 tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg gaagaatacg
     9421 cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac aagccatcta
     9481 tcattgttgg tcgtcaccag aacactattg aagaaatcaa ccgtgattat gttcgcgaaa
     9541 atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac gatttaaata
     9601 acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac ttcaagagct
     9661 tctcaactcc ggttatcaat accttggctc aactcggggt taaagctgag ttcacaggtc
     9721 gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc tatatcaacg
     9781 gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc ctcgcaaatg
     9841 cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc cgtgcccgtg
     9901 taaccaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc cgtgatttac
     9961 tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt tcagaagaag
    10021 aattggccga aatcaatcgc atcaaggata ctaagtttgg aacttgggac tggaactatg
    10081 gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt aaggttgaag
    10141 tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt gacttctttg
    10201 gtattgaaga cgttgctgca gtagaagctg tccttcgtgg ggtgaaatac gaacgcgaag
    10261 atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt attagccgtg
    10321 aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac taaccaactt
    10381 ctttttttga tatttattta cataatttaa aatacgtaaa atcataaact atccagagca
    10441 ttcttttgtt catcactaac aatatgggta tagaggtcag tgacttgtgt gctagcatgt
    10501 cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct agttgctagt
    10561 gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta tttagcaacc
    10621 attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag agttaaaaaa
    10681 agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag ataattctct
    10741 aaataagttt tagcaaaagc agcgacattg actgagtcac gtttgccacc ttttcgagta
    10801 acatcaataa ccatcatttt tagattgaga tctcttagat ctagattaac agcttcagat
    10861 aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc tttatttttg
    10921 ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc gatataagtt
    10981 agaaaacctt ctgtttcatc acctagaaag agtttttgct tgatattttc agctctggca
    11041 gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg atagaaataa
    11101 ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact agaaagtgct
    11161 gataaggttc gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt agcattcagc
    11221 aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga catattttcc
    11281 aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga ctccaaaacc
    11341 cagctgaaaa atcggtcata ttccttaagg tattcgtaca gggttgtaaa actgtagggc
    11401 acagccagtt tagattggta gtattccaga acataccagg gcatgagttg ttttagtttg
    11461 tcgattcgtt ccagtaaaat ctcacgtttc atgtattttc tccataatta agtatactag
    11521 tagtatagca tagacttgta tatttttcaa gaaaatt
//
LOCUS       DAVLWA010000055        11515 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLWA010000055 DAVLWA010000000
VERSION     DAVLWA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.055"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            9..158
                     /gene="blpO"
                     /locus_tag="VYN80_001420"
     CDS             9..158
                     /gene="blpO"
                     /locus_tag="VYN80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1541319.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            262..381
                     /locus_tag="VYN80_001421"
     CDS             262..381
                     /locus_tag="VYN80_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1541320.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            849..1220
                     /locus_tag="VYN80_001422"
     CDS             849..1220
                     /locus_tag="VYN80_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541321.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            1849..2232
                     /locus_tag="VYN80_001423"
     CDS             1849..2232
                     /locus_tag="VYN80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1541322.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2284..2973
                     /locus_tag="VYN80_001424"
     CDS             2284..2973
                     /locus_tag="VYN80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1541323.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            3015..3248
                     /locus_tag="VYN80_001425"
     CDS             3015..3248
                     /locus_tag="VYN80_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541324.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3399..4010
                     /locus_tag="VYN80_001426"
     CDS             3399..4010
                     /locus_tag="VYN80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1541325.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4171..4965
                     /locus_tag="VYN80_001427"
     CDS             4171..4965
                     /locus_tag="VYN80_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1541326.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4962..5597
                     /gene="trmB"
                     /locus_tag="VYN80_001428"
     CDS             4962..5597
                     /gene="trmB"
                     /locus_tag="VYN80_001428"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1541327.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5723..6202
                     /gene="rimP"
                     /locus_tag="VYN80_001429"
     CDS             5723..6202
                     /gene="rimP"
                     /locus_tag="VYN80_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1541328.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDGLTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6246..7382
                     /gene="nusA"
                     /locus_tag="VYN80_001430"
     CDS             6246..7382
                     /gene="nusA"
                     /locus_tag="VYN80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1541329.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7404..7697
                     /locus_tag="VYN80_001431"
     CDS             7404..7697
                     /locus_tag="VYN80_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1541330.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7690..7989
                     /locus_tag="VYN80_001432"
     CDS             7690..7989
                     /locus_tag="VYN80_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1541331.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8006..10798
                     /gene="infB"
                     /locus_tag="VYN80_001433"
     CDS             8006..10798
                     /gene="infB"
                     /locus_tag="VYN80_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1541332.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11049..11399
                     /gene="rbfA"
                     /locus_tag="VYN80_001434"
     CDS             11049..11399
                     /gene="rbfA"
                     /locus_tag="VYN80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1541333.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3514 a         2028 c         2580 g         3393 t
ORIGIN      
        1 catactatat gaatacaaaa atgatgtcac aattttctgt tatggataat gaaatgcttg
       61 cttgcgttga aggtggagat attgattggg gaagaaaaat tagttgtgca gcaggggttg
      121 catatggcgc aattgatggg tgtgcaacaa cggtttgaca tttctattgg gaccatttgc
      181 tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggtg tagcctatgc
      241 agcaacatgt tggtggtaat tatggatttt aaaagtttta ttattggttt agtagttggt
      301 atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc ttccaaaaag
      361 gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag cttaatttac
      421 aggggagttt ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc aggatgaaaa
      481 gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt tcatgcttgc
      541 ttttttaatt tttacaattc aagaggtttt gatgaccatt tatgatttga gtgatccaag
      601 gtcaaaataa gtgctatact agcagtgtaa atgttcttgc tcaactaaga taaatcgcac
      661 atttaggagg aaaacttatg aagaaaaaaa tactgattat tttcgttctg tatctgatca
      721 tgtccatctt tctttatccg cttagggaga gtatttggta taatctattt tataccatag
      781 cctatatgat tgcggttatg atctattttt ctttaattaa aaagaaagaa aagaaatgaa
      841 agattttctt gattaaaaag aatcatattc tttgtgagat tgttcctggg tcagttgcct
      901 ttgcttgttt ctacttatct atttctattt cgtcagtttt ccaatttctt ttagtagtta
      961 ttaacttgac ttctattttg gttactgttt atcttactag ggaaatgaga ttaagagagt
     1021 ttgaagatga tgatttggtt agtcctagaa ccaatcaact catgtttatc ggcttgacag
     1081 gctttatgtc tattatttgt ttgtatagag gtatcacagc aggagaatct tatcaacaac
     1141 taatcgctta tattggtgct attctttgct tgatcatcat gcttctactc atttggggtt
     1201 tgaagtatta taaaaagtag gggctaatga agtttttgca atttaggagg tttcgatgac
     1261 aattcaagat ttggatgaaa aattttaaaa aacaatgata tactaaactt gtcaaagttg
     1321 caacgggtgc tgctacatta tgttaaaaat cgctcttgat tgataagttg gatttgttag
     1381 gaaaggagta aataattatg gataagaaaa agatagtttc gactataata tgtatagtat
     1441 ttcttgtagt gtcagtagat aattttttta gagacttaac cccgttactg ttcattttaa
     1501 atattattgg cctgtcttgc ttttcagtgc taacatacat taacataaaa gagatactct
     1561 taaatataag taaatgatga tttggttagt cctagaacca atcaacttat gtttatcggc
     1621 ttggcagatt ttatgtttgt catttgtttg tatagaggta tttcagaaac agaattctat
     1681 caacaactaa tcgcttatat aggtgtattc tctgcttgtt tatcacgctt ctgctcatgt
     1741 ggggcttgaa gtattataaa aagtagcttt ctatcctttt ctgacaggat atttgagtag
     1801 aaggctctat taaattgtta gtgaaataaa tgaatggagg tatttaatat gaaatacaga
     1861 ttattttttg ttattttctt gagtagtgtg ttggatattc ttttagggac atttttacaa
     1921 atctctatcg tatctattgg gtggcttgtt ctttacagtg gtttgtttga agcaggagtt
     1981 ttccttcttg ctaataaagg ggtggcggta aaaatcaagg aagtagatat tcgaaatcgc
     2041 tttaaattta tttttggaaa aaccttatgg tttcaaattc ttttgctcat ctttttgata
     2101 atcaaacttt atcttggttt ggatgcgagg ttgattttat tctatggaca tattttcatt
     2161 gtctttaatg ccttaatgta tctattatct agtagtcagg ttagccttaa aaaaaacaaa
     2221 ctgtcttctt aatcttacag cagtagagcc tgacaaggtg aggtcatagt agggaggatt
     2281 tctatgaaaa agtatcaact tctattcaaa ataagtgcag ttttctctta cttatttttc
     2341 gtatttggtc tttctcagat gacgcttatt attcaaaatt attggcaatt ttcttcccag
     2401 attggcaatt tcgtctggat tcaaaatttc ttgagtttgc tatttagcgg agtcatgatt
     2461 tggattctgg ttaagacagg tcatggttat ctctttcaca ttccaagaaa aaaatggctt
     2521 tggtattcga ttttgacagt attagtggta gtgctccaga tctcttttaa cgttcagaca
     2581 gctaaacatg ttcagtcaac tgctgaaggt tgggctgtat tgatcggtta tagtgggacc
     2641 aactttgctg agctaggtat ctatataact ttgttctttc tgactccact tatggaagag
     2701 ctaatctata gaggattact gcaacacgcc ttttttaagc attcgagatt tggccttgat
     2761 ttgcttcttc cgtcaatttt atttgctctt cctcattttt caagcctgcc tagtttgtta
     2821 gatatcttca tctttgcaac atctggcatc atctttgcta gtttgacccg ctataccaag
     2881 agcatttatc cttcctatgc ggtgcatgtg atcaataata ttttcgcaac attaccattt
     2941 ttgctgactt ttttacatag ggtcttgggg taaaaaaaca aaaggcttgc ttttcagcca
     3001 tagaggagat tatcatgtat aaacacttat ttttcctaga ttccaaaact ttagatcggt
     3061 tgacacccta tattctagtc ttggcttctg acaccattgc ttttaatgtt tttgtgctaa
     3121 cctttgtatc tgcggtggtt tttaatttcc taaattccat gctagcttta atggctatat
     3181 tcataggggc tggctatgtg gtcggatttt ggttactaat actcaatgaa aatcaaagag
     3241 caaactagga aactagccgc aggctgtact tgagtacggc aaggcgacgt tgacgtggtt
     3301 tgaatttgat tttcgaagag tataaaatgg tttgttttgg aaaggctaga actaaagaat
     3361 gacgcgtagg aagtctgttt ggtgaggagg atagttttat ggagtttttt gataaatttc
     3421 atgccttgtg ttttggattt ttagtactaa taattgtcat tacagttcct tatacgatta
     3481 accatggggg tttttttcaa aatgaatctg cattgattct tgtaagtctt cttgtaacct
     3541 cgctgagtgt tgcttatgct agaaagtttg aaatgatttc ttttgggatg ttaagcaaga
     3601 aacaactttt gcttttcatt gcaatctttc ttctaagtgt acttgagacg ctggtttata
     3661 ttcatttctt cgctgtttct tctggctcag gggtccaaca cttggcggaa gtcagcagag
     3721 gaatttccct gtctttgatt ttgactacct cagtttttgg ccccatccag gaggaactca
     3781 ttttcagagg acttcttcaa ggtgcggttt ttgacaattc ttggttaggg cttgtgctaa
     3841 cttcctctct cttttctttc atgcatggac cttctaatgt cccttcgttt attttttatc
     3901 tacttggggg tttgttgctg ggctttgctt ataaaaagag tcaaaaccta tgggtttcta
     3961 ctctagtcca catgttttac aacagttggc cactcctata ttatttataa aaattatgaa
     4021 aaggtaagca gaagatggtg cttacctttt tcaagctata atgataccca agccaatcca
     4081 gaggcttttc tttgagaatc gggttagagt ggttgacgaa taggccaaaa actagtagaa
     4141 tagtaaggaa actttatacg gaggaaagaa atggatttgg gtgataatga gctaacactg
     4201 actcccatac ctgggaaaag tggcaaggct tatatgggta gctatcctga cgggaagcgc
     4261 atctttgtaa aaatgaacac ctctccaatc ctacctggtc tagctagaga acaaattgct
     4321 ccacaattat tatggagtcg tcgtttggca gatgggcgtg atatgtgtgc tcaagaatgg
     4381 ttgacaggca agatattgac cccctatgat atgaatcgta agcaaatcgt caatatttta
     4441 acccgtcttc atcgctcacg tccgttgatg acacaattga gtcgtttggg ctatgccatg
     4501 gaaacacctg tagatttact ccagtcttgg caggaaacgg ctccagatgc tttgcgtaaa
     4561 aatcatttta tcagtgaagt gatggctgat ttacgtcaga ctattccagg atttagagag
     4621 gaccatgcga ccattgtcca tggagatgta cgacatagta attggattga gacagatagt
     4681 ggcttgattt atttagtaga ttgggattcg gttcgcttga ccgatcgcat gtttgatgtg
     4741 gcccatatgc tctgccatta tatttcagaa catcagtgga aggaatggtt gacctactac
     4801 ggttacaagt acaatcaaac ggtattaagt aaattgtatt ggtatggtca attgtcttat
     4861 ttgagtcaga tttccaagta ttatatgaac caagatttag aaaatgtcaa tcgggagatt
     4921 catggtttgc gtcatttccg agacaagtat ggaaagagaa gatgagagtt agaaatcgta
     4981 aaggggcaac agaattacta gaggcaaatc cccagtatgt ggtcctcaat cccttggaag
     5041 ccaaggcaaa atggcgggac ttgtttggca atgataatcc cattcatgtg gaagttggaa
     5101 gtggaaaggg tgcctttgtt tcaggtatgg ccaagcaaaa ccctgacatc aactatatcg
     5161 ggattgatat tcaaaagtct gttttgagct acgctttgga caaggtgctt gaagttggag
     5221 tgcctaacat caagctcttg tgggtagatg gttctgactt aactgactat tttgaagacg
     5281 gtgagattga tcgcttgtat ctgaactttt cagatccatg gccgaaaaaa cgccatgaaa
     5341 agcgtcgttt gacctacaag accttcttgg ataccttcaa acgtatcttg cctgaaaatg
     5401 gagaaattca tttcaagacg gataaccgtg gcttgtttga gtacagttta gtgagctttt
     5461 ctcaatatgg catgaaactc aatggtgtct ggttagattt gcatgccagt gattttgaag
     5521 gcaatgtcat gacagaatac gagcaaaaat tctcaaacaa ggggcaagtt atctaccgag
     5581 ttgaggcaga attttaagag atagcctaaa attaggctgt acaagtgctt ttgctttaca
     5641 taagttggca aacgtgctat actgatagta agaatatgaa aagtgaggcg gggaaatatc
     5701 ttcgcctctt gcttatgagg aggtggacgc aatcgcaaca atcgtagaat tagtcagaga
     5761 agttgtagaa cctgtcatag aagctccttt tgaactcgtg gatatcgagt atggaaagat
     5821 tggcagtgac atgattctca gtatttttgt agataaaccc gaaggaatta ccttgaacga
     5881 cacggcagac ttgacagaaa ttatcagtcc tgtcctagac accatcaagc cagatccctt
     5941 cccagaacaa tatttcctag aaattaccag tccaggtttg gaacgccctt tgaaaaccaa
     6001 ggatgccgtc gctggagcgg ttggaaaata catccatgtc gggctctacc aagccatcga
     6061 taagcaaaag gtctttgaag gaaccttgtt ggccttcgaa gaggacgggt tgactatgga
     6121 atatatggac aagacgcgta agaaaaccgt ccaaattcca tacagtttag tatcaaaagc
     6181 acgtttagca gttaaattat agaaaaagaa aggatagctt ttgaggattc aaaagtgaag
     6241 aaaacatgag taaagaaatg ctagaggcct tccgcatttt ggaagaagac aagggaatca
     6301 aaaaagaaga tatcatcgac gcagtagtag agtcgcttcg ttccgcttat cgcagacgct
     6361 atggtcagtc agacagcgta gctattgact tcaacgaaaa aacaggtgac tttacagttt
     6421 atactgtccg tgaagttgtt gatgaagtat ttgatagccg tttggaaatc agcttgaaag
     6481 atgctcttgc cattaattca gcttatgaac ttggagacaa aatcaagttt gaagaagcac
     6541 cagctgagtt tggtcgtgta gcagcccagt ctgccaaaca aaccatcatg gaaaaaatgc
     6601 gcaagcaaac acgtgccatc acttacaata cttacaaaga acatgagcaa gaaatcatgt
     6661 ctggtacagt agaacgcttt gacaaccgct ttatctatgt caaccttggt agcatcgaag
     6721 cccaattgtc aaaacaagac caaattcctg gagaagtttt tgcttctcat gatcgtatcg
     6781 aagtttatgt ttacaaggtt gaagacaacc ctcgtggtgt gaacgtcttt gttagccgta
     6841 gtcatccaga aatgatcaaa cgtttaatgg agcaagaaat tccagaagtt tatgatggaa
     6901 ctgttgaaat catgagcgtg gctcgtgaag caggtgaccg tacgaaggtt gctgttcgta
     6961 gccacaatcc aaacgtggat gctatcggta caatcgttgg acgtggtggt gctaatatca
     7021 agaagattac tagcaaattc cacccagctc gttacgatgc taaaaatgac cgcatggtac
     7081 caatcgaaga aaatatcgat gttatcgagt gggtagcaga tccagctgaa tttatctaca
     7141 atgccatcgc tcctgctgag gttgaccaag ttatctttga tgaaaacgac agcaaacgtg
     7201 ccttggtggt tgttccagat aacaagcttt ctcttgccat tggtcgtcgt ggacaaaacg
     7261 tgcgcttggc ggctcacttg actggttacc gtatcgatat caagtctgct agcgaatttg
     7321 aagccatgga agacgctgct tcagtagagt tggaagtaga aaacgatact gtagaagaat
     7381 aaaagctgct agaggaggga aagatgaaaa caagaaaaat ccctttgcgc aagtctgttg
     7441 tgtctaacga agtgattgat aagcgtgatt tgctccgcat tgtcaagaac aaggaaggac
     7501 aagtctttat tgatcctacg ggcaaggcca atggccgcgg cgcttatatc aaactagaca
     7561 atgcagaagc cctagaggcg aaaaagaaga aggtctttaa ccgcagcttt agcatggaag
     7621 tggaagaaag cttttatgac gagttgatcg cttatgtgga tcacaaagtg aaaagaagag
     7681 agttgggact tgaataagca aaagataagt aatctcttgg ggcttgctca gcgagcaggg
     7741 cgcatcatat cgggtgaaga attggtggtc aaggccattc aagacggcaa ggccaagttg
     7801 gtctttctag ctcatgatgc tggacccaat ctgaccaaga agattcaaga taaaagtcat
     7861 tattatcaag tagaaattgt aaccgtgttt tcaacactgg aattaagcat agcagtcggg
     7921 aaatcgagaa aggttttggc tgtaacagat gctggattta caaagaaaat gaggtctctt
     7981 atggaataga agaggaggac atgatttgtc taagaaaaga ttgtacgaaa tcgcaaaaga
     8041 acttggaaaa gaaagtaaag aagttgtagc gcgtgcaaaa gagttgggct tggatgtgaa
     8101 aagccactca tcaagtgtgg aagaagctgt cgctgcaaaa attgctgcca gctttaagcc
     8161 tgcagctgct ccgaaagtag aagcaaaacc tgcagcacca aaagtaagtg cagaaaagaa
     8221 agccgaaaaa tctgagccag ctaaaccagc tgtagctaag gaagaggcaa aaccggctga
     8281 gccagttgct ccgaaaacag aaaaagtagc agtgaaaccg caaagtcgta atttcaaggc
     8341 tgagcgtgaa gcccgtgcta aagagcaggc agaacgacgc aagcaaaata agggcaataa
     8401 ccgtgaccaa caacaaaacg gaaaccgtca gaaaaacgac ggccgtaatg gtggaaaaca
     8461 aggtcaaagc aaccgcgaca atcgtcgctt taatgaccaa gctaagaagc agcaaggtca
     8521 gcaaaaacgt agaaatgagc gccgtcagca agaggataaa cgttcaaatc aagcggctcc
     8581 acgtattgac tttaaagccc gtgcagcagc cctaaaagca gagcaaaatg cagagtacgc
     8641 tcgttcaagt gaggaacgct tcaagcagta tcaggctgct aaagaagcct tggctcaagc
     8701 taacaaacgc aaggaaccag aggaaatctt tgaagaagcg gctaagttag ctgaacaagc
     8761 acagcaagtt caagcagtgg ttgaagtcgt ccctgagaaa aaagaacctg cagtggatac
     8821 acgtcgtaaa aaacaagctc gaccagacaa aaatcgtgac gattatgatc atgaagaaga
     8881 tggtcctaga aaacaacaaa agaatcgaag tagtcaaaat caagtgagaa atcaaaagaa
     8941 tagtaactgg aataacaaca aaaagaacaa aaaaggcaat aacaagaaca accgtaatca
     9001 gactccaaaa cctgttacgg agcgtaaatt ccatgaattg ccaacagaat ttgaatatac
     9061 agatggtatg accgttgcgg aaatcgcaaa acgtatcaaa cgtgaaccag ctgaaattgt
     9121 taagaaactt ttcatgatgg gtgtcatggc cacacaaaac caatccttgg atggggaaac
     9181 aattgaactc ctcatggtgg attacggtat cgaagccaaa caaaaggttg aagtggataa
     9241 tgctgacatc gaacgtttct ttgtcgaaga tggttatctc aatgaagatg aattggttga
     9301 gcgtccacca gttgttacta tcatgggaca cgttgaccac ggtaaaacaa cccttttgga
     9361 tactcttcgt aactcacgtg ttgcgacagg tgaagcaggt ggtattactc agcatatcgg
     9421 tgcctaccaa atcgtggaaa atggtaagaa gattaccttc cttgatacac caggacacgc
     9481 ggcctttaca tcaatgcgtg cgcgtggtgc ttctgttacc gatattacga tcttggtcgt
     9541 agcggcagat gacggggtta tgcctcagac tattgaagcc atcaaccact caaaagcagc
     9601 taacgttcca atcatcgtag ccattaacaa gattgataaa ccaggtgcta acccagaacg
     9661 cgttatcggt gaattggcag agcatggtgt gatgtcaacc gcttggggtg gagattctga
     9721 atttgttgaa atctcagcta aattcaacca aaatatcgaa gaattgttgg aaacagtcct
     9781 tcttgtagct gaaatccaag aactcaaggc agacccaaca gtgcgtgcaa tcggtacggt
     9841 tatcgaagcg cgcttggata aaggaaaagg tgcggtcgca acccttcttg tacaacaagg
     9901 taccttgaat gttcaagatc caatcgttgt cggaaatacc ttcggtcgtg tccgtgctat
     9961 gaccaacgac cttggtcgtc gtgttaaagt tgctggacca tcaacaccag tctctatcac
    10021 aggtttgaac gaagcaccga tggcgggtga ccactttgcc gtttacgagg atgaaaaatc
    10081 tgcgcgtgca gcaggtgaag agcgtgccaa acgtgccctc atgaaacaac gtcaagctac
    10141 ccaacgtgtt agccttgaaa acctctttga tacccttaaa gctggggaac tcaaatctgt
    10201 taatgttatc atcaaggctg atgtacaagg ttctgttgaa gccctttctg cctcacttca
    10261 aaagattgac gtggaaggtg tcaaagtgac tatcgtccac tcagcggtcg gtgctatcaa
    10321 cgaatcagac gtgacccttg ccgaagcttc aaatgccttt atcgttggtt tcaacgtacg
    10381 ccctacacca caagctcgtc aacaagcaga agctgacgat gtggaaatcc gtcttcacag
    10441 cattatctac aaggttatcg aagagatgga agaagctatg aaagggatgc ttgatccaga
    10501 atttgaagaa aaagttattg gtgaagcggt tatccgtgaa accttcaagg tgtctaaagt
    10561 gggaactatc ggtggattta tggttatcaa cggtaaggtt gcccgtgact ctaaagtccg
    10621 tgttatccgt gatggtgtcg ttatctatga tggcgaactc gcaagcttga aacactacaa
    10681 agacgacgtg aaagaagtga caaacggtcg tgaaggtgga ttgatgatcg acggctacaa
    10741 tgatattaag atggatgatg tgattgaggc gtatgtcatg gaagaaatca agagataaga
    10801 ttttttgctc ctttcttagg tggtgaggga cgcaagcaaa ccgatggttt cattgcttat
    10861 ttttgagcct agggtctcaa aaatcccctg tgatgggact gataaatcag ttccatcact
    10921 ttcaccacgg cgaaagaagc agatgacttc aaattgaact tcgtttcaat ttaaactgaa
    10981 aatcaagaag tttaaaatag ctaggtctgc tggcctagct tttggttcaa agtagagaaa
    11041 ggaatatcat ggcaaatcat ttccgtacag atcgtgtggg catggaaatc aagcgtgaag
    11101 tcaatgagat tttgcaaaag aaagtccgtg atccacgtgt ccaaggtgtg accatcacag
    11161 atgttcagat gctgggtgac ttgtctgttg ccaaggttta ttacaccatt ttgagtaacc
    11221 ttgcttcgga taaccaaaaa gcccaaatcg ggcttgaaaa agcaactggt accatcaaac
    11281 gtgaacttgg tcgcaatttg aaattgtaca aaatcccaga tttgaccttc gtcaaagacg
    11341 agtccatcga gtatggaaac aagattgacg agatgctacg caatctggat aagaactaaa
    11401 gaagaggggt tgcccctctt ttttggtgga ggaaaatagg ttgaatttga aatggaaaaa
    11461 tattctttta aaatatattg aaactagaat agtacgcctc tacttctaaa acatt
//
LOCUS       DAVLWA010000056        11111 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLWA010000056 DAVLWA010000000
VERSION     DAVLWA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.056"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(443..1636)
                     /locus_tag="VYN80_001435"
     CDS             complement(443..1636)
                     /locus_tag="VYN80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1541334.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1774..3216)
                     /gene="gtfA"
                     /locus_tag="VYN80_001436"
     CDS             complement(1774..3216)
                     /gene="gtfA"
                     /locus_tag="VYN80_001436"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1541335.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(3493..3573)
                     /locus_tag="VYN80_001437"
                     /pseudo
     CDS             complement(3493..3573)
                     /locus_tag="VYN80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3574..4407)
                     /locus_tag="VYN80_001438"
     CDS             complement(3574..4407)
                     /locus_tag="VYN80_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1541336.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4421..5287)
                     /locus_tag="VYN80_001439"
     CDS             complement(4421..5287)
                     /locus_tag="VYN80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1541337.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNTIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(5301..6560)
                     /locus_tag="VYN80_001440"
     CDS             complement(5301..6560)
                     /locus_tag="VYN80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1541338.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6652..8814)
                     /locus_tag="VYN80_001441"
     CDS             complement(6652..8814)
                     /locus_tag="VYN80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1541339.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8922..9782
                     /locus_tag="VYN80_001442"
     CDS             8922..9782
                     /locus_tag="VYN80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1541340.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9779..10714
                     /locus_tag="VYN80_001443"
     CDS             9779..10714
                     /locus_tag="VYN80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1541341.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            10860..>11111
                     /locus_tag="VYN80_001444"
     CDS             10860..>11111
                     /locus_tag="VYN80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541342.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNKDTHKEIIAKLDYDAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYH"
BASE COUNT         3601 a         2359 c         1910 g         3241 t
ORIGIN      
        1 attttggaat tcaaattaaa ttataacaat attgtagaag tatcattcta gtattcaaga
       61 ttcagtttac tatgtctttt cacaccaacc ttattccgaa ttcaattact ttcgtgattt
      121 acacatatag attaagacta tcttttatac tttaaaattt ctcgctacct tgtccactat
      181 atgctcctcg ctatcacgtt tctattcata gcctacgatt tcactattgc tttctctgac
      241 aattcttatt tcctgcgtca gacttaaaac gatctatccc cagaccattt taatccgcta
      301 cctcacgata gtcaggcttg gggagcgcta ttgtattcac cggtagtgga tccctacaga
      361 ggacttacac ctcagatgca cgacatgccc atcgtataaa aaaatctcct acccaaggta
      421 gaagatttca aacttataaa acttaatccg tcatgtccga taccaacatt cgatgctcca
      481 atggaatact gcacataact agcaagaaaa taaagcctga ctgaatccag aagagagcca
      541 agtcaaaaat tccgtgcaca gcaaccactg taaggaaaga tagataaagg ccgataatcg
      601 gacgtttccc cgactcctga ctcatatcca tcatcaagcg aacaggagca acagaagaca
      661 aaactaataa aatggtaccc acaattccgt aactcagaat cgtatcaata taaagactgt
      721 gggcatgttc atgataagga gcatgtatcc gaggataaga gtgcatatag gtcaatggcc
      781 cttcacccca aaaaggattt tgcttaaaca aggccatccc agcatcccag atagaaatgc
      841 gttcttccat agaagagtct aaagtaccca ttcgaactcc caaatcacta gaaaagagga
      901 aactcaaacc aatcgcgaag accccaatac taagccaaaa ggccttccag tttttaatag
      961 tcgtaaagag atagataatt gctccagcga taatagcagg aaaggcagtt cgattttgag
     1021 taaagttcaa accaaagaga ttaacaaagc ctgcaatcac acagaatact ttcaaccaat
     1081 tcaacttggt cgttgtaaac agatagaaag caatcataat acagaaacaa caaataattc
     1141 cataataatt aggattaaag aaggtcactt ctgcccggtt ctgatgccac acctgcatat
     1201 tgggtgaaag aaaagcatag ttaaatttct tcacaatttg gaaatgttct aaactggcaa
     1261 aagcagctga caagacacta ccaaacaaga cgaactgcaa aatcaatcga aagaatttat
     1321 gggataaaat cgactgatag tgcaaaaaga aaatagtaaa tagaaacatt cctactgaag
     1381 ccacaagacc catccaattt tgtgcaagaa tggatataac agtactatag ctaagaaaaa
     1441 gaagcagcat cggatgctcc cccattttct gaagaatact tttcatgtct cctgtaaaaa
     1501 tcaaactgat aatatataaa cagagtacaa ctacaaaaag ataaaagggt aaaaagatac
     1561 ttaggataat tcccaataaa atcagctctt tactagacaa ccccttcagc ttttcaataa
     1621 agcctattga tttcaaaatg aatcctttct ctccaaatca gctgattcag ataatagtaa
     1681 gctatcctat attgtaccac ttttttagca atttgaaaac aaaggaaacg ttttccaaaa
     1741 taaaaaccct attttatcca ccatatcaaa gcttcaaaat gatacttcaa ctccattctc
     1801 aattacccga taagtctgat tttgcaaatc aatttctact actgctgtta cggacttatc
     1861 tttattttga cgtttgatta caatgctgtg agctgttggt gtctctatct cagtagtccc
     1921 ttctagatca aaggcttctg aacggttacg gaaagaaaat agattgagaa gggccttcac
     1981 aacaggtcgt tgcacttctt ttgctatttc ctcgttgcta tagtaatgac gattaatatt
     2041 tcgaccttct ttagtttctt ctaataattt caagtcattc ttgcctgcta atagacccac
     2101 atagtaaacc tgaggaatac ctggggcaaa agcttgaatt agacgagcga gaaaatactt
     2161 gacatcatca tctccaagcg ctgaatagta ggttgaattg atttggtaga tatctaagtt
     2221 gttatactcg gcactagagt acttacgttt gacattggct ccaaccttat agagttcatt
     2281 tgaagcatag tcaatctcct catcggtcag gatatccttg acatctacta ctccaatccc
     2341 atcatgggta tctagcgtcg taaattgctt catcgggctc atctttaacc acttagccaa
     2401 acgctctgtt ctggaactgt aaagagtata aagtgtcacc attggaagag caaaatcata
     2461 aacatagtaa tcatggtctg ctattttaaa ctgaatcgaa tagtgttcat gaatctcagg
     2521 taaaagctct gtcccatact cagcagcgat atctcgaact ttgtccaata aatcccaaat
     2581 atctggttcc acaaagaaat cattagtatc caatttcttc actgcataag caaaggcatc
     2641 tagacgaatc aaatcacacc cattacttgc caagtgctga atggtcttac ggataaattc
     2701 catagttact tctttggtca catcaagatc aatctgctcc tcaccaaagg tattccacaa
     2761 atgttccact gaaccatctt caaacacaat ctcttgcttt ggtgcacgat ccttacgctt
     2821 gtaaattaaa tctacatcag actgtgtcgg acggttttct ggccaaaact tatcccagtt
     2881 taaaaagaga gctttaaatt cactggcttc atgtttttct tgatagtcct tataatactt
     2941 ggattgacga gaaatatgat taatcataaa atcaaacata agataatatt tctcacctaa
     3001 acgcttaaca tcctcccaat caccaaaagc tgagtccact tcgtcgtagt caactggcgc
     3061 aaatccacga tcacctgttg atgggaaaaa tggtaaaagg tgaactcctc caatagcatc
     3121 tccaaaatgc tcttccaaat tatcatataa gtctttaaga ttatttccaa ggctatcaga
     3181 ataggtaatc aacatggttt tattttgaat cggcatcatt actctccttt ttctaattga
     3241 agccaagtct catatgatct ggcttcgtaa atcaaattca ttttaaatct ctatttatca
     3301 tcaaactcgt actaatatag actgtgataa acaaagtact actttcttgt tttctacata
     3361 gaattatcaa caagctgaac tcttcctctg tgtcaaagac tatagattcc atgagctctt
     3421 cttatactct tcgaaaatct cttcaaacca cgtcagcttc accttaccgt aggtatggtt
     3481 actgacttcg tcagtttcat ccacaacctc aaaacagtgt tttgagcaac ctgcggctag
     3541 cttcctagtt tgctctttga ttttcattga gtattacttc actgccccgt tgctcattcc
     3601 tgaaatgata tggcgttgga agaagagata gacaatggtg atactgataa tgccgaccac
     3661 gtaagaggca aagcttggtc cgtagtcgtt gaaatattgg cctgcgtagt tgtattggaa
     3721 caaaggcaga gtccacattt tggaatcccg gttcaagaca aggagtggca acatgaagtc
     3781 attccagaac caaagggcat tgatgatcat ggttgtcgca tgcatcggtt tcatcattgg
     3841 gaagatgatg cggaaatagg ttgtaaattg attagcccca tcgatctctg ctgcttcatc
     3901 cagactttct ggaatcgaga ttttgatata gccaacatag agaaagaggg tctgtggaat
     3961 cgcataggtc aagtagagca agatcaaacc aaaggtatta gccaaaccga gtttactcat
     4021 cataaccgta atcggaatca tgatgacttg gaaaggtacg aagattccga ggattaagag
     4081 ggtatacatg atggtaaaga cttttctttt actcatattg cgagcgatgg agtaggctgc
     4141 catagggata aagatcatta ctgcaagtaa agacaagaca gtgatgacga cagagttcca
     4201 ataatagcct ccaatcccat cagctaagag acggctaaag ttgtcccatg tgaagttggt
     4261 tggaaagcca aagaaattat ctacaatatc cttagtgggt ttgaaggaac taaagagggt
     4321 agcaaggagc ggcactaaaa tcagaaccga tcctagaatc aatagaatgt atttgccaat
     4381 cagggctttt ctttcatctt gtttcatcat gcttctcctc ttaaatttca aatttcttag
     4441 atactctcaa ttggatgatc gaaatcacta caattaagag gaacaagatt acggcaatgg
     4501 cattggcata accgaattgg ttgtttttaa aggcatagtt ataaaccaag agcccaagtg
     4561 aggttgtggc attgtttgga ccaccaccgg tcatggcaaa gacttggtca aaggcagtca
     4621 gcccaccttt tagggctagg ataaagacca tagagacact tggtagcaag taaggcaatt
     4681 caatgttcca gaaaacttgc ttgctagtcg caccatcaat ccttgctgcc tctgtaatct
     4741 cagttggaat agattgcaaa ccagctagga agatgatgat gggcatagcc accccttgcc
     4801 aaagaaggac aaagacagcc gcaaagattg ctccccactt agtccctaaa agactggttt
     4861 ggagaaattc aatatgaagg gcattcccaa tcgctggaag accgtagttg aagacttgct
     4921 tgaagatcaa agccactgtc aaaccagata aaacagctgg gaagaagaac caagcacgga
     4981 agaaggtttg gcctttgatt ttagagttca agacacgcgc aatgaagatc ccgagtgcaa
     5041 tctcaccaac caccatggca atcgtaatga ttgcggtaaa gccaatcgta ttcatgaatt
     5101 ttggatccat gaagaggagc ttaaagttgt ttaagccgac aaatttgtag ttgtaagtca
     5161 atcctgtcca gttggtaaaa ctgtaaaagg ctccttgaaa catcggcaca tagaagaaaa
     5221 ttgcttgtaa caagaggggg atgaccacaa aagcccatgc ccaatatttt tgtaatactt
     5281 ttttcatagt ctctctactc ctaatccaca tccgctttca tcgggttaaa gaaggcattc
     5341 aaatcattga ccatgccttg tttatcaccg gtcaagacat agttcatggt caaggtatgg
     5401 aagtctgctt cactggtcca gtattgttgc aaccagacca agtgacgatc cgtaaaggca
     5461 tattcggtca taccagcaag cggtgaatct tctcctgctt gtttgacccc ttcgatcgct
     5521 gttggagatc cgtccacatc gtagtatttt tgcatgactt ctggacgggt catatattcc
     5581 acaaaggcat tggcttcttt tggatgtttg gtggtggctg agatagacca tgccaagtct
     5641 cccgcaccaa cggttaagct ttgtcctttt tcttttcctg gaatcatgaa ggttccaatc
     5701 ttaaagttcg gtttttgttc attaatcgct gtgatcgccc aagacccatt tggtgtcatg
     5761 aggacatccc cacgtgcgaa ggctccgata acatcggtat agccagcacc ttcccagttc
     5821 ttttgcttag agccattgat gcgaaggatg tccatgacct tgatatcatc tttcataatc
     5881 ggatccgaca atttaatggc atttggttga gaataacgaa ggtattgatt tgcttctttt
     5941 cctccacctg ttgctgtcgc aaaggctaat tgattgtaac cattgagtgt ccaagcatct
     6001 gcacctgcaa ttccaaatga tgtttgtcct ttagcaacga tatctttgac taactgttca
     6061 aattcatccc aggtttcagg aaccttcaag cccagttctt cgaatttatc tttgttgtag
     6121 taaattccat aagcattagc tgtaaaagga acgttataaa ctttttcgtt tacagcatat
     6181 ttttcagcgt aggcattttt cacgcgtttc aggtagtctt tgttgctcaa atcttcaaaa
     6241 acacctgctt ttgcccattc ttgcagttcg atggactgtg ggtaaatatt gaccacatca
     6301 ggcacatctc ctgcgagaac gcgtgtcttc aatacttcac cagcatttgg tacattgacg
     6361 actttgacct tgatcttagg gttttccttc tcaaaatcac gagtgatttc ttccaaggtt
     6421 ttggtcattt cttttttctg gttgaaatac tcgatggtca ctgtgccatc cgcagattta
     6481 ccatagttgg agcaagcgcc gagcccaaac aaagctaaac ctgtagttgc aagaagtccg
     6541 atttttttat accattccat tagaaagcct cctttataaa tttatacacc cttattgaac
     6601 tgcaccccaa aagttagaca gaataaatct aacttttggg gtcagtacat atcatagttt
     6661 tctaaaaata tactgtctac tcaaaaaatc tccttgggat aagataacag ttaagcccgc
     6721 atacattagt tctgcacctg agtaaacttc gccattttcc tgtaatttat atagtccctc
     6781 ttcatccaaa tcttttaatt ttaaagttgt ttccatggtc tctacaacag ataaaacgcg
     6841 aacgtaggtt acaatcgttt gatttccgta attaaattgt acagctgctt cattggatgc
     6901 agtatcagga ttaattagtc tatactgctg tcctaactga actactggtc gtaattcttt
     6961 atacaagttc acctgattag caatcgtagc tttctcttca tctgataaat ttgtcaaatc
     7021 aagctcatag cccaaatttc ccatcattgc tacatggcca cgtgtttcta atggtgtcat
     7081 tcgtcccatc tgatgattcg gtactgctga cacatgagcc cccatagaaa tggttggata
     7141 gagataggat gaaccgtatt gaattggtaa acgtgcaatg gcatcagtat tatcactagc
     7201 ccagacttgt gggaaatagc gtatcatacc aagatcattt cgtccaccac caccagagca
     7261 ggactcaaag agaatatggc tgtgcttctc tgtcagataa gaaacgagtt cgtaaagccc
     7321 cagcatgtac tgatgagatt gcatctgtgt ctctagataa gttaatccat tccctagctt
     7381 agtgatattg cggttcatat cccatttaat gtaatcaatc tcatgataag ataggagttg
     7441 atctaagaca cttttcaagt attctactac ctgaggattg gcaagattaa gtactaattg
     7501 attccgagaa taagtatgct cataatcagg aacctgaata gcccagtcag gatgttgacg
     7561 atacaaatca ctatctacag aaatcatttc gggttctaac caaagtccaa actgcaaacc
     7621 tctttcatgg atagctgaaa tcagactttc tagacttcca cccagttttt cctcattaac
     7681 aacccaatca cctaaagcac gattatcatc aaaacgattg ccaaaccaac catcatctaa
     7741 tacaaaaagt tcaatgccaa ctttcttagc ttcatctgct aactctaaca gtttttctct
     7801 ctgaaagtca aagtaagtag cttcccagtt attgattaga attggacgtt cttttttaga
     7861 aaattcactt agcataatgt gcttcagtac aaaattctga ctttcatgac taataccagt
     7921 taatccctga tctgaatgag tcactaaagc taccggtgtt tcaaagtatt cctcaggggc
     7981 taacttccaa gaaaagtttt ctggattaat gccaatagcc acccgaactt cattcaattg
     8041 atttttttga acaaaagctt caaagttgcc actatacatt agttgaatag caaacacatt
     8101 cccagcatcc tctgtgactc cttgttcgca tagtagaaga gctggtgttt gagcatgacc
     8161 agaagcacct cggtttgaac taatcgaaaa gattccttgt tctacctgtt gacgtctaac
     8221 agtcttttca cgagcataag caccctgcag agttgctatt tcataatctg cagctggaaa
     8281 atcagccata aaagaaaaat ctttatggat gacaacttcc tgattactat tattatctaa
     8341 tttactgtag ctagtaatag tcgcatcatt attaaaagta gtataataca aagtcagact
     8401 aagttgagcc ttagaatctt ctaacattaa gacaagagtc tctgtatcgt ccatgctatg
     8461 tggagaaggt aagccctgtg gaccattctg accttttaaa atctttgctt ctacaaatcg
     8521 aaagtctgtt acttcagtta cactatgctg aacctgtata gttggtttcc taaaatctcc
     8581 taagccatgt tgtccaaaaa tctgtcgctg agtatctaaa ctaaaggttc gattagtagc
     8641 cgttggattt cctgaaaagg catggtctcg ttcataaaca ctattggaac ctttatagtt
     8701 cttaatagtc tttcctaaat gtttcaaaag taagtagcca tttcgatttt caataatcaa
     8761 acttagattt ttactctcaa cataaaatag attattctct atcctaactc ccatttactt
     8821 cacctcatca ctttattgat tatattttat cacctgaaat cgctttccaa aatagaaaaa
     8881 tgtctcaaga atatggtaaa atgttaggta ggaggtagca catgttagtt ttttcagaat
     8941 accagactgg aacaatcgac cttgccctaa gcttttatgg atatgaggaa tgcacaccta
     9001 attactcttt tggtccagcc attcgtgata catacgtcct acattacatt actaaaggac
     9061 aaggaaaatt tcattacaag gataaaattg ttgatttaaa agaaggagat ttctttctat
     9121 taaaaccaga ggaactaacc ttttatcaag cagatagtaa agaaccttgg gcctactact
     9181 ggttaggaat cactggaggg aaatcccctg attattttgc tctttctcaa atttctgatc
     9241 aatcctatct catccaatct gaaacttgtc atacccagac tactgcaaaa ctcatctcag
     9301 acattgtccg cttcgctcag attacaaaat caagtgaatt agctcaactc catatcatgg
     9361 gacaacttca tgaactgatg tttcatctgg gaactattgc tcccaatcag aaaaaaaaga
     9421 atatttcatc aacccaccaa ctctatcttg aatgcaaacg attaattgat agccactatc
     9481 ctcaatcact tacaattcaa gatttagcaa aagaactatc cgttcacaga agctacttat
     9541 caagcgtatt caaagaattt aataccttat cacccaaaga atacctactc tacgttcgaa
     9601 tgcaccgagc tagacaactt ctcgaaaata cccaagagtc catcaaggta attgcatact
     9661 cggtaggttt ttcagatcca ctccattttt cgaaagctta taaacaatac tttaatcaga
     9721 ctccaagtca tacaagaaaa gaatactctc aataccaact agtgagaaag gcaacattat
     9781 gaaatcctac caagctgtct accaaatcct atctaaagaa accgactata tcagcggaga
     9841 aaaaatcgca gaaaaactat ccctaagccg aacatcaatt tggaaagcta tcaagcgact
     9901 agaacaagaa ggcattgaaa ttgatagtat caaaaataga ggatataaac taatgaatgg
     9961 tgaccttatt cttccagaga ttctagaaga aaatcttcca attaaagtca gctttaaacc
    10021 cgaaacaaaa tcaacacaac tagatgcaaa agaagcaatt gatttaggcc atgaagcaaa
    10081 taccctctat ctagcttcct atcaaacagc aggccgagga cgttttcaac gttccttcta
    10141 ctcaccacaa ggtggtattt atatgacact ccatcttaaa ccaaatctcc cctatgacaa
    10201 attaccatcc tacacactac ttgtagctgg agctgtctac aaagctatta agaacctaac
    10261 tttaatagat gtcgacataa aatgggtcaa tgatatctat ctaaacaatc ataaaattgg
    10321 aggaatcctt actgaagcaa tgacctctgt agaaactggc ttagtcacag atatcattat
    10381 tggagtaggt atcaatttca ctattaaaga cttccctcag gaattaaaag aaaaagctgc
    10441 cagcttattt aaagctacag ctcctataac aaggaatgaa ttgatcatag aaatctggcg
    10501 tgctttcttc gaaacaccag cagaagagct attataccta tacaaaaaac agtcattcat
    10561 tctaggaaaa gaagtcactt tcacactaca gcaaaaagac tacaagggac ttgctaaaga
    10621 catctcagaa aatggaaaac ttttagttca atgtgataac ggaaaagaaa tctggctaaa
    10681 tagtggcgaa atttctctca atagttggaa gtaaaataac acaattataa tataaggctc
    10741 tataatattt gtagtgggta aatcccctat ggatattatg gagcctattt ttgtgtagaa
    10801 aaaaagtccc ataagatcta taatgaaaag cgatcaaaca actcattaga aagattcata
    10861 tggaacaatt acattttatc acaaaactac tcgatatcaa agacacaaat atccaaatta
    10921 tagatgtcgt caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc
    10981 catcttgccc tgagtgcgga agtcaaatga agaaatatga ctttcaaaaa ccttctaaaa
    11041 ttccttatct tgaaacgact ggtatgccta ctagaattct ccttagaaag cgtcgattca
    11101 agtgctatca c
//
LOCUS       DAVLWA010000057        11014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLWA010000057 DAVLWA010000000
VERSION     DAVLWA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.057"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1070
                     /locus_tag="VYN80_001445"
     CDS             <1..1070
                     /locus_tag="VYN80_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1541343.1"
                     /translation="FQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKK
                     NHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSW
                     DEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIRDHFLKYDRAVRCRVKIITMDMFS
                     PYYGLAKQLRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSD
                     KQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNL
                     KQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFK
                     KRIFIALNIKKERTKFVLSRA"
     gene            complement(1170..2792)
                     /gene="groL"
                     /locus_tag="VYN80_001446"
     CDS             complement(1170..2792)
                     /gene="groL"
                     /locus_tag="VYN80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1541344.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2808..3092)
                     /gene="groES"
                     /locus_tag="VYN80_001447"
     CDS             complement(2808..3092)
                     /gene="groES"
                     /locus_tag="VYN80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1541345.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3265..3660)
                     /locus_tag="VYN80_001448"
     CDS             complement(3265..3660)
                     /locus_tag="VYN80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1541346.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3738..4499)
                     /locus_tag="VYN80_001449"
     CDS             complement(3738..4499)
                     /locus_tag="VYN80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1541347.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4532..5161)
                     /locus_tag="VYN80_001450"
     CDS             complement(4532..5161)
                     /locus_tag="VYN80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1541348.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKKENG
                     ETVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEI
                     VEMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPG
                     ELRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(5177..5494)
                     /locus_tag="VYN80_001451"
     CDS             complement(5177..5494)
                     /locus_tag="VYN80_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1541349.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5491..5790)
                     /locus_tag="VYN80_001452"
     CDS             complement(5491..5790)
                     /locus_tag="VYN80_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1541350.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6075..6266)
                     /locus_tag="VYN80_001453"
     CDS             complement(6075..6266)
                     /locus_tag="VYN80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1541351.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6427..7101)
                     /locus_tag="VYN80_001454"
     CDS             complement(6427..7101)
                     /locus_tag="VYN80_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541352.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTAKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(7107..7553)
                     /locus_tag="VYN80_001455"
     CDS             complement(7107..7553)
                     /locus_tag="VYN80_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1541353.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7667..8170)
                     /locus_tag="VYN80_001456"
     CDS             complement(7667..8170)
                     /locus_tag="VYN80_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1541354.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISVACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8167..8529)
                     /locus_tag="VYN80_001457"
     CDS             complement(8167..8529)
                     /locus_tag="VYN80_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1541355.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8595..10460)
                     /locus_tag="VYN80_001458"
                     /pseudo
     CDS             complement(8595..10460)
                     /locus_tag="VYN80_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10470..10940)
                     /locus_tag="VYN80_001459"
     CDS             complement(10470..10940)
                     /locus_tag="VYN80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1541356.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
BASE COUNT         3223 a         2598 c         1992 g         3201 t
ORIGIN      
        1 actttcaaaa accttctaaa attccttatc ttgaaacgac tggtatgcct actagaattc
       61 tccttagaaa gcgtcgattc aagtgttatc actgttcaaa aatgatggtc gctgaaactt
      121 ctatcgtcaa gaagaatcac caaatccctc gtattatcaa ccaaaaaatt gcgcaaaagt
      181 taattgaaaa gatttctatg accgatattg cccatcagct ttccatctca acttcaactg
      241 ttattcgtaa gctcaatgat tttcacttta aacatgattt ttcttgtctt cctgagatta
      301 tgtcttggga tgagtatgct tttacaaagg gaaagatgag cttcattgcg caagatttta
      361 acaatctcaa tatcatcact gttcttgagg gaagaacaca agctgtcatc cgagatcact
      421 ttcttaaata tgatagagcc gtccgatgtc gcgtcaaaat tattactatg gatatgttta
      481 gcccttacta tggcttggct aaacagcttc gctttcacat tgtacaacat cttagccgtg
      541 ctatgagtcg tgtgcgtgtc caaatcatga atcagtttca tcgaaaatcc catgaataca
      601 aggctatcaa gcgctactgg aaactcattc aacaggatag ccgtaaactg agtgataagc
      661 aattttatcg tcctactttt cgtatgcatt taaccaataa agagatttta aacaagcttt
      721 tgagctattc ggaagacttg aaacatcact atcagctcta tcagctcttg ctttttcact
      781 tccagaataa ggaaccagag aaatttttcg gacttattga ggacaatctt aagcaggttc
      841 atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag attgtcaacg
      901 cccttcaact acactattct aatgccaaac tggaagcgac caataatctc atcaaactta
      961 tcaagcgcaa tgcctttggt tttcgaaact ttgaaaactt caaaaaacgg attttcatcg
     1021 ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag cttttcttca
     1081 acccactaca gttgacaaag agcctataaa aagaagggtt agtcattccc tccttttgtg
     1141 ttttttataa gttgttttct atagaaagct tacatcatcc cgcccatcat acttggatcc
     1201 attgctggag ctggggctac tggttctggt ttattggcta cgactgcttc tgttgtcaaa
     1261 atcaagctgg ctacagatgc tgcattttgt agggctgaac gactcacttt aactggatca
     1321 atgatacctt gatcaatcat gttaacccac tcgccagttg ctgcgttgaa tcctatacca
     1381 agctcagcat ttttcaaacg atcgataacg atagatcctt caaatcctgc attgtgagca
     1441 atttgacgaa cgggttcttc caaagcacgg agaacaatat tacgtcctgt tgcttcatct
     1501 cctgtcaatt ccaaggtagc aacagctgga atcacattgg caagagctgt tccaccacct
     1561 gcaacaatac cttcttcaac agctgcacga gtagcgttga gggcatcttc aatgcggagt
     1621 ttcatttctt tcaactcagt ttcagttgca gctccgacct taataaccgc tacaccacct
     1681 gacaatttgg ccaagcgttc ttgcaatttt tcacggtcaa attcagaagt tgtagtttcg
     1741 atttgagact tgataaccgc aacacggtta gaaatcgctt caggatttcc tgcaccttct
     1801 acaataaccg tgctatcttt gtccacggtc actctcgctg cttgaccaag agcttcaatt
     1861 gtcgcatctt tcaactcaag accaaggtct tctgtgataa ctgttccgcc tgttaagatg
     1921 gcgatatctt caagcatggc tttgcgacgg tcaccaaaac caggtgcctt gactgctact
     1981 acgttgaagg ttccacgaat cttgttcaaa acaagagttg gaagagcctc gccatccaca
     2041 tcatccgcaa taatcaagag tggacgattg ctttggagaa tgctttccaa aagtggcaag
     2101 atttcttgga tattggaaat tttcttgtct gtaatcaaaa tgtacggatt ttcaaggtca
     2161 gccaccattt tttcgctatc agtcaccatg tactgtgaaa ggtaaccacg gtcaaactgc
     2221 attccttcta cgacttcaag ctctgtttcc ataccacgtg actcttcgat ggtgatgaca
     2281 ccgtctttgc caactttttc cattgcttca gagatgtact caccaacttt ttcagaacga
     2341 gaagatacgg ctgcaacttg agcgatagct tctttattgg caacagggat ggcgttgttt
     2401 ttcaaagctt caactgctgc ggcaactgct gtttcaatcc cacgacgaat accgattgga
     2461 tttgcacctg ctgtgacgtt tttgattcct tcacggacga tagcttgggt caagactgtt
     2521 gcagtcgtag tcccgtcacc tgcgatatca ttggttttag aagctacttc tgatactaac
     2581 ttagcaccca tattttcaaa atggtcttcc aattcgattt ctttggcaat ggtcacaccg
     2641 tcattggtaa tcaagggtga accgaatgac ttttcaagaa cgacattgcg accttttggt
     2701 cccaaggtta ctttaacagt gtctgcaagg atatcgacac cacgaaccat ggctgaacgg
     2761 gcatctgatg aaaatttaat ttcttttgac atacttactt tctccttcta ttcctcaatg
     2821 attgccaaaa tgttagcttc gcctacgatg atgtactttt catcgccatc tttgacatca
     2881 agacctgcgt gggcttcaac taagacacga tctccagttt taacacttgg agcaaccaag
     2941 tcaccgttca aggtacgaac accttgtcca gtagccacaa cttgggctgt tttggttttt
     3001 tcttgggctg aacctgcgag aacaaagcct ccaacagttt gttctttttc ttctactttt
     3061 aagaccacac ggtcccctaa tggtttcaac atctgatttc ctccataatc tgatttcctc
     3121 cataatgaga tagatattat tagcactctt tatacctgag tgctaattca tagttctatt
     3181 gtatcactta gtcagaaata atcaagaaaa aagtctgact ttctcaagat aaaaagcctg
     3241 agaccaactc agacttttta attcttaaaa tggcaattct tcctcttcca agaccaaatc
     3301 tgccaaatct tggcctgcat tattttcacg catagcacgt tgggcacgac tttccaagag
     3361 ttggaatcct gtcacaagga cttcggttac ataattcatc tgaccatttt tctcaaagcg
     3421 acgggtacgc aattctccat caacggaaat gagactacct ttggttgcgt agcttgccaa
     3481 agtttctgct agtctgcccc atagaaccat attgacaaaa tcagcttcac gttcaccgtt
     3541 ttggtctttg taacgacggt tgacagcgat agttgctcgc gctactgact tgtcattgtt
     3601 ggttttgtgc aattctggtg tagacgttaa acgtccaatc aagataactt tattatacat
     3661 attttcttcc tcctacttat ctattcgtag gaaatcaaaa aaagttacag aaatttgtaa
     3721 cttttcgaga aaatttttta ttttttatga accatgaaac ctgtcgcctg ttgattggcc
     3781 ataatggtca tatctgtaat ctgaacacga cgaggttgac tagtcacata gactactgta
     3841 tctgcaatat cctgagcttg caaagcttct attccttggt aaacggacgc agctcgttct
     3901 ttatcaccat gaaaacgcac tgtagaaaaa tctgtttcga caattccagg ctgaatggtc
     3961 gtcaccttga tatccgttgc gatggtatca attcgcagtc catccgaaaa ggtcttaacc
     4021 gctgccttgg tagctgagta aacagcggca ccagcatagg cgtagattcc tgcggttgac
     4081 cccatattta taatatgacc ttgattgact tttatcattg ctggcaagaa acagcgagta
     4141 accgccatca gacctttaac attggtatcc aacatggtca acatatccaa ctcctcatag
     4201 tcttgatagg gagccaagtc aagagccagt ccggcgttgt tgaccaagat atcaatcctc
     4261 cctatcgttt ctagaatatc agagcaaaca gtcttcacca tgaccatatc cgtcacatct
     4321 agtggaaaag tccagacggt ttgatttgga aagcttactg caaactccga cttgaggatt
     4381 tctaatctgt ctatccgtcg tcctgttaga acgacatcct caccctgctc caaataagca
     4441 cgcgcaatcg cttcaccgat tcctgaggtt gctcccgtaa tcactacatt ttttgccatc
     4501 ttatttcctt ctatcagatg ctgacaagtt cctaggcagt ccagtgtttc gctgggtcga
     4561 acggtgttcc gacaacttgg tcttctgata attcaagcac cccacgtttt tgcggagcat
     4621 ttggcaaatg caattcacga ggactacaca tcatcccaaa actcttttca ccacgaagtt
     4681 cgcctgggaa aatgaaattc ccttttggca tcatggcacc aggaagagcc acaatggttt
     4741 tcaacccaac acgcgcatta ggtgctcctg caacgatttg cactatcttg tcacttgcga
     4801 ctgcaacttg gcagatgttg aggtggtcac tgtctggatg agctaccatc tcgacaattt
     4861 caccaacaac aaacttaggt tccttatcat taacaatttc ttctgtaaaa ccttccgcct
     4921 gtaattcttg gttcaaacga gcgacttgct catctgataa aaagacttga ccgcgctctg
     4981 caatttcaaa caagctcgaa acctcaaaaa tgttccaagc tactgtttcc ccattttctt
     5041 ttttgaggaa aacacgggct accttgcctt tgcgttccac attcagtttg gcatctccgc
     5101 tatttttcac gatgaccata aggacatcac cgacatgttc tttgttatac gtaaaaatca
     5161 ttgtttcctt tttctcctat ttcaatcctg ctaaaaaatc gttaatttgc tccttacttt
     5221 tacggtcgcg attgacaaaa cgaccgattt ccttgtcctt ttctagaaca acaaggctag
     5281 gaattccgta aacatcccag agtttggcca aatccatata ctgatctcgg tccattcgaa
     5341 taaaggtgaa ctctggattg gtctcctcaa tctctggtaa ggcaggataa atataacgac
     5401 aatcgccaca ccagtctgcc acaaaaagga agaccttctt gcccgctttt tccactaaag
     5461 atgctaattc ttctaaactt gctggctgta tcataagact tcctcctcat agactaggtc
     5521 ttcattttca tagacaaagg tataatgacg gccatcctca aaaatgacgc caccaaccaa
     5581 gctctccaga ctgctttcgt aaacttgaac atagagggtc gcaatttccc ccatgtcgga
     5641 aaaatggtct cgcacaatct ctgtcaactc ttcctgagtc ttcatgagct tacggtcatc
     5701 tgcaactttt ttcgtggcaa gagcaaggct tccgatacct agcagagcca agcctgccat
     5761 ccacattttt ttagctttca taccattcat tttaacacaa aaaaggcttc aggacaaatg
     5821 aggaagcagc agaaaagcaa gtaaaaagcc tcttccttta aggaaaagga cttcttatac
     5881 taagaccaaa ctagaaagct agccgcaggc tgctcaaaac acggttttga ggttgtagat
     5941 aaggcgacgc tgacgtggtt tgaagagatt ttcaaagagt attattctta ttgccaggca
     6001 cctaagttgc caacgtagta accatcaggt gtgtaggtat tgcgaaacat cttacctgat
     6061 gaagccagat aatactactt gccattgtct ttgacccaat cattcgcaat catggaacca
     6121 gaagaactta cataatacca ttctcccttg tcataaaccc aagtactgac tttcatggtt
     6181 cctgagcaat taaaggcaaa aaaactgtcc aatatcattc gttttttaaa agcatttgac
     6241 actacatcat ctacgctgta gtacatagat acatcaaacg taaaagccaa aatatcgaaa
     6301 aatatacaac ttttaaaggc agcatttcat tttttaaagt ctaacaacca tagaactgaa
     6361 atcagagcaa ggcatcaatt tcctactctt agtacgtcta ttttgatggt tgaagtgaag
     6421 atttttttat ttaacgtaga caatttccaa cttcccaaaa gccacatctc cacggatagt
     6481 caaggttttg ctggttgggg ctacaggagc gtcagcctta gctgcaccaa aggaggtttc
     6541 tactttcaaa tctacacgcc agtgttgtgg aacatagacg gttgcatttc cgaaggccac
     6601 ttcaatattt aaagttgcaa aatcacctag catctctgca ttatcatagt agattttagc
     6661 atccccaaaa gcgacttcca cttggtcatc tacgagatct tgatcttgct tatagaaggt
     6721 cccgctacca aaagcgactt ccttagccgt gacgactgtt tttttcccat tgtaccacca
     6781 ttttttttca ttccagaact tacttgaatg cgtcagataa ccaacaccaa gtaccaccaa
     6841 gatgctagcc caaataagag aatgattggt aactggtaac aagtcataag cgtaatttgc
     6901 aatgatgagc gccagtaaac ctgtaaaaac tgccgaagtg agatgacgtc taaggatgga
     6961 ctcaatggac ttataagcaa aaaaaacaat acctagtaaa ggccatattt taccatccaa
     7021 agaaggaatt ccaaaattcc cttgcagcaa gatccaagct gctaaaacca ataaaacaat
     7081 accaaatgct ttctttttca tgttttttac ctcatgtttc ttagattttc ttttaggagg
     7141 gattggtaat gccgtgagac atgaacctcc ttgtgcgtct gataaaagga aatggtgccc
     7201 gttcctgaaa aggacttgtc cactgagtaa atctgacgga tgtttgcgat cgttgacttg
     7261 gaaactcgat taaaatagcg aggcaagata gactccaact catagagctt gaggcgaacc
     7321 tcataagctt ccttctggtt atgagcgtag atcttgctcc cttctgtttc aaagaagaga
     7381 atttctgaca ggtctagata atattcacct gtccccttgt aaaaagtcaa cctcggagcc
     7441 tttgactctt gcaagagccg ttgcaagtct gcaatttcat ctgtcaaagt gggggtcttg
     7501 atgacaattt cagtctcctc taaattgccg tcaatctcaa ttcgtaactt catcacatct
     7561 cctttctgac tctactatat caaagctaag ttaggaatac aagggcaaaa aagcaagtgg
     7621 tcaatttgaa gcagtaagtg gttgtaggac tagcttaact tacatgtcag aataggaaga
     7681 aaaggaatcc ccacaccaaa ccacaagcat agccaaccaa gacatccttg ggataatgaa
     7741 caccccctaa aaccctcacc aaccccagca aagctgaaaa gatcaagcat actaagccaa
     7801 taggtagact agcatgcaga caggccacgg agatgatagt tgccgaaaag acatggcgac
     7861 tgggcatcga ccgtcccgaa ctatccttat caagaagggg acagatatcc caagtttcat
     7921 aaggtctcgg gtgattgatt ttcttacgaa acagggacaa ggccacaaaa cctgatgcag
     7981 gaataagcac ataaacccca acttgctgac caagtcctag ttgcaggtaa gtggtgacta
     8041 gcaagatcag atagaccagg ggcatggcga ctgtcatcaa acgattaaaa ctgcgtaata
     8101 ggaataaaag aattggacga ctagttagtc tggagctgat atttcgatac cattcttgat
     8161 aatttttcat ttatgcttct catacttttc tagaacttgt tgagatgttt tccccttggc
     8221 aagccaatcc actagcttgt ccaagcgacg tatttcctgc atcagaggat catcaatttc
     8281 ctcaatacgg acaccgcaaa tcttccctgt aattgcaatt cgttcaggga cataatatgg
     8341 tgcttgtcta aaaaaatctc cgtaagtaac atccctatcc agacaagcaa gaacatcact
     8401 cacttcatac cctgtcaacc aactggtcac ttgatgaact gactctgcct ttccaccctt
     8461 tcgctcaact tttgcaatca aggctaagta gacagcagca aatttcatat tgtataattt
     8521 ctgactcata cttctctctt tctatgtgta ctagtataaa taagaaaaag aaggccgtca
     8581 agccttcttt tgatttattc ttctgcttca tcttctgtaa attgactatt gtacaagtca
     8641 gcgtagaagc caccttgcgc catcagttcc tcatagttgc cttgctcgat gatatttcca
     8701 tctttcatga ccaagatcaa gtctgcattt cggatggttg acaagcggtg ggcaatgaca
     8761 aagaatgtgc gtccttccat caaacggtcc atggcttttt ggatcaattc ctctgtccgt
     8821 gtgtcaacag aagaagtcgc ctcatccaaa atcaaaagcg gtgcatcctt aagaagggca
     8881 cgagcaatag tcaatagttg tttttgtcct acagacaagg tcacggtgtc atccaagatg
     8941 gtatcatagc catctggcaa ggtcataata aagtggtgaa ttcccacagc cttactagct
     9001 tccatcattc gttcatcact aatccctatt tgattataga tgagattgtc tcgaatagtt
     9061 ccttcaaaga gccaggtatc ctgcaagacc attgaaaagg catcatgcac ttctgaacgc
     9121 gtcatagcct tggtatccac accatcaatg tgaatacttc ccttatcaat ctcatagaat
     9181 ttcatcaaaa gattgacaat ggttgtctta ccagtcccag tcggcccaac aatggcaacc
     9241 ttttgaccag catgagctgt cgcagagaag tcatagtctt gaacattgac accgtccacc
     9301 agaatttctc ctgctgacac gtcgtagaaa cgtggaatca gattgaccag agttgattta
     9361 ccagaacctg ttgacccaat aaaggccact gtttgaccag tttctgcttt aaagctaaca
     9421 tgttcaataa ctgcctccga atttgccgca tagcggaagg tcacatcctt aaactcgacc
     9481 tgacctttga agttttcatc agtcagctgc acttgaacag ggttttggat agaagaatgc
     9541 aaatataaaa cttgattaat ccgcttagca gagaccatag ttcggggaag aacgatgaag
     9601 agtgctccca tgagaaggaa gcccatgaca acctacatgg cataagacat gaaaacaatc
     9661 atgtcactaa agagaggcag acgcgctatc ggagcagcgt cgttaatcac ataggcccca
     9721 atccagtaaa tcgccacact caaaccactt gaaatcccca tcatgatagg attcaaaata
     9781 gccataagac ggttgacaaa caaattcaaa cgggtcaatt catcatttac tgctgcaaat
     9841 ttttcatttt gataatcctc tgcattgtag gcacgaacga cacgaatacc tgttaaactc
     9901 tcacgagtga tactgttcag tttatctgtc agcccctgaa tcaaggactg ttttggaaag
     9961 gctagcgtca tcaaaacggt cgtcatcagg acgttgacaa tcactgccac aagtacggcc
    10021 cagagccagt attctgaatg acctaaaatc ttcccaatag cccagatagc cataattgga
    10081 ccacacgtta ccacttgcaa gcccatagta atcaacattt gaacttgagt aatgtcattg
    10141 gtagtacgcg tcaagaggct aggaattgaa aatttcttaa tctctgtctg cgagtaatcc
    10201 aaaactcggt taaaaatatc acttctcagc ctactagtat aagaagccgc cactcgggat
    10261 gcaaaaaatc caactgcaac tacggacaag aaggcaagaa agagcattcc catcatcatg
    10321 cttgccggct gccacaactc atctaaatta gttccttgac tacctagcaa atccgtaatt
    10381 ttcgagatat aggtcggcac ttccaactct agatagaccg aaaagcaagt aaagagaatg
    10441 gctagtaaaa tcatccccat tcttttctac taattctttt ggctaatttc tttattctct
    10501 cctcctattc ccttgatatt tgcctgtagt tgaccgagaa ctttctcaaa aatcagtaat
    10561 tcatcttcat caatgtcttc catcaactgc ttgtctatgc gttcaaaaaa agccttaacc
    10621 tgttgcatct gagaacgtgc tttgtccgtc agacgaacaa acttagcccg cttatcaaca
    10681 ggactcgcct ccaattccac caaaccattt tgcactatac gcttaaccag attactagca
    10741 acagacttgg taatattgag ttcctgctcg atatctttaa tcaagaccaa gtcttggttt
    10801 ttctcgcgat tatccaaaaa acgcacaacc tgaccttgcg gcccacccat aaattcaatg
    10861 ccgcaacgtt tggcttcctt ttgcaccatc aggtgaattt gatgaccaaa acgcttaaag
    10921 actaacatcg gtttacccat aatctccccc ttctaaataa aaatagttct ctggagaata
    10981 attaaatttc tatgagaact attttcttga ttaa
//
LOCUS       DAVLWA010000058        10967 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLWA010000058 DAVLWA010000000
VERSION     DAVLWA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.058"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            29..391
                     /locus_tag="VYN80_001460"
     CDS             29..391
                     /locus_tag="VYN80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541357.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGRILA
                     IIGGSLFLATLKKFKSEE"
     gene            926..1618
                     /locus_tag="VYN80_001461"
     CDS             926..1618
                     /locus_tag="VYN80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1541358.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1671..4058
                     /locus_tag="VYN80_001462"
     CDS             1671..4058
                     /locus_tag="VYN80_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1541359.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNSDYHLNVNLQMNY
                     WPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHTQATPFGWT
                     APGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFWNAFLHKDQ
                     QAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLLTEVKEKSD
                     LLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSYKGQEYIEA
                     ARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLWCSHPPFQI
                     DGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSMSWEDKKLL
                     QLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDLVQFYF"
     gene            4308..5009
                     /locus_tag="VYN80_001463"
     CDS             4308..5009
                     /locus_tag="VYN80_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541360.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5011..7719
                     /locus_tag="VYN80_001464"
     CDS             5011..7719
                     /locus_tag="VYN80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1541361.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7815..8306)
                     /gene="tpx"
                     /locus_tag="VYN80_001465"
     CDS             complement(7815..8306)
                     /gene="tpx"
                     /locus_tag="VYN80_001465"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1541362.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8429..9358)
                     /gene="psaA"
                     /locus_tag="VYN80_001466"
     CDS             complement(8429..9358)
                     /gene="psaA"
                     /locus_tag="VYN80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1541363.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9390..10238)
                     /locus_tag="VYN80_001467"
     CDS             complement(9390..10238)
                     /locus_tag="VYN80_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1541364.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10235..10957)
                     /locus_tag="VYN80_001468"
     CDS             complement(10235..10957)
                     /locus_tag="VYN80_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1541365.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3203 a         2046 c         2517 g         3201 t
ORIGIN      
        1 gaaaaaaaat aaaaaaggag attttattat gaaatcaagt ggtatcgtcg gtcttgtggg
       61 aggaatttta cttcttattt gtccttttgt cttgttggga acagcggtaa atacagctgc
      121 tacaactctt aatggaggag ctactgcagg ggctttttca ggtgttgcct tactcttgaa
      181 tgccttgaag attgcaaatc ttgttcttgg tatcattgct attgtttact ataaaggaga
      241 taagcatgta ggtgcagctc cgtctgtact aatgattgtt tctggtgaag ttagtctcat
      301 tccattctta ggatgggttg ggaggattct tgctattatc ggaggatctc tattccttgc
      361 aacattgaag aaattcaaat cagaagaata aaaggtattt tagcatgaaa agaacaaaaa
      421 agtttatcgg tataggagta gctctattat ctctttctct tctagttgca tgtggaacat
      481 aaagttcaaa gaatacttca acaagtaatg atgagaagac agtagcaaca tccaatagtt
      541 caaaagaaac aatcactttc gatacaccgg ttgtaacaga cgatgcgatt gaatcaatac
      601 gcacttatgc agattatata gatctttata aaaatatttt tgatgattat tttactaaag
      661 ctgaggaagg tttcaaaggc acaactatgg aaaataatga ctcgtttact aaactaaaag
      721 agtcaactca aaaattattc gatgcgcaga aaaaaaggtt aaataatgaa gatagaatag
      781 aaacaaccaa aaacaatgtg attgccaaac attgtcaaac agtcctttcc tttttggttt
      841 tgactagctt ttttgtgaaa aattgtgtaa aatagaatag ataaacgagg ggaaacctcg
      901 gaaaatttaa aggagaatcc atctaatggt aaaattggtt tttgctcgcc acggtgagtc
      961 tgaatggaac aaagctaacc ttttcactgg ttgggctgat gttgatttgt ctgaaaaagg
     1021 tacacaacaa gcgattgacg ctggtaaatt gatcaaagaa gctggtatcg aatttgacca
     1081 agcttacact tcagtattga aacgtgctat caaaacaact aacttggctc ttgaagcttc
     1141 tgaccaatta tgggttccag ttgaaaaatc atggcgcttg aacgaacgtc actacggtgg
     1201 tttgactggt aaaaacaaag ctgaagctgc tgaacaattt ggtgatgagc aagttcacat
     1261 ctggcgtcgt tcatacgatg tattgcctcc aaatatggac cgtgatgatg agcactcagc
     1321 tcacacagac cgtcgttatg cttcacttga cgactcagta atcccagatg ctgaaaactt
     1381 gaaagtgact ttggaacgtg ctcttccatt ctgggaagat aaaatcgctc cagctcttaa
     1441 agatggtaaa aacgtattcg taggagctca cggtaactca atccgtgccc ttgtaaaaca
     1501 catcaaaggt ttgtcagatg atgagatcat ggacgtggaa atccctaact tcccaccatt
     1561 ggtattcgaa ttcgatgaaa aattgaacgt tgtttctgaa tactaccttg gaaaataaaa
     1621 aattgtaagc ctaggattga tttctaggct ttttatgtta gtatggaagt atgataagga
     1681 ataaaaaaca agattatgta ctggcctaca agcaaccagc ttcaaccact tacatgggtt
     1741 gggaagaaga agctttaccg ataggtaatg gttctttagg agcaaaagta tttggcctta
     1801 tagggtctga acggattcaa tttaatgaaa aaagtctctg gtctggaggt ccacttcctg
     1861 atagttcaga ttatcagggt ggaaatcttc aggatcagta tgttttttta gctgagattc
     1921 ggcaggcttt ggagaagaga gattacaatc tggctaagga actggctgag cagcacctaa
     1981 ttgggccaaa aacgagtcaa tatgggacct atctgtcttt tggggatatt cacattgagt
     2041 tcagccagca aggtacgact ttgtctcagg tgacggacta tcagagacag ttgaatatta
     2101 gtaaggcact tgcgacgact tcttatgtct ataagggaac gcgatttgaa cgtgaagctt
     2161 ttgcgagttt tccagatgat ctcttggttc aatgttttac taaggaaggg ttggaaactc
     2221 tagattttac tatagaacta tccttgacct gtgatttggc ttctgatgga aagtatgagc
     2281 aggaaaaatc tgattacaag gagtgtaagt tggatattac tgattctcat atcttgatga
     2341 agggaagagt taaggataat gatctgcggt ttgctagtta tctagcttgg gaaacggatg
     2401 gagatattag agtttggtca gatagggttc agatatcagg agccagttat gccaatctct
     2461 tcttggccgc taagacggat tttgcccaaa atcctgctag caattatcgc aagaaactag
     2521 atttagagca acaggtgata gacttggtgg acacagctaa agaaaagggc tatacccaat
     2581 tgaaatcaag gcatatcgag gactaccaag ccttattcca gcgtgttcaa ttggatttgg
     2641 aagctgatgt tgacgcatcc actacagatg atttgttaaa aaattataag ccacaagaag
     2701 ggcaggcttt ggaggagctg ttcttccagt atggacggta tttattgatt agttcgtcca
     2761 gagactgccc agatgctcta ccagctaacc tacagggagt ctggaattcg gactatcact
     2821 taaatgtcaa tctgcagatg aattattggc cagcctatgt taccaatctc ctagaggcgg
     2881 tctttccggt catcaactat gtagatgatt tgcgtgtcta tggtcgtcta gcggctgtaa
     2941 agtatgcagg aatcgtctct caaaaaggtg aggaaaatgg ttggttggtt catactcaag
     3001 cgactccctt tggttggacg gcacctggtt gggattacta ttggggttgg tcaccagctg
     3061 ccaatgcgtg gatgatgcaa accgtttatg aagcctattc attttatagg gaccaagact
     3121 atctcaggga gaaaatttat cccatgttga gggaaacggt tcgtttttgg aatgcctttt
     3181 tacataagga tcagcaggcg cagcgttggg tgtcttctcc gtcttattct ccagaacatg
     3241 ggccgatttc gattggcaat acctatgacc aatctctgat ttggcagtta tttcatgatt
     3301 ttattcaggc tgctcaggaa ttgggactgg atgaggactt gttgactgag gttaaggaga
     3361 agtctgattt actaaatcct ttgcaaatca ctcaatctgg tcgaatcagg gagtggtatg
     3421 aggaggaaga gcagtatttt caaaatgaga aagtggaggc ccagcatcgg cacgcttccc
     3481 atctagtggg actctatcct ggcaatctct ttagctacaa gggacaagag tatattgaag
     3541 cggcgcgtgc tagcctcaat gatcgtggag atggcggcac aggctggtcc gaggctaata
     3601 agatcaatct ctgggcgcgt ttgggagatg gcaatcgagc ccataaatta ttggcagagc
     3661 agttaaagac atccaccttg caaaatcttt ggtgtagcca tcctcctttt cagatagatg
     3721 gtaattttgg tgctactagt ggcatggcag aaatgttact ccagtctcat gcagcttatc
     3781 tggtacctct agctgcccta cctgatgctt ggtcaacagg ttctgtttca ggcttaatgg
     3841 cacgtggaca ttttgaagtg agcatgagct gggaagataa aaaactctta cagttgacca
     3901 ttttatcaag gagtggagga gatttgcgag tttcttatcc agatattgag aagagtgtga
     3961 ttaaaatgaa tcaagaaaaa ataaaagcga aatgcatggg gaaagattgt atttcggtgg
     4021 caacagcaga aggtgatctt gttcaatttt atttttaaga agatgttata aggcagtaat
     4081 ttgaaactgc cttttaataa ggatttaaga atataagcag ttttcaacta gttgaaaaaa
     4141 cgttataatg ataataggaa gtaatactca atgaaaatca aagagcacaa actaggaagc
     4201 tagccgcagg ttgctcaaaa cagtgttttg aggttgcaga tggaagctga cgtggtttga
     4261 agagagattt tcgaggagta taatttgttt gatagagggt gggtctgatg gcttatattg
     4321 agatgaaaca ctgttacaag cgttatcagg ttggggacac ggagattgtg gccaatcgtg
     4381 atgtgaattt tgagattgaa aagggggagc tggttattat ccttggtgct tcaggtgcag
     4441 gcaagtcaac agttcttaac cttcttgggg gaatggatac caatgatgaa ggggaaatct
     4501 ggattgatgg tgttaatatt gcggattata gttcccacca gcgcaccaat taccgtagaa
     4561 atgatgtggg gtttgttttt cagttttata atctagtttc taatctgaca gctaaggaaa
     4621 atgtggaact ggcttctgaa attgtgacag atgccttgaa ttctgatcag gtcttgacag
     4681 atgtaggtct ggctcatcgt ctcaataact ttccagccca gctttctgga ggggagcaac
     4741 agcgagtctc cattgcacgc gcggtagcca aaaatcctaa aattcttctt tgtgatgaac
     4801 ctactggagc cttggattat cagacgggca agcaggtttt gaaaattctc caagacatgt
     4861 ctcgtcaaaa gggagcgacg gtgatcatcg tgactcataa tggagctttg gcgcccattg
     4921 ctgatcgcgt gattcatatg cacgatgcca gtgtcaagga tgtggtgctc aaccagcatc
     4981 ctcaggatat tgacagtttg gagtactagc atgatcaagc gaaaaactta ttggaaggac
     5041 ttaattcagt ccttcacagg ctccaagggg cgttttttat ccatcttgat cctgatgatg
     5101 ttgggatctc tagccttagt aggcctcaaa gtaaccagtc ccaacatgga ggcgacagct
     5161 aatgcttatt taacaactgc tcaaaccttg gatttggcag tcatgtctaa ctatggcttg
     5221 gatcaagcag accaagaaga actaaaacag acggagggcg cagaggtcga gtttggctat
     5281 ttgacagatg tgactatgga taatgggcag gatgccattc gactgtactc caaaccagag
     5341 cgaatttcaa cctttcagct aagaaaggga cgacttcctc agtcagacaa ggaaatcgct
     5401 ttggccactc atttgcaagg ccaatacagc gtgggacagg agattagttt taaagaaaaa
     5461 gaagagggtc attcctcttt aaaagaccat acttatacca ttactggttt tgtggattcg
     5521 gctgaaatcc tctcccagcg agatatgggc tacgcaggaa gtggaagtgg gactctgaca
     5581 gcctatgggg tgattttacc tagtcagttt gatcagaaag tctacaatat agctcgtttg
     5641 aaatatcaag atttagcagg tttaaatgcc ttttcatcag cttatgaaga aaaatccaag
     5701 caacatcagg aagagcttga acaaatttta tcagataatg gcaaggtacg tctgcaactt
     5761 ttgaaaaaag aaggacaaga gtctctagac aaggggcaag agacccttga caaggctcag
     5821 actaatttgc aggaaggcaa gcgtcgttta gcagctgctc aagctcgtat acaggctcaa
     5881 gaaagtcaac tagccttgtt tcctcaagtt cagagagagc aggctagtgc tcaacttacc
     5941 caagccaagc aggaattggg caaggaagag gccaaactaa agcaagctga acaaaatcta
     6001 gcccaagaaa aggaaaaatt agaaaaacat cagcaagtct tggacgattt ggcggagcca
     6061 aggtatcagg tctataatcg tcagaccatg ccaggtggtc agggctatct tatgtatagc
     6121 aatgcttcat ccagtattcg agcagtgggc aatatctttc ctgtggtact ttatgccgta
     6181 gcagccatgg tgacctttac gaccatgact cgctttgtag acgaagagcg aactcatgca
     6241 gggattttta aggccttggg ttaccgtagt aaggatatta tcaccaagtt tctcctttat
     6301 ggactagtag ctgggactgt cggaacggct ctaggtagta tacttggtca ttatttgcta
     6361 gccagtgtaa tttcaagtgt cattacaaaa ggcatggtgg tgggagaaac ccagattcag
     6421 ttctattgga cctatagctt actagctctt gtcttgagct tgttggcgag tgtgttacca
     6481 gcctatctgg tggcttggag ggaacttcat gccgaagcag cccagcttct acttcctaaa
     6541 cctcctgtca aaggagctaa aatcttattg gagcgtatcg gttttatctg gcgtcgtctc
     6601 agttttactc ataaggtaac agcccgcaac atctttcgtt ataagcagag aatgttgatg
     6661 acaatctttg gtgtggcagg ttctgtagct ctgctctttg caggtttggg aatccaatct
     6721 tctgtagcag gagttccgtc taaacagttt caacaaatcc aacagtatca gatgcttgtc
     6781 tctgaaaatc ctagtgcgac caatcaggac aaggtagagc tagcagaagt gttgaaaggg
     6841 caggagatac tagcctacca gaaaatctat tctaaaacgc tagacaagga tttcaaaggc
     6901 aaggctggtc ttcaaaacat tactcttatg atgatagaga aggaagattt gactcccttt
     6961 atccatcttc aacatcatca gcaggagctg acattaaaag atggcatcgt tattacagct
     7021 aaactcgccc agctggcagg tgtcaaggtt gggcagactt tagaaattga aggtaaggaa
     7081 ctaaaggtcg ctgctattac tgagaactac gttggtcact ttatttatat gagtcaggct
     7141 agctatgagc aactttacgg acagctaccc caagccaaca cttatctggt ctcattaagg
     7201 gataccagtg caactagtat cgaaagtcag gcgggcttgc ttatgaatca atctgcggtg
     7261 tccagcgttg tccaaaatgc ttcagccatt cgactcttcg actctatcgc tagctcactc
     7321 aatcagacca tgaccatctt ggtcatcgta tcggttctat tagctattgt catcctttac
     7381 aatctgacca atatcaacgt agctgagaga atccgtgaac tctccactat caaggttctc
     7441 ggttttcata ataatgaagt caccctctac atttaccgtg agacgattgt gctgtccctt
     7501 gtgggaatcg tacttggtct ggtagctggt ttctatttac accaattttt gattcaaatg
     7561 atttcgcctg cgactattct cttttatccg caggtaggct gggaagtcta tgtaatccca
     7621 gtggcagcag taagcatcat tttgaccttg cttggtttct tcgtcaatta ttatctgaga
     7681 aaggttgaca tgttagaagc cctgaaatct gtagagtaag gtagttattt ttagctgatt
     7741 gaacttctat ttactaatat tcaaaaatcc tccgtttcaa agagcaggga actctttgtg
     7801 acagaggatt ttttctatag ggctttagca gctgcaattg cggcttcgaa gtttggctca
     7861 gaattgatat tatccacgta ttcaacgtag cgaatcgtat tgtcagtatc gaggacaaag
     7921 actgcgcgtg ctaataggtg ccattcgttg atcaagaggg cataatcgtg cccgaaagaa
     7981 tggtcaaagt agtctgaaag cataatggca ttgtcaaggc cttcagcacc gcaccaacgt
     8041 ttttgagcaa aaggtaggtc cattgaaaca gtcaatacga ccgtgttgtc cagtccagcc
     8101 aattcttcat taaaacgacg tgtttgagtt gagcagatgc ctgtatcgat agaaggaacg
     8161 acactcaaga cttttttctt gccatcaaaa tcagccagag attttttaga aagatctgtt
     8221 gtagtaagag aaaaatcaag cgccttgtcg ccgacttgta gttgtttacc tgtaaagctc
     8281 acaggatttc cgagaaaagt taccatagga tactccaatc ttttttcttc cattgtatct
     8341 gaaacagtca gaattttcca atgatttgac cggaaatgtg ggcatagaaa aaacgccagc
     8401 tcatatgaga atgacgtttt tcagaggctt attttgccaa tccttcagca atcttgtcaa
     8461 ggttgtattt catcatgctg tagtagctgt cgccttcttt accttgttct gcgatagagt
     8521 cagtaaagat ttgtgcgtag attgggatgt ttgtgtcttg agaaacggtt ttcattggac
     8581 ggtcatccac acttgattct acaaagagtg atggaacttt tgtttggcga agtttttcaa
     8641 ccaaggtctt gatttgttca ggagttcctt cttcttcagt attgatttcc cagatgtagg
     8701 cacttggaac accataggct ttagagaagt atttgaatgc tccttcgctg gttacaatga
     8761 gtttcttttc agcagggatc ttattaaatt tatccttact ttctttatca agtttgtcta
     8821 acttatcagt atattctttg agattttttt catagaattc tttattgtta gggtctttgg
     8881 cgctcaattg tttggcgata tttttagcaa aaataatacc gttttcaagg ttaagccaag
     8941 cgtgtgggtc ttcttttcct ttttcatttt gaccttcaag gtagataaca tcaacgccgt
     9001 cgctgactgc gaagtagtct ttgttttcag ttttcttggc attttctacc aattttgtaa
     9061 accaagcatt gccacctgtt tcaaggttga taccgttata gaaaatcaaa tcagcctcag
     9121 aagttttctt aacgtcttca ggaagtggtt cgtattcgtg tgggtcttgc ccaatcggaa
     9181 cgatactatg aaggtcaatt ttgtcaccag caatattttt agtaatatca gcgatgattg
     9241 agtttgtagc aacaactttt agtttttgac cagaagttgt atcttttttt ccgctagcac
     9301 atgctacaag aatgattgca gaaagaaaga gaacgagtaa tgtacctaat tttttcatta
     9361 gatcctccaa tttattaggg ctttgcccct tattttaaca aatgtttatt tttcagtttc
     9421 aaatatcgtt gtttgggagc gataaagaag ctaatgagaa agaaactagc agctgtaagc
     9481 acgatactag aacctgccgc aacattaaaa ctatagccaa taaagagtcc caaaactgaa
     9541 gcagtagctc cgaaggttga ggaaaggaaa atcatgcttt tcagactatt agcatacaga
     9601 taagcagttg cagctggggt aatcagcatg gctacaatca ggatagttcc gacactttgc
     9661 atggctgtca cagacacgag agtcaggagt accatgagaa ggtagtgata gaaattgaca
     9721 ggcattccca tggctttagc caagagttca tcaaaggaag ttatcaagag ttgcttgaag
     9781 aaaatccaga ttaacaagag gatggctgcc cccacaccca tagtaataaa catatccgta
     9841 tcttggacgg ccaggatatt accaaaaagg atatggaaaa ggtcagttga acttttagcg
     9901 acaccaatca agatgatacc gagggctaag aaagaagaaa aggtaatgcc gatggcggta
     9961 tcgcttttga taatcgagtt tcctttgatg taggtaatga tgatggcagc tagcaatcca
    10021 aagacaatgg ctccgataaa gaagtcaagg cccaagatga aggagagggc tacacctggt
    10081 aagacagcat gtgaaatggc atctcccatg agtgacatcc cacgtagaat aatgaaacat
    10141 cccacggctc cagctacaat cccgacgaca atagctgtta tcaaggcatt ttgtaggaaa
    10201 tggaattttt gcaatccatc gataaattct gcaatcatag gtcacctcca ttgaaaaaga
    10261 gttgattacc gtaagcttct tttagattgg tttcggtaaa agtttctttt gttggaccaa
    10321 aggcaatcac ttctcgattg acaagtaaga cttgatcgaa gtagtgggga atcttgctga
    10381 ggtcgtggtg aacgatgaga accgtcttcc cagctttttt caaatctctc agcgtattca
    10441 tgatgatttc ctcactgaca gagtcaatcc cagcaaaggg ttcatccaag aggatatagt
    10501 cggcttcctg caccaaacat ctggcaatca agacccgctg gaattgacct ccagacagtt
    10561 gactaatttg acgttcagcg tagtcagcta ggccgacgat ttcaagggcc tcttgcactt
    10621 tcttccaatg tttagccttt aaacttcgaa agagaggaat agagggaaat agtcctaacg
    10681 agacgcattc cttgaccttg atgggaaagt tgtagtcgat attgattttt tgttcgacat
    10741 aggcaattcg gtgtaaggat tttttaactt ccttgtcatc gagaaatgcc tgaccttgat
    10801 gtgggataat tcccagcata ccttttaata gtgttgattt cccagcgccg tttggaccaa
    10861 tgatgccggt aattgttggt ccatggagca ctagtgaaat atccttaagt gccaacgttt
    10921 ctttgtagga gacactgagg ttttcgatac gtatcataaa cttgtat
//
LOCUS       DAVLWA010000059        10721 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLWA010000059 DAVLWA010000000
VERSION     DAVLWA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.059"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            75..572
                     /locus_tag="VYN80_001469"
     CDS             75..572
                     /locus_tag="VYN80_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1541366.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            572..2227
                     /gene="dnaX"
                     /locus_tag="VYN80_001470"
     CDS             572..2227
                     /gene="dnaX"
                     /locus_tag="VYN80_001470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1541367.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2255..2449
                     /locus_tag="VYN80_001471"
     CDS             2255..2449
                     /locus_tag="VYN80_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1541368.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2599..3369
                     /gene="sufC"
                     /locus_tag="VYN80_001472"
     CDS             2599..3369
                     /gene="sufC"
                     /locus_tag="VYN80_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1541369.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3397..4653
                     /gene="sufD"
                     /locus_tag="VYN80_001473"
     CDS             3397..4653
                     /gene="sufD"
                     /locus_tag="VYN80_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1541370.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     NVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAIDRL
                     GENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSSGR
                     QVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVLML
                     SDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGFLG
                     SVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4664..5890
                     /locus_tag="VYN80_001474"
     CDS             4664..5890
                     /locus_tag="VYN80_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1541371.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPLKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5877..6317
                     /locus_tag="VYN80_001475"
     CDS             5877..6317
                     /locus_tag="VYN80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1541372.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6371..7783
                     /gene="sufB"
                     /locus_tag="VYN80_001476"
     CDS             6371..7783
                     /gene="sufB"
                     /locus_tag="VYN80_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1541373.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFGDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8014..9255)
                     /locus_tag="VYN80_001477"
     CDS             complement(8014..9255)
                     /locus_tag="VYN80_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1541374.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9358..10467
                     /locus_tag="VYN80_001478"
     CDS             9358..10467
                     /locus_tag="VYN80_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1541375.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3180 a         1958 c         2330 g         3253 t
ORIGIN      
        1 gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg ctcctttttt
       61 gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag
      121 aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg
      181 ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa
      241 aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca
      301 agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga
      361 cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga
      421 tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg
      481 atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacaacat
      541 gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag
      601 aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc
      661 ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa
      721 aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga
      781 accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat
      841 tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc
      901 tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct
      961 gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt
     1021 ctttattttg gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca
     1081 acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt
     1141 agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca ggcgggcgga
     1201 aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa
     1261 tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga
     1321 tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct
     1381 tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag
     1441 agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga
     1501 aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgagttt
     1561 agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt
     1621 ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct
     1681 gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gtaggtgcgg ttcctaaaca
     1741 agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa
     1801 taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct
     1861 aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc
     1921 tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga
     1981 gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa
     2041 tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg
     2101 gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt
     2161 agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga aggtagagga
     2221 agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat
     2281 ggcgctgttt acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta
     2341 tattatggca gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat
     2401 gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc
     2461 cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga
     2521 atttttgctc tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg
     2581 aaaaagaaag gaactatcat gtcagtatta gagatcaaag atcttcacgt tgagattgaa
     2641 ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc
     2701 atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac
     2761 tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg
     2821 gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga
     2881 attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga tgatgagaag
     2941 atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct taacatgaaa
     3001 gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat
     3061 gaaattcttc aacttttgat gttggagcca acatttgctc ttttggacga gattgactca
     3121 ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa
     3181 ggttttggtg ctatgatcat tactcactac caacgtcttt tgaactatat cacacctgat
     3241 gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg
     3301 cgtttggaac gtgaaggata cgcaaaatta gctgaagaac ttggctacga ctacaaggaa
     3361 gaattgtaat tccctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt
     3421 ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac
     3481 aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg
     3541 ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta
     3601 gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt
     3661 gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca
     3721 gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct
     3781 taccacacag cttactttaa cagtggtgct gtactctata ttccagataa tgtagaaatc
     3841 acagagccaa ttgaaggaat tttctaccaa gatagcaatg tgccgtttaa caagcatatt
     3901 atgattatcg ctggtaaaaa ttctaagatt aattatctgg agcgtttaga gtcacgcggt
     3961 gaaggaagtg ccaaagcaac tgctaatatc acagtagaag tgattgcacg ttcaggtgcg
     4021 caagtcaagt ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta cattagccgt
     4081 cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga
     4141 aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag
     4201 gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat
     4261 ggctgcaact ctatcggaaa tatcctacaa catggggtta tccttgaaaa agcaactttg
     4321 actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc ccaacaagag
     4381 agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt
     4441 gatgaaaatg acgtcactgc aggacatgca gcctctattg gtcaggtaga tccagaagat
     4501 atgtactacc tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt
     4561 ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt
     4621 gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc
     4681 gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct
     4741 ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctatta acagttacta
     4801 tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc
     4861 ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga
     4921 agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga
     4981 aattctcact gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat
     5041 tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga
     5101 cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc
     5161 cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt
     5221 agcccaccaa gttggggcaa tcatggtagt ggatggtgct caatctacac ctcatatgaa
     5281 gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg
     5341 tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt
     5401 agaatttggc ggcgagatga ttgattttgt ctacgagcaa tctgctagtt ggaaggaatt
     5461 gcctttgaaa tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc
     5521 agttgattat ctggaaaaga ttggtatgga tgccgttgaa gctcatgaac aggaattgat
     5581 tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga
     5641 tttggctcaa cgttcgggtg ttattgcctt taacctaggt gatctccatc ctcacgatct
     5701 tgcgacggct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc
     5761 cttgcttcag tatttggaag tcccagcaac agctcgtgca agtttttata tctataatac
     5821 caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg
     5881 cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac
     5941 atcaccaagg gaagttagaa gacgctgagc aaatcagtct caacaatccg acttgtggag
     6001 atgtcatcaa tctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc
     6061 taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag
     6121 gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc
     6181 aaaaagatga gcgtcaagac caacttggag acgcggcatt cttgtcaggt gttgccaaat
     6241 tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa
     6301 atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag
     6361 aaaggatatt atggctgaag aaagagtaga accaaaacca attgaccttg gtgaatataa
     6421 atttggtttc catgacgatg tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg
     6481 tgttattcgt gaattatctg ctgctaaggg tgagcctgag tggatgttgg agttccgttt
     6541 gaagtcttat gaaaccttca aaaaaatgcc catgcaaact tggggagcag acttgtcaga
     6601 gattgacttt ggtgacttaa tctactacca aaaaccatct gacaaaccag cccgttcttg
     6661 ggatgatgta cctgaaaaga ttaaagaaac ctttgaacgt atcgggattc cagaagctga
     6721 acgtgcttat ttagcagggg cttctgccca gtacgagtca gaagtggttt accacaacat
     6781 gaaggaagag ttccaaaaat taggtattat ctttacagat acagattccg cactcaagga
     6841 atacccagac ttatttaatc aatactttgc gaagttggta ccgccgacag ataacaagtt
     6901 ggcagccctc aactcagcag tatggtcggg tggaactttt atctacgtgc caaaaggtgt
     6961 caaggtagat attccacttc aaacttattt ccgtatcaat aacgaaaata taggtcagtt
     7021 cgaacgtacc ttgattatcg ttgatgaggg agcaagcgtc cactacgtag aaggatgtac
     7081 agcaccaaca tattcaagca atagcttaca cgctgccatt gtagaaattt ttgctttgga
     7141 cggagcttat atgcgttata caactatcca aaactggtct gataacgtct ataacttggt
     7201 aacaaagcgt gctaaggctc aaaaggatgc cactgttgag tggattgatg gaaacttggg
     7261 tgccaaaacg actatgaaat atccatctgt ttaccttgat ggagaaggag cgcgtggtac
     7321 catgctctct atcgcctttg ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat
     7381 tcacaatgct ccacatacca gctcgtctat tgtgtctaaa tccatcgcta aaggtggagg
     7441 aaaggttgac taccgtggac aagtcacctt taacaagaac tctaagaaat ctgtttccca
     7501 cattgaatgt gataccatta tcatggatga cttgtcagca tcagatacta ttccatttaa
     7561 tgaaattcac aactcgcaag tggctttgga acacgaagcc aaagtatcta agatttcaga
     7621 agagcaattg tattatctca tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat
     7681 tgtcatggga tttgtagaac cctttacaaa agaacttcca atggaatacg cagttgagct
     7741 gaaccgcttg attagctatg aaatggaggg atcagttgga taaaatttga ttttatactc
     7801 ttcgaaaatc tcttcaaacc acgtcagcat cgccttaccg tatgtatggt tactgacttc
     7861 gtcagtttca tctacaacct caaaacagtg ttttgagcaa cctgaggcta gcttcctagt
     7921 ttgttctttg attttgagta ttagatttac tcaaaatcaa ggattttgaa gatgaacttg
     7981 tatcaaaaaa tcgcggttta aaatcgcgat tttttataat ttctcgttaa caaagcggac
     8041 aaactgattc caccaaactt ttaagaagaa ggctttttca attttcttgt ctgctaccat
     8101 ttcgaaacta gggcgctctg tggtgatgta accttgacca atcaagtcct tgtcttcata
     8161 agtcaaatgg ccaaccactg ttccagcttc aagtggtgct gggattgctt tggaatcagg
     8221 tgtgaattga acagattggg aagattgatt cccaacacgt tcgattagat agatatcctc
     8281 tggagccact gcagttactg tatcttcttt tccatcttgt acaggggctt tgctatcttg
     8341 ataggcatcg ccttgttgaa cgattttgcg aagtgtaaat gtagaagaaa tataatccat
     8401 tagggaagat gtagctgtaa atcgagcgta aggattattg tcttgatgat ctgcatttaa
     8461 aacaactgtg atgactctca tgcctttttc gacagtagta ccaacaaaag actctccagc
     8521 cttatctgtt gttcctgttt ttagcccatc aaaaccacca cggtaagcag gcataccttc
     8581 taacatgtag ttggttgaag tgattgtcat cccagcaaaa gtagaagaag gttttttggt
     8641 gatttctaag acttgtgggt attttttgat gaggttgcga gcaacgatag cgacatcata
     8701 agcactaagc ttattttcct catctttttt agaacctggg taaatgttat cccctagagt
     8761 ttcattgtta agacctgtcg tattgacaac agtggcatcc tgaattcccc attccaagag
     8821 ttttgcccgc atcatatcga cgaaatcttt ttctgagcca gcaattttct cagctagggc
     8881 aatagcggcg ctgttggcac tagataccag agttgcttca agcaactctt cgacagtata
     8941 attacgggcc tccataggaa tattactggc ttcagaattt gtcgtcaatt gataaggata
     9001 atcagaaata tctacaggag tggagagggt aatacttccg ttttccaaag cttcatagac
     9061 cagataaaca gtaatcaatt ttgttatgga agcaatttcg acaggttgcg ttgcatcctt
     9121 ctcatagaga attttaccag tatttgcctc aacagcaatc gcatgtttag cggcaatggt
     9181 aaaatcttga gcaacagcag tagaagcacc ccctaaaaga gagacagtta acaaagttaa
     9241 aaatattttt ttcatagtag tcttattcta tcataaagaa aaaaaatatt cttgctttaa
     9301 taattcatct gttaagcttt ttgaaaatat ggtaaaataa agtgagggag gtaactcatg
     9361 tttcgtagaa ataaattatt tttttggacc acagaaattt tactcttaac catcatcttt
     9421 tacctatgga gacagatggg gtctttgatt aacccttttg ttagcgtgct taatacaatt
     9481 atgattccat ttttattagg aggctttctt tattatttga caaaccctat tgttactttc
     9541 ttaaataaag tctgtaaact caatcgtttg cttggtattt taattacctt gtgtactttg
     9601 gtctggggaa tggtcatagg tgttgtctat ctcttaccta ttttgattaa tcagttatct
     9661 agtttgatta tatctagtca aactatttat agtcgagtac aagacttaat catagactta
     9721 tctaattatc ctgcgctcca gaatttggat gtagaagcta caattcagca gttaaactta
     9781 tcctatgtgg atattcttca aaatatccta aatagcgtat caaatagtgt ggggagcgtc
     9841 ttgtcagctc ttatcagtac tgttttgatt ttgattatga ctccagtttt tttggtttat
     9901 ttcttattag atggacataa attcttgccc atgcttgaaa gaacgattct aaagagggat
     9961 cgcttgcata ttgcaggctt attaaagaat ttaaatgcga cgattgctcg ctatattagt
    10021 ggagtttcga ttgacgcaat cattataggt tgtttggctt atattggcta tagtattatt
    10081 ggtttaaaat atgctttagt ttttgccatt ttttctggtg tagccaattt aattccttat
    10141 gtggggccaa gtattggttt gattcctatg atcatcgcaa atatattcac tgatccccat
    10201 agactgctga ttgcagtgat ttatatgctt gttgttcagc aggtagatgg caatatctta
    10261 tatcctcgaa tcgtaggaag tgttatgaag gttcatccaa tcacgatttt agttttactt
    10321 ttgttgtcaa gcaatatcta tggtgtagtt ggaatgattg tcgcagtgcc aacctattct
    10381 atcttgaaag aaatttctaa gttcttatcc cgtttgtatg aaaatcataa aataatgaaa
    10441 gaacgagaaa gagaattagc taagtaaaag tcaggagaac cctgattttt ctttactgga
    10501 agtggccttt agattagaag actgaaaata agttaaagtc ttaaactaat tttcacagct
    10561 aagaatagta gaagttaatc tgataaaaat cgaaaaaacc agtggaattc tgtgtcaggg
    10621 taagttccac tggttttcat agtctattaa agttcgaatg aaacctattt atagtagatt
    10681 gaaactagaa tagtacacct ctaattctaa aacattgtta g
//
LOCUS       DAVLWA010000060        10624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLWA010000060 DAVLWA010000000
VERSION     DAVLWA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.060"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            92..1234
                     /locus_tag="VYN80_001479"
     CDS             92..1234
                     /locus_tag="VYN80_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1541376.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            1243..2457
                     /gene="thiI"
                     /locus_tag="VYN80_001480"
     CDS             1243..2457
                     /gene="thiI"
                     /locus_tag="VYN80_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1541377.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            2592..3416
                     /locus_tag="VYN80_001481"
     CDS             2592..3416
                     /locus_tag="VYN80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1541378.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMLYFLGNLYL"
     gene            3429..4595
                     /locus_tag="VYN80_001482"
     CDS             3429..4595
                     /locus_tag="VYN80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1541379.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(5086..6756)
                     /locus_tag="VYN80_001483"
     CDS             complement(5086..6756)
                     /locus_tag="VYN80_001483"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1541380.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            6986..7675
                     /gene="coaB"
                     /locus_tag="VYN80_001484"
     CDS             6986..7675
                     /gene="coaB"
                     /locus_tag="VYN80_001484"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1541381.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            7687..8238
                     /gene="coaC"
                     /locus_tag="VYN80_001485"
     CDS             7687..8238
                     /gene="coaC"
                     /locus_tag="VYN80_001485"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1541382.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            8222..8785
                     /locus_tag="VYN80_001486"
     CDS             8222..8785
                     /locus_tag="VYN80_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1541383.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            9067..9594
                     /locus_tag="VYN80_001487"
     CDS             9067..9594
                     /locus_tag="VYN80_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1541384.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            9606..10121
                     /locus_tag="VYN80_001488"
     CDS             9606..10121
                     /locus_tag="VYN80_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1541385.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(10118..10585)
                     /locus_tag="VYN80_001489"
     CDS             complement(10118..10585)
                     /locus_tag="VYN80_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1541386.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT         3167 a         2229 c         2128 g         3100 t
ORIGIN      
        1 aaaatcttaa aaaaagagaa attgcccttt cttgtcgcag gctccgtttc atgatacaat
       61 agaaggagtg attttagaaa gcgtgagaaa catgatttac tttgataatt cggcgacgac
      121 caggccctat ccagaagccc ttgaaaccta tatgcaggtg gcttcaaaaa ttttaggaaa
      181 tccatctagt ctccatcgtt tgggagacca ggcaacacga attttagatg cttcccgtca
      241 acagattgca gatttaatcg gtaagaaaag tgatgaaatc ttctttacat ctggtgggac
      301 agaaggggat aactggatca tcaagggagt agcatttgaa aaagcccagt ttggcaagca
      361 catcatcgta tcagccattg aacatccagc agttaaggaa tcagccctct ggttgaaagc
      421 tcaaggtttt gaagtggatt ttgctccagt tgataacaaa ggatttgtgg atgttgaggc
      481 gctagaagat ttgataagac ctgatacgac cctcgtttcc atcatggctg tgaacaatga
      541 aatcggctct atccaaccta ttgaggctat ttcagaattc ttggcagaca agccaactat
      601 ttccttccac gttgatgcgg ttcaggcgct tgccaagatt ccgactgaaa agtatctgac
      661 agaacgagtg gatttcgcga ctttctctgg tcacaagttc cacggaatcc gtggtgttgg
      721 ttttatctat atcaagtctg gcaagaagat tacgcctctt ttaacaggtg gtggtcagga
      781 acgtgattac cgttcgacaa ctgaaaatgt ggcaggaatt gcagcgaccg ctaaggctct
      841 ccgtttatct atggaaaagc tagatatctt taggagcaag actgggcaga tgaaggcagt
      901 gattcgtcaa gcacttctgg actatccgga tatatttgtc ttttcagatg aggaagactt
      961 cgcccctcat attctgactt ttggaatcaa gggtgttcgt ggtgaggtca ttgttcacgc
     1021 ctttgaagac tatgatattt tcatctcaac gacctcggct tgttcgtcca aggctgggaa
     1081 accagccgga accctgattg ctatgggagt ggacaaggat aaggctcagt cagctgtgcg
     1141 tcttagtcta gaccttgaaa atgatatgag tcaggtcgag cagttcttga ccaagctaaa
     1201 attaatttac aatcaaacta gaaaagtaag ataggagcat tcatgcagta ttcagaaatt
     1261 atgattcgct acggtgagct gtcaaccaag ggcaaaaatc gtatgcgttt catcaataaa
     1321 cttcgcaata atatttcaga cgttttgtct atctatcccc aagttaaggt aacagcagat
     1381 cgcgaccgtg cccacgctta cctcaatgga gcagattaca cagcagtagc agaatcgctc
     1441 aaacaagtat ttggaattca aaacttttcc cctgtttata aggttgaaaa atctgtagaa
     1501 gttctgaagt ctgctgtcca agagattatg caggacatct acaaggaagg catgaccttt
     1561 aaaatctcta gcaagcgtag cgaccacacc tttgaattgg acagtcgtga actcaaccaa
     1621 acgcttggag gggctgtatt cgaagccatt ccaaacgtac aagctcaaat gaaaagtcct
     1681 gacatcaatc ttcaggtgga gatacgtgaa gaagcagcct atctttctta tgaaaccgtt
     1741 cgaggggcag gtggattacc agtgggaact tctggtaagg gcatgctcat gttgtcaggg
     1801 ggaatcgatt cccctgtagc tggctatcta gcgcttaaac gaggggtaga tatagaggca
     1861 gttcactttg ccagcccacc ttacacgagt cctggtgctc ttaaaaaagc ccaagatttg
     1921 acccgtaaat taaccaaatt tgggggaaat atccagttta tcgaagttcc ttttacagag
     1981 attcaagagg aaatcaaagc caaagcgcca gaagcctacc ttatgacctt gacgcgtcgt
     2041 tttatgatgc gtattacgga ccgtattcgt gaggtacgaa atggtttagt tatcatcaat
     2101 ggggaaagtc taggtcaagt agccagccaa acccttgaaa gtatgaaggc tatcaatgct
     2161 gttaccaaca ctcccatcat tcgtcctgtg gttaccatgg acaagttgga aatcattgac
     2221 atcgcccagg aaatcgatac ctttgacatt tcaatccaac cgtttgaaga ctgttgtact
     2281 atttttgcac cagatcgtcc aaaaacaaat cctaaaatta agaatgcgga gcagtacgaa
     2341 gcgcgtatgg atgttgaagg cttggttgag cgagcagtgg ctggaatcat gattactgaa
     2401 atcacacctc aagccgaaaa agatgaagtt gatgacttga ttgacaatct gctctaattc
     2461 agaaaatcca aaagaatagc gaaaatcagt aaaaaaagtt agttttttct ctaaaaacag
     2521 gtaaaaaact aacttttttt atttttatga tataatgata taaaattttg aatatagaga
     2581 gttttctgac aatgaatcaa tcctactttt atctaaaaat gaaagaacac aaactcaagg
     2641 ttccttatac aggtaaggag cgccgtgtac gtattcttct tcctaaagat tatgagaaag
     2701 atacagaccg ttcctatcct gttgtatact ttcatgacgg gcaaaatgtt tttaatagca
     2761 aagagtcttt cattggacat tcatggaaga ttatcccagc tatcaaacga aatccggata
     2821 tcagtcgcat gattgtcgtt gctattgaca atgatggtat ggggcggatg aatgagtatg
     2881 cggcttggaa gttccaagaa tctcctatcc cagagcagca gtttggtggt aagggtgtgg
     2941 agtatgctga gtttgtcatg gaggtggtca agccttttat caagcacaaa acaggttggt
     3001 ttgatggcat gatgacgaca ggttgctcta tgggagccta tcatgcactc aatttcttcc
     3061 tccagcatcc agatgtcttt accaaagtga ttgctctcag tggtgtttac gacgcacgtt
     3121 tctttgtcgg cgattactac aacgatgatg ctatttacca aaactcgcca gtagattata
     3181 tttggaacca aaacgatggc tggtttattg accgttaccg tcaggcagag attgtgctgt
     3241 gtacggggct tggagcctgg gaacaagatg gtttaccatc cttttacaag ctcaaagaag
     3301 cctttgacca gaaacaaatt ccagcctggt ttgctgaatg gggacatgat gtcgcccacg
     3361 actgggaatg gtggcgtaaa caaatgcttt atttcctcgg taatctctat ttataaaagg
     3421 agttacctat gaattacctt gttatttctc cctactatcc acaaaacttt caacagttta
     3481 ccatcgaact agctaataaa ggcatcacag tcttgggaat tggtcaagag tcttacgagc
     3541 aattggatga gcccttgcgc aatagcttga ccgagtattt tcgtgttgat aatcttgaga
     3601 acatagatga agtcaaacgt gcagttgctt ttctctttta taaacatggt ccaattgacc
     3661 gcatcgagtc tcacaatgaa tactggcttg agctagacgc aacactcaga gaacaattca
     3721 atgtttttgg tgccaaacca gaggatctca aaaagacgaa atataagtct gaaatgaaga
     3781 aacttttcaa aaaagcaggt gttcctgtgg tacctggagc tgttatcaag acggaagcag
     3841 atgttgatca agcagtgaaa gaaatcggtc ttccaatgat tgccaaacct gataatggag
     3901 tgggagcagc cgcaaccttt aaacttgaga cagaagacga tatcaatcac ttcaagcaag
     3961 aatgggacca ttcaaccctt tatttctttg aaaaatttgt cacttccagc gaaatctgta
     4021 cctttgacgg gctcgtggac aaggatggaa agattgtctt ctcaacaacc tttgactacg
     4081 cctatacacc gcttgacctc atgatttata agatggacaa ttcttattat gtgctcaagg
     4141 atatggatcc taaactgcgc aagtatgggg aagcaattgt caaagaattt ggtatgaaag
     4201 aacggttttt ccatattgag ttcttccgtg agggggacga ttatattacc atcgagtaca
     4261 ataaccgccc tgcaggtggt tttaccattg atgtttataa ctttgctcat tccttggacc
     4321 tttatcgtgg ctatgcagct attgtcgcag gagaggagtt cccggcgtca gactttgaaa
     4381 ctcagtattg tttggctact tctcgccgtg caaatgctca ctatgtttat tcagaagagg
     4441 atttgcttgc caaatatagc cagcagttca aggttaaaaa agtcatgcca gctgccttcg
     4501 cggaacttca aggagattac ctgtatatgc tgacaactcc gagtcgacaa gaaatggatc
     4561 agatgattgc tgatttcgga caacgtcaag aataagaact atcggattaa ggaaattcac
     4621 tcccttaatc cttttgtttt gtctggtaaa aaataagagc atcctaacaa ggtagctatc
     4681 ataaaacttg ttcgataact atttgaagca ggattaggtg gctagaaatt gaattttaac
     4741 atttcaattt agtcatagta ttgtgttttc gtatccttaa atcagctaaa aggatccatg
     4801 acgacaccta tacgatatag ttttcaggat accaaaccag tctattaata ctcaatgaaa
     4861 atcaaagagc aaactaggaa gctagccgca ggttgctcaa aacactgttt tgaggttgtg
     4921 gatagaactg acagagtcag tatcatatac ctacggcaag gtgaagctga cgtagtttga
     4981 agagattttc gaagagtata aatcttattg atgaactgct tgcagtctga gaaaaaatga
     5041 gcttggatat tatttccaaa ctcacttaaa gtcaatttca atccactaga acaagcctag
     5101 tacagttcca tcgctttcaa catccatgtt gagagctgct ggacgttttg gaagacctgg
     5161 catggtcata acatcaccag ttaaggcaac gatgaagcct gcacctaatt ttggtaccaa
     5221 ttcacgaatg gtaatttcaa agttttctgg tgctccaagc gcatttggat tgtctgagaa
     5281 actgtattga gttttagcca tacagattgg caatttgtcc caaccgtttt gaacgatttg
     5341 agcaatttgt gtttgagctt tcttctcaaa gttcactttg ctaccacgat agatttcagt
     5401 gacaattttt tcaatctttt cttggacaga aagggcatta tcgtacaaac gtttatagtt
     5461 agctggattt tcagcaattg tcttaacaac tgtttcggca agtgctactc caccttctgc
     5521 tccatcagcc cagacactag ccaattcaac tggtacatcg attgaggcac agagttcttt
     5581 taagactgca atttcagctt ctgtatcaga tacaaattcg ttaatagcta caactgctgg
     5641 aataccgaac ttacggatat tttcaacgtg gcgtttcaag ttagcaaaac ctgcacgaac
     5701 tgcctctaca ttttcttcag tcagagcgtc tttagccaca ccaccattca tcttaagggc
     5761 acgaagggtt gcgacaatga caactgcatc tggagatgtt ggcaagtttg gtgtcttgat
     5821 atcaaggaat ttctcagcac caaggtccgc accaaaacca gcttcagtaa cagtgtaatc
     5881 agccaagtga agggctgttg tcgtcgccaa aacagagtta cagccatgag cgatattggc
     5941 aaatggacca ccgtgtacaa aggcaggtgt accgtaaatt gtctgaacca agtttggctt
     6001 aatagcatcc ttcaaaatca aagccaaggc accctcaacc tgcaaatcac ctacagaaac
     6061 aggcgtacgg tcatagcgat aaccaataac gatattcgcc aaacgacgtt tcaagtcctc
     6121 gatgtccgtt gccaagcaaa gaattgccat gatttctgaa gcaactgtaa tatcaaaacc
     6181 gtcctcacgt ggaataccgt ttagaggacc accaagacca acagtcacat ggcggagcgc
     6241 acggtcattc aagtccacaa cgcgtttcca gaggatacga cgttgatcaa ttcccagctc
     6301 attcccttgg tgcaagtggt tgtcaatcaa ggcagaaagg gcattgttgg cagttgtaat
     6361 agcatgcata tctccagtaa agtggaggtt gatgtcttcc attggcagaa cttgtgcata
     6421 cccaccacca gcagcaccac ccttgatccc catgactgga ccaagagacg gttcgcggat
     6481 agcaatcatg gttttcttgc caatcttgtt caaggcatcc gcaagaccaa tggtaagcgt
     6541 cgactttcct tcacctgcag gtgttgggtt gatggcagta accaagatca atttaccgac
     6601 tggattgctc tcaactgcac gaattttatc aaagctgagt ttagccttgt actttccgta
     6661 caactccaaa tcgtcataag aaataccaag tttctctaca acatcaacaa ttggcttcaa
     6721 ctcaatactc tgtgcgattt caatatctgt tttcattcaa aattcctcta acctcttata
     6781 tgataattca ttatatcaca aaacaagatt tttaacatcc taaaactctc taaacgttcg
     6841 taaatatctc tgtttttaag gattttagag tcctttctta aattttatat ggctttatag
     6901 tttgaaacta tagtaaatct tcgtttttac caaaaattta tcactttcat tttacttacc
     6961 gcttattttt gtgtacaata gtgctatgaa aattttagtt acatcgggcg gtaccagtga
     7021 agctatcgat agcgtccgct ctatcactaa ccattctaca ggtcacttgg ggaaaattat
     7081 cacagagact ttgctttctg cagggcatga agtttgttta attacgacaa aacgagctct
     7141 gaagccagag cctcatccta acctaagtat tcgagaaatt accaatacca aggaccttct
     7201 aatagaaatg caagaacgtg ttcaggatta tcaggtcttg atccactcaa tggctgtttc
     7261 tgactacact cctgtttata tgacagggct tgaggaagtt caggctagct ccaatctaaa
     7321 agaattttta agcaagcaga atcatcaggc caagatttct tcaactgatg aggttcaggt
     7381 tttgttcctt aaaaagacac ccaaaatcat atccctagtc aaggaatgga atcccactat
     7441 tcatctgatt ggtttcaaac tgctggttga tgttaccgaa gatcatctgg ttgacattgc
     7501 acgaaaaagt cttatcaaga atcaagcaga tttaatcatc gcgaatgacc tgactcaaat
     7561 ttcagcagat cagcaccgag ctatatttgt tgagaaaaat cagcttcaaa cagtccagac
     7621 taaagaagaa attgcagaac tcctccttga aaaaattcaa gcctatcatt cttagaaagg
     7681 aaaactatgg caaacattct cttggctgta acgggttcaa tcgcctctta taagtcggca
     7741 gatttagtca gttctctaaa aaaacaaggc catcaagtca ctgtcttaat gactcaggct
     7801 gctacagagt ttatccaacc tttgacacta caggtactct cacagaatcc tgtccacttg
     7861 gatgtcatga aggaacccta tcctgatcag gtcaatcata tcgaacttgg aaaaaaagca
     7921 gatttattta tcgtggtacc tgcaactgct aacactattg caaaactagc tcacggattt
     7981 gcggacaaca tggtaaccag tacagctcta gccctaccaa gtcatattcc caaactaata
     8041 gctcctgcta tgaatacaaa aatgtatgac catccagtaa ctcagaataa tctgaaaaca
     8101 ttagaaacct acggctatca gctgattgct cctaaggaat ccctactagc ttgtggagac
     8161 cacggacgag gagctttagc tgacctcaca attattttag aaagaataaa ggaaactatc
     8221 gatgaaaaaa cgctctaata ttgcacccat tgctatcttt tttgctacca tgctcgtgat
     8281 acactttctg agctcactta tctttaacct ttttccattt ccaatcaaac cgaccattgt
     8341 tcatattcct gtcattattg ccagcattat ttatggtcca cgagttgggg ttacacttgg
     8401 atttttgatg ggattactta gcttgacggt taacacgatt acgattctac cgacaagcta
     8461 cctcttctct cccttcgtac caaacggaaa catctactca gctatcattg ccatcgtccc
     8521 acgtattttg attggtttaa ctccttactt agtctataaa ctgatgaaaa acaagactgg
     8581 tctgatttta gctggagccc tcggttcctt gacaaatact atctttgtcc ttggaggaat
     8641 cttcttccta tttggaaatg tttataatgg aaatatccaa cttcttctgg caaccgttat
     8701 ctcaacaaat tcaattgctg aattggtcat ttctgcaatt ctaaccctag ccattgttcc
     8761 acgactacaa accttgaaaa aataaaaaca atctccgctt tcaaacctga aggtggagat
     8821 tttgctctat acagtttctt tttaatgaaa tctttactat agtaaaacga aataagaaca
     8881 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgga
     8941 ctattctagt ttccatctac tataactttt aactaaaaaa tgatctataa acccaagtaa
     9001 attcttgctt tattgtctgg tttattgtac catactagtg tatatgcagt taaaaaggag
     9061 attcttgtga atacacggaa aaagacacaa tttatgacaa tgacagccct tttaacggct
     9121 attgcgattt tgattccaat tgttatgcct ttcaagattg tcattccacc tgcttcctat
     9181 actttgggga gccacatcgc tatttttata gccatgttct tgtcgccctt gatggcagtt
     9241 tttgtcatcc tagcctctag ttttggattt ttgatggctg gctatcccat ggttatcgtt
     9301 tttcgggctt tttcccatat atcttttggt gctttaggag ctctttacct acaaaaattc
     9361 cccgataccc tagataaacc aaaatcttcc tgggttttca attttgtttt ggctgttgtt
     9421 catgcccttg ctgaagtatt agcctgtgtc cttttttacg caacttcggg taccaatgta
     9481 gaaaatatgt tctatgttct atttgtacta gttggatttg gcacaattat ccatagtatg
     9541 gtagactata cattagcact agctgtctat aaagtgcttc gaaaacgccg ttaaaaggaa
     9601 aagctatgac aaaagatcgc aaacaagccc tgctccaact cttgaaagaa gctcctaaag
     9661 cccttaatgg ccaaagtttg gcggaacatt ttcatgtcac acgtcaggtc attgtgcagg
     9721 acattgcaat tttgagagcc gatggcgctc ctatcctatc caccaatcgt ggctacatct
     9781 ataagcaaat tgataccaat ccttatgttc acaaactttt caaagtgaaa catgaagttg
     9841 aagaaatcgg tcaggaactc cttgctattg tcgataatgg tgggcatgtc cagaatacct
     9901 tgattgacca tcccgtttat ggagaaattg aaaccttgct taaactgtct tgtcgcagag
     9961 atgtgcaaca ttttctagaa caagtcgagc attctgactt tagacctcta tctgaattga
    10021 cagatggcat ccattaccac ctagtcgaag ccgaaacaca acaagacctc cactatatcg
    10081 aggaggcctt ggatcagcta ggttatttag taaaagacta gaagattttc ttttcccaat
    10141 cgtcttctgt acggcgagga tagaaaaact ctgacttgtc gggagcttcc atcatttcca
    10201 tcaatggtag catatcatag gccagattta agtttggaat ctggtctttt tgcacccaag
    10261 aaacttctcc ctcttctgaa gattgaaggg taccagagaa ctcagtcgcc ttataacaaa
    10321 tgacaatata gcgcccacct gtatctagtg gccaattttt aatgccgaca agttgaggat
    10381 tttggatagt caaccctgtt tcttcgtaga tttcacgaat gacagactcc gcaaaagcct
    10441 catcattttc tacatgacct ccaggaaagg cataaccaga ccagcgattg ttttcagggg
    10501 cgcgatactg catcaccacg cgctgagttt cgaggtcttc aatcagacag atatttgtta
    10561 aaattgttaa ttgggaacgg gacataaatt tactcgcttt ctaatttatt aaattttcag
    10621 actt
//
LOCUS       DAVLWA010000061        10601 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLWA010000061 DAVLWA010000000
VERSION     DAVLWA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.061"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(117..1541)
                     /locus_tag="VYN80_001490"
     CDS             complement(117..1541)
                     /locus_tag="VYN80_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1541387.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VYN80_001491"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VYN80_001491"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1541388.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VYN80_001492"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VYN80_001492"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1541389.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VYN80_001493"
     CDS             complement(6528..7706)
                     /locus_tag="VYN80_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1541390.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VYN80_001494"
     CDS             complement(7786..8649)
                     /locus_tag="VYN80_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1541391.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VYN80_001495"
     CDS             complement(8652..10553)
                     /locus_tag="VYN80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1541392.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3187 a         2342 c         1896 g         3176 t
ORIGIN      
        1 tgtactattc tggtttcaat ctattataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctggttt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag aagattgccc
      481 tcacgtttga ttggtgtaag agcctgagct tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg taaataagcc gcacgttcaa ctgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaaacttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacgtcaagg cgaggctaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctactcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt actaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaaccac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggtag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatat gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagcc ttacctacca aatcttcctc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaat actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 agacagattt tcaattgaat ggaaatattg aagtaaaagc tgactaacct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 ggcagggaac ttgtaggcta ctgcccactt tggagcctta actgtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcacttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgcaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctcttccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat attgagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgcatggcc ttttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaatcaaaac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taagtcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa agggaagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatc tttacacctg atctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggcc ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caaaacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttggtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtagcta gcttttgctc cttcaactca acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat acttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggag
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tagctagagc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattt tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgttta
    10261 tctgtccgac gtaagtcatc aaagacatga agcatctcat catagatggt attttcagac
    10321 tcaaaacggc tatcttgagc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccactagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat catacaagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag t
//
LOCUS       DAVLWA010000062        10416 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLWA010000062 DAVLWA010000000
VERSION     DAVLWA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.062"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..389
                     /locus_tag="VYN80_001496"
                     /pseudo
     CDS             1..389
                     /locus_tag="VYN80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            475..687
                     /locus_tag="VYN80_001497"
                     /pseudo
     CDS             475..687
                     /locus_tag="VYN80_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="VYN80_001498"
     CDS             587..1033
                     /locus_tag="VYN80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1541393.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VYN80_001499"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VYN80_001499"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1541394.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="VYN80_001500"
     CDS             2639..3085
                     /locus_tag="VYN80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1541395.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="VYN80_001501"
     CDS             complement(3082..3885)
                     /locus_tag="VYN80_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1541396.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="VYN80_001502"
     CDS             complement(3894..4223)
                     /locus_tag="VYN80_001502"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1541397.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="VYN80_001503"
     CDS             complement(4168..4539)
                     /locus_tag="VYN80_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1541398.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VYN80_001504"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VYN80_001504"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1541399.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VYN80_001505"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VYN80_001505"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1541400.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="VYN80_001506"
     CDS             complement(7157..8299)
                     /locus_tag="VYN80_001506"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1541401.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VYN80_001507"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VYN80_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1541402.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3193 a         2286 c         1829 g         3108 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagc tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcataataag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatatttg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaataat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 tagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cgtaagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctc gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtggt
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagttccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa ccaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccataggcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accagggtga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga taaatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacttcatt caagcgttga agaaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca ttatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc tgccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcagg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctccccttct cctgtaagcg ttttctatgt ttttattata
    10261 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    10321 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
    10381 cgcctttctt tttctactat agtgaaatga aataaa
//
LOCUS       DAVLWA010000063        10398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLWA010000063 DAVLWA010000000
VERSION     DAVLWA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.063"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            69..737
                     /locus_tag="VYN80_001508"
     CDS             69..737
                     /locus_tag="VYN80_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1541403.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            697..1374
                     /locus_tag="VYN80_001509"
     CDS             697..1374
                     /locus_tag="VYN80_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1541404.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1375..2133
                     /locus_tag="VYN80_001510"
     CDS             1375..2133
                     /locus_tag="VYN80_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1541405.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2145..3006
                     /locus_tag="VYN80_001511"
                     /pseudo
     CDS             2145..3006
                     /locus_tag="VYN80_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3086..3733)
                     /locus_tag="VYN80_001512"
     CDS             complement(3086..3733)
                     /locus_tag="VYN80_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541406.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3730..4428)
                     /locus_tag="VYN80_001513"
     CDS             complement(3730..4428)
                     /locus_tag="VYN80_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541407.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(4438..5190)
                     /locus_tag="VYN80_001514"
     CDS             complement(4438..5190)
                     /locus_tag="VYN80_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541408.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(5190..5954)
                     /locus_tag="VYN80_001515"
     CDS             complement(5190..5954)
                     /locus_tag="VYN80_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541409.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(6050..6455)
                     /locus_tag="VYN80_001516"
                     /pseudo
     CDS             complement(6050..6455)
                     /locus_tag="VYN80_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6635..7267)
                     /locus_tag="VYN80_001517"
     CDS             complement(6635..7267)
                     /locus_tag="VYN80_001517"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1541410.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKDDQENWQEG"
     gene            complement(7301..8002)
                     /gene="pyrF"
                     /locus_tag="VYN80_001518"
     CDS             complement(7301..8002)
                     /gene="pyrF"
                     /locus_tag="VYN80_001518"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1541411.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            8323..8415
                     /locus_tag="VYN80_001519"
                     /pseudo
     CDS             8323..8415
                     /locus_tag="VYN80_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8737..9450)
                     /locus_tag="VYN80_001520"
     CDS             complement(8737..9450)
                     /locus_tag="VYN80_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541412.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(9443..10276)
                     /locus_tag="VYN80_001521"
     CDS             complement(9443..10276)
                     /locus_tag="VYN80_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541413.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
BASE COUNT         3422 a         2143 c         1718 g         3115 t
ORIGIN      
        1 aagaagattt taagtgctac aataactgta ttatttctag atgggaggtt ctatttttgg
       61 attgatccat tgttgaacaa tatctaccac tatatcaaaa ggcattcttt ctgaccttgc
      121 atattgcagt ttggggaatt ttgggatcct ttctgctcgg tttaatcgtt agtatcatcc
      181 gacattatcg aatccttgtt ttgtcgcaag tagcgacagc ctacattgaa ttgtcacgta
      241 atacgcccct tttgattcaa ctcttctttc tctacttcgg tcttccccga atcgggattg
      301 tcctatcttc agaagtctgt gcaacgcttg ggcttgtctt tttaggagac tcctatatgg
      361 cagaatcttt ccgaagtggg ctggaagcca tcagtcaaac ccagcaggag attggcctcg
      421 ctattggtct gacacctcta caggtctttt actatgtggt tcttccgcaa gcaacagcgg
      481 tggcactccc ctcctttagt gccaatgtca ttttccttat caaggaaacc tctgttttct
      541 cagcagtggc tttggccgac ctcatgtacg tcgccaagga tttgattggt ctctactatg
      601 agacagacat tgcgctagct atgttggtag ttgcttatct gatcatgctg ctacccatct
      661 cactggtctt tagctggata gaaaggaggc tccgccatgc aggattcggg aatccaagta
      721 ctctttcaag gaaataatct cctgagaatc ttacagggat tgggcgttac gattgggata
      781 tccatcctgt ctgtcctctt atccatgatg ttcggaacag tcatgggaat catcatgacc
      841 tcccattcta gaatcatacg atttttaaca cgattgtatc tggaatttat ccgtatcatg
      901 ccccagctgg tgctactctt catcgtttac tttggcttgg ctcgaaactt taatatcaat
      961 atctcaggtg agacttcagc tattatcgtt tttaccctct ggggaacagc tgaaatggga
     1021 gacttggtac gtggagctat cacttctctc cctaaacatc agtttgaaag tggacaggca
     1081 ctcggcttga ctaatgttca actttactac cacatcatca tcccacaagt cttgagaaga
     1141 ctgctaccgc aggctatcaa tcttgtcact cggatgatta aaaccacttc attagttgtt
     1201 ttgattgggg ttgtggaagt gaccaaagtt ggacaacaaa tcatcgatag caatcgcctg
     1261 accatcccaa ctgcttcatt ttggatttat ggaaccattc tagtcttata tttcgcagtt
     1321 tgctacccta tttccaaact atccactcac ttagaaaaac attggagaaa ctaaatgtct
     1381 gaaactatct tagaaatcaa ggaactaaaa aaatccttcg gagacaatcc catcctccaa
     1441 ggactttctc tagaaatcaa aaaaggggaa gttgttgtca tcctagggcc atctggttgt
     1501 gggaaaagta ccctccttcg ttgcctcaac ggcttagaaa gtattcaagg tggagatatt
     1561 tttctggatg gtcagtctat cgttgaaaat aaaaaaaatt ttcacctagt tcgccaaaag
     1621 attggcatgg tctttcaaag ttatgaactc tttccccatc tggatgtctt acaaaacctc
     1681 atcctaggcc ctatcaaaac tcaaggaagg gacaagaaag aagtaacgga agaagctttg
     1741 caattactag atcgtgtcgg tttgctggat aaacaacata gctttgcccg tcaattatct
     1801 ggtggacaga agcaacgtgt tgcaattgtc cgtgccctcc taatgcatcc agaaatcatc
     1861 ctttttgacg aggtgactgc ttcgctggat ccagaaatgg tgcgtgaggt gctggaactt
     1921 atcaatgatt tggcccaaga aggccgtacc atgattttag taacccacga aatgcagttt
     1981 gcccaagcca ttgctgaccg gattatcttc ctcgaccaag ggaaaatcgc tgaagaagga
     2041 acagctcaag ccttctttac caatccgcaa accaaacgag cccaggaatt tttaaacgtc
     2101 tttgacttta gccaatttgg ctcatatcta taaaggagat tcttatgaaa ctattcaaac
     2161 cactcttaac tgttttagca cttgcctttg cccttatctt tatcactgct tgtagctcag
     2221 gtggaaacgc tggttcatcc tctggaaaaa caactgccaa agctcgcact atcgatgaaa
     2281 tcaaaaaaag gcggtgaact gcgaatcgcc gtgtttggag ataaaaaacc gtttggctac
     2341 gttgacaatg atggttctta ccaaggctac gatattgaac tagggaacca actagctaaa
     2401 gaccttggtg tcaaggttaa atacatttca gtcgatgctg ccaaccgtgc ggaatacttg
     2461 atttcaaaca aggtagatat tactcttgct aactttacag taactgacga acgtaagaaa
     2521 caagttgatt ttgcccttcc atatatgaaa gtttctctgg gtgtcgtatc acctaagact
     2581 ggtctcatta cagacgtcaa acaacttgaa ggtaaaacct taattgtcac aaaaggaacg
     2641 actgctgaga cttattttga aaagaatcat ccagaaatca aactccaaaa atacgaccaa
     2701 tacagtgact cttaccaagc tcttcttgac ggacgtggag atgccttttc aactgacaat
     2761 acggaagttc tagcttgggc gcttgaaaat aaaggatttg aagtaggaat tacttccctc
     2821 ggtgatcccg ataccattgc ggcagcagtt caaaaaggca accaagaatt gctagacttc
     2881 atcaataaag atattgaaaa attaggcaag gaaaacttct tccacaaggc ctatgaaaaa
     2941 acacttcacc caacctacgg tgacgctgct aaagcagatg acctggttgt tgaaggtggc
     3001 cattaactct taaaagggac tggattttaa gctccaatcc ctttttaaga ttttacctat
     3061 aacatcctga gtctatctaa gatgttcaat ctgaacacag tgtacatact ttatcttcta
     3121 ttgcatatac tttatcacat aagatacgaa tatcctcttc actatgacta gcaatcaaaa
     3181 ttgttgtccc tttttcacta gagagctttc taaacaatgt tctcatattt tctacacttg
     3241 atttatccaa ggcattcata ggttcatcta gtaaaagaat agagggattc tccataattg
     3301 cttgagcaat ccctagcttt ttcctcatac ctagcgaata agttttaact ttctggtctt
     3361 tttgctcata tagaccaact attttcagtg tatcattgat ttcctgatta ccaactactc
     3421 ctcgtatgct tgccaaatat tgtaaattct taaagccact ataataattt ataaaaccag
     3481 gttcttcaat caaagctccc aaattagctg gaatttttct ctcaggaaca atattttccc
     3541 cattgattaa cacttctcca taagacggac tatataaacc agctattaat ttaaacaata
     3601 cacttttccc tgagccattc gcaccagtaa ttcctataat ttccccctgt ttacaactaa
     3661 aattaaggtt ttgaaaaaca catgtttttt ttaatttcaa ctcaatattt tttaatatga
     3721 ttatttcatt cattctataa acctcctctt ttgacgagtg aaatagaaaa tacttttaaa
     3781 aagaaagact aaaaatagca actgaagaaa taaatctcgt cctatatctc cattccctcg
     3841 attcaaaata taaaatagat aattagttcg atttcctaca aatagaccac caaacacaat
     3901 catgagtaaa aagaaactaa cgcaagcaaa gttcatttgt acattcgctc ctagtgcgta
     3961 taacataaag caaaacaaga ctaacacaca agcattcaaa gaatcaacta taaacaaaac
     4021 tcctatatcc cagtttggta actgtaacat aaattgaatc actagaactt ttaccatgat
     4081 taaaatgagt ccataaagaa aacaggtcca aatctgatac ctaatccaga gcttaataga
     4141 agaaaatcga atcacttcca gaaagaaatc ggatgctctt ctacgatata caactgctag
     4201 aaaaaatgta tgaataataa accaatgaag caaccatata gataagacta aaaaagagtt
     4261 ttgactcaga ttaggccctt ttaaaatttc tataacaagt ctatcatttc cttctaacca
     4321 ataccataat cctaaacaaa tcaatatcca aaaaagaaca taaccccaaa agtacttggt
     4381 atagattatc tgattcaacc tccacatgtg actacttaac tttcccattt ccaactttta
     4441 cactctcctc cagtttctat gaacagaaaa tacagtcaat ggaattatga tagatagtaa
     4501 tgtaagccaa taaatcaatg catacggatt ggaatttacc atatactgaa aaattggcaa
     4561 taatccaatt atcccattgt ccaactgata aatccagaat aaggattcta caaataaaaa
     4621 agtaaacaga aatactaagc tcatgtttga ttttttaaac caagaaaaac tgctaatagt
     4681 aagtaaggat aaaaagaaat agtatgctat ataagagaaa aaaaatccta taaagaaact
     4741 agcattgttt gcaatactta taccataaaa ttctcttaaa aaatcaacct cctttatctc
     4801 caaagagaag ctaaaaccat tactaaatgc aattagaaaa atcaataaaa ataaagtcgc
     4861 cgtccaaatc cccgtactaa gagctgctaa tttgaaacta aaactggtaa agtgcttaat
     4921 tgatttcaga cgaatgcgac actccaacct attaaaatag ttattcatca aataaaaaaa
     4981 gaacaatata tatgtgaacg gaaagcaata tactccagtc gtcatatctt gaagtaaaac
     5041 taagatccat tctaatacat ttggatggat tgaatattgg cgacagcgca ataaatatac
     5101 tgtactagat aaaacacagg atagcagtaa tataaaataa accaatactg ataaaaaatc
     5161 ttttttgtaa aattgaacaa attgtttcat tatacgtagt cctctgaatg tagaaaaaat
     5221 gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc actaactaag
     5281 gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc taagtaagat
     5341 agccctaaaa tagcccaaaa aataccacca accatcatat aggcaactgg gatgaaaata
     5401 gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga gaacacagcg
     5461 ccccaaactc cataccagag agtcgtcaca agactataga gcaactgatt agaatcaaat
     5521 aattctttta aggcaccact ataatctcca atataaattt cctgataagg agtcactaaa
     5581 agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga agaaataatt
     5641 gcagtactac ccacaatagc tagatacctc tttttagaaa ttcggcacaa ttgtgctgtt
     5701 agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt acagataagt
     5761 gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat
     5821 aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact catgcaaaga
     5881 agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac aagattacaa
     5941 atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca ttcttttttc
     6001 atgctacacc cacaatcaac catctttaag gcttactctg acaagtaagt taataagaat
     6061 ctggactcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt ggcgccatag
     6121 ctaatctagt accagaacca atataattgt caccacctcc attatagtac atgacaatcc
     6181 tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag gcgcgctacg
     6241 aatagaggta tttaaccttt taccgccacc agtgctgtca ctattattaa ttccagcaga
     6301 ggcgttttct ttctcaagct cactagttgt atccgaaccc caacgcggca acgttaaatc
     6361 ataatttcca atctgtctag ctaaaactgt agggagaaac aaactagatg caattgtaac
     6421 tacgcaaaaa aggaatatat ttttcttctt ttcatgacat cgtcctctta agaagtgata
     6481 aacaagatag ttgattgatc aatttgatac atattacatt ttcattatat caataaaaga
     6541 aagcgttgtc aattatttta tttaaaatat tgtcatattt taaataaaat ggtaaaagag
     6601 gctcggtagc ctcgtctctc tttatcttac tgacctaacc ttcttgccaa ttttcttggt
     6661 cgtctttaaa gcgttttaga agatcaaggc cctctggcgt gatgtaacct tcttcttggg
     6721 ctagatggat aagctcgcta tagtttgaaa gcgtcacaag tttaacacca gcatctgcaa
     6781 agttcttatc tgcttttggc aattggtagc tgaaaatcgc tacaactcca agcacatctg
     6841 ctccttctcg cttggctgct gctacagctt caagaactga accacccgtt gaaataaggt
     6901 cttcaaccac taccattttt tgaccttgag ctacgcgacc ttcgatttga ttaccagctc
     6961 catggtcttt tggtttgcta cggatgtagg caaaaggcaa gtccatctta tcagcaataa
     7021 tggctccgtg tggaatccct gctgttgcag ttcctgcaat cacttctact tcaggaaagg
     7081 cttctttgat agcttccaca aaaccatttt caattagggt acgagtttct ggataggcta
     7141 gtgtcacacg attatcagtg taaatcggtg acttgatacc agatgcccaa gtgaagggtt
     7201 cctctggttt gaggtaaacg gcttggattt tcaagaggtg gctagcgata tctttagcaa
     7261 gtgtcatggt attctccttt tatttttcta atctagttct ttaattccag tcctgtgtcc
     7321 attcatcctt gatggcatga taagctgcaa caggatcctc agcttgggta atgggacgtc
     7381 ccactacgat atagtcactg ccgatttgat aggcatcagc aggtgtcatg actcgttttt
     7441 gatctccaac tgcaacacca gctggacgaa tccctggtgt cagacagata aaatctggat
     7501 tggtagcctg cttgatgact tgtacttcct gagccgagca aacaacacca tccaagccag
     7561 cttcagctgt cttcttggca tagtgaatca cagactcttg cagactggtt tggatatttt
     7621 gaaactcctg catctgagct tctgacgttg atgtgagctg agttacagcg atcaatttgg
     7681 cttgacttcc aagaccttca cgcgcagcct tcatcatctc tacaccacca gccgcatgaa
     7741 cattggtcat atccacacca agctgagata ggatcttcat tgctgacttg actgtattag
     7801 gaatgtcatg aagtttgaga tccaaaaaga cactatgacc caaacctttt aagtaggaca
     7861 caatctcagg ccccgctgcg taataaagct ccatccctac cttgagataa aggctttctt
     7921 ctgctgggaa aagagctaaa aattccttga ccgcctcaaa actaggaaaa tcaagagcaa
     7981 tgattggacg atgttctcgc ataggtttct ccttttacct ttactagtga gagagtctgg
     8041 ccaacagaag ccacgaaaaa aggaacctgc caacagcaag gttccacgaa aaatgggtag
     8101 tctccaccct ttcaccgtct aaccttagct gcctctctgg actgctttaa aggtttttta
     8161 ctattctatt atttctacta gcacttgtca agtaaaaaca tggtcacgta tgtgttgact
     8221 gctattgaac acctcacaag actctttcca aaagaatagt taaagacttt cacttaggac
     8281 agagaaaaag agtttgcttg ttacccttta attgaacaaa tgggaattct gttttatttt
     8341 gcggatgcct attcatcttg gcaaagggga agtaatgaga atgcgaatgg acttcttcgt
     8401 gagtattttc ttaaaccgat ttggcaacaa ttaccgaaaa aatccttaat ggtgctttgc
     8461 tagcttatta atcaccgtcc ccaaaaatac ttggattaca agacaggttt tgaagcactt
     8521 atagaggaga tttaaaaatg ttgcattttt tattgcaatt tatcagttat cttatcaccc
     8581 aaaacttctg caattagtat gagcactatt gttatttttc ttttttcgta cactcaaatc
     8641 aaaatatagc aataagacta ttctgtagaa ttgctgtcaa aaagatcagt tcatttttcc
     8701 ttcccacttg tgtttataat cattaaaata actcaattaa gaaattttct ttttcctaat
     8761 agttatcgta attaaagtta atagaagaac aatccaaata agtagtgtta gattaggaat
     8821 tagagtttca ttccatgaaa atccctccca ttttgtgaaa aacttagtaa acagtcccat
     8881 aaaactataa tctgttatct tcgcaactgt cttatccaaa ccagatagca tcggtaagaa
     8941 agcaactaaa atcatagcag gaagactgat aacctgagct actacttggc tcttcgcggt
     9001 taaacctatc aataaataga ataaaatgat ggataaagag gttagaagaa cgattgtaat
     9061 ataattaaaa gtatgtacaa ttgtaactcc taaaataaga ggagtagttc ccataatcac
     9121 aaaagttagc aaaaaaggaa gaaacatcgt tgataaaatg tattcggatc ctttaacacc
     9181 actcaacaga agagtttgta aattgtactt ttctttttct tcagacaaga taatagttat
     9241 aggacttcca acagccaaag ccaaagaaaa aggtaaacac atcatcaaaa gaactaatgc
     9301 ctgttgatcg ttgaccttcc cctgtgtttc cataagatat ttataaaaat atgtgaaagc
     9361 aaaaggcact aaaacttgca ataaaatact cttattggaa aggataattt gacaacgcaa
     9421 ccataccaaa gccagaaacc gtttaagcat ttagctttcc tcctgttaat gtgataaaaa
     9481 tatcttctaa agtaggctca catgaatgaa ttgaaaaaat attttccaga tcagcctgag
     9541 atacctgttc tagtgatgta aaatcaaaag ttatctgatt cccattataa tctgtaacct
     9601 taatcttttt atctttatta tatctttgaa tgagttcaga aggagctcct tgctcaacta
     9661 atttcccttt atttaataag gcaacataat cacataaaag agttgcttca ttcatatcat
     9721 gagtcgttag aaaaatcgtt gtccctgctg ttttcaattc taaaattaac tcatgaattg
     9781 ttcgagaagt tgtgggatct agacctgagg tcggttcatc cagaaagagt acagcgggtt
     9841 tgttgataag agctcgtgct aaaagcattc gttgcctcat tccagtggat aattttcctg
     9901 ctaccatctt gcaactatca tataatccta ctcgctttaa caaattatca acacgtaatt
     9961 tactaatatt ataaaattta ctataaaaaa gaagattgtt atacagagac attttctcat
    10021 aaaatccact tgtatcgcta accaatccaa ttctctttaa ttcaccgctt gttaaatttt
    10081 gagatttttg tcccaaaata atagattgtc ctttatcggc aaggagctgc ccagtcagaa
    10141 tattaatcgt tgtggtcttt ccagaaccag atggtcctaa aaatccaaaa atctgacctt
    10201 ctttaacagt gaaacttata tcatttaaag ctattttatc accaaaactt tttgataaat
    10261 ggctaacttc aatcatactc attgcccctc ctttatattc tacattttta cttaacatct
    10321 atttacttag ctttatcaaa taaacgtctt aaaaaaaaca ataacattaa gcatataacc
    10381 gttagcaaga ttgtttca
//
LOCUS       DAVLWA010000064        10393 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLWA010000064 DAVLWA010000000
VERSION     DAVLWA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.064"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1674
                     /locus_tag="VYN80_001522"
     CDS             <1..1674
                     /locus_tag="VYN80_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1541414.1"
                     /translation="NEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSP
                     SWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEA
                     AVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFETNHPAIK
                     HFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYG
                     RRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDL
                     NKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRR
                     LKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPG
                     DLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLSK
                     VFNNQYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKMIE
                     DAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            1718..6931
                     /locus_tag="VYN80_001523"
     CDS             1718..6931
                     /locus_tag="VYN80_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1541415.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            7185..8036
                     /gene="ylqF"
                     /locus_tag="VYN80_001524"
     CDS             7185..8036
                     /gene="ylqF"
                     /locus_tag="VYN80_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1541416.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            8023..8802
                     /locus_tag="VYN80_001525"
     CDS             8023..8802
                     /locus_tag="VYN80_001525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1541417.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8818..10368
                     /locus_tag="VYN80_001526"
     CDS             8818..10368
                     /locus_tag="VYN80_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1541418.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3545 a         1717 c         2130 g         3001 t
ORIGIN      
        1 aatgagtggt ttaaagaaca aactaaagca tatattgtag aagaaaaatc tactattaaa
       61 gaggtaagtg ataagcaatc aatagctggt agtccatatt caattggagt atatgataga
      121 ttaactagcc catcttggaa atatccaagt atggtgttgc cgctattaac attacctgaa
      181 aaatcggtgt ttattatagc caacatttcg actataggtt tcggtgctta tgatagatat
      241 agaagtaaag aacatccagc aggaactgat ttaaatgact atgttgagaa aaaagctaaa
      301 gaagcggctg ttagatttag agatcactat gattattggt atagaatact agatgataaa
      361 aataaagaaa aattatatag aagtgttcta gtctatgatg catttagatt tggtacagat
      421 gaaaaagaag ataaagatac atatcaagca acttttgaaa ctaatcatcc agctataaaa
      481 catttcttcg gtccagcggg aaataatgtt gttcataatt ctaatggagc atatgctaca
      541 ggggatgcat tctattatat ggcatatcgc atgcttgata aagatggtgc ggtaacttat
      601 acacacgaaa tgacacataa ctcagataga gaaatttatc ttggaggcta tggaagacga
      661 aatggacttg gaccagagtt ttatgctaaa ggactgctac aagctcctga tcatcctaat
      721 gatccaacag tcactattaa ttccatatta aaatatgatc aatcagaaga atcaacaagg
      781 cttcaagtag cagatccaac tcaacgattt ggaagtgttg atgatctaaa taagtatatg
      841 cataatatgt ttgatgtaat atacatgctt gaaatattag aaggtaaagc tgtagctaaa
      901 ttagatacaa atcaaaaata tgatttatta agaaaaatag aaaatgaata taaacctgat
      961 cctgatggaa attctgttta tgcaacgaat gttgtacgta gattaaaacc agaagaactt
     1021 actaaattaa ctacatttaa cagtttgata gaacatgata ttataacaag aagaggttat
     1081 gtagatgaag caacttataa gcggaatggc tactatacaa ttaatctatt ctcaccaatt
     1141 tattcagcat tgagcagtaa aataggaaca ccaggagatt taatgggacg acgtattgca
     1201 tttgagttat tggcagccaa ggggtacaaa gacggtatgg ttccatatat ttctaatcag
     1261 tatgaaaaag aagcgaaagc tcaaggaaaa gttattacat catatggtaa acaaataggt
     1321 ttagttactg atgaaattgt tctttcaaaa gtatttaata accagtataa ttcttggata
     1381 gatttcaaga aagatatgta taaagaaaga gaagataaat ttggaaagtt aaataaagtt
     1441 agttttatcg atccaaacgg ttcttgggct cgacaacaga aagtgactat tgataatatt
     1501 aatagacttg aaaaaatgat tgaagatgca gtaaaatttg atgctgagga tgaagttgcc
     1561 aaattatatc ctgagacaaa tagtagagtt cttaaactga aaaaagcaat ctttaaagct
     1621 tatcttgacc aaaccggtga ttttagaagt tcaatttttg agaataaaaa atagtgttta
     1681 ctattaggaa ataaaaagtg aaggtggagg attagaattg tcattattaa aaaaagataa
     1741 attttccatt cggaaaataa agggtattgt tggttcggta ttcttgggga gtcttttatt
     1801 tgcaccgtca gttgttggtg catcgactta tcattactta gattatagta gtttgacaca
     1861 aactgaacgt gatcaactca aacaaggtag acctgatgaa tccaaagagt catatgcttt
     1921 ggattatgaa aaagacgcac taccaaacac aggaagttct caatctatta tgactgcatt
     1981 gggtttgtta gctatcggga gtcttattgt gattatcaca aaagataata gaaataaaaa
     2041 aatagctaca tttttgattg taggggcgac aggtttagtt acactatcaa ctgcttcagc
     2101 acttaatttg aatgctaaca tccatgagtc tggacgtgat ggtgtgttgc aaatttctgg
     2161 ttacagatat gttggttact tggagcttga tgataaaact gtttcgtcgg tttctccagc
     2221 atcaactgtt tcgccagttg agcaacccaa agttgtaaca gagaaaggcg aaccggaagt
     2281 tcatgagaag cctgactata cgcaaccaat aggtgcaaac cttgttgagc ctgaagttca
     2341 tgagaaacta gcatacactg aaccagtagg gacaacagga gtagatgaaa atggaaactt
     2401 gattgaacca cctgttaacg acatcccaga atatactgag ccagtaggta caacaggagt
     2461 ggatgaaaat ggaaacttga ttgagccacc tgttagcgac attccagagt atactgaacc
     2521 aatatctaca gtttcagaag ttgcatcaga aagagaagag ttgccttctt tacataccga
     2581 tattcggaca gagactattc ctaaaaccac tatagaagaa tctgatccta gcaaatttat
     2641 aggcgatgat tctgttagac aagttggtga ggatggcgaa cgtcaaattg ttactagcta
     2701 tgaagagtta catggcaaaa aaatcagtga cccagttgaa acagtaacta ttttgaaaga
     2761 aatgaagcct aaaattcttg taaaaggtac caaagaaaaa cctaaagaaa aaacggctcc
     2821 tgttttgacc ttagatagaa caaatacaaa tgtattaaac cgatccgcaa ctctttctta
     2881 tcatttagtt aatacagatg gagtcacaat caataaaatt actgcgacaa ttaaagatgg
     2941 aaatgaaatt gtcaaaacag tagatttgac ttcagagcaa ttagataagc aggtagaaga
     3001 tttaaaattc tacaaggact ataagataga gaccactatg acctatgacc gtggcaaggg
     3061 agaagaaact gcaacattag aagagaaacc acttcgcttg gatttgaaaa aagttgaaat
     3121 taaaaatatc gcatctacta atttagtgaa ggtaaatgac gatggtactg aaacacctag
     3181 tgattttatg actgaaaaac cttcagatga agatgtgaag aaaatgtact taaaaattac
     3241 tagtcgtgat aataaagtaa cacgtttagc agttgataaa attgaagaag tgactgaaga
     3301 aggtaagaaa ctttataaaa tcactgccga agctcaagat ttgattcaac atacagaccc
     3361 aacgaaggtt cgtaacaagt atgttcatta tattgaaaaa ccagttccaa aagttgatga
     3421 cgtttattat aactttaaag agttagtaga tgccatgaat gctgataaaa atggtacatt
     3481 taaaatcgga gcagatttga atgcaacgaa tgtaccaaca cctaacaaac aatatgttcc
     3541 aggtacgttt aagggacatc tatcaagtgt tgatggtaaa caatatacaa ttcataacat
     3601 tgcccgtccc ttatttgacc gtgttgaaaa tggttctgtg aaaaatatta atttaggtaa
     3661 tgtggatatc aatatgcctt gggctgacgg aatagcacct gttgccaata tggtgaaaaa
     3721 tgcaactgtt gaagatgtta aggtaactgg taatgtagtt gctaataaca atattgcggg
     3781 tatcgtcaat aaaatcgata gtggtggtca gttgacaaat gtagccttca tcggtaatct
     3841 tacaggtgtt ggtgataaag gtcaatatat ggctggcatt gccggtgaga tttggagagg
     3901 taatcttgca aaagcatatg tcgaggctga tattgttgct aacagagccc gtataggtgg
     3961 tttagttgct aaaacagata atggtaacga ctctatggga attggtaaat acggttcaat
     4021 tcgtaaatca gtcaccaaag gaaccattaa gactaaagtt ttgtttgaaa caggtggatt
     4081 tatcaatagc aatcttcctt ttggtaaact tgaagataat atttcaatga tgagagttga
     4141 aaatggtgaa gagttctttg gttcttccga tttggactac gatggtggtt atttcacaaa
     4201 tggctggtta gagcgtaact ttgttgtcaa aggtgtcagt tcaggtaagc attcatacaa
     4261 acgttctcgt gacaaaatta aagaaattag tcaagatgaa gctaataaga gaattgcaaa
     4321 ttttggactt actgctgata aatacgaaat caacgaacct gtggtcaatc gcttaaatcg
     4381 tttaaccaga agagaagatg aatataagtc aactcaagac tacaaatccg aacgtgattt
     4441 agcttatcgt aatattgaga aacttcaacc attttataat aaagaatgga ttgtcaacca
     4501 aggtaacaaa cttgcagaag attcaaactt agctaagaaa gaagtccttt ctgttacggg
     4561 gatgaaggat ggtcaattcg tgaccgattt atcagatatt gataagatta tggttcacta
     4621 tgctgatggt actaaggaag aaatggatgt tactaagaat actgattcta aagtccaaca
     4681 agtgcgtgaa tatagtgtat ctggtcttgg tgatgttgtt tatacaccga atatggttgt
     4741 taaaaatcgt gataaattga ttgctgatgt taaatctcaa ttatcaagtg tggaattgat
     4801 ttcacaagaa gtaagagatt taatgagtag acgtgataaa cctgcagaga atacggatga
     4861 acgtaagaat ggttatatta aagatctata cttagaagaa agctttgccg aagtgaagca
     4921 aaatctagat aagttagtca aatccttggt cgaaaatgaa gaccatcaat tgaatggtga
     4981 tgaagctgcg ataaaatcac ttctcaagaa agtggaaacc aataaagcta aaatcatgat
     5041 ggctctgact tatctcaatc gttactatga tatcaaatac ggtgacataa gtataaaaaa
     5101 catcatgatg ttcaaacctg atttttatgg taaaacacca agtgttattg acagactcat
     5161 caacatcggt tcaagtgaaa agaacttgaa aggggatcgg actcaggatg cttaccgtga
     5221 aatcatcgca ggtaatactg gtaaaagtaa tttgcgtaac ttcttagaat acaacatgcg
     5281 tctgtttaca gaggataaag acatcaatga ttggtttatc cactcagcta agaatgttta
     5341 tgtttcagaa cctaagacaa caaatacaga gttgaaagat aaacgtcatc gtgtgttcga
     5401 tggtttagat aacggtgttc atggtcgtat gattttacca cttctaacgc ttaaagatgc
     5461 ccatatgttc ttaatttcaa catataacac gatggcgtac agttcatttg agaaatatgg
     5521 aaaacataca gaagaagctc gtaatgagtt caaaactaag attgatgaag ttgcacatgc
     5581 tcaacaaact tatttagatt tctggtccag attagctctg cctaatgttc gtgatagact
     5641 tcttaagagt caaaatatgg taccgacacc agtttgggat aaccagacct acaatggttc
     5701 tcctgtaggt cgtcgaggat ttgacagtaa aggtaaccct atagctccaa ttcgtgaatt
     5761 atatggtcca acatggcgac accatgatag agactggcgt atgggcgcta tggcatcaat
     5821 attccctaat ccaaataatg atgacaaggt cttgtttatg gtgacggata tgattagccc
     5881 atttggtatt tcagccttta ctcacgaaac aacacacgta aacgatagaa tgctttactt
     5941 cggtggtcat aagcatcgtc aaggtacaga cgttgaagcc tatgctcaag gtatgttgca
     6001 aacacctgac agctcaacga ctaatggtga atacggcgca cttggaatta acatggctta
     6061 ccatcgtcca aatgatggta accaatggta caacccagat cctgacaaat taaagacacg
     6121 tgatgacatt gaccgctaca tgagaaacta caatgaagct atgatgttgc ttgatcatgt
     6181 agaagctgat gctgtacttc ctaaaattaa aggagacaat agtaagtggt tcaagaagat
     6241 tgacaaagaa atgcgctcta agattcagta taatgatctt ttaggtccaa accaatggga
     6301 tagtatccgt gatttgaaag atgaagaaaa ggtaatgact ttatctagcg tcaatgattt
     6361 agtggataat aacttcatga caaaacatgg aaatccaggt aatggacgtt atagaccaga
     6421 agattttaca ccaaactcag cttacgtaaa tgtcaatatg atggcaggaa tttacggtgg
     6481 gaatacgagt caaggtgctc ctggttcatt atcatttaag cacaatgctt tccgtatgtg
     6541 gggctattat ggttatgaaa atggctttat tagttatgta tcaaataaat acaaagcaga
     6601 agcagataaa aacaaccacg gtttactaag cgataagcta ataattaata aagtatcgaa
     6661 aggaaacttt aatactcttg aagagtggaa gagacattgg tacggggaag ttcttgctaa
     6721 agctaagaaa ggttttgagg ctatcgatat tgatggcgtc catatcagta actatgatga
     6781 gttaaggcct ttatttgata aagcagttga agaagatttg aagaaaccag atgacttctc
     6841 tcacactgtt gctcttaaat ctaaggtctt caaagccctt cttaaaaaca cagacggttt
     6901 ctttaataaa ctgtttaaag aagacattta aatgtttaga atatagaagg aacagttttt
     6961 tgctccctct gaaaagtcat catttgatgg cttttttcta tatagggtac ttgaaaggct
     7021 atcatcggac aaaataaaga aggcatgata taatataagg tagatttcta tgtcataaaa
     7081 caaaaaactg tttgaacatc attcatttga gaacccttta tgttcaaacg atagtaaaat
     7141 aaaataggat ctctaaaccc ttgctacgaa aggaaaaaaa ctcaatggct actattcaat
     7201 ggtttcctgg tcacatgtct aaagcgcgtc gacaggtgca ggaaaattta aaatttgttg
     7261 attttgtaac gattttggtg gatgcacgct tacctctatc tagtcaaaat cctatgttga
     7321 ccaagattgt tggtgataaa ccaaaactct tgattttaaa taaggcggat ttggctgatc
     7381 cagcaatgac caaggaatgg cgtcagtatt ttgaatcaca aggaatccaa actctggcta
     7441 tcaactccaa ggagcaagtg actgtaaaag ttgtaacaga tgcggccaag aagctcatgg
     7501 ctgataagat tgctcgccag aaagaacgtg ggattcagat tgaaaccttg cgtactatga
     7561 ttatcgggat tccaaacgct ggtaaatcaa ctctgatgaa ccgtttggct ggtaaaaaga
     7621 ttgctgttgt tggaaacaag ccaggggtca caaaaggtca acaatggctt aaaaccaata
     7681 aagacctgga aatcttggat acaccgggga ttctctggcc taagtttgag gatgaaactg
     7741 ttgcacttaa gttggcattg actggagcta tcaaggatca gttgcttcct atggatgagg
     7801 ttaccatttt tggtatcaat tatttcaaag aacattatcc agaaaagctg gctgaacgct
     7861 tcaaacaaat gaaaattgaa gaagaagcgc ctgtgattat tatggatatg acccgcgccc
     7921 tcggtttccg tgatgactat gaccgttttt acagtctctt cgtgaaggaa gtccgtgatg
     7981 gcaaactcgg taactatacc ttagatacat tggaagacct cgatggcaac gattaaagaa
     8041 atcaaagaat tccttgtgac agtcaaggag ttagaaagcc ctattttttt agagcttgaa
     8101 aaggataatc gctcaggagt tcaaaaggaa atcagcaagc gtaaaagagc cattcaagct
     8161 gaattagatg aaaatttgcg cttggaatcc atgctttctt atgaaaaaga actttataag
     8221 caaggattga ccttaattgt aggtattgat gaggttggtc gtggtcctct tgctggtcct
     8281 gtagtcgctg cggccgtcat tttacctaaa aattgtaaga ttaaaggtct caacgacagc
     8341 aagaaaattc ctaaaaagaa acatctggag attttccaag ccgttcaaga ccaagccttg
     8401 tcgattggaa ttggtatcat agataatcag gtcatcgacc aagtcaacat ctatgaagca
     8461 accaaactag ccatgcaaga agcaatctcc cagctcagcc ctcaaccaga gcaccttttg
     8521 attgatgcca tgaaactgga cttgcccatt tcacaaacct ccattatcaa aggagatgcc
     8581 aactccctct ctatcgcagc agcatctata gtagccaagg taacacgtga tgaattgatg
     8641 aaggaatacg atcagcagtt ccctggctat gatttcgcta ctaatgcagg atatggcaca
     8701 gctaaacatc tggaaggcct cacaaaacta ggagttaccc caattcaccg aaccagcttt
     8761 gaacccgtta aatcactggt tttaggtaaa aaagaaagtt aattgaaagg aaataccatg
     8821 gaggaacagt cggaaatagt ccgttctaag aaagaattcg cctttgcatc cagcactata
     8881 ctatcccaag ttggtcgagg aatcattgtc ggcctcatcg ttggaattat cgtcggatcc
     8941 tttcgtttct taattgaaaa gggcttccac ctgatacaag gagtttatca agatcaaggg
     9001 tacttagtgc gcaatctttt tgtactggtt ttgttttata tactcatctg ttggctcagt
     9061 gccaaactaa cacggtcaga aaaagatatt aaaggctcag gaattcctca agtcgaagcc
     9121 gaactgaaag gcctcatgtc cctcaactgg tggggcattc tttggaaaaa atatgtgcta
     9181 ggtattcttg ctattgccag tggactcatg ctgggtcgag agggacccag tattcaactt
     9241 ggagcagttg gtggtaaagg aattgccaag tggctcaaat ccagtccagt agaggaacgt
     9301 tccttgattg ccagtggagc tgcagcaggt ttagccgcag cctttaatgc tcctattgca
     9361 gcacttctct ttgttgtaga agaagtctat caccattttt cgcgcttttt ctgggtctca
     9421 actctagcag ccagcatcgt agcaaacttt gtgtctctac tcatgttcgg tttgacacca
     9481 gtattggata tgccagataa cattcctccc atgaccctag atcagtattg gatatatctc
     9541 gtcatgggaa ttttccttgg attttcaggt tttctctatg agaaagctgt attaaacgtt
     9601 ggaagagttt atgacttgat tggtcaaaaa atccatttgg atagggctta ttatcccatc
     9661 ttggctttta tccttatcat accagtcgga atcttcttac ctcaaatcat tggtggcgga
     9721 aatcagcttg tcctttcttt aactgaacaa aattttagtt tccaagtttt attagcttac
     9781 tttttaatcc gctttatttg gagtatgatt agctatggaa gtggactgcc aggaggaatt
     9841 ttcctcccca ttttagctct tggttctttg cttggtgcct tagttggtgt tatctgtgtc
     9901 aatcttggac ttgtcagtca agagcaattc cctatatttg tcattctagg aatgagtggc
     9961 tattttggag ccatatcaaa agctccctta accgctatga tcctcgtaac tgagatggta
    10021 ggagatattc gcaaccttat gccacttggt cttgtcactc ttgtttctta tattatcatg
    10081 gatttgctca aaggtacgcc agtctatgaa gccatgctgg aaaaaatgct tccagaagaa
    10141 gtatctagcg aaggagaagt tacacttatc gaaataccag tttctgataa aattgctggg
    10201 aaacaagttc atgaactcaa cttaccacac aacgtcctca tcacaactca agtccataat
    10261 ggcaagagcc aaacggttaa cggctcaacc agaatgtatc tgggtgatat gattcacctg
    10321 gttattccaa aaagtgaaat tggaaaagtc aaagatttgt tgttgtagta tgatcattta
    10381 cataatttat gtt
//
LOCUS       DAVLWA010000065        10388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLWA010000065 DAVLWA010000000
VERSION     DAVLWA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.065"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            9..451
                     /locus_tag="VYN80_001527"
                     /pseudo
     CDS             9..451
                     /locus_tag="VYN80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826702.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            489..779
                     /locus_tag="VYN80_001528"
     CDS             489..779
                     /locus_tag="VYN80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541419.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAK"
     gene            799..1603
                     /locus_tag="VYN80_001529"
     CDS             join(799..1167,1169..1603)
                     /locus_tag="VYN80_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1541420.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPKKELANYSGKKKCHAMKAQAIVTSQGRIVSLDIAVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYLQAQTPRKSSKLKPLTAEDKACNHV
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            1652..2116
                     /locus_tag="VYN80_001530"
     CDS             1652..2116
                     /locus_tag="VYN80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1541421.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2274..2699
                     /gene="lacA"
                     /locus_tag="VYN80_001531"
     CDS             2274..2699
                     /gene="lacA"
                     /locus_tag="VYN80_001531"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1541422.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2730..3245
                     /gene="lacB"
                     /locus_tag="VYN80_001532"
     CDS             2730..3245
                     /gene="lacB"
                     /locus_tag="VYN80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1541423.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3256..4185
                     /locus_tag="VYN80_001533"
     CDS             3256..4185
                     /locus_tag="VYN80_001533"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1541424.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            4187..5167
                     /gene="lacD"
                     /locus_tag="VYN80_001534"
     CDS             4187..5167
                     /gene="lacD"
                     /locus_tag="VYN80_001534"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1541425.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5401..6237
                     /locus_tag="VYN80_001535"
     CDS             5401..6237
                     /locus_tag="VYN80_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1541426.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6273..6590
                     /locus_tag="VYN80_001536"
     CDS             6273..6590
                     /locus_tag="VYN80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1541427.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6590..8281
                     /locus_tag="VYN80_001537"
     CDS             6590..8281
                     /locus_tag="VYN80_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1541428.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8496..9902
                     /gene="lacG"
                     /locus_tag="VYN80_001538"
     CDS             8496..9902
                     /gene="lacG"
                     /locus_tag="VYN80_001538"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1541429.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9945..10130
                     /locus_tag="VYN80_001539"
     CDS             9945..10130
                     /locus_tag="VYN80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1541430.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWDTISFDSDFMPFQQS"
BASE COUNT         3280 a         1861 c         2146 g         3101 t
ORIGIN      
        1 aaaaccttgt gttttgctta gaagcggata atcaagaata actctttgat caggtagcaa
       61 gcttgttgga ggaacgagaa attgtaactc caacttatcg tgaagctttg atcacgcgtg
      121 aaaagtcatt tccaactggt ttagatatgg aatttctagg aaaggacttg caaatgtaat
      181 gattcctcac acagatattg ttcataatct agctgagaaa gtggtggttg ttcgattaga
      241 gaaaccggtg actttccata atatgattgc gccaggtaag gaagtagaag tatccttgct
      301 cttctttatc atcaataatt caagttcaag tcaaacaaat attctggctc agttgatgga
      361 ctttttcaca ggaaatggac atcttgaaga tttatcaaaa atttccgaac cagaaaaact
      421 ttatgcttac attgctgaag caaccgctta atcttgtcta ttaaaaaata aaatcggagg
      481 aaatccaaat gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa
      541 ttaaaagtgc tctagaagaa gaactttcaa accgtggtta cgatgttcac tgtgatgcag
      601 ttatggtgaa agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg
      661 ctgcaacaga ccttggtttt gaaccaggta tcccagttat cgaagctggg ccaatcttat
      721 tccgtatccc agcaatgagc gttccagtat ttgacaatat cagacttcct gcgaaataaa
      781 atatggtata gtagttctat gaatgatgaa gcaagtaaac aactaactga tgcacgattt
      841 aagcgtcttg ttggtgttca gcgtaccact tttgaagaga tgttagctgt attaaaaaca
      901 gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta aattaagcct agaagacctt
      961 cttatggcca ctcttcaata tgtgcgagaa tatcgaactt atgaacaaat tgcggctgat
     1021 tttggtatcc acgaaagcaa cttactccgt cggagccaat gggttgaagt aactcttgtt
     1081 caaagtggtg ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
     1141 gcgacggaag taaaaatcaa tcgccctaaa aaaagaatta gcgaattatt ctggtaaaaa
     1201 gaaatgccac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttcttt
     1261 ggatatcgct gtgaactatt gtcatgatat gaagttgttc aaaatgagtc gcagaaatat
     1321 cggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatct
     1381 tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagctg aagataaagc
     1441 ctgtaaccat gtgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa
     1501 aacgtttaaa atgttttcaa caacttatcg aaatcatcgt aaacgcttcg gattacgaat
     1561 gaatttgatt gctggcatta tcaatcatga actaggattc tagttttgca ggaagtctaa
     1621 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc
     1681 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagatttg
     1741 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
     1801 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcacag atgcagtgac
     1861 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataagggatt
     1921 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
     1981 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
     2041 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
     2101 gatagaagtt ttataaaaaa agtaaaaaat aaataacaaa aacaaacaca aactgttgac
     2161 ttcttcaaac aatagtagta tattatgttt gtgaggacta attgactgtt ttttaaagac
     2221 agccaataca ctgctggaat ccagcataga aaaaataaaa aggagtatac aatatgtcta
     2281 ttgttatcgg tgcagatgct gcaggtttga gattgaaaga agttgtgaaa gacttcttag
     2341 aaaaagaaaa cttccacctt gtggatgtta cagctgaagg tcaagacttt gttgatgtga
     2401 ctctcgctgt tgctgcagaa gtaaacaaag aagaacaaaa ccttggtatc gtgattgatg
     2461 cttatggagc tggtccattt atggttgcaa ctaagatcaa aggaatggtt gctgcagaag
     2521 tatctgacga acgttcagct tatatgactc gtggccacaa caactcacgt atgatcacta
     2581 tgggggcaca acttgttggt gatgaattgg ctaaaaatat cgctaaagga tttgttaacg
     2641 gtaaatacga cggtggtcgt caccaaatta gggtcgacat gttgaacaaa atgggctaat
     2701 tagattatag taagaaaggt aagttaaaaa tgagaattgc aattggatgt gaccacatcg
     2761 taacagatga aaaaatggcg gtttcagaat ttttgaaatc aaaaggatat gaagtcattg
     2821 actttggtac ctatgaccat acacgtactc actacccaat ctttggtaaa aaagtagggg
     2881 aagcagtaac tagtggtcaa gctgatctcg gggtatgtat ctgtggtact ggtgttggta
     2941 tcaacaacgc tgtaaataaa gttccaggta ttcgttctgc cttggttcgt gatatgacaa
     3001 cagcccttta tgctaaagaa caattgaacg ccaacgttat cggttttggt ggtaagatta
     3061 ctggtgaatt gcttatgtgt gatatcatcg aagctttcat tcatgctgaa tacaaaccat
     3121 ctgaagaaaa caaaaaattg attgcgaaaa ttgaacacct tgaaagtcac aatgctcaac
     3181 aaacagacgc aaacttcttc actgaattcc ttgaaaaatg ggatcgcgga gaataccacg
     3241 actaagaggt aacctatgat tttaacagtt acaatgaacc catccattga tatttcctat
     3301 cccttggatg agttgaagat tgatactgtc aatcgtgtgg tggatgtaac caaaacggct
     3361 ggtggtaagg gactcaatgt tacccgagta ctttcagaat ttggcgattc tgttcttgct
     3421 actggtttag tgggtggcaa acttggtgag tttttggttg aacatatcga taatcaagta
     3481 aagaaagatt tcttctcaat tcagggagaa actcgtaact gtatcgctat tctccacgga
     3541 gacaaccaaa cagaagttct tgaaaaaggt cctgaagtat tggaacagga aggtcaagac
     3601 tttttggaac atttcaaaaa actcttggag tcagttgaag tagtggccat ctcaggtagt
     3661 ctgccagctg gccttccagt tgattactat gcgagcttgg tagaacttgc taatcaagct
     3721 ggcaagcctg tagtcttgga ctgctcaggt gcagcacttc aggctgttct tgaatcaccc
     3781 cataaaccaa cagtcatcaa accaaataat gaagaattgt ctcagcttct tggaagagaa
     3841 gtttctgagg atttggatga attaaaagaa gtacttcaag aacctttgtt tgcagggatt
     3901 gaatggatta tcgtttcact tggtgccaac ggtacttttg ccaaacatgg tgacactttc
     3961 tacaaggtag atattcctag aattcaggtg gtaaatcctg ttggatctgg agactctact
     4021 gtggcaggaa tttcttcagg acttcttcac aaagaatcgg atgcagaatt actcatcaag
     4081 gcaaatgtcc ttggtatgct caatgctcaa gaaaaaatga ctggtcatgt caacatggcc
     4141 aactatcaag ctctatatga tcaattaata gtaaaagagg tataaaatgg ctttaacaga
     4201 acaaaaacgt gtacgcttag aaaaactttc tgatgaaaat ggtatcatct cagctcttgc
     4261 atttgaccaa cgtggtgctt tgaaacgcct catggctcaa caccaaacag aagaaccaac
     4321 tgtggcccaa atggaagaac ttaaagtctt ggtagcagat gaattgacta aatacgcttc
     4381 atctatgctt cttgaccctg agtatggact tccagcaact aaagctcttg atgaaaaagc
     4441 tggtcttctc cttgcttatg aaaaaacagg ttatgacaca acaagcacaa aacgcttgcc
     4501 agactgcttg gatgtttggt ctgcaaaacg tattaaagaa gaaggtgcag atgcagttaa
     4561 attcttgctt tactatgatg tagatagctc agacgaactc aatcaagaaa aacaagccta
     4621 catcgaacgc atcggttctg agtgtgtggc tgaagatatc ccattcttcc ttgaaatcct
     4681 tgcttacgat gaaaaaattg cggatgcagg ttctgtagaa tacgctaaag taaaaccaca
     4741 caaagttatc ggcgctatga aagtcttttc agacccacgc tttaacattg atgtcttgaa
     4801 agttgaagtt cctgttaaca ttaaatatgt tgaaggcttc gctgaaggtg aagtagttta
     4861 tacacgtgaa gaagcagcag ccttcttcaa agcgcaagat gaagcaacga acttgccata
     4921 catctacttg agtgctggtg tatcagctaa actcttccaa gatactcttg tatttgctca
     4981 tgaatcaggt gcaaacttta acggagttct ttgtggccgt gctacatggg cagggtcagt
     5041 tgaagcttac atcaaagatg gtgaagcagc agctcgcgaa tggcttcgca caactggatt
     5101 tgaaaacatt gatgagctca ataaagttct tcaaacaaca gcgacttcat ggaaagaacg
     5161 tgtgtaagaa agtcctccta gtttaggaac atgaatctaa aaaaatttaa aaaaagttgt
     5221 atgtaaaggt ttacaaaata acttacttgt gctatactta aatcacaagt taatacaagg
     5281 tgagtgttac taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc
     5341 tagttcattt gtggtcattt tttgtactta tataccttta agatataaaa ggaggttgac
     5401 atgtatcgaa ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga
     5461 gaagaggtga ttgtaattgg taaggggatt gcattcggaa agaagaaggg ggatttgatt
     5521 gctgaaaatc aggttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt
     5581 atggctcttc tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat
     5641 aagctatcaa agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat
     5701 catatttact gttcttatca agctctaact caaggaaggt acaaggatag taatctgcca
     5761 gatatttccg ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac
     5821 cgtcagaagc tagcagatca ttttcctgag gacgaaatta ttcggattgc ttatcatttc
     5881 attaatgctg aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa
     5941 attctcagga atgttgaaga agttttaacg gactatgcaa ttcaacgaac taaaaagaat
     6001 aaccatttct atgatcgctt tatgatccat ttgaattatt tcttggatta tttagacaga
     6061 tctagagatg ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca
     6121 aaagccttcg agattggttc caagatctat gatgtgatta cgcaacatac gggtcttgat
     6181 ttgtataaaa gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa
     6241 tttatttaaa actatataag gagaattcta tcatgaatag agaagaagta acattgttag
     6301 gttttgaaat cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg
     6361 ctgctgaagc tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta
     6421 ttgcagaggc tcaccacgcg caaacaagtc tattgactaa ggaagcttct ggtgaggact
     6481 tagcgtatag tgtgaccatg atgcatggtc aagatcactt gatgacaact atcttgttaa
     6541 aggatttgat gcaccatttg atcgaactct acaagagagg agttaaataa tgaacaaact
     6601 aattgcattt atcgagaaag gaaagccttt ctttgagaaa ctatctcgta atatctatct
     6661 tcgtgctatt cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt
     6721 tatcttgatt gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc
     6781 ctttctgatg aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac
     6841 aacagctaag tcattgaccg actcagtaaa ccgtagcatg gagaaaacca atcaaatcaa
     6901 ctatatgtca acgctgttag cagcgattgt tggtttgttg atgttggcag ctgatcctat
     6961 cgaaaatggt ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc
     7021 tgcctttgtt actgtagcca tctataaggt ttgtgttaag aacaacgtca ctattcgtat
     7081 gcctgacgaa gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct
     7141 atctgttgtt tctctttatg ctcttgactt attagcacgt cattttgttg gttctagtgt
     7201 ggcagaatca atcggtaaat tcttcgcacc actcttctca gcagcagacg gataccttgg
     7261 tattaccatt atctttggtg cctttgcctt cttctggttt gttgggattc atggtccatc
     7321 tatcgttgaa ccagctatcg cagctattac ctatgccaat gccgaagtca acttgaacct
     7381 tctccaacaa ggtatgcacg ctgacaagat tcttacttct ggtacacaaa tgtttatcgt
     7441 taccatgggt ggtactggtg cgacactggt tgttccattc atgttcatgt ggttaacaaa
     7501 atcaaaacgt aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa
     7561 tgaaccaatc ttgtttggtg cacctcttgt tttgaatcca atcttcttca ttccatttat
     7621 ctttgctcca attgcaaacg tatggatttt caaattcttt attgaaacac ttggaatgaa
     7681 ttcatttact gctaatctac catggacaac cccagctcca ctaggtctag ttcttggtac
     7741 gaacttccaa gttctatcat tcattcttgc tgctcttcta atcgtggttg acgttgtcat
     7801 ttactatcca ttccttaaag tttatgacga acaaattctt gaagaagaac gttcaggtaa
     7861 gtctaatgat gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc
     7921 tattcttgaa aaagcgggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt
     7981 cctcgttctc tgtgctggtg gaggtacaag tggactcctt gcaaatgctt tgaataaggc
     8041 agcagcagaa tacaatgtcc ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga
     8101 aatgttacca gagtttaatc tagttatcct tgcccctcaa gttgcttcaa actttgaaga
     8161 tatgaaggca gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata
     8221 catcaaatta actcgtgatg gaaaaggtgc cctagcattc gtacaagagc aattcgatta
     8281 aggctaggaa ctgtgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta
     8341 atcgccttat taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattca
     8401 gtgacttggt acaagaagtg agatggagaa gcatggctca ctgactcctc tcctctcact
     8461 tttactttat ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact
     8521 ttatttttgg tggcgcaaca gctgcttatc aagcagaagg tgctacacat actgatggaa
     8581 aaggaccagt tgcttgggat aaatatcttg aggataacta ctggtacact gccgaaccag
     8641 ctagtgattt ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca
     8701 atggcattcg aatttctatt gcctggtcac gtattttccc gactggttac ggccaagtaa
     8761 atgctaaagg tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg
     8821 agccttttgt aactcttcat cactttgaca cgccagaagc tctccactca aatggagact
     8881 tcttaaaccg tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat
     8941 ttccagaagt aaactattgg acaaccttta atgaaattgg accaatcggt gatggtcaat
     9001 atttggttgg gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac
     9061 accacaatat gatggtgtct catgcatgcg cggtaaaatt gtacaaagag aaaggctata
     9121 aaggggaaat tggtgttgtt cacgccctgc ctactaaata tcctctagat cctgaaaatc
     9181 cagcagatgt tcgtgcagca gagttggaag atatcatcca caataaattc atcttagacg
     9241 caacttatct aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag
     9301 tcaatggtgg tagtttggat cttcgtgaag aagattttac agcattagaa gctgcaaaag
     9361 acttgaatga tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg
     9421 gagaaactga aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca
     9481 aaggtattgg tcgtcgtgta gctcctgact atgtatcacg cacggattgg gattggatta
     9541 tctaccctca aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat cctaactaca
     9601 agaaaattta tatcactgaa aatggtctcg gctataaaga tgagttcgtt gataacactg
     9661 tttacgatga tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga
     9721 ttgcagatgg agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat
     9781 ggtcaaacgg ttatgagaaa cgttatggtc tcttctacgt agattttgaa actcaagaac
     9841 gttatcctaa gaaatcagct cactggtaca agaaagtagc ggaaactcag attatagact
     9901 agtagaatta gtcattagat atagaatttt agtgagtcaa aaagatgttc aaagatttta
     9961 tccaatctat ttatgaaaaa gtttatatta taaatttcga aaaatgctct caaataccgt
    10021 gtttgacgag tgaagaattg aaaagtcttg gaaaatggta tgtctcgact ggtaaagaat
    10081 gggatactat ctcctttgat tcagatttta tgccgtttca acaatcgtaa ccaatttctc
    10141 aaaaaagtta aatcttatat ttagtactct gtaaaactct tatctaatca cgttgcttat
    10201 actcaatgca tagctttgag gaattgggca aaaagtcttt gatatagaaa aacgcatagt
    10261 atcaggtgtt gaaacacctg atactatgcg ttttattgtg ggaagattta ctttttttct
    10321 tctgaaattg agttgttacc caggctcttt cagtttatta aggcttgatg actttaatgt
    10381 gtttagat
//
LOCUS       DAVLWA010000066        10346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLWA010000066 DAVLWA010000000
VERSION     DAVLWA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.066"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(113..550)
                     /locus_tag="VYN80_001540"
     CDS             complement(113..550)
                     /locus_tag="VYN80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1541431.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(635..3268)
                     /gene="polA"
                     /locus_tag="VYN80_001541"
     CDS             complement(635..3268)
                     /gene="polA"
                     /locus_tag="VYN80_001541"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1541432.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEYGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAKGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCEDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPDIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3519..3869
                     /locus_tag="VYN80_001542"
     CDS             3519..3869
                     /locus_tag="VYN80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1541433.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            4084..4200
                     /locus_tag="VYN80_001543"
                     /pseudo
     CDS             4084..4200
                     /locus_tag="VYN80_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
     gene            4193..4426
                     /locus_tag="VYN80_001544"
     CDS             4193..4426
                     /locus_tag="VYN80_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541434.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            4553..4834
                     /locus_tag="VYN80_001545"
                     /pseudo
     CDS             4553..4834
                     /locus_tag="VYN80_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4968..5936)
                     /locus_tag="VYN80_001546"
     CDS             complement(4968..5936)
                     /locus_tag="VYN80_001546"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1541435.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6081..6864)
                     /locus_tag="VYN80_001547"
                     /pseudo
     CDS             complement(6081..6864)
                     /locus_tag="VYN80_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(6889..7386)
                     /locus_tag="VYN80_001548"
     CDS             complement(6889..7386)
                     /locus_tag="VYN80_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1541436.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     AVVEL"
     gene            complement(7459..8820)
                     /gene="radA"
                     /locus_tag="VYN80_001549"
     CDS             complement(7459..8820)
                     /gene="radA"
                     /locus_tag="VYN80_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1541437.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8834..9349)
                     /locus_tag="VYN80_001550"
     CDS             complement(8834..9349)
                     /locus_tag="VYN80_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1541438.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9351..9794)
                     /locus_tag="VYN80_001551"
     CDS             complement(9351..9794)
                     /locus_tag="VYN80_001551"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1541439.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            10099..10248
                     /locus_tag="VYN80_001552"
     CDS             10099..10248
                     /locus_tag="VYN80_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541440.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         3210 a         2482 c         1854 g         2800 t
ORIGIN      
        1 caacctcaaa acggtgtttt gagcagcccg cggctagttt cctagtttgc tagtgtgctc
       61 tttgattttc attgagtata acacaaaagg tagcccatca gctacctttt tcttatgctt
      121 cctcaatcaa gcgagtatgt tctctcttga tacagcgatt catcacgata tcatcacatc
      181 caccatcacg caaaatctct ttcgcttcta aactttcaag tcctagctgt gcccaaaaaa
      241 tcttggcatc agctttgaga aaatcacgcg ccacatcggg cagaaattca ctgcgacgat
      301 aaacattgac aatatctaca ggaaaaggaa tttcagcgag gctagcataa gccttttcac
      361 ccaagatttc gccacctgcc gccttgggat tgactgggat gattttatag ccccgagcct
      421 gcatttcctt tgttactcga ttgctggttg tttcttcacg gtcagacaaa cccaccacag
      481 caagggtttt actcgttgcg agatactgac gaatcacgcc atcacttgga ttgataaatt
      541 cttgactcat agaaatcctc ctttttcatc agtatagcac attttgaaaa ggtttgcaga
      601 attatactac aaaaaaggag gactagcccc ctttttattt agcctcgtac caggttgccc
      661 cttcattctc atctgcgata agaggaacac tgagttgaat ggcttcttcc atggtttgtt
      721 tcaccaattt tttcatctct accaattcag atttaggcac ttcaaggacg atttcatcgt
      781 gcacttgtaa cagcatctta gtctgataac cacctgcaac caaggcttta tccagctgaa
      841 tcatggcaat cttgagaata tctgctgccg aaccctggat aggtgagttg atagcagttc
      901 gctccgcaaa accacgaata ttgaagttgc gcgaattgat atctggcaac tcacggcgac
      961 gcttaaagag ggtctctaca tagcccttat cacgcgcctc ccgcaccact tcatccatgt
     1021 agtttttaat atctggaaaa cgttcaaagt aggtatcaat gtaggctttg gcttccttac
     1081 gactaattcc caaattatta gacaagccaa agtctgaaat cccataaacc actccaaagt
     1141 taactgcctt ggcattgcga cggtcatttg cagtcacatt atcaggacgc tcaatgccaa
     1201 agacccgcat ggctgtcgaa gtatggatat ctgccccctc ttggaaggcc ttaatcaagt
     1261 gctcatcctt agaaatatgc gccaaaacgc gcaattcaat ctgtgaatag tcagagctga
     1321 gtagcacact atcctcccac tctggcacaa aagccttccg aatcaagcgc ccttgttcca
     1381 atcgggcagg aatattttgc aagtttggat ccacactaga caaacgcccg gtctgggtca
     1441 aatcctgcat atagcgagta tgaatctttc catcagccaa aatccagtcc tgcaagccaa
     1501 ttacataagt agattgaatc ttagcaattt gacggtaatc caggattttc ttaacaatcg
     1561 gagcaatagg agcgagacgc tccaacacat ccactgctgt cgaataacct gtcttggttt
     1621 tcttagtgta ttctagagga agtcccaatt tctcaaagag aagcacgccc aactgcttag
     1681 gcgagttgac attaaactcc tcaccagcca gctcgtaaat ctcttgagtc agtttttcaa
     1741 tgacaagctc attttcagcc tgcatctcaa gcaaggtctc tttcttgacc acaatcccag
     1801 caatttccat cttggcaaga acaaaagcca gaggttgctc catatcataa agaagctcta
     1861 attgcccatt ttcgctgagt ttttcaagta aaataggctc tgtttctacc aaaacagcaa
     1921 gtttacaagc taagtgttcc aagaatttct cacgttcagg aatggccttt ttaacaccct
     1981 taccgtagaa agtttcatca tcaaccaagt aagtctgacc ataaagacta gcgatggtcg
     2041 caatttcatt gtcctccaca gtcgaaagga ggtatttagc caaacggatg tcaaaagcag
     2101 gcgcctgcaa atccacacca aaacgttgca aaagaacttt aaccttctta aagtcataaa
     2161 ctctcagaga tgttttttct aagaaatcct tgaaaatcgg gtcttgcaac agctcaagct
     2221 tgtctgtggc atagagctta tcctcacaag accaggcaaa tccaaccaaa ttatccgtat
     2281 ggtaattctc accaaaaagc tcaaagtgga agatagactc ttcactcagc atatcttgac
     2341 tgatttggtc aacaatagta aaatccaaac ccttagccac atcagctgac gacacattta
     2401 aagcctgctt gagctgtttg aatcccatct catcgtaaaa tttcccaaga ttttcaacat
     2461 ctggaccact atagaccaag tcctctaaac caatcgcaat cggtgccttg gtatcaatgg
     2521 tcgctagtgt tttagacaaa aaggcctgtt ccttgtcatt gatgagattt tccttcatct
     2581 tagaagtctt cattccatca atattttcat aaatcccctc aagcgaacca tactccagca
     2641 agagcttaat acccgtcttt tcaccgactt tggtcacccc agggatatta tccgacttat
     2701 cacccatgag cgccttgaga tcgataaact gagctggtgt gaggcccatt tcttccatga
     2761 ggtaatctgg cgtaaaggcc tcaaactcag ccacaccttt cttggaaatt tcaaccaccg
     2821 tatgctcatc cgtcagctga atcaaatcct tgtccccact gacaatggta atatcaaaac
     2881 catcctgctc tgctagctta tccagcgtcc caatgatgtc atccgcctca tactgagcca
     2941 gctcatagtg acgaatcccc atatgatcca gcaactcacg aatgaaagga aattgctcac
     3001 gaaactcatc aggagtcttg gcccgaccac ccttatagtc cgcatacatc tctgtccgga
     3061 aggtcgtctt tcccgcatca aaagccacca aaatatgact cggctcaacc cgctccaaca
     3121 aatgactcaa catcaactga aaaccataaa tcgcattggt atgcaaacca gccgcattct
     3181 taaaacggtc caactgctga tacagcgcaa aaaacgcccg aaaagctaca gaagacccat
     3241 caatcaataa taattttttc ttatccatac acccattata aaggaaagaa tcaaaaaata
     3301 ccattgggaa gagctagagc aagtattttt caaacttttt ccgaataaat agatagagcc
     3361 agagaattta gtaaacctag atttaaaaat gtgctataat atattgaatc tataatagta
     3421 caccttgact gctaaaatat ttctataaat taatttgact ttcctgatag agttattcac
     3481 atcttatttc aactcactat agaaggagga ataggaggat tctcagacat ccgggcatca
     3541 gcccaactaa tgatttgatt gctaagaaaa tattcagcaa tccagaaatc acttgtcaat
     3601 ttattcgcga tatgctggac ttgccagcaa aaaatgtgac cattttggag ggaagcgata
     3661 ttcacgtatt actctccatg ccttactcgg tgcaggattt ttataccagt atagacgtct
     3721 tggcggagtt ggataacggt actcaagtaa ttattgagat tcaagtccat catcagaatt
     3781 ttttcatcaa tcacttgtgg acttacctgt gcagtcaggt taatcaaaat cttgaaaaaa
     3841 ttcgtcagcg agaaggtgat actcactaga gctacaaaca catcgctcct gtttacgcca
     3901 ttgctatcgt ggatagtaat tatttctcag atgacctggc ttttcatagc tttagtatgc
     3961 gcgaagacac aacaggtgag gtattggcga ttaccaacaa tggacaggaa aaccatctgg
     4021 ttaagatggc attcttggaa ttaaaaaata cagagaaacc agcaaagaca aggttcgcaa
     4081 gccatggttg gagtttttcg gcaacaagcc ctttacccag caaccgcaac gagccattag
     4141 ccaagcaaat caactgctgg actacaagag ctggtccgag gaggacagga aaatgtttag
     4201 tcaactacat atgcgagaag aacaagtctt gttagcacag gactatgcct tggaaactgc
     4261 tagggctgaa ggccttgaac aaggactaga gcgtgggaaa gttgaaggaa gggcagaaag
     4321 gaaacttttt gccttcctag acatagtacg ccaaggtctt ctgacttctg aggttgccag
     4381 ccagcaatta ggtatgtcag tatctgaatt tgaggcactg ttgtaaaatg gctccataat
     4441 atccatagtg ggtaaatccc ctatggatat tatggagcct attttgtgta gaaaaaaagt
     4501 cccatatgac ctataatgaa aagcgacaaa acaactcatt agaaagaatc atatggaaca
     4561 attacatttt atcacaaaat tactagacat taaagaccct aatgtccaga ttttaaacat
     4621 catcaataag gatacacaca aggaaatcat cgccaaactg gactacgacg ccccatcttg
     4681 ccctgagtgc ggaaaccaat tgaagaaata tgactttcaa aaaccttcta aaattcctta
     4741 tcttgaaacg actggtatac ctactagaat tctccttaga aagcgtcgat tcaagtgcta
     4801 tcactgttca aaaatgatgg tcgctgaaac ttctgatgac gtatagtcat atttcttctc
     4861 tttttattat atcacagttt taaatctagc tttactagat tcaccgctac tatccattta
     4921 ttcggaaaaa agacgaaaaa acctgagaat catctcaggc ttggtcatta aatttttttc
     4981 tcaatatcga aaagtggaga aagtggtcgt ttttcatgaa tacgtacgat agcatcccct
     5041 aggagatgag cgattgaaat ctgctcaatc ttatcaatca aacgctcttc tggcagatag
     5101 atggtatcca aaacaaccaa tttcttaata gctgattttt ggatattgtc cgtagcagga
     5161 ccagaaagaa ctgggtgcgt acagcttgca tagactgcaa cagcaccagc ttccgcaaga
     5221 gcatctgccg catgacaaat cgttccagcg gtatcaatca tatcatcaat caagatacaa
     5281 gtcttgcctt caaccttacc gatgatgttc ataacttcac tagtattcat cttatcaacg
     5341 ctacgacgtt tatcaataat agcgatagat gttttcaaaa attctgccaa cttacgagca
     5401 cgagtcaccc ctccatggtc cgggctgaca accacatagt cagaaccaac cataccacgc
     5461 cgctcaaaat aatctgcaat tagaggagca cccatcaaat gatccacagg aatatcaaag
     5521 aatccttgaa tttgcgcagc atgcaagtcg atggtcaata aacgatccac tccagctact
     5581 tcaagcatat ttgcgacaag ttttgaagtg attggctcac gcgctctcgc ctttctatcc
     5641 tgacgtgcat acccatagta aggcatgaca acattgacag actctgcact cgcacgcttc
     5701 aaagcatcta ccataatcaa aatttcaagc agattgtcat ttacaggcga actagttgat
     5761 tgtaagataa agacgtgttt cccacggatt gattcttcaa tgttgacctg aatctctcca
     5821 tctgaaaatt ggcgaacact tgatttcccc aactctatcc caatctcctg cgccacacgt
     5881 tctgccaatt ctttattaga agaaagggca aacagcttta aatcagaaaa agacatgatt
     5941 tcctccggta tatatgtatc gcttgtgctt ttcacaagat tttccatcta ccattgtagc
     6001 gctttttgca ctatttttca atcaaaaata aaagaagggc accatatttg tacccttgca
     6061 tcattctttt gaaaaatatt ctaggtcatc aactcattgt gtttctcaac aaagcaataa
     6121 gcatgataat aaccatagag agcaatagcc gtaaccactg gaatcgctaa aggcaactct
     6181 gtttccaact ccacaaaagg agagttaaac aataagaccc tgtttcttgc caaccttctg
     6241 tcctttatag gctctgtaaa gcaagtaaac acctactaca gctagacctg aaaaagtcca
     6301 gtgagaggca attcctgaga tgatacgctc taaaattcgc gaaatagtaa agtcaaagcc
     6361 ctctggcaaa tccgtacgaa tataaccaat atcctcaatc atttggaatc ccaaaccgga
     6421 ggcaattcca agtaaaaaca aaaattttaa ttttcgcaca ggaatcaaag ccaaaacaaa
     6481 aacaagtggc aataatttca agggttcttc taccaaagga gccacaatag cactttcaaa
     6541 ggcatttaaa aatggactat ctgggaaaag aacccccagt aaatcatgga tataagtatt
     6601 agcaaaacta gacaaccagc ctaaaaggaa catccctccc aataaagaca gaatcaaaac
     6661 cttctttggc aattcccatt ttttcccaat acggaagaga aaataaagag ccggaatcat
     6721 gtaaaagaga gctagaaaga tagaaactcc cattagtcca tattccgcac ctgacctcga
     6781 accgtccgta tagtagatgg tttcatactg taaaccaata catagcaata aaataaaaat
     6841 aaataaaata ctgcttttct tcatacactt tctttctaaa tgaagtattt ataattctac
     6901 gactgccata cttcctgtat caacattgta aatggcacca gagataatga catcgtctgg
     6961 tattagggga gactcgataa gcagttgcat atcctcgcgt acactctctt ctatatcttg
     7021 gaagggcaag aagtcctggt ctgacacatc gacacccaat tcttccttca aatactcctg
     7081 aaaaggttca ttttcaaagg tctgagcacc acagtctgta tgatgcaata ccacaatttc
     7141 tcttgtcccc atttgttgct gggaaataac tagagaacga atcatatcct cagtcactcg
     7201 accacctgca ttccgcaaaa tatgagcatc cccaagtgcc aaacctagag cttgcgcaac
     7261 gtgcaaacgt gagtccatac aggtcacaat ggctactctg gttttaggtt taagtggcag
     7321 atttaactgc ccatgtaggg caacataagc ctgattggct tgcataaact gttcaaaata
     7381 cgacacgatt ccctccttga aaatttgata gtcaaatatt tctcctatct tatcattttt
     7441 aagagaattt gtcacggatt atgcaaagac ctttttcaag acttcctgaa tcgttgtcac
     7501 gccaatgacc tgaatttcct taggcagagt gattcctgtc aaggaattct taggtacata
     7561 aatcttagta aagcccagtt tagcagcttc gttgatgcgt tgctcaatac gattcacgcg
     7621 ccgaatctct cctgtcaagc ccagttctcc gacaaaacat tcctgaggat tagttggctt
     7681 gtctttgtag ctcgaagcaa tagcaactgc aacagccaag tcaatcgcag gttcatccaa
     7741 tttaacacca ccagcagatt tgagataggc atcctgattt tgcaagagaa gccctgcccg
     7801 tttttccaaa acagccataa tcaagctagc acggttaaaa tcaagtcctg tcgtagtacg
     7861 cttggcattt ccaaacatgg tcggtgttac caaagcctga acctccgcca aaatcggacg
     7921 cgtcccttcc atggttacaa cgatggagga accagtcgcc ccatccaaac gctcttctag
     7981 gaaaacttga ctcggattga gtacctcaac caagccgccc gactgcatct caaaaatccc
     8041 aatctcatta gtggaaccaa aacgattttt gaccgctctc aaaatacgaa aggtgtggtg
     8101 acgctcccct tcaaagtaaa gcaccgtatc caccatatgc tccaacatac gaggcccagc
     8161 caaggttcct tctttggtca catgacctac gataaagatg gcaatgttat tggtcttggc
     8221 caactgcatg agttcagcgg tcacttcacg tacctgagaa acagacccct gcacccctga
     8281 aatctcagga gacatgatgg tctggatgga atcaataatg agaaagtctg gctggatacg
     8341 ctccacttct gcacgaacac tctgcatatt ggtctctgca tagagataaa actcactatc
     8401 aatatcacct aagcgctctg cacgtagttt aatctgctgg gcagactcct ccccactgac
     8461 atagagaact gtccccactt gggacaactg ggttgagact tgtaggagaa gagttgattt
     8521 cccaatccca ggatccccac cgatgaggac gagacttcct ggtaccactc cgcctccaag
     8581 cacacggttg aattcctcca tctccgtctt ggttcgattg acattgatgg aagtcacctc
     8641 agctagtttc atgggcttgg ttttctcacc tgtcaaggac acacgcgcat tcttgacctc
     8701 ggcaacctca acctcttcca caaaagaaga ccaagaccca cagttggggc aacgtcccag
     8761 atatttaggg gaattatacc cacaattttg acatacaaat gtcgcttttt tctttgcgat
     8821 gacaaacctc tttctatatc tctaactcac actcaatcac ttggcaaaaa tcaatcttct
     8881 catttggcac aaactggcgc atgagcattc gatgagcaac aactaccaca gtctgatgtt
     8941 ctcgatactt agacatacat tctagaaacc gagacttcat ttccgtagct gtctcatatt
     9001 gaataggact attaggaagc aactccccct tgttttctaa aaacagtctt ctagctgttt
     9061 caaagttttc tattcctgtt ttatagacct gccattcatg taataaaggc tctactctta
     9121 aaggaagacc cgtagcacag accacatacg aagccgtttc taaagctctt gtgactgcag
     9181 aagatacgat tatttcagct gacgagagta aaggattttt gctcaatttc tggacttgct
     9241 gccgtcccat ctcagacaag ggtgccaaat ctatcccaaa tcctatataa gaacgctcct
     9301 ctaactcacg gtaatctggc tccccatgac gtacaaagat aatcttcatt ctagtgccct
     9361 gtcgatccaa atccaccagt tcgaacgcca tcagctgcat ctccatctgc aattaagaaa
     9421 gtagcaaaaa cagcctggac aatacgttcc ccaacttcaa gaacaacctc ttgatctgtg
     9481 atattcttca tctgcgcaaa aatatgccct tcatttccag gatttccata ataatcccca
     9541 tcaatgaccc caactgagtt aattaaaacc aagcccttct tacgaggatt tgaagaacga
     9601 tcatagaggt agagaacctc agtcggctgc atataagcct taacccctgt cggaaccaag
     9661 acaatctctc ctggcgcaac aactgtacgc acagcaacct ttaagtcgta accagccgca
     9721 tgcgctgtct cacgcttggg caataaattt tcatctgtaa aactcgaaac caattcaaaa
     9781 ccacgaattt tcataatttt ctcttttcta ttatcattta ttctagatta ttctatctta
     9841 tttattcgga aaaagcacga aaaaaagagc acacaacaat tataggcgat acgataattt
     9901 acactgtttc acaatacgtt gaaatttaga gctttaaagc gagggcaaag ttgatttttt
     9961 acatcgtttt acagaggttt acgacacttt tgcccctttt ttgccccttt gtttttcaaa
    10021 aaaacttcat caaatcgctt gactttcacg gtttaccgtg atataatata atcaagataa
    10081 ggaaaggagg tgaggaagtt gaacaaagaa gattggctta ggttactgga aaaggcgata
    10141 gataatatcc ctgaaacggt aactgctata gcaagtctgg tgactgcaat aacggtcgca
    10201 aggcaaaaca aaaagcgtaa accgaaatcc cgtcaaagaa aaaggtgaac gctaagaggt
    10261 tggggcgaaa gcccctcaca cctctatttt atcaaatgaa aagaggaaaa gcaatggtta
    10321 gtgcaatagc tatttttata attgcg
//
LOCUS       DAVLWA010000067        10311 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLWA010000067 DAVLWA010000000
VERSION     DAVLWA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.067"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(7..1221)
                     /locus_tag="VYN80_001553"
     CDS             complement(7..1221)
                     /locus_tag="VYN80_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1541441.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1671)
                     /locus_tag="VYN80_001554"
                     /pseudo
     CDS             complement(1377..1671)
                     /locus_tag="VYN80_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1833..2285
                     /locus_tag="VYN80_001555"
     CDS             1833..2285
                     /locus_tag="VYN80_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1541442.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2320..3708)
                     /locus_tag="VYN80_001556"
     CDS             complement(2320..3708)
                     /locus_tag="VYN80_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1541443.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3774..4736
                     /locus_tag="VYN80_001557"
     CDS             3774..4736
                     /locus_tag="VYN80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1541444.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4942..5952)
                     /gene="ccpA"
                     /locus_tag="VYN80_001558"
     CDS             complement(4942..5952)
                     /gene="ccpA"
                     /locus_tag="VYN80_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1541445.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6377..6976)
                     /locus_tag="VYN80_001559"
     CDS             complement(6377..6976)
                     /locus_tag="VYN80_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1541446.1"
                     /translation="MKVLVAEDQSMLRDAMCHLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(6978..8075)
                     /locus_tag="VYN80_001560"
     CDS             complement(6978..8075)
                     /locus_tag="VYN80_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1541447.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(8075..8812)
                     /locus_tag="VYN80_001561"
     CDS             complement(8075..8812)
                     /locus_tag="VYN80_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541448.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(8814..9698)
                     /locus_tag="VYN80_001562"
     CDS             complement(8814..9698)
                     /locus_tag="VYN80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541449.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(9685..9879)
                     /locus_tag="VYN80_001563"
     CDS             complement(9685..9879)
                     /locus_tag="VYN80_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541450.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(9876..10082)
                     /locus_tag="VYN80_001564"
     CDS             complement(9876..10082)
                     /locus_tag="VYN80_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541451.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         3147 a         2361 c         1861 g         2942 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaaagcaa gcctagaaag gagtcataaa gaagattaaa
     1441 catgaggaaa agcccccata tcatcaagta atcccaacgc ccacttcgtt ttgcaactag
     1501 aaataagatt agatggtaga gtacatgaaa aactaaaaat ccaataaaac ttggataaag
     1561 aagcggtacc agactctcta attgacagat catgatcact tctaccaaga ctgaaactag
     1621 gataattcca taataaagga aatttgattt ttgaaaagag tttttcatca ttctcccttc
     1681 cttcacttct ttcctgctat cctttctttt attttatcat atatttatat agagtttaag
     1741 ccctaatatt cttttgaaaa atactttttt tgctttactt ctatagtgaa aaggtttaca
     1801 attatagtaa gaaaatttca ggaggtttca ttatggcaca acgttaccaa aatatcatgg
     1861 tcgcaatcga tggttctaag gaagcggact tggcttttgt caagggagtt cattctgctc
     1921 tacgaaacga cgctaaactc accatcgcac atgtcattga cacacgcgct ctccaaagcg
     1981 tatccacctt tgatgctgaa gtttacgaag aactccaagt cgacgctgaa agtctgatga
     2041 aagagtacga aaaacgtgct aaagatgctg gggtagcaga tgttcatatc gtcattgaaa
     2101 tgggaaatcc aaagaccctg ctagcacgta ctattccaga tgccgaggaa gtggacctca
     2161 tcctcgttgg cgcaactggt ctcaacgcct ttgaacgcct cttggtcggc tcttcatctg
     2221 aatacatact ccgccatgct aaggtcgatt tgctggttgt gagagaacaa gaaaaaacct
     2281 tataatcaca aagaaaagga gcccctagct cctttttgtt tacgatttat ttctctcttt
     2341 atggcgttcg taagccttga gctggcgctg cagttccttt ttaatagcag gttctggagc
     2401 atatttttct tcccaattat ctggttttaa gattttatgg gtcactggat caaaatgagc
     2461 cttgccatct ggaaaaattt tccccatatt ggcctgatgg acaatatcaa aaatacgttc
     2521 tgggtccacc cccatcaaga caaaactgcc gtaggtgaag taaagcgtgt caatcaaggc
     2581 atccacttgc cctatcaaat cttgctgagc aggtgtcttc ttggctactt tacctgctgc
     2641 cttatcaagg gcctgatgaa gttgcgatac agcttgacca aaatcttctt cagaaggact
     2701 ggctgatcga acaaactcca ccaattcttc tattttaaaa ccagccctat gggttgcacc
     2761 ctctaaatcc caagctcgag gttcttcttg ggttcgttca tccatcatgt ggtggaaggt
     2821 cttgacctta ttgaaatgat agtcacggct gacaaaaact ttttctgaag acaaaacacc
     2881 aaaatgttcc aaggccttgt ggatcccatc ttgttgattg ctagtggtaa tgtgcttggc
     2941 agcttccttg acactgctac taccatttcc catagcgacc gacataccga cgccagccag
     3001 catttcaagg tcattatctg agtcaccaaa agccatgact tggttgaggt caaagccata
     3061 ttctttccca actcggcgaa tgccttctaa tttagaattt ccctgattga tgacatccga
     3121 tgcaaaagga ttgctacgtg tcaatttcaa gtcttcaaaa tcagctgccg ccttctcaga
     3181 ttcttctggt gtcatcagca tcaaaacttg gtaaataggt tgattcatca ggtgaagcag
     3241 gtcctcttcc ttttggggaa caaccttgct gaccatgcga ttaaaagact gactcaccgt
     3301 ccgagttaaa acagagggaa cgaagcgact aattcgttgg gaaaaagaac ccagaccaaa
     3361 ggacatgatt ttagaaccca acatggcatc cttggtccct aaggcaatct ccgtgccctc
     3421 ttttttagca tagctaatta gatggcgcaa atgtaacttg gaaatagggc tcgtgaacaa
     3481 gactctgtct ttactaaaga tatactggcc attataggtt accgcaaaat ccagatccaa
     3541 atcgtccatc aattccttaa caaaaaaagg tcctcgccct gtcgctacgc caactagtac
     3601 cccttgttct ttgacaatct taatcgcatc cttagtggat ttcaaaacac tcttgcgatt
     3661 gttgaccaag gttccatcga tatcaaaaaa aacagctttg acttccatcc tatcccaatc
     3721 tccccttttg tgatacaatg attataccac atttcagaaa gagtgagtaa atcatgccta
     3781 agaaaatcct tgttttacat acgggtggaa ctatttccat gcaggccgat gcttctggcg
     3841 ctgttgtgac gagttcagat aatcccatga accatgtgtc caacccactt gaaggaatcc
     3901 aagtccacgc cttggacttt tttaaccttc caagtcccca tatcaaaccc aaacatatgc
     3961 tggtcctcta ccagaaaatt aaagaggaag cagataacta cgatggagtg gtgatcacac
     4021 acggaaccga tactttagag gaaacagcct atttccttga taccatggaa gttccccata
     4081 tgcctatcgt tctaacagga gccatgcgta gctccaatga actcggtagt gatggtgttt
     4141 ataattacct aagtgcttta cgagtggcca gcgatgacag ggctgctgac aaaggagttt
     4201 tggtcgttat gaacgatgaa atccacgctg ccaagtatgt caccaaaaca catacgacta
     4261 atgtcagcac cttccagact ccaacacatg gcccccttgg tctcatcatg aaacaggaaa
     4321 tcctctactt caaaacagct gaacctcgtg ttcgctttga ccttgatcac atacaaggtt
     4381 tagtccctat catctcggct tatgctggta tgacagatga gctgattgat atgctggatt
     4441 tagaacactt ggacggtttg attatccaag ccttcggagc tggtaatatt cccaaagaaa
     4501 cggctcaaaa attagaaagc cttctgcaaa aaggaattcc agtcgctctg gtatcacgat
     4561 gctttaacgg tattgccgag cctgtttatg cctaccaggg tgggggcgta cagttgcaaa
     4621 aagcaggcgt tttctttgtt aaagaactca acgcccaaaa agctcgcttg aaactcctca
     4681 tcgccctcaa tgccggacta acaggacagg ctttgaaaga ctatatggaa ggctaatact
     4741 cttcgaaaat ctctgcaaac cacgtcacgt cgccttaccg tatgtatggt tactgacttc
     4801 gtcagtttca tctacaacct caaaacagtg tgttgcgcag cctttaacca gccgcctagt
     4861 ccgctctatg gtattcatta agtcaacatc tcttgtttaa gagcacaaaa tcaggaaatc
     4921 ttctcgattc cctgattttt tctatttacg ttttcgtgtt gagctacgtt ctgtcaaacc
     4981 atgaggtaag agaacttcac gttcttccaa ctcttcctta tgcataatct tggtcaacat
     5041 acgcatacta atggcaccaa ggtcataaag aggttgggca atcgttgtca agtttggacg
     5101 ggtaaagcgt gagatttgtg aatcatcact agtaataatt tcaaaatctt ctggcacaga
     5161 aacaccctta tcagccaaac cgttcaagac tcctgctgcc aactcatcac ctgtcacaac
     5221 tgctgcagtt gcatttgatg aaatcaaacg ctctgctaag gcgtaaccat catcatagct
     5281 atatttagat tcaaatacca aaccctcact ataagtgatt cctgcttttt tcaaggtttc
     5341 cttgtagcca actaaacgaa ccttaccatt gatgtcatcc actagcggac cgctaacgaa
     5401 agcaatacgc tcattttctt tagcaaggta actcactgca tcaattgttg cttgcttata
     5461 gtcaatattg acacttggca actggtgctc aacatcgaca gttcctgcga gaacaatcgg
     5521 agtacgtgaa cgcgaaaatt ctgagcgaat tttatctgtc aagtgatacc ccatatagat
     5581 aatgccatct acctgctttg aaaagagggt attgacaaca gaaacttctt tctcgttatc
     5641 ttcatcgcta ttagctagga caatattgta cttgtacatt tctgcaatat catcaatccc
     5701 cttagccaaa ctcgaaaaat aaccattggt aatatttgga atcacgacac cgacagtggt
     5761 tgtcttttta cttgcaagac cacgcgcaac tgcatttgga cgataatcca aacgatcaat
     5821 tacctctagc acttttttac gggtattctc ttttacattt ttattgccat tgaccacacg
     5881 gctgaccgtc gccatggaaa cacctgcttc acgagcgaca tcataaatgg ttactgtatc
     5941 atctgcattc attccttttc ctgtcctttc tatctcacac attcttttac aagtagaggt
     6001 actgattgaa gctctatatc tacttacaaa agtgaagatg tgaaaatttc gttttcatat
     6061 ttctacttat tccattctat cactaattgt aaacactttc aagtgttttt tgaagattga
     6121 ttgaaaaaat ttcatagaaa acctaggttt agctccttgc taccacctta gactaaacaa
     6181 aaaggaggaa actaagccct cctaaagtta tagtaaaatg aaataagaac aggataaatc
     6241 gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag
     6301 tttcaatcta ctataggtat tgttccattc actaccgtca attttagcac atagtcttca
     6361 tgaaaatatt atatcatcat aaccaaccag attcttttgc gatattagct gcctctgttc
     6421 gattaccagc atccagtttt gaaagaatat tggtgacata gtttcgaata gttccgtttg
     6481 agaggtaaag ctgatctgcg atttcttggt tggataagcc ccgggcgatt ccctttaaga
     6541 cagcaatctc ttgttctgtc aacggattgg gacgggtcat caccatttcc atcaattcag
     6601 gcgaatactc cttgcgtcct tcgaggacgg tgtgcaaggt ttgcatgagg tctgcaatgc
     6661 ttcgttcctt taatacataa gcatccactc cagccttgac cgcacgttca aaatacccag
     6721 cacgcttgaa ggtcgtcacc acaaccacct ttgtttcaag cttttctgat cgtatccact
     6781 ccaagacttc aagacctgtc ttaacaggca tttctacgtc aaggatggcg atatctacag
     6841 actccttttc taatagttgg attgcttctt gcccattctt ggcttgaagg acagactcca
     6901 catctggttg aagcgtgagc aagtgacaca tggcatctcg caacatactt tgatcttctg
     6961 cgactaatac tttcatctta gtttctctcc ttataaggta gtcgaacttg cacttccgtt
     7021 ggatgtttct gactgattac acttacttct cctgaaaatg gaaaaacacg atctcggact
     7081 gtatggagct catctccttt tagaaaagca aagccgcagc catcatcact tactgttaaa
     7141 atgagttcct tctctgtccg ttctaatttt aagtaagctt tagacgcttt ggcatgtttg
     7201 atgatattgg tcaccaactc aagcaaaatc atagaagcca ttgactccaa ttcttgagta
     7261 aggctagcag tatctagttg gttatccgtt tccacctcaa ttccagcaat ttctaacatc
     7321 tttttcacag tctctagttc ggatgtcaaa gttctagact taagattttc cacgatagtt
     7381 cgaacttcat tcatggaatc cttgctgatc tggtgaattt cttttaattc cttttccacc
     7441 tgtggataag cctccatctg aaataactgc aaggctagat cagttttaac actaagcata
     7501 gcaaaggtat gccccagact atcatgcaaa tcttgaccga tacgattgcg ttcattttca
     7561 gcaagcaata gatttatctg ggcattttgc ttagcctgtg cttctttcaa atcctccaca
     7621 attcgaatcc gaaccaagcc aaaagtcatt aaatcgacaa aagcaagaat tacaagtaga
     7681 tagaatagaa actcaacttc gattctctga aaaatcaaca gttgacccac aacaaggact
     7741 tgagcaagaa gaaaagtcca gacatgtaaa gactttaaac cacctacgct gaaatgatag
     7801 cttaagagat tggataggaa aaagaaaaac cagatataat taacagccac aaaggcagta
     7861 ttcccaacta cataagtcag caggatgccc caatatagcc aagatagacg ctggctctta
     7921 gttgttaaaa cacccaaata cgccactaca aatagaatat caatcaataa atgccaggca
     7981 gaaagccacc cagtcactac agttaggatg gggaaaacca taaaaattaa actgatccaa
     8041 aacatataat gtatgctttt cagtctttca agcattaagc attctcctta tgaccttgaa
     8101 ggtaaatggt caaaccaaac aaaactactg aaaaaacaag taaataaact gtggctgata
     8161 gattgatgcc accctcattt aagaaggtct tgagcaactc catcaactga taggtcggga
     8221 gacacttacc tactacttgc atccagtctg gaaataaaga gataggcatc cagagtccac
     8281 ctaaaacagc caaccctaga taaagaagat tgcccacgac agacatcaac tgactagttg
     8341 gtaagagagt caaggtcaaa ccaagcgcta cgaaggcaat acttcctact atcagcaaaa
     8401 gtgcagcccc aatccaattt ccaagagaca tgtccacacc tcttacaaaa tgcccaactg
     8461 agaaaaccac caagattgaa accaaataat caaccagcat acttgttatc tttgatagat
     8521 aatattctac catatttaca gggctatgac gcaatgtttt ctgccagttg ttgatcttgt
     8581 cggtatgtaa aacaactggg aatgagaaga tagctgttga catcatggaa aatgcagtca
     8641 tggagataag ataatcacgc ataaaattcg cgagttcacc tggtgtgtcc tgatagatac
     8701 cagaaaaaaa taaatagaaa gccgtcggca tccctaccga caatagataa tagattaatt
     8761 gtcgtttggt caataaaaat tctatcttac taagtgctat ccatcgtttc atcttagtta
     8821 tctcccttct gcgtctcttc aaagattgta ttcaacaaac tacgattatt aacttcgatt
     8881 tcttgcatcc tacatcctgc ttgggctaat agttcccaga aagcatctgc ttctcgagtg
     8941 actacttgta aagaatcttg ctttagggtc cagttttcaa ccaaatttga ctgctctacg
     9001 acttccttgt aagctatagg aaggataaag tgcttttcta tttcctcgct acgcatagct
     9061 agaggcgtcg tatctcgaat caactctccc ttatttaaga gcaaaatgcg gtctgcagtg
     9121 tgttccactt cctcaatata atgggacgaa tagagaatgg tgactccttg cgcttttaac
     9181 tcctggacaa tttcccaaaa acgttgacga gttgaggtat ccatggacgc agtaggctca
     9241 tctaaaaaaa caatctttgg tcgcccaatt aaggtcaaga tgaaagaaaa gagacgcttt
     9301 tgaccgcctg ataatttttc tgccaactgt tctttttgtt gcttgacaaa ctgcaatagt
     9361 tgatcaattt cctgattgct caaaggattt ggatagatac gttgaaagaa agcaatcaac
     9421 tctttaactt ttaatttctg aacgattata ttttcttgag ggaggtaagc cctagtatag
     9481 tctaacttag aactcgtcac tggtaaacct tgaatagata cttgaccgct tgtgaccagt
     9541 ttatctccaa gcaaacaatc taagagtgtt gtctttccgg caccatttgg accaattaag
     9601 gcaacacatt caccatcgtt gatttcaaag gagatgttcc gcaaaatttc cttgtctttt
     9661 attttcttac tcaatttttc aactttaacc acagtcatga gactctcctt tcaaccactc
     9721 cattcccata gggaaaacga cgaaaatcat aaatccaaac cccaaagcac cacaaatgaa
     9781 ttggcgaagc aaggcttgat caaaccaatc agtaaacatt tccactaacc ataccaagag
     9841 tgacaggccg ataaagaaat agatgatccc tctcttcatt cctcaaactc ctttttcaca
     9901 tctctgacta atttcaaacc ttctctaaca agccaagaca tgattccaaa gccagcaaag
     9961 accttccaag gaaaatggta gaaatcctca tccaatcccg aaaacatgag ataggtcata
    10021 actcctgcta ctactaaact cattgcaata atcattttat ttctcatctc ttcttccccc
    10081 atttcatact acaattatag tcttttaaaa tcagaggagg cagaagcttc tgtcactaga
    10141 aaatatgaca aatgtcataa aaattctgtt caaaacaagc aagatacact atacaataaa
    10201 acacaatcag aaaaatctaa ggcaacttcc tcaaaagaga tatcaaaccc aattcacacc
    10261 ataatgtaaa ctaatactta tttaaaatca aaaagagtag aaatttttat c
//
LOCUS       DAVLWA010000068        10197 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLWA010000068 DAVLWA010000000
VERSION     DAVLWA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.068"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(100..1767)
                     /locus_tag="VYN80_001565"
     CDS             complement(100..1767)
                     /locus_tag="VYN80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1541452.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMSAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1770..2135)
                     /locus_tag="VYN80_001566"
     CDS             complement(1770..2135)
                     /locus_tag="VYN80_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1541453.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2292..2480)
                     /locus_tag="VYN80_001567"
     CDS             complement(2292..2480)
                     /locus_tag="VYN80_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1541454.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2596..3081)
                     /locus_tag="VYN80_001568"
     CDS             complement(2596..3081)
                     /locus_tag="VYN80_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1541455.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENITQDL
                     I"
     gene            complement(3523..5067)
                     /locus_tag="VYN80_001569"
     CDS             complement(3523..5067)
                     /locus_tag="VYN80_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1541456.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5280..5582
                     /gene="gatC"
                     /locus_tag="VYN80_001570"
     CDS             5280..5582
                     /gene="gatC"
                     /locus_tag="VYN80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1541457.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5582..7048
                     /gene="gatA"
                     /locus_tag="VYN80_001571"
     CDS             5582..7048
                     /gene="gatA"
                     /locus_tag="VYN80_001571"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1541458.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            7048..8490
                     /gene="gatB"
                     /locus_tag="VYN80_001572"
     CDS             7048..8490
                     /gene="gatB"
                     /locus_tag="VYN80_001572"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1541459.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8619..9179
                     /gene="efp"
                     /locus_tag="VYN80_001573"
     CDS             8619..9179
                     /gene="efp"
                     /locus_tag="VYN80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1541460.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            9201..9590
                     /locus_tag="VYN80_001574"
     CDS             9201..9590
                     /locus_tag="VYN80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1541461.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9583..10005
                     /gene="nusB"
                     /locus_tag="VYN80_001575"
     CDS             9583..10005
                     /gene="nusB"
                     /locus_tag="VYN80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1541462.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(10054..10197)
                     /locus_tag="VYN80_001576"
                     /pseudo
     CDS             complement(10054..10197)
                     /locus_tag="VYN80_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT         2956 a         2333 c         1960 g         2948 t
ORIGIN      
        1 aacgtttatt tatgaatgtt atttttgttt ttattaaaaa gataccgatt tcatttacta
       61 aaaagaaaaa agaactgatt tctcagtcct tcattaatct tattccacac taaataggta
      121 tgggtaaaca ggttgttgac cttggtgaat ctcgacttca acgtcttcga attcttctac
      181 gatttcttga gcgatttcat tggcaagttc ttcgcttccg tcttcaccga catagaaggt
      241 tacgatttca ctgtcttcat ccaacatatg tttcaaggtt tcagtcaatg tttggtgcat
      301 atcagggttt gacacaagaa tttttccatc caccatacct aaattatcgt tttcatggat
      361 ttctaagcca tcgatcgttg tatcacgcac ggctgttgtg acgcttccgc taacgacatc
      421 gctaagagca gctgtcatac gctcttggtt ttcttcaatg gacttgcttg gatcaaaggc
      481 aagaagactt gtcatacctt gaggaagagt gcgagcctct accactaccg ctggttgctc
      541 caaaacttct gccgcagatt gagctgccat gaagatgttc ttgttgtttg gcaagaagat
      601 gatgttacgg gcattaacct gttcaacagc cttgataaag tcttctgttg aagggttcat
      661 ggtttgaccg ccttcgataa cataatccac gccttgagaa cagaagatat ctgctagacc
      721 tttaccagcc accacagcaa tcaaagcata ctctttttct tcagccgact tgataacttg
      781 agtagcttct ttctcaacct gtgcttcgtg ttggttacgc atattgtcaa cttttacctt
      841 gaccaagcta ccatatttga gaccttcttg cataacaagt cctggatctt ctgtatgaac
      901 atggactttg acaatttcat catcgttaac aacgaggaga gaatctccaa gctcatccaa
      961 gtagttacgg aattcatcgt agtcaaaatc tttagcatag gttggacctt gcttaagagc
     1021 taccatgatt tcagtacagt aaccaaacgt gatgtcctca gtcgctacgt gaccagctac
     1081 agacttatga tgctctacat tgatcatctc actcatgttg gcaggagtcg ctacaaagtc
     1141 ctcagatgca atatattcgc cagtaagggc tgaaaggaaa ccttcgtaga tgaagaccaa
     1201 tccttgacca cctgagtcca caacgccaac ttctttcaat actggaagca tgtctggtgt
     1261 tttagctaga gctgttttag caccttccaa ggctgcgctc atgacttcaa cagtgtcatc
     1321 tgtttgctca gcttttttct tagcaccgat agcagctcca cgagaaactg tcaaaatcgt
     1381 tccttcaaca ggtttcatca ctgccttata ggcaacttcc acacctgatt ggaaggccag
     1441 agccaagtct tgacctgtta actcgtcttt atccttgata gcttgggaaa atccacggaa
     1501 aagctgagac gtaatcactc ctgagttccc acgcgcaccc atcaaaagcc ctttggcaag
     1561 aatgctcgct acttctccaa ctgtagaagc tggcttgtct gcaacttctt tagcaccatt
     1621 ttcaatggtc attcccatat ttgtcccagt atctccatct ggaactggaa agacgtttaa
     1681 tgaattgaca tattcagctt gcttattcaa gcgagttgat gcagcctgca ccatttcttg
     1741 aaataagcta gtagtaattt ttgacacggt tattctccta caactttgat attttgaatg
     1801 tagacattta cagtctgagc agtaattcca agctggtttt ccaagctaaa ggcaacacgc
     1861 tcttgaatgt tttttgacac ttcactaatc tttgttccgt agcttaacac agtatataca
     1921 tcaactgcaa tactgccatc ttcggctgcc tttacgacga cacctttaga ataattttct
     1981 ttacctagca gggcttggaa attatctttg agggcatttt tactagccat accgaccaca
     2041 ccaaaaatct cagttgctgc tccacctacg acggttgcaa tcacttcatc tgttagttcg
     2101 atttgaccat cttttgtatt aatttttaca gtcatccttt ttacctcaac tagttgatac
     2161 tctattttat catatttcag cccaagtgta aaagcagaat aatgtatcag cggatattta
     2221 ctctattttt caaacgatgt tatacccaca ataaaagaaa aaagacccta aggtctcctt
     2281 tgcttttatt attaaacgcg ttcaacttta cctgatttca aagcacgagc tgaagcccaa
     2341 acttttttag gtttaccatc gataagaaca gtaacttttt gaaggtttgg ttttacggca
     2401 cgttttgttt ggttcatcgc gtgtgaacgg ttgtttcctg atacagtctt acgacctgta
     2461 aagtaacata ctttagccat tgtgttttcc tcctattaga tctaatatag cggatgtgct
     2521 agcaccacat accgtactat gttatcacac tttcttgttt tttgcaaggg aattggaaga
     2581 ttttttattt gtgtcttaaa tcaggtcttg cgtgatattt tctgctctcc acatgccatt
     2641 gttgattaac agaacaccag aattaaaatt atgtgtataa aaatcatctc taactgcagc
     2701 taagggtata gccgtcaagt ccaaatccca cagctcatct atcgattttc ttacaacaat
     2761 atctgaatcc aaatacagta cacgagactc gcttacatac tttggaataa aatacctaaa
     2821 aaaagccgca tatgaaagtc cctcaaaggg gagacgataa cctttcagaa tactactgtc
     2881 aatctgaaca ttcacaatct cactattcaa agtctctagt cttttttcca tcaattggaa
     2941 ccattctcgc ggaaggtcat cattaaaaac ataaaactta agattataat gatgaacaca
     3001 aagagatttt attgttgttt caactttatc catataagca ttatctgcac ctaagacaat
     3061 cgcttttttc tcttctttca ctctttatct tatttctttt tattcccatc atattattcc
     3121 catcatatgt ttctacgtaa ccattatttt cgcctattcg ttcgtaaaac cataccagtg
     3181 gagattttag atgaagtccc attacggttt acaattttta cattacgaca cggagtttta
     3241 caaatcgatt tcatttgcca aacgtagtta gtaaggcagt tagctaggct acctactttc
     3301 tgctacgacg gaaaaatcca caaatcaata ccattcgtgg atttttccta gtgaagcgtt
     3361 taggtaagcc gccatagcgg ttaccgatta ttatattacg acacggagtt tggcaaatcg
     3421 attctatttg ccaaacgtag ttagtgaggc agttagctag ttcgccaaat agcgactagc
     3481 gtccaacaat ttggaacttt agttccaatt gttggtactg agtcacatct tctcctctaa
     3541 ctctacgtct ggatacttgt ccgcaaacca gcggagggca aagtcatttt caaagagaaa
     3601 gactggttgg tcaaaacggt ctttggctaa gatattgcga cttgacgaca tccgttcatc
     3661 caagtcctca ggcttgatcc aacgaacggt ctttttaccc attgggttca taactacttc
     3721 tgcattgtac tcgccttcca tgcggtgttt aaagacttca aactggagtt gacctacagc
     3781 gcctagcatg tactcacctg tttggtaatt cttataaagc tgaacggctc cttcttgcac
     3841 caattgctca atccccttgt ggaaggattt ttgcttcata acattcttag cagaaacttt
     3901 catgaaaatt tcaggagtaa aggttggcag tggttcaaat tcaaacttgt ttttaccaac
     3961 tgtcaaggta tccccaactt gataagtacc ggtatcgtaa accccgataa tatcacctgc
     4021 cacggcattg atcacattct cacgactttc cgccataaac tgagtaacat tagacagttt
     4081 agccccctta ccagtacgag ggagattgac actcatgccg cgctcaaatt cgccagatac
     4141 gatacggaca aaggcaatac ggtcacggtg acgagggtcc atgttggctt ggattttaaa
     4201 gacaaatcct gagaaatcct tgtcataagg atccacaatt tcaccgtctg ttttcttgtg
     4261 accatgtggt tctggagcaa acttgaggaa ggtttcaagg aaggtctgca caccaaagtt
     4321 tgtcagggct gaaccgaaaa agacaggcgt caattctcca gccagaatag cttcctctga
     4381 aaactcattc ccggcttcat ttaaaagctc aatgtcatcc ttgacttgct cgtagaaagg
     4441 attgctacca aagagtttgt ccccgtcttc tagactggca aaacgctcat cccctttgta
     4501 aagctctaaa cgttggttat aaaggtcata caagccctca aaggctttcc ccatcccgat
     4561 aggccagttc atagggtagc tagcaatgcc caagatttct tccaattctt gcaagagatc
     4621 caaaggctca cgaccgtcac ggtccagctt gttcataaag gtaaagactg gaatgccacg
     4681 atgtttcaca acctcaaaca atttcttggt ttgagcctcg atccccttgg cagagtccac
     4741 gaccatgacc gcagcatcca ccgccatcaa ggtacgatag gtatcttctg agaagtcctc
     4801 gtgccctggc gtgtctaaga tattcacgcg cttgccgtcg tagtcaaatt gcataacaga
     4861 tgaagtaaca gaaatcccac gttgcttctc gatatccatc cagtcagatt tagcaaaagt
     4921 ccctgttttc ttccctttta ccgtaccagc ctcacgaatc tcacccccaa agtagagtaa
     4981 ctgctcagtg atggttgttt tccccgcgtc cgggtgggag ataatggcaa aggtacgacg
     5041 tttcttaatt tcttcttgaa tattcataag ttctctttct ttgattctct atttttcttg
     5101 tttcaatagc tgagaatgat ttttacattg gattttacca ttcctttcaa cactccatta
     5161 tatcggattt tagcattttt ttcaatttct atttcttttc acttccccct cccttattta
     5221 taggaaaata tggtaaaata gaacagacta aaaatcatca tttcacgaaa ggatgcaaga
     5281 tgaaaattac gcaagaagag gtaacacacg ttgccaatct ttcaaaatta agattctctg
     5341 aaaaagaaac tgctgccttt gcgaccacct tgtctaagat tgttgacatg gttgaattgc
     5401 tgggcgaagt tgacacaact ggtgtcgcac ctactacgac tatggctgac cgcaagactg
     5461 tactccgccc tgatgtggcc gaagaaggaa cagaccgtga tcgcttgttt aaaaacgtac
     5521 ctgaaaaaga caactactat atcaaggtgc cagctatcct agacgatgga ggagatgcct
     5581 aatgactttt aacaataaaa ctattgaaga gttgcacaat ctccttgtct ctaaggaaat
     5641 ttctgcaaca gaattgaccc aagcaacact tgaaaatatc aagtctcgtg aggaagccct
     5701 caattcattt gtcaccatcg ctgaggagca agctcttgtt caagctaaag ccattgatga
     5761 agctggaatt gatgctgaca atgtcctttc aggaattcca cttgctgtta aggataacat
     5821 ctctacagac ggtattctca caactgctgc ctcaaaaatg ctctacaact atgagccaat
     5881 ctttgatgcg acagctgttg ccaatgcaaa aaccaagggc atgattgtcg ttggaaagac
     5941 caacatggac gaatttgcta tgggtggttc aggtgaaact tcacactacg gagcaactaa
     6001 aaacgcttgg gaccacagca aggttcctgg tgggtcatca agtggttctg ccgcagctgt
     6061 agcctcagga caagttcgct tgtcacttgg ttctgatact ggtggttcca tccgccaacc
     6121 tgctgccttc aacggaatcg ttggtctcaa accaacctac ggaacagttt cacgtttcgg
     6181 tctcattgcc tttggtagct cattagacca gattggacct tttgctccta ctgttaagga
     6241 aaatgccctc ttgctcaacg ctattgccag cgaagatgct aaagactcta cttctgctcc
     6301 tgtccgcatc gccgacttta cttcaaaaat cggccaagac atcaagggta tgaaaatcgc
     6361 tttgcctaag gaatacctcg gtgaaggaat taacccagag gttaaggaaa ccattctaaa
     6421 tgccgctaaa cactttgaaa aattgggtgc tattgtcgaa gaagtcagcc ttcctcactc
     6481 taaatacgga gttgccgtat actacatcat cgcttcatca gaagcttcat caaacttgca
     6541 acgctttgac ggtatccgtt acggctatcg cgcagaagat gcaaccaacc ttgatgaaat
     6601 ctatgtaaac agccgaagcc aaggttttgg tgaagaagtg aagcgccgta tcatgctggg
     6661 tactttcagt ctttcatcag gttactacga tgcctactat aagaaggctg gacaggtccg
     6721 tacacttatc attcaagatt tcgaaaaagt cttcgcggat tacgatttga ttttgggtcc
     6781 aactgctcca agtgttgcct atgacttgga ttctctcaac catgacccag ttgccatgta
     6841 cttagccgac ctattgacca tacctgtaaa cttggcagga ctgcctggaa tttcgattcc
     6901 tgctggattc tctcaaggtc tacctgtcgg actccaattg attggtccca agtactctga
     6961 ggaaaccatt taccaagctg ctgctgcttt tgaagcaaca acagactacc acaaacaaca
     7021 acccgtgatt tttggaggtg acaactaatg aactttgaaa cagtcatcgg acttgaagtc
     7081 cacgtagagc tcaacaccaa ttcaaaaatc ttctcaccta cttctgccca ctttggaaat
     7141 gaccaaaatg ccaacactaa cgtgattgac tggtctttcc caggagttct accagttctc
     7201 aataaagggg ttgttgatgc cggtatcaag gctgctcttg ccctcaacat ggacatccac
     7261 aaaaagatgc actttgaccg caagaactac ttctatcctg ataaccccaa agcctaccaa
     7321 atttctcaat ttgatgaacc aatcggatat aatggctgga ttgaagtcaa actagaagac
     7381 ggtacgacca agaaaatcgg tatcgaacgt gcccacctag aggaagacgc tggtaaaaac
     7441 acccatggta cagatggcta ctcttatgtt gacctcaacc gccaaggggt tcccttgatt
     7501 gagattgtat ctgaggcaga tatgcgttcg ccagaggagg cctatgctta tctgacagcc
     7561 ctcaaggaag ttatccagta cgctggcatt tctgacgtta agatggaaga aggttctatg
     7621 cgtgtggatg ccaacatctc ccttcgccct tatggtcaag aaaaattcgg taccaagact
     7681 gaattgaaga acctcaactc cttctcaaac gttcgtaaag gtcttgaata cgaagtccaa
     7741 cgccaggctg aaattcttcg ctcaggtggt caaatccgcc aagaaacacg ccgttacgat
     7801 gaagcgaata aagcaaccat tctcatgcgt gtcaaggaag gggctgcaga ctaccgctac
     7861 ttcccagaac cagacctacc cctctttgaa atttctgacg agtggattga ggaaatgcgg
     7921 actgagttgc cagagtttcc aaaagaacgt cgtgcgcgtt atgtatctga ccttggttta
     7981 tcagactacg atgctagtca gttgactgct aataaagtca cttctgactt ctttgaaaaa
     8041 gctgttgccc taggtggtga tgccaaacaa gtctctaact ggctccaagg ggaagtcgct
     8101 cagttcttga atgctgaagg taaaacactg gaacaaatcg aattgacacc agaaaacttg
     8161 gttgaaatga ttaccatcat cgaagacggt actatttcat ctaagattgc caagaaagtc
     8221 tttgtccatc tagctaaaaa tggcggtggc gcgcgtgaat acgtggaaaa agcaggtatg
     8281 gttcaaattt cagatccagc tatcttgatc ccaatcatcc accaagtctt tgccgataac
     8341 gaagctgctg ttgccgactt caagtcaggc aaacgtaacg ccgacaaagc ctttacagga
     8401 ttccttatga aggcaaccaa aggccaagcc aacccacaag ttgcccttaa actacttgca
     8461 caggaattgg cgaagttgaa agaaaactag acagaacaaa accagcccta aggttggttt
     8521 tttcttctct accaactccc aataactatt ttggctttat ttccagagta ttttatggta
     8581 aaatgaagag taataatatt tattaaagag gtaaaaacat gattgaagca agtaaattaa
     8641 aagctggtat gacctttgaa acagctgacg gcaaattgat tcgcgttttg gaagctagtc
     8701 accacaaacc aggtaaagga aacacgatca tgcgtatgaa attgcgtgat gtccgtactg
     8761 gttctacatt tgacacaagc taccgtccag aggaaaaatt tgaacaagct attatcgaga
     8821 ctgtcccagc tcaatacttg tacaaaatgg atgacacagc atacttcatg aatacagaaa
     8881 cttacgacca gtacgaaatc cctgtagtca atgttgaaaa cgaattgctt tacatccttg
     8941 aaaactctga tgtgaaaatc caattctacg gaactgaagt gatcggtgtc accgttccta
     9001 ctactgttga gttgacagtt gctgaaactc aaccatctat caaaggtgct actgttacag
     9061 gttctggtaa accagcaacg atggaaactg gacttgtcgt aaacgttcca gacttcatcg
     9121 aagcaggaca aaaactcgtt atcaacactg cagaaggaac ttacgtttct cgtgcctaat
     9181 ctctagaaag aggtcattct atgggaattg aagaacaact tggcgaaatc gttatcgccc
     9241 cacgtgtact tgaaaaaatc attgctatcg ctactgcaaa ggtagagggt gttcactctt
     9301 tttcaaacag atcagtgtct gatacccttt caaaactttc actcggccgt ggcgtttatc
     9361 ttaaaaacgt ggacgaagaa ctcacagcag atatctatct ctaccttgag tacggagtaa
     9421 aagttcctaa ggtagcggtt gctatccaga aagctgtcaa agatgccgtc cgtaatatgg
     9481 ctgatgtaga actcgctgct atcaatattc acgttgcagg tatcgtccca gataaaacac
     9541 caaaaccaga attgaaagat ctatttgacg aggacttcct caatgactag tccactatta
     9601 gaatctagac gccaactccg taaatgcgct tttcaagctc tcatgagcct tgagttcggt
     9661 acggatgtcg aaactgcttg tcgtttcgcc tatactcatg atcgtgaaga tacggatgta
     9721 caacttccag cctttttgat agacctcgtt tctggtgttc aagctaaaaa ggaagaacta
     9781 gataagcaaa tcactcagca tctaaaagca ggttggacca ttgaacgctt aacgctcgtg
     9841 gagagaaacc tccttcgctt gggagtcttt gaaatcactt catttgacac tcctcagctg
     9901 gttgctgtta atgaagctat cgagcttgca aaggacttct ccgatcaaaa atctgcccgt
     9961 tttatcaatg gactgctcag ccagtttgta acagaagaac aataaaaaga aaaagtgttt
    10021 gatagatatc aaacactttt tctttgcctc ccactatcta aaaaaatgat aatagatata
    10081 attgtaaaca aaaatccaga taggttttgc atgattgaga aagttaaaaa aaactatggc
    10141 agagaatcgt taatctcaga ttgtcggtaa aacgataaac aagggcaaaa aagaaac
//
LOCUS       DAVLWA010000069        10130 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLWA010000069 DAVLWA010000000
VERSION     DAVLWA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.069"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            152..843
                     /locus_tag="VYN80_001577"
                     /pseudo
     CDS             152..843
                     /locus_tag="VYN80_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            897..1286
                     /locus_tag="VYN80_001578"
     CDS             897..1286
                     /locus_tag="VYN80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1541463.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(1947..2975)
                     /gene="queA"
                     /locus_tag="VYN80_001579"
     CDS             complement(1947..2975)
                     /gene="queA"
                     /locus_tag="VYN80_001579"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1541464.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            3126..3833
                     /locus_tag="VYN80_001580"
     CDS             3126..3833
                     /locus_tag="VYN80_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1541465.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            3973..4149
                     /locus_tag="VYN80_001581"
     CDS             3973..4149
                     /locus_tag="VYN80_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1541466.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            4279..5214
                     /locus_tag="VYN80_001582"
     CDS             4279..5214
                     /locus_tag="VYN80_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1541467.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            5207..5995
                     /locus_tag="VYN80_001583"
     CDS             5207..5995
                     /locus_tag="VYN80_001583"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1541468.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGVLVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            5996..6379
                     /locus_tag="VYN80_001584"
     CDS             5996..6379
                     /locus_tag="VYN80_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1541469.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            6395..6784
                     /locus_tag="VYN80_001585"
     CDS             6395..6784
                     /locus_tag="VYN80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1541470.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            6868..7998
                     /locus_tag="VYN80_001586"
     CDS             6868..7998
                     /locus_tag="VYN80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1541471.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            8003..8740
                     /locus_tag="VYN80_001587"
     CDS             8003..8740
                     /locus_tag="VYN80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1541472.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSEMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            8751..9524
                     /locus_tag="VYN80_001588"
     CDS             8751..9524
                     /locus_tag="VYN80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1541473.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            9514..>10130
                     /locus_tag="VYN80_001589"
     CDS             9514..>10130
                     /locus_tag="VYN80_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1541474.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
BASE COUNT         3025 a         1825 c         2312 g         2968 t
ORIGIN      
        1 agtatattgt ttaacgtttg atagaaaaga tgctatacta gaagtaggat gtaatggcaa
       61 aatctaatac taataagcgc tatgcaatac gtaaactcgg aaagattttt ggttctgcgg
      121 taatcgggct aactttattt ttaggaggaa tctgaataca agttttgttc atgctgctga
      181 tgggattcaa tatgtcagag atgatactag agataaagaa gagggaatag agtatgatga
      241 cgctgacaat ggggatatta ttgtaaaagt agcgactaaa cctaaggtag taaccaagaa
      301 aatttcaagt acgcgaattc gttatgaaaa agatgaaaca aaagaccgta gtgaaaatcc
      361 tgttacaatt gatggagagg atggctatgt aactacgaca aggacctacg atgttaatcc
      421 agagactggt tatgttaccg aacaggttac tgttgataga aaagaagcca cggatacagt
      481 tatcaaagtt ccagctaaaa gcaaggttga agaagttctt gttccatttg ctactaaata
      541 tgaagcagac aatgaccttt ctgcaggaca ggagcaagag attactctag gaaagaatgg
      601 gaaaacagtt acaacgataa cttataatgt agatggaaag agtggacaag taactgagag
      661 tactttaagt caaaaaaaga ctctcaaaca agagttgtta aaaaaagaac caagccccaa
      721 gttcttgtcc aagaaattcc aatcgaaaca gaatatctcg atggcccaac tcttgataaa
      781 agtcaagaag tagaagaagt aggagaaatt ggtaaattac tcttactaca atctatactg
      841 tagatgaacg tgatggaaca attgaagaaa ctacttctcg tcaaattact aaagagatgg
      901 taaaaagacg tataaggaga gggacgagag aacctgaaaa agttgttgtt cctgagcaat
      961 catctattcc ttcgtatcct gtatctgtta catctaacca aggaacagat gtagcagtag
     1021 aaccagctaa agcagttgct ccaacaacag actggaaaca agaaaatggt atgtggtatt
     1081 tttataatac tgatggttcc atggcaacag gttgggtaca agttaatggt tcatggtact
     1141 acctcaacag caacggttct atgaaagtca atcaatggtt ccaagttggt ggtaaatggt
     1201 attatgtaaa tgcatcgggt gagttagcgg tcaatacaag tatagatggc tatagagtca
     1261 atgataatgg tgaatgggtg cgttaacaga ttcctccctg cgacaaaatt gataatagta
     1321 tgtctgaata tataattata tataaaaagc tatagataag gtcatcttat agcgactata
     1381 aaaaagtgaa caagcctagt cttctgtaca ttagaattca gatttgttca ctttttgttt
     1441 aatactcaat gaaaatcaaa gagcaaacta gaaaactagt cgcaggttgc tcaaagtact
     1501 gctttgaggt tgtagataga actgacgaag tcagtcacat atataatcca aggtgacgtt
     1561 gacgcggttt gaagagattt ttgaagagta taagtctttg attttgagac taaggagagt
     1621 cagggtcaaa gtagtgaatg attctgaaat aactcataat agtctcttga gaggacagat
     1681 aagagtaaca aatatggtgc tactcatgtg tcttgcattt tgatttacaa acagcgaaga
     1741 aaatatagga agctgaaata aaatatagat gaatcaattt ggagagtcaa cgcaatctat
     1801 ggcaatattt tcgaagctag ggggaagcat tttaatttca atgtactagg cgtcttctac
     1861 agctagctag agtttcaatt gtagatgttc tttgaaataa tatcttagcg atttattggt
     1921 attagaagaa ttagagaaat tctttctcaa taaataaaca tggcgtcacc aaaactgaag
     1981 aagcggtagt gttcttggat ggcatggtgg taggcatcta agactaattc acggcctgca
     2041 aaggcagaaa ccaacatgac cagagttgat tttggcagat ggaagttggt tgagaaggca
     2101 tccacgacct tccactcata cccaggtttg ataaagatat tggtccaacc agaatctgct
     2161 tggatttgcc catcaaactt ggaaccaata gtttccaagg tgcggataga agtggttccg
     2221 acagcgatga cgcgaccacc attttctttg acagagcgaa gggtggcagc agcttcctca
     2281 gaaagttgat agaactctga gtgcatttcg tgttcgtcca gattatccac agaaacaggt
     2341 ctaaaggttc cgagtccgac atggagagtc agatagacta gatgaacacc cttagcttgg
     2401 atttctgcca gcagttcttt ggtgaagtga agaccagcag tcggtgctgc agcagagccg
     2461 ctttccttgg cgtagacggt ttgataacgt tcacggtcat ctaatttttc gtggatataa
     2521 ggtggcagag gcatttctcc cagactttcc aagacttcta ggaaaattcc ttggtattca
     2581 aagcggacaa tgcgtccccc gtgggtcaat tcttctgtaa cgacagcgct gaggcggcca
     2641 tcaccaaagc tgatacgagt accgaccttg aggcgtttgg caggtttagc cagaacttcc
     2701 cactcgtctc cactagtgtt cttaaggagg agaagttcca catgacctcc tgtctccact
     2761 ttttgaccat agaggcgggc agggagaact cgggtgtcgt tcatgacaag ggcatcacca
     2821 ggttccagca tatcaataat agagtggaaa tgtttatctt gcatttctcc tgtctcacgg
     2881 ttgacgatga ggagtttgga ggcatcacgt ttttcaaggg gcgtttgggc aatcaattcc
     2941 tctggcaagt ggaaatcaaa atcagctgta ttcatatatt tgttcattct ttctaagtta
     3001 taatcctatc cattataaca cgtttcaagt ttggacgctc tttttttaga aattaaatcg
     3061 ttttcacttg acaaaaattg gtctatacca tataataaat atagacagaa atggaggatg
     3121 aaaagatgaa agttattaaa gttgaaaacc aagttcaagg tggaaaagtt gcttttgaga
     3181 ttttgaagga aaaattggct aatggagctc aaactctagg acttgcgaca ggaagtagcc
     3241 cacttgaatt ttacaaggaa attgttgaga gtgaccttga tttttcaaat ctaaccagtg
     3301 tcaaccttga tgagtatgtg ggccttgatg gggacaatcc acagtcttat cgttacttca
     3361 tgcaagaaaa cttgttcaac caaaaaccat ttaaagaaag tttcttgcct cgtggtgtta
     3421 aggacaatgc tgaagctgaa gttgaacgct acaaccaaat tttggctgac catccagttg
     3481 acctccaaat cttgggaatc ggtcgcaatg ggcatatcgg ctttaatgag cctggtactc
     3541 catttgacag tcaaacgcat ctagtagaac ttgaccagtc tactatcgaa gccaatgcac
     3601 gcttctttgc caagattgaa gacgtcccaa cccaagctat ttcaatgggg attaaaaaca
     3661 ttttggatgc caagtctatt attctttttg cttacggtga gtcgaaagca gaagccattg
     3721 ccggaacagt atctggccca gtgactgaga acctacctgc aagtagcctt caaaatcacc
     3781 ctgatgtaac catcatcgca gatgctgaag cgcttagctt actcgaaaaa taaaaaagca
     3841 atgttctatt gaacgctttc aactcttgta taaatggaag aaaaaatcaa aattaaaccg
     3901 catttttgct tgacaattat tccttttacg tgtagaatga aatagatatt gaacttgaag
     3961 ggagtgaaaa aaatgtctaa aacagtagta cgtaagaatg aatctcttga cgacgcactt
     4021 cgccgtttca aacgtgcggt tactaaagct ggtactcttc aagaaacacg caaacgtgaa
     4081 ttctatgaaa aaccttctgt aaaacgtaaa cgtaaatcag aagtagctcg taaacgtaaa
     4141 aaattctaat tgaaatgaaa ggctagagaa atctagtcct ttttctttta aataaatact
     4201 ccaaagcctg caaaaatctg aaacttcctc ctacaatttg atataataga gagaagaact
     4261 catttgaagg aggaaatgat gtcggtttta gtaaaagaag tgattgaaaa gcttagacta
     4321 gatattgtct atggtgaacc agagttgctt gaaaaggaaa tcaatacagc ggatattacg
     4381 cgacctggtc ttgaaatgac aggctatttt gactactata caccagagcg gattcaactt
     4441 ttggggatga aggagtggtc ttatctgatc agcatgcctt ccaacagccg ttatgaagtt
     4501 ttgaaaaaaa tgtttctacc tgagacacca gcagtcattg ttgcccgtgg tttggtggtt
     4561 ccagaggaga tgttaaaggc tgctagagaa tgtaagattg ctattttaac cagccgtgca
     4621 gctaccagtc gtttatctgg agagttatct agctatctgg attctcgttt ggcagaacgt
     4681 accagtgtgc acggtgtctt gatggatatt tatgggatgg gcgtcttgat tcagggagat
     4741 agtggaattg gtaagagcga gacaggtctt gagcttgtca aacgtggtca ccgtttggta
     4801 gccgatgacc gtgtcgatat ctttgccaag gatgagatta ctctctgggg tgaaccagct
     4861 gaaattttga aacacttgat tgaaattcgt ggggttggta ttatcgatgt tatgagtctc
     4921 tacggtgcga gtgctgtcaa ggattcttca caggttcagc ttgctgtcta tttggaaaat
     4981 tacgatacgc ataagacctt tgatcgtctt ggaaacaatg cagaggaact tgaagtttct
     5041 ggcgtagcca ttcctcgtat tcgtattcca gttaaaacag gtcgtaatat ctctgttgtg
     5101 attgaggcag ctgccatgaa ttatcgtgcc aaggaaatgg gctttgatgc gacccgtttg
     5161 ttcgacgaac gactgacaag tctaatcgct cgaaatgagg tgcaaaatgc ttgatccaat
     5221 tgctattcaa ctaggacccc tagccattcg ttggtatgcc ttatgtattg tgacaggctt
     5281 gattcttgcg gtttatttga ctatgaaaga agcacctaga aagaagatca taccagacga
     5341 tattttagat tttatcttag tagcctttcc cttggctatt ttaggagctc gtctctacta
     5401 tgttattttc cgatttgatt actatagtca gaatttagga gagatttttg ccatttggaa
     5461 tggtggtttg gccatttacg gtggtttgat aactggggtt cttgtgctct atatctttgc
     5521 tgaccgtaaa ctcatcaata cttgggattt tctagatatt gcggcgccta gcgttatgat
     5581 tgctcaaagt ttggggcgtt ggggtaattt ctttaaccaa gaagcttatg gtgcaacagt
     5641 ggataatctg gattatctac ctggctttat ccgtgaccag atgtatattg aggggagcta
     5701 ccgtcaaccg actttccttt atgagtctct atggaatctg cttggctttg ccttgattct
     5761 gatttttaga cggaaatgga agagtctcag acgaggtcat atcacggcct tttacttgat
     5821 ttggtatggt ttcggtcgta tggttatcga aggtatgcga acagatagtc tcatgttctt
     5881 cggccttcga gtgtcccaat ggctgtcagt tgtccttatc ggtctcggta taatgatcgt
     5941 tatttatcaa aatcgaaaga aggcccctta ctatattaca gaggaggaaa actaaatgtt
     6001 agaagttgca tatattcttg ttgccctagc tttgattgtc tttttggtct atctgatcat
     6061 tactgtacaa aagcttggtc gtgtcatcga tgaaacagaa aagacgatta aaaccttgac
     6121 ttcagatgtg gatgtgacct tgcatcacac caatgagttg ttggctaagg tcaatgtctt
     6181 ggcagatgat atcaatgtca aggtggctac aattgatcca ctcttcagtg ctgttgcaga
     6241 tttatctcta tctgtttcag acctcaatga ccatgcgcgt gtcttgagca agaaagcttc
     6301 atcagctggt tcaaaaacac tcaagactgg tgcaagtctg tcagctcttc gtcttgcaag
     6361 taaatttttc aaaaaataaa aaaggagaat ccttatgggt aaattatcct caatcctttt
     6421 aggaacggtt tcaggtgcag ctcttgcctt gtttttaaca agtgataagg gcaaacaagt
     6481 ttgcagtcag gctcaagatt ttctagatga tttgagagaa gatccggagt atgccaagga
     6541 gcaagtctgt gaaaaactga cagaagttaa ggagcaggct acagattttg ttctgaaaac
     6601 aaaagaacag gttgagtcag gtgaaatcac tgtggacagt atacttgctc aagctaaatc
     6661 ctatgctttt caagcgacag aagcatcaaa aaatcaatta aataatctca aggagcaatg
     6721 gcaagaaaaa gccgaagctc ttgatgactc agaagagatt gtgattgata taacagaaga
     6781 ataaaccatc accatctcca gacggactat gtatctgggg atggtgattt ttatctggaa
     6841 tctagtcttt gtggtataat aattactatg cagaaaaaac caacgtcagc ctatgtgcac
     6901 atcccatttt gtacccagat ttgttattat tgtgattttt caaaggtctt catcaaaaat
     6961 cagccagtcg acagctattt agagcatctg ctggaagagt ttcgttctta tgatattgaa
     7021 aagttgtcaa ccctttatat cggtggtgga acaccgacag ccctgtcggc tccgcaactg
     7081 gaggtgttac tgaatggctt gactaaaaac ttggatttgt ctgtcttgga agagttgacc
     7141 attgaagcca atccaggcga tttggatgcg gataagatag ctgttttgaa aaattcggct
     7201 gtcaatcgtg tttcgctagg tgtccagacc tttgatgata agatgttgaa aaagattggg
     7261 cgcagtcatt tggagaagga tatttatgaa aatatcgatc gcctgaaact ggctggtttt
     7321 gacaacatct ccattgattt gatttatgct ctgcctggtc agaccatgga gcaagtaaag
     7381 gaaaatgtgg ctaaagccat tggactggat attccccaca tgagtttgta tagtttgatt
     7441 ttagaaaacc atacggtctt tatgaaccgg atgcgacgtg ggaaattgcc tctgcctaag
     7501 gaggaactag aagcggagat gtttgagtac atcattgcag agctggagcg agcgggtttt
     7561 gagcattatg agatttctaa tttctccaaa cccggctttg aaagtcgtca caatctcatg
     7621 tactgggaca atgctgaata ctatggtatt ggtgcagggg catctggtta tgtcaatgga
     7681 gtacgctata aaaatcatgg tcccattcgt cattatctca gtgcggttga ggaaggcaat
     7741 gcttgtatta cagaagatca cctgagtcaa aaggagcaaa tggaagaaga aatgttcttg
     7801 ggactccgca agaaatccgg ggtttccatg gcgcgatttg aggaaaaatt tggacagtct
     7861 tttgctggac tttatggaga aattgtcaga gatttggttc aacaaggcct catgcagatt
     7921 gagggtgacc acgttcgcat gacaaagaga ggtctctttt tgggagatac tgtagcagaa
     7981 cgatttattt tggagtagga tcatgggctt aacttatcaa atgaaaatga aaattccttt
     8041 tgatatggct gatatgaacg gtcatatcaa acttccagat gtgattttgc tgtcactgca
     8101 agtttcagag atgcaatcga ttgaactggg agttagtgat aaggccattt tggaagacta
     8161 taatctggtc tggattatcg cagaatatga tattgaggtg gttcgtttgc ctcgttttgc
     8221 ggaagaaatt accatcgaaa cggaagcctt gagctacaat cgactttttt gctaccgtcg
     8281 ctttactatt tataatgaag cggggcagga gctcatccat atgatggcga cctttgttct
     8341 catggaccgc gacagtcgaa aagtccatgc tgttgaacct gagattgtgg ctccttacca
     8401 gtctgatttt gataaaaaac ttatccgtgg accgaagtat gaatccttgg aagagccgat
     8461 cagcaaggat taccatgttc gtttttacga cttggatatg aatggtcatg tcaataacag
     8521 taaatacttg gactggattt ttgaggtcat gggagcggat tttttgaccc actatattcc
     8581 caagaaaatc aacctcaagt atgtcaagga agttcgacca ggtggggtga ttacatcggc
     8641 tgttgaacgg actggactgg aaagcaagca tgaaattaca agtgatgggg ttatcaatgc
     8701 ccaagcaatc attacttggc aagaaataaa aaaggtttag ggagaaatag atgaaatata
     8761 aaggctattt aattgattta gacggaacca tttataaggg gaaagaccga atccctgctg
     8821 gagagacttt tgttcatgaa ttgcaaaagc gggacattcc ctatctcttt gtaaccaaca
     8881 atacaacccg cactccagag agtgttaagg agatgttggc tcagaatttt aatatcgata
     8941 cgcctctatc gactgtctac acagcgactt tggcaaccat cgactatatg aatgatttgg
     9001 ggcttgaaaa gactgtctat gtcgtcggag aagcaggact caaggaagcc atcaaggcgg
     9061 ctggttatgt ggaagacaag gaaaaccctg cctacgtggt agttggtctg gattggcaag
     9121 tcgactatga aaaatttgcc acagcaactc ttgctattca aaagggtgcc cattttatcg
     9181 gaaccaatcc tgacctcaac atcccgacgg aacgtggtct tttgccaggt gctggctcac
     9241 tgattacgct gcttgaagta gcgacacgag tgaagccggt ttatatcgga aaaccaaatg
     9301 ccatcattat ggacaaggcg gttgagcact taggtttgga acgtgaagag ttaatcatgg
     9361 ttggggacaa ttatttgact gatatccggg ctgggattga caatggcatt ccaacgctct
     9421 tggtgacgac aggttttact aaggcagaag aagtagctgg cctgccaatc gcaccgacac
     9481 atgtggtttc tagccttgcg gagtgggatt ttgatgaaaa ctaaattgac cttttggggc
     9541 tctatgctct ttctcctctc cctctcaatc cttctgacca tttatctggc ttggattttc
     9601 tatcctatgg agattcagtg gctaaactta acgaatcgag tctatctaaa accagaaacc
     9661 attcaataca attttcatat cttgatgaat tatctgacca atccttttag tcaggtctta
     9721 cagatgcctg attttcgttc gtcagcagct ggtctgcacc atttcgcagt ggtcaagaat
     9781 ctctttcatt tggttcagct agtagctcta gtgacactgc caagtttcta tgtctttgtc
     9841 aataggattg tgaaaaagga ctttttgtct ctttatcgaa aaagtctcct ggctctagta
     9901 gtcttacctg tgatgattgg acttggggga gttttgattg gttttgacca attctttact
     9961 cttttccatc aaattctctt tgtgggagat gatacctggc tttttgatcc agccaaggat
    10021 cctgttatta tgattttgcc agagaccttc tttcttcatg ccttcctcct cttttttgcc
    10081 ctctatgaaa acttctttgg ctatctgtat ctgaaaagtc gtaggaagtg
//
LOCUS       DAVLWA010000070         9984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLWA010000070 DAVLWA010000000
VERSION     DAVLWA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.070"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            120..5099
                     /locus_tag="VYN80_001590"
     CDS             120..5099
                     /locus_tag="VYN80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1541475.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KATARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKYLVALLKEDKSAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTASHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5189..6385
                     /locus_tag="VYN80_001591"
     CDS             5189..6385
                     /locus_tag="VYN80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1541476.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6521..7048
                     /locus_tag="VYN80_001592"
     CDS             6521..7048
                     /locus_tag="VYN80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1541477.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7125..7481
                     /gene="glnR"
                     /locus_tag="VYN80_001593"
     CDS             7125..7481
                     /gene="glnR"
                     /locus_tag="VYN80_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1541478.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7518..8864
                     /gene="glnA"
                     /locus_tag="VYN80_001594"
     CDS             7518..8864
                     /gene="glnA"
                     /locus_tag="VYN80_001594"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1541479.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8960..9079)
                     /locus_tag="VYN80_001595"
     CDS             complement(8960..9079)
                     /locus_tag="VYN80_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1541480.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9337..9474
                     /locus_tag="VYN80_001596"
     CDS             9337..9474
                     /locus_tag="VYN80_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541481.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEDLNIRLEVK
                     N"
     gene            9567..9984
                     /locus_tag="VYN80_001597"
                     /pseudo
     CDS             9567..9984
                     /locus_tag="VYN80_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3236 a         1803 c         2163 g         2782 t
ORIGIN      
        1 ttgtctaaaa atgttttagg ataaaaatgt gattatcatg gttctagagt ttgataaaaa
       61 accacagaat ataaagcgct ttcttgaaaa caacaaaatc aatcttttag gaggagaaaa
      121 tgaagaatcc attttttgaa agacgttgtc gttacagtat tcgtaagtta tcagtaggag
      181 cctgctcgct gatgattggt gctgttttat ttgctggtcc agccttggct gaagaaactg
      241 cagttcctga aaatagcgga gctaatacag agcttgtttc aggagagagt gagcattcga
      301 ccaatgaagc tgataagcag aatgaagggg aacatgctag agaaaacaag ctagaaaagg
      361 cagaaggagt agcgatagca tctgaaactg cttcgccagc aagcaatgaa gctgcaacta
      421 ctgaaactgc agaagcagct agcgcagcta aaccagagga aaaagcaagt gaggtggttg
      481 cagaaacacc atctgcagaa gcaaaaccta agtctgacaa ggaaacagaa gcaaagcccg
      541 aagcaactaa ccaaggggat gagtctaaac cagcagaaga agctaataag actgaaaaag
      601 aagtccagcc agatgtccct aaaaatacag aaaaaacatt aaaaccaaag gaaatcaaat
      661 ttaattcttg ggaagaattg ttaaaatggg aaccaggtgc tcgtgaagat gatgctatta
      721 accgcggatc tgttgtcctc gcttcacgtc ggacaggtca tttagtcaat gaaaaagcta
      781 gcaaggaagc aaaagttcaa gccttatcaa acactaattc taaagcaaaa gaccatgctt
      841 ctgttggtgg agaagagttc aaggcctatg cttttgacta ttggcaatat ctagattcaa
      901 tggtcttctg ggaaggtctc gtaccaactc ctgacgttat tgatgcaggt caccgtaacg
      961 gggttcctgt atacggtaca ctcttcttca actggtctaa tagtattgca gatcaagaaa
     1021 gatttgctga agctttgaag caagacgcag atggtagctt cccaattgcc cgtaaattgg
     1081 tagacatggc caagtattat ggctatgatg gctatttcat caaccaagaa acaactggag
     1141 atttggttaa acctcttgga gaaaagatgc gccagtttat gctctatagc aaggaatatg
     1201 ctgctaaggt aaaccatcca atcaagtatt cttggtacga tgccatgacc tataactatg
     1261 gacgttacca tcaagatggt ttgggagaat acaactacca attcatgcaa ccagaaggag
     1321 ataaggttcc ggcagataac ttctttgcta actttaactg ggataaggct aaaaatgatt
     1381 acactattgc aactgccaac tggattggtc gtaatcctta tgatgtattt gcaggtttgg
     1441 aattgcaaca gggtggttcc tacaagacaa aggttaagtg gaatgacatt ttagacgaaa
     1501 atgggaaatt gcgcctttct cttggtttat ttgccccaga taccattaca agtttaggaa
     1561 aaactggtga agattatcat aaaaatgaag atatcttctt tacaggttat caaggagacc
     1621 ctactggcca aaaaccaggt gacaaagatt ggtatggtat tgctaaccta gttgcggacc
     1681 gtacgccagc ggtaggtaat acttttacta cttcttttaa tacaggtcat ggtaaaaaat
     1741 ggttcgtaga tggtaaggtt tctaaggatt ctgagtggaa ttatcgttca gtatcaggtg
     1801 ttcttccaac atggcgctgg tggcagactt caacagggga aaaacttcgt gcagaatatg
     1861 attttacaga tgcctataat ggcggaaatt cccttaaatt ctctggtgat gtagccggta
     1921 agacagatca ggatgtgaga ctttattcta ctaagttaga agtaactgag aagaccaaac
     1981 ttcgtgttgc ccacaaggga ggaaaaggtt ctaaagttta tatggcattc tctacaactc
     2041 cagactacaa attcgatgat gcagatgcat ggaaagagct aaccctttct gacaactgga
     2101 caaatgaaga atttgatctc agctcactag caggtaaaac catctatgca gtcaaactat
     2161 ttttcgagca tgaaggtgct gtaaaagatt atcagttcaa cctaggacaa ttaactatct
     2221 cggacaatca ccaagagcca caatcgccga caagcttttc tgtagtgaaa caatctctta
     2281 aaaatgccca agaagcggaa gcagttgtgc aatttaaagg caacaaggat gcagatttct
     2341 atgaagttta tgaaaaagat ggagacagct ggaaattact aactggctca tcttctacaa
     2401 ctatttatct accaaaagtt agccgctcag caagtgctca gggtacaact caagaactga
     2461 aggttgtagc agtcggtaaa aatggagttc gttcagaagc tgcaaccaca acctttgatt
     2521 ggggtatgac tgtaaaagat accagcctac caaaaccact agctgaaaat atcgttccag
     2581 gtgcaacagt tattgatagt actttcccta agactgaagg tggagaaggt attgaaggta
     2641 tgttgaacgg taccattact agcttgtcag ataaatggtc ttcagctcag ttgagtggta
     2701 gtgtggatat tcgtttgacc aagccacgta ccgttgttag atgggtcatg gatcatgcag
     2761 gagctggtgg tgagtctgtt aacgatggct tgatgaacac taaagacttt gacctttatt
     2821 ataaagatgc agatggtgag tggaagctag ctaaggaagt ccgtggtaac aaagcacacg
     2881 tgacagatat cactcttgat aaaccaatca ctgctcagga ctggcgcttg aatgttgtca
     2941 cttctgacaa tggaactcca tggaaggcta ttcgtatcta taactggaaa atgtatgaaa
     3001 agcttgatac tgagagtgtc aatattccga tggccaaggc tacagcccgt tctctaggca
     3061 ataacaaggt acaagttggc tttgcagatg taccggctgg agcaactatt accgtttatg
     3121 ataatccaaa ttctcaaact ccgctcgcaa ccttgaagag cgaagttgga ggagacctag
     3181 caagtgcacc attggatttg acaaatcaat ctggtcttct ttattatcgt acccagttgc
     3241 caggcaagga aattagtaat gtcctagcag tttccgttcc aaaagatgac agaagaatca
     3301 agtcagtcag cctagaaaca ggacctaaga aaacaagcta cgccgaaggg gaggatttgg
     3361 accttagagg tggtgttctt cgagttcagt atgaaggagg aactgaggac gaactcattc
     3421 gcctaactca cgcaggtgta tcagtatcag gttttgatac gcatcataag ggagaacaga
     3481 atcttactct ccaatatttg ggacaaccag taaatgctaa tttgtcagtg actgtcactg
     3541 gccaagatga agcaagtccg aaaactattt tgggaattga agtaagtcag gaaccgaaaa
     3601 aagattacct agttggtgat agcttagact tgtctgaagg acgctttgca gtggcttata
     3661 gcaatgacac catggaagaa cattccttta ctgatgaggg agttgaaatt tctggttacg
     3721 atgctcaaaa gactggtcgt caaaccttga cgcttcgtta ccaaggtcat gaagtcaact
     3781 ttgatgtttt ggtatctcca aaagcagcat tgaacgatga gtacctcaaa caaaaattag
     3841 cagaagttga agctgctaag aacaaggtgg tctataactt tgcttcacca gaagtaaaag
     3901 aagctttctt gaaagcaatt gaagcggccg aacaagtgtt gaaagaccat gaaactagca
     3961 cccaagatca agtcaatgac cgacttaata aattgacaga agctcataaa gctctgaatg
     4021 gtcaagagaa atttacggaa gaaaagacag agcttgatcg cttaacaggt gaggctcaag
     4081 aactcttggc tgccaaacca aaccatcctt caggttctgc cctagctccg cttcttgaga
     4141 aaaacaaggc cttggttgaa aaagtagatt tgagtccaga agagcttgca acagcgaaac
     4201 agagtctaaa atatctggtt gctttattga aagaagacaa gtcagcagtc ttttctgata
     4261 gtaaaacagg tgttgaagta cacttctcaa ataaagagaa gactgtcatc aagggtttga
     4321 aagtagagcg tgttcaagca agtgctgaag agaagaaata ctttgctgga gaagatgctc
     4381 atgtctttga aatagaaggt ttggatgaaa aaggtcaaga tgttgatctc tcttatgctt
     4441 ctattgtgaa aatcccaatt gaaaaagata agaaagttaa gaaagtattt ttcttacctg
     4501 aaggcaaaga ggcagtagaa ttgacttttg aacaaacgga tagtcatgtt atctttacag
     4561 catctcactt tactcattat gcctttgttt atgaatctgc tgaaaaacca caacctgcta
     4621 aaccagcacc acaaaacaca gtccttccaa aacctactta tcaaccggct tctgatcaac
     4681 aaaaggctcc taaattggaa gttcaagagg aaaaggttgc ctttcatcgt caagagcatg
     4741 aaaatgctga gatgctagtt ggggaacaac gagtcatcat acagggacga gatggactgt
     4801 taagacatgt ctttgaagtt gatgaaaacg gtcagcgtcg tcttcgttca acagaagtca
     4861 tccaagaagc gattccagaa attgttgaaa ttggaacaaa agtaaaaaca gtaccagcag
     4921 tagtagctac acaggaaaaa ccagctcaaa atacagcagt taaatcagaa gaagcaagca
     4981 aacaattgcc aaatacagga acagctgatg ctaatgaagc cctaatagca ggcttagcca
     5041 gccttggtct tgctagttta gccttgacct tgagacggaa aagagaagat aaagattaaa
     5101 tatcgaaaaa tcttgtgaaa tctttcctta tatttccaaa gtgtgatata atagtttcga
     5161 ataaaataaa taaaggagtt tttgtaacat ggcaaaactt actgttaaag acgttgactt
     5221 gaaaggtaaa aaagtcctcg ttcgtgttga cttcaacgta ccattgaaag atggcgtaat
     5281 cactaacgat aaccgtatca cagcagctct tccaactatt aagtacatca tcgaacaagg
     5341 tggacgtgca attcttttct ctcaccttgg acgtgtgaaa gaagaagctg ataaagctgg
     5401 taaatcactt gctcctgtag cagctgactt ggcagcaaaa cttggtcaag atgttgtttt
     5461 cccaggtgtc actcgtggtg ctgaattgga agcggcaatc aacgctcttg aagatggaca
     5521 agttctcttg gttgaaaaca ctcgttacga agatgttgac ggcaagaaag aatctaaaaa
     5581 cgatcctgaa cttggtaaat actgggcatc acttggagat ggtatcttcg taaacgatgc
     5641 attcggtaca gctcaccgtg cacacgcatc taacgttggt atctcagcaa acgttgaaaa
     5701 agcagttgct ggtttccttc ttgaaaacga aattgcctac atccaagaag cagttgaaac
     5761 tccagaacgt ccattcgtgg ctatccttgg tggttcaaaa gtttcagaca agatcggtgt
     5821 tatcgaaaac ttgcttgaaa aagctgataa agtccttatc ggtggtggga tgacttacac
     5881 attctacaaa gcacaaggta tcgaaatcgg taactcactt gtagaagaag acaaattgga
     5941 tgttgcgaaa gctcttcttg aaaaagcaaa tggtaaattg atcttgccag ttgactcaaa
     6001 agaagctaac gcatttgctg gttacactga agtgcgtgac actgaaggtg aagcagtttc
     6061 tgaaggcttc cttggtcttg acatcggtcc aaaatctatc gccaaatttg acgaagcttt
     6121 gactggtgcc aaaacagttg tatggaacgg acctatgggt gtatttgaaa acccagattt
     6181 ccaagctggt acaatcggtg tgatggacgc tatcgtgaaa caaccaggag ttaaatcaat
     6241 catcggtggt ggtgactcag ctgccgcagc gattaacctt ggccgtgcag acaagttctc
     6301 atggattagt acgggtggtg gagcatcaat ggaacttctt gaaggtaagg ttcttccagg
     6361 acttgcagcc ctgacagaaa aataagattt tataaacaaa tcaaagaaga gagggatgaa
     6421 agttcctctt ttcttttgct taaaataaaa acgcttcctc tcaactatta ctcataaaaa
     6481 tcaccgattt atgataaaat ggaaatagaa agttgagatt atgagttatt ttaaaaaata
     6541 taaattcgat aaatcccagt tcaaacttgg tatgcgaacc tttaaaacag gtattgctgt
     6601 ttttctagtt ctcttgattt ttggcttttt tggctggaaa ggtcttcaaa ttggtgcttt
     6661 gacagccgtt tttagcctga gggagagttt tgatgagagt gttcattttg ggacttcgcg
     6721 tattctagga aatagtatcg gtggactcta tgccttggtc tttttcttat taaatacctt
     6781 tttccacgaa gccttttggg tgaccttggt agttgttcca atctgcacca tgttaaccat
     6841 tatgacaaat gtagccatga ataacaaagc aggggttatt ggtggtgtag cagctatgtt
     6901 aatcattacc ctatcaattc caagtggtga gacaattttg tacgtgtttg tgcgtgtatt
     6961 agaaacgttt atgggagttt ttgtcgcaat tatcgtaaat tacgatattg atcgtattcg
     7021 tctcttttta gagaaaaaag aaaaataatg ttacatttta taacattatc aattgacgtt
     7081 tgtctttttt tagactataa tagacagaaa gaaggaaatt gtaaatgaag gaaaaagaat
     7141 ttcgccgaaa tatggctgtt tttcctatcg gcagtgttat gaagttgacc gatttatcgg
     7201 cgcgtcagat tcgttattat gaagatcaag agttgatcaa gcccgatcga aacgaaggaa
     7261 atcgtcgcat gtattccttg aatgacatgg atcgtctgct tgaaatcaaa gattatatct
     7321 ctgaaggtta taatatcgct gccattaaga aaaaatatgc tgaacgtgaa gcgaaatcca
     7381 agaaagcggt tagtcagact gaagtacgtc gtgcacttca caatgaactc ctccaacagg
     7441 ggcgttttgc ttcagtacag tcaccttttg gtcgcggtta ggcaaccgca agtagtcata
     7501 gattaaggag aaaacttatg ccaatcacag ctgcagatat tcgtcgtgaa gtcaaggaaa
     7561 aaaatgttac ctttattcgt cttatgttct cagatatttt gggaaccatg aaaaacgtcg
     7621 aaattcctgc tacagatgaa cagttagata aggtcttgtc gaacaaggtt atgtttgatg
     7681 gatcttctat tgaaggtttt gtacgtatca atgagtcgga tatgtacttg tacccggact
     7741 tggatacatg gacagtcttc ccttggggag atgaaaatgg aagtgttgca ggtctgatct
     7801 gtgatgtcta tacaacagaa ggtgaaccat ttgcgggtga ccctcgtggt aatttgaaac
     7861 gagctcttcg tcacatggaa gaagttggat tcaaatcctt caaccttggt ccagagccag
     7921 aattcttcct atttaagttg gatgaaaatg gggacccaac acttgaagtg aatgacaagg
     7981 gtggctactt tgatttggca cctactgacc ttgcggacaa cacacgtcgt gagattgtga
     8041 atgtcttgac caaaatggga tttgaagtag aagcgagtca ccacgaggtt gcggttggac
     8101 agcatgagat tgactttaag tacgatgaag ttctccgtgc ttgtgataag attcaaatct
     8161 ttaaacttgt tgttaaaacc attgctcgca aacacggact ttacgcaaca tttatggcga
     8221 agccaaaatt tggtattgct ggatcaggta tgcactgtaa tatgtccttg tttgatgcag
     8281 aaggaaataa cgccttcttt gatccaaatg atccaaaagg aatgcagttg tcagaaacag
     8341 cttaccattt cctaggcggt ttgatcaagc atgcttacaa ctatactgcc atcatgaacc
     8401 caacagttaa ctcatacaaa cgtttggttc caggttatga agcgcctgtt tacattgctt
     8461 gggctggtcg taaccgttcg ccacttgtgc gcgtacctgc ttcacgtggt atgggaactc
     8521 gtcttgagtt gcgttcagtg gatccaatgg cgaaccctta cgttgctatg gctgttcttt
     8581 tggaagttgg tttgtatggt attgaaaata aaatcgaagc accagctcct atcgaagaaa
     8641 atatctacat catgacagca gaagagcgca aggaagctgg tattacagac cttccatcaa
     8701 ctcttcacaa cgctttgaaa gctttgacag aagatgaagt ggttaaagct gctctcggag
     8761 atcacatcta cactagcttc cttgaagcca aacgaatcga atgggcaagt tatgcaacct
     8821 tcgtttcaca atgggaaatt gataattatt tagaccttta ctaatactaa tatagaagaa
     8881 agattgcctg tgggtaaaca gtcacacagc agtctaagcc atcaaatacc tataaccctt
     8941 aaaatagcga tttcggtcgc tatttatttt tcgaaaaatc ctcttgactc ttttgcgaat
     9001 tagatttatc atcagacgtg tagaagaaaa ttaaggagtc actatggaaa aattcaattt
     9061 caaaaacaat atcgggcaag aaaacaagtt acttcaaatc gaaatctaca agtttactaa
     9121 cttttgcaag cttcaaaatt acacatctgt aaatattttt tcgaaagata tatttgaaac
     9181 catagtaaat tgagactaga atagtacact acatctgcta aaaatttcta gaaactaatt
     9241 taactttcct aatcgatttg tttacatctt atttcaatct actatattat ctcaataaat
     9301 gatgatggcg actgttttgt ttgtgcttgt cgatttttga atcctgatcg agcggaagaa
     9361 tatttctgcc gaggttgcca aggggaaaat cctgaagata ttgaatttta tgatgaacaa
     9421 ttacaggcag aaaaggtgga ggatttgaat attcgattgg aagtaaaaaa ttgaattatc
     9481 tacaatctca tcaacaataa acctatttca accccattat ctataggcgt atttttacgc
     9541 ctttttatat acttgaagca cataagaaac attatgggaa gtttgctgat ggaagtactc
     9601 aagaaattga tgttccttat gatattcctg atacttggga gtgggtgagg ataaaatcaa
     9661 tttattggaa ttttgggcaa aataagccag agaaatcctt taggtatata gatacgtcta
     9721 gtattgatag aaaaaagaac ataatcaact acaaaaatct acaatatctt tcacctgaac
     9781 aagcgccttc ccgtgctaga aaattagttt cgcagaatag tgtcttattt tcaacagtta
     9841 gaccatatct aaaaaatatt gctgtagtta gagaacttaa agagtatttg atagctagta
     9901 cagcatttat tgttttggat actttactta acgaaacata tttgaaatat tatttattat
     9961 cagataattt tatcaatcgt gtta
//
LOCUS       DAVLWA010000071         9727 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLWA010000071 DAVLWA010000000
VERSION     DAVLWA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.071"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(47..1810)
                     /locus_tag="VYN80_001598"
     CDS             complement(47..1810)
                     /locus_tag="VYN80_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1541482.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASFFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            complement(1819..2409)
                     /locus_tag="VYN80_001599"
     CDS             complement(1819..2409)
                     /locus_tag="VYN80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1541483.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDFYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            complement(2507..2998)
                     /gene="rocS"
                     /locus_tag="VYN80_001600"
     CDS             complement(2507..2998)
                     /gene="rocS"
                     /locus_tag="VYN80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1541484.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            complement(3075..3767)
                     /locus_tag="VYN80_001601"
     CDS             complement(3075..3767)
                     /locus_tag="VYN80_001601"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1541485.1"
                     /translation="MKIGIIAAMPEELAYLVQHLYNVQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            complement(3784..4098)
                     /locus_tag="VYN80_001602"
     CDS             complement(3784..4098)
                     /locus_tag="VYN80_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1541486.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            complement(4110..4655)
                     /locus_tag="VYN80_001603"
     CDS             complement(4110..4655)
                     /locus_tag="VYN80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1541487.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            complement(4665..6044)
                     /gene="glmU"
                     /locus_tag="VYN80_001604"
     CDS             complement(4665..6044)
                     /gene="glmU"
                     /locus_tag="VYN80_001604"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1541488.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            6188..6988
                     /locus_tag="VYN80_001605"
     CDS             6188..6988
                     /locus_tag="VYN80_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1541489.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            7083..7925
                     /locus_tag="VYN80_001606"
     CDS             7083..7925
                     /locus_tag="VYN80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1541490.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGALAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLTWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            7925..8407
                     /locus_tag="VYN80_001607"
     CDS             7925..8407
                     /locus_tag="VYN80_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1541491.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            8407..9027
                     /locus_tag="VYN80_001608"
     CDS             8407..9027
                     /locus_tag="VYN80_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1541492.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(9281..9376)
                     /locus_tag="VYN80_001609"
                     /pseudo
     CDS             complement(9281..9376)
                     /locus_tag="VYN80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9656..>9727
                     /locus_tag="VYN80_001610"
     CDS             9656..>9727
                     /locus_tag="VYN80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541493.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKL"
BASE COUNT         2964 a         2063 c         1684 g         3016 t
ORIGIN      
        1 aatacttacc tatcatggga ggaacaaccg ttcctctttt ttattactaa aattcaaaga
       61 attccaatgc ttttttcaag agcaaatccg tatattctgg atcttcttgg gctacttcta
      121 tttcccgctg aactttttcc aaatcatctg taatcactcc atctactcct aagtgaagag
      181 atttgctgat agcttctgaa tcattgacag tccagacata aagtttctga tccgttgtcc
      241 atagtttgct tacaaaatat tcatccaagg ttgagtactc catagtatat cctgtcgctc
      301 ttgttttagg aaagacagaa ttgtagggca tgatgaaata aactggcagt tcggcatcat
      361 actgtcttac tttttcgaca acatggtagt ctaaagactg gatttgatgt ccataaatct
      421 tgagctttgc agcataacgg gctaaaaagc ggttcatcat gtctggacta tcttttttac
      481 tggttttaat ttcaattagt aatttttgac caagttcgtt ggctcgactg agataatctt
      541 caaagcttga aattttagtc tggtagccat tttcaaaaat atcaatccct ttaagctcct
      601 ccaagtttaa gtcttgagga cttttattga tacctgctag atttttcaag ttagcatcat
      661 gcatcatgac aaactgccca tcttttgttt cctgcacgtc cgtctccacc aagtctggtt
      721 tgagttgtgc tgtagtttcc aaggactcta ctgtattttg aatcccattt gcattggaaa
      781 cccctcggtg agaaataagt tgaggtagat gaaccatggg agcctccaga taaatataac
      841 cttctaaggc aaagaaaaaa ctggcacaag tcatgacacc ccatcgcacg atgtgatctt
      901 tttctctcct aggaagcata tccagctcct ttcctgtcaa aaatgaaaca aatttaacca
      961 aaaaataagt cagagccata taatagagat ttttaatcac gacaaaattc aaaataccaa
     1021 gaatcagaga ctctctctga gtgatatcat ctaccaaagt ttgagccaat aataaaggaa
     1081 tcaaaggaag ataaaataat aaatgtgctt tgagcaagat gtaaaataaa ttccaagcat
     1141 aaaaagtaac tctcttcttg gttttctcca agctaaacat cactgcttct cgaacagtca
     1201 gctgatcata tacaatcttc ggaagggcaa acatcaatct gacagagaca tagagaaaga
     1261 taagagatag aagtaggatg ctcagccacc acatccaata tttatcttct aaataagctt
     1321 ggataaactc tggaatgacg attttattaa gataataaat cttcagcatt ttccgtataa
     1381 aaggaaacag catagctata tagaaaaaga taaacaaggc tttagcgcca gttagctttt
     1441 tcataaattc aaaactttca tggaaaacct tgcggatata ctcaattagc cttcgctttt
     1501 cattatagag gagatgacga gcaccaataa agaggagtcc tatttgaaaa taagcaacca
     1561 gaaagttaat tacaatcaag gctaaaaaag ctagactaat caatggagaa tgagtaagga
     1621 tggctaagac attgttatag gaaataaaaa gataacctgt ctgatctaat aagaagctag
     1681 ccaaccatga attgaatggt acccacaaat actccactat cataaaaatc aagaaaaata
     1741 gaaagaggat tttatcaaga ttgaggtaaa tctgtttaag acccaatttt ttaggttttt
     1801 caggtttcat aggcactcct agtcaaataa ttgagacaag tccaagccac caaaaggatt
     1861 gtttgataag ctactttctg tctctaacaa ttccctagct tgatccgact ctaagaagga
     1921 ttcgtaaaca cgcgccgtca tccgagcatc ctctaaacta ttatgagact gaccttgaaa
     1981 tccaagaaat gaggcaacag tttgcaattt gagattggca ataccatgta aatctgaact
     2041 ccgacgttca aaagcttcat catacaaatc caccttgtac tgttggctat agtctaaacc
     2101 atgctctgct aaaataggta aatcactttt agcagcattg tagcctatca tcagcaaaga
     2161 acccacaaac tcttggaatt cttctaagac tttctcaact ggaggagcat cttttaaggt
     2221 ctcagctgta atcccggtca aaccattgat aaagcttttt agagggacgc tggtatgaac
     2281 atagaaatca taggcatcga tttcccgacc atctctaaaa cgcactgccg atacttgaat
     2341 caagtgtgtt tgtccttcgt gctgattaaa ttctaaatca aaagcaatgt aatcttctaa
     2401 tcgttccatt ctgtacctct cctctactgt ctctgttcta atcaactata ctattcacaa
     2461 acaaaagaag ccatcaggtt agcatttaac ctaaacagct ccttgattat cctccaaata
     2521 aacgagcaaa aaagcctttt ttagtggatt ggacttcttc ttttgcttgg tccagctcta
     2581 gcttaagatt ttcttgatcc ttcatggctt gaagggtcaa ttgttgttgc tgatctaatt
     2641 gtttgtcttt ctcagcaatc tgacggtctt tgatacgcat ctgctcgtct ttttctgata
     2701 actgacgatc cttggctttt aattgttcat aaagacgaag aatttctgca ttcttctcat
     2761 ccactagaat ctccatcagt tcacgttgtt tgacatcttc actgacaggt tcatcttcaa
     2821 aaatcgtttt tttatagatt tcttctagct taatcaagcc acttctggta acaactgtta
     2881 cccctttgtc atttttatct gtgtcttctt ctggtaattc tttgacacgg ttattgattg
     2941 cttggcgaga taatcctaag acctctgcaa tctcactgac ggtcatttca atactcataa
     3001 tatcctctga aacgttttct agcttttctt tacttaaatc ttatcataag ctagaaaaac
     3061 tgtcaaattt ccgcttaatc taaagccttc aaaaaggcca acaagacttg ggcagagcga
     3121 cgtccagctt cgataataaa ctcatcaaaa aagatgtttg cttcatggtt ggcattgtca
     3181 ctcatagctc ggatgactaa gactgggaga ttgagggtat gcgctgcttg agcaatagct
     3241 gccccctcca tctccacggc taaaacttct gggaaatggg acttaatcgc ttctatcttg
     3301 tcatttcctg caacaaaact atctcctgta gcaatcaaac caagatgcca gttttggtcc
     3361 aattgagata aactctcttg gatttgagca acaaaggttt tgtctgattc gaaataaagc
     3421 ggttgttgcg ccatttgtcc ataagcatag ccaaaagctg tgacatccac gtcatgatag
     3481 gctaatttgt cagcaatcac gacatcccca acagcgatac cttctgctac tgccccagct
     3541 gaacccgtat taataagggc atccacctgg aaatgatcag ccaaaatcgc cacactcata
     3601 gcagacatga ccttaccaat tccactttct acaagaacga cttcatgaga agcaatggtt
     3661 cctgtatgat aggtattccc caaaacaact tgctcctgga cattatataa atgctggacc
     3721 agataagcca gttcttctgg catagcagca ataattccta ttttcatttc aattcctttt
     3781 ctattacaaa agtttcattg ctaaaacaag caaaatcaag agaaagatga ccgcgaataa
     3841 gattttattc aacttagaat tgaagacatt tctcttggta ttttcaatgc gacggctttt
     3901 atgaatagat ggttcgacct gaatcttcaa ggtttcctgg ctaaaaccat gattcttagg
     3961 attggcataa ccaaaacggg aagaagaggt tggtaaaatc ttagtctcct cattatcatc
     4021 tagcaaagga ggacctgaaa ttttttcgcc tctattagct ctttcaatca tttcatccgt
     4081 taataaagat ttacccatac tgacctcctc tattttttct gcaactccca atactgaaca
     4141 gccataattg tcttggcatc acaaatatga cctgattgga ttaattcttt cgcttcttct
     4201 aagctcactt caaggacttc caaggtttca tcctcatcct gcggacgcgg attttccact
     4261 tttgtcaaat cgcttgctaa atatagtttt aacttctcat tacaaaagcc aatagctgaa
     4321 taaaaatcgt acaagagttc taatttccct gtataggctg tttcttcctc taattcacga
     4381 agggcagctg ccacaggggc tgtgttttct cctacttcca attttccggc tggaatttcg
     4441 taagagacag cctcgatagc tttgcggtac tgcttgacca agatcagttt ttgttcatcc
     4501 gttactgcta aaacacagac agccccattg tggaaaatca aatcccgttg ggcagttccc
     4561 ttgccttctg gtaattcaac ctgatcttgg accagtttaa atattggtcc ttgatagatt
     4621 tcttttcggc taagcgtttt ttcttcaaac tccatgatag gctcctactg gttcttagga
     4681 tgatgaggaa gacgtgttgc atattcgtct ttattgatct gacgaccgcg accaatagca
     4741 atagcatctg ctggcacgtc tttagtaata gttgaaccag caccaacgag ggaattgtca
     4801 ccaagttcta ctggtgcaat aatggttgaa tttgaaccaa caaagacatt gtctccaatg
     4861 actgtcttgt atttgttttt gccgtcataa ttgactgtaa tagttccagc accgaaatta
     4921 acgttgcttc ccacttcaca gtttccgata taagtcaaat gaccagcctt ggtattctca
     4981 ccgattgaag atcctttcac ctcaacaaag ttaccaatat gaacttgggc acccagactt
     5041 gaatttggac gaatgtgagc ataaggaccg actgtcacac cgtctgcaac actactttcc
     5101 tcaatcatag aattggtaat gaccgctcct gctccgatag tgctgtccac tacataagta
     5161 ccgtttgtca aaacagtctc agcaccaatt ttcgtttgcc ctttcaaggt aacattggct
     5221 tcgatttgaa cttccggagc aatctcaaca tcaatatcga tataagttgc ttctggattg
     5281 acaaagctaa caccgttgac catatgttta tgattgatgc gacgacgcat aactgactca
     5341 gctgtcgcaa gcgccacacg gtcatttacc ccaagacttt catcaaaatc tttcaaagta
     5401 taagcgccaa ctttttcacc agtttcacgg aaaataccaa tgacgtctgt aatatagtat
     5461 tcgccttgag cgttattggt attgatattt ttcaaagcct caaacaaacg ctcgttgtca
     5521 aagacgtatg ttccagtgtt gatttccttg atttgctttt caaaatctgt agcatccttc
     5581 tgctcaacaa tacgaagaac ctcagcattg tcattacgaa caattcgtcc atagccaaaa
     5641 ggattatccg tttcagcagt caagatagtg gccacatttt tatgattgat atggaaatca
     5701 atcaagtttt tcaagctttc accagtgatt aaaggagtat ctcctgcaat gaccaaggtg
     5761 tgtcctgaca aaccttctaa gataggttct gtcatcataa ctgcatgacc agttcccaac
     5821 tgttcagatt gagtcacaaa ttctgtctgt ccagccaaga cctcctcaac caattctgcc
     5881 ttgtgtccta caactgttac tgtcttttca ggttggatag ctcccacact acggaaaaca
     5941 tgttccaaca tagaaatacc cgcaaccttg tgcaaaactt ttggcaaatc agatttcatg
     6001 cgagtccctt tacccgctgc taaaataatg gcaaaatttg acataatctt ctcttttctg
     6061 tagaaattcc cttttattat accatatttc aaatcattcc gcactcttga atgaaaaaat
     6121 gctctaaagt ctcttcctta acccattttt ttgatttttt ttgattttta gggtagaatt
     6181 ataagatatg agaaaacaaa ttcatccact tattttattt agttttttgg ggattatgat
     6241 gttcatttta atcatcaatc gcccacttaa tgacaaccaa tcgtttaaaa caaaatccaa
     6301 tatagcacag attgaagcac aggctttgaa acacttagac aaaccgatta ttgacctctc
     6361 tggctggcag cgtcctgaag aaatcaacta cgatgccctc tcacaaaata tttcaggtgc
     6421 tattgttcgt gttcacagtg gtgctcaaac gacaaaagaa aatgatgctt catttatcaa
     6481 tggaatagac aaggcctata agagtcatat cactgaactt caaaagcgga atgtcccagt
     6541 tgctgtctac gcttatgtag ctggaaaaag tgtccaagaa atggaaaagg ctgccgaagt
     6601 tttctacaat gctgcctctc cttacagccc tagttactat tggcttgacg tggaagacaa
     6661 aaccatgtct aatatgaacg aaggtgtcga aaactttcga gcaaaactag catcactagg
     6721 tgctaaaaac atcggcatct acgttggtgt ctacttcatg gaagaacaca gtattgatac
     6781 aggcaagttt acctctgttt ggattccgtc ctacggctca gactcaggat ttttggagag
     6841 cagtcctaaa actgatttag attatgatat tcaccaatac acatctaaag gaaaaattgc
     6901 cggctttgac cacgatttag atatcaacgt tatctctccc ttaaaaaata aagaagaaac
     6961 ctttcgaaaa ctttttttaa aaccttaaaa gcatttgttt atcatttgct ttttcttttt
     7021 gtgtctgatt gtgatacaat atagtaagga ttttttgaaa tagaaatagt tggaggaaat
     7081 atatgctctc atggttagca cgcgttatta aagggattgt aattgctctt ggatttatcc
     7141 taccgggaat ttccggaggg gctctagcag caatcttagg aatctatgaa cgaatgattg
     7201 gctttctggc ccatcccttt aaagacttta aagaaaatgt tttgtacttt attccagttg
     7261 ccatcggtat gcttctagga atcggcttat tttcctaccc gattgaatac ctgcttgaaa
     7321 attatcaggt ctttgtatta tggagctttg cgggagctat tatcggtaca gttcctagcc
     7381 tcctcaaaga atcaactcga gaatctgacc gagacaagat tgatttaact tggttatgga
     7441 caacctttat catttctgga ttaggactct atgccttaaa ttttgtcgtt ggaaccttaa
     7501 gcgccagctt tcttaacttc gtcctagcag gcgcactatt ggcccttggc gtcttggttc
     7561 ctggcctcag cccatcaaat ttacttttga ttttgggact ctatgctcct atgttgactg
     7621 gttttaaaac ttttgatctc ttgggaacct tctttccgat tggaattggt gcaggtgcaa
     7681 ctctcatcgt tttttcaaaa ttgatggatt atgccttaaa caactaccac tcacgcgtct
     7741 atcatttcat catcggtatc gtcctatcaa gtaccctttt gatcttaatt ccaaatgcag
     7801 gaaacgctga aagtatccaa tacacaggac tttcacttgt cggttatgtc atcatcgcct
     7861 tcttctttgc gctgggaatc tggcttggta tttggatgag tcaattggag gataaatata
     7921 aataatggca aaaaaagtta aaatcaaaaa aacattggtg gaacaaatcc tatctaaagc
     7981 agctatccct catcagggaa ttcaaatcaa tgccctagaa ggagagcttc ctcaaggtta
     8041 tgaacgagat cagattttca aaaccttggc gcttttggga gacaagaccg gaccgattat
     8101 cggaattgtc cctatcactc agcacttgtc tgaaaagaaa ctagctaaaa tttctggcaa
     8161 taaaaaagtg agcatgattc cacaaaagga cttagaaaaa acaactggtt acattcatgg
     8221 agccaataat cctgtcggaa ttcgtcagaa acacaattat cccattttta tcgataaaat
     8281 tgctctagat ttggatcaaa tgattgtctc tgcaggcgaa gtcggacaca gcattatcgt
     8341 cgcgccacaa gacttggcta gctttgtaaa ggcagacttt gtagatattt tggaggacat
     8401 caagtaatga aactctactt tgtccgccac ggtcgtaccc tctggaacca agaaggtcgt
     8461 tttcaaggtg ctagtggtga ttctcctctt cttcctgaat ccattgaaac cctaaaacga
     8521 cttggtcagt atctcaagga aattcctttt gatcagattt attcaagtga tttacctcga
     8581 gcggtcaaat ctgctgagat tatccaaagt caactctata ccccctgttc tttagaaatc
     8641 gttcctaatc tccgtgaatg gcaacttggg aagttagaag gtttgaaaat tgcaaccttg
     8701 gaagctattt acccgcaaca aatccaggct tttcgttcaa atcttgctca atttgacact
     8761 cgaatgttcg gagccgaatc cctctatagt acaactcaac ggaccatcca atttatcaaa
     8821 tcattaaaag atagtccagc tgaacgtatt ctaattgtcg gacacggcgc caatcttact
     8881 gccagtcttc gtactctcct aggctataaa gaaccacttc ttcgtaaaga tggcggtcta
     8941 gcaaatgcca gcctgaccat tatagaaacc catgattttg aaacattcac tctcaatact
     9001 tggaatgata cttcttatca atcataatta tagcctagcc ttctgaacta gaaccatagg
     9061 agatatagtc agttgaaaca aaaataagat aaaagagcct cgtaaaaagg tattgcaact
     9121 tgccaatacc ttttttgata tgaacacgtg tcttctcaac aggattgtag agcaaaatga
     9181 aataagaact gaataatatg atttgagaat ttaaatcaat ttataagaat gttttagaag
     9241 cagttatgta ctattttaga ttcacttttc tatactaaga tgagtaggga ggaaaaggta
     9301 aaagtttatg cccaaactct tcacataaga actctagctt acccattcta tggaatcttg
     9361 cattatccat taaaacttcc agactcatac ccttctaaaa tctattcaaa ccacgtcagc
     9421 ttcacattat atatgttggt tacctcacaa gttttatctg caacctcaaa gctgttcttt
     9481 gagcagccta cagctagctt cctagtttaa tctttgcttt tcactgagta tcaagttaaa
     9541 aaatgaggct ggtcaaaaag tctttaaaat aaaaaaaacg catattatca ggtgttaaat
     9601 gaactgcacc ccaaaagtta gacagaaaaa atctaacttt tggagtgttt ttattatgaa
     9661 attaacttat gatgataaag ttcagatcta tgaacttaga aaacaaggat atagcttaga
     9721 gaagctt
//
LOCUS       DAVLWA010000072         9250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLWA010000072 DAVLWA010000000
VERSION     DAVLWA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.072"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            434..1909
                     /locus_tag="VYN80_001611"
     CDS             434..1909
                     /locus_tag="VYN80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1541494.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1875..2590)
                     /locus_tag="VYN80_001612"
                     /pseudo
     CDS             complement(1875..2590)
                     /locus_tag="VYN80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2846..3706
                     /gene="speE"
                     /locus_tag="VYN80_001613"
     CDS             2846..3706
                     /gene="speE"
                     /locus_tag="VYN80_001613"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1541495.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            3703..4962
                     /locus_tag="VYN80_001614"
     CDS             3703..4962
                     /locus_tag="VYN80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1541496.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4962..6089
                     /gene="nspC"
                     /locus_tag="VYN80_001615"
     CDS             4962..6089
                     /gene="nspC"
                     /locus_tag="VYN80_001615"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1541497.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6086..7171
                     /gene="aguA"
                     /locus_tag="VYN80_001616"
     CDS             6086..7171
                     /gene="aguA"
                     /locus_tag="VYN80_001616"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1541498.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7181..8056
                     /gene="aguB"
                     /locus_tag="VYN80_001617"
     CDS             7181..8056
                     /gene="aguB"
                     /locus_tag="VYN80_001617"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1541499.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8237..9046)
                     /locus_tag="VYN80_001618"
     CDS             complement(8237..9046)
                     /locus_tag="VYN80_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1541500.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2713 a         1775 c         2076 g         2686 t
ORIGIN      
        1 aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa aatgagatga
       61 tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag ggttgaatgc
      121 cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt tataagttga
      181 tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca cgtcatcttg
      241 tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac atcgggaaca
      301 gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag gtgaaaacct
      361 gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct aaaaatattg
      421 aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg aggccttggt
      481 gaagttacgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc gtggacgggg
      541 aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg tcaattcgat
      601 gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag aggagctggc
      661 tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa cttcatcggt
      721 gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc cacgaaatgt
      781 ccataaatct gctatcaatg cgttggttct atgtggtgcc attcccatct atatcgagat
      841 gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag tagcacaggc
      901 cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt actacggcat
      961 ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga tggttttagt
     1021 agatgaagcc catggagcgc atttgcattt cactggtaaa cttccaattt ctgctatgga
     1081 tgcaggagct gatatggcag cagtttccat gcataagtct ggtgggagtt tgacacaaag
     1141 ttcgcttctt ttaatcgggg agcagatgaa ttctgaatac gttcgtcaga taattaacct
     1201 gactcagtct acatctgcct cttacttgtt gatggctagt ttggatattt cacgtcgcaa
     1261 cttggccctt cgtggtaaag agtcgtttga gaaagtcatt gagctatctg agtatgcccg
     1321 ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa tagacggtgt
     1381 ttcggtttgc gattttgacg taactaagct gtcagtttac actcagggta ttggcttaac
     1441 aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg agtttggtga
     1501 tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca tcgagcgctt
     1561 ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag atttgatagc
     1621 aggagaatat attcagcccg agttggtgct gtctccacaa gaagccttct attcagagag
     1681 aaaaagtttg accttagatg agtctgttgg acaggtctgt ggagaatttg tcatgtgcta
     1741 tcctccaggg attcctatct tggctcctgg tgaacgcatt acacgagaaa ttgtagacta
     1801 tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc cagaggtcaa
     1861 tcatatcaac gttattaaga gaaagacaaa ctataagaaa agtcaatagt tttatctaaa
     1921 ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca aaaagccctt
     1981 gaattagaag cggtcaatcg cttaatttct atcagcttat caaatcctgc ctcaatcctt
     2041 ttctgaggat tagggtagcg tgtcaagagt tggtaggtat attctgaatg ctttccaacg
     2101 attttatcca actcaggaaa gatgatatca agacaacgag tgtattgtac tttccaatca
     2161 gactgttttt cttgagacga tgaatatgtc tagccagtat ttttagttct acttgccgat
     2221 tatcgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg atgccatgag
     2281 cgtctttctt atccgtttta gtcttgtgaa gtgataatga tttggcaaat tccttgatga
     2341 gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc agtagattaa
     2401 aggcataatg tccggtattt tcaagagcga tgagacagtc ttggttgagt tgtcgaagag
     2461 acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt ttaagaacag
     2521 tttttcctgg aacattcaag gctgtaacat cgtgtttatt tttagcgaca tcaatgccca
     2581 cataaagcat gggagtacct ccagatgtag tatttcaagt ctacttggtt atccacgaat
     2641 tttttgcctt gttaccttag acgagataaa acgtctatgc gttatcaaac tcattaccaa
     2701 ttgaaacaaa aaactgtggt tagagccttt cggaaatcgt caagcgattg gagggaataa
     2761 actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta gctaactgcg
     2821 ctaaatataa tataaggaga aatagatgga tttatggttt tctgaagttc atactccaga
     2881 tgtcaaattg tctctgagaa cagccaagca actttacgct ggaaaaagtg aatggcagga
     2941 tatcgaagtc ttggatacgc cagcttttgg gaaaatactg attttaaatg gccatgtctt
     3001 gttctcagat gcggatgatt tcgtctacaa tgaaatgacc gttcacgttc ccatggctgt
     3061 ccacccaaat ccaaagaaag tattggttat tgggggtggt gacggcggtg ttgcccaagt
     3121 attaacactc tatcctgaac tggaacaaat cgatatcgtg gaaccggatg agatgttggt
     3181 cgaggtctgt cgtgagtatt tcccagactt tgctgcaggg ctagatgatc ctcgtgttac
     3241 catttactac caaaatgggc tacgcttttt gcgaaactgc gaagatgatt acgatattat
     3301 catcaacgat gcgacagatc catttggcca tacggaagga ctctttacca aggaattcta
     3361 cggcaatagt tatcgagctc tgaaggaaga cggcatcatg atttaccagc atgggagtcc
     3421 cttctttgac gaggatgagt cggcctgccg aagcatgcac cgcaaggtca atcaagcctt
     3481 tccaatcagt cgggtttatc aggcccatat tccaactagc ccagctggct attggttgtt
     3541 tggatttgca tcgaaaaaat accaccctgt caaagatttt gacaaggaag gttggaaaaa
     3601 acgccagctt ttcacagaat actacactgc aaacttacac gtgggatcct ttatgttgcc
     3661 caagtatgtt gaggacattt tagaagaaga ggaaggaaaa aaatgagtcg tttactagtt
     3721 attggttgtg ggggcgttgc ccaagttgct atttcaaaga tttgtcaaga tagcgaaaca
     3781 tttacagaga ttatgattgc tagccgtacc aagtcaaaat gcgatgactt gaaagcgaag
     3841 ctagaaggca aaacaagtac taaaattgaa actgcagcac ttgatgctga caaggttgaa
     3901 gaagtgattg ccctgattga aagctacaaa ccagaagctg ttttgaatgt agctctgcct
     3961 tatcaagatt taaccattat ggatgcttgt ttggcaacag gtgttcacta tatcgataca
     4021 gccaactacg aagcagaaga cacagaagac cctgagtggc gtgctatcta cgaaaaacgt
     4081 tgtaaggaac ttggttttac agcctacttt gactactcat ggcagtgggc ttatcaagag
     4141 aaattcaaag aagcaggctt gactgctctt cttggttctg gttttgaccc aggtgtaact
     4201 agtgtctttt cagcttatgc cctcaaacac tattttgatg aaatccatta tatcgacatt
     4261 ttagactgta atggcggtga ccacggttat ccatttgcaa ccaactttaa tccagaaatt
     4321 aatctccgtg aggtttctgc gccaggttct tactgggaag atgggaaatg ggtcgaagtc
     4381 gaagctatgt ctatcaagcg tgagtatgat ttccctcaag ttggacaaaa agatatgtat
     4441 ctccttcacc atgaagaaat cgaatcattg gccaagaaca ttccaggtgt caaacgcatt
     4501 cgtttcttta tgacttttgg tcaatcttac ttgacgcaca tgaaatgtct tgaaaatgtt
     4561 ggactccttc gtacggatac cattaacttt aacggccaag aaattgttcc aattcaattt
     4621 ttgaaagcct tgcttccaga tcctgctagc cttggcccac gtactgtagg taaaaccaat
     4681 attggatgta tctttacagg tgtcaaagat ggcgttgaaa agactatcta catctacaat
     4741 gtttgcgacc atcaagagtg ctatgcagaa gtcgggtcac aagcaatttc atacacgaca
     4801 ggcgttccag ccatgattgg gacaaaatta gtcatgaacg gaacttggaa acaagctgga
     4861 gtgtataacc ttgaggagtt agatccagat ccattcatgg aagctttgaa tgagtatggt
     4921 ttgccatggg ttgtggttga aaatccacaa atggtggact aatgaagtta gaacaagtac
     4981 caacaccagc ctatgttatt gacttggcca agttagaagc taattgccgc attctacaat
     5041 atgtacaaga agaggccggt tgcaaggtct tgcttgccca gaaggcatat tccctctaca
     5101 aaacttatcc cttgattagc cagtatctat caggtacgac agctagtgga ctctatgagg
     5161 ccaaattggc aagggaagaa tttcctggtg aagtccatgt ttttgcacca gctttcaagg
     5221 atgaggacat ggaggaattg cttgggataa cagaccatat cgtctttaac tcagagagac
     5281 agttgcgtaa gcatggggct cgttgtcgag aggctggtgt cagtgttggt ttgcgcctca
     5341 accctcagtg ttcaactcaa ggagatcacg cgctctatga cccttgtgca cctggttctc
     5401 gctttggagt tactatagac aagattccga gtgatttgct agatttggtt gacggacttc
     5461 attttcatac cctttgcgag cagggagcag atgatttaca aacaactttg aaagcagtag
     5521 aagaacagtt tggtccctac ttacatgagg taaaatggct caatatgggt ggtggtcatc
     5581 atattacaag agaaggttac gatgtggatt tgctgatttc agaaatcaag cgtatccgaa
     5641 aaacttacaa tcttgaaatc tatatcgagc ctggtgaagc cattgcgctt aatgcgggtt
     5701 atttagcaac tgaggtatta gatattgtag aaaacggtat ggaaatcttg gttttagacg
     5761 cctctgcgac ctgccatatg cctgatgtac ttgagatgcc ctatcgtcca cctttgagaa
     5821 atggctttga gtcacaggaa aaagcccata cctacagact ttcttctaat acctgtctga
     5881 ctggcgatgt gattggtgat tatagttttg aaaatccagt ccaaatcgga gacagacttt
     5941 attttcaaga catggccatt tattcttttg tcaaaaataa tacctttaat ggtattggat
     6001 tgccaagtct ctatctcatg gacgaacagg gagactgtag cttactcaaa gcttttggct
     6061 atcaagactt taaagggaga ttatcatgat ggacagtcca aaaaaattag gctatcacat
     6121 gccagcagag tacgaacccc atcatggtac cctcatgata tggccgactc gaccaggatc
     6181 atggcctttt caaggaaagg ctgctaaaag agcatttact cagatcatcg agaccatagc
     6241 agaaggggaa agagtctatc ttttggtgga gcaggcctat ctatctgaag cccaatccta
     6301 tcttggagac aaggttgttt atttagacat tcccaccaat gatgcctggg cgcgtgatac
     6361 tggcccaacc attctcgtca atgataaagg taagaaatta gccgtggatt gggcctttaa
     6421 tgcttgggga ggcacctatg atggtcttta tcaagattat gaagaggatg accaagtagc
     6481 cagtcgtttt gctgaggcct tggaaaggcc tgtctatgat gctaaacctt ttgtactgga
     6541 aggaggcgca atccatagcg atggtcaagg aactattctc gtaactgaaa gttgcttgct
     6601 tagtcctggt cgcaatccta acctgactaa agaggagatt gaaaacacat tattagaaag
     6661 tcttggtgct gaaaaagtta tttggcttcc ttatggtatt tatcaggatg aaaccaatga
     6721 acacgtcgat aatgttgttg cctttgttgg tcctgctgag gttgttttgg cttggacaga
     6781 tgacgaaaat gatccccagt atgccatgtc aaaagcagat ctcgaactct tagaacagga
     6841 aacagatgca aaaggttgtc acttcaccat tcataaattg cctatccctg cagttcgaca
     6901 agttgtgaca gaagaagatt tgccaggcta catctatgaa gaaggagaag aagagcgata
     6961 cgcaggtgaa cgactagcag cttcctacgt aaacttttat atcgccaaca aggctgtctt
     7021 ggttccacag tttgaggatg taaacgacca agtggcctta gatatcctca gcaagtgttt
     7081 cccagaccgt aaagttgtcg gaataccagc cagagatatt ctcttaggtg gtggcaatat
     7141 ccactgtatc acccaacaaa ttccagaata ggagaaaaag atgagaaatg taagagttgc
     7201 aaccattcag atgcaatgcg ctaaggatgt ggcaacaaat atccaaaccg cagagcgttt
     7261 agtacgtcag gctgctgagc aaggagccca aattattctc ttgcccgagt tgtttgaaca
     7321 tccctatttc tgtcaggaac gtcagtatga ctactaccag tatgcccaat ctgtagcgga
     7381 aaatactgcc attcagcatt ttaaggtgat tgctaaggaa ctacaagttg ttttaccaat
     7441 cagtttctat gaaaaagatg gtaatgtctt gtataactct attgccgtca ttgatgcaga
     7501 tgggggagtg ctgggcgttt atcgaaagac ccatatacca gatgaccatt attatcaaga
     7561 aaaattctat ttcacgcctg gtaacactgg tttcaaggtc tggaatactc gctatgctaa
     7621 gattggtatc ggtatctgtt gggatcaatg gttccctgaa acagcccgct gtcttgcatt
     7681 gaatggtgct gaattgctct tttatcctac agctatcggt tcagagccaa ttttggatac
     7741 agatagttgt ggtcactggc aacgtactat gcaagggcac gcagcagcga atattgttcc
     7801 agtcatcgca gccaatcgtt atggtttaga ggaggttact cctagtgagg aaaatggcgg
     7861 acagagttcc agtcttgact tctacggttc ctcctttatg acggatgaaa caggagctat
     7921 tctagaacga gctgaaagac aagaagaagc tgttctgtta gctacttatg acctagacaa
     7981 gggagcaagt gaacgtctaa actggggctt gtttcgagat agaagaccag aaatgtatag
     8041 acaaattaca gattagtgtg ggagaaatga gagattcatt ctgctagact aacttcttat
     8101 tagcaactat aagatactat ggcatctagt aaatcgattt ttatgattcg ctattcttgt
     8161 ctattgatta gtccgtattt taaaatatta gcaaaaaagc aaatagcagt aacttctgtc
     8221 tatttgcttt tcttttttat agaatatatt tctcaatagc acgcgcaacg ccgtcttctt
     8281 cgttgcttga ggtaacggca tccgcaagag atttgacata atcgctggca tttcccattg
     8341 caatcccaag ccctgcaaac tggagcattt cgatatcgtt attagcatcg cccatggcca
     8401 taatctctga ggaatcaatc ttcaaaatct cagctagtcg tgaaagagca gtagcctttg
     8461 tcgttccaag cggcattgct tcataaatga caggctgcga acgaactcca ctgaatcgtt
     8521 ggcaaagctc ttcagcaaaa cgctgctcaa aatcgtctgt ttgttctttt gttcctaaaa
     8581 acataccttg gaacatccgg aactttccac tagtcgcttc ttcaagagaa atttcagtca
     8641 ggtctgaaaa tactagttta gcatcatttt caataacttg attgggtttg ccaccgagaa
     8701 caaaataatg tgactcgtca aaaagtgtca actgaacatc actcttttca gcaaggtcat
     8761 agaggtattc gatgtcagct ggactcagtt ctttccagtc aactagactc caatcactgg
     8821 tctggtgagt tgaacaaccg ttgttaacaa taatatattc gttctggagg tcaagctcca
     8881 gttttttgta gtaggggagg acaccgaaaa gggggcgacc cgtacagaga accagtttga
     8941 cacctttttc aatggctttg tgaatagcag taatgtgagc ttgtgggatt tccttggctt
     9001 cattgaggag ggtgccgtcc atatccaagg ctagtagttt aatcataggt cttccttctt
     9061 tatcttttgc tattattata gcatattttg gagaagaaat tgatagaaag cttgagacta
     9121 attgatttta tagtttaaga tgttttgatg acaattcatg atttgaagag gatatttggc
     9181 aaagatatgc tatactatgt ttgtcaatgt tgcaactaga caaattaaaa aaccaactta
     9241 atataatagt
//
LOCUS       DAVLWA010000073         9187 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLWA010000073 DAVLWA010000000
VERSION     DAVLWA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.073"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..213
                     /locus_tag="VYN80_001619"
     CDS             <1..213
                     /locus_tag="VYN80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541501.1"
                     /translation="NNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFADFVVQV
                     DKSQSQLAIQKSLEELETLKKSLMQE"
     gene            282..518
                     /locus_tag="VYN80_001620"
     CDS             282..518
                     /locus_tag="VYN80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1541502.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            515..928
                     /locus_tag="VYN80_001621"
     CDS             515..928
                     /locus_tag="VYN80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1541503.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1046..2011
                     /locus_tag="VYN80_001622"
     CDS             1046..2011
                     /locus_tag="VYN80_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1541504.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1977..2444
                     /locus_tag="VYN80_001623"
     CDS             1977..2444
                     /locus_tag="VYN80_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541505.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGENKIFESIDNLFDIIDGDRGKN
                     YPKSDELFSEEYCLFLNTKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRG
                     TVGNVAYYDELIKYKHLRINSGMVILRPKTPNLKFACEIAIKVWRNSLKFSII"
     gene            2474..5824
                     /locus_tag="VYN80_001624"
     CDS             2474..5824
                     /locus_tag="VYN80_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1541506.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQVLLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(6330..6800)
                     /locus_tag="VYN80_001625"
     CDS             complement(6330..6800)
                     /locus_tag="VYN80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1541507.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(6817..9090)
                     /locus_tag="VYN80_001626"
     CDS             complement(6817..9090)
                     /locus_tag="VYN80_001626"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1541508.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2956 a         1506 c         1925 g         2800 t
ORIGIN      
        1 aataataatt atagtcgagt gatatcagga agtgctcagc ctcagttacc aattacaaaa
       61 ttaaaaaaaa tacttctccc cctcccccca ctagccctcc aaaatgagtt cgcagacttt
      121 gtagtccagg tcgacaaatc acaatcacaa ttggcaatcc aaaaatctct ggaagaactt
      181 gaaactttga agaaatctct gatgcaggag tagtttggct gatattctgc cattgtaatt
      241 acggtaatga tttgttataa tacttcaaag gaggaaatca gatggtagta aaaacaagaa
      301 aacaaggaaa ttcaatcacc attacgattc caagtgaatt taatattcca agtggtgtta
      361 aatacgaagc gaaattgtta ccaagtggtg agattatctt tactcctgaa gaattggggc
      421 agcaggtttc ttatgtatct gatgatgcct ttgacttaaa tttagataaa atatttgacg
      481 aatacgacga tgttttcaaa gctttggtgg aaaaatgaca atctatctga cagaaaagca
      541 aattgaaaaa ataaatgctt tagcaattca acggtattct ccaaatgaga aaattcaaac
      601 agttagttct tctgccttaa atatgattgt gaacttacca gaacaatttg tctttgggaa
      661 gtctctttat ccaacaattt ttgataaagc aacgatacta tttgtccaat tgataaagaa
      721 gcatgttttt gctaatgcta ataaaagaac tgctttcttc gttttggtca aatttttaca
      781 attaaacggc tatcgttttt ctgtaacggt agaagaagca gtaaaaatgt gtgtaaccat
      841 cgcagtagaa gctttaactg atgaaaaaat gacaagctac tccaaatggg tttctgaaca
      901 ttctgttaga gaaaaggtca aaaagtaacc tagtatgctg gatttgaatg agcacaagaa
      961 aataaatgaa cagacaatat tagaattctg taatgcagaa actgatattg tctcttttta
     1021 ttgatgaata agaaagtgag aaattatgga atcaaaagtt acaattatca tgcaagaaat
     1081 gttacctctt ttaaataatg aacaattact agcgttgaga gagagtttag aacatcatct
     1141 agtagacgga aaaaagcagc agaagtattc gaataataac ctgttgcaac tatttattac
     1201 cgccaagcag gtagagggct gtagctcaaa aacaattcgt tattaccaga ggacgattga
     1261 aaacttgttt aatgctatta aagagtctgt gacacaactc acaacagatg atttaaggag
     1321 ttatttagca aattaccagt ctgaaaagga ttgtagtaag gcaaatttag acaatattag
     1381 gcgtatattg tcttcttttt ttgcttggct tgagcaagag gaatatatca ttaaaaatcc
     1441 cattcgacgg ataaagaaaa ttaagactga gcaaaatgtg aaggaaactt atactgatga
     1501 acatttggaa attatgcgtg ataactgtga aaatttgaga gatttggcaa taatagacct
     1561 actagcatcg acaggtatgc gtgtagggga gcttgtacag ttgaatcgtt cagatattga
     1621 ttttgaaaac agagagtgtg ttgtctttgg taaaggaaag aaggagagac cagtatattt
     1681 tgacgctcgg acgaaaattc atttaagaaa ttatcttaac gacagaaaag atagtcaccc
     1741 tgctcttttt gtaacgctag ttggaaaagc ccagagactt ggaattgctg gtgtagagat
     1801 tcgcttaaga aagttaggag acaaactcgg catacaaaag gttcacccac ataagttcag
     1861 aagaacttta gcgactaagg caattgataa aggtatgcct atcgaacaag tccaaaaact
     1921 gctaggtcat agcaagattg acacaaccct ggcctatgcc atggtcaatc aaaataatgt
     1981 caagcattca caccaaaaat tcatctctta aaagcaaatc ccgatttaac gagatgtttg
     2041 gggaaaataa aatatttgaa agcattgata acttatttga tattatagat ggtgataggg
     2101 gcaaaaatta tcctaaatca gatgagttgt ttagtgagga gtactgttta tttttaaata
     2161 caaagaatgt tactaaaaac ggattttcat tcgatacaaa gcaatttatc actaaaacaa
     2221 aggataaatt acttcgaaaa ggcaaacttg agcgttatga tatagtcttg acaacaagag
     2281 gtactgttgg aaatgtagcg tactacgatg aattaataaa atataaacat ttacgtataa
     2341 attcaggtat ggtaatatta cgtcccaaga caccaaatct aaaatttgct tgtgagatag
     2401 ctataaaagt gtggagaaat agcttgaaat ttagtataat atagctaaac tatttgttta
     2461 aagtgagaaa aaaatgggaa attttagctt tcttttaaaa aatgacgaat atgaatcttt
     2521 ttcaaaacct tgcattgaag ctgagaatat gattgctaca tcaactgtgg ctactgcctt
     2581 tatggcgcgt cgtgctttag agcaggctgt ccattggata tatagtcacg attcatattt
     2641 agaagctccc tatcgtgcta ctctatcttc tttagtatgg gatgatgatt ttagggatat
     2701 cgtagattct gaactccaca agcagatagt tctgttgatt cggtggggaa accatgctgc
     2761 tcatggtggt gaaattaagg aacgagaagc gattttagct ttgcatcatt tgtatcagtt
     2821 tgttaatttt atcgattatt gttacagcaa tgagtttgtg gagcgttatt ttgatgagaa
     2881 gtgcttacca ctttcagcaa acatcaaata ccgagaaact ccacaatcta tgataaagtt
     2941 acaagacagt ttaccagaac tgcctgattt tcatgaacag atggctgctc agtccgtaga
     3001 agttcaagag acttatactg aaaaacgtga gactgcagcg caacggcaag atgtgccttt
     3061 ccatattgat caattatctg aggcagagac aagaaagctc tttattgata tcgatctccg
     3121 tttagcagga tggatatttg aagaaaactg tcgtgttgag atagccgttg atggtctcaa
     3181 gcacggttca ggaattggtt actgtgacta tgtactttat ggtaaaaatg ggaaaatttt
     3241 agcgattgta gaggctaaaa aagcctctgt caatccagaa gtaggggaag tacaggtcaa
     3301 agaatatgct gaagctcttg agaaacatat cggctatcag ccaatttgct ttattacaaa
     3361 tggcttgaag cactatatac ttgatggtcc gaaccgccgc cagattgcag gcttttactc
     3421 tcaagaagaa ttgcaattag tgatggatag acgtcatctt caaaaaccac ttgaggatat
     3481 ttctagtaaa attagggacg atatttccgg gcgtcactac caaaaacatg ccattgcaag
     3541 cgtttgtgaa gctttctcta atcatcgtag acaggtactt ttggttatgg caactggggc
     3601 tgggaaaact cgtacagcag tttctctagt tgatatctta tcacgtcata actgggtaaa
     3661 aaacgttctc ttcttagccg atagaacttc cttggttaag caagcctatg attcgtttag
     3721 aaaattactc ccagatcttt ccgtttgtaa cttcttagaa gataaagaag gagctcaatc
     3781 aagtcgcatg gtcttttcaa cttatccgac catgattgga gcgattagtg gtcaagaaga
     3841 agtaaatcaa cgccctttca ctgttgggca ttttgacctt atcataattg acgaatctca
     3901 ccgttctatt tatcagaaat acaagtccat ttttgattat tttgatgcaa gaattgtagg
     3961 cttaacagct actccgcgtc aagatttaga taaaaacacc tatggattct ttaatttgga
     4021 gaatggggtt ccaacatatg catatgattt ggaagaggct gttaaagacg gatatttagt
     4081 agcctatcat tctatcgaaa ccaaactgaa actacctacg gatggtctac attatgatga
     4141 tttgtccgaa gaagaaaagg aacattttga tagcaaattt gaagacgata gctgtgaaaa
     4201 agatattgat gggagtgtat ttaattcctt tgttttcaat aaaagtacag tagaaattgt
     4261 tttaaatgaa ctcatgacaa gaggaattca gacagcctcg ggtgatgaaa ttggtaaaac
     4321 tattattttt gctaaaaatc atgatcatgc ggaatatatc agaggtattt ttaacaaccg
     4381 ctatcctgaa aaagggagcg actatgctca ggtgattgat tatagtatta agcattatca
     4441 gaccttgatt gatgatttta aaattaagga gaagtatcct caaattgcga tttctgtcga
     4501 tatgttagat acaggtattg atgtaccaga ggttgttaat ttagtcttct tcaagaaagt
     4561 acgctctaaa actaagtttt ggcagatgat tggtcgagga acccgtctat gtaaagattt
     4621 atttggacct gagcaggata aggaaaactt cttggtattt gattatgggg acaattttga
     4681 ttattttcgt gcagatccaa gagatggaga gggtcgtcac attgtttcgt tgactcagcg
     4741 tttatttaat atcaaagtgg acttgattcg agaacttcag ggactccaat accaagaaga
     4801 tcagtttgcg agagcatacc gtcagcagct tgtctcggaa cttcaaggtc gtatagagag
     4861 cttaaatgag ttggacttca gggttcgtat ggttttagat acagtttata gctataggaa
     4921 attggaaagt tggcagaatc taactgctgt tacaagtgaa accattcaaa aaaatctctc
     4981 tctgctttta tttgatgaag ataaagaaga tgagatggcg aggagatttg atttgtggtt
     5041 gcttcatatt cagttggggc aactgacagc taaatcttcc actgttcata tttcccaagt
     5101 gatgaagacg gctagagctc tttctgctat tggcaatatc ccgcaggttt ttgagcaggc
     5161 tgaaattatc aggaaagtac aggagcctga attttggaaa gaagttaact tgtctgattt
     5221 ggaaaaaatt cgtcttgcta ttcgagattt attacagttt ttggataaaa cagaccgtaa
     5281 accctactat gttaactttg aagatcgtat actctccact gttcacgaga ccacagcatt
     5341 tttgcaggtc aacgatcttc ggtcttacaa tgaaaaagtt gagcattatt tgaaaactca
     5401 tctggatgag gagtccattt ctaagctata ccataataaa aagttgacat ctgatgatat
     5461 gcttgcactt gaaaaattgc tttgggaaaa attaggtagt aaagcagact accaaagtca
     5521 ttatgaaaat aaggcaattc cgagattggt tcgtgagatt attggcttag atagagagtc
     5581 tgccaatcgt attttttcta aatttttgtc ggatgagaat cttaatgcca ggcagatttc
     5641 atttgtaaaa ttgattgtag actacattgt agaaaatggt tttttagaga cgaaagtgtt
     5701 aacgcaagag ccgtttaaat cttatggttc tgttcaacta ctcttccaac accaactacc
     5761 agtacttcgt aatattgttc aaatcattga acttatcaat aatcgagctg gagaagcggc
     5821 ttaaattcta aagtgattgc catgctgaga ctcatttaaa attaaaaaga gtagaaattt
     5881 atgctatata tgagaagttt tattaggaag aatgtcatcg ttttcctaga atacagtatc
     5941 agttgttaag tggttgataa atttcaaagt agatacttgt accacgatgt ttgttgatcg
     6001 agttattaac aaaagagcta ctttgatttt aaagaaatag aaaacaaaaa gccgagcaag
     6061 aattcaattg caggagaaaa tgaaataata ctcaatgaaa atcaaagagc aaactaggaa
     6121 actagctgca ggctgctcaa aacactgttt tgaggttgta gatggaagct gacgcggatt
     6181 gaagagattt tcgaagagta taaatcttcc taggataaag caaaacgcat agtatcaagg
     6241 gttttcaaca cttgatacta tgcgttttct gatgttaaag actttctacc aggtttttta
     6301 aaagcataat tgttagttgt agtcatttat tattcttcaa agaaaaatgg tggggcgaat
     6361 tttttcagtt cttcaaagca cttttgagca gtatctgcat cttcacagat gataagacag
     6421 acatcattac cacaaagggt agcgatagcg tcagggaagc tcaaagtatc aatgatagaa
     6481 ccaaaggatt gagccagtcc aggaagggtt tttagtagga cttggtgttg aactgggcgc
     6541 atccagacaa gggcgtcttc catgtagagt tcgagacgtt tttcccattt tgagatggaa
     6601 ccattgttaa gaacataata agcgctatct tcttcgcgga cttttgatag gttcatattt
     6661 ttgatgtcgc gtgagagggt tgcctgggtt acttgaatgt cgttctcagc aagaagggct
     6721 tgcaactcag cctgtgtatg aatcttgttt tttgtgataa gagcgcgtat aagttggtgg
     6781 cggtgttctg atttattcat aataatgtaa ctccttttag caaggtaagg taagcatgga
     6841 ctgagcgagg tcgacagtca agtggtagtc tgtattgtca cggatggtga tttcaaagtc
     6901 agtagtatag aggactaaac ggagagtgtc tccttctttt agcttgtaaa tagttggctg
     6961 cagttcaaat tgaacgtcca tccattcatc tgcagtaata tcctctacta acagtaaatc
     7021 atttctattt tgtaaattaa ggtaaccttt tgtcacgact cgttgtgcct ctggtctaaa
     7081 tggcaattca cagagatttt ccaacatgtg atagcgaccg ttgtcaatgg ttctagcact
     7141 taaaatagct ggataaggtt gtaggtattt cttttgccca aattctagca gttgggcaga
     7201 taagagcccc ttgtttgtac tggatttgat acgaagattg agctgagcgc gaccgtttag
     7261 gtggagatct ttagtcacag gaaggttaat agtaatctga ttggctttcc cttgatagag
     7321 ctctgtattg aaggtttggt atgtcttacc atagcgctca aaatccttat ctgggtactg
     7381 gttttgaata gcttgctctt cttgaccaag tgagaaggtt tcacagtttt cttgcccacc
     7441 gaagttatca agtgataacc aagtctgtgg agctgtattg tcctgccaga taacagtagg
     7501 aagttgaaag tctgtttcct gtcctagtaa tttcttggtc aataaggcat ttatggactc
     7561 acggaagtca attgattgcc aattgttcat gtaaacatgg gcaccattat ggaaaaagag
     7621 atgcttgtgt atatgagtag gaagagcatg gaacatctgg taaacatgaa gtggtttgac
     7681 attccaatcc tgagaaccat gagtaaagac aacctctgcc tttactttat gggcattgag
     7741 cagataattg cggtcatgcc aaaactgatt gtagtcccca gtttttcggt ctagctgagc
     7801 tttcactttt tctaagtcag cttggtgagc ttcattgcca cggatatagt cgccagctaa
     7861 gagattacga gaataggtta actcagcaag ggagtcaaag tcctcacctg gataaccacc
     7921 tgggctagtc accagaccgt tttcacggta gtagttgtac catgatgaaa ttcctgcctc
     7981 ggcaatgata acttctaaac catcgactcc tgtagtcgca agaccattgg acatggtacc
     8041 tagataggaa agtcctgttg tagcaacttt tccgtttgac caatcagcct tgacttgacg
     8101 ctggcgcgtg tgatcagtaa aggcacggca acggccgtta agccaatcga tgacattttt
     8161 ataagcctcg atttgctggt agtctccatt agtcatgaaa cctgtcgagt ctttggtacc
     8221 aacacctgag acatagagat tggcaaagcc tctcggaagg aagtagtcgt ttagtgtata
     8281 gctagagttg atgtgagtta gcttttcctc agcctctgct ataagctcag ctttaccttg
     8341 gggttggacg agatttagtt gaggtttctc tagctcaatc ttgtgaggaa gtttaacctc
     8401 aagctcgccc tccatcttgt agagagcctt gtcactagcc ttgtcattgg ttccctgatg
     8461 ataagggctg gctgtcatga tggcagggat ttttccatca aaacgagggc gaataatgct
     8521 aacctttact aggtctgata gccctttttg gtcagtatcg acacgagact caacgtaaac
     8581 gacttcacga atgacatcct ggttagaaaa agtagccaaa ctcttgccgt taaagtagtg
     8641 gtagtcatta tcctccggaa taagaccatc actaacaagt tggtcgataa gagtatttcc
     8701 ttttttggtg cgagtattga gtaactgata gagattttca atcaagtcac catatataat
     8761 gggaaatcca gtttctttac gaaaaacgtc actatcttcg aagtcaacca aataagcaaa
     8821 gcctaaaagt tgaaaagcaa cagtataaaa aatatctgct gtcagttcat cttctgattg
     8881 aaaaaatgtc agcaggtctg tttttttatc agctgctagg atagaaagtg ggtagttggt
     8941 gtcttgataa gtgaaaaaga aacgacgtaa aaaggtttca agtgagtctt tgtgattggc
     9001 tgtattttgt aaatcaaagc cacatttttt tagttcagat aagacatttt cttttggaaa
     9061 attgatataa ctatattgat taaaacgcat agaacctcca tatagaatga cagttaaggt
     9121 tattatatca aaaaaaaagc agaaagggaa ttgttaactt caaaaggaaa taatccaata
     9181 aaaatga
//
LOCUS       DAVLWA010000074         9048 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLWA010000074 DAVLWA010000000
VERSION     DAVLWA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.074"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..614
                     /locus_tag="VYN80_001627"
     CDS             <1..614
                     /locus_tag="VYN80_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1541509.1"
                     /translation="DRNYDDLEYIYRIEIERSTFLTLEDRTYLEWIQAIIDFYQYDSK
                     CEAISSLENILLKVSSNTLIYLKALNTLSNFYSLVGREQEYEANYSHLIELYQTKNLD
                     HQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEALETIELCKQRQTSYQLAPLLILVGNA
                     GAKFLDREQVKNYYIEARELCKIYNNPLMLMKIENYLKELDTV"
     gene            997..1617
                     /locus_tag="VYN80_001628"
     CDS             997..1617
                     /locus_tag="VYN80_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541510.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1703..2146
                     /gene="tsaE"
                     /locus_tag="VYN80_001629"
     CDS             1703..2146
                     /gene="tsaE"
                     /locus_tag="VYN80_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1541511.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVVILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2136..2654
                     /locus_tag="VYN80_001630"
     CDS             2136..2654
                     /locus_tag="VYN80_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541512.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEATEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2662..3678
                     /locus_tag="VYN80_001631"
     CDS             2662..3678
                     /locus_tag="VYN80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1541513.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            3762..5018
                     /locus_tag="VYN80_001632"
     CDS             3762..5018
                     /locus_tag="VYN80_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1541514.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5073..6239
                     /gene="recA"
                     /locus_tag="VYN80_001633"
     CDS             5073..6239
                     /gene="recA"
                     /locus_tag="VYN80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1541515.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6546..7916
                     /locus_tag="VYN80_001634"
     CDS             6546..7916
                     /locus_tag="VYN80_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1541516.1"
                     /translation="MNKKRTVDLIHGPILLSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8292..>9048
                     /gene="lytA"
                     /locus_tag="VYN80_001635"
     CDS             8292..>9048
                     /gene="lytA"
                     /locus_tag="VYN80_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1541517.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFN"
BASE COUNT         2731 a         1546 c         2036 g         2735 t
ORIGIN      
        1 acgatagaaa ttatgacgat ttggaatata tttatagaat agagattgaa cgaagtactt
       61 ttctaacact agaagaccga acttaccttg aatggattca agctattatt gacttctatc
      121 aatatgacag taagtgtgag gcaatttctt cattggaaaa tatattatta aaagtctctt
      181 caaatactct gatttattta aaggcgttga atactctatc taatttctat tccttagtgg
      241 gtcgtgaaca agaatatgag gcaaactact ctcatttaat agagttgtat cagacaaaaa
      301 atttggacca tcaagagttt ttatttggct acatcagagt tcgttacaac tacgctcact
      361 acctagtatc aaaggaaaaa tataatgaag ccatccaaga agctcttgaa acgattgaac
      421 tctgtaaaca aagacagaca agctaccaat tggctcctct acttattctt gtaggaaatg
      481 ctggagccaa atttctagac agagaacaag tcaaaaatta ttatatagaa gcaagagagt
      541 tatgtaagat ttataacaat cctttaatgt tgatgaagat agaaaattat ttgaaggaac
      601 tagatactgt ttagttaatc ttgacattat agtttttctg aaacgttaaa taacctgtaa
      661 ggctgatagt tagattaata ctatcagcct tataattatg tgactctgag tccgtctcgc
      721 tccgtgaggt gcgagacaaa acaaccaccc gtttgacgga ttttcatgat ttcaaattta
      781 tattcttcga aaatctcttc aaatcacgtc agctctatct gcaatctcaa aacagtgttt
      841 tgagcaacct gtggctagct acctagtttg atctttgatt ttcattgagt attagtctct
      901 agtgtttgtt tagcgtctta aagagacatt tgagaagatt agaaacagtt ctctcttttc
      961 tcctagtata atgattggta ggaagggaga gagaagatga aatcaatgag aatcttattt
     1021 ttgttagctt taattcaaat cagtttgagt agctgtttct tatggaagga atgcatcttg
     1081 tcctttaaac aaagtacagc ttttttcatc ggaagcatgg ttttcgtttc aggaatctgt
     1141 gctggagtaa attatcttta tacccgtaag caagaagtcc atagtgtcct agccagtaag
     1201 aagtcggtga agctttttta cagtatgtta ctcttaatta atttgttagg agctgttctt
     1261 gttttgtcag ataacttgtt catcaaaaat acgctgcagc aagaattagt tgacttttta
     1321 ttgccatcct tctttttcct atttgggcta gatttgctga tttttttacc cttgaaaaaa
     1381 tacgtgcgcg attttcttgc tatgctagac agaaaaaaga cagtgttggt gactatttta
     1441 gcaacacttc ttttcttaag aaatccaatg accattgtct cacttctgat ttatattgga
     1501 ctgggcttgt tttttgcagc ctatcttgtc ccaaattcgg ttaagaagga agtttccttt
     1561 tatggtcata ttttccgaga tcttgtattg gtcattgtta cgctcatttt cttttagaga
     1621 aagtctgttt tttatggatg ttatgatgat aagtttagta ggaaaataga cacaaaagaa
     1681 aagtttttgg tataataaga gtatgtacac aaaaaatgaa gaagagttgc aagccttagg
     1741 ggagcgtttg ggccatctat tagcaaagaa tgatgttgta atcttaactg gagaactggg
     1801 tgcaggtaaa acgaccttta ctaaaggact tgcaaaagga ttacagattt ctcaaatgat
     1861 taaaagtccc acctatacta tcgtgagaga gtatgaaggt cgacttccac tttatcacct
     1921 agatgtttat cgtattgaag gagatgctga ttctatcgac ttggatgagt ttatctttgg
     1981 tggcggcgtg actgttattg agtggggaaa tctcttagga gatgccttgc cagatgctta
     2041 tttagaattg gaaattctaa aagaagcaga tggacgccgt ttaaattttc aggcaaaggg
     2101 tttgcgtgct gagaaattgt tagaggagct tcaatatgga gtatgaattg ctcattaggg
     2161 aagcagagcc caaagatgca gctgaattag tggccttttt aaatcgtgtg agtttggaga
     2221 cagactttac cagcctagac ggagatggta ttctcttgac tagtgaggag atggaaatat
     2281 tcctcaacaa gcaagctagt tcggacaatc agataacctt acttgcattc ttaaatggca
     2341 aaattgctgg tattgtaaat attacagctg atcagcgtaa gagagtccgt catattggag
     2401 atctcttcat tgtgattgga aaaagatatt ggaataatgg cttgggaagt ttgttgctag
     2461 aagaagcgac agagtgggca caagcaagtg gcattctgcg tcgtctccaa ctgactgtcc
     2521 aaactcgtaa tcaagcagca gtccatcttt atcaaaagca tggctttgtc attgaaggta
     2581 gccaagagcg tggggcatat atagaagaag ggaaatttat cgatgtttac ttgatgggta
     2641 aactgatagg ttagtagaaa tatggttaaa aaaattattg gaatggtgct agctttactt
     2701 tctgtaactg tagtaggagt aggtgttttt gcttatacta tttatcaaca agggacagaa
     2761 accttagcta aaacctataa aaaaatcggc gaagaaacca aggttattga agcgactgaa
     2821 cctctaacca ttctgttaat gggagtggac accggaaatg ctgaacgaac tgaaacttgg
     2881 gtcggtagaa gtgatagcat gatcttgatg acagtgaatc ctaaaacgaa aaaaacaaca
     2941 atgatgagtt tagagcggga tattctgacg cgcattgaat cagggaatgg tcaggctcat
     3001 gaagcgaaac tgaactcagc atatgcagat ggtggagcag agcttgctat agaaaccatt
     3061 caaaaaatga tgaatatcca tattgatcgc tatgtgatgg tcaatatgag agggttgcaa
     3121 aaactagtgg atgcagtagg aggtattaca gtcaataata tcctaggttt cccaatttct
     3181 atcagtgacc aagaagaatt taatactatt tctatcggtg ttggggagca acatattggg
     3241 ggagaagaag ccctagtcta tgcacgaatg cgttaccaag atcctgaggg ggattatggt
     3301 cgtcaaaaac gtcaacgtga agttattcaa aaagtcatgg aaaaagctct cagtttaaat
     3361 agcgttggtc attatcaaga gattctaaaa gctttgagtg acaatatgca gaccaatatt
     3421 gatttgtctg caaaaagtat ccctaacttg ctaggctata aagattcatt taaaaccatt
     3481 gaaactcagc agttgcaggg tgaaggagag atacttcaag gtgtttctta ccagattgtt
     3541 tcgagagcac atatgttgga aatgcaaaat atactccgac gttctttggg acaagaagaa
     3601 gttactcagc ttgaaaccaa tgcggtttta tttgaagatt tatttggcag agcacctgtt
     3661 ggtgatgaag ataattaact tttgatacaa ataaaaaatc aatcgtagga aaattcctgc
     3721 gattttttca aaaaaatacg aatagatagg taggaggaaa catgaaagca gaaatcattg
     3781 ctgttggaac agagattttg acaggacaga ttgtcaacac caatgcccag tttttgtcgg
     3841 aaaaactagc tgagattggg gtagatgtat attttcagac ggctgtagga gacaatgaag
     3901 ttcgtctctt gtctttgctt gagattgcca gtcaacgtag cagtctggtg attttgacag
     3961 gcggtttggg gccaactgag gacgacctaa ccaaacaaac cctagctaaa tttttaggga
     4021 aagcattagt ctttgatcct caggctcagg agaagttgga tatctttttt accctgcgac
     4081 cagactatgc ccgaacacca aataacgaaa gacaagctca aattgtagaa ggagcgattc
     4141 cactgccaaa cgaaacagga ctggctgttg gaggaaaatt agaagtagac ggagtgacct
     4201 atgtcgtcct tccaggtccg ccaagtgaat tgaaacccat ggtcttaaac caacttctac
     4261 ccaagttgat gacagggagc aagctgtatt cccgagttct tcgtttcttt gggattggcg
     4321 agagccagtt ggttacgatt ttggctgatt taattgataa tcagatcgat cctaccttgg
     4381 ccccttatgc caagacagga gaagtcactc tacgtctgtc aacaaaggct agcagtcaag
     4441 aagaggcgaa tcaagcgctg gatatcttgg aaaatcaaat cttggactgc cagactttcg
     4501 aaggaatttc tttacgagac ttttgctatg gttatgggga agaaactagt ttagccagca
     4561 ttgtggtaga agaactgaaa aggcaaggga aaaccatcgc ggctgcagag agtttgacgg
     4621 caggtctttt ccaagctacc gtggcgaatt tttctggagt ttcaagtata tttgagggtg
     4681 gttttgtgac ctatagcttg gaggaaaaat caaggatgtt ggatattcct gccaagaatt
     4741 tggaagaaca tggtgtggtg tctgaattta cagctcagaa gatggctgag caggcacgaa
     4801 gcaagaccca gtctgatttt ggaattagtt tgactggagt ggcaggacca gatagcctag
     4861 aaggacaccc agttgggaca gtcttcatag gcttggcgca agatcaagga actgaggtta
     4921 tcaaggttaa tattggaggc agaagccgag cagatgtacg tcacattgcg gttatgcatg
     4981 cctttaacct agttcgcaag gctttattaa gtgactaact tttgatataa tagtagatag
     5041 gtctgaggat cattagaatg taggagaata gaatggcgaa aaaaccaaaa aaattagaag
     5101 aaatttcaaa aaaatttggg gcagaacgtg aaaaggcctt gaatgacgct cttaaattga
     5161 ttgagaaaga ctttggtaaa ggatcaatca tgcgtttggg tgaacgtgcg gagcaaaagg
     5221 tgcaagtgat gagctcaggt tctttagctc ttgacattgc ccttggctca ggtggttatc
     5281 ctaagggacg tatcatcgaa atctatggcc cagagtcatc tggtaagaca acggttgccc
     5341 ttcatgcagt tgcacaagcg caaaaagaag gtgggattgc tgcctttatc gatgcggaac
     5401 atgcccttga tccagcttat gctgcggccc ttggtgtcaa tattgacgaa ttgctcttgt
     5461 ctcaaccaga ctcaggagag caaggtcttg aaattgcggg aaaattgatt gactcaggtg
     5521 cagttgatct tgtcgtagtc gactcagttg ctgcccttgt tcctcgtgcg gaaattgatg
     5581 gagatatcgg agatagccat gttggtttgc aggctcgtat gatgagccag gccatgcgta
     5641 aacttggcgc ctctatcaat aaaaccaaaa caattgccat ttttatcaac caattgcgtg
     5701 aaaaagttgg agtgatgttt ggaaatccag aaacaacacc gggcggacgt gctttgaaat
     5761 tctatgcttc agtccgcttg gatgttcgtg gtaatacaca aattaaggga actggtgatc
     5821 aaaaagaaac caatgtcggt aaagaaacta agattaaggt tgtaaaaaat aaggtagctc
     5881 caccgtttaa ggaagccgta gttgaaatta tgtacggaga aggaatttct aagactggtg
     5941 agcttttgaa gattgcaagc gatttggata ttatcaaaaa agcaggggct tggtattctt
     6001 acaaagatga aaaaattggg caaggttctg agaatgctaa gaaatacttg gcagagcacc
     6061 cagaaatctt tgatgaaatt gataagcaag tccgttctaa atttggcttg attgatggag
     6121 aagaagtttc agaacaagat actgaaaaca aaaaagatga gccaaagaaa gaagaagcag
     6181 tgaatgaaga agttacgctt gacttaggcg atgaacttga aatcgaaatt gaagaataag
     6241 ctgttaaagc agtggagaaa tccgctactt tttcgatttt tgattcaagt ttttagatta
     6301 tatatagtag cttgaaataa gatatgaaca actttattag gaaagtcaaa ttaatttcta
     6361 gaaatgtttt agcagctaca gcgtactatt ccaaactcaa ccaactataa tagatcgaaa
     6421 ctagaatagt acacatctac ttctaaaaca ttgttaaaaa tcgatttgac tgtccttatt
     6481 tcattccgct atatataagt ttgctgtttc ttgtcgctcc tctggaaagc tgatataata
     6541 gctttatgaa taaaaaacga acagtggacc tgatacatgg tccgattctt ctctcgctct
     6601 taagcttcgc cttcccaatc ttgctatcaa atatttttca acagctctat aacactgctg
     6661 atgtcttgat tgttggacga tttcttggtc aagaatcctt ggctgcagta ggagcgacga
     6721 cagcgatttt tgacctgatt gtaggtttta cacttggtgt tggcaatggc atggggattg
     6781 tcattgctcg ttattatggg gctcggaatt tcactaaaat caaggaagca gtagcagcca
     6841 cctggatttt aggtgctctt ttgagcattc tagttatgtt gctgggcttt cttggcttgt
     6901 atcctctctt gcaatactta gatactcctg cagaaattct tcctcaatct tatcaatata
     6961 tttctatgat tgtgacctgt gtaggtgtca gctttgctta taatcttttt gcaggcttgt
     7021 tgcggtctat tggtgacagt ctagcagccc tgggatttct gattttctct gccttggtta
     7081 atgtggttct ggatctctat tttattacgc aattgcatct gggagttcaa tccgcaggac
     7141 ttgctaccat tatttcgcaa ggtttatcag cggttctctg cttttattat attcgtaaaa
     7201 gtgtgccaga actcttgcca cagtttaaac atttcaaatg ggacaaaagc ttgtacgcgg
     7261 atctcttgga gcagggtttg gctatgggct tgatgagttc aattgtatct atcggcagtg
     7321 tgattttaca gtcttctgtt aatacatttg gtgcagtgat tattagtgcc cagacggcag
     7381 ctcgacgcat tatgaccttt gcccttcttc ctatgaccgc tatttctgca tcaatgacga
     7441 cctttgcttc tcagaatcta ggagctaagc gacctgaccg cattgttcaa ggtcttcgaa
     7501 tcggcagtcg tttaagtata tcctgggcag tttttgtttg tattttcctc ttttttgcca
     7561 gtccagcctt ggtttccttc ttggctagtt cgacagatgg ttacttgata gaaaatggaa
     7621 gtctctatct gcaaatcagt tcagcctttt atcccatttt gagcctcttg ttgatttatc
     7681 gcaattgctt gcagggcttg gggcagaaga tccttcctct agtttctagc tttattgaac
     7741 taatcggaaa aatcgttttt gtggttttga ttattccttg ggcaggatat aagggtgtta
     7801 tcctttgtga acctcttatc tgggttgcca tgacagttca actgtacttc tcactattcc
     7861 gtcatccctt gataaaagaa ggaaaggcaa ttttggcaac caaagtgcaa tcctagttgg
     7921 atttactgaa taaaatccat ttcctctagt gaaaatcgaa aaaacttgtg ttctcttctt
     7981 tagtttggtg ttgaaaatag tttaacagac ttttgacttc ttttatatga tataataaag
     8041 tatagtattt atgaaaagga catatagaga ctgtaaaaat atacttttga aaagcttttt
     8101 agtctggggt gttattgtag atagaatgca gaccttgtca gtcctattta cagtgtcaaa
     8161 atagtgcgtt ttgaagttct atctacaagc ctaatcgtga ctaagattgt cttctttgta
     8221 aggtagaaat aaaggagttt ctggttctgg attgtaaaaa atgagttgtt ttaattgata
     8281 aggagtagaa tatggaaatt aatgtgagta aattaagaac agatttgcct caagtcggcg
     8341 tgcaaccata taggcaagta cacgcacact caactgggaa tccgcattca accgtacaga
     8401 atgaagcgga ttatcactgg cggaaagacc cagaattagg ttttttctcg cacattgttg
     8461 ggaacggttg catcatgcag gtaggacctg ttgataatgg tgcctgggac gttgggggcg
     8521 gttggaatgc tgagacctat gcagcggttg aactgattga aagccattca accaaagaag
     8581 agttcatgac ggactaccgc ctttatatcg aactcttacg caatctagca gatgaagcag
     8641 gtttgccgaa aacgcttgat acagggagtt tagctggaat taaaacgcac gagtattgca
     8701 cgaataacca accaaacaac cactcagacc acgttgaccc ttatccatat cttgctaaat
     8761 ggggcattag ccgtgagcag tttaagcatg atattgagaa cggcttgacg attgaaacag
     8821 gctggcagaa gaatgacact ggctactggt acgtacattc agacggctct tatccaaaag
     8881 acaagtttga gaaaatcaat ggcacttggt actactttga cagttcaggc tatatgcttg
     8941 cagaccgctg gaggaagcac acagacggca actggtactg gttcgacaac tcaggcgaaa
     9001 tggctacagg ctggaagaaa atcgctgata agtggtacta tttcaacg
//
LOCUS       DAVLWA010000075         8978 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLWA010000075 DAVLWA010000000
VERSION     DAVLWA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.075"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            122..277
                     /locus_tag="VYN80_001636"
                     /pseudo
     CDS             122..277
                     /locus_tag="VYN80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VYN80_001637"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VYN80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1541518.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYIDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERKNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3749..7399
                     /gene="addA"
                     /locus_tag="VYN80_001638"
     CDS             3749..7399
                     /gene="addA"
                     /locus_tag="VYN80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1541519.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALKMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(7361..7573)
                     /locus_tag="VYN80_001639"
     CDS             complement(7361..7573)
                     /locus_tag="VYN80_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541520.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHIYSK
                     AKLTWFEEIFEEYKLYFYNLNLVFTE"
     gene            7704..8882
                     /locus_tag="VYN80_001640"
     CDS             7704..8882
                     /locus_tag="VYN80_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541521.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKIENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQNQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2778 a         1887 c         1896 g         2417 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct acccgtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagctaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgaatt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaagggc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc gccttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgctga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcccgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga cttgtatatt gacctcagtc
     1681 agtctgatat tgatgctttt gaacaatata tccgctatct tggtattaat ggcttgccag
     1741 cctttcagca aaccttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttttg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggaaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagaa
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatctgca aggtctggaa aatccagcta
     2641 tcccaaccag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg gtttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttacagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacgac tggacatatt ctccgacaca atccagccat cgaaaccatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggctaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggt
     3721 ttgaaaaaat aagagaggag ttaaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacagaaga aaactgccga
     3841 acaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg tagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgatctca aacaacactt
     4081 gggttgccag ttggcagacc tacccaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agagctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaagt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggctttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctctggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaattgg ctccctaaat caggagttcg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agccgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttactaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catggagaca cttggaaact agctaaaacc ttccaatctt tcatgagcga
     4861 ttttgtagag gcttatcgtc agagaaaacg acaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagaaaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatcctcaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gcgaaatcaa
     5281 ctatgataac atgcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg aattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggtttga ttcacacagc
     5821 tctagctgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa ggggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag cctgacctat aggcagaatg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaagcca agcaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattggcgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctgaaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagag
     6961 aattgacctc agccagcaac taacccttgc tagcctaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agcactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtataac ttatactctt cgaaaatctc ttcaaaccac gtcagcttcg
     7441 ccttgctgta tatatggtta ctgacttcgt cagttctatc cacaacctca aaacagtgtt
     7501 ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt
     7561 cagttctatc cacaacctca aaacagtgtt ttgagctgac ttcgtcagtt ctatctacaa
     7621 cctcaaaaca gtgttttgag ctgattgcgg ctagctttct agtttgctct ttgattttta
     7681 ttgagtatta gaaaggagac ctcatgacac ttccagttag aaaatcccta cacgatgcag
     7741 ttttacaggc ttcgaaagcg gatacttggg aacaagctac caaggaatgg aatgaagttt
     7801 ccttgatttt taacggtatc ggccgtagca attgtgtctg tggaaatgcc atcaaatacg
     7861 cttatgaact ctttaacggt gtcacaggcc aacgcctctt tccaattggt agcgactgtg
     7921 ttcgtcattt tcatcgattg tcccttgacc agcaattgga agaggaagaa aaactgctca
     7981 gaaagattga aaatctaacc agaaaagctc agaaaaagga aaaaatcaag gtcaataaaa
     8041 gtgactttga cgagcgactt ctaaaatggc tctgggaaaa aggtgttttc aaacccaatc
     8101 gtggcaatca atttgcgcct gagagagact accagctttt cctagaagtc tttcagggag
     8161 gaagttggac caaggcggaa ccaaagaaaa aggctcggat ggaagaagtc cttgaaaagt
     8221 gtatcaaacc ctttttactt gggaaatcgg atgaccaact ctaccttgtc aagctaggca
     8281 aggagaaaat tgactacgaa caagaactcc gtatccaggc agagaaagaa cgcaagaaga
     8341 gggataaaat cgccaagcaa tatgcagaca atctggttct agccatggga cccgcagagc
     8401 gtgcttatca agattacttt ggctttacag agaccctgac tcaagaagaa cgcaagtggg
     8461 aaaaaattct ttttggtaaa aatagagcag aacgggctat caaggccaaa caataccaaa
     8521 aagagctgga aaaagaccaa cgaattgcca gtcaggatcc aattgaaaga aaacagaatc
     8581 agacttggct cctcaattcc tattttcgtg agcttcctga agaaaaagct cgattttcta
     8641 ggctcttatt agaatatcga aaaagtggtg aagtaccctt ttcaactgaa tacctgtcag
     8701 atcatctcat cgactttttc tataaaatga aagcctttga atttgaaatc gcaccagaac
     8761 aagtccgaga ttttctaaaa aaaagcctgc aggaagacca tcgctcatca gcacaaggaa
     8821 gctggataga aggaattctc ctcaactgcc tcaaaccatt tttagaacga ttagtgatat
     8881 gagtaacacc taccgtgtaa atttcatggt aggtgttatt gtacccccaa ataatataaa
     8941 acaaagtatg aaagcgcatc tcaattttaa gacttttt
//
LOCUS       DAVLWA010000076         8769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLWA010000076 DAVLWA010000000
VERSION     DAVLWA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.076"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..535
                     /locus_tag="VYN80_001641"
     CDS             <1..535
                     /locus_tag="VYN80_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1541522.1"
                     /translation="KSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPET
                     FENYNVFVPEANGSGALGEVLSTPLIGEPLIGEPLIGHTDTFLSIGNFKTKFEADACI
                     KFIKTKFARVLLGVLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVADIDLQLDQKYD
                     LSPEEIAFIENHVREMD"
     gene            700..3954
                     /locus_tag="VYN80_001642"
     CDS             700..3954
                     /locus_tag="VYN80_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1541523.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(4340..5326)
                     /locus_tag="VYN80_001643"
     CDS             complement(4340..5326)
                     /locus_tag="VYN80_001643"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1541524.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5520..7988
                     /gene="gyrA"
                     /locus_tag="VYN80_001644"
     CDS             5520..7988
                     /gene="gyrA"
                     /locus_tag="VYN80_001644"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1541525.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLTEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            7988..8731
                     /gene="srtA"
                     /locus_tag="VYN80_001645"
     CDS             7988..8731
                     /gene="srtA"
                     /locus_tag="VYN80_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1541526.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         2833 a         1506 c         1869 g         2561 t
ORIGIN      
        1 gaaatcacta actcagttag attttgtttt caaaaacaac gaagaaaaag accatgttgc
       61 tctacttgga agaattggct ccgaacgtgt ttatcgatat attaataaaa aatatttaga
      121 tttaccggaa acattcgaaa attataatgt ttttgtacca gaagctaatg gaagtggtgc
      181 cttaggtgaa gtcttatcaa cacccctaat cggggaaccc ctaatcgggg aacccctaat
      241 cgggcataca gatacttttt tatctattgg taattttaaa acaaaatttg aagccgatgc
      301 ttgtattaaa tttattaaaa ctaaattcgc tagagtatta ttaggtgttt tgaaagttac
      361 tcagcataat tcacgcaaaa cttggtatta cgtccccctc caagacttta cggtcaattc
      421 ggacattgat tggacacaat cagtggctga tattgacctc cagcttgatc aaaaatatga
      481 cctttcccct gaagaaattg cctttattga gaatcatgta agggagatgg attagaaaag
      541 tatttttatt tgacaaatag tgctcaatga tctaaaatga ctatatagga ttaggtcagg
      601 aagcatacga tgccctgacc ctttttgtac ttatgagatg agaaagtcat ttgttagata
      661 aattgacttc gttagcaaac gttcaaaaaa ggaaaactta tgccagtaga aattaaaacc
      721 actaaagaga ttcatcctaa aatctatgcc tataccacac cgacagtaac cagtaatgaa
      781 ggctggatta agattgggta tacagaacgt gatgtcacac aacgtatcaa ggagcaaacg
      841 catacagctc atatagctac agatgtctta tggactggtg atgcagctta tacagaagag
      901 cctgataagg ggaaaacttt caaggaccat gatttccacc atttcctttc tttccatgat
      961 gtagaacgtc gtcccaagac ggaatggttc tattttaatg gaactcctga aaaatcaaaa
     1021 aatctttttg ataagtttgt tcagcatgat ttgtctggtt atcagcctgg aaaaggacag
     1081 gactatactc tgcgacaaga gcaagaagaa gcagttgcta agacattagc ttatttccaa
     1141 gaacatgctg gaggcaagtt tctctggaat gccaagccac gctttggtaa aaccttgtct
     1201 acctatgacc tagctcgacg gatggaagct gtcaatgtcc taattgtaac aaaccgccct
     1261 gccattgcta actcatggta tgatgatttt gaaacattca tagcaggtca aacgacttac
     1321 aagtttgttt ctgaatcaga tagccttaag agtcgtccaa tcttgtcacg acaagaattt
     1381 cttggtattt tagctgacga tgtaagacaa cttgctttta tcagtctcca agacttgaaa
     1441 ggatctgttt atttaggtgg agagcacgat aaactcaaat gggtaactga tctgcattgg
     1501 gacttgttgg ttattgacga ggctcatgaa ggagttgata ccttcaagac tgaccaagcc
     1561 tttaataaga ttcgacggaa ttttactctg catttgtcag gtacatcatt taaagcattg
     1621 gctaaaggag attttacaga ggaacaaatc tacaactggt cttatgctga tgagcaggct
     1681 gctaagtatt cgtggtctct tgagcaagaa gaggaaaatc cttatgaaag cttgcctcag
     1741 ttgaatctct ttacctatca aatgtctcag atgattggcg aaaagttaga aaaaggcgct
     1801 cagatcgatg gtgaaaatat tgactatgtt tttgacttaa gtgaattttt cgctacagat
     1861 gataaaggga aatttattca tgagcatgat gtcagaaatt ggttagatac tctatcaagc
     1921 aatgaaaaat atccattttc aaccaaagaa ctccgtaatg aactcaagca tactttttgg
     1981 cttttagaac gtgtcgcttc ggccaaagca ttaaaagccc tactagaaga acacccaatc
     2041 tatgaaaact atgagatcgt tctagctgct ggtgacggac gtatgtccga agaagacgat
     2101 aaagtcaaac tcaaatcctt ggacttggtt agaaaagcga tagcagagaa tgacaaaacc
     2161 attaccctat ccgttggtca gctgacgaca ggtgtcacta tccctgaatg gacaggtgta
     2221 ttgatgttat caaatttgaa atcaccagct ctttatatgc aggccgcctt ccgtgctcaa
     2281 aatccttact catggagcga taacaaagga aatcactttc gcaaagaaag agcctatgta
     2341 tttgactttg cgccggaaag aaccttgatt ctctttgatg agtttgccaa caacttatcg
     2401 cttgtaactg cagctggtag aggaacttca gctacacgcg aagaaaatat tagagaatta
     2461 ttaaacttct ttccaattat tgccgaagac cgtgctggta agatggttga aattgatgca
     2521 aaggcagttc taaccactcc tcgccagata aaagctagag aagttcttaa acgaggtttt
     2581 atgtccaatc tcttatttga taatattagt ggtattttcc aagcaagtca aacagtttta
     2641 gatattttaa atgagctgcc agttgaaaag gaagggaagg tacaagatag ttctgattta
     2701 ttagattttt cagatgttac agtcgatgat gagggaaatg cagtagtaga ccatgaaatt
     2761 gtagttaatc agcaaatgcg actttttggt gaaaaagttt atggacttgg tgaatctgtt
     2821 gctgagttag tcacaaaaga tgaggaacga actcaaaaac agctggtcaa tgacttgagt
     2881 aagaccgttt cttcagtgat tgtagaggaa ttgaaagcag attattctct aaaaacaagg
     2941 gaaactgagc aaattaagaa acaaattaca gcaacacttg agaatgaaat tcgaaaaaat
     3001 gatatcgaaa gaaaaatttc tgaagctcat atcaagcaag agttgcaaca gcagctcaaa
     3061 gaagcaaatg ataaagcgca aaaagataag attcaagaag atttagaaaa acgtttagaa
     3121 gaaaataaac tcattcataa agaaaaacta gaacaaacac tcaaaaaaga agtggaaaaa
     3181 atgcctgaga aatttatcga acaggttgag ataaaacgtg tggaacagtt gaaacaatca
     3241 gctcaagatg aaattcgtga ccatttacga gggtttgcaa gaacaattcc aagttttatt
     3301 atggcttacg gtgatcaaac tctaacactt gataattttg atgcctttgt tcctgaacat
     3361 gttttttatg aagtaacagg gattacgatt gatcagttta gatatttgcg agatggtggg
     3421 caggattttg cagggcatct ctttgataaa gcaacatttg acgaagctat tcaagaattt
     3481 cttcgcaaga aaaaggagtt ggcggattat tttaaagatc aaaaagaaga catttttgac
     3541 tatattccac cgcagaagac caaccaaatt ttcactccta aacgagtggt gaaaaggatg
     3601 gtagatgatt tggaaaagga aaatccaggg atttttgatg atccatctaa gacttttatt
     3661 gatttatata tgaagtcagg cctctatatt gcagaacttg tgaagcggtt atataatagc
     3721 aatggcttga aagaggcctt tccaaatcct gaagaacgct taaaacatat tttggaaaag
     3781 caagtttatg gatttgctcc gtctgagatt atctataaca tttccactaa ttttatattt
     3841 ggtaatcttt ctaaagatat cagtaggaag aattttgttt tagcagatac cattccagcg
     3901 gctaaagaag ggagcattca aaagttggtt gattcctatt ttgaaaataa ttaaaaagaa
     3961 ggccaagtca aaattctttg aaatcagaaa aaacgcataa tattgagtgc ttttgtactg
     4021 cccccaaaaa gttagacaga aaaaatctaa cttttggggg gcagttcaga caatccttgg
     4081 tattatgcgt tttattgtgg gaagatgtat aatggattga aataagatat gaacaaatcg
     4141 attaggaaag gaaaattgat ttatagaaat gttttagtag tcgatgcgta ctgttataga
     4201 ttcaacgaac tataaaatct gaagaaagca attttaacca gatcttagaa gcagttgtgt
     4261 actactctaa cttcaataga ctatatcata tagataaaaa acaactccct gatgattcaa
     4321 ggagttgtct atagttaaat tagtttttag aagcttcttg gaattctgga tttttccatg
     4381 cttcgtcaat gatagcttgc aattctttag cagatgcttg cattttttga gtttctgcat
     4441 cgttcaatgg aatgtttact ggacgaacga taccatgtgc accaacaaca gctggttgac
     4501 cgataaagac attctcaact ccgtattgac cttcttggaa tactgaaagt ggaagtactg
     4561 cgttttcatc gtcaaggatt gctttagtga tacgagcaag ggctactgcg ataccgtagt
     4621 atgttgcacc ttttttgttg atgattgtgt aggctgcatc acgaacacct tcgaacaatt
     4681 caatcaattc agcttcttga acattttgag tgtctttaag gaattcttca aggtttacac
     4741 cagcgatgtt agcgtgtgac caaacagcga actcagagtc accgtgttca cccatgatgt
     4801 aggcgtgcac tgaacgagca tccacatcca atttttcagc aagtgcttga cggaaacgag
     4861 ctgagtcaag tgaagtacct gaaccgataa cgcgttcttt agggaaacca gagaatttcc
     4921 aagttgagta agtcaaaacg tcaactgggt tagcagcaac aaggaagata cctttgaaac
     4981 cagattcaac aacttgagtt acgattgatt tgttgatagc aaggttttta cctacaaggt
     5041 caagacgagt ttcacctggt ttttgaggtg cacctgcagt gatcacaaca aggtcagcgt
     5101 ctgcacagtc agagtattga gctgcataga tttttttagg tgaagtgaag gcaagggcgt
     5161 gactaaggtc aagcgcatca ccaacagctt tttcatgcaa ttgtggaatt tcgataattc
     5221 caagctcttg tgcaattcct tggttaacaa gtgcaaaagc gtaagatgaa cctacagcac
     5281 catcaccgac aaggataact tttttgtgtt gtttagttga agtcattgtt ttaaacatct
     5341 ccttaatttt attaggggat tttccctaga caacttcatt ctatcacttt taaaaaactt
     5401 tgtcacgaat atgccttata gttctcgatg taaacgtttt agtggtttag aggctgaaat
     5461 agatgggaat ttatggtata atgttgttac ttactaattg tgaaatgagg catttattaa
     5521 tgcaggataa aaatttagtg aatgtcaatc tgacaaagga gatgaaggca agttttatcg
     5581 actacgccat gagtgttatc gtagcgcgag ctcttcctga tgttcgagat ggcttaaaac
     5641 ctgttcaccg tcgcattctc tacggaatga atgaattggg tgtgacccca gacaaacccc
     5701 ataaaaaatc tgctcgtatt acaggggatg tcatgggtaa atatcaccca cacggggatt
     5761 cctctattta tgaagccatg gtccgtatgg ctcaatggtg gagctaccgt tacatgcttg
     5821 tagatggtca tgggaatttt ggttccatgg atggagatag tgctgccgct caacgttata
     5881 ccgaggcacg tatgagcaag attgctctgg aaatgcttcg tgatatcaac aaaaatacag
     5941 ttgatttcgt tgataactat gatgccaatg aacgggaacc cttggtcttg ccagcgcgtt
     6001 ttccaaacct tttggttaat ggagcaactg gtatcgcggt tgggatggca accaatattc
     6061 cacctcataa tctgggtgaa accattgatg cagtgaagtt ggtcatggat aatcctgaag
     6121 tgactaccaa ggacttgatg gaagtcttgc ctggaccaga ttttccaact ggtgctcttg
     6181 tcatggggaa atcaggtatc cataaggctt atgaaacagg taaaggttcg attgtcctac
     6241 gttctcgtac agagattgaa acgactaaga ctggtcgtga gcgtatcgtt gtaacagaat
     6301 ttccttacat ggtcaataaa accaaggtgc atgagcatat tgttcgcttg gttcaggaaa
     6361 aacgcattga gggtatcaca gcagtacgtg atgagtcaaa ccgtgaaggt gttcgatttg
     6421 ttattgaagt caagcgcgac gcctcagcca atgttattct caataacctc ttcaaaatga
     6481 cccaaatgca aaccaatttt ggtttcaata tgctcgctat ccaaaatggt ataccgaaaa
     6541 ttttgtctct tcgtcagatt ttggatgctt atatcgagca ccaaaaagaa gtggttgttc
     6601 gtcgtacacg ttttgataag gaaaaagcgg aagcgcgcgc tcatatctta gaaggtctct
     6661 tgattgcgct agaccatatc gacgaagtga ttcgtatcat ccgtgctagt gaaacggatg
     6721 cggaagctca agctgagttg atgagcaagt ttaagctttc tgaacgtcaa agtcaagcta
     6781 tccttgatat gcgtcttcgt cgtttgacag gtttggaacg cgataagatt caatctgagt
     6841 atgatgacct cttggctctg attgcggatt tagcagatat tcttgctaag cctgaacgtg
     6901 tttctcaaat tatcaaagac gaattggatg aagttaaacg taaattttct gataaacgcc
     6961 gtacagagtt gatggttgga cagatcttga gtctcgagga tgaggacttg attgaagaat
     7021 cggatgtctt gattaccctt tctaacagag gctacattaa gcgtttggat caggacgagt
     7081 tcactgctca aaaacgtggg ggtcgtggtg tccaaggaac gggagtgaaa gatgatgact
     7141 ttgttcgtga gttagtgtca actagcaccc atgatcatct gctcttcttc acaaacaagg
     7201 gacgtgtcta tcgtcttaaa ggttatgaaa ttcctgagta tggtcggact gccaaagggc
     7261 taccagtagt caatctcttg aaattggatg aagatgaaag tattcagacg gttatcaatg
     7321 ttgagtctga tcgcagtgat gatgcttatc tcttctttac aacccgtcac ggtattgtga
     7381 agagaactag tgttaaggag tttgccaata ttcgtcaaaa tggtctcaaa gcgctgaatt
     7441 taaaggatga agatgagtta atcaatgtct tgttgacaga aggagatatg gatattatca
     7501 ttggtaccaa gtttggttat gcagttcgct ttaatcaatc agccgttcgt ggtatgagcc
     7561 gtatcgccac tggtgtgaaa ggtgttaacc ttcgtgaagg agacacagtt gttggtgcca
     7621 gcttgattac tgatcaagat gaggttctta ttatcacaga aaaaggatat ggtaagcgta
     7681 cagtcgctac tgaataccca acaaaaggtc gtggtggtaa gggaatgcag acagctaaaa
     7741 ttaccgaaaa aaatggcttg ctggccggtc ttatgactgt tcaaggtgat gaggatttga
     7801 tgattatcac tgatacaggt gtcatgattc gaaccaatct tgccaatatt tcacaaacag
     7861 gacgtgcaac tatgggagtt aaagtaatgc gcctggatca agatgctcag atagtgactt
     7921 tcacaacggt tgcggtggca gaaaaagaag aagttgggac agaaaacgaa acagaaggtg
     7981 aagcataatg tctcaaaaaa ataataaaaa gaaaaacaag cgaaaaaatc tgctgacaaa
     8041 tatcctagca ggatttctga tattactgtc actggctttg atttttaata ctcaaattcg
     8101 aaatattttc atagtctgga ataccaataa gtatcaagtt agccaggtat caaaagaaaa
     8161 attagaagaa aatcaggata cagaaggcaa ttttgacttt gattctgtca aagctatctc
     8221 ttcggaagct gttctaactt ctcaatggga tgctcaaaaa ttaccagtta ttgggggaat
     8281 tgcaattcct gaattggaaa tgaatttgcc gatttttaaa ggacttgata atgttaatct
     8341 cttctacgga gctggtacaa tgaaacgcga gcaagtaatg ggagaaggaa attatagtct
     8401 agctagtcac catatctttg gtgttgataa tgctaataaa atgttatttt ctcctttaga
     8461 taatgctaaa aatggcatga agatttatct aaccgataaa aataaagttt atacttatga
     8521 aatacgtgaa gtcaaacgtg tgacaccgga tcgtgttgat gaagttgatg atagagatgg
     8581 ggtcaatgaa atcacattag taacctgtga agaccttgct gctacagaac gtattattgt
     8641 caaaggtgat ttgaaagaaa caaaagatta ttcacaaaca tctgatgaaa tcctaacagc
     8701 tttcaatcaa ccatataaac aattttatta atacaaatca gtgaaatcat ggatttcact
     8761 gatttttta
//
LOCUS       DAVLWA010000077         8545 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLWA010000077 DAVLWA010000000
VERSION     DAVLWA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.077"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            200..1966
                     /locus_tag="VYN80_001646"
     CDS             200..1966
                     /locus_tag="VYN80_001646"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1541527.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASYAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNSDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            2112..3263
                     /locus_tag="VYN80_001647"
     CDS             2112..3263
                     /locus_tag="VYN80_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1541528.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            3408..4292
                     /locus_tag="VYN80_001648"
     CDS             3408..4292
                     /locus_tag="VYN80_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1541529.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            4645..4896
                     /locus_tag="VYN80_001649"
     CDS             4645..4896
                     /locus_tag="VYN80_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1541530.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            4884..5174
                     /locus_tag="VYN80_001650"
     CDS             4884..5174
                     /locus_tag="VYN80_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541531.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(5357..5484)
                     /locus_tag="VYN80_001651"
                     /pseudo
     CDS             complement(5357..5484)
                     /locus_tag="VYN80_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(5579..6910)
                     /locus_tag="VYN80_001652"
     CDS             complement(5579..6910)
                     /locus_tag="VYN80_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1541532.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHKRSEIHIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(6932..8443)
                     /locus_tag="VYN80_001653"
     CDS             complement(6932..8443)
                     /locus_tag="VYN80_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1541533.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
BASE COUNT         2673 a         1610 c         1841 g         2421 t
ORIGIN      
        1 ttaaacaaca tattgacagt ttagaaagca ataatctatc catagactta aaagggctga
       61 agggaaaaaa ggttagaatt tctttgagaa acgcaggaat tcctttaagt ttggcagagg
      121 tagaggttta tacttataag taattttaaa aattatcatc ggtagtatga aagaaacaga
      181 aaaataagag gaaaatagta tgattcaaca tccacgtatt gggattcgtc cgactattga
      241 tggtcgtcgt caaggtgtac gcgaatcact tgaagtacaa acaatgaaca tggctaaaag
      301 tgtggcagat ttgatttcaa gcacattgaa atatccagat ggggaacctg tggaatgcgt
      361 gatttctcca tctaccattg gtcgtgtacc agaggctgca gcttcccatg agttgttcaa
      421 aaaatcaaat gtttgcgcaa caattacagt tacaccatgc tggtgttatg gtagtgaaac
      481 tatggatatg tctccagata ttcctcatgc tatttgggga tttaatggga cagaacgccc
      541 aggagctgtc tatcttgcag ctgtactagc ttcatatgct caaaaaggga ttccagcctt
      601 tgggatttat ggtagagacg tccaggaagc tagtgataca gctattccag aagatgtcaa
      661 agaaaaactt ttacgttatg cgcgtgcagc tcttgcaact ggcttgatga gagacactgc
      721 ttacctatca atgggtagtg tttcgatggg gattggcggt tctattgtaa attcagattt
      781 cttccaagaa tacttaggaa tgcgaaatga atcggtagat atgacggagt tcacgcgccg
      841 tatggaccgt ggtatttacg accctgaaga gtttgaacgt gctatggttt gggtaaaaga
      901 acatatcaaa gaaggagttg accgaaatcg tgaggatttg attctttcta aagaagagaa
      961 agaaaaacaa tgggaatttg ttattaagat gttcatgatt ggacgtgact taatggttgg
     1021 taacccaaga cttgctgaac ttggttttga agaagaagca gttggtcacc atgctttagt
     1081 agctggtttc caaggtcaac gccagtggac agaccatttc ccaaatgggg actttatgga
     1141 aactttcctc aatactcagt ttgattggaa tggtattcga aaaccatttg tatttgcgac
     1201 agagaatgat tcactaaatg gtgtgtctat gctctttaat tatctattaa caaatactcc
     1261 acaaatcttt gctgatgtgc gtacttattg gagtccagag gctgttaaac gtgtaacggg
     1321 acatacttta gagggttgtg ctgcagctgg cttcttacat ctaatcaact ctggttcttg
     1381 tacattggat ggtacagggc aagctactcg agatggcaaa cctgttatga aaccattctg
     1441 ggagttggaa gaaagtgaag tgcaggctat gcttgaaaat acagacttcc caccagcaaa
     1501 ccgcgaatac ttccgtggag gaggattctc aactcgtttc ttgacgaagg gggatatgcc
     1561 agtaacaatg gtacgtctca atcttctaaa aggggttggt ccagtgctac aaattgcaga
     1621 aggttataca cttgaacttc ctgaagatgt tcaccatact ttagataatc gtacagatcc
     1681 aggatggcca actacttggt ttgctccacg tttgacagga aaaggtgctt tcaagtctgt
     1741 ctatgacgtc atgaataatt ggggagctaa tcacggagcc ataacatatg gacacattgg
     1801 agcagacttg attaccttgg cttctatgtt gagaattcct gtcaatatgc ataatgtacc
     1861 cgaagaagat atctttagac ctaaaaattg gtccttattt ggaacagaag atctagaatc
     1921 agcagactat cgtgcatgtc agttattggg accactacat aaataaaact tgtttatata
     1981 ggaggtgaac ttacgtccct cctatccttt taaaaagatt tgttaaacaa ttcacaaata
     2041 attgaaaacg aatacaaaaa gtaatataat gatgttaaat agatagcgcg gaggcgcagg
     2101 aggaaaatta tatggctaca ttttatgttc cggcagtcaa ccttattgga aaaggtgttg
     2161 taaatgaagt gggtccttat atcaaggaac ttggctataa aaaggcactt ttggttacag
     2221 ataagtacat cgaaggcagt gatattttac ctaaggtttt aaaaccactg gatacagaag
     2281 gaatcgaata tgtcatcttt agcgatgtag agccaaaccc tacttgtaaa aatgtcacag
     2341 atggggtagc tgctttgcaa gaacatggct gtgactttat catcagtctt ggcgggggtt
     2401 ctccacagga tgcagctagt tgtatttcta tcatggctac aaatggtgga aaaccacagg
     2461 attatgaagg gcttcataag tctgctaaaa aaggcttgcc agttgtggct atcaatacaa
     2521 cggcaggaac ttctgcagaa atcactatta actatgtgat tactgatgaa gaacgcaagg
     2581 ttaagatggt aatggttgac aagaatagcc ttgctcttat ctctgttaat gacccggaac
     2641 tcatgctttc caaacctaaa ggcctgactg ctgctactgg tatggatgct ctgactcacg
     2701 ctgttgaagc tttggtaaca cctggtgctt atgatgtaac caagaaactg tctattggtg
     2761 ctattgagct tatcaaggaa tatcttcctc gtgctgtaga aaatggacat gatattgaag
     2821 cgcgtgaagg tatggtcaat gccatcttcc ttggtggtat gagctttaat aatgctggtc
     2881 ttggttatgt tcactcaatg gctcaccaac tcggtgcagt atataatttg ccacatggtg
     2941 tttgctgtgc catgttgcta ccagttatag aacgtgaaaa tgctaaacgt gtaccagaag
     3001 ctttccgcaa tgttgccaaa gccttgggac ttcatgtaga aggtaaatca gatcaagaat
     3061 gtgccgatta tgcgattgtt gagattgaga agctttctga gacagtaggt attcctaaga
     3121 aactgactga acttggtatt gaagaaaaag atttcgactt tgaatacctt tctaagaatg
     3181 ccttgattga tgcctgcgca ccaggaaatc catttatgcc aaccttagaa gaaacgattg
     3241 ccttttataa agagttattt tagcaagaga ttggacgaca ataggacaat tcacctctaa
     3301 ttattgacat ttcaggaaaa atcgcagtaa aaacggcaca gccttcttta aatgtgctat
     3361 aatactagaa aaatacttgt ggaggttcca ttatggcaat atttttcatg atttttctga
     3421 ttgtttgtgt gctcctattg gtgatagtca cactgagtac agtttatgtg gttcgtcagc
     3481 agtcggtggc gattattgaa cgctttggga aataccaaaa ggttgccaat agcggtattc
     3541 atattcgctt gccttttggg attgactcga ttgcagcacg gattcagttg cgcttgttgc
     3601 aaagtgatat tgtggttgag actaagacca aggacaatgt gttcgttatg atgaatgtag
     3661 cgactcagta ccgtgtcaac gagcagagcg tgacagatgc ttactataaa ctcatacgtc
     3721 cagaatctca gattaaatct tatatcgaag atgctcttcg ctcttctgtt ccaaaattaa
     3781 ccttggatga attgtttgag aaaaaagatg agattgccct tgaagttcaa caccaagtag
     3841 cagaagaaat gaccacttac ggctacatta tcgtgaaaac cttgattacc aaggtcgaac
     3901 cagatgcaga agttaagcaa tccatgaatg aaatcaatgc ggcgcaacgt aagcgggtcg
     3961 cagcacaaga attggcggaa gctgacaaga ttaaaattgt cactgcagct gaagcagaag
     4021 cagaaaaaga ccgccttcat ggtgtgggga ttgcccaaca acgtaaggcg attgtggatg
     4081 gattggcaga gtctatcacc gaactcaagg aagccaatgt tggcatgaca gaagaacaaa
     4141 tcatgtctat cctcttgacc aaccagtatt tggatacctt gaataccttt gcctctaaag
     4201 gaaatcaaac catcttttta ccaaatacgc caaatggtgt ggatgatatc cgaacacaaa
     4261 tcttgtcagc ccttcgcgct gagaagaaat aatagactaa tactcttcga aaatctcttc
     4321 aaaccacgtc agcgtcgcct tgccgtatat ttgagctgac ttcgtcagtt ctatctgcaa
     4381 cctcaaaaca gtgttttgag caacctgtgg ctagtttcct agtttgctat ttgattttca
     4441 ttgagtacaa agagcttggc aacaggctct ttttgtattg cgtttattaa gaaaaacaaa
     4501 aaacatagat atacatttaa ctaaaaatta ttgtatgtta cctcccttaa ttaggaatga
     4561 taagggaata actagaaaga tttgtgaata caaactattt ctgatataca aaatatacag
     4621 taataatgaa tgatgggaga tgggatgaaa gaatttcaat ttgagagaaa gcagcgtttt
     4681 tctttgagga aatatgcaat aggagcttgt tcggtcttgc taggaacgag tttatttttt
     4741 gctggtatgg gtgatcagcc tgtacaggat acagaaacga gttcagcact aatttcaagt
     4801 cattatttgg atgagcagga tttatctgaa aagctgaaat ctgagttgca atggtttgaa
     4861 ttagaaaaca agcttttgaa cttatgggag cattaggtta ctatgaagga tttgttcctt
     4921 atgtttcaaa tcaatacaaa aaccaagctg aagaagaagg caaaccgcta tctgataaat
     4981 atattttcga aaaaatctta ggaaaaacat atgcagcctt caaaaaagat caaattaatg
     5041 agcgtgtcga gaaattaggt aagttaaaac ctattacaat aaattacaac ggaaaatcag
     5101 aagtaattga tagtaaagaa aaattacaag agcttatgaa taaagccgtt aaagacgaag
     5161 tggctcaaat atagtaaaat gaaataagaa taggacaaat cgttcaggac agtcaaatcg
     5221 atttctaaaa atgttttaga agtagaggtg tactattcta gtttcaatat actataaaat
     5281 caggcaatac cacggctaaa aagtttgagt ttatcgaaac accagtgcaa aaactgaaaa
     5341 aatcgattta taaagctcat ctcaaagatt cagatgactt tagaccagaa acatccacac
     5401 aaatcttttt gaaagctgtt tgaagctttg tccttgcttt cttagttcat agatctgaac
     5461 tttatcttca taactcaatt tcataataaa acaccccaaa agttagattt attctgtcta
     5521 acttttgggg tgcagttcat taatcggtcg attttctttg gtagaaacag ttgcctgatt
     5581 aagttgcaag tcgatagacg gcttcggcat aaatatccat agcacggtac aaatcttcta
     5641 gaacggcaca ttcatttgcc tgatgttctg tctgcttcgc tcctgggaat aaggcgccga
     5701 aggctacaca atttggcatg gtgcgagcaa aagtggcacc accggatgaa atagcaggac
     5761 tgttatcgcc agtcttttct tggtagattt gcatcagtgt gctaacgagt ttactgtctt
     5821 ttgcgacgta tagaggcgct agatagtcaa actcttcgta gcggagttgg tagttttgtg
     5881 cacatcttgt aagcaactct actagttctt ccttgtcagc taagacagga gtccgaatgt
     5941 caatatggat ttcagaacgt ttatgattga tcatgagacc tgcgacatta aaggataggt
     6001 gaccagaagg ttcatctgct atatcaccaa agatttgtct tcctgtgccg tcttgacctg
     6061 cttgtgttgc aagaaagctg agagcagggt gttcttggag aggagcaaga atggtagcta
     6121 gtcggatgac agcattgata ccttgactag catccttagc atgctttggc actccgagaa
     6181 ccgttacggt ttgttcagtg gtttggtaat cataaccagc ttctttgaga ccgttacaaa
     6241 cctgttcata gaggggacct tggtagttgg ccttgtctgg tacaacgtta aaggcgcctc
     6301 ctacttcaag ctctagttga tccgatccag ggccatgaag tttgacctgt agaagccctt
     6361 tttcagcata ggtcagagga aaagatgagt caggtgcaaa gcccatgctg gcctgttctt
     6421 cgatggtatt gtagcgtgcc atgcagcgcc agagggtttc ctcatcggta ccaaagataa
     6481 agcgtacgcg ctttttgaac tgaatacctt ggtccagcaa gctttttact gcatagagag
     6541 ctgcgagcga agggccttta tcatcttgga caccacgtcc gaatacccag ccgtctttga
     6601 tagttgcttc aaatggcggt gtctgccaat ctgcttcatc acctgatgga acaacatcca
     6661 aatgacagag aatggccaga agctctgctc cctgaccgat ttctgcatat ccgtaataac
     6721 ctttagggtc aagataggta gtgaaaccta tgtctcgaca aatctctaaa gttttttcta
     6781 ggacatcttg gattgcttgt ccaaaaggtg ttccattttc tccttcattg agtactgaag
     6841 gataggaaat caaggttttt aatgatataa gaaattcatc tttaacttga tttgttatat
     6901 ctattttcat ggaatcacct cactcactat tttataggaa tggaaggaag gttccaagga
     6961 gaagaagggc gatggtcact acaataatag cgactacgag tttgcccatg aatttccacc
     7021 aagtaccaat gttgatacgt ccaagtgcaa gagctcccat cacaatacca gatgttggtg
     7081 caatcaagtt caagacacct gaagcagatt ggtaagcagt gataatcaag ctaggacgga
     7141 catttacaaa ttctccaagt ggagccatga tacccatagt tgcgctggca agaccagatg
     7201 aagatgggat caagaatgac ataggtagat agaagatata agttacaacg ataaagactt
     7261 gtgaagatag accgctcaag ccttctttac cccagttgag gattgtatcg gtaatcatac
     7321 cgtcgttcat gataacttga ataccacgag caatcgctac gatcaaggca acactgagca
     7381 agtcagcagc accattcatg aaggaagaga taatcttatc ttctttaaga ccataaataa
     7441 caccaatcag gatacccata aaggcaaaga gcattgcgcc ttctgggaag taccaagtac
     7501 ctagtgcaga agtagatgaa ccgacaatat taccaataac tggaagacca gtcaaccaag
     7561 cattaaagtc atcaaaaatg gtaacgccaa ggtctgtcca tggaatgaag ctcaatacca
     7621 tcaagatgaa tgtcaacaca aataagaaga gaactgattt ttgtttgctg ctaagtgtag
     7681 attctacaga tgaagattct tctacgttaa agtgtttcaa atcttctttg cgagtactat
     7741 aaaccagtga cttagtcgga tctttttgaa tcttatccgc ataacggtaa acaaaccaag
     7801 tactaagagc agtcaaggta acccagaaga tcagacgaag tacgataccg tcccctgtac
     7861 caactcccgc agtcgctgaa gcaatacctg tcgcaaatgg attcagagta gatgccaaac
     7921 agccgatttg agaaccgagc aaaataattg caacaccagt caggctatca aaaccaacgg
     7981 ccatcataac tggcacaagg agtggataga aggccattgt ttcttccccc ataccataag
     8041 ttgtaccacc gagggcaaac aaaggcatca gtaccaaaat taacattttt tcgcggccct
     8101 tatacttctt cacgatagag gcaatcccta cgtcaagagc accagttttg ttgacaatgc
     8161 caaggaaacc cccaaccata aggatgaaga aggctacatc aatcgctgcg ctcgtttctt
     8221 taatgagcga accttcctct ggatgagtac ctagcatagc ccgaatcggt gccatgagga
     8281 catcccaaat cccttgtgga ttttgaggct gagtctcgta aataccttct ataaaggccc
     8341 ccgcagggat aaaccaagtt agcactgcca taatagcaat gattatcaat aatacggtgt
     8401 aagatgaagg catcttaaac ccttttttag ctttttcact catttgtttt tcctcctgat
     8461 gtctaacaaa gtgtaaaaaa ccaactactt tcttaaataa acattcaaga aaacgctttc
     8521 tatttatatt tgttttacca tatta
//
LOCUS       DAVLWA010000078         8494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLWA010000078 DAVLWA010000000
VERSION     DAVLWA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.078"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            68..1786
                     /locus_tag="VYN80_001654"
     CDS             68..1786
                     /locus_tag="VYN80_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1541534.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            1897..2244
                     /locus_tag="VYN80_001655"
     CDS             1897..2244
                     /locus_tag="VYN80_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1541535.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(2522..3358)
                     /locus_tag="VYN80_001656"
     CDS             complement(2522..3358)
                     /locus_tag="VYN80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1541536.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3371..4000)
                     /locus_tag="VYN80_001657"
     CDS             complement(3371..4000)
                     /locus_tag="VYN80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1541537.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASELLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(4010..4651)
                     /locus_tag="VYN80_001658"
     CDS             complement(4010..4651)
                     /locus_tag="VYN80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1541538.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(4797..6026)
                     /locus_tag="VYN80_001659"
     CDS             complement(4797..6026)
                     /locus_tag="VYN80_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1541539.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(6016..7182)
                     /locus_tag="VYN80_001660"
     CDS             complement(6016..7182)
                     /locus_tag="VYN80_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1541540.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7326..8339
                     /locus_tag="VYN80_001661"
     CDS             7326..8339
                     /locus_tag="VYN80_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1541541.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEKGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         2715 a         1864 c         1505 g         2410 t
ORIGIN      
        1 ttactaaaac taaaaaaaat gatataatta aactgattaa atgttttaaa aataaaggag
       61 aatttgaatg tcttaccaag aaaattacca gaaatgggtt gattttgtgg agcttcctga
      121 ctaccttcgt caagatttgg aaaatatgga cgaaaaaact aaggaagatg ccttctatac
      181 aaatcttgaa tttggtactg caggtatgcg tggcttggtt ggtgctggta caaaccgtat
      241 caacatctac gttgttcgcc aagctactga aggattggct cgtttgattg agtcaaaagg
      301 tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat agccgtcact tctcacctga
      361 gtttgccttt gaatctgcgg cagttcttgc taaacacggc atcaaatctt acgtatttga
      421 aagccttcgt ccaactccag aactatcatt tgcagttcgt catctcaact gtttcgcagg
      481 tatcatggtc acagccagcc acaaccctgc tccatttaac ggttacaagg tttacggtga
      541 agacggtgga caaatgcctc cacacgatgc ggacgctttg actacttata tccgtgcaat
      601 cgaaaaccca tttgcagttg aagttgctga tgtggaaact gaaaaagctt ctggcttgat
      661 tgaagttatc ggcgaagctg ttgacataga ataccttaaa gaggttaagg acataaacat
      721 caacccagcc ttgattgaag aatttggtaa agacatgaag attgtctaca caccacttca
      781 tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa gcaggatttg actctgttca
      841 agttgttgaa gcgcaagcaa ctgctgaccc agacttttca actgtaacat ctccaaaccc
      901 agaaagccaa gcagcctttg cccttgctga agaacttggt cgtcaagttg gtgcagatgt
      961 tcttgtcgca actgacccag acgctgaccg tgttggtgtt gaagttcttc aaaaagatgg
     1021 tagctacctc aacctttcag gtaaccaaat cggtgctatc atggctaaat acatcttgga
     1081 agctcacaaa aacgctggaa ctcttcctga aaatgccgct ctctgcaaat ctatcgtttc
     1141 aactgacttg gtaacgaaga ttgctgaaag ctacggcgca actatgttca acgtcttgac
     1201 aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa gaaaaacaca accacactta
     1261 catgatggga tttgaagaaa gcttcggtta cttgattaaa ccatttgtac gtgataaaga
     1321 tgccatccaa gccgttcttg tcgttgctga acttgctgcc tactaccgtt ctcgtggttt
     1381 gacacttgct gacggtatcg aagaaatcta taaagaatat ggctactacg cagaaaaaac
     1441 aatctctgtt actctttcag gtgtcgatgg tgctgaacaa atcaaagcga ttatggctaa
     1501 attccgcaac aatgctccaa cagaatggaa cgcaacagct atcactgtcg tagaagactt
     1561 caaggcacaa actgctactg ttgctgacgg tactgttaca aacttgacaa ctcctccaag
     1621 tgatgtgttg aaatacacac ttgctgacgg ttcatggatt gccgttcgcc cttcaggtac
     1681 agaaccaaaa atcaagttct acattgcagt tgtaggggaa accaacgaag aatcacaagc
     1741 taagattgct aacatcgaag cagaaatcaa tgcatttgta aaataaagac ctataaaaga
     1801 tgagaccaac caacctcatc ttttttcgta tcaaatctaa gcaattttag aaactttatg
     1861 gcatactaga cttatcaatg aaagcgaggt aatctcatgg aacaattttt ggataacatc
     1921 aaagaccttg aagtcactac agttgtgcgt gcgcaagaag ctcttgataa aaaagaaact
     1981 gcaaccttct ttatcggtcg caaaacttgc ccttactgcc gtaaatttgc agatacattg
     2041 tcaggtgtcg tagctgaaac caaagctcac atttacttca tcaatagtga agaaccaagc
     2101 caactcaatg atttgcaagc attccgctca cgctatggaa tcccaactgt accaggtttt
     2161 gttcatatta cagatggaca aatcaacgtc cgttgcgact cttcaatgtc agcacaagaa
     2221 atcaaagatt tcgcaggatt gtaaaaaatc agctaacaag tcttaaaatc acaaaaacgc
     2281 ataatgtaag gtgcaaaaac cttgatatta tgcgttttat tgttcctaaa acagttacat
     2341 cagcatttca ttacagaaca ttttaatttt ggaagtcaaa tcaattttta acaatgtttt
     2401 agaaatcaca gtgggctatt ccatattcaa ttatccatat tcaatcgcta ttatcgtttt
     2461 cctagattac aaaaaaacta cgttttgcta atcaaaacgc agtttcagcc actattttat
     2521 cttactgtcc ttcttttact tctttggttg ctacatcttc tccaaaccat ttttggctga
     2581 tttcttggaa cttgccgtcc ttgtaaagac tagaaaaagc ttcatttatc ttcttaacca
     2641 aggttgtatc ttccttacgg gctccaaccg caaaagcttc tgtttctagt ccaactgtaa
     2701 agacattata atcgtttaaa acaccttctg cttctaaata atagtttgca tagacacggt
     2761 caatcaatag accatcaatt cgatcgtttt tcaaatcaat caaggcttca ttaaaggttt
     2821 ggtattgatt cgcttcctta ttagcgacaa tattcttcaa aatttctgga tttgcttcaa
     2881 agtccgcata accagatgaa ccagcttgag ctcctaatgt ctttccagtc atatcctttg
     2941 cagtcgtgat accagatgat ttcttcgtaa ccaatacctg ctcattcttc atatatgagt
     3001 tactgaaagc caccttttca cggcgttcgt ctgtagcgga atagccattc caaatcagat
     3061 caatcgttcc ttttgtcaat tcagcttctt tcaaatccca atcaatcggt tgccaattta
     3121 ccgtgattcc gtatttttca aaaacagctg tagctaaatc aatatcaaat cctgcataag
     3181 aaccatcttt ctgagcaaat cccattggaa caaaagtact atcaaatcca atagtaatag
     3241 acttgttaga ctggtacttt gaccaattat ctccactagt ttcgctagaa tttttcccac
     3301 aagctactaa gaacaaagca gtcatcagac tgactaatac aagcatccat tttttcatcc
     3361 atttttcctc ctatttaggt tctactttca ataatacatc tgcgatattt tcagcaaact
     3421 gcaaatcatg ggtaaccaca atctgggtca tcccaagttc cctattttgc aagattagct
     3481 tctccacttc caaacgtaat tctggatcca gggcagaagt tggttcatcg tagccaatga
     3541 tttctgggtc aatcatcata gcacgcgcca aagccacccg ctgcttttgc ccaccagata
     3601 gtgagaaagg ataggcctct gcgtgtcctc ctagtcctaa ctgttccaag agttcactcg
     3661 ccttcttctc agcctcttcc tgcttcattc ccatggtctt cacaggcgat aaagtcaaat
     3721 tttccagaac tgatagatga ggaaatagtt gaaaatcttg gaagacaaat cccagtaggt
     3781 tgcgcttctg caattcatcc agctctaaag gttgtccatt ataaaagatt tgccctgaat
     3841 caatggtttc aagacctgca agcatacgta aaagagttgt cttacctcca ccagaaggtc
     3901 caacgatagc caggatttgc ttttcaggaa tacttagact gaaatcagac aggatttgtt
     3961 tgtctccaaa gactttattg atatttcgta attctaacat agctgccccc tatctataat
     4021 aactgtactt tttctcaact tttttggaaa taattgtcac aatcccaatt aaaatcaagt
     4081 aaatggctcc tgccaagaac ataggaacca gactagcatc gcggttagca gctgttcgac
     4141 tagccaagat aaggtctgaa attccgagag catagaccaa agaagtatcc ttgaccaaac
     4201 tcataacttc attaaagaca ctaggaagaa cgatcttggt cacttggggc aagataatat
     4261 agcgcactct gtcaaaaggg ctaaacttca agaccttggc agcctcatac tgtcctcttg
     4321 gaatagtgtc aattccccca cggaaaattt ctgcaaagta agctgcatag ttgagaacaa
     4381 aggcaataat agctgcagga aggcggtcta aacgaatccc aatacttggg agcacataat
     4441 agataaaaat cagttgcaag agtaaggggg ttcctcgcat aacccagatg taaatgttaa
     4501 tcagataatg gaggggcttc caatggactt gcaaggcaaa ggcaatcaaa acgcccaagg
     4561 gaatcgaaaa tagcaagacc agtgcaaaga cctgtacagt cgtgctcgca ccattcagta
     4621 aactcggtaa tatctcaaat aaataagaca tactgcacct cctaaaagta attgttccta
     4681 ttatagcatg aataaacaag attaccaagt atttttgtaa aaagattcta ccatatacaa
     4741 ataaaaaccg tagctatcaa aagagataaa ctacagttct atatgtttaa acattcttac
     4801 aatctctcaa acagttcttg catttgcaca tcatctggaa ccagttttaa gtaagtgtga
     4861 gcatggactt ttgcttcttc aaaatgtccc aattcacgca agagatagat atagtgttcc
     4921 agaaattctg gattgtcctt caaatctcct gtcaactctt gataatgctc ataagcagta
     4981 tccaaatcgt ccatttcttg ataagaacga gcaatcatcc acttggtcaa aagattttct
     5041 ggctcctcac tctgcaagtc tagaatatcc tcataacgct cctgctccag ataaatagtg
     5101 gctaaacgaa gcaagatttc ttctgtatcc tcagcgtctt cttttgcagt aaggagataa
     5161 ttttctgcac cactagcatc atgcaattca taagaaaatt gtgaagcagc tagcaagagg
     5221 cgagtttcaa agggattttt ctctaatcct tgcttagcga tacgcagggc ttcttgaact
     5281 tgatgttcct tatgtaaagc ctgactgtac ccatactcat agccttcaaa gtcaggagaa
     5341 atggtatcaa gctgcttaaa gtagagggtg gctttttgat attcttcttg atcaaaataa
     5401 agactggcca actcaaaagc tgttaagtca tcgtattcta actccagggc tttttctaaa
     5461 aactcagtag ccgtttcaaa tttccctaac tgagcatagg caaagccaat tcgttgatag
     5521 gtggaaatgc ccgtttgctc ataaatcgag cgattatcta actgggcata ggcttgaata
     5581 gccgcttggt aattttccaa ctcactatcc aactctgcca aacccaatat caagagagaa
     5641 tcctctgagt aggtcaaggc ctccaataat ttctcacgtg ccacatctgt caaaccttcc
     5701 agctggtaaa ggtctgcctt caaaaccaaa gacgagacat accagtcact gtcagcttgg
     5761 atttcctcaa gataggtaaa ggcttcttct atttgaccat cctcgctagc aattgcagct
     5821 agattaagat gaacctctgg aaaatcctct acaattttca ggtaaatttc cttggcctga
     5881 ggatagaaac caatcccttc aagataagtt gccaactcat acagaagatt acttgaatca
     5941 ttttctaaag ctttggcgaa ataatgctca gccttagtta aatcttgctc ctccaaagcc
     6001 tgtaacatct gttgactatt gttcactctt gacttcctca ccctctaatt catatagacc
     6061 actgactacc ttataccatt cgaaaatggc tgaaatgaca accttagcag aggcataaac
     6121 cggaatacca agtaaaactc cccagatacc aaacatagat cctgaagtta acaaaacaaa
     6181 gagaacatta atagggtgga tgtttaattg acttcccaaa atcaatggag agacaaaacg
     6241 gccttcaata gtttgttcta cgataaagac aatcactact ttcaaaagca tgactggacc
     6301 agcaatcaaa cccaatacta gagcaggaag catggctaga aagctaccaa gataagggac
     6361 cagatttaaa ataccagcag taacccccag cgtaaccgca tagcgtagac caataatctt
     6421 gaagaagatg ataaacatta ctgctacaat aatagccact gtcacttgcc ctcgaacata
     6481 gttggacaac tgttgattca catctgatag aacttgtcca acaggttcct tcaattttct
     6541 tggaatgaat tgggtcaaat agttacgcaa gcctttccca tcacgcaaga gataaaagag
     6601 catgaaagga acgataatca aggcaacaat cacttgagaa gctccgctaa taaaggcact
     6661 cacccagttg actgcctgag atgaaacctt acttgccaaa actgtagcct ggctagaaaa
     6721 attggtcaaa acttgctcta attgaggtct gaaatcatct ggcaggtgct gggctaccaa
     6781 tccattaaca atcctatcta tatcttctaa gtaaacagga acgtttcttg caaaggtcaa
     6841 aacctgacgt tgcagatttg gaatggcgac tgccaagccc caaatgataa agagagcgat
     6901 gataacaaag acaatagtga tagctataac acgattaacc ttatgcttct ccatccaatc
     6961 aacaatagga ttcaacaaat aatataacaa accagacaaa atgactggca acatcacaac
     7021 tgctaaaaag tctaaaacag gtgaaaatag aaaactaatc ttacttaaaa taaaaagatt
     7081 cagtcccaat aataaggtta ctaaaaatac cgtaattgcc ttgttatcta aaaaccactt
     7141 gaaaaaccaa gataggctaa aatgtttctc tttttgctcc ataaatactt cctttctatt
     7201 gttctctcat tataccattt ttgaaacaaa ataactcttt tttaaagtgc ttacatctaa
     7261 ttggatatct ttaatttact tgtttttgtg atataatagt cttatcttta cacagaggta
     7321 tagtaatgaa agaaactgtt tattttggaa cttatacacg tcgtacttct caagggattt
     7381 acaaggcaga ctttgataca gaaactggtc agctttcaaa tctagaactt tttgcagctg
     7441 agccaagtcc aacctacctt gcctttgacc agcaccaaca tttatacact gttggtagcc
     7501 aagacgataa ggggggaatt gcagcctatc aaactgacgg gactgtgtta aatcatgttg
     7561 ttgaaaaagg agctccccac tgttatgttg ctgtcgatga aaagcgtgat ttggtttacg
     7621 cagccaacta tcacaaggga caagtccttg tttataaacg ccaggaagat ggtagtcttc
     7681 tacttagtga tatggatcaa cacagtggcc aaggtccaca tgaaaatcaa gcttcccccc
     7741 atgttcacta tacagattta acacctgacc actatctagt gacctgcgac ttgggaactg
     7801 accaagtcat cacctatgac ctcgatcaag aaggaaaatt atctaagctc tatacctatc
     7861 acagcaagcc aggagcaggc tcacgccata tcattttcca taaccactat aaaatcgctt
     7921 atctcatttg tgaactcaat agtactatcg aagttttaat ctacgatggc gttggcgaat
     7981 ttgaacgtat gcaggtcatt tcaactttac cggaagctta cgaaggcttt aatggaaccg
     8041 ctgctattca tctctctaaa gacggtaaat acctctacgc ttctaaccgt ggccatgatt
     8101 ctatcgcagt atataccatc cttgcggacg gtagcttaga gttattagaa atcgttccaa
     8161 cgcatggtca gactccacgt gattttgatt tgacacccga ccaaaaattt ctcattgttg
     8221 tccatcaaga ctctgacaat gcaactgtct ttaaacgtaa ttgtgacaat ggtcgtctag
     8281 cagaactctc caacgacttc catgttcccg aagcagtctg catccgtttt gctccttaaa
     8341 acaccataaa aaatggttct ataatatttg tagtgggtaa atcccctatg gatattatgg
     8401 agcctatttt tgtgtagaaa aaaagtccca tatgacctat aatgaaaagc gacaaaacaa
     8461 ctcattagaa agaatcatat ggaacaatta catt
//
LOCUS       DAVLWA010000079         8426 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLWA010000079 DAVLWA010000000
VERSION     DAVLWA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.079"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            8..1009
                     /locus_tag="VYN80_001662"
     CDS             8..1009
                     /locus_tag="VYN80_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1541542.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1617..1694
                     /locus_tag="VYN80_001663"
                     /pseudo
     CDS             1617..1694
                     /locus_tag="VYN80_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2150..3511
                     /gene="trpE"
                     /locus_tag="VYN80_001664"
     CDS             2150..3511
                     /gene="trpE"
                     /locus_tag="VYN80_001664"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1541543.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3508..4074
                     /locus_tag="VYN80_001665"
     CDS             3508..4074
                     /locus_tag="VYN80_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1541544.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4085..5089
                     /gene="trpD"
                     /locus_tag="VYN80_001666"
     CDS             4085..5089
                     /gene="trpD"
                     /locus_tag="VYN80_001666"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1541545.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5086..5853
                     /gene="trpC"
                     /locus_tag="VYN80_001667"
     CDS             5086..5853
                     /gene="trpC"
                     /locus_tag="VYN80_001667"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1541546.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5840..6439
                     /locus_tag="VYN80_001668"
     CDS             5840..6439
                     /locus_tag="VYN80_001668"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1541547.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLPQSFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            6417..7640
                     /gene="trpB"
                     /locus_tag="VYN80_001669"
     CDS             6417..7640
                     /gene="trpB"
                     /locus_tag="VYN80_001669"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1541548.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7633..8409
                     /locus_tag="VYN80_001670"
     CDS             7633..8409
                     /locus_tag="VYN80_001670"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1541549.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIAGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
BASE COUNT         2489 a         1585 c         2033 g         2319 t
ORIGIN      
        1 gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc
       61 aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga
      121 aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc
      181 aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc
      241 ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt
      301 tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta attcaattat
      361 atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat
      421 cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac
      481 gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc
      541 tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg ggaggagaat ctcatgttaa
      601 taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt
      661 aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt
      721 gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt
      781 gattcgtgcg tgctatgaga aaggaattct tattccagat gatatttcta ttattggatt
      841 tgataatatt cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta
      901 tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt
      961 cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat
     1021 tcttgaaaat cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat
     1081 tcagtttcta ttgagttgga tccaatttct aacaagtggg acatgttcat gttcgatcta
     1141 aggaagacat ggtcatattc gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa
     1201 agaaaaaaca aagtattaaa ataagttcaa caaatcagaa aagtaactat ttttgatctt
     1261 cagggagcct gtggtgattg tgaataggtg gttggaagta gtgaaagtgg gctgattttg
     1321 aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct tacagtgcag cttgttgtta
     1381 aacaaggcag agatgtaaag ggatagtccc tttataattg aggtggcacc gcgttaccaa
     1441 cgccctcaca cggaagtata ttctgtgtgt gggctttttt ctatccgtcg tttggtttat
     1501 cttttattag ggccttaagt cgctttgatg aacttgagtt ctatctacag ctccatgcct
     1561 actactaaaa gcaaacaaaa aggctggact aatacgaaaa aagctagtaa tactcaatga
     1621 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaaacactgt tttgaggttg
     1681 tggatagaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggtttg
     1741 aagagatttt cgaagagtat aaaggtgtac cgagatgaac atctcttcat caatgtctct
     1801 ggtcgtggag gcaaggacct agctgcgatt gcagattact agaagctaaa aaataataga
     1861 tggaaaaatt acagttcttt ctctcacaga gagcttgtgg ttgctgaaaa caagcagaga
     1921 aagctgtaaa gttgggttca tctgaaacga gagaagaaaa catcattctc aagggagtgc
     1981 cctttatcgc acagcctgtt acaggagata tctgagacag gaatgagaga taggagaaat
     2041 cctataattg aggtggcacc gcgaatttcg tcctcacgca agttattttg cgtggggatt
     2101 tttcatacag tcaccacgga ctagaagtag aactgaaagg gaaattacaa tggaacgaat
     2161 cattcatgga gatgtcttat caccaatctt ggcttatatg cgcttaaagg ggcaacataa
     2221 ggttatttta gagagtattc cgagagacaa ggaaacagct cgtttttcta tcctagccta
     2281 taatccagtt tttgagatta agtttgaaaa tggggttctc tatcaaaatg gtcaagtgat
     2341 tgatcgggat cccttggatt tcctttatga agtgattcat aagagtcagc accactcaga
     2401 gctacctttt ggtggtggag ccattggttt tgtgggttac gatatgattt cgctttatga
     2461 agaaattggt caaatccctg aggatacaat tgggacgcca gacatgcatt tctttgtcta
     2521 tgagagctat atggtctttg accacaagaa ggagaagata catgtcatag aggatgcact
     2581 ttatagtgag cgcagtcaag aggccttgga aaaatccttg aaccaagtgc ttgaggaatt
     2641 acgcattcct gctccaaatg aatttgaaga tttggatcta tctccgttag acttcaaacc
     2701 gcatatcgct ccacataagt ttgagggaat ggtagaaaca gctcgtgact tgattcgtaa
     2761 cggagatatg ttccaatgtg tactcagcca gcgtttttca gcagaagtta ctggaaatcc
     2821 atttgacttc tacagaaatc tccgcgtgac taatccttct aattaccttt atttctatga
     2881 ttttggggat tatcaaatca tcggagctag tccagaaagt ttagtttctg tcaaaaatgg
     2941 catcgtgaca accaatccga ttgcagggac gcgaccaaga ggggctacgg atgaagaaga
     3001 caagaccttg gcgactgacc tcctctctga tgagaaggaa acagcagaac atcgaatgtt
     3061 ggtagacttg gggcgtaacg atattggccg catctctgaa acggccagtg tccaagttac
     3121 taagtatatg aaggtggagc tcttccgcta cgtcatgcat ttgaccagcg tggtcaaggg
     3181 gcgtttgctt ccagaactca cggccatgga tgccttgaaa gctacacttc ctgctggaac
     3241 cgtttctgga gcaccaaaga ttcgggcgat gagacgcatc tatgaactgg aaacggaaaa
     3301 acggggcgta tatgcaggag caatcggcta cttgtctgcg acgggtgata tggatttggc
     3361 cattgccatc cgaaccatga ttctcaaaaa tcaaagagcc tatgtgcagg ctggggcagg
     3421 gattgtctac gactctatcg cccaaaacga ataccaagaa accattaaca aagcaaaatc
     3481 tatgactaga attggagaac taagaccatg attttattga ttgacaacta tgattctttt
     3541 acctataact tggcccaata cattgggaat tttgcagaag ttcaggttct gagaaatgat
     3601 gattccaagc tgtatgaaga agctgaaaaa gcagatggtc tggtcttttc tcctggtcct
     3661 ggttggccag ttgatgctgg aaagatggaa gacatgattc gtgattttgc aggcaagaag
     3721 ccgattcttg ggatttgttt gggccaccaa gccattgcag aagtctttgg tggtaagcta
     3781 ggtttggctc caaaagtcat gcatgggaaa cagagcaata tcaactttga agcgccatct
     3841 gttttgtatc aaggtattga ggaaggacgt gcggtcatgc gttatcacag tattttgatt
     3901 gaggaaatgc cagaagactt tgaagtgaca gctcgttcga ctgatgacca agccatcatg
     3961 gggattcaac ataaaaacct accgatttat ggcttccagt accatccaga gagcattgga
     4021 acgccagacg gcttgtcttc tattcggaat tttatcgaga aggttgtaaa gtgaggaaac
     4081 taggatgaaa gagattattg aaaaactagc aaaatttgaa aatttatcag gtgtggaaat
     4141 gacggatgtc attgagcgta tcgtaactgg gcgtgtaact gaagcgcaga ttgcttctct
     4201 cctcttagct cttaagatga agggggaaac acctgaagaa cgcacagcca ttgcccaagt
     4261 catgagagga catgcccagc atattccaac tgagattcat gatgccatgg acaactgtgg
     4321 tacaggtggg gacaagtctt tcagttttaa tatttccaca actgcagcct ttgtcttggc
     4381 tggtggcggt attcacatgg ccaagcatgg taaccgctcg atttcttcta aatctggttc
     4441 cgcagatgtc ctcgaagcct tgggaatcaa tcttgacctc aaaccagctg aactaggtaa
     4501 ggtctttgat aaaactggaa tcgtctttct cttcgctaaa aatatgcacc cagctatgaa
     4561 atacatcatg ccagctcgtt tggaattggg aattccaacg attatgaact tgactggtcc
     4621 actgattcat ccaatggctt tagaaacaca gcttcttgga attagtcgtc cagaactcct
     4681 agaaagtaca gctcaggttt tgaaaaatat gggtcgcaaa cgtgccatcg tggttgctgg
     4741 accagaaggg ttggatgaag ctggcttgaa cggaacaacc aagattgcac ttcttgaaaa
     4801 tggcgaaatc agcttgtcaa gctttactcc agaggatttg ggaatggaag gctatgctat
     4861 ggaagatatt cgtggtggga atgctcagga aaatgcagaa attttgctta gcgttctgaa
     4921 aaacgaagca agtccattct tggaaacgac agtcttgaat gctggtcttg gtttctatgc
     4981 taatggtaag attgatagca tcaaggaagg agttgccttg gctcgtcaag tgattgctag
     5041 aggcaaggcc cttgaaaaac tcagactgtt acaggagtac caaaaatgag tcaggaattt
     5101 ttagcacgaa tcttagagca gaaggcgcgt gaggtggagc agatgaagct ggagcaaatc
     5161 cagcctctgc gccagaccta tcgcttggca gaatttttga agaatcatca ggaccgcttg
     5221 caggtaatcg ctgaggtcaa gaaagctagc cctagtttgg gagatatcaa tctcgatgtg
     5281 gatattgtgc aacaggccca gacttatgaa gaaaacggag cagtgatgat ttcggtgttg
     5341 acagatgagg ttttctttaa agggcatttg gattatctac gggaaatttc cagtcaggta
     5401 gagattccga cgctcaacaa agactttatc atagatgaaa agcaaatcat ccgcgctcgc
     5461 aatgcaggtg cgacagttat cttgctcatt gtggcagcct tgtccgaaga acgcctcaag
     5521 gaactgtatg actacgcgac agagcttggt ctggaagtct tagtggagac tcacaatcta
     5581 gctgaactag aggtagccca cagacttggt gctgagatta tcggggtcaa caaccgcaac
     5641 ttgactacct ttgaggtcga cttgcagacc agtgtagatt tagcccctta ctttgaggaa
     5701 ggtcgctatt acatttctga atctgccatt ttcacagggc aggatgcgga acgactagcc
     5761 ccatacttta acggaatttt ggtagggaca gctcttatgc aggcagaaaa tgtggcccag
     5821 agaatcaagg agttgcagat tgacaaaggt taaaatttgt ggactatcga ccaaagaagc
     5881 ggtggaaaca gccgtttcag caggagccga ctatatcggt tttgtctttg cacctagtaa
     5941 aagacaggtg actttagaag aggcagctga gttggcaaag cttattcctg cagatgtgaa
     6001 aaaggttgga gtatttgttt caccaagtcg ggtagaactg ctggaagcga ttgacaaagt
     6061 tggcttggac ttggttcaag ttcacggtca ggtggcagat gatttatttg agaatttgcc
     6121 ttgtgctagc attcaggctg tgcaggtaga tggagagggt catgtgccca attctcaggc
     6181 agactatcta ctctttgatg cccctgtggc tgggagtggc cagccctttg attggggtca
     6241 actggatacg actggattac ctcagtcttt ctttatcgca ggtgggctta atgaagacaa
     6301 tgtagtaaaa gcaattcaac actttactcc ctatgcagta gatgtatcaa gcggagtgga
     6361 gacagatgga caaaaagatc atgaaaagat tagaagattt atagagaggg taaagaatgg
     6421 catatcaaga accaaataaa gatggatttt acggaaaatt cggcggacgt tttgtcccag
     6481 aaacattgat gacagcagtt ttggagttgg agaaggccta ccgtgaaagt caggcagacc
     6541 caagtttcca agaggaatta aaccaacttt tgcgccagta tgtgggacgt gaaactcccc
     6601 tttactacgc aaaaaacttg acccagcata tcggcggagc caagatttat ctcaaacggg
     6661 aagaccttaa ccatacagga gcccacaaga ttaacaatgc cttaggacaa gtttggcttg
     6721 ccaaacgcat gggtaaaaag aaaattatcg cagaaactgg tgctggtcag cacggtgtgg
     6781 caactgcaac tgctgcggcc ctctttaaca tggaatgtac catctacatg ggtgaggaag
     6841 atgtcaaacg ccaagccctc aatgtcttcc gtatggagct tttgggagct aaggtcgagg
     6901 ccgtgacaga tggttcgcgc gtgctcaagg atgcggtcaa tgcagctctt cgttcatggg
     6961 tggctaatat agatgatacc cactatatcc ttggttctgc cttggggcct catccatttc
     7021 cagaaattgt tcgtgacttc caaagtgtca ttggtcgaga ggctaaacaa cagtaccgtg
     7081 acttgacagg acaaaatctg ccagatgccc tagtagcctg tgttggtggt gggtcgaatg
     7141 ccatcggtct cttccatccc tttgtagaag atgagtcagt agccatgtat ggagctgaag
     7201 cggctggact tggtgtggat acggagcacc acgcagctac cttgaccaag ggtcgtccag
     7261 gtgtccttca cggttccctc atggatgtgc tccaagatgc ccatggtcaa attcttgaag
     7321 ccttctctat ctcagcaggt ttggactatc ctggtatcgg tccagagcat tctcactacc
     7381 acgatattaa acgtgccagc tatgtccctg tgacagacga agaagccttg gaaggattcc
     7441 aactcttgtc tcgtgtggaa gggattatcc cagccttgga atctagccat gctatcgcct
     7501 ttgcggtgaa attggccaaa gaacttggac cagaaaagtc tatgattgtc tgcctatcag
     7561 gtcgtgggga caaggatgtg gttcaagtca aggaccgctt ggaagcagat gcagcaaaga
     7621 agggagaagc tcatgcctaa gacactaaca gaaaaattaa atgctattaa agcagctgga
     7681 aaaggaattt ttgttcccta tatcatggct ggtgaccacg agaaaggttt ggacggtctt
     7741 gctgaaacaa ttcacttttt agaagatttg ggtgtctctg caattgaagt gggtattccc
     7801 ttttcagacc ctgttgcaga tggacctgtt atcgaagaag ctggcttgcg cagtttagcc
     7861 cacgggacct ctacccaggc tttagttgaa accttgaaaa ccattgaaac agagattcca
     7921 ctggtcatca tgacctactt caaccccctc tttcagtacg gtgtggagaa ctttgtcaaa
     7981 gatctggctg atacagctgt gaagggcttg attatcccag acctgcctca tgaacatgcc
     8041 aactttgtag aaccattttt ggcaaataca gatatcgcct tgattccttt agtaagcttg
     8101 accacaggaa ttgagcgcca gaaagagttg attgcagggg cggagggatt cgtctatgcc
     8161 gttgccatca atggggtgac agggaaatca ggcaattacc gtgcagattt ggacaagcac
     8221 ttggcgcaac ttcatcaagt ggccgacatc ccagtcttga caggttttgg tgtatctagt
     8281 caagccgatc tggaacgctt caatgcggtg tcagatggtg ttatcgtcgg ttcaaaaata
     8341 gtaaaagctc tccaccaagg agagccgatt caggatttta tcagacaagc agtagcttac
     8401 caaaaataat caaacaagta gcaagt
//
LOCUS       DAVLWA010000080         8073 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLWA010000080 DAVLWA010000000
VERSION     DAVLWA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.080"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(208..516)
                     /locus_tag="VYN80_001671"
     CDS             complement(208..516)
                     /locus_tag="VYN80_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1541550.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(513..821)
                     /locus_tag="VYN80_001672"
     CDS             complement(513..821)
                     /locus_tag="VYN80_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541551.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(826..2121)
                     /locus_tag="VYN80_001673"
     CDS             complement(826..2121)
                     /locus_tag="VYN80_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1541552.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2139..3548)
                     /locus_tag="VYN80_001674"
     CDS             complement(2139..3548)
                     /locus_tag="VYN80_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1541553.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            3706..4434
                     /locus_tag="VYN80_001675"
     CDS             3706..4434
                     /locus_tag="VYN80_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1541554.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            4893..5087
                     /locus_tag="VYN80_001676"
     CDS             4893..5087
                     /locus_tag="VYN80_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541555.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(5453..6763)
                     /locus_tag="VYN80_001677"
     CDS             complement(5453..6763)
                     /locus_tag="VYN80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1541556.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7000..7872
                     /locus_tag="VYN80_001678"
     CDS             7000..7872
                     /locus_tag="VYN80_001678"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1541557.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            7985..>8073
                     /locus_tag="VYN80_001679"
     CDS             7985..>8073
                     /locus_tag="VYN80_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1541558.1"
                     /translation="MWYNLLMAYSIDFRKKVLSYCERTGSITEA"
BASE COUNT         2495 a         1425 c         1541 g         2612 t
ORIGIN      
        1 aattggagct taacatctat ttccagcaat tttttagaaa ctgcagtgga ctattctaaa
       61 ttcaacacat tataaaaact agagtaaaag aaaaggattg gatcttgtgt aatgcaggat
      121 ccaatccttt caatcatttt gtccaacttt tggaggttcc tacaatgtag ccgtcattaa
      181 taaagacaga tgggaatgac agtgttccta tttattttga tagagatcga tgaattcttt
      241 agatagcaac tgaataatct ctgttgaagc catttggtct tctgcatgca taaatagcaa
      301 ggagaatcct attttttctc cagtagcttc tttttgtatg agattagagt gaatcttgtg
      361 cgcttctact aaggagtctt ccgcttcttc aactttaatt ttcgcttctt ttaaatttcc
      421 tgccttagct agttggatgg cttcaataaa ggatgatttg gctgctccac tattggcaat
      481 gagctgaaaa cagatatatt ccatttcttc tgtcatctta tttctcctat ccatgcaagt
      541 gcttgttcca gaacttttgc tccattcatc attccgtaat cccgcatatc aatggtatct
      601 acagggatat ttcctgcaat ttctttcaca gcaagtaact cataacgaat ttgtggccca
      661 attagaatga catctgcttc atggatattc tttttagctt ctgtcattga ttttgcttgg
      721 atagaaattt caatcccacg ttcagtcgca ctttgttgca tttttttaac aagcatactt
      781 gtcgacattc ccgcattaca tactaataaa atttgtttca taatcttaac cttccatttc
      841 ttgttcaaca actttgtcat taactttgat aaatggaatg tatagaagaa ctccaagtgc
      901 aaagatgatg aattgaacta gaactgctct cacgtcccct gctgttgcta accatgcatt
      961 taagaatact ggtgtagtcc aaggaacttg tataaatgca ggactcatga atcctgtaac
     1021 tgttgctaag tagctgatta aaataccaag gactggaact gtgataaatg gaatagctaa
     1081 tgaaatgtta taaacgattg ggtaaccgaa taatactggt tcattgatat tgaagatacc
     1141 aggtccaaaa gataatttag ccacgttttt agagacagca ttgcgactca ctaagaatgt
     1201 tgctattaat aaacataatg tagatccact accacccatt aaagcgaatg tttgtatttg
     1261 tgataggttg atgatgtgtg gaatggcttg tccattattt gctgcagtga tgttttcagt
     1321 aatgttaatt aatagtaatg gttctaggat ggcactgtaa ataactgctt ggtgaatacc
     1381 aaatagccat aacatatttc ctaaagagta aataataatg accccgatta agcttgtacc
     1441 aatatgacga attggttctt gaataaagat tgtaatgatt gagattaagt tcattccagt
     1501 tatattgaat aataatgctg aaacaacccc aaataaggag atgacggtca tgactggaag
     1561 taatacgcta aatgatctac taacagctgg tggaatattt tcaccaaggt tcatttgtaa
     1621 agctttaacg tttgataatt caatgaataa ttctgttgca ataatcgcta cgataacccc
     1681 ggcgaacatt gcgcctgtac ctgtgttgtt gaatgaaaga acacctgaaa tgtttaccgc
     1741 atcttttgct ccgtcaggaa ctacagaaac tgtatttggc atcatcacaa ttaaagaaac
     1801 taatgatagc attgatgctg ctaacgggtt ttcgaaatct ctgtttttag ctaagaaata
     1861 accaaccatt acagcaataa tcatacctga aatacttaaa gtaccgtttg caattgttat
     1921 tccccaatat tggaatcttg ttaatgtatc cccttggaaa atccacttaa ataccgtgtt
     1981 gttcaaaaga acgattaaac ctgccaaaat atataatggc attactgtta cgaatgcatc
     2041 tcttagggtt tttaaatgaa tttggttccc tagtttacca gcaaaggtgg caaattgatc
     2101 tataaatttt gagtttttca tctgaatatc tcctctcttt actcctcaat ttctacttca
     2161 aaaccgttat tgtcacttac tttgcgatac catcttcctg atctcttgat ggttctcttt
     2221 tgcgtttcta aatcaacgga gataaatccg taacgattct tatatgcatt attccaagac
     2281 cagcaatcaa atgcggtcca agcgtggtat ccaaaacagt gacttccctc ttcaatggct
     2341 ttatgtagcc atcttaaatg ttcttcataa aattcaatac gatacacgtc atcgataact
     2401 ccattttcac cgataaaccg tgcttcgttt tcaacaccca ttccgttttc actgataaac
     2461 catggaatat taccatattc tttcttcaca atcatagcaa tatcataaat ggctttcgga
     2521 aaaatttccc aaccacgata tggattcatg cgacgctccg gccattcata ctctttgaag
     2581 tattggtctg gcatccaggg cgtctgatat tcctcaggat ttgcttgtgc ttgaacacgt
     2641 tttggatggt agtagtttac tccaagaaaa tcaaccgtat ttgatttcat cagttgaagc
     2701 tctttttcgg tatgactcca taacacgcca tctctctcta gctgttttac caatctttct
     2761 gggaaagttc ctttaacagc tggattcaag aagactttgt taaagaagtc atctgtaaat
     2821 cgacttgctt ctaagtcttc tggagaatta cttcttggat aagcaggtgt caagtttaaa
     2881 ataatcccaa tctttccatc aagtcctaat gagcgatata gttgaatcac ttttgcacta
     2941 gcaaggttta gattatagat gacttgtacg gcctcttttc cctttccttt tagatttgga
     3001 taatggaaag catataagta tcctgcttct ggaatgacca ttggctcatt gaaagttgtc
     3061 cagtaatgaa ccttatctcc aaagcatgtg aaagcagtct tggcaaactt cacgaataac
     3121 tccactacat gtttgctttc ccaaccaccg tatttttgaa gaagttccac tggtaaatca
     3181 aaatgatgta aattcatcac aagatccatc tggttctttt tagcttcttc aatgatggca
     3241 ttgtagaaag caataccttt tggatcaggc tcacctgtct ctaaattctt gatgagtcga
     3301 ctccattgga tggaagtgcg gaaagaatta actccaattt ccttcatcaa gtggaaatct
     3361 tcggtgtaag tatgaaaaaa gttactggct acaagaggtc cgacattatc gaaaaaatct
     3421 tctggatgcg ttttgaacca gtagtccatc acattttcat gtacttttcc atattgacct
     3481 tctgtctgag gtccgctaga agcagctccc caccaaaaca gattaggaaa tactaacttt
     3541 tttttcatgc tcaacctcct ttctattatt ctatacataa agtatagcat aattttaaaa
     3601 tttgtctaga catttttaaa aattgttgat acatatcttg tgtttgcctc atttcttgaa
     3661 ctttgttata ctactattag aatattatag agagaaccag attaaatgag tacaaaatat
     3721 ttatttattt acaatgagat tcgtgaaaag attctttgta ataaatatac catgaacgaa
     3781 caattgcctg atgaaatgac attagctaaa cagtttgcct gtagtcgaat gacgatcaaa
     3841 aaagctttag acttgttagt ttctgagggc ttaattttta gaaaacgtgg gcagggaacc
     3901 tttgttctct ctcgtggcag ctcaaaaaga aaattaatcg ttccagaaag agatatccgg
     3961 ggattgacaa aaatatctga agatgctcat tctacaattg actcgaggat tattcacttc
     4021 aaattagaat ttgcaaatga atttttagca gaaaaactac aggtcgcttt gcagagtcca
     4081 gtttataata tttaccgcct gcgtattatt gacggtaaac cttatgttct ggaacaaact
     4141 tatatgagta ccgatgttat tccaggtatt actgaagata ttttacaaaa atcgatttac
     4201 aattacattg aaggaaagtt aggattgcat attgccagtg ctacaaaaat cttgcgagct
     4261 tcttctagtt cagaaaatga gcaacattac ttacagctcc ttccaacgga accggtattt
     4321 gaagtagaac aagtggctta tttggataac ggaactccgt ttgagtactc gattagtcgt
     4381 catcgctatg atttatttga atttaattct tttgcattac gacattcctc ctaggagaaa
     4441 atgtgaaaat gaagccaatc ttttacagac tctagtttaa gaaaaattta aaacagggca
     4501 agaaggtccc atctatgctt aaatggtttc tcttttctaa ataagatggc tttaaaagag
     4561 tgatcgttgt atccatcatg ttgaaaaata tcttcgtata gcttatagag taggtactga
     4621 aattgttcac ctgatctact tcttatagtt atttagtttt aaatagtgtt tcaaacattc
     4681 ttacactgac gagaagtttt tgagtctttt cttgtaacac atatagtata ctgtggttag
     4741 aatagtagac tgtgacttct aacaaattgc tagaaatgaa tttcaatctc ccaatttatt
     4801 tgttcatatc ttcttttaat atattaaata aattctaaat cataatcatt taaaaaaatt
     4861 ttatttttta tttttcatta cgaataatat agatgaaggg gaaagagtat gaaaacagaa
     4921 ctgtttcttt tgctattagt tcaaaaggag aaaaaatgaa agtagaaaat atttcgtata
     4981 gggtggatca tcgtaaattg tttgataata tttcttttga tacttcgagt tcagacgtga
     5041 cattaattac tggtaaaaat ggtacaggaa agtcaacttt actatagtag attgaaacta
     5101 gaatagtaca catctacttc taaaatattg ttagaaatcg atttgactat cctgatctat
     5161 ttgtcctgtt cttatttcat ttcactatat ctcaaattga gtatgacgaa gtgcgctccc
     5221 atgtcctggg aacgcacttt cttcatattt ttcatattct tgaatccatc gataaagact
     5281 attgggatga atttttaaag ttgaactaat catttttaca ggatgagatt tacagcagag
     5341 agtttgaagg ctttatcaaa ggtttttctt ggcataatga cttttcctcg tttccactta
     5401 attttgtgtc tactttatta taccaagtcc actcttaagt tagataataa atctaactta
     5461 aggaagctag aaggatgaga atccaggtgg tcaagagtcc caaacttaag ctgatgggga
     5521 cacccagaat aatttgcttt ttgaaggcaa ggccacgttc ctctatattg ggaagtgaga
     5581 gttgaatgag agaaccagct gatgaaaagg gtgagatatt agtagataga gcgccaataa
     5641 cggtggctgt tgtgagtaag tgaatatcaa tctgaggatt ttgagcactg atgatagcaa
     5701 tgatgggaaa gagggctgga gctacaacgg atagggtgga actaaagagt gacatcactc
     5761 cggctatcac acaaaagaac agaggtaacc agaaatgagg aatggttgtt gtcatgaggt
     5821 gccctatcag tgtgactaaa cctgacttga ccgctagaga cattagtaag ctcatgccgc
     5881 agagcatgat aattgtagcc cagggaacct tagctaaaat ggcttcttgc ttccctaatt
     5941 tgagccttaa ggcgaggcag accatgagta ttgagacaaa gccaatatca aatgtttttt
     6001 gataagtagc tatccaggcg atgtttggga aaatgagatg caacaaggga aaaagccaaa
     6061 ccaaaaccat gctgctgatc atgagcaagg tggtttgtct ttgaaccttg ctgaggagtg
     6121 gtggttggtc aatagtcaag gatgagtttg ttcttccctt actatagtga ctgtaacagg
     6181 ataataaaag caagacgatg agtgggtaga taatgctgac gataaagata tgattgccaa
     6241 gtgaaaaagc ttgctcttcc catcccattt gcttaaacag gccttgaaag acaatgcctg
     6301 agctactggt tatcaaatta gcccctcctg aagctcccca attgacggct tgagctccaa
     6361 tcaaagggtg tttgtccgct ttttgacaga gggtaatcgc tagaggacag caaacggcca
     6421 tagtagtgaa aaatccagca cctaaagcag acaaaagggt tgccatcagg tataaaatca
     6481 tgtagagggc gttagggtgg gtgcgtgtgc ggtagagaat gtgttgagcc aaaacatcaa
     6541 gagtaccgtt agttgttgca acgttataaa agagagagac gctaaaaatg gtaaaaaaga
     6601 gtgaggttgg ccaaaaatga agaagttctt tggggcttaa tcccatgaga gtggttgcga
     6661 tgaggtaaga aaaagcaata gccagcaggc caatattgat tttggtgcgg taaccaattc
     6721 caatggctag agcaatggcg ctaatcatta ttaaatgaat cattgaattg tcctttcagt
     6781 tagaatatgg aaataggttg gcaattttgg cgctcaaaag accacctgtt tcctattggt
     6841 acttgactat tatacacaaa aaaaatacaa ttgtcttgac aattgtattg acacttgtta
     6901 gaattaaaaa tgaggtattc aggcttgtga tttcctatgg aagttaatag taattgcctc
     6961 tagtgcttac aagtgatatt aaaaatagag gacttagtga tgtcaatcat ttcaactgat
     7021 ttaacccctt ttcaaataga tgatacattg aaagcagcct tgcgagaaga tgttcattcc
     7081 gaagattaca gtaccaatgc catttttgat catcatggcc aagccaaggt gtcgcttttt
     7141 gccaaggaag ctggtgtttt agcgggacta accgtttttc aaagggtttt taccctattt
     7201 gatgccgagg tgaccttcca gaatcctcat caatttaagg atggggatcg tttgactagt
     7261 ggcgatttgg ttttagaaat cataggctcg gtgagaagtc tcttaacatg tgaacgcgtt
     7321 gccttgaatt ttttacaaca tttatcaggg attgcttcga tgacagctgc ttatgtagaa
     7381 gccttaggcg atgattgcat taaggtattt gatactcgaa aaactactcc taatttacgt
     7441 ctttttgaga aatatgccgt gagagttggc ggtggctata atcatcgctt taatttatca
     7501 gatgctatca tgctaaaaga caatcacatt gcggcagtag gtagtgttca gaaggcaatt
     7561 gctcaagcgc gtgcctatgc cccttttgtg aaaatggtcg aggtggaagt ggaaagcctt
     7621 gctgctgccg aagaagctgc ggcggcgggt gctgatatta tcatgttgga taatatgtca
     7681 ttggaacaga ttgaacaggc cattacccta attgcaggac gttctcggat tgaatgttct
     7741 ggaaatattg atatgaccac tattagccgt tttcgtggtt tagcgattga ttacgtctcc
     7801 agtggtagtt taacccatag tgctaagagt cttgattttt ccatgaaggg tttaacctac
     7861 cttgatgtct aagttgtaaa ataaactaac tttttaaagg atgtctttcc tctagaacga
     7921 gttttatgtc agatagttta aacgcctctt caaatatagt aaaatgaacc aaaaatagta
     7981 cacaatgtgg tataatcttc ttatggcata ttcaatagat tttcgtaaaa aagttctctc
     8041 ttattgtgag cgaacaggta gtataacaga agc
//
LOCUS       DAVLWA010000081         8024 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLWA010000081 DAVLWA010000000
VERSION     DAVLWA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.081"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            145..309
                     /locus_tag="VYN80_001680"
     CDS             145..309
                     /locus_tag="VYN80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1541559.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            393..959
                     /gene="amaP"
                     /locus_tag="VYN80_001681"
     CDS             393..959
                     /gene="amaP"
                     /locus_tag="VYN80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1541560.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            971..1141
                     /locus_tag="VYN80_001682"
     CDS             971..1141
                     /locus_tag="VYN80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1541561.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1180..1788
                     /locus_tag="VYN80_001683"
     CDS             1180..1788
                     /locus_tag="VYN80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1541562.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1819..2022
                     /locus_tag="VYN80_001684"
     CDS             1819..2022
                     /locus_tag="VYN80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1541563.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2133..2405)
                     /locus_tag="VYN80_001685"
                     /pseudo
     CDS             complement(2133..2405)
                     /locus_tag="VYN80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2714..3001
                     /locus_tag="VYN80_001686"
     CDS             2714..3001
                     /locus_tag="VYN80_001686"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1541564.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3279..4049)
                     /locus_tag="VYN80_001687"
     CDS             complement(3279..4049)
                     /locus_tag="VYN80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1541565.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4064..4690)
                     /locus_tag="VYN80_001688"
                     /pseudo
     CDS             complement(4064..4690)
                     /locus_tag="VYN80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(4596..5198)
                     /locus_tag="VYN80_001689"
     CDS             complement(4596..5198)
                     /locus_tag="VYN80_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1541566.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSTGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            complement(5208..5525)
                     /locus_tag="VYN80_001690"
     CDS             complement(5208..5525)
                     /locus_tag="VYN80_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1541567.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5542..5859)
                     /locus_tag="VYN80_001691"
     CDS             complement(5542..5859)
                     /locus_tag="VYN80_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1541568.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(5874..6770)
                     /locus_tag="VYN80_001692"
     CDS             complement(5874..6770)
                     /locus_tag="VYN80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1541569.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(6773..>8024)
                     /locus_tag="VYN80_001693"
     CDS             complement(6773..>8024)
                     /locus_tag="VYN80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1541570.1"
                     /translation="LQEKLLPVAARLGNNKALVSIRDGMTLTIPLLLIGSLLMVIASF
                     PIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGIVSLS
                     SFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMPDGVP
                     PAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVIGLL
                     ILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFMDNFV
                     FIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVLNIL
                     LLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQIVL
                     IVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         2766 a         1584 c         1269 g         2405 t
ORIGIN      
        1 tattaaagtt atataatagt attaatcaaa gaaattgatt ttttgatatt aaaataaaaa
       61 aggagggtag gcagtgttgt gatcaattat tgctggaggt cttattggtc tcttggcagg
      121 taaaatcact aaaaaagtag ttctatggga atcatcgcaa atgtattcgc tggtttagtc
      181 ggggcatatg caggacaatc tcttttaggt agttggggtc catcaatcgc tgaaatggct
      241 ttgctcccat ctattgtagg tgcagcgatt gtgattactg tagtgtcatt ctttacaggt
      301 agaaagtaaa cttttcgcca gtaaagttag caaactattt ttaaatcaat gacgggaaaa
      361 atagtttaaa tgttaaatcg aaaggattgt atatgtcaaa agcaaagaaa atatgtttca
      421 ttattttctg tattttaatc ttgacaattt tccttcctgt tttgatagat tatcatcaag
      481 ttagtgatct aggtattcat ctacttagct ggagacagaa ctccgtagtt gaattctatc
      541 ttgctagata tgtcttttgg gggacagtgg ttctatcaac tttagtttta ttatccattt
      601 tagttgtgat gttttatcct aaacgttact tggaaatcca acttgaaact aaaaacgata
      661 cattaaaatt aaagaattcg gcaatcgaag gttttgttag aagtttggtg agtgatcata
      721 gattgatcaa gaacccaact gttcatgtaa atttacgaaa aaataaatgt ttcgttcatg
      781 tagaaggtaa aattcttcct tcagacaaca tcgctgacag atgccaaata attcaaaatg
      841 aaataactaa tggattgaag cagttttttg gtattgagcg tcaagtaaaa cttgaagttg
      901 cagtaaaaaa ttaccaacca aaacctcaaa acaaaaagac tgttagtcgt gtgaagtaag
      961 gaagtaaaaa atggaatggc ttaaacaata tcgatatcca attatcgctg gtctcatagg
     1021 cgtatttctg gcttgtttga ttgtctcctt tggcttcttc aaaacaatat ttgtattgat
     1081 tttaggagca ctgggagttg cagctggatt atatatcgaa aaaaactata tagataaata
     1141 aaaaaataaa aattactaat ttaattaaag gagtttcata tgtcaaacga aaaaaacaca
     1201 aacactaacg tagaaaagaa agatgctact gttgtagctc acgaaatcaa aggggaactt
     1261 acttacgaag ataaagttat ccaaaaaatc attggtcttt cactagaaaa cgtttcaggt
     1321 cttttgggaa tcgatggtgg tttcttctca aatcttaaag aaaaaatcgt taacagcgat
     1381 gacgtaacaa gtggtgttaa cgtagaagtt ggtaaaacac aagttgcagt tgacttaaac
     1441 gttattgttg agtaccaaaa aaatgttcca gctttatatt cagaaatcag agaaatcgta
     1501 tcttcagaag ttgctaaaat gactgacttg gaaattgttg aaatcaacgt aaacgttgtc
     1561 gacatcaaaa ctaaagaaca gcatgaagca gactcagtaa gccttcaaga tcgcgtatct
     1621 gacgttgctg aatcaacagg agaattcact tcagaacaat tcgaaaaagc taaatctggt
     1681 cttggatctg gtttctcaac tgttcaagaa aaagttagcg aaggtgtaga agctgttaaa
     1741 ggtgcagcaa atggtgtagt atctcacgaa aacactcgtg taaactaaga taaaataaat
     1801 ataacaggag aaattatcat gtcagtagaa gaaaaattaa atcaagctaa aggttctatt
     1861 aaagaaggtg ttgggaaagc catcggtgat gaaaaaatgg aaaaagaagg agcagctgaa
     1921 aaagttgttt ctaaagtaaa agaagttgcc gaagacgcta aagacgctgt agaaggtgct
     1981 gtagaaggtg ttaaaaacat gttgagtggc gacgataaat aaggttaaaa gttactttat
     2041 cttttttggt aacattagtc aaaagagtct gagtcaagat gattctcaga aaacaaaaag
     2101 ctagagattc ccaattgcgg aactctagct ttttaatttt gcctctttct cttattatat
     2161 ttcagcaggt tgttggccat gagtatgaat cccatgtcaa ttctcacttg acgcttacct
     2221 ctcagatgac atctcttata acccaaacaa acctttatct gcccaaagac agatttcata
     2281 tcaatcttac gtttagcgaa aatttgtcta cccttggaag ataaaagtgc ctgatattct
     2341 ttagttttta aacactggta acgttcattc atatacagtc ccttttgagg ggctgattca
     2401 ggttcataat cgcagtcaac attgatttca aggctgtttg ctttctatct cccctttttc
     2461 ataatgtata gtaaaatgaa ataataacag gacgaatcga tcgggacagt caaatcgatt
     2521 tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact atattttcgt
     2581 acaggtgctt caatagtttc tatagtaaaa tgaaccaaaa atagtacacg ttgcggtata
     2641 atgttcttgt ggcatattca ttagattttc gtaaaaagtt ctctcctatt tgtgagcgaa
     2701 caggtatagt caattgattg tcagtttgaa gaccaagagt cgtaaggcaa aggatatggc
     2761 agaatccatc caagggtggc tggcacaatt tctagtgaac ctctttaaat ctattacgtt
     2821 tgactgtgga aaagagtttt caaaatggaa agatatttcc aatcatcacg atagtgagag
     2881 cttttttgcg aatctaggat gtccaagaca gcggtgtctc aacgaacatt caaatagact
     2941 tttaaggtgt catgacttgc caaagcagac agatttcaac gaagtcagtc aagaatttta
     3001 ggctgggtaa aaactcaatt tcaggagaaa atgaaataaa tattcccaca ataaaacgca
     3061 tagtatcaaa gtttttccaa cacctgatat tatacatttt ttaattttaa agactttttc
     3121 ccagcctcaa gaaatcacat cccacctaaa tcctttggat accaaatacc tcatcaggta
     3181 ttttctgagt tggtaagact gtctagctta atttgacaaa tggagggcgt taaatccagt
     3241 ttttacagga agtctattta aatattttca cactgacctt acaaaatatg aaataaatta
     3301 tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct tacccaattt
     3361 tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga agcaagaaaa
     3421 ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac aattgtagcc
     3481 aaatgagaca gctgggaaaa gaaaataatt gcaagagaat acatcgttgc ttcttttatc
     3541 gaaatagaaa aaaacaccat caataaagat acattgagcg gaccaccacc aatccctaaa
     3601 aaactagaaa tcaagcccaa tagaagacca caaatcatat agaatcctat cttatcaaat
     3661 tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtaacatcac aaatagtaaa
     3721 atcatttgaa caatagatac tagccgtact gataattgag ataacaaaac ttgaaaaata
     3781 agataaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat caatctccat
     3841 tttatctctt tactttgtga aaatcttttg attgttgaaa taatcgccat tataaaaaca
     3901 gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact atccataagt
     3961 ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact agctattata
     4021 atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct ttcaaacatc
     4081 taaaacctag atggctacta gtggaagtag aaatacatcc atttctagca gctactcggt
     4141 atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca tactcctcat
     4201 cttgtatccc atattttggt aactcatagt catcttcatt aaaactagct aataaggtat
     4261 atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga ccaaaatcat
     4321 taggtaaaaa ttcatagaca ggagccgttc ctataaatcc atctaaggca gtattttcat
     4381 aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa agagtatctc
     4441 cccaaagata ttcagattgt cttcctcctc tagcagcata ttcccactgt gcttcagtcg
     4501 gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca acatgaacta
     4561 ctggatggtc ttctaaaccc actagattac tgttctcacc gtagggatgt ttccagcaag
     4621 cgttcggaac ttgtaaccac cacggagccc cagcaggatg aggatatccc tccctttctg
     4681 cctctggcat taatatggtt tcctggaatc acattcccaa tagttaccat cagattttta
     4741 ccatcaaatg ctagattatc aattccttgt aagatctgat ctaatttcac atctcctaca
     4801 tttgccaaag ggaaactctt tgtaactgta cttccaatcg aaatgacaaa cgaagcaagt
     4861 gataccaaac cagtagaaac tgtatcaacc ttgtaaatct tagcgatatt cactcccaag
     4921 caatagatga acaacaagga aacaattggt atacttccct tgaataccaa attattgatg
     4981 tcaacaagcc actgaaaggt tttcgtaata ctttctaggt gaaattgttg tggtaaatcc
     5041 actagaaaag catttaataa caaagcaatg gaacctgtca taataacagg catagtcccc
     5101 acaaatgaat cacgtaaagc aactaaaaac ttctgattcc caactttcat tgcaattgga
     5161 gaaagttttt cttgcatttt atccaacatt gtattcatac caaactccta atttttttca
     5221 aatcgttcgt atacttctac cacttctttc gccaagtcaa caaaagtcag actcgtcatt
     5281 aaatgatctt gcgcgtgtac catcaagagg ctaattgatg ttttttcccc ctgtgcctct
     5341 cttgtcaaca tttctgtttg agccttatgc gcctgtaata aggcagcatt cgcatcagct
     5401 aatcttcgat tggctttaga gaaatcccct ttttttgctg cctgaatagc ctccatagca
     5461 ttacttttgg cttccccacc atacataatt agccccatta cagattctaa attactttca
     5521 tccataacat tctcctcaat cttagtctaa taaactttca gctaaatcta aaactttaga
     5581 accattcatc attccgtaat ctgtcatcgg aataaccgcc acaggaatct gtctatcttt
     5641 tagtttttct tgaaagtccc ctagtaaata gcgaacttga ggacctaaaa gcaacacatt
     5701 tacttctttt gttgctacaa tttcttctgc ttcaggagct ggaactgcaa aaatttctgc
     5761 atccaacccc ttatcttctg ctgccttttg catctttgtc actagcagac ttgtactcat
     5821 acctgctgca catgctaaca taattgttac tttagccata gcttactcct ttatcaccta
     5881 tttactgacc tagccaagcg agatactgta tgtctcactc ctctaattgc cttgctcaat
     5941 gcataaatat tatcgactgt tgccagtccc ccatatcctg cgtcacctat atgttgaata
     6001 tcaactccac aaattttatt tctaagtgca atttccttga tagtatctgt atcagatgtt
     6061 tcttggctag taccaatagc actcaataca agaccaccct tactatgaac gagatcaacg
     6121 acttcacgca actcttggtc atgaaaggct ggaacggttc caactgctgg aacaagtatc
     6181 acatccgcac ctgcttccaa caattgctct gcgacagaaa gctctgccac aggctcattc
     6241 actcctgccc cgtgcatttt accagctata atcaaacctg agaaattctc tttagcagat
     6301 tgaactgctt tgataatctc ttgattgcta actcctgttc caggatttcc agtcaaacag
     6361 acaaagtcaa aacctaactc ctctattcga ttcaatgttt caacactagc aacacgacct
     6421 gcaacaattt cctgtgtttc ctctagcatc tttgcagatg ggtcaatcgg ttccaaattt
     6481 acaccaattg gacaagcaac aagctggtgt aagcgatgaa taacatcatc acctgaacta
     6541 tccaaagcat aaattttagg ctcaaaaaca tcaacacaat tcaataaaat catatcagct
     6601 ccaaaagctc tagcaatttc agaattagtt atatccccaa caaatgtctc acgagtaact
     6661 acattttcag atagaacaac tctaccttca cttgctaaaa tactttgttt taattcttca
     6721 gcattcatct gtaatatctc agatggattt gcactaatta aacgtttcat tctcaatcct
     6781 ccaataactt aaaccgtttt tcaatagcta ttacaaatgg taaataaagt aagacatcca
     6841 aaacaatcaa tacaatttgt aataatgaac cagaaatact tcctgtagca agtaaaccac
     6901 ttataacagg tggcatagtc caacctggat ctgtataagt tagcggaacc aaacctgatg
     6961 ccattgctcc ccacgctact agtaagttaa acattggagc taagataaat ggaactagta
     7021 acaaaatgtt caaaacaata ggaacgccaa acattgctgg ttcatttata ttaaataatc
     7081 caggtataac agttataggt gccaaagttt ttagttgttt actagctttc ttcttatgtg
     7141 ctaaatatcc taacgcaaga actaaaccta tggttgcacc accgccacct atgaatacaa
     7201 aattatccat aaatacgcta gtaaaaatat ttggaagagt ttctccagtt tgataagcta
     7261 ctttatttgc atctagatta gctaaccata acggtttcat aactgcatta acaacatttc
     7321 ctccgtgtaa tcctacaaac caaaaactac tattaaggaa aattaagata agaagaccaa
     7381 taacattatt tcctaaaagt ccaagtggac ctcccaaagc tacttgagca atttcatgaa
     7441 gattaggtaa acttaattta tctaaagttg cataaacaat caaccaacca acaatagtca
     7501 ctgcaccagg aataatggca ctaaagcttt tagatactgc tggtggaaca ccatccggca
     7561 tttttatctg tatattatga tttataaacc attggtagat atagccatta atcaatccta
     7621 aaatcatagc aacaaataaa ccttttgatg ccatgaaagc ggtaggcatt cctgctccag
     7681 cctctcctct aatgaaaggc gtaactgtaa taaaagatga taatgacaca ataccagcag
     7741 gaataccatc aactttgtat tgtctagcca taaagtaagc gataccaaaa ctagctacaa
     7801 gaccaagtaa tccaaaacta ctatcaacac ccttccataa atagtcagca acaccaatat
     7861 cacccagata tttttcccat cctggaatag gaaaacttgc aataaccatt agtaatgaac
     7921 ctatcaacaa taaaggtata gttagagtca taccgtctcg aatagaaacc aacgctttat
     7981 tattacccaa tctagccgct actggaagta acttttcttg taat
//
LOCUS       DAVLWA010000082         7953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLWA010000082 DAVLWA010000000
VERSION     DAVLWA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.082"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..555
                     /locus_tag="VYN80_001694"
     CDS             <1..555
                     /locus_tag="VYN80_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1541571.1"
                     /translation="KFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHTMV
                     QRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGAGI
                     AGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQLR
                     SEEQALHFVPNRNITVVSNFSRTD"
     gene            612..1520
                     /locus_tag="VYN80_001695"
     CDS             612..1520
                     /locus_tag="VYN80_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1541572.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            1472..2062
                     /locus_tag="VYN80_001696"
     CDS             1472..2062
                     /locus_tag="VYN80_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1541573.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            2065..3171
                     /gene="yqeH"
                     /locus_tag="VYN80_001697"
     CDS             2065..3171
                     /gene="yqeH"
                     /locus_tag="VYN80_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1541574.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAYEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            3385..3696
                     /gene="yhbY"
                     /locus_tag="VYN80_001698"
     CDS             3385..3696
                     /gene="yhbY"
                     /locus_tag="VYN80_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1541575.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            3748..4377
                     /locus_tag="VYN80_001699"
     CDS             3748..4377
                     /locus_tag="VYN80_001699"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1541576.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLVPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            4377..4970
                     /gene="yqeK"
                     /locus_tag="VYN80_001700"
     CDS             4377..4970
                     /gene="yqeK"
                     /locus_tag="VYN80_001700"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1541577.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPGLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREVASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            4971..5471
                     /locus_tag="VYN80_001701"
     CDS             4971..5471
                     /locus_tag="VYN80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1541578.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKIAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            5484..5837
                     /gene="rsfS"
                     /locus_tag="VYN80_001702"
     CDS             5484..5837
                     /gene="rsfS"
                     /locus_tag="VYN80_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1541579.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            5940..6686
                     /locus_tag="VYN80_001703"
     CDS             5940..6686
                     /locus_tag="VYN80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1541580.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            6696..7793
                     /locus_tag="VYN80_001704"
     CDS             6696..7793
                     /locus_tag="VYN80_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1541581.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARESDLPEGIHVLGFTEKGRQHLKYLKGQVSLVSRIGKEPWDV
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
BASE COUNT         2374 a         1578 c         1828 g         2173 t
ORIGIN      
        1 aaatttattc aggcttatat tgaaaagcta gatgtgacaa ccattatcga gaatattcta
       61 accaaggtca tttctctttt actgcttttg attgtatttt atattgctaa aaaaatgctt
      121 cataccatgg tgcagagaat tgtcaaacct tctctaaaaa tgtctcgtca tgatgtcgga
      181 cgccaaaaaa ccatctcacg tttactagaa aatgtgttta attatacgct atatttcttt
      241 ttactctact gcattttgtc gattttaggt ttgccagttt ctagtttgct ggctggagct
      301 ggtattgctg gggtagcgat tggtatggga gcccaaggct ttctgtctga tgtcatcaat
      361 ggctttttca tcctctttga acgtcaactg gatgtgggag atgaggtcgt tctgacaaat
      421 ggaccgatta ctgtatcggg taaggttgtc agtgtgggaa ttcgtacgac acagcttcgt
      481 agcgaggagc aagcccttca ctttgtccct aaccgaaata tcacagttgt tagcaatttc
      541 tcacgcacag actagacctg ttattttaag taatttgtgg tacaatagag ggagtttaat
      601 aaggagaaaa gatggtttta gaaaagcagt tgggcaatgg ttgtacctgg atagacctag
      661 acctaggaaa gttgaataaa ctagaagacc tttctgaaat ttacggtttg gacaaggaaa
      721 ccattgaata cgcactggat agaaacgagc gcgcccacat ggactaccac cgtgaaagtg
      781 ggacggttac ctttatctat aatgtcctag atgtaaaaaa ggacaaggcc tactatgaga
      841 cttttcccat gacctttatt gtcgagcatc gtcgcctgat taccattagt aataccaaga
      901 acgcctatgt cattgaacag atgactcgtt atctggagag ccatgacacg ctttcgattt
      961 ataagtttct ctttgccagt ctggaaatta tcagcaatgc ctactatcct gtcattgagc
     1021 agatggacaa gagtagggat gaggtcaatg acctcttgcg ccagcgaact accaagaaaa
     1081 acctctttgt cctgtctgat ttggagactg gtatggttta tctgacggca gctgccaaac
     1141 aaaatcggat tttgttagag catattcaag gtcatgcctt gtatcgtagt tttgatgaga
     1201 ttgagagaga acagtttgat gatgccatga ttgaggctca tcagctggta tccatgacag
     1261 acctaatctc tcagatttta cagcagcttt cagcctctta caacaatatt ctaaacaata
     1321 atctgaatga caatttgaca accttgacta tcatttcagt cttgctagct gttttggcag
     1381 tcgtgacagg ctttttcgga atgaatgttc ccttaccttt aacagatgag ccccatgctt
     1441 ggctctatat cagtttggct agtgcaggtt tgtggattgt tttatccttg ttactaagga
     1501 aaattgcgaa aaaaagttaa gaaaaggagc cagaatggcg attgaaaatt atataccaga
     1561 ttttgctgtg gaagcagtct atgatctgac agtcccaagc ctgcaggcgc agggaatcaa
     1621 ggctgttttg gtcgatttgg ataataccct cattgcttgg aacaaccctg atggaacgcc
     1681 agagatgaag caatggctac atgaccttcg ggacgcgggt attggcatta tcgtagtgtc
     1741 aaataacacc aaaaaacgcg ttcaacgagc agttgagaaa tttgggattg attacgttta
     1801 ctgggccttg aagcccttca catttggtat tgaccgtgcc atgaaggaat tccactatga
     1861 caaaaaggaa gtagtcatgg ttggcgacca gctcatgaca gatatacgag cagcccaccg
     1921 tgcagggatt cggtcaattt tagtcaaacc cttggtccaa catgactcaa tcaaaacgca
     1981 gattaaccga actcgtgagc gtcgtgttat gagaaaaatc actgaaaagt acggaccgat
     2041 tacatataaa aaaggaattt aactatggaa gaaattctct gtattggttg tggagcaacc
     2101 attcagacga cagataaagc tggtcttggt tttacccccc agtcggcact tgaaaaaggt
     2161 ttggagactg gcgaagtcta ttgccaacgc tgtttccgtc tccgccacta caatgaaatc
     2221 acagatgtcc agttgacgaa cgatgatttc ctcaagctct tgcacgaggt gggagacagt
     2281 gatgctttag tggtcaatgt cattgatatc tttgatttta atggatctgt catcccaggt
     2341 ttaccacgtt tcgtctcggg caatgatgtc ctcttggtag gaaataaaaa agatatcctt
     2401 cctaagtcag ttaagtctgg taagattagc cagtggctca tgaaacgtgc ctatgaagaa
     2461 ggtcttcgtc cagtcgatgt ggtcctaact tcagcacaaa ataaacatgc cattaaggaa
     2521 gtcattgaca agattgaaca ctaccgtaag ggccgcgatg tctatgtggt cggtgtgacc
     2581 aacgttggaa aatcaactct aatcaatgct attatccaag aaatcacggg tgatcagaat
     2641 gtcatcacta cttcacgctt cccagggaca accttggaca aaatagagat tccgcttgac
     2701 gacggatctt atatttacga tacgccggga attatccacc gccaccagat ggctcactac
     2761 ttgacggcca aaaacctcaa gtatgtcagt cctaaaaagg aaatcaagcc taagacctat
     2821 cagcttaatc ctgagcaaac cctattttta ggtggtttgg gacgctttga ctttatagca
     2881 ggagaaaagc aaggatttac tgctttcttt gataatgaac tcaaactcca tcgtagcaag
     2941 cttgaaggag ctagtgcttt ctacgataag cacctgggaa ctcttctgac accaccaaat
     3001 agcaaggaaa aagaagattt cccaaggcta gtccagcatg tctttaccat taaagataag
     3061 acagacctag tcatctcagg cctaggctgg attcgtgtaa caggcacagc aaaagtcgcc
     3121 gtctgggcac cagaaggcgt cgccgtcgtt acacgaaaag caattattta aaacaaaatc
     3181 gctgagcgaa caaagtgagc tcatatagcg atagttattg ccgtggtgtg attgccaatc
     3241 tgtagattgg caaggggcac aattgagacc aaggctcaat tgtgaggtca ggaaatccat
     3301 ttttcaaaga tgaaatcttt ggaaaattag ttccgaccgt ccctcaccac ctaaaataac
     3361 tatcaaaaaa caaggaagaa aattatgtca ttaacatcaa aacaacgtgc cttcctcaac
     3421 agccaggcac acaccctcaa acctatcatc caaatcggga aaaatggact caacgaccaa
     3481 atcaaaacca gcgtccgtca agctcttgat gcgcgtgaat taatcaaggt tactctctta
     3541 caaaacacag atgaaaacat ccacgaagta gctgaaattt tggaagaaga aatcggtgtg
     3601 gatacagtcc aaaaaatagg acgcatcttg attttgttta aacaatctag caagaaagaa
     3661 aatcgcaaga tttctaagaa agtcaaagaa atctaagacc gaaactccaa ataactgttt
     3721 ttatagagaa ataaagggga ctagcctatg acaatcgaac tattgactcc ctttaccaag
     3781 gtagagttgg agccagaaat caaggagaaa aaacgcaaac aagttgggat tttagggggg
     3841 aattttaacc ctgttcacaa tgcccatctc attgttgcgg atcaagtacg gcaacagttg
     3901 ggactggatc aagttcttct cgtgcctgaa taccaacctc ctcacgttga taaaaaggaa
     3961 accatccctg aacaccatcg tctcaagatg cttgagttgg caattgaggg gattgacggc
     4021 ctagtcattg aaaccattga gttggagcgc aagggtattt cctacaccta cgacaccatg
     4081 aaaatcttga cagagcaaca tccagatacg gattattact ttatcatcgg cgccgacatg
     4141 gttgactatc tgcctaagtg gtaccgaatt gatgaactgg ttgacatggt tcagtttgtg
     4201 ggggttcagc gtccacgcta caaggcaggg acttcctatc cagttatctg ggtggacgta
     4261 ccgctcatgg atatctcgtc cagcatggtg cgggacttca ttgcccaagg tcggaaaccc
     4321 aactttctcc tacctcagcc agtgctagac tacatcgaga aggaggggct ctactgatgt
     4381 cctatcaaga ctatatcaac tgctcccgtg aggccttgtt ggaaaaaatg gcagagcttc
     4441 tacctgagaa acgtctaacc cattgtctag gtgtggagcg tgccgctatg gaattagctc
     4501 agcgctttgg tgtagatgtt gagaaagcaa gtctagcagg tcttcttcat gactatgcta
     4561 aaaagttgtc agatcaggaa tttctagact tgattgaccg ttaccagcta gaccctggcc
     4621 tcaaaaactg gggcaataat gtctggcatg gtatggtcgg aatttacaaa attcaggaag
     4681 atttggattt gcatgattca gaaatcctgc gagccattga agtccataca gtcggtgctg
     4741 gtcagatgac agacctagat aaggttgtct atgtagcaga ctatatcgag cacaatcgat
     4801 cctttcctgg agtagatgtg gcgcgtgagg ttgcaagtct atcgctcaat aaggcggtgg
     4861 cctacgaaac agctcgtacc gtggagtatc tagctcatca gggattcccc atctatcccc
     4921 aaacccttga aacctataac gcctttgtgc actatttgaa agagaactaa atgaagatgg
     4981 cttttataat cattgatgtt cagaatgttt tagtagaaac tgggttccag acaaaaagtc
     5041 tattggaaaa aatttcttat ttacaaaacc aggctagaag caagaatatt gaaattatct
     5101 atgttcaaca tattgagaac tctgaagctc aaacatcaga agattggcag ttatctgcgc
     5161 ttttaaatcg aaaacctgct gaaaaggtct ttcagaagaa atataacagt attttcaaag
     5221 aaactggctt aaaagaatac ttggataagc aggggattga aaaattagtt ttatgtggta
     5281 tgcagacaga atattgtgtg gatacctctg tcaagattgc ctttgaatat ggctatcagc
     5341 ttattgttcc agaaggtgct gtcacaacct ttgatgggga tgacattcca gcagaaacga
     5401 ttaatgaatt ttatgaggac atttgggagg agcgctttgc agatgtccta gattacaaac
     5461 atattttcta aaaagaggat taaatgaacg aaaaagaatt actagaacta gtcgtgaaag
     5521 cggctgatga gaaacgtgcg gaggatatcc tcgcacttga tgtacaagat ttgactagtg
     5581 tgacggacta ctttgtcatc actagctcaa tgaatagccg tcagctggac gctatcgcag
     5641 ctaatatccg tgaaaaagta gctcaagcag gctttaaagg tagccatgtc gaaggcgatg
     5701 cagctggagg ctgggtctta ctggacctcg gtgctgttgt cgtgcatatc ttttcagaag
     5761 aaatgcgtgc ccattataat ctagagaagc tatggcatga ggcgaattca gtagatattt
     5821 cagaagctct tgcttagaag atgaactcag ttgtagtcaa ctgggtttct ttgcttattt
     5881 tagaaaaatt gatagaaagg taaagggcta gtggtgttta ccatggagct cttagtatca
     5941 tgattatggc gacttatgaa acctttgcgg ctgtctatga tgcggtcatg gacgatagtt
     6001 tatacgacaa atggacgaat ttttctctgc gtcatttgcc taagaccaag gagagaaaga
     6061 aactcttgga attggcttgt gggacaggaa ttcaatcagt gcgcttctct caggctggtt
     6121 ttgatgtgac tggacttgac ttgagtgcgg atatgttgaa gattgcggag aagagagcaa
     6181 cttcagccaa gcaaaagatt gcttttatag aaggtaatat gctaaatttg tccaaggcag
     6241 ggaaatacga ttttgtcacg tgttattcgg actctatctg ctatatgcag gatgaggtgg
     6301 aagtagggga cgtctttaag gacgtgtaca atgcgctgaa tgaagaggga gttttcatct
     6361 ttgacgtgca ctcgacctac cagacagatg aagtattccc tggctattcc taccatgaaa
     6421 atgcggaaga ttttgccatg ctttgggata catatgagga cgaagttcct cactccatcg
     6481 tgcatgagct gactttcttt atcaaggagg ctgacggttc ctttagtcgc cacgatgaag
     6541 tacatgagga gaggacctat gagatcttga cctatgatat cttgctggaa caggctggat
     6601 tcaagtcctt caaactctat gcagactttg aggataagga gccaacagaa actagcaccc
     6661 gttggttttt tgtggcgcag aagtaggaga ttagcatgac catcacaggt attatcgcgg
     6721 agttcaatcc ttttcataat ggccataaat acctgctgga tcaggcagag ggactgaaaa
     6781 ttgttgccat gtcagggaat ttcatgcagc gtggagagcc tgctattgtg gataagtgga
     6841 caagggccca gatggcgctg gaaaatggcg cagacttggt agtggaattg ccctttttag
     6901 tcagtgttca ggcagctgat ttctttggtc aaggagctgt ggatatcttg gatcgcttgg
     6961 gcattgatag tctagttttt gggacagagg aagttcgaga ttatcagaaa atcgctgact
     7021 tatacacaga gaaaggtgct gagatggaga aatttgtgga aaatctgcct gattcccttt
     7081 cctatcccca gaaaacccaa gctatgtgga aggaatttgc tggtcttgat ttttcaggta
     7141 atacccccaa tcatgttctt gctctggcct atgcaaaggc ggttgcagga cgaaatatca
     7201 agctgcatcc gattcagcgt cagggggcag gttaccattc tgtgaataaa gatgtggact
     7261 ttgcctcggc gacagccctc cgtcaacatc agaaggacca agatttctta gaacgtttta
     7321 tgccttctgt tgccctattt gagcaggcca gtaaggtgat ctgggaagac tattttccct
     7381 tgctccgcta tcaaatcttg tcaaatcccg acctaaccac catctatcag gtcaatcaag
     7441 aaatggctgt gcgcattaaa gaagctatta aaacagccca gtctgtggaa gaattggtcg
     7501 agttagtgac caccaaacgt tacaccaagg cgcgtgtcag acgcctcttg acctatatct
     7561 tggtgcaggc tagagaaagt gacttgccag aagggattca tgtccttggc tttaccgaaa
     7621 aaggcaggca acatcttaaa tatctgaaag ggcaggtcag tctagtcagc agaattggca
     7681 aggaaccttg ggatgtcatg actcaaaagg cagaccagat ttaccaattg ggaaatccaa
     7741 gtatagcaga acagaatttt ggcagagtgc cgattagaat agaaacaaac taagtctact
     7801 gaaaggtagg cttttttatt gtagaaattt tagctaacaa attgaagata aagtttttgg
     7861 aaaatgataa actccctaat acagaagaca aaaagtcaat aaagaaaatt agtatatgaa
     7921 ccttaatctt tatctttgaa tccgctttat gct
//
LOCUS       DAVLWA010000083         7651 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLWA010000083 DAVLWA010000000
VERSION     DAVLWA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.083"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(720..1940)
                     /gene="divIB"
                     /locus_tag="VYN80_001705"
     CDS             complement(720..1940)
                     /gene="divIB"
                     /locus_tag="VYN80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1541582.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYISVLFNDSEQIKAFT
                     SELAQINPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            complement(1950..3008)
                     /locus_tag="VYN80_001706"
     CDS             complement(1950..3008)
                     /locus_tag="VYN80_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1541583.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQALFSKGG
                     FVSVPPVIAACVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(3010..4362)
                     /locus_tag="VYN80_001707"
     CDS             complement(3010..4362)
                     /locus_tag="VYN80_001707"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1541584.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(4466..5107)
                     /locus_tag="VYN80_001708"
     CDS             complement(4466..5107)
                     /locus_tag="VYN80_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541585.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLDLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWGQVDQVIRVTDLSHR"
     gene            complement(5109..7130)
                     /locus_tag="VYN80_001709"
     CDS             complement(5109..7130)
                     /locus_tag="VYN80_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1541586.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASFFILRSRDWVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(7201..7500)
                     /locus_tag="VYN80_001710"
                     /pseudo
     CDS             complement(7201..7500)
                     /locus_tag="VYN80_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2288 a         1671 c         1314 g         2378 t
ORIGIN      
        1 ttcttgacct attagttgct ctaaccattt ttttcctgtt tcccaactaa tacatgtttc
       61 ttactggatg gtatatatag atgatactgt ccctcataag gagtaggtag tatcttaatc
      121 ttatcaacaa ttctcataac aaacctaacc tttaataaat aaaagagggg ctaagtaaaa
      181 catactctag tccctcagtc cgcaatcaat aaaataatga ctaattctat taatctcttg
      241 ctgcaagact aatacggaaa attcaaatga ctgtctaaaa tgaaattgaa aaagcttgat
      301 attagtaagt gaacaaactt caacaaccag caagagcaag gattgctaaa cctaatccga
      361 tacctgttgc aagtccagct gaaaaatcac taactggtac tgaaatttct tcaatttcct
      421 caaataaaaa gttatcttgc atgattatca ctccctatct ttttaacaac aggctactcc
      481 aattaagaga actaccccag ttccaagtaa aactcccccc caaaattcac catctgtagg
      541 ggctacgatt tcttcaattt cttcaaataa gaatacttca tctgacatat gtgttacctt
      601 cttcataaaa aatttttttg taatcgatta cattgcaaat ataacataaa gaaaaacaga
      661 tgtcaaatat taaacgtaaa aacatggcaa ctaaagagct ataggagaaa aggtaaaacc
      721 tagcgacgcg atgagcgctg ggtcgtttgg tttcgattgc tctcttcctc ttgttttttc
      781 tgttcttctt ctaaacgttt acgttcctct tcctgccttt tctcagcttc ttcggcctct
      841 tttttagctt tttcttctgc ttccataatc aatttatccg ctacagtgta gctgtagact
      901 ccagcttcca tatcgaccac acttggttct gacaattgag gcttaatctt actgtaataa
      961 ggcaatttct tactcatttc agaaagcgga accaagactt cgtccgaatc attcatagtc
     1021 aatcgaatta aatcggaagt caccttgctt ggggctaatt ccaccttttg gatagccacc
     1081 ttgagttctg ggttaatttg agcaagttct gacgtaaagg ctttgatttg ttcgctgtca
     1141 ttaaagagaa ctgatatata agtttctggt aaactgttca gactcacaga actagtctca
     1201 agctgaccac tggaaagaat aggataatga ttttcaccag aaatatagta ggccacaata
     1261 tcatattcct tgaccttaat agtgaactta gttggaaatt gatagacaag ttgagctgat
     1321 tcaacccaat agttagactt aatctgcttt tcatattttg ccttgtctag cagaaggtta
     1381 atcgtataat ccgaatcctg aatgcctgaa gcctgtcgaa tatcatcagc tgtagtttgc
     1441 accgttccct caacacgaat atctttcatg gtcgcataag gactgagcaa gtaggcagag
     1501 acaatcaata aaagcagact tggaaataaa atcgtgaagg ctcgcaagat atggatacca
     1561 ggaatctttg ctttggctgg tttttccttt gtagcctttt tagcaagctt tttatcctgt
     1621 tcctccttct ctttagactc tggttcttct ttctcttctt tctctttgtc agcctctgag
     1681 gatgctactt tttcttcaga ctcttcctta gctgattctg aatcttcctg gtctgtttca
     1741 ctctcctggt cctgtttgtc ctctgacttc tcagattctt ctcccattcg agcttgtctt
     1801 tccttttcct tctcctcagc tagagccgct tcttcttcag ccttcttttt tagatattct
     1861 tggtttcgtt tctgccattc tgataactct ttcaattctt cgaggatttc tttgtcctca
     1921 tttttcttat cttttgacat ttactttcct tatgataaat cttttttcaa caattgataa
     1981 aaatctgcta gagatttcaa ttccttagaa gctttcatct tagcttggta atcttccttg
     2041 tgacttagta agtgagaaag cttctcttcc aaactatcca aggtcaaatc gctttcttga
     2101 aggtcttctg catagccttt cttaacaaag taagctgcat tttcaatctg gtcaccacga
     2161 ctagcttcac gaccaagtgg cacaatgaca tgcaattttg ctatcgccaa gagctcaaaa
     2221 atcgtattgg caccaccacg tgtcacaaca atatcagcca attccatcaa gggttgatag
     2281 agatcggtca catagtcaac acgaaaaaga ttttggctca actcattcag gctagaatct
     2341 ccagttagat tgataatatt gtagcgctct gttagttctt tcttatggtc tgtcaccaat
     2401 tggttaaaga cacgagcacc tgcagaaccg ccaacaaaca atacagtcgg caatttatga
     2461 ttaaagtggc tttgaatatc caccaattca tctggttctg gatttttttg atccgaaacc
     2521 ttggtcactg ctcccacatg ctcaacctta gacaaactcg aagcttgctc aaaggttgaa
     2581 tacatcttag tcgcaaattt ataggcgatt ttattggcca agcccataga caggtcagat
     2641 tcgtgaataa agacaggcac tcctgacaca cacgcagcga taacaggcgg tactgagaca
     2701 aatcctccct ttgaaaaaag agcctgtgga cgcagtcgca acatgataaa gagcgattgg
     2761 acaattcccc aaccaacttt gaagacatcc agcatatttt gccaagagaa atagcgacgc
     2821 aattttccag tcgcaataga atggaaggtg acatccaaac ctgacttaag gatttcttgg
     2881 tgttcgatac cacgcttgtc cccgatatag tggacttccc aaccatcttc gatgaactta
     2941 ggcattaaca aaagattgag ggtaacgtgt ccaaccgtcc ccccacctgt aaagacaatt
     3001 tttttcatat tattctttta actccgctac tgtgtcgata aagaggtcgc cacgtacttc
     3061 aaagttagca tacatatccc agctggcatt ggcaggacta agaagaacca catctccttg
     3121 agtcgcaagt tcataggcct tgcgggtcgc atctgcaata tctgtcgcct ccacataagc
     3181 gacaccagcc ttgtctgctg cccgtttgac acgttctgca gattgaccca ggatgaccat
     3241 cttcttgagt ccagtaatgt ctggcaccaa ttcgtcaaac tcattgccac ggtccaaacc
     3301 acctgcaatc aagacgacct tgctgttgtc aaatcctgac aaggcttttt gagtagccaa
     3361 gatattagtt gatttactgt cgttatagaa tttaacaccc ttgatgtcat ccacaaactg
     3421 gagacggtgt ttgacaccac cgaaggctga aagagtttcc ttgatggttt gattgtccac
     3481 accacgaagc ttggctacag caatagtcgc aagggcattt tccacattgt ggctacctgg
     3541 aacaccgatt tcattcgctg ccatgactac ttcaccacgg aagtagagtt gaccatcttc
     3601 cagataagct ccatcaacct tttcaagtgt tgaaaatggt acaacagtgg cttctgtctt
     3661 ggaagtcaag tcttttgcca agtcttgatt aaagttcaag acaaggaaat cagctgctgt
     3721 catcttgttc tggatattcc acttggctgc tacatattcc gaaaatgacc catggtagtc
     3781 gatatgagtt ggcatgaggt tggtaataac cgcaatctct ggatggaatt cttgaacacc
     3841 catgagttgg aaagaagaaa gttccataac aagcgtgtcc ttatctgatg ctatttgagc
     3901 aacctgactg gcaggatagc cgatattccc tgataaaaga ccatgttgcc cagcagcagt
     3961 caaaacttcc ccaatcatag tcgttgtggt tgtcttaccg ttcgatcctg tgataccaat
     4021 aatcggtgct tctgaaatca aataagccaa ttccacctca gtcaagactg gaattccctt
     4081 ggccaaagcc ttttcaatca tgggattgct gtaggggata cctggatttt ttaccatgag
     4141 ggcaaactct tcatccaaga gttccaaagg atggccacct gtaatgacct tgatcccttc
     4201 ttccagcaaa cattgggcag ctggattgtc ctcgaatggt ttcccatcat ttactgtcac
     4261 aatggcacct aacttgtcca acaaacgagc cgcagattca ccagacttgg ccaaacctaa
     4321 aacaagaact ttcttatttt taaattgatc tattactttc atgtctcgaa ctccatttct
     4381 actcctacta ttttaccatt tttatggaaa taaaaaagcc acaaagtgtg tttgtgactc
     4441 tttcttctaa ctgaatctta ccatatcatc tatgtgataa atcggtaact cgaatgacct
     4501 gatccacttg cccccaaatc agaggattgt gggtcgcaat aataatggtc cgattcggat
     4561 tttttaaaga ttctaggatg gaaagtaatt cctcagagtt tttagggtct aaggaagcgg
     4621 ttggttcatc tgcgaggatc aaaggtggat cctttaaaat tatcttcgct agtgcaacac
     4681 gttgtgcttc tcctcctgat aactcaaata taggttgctt caaatccaaa taagagaggt
     4741 ttacacgatt tagagcttgt ttcatcaaag agattttctc tttttccttc aactttttac
     4801 caactaaacc cagatcgaga ttctctttga cagtttggct ttcaattaag ccaaaatctt
     4861 gaaataaata tcctaagtaa tctctaaaga aaacagaagg cttgatgtcc ttaagagaag
     4921 taccatcata gatgatttgc cctttgtcat atggctctaa tcgtccaatc atattcaaga
     4981 gtgttgtctt accacagcca cttgtaccga ttaaggcata aattttccca ccttcaaaat
     5041 gaagattcgt atctgaaaat agctgacggc ttccaaattt tttagatata ttctttagtt
     5101 caatcatcct attttccttt cataattgtc atagaaacac gagattcttt ctgtgcttga
     5161 cggtaaagcg tcaaaactgc actagctaga aagactaata aagtgagtaa gccaattacc
     5221 cagtctctac tgcgtaaaat aaagaaacta gcaccaaata caaaactggc aaattggcta
     5281 accatatact gagcatgtgt ttcaaaaaat cgtaaacctg aaattcgttt aatcaaaata
     5341 tctcggcgga attgctcgaa atatagaaga ttgacagaat aaaatagtaa caaggaacta
     5401 gctatcccca caatagctcc taagattaaa gttgctgttt cagtttgaac ttcattataa
     5461 cgagttagat aaacacttct tccttcttta agataggata cttgctcata aattccagct
     5521 ttcttcaaga gttctagccc actctcatat cctttgataa agagttgttt tccagcattg
     5581 atagaccaac tagataagga tataaaacta tcacctgtag aagtcggcgt gaataccact
     5641 aaaatcggat cagtcaaata ctgagtggat acgggattct caccgttatt ataaacaaac
     5701 cgcttttctc ccattgaaag ataactaacg tgcgctttca tctcataatc caaaggagca
     5761 cttgcctcct caccagattt tccataataa ctcaatcttt cttcaaaaac tttcttaagt
     5821 tctgcttctc gagagcgcaa atgttctggg agcaagagga taaactcacc tttttggaga
     5881 tgggctagct tctgtttggt ctcagcatct accacgacct tttccttgtc caaataactg
     5941 ggactaacat agagcgtatt agcatctgga ctataggtat ccagtgtctc tccctgttca
     6001 ttttttcctt gtggattggc aaaatggagc agattatcct ttacataaag agcttgttct
     6061 tcttcgattg cttccttggc aaaggcatac cacttgctct gattttctgt atcttttcct
     6121 ctatcaccta agccaaaaga aatttgataa tagtctgctc tatcctgcca tgcttgtttt
     6181 gaaatttcaa gttctttcaa tcgttggtaa gacgtcaaac ctgtcttaac agcgtagcct
     6241 actgtaaaaa cagctactaa ctgacacaat agggttaaaa tcatcaggcg tttaaggggt
     6301 aatctcccct taataacggg aactaatgct ttgtaattca aactcattag ataaagaagc
     6361 attagtaaaa ttgaaatcgc caataaaaac aacagataga aactaatccc aaaaccatag
     6421 gtggctaaca agataggata aaacaaacct tgactaaaaa gaacgactcc cccacctagg
     6481 aaggaaagga gggctgatag aaggagccat ttgatatcag tcgataaaga atgccccatg
     6541 atggataaga gagtctgacc agaaaagagt tttatacctg ctgctctcat ttccttaatc
     6601 cgagtgataa tcactaaagc aaagaaagat aagccaaata ttgctaaact aattaaaata
     6661 aggggattta gtaatattcg aaaagcaaga aaatagggcg gtatctttcg gtcagcactt
     6721 gctttataac ccaaatctcc taatttatcg gcaagctttt ctttcgtcaa ggagcctgac
     6781 aaaaggagat aactatttag cggatcacta cgttcacgac tttcttggct agcttcttgg
     6841 aattcttttg gtaaagttcc ctgaccataa gttgcataag taaagtgagt cgtcccatcc
     6901 ttactcggct ctacaattct tctagctatt aaactctgtt ctgagtttgc aaaattctcc
     6961 aattcctgtt caaatacctc acgcgtcggt tcctgagtat cttttttgac acgaagtaaa
     7021 gaaacggaat catagcttgc atataaatat tgtggcgcac gtaagacaat aatccaagca
     7081 aggaagaagc tgagaaaaaa agttgataat aatatgaata gtttcttcat agtagactcc
     7141 ttgtaaacaa aattccccct gtaatttctt acaaggggaa ttatctaaga aaactaaata
     7201 ttagtcatag tcatagtaaa atgctacttg ttctccccat ttagtccaaa tccatgcata
     7261 ggaggttttt ccagcaggag cttctccttt atcagaatga ccatcccatc tgctcacgat
     7321 ggatgaataa tgatattttt taccatgata gtaatttgaa aaagcctaac cacctcctga
     7381 accttctcca tatgtccata ctcctccatc tggatattgt acagcagctg atgcagctcc
     7441 caataatgta aaatttgaaa taagagctag agcaagtaat ctatgttttt tcgttttcat
     7501 tttatttttt ctttcaaaaa aatcacacct tgagcaacaa tgcaacaaaa taaatcctcc
     7561 tctctctttt gttgaaatcg ctttcttatg tgataagaat aacattttta ttatttgttg
     7621 tcaaggaaaa aatcgaattt tttagatgtt t
//
LOCUS       DAVLWA010000084         7578 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLWA010000084 DAVLWA010000000
VERSION     DAVLWA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.084"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(513..1373)
                     /locus_tag="VYN80_001711"
     CDS             complement(513..1373)
                     /locus_tag="VYN80_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1541587.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(1648..2634)
                     /locus_tag="VYN80_001712"
     CDS             complement(1648..2634)
                     /locus_tag="VYN80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1541588.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(2906..4381)
                     /locus_tag="VYN80_001713"
     CDS             complement(2906..4381)
                     /locus_tag="VYN80_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1541589.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(4639..5562)
                     /locus_tag="VYN80_001714"
     CDS             complement(4639..5562)
                     /locus_tag="VYN80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1541590.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(5576..6505)
                     /locus_tag="VYN80_001715"
     CDS             complement(5576..6505)
                     /locus_tag="VYN80_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1541591.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(6824..7045)
                     /locus_tag="VYN80_001716"
     CDS             complement(6824..7045)
                     /locus_tag="VYN80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541592.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(6999..7259)
                     /locus_tag="VYN80_001717"
     CDS             complement(6999..7259)
                     /locus_tag="VYN80_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541593.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            7339..7476
                     /locus_tag="VYN80_001718"
                     /pseudo
     CDS             7339..7476
                     /locus_tag="VYN80_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2268 a         1508 c         1434 g         2368 t
ORIGIN      
        1 agtatcgtaa gagttcaatc tgatttttgt taaacatata gtaagtatta ttaactttag
       61 ctaataatcg tcctgagcct aaagcatcat atttttcaag atagcacaaa tattttgatt
      121 catccatcct aaattatctc cacatcaaat tctgtgcatt ctcgtttgac atcagcggac
      181 tcctcctata aaattacgat tagattgcat atatctagtg attgtcgagt taatgaaatt
      241 attgtatact atattcaaaa tattgtcaac aaaaaaatag aaaattttct gataatttgt
      301 gattaaaacc aataccatac ctctcccatc attgaaaagc gcagtcatta cttaaaaagt
      361 cactcgttta tgttataata ggctccataa tatccatagg ggatttaccc actacaaata
      421 ttatagagcc gattttcatt gagtagcaat cctgtcatcc ttggcaggat ttttctattt
      481 caaaaaagca agtcgattga cctgcttctg ctttactttt cttgtgctaa tgttttaaaa
      541 tctttgtcta atatgggttg cacttctatt tgattggcgt ctagttggtg gtaaaaagat
      601 gggggtaata cctctaagaa ttttgcatag tccaagcgag ctatggattc atagtcttct
      661 ggtttagagc cgtctcctgt gatttgaacc aagtcaatct cccactggac ttccttatcc
      721 accaattgct caatataggc tgcaggaaca aacggttctc tcggcaaaac tgtcttgact
      781 ccttgagcta gatggtaaac gccatgcact tttccttctc catcctgata gaaggaaact
      841 ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa ctgagccaag
      901 ccatctgcct ctaaaaagct tttcaagttc tcacgactaa agaaaatagg attaaaaatt
      961 ccttggcaaa tcgtaaaacg agctacaggg tctgctagat aggcttgaat acttgcttgg
     1021 aaataagctt caaaatcaaa accatctagc ccttcaatat cagtccctgt ataagcaagc
     1081 aaagcatcta aaaatgactt gataatctgc cagtctgtct ttgttagtag gtcaggaaga
     1141 tcgacacggt aagccttttg accatcagca taactgacct taaagagaaa ttgtgattgc
     1201 cccactatcg cacactcaat atactcgaga cgattaagag gctgacggag atagactaca
     1261 tcatagctat gtgactccaa accgtctagc aaggccaaga tcgacttggc attcaaaatc
     1321 tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc cataactgga
     1381 ctcctttgaa atcgtttaca tacagtatac cttatttttc tgaaagatac aaaaacaaac
     1441 tttatattct tcgaaaatct cttcaaaccg cgtcagcttc gccttgccgt agatatatgt
     1501 aactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcag cctgcggcta
     1561 gcttcctagt ttgctctttg attttcattg agtattagat ttttgagtac aaagcataga
     1621 aaaacagccc ctaaggactg tttgatctta tacagtagct gcctgatcca agctttcacc
     1681 gatagcggct aggcgctcaa tcacttcagc ttgtgtcaat tcattttttg aaacatagcg
     1741 gttacgtggg tgaacacggc actcgtgtga gcatccacga aggtacttgt cttcattttc
     1801 ttctgatgtc aagatacgac ggttacagaa gggatttcca cagttaacat aacgttcaca
     1861 tggtgttcca tcaaaccagt ctttccctac gatggttggg ttgacatggt tgacatcaac
     1921 ggcaatacgc tcgtcaaaga cgtacatttt cccatcccaa agctcacctt gaacttctgg
     1981 gtctttaccg taagttgcga ttcctccgtg caattggcca acatctttgt aaccttcacg
     2041 aaccatccag cctgagaatt tctcacagcg aacaccacct gtacagtaaa ccacgacacg
     2101 cttgtccatg aatttttctt tgttgtcacg gacccattgt ggtaactcac ggaagttgcg
     2161 aatatctggg cgaatagccc cacggaaatg tcctaggtcg tactcataat cgttacgtgt
     2221 gtcaaggaca acggtatctt tatcaagaag cgcttctttg aactcttttg gagacaagta
     2281 agcacctgtt gtttcaagtg ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag
     2341 gtggacaatt tctttcttgt agcgaacaaa catcttcttg aaggcttgtt cactttcttc
     2401 gtcaatcttg aaccagagat cttccattcc tggaaggctg tgaacgtagt ccatgtattt
     2461 ttgagttgtt tcatagtcac ctgaaactgt tccgttaatt ccctcgtcag cgactaggat
     2521 acggccttta aggccgattg atttacagaa agccaagtgg tctgcagcaa attgctctgc
     2581 attttcaatt ggagtataaa ggtagtaaag taagacacga atatcttttg ccataagatt
     2641 tgttctcttt tctattctta aattttctaa attttttatt tatccatact agtatacctg
     2701 cttgagaaaa cgaatgcaat ttatttgact ggaaattgta gaggattctc gaaatttcct
     2761 gaatggttaa aataaggaca agagaaaaca tggatatcta tatccttgtg ccaaaaaaac
     2821 cactgccctc cccagaccaa cctgaggaaa gcagtgattc ttattttagg agttaggaat
     2881 gaatacacga aatcaattta gctgattatt ttttgttttt caagaattca tcgtattgtt
     2941 tttgcatttc gttcaatact ttttcgtagg caccttcaga tttcaatttt tccatcaatt
     3001 ctggaatagc tttatctggg tctacagtac cagtgttgat agctgtatca aattgttgca
     3061 ttgtgttagc aatagctgag atttcagatt tcacattgtc agtattgaag ataaatccaa
     3121 gcgctggaga ttctttagct tctgccaatt ctttcttaga attttcgatt tgttggtctg
     3181 taacgttttc gttgatgtaa aggatccagt tgttaccagt gttccatcca cccatgtgag
     3241 tgtttccttt gtagccatca agaacgcgaa cacggttttc tttaccttca attttttccc
     3301 agttcttgcc ttctggaccg taaacaagac cgttcaagag ttctggattc gtattcaaga
     3361 ggttcaagat ttccattgat ttttctttgt tcttagagtt gtttgagatg acaaagttag
     3421 caacttgtgt tgtttggttt ttcttgatga agttagtaat tggtttgatt tggatatctt
     3481 tgttggcaac acgtgaaagc aagctgttac cgtagtcagc tggtcctact gtttcttcac
     3541 gaacgaacca agtatcttgt tgaaggtcaa aggaagtatc gcttgttgcg acgtcttttg
     3601 gaatgtagcc agcttcatag aatttgtgaa gagtcttcaa gtgttctttg aaacgaggca
     3661 cttcgtaacg gtttacaact ttagtagtat cgccttcaag gtcgataacg aatggaagac
     3721 cgtttgctac tgggtagtca aagttatcag atgggatgaa aactttacca atagcaaatg
     3781 gtactacgtc tggagctttt tctttaattt gtttcaagac tggctcaaga gtttcgtaag
     3841 aagtaacacc tgaaatatcg ataccatatt tagcaaggag agttccgttg aaggcaaagt
     3901 tttgagatga tgcaacgttg gctgcaactg gaacagcgta aatcttacca tttacagtat
     3961 tacccttgat gtaagctggg tcaagtgctt tgtaaaggtc tttaccttct tttttgtaca
     4021 attctgtcaa gtcagcgtaa gcaccttttt gagcatttac aatatagtta tctgcaaagg
     4081 caatatcata gttttcacca gatgatgtga taactgacat tttcttacca tagtcacccc
     4141 agccaaggta ttggatatcc aatttggcac caactttttc ttcaatgatt ttgttggcat
     4201 ttgctaacaa ttcatccaag ttgtctggtt tgtcaccgat ttggtacatt ttgataacag
     4261 gtttgtcacc tgaatcagca gcttttttgc tgttacctgt caaatttcca caagcagcaa
     4321 gacctgcagc caaagcgact acactagcag atgcaaaagc atattttttc cagtttttca
     4381 tgataaaaac tccttttttt atttttaaac ttataaacaa tgtaatgatc ttatactcaa
     4441 tgaaaatcaa agagcaaact agaaaactag ccgcaggctg ctcaaagcac tgctttgagg
     4501 ttgtagataa gactgacgaa gtcagttaca tatatctacg gcaaggcgac gttgacgcgg
     4561 tttgaatttg attttcgaag agtattaact tcacacaagg gaagttggga actgagaaat
     4621 gttctttttc aataagcact attctttcac accaccgata gtcaaacctt ttacaaagta
     4681 gcgttggaag aatggatata aaatcgcgat tggaagggtt gcaaccacaa ccatggccat
     4741 acgacctgtt tctttcggta gagcaactcc cagttgacca gtcaagccga ccgctttggc
     4801 aatgtagtcc atattttgtt ggatttgcat gagcaaatat tgcaatggat acaagttatc
     4861 actcttgatg taaagaaggg cgttgaacca gtcattccag aaaccaagag ctgtcaaaag
     4921 cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag atttggaaga aaatccgggc
     4981 ctcactggca ccatcgatac gagccgattc gagaatggct tctggaatgg tcttcttgaa
     5041 gaaggaacgc atcaagatga tattgaatgg tgagagaagc attggaacaa tcaaggccca
     5101 aacagtgtca ccaagctgaa gtacacgggt caccatgata taacctggta ccaaaccagc
     5161 gttgaacaac atactgagaa ggacgaagat ggtaaaaaat ctgcgatact taaaggttgt
     5221 ccgtgaaata gcgtaggcat aggttgttgt gataaagaca tttgtcaatg tcccaactac
     5281 ggttacaaag acagagatga agagggcttg taggatttta tccttaaact gtgccaaaaa
     5341 ctcaaaaccg tctaagccaa attgggatgg gaagaagcta tagccgtatt ggaggaggct
     5401 tttctcgtct gtcactgaaa taatgataac gaatacaaaa ggtaggatac aagagagggc
     5461 aatcaaaccc gaaatgatac tgaagaagat atctgctttc ttactgaagg agtgaatgcc
     5521 gacattatca attttttctt ttttaatttt cttttttgcc atattctcct cctttctaga
     5581 acaaagctga gtttggatcg actcgtcttg caagcaagtt tgataggata accagaatca
     5641 aaccaacaac ggattggtaa agaccggctg ctgcagccat accgatatct gctgtctgag
     5701 tcaaaccatt aaaaacatat acgtccaaaa cgttggttac attgtaaagc tgaccagcat
     5761 tgtgtgggat ttgatagaag agaccgaagt ctgcgcggaa gatatttccg actgcaagga
     5821 tggtcaatac agttacaagc ggagtcaact gaggaatggt tacgttgcga atacgttgcc
     5881 acttgctagc tccgtccact gtcgctgctt cgtagtaggt tggatcaatt cccatgatcg
     5941 tcgcatagta catgacactg ctatatccaa aacctttcca aatacctagg aaaagtagga
     6001 gatagggcca gatgcccagg tcagcgtaga aattgacttc tttgagacca agactttcca
     6061 atagatgatt gaacacccct ttatcaatat ttaggaaggc atctgtaaag aaactgatga
     6121 taacccaaga caagaagtaa gggaacaaca tagaagtttg gaaaatcttc accattctct
     6181 tagaacggag ctcgctgagg ataatggcaa tccctacaga tacaactaaa cctagaaaga
     6241 taaagccaag attgtagagg acagtatttc gtgtgataat aaaggcgtct cttgaactaa
     6301 ataagaatct gaaattatcg agtccgaccc atttactatt tatgatacta tctatgaaac
     6361 cattactggt catgtggtag tctttgaagg caaccacgtt cccaaatact ggaatgtaaa
     6421 agaatagaat caaccagagt gcccctggca aaaccatcaa gagaaagatc cagttgtctc
     6481 tcaatgtttt tgaaaacttt ttcataattt cctccctttt tattttgata tccatctaaa
     6541 aattcttttt tagacttttg ataacgatta cattattagt atactcctat ttgcaggtta
     6601 ggttaaactc ctaattatag aaaaaactcc acaaattatg tagcagattt aaaactttat
     6661 caccactatc aaacaaatgt cctaaatcaa ttgtttattt tatctctatt agcccagtga
     6721 tggcgtcact ctgttataag catccaacaa cggggtatac tgaaaaatct ccagactagg
     6781 gaactcagag atagttccta atctggagat ttttaatatg ttattaggcg tttgctttca
     6841 acttagcaat aacctcttta agattatcaa tcacctctgc ttcagtatgc tcagagcctt
     6901 tttcatctgc caagaacaaa actgcttttt gaagttcttt ttgagagttt tcaaggacat
     6961 ccttatctac tgtttcaagg tttgagtctt taagaagttt acttaattcc ttggctaatt
     7021 tcttgagttt gatttgcaga ctcatcttct cctgctgttt ctttgcccgc tgtttgtcct
     7081 ccatccttag ttgctgactg gctttcctta atggactcta gggaagcaat ggcatctttg
     7141 actgtttgca agatatcacg taaaccttgc tctgtcaaac tatcatctgc aaaagctttc
     7201 ttagcctctg ccaaaaccag acgtgctgaa tctgtggtag gattcgatac acctgtcaat
     7261 gatctcaaaa gattttctaa ggtttgagtc tgcttactaa tactagacta aaatcaaaaa
     7321 gtattatata acagtgatat gaaatcaact aaagaagaaa tccaaaccat caaaacactt
     7381 ttaaaagact ctcgtacagc taaatatcat aaacgccttc aaatcgttct attttgtctg
     7441 atgggcaaat cttataaaga gattatagaa cttttatagt agtttgaaat aagatgtgaa
     7501 catctctatc aggaaagtca aattaattta tagaaatatt ttagcagcca aggtgtactg
     7561 ttatagattc aatacact
//
LOCUS       DAVLWA010000085         7566 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLWA010000085 DAVLWA010000000
VERSION     DAVLWA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.085"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..379
                     /locus_tag="VYN80_001719"
     CDS             <1..379
                     /locus_tag="VYN80_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1541594.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEEMMTSDFFEAWFQK
                     FLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEALLSCSCFN"
     gene            465..1415
                     /locus_tag="VYN80_001720"
     CDS             465..1415
                     /locus_tag="VYN80_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1541595.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPISLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1592..2599
                     /gene="gap"
                     /locus_tag="VYN80_001721"
     CDS             1592..2599
                     /gene="gap"
                     /locus_tag="VYN80_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1541596.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2666..3502)
                     /locus_tag="VYN80_001722"
     CDS             complement(2666..3502)
                     /locus_tag="VYN80_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1541597.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3628..5823
                     /locus_tag="VYN80_001723"
     CDS             3628..5823
                     /locus_tag="VYN80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1541598.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5876..6028
                     /gene="rpmG"
                     /locus_tag="VYN80_001724"
     CDS             5876..6028
                     /gene="rpmG"
                     /locus_tag="VYN80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1541599.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6038..6214
                     /gene="secE"
                     /locus_tag="VYN80_001725"
     CDS             6038..6214
                     /gene="secE"
                     /locus_tag="VYN80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1541600.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6269..6805
                     /gene="nusG"
                     /locus_tag="VYN80_001726"
     CDS             6269..6805
                     /gene="nusG"
                     /locus_tag="VYN80_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1541601.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            6927..7406
                     /locus_tag="VYN80_001727"
     CDS             6927..7406
                     /locus_tag="VYN80_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1541602.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT         2396 a         1294 c         1653 g         2223 t
ORIGIN      
        1 aagaggtaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
       61 tggtgagtta atcgctccaa tgacttacga agagatgatg acgagcgact tttttgaagc
      121 ttggtttcag aagtttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgcaagattc catagaatgg gtaagttaga acttttatgc gaggagtttg ggcataaact
      241 tttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttatgagg ctcttttgtc
      361 ctgctcttgt ttcaattgac tatagttcac ggatacagtt gggaaagaag ctattaaata
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaaagat
      481 gggcaagaaa tggatctgag tcaaacgatg cttcttatgc tcaagtagtg agtctttatg
      541 atgatacaag tatctctgtt agtaataatg agacggacaa agtgttgact ggatccctct
      601 atactgaaac aaatgaacag ggattaacga ttccaatcag tttactaaaa aattggaatg
      661 aacagacagg aaaaaatttg acaactaatg atcttattgg caaatcagtc tcagccagca
      721 ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag attgtacgtg
      781 taatcaatga tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg
      841 aaacgatttt gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca
      901 aagatccatc acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg
      961 tgctttctca acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat
     1021 tattgattgt gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta
     1081 tttacatcaa tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc
     1141 agaatcgtgg tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct
     1201 tgctgctggc atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc
     1261 acttttaccc atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc
     1321 tagttttcgc tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta
     1381 aaatggatcc tgtagaatcg ttacgctatg aataataagg gattccttga tttacagtct
     1441 taaaaagaaa ctcacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt
     1501 ataataggtt tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa
     1561 tttcatgatt ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt
     1621 cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac
     1681 acgcatcaac gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac
     1741 tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt ggattcgaag ttaacggtaa
     1801 attcgttaaa gtttctgctg aacgtgatcc agagcaaatc gactgggcta ctgacggtgt
     1861 agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct
     1921 taaaggtgga gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt
     1981 tgtattcaac actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc
     2041 atgtactaca aactgcttgg ctccaatggc taaagctctt caagataact ttggtgttgt
     2101 tgaaggattg atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc
     2161 acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc
     2221 aactggtgct gctaaagcta tcggtcttgt aatcccagaa ttgaacggta aacttgacgg
     2281 atctgcacaa cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga
     2341 aaagaacgtt actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata
     2401 cggttacaca gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt
     2461 gtttgacgca actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt
     2521 atcatggtac gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt
     2581 cgcaaaaatt gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt
     2641 ttatatagaa aaatggatga cacgatcatc cattcttttt taattctttt tcaaatgtat
     2701 ttgaaagggt agtgaaagtt agcttttcta aagtaagtgg gtgggtaaag gaaagtcgga
     2761 aggcatgaag cataagccga cttgtctttg atttactatt atagagaggg tcgcccagga
     2821 taggaagatt atgatgcgaa aggtgcacac gaatctgatg ggttcgccct gtctttagct
     2881 tgcaacgagc caaggaagtc ttgtttgaga attgctttaa tctgcttaca tacgtttcag
     2941 catattgccc attttttgca tcaactattc tttttctacg atcatggcga tcacgtccaa
     3001 ttttgtctct gaaaacaagt tcttttctgt tgatatttcc atcaactaga gcccaatatt
     3061 ctctagaaat ctcttttttc tccaataagc gattgagaat gggcaggata aaaggatttt
     3121 tggcaaagag aactaagcca ctggtttcca tgtccagacg atgaacgaca tagcaggttt
     3181 ggccaacata ggtactgaca tggttaagaa gggcaatttc gtttggttga ttaccatgcg
     3241 ttttcatccc ctctggtttg tttacaataa tcaagtgttg atcttgataa acttcctgca
     3301 ctaagtctgg gttgccccaa gggatcgtct tttggggata atcttcctcg tcaaaagtca
     3361 actggcaaac atctccagga tttacgattt cgttccagtg gacttcttct tgatttatca
     3421 aaatatgttt cttgattctc aaaaaatgac ggatttttct agggatgagg agttgttcct
     3481 caagtaattg ctttaccgtc atttgaggta tagaggcggg taatgtaaat gtgaattgca
     3541 tacagatatt gtaacaaaaa aagccctatt tggataggaa atagctaaat tcttgtcttc
     3601 ctatgatgaa gatgataaaa taaacgcatg aaattagata aattatttga gaaatttctt
     3661 tctcttttta aaaaagaaac aagtgaacta gaggactctg attctactat cttacgtcgc
     3721 tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga ttcgaaaatt ctggcgtcgt
     3781 tatcatctaa caaagattat ccttatacta ggtttgagtg caggcttgct agttggaatc
     3841 tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt tgcaaaatgc cttgaaaact
     3901 cggactctta tttttgaccg tgaagaaaaa gaggctggtg ccttgtctgg tcaaaaggga
     3961 acctatgttg agctgactga catcagtaaa aacttgcaga atgctgttat tgcgacagaa
     4021 gaccgttctt tctataaaaa tgacgggatt aactatggcc gtttcttctt ggctattgtc
     4081 actgctggac gttcaggtgg tggctctacc attacccaac agctggctaa aaacgcctat
     4141 ttatcgcagg atcaaactgt tgagagaaaa gcgaaagaat ttttccttgc cttagaatta
     4201 agcaaaaaat atagtaagga gcaaattcta accatgtacc ttaacaacgc ttattttgga
     4261 aatggtgtgt ggggtgtaga agatgcgagt aagaaatact ttggagtttc tgcatcagaa
     4321 gtgagtctgg atcaagctgc gactctggca gggatgctca aggggccgga actgtataat
     4381 cccttgaatt ccgtagaaga ttctactaat cggcgcgata ctgtcttgca gaatatggtt
     4441 gcagcaggat atattgataa aaaccaagaa accgaagctg ctgaagttga tatgacttcg
     4501 caattgcacg ataagtatga aggaaaaatc tcagattacc gttatccctc ttattttgat
     4561 gcggtggtta atgaagctgt ttccaagtat aatctaacag aggaagagat tgtcaataat
     4621 ggctaccgca tttacacaga gctggaccaa aactaccaag caaatatgca gattgtttat
     4681 gaaaacacat cgctatttcc gagggcagag gatggaacgt ttgctcaatc aggaagtgta
     4741 gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg gtcaagttgc tgacaatgat
     4801 aaaactggat tccggaattt caactatgca acccaatcaa agcgtagtcc tggttctaca
     4861 attaagcctt tagttgttta tacaccagca gttgaagcag gctgggcttt gaataagcag
     4921 ttggataacc ataccatgca gtatgatagc tataaggttg ataactatgc agggatcaaa
     4981 acaagtcgag aagttcctat gtatcaatcc ttggcagaat cgcttaatct acctgctgtt
     5041 gccactgtta atgatttggg tgttgacaag gcttttgagg caggcgaaaa attcggactc
     5101 aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg gaagcggtgt tgaaaccaac
     5161 cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg aaggtttaat gcctgaagct
     5221 cattttatta gtagaattga aaatgctagt ggacaagtta ttgcgagtca taaaaattca
     5281 caaaaacggg tgattgataa gtctgtagct gacaagatga ccagtatgat gttggggact
     5341 ttcaccaacg gtaccggtat tagttcatcg cctgcagact atgtcatggc agggaaaact
     5401 ggaacaactg aagcagtttt caatccggag tacacaagtg accagtgggt aattggttat
     5461 actccggatg tagtgattag ccactggctt ggctttccga ccactgatga aaatcactat
     5521 ctagctggct ctacttcaaa cggtgcagct catgtcttta gaaacattgc caataccatt
     5581 ttaccttata cgccaggaag tacctttacg gttgaaaatg cttataagca aaatggaatt
     5641 gcaccagcca atacaaaaag acaagtacaa accaatgata atagccagac agatgataat
     5701 ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg aggctagccg ggctatctca
     5761 gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt cgatagtcaa tctatttcgc
     5821 taagatgctt gtcaaagcct agctttcttg ttataatgga taagatggag gcgttatggc
     5881 actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg agaaactatt caatcaagat
     5941 cagcggaaac cccaagccta cacgactaga agtaaataaa ttttgtaagc attgtggcaa
     6001 gtacactaca cacagagaaa cgagatagga gagagcgatg cgttttattg gagatatttt
     6061 tagacttctt aaagacacaa catggccaac tcgcaaggaa agctggagag attttcgttc
     6121 tatcatggaa tacacagctt tctttgtagt aattatttac atttttgacc agttgattgt
     6181 ttcaggtttg attcgattta ttaacatttt ttagaagatt agtggagtta attacactag
     6241 aaatcttcta tttatgaaag gaaatatcat ggatagtttt gataaaggat ggtttgtttt
     6301 acaaacttat tctggttatg aaaataaggt gaaagaaaat ctattacaac gtgcacaaac
     6361 ctacaatatg ttggataata ttctacgcgt tgaaattcca acacaaacag tgcaagttga
     6421 aaaaaatgga aagagaaaag aagtagaaga aaatcgcttt ccaggttatg ttcttgtaga
     6481 aatggtcatg acagatgaag cttggtttgt tgttcgaaac acaccaaatg ttacaggatt
     6541 tgtcggatca cacgggaaca gatcaaaacc aactccatta ttggaacaag aaattcgtga
     6601 cattttggta tctatgggac aaactgttca agaatttgat ttcgatgttg agattggtca
     6661 aactgtacgt attattgatg gtgcttttgc agactacact ggtaagatta cagaaattga
     6721 taataacaaa gtgaaaatga ttatctctat gtttggtaat gacacagttg cagaagtaaa
     6781 cctaaaccaa atcgcagaat tataaccctg agagaggctg gagcctctct tttttgtgca
     6841 gtttaggagc taaagggaac agaatggaga aaatggaaca aatgtgtttt ctaatctgtt
     6901 agactgtatc tagaaagggg aaaattatga ttaaagaatt gtatgaagaa gtccaaggga
     6961 ctgtgtataa gtgtagaaat gaatattacc ttcatttatg ggaattgtcg gattgggagc
     7021 aagaaggcat gctctgctta catgaattga ttagtagaga agaaggactg gtagacgata
     7081 ttccacgttt aaggaaatat ttcaagacca agtttcgaaa tcgaatttta gactatatcc
     7141 gtaaacagga aagtcagaag cgtagatacg ataaagaacc ctatgaagaa gtgggtgaga
     7201 tcagtcatcg tataagtgag gggggtctct ggctagatga ttattatctc tttcatgaaa
     7261 cactaagaga ttatagaaac aaacaaagta aagagaaaca agaagaacta gaacgcgtct
     7321 taagcaatga acgatttcga gggcgtcaaa gagtattaag agacttacgc attgtgttta
     7381 aggagtttac tatccgtacc cactagtaag tcatgcaaaa aaaatgaaaa aaattagaaa
     7441 aagtagttga caaagtttga aaaggctgta taatagtaag agttgaaaat aacaactcag
     7501 gtccgttggt caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc
     7561 cgtacg
//
LOCUS       DAVLWA010000086         7530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLWA010000086 DAVLWA010000000
VERSION     DAVLWA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.086"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            181..528
                     /gene="ssrA"
                     /locus_tag="VYN80_001728"
     tmRNA           181..528
                     /gene="ssrA"
                     /locus_tag="VYN80_001728"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(711..798)
                     /locus_tag="VYN80_001729"
     tRNA            complement(711..798)
                     /locus_tag="VYN80_001729"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(762..764),aa:Ser,seq:gga)
     gene            960..1700
                     /locus_tag="VYN80_001730"
     CDS             960..1700
                     /locus_tag="VYN80_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1541603.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1697..2608
                     /gene="pfkB"
                     /locus_tag="VYN80_001731"
     CDS             1697..2608
                     /gene="pfkB"
                     /locus_tag="VYN80_001731"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1541604.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2605..4557
                     /locus_tag="VYN80_001732"
     CDS             2605..4557
                     /locus_tag="VYN80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1541605.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4721..6946
                     /locus_tag="VYN80_001733"
     CDS             4721..6946
                     /locus_tag="VYN80_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1541606.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7099..7527)
                     /locus_tag="VYN80_001734"
     CDS             complement(7099..7527)
                     /locus_tag="VYN80_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541607.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
BASE COUNT         2143 a         1425 c         1726 g         2236 t
ORIGIN      
        1 gattttatag tttttaaact caaatgaatt gaaataaaga gagtacgaaa attctcatct
       61 gaaagtattt tagaataatt ctcttcgtga atttcttcaa aacagatagc ttcatcttag
      121 gtatgtgatt tctttttgca tttttgagtt agataaggta taatggtttt attgtctttt
      181 ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt gtggcgacgt
      241 aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag ctgcctaaaa
      301 accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt attattagcg
      361 agatacgatt aagccttgtc tagcggcttg ataagagatt gatagactcg cagtttctag
      421 acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt gtagacaaat
      481 atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccattatt cctttgcatt
      541 cttttgcatt ccttggtaaa acgttgttaa atcaacgttt tttattttta tctttggtat
      601 tcctttgtat tcttttgcta aaaagggagt cacaaacaga ccctatttta aaaaaaggat
      661 agaaaaaagg atacaacatt tgtcgcatcc taaaaataat cttttttcga cggaagacat
      721 gggattcgaa cccacgcacg ctattacacg cctaccgcgt ttccaacacg gcctcttaag
      781 cctcttgagt aatcttccaa tacttactca aatagtctac cataaaggct cttatcttgc
      841 aataaaaatt ctagaaataa gaaaaatgat agattttgaa agaaaatgat aaaaaatgct
      901 tgacttcgaa agaaagtatg atagaatgaa tagtgtaaac gataacagga ggtgattcag
      961 tgttaaaaac agaacgtaaa caactaattt tagaggagtt aaatcagcat catgtagttt
     1021 ctctagaaaa attagttagt ttgctagaaa cgtcagaatc aacggttcga agagacttgg
     1081 atgagttgga agcggaaaac aagcttcgtc gtgtgcatgg tggagcagaa ctcccccact
     1141 ccttacagga agaagaaacc attcaagaaa aatctgtcaa aaaccttcaa gaaaagaagt
     1201 tgctggctca gaaagcagcc tctctcatta aagaaaaaga tgtcatcttt atcgatgctg
     1261 gaacaacaac tgcttttttg attcatgaat tggtcaataa gaatgttaca gttgtgacca
     1321 actccattca ccatgccgct cagttggttg aaaagcagat tccaactgtc atggttggag
     1381 gaaacgtcaa gacggcgaca gatgctagta tcgggggcgt tgctcttaac cagattaacc
     1441 aattgcactt tgaccgtgcc tttatcggaa tgaatggtgt tgacgatggc tattatacga
     1501 ctcctgatat ggaggaggga gctgtgaaaa gagctatttt ggagaatgcc aagcagacct
     1561 acgtcttggt ggattcgtca aaaattggac aaacttgctt tgccaaggta gcaccactca
     1621 aacgcgctat cgttatcact agtcaagggc atgagctctt gcaggttatt aaggagaaaa
     1681 cggaggtaat agaagtatga tttatacagt cacactcaat ccatccattg actatatcgt
     1741 tcgtttggac caagtcaaag ttggtagtgt aaatcgtatg gacagtgatg ataagtttgc
     1801 tggtgggaaa ggaatcaatg tcagccgtgt cttgaaacgt ttgaatatac caaatacagc
     1861 gacgggattt atcggtggct ttactggtaa atttatcaca gatactttag cagaggaaga
     1921 aatcgagaca cgttttgtcc aggtggcaga agatactcgt atcaatgtta aaatcaaagc
     1981 agaccaagaa acagaaatca acggaacggg tccaactgtt gaaccggttc agctagaaga
     2041 attgaaagct attttatcta gtctgacagc agaagataca gttgtctttg caggttcaag
     2101 tgctaaaaat ctaggcaatg ttatctataa ggatttgatt tccttgacgc gccagactgg
     2161 tgcgcaagtg gtctgtgact ttgaaggaca gaccttaatt gatagtttgg actaccagcc
     2221 tcttcttgta aaaccaaaca atcatgaact tggagcgatt tttggggtta aactcgaaag
     2281 tttagatgaa attgagaaat acgctcgtga gttactggct aagggtgctc aaaatgttat
     2341 tatctctatg gctggtgatg gcgcccttct tgtcacatct gagggagctt acttcgctaa
     2401 accaatcaaa ggaacagtca aaaattcagt tggagctggt gattctatgg ttgctggatt
     2461 cacaggtgaa tttgtcaaat caaaagacgc agtagaagcc ttcaaatggg gagtggcttg
     2521 cggaacggca actaccttct cagatgactt ggcaacggcg gaatttatta aagaaacata
     2581 tggaaaagtt gaggtagaaa aaagatgaaa attcaagacc tattgagaaa agatgtcatg
     2641 ttgctagatt tgcaggcaac tgaaaaaaca gctgtcatcg acgagatgat taaaaatttg
     2701 acagagcacg gttatgtaac agattttgaa acatttaaag aaggaatttt ggcgcgtgaa
     2761 gctttgactt ctactggttt gggtgatgga atcgcaatgc ctcacagcaa aaacgctgct
     2821 gtcaaagaag cgacagttct atttgctaag tcaaataagg gtgttgacta cgagagcttg
     2881 gatggacaag caactgacct cttcttcatg attgcagctc cagaaggtgc caatgatact
     2941 cacttggcag ccttggcaga attgtctcaa tacttgatga aagacggttt tgcagacaaa
     3001 cttcgtcaag caacatctgc agaccaagtt atcgaacttt ttgaccaagc ttcagaaaaa
     3061 actgaggaac ttgttcaagc acctgctaat gactctggtg actttatcgt agctgttaca
     3121 gcttgtacaa caggtattgc ccacacttac atggcccaag aagcccttca aaaagtagct
     3181 gctgaaatgg gggttggtat caaggtcgaa accaacggtg ctagcggtgt tggaaatcaa
     3241 ctaactgcag aagatatccg taaggctaaa gctattatca ttgcagcaga caaggccgtt
     3301 gaaatggatc gatttgatgg aaaaccattg atcaatcgtc cagttgctga cggtatccgt
     3361 aagacagaag agctaattaa cttggctctt tcaggagata ctgaagtcta ccgtgccgct
     3421 aatggtgcca aagctgcaac agcctctaac gaaaaacaaa gccttggtgg tgccttgtac
     3481 aaacacttga tgagtggtgt atctcaaatg ttaccattcg ttatcggtgg tggtatcatg
     3541 attgcccttg ccttcttgat tgacggtgct ttgggtgttc caaatgaaaa ccttggcaat
     3601 cttggttctt accatgagtt agcttctatg ttcatgaaaa ttggtggagc tgcctttggt
     3661 ttgatgcttc cagtctttgc gggttatgtt gcctactcta ttgctgaaaa accgggtttg
     3721 gtagcaggtt tcgtggctgg tgctattgcc aaagaaggtt ttgcctttgg taaaattcct
     3781 tatgccgcag gtggtgaagc aacttcaact cttgcaggtg tctcatctgg tttcctaggt
     3841 gcccttgttg gtggatttat cgcaggtgcc ttggttcttg ccatcaagaa atacgttaaa
     3901 gttcctcgtt cactcgaagg tgctaaatca atccttctat tgccacttct tggaacaatc
     3961 ttgacaggat ttgttatgct agctgtgaat atcccaatgg ctgcaatcaa cactgctatg
     4021 aatgacttcc taggcggtct tggaggaggt tcagctgtcc ttcttggtat cgtccttggt
     4081 ggaatgatgg ctgttgacat gggtggacca gttaataaag cagcttatgt ctttggtaca
     4141 ggtacgcttg cagcaactgt ttcttcaggt ggttctgtag ccatggcagc agttatggct
     4201 ggaggaatgg tgccaccact tgcaatcttt gtcgcaactc ttcttttcaa agataaattt
     4261 actaaggaag aacgtaactc tggtttgaca aacatcatca tgggcttgtc atttatcact
     4321 gagggagcga ttccatttgg tgccgctgac ccagctcgtg cgattccaag cttcatcctt
     4381 ggttcagcag tagcaggtgg actcgttggt cttactggta tcaaactcat ggcgccacac
     4441 ggaggaatct tcgttatcgc ccttacttca aatgctctcc tttacctcgt ttctgtcttg
     4501 gtaggagcaa tcgtaagtgg tgtggtttat ggttacctac gcaaaccaca agcataaaaa
     4561 atagaaaaat gaaaagattg gaccgtttgg tgcagtcttt ttctcttccc gaaatgcctg
     4621 tgaaatatgg tataatagaa gaatggcaaa caagaataca agtacaacaa gacggagacc
     4681 gtctaaagca gaactggaaa gaaaagaagc gattcaacga atgttgattt cgttaggaat
     4741 tgcgatttta ttgattttcg cagccttcaa attaggggct gcaggtataa ccctttataa
     4801 tttaattcgc ttgctagtgg gtagcctagc ttatctggcg atattcggcc tattaatcta
     4861 tctcttcttt ttcaagtgga tacgaaaaca ggaaggactc ttatctggct ttttcaccat
     4921 atttgctggc ttactcttga tttttgaggc ctacttggtt tggaaatatg gtttggacaa
     4981 gtccgttcta aaagggacca tggctcaggt tgtgacagat ctgactggtt ttcgaacgac
     5041 tagctttgct ggagggggct tgatcggggt cgctctttat attccaacag cctttctctt
     5101 ttcaaatatc ggaacttact ttattggttc tatcttgatt ttagtgggtt ctctcctagt
     5161 cagcccttgg tctgtttacg atattgctga atttttcagt agaggctttg ccaaatggtg
     5221 ggaagggcac gagcgtcgaa aagaggaacg ctttgtcaaa caagaagaaa aagctcgcca
     5281 aaaggctgag aaagaggcta gattagaaca agaagagact gaaaaagcct tactcgattt
     5341 gcctcctgtt gatatggaaa cgggtgaaat tctgacagag gaagctgttc aaaatcttcc
     5401 acctattcca gaagaaaagt gggtggaacc agaaatcatc ctgcctcaag ctgaacttaa
     5461 attccctgaa caggaagatg actcagatga cgaagatgtt caggtcgatt tttcagccaa
     5521 agaagccctt gaatacaaac ttccaagctt acaactcttt gcaccagata aaccaaaaga
     5581 tcagtctaaa gagaagaaaa ttgtcagaga aaatatcaaa atcttagaag caacctttgc
     5641 tagctttggt attaaggtaa cagttgaacg ggccgaaatt gggccatcag tgaccaagta
     5701 tgaagtcaag ccggctgttg gtgtaagggt caaccgcatt tccaatctat cagatgacct
     5761 cgctctagcc ttggctgcca aagatgtccg gattgaagca ccaatccctg ggaaatccct
     5821 aatcggaatt gaagtgccca actccgatat tgccactgta tctttccgag aactatggga
     5881 acaatcgcaa acgaaagcag aaaatttctt ggaaattcct ttagggaagg ctgttaatgg
     5941 aaccgcaaga gcttttgacc tttctaaaat gccccacttg ctagttgcag gttcaacggg
     6001 ttcagggaag tcagtagcag ttaacggcat tattgctagc attctcatga aggcgagacc
     6061 agatcaagtt aaatttatga tggtcgatcc caagatggtt gagttatctg tttacaatga
     6121 tattccccac ctcttgattc cagtcgtgac caatccacgc aaagccagca aggctctgca
     6181 aaaggttgtg gatgaaatgg aaaaccgtta tgaactcttt gccaaggtgg gagttcggaa
     6241 tattgcaggt tttaatgcca aggtagaaga gttcaattcc cagtctgagt acaagcaaat
     6301 tccgctacca ttcattgtcg tgattgtgga tgagttggct gacctcatga tggtggccag
     6361 caaggaagtg gaagatgcta tcatccgtct tgggcagaag gcgcgtgctg caggtatcca
     6421 catgattctt gcaactcagc gtccatctgt tgatgtcatc tctggtttga ttaaggccaa
     6481 tgttccatct cgtgtagcat ttgcggtttc atcaggaaca gactcccgta cgattttgga
     6541 tgaaaatgga gcagaaaaac ttcttggtcg aggagacatg ctctttaaac cgattgatga
     6601 aaatcatcca gttcgtctcc aaggctcctt tatctcggat gacgatgttg agcgcattgt
     6661 gaacttcatc aaggctcagg cagatgcaga ctacgatgag agttttgatc caggtgaggt
     6721 ttctgaaaat gaaggagaat tttcggatgg agatgctggt ggtgatccgc tttttgaaga
     6781 agctaagtct ttggttatcg aaacacagaa agccagtgcg tctatgattc agcgtcgttt
     6841 atcagttgga tttaaccgtg cgacccgtct catggaagaa ctggagatag caggtgtcat
     6901 cggtccagct gaaggtacca aacctcgaaa agtgttacaa caataaaaaa atagcttctt
     6961 tccgagtttg gagggaagct attttagtgg ctattgatta cttttatact cttcgcaaat
     7021 ctcttcaaac cacgtcagct tcgccttgcc gtactcaagt actgtctacg gctagcttcc
     7081 tagtttgctc tttgattttc attgagtatt attttctgaa gttggagtat tgggctgttt
     7141 ttcattttca gtggcaggtt tacttgtagc aggtgtagta gagtcctgag ttgctgtttt
     7201 ctgatcttct tttttctctt ccttgacgct agattttggc gtttcttctt gttgtgtttt
     7261 ttcttgagaa gtgttatttt ccttatttgg agtagtattt tcctgagggg attctttttg
     7321 aatttctttg acaaaggttg tcgtctggct tgtcgtaggt tcttttttag tatttttatt
     7381 attattatcc aaggcgttca tgatcgtgat acccgcggta atcaaaccaa ggatactacc
     7441 gatagtagta atggtttttt gaaaaacagt aaagcctttt ttgatatgtt ctgttttcgg
     7501 ttttgaagtt ctacgatagt tagacataca
//
LOCUS       DAVLWA010000087         7245 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLWA010000087 DAVLWA010000000
VERSION     DAVLWA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.087"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(366..1340)
                     /gene="pta"
                     /locus_tag="VYN80_001735"
     CDS             complement(366..1340)
                     /gene="pta"
                     /locus_tag="VYN80_001735"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1541608.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1384..2280)
                     /locus_tag="VYN80_001736"
     CDS             complement(1384..2280)
                     /locus_tag="VYN80_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1541609.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2277..3095)
                     /locus_tag="VYN80_001737"
     CDS             complement(2277..3095)
                     /locus_tag="VYN80_001737"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1541610.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3079..3750)
                     /locus_tag="VYN80_001738"
     CDS             complement(3079..3750)
                     /locus_tag="VYN80_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1541611.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3878..4447
                     /locus_tag="VYN80_001739"
     CDS             3878..4447
                     /locus_tag="VYN80_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1541612.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4560..5519
                     /locus_tag="VYN80_001740"
     CDS             4560..5519
                     /locus_tag="VYN80_001740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1541613.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5529..6644
                     /locus_tag="VYN80_001741"
     CDS             5529..6644
                     /locus_tag="VYN80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1541614.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            6649..6996
                     /locus_tag="VYN80_001742"
     CDS             6649..6996
                     /locus_tag="VYN80_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1541615.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            7004..7219
                     /locus_tag="VYN80_001743"
     CDS             7004..7219
                     /locus_tag="VYN80_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1541616.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
BASE COUNT         2235 a         1551 c         1353 g         2106 t
ORIGIN      
        1 cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt catcgccctg
       61 aacatcaaaa aagaaaggac aaaatttgtc ctttctcgag cttagctgac ttcaacccac
      121 tacagttgac aaagagccaa aaaattctct tttcatactc atttaaaatc aaaaagggaa
      181 tttatcgttt tacggatatg tatattacag atttctatag gtcgtgtata gacaggatat
      241 catcgcttgg aagttttcct ttttgacacc aatcagtaat ttagttctca ggaattaatg
      301 gcagtgcttt tcgtacatag tttcataact acagtatagc atagtatatc actttatagt
      361 tttcactatt gatgaactgc ttgagctgct gtgatgaggg tcaacttgta aacatcatct
      421 gcattacatc cacgagaaag atcattaact ggcttgttta aaccttgcaa aacaggtcct
      481 acagccgcaa agccacccag gcgttcagcc atcttgtaac cgatatttcc tgcctcgata
      541 cctgggaaga tgaagacatt tgcttgacca gctactgtac ttcccggagc tttcagagct
      601 gcagtttcgg gaacaaaggc cgcatcaaat tgcaactcac catcgatttc aaggtcagga
      661 cgcaagtcgt gagcaatttt agttgcttca acgaccttat caacgctttc accaaaccct
      721 gaacctttag tagaatagct caacatggca attttaggtt cgatgccaaa catcttagct
      781 gtgattgctg agttgatggc aatttcagcc aaggcttctg catctggatt gatattaatg
      841 gcacagtctc caaataggta acgttccgta ccacgaacca tgaggaaggc tcctgaagta
      901 cgagttacat ttggacgagt tttgatgatt tgtagagctg ggcgaactgt tgaagctgtt
      961 gagtgaatcg ctcctgacac cattccatca accaagccca agtaaaccaa catcacacca
     1021 aagtagttga catcttcaac caaaaccttg cgtacatctt cttcagtcat tttgcccttg
     1081 cgacgctcca ccaaggcaga aaccatttct tcaaattgag gataatgttg agggtcgatg
     1141 acctcataac catccatgat tccttcaatt tcaagataaa ttttaatttt ttcaggattt
     1201 ccaagcaaaa caggaatcac ttctgtttct tttactaagc gttttgttgc ttgaagaata
     1261 cgaggctctt ccccttcagg gagaacgata cgagcatttt taccaacaag gttggctttg
     1321 agactttcaa aaacttccat gagttttctc ctttaagata ataattatac tctgagacag
     1381 tttttataga gtattagttg ataactgggt aataaggtta ctgaaatcat ccggcaaggg
     1441 actttctaac tgcaagtctt gctctaaaaa tggatgataa aaggataggt aatggcaatg
     1501 cagagcctga cgttgaatac catcttccag actaccacca tacaaatcat ctcccagcaa
     1561 aggaaaaccg atatgagaaa aatggactcg gatttgatgg gttcgaccag tgtgcaggtg
     1621 aatatagacc aagtgaatat ttccataaga agctacaatc ttgtatgaag tatgggcata
     1681 ctttccgcct ttagccactc gtctggtaat aatggaatct tcatcacgcg caatcggagc
     1741 aataatttcc ccttctggtt ccaaatgtcc atctccctta accaaagcaa agtagcgttt
     1801 ctcgatagat ttcttctgca actgcttgtc taatcgtgca tgggcataac cgtgcttggc
     1861 aaagagcatc aagccagaag tgtccctatc tagtctggta acaatgtgaa cctgctgatt
     1921 ttcataattt tgcttgacat agtaaccctt gataaaattg gcaatggtat tagagtgatt
     1981 aacactagga atagaagcca ctccataggg tttattcaag actagaaagt ggtcatcctc
     2041 atagagaata tctaatggaa gctcaatagc ctccaaggtt tcaaagcctt tctcagcggg
     2101 aatgtcaatg gtaacgtagt ctccaacgtc caatagatac gttgcatttt gcggttgatt
     2161 attgaccaga atagctccac ctcgaaactt aatcttggcc agcaatccct tagaaacctc
     2221 gtgctttttt aagaaggtct taaccttgac atgttcatct gcgataaatt caaacctcat
     2281 tcatccacct caccgataaa ggcatcctta acacggttcc agaaactggt atggctagga
     2341 gtcgcgacaa agtgaatctt atgatgggcg atttgatact caatacgctc aatattacgg
     2401 aaagaataaa cgctattgtc aaccgaaata gtatgataat cgtttcttgt tggaataagt
     2461 tcaatcttat ccttcttagg cacaataatg gaagagccca gtgttcgata gacacgatta
     2521 ttaaggctgg caatttccgt taattgcaaa gcttcaatgg tagggtgtaa aacagcaccg
     2581 ccaagagact tgttataggc agtactacca gtcggtgtcg aaactgttag cccgtctcca
     2641 cgaaaacgtt caaagggaac accatttatt acaatatctg ccaccatggt tcgatcagac
     2701 ctgcggatgc tggcttcgtt gagtgctctg aaaatcttaa cttcaccatt ttcaagaaag
     2761 accttcacat tcagaacagg gtaagaaacc cttgccccag tatctagctg caaattagtc
     2821 actagcttgt ccaactcaaa atcacgataa tctgtataga agcccaaatg tccagtgtga
     2881 agaccgataa agcggacctt gtcaagctga ttttcgtact tatgaaaggc cgacaagagc
     2941 ataccatccc cgccaatgga aatgacaata tccggattgg tatcattgag tataaactga
     3001 tttctcttca aacgatctcg caattcatac aaaacccttt gactctgcgg ttttctattg
     3061 gctatcagat caattcgttt acctgtattc ttcatctgta tcgtcactgt ttcctacacc
     3121 gtcatttaat tttctactca aaggatcaaa aagtgcctga gcttcttgga tatcatcacg
     3181 aatttcaccc atttcttcat ccaactgatg ggcgattcta gctgtaattt ccagtcgctt
     3241 cttaatctca tctgggaaat ccccttggta cttgtagttg agagaatgtt ctatcgttgc
     3301 ccagaaattc atggccaaag tacgaatttg aatttctgcc aaaatagtct taactccatt
     3361 gatggtatca accgtatatt ctactaccac atgataggaa cgatagcctg atgcttttct
     3421 atgagtaatg taatctcgct cctgtatgat tcgcatatcc tgacgcttgt gcaaaatatc
     3481 cactacttcc ttgacgtcat ctacaaactg aaccatcaca cgtaagccag caatatcctg
     3541 caaatcgtgt tccaaggtcg cataagtaat gccacgacga gccatttttt ctttgatgct
     3601 ctcaattggc ttaactcgac cggtcacaaa ctcaattgga gaatgcttat tttgcttacg
     3661 atattgctta cgaataccac gaagtttaat ctttaactca ccaacagctt gaatgtaagg
     3721 atctagaaat tcttcccatt ctaaggtcat atattctccc ttgttctcat attatccgtc
     3781 aaaaatatct ttgatttttt ctccagattc atatacaata aatacaatgc ttattatacc
     3841 ataaatccgc tttcaacgat aaaaaaaggt aagaaaaatg aaacatttag aaattgaatt
     3901 gaaaacacta ttgaaaaaag atgaatacaa tcgtctaaaa gaccagttca caggtgtcac
     3961 tcctgttctt caaacaaatt actacatcga cacgcctgat tttgaactgc gagaaaagaa
     4021 agtcgctatg cgcattcgaa cctttgaaga ctgggctgag ttgacactca aagtcccgca
     4081 gagtgttgga aacatggaat acaaccaaaa attgcaacta aaagatgctg aagattatct
     4141 gagcaaggaa gaacttcctc aaggactagt actggatgaa ttggcgaaac atggtatcca
     4201 aagtaagaac tggcaggttc ttggttgtct aacaacgctt cgctatgaaa tgaaaacagc
     4261 tattggtctc atggcactgg atcagagccg atactttgat atgacagatt acgaattgga
     4321 gcttgaagtg gaaaatcatg agcaaggcaa acaggatttc cgacaatttt tagagaaaaa
     4381 tcagatttcc taccaaaaag ctccttcaaa attggttcga tttgtcaaaa gtatgaaaaa
     4441 tagctgaaat aatctccatt ttttggtaaa atagaaaaga taaaaataca caaatcccag
     4501 ttcatatatg taaggtaaat ataactagga tttataaagt ttacagagga cggtctataa
     4561 tgtcagatag aaaaaacatg aaacttttcg cactcaactc taaccaagag attgcacaaa
     4621 aaattgccca agctgttggt gtcccacttg gaaaactatc atcacgtcaa ttttcagacg
     4681 gagaaatcca agtaaatatc gaagaaagtg tccgtggtta tgatgtttac atcatccaat
     4741 caacaagttt ccctgtcaac aaccacctaa tggaattgtt aatcatggtc gatgcttgtg
     4801 tgcgtgcaag tgcccacagt atcaacgttg tccttccata ttttggctat gcacgtcaag
     4861 accgcattgc ttgtcctcgt gagccactta cagcaaaact agttgccaat atgctggtta
     4921 aggctggagt tgatcgtatc ctgactcttg atttgcatgc cgttcaggtt caaggtttct
     4981 ttgatattcc agtggataat cttttcactg ttcccctatt cgcaaaacat tactgcgata
     5041 aaggattgct tggttcagat gttgttgtcg ttagccctaa aaattcaggt gtcaaacgtg
     5101 cgcgtagcct ggctgaatat cttgatgctc ctatcgccat tatcgactac cctcaagacg
     5161 atgcaactcg taacgaaggt tatattattg gtgatgttga aggtaagaaa gctatcttga
     5221 ttgatgatat tttaaataca ggacgtacct tctctgaagc ttctaaaatc gttgaacgtg
     5281 aaggagctac agaaatttat gctgtttcta gccacggtct cttcgtcgag ggagctgctg
     5341 agcttcttga caatactaat attaaagaaa ttcttgtgac tgattcagta gcaacaaaag
     5401 aaaaaactcc taaaaatgta tgctacatca ctgctagtga gttaattggt gatgctatcg
     5461 tccgtattca cgaaagaaaa ccagtcagcc cactctttgc ctacaataaa aagaaataag
     5521 gtgattcttt gatttatttg gacaatgctg caacgactcc tatgtcagca gttgctattt
     5581 cagctatgac caaggttatg caagaaaccc acggaaatcc ttctagtatt catggtcatg
     5641 gtcgtcaagc tggcaaactc ttgcgagaag cccgtcagga actagcccag ttactaagga
     5701 caaaacctca acatatcttt ttcacttctg gtgggactga aggcaataat actaccatca
     5761 ttggctactg tcttcgtcac caagaacaag gaaaacatat catcacaact gccatcgagc
     5821 accatgctgt ccttgaaaca attgattact tggttcaaca ctttgggttt gaagcaacca
     5881 ttatccagcc agaaaatcaa gaaatcacag cccagcaaat tcaaaaggct ttacgtgacg
     5941 atacgatttt ggtttctact atgtttgcca ataatgagac aggaaaccta cttcccatcg
     6001 ctgaaattgg ccaaatactc aagcaacacc ctgctgccta tcatgttgat gcagttcagg
     6061 ctattggtaa aatcccaatt cattcagaag aattgggcat tgattttctc actgcttctg
     6121 cccacaaatt ccatggtcct aagggaatcg gttttctcta cgcatctagc atggactttg
     6181 attcctatct acatggcgga gatcaggaac agaaaaaacg tgcaggaact gaaaatctgc
     6241 ctgccatcgt aggcatggtt gcagccctaa aagaagacct agaaaaacaa gaagaacatt
     6301 ttcaacatgt acaaaatcta gaaactgcct ttctggcaga gctggagggc attcagtatt
     6361 acttgaatag aggaaaacat catctccctt atgttctcaa tattggattt cctggtcaga
     6421 aaaatgacct cttactcctt cggctagatt tagctggaat ttcaatctct actggctcag
     6481 cctgtactgc aggcgttgtc caatccagcc atgttcttaa agccatgtat ggcgcaaatt
     6541 cagaacgctt gaaggaatcc cttcgcatca gtttgtcgcc acaaaatacc gttgaagacc
     6601 tacaaaccct cgcaaaaacc ttaaaagaaa ttatcggagg ttagccatat ggcatttgaa
     6661 aaaatcattc agttaaaaaa ttgtcgttac gattacactc ttagcccttc tgttaaaaaa
     6721 ttcaccctca aagataacac cttttttgaa actaaggttg gtaactatga actgactcgc
     6781 cttttggaaa aagtgccaaa cagcggtgaa ggcttccaac tcaaaatcat cattaacaag
     6841 gaacttacag gggctaaaat caatatcact gacaagtttg gccttcgtct agttgatatt
     6901 ttcaaatcag aagaccacca tattcatcag gaaaaattct acttcctcat ggatagcttg
     6961 gtagaacgtg gtgtctttac aaaatcggaa agatagagcc aaaatgtttg aactgaccta
     7021 taaggactgc tatcatgtag agcgtactct caagtatgag gatcatgagg ctctcatgtt
     7081 gactttgtca ggatgtgtaa ccttaccaga tacactttat gtgacttctc tgacttttag
     7141 gggcaaaaaa gtttatcagg ggctggtcgg agacctctac cgttttctat ctcatgcaga
     7201 ttttttacat caaaactaag atttttctta gttttttctt atttt
//
LOCUS       DAVLWA010000088         7200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLWA010000088 DAVLWA010000000
VERSION     DAVLWA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.088"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(85..1047)
                     /gene="nrdF"
                     /locus_tag="VYN80_001744"
     CDS             complement(85..1047)
                     /gene="nrdF"
                     /locus_tag="VYN80_001744"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1541617.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1235..3394)
                     /gene="nrdE"
                     /locus_tag="VYN80_001745"
     CDS             complement(1235..3394)
                     /gene="nrdE"
                     /locus_tag="VYN80_001745"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1541618.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3475..3693)
                     /gene="nrdH"
                     /locus_tag="VYN80_001746"
     CDS             complement(3475..3693)
                     /gene="nrdH"
                     /locus_tag="VYN80_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1541619.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPEKLKQLA"
     gene            4044..4307
                     /locus_tag="VYN80_001747"
     CDS             4044..4307
                     /locus_tag="VYN80_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1541620.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4313..6046
                     /gene="ptsP"
                     /locus_tag="VYN80_001748"
     CDS             4313..6046
                     /gene="ptsP"
                     /locus_tag="VYN80_001748"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1541621.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            6301..>7200
                     /gene="phtA"
                     /locus_tag="VYN80_001749"
     CDS             6301..>7200
                     /gene="phtA"
                     /locus_tag="VYN80_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1541622.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLS"
BASE COUNT         2226 a         1343 c         1544 g         2087 t
ORIGIN      
        1 agcaaactag gaagctagcc gcaggctgta cttgagtacg gcaaggcgaa gctgacgtgg
       61 tttgaatttg attttcgaag agtattagtc caaaccgtag ttgtagtcat catcttgcat
      121 ggcttcaact tcgccaagaa ggtaaccatt tccgacttga gagaagaagt catggttgga
      181 agttcctgtt gaaataccgt tcataacgat tgggttgaca tcttcagctg aatctgggaa
      241 aagtggatct tgtcccatgt tcatgagagc tttattggca ttgtagcgaa ggaaggtttt
      301 aacctcttca gtccaaccaa caccgtcata aagactctct gtgtagcctt cttcattttc
      361 ataaagagta tagagtaggt cgtacatcca ttctttgagt ttttcttgct cttcttcagg
      421 caattcattg aaaccaagtt ggaatttgta accaatgtag gttccgtgaa cagactcgtc
      481 acgaataatc aatttaatga tttctgcaac gttggctagt ttgttgttac cgagatagta
      541 gaggggagtg aagaaaccag agtagaagag gaaggtttcg aggaagacgc tggcaacttt
      601 cttttcaagt gggctgccgt ttaggtagat ttcgttgaca atctcagcct tcttttgtag
      661 gtaaggattg gtattagtcc attcgaaaat ttcttcaatc tcagccttag tattcaaggt
      721 agaaaagatt gatgagtaag atttagcgtg gacagattcc ataaattgga tgttattgaa
      781 aacagcttcc tcatgtggtg tacggatgtc tgcgcgaagg gcttgaaccc cagtttcaga
      841 ttgcatagtg tcaagaaggg ttaaaccacc aaaaactttt ccgaccaagt ctttctcttt
      901 gttagatagc tttctccagt catccaagtc gtttgataag ggaatacgtg tatcgagcca
      961 aaattgctcc gtcagttttt cccaagttga tttgtcgatg acatcttcga tggcattcca
     1021 gttaatggct ttgtagtaag tttccattta aaatctcttt ctgtgtttag tattgcgaac
     1081 tcacaattat ttctacttta ccataattct ataggagtat cgcacaaaaa gtcggaagcc
     1141 cgacttttaa aatgttacat aaattatgtt atgacatagt agatttgatt ttatcagtgc
     1201 tgcttaggga aaaatagtgt ttctatgcta gaaactaaat cacacagctt tcacattggt
     1261 tggcgccgac ttctccaccg tcatctgtaa aggtacggac gtagtagata gacttgattc
     1321 ccttgttaaa ggcatagtta cgaaggatgg acaagtcacg tgtcgtttgt ttattttccc
     1381 tcttccattc gtaaaggcct tttggaatgt cactacgcat gaagagggtg agtgaaagtc
     1441 cttgatccac gtgttcagtc gcagcagcgt aaacatcgat gactttacgc atatccatat
     1501 cgtaggcaga agtgtagtaa ggaatggttt ctgtagacaa gccagcagca gggtagtaga
     1561 ttttaccaat tttcttttcc tgacgttctt caatacgttg cgtaatcggg tggatagaag
     1621 cagaaacatc gttgatgtag ctgatagaac catttggcgc tacagcaagg cgattttggt
     1681 ggtaaagacc atcctcttga accttgtcgc gaagttcagt ccaatcagca acaccaggga
     1741 taaaaacatt tttgaagagt tctttaacac ggtctgatgt tggaacaaat tcaccagtta
     1801 catacttgtc aaagtaactt ccgttagcat agtctgattt ttcaaagttg tggaaggtaa
     1861 taccacgttc acgcgcaata ttgtttgact ctaccaaggt ccagtagttc ataagcataa
     1921 agtagatgct tgtaaattca acagactcag gtgaaccata ttcaatgagt tgttgggcaa
     1981 ggtagctgtg cagtcccatg gcaccgagac caaaggtgtg ggcttggcta tttccatggt
     2041 caatcgttgg tacagctacg atatgtgaac tatctgtaac gaaagtaagg gcacgaacca
     2101 tagcacggat agaacgacca aaatcaggtg aagtcatcat gttaaccacg ttggttgaac
     2161 ccaggttaca tgaaacatct gttcccattt gaaggaattc ttgagcatcg ttgatcaagc
     2221 ttggttcttg aacttgaaga atctcagaac acaagttact catgataatc tttccatcga
     2281 caggatttgc acggttagcc gtatctatgt tgactacata aggatagcca gactcttgtt
     2341 gcaatttaga gatttcagtt tccaaatccc gcgccttgat ttttgtcttg cgaatatttg
     2401 gatttgcgac caattcatcg tatttttcag taatgtcgat gtaattgaat ggcacaccgt
     2461 attctttttc tacagagtaa gggctgaaga ggtacatttc ttcattttta cgagccaatt
     2521 cgtagaattt atcaggtact acaacaccaa gtgatagagt cttgacacgt actttttcat
     2581 cagcgttttc tttcttagtt gaaaggaaag cgatgatatc tgggtgaaag acgttgaggt
     2641 agacaacacc ggcaccttga cgttgcccca attggttgga gtaagagaag ctgtcttcaa
     2701 aaagcttcat aacaggaacg acacctgaag cagctccttc atagcctttg ataggtgcac
     2761 cagcttcacg aaggttgctg agggtaattc ccacaccacc accaatacgt gaaagttgaa
     2821 gagctgagtt gatagaacgt ccgatagagt tcatatcatc cgtcacttgg attaggaaac
     2881 aagataccaa ctccccacga cgagcacgtc cagcattcaa gaaggaagga gtagcaggtt
     2941 ggtagcgttg gtggatgatt tcattggcaa tatcgattgc aacagcttca ttcccatcag
     3001 cgaaataaag ggcgttgaag aagacacggt cttccatatt ttcaagatag tattcaccgt
     3061 cattagtctt taaggcatat tgattgtaaa atttataagc tgccatgaat gacttgaatt
     3121 ggaagttttg gtctttgata aattgagcca attcttccaa gaactctgga cggtatttct
     3181 tgataaaggc tgtttcgatg tagttgtgtt caatgaggta attgattttg tctttgattg
     3241 aatcaaaaac catagtgttt ggaactacat tttctttaaa gaaagcatcc aaggcttcct
     3301 tatctttatg aagcatgatt tgtccattaa caggacggtt aatttcgtta ttaagacgga
     3361 agtaagtcac gtcttcaaga tgttttaatc ccataaaatt tcccttatct aattacaaaa
     3421 gaaaggcttc taagttagcc ctaaaagcag tttcttctgg atgatgtact aagattatgc
     3481 taattgtttc agtttttctg gttggaaacc tgaaaagact tcagttggtg tttggataac
     3541 aggagctgcg ctaaaaccga gctctttaac ttgatcgacg tactcaggtt gctcatcaag
     3601 attgatttca cgataagaga cattattact gtccaagaaa cgcttggtca ttttacattg
     3661 gacacaattg tttttagaat aaacggttac cattgtgtaa ctcctcttca aaatttaata
     3721 ctatcttagt atatcagaaa ataaaatttt gtcaatgatt taaactaaat atagtggtgt
     3781 tcttttgttg tttttatata taaacacaat atatagtgtt tgtattgtga aatagcaatg
     3841 attatcctat aaattgtttt tgaagaaact atatcctgtg ttttgttatc taggatagaa
     3901 gattttcagc aagttatctg aaaggaaatt gaaggaaaat ccagaaaatg cttgaaaaaa
     3961 atcctagaag atggtataat actaaattgt aagggttatc acatataact caaaaaaaga
     4021 aagaacaaaa ggagagtcaa actatggctt ctaaagattt ccacgtagtg gcagaaacag
     4081 gtattcacgc acgtccagca acattgttgg tacaaactgc tagcaaattt gcttcagata
     4141 tcactcttga gtacaaaggt aaatcagtta accttaaatc aattatgggt gttatgagtc
     4201 ttggtgttgg tcaaggtgct gacgtaacta tctcagctga aggtgcagat gcagatgacg
     4261 ctatcgctgc aatctcagaa acaatggaaa aagaaggatt ggcataaggg aaatgacaga
     4321 aatgcttaaa ggaatcgcag catctgacgg tgttgcagtt gcaaaagcat atctactcgt
     4381 tcagccggat ttgtcatttg agactattac agtcgaagat acaaacgcag aagaagctcg
     4441 ccttgatgcc gctctacagg catcacaaga cgagctttct gttattcgcg agaaagcagt
     4501 aggtacgctc ggtgaagaag cagctcaagt ttttgatgct cacttaatgg ttcttgctga
     4561 cccagaaatg atcagccaaa tcaaggaaac tatccgtgcg aagaaagtga atgcagaagc
     4621 aggtctgaaa gaagttacag atatgtttat cactatcttt gaaggcatgg aagacaaccc
     4681 atacatgcaa gaacgcgcag cggatatccg cgacgtgaca aaacgtgtat tggcaaacct
     4741 tcttggtaaa aaattgccaa acccagcttc tatcaatgaa gaagtgattg tgattgcgca
     4801 tgacttgact ccttcagata cagctcaatt ggacaaaaac tttgtaaaag cttttgtaac
     4861 caacattggt ggacgtacaa gccactcagc tatcatggca cgtacacttg aaattgctgc
     4921 tgtattaggt acaaacaaca tcactgaaat cgttaaagac ggtgacatcc ttgctgttaa
     4981 cgggatcact ggagaagtga ttatcaaccc gacagatgaa caagcggcag aatttaaagc
     5041 agctggtgaa gcctatgcga aacaaaaagc tgaatgggca cttttgaaag atgctcaaac
     5101 agtgactgct gacggtaaac acttcgagtt ggctgccaat attggtactc caaaagacgt
     5161 tgaaggtgtt aacaacaacg gtgcagaagc tgttggactt taccgtacag agttcttgta
     5221 catggattct caagacttcc caactgaaga tgagcagtat gaagcataca aggctgttct
     5281 tgaaggaatg aacggtaaac ctgttgtcgt tcgtacaatg gatatcggtg gagataagga
     5341 acttccttac ttcgatatgc ctcacgaaat gaacccattc cttggattcc gtgctcttcg
     5401 tatctctatc tctgagactg gagatgctat gttccgcaca caaatccgtg ctcttcttcg
     5461 tgcgtctgtt cacggtcaat tgcgtatcat gttcccaatg gttgcgctct tgaaagaatt
     5521 ccgtgcagcg aaagcagtct ttgatgaaga aaaagcaaac cttcttgctg aaggtgttgc
     5581 agttgcggat aacatccaag ttggtatcat gatcgagatt cctgcagcgg ctatgcttgc
     5641 agaccaattt gctaaagaag ttgacttctt ctcaattggt acaaacgact tgatccaata
     5701 tacaatggca gcagaccgta tgaacgaaca agtttcatac ctttaccaac catacaaccc
     5761 atcaatccta cgcttgatta acaatgtgat caaagcagct cacgctgaag gtaaatgggc
     5821 tggtatgtgt ggtgagatgg ctggtgacca acaagctgtt ccacttcttg tcggaatggg
     5881 cttggatgag ttctctatgt cagcaacatc tgtacttcgt acacgcagct tgatgaagaa
     5941 actcgacaca gctaagatgg aagagtacgc aaaccgtgcc cttacagaat gctcaacaat
     6001 ggaagaagtt cttgaacttc aaaaagaata cgttaatttt gattaatcga aaagtccctg
     6061 caactcagtt acagggactt tttttgatat tttaaaaaga attttcaaga aaatctttct
     6121 tataaaaagt ccaaccttga aaaagtagtg gtcagaacaa aaaatactta aatggttcat
     6181 aaaattcttg acaagttgga gatttaggag taaactatta accagttaag taatagagag
     6241 gagtttctgc aatttagaaa tgaattgcaa ctagaaatat caaatagaaa gagagtttcg
     6301 atgaaaatta ataagaaata ccttgttggt tctgcggcag ctttgatttt aagtgtttgt
     6361 tcttacgagt tgggactgta tcaagctaga acggttaagg aaaataatcg tgtttcctat
     6421 atagatggaa aacaagcgac gcaaaaaacg gagaatttga ctcctgatga ggttagcaag
     6481 cgtgaaggaa tcaatgctga gcaaatcgtc atcaagataa cagaccaagg ctatgtcact
     6541 tcacatggcg accactatca ttattacaat ggtaaggttc cttatgatgc gattttcagt
     6601 gaagaattac tcatgaaaga tccaaactat aagctaaaag atgaggatat tgttaatgag
     6661 gtcaagggtg gatatgttat caaggtagat ggaaaatact atgtttacct taaggatgct
     6721 gcccacgcgg ataacgtccg tacaaaagag gaaatcaatc gacaaaaaca agaacatagt
     6781 caacatcgtg aaggtgggac tccaagaaac gatggtgctg ttgccttggc acgttcgcaa
     6841 ggacgctata ctacagatga tggttatatc tttaatgctt ctgatatcat agaggatact
     6901 ggtgatgctt atatcgttcc tcatggagat cattaccatt acattcctaa gaatgagtta
     6961 tcagctagcg agttagctgc tgcagaagcc ttcctatctg gtcggggaaa tctgtcaaat
     7021 tcaagaacct atcgccgaca aaatagcgat aacacttcaa gaacaaactg gctaccttct
     7081 gtaagcaatc caggaactac aaatactaac acaagcaaca acagcaacac taacagtcaa
     7141 gcaagtcaaa gtaatgacat tgatagtctc ttgaaacagc tctacaaact gcctttgagt
//
LOCUS       DAVLWA010000089         6822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLWA010000089 DAVLWA010000000
VERSION     DAVLWA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.089"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            172..830
                     /locus_tag="VYN80_001750"
                     /pseudo
     CDS             172..830
                     /locus_tag="VYN80_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            985..1185
                     /locus_tag="VYN80_001751"
                     /pseudo
     CDS             985..1185
                     /locus_tag="VYN80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1210..1626
                     /locus_tag="VYN80_001752"
     CDS             1210..1626
                     /locus_tag="VYN80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541623.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1857..2564
                     /gene="ccdA2"
                     /locus_tag="VYN80_001753"
     CDS             1857..2564
                     /gene="ccdA2"
                     /locus_tag="VYN80_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1541624.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2575..3132
                     /gene="sdbB"
                     /locus_tag="VYN80_001754"
     CDS             2575..3132
                     /gene="sdbB"
                     /locus_tag="VYN80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1541625.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDAPDFTLQSMDDKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3506..4897
                     /locus_tag="VYN80_001755"
     CDS             3506..4897
                     /locus_tag="VYN80_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1541626.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            5040..5957
                     /gene="adcAII"
                     /locus_tag="VYN80_001756"
     CDS             5040..5957
                     /gene="adcAII"
                     /locus_tag="VYN80_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1541627.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDREHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5965..>6822
                     /gene="phtB"
                     /locus_tag="VYN80_001757"
     CDS             5965..>6822
                     /gene="phtB"
                     /locus_tag="VYN80_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1541628.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT         2119 a         1216 c         1421 g         2066 t
ORIGIN      
        1 tattcttgtg aatgcttgct tttttgtggt tgcaatccgt ttacgagaat tgtttgtatt
       61 gttcttgttt caatttactt tatagtgctt tgaagttgga atgctacact ataacttcta
      121 aaatatagta aaatgaacca aaaatagtac acaatgtggt ataatcttct tatggcatat
      181 tcaatagatt ttcgtaaaaa agttctctct tattgtgagc gaacaggtag tataacagaa
      241 gcatcacacg ttttccaaat ctcacgtaat accatttatg gctggttaaa gctaaaagag
      301 aaaacaggag agctaaacca ccaagtaaaa ggaacaaaac caagaaaagt tgatagagat
      361 agacttaaaa actatcttac tgacaatcca gatgcttatt tgactgaaat agcttctgaa
      421 tttggctgtc atccaactac catccactat gcgctcaaag ctatgggcta cactcgaaaa
      481 aaagaaccac acctactatg aacaagaccc agaaaaagta gccttatttc ttaagaattt
      541 taatagatta aagcacctag cacctgttta gattgatgaa acaggattcg atacttattt
      601 ttatcgagaa tatggtcgct cattaaaaag tcagttaata agaggcaaag tatctggaag
      661 aagatatcag aggatttctt tggttgcagg tctaacaaat gaatggtgag ttaatcgctc
      721 caatggctta cgaagagacg atgacgagcg acttttttga agcttggttt cagaagtttc
      781 tcttaccaac attaaccaca ccatcggtta ttatagtaaa acgaaataag aataggacaa
      841 atcgatcagg acagtaaaat cgaattctaa caatgtttta gaagtccata tgtactattc
      901 tagtttcaat ataccataac tgctcctagt caacctgcaa gtcctgcccc agctccaagc
      961 tcagctccga ctcaagatcc tagagtggta gtaagcgctt cggctagctc aacgagcaca
     1021 caagctcaag agtaagttga caagtctgaa cttcgtgcct tgagtcaaga gttagaccaa
     1081 cgcttgaaag ctttggcaac agtatctgat ccgaaaatcg atgcaaccaa ggctgtcctc
     1141 ctagatgctc aaaaagctcc ggaagatagt gccttgacag agtaaggtct gcatactgca
     1201 gtagagtctg tgaaggctgc cttggactct ctcaaagatg tgaaagcaaa tgctagcgac
     1261 agcaagcctg cacaggacaa gaaggatgca aaacaaggaa cggaagatag taaggattca
     1321 gataagatga ctgaaacaaa ctcagttccg gcaggagtga ttgtggtcag tctacttgcc
     1381 ctcctaggcg tgattgcctt ctggctgatt cgccgtaaga aagagtcaga aatccagcaa
     1441 ttaagcacgg aattgatcaa ggttctagga cagctagatg cagaaaaagc ggataaaaaa
     1501 gtccttgcca aagcccaaaa ccttctccaa gaaacccttg atttcgtgaa agaagaaaat
     1561 ggctcagcag agacagaaac taaactagta gaggagctta aagcaatcct tgacaaactc
     1621 aagtaaaaca tgatttatga aatgtcagac ctaaaagaca ttttcgttca caaaagcagt
     1681 gacgagtaat ccttgtcgtt gctttttggc tattttgggg gataagatcc gccttgacta
     1741 gcaaaagaaa agattggtcc aatagtgccc gatacgattt tgcgtagcag aaagcttaca
     1801 aataaaattg aatatagtaa aataggatta gaattattaa gaaagttggt tctttattgg
     1861 aaacgatagt atttttaatc tctgtttttc tagcaggtgt tttatccttt ttttctcctt
     1921 gtatttttcc tcttctgcca gtctatgctg ggattttatt ggatgatcaa gaaagtgcaa
     1981 aaagcttttc tttgtttggg agaaaggttc tctggtcagg cttgattcga acactttgct
     2041 ttatcgctgg tatctctctc attttcttta ttctaggctt tggtgctggt tactttggtc
     2101 atattctcta tgcaaattgg tttcgatatg tcatgggagc tattattatc attttgggtc
     2161 ttcaccagat ggaaattttt catttgaaga aattagaagt tcaaaaaagt tttaccttta
     2221 aaaaatcaga ttctaatcgt tattggtcag cttttttact tggtattacc tttagctttg
     2281 gttggacacc ttgtattggt ccagttttaa gttctgtttt agcacttgcg gcttctggag
     2341 gcaatggtgc ttggcaagga gcgatttata ctctcattta cactctgggc atggcccttc
     2401 ctttcttggt cttggcacta gcttcaggtc tagtcatgcc atattttagt aaaatcaagc
     2461 gtcatatgat gctactaaag aaaattggtg gtttcctcat cgttttaatg ggaattttgt
     2521 tactattagg acaagtaaat gttctagctg gaatttttga ataaaggagg acaaatgaaa
     2581 aaagtaatgt ttgctggctt aagtctcttg tcattagttg tattgatggc ctgtggtgag
     2641 gaagaaacta aaaagactca agcagcacaa cagccaaaac aacaaacgac tgtacaacaa
     2701 atttctgttg gaaaagatgc tccagacttt acattgcaat ccatggatga caaagaagtt
     2761 aagttatctg attttaaggg taaaaaggtt tacttgaagt tttgggcttc atggtgtggt
     2821 ccatgcaaga aaagtatgcc agagttgatg gaactagcgg cgaaaccaga tcgtgatttc
     2881 gaaattctta ctgtcattgc accaggaatt caaggtgaaa aaactgttga gcaattccca
     2941 caatggttcc aggaacaagg atataaggat atcccagttc tttatgatac caaagcaacc
     3001 accttccaag cttatcaaat tcgaagcatt cctacagaat atttaattga tagccaagga
     3061 aagattggaa agattcaatt tggtgctatc agtaatgcgg atgcagaagc agcatttaaa
     3121 gaaatgaact agaatcgata aaatctgatt acaagatgta gtcagatttt ggtaacttct
     3181 aaaactaact tccagtttcc cacaacctag cttttagtat gagaaaaacg cgtgacatca
     3241 gtggaaatcc gtcttagact aacttccact ggttttcttt ttcttgatat ataagggttc
     3301 aaaagcgaaa agactcagaa aaagtgcacg acaaatagcc ctaaaacaga gtcgtcttag
     3361 agtaacttcc agttttggga gagagatgga agttactttg agaagttacg gtagtcagat
     3421 ttttttagaa aaacatttta taaatattca catatctcct atatttatgg taaaatagaa
     3481 ttatcagttt attttggagt caaagatgaa tatatttaga acaaagaatg ttagtttaga
     3541 taaaacagag atgcataggc atttgaagtt atgggatttg attttgctgg gtatcggagc
     3601 catggtaggg acaggcgtct ttacaatcac aggtactgca gctgcaacac ttgctggccc
     3661 agccctagtg atttcaatcg ttatttctgc cttgtgtgtg ggattatcag ccctcttttt
     3721 tgcagaattt gcctcgcgag tacccgctac aggaggtgcc tatagttacc tctatgctat
     3781 cttaggagaa ttccctgcct ggttggctgg ttggttaacc atgatggagt tcatgacagc
     3841 catatcaggc gtagcttcgg gttgggcagc ttattttaaa ggcttgctct ctcaatacgg
     3901 gatagccttt cctcaggctt taaatggtac ctttaatcct caagcaggga catttgttga
     3961 tttattgcct attcttgtct tggtcttggt gacttcgctt gttttactaa atgccaaggc
     4021 agccttacgc tttaattcga ttctagttat tttgaaattt tccgctttag ctctctttgt
     4081 tttagtagga atttggaata ttaagtttga taattggagc aattttgctc cttacggttt
     4141 tggacaaatc tatggagcta gtactggtat tatggctggt gcatccttga tgttcttcgg
     4201 ttttttggga tttgaatcca tctctatggc tgtagatgaa gtcaagactc ctcaaaaaaa
     4261 tattcctaga gggattgtct tatcgctttc tatcgtaacc attctctatg ccttggtgac
     4321 ccttgttttg actggtgtgg ttcactatag tcatttaaat gtcgatgatg ccgttgcctt
     4381 tgcccttcgt agtgttggga ttagttgggc agccaactat gtgtcattag tggctatctt
     4441 gaccttgatt acagtttgta tttcgatgac ctatgcccta tcgcgtatga tttacagttt
     4501 agcacgtgac ggcttagtgc ctgctgcctt taaggaactg acgaagacta gcaagatacc
     4561 aaagaatgct actatcttga caggtctagc ttcagcagta gcagcaggaa tgttcccact
     4621 agccagtatt gcagcctttt taaatatttg taccttagcc tacttgatca tgttggctta
     4681 tggtctgatt cgcttacgga aagaaaaagg aatgcccaaa gctggggaat ttaaaacacc
     4741 attggtacct ctgttaccaa ttttatcgat catcatttgt ttatccttca tgttacagta
     4801 taatatgaat acctggattg ctttcctagt tgcgttgtta gtaggaagta ttatttactt
     4861 cacttatggc tataagcatt ctaccataga agaataaagt gaaagtctat tgagttgttg
     4921 acactcagta gatttttata tgtgaaagaa atttcaaatt ttttctaaaa atatcttgac
     4981 agattaaatt ttcaggagta gaatatttac tagttaatta aatggttaag gagttgttca
     5041 tgaagaaaca aaatttattt ttagtcctgt taagtgtctt tcttttgtgc ttgggggctt
     5101 gtggtcaaaa ggaaagtcag acaggaaagg ggatgaaaat tgtgaccagt ttttatccta
     5161 tctacgctat ggttaaggaa gtatctggtg acttgaatga tgttcggatg attcagtcaa
     5221 gtagtggtat tcactccttt gaaccttcgg caaatgatat cgcagccatc tatgatgcag
     5281 atgtctttgt ttaccattct catacactcg aatcttgggc aggaagtctg gatccaaatc
     5341 taaaaaaatc caaagtgaag gtcttagagg cttctgaggg aatgaccttg gaacgtgtcc
     5401 ctggactaga ggatgtggaa gcaggggatg gagttgatga aaaaacgctc tatgaccctc
     5461 acacatggct agatcctgaa aaagctggag aagaagccca aattatcgct gataaacttt
     5521 cagaggtgga tagggagcat aaagagactt atcaaaaaaa tgcgcaagcc tttatcaaaa
     5581 aagctcagga attgactaag aaattccaac caaaatttga aaaagcgact cagaaaacat
     5641 ttgtaacaca acatacagcc ttttcttatc tagcgaagag atttgggctt aatcaacttg
     5701 gtattgcagg tatctctcct gaacaagaac caagtccacg acaactaaca gaaattcagg
     5761 aatttgttaa gacctataag gttaaaacga tttttacaga aagtaacgct tcttcaaaag
     5821 tagctgaaac tcttgtcaaa tcaacaggtg tgggtcttaa aactctgaat cctttagagt
     5881 cagacccaca aaatgacaag acctatttag aaaatcttga agaaaatatg agtattctag
     5941 cagaagaatt aaagtgagga aagaatgaaa attaataaaa aatatctagc aggttcagtg
     6001 gcagtccttg ccctaagtgt ttgttcctat gagcttggac gttaccaagc tggtcaggat
     6061 aagaaagagt ctaatcgagt tgcttatata gatggtgatc aggctggtca aaaggcagaa
     6121 aacttgacac cagatgaagt cagtaagagg gaggggatca acgccgaaca aattgttatc
     6181 aagattacgg atcaaggtta tgtgacctct catggagacc attatcatta ctataatggc
     6241 aaggttcctt atgatgccat catcagtgaa gagctcctca tgaaagatcc gaattatcag
     6301 ttgaaggatt cagacattgt caatgaaatc aagggtggtt atgtcattaa ggtaaacggt
     6361 aaatactatg tttaccttaa ggatgcagct catgcggata atattcggac aaaagaagag
     6421 attaaacgtc agaagcagga acgcagtcat aatcataact caagagcaga taatgctgtt
     6481 gctgcagcca gagcccaagg acgttataca acggatgatg ggtatatctt caatgcatct
     6541 gatatcattg aggacacggg tgatgcttat atcgttcctc acggcgacca ttaccattac
     6601 attcctaaga atgagttatc agctagcgag ttagctgctg cagaagccta ttggaatggg
     6661 aagcagggat ctcgtccttc ttcaagttct agttataatg caaatccagc tcaaccaaga
     6721 ttgtcagaga accacaatct gactgtcact ccaacttatc atcaaaatca aggggaaaac
     6781 atttcaagcc ttttacgtga attgtatgct aaacccttat ca
//
LOCUS       DAVLWA010000090         6670 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLWA010000090 DAVLWA010000000
VERSION     DAVLWA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.090"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     regulatory      complement(47..135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(344..973)
                     /locus_tag="VYN80_001758"
     CDS             complement(344..973)
                     /locus_tag="VYN80_001758"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1541629.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(975..1757)
                     /gene="thiM"
                     /locus_tag="VYN80_001759"
     CDS             complement(975..1757)
                     /gene="thiM"
                     /locus_tag="VYN80_001759"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1541630.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(2039..2707)
                     /locus_tag="VYN80_001760"
     CDS             complement(2039..2707)
                     /locus_tag="VYN80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1541631.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2922..3010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3262..4398)
                     /gene="lctO"
                     /locus_tag="VYN80_001761"
     CDS             complement(3262..4398)
                     /gene="lctO"
                     /locus_tag="VYN80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1541632.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(5023..6513)
                     /gene="lysS"
                     /locus_tag="VYN80_001762"
     CDS             complement(5023..6513)
                     /gene="lysS"
                     /locus_tag="VYN80_001762"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1541633.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
BASE COUNT         1982 a         1430 c         1144 g         2114 t
ORIGIN      
        1 attagagttc agcttacaag attagacagt tcttttcgac atacgaaaaa aacatttcac
       61 atttcccttc gccagtctta actgtatcag gttcaatggg tatcatctca gcctaaagca
      121 ccccaaatgt ctttattatt taattatgtg attattataa cacacatttt atactagttc
      181 aagaaattga actggaaata cagccttgca ctcacaaaga cagcagatct ttcttttgca
      241 aaaaacaaat gacctgtttg atgaattagc cattcaagct gaatctggac atagcttttt
      301 aaaaaaggaa aatcctactt acttagaatc caaggataga tatctattgt tcactcattt
      361 cccgaacagt tttttctata ttttttgcat acgatattgc cgaaatgatt gaaacgccat
      421 ccatattggt ctttataatg tctttaatat gtttcgtctg tatcccacca attgcaacta
      481 aaggcatttg tggcaatagt tttctcatca attcaagacc ttcataacct atagcaccac
      541 cagcatcatc ttttgactgg gtatcaaata caggcccaac acctacataa tctacatatt
      601 caacttttga ttgttgaaat tcttcttcgt ttcttataga aagaccaatt attttatctg
      661 gcatcaattt tctaatttca tcaacaccaa tatcatcttg acctacatgt acgccatcgg
      721 cgtcaatttc cattgctaaa tctatatcgt cattaacgat aaatggaaca ttgtattttt
      781 tacaaagttc tttaatttgg atagctagct caagtttttc taagccttct aaagcaccct
      841 cacctttttc tcgaaattga aataaggtta taccaccttt taaggcttcc tcaacgactg
      901 tatatagatt ttttccttgg caagtagtcg ttccacaaat aaaatatagt tttagtaatt
      961 ctttatgaaa catcttactt cactcttttg aattccttta catcttcatc tgtaatctcg
     1021 tataaggcat ttataaattc aactttaaat gtcccaggaa gatgtccatt tggacgtttt
     1081 tctgccattt ctccagcgat attgtaaact aacattgctg tttctaatga ttttaattct
     1141 tgaccttttt ccagtccgat aaaacttgct actacagctc ctaataagca tcctgtccca
     1201 atgactttcg gcatcatagc actaccatta tgaatcgtta ccacttctcc attaacagca
     1261 atggcatcca cttcacctgt tactactatt ggaatattga acttctcatt tgctgctaga
     1321 gcaatttcgt caatattatc tacgcccgca ctatctactc ctttagatgc cacatctatt
     1381 cctactaaag aggcaatctc tccagcattc cccctaatcg cagctagttt gtaattgttg
     1441 attaaatcat ctgctacttt ttttctatat tctcctgctc cacaggctac aggatctaaa
     1501 actactggga cattatattt ctctgcaatt ttcagagcag cttggtataa tttccaattt
     1561 tcatctgtca atgttcctat gtttattaat aaaccaccag catactttaa caaatcctct
     1621 aaatctgctg gaaactcact catggctggt gaggcgccca gtgctactaa tccatttgct
     1681 gtgaaatttt ttactacatc attggttata caaatgacca atggtgcttt ttcttttaat
     1741 aattttaaac ttgtcatatt gaaatccttc cttttcactt tatacgatct actaatttcg
     1801 atttatcttt agttgagaat ttttttcatt tacattgaat gatttatact caatgaaaat
     1861 caaagagcaa actaggaggc taaccgcagg ttgctcaaaa cactgctttg agcctgtaga
     1921 taagactgac gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgtggt
     1981 ttgaagagat tttcgaagag tcttacctca tcaaatttgt aaatatcatg agccttctct
     2041 agacatcgta accaatatca aaaaaagcta attctaaagc gactgcttga ttccagcgtt
     2101 gctgaagttc tgtcaaatct tctcgatttt taccgacacg attgagttca tcaaccagaa
     2161 attgaaccca ctctgcaaag aaaggacctc tgtggagatt gatccattcc gaatgaatat
     2221 agacttcagg tagagccaaa tctttagaac cccagtctaa atagagacct tctgcaatga
     2281 ccagcataac caaaagatgg gcatagtctg atgaagccac tgcccaatac attagatcct
     2341 gaaaggcttt tgttacaggg tgcaaagtca cttctagata gtcattctct gctactttta
     2401 actctttaaa agccttttgg aaataaccat cttcatctgc ttcaagaaag cctagttgct
     2461 tggcaaaacg aagcttggat tcaagtttat ctgcgtgagc tacgcaggca cccagcatgg
     2521 ataagaaggc atcaaagaag tgataatctt gaatcagata gtcctttaag accttattct
     2581 caattgtccc cgcaaaaagt tccttaacaa aacgatgatt gattgcagcc tgccaatcct
     2641 tctgactgct ttttaataat tctccaacag tcaaacctgg ctgaaatgca tagtcttgtg
     2701 tttccatatt tacttctcct ctctttactt gttagtaatt aataaaacac caagaaatat
     2761 caagcaaaat cgtaattcca cttgatcctt ttaaagcaca tcgagagcat ttgcagagag
     2821 ctaactaaac aagcctatcc agtttatata aacaaaaaac tccaattaca atcaagaatt
     2881 agagttgact tacaagatta gaccgttcat ttcgccatac gaaaaaacgt ttcacatttc
     2941 ccttcgccag tcttaactgt atcaggttca atgggtatta tctcagccta aagcacccca
     3001 aatgtctcta ttatttaatt actgaaccag tatagcaaaa aatgaaagcc ctagcaagat
     3061 atttgaccga aaaatatctt tatatagtag attgaaacta gaatagtaca cctctacttc
     3121 taaaacattg ttagaaatcg atttgactgt cctgattgat ttgtcctatt cttatttcat
     3181 tttactatag ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt
     3241 cagtggaggc aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt
     3301 tgggttgtat ggattgtgac ggagcttgaa gtgtttgaca tcttcaatgg tctgagctcc
     3361 agacaattgc ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc
     3421 gacactacca ccgagagcca aaccatagat gacagggcgt ccaatagcaa ccaagtctgc
     3481 tcctgatgcc aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat
     3541 cggcacacgt ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc
     3601 accgtcgatt tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag
     3661 cgaacgttca acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta
     3721 ttcagcgata aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa
     3781 gtccattgat ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg
     3841 gaagacaaaa ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt
     3901 caagacaatc gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc
     3961 gtcatcctta ctaaagtaaa attgaaacca atgaggtgtc ccttgaaggg cttcagaaat
     4021 ctctggaagg tcaacagtag agtaagaact ggttgtataa agagaaccaa actcatgcac
     4081 accacgcgca gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc
     4141 cataatgatt ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc
     4201 aacatcgcaa agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct
     4261 taaagtgaaa gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac
     4321 ttgttgcgcc attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt
     4381 gcttgttttg tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac
     4441 acaactataa taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat
     4501 tttttcatac aatatactag ccctactttc cttaaactct aaactttagt ctatattaag
     4561 tgaatcgttg agattttttg ctgccaggca tttgaactac ttttttattt gctgattcta
     4621 tctactaata ctataaaaaa gcaagtgaat aagacaccag aacatcctct aataggacct
     4681 atggacttat atcagtccca tatatactgt ttaagcaaga tactatccta gttgacgcta
     4741 acttcctatc ccacttatta attttttatt tatagtgaat ctgaacaaat caattgggga
     4801 atccaagtca atttctagta ctttttgaac agtttcagcg tactatcgta aaatgaaata
     4861 aaacatgcac aaattgatta gggaatttaa accaatttct aacaatgtct tagaaatcaa
     4921 agtgtactat ttcaacttca atatactaca cttcattttc tccttaaatt cagtctcttt
     4981 ttgaatcaaa aaatcctctg gtaagagcca gaggataaga atttatttca ttgttgggaa
     5041 gagcaataca tcacggatag ttgttgtatc agtgaggagc atgcagagac ggtcgatacc
     5101 gattcccaaa ccacctgttg gtggcatacc gtattcaaga gcctcgatgt agtcatagtc
     5161 gatgcctgtc gcttcatcat caccaagttc tttagcttta gcttgggctt caaaacggct
     5221 aagttggtcg attgggtcgt tcaactcagt aaaggcatta ccgtactcct tagtcatgat
     5281 aaagagctcg aaacggtcag taaagcgttg atcttcagga tttttcttag cgagtggaga
     5341 tacagctact ggatgtccat agacaaaggt tggttggatt aaagtttctt caacaaactc
     5401 ttcaaagaag gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa ctggaacttt
     5461 cttctcagca gcgatagctt tagcttcttc taaagtcatg tcttgccaga aatcgacacc
     5521 agtaatttct ctgatagcat ccaccatatg aacacgctta aatggttcgt taatcttgat
     5581 ttcagtacct tggtagttga ctgggccatc gcccttaact gatttagcag cgtgttggat
     5641 aatgccttcc gtcaagtcca tgatgtcttg gaagtctgca taagcttggt aaacttcgat
     5701 agaagtaaac tcagggttat gagtagcgtc cattccttcg ttacggaaga tacggccaat
     5761 ttcatagaca cgttccatac cacccacgat aaggcgtttt aagtgaagct cagtcgcgat
     5821 acgaagcacc atgtcaatgt tttgggcatt gtggtgggtg ataaatggac gggcagcagc
     5881 accaccggct tcattatgaa gaacaggtgt ttccacttca aggaatcctt tttggtcaag
     5941 gtaacgacgg atttcagaga tgatttttga acgagtgaca aagcgttcaa agctttcacg
     6001 gttagaaatc aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg tcaaaccatg
     6061 gaatttctca ggaagaggac gaagagcctt agacaagtgt gtgatgtggg ttgccttgat
     6121 agagagttct cccatatccg tacgcatcac ttcaccttcg acaccaagga agtcaccaag
     6181 gtctgctttt ttgaagattt cgtagttttc ttcaccgaca gcatccttac gaacgtagat
     6241 ctgaatctgg ccttcgcggt cttgaaggtg ggcaaaacca acttttcctt taccacgttt
     6301 ggttaccaag cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt gttctttatc
     6361 gaggttggta tatttatctt ttaattcttg tgaatttgca gtacgttcaa aacgttttcc
     6421 gaaaggatcg attccttgtt cgcggagcgc agccattttt tcacggcgaa cgatctgctg
     6481 gtcatttagt tcttccatat gttctgtaga catgggatcc tcctagtttt actcaaaatt
     6541 gaatacgatg agtcattttt tgcgatactc ccattttatc ataaataagc aagaaattca
     6601 cggataatct ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa gaagtgaaag
     6661 tatctcccgc
//
LOCUS       DAVLWA010000091         6660 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLWA010000091 DAVLWA010000000
VERSION     DAVLWA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.091"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            110..310
                     /locus_tag="VYN80_001763"
     CDS             110..310
                     /locus_tag="VYN80_001763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1541634.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            345..1061
                     /gene="atpB"
                     /locus_tag="VYN80_001764"
     CDS             345..1061
                     /gene="atpB"
                     /locus_tag="VYN80_001764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1541635.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1075..1569
                     /gene="atpF"
                     /locus_tag="VYN80_001765"
     CDS             1075..1569
                     /gene="atpF"
                     /locus_tag="VYN80_001765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1541636.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1569..2105
                     /locus_tag="VYN80_001766"
     CDS             1569..2105
                     /locus_tag="VYN80_001766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1541637.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2120..3625
                     /locus_tag="VYN80_001767"
     CDS             2120..3625
                     /locus_tag="VYN80_001767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1541638.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3641..4519
                     /locus_tag="VYN80_001768"
     CDS             3641..4519
                     /locus_tag="VYN80_001768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1541639.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4605..6011
                     /gene="atpD"
                     /locus_tag="VYN80_001769"
     CDS             4605..6011
                     /gene="atpD"
                     /locus_tag="VYN80_001769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1541640.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6022..6441
                     /locus_tag="VYN80_001770"
     CDS             6022..6441
                     /locus_tag="VYN80_001770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1541641.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6513..>6660)
                     /locus_tag="VYN80_001771"
     CDS             complement(6513..>6660)
                     /locus_tag="VYN80_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1541642.1"
                     /translation="AKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
BASE COUNT         1977 a         1240 c         1471 g         1972 t
ORIGIN      
        1 aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac aaatttaaat
       61 tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta tgaatttaac
      121 atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa
      181 tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc gtagtttgat
      241 gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct tctcatttat
      301 tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa gaaagtatta
      361 atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc atgactttgt
      421 tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg accttgaaac
      481 ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga tttacagaac
      541 ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt ttattccttt
      601 tcatggtgat tgccaataac cttggcttaa tgacaaagct tcaaacgatc gatgggacta
      661 actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct tttcttgtca
      721 ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt
      781 ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca aacttcttat
      841 ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg agtttgttac
      901 ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct aatttggctt
      961 ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact cttttgacat
     1021 ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta actgatgcac
     1081 gtaacagtag gtgaattaat tggtaatttt attttaatca ctggctcttt tattcttttg
     1141 ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga agaaagagct
     1201 gaaaaaattg cttcagatat tgacagagct gaagaagctc gtcaaaaagc agaagtattg
     1261 gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac aatcattgaa
     1321 aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc taaactagaa
     1381 gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt agaagcttta
     1441 cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa aatcatctca
     1501 caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga tcagctagga
     1561 gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg ccttttgtcc
     1621 aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact caaatcaagc
     1681 aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta gacgagtcgg
     1741 ataaggaaaa aacaattgct tttttccaag attctgtgtc gcctttatta caaaacttta
     1801 tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt gtagattgct
     1861 tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct gctcatcctc
     1921 taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag
     1981 taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc atttttgcca
     2041 atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa gaaaatttga
     2101 aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt taattaagca
     2161 acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg taacctatat
     2221 cggggacggt atcgcgcgtg ctcatggcct tgaaaatgtc atgagtggag agttgttgaa
     2281 ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg ttggtattat
     2341 catcctaggt gactttacag atatccgtga aggcgataca atccgccgta cagggaaaat
     2401 catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc
     2461 agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag caccagctcc
     2521 tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga aagctattga
     2581 cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc gtcagacagg
     2641 gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata tgatctgtat
     2701 ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa cacttcgtca
     2761 gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac catctccatt
     2821 gctcttccta gctccttata ctggggttgc tatggcggaa gaatttatgt atcaaggtaa
     2881 gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc gtgaactatc
     2941 gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt tctatctcca
     3001 cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg gatcaattac
     3061 agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg caaccaacgt
     3121 gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg caggtattcg
     3181 tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc
     3241 catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg agttggaagc
     3301 ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga accgtggacg
     3361 tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg agaaacaagt
     3421 aaccattctt tatgctttga cacatggttt cttggatact gttccagtag atgatattgt
     3481 tcgtttcgag gaagagttcc atgccttctt tgatgctcaa catccagaga ttttggaaac
     3541 cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga ttacagagtt
     3601 tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtgt ctctaaatga
     3661 tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg ccatgcaaat
     3721 ggtatcggct gctaagttag gtcgctctga agaagctgct cgcaacttcc aagtttacgc
     3781 tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg gtgcttcaac
     3841 taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta tcacttcaga
     3901 ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg agttgaaaga
     3961 agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga tgggagctga
     4021 tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg cagaccaacc
     4081 tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc aaaatgaact
     4141 ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca gtcaaatgcg
     4201 tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaagcggatg aagagtacag
     4261 cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt tgcctcagtt
     4321 tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga atgctgcggg
     4381 tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg atttgacaat
     4441 tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa tcgtagcagg
     4501 tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt aggacctagt
     4561 tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt tcaggtaaaa
     4621 ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa aaacttcctg
     4681 agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg
     4741 aagtagcctt ggagttagga gatggtatgg ttcgtactat cgccatggaa tcaacagatg
     4801 ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta ccagtaggta
     4861 aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg gaagctcctt
     4921 ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt gatgagttgt
     4981 ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt gccccttacc
     5041 ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc
     5101 aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt actggtgttg
     5161 gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca ggcgttatcg
     5221 agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt atgcgtgttg
     5281 cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc
     5341 tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct gcccttttgg
     5401 gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg ggtcaattgc
     5461 aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct atctatgtgc
     5521 cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg gattcaacaa
     5581 caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac ccacttgctt
     5641 caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca gttgctgctg
     5701 aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct atccttggta
     5761 tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt atccagttct
     5821 tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt tcttatgttc
     5881 cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac gaccacttgc
     5941 cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa
     6001 tgggatttta agaggtgacc tatggctcag ttaactgtcc agatcgtgac accagatggt
     6061 ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg tgagatgggg
     6121 atcttgccac gacatgaaaa tatgattgcg gttttagcag ttgatgaagt aaaggtaaaa
     6181 cgtatcgatg ataaagatca cgtgaactgg attgcagtaa acggtggcgt tattgaaatt
     6241 gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga tatcgatatt
     6301 agccgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa
     6361 catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgccat taaccgtatt
     6421 aatgtcggaa atagactata agaaaaaatg aacttgaaaa taccaagttc attttttagg
     6481 ctctttgtca actgtagtgg gttgaaaaaa agctaagctc gagaaaggac aaattttgtc
     6541 ctttcttttt tgatattcag agcgataaaa atccgttttt tgaagttttc aaagttccga
     6601 aaaccaaagg cattgcgctt gataagtttg atgagattat tggtcgcttc caatttggcg
//
LOCUS       DAVLWA010000092         6610 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLWA010000092 DAVLWA010000000
VERSION     DAVLWA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.092"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            47..442
                     /locus_tag="VYN80_001772"
     CDS             47..442
                     /locus_tag="VYN80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1541643.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            453..824
                     /locus_tag="VYN80_001773"
     CDS             453..824
                     /locus_tag="VYN80_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1541644.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            834..3077
                     /locus_tag="VYN80_001774"
     CDS             834..3077
                     /locus_tag="VYN80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1541645.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3284..5059
                     /gene="spxB"
                     /locus_tag="VYN80_001775"
     CDS             3284..5059
                     /gene="spxB"
                     /locus_tag="VYN80_001775"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1541646.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5170..5517
                     /locus_tag="VYN80_001776"
     CDS             5170..5517
                     /locus_tag="VYN80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1541647.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(5634..6545)
                     /locus_tag="VYN80_001777"
                     /pseudo
     CDS             complement(5634..6545)
                     /locus_tag="VYN80_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1946 a         1262 c         1475 g         1927 t
ORIGIN      
        1 atactgttga gtgtaattga caaatgtaga ttttggaggt atggtaatgc agatttcaga
       61 tgcagaatgg caggtcatga agattatttg gatgcagggg gagcagacca gtacagattt
      121 gattagggtt ttggcagagc ggtttgactg gtccaagtcc acggttcaga cacttttggc
      181 tcgtctagtt gagaaagagt gtttgactcg gaaaaaagaa ggcaagtcct ttgtttattc
      241 agcgctttta actctagacc aaagtcggga tttacttgtc caagatatca aagacaaggt
      301 ttgttcccgt aggattagga atttgttggc tgacttgatt gtagaatgtg aatttactca
      361 gactgacttg gaagacttgg aagctgtgat ttcagagaag aaatcaagtg ctgtaacaga
      421 agtaagatgt aattgtatgt aaaggagacg tcatgttaaa tagtattgta accattattt
      481 gtattgccct tatcgcgttt atcttgtttt ggtttttcaa aaagcctgaa aaatctggac
      541 aaaaagccca gcaaaaaaac ggataccaag agattcgagt ggaagtcatg ggaggctata
      601 ctcctgagtt gattgtcctc aagaaatcag tgccagcccg cattgtcttt gaccgcaagg
      661 atccttcacc atgtctggat caaattgttt ttccagattt tggtgtacat gcgaacctgc
      721 caatggggga agagtatgta gtggaaatca cgcctgaaca ggctggagag tttagctttg
      781 cttgtggtat gaacatgatg cacggcaaga tgattgtaga gtaggtggag actatgacag
      841 aaattgtgaa agcaagctta gaaaatggca ttcaaaaaat ccgtatccaa gctgaaaaag
      901 gctatcatcc agcccatatc cagcttcaaa agggaattcc agctgagatt acctttcatc
      961 gtgctactcc ttcaaactgt tataaggaaa ttctgtttga agaagaaggt atcttggaac
     1021 caatcggcgt agatgaggag aaagtcattc gttttacacc tcaagaatta gggcgacatg
     1081 aattttcttg tggcatgaag atgcaaaagg gaagctatac agtcgttgag aagactcgaa
     1141 aatctctatc tctcctgcaa cgtttttgga ttactagtat ctgtactgtg cctcttgtga
     1201 ttctcatgat tgggatgttg gcaggtagca ttagtcatca agtcatgcat tggggaacct
     1261 ttctagcaac aacgcctatt atgttagttg caggtaagcc atatatccag agtgcttggg
     1321 ccagttttaa aaagcacaat gccaacatgg ataccttggt tgcgctggga actctagtgg
     1381 cttatttcta tagcctagtt gctctctttg ctggtctccc tgtttacttc gaaagtgctg
     1441 gatttatcct ctttttcgtt cttttgggag cagtttttga ggaaaaaatg agaaaaaata
     1501 cgtcccaagc tgtggagaaa ttactggact tgcaagctaa aaccgcagaa gtcttgagtg
     1561 atgatagtta tgtccaagtt cctttggaac aagtcaaggt aggcgacctg attcgagtgc
     1621 gtcccggtga aaagattgct gttgatggtg tcgtagtaga aggtgtctct agtattgacg
     1681 aatccatggt gacaggtgag agtctgcctg tggacaagac agttggagat actgtcattg
     1741 gctcaaccat caatcatagt ggaacgcttg tctttagagc agaaaaagtt ggctcagaga
     1801 ctgttttggc tcagattgtg gattttgtga agaaagctca gacaagtcgt gcgccgattc
     1861 aggacttgac ggataagatt tcagggattt ttgtcccagt agttgtcatt ttaggaatca
     1921 tgaccttttg ggtttggttc gtcttgctca gggatagtgt ggttgtgctt ggagctagct
     1981 ttgtgtcctc tcttctctac ggagtggcgg ttttgattat cgcctgtcct tgtgccttgg
     2041 gacttgcaac accgacagcc cttatggtgg ggacaggacg tagtgccaag atgggggttc
     2101 tcctcaaaaa tggaactgtc ttacaggaaa tccagaaagt tcaaaccctt gtctttgata
     2161 agaccgggac tttgacggaa gggaaacctg tggtaacaga tgtcatcggc gacgaagtag
     2221 aagtgtttgg attggcagcc tccttggaag atgcttctca acacccactg gctgaggcta
     2281 tcgttaagcg agcgagtgaa gctggacttg agtttcaaac tgttgaaaat tttcaggcct
     2341 tgcacgggaa aggtgtttca gggcgaatca atggaaaaca agttttactt ggaaatgcta
     2401 aaatgctgga tggcatggat atttctaata cttatcaaga taaactagaa gaactagaaa
     2461 aagaagctaa gacagttgtg ttcttagctg ttgacaatga aatcaaaggc ttgcttgctt
     2521 tgcaagatat tcctaagaaa aatgctaagc tagccatcag tcagctaaaa aaacgtggtc
     2581 tcgggacagt catgctgaca ggagacaatg ctggtgtggc gcgtgctatt gcagatcaaa
     2641 tcggaattga agaggtcatt gcaggcgtct tgccagaaga aaaagcccat gaaatccata
     2701 aactgcaaca gtcaggtaaa gtagcctttg ttggtgatgg gatcaatgac gctcctgccc
     2761 ttagtgtagc agatgtgggg attgctatgg gagctggaac agatatcgcc atcgagtcag
     2821 cagatttggt gttgacaacc aataatcttt taggagtggt gcgtgccttc gacatgagta
     2881 agaaaacctt tcatcgtatt ctgctcaatc ttttctgggc ttttatctac aatgttgtcg
     2941 gaattccgat tgcagcagga gtcttttcag gtgttggact ggctctcaac ccagaattgg
     3001 caggtctagc catggccttt agttctgtat ctgttctgac cagttccctt ctgttaaatt
     3061 ttagtaaaat agactaaaag taatgactag atttttttgt tataaaacag aaatgacgac
     3121 attttcaaaa ctgtattaga atagagaata gagagttttg agcagatttt tagaaaagtc
     3181 agcataaata tgatacagtg gaatagtaaa aatttggaga acgtttccaa ttctatgtaa
     3241 tcgtattctc caagtctaaa aaaattgaag gagagttatc attatgactc aagggaaaat
     3301 tactgcatct gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata caatctacgg
     3361 tatcccatca ggaacactca gctcattgat ggacgctttg gctgaagaca aagatatccg
     3421 cttcttacaa gttcgccacg aagagacagg tgctcttgca gcggttatgc aagctaaatt
     3481 cggcggctca atcggggttg cagttggttc aggtggtcca ggtgcgactc acttgattaa
     3541 cggtgtttac gatgcagcta tggataacac tccattccta gcaatccttg gatcacgtcc
     3601 agttaacgaa ttgaacatgg atgctttcca agagcttaac caaaacccaa tgtacaacgg
     3661 tatcgctgtt tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag taattgacga
     3721 agcctgccgt gctgcagttt ctaaaaaagg tccagctgtt gttgaaattc cagtaaactt
     3781 cggtttccaa gaaatcgacg aaaactcata ctacggttca ggttcatacg aacgctcatt
     3841 catcgctcct gctttgaacg aagttgaaat cgacaaagct gttgaaatct tgaacaatgc
     3901 tgaacgccca gttatctatg ctggatttgg tggtgttaaa gctggtgaag tgattactga
     3961 attgtcacgt aaaatcaaag caccaatcat cacaactggt aaaaactttg aagctttcga
     4021 atggaactat gaaggtttga caggttctgc ttatcgtgtt ggttggaaac cagccaacga
     4081 agtggtcttt gaagcagaca cagttctttt ccttggttca aacttcccat ttgctgaagt
     4141 ttacgaagca ttcaagaaca ctgaaaaatt catccaagtt gatatcgacc cttacaaact
     4201 tggtaaacgt catgcccttg acgcttcaat ccttggtgat gctggtcaag cagctaaagc
     4261 tatccttgac aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa acgttaagaa
     4321 caaccaaaac tggcgtgatt acatgaacaa actcgaaggt aaaactgagg gtgaattgca
     4381 attgtatcaa gtttacaatg caatcaacaa acatgctgat caagacgcta tctactcaat
     4441 cgacgtaggt aacactactc aaacatctac tcgtcacctt cacatgacac ctaagaacat
     4501 gtggcgtaca tctccactct ttgcgacaat gggtattgcc cttcctggtg gtatcgctgc
     4561 taagaaagac aatccagatc gccaagtatg gaacatcatg ggtgacggag cattcaacat
     4621 gtgctaccca gacgttatca caaacgttca atacgacctt ccagttatca accttgtctt
     4681 ctcaaatgct gagtacggct tcatcaagaa caaatacgaa gatacaaaca aacacttgtt
     4741 tggtgtagac ttcacaaacg ctgactacgc taaaattgcg gaagctcaag gagctgttgg
     4801 attcacagtt gaccgtatcg aagacatcga tgcagttgtt gcagaagctg ttaaattgaa
     4861 caaagaaggt aaaactgttg tcatcgatgc tcgcatcact caacaccgtc cacttccagt
     4921 agaagtactt gaattggatc caaaacttca ctcagaagaa gctatcaaag ccttcaagga
     4981 aaaatacgaa gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag aaggattgca
     5041 atcacgcgca attaaataat tcctctcgcc gaaaatcaaa tatgaaactt gtagacttta
     5101 ttctttgatt ttcatagagc agaactttaa aagatcggag caatccggtc tttttatgga
     5161 ggagagtaaa tgaatttaaa tcaattagat attatcgttt caaatgttcc ccaagtctgt
     5221 gctgacttgg agcatatttt ggataaaaag gcagattatg ctgatgatgg ttttgctcag
     5281 tttacgattg gtagtcactg ccttatgttg tcacaaaatc atttggttcc tttggaaaac
     5341 tttcagtcag gaatcattat tcatatcgag gttgaggatg tagaccagaa ctacaaacgg
     5401 ttgaacgagc ttggtataaa ggttttacac ggaccaactg taaccgattg gggaacagag
     5461 tccttattag ttcaaggacc tgctggtcta gtgcttgatt tttatcgtat gaaataggtc
     5521 gcgtagtcat ttgacgttaa ccttagctat ttttaaaata gaaattgaga atatgaacta
     5581 ttgaaagatc ggagcaatcc ggtcttttta tataaagaaa ttgcatataa tactcatatg
     5641 ctttcaaaaa gcattctagt ccatctccga ttaacgatgg actttatcac ctccttctcc
     5701 agtccttgta tgacatcttg aagttgattc atgacatctt ccaaagttcg aaaggcctta
     5761 ttcttaaatc cacgtttacg aatctctttc cacacttgtt caatgggttc atctctggtg
     5821 tgtatggagg aataaaggta aaaccaatat tagtcggaat ctttaaggta cttgatttat
     5881 gccatatagc attgtccata acgagtaaaa gataatcatc tggataagct tgtgaaagct
     5941 cttctaaaaa ggcgttcatc cactcagtat tacatccacc agctattaag aaaaatgatt
     6001 cgcctgtatg ggcatcaaca gctccataac aatagtgaaa ttctcgtata tagtgactat
     6061 ggacatgtgg acctactcct attggagccc aacaagatcc cagtttactg attctaccga
     6121 aaccagcctc atcttggtac atcaagcgaa ccttacgaaa acgtctactg cttttaaagc
     6181 gctttctttt cttcttgaat tgagatttta tttttagacg cgacaatggt ttgagcgtct
     6241 gctttcctag gatgttctgg acgtggcata atatttcgcc aaccatggtg cttcaacagt
     6301 tgatagaagg catcacgtgt gtaggaacga cctaactcct ttttataagc ctgaaataag
     6361 gcatcaattg taacaaattc tcctgcctct gtagccttca aatggcgggc aagaaagact
     6421 ttcttttcct caatcgtcat atatgcatgg ttacggccac cacgtgtttc ttgaaggaga
     6481 gagtcgagtt cgaactcctc atattttttt acatttcgcc aaatcgttgt ttgattacag
     6541 tctatagtgt attgaatcta taacagtaca ccttagctgc taaaatattt ctataaatta
     6601 gtttgacttt
//
LOCUS       DAVLWA010000093         6603 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLWA010000093 DAVLWA010000000
VERSION     DAVLWA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.093"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(48..1370)
                     /locus_tag="VYN80_001778"
     CDS             complement(48..1370)
                     /locus_tag="VYN80_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1541648.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVVLLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1461..1549)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1753..3315)
                     /locus_tag="VYN80_001779"
     CDS             complement(1753..3315)
                     /locus_tag="VYN80_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541649.1"
                     /translation="MKKFLLATTLLLTLVLGGCQYLPWNKSAETPSDSTSETSVATPE
                     IDYSSYNKVLDDYEKVAKGTLPTGMDVNPLASQVKSSQGFYTDVVYHKTDLNNDGVDE
                     LLLALEMKSGEKSLLDIRTLKDEKVIRLTNQENRLDQIGERMTVGILPDNSLLYRGAG
                     TATSHIYAHYQFSEDGQSLVKDKEAQELADLGVGSPISLETLSWKSVSDKLPGGSSAD
                     KGKPSVDYSPYNSILKDYEFILDYKSEVKDNINPTANIYAGMAERSQAFTYAMVDMDK
                     NGIDELIVSSKNGSEILDIFTLKDQKVIRLTNAENKMQMLGTRFKAYLLENGNLYTQW
                     NPFAGDPQTIHTIWKLNSSGDKLEKVKEAKTEDEVSPGTRMNLSSLTWKSVTEKFAAT
                     ASSTSKTASGKMDINAIKNGDFSSIEGRWSNGHGGEDLVFSKDGLIAPEKFKVDLTES
                     EFVNGFLRARYSAPDFGIFIQFVPAGVRIPDGTSRGELVTDASDISKDRIIFHAAFPL
                     HGDPRQFLYRVE"
     gene            complement(3456..4470)
                     /locus_tag="VYN80_001780"
                     /pseudo
     CDS             complement(3456..4470)
                     /locus_tag="VYN80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
     gene            complement(4529..5917)
                     /locus_tag="VYN80_001781"
     CDS             complement(4529..5917)
                     /locus_tag="VYN80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1541650.1"
                     /translation="MKSKTVLVTSLAAVTFGLVSLVDTAGALPFSAKTVQADTQTLTT
                     AAKYYILVPKDSEVFKNWRGTANRPIIWATKTTPELSYFQESARNYIANENKSGANYI
                     EWKGTVEEFKEAIKNLTDKKLTPNPAPIPQSDKKPTPDPTPNPQSTVPSGWSGSSYYQ
                     NGTKVTSKWIFDKNYNSYFYLDASGNYVQNAWEGDYYLKSGGYMAKKEWIYDKNYGAY
                     YYLTPDGYYARNTWVGNYYLKSNGKMAKSEWIYDKNYSSYYYLTSEGSYARNTWVGDY
                     YLKSNGKMAKGEWIYDNNYSSYYYLTSEGSYARNTWVGDYYLKSNGKMAVSERTPDGY
                     QVDGSGKWISSEGTHTASSSQATSLGISLYNVNDAMTYIDIALPSTPSSSYQVAYDFA
                     LVDANTGSVSTQTQFTVTYNPSLRVFQTRHFFSATPDGSYRFVIKGKGQDGKVYDGQS
                     VVFRVKNHRFVG"
     gene            complement(6093..>6603)
                     /locus_tag="VYN80_001782"
     CDS             complement(6093..>6603)
                     /locus_tag="VYN80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541651.1"
                     /translation="CLIKIKNYKFYMIVLNRIPLHTFMVYFSSVLLWIMYLTKPVDVS
                     NALPLPIILPLEKINPFYMILGYWLLNKRCEYQLHQYLYPVMRGSSHDGRIYALPEEL
                     FIWNRNRSQKIGKWKRDRRYNTTTDLVEAQIGYSKFPFFLLATKWSVDPYDQNWTIGE
                     KAYLTSRWK"
BASE COUNT         1932 a         1401 c         1198 g         2072 t
ORIGIN      
        1 taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta tttgtcaaaa
       61 tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat caagtttggc
      121 agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa ctcgataaat
      181 cctcctaaca agaccatgag cacaaaaacc acacggtaga gccagataaa gcgaacccca
      241 aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt tgtaaaggca
      301 aaaagtacaa ggaagatggt caagagagca ggcccaaagt gtgaaaagac tgttgagaaa
      361 gctgactgag tcaaggcaac cccattcaag tcaccgctcc aaactccagt taccaagatg
      421 gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt catggaaatc
      481 aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg agcagaaccc
      541 agacccgatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc catccgaacg
      601 ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt aaagactaaa
      661 gcgattgtgc cagggatttt tccgatatta aagaaaataa ctgtaagagt tcctaagata
      721 taaatgaggg ccataaaagg aacaacagta gttgaaacct tagaaataga cttgagtcca
      781 ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatggc tggcgaaatc
      841 gtcgttgtat tttggataga ttctgcaatc gagttgactt gggtgaaggt tccgattccc
      901 aagagaacaa ccaatactcc tgctactgca aacaaaacag caagtggtcg ccacttttct
      961 cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg gtcgtccttg
     1021 gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc aaagaaagcc
     1081 gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt cgcaactcct
     1141 atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc aaaactggat
     1201 acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg cagacgcaaa
     1261 acctgcaaga gtcctagccg catggttagg taaatccctg ttccgaccaa taaaatcaag
     1321 aggggcggtc cccaagcaaa agcatcgatt gatttaagca attctaacat ttccttctcc
     1381 tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg gaatgcttag
     1441 ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt ttgagcagtt
     1501 gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca tttacagtca
     1561 tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt atttaattga
     1621 ttcttatttt acaaaaaatg ttggcttttg tcaatgtgtt ttggaaaaat cttatttgac
     1681 tctgtagaag aaggattgag cattcccatg tgcgtggggt gctattagtt attaaatcaa
     1741 gctgtttcac tcttattcca cacgatataa gaattgtctt ggatcaccat gtagcggaaa
     1801 tgcagcatgg aagatgattc gatctttact aatatccgaa gcatccgtga ccaactcacc
     1861 cctagatgtc ccatccggta ttctaacacc tgctggtacg aactgaataa agattccaaa
     1921 gtctggtgct gaataacgag ctcgtaaaaa accatttaca aattctgatt ctgtaaggtc
     1981 aactttaaat ttctctggtg ctatcaagcc atctttacta aagactaagt cttctcctcc
     2041 atgaccatta ctccatcttc cctcaatact agaaaaatct ccatttttga tggcgtttat
     2101 atccattttc cctgaagctg ttttgctagt tgaagatgct gttgcagcaa atttttctgt
     2161 tacagatttc caggtcagac tagatagatt cattcgagta cctggactga cttcatcctc
     2221 tgtcttagct tctttaacct tctctagttt gtccccacta ctattcaatt tccaaatggt
     2281 atgaatggtc tgtggatctc ctgcgaacgg attccactgg gtatagagat taccattttc
     2341 taatagatag gccttgaaac gagttccaag catctgcatt ttattttcag cattggtgag
     2401 gcgaataact ttctgatctt tgagtgtaaa aatatctaaa atctcactac catttttcga
     2461 agagacaatc aattcatcaa tgccattttt gtccatatca accattgcat aggtaaaggc
     2521 ttgactgcgt tctgccattc ctgcatagat attagcagtt ggattgatgt tatccttaac
     2581 ctctgactta tagtctaaaa taaattcata atccttcaaa atgctattat aaggactgta
     2641 atctacactt ggtttcccct tatctgcact gcttcctcct ggaagtttat ctgaaactga
     2701 cttccagctt aaggtctcta gagaaatggg cgaaccgacc ccaagatctg ctagttcttg
     2761 agcttcctta tcctttacca aagactgccc atcttcacta aattggtagt gagcatagat
     2821 gtgactcgtt gctgtaccag ctccacgata gagcagagaa ttgtctggaa ggataccgac
     2881 cgtcattctc tcaccaatct ggtctaaacg attttcttga ttggtcaaac gaatcacctt
     2941 ttcgtctttc agcgtacgaa tatcaagtaa actcttttcg ccactcttca tttcaagagc
     3001 aagtaaaagt tcatcgactc catcattatt caaatccgtc ttgtggtaaa ccacatctgt
     3061 ataaaagcct tgagaagact tcacttgact agccagagga ttgacatcca tacctgtcgg
     3121 caaggtgcct ttcgctactt tttcatagtc atccaaaact ttattatagg aactgtagtc
     3181 tatctctgga gtcgctacag atgtttctga tgttgaatca gatggtgttt ctgcgctttt
     3241 gttccaaggt aaatactgac agcctcctag aactagtgtc agtaaaagtg ttgtggctaa
     3301 taaaaacttt ttcatattac tcctttatct aagagaaagg aaccagcctg tgccaattcc
     3361 tttcgctatt tttattgtat caaaatgttg cctataaaca aattattttc cgtttctatt
     3421 tatttgttat aagccatttc tcacaagcta tcattctagc gaacccactt acctgagccg
     3481 tctactttgt agccatctgg ggtacgttcg ttgactgcca tcttaccatc tgatttgagg
     3541 tagtagtcac ctacccatgt attacgggcg taacttcctt ctgatgttag gtaatagtaa
     3601 gattggtagc tactatcata gatccactca ctcttagcca ttttaccatc tgatttgagg
     3661 tagtagcttc cagaccaagc attacgtgca tagctaccct ctgctgtcaa gtaatagtat
     3721 gattgatagt tattgtcata aatccattcg ctcttagcca tgtagccacc tgacttgagg
     3781 tagtaacttc cagaccaagc attttgaaca aagttacctg aagcattgag gtagaagtat
     3841 gaattgtact gaacatcaaa aatccattga ttgactactt tcacaccagc tttgtagtaa
     3901 gaagaacctg accagccagt ctgacctgag ctagagttag tagctggttt tgaagtttca
     3961 gtagctgttg gtttttccgt ttctttagga gttgaagagt ctagaggatt gacatatttt
     4021 tgatctttaa cagtgaatgt cgctgatttg ccaataaatt tatacaaaac acttctgtaa
     4081 tcgtcttctc catgaaattg taagtaatat tcaccgtctg gaacatttgc aaaattagtc
     4141 caaccttgct ttgtaatcgg aacagatgat ctatcgaacc tttcattgaa agtatctttt
     4201 ctaggaattt gccctgtttt tgcatttaca atatctgcat atatatacat gatagtgtac
     4261 aggtacattt tcaacagtca tatccacata gatacttcca ttttctgatc ctggtgtaat
     4321 cttagatttt acctcatgat tctcggtacg agtaatttcc gcatgcactg tttgtgctga
     4381 aaaagggaga actcctgctg tgtcgattgt tgatactact ccgactgttg aaagtgccac
     4441 agcacatact aataatttct ttttgttcat gttattcatt tcctttattt tatttttaat
     4501 atttatttgt ttaaaaccta tcactcatct atccgacaaa acgatgattc tttacacgaa
     4561 agacaactga ttggccatcg tataccttgc catcttgtcc ttttcctttg ataacaaagc
     4621 gataactacc atctggagtg gcactaaaga agtgacgagt ttgaaagaca cgtaagcttg
     4681 gattgtaggt cacagtaaac tgggtttgtg tagaaactga acctgtattt gcatcaacta
     4741 aggcaaaatc ataggctact tgataagatg aggatggagt gctaggtaga gcgatatcaa
     4801 tataggtcat agcatcattg acattgtaaa ggcttattcc taatgatgtt gcttgtgaac
     4861 tgcttgctgt atgagttccc tcagaactaa tccacttgcc tgatccatcc acttggtagc
     4921 catctggtgt acgttcgcta actgccatct ttccatttga cttgagataa taatcaccta
     4981 cccatgtatt acgagcataa cttccttccg atgttaagta atagtaagag ctgtagttat
     5041 tgtcataaat ccactcacct ttagccatct ttccatttga cttgagataa taatcaccta
     5101 cccaagtatt acgggcgtaa cttccttccg acgttaggta gtaatatgaa ctgtagttct
     5161 tatcatagat ccactcgctt ttagccatct taccgtttga cttgagataa tagttgccca
     5221 cccaagtatt gcgagcatag tagccatctg gagttaagta ataataagct ccatagttct
     5281 tatcatagat ccactccttc ttagccatgt agccacctga cttaagatag taatcaccct
     5341 cccatgcatt ttgaacataa ttacctgaag catctaggta gaagtaggaa ttatagttct
     5401 tatcgaaaat ccacttacta gtgactttag taccattttg gtagtaagat gatcctgacc
     5461 aaccagacgg aactgtagat tgtggatttg gagttggatc tggggttggt tttttatccg
     5521 attgtgggat tggagctgga tttggagtta gttttttatc cgtcagattc ttaatcgcct
     5581 ctttaaactc ttctacagtt cccttccact ctatgtaatt ggcaccagac ttattctcat
     5641 tagcaatata gttgcgagca gattcttgga aataagataa ttctggagtg gtttttgtgg
     5701 cccaaataat gggacgatta gcagtacctc tccaattttt aaatacttct gaatccttcg
     5761 gaacaagaat ataatatttt gcagctgtag tcagcgtttg tgtgtcagct tgtaccgttt
     5821 ttgctgaaaa aggtagagct cctgctgtat ctacaagaga cacaagacca aatgttacag
     5881 cggctaggct ggttactaaa actgtttttg acttcattat caaaacctcc tttaaatttt
     5941 tattatttag gcttacagta agggatggac tctactatat ttatgaaaag agtatctgtg
     6001 tttatacttt atttgattag caaatgaaat ttttatgaaa aaagtatact taatcttttt
     6061 cctagttatt agactaaaga ctttctattt tatcatttcc acctacttgt caaatatgcc
     6121 ttttctccta ttgtccaatt ttgatcataa ggatccactg accattttgt tgctagcaaa
     6181 aaaaacggaa acttactata accaatttga gcttcgacta gatcggtggt cgtattgtac
     6241 cttctatctc gtttccactt accaattttt tgacttctat tcctattcca gataaaaagt
     6301 tcctctggta gtgcataaat tctaccgtca tgagaactcc ctctcattac aggataaagg
     6361 tactgatgca actgatattc acatcgctta tttaagagcc aatatcctag aatcatatag
     6421 aagggattta tcttttcaag aggtaagata attggcaaag gtaaagcatt actcacatcc
     6481 actggttttg taagatacat tatccataac aatactgatg aaaaataaac cataaatgta
     6541 tgtagtggta ttctattcaa aacaatcata tagaatttgt aattctttat ctttatcaaa
     6601 cag
//
LOCUS       DAVLWA010000094         6557 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLWA010000094 DAVLWA010000000
VERSION     DAVLWA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.094"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     regulatory      52..150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            234..1025
                     /gene="thiD"
                     /locus_tag="VYN80_001783"
     CDS             234..1025
                     /gene="thiD"
                     /locus_tag="VYN80_001783"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1541652.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1235..1867)
                     /locus_tag="VYN80_001784"
     CDS             complement(1235..1867)
                     /locus_tag="VYN80_001784"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1541653.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1860..2663)
                     /locus_tag="VYN80_001785"
     CDS             complement(1860..2663)
                     /locus_tag="VYN80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1541654.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2663..3187)
                     /gene="thiW"
                     /locus_tag="VYN80_001786"
     CDS             complement(2663..3187)
                     /gene="thiW"
                     /locus_tag="VYN80_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1541655.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTSIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSATKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(3192..3884)
                     /gene="tenA"
                     /locus_tag="VYN80_001787"
     CDS             complement(3192..3884)
                     /gene="tenA"
                     /locus_tag="VYN80_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1541656.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3895..4545)
                     /locus_tag="VYN80_001788"
     CDS             complement(3895..4545)
                     /locus_tag="VYN80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1541657.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4547..5931)
                     /locus_tag="VYN80_001789"
                     /pseudo
     CDS             complement(4547..5931)
                     /locus_tag="VYN80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(5932..6492)
                     /locus_tag="VYN80_001790"
     CDS             complement(5932..6492)
                     /locus_tag="VYN80_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1541658.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2027 a         1438 c         1303 g         1789 t
ORIGIN      
        1 caaatattac tatataaaca ataaaaatat gctatactaa agaaaaaaga aaacaaccac
       61 taggggtgcg taaagctgag attaacgact gttagatccc tctgactcaa tctaggtaat
      121 gctagctgat ggaagtggaa atgataatgg ggactagcag tcttctattg cctttctaaa
      181 acagactagc ttgttcttaa gaatacaaac ttcagttggt tgggaggttt tagatgactt
      241 atttacccgt tgctttgacc attgcaggga ctgaccctag tggtggtgct ggcattatgg
      301 cagatttaaa gtcattccaa gcgagagatg tctatggaat ggctgttgta accagtcttg
      361 tcgctcaaaa taccagaggt gttcagctaa tcgagcacgt ttctcctcaa atgttgaaag
      421 cccaattgga gagtgtcttt tctgatattc cacctcaggc tgtaaaaact ggaatgttgg
      481 ctactactga aatcatggaa atcatccaac cctatcttaa aaaactggat tgtccctatg
      541 tccttgatcc tgttatggtt gctacaagtg gagatgcctt gattgactca agtgctagag
      601 actatctcaa aacaaactta ctacctctag caactattat tacgccaaat cttcctgaag
      661 cagaagagat tgttggtttt tcaatccatg accccgaaga catgcagcgt gctggtcgcc
      721 tgattttaaa agaatttggt cctcagtctg tggttatcaa aggcggacat ctcaaaggtg
      781 gtgctaaaga tttcctcttt accaagaatg aacaatttgt ctgggaaagc ccacgaattc
      841 aaacctgtca cacccatggt actggatgta cctttgctgc agtgattact gctgaactag
      901 ccaagggcaa gagtctttac caggcagttg ataaggccaa ggcctttatc acaaaagcta
      961 ttcaagatgc ccctcaactc ggtcatggtt ctggtccagt caaccataca acttttaaag
     1021 attaagaaaa aaaactctct agttcccact ttaagggaat tagagagttt ttatactctt
     1081 cgaaaatctc ttcaaactac gtcagcttcc atctgcaact tcaaaacact gttttgagct
     1141 gacttcgtca gtcttatcta aaacctcaag gcagtacttt gagcaacctg cgactagctt
     1201 tctagtttac tctttgattt tcattgagta ttaattagga aagaatgtca tgcaactttg
     1261 ttaaaaaggc ttgcgttttt gcctcaatat cttctgcttg catcaaatca cgtacaacag
     1321 ctacaccagc tatgccagtg cccataagct gatcaatatt ctccgaagtc aagccaccaa
     1381 tagcaactat tggaatggca accgtttggc aaattgtttt caaggttgaa atcagggtga
     1441 tgggtgcatt ttccttggtg gttgtcggaa aaatggctcc tgtccccaag taatctgcac
     1501 ctgatttctc cgcttccaga gctcttttaa ccgttttagc ggtgacaccg aggatttttt
     1561 caggacccaa gacttgtcgg gcaaccgaaa ctgggagttc gtcatctcca atatgcagac
     1621 cagcagcatc aaccgcaaga caaacatcca accgatcatc gattatcaag ggtacctgat
     1681 aagcatctgt tatttccttg acttgttttg ccagttgata atattgattg gttgtgagat
     1741 ttttttctcg caattggact atggtaaccc ctgaacggca ggccgtctca acttttgcaa
     1801 gaaagctttc cacggaatct tgatagcgat tggttaccag atatagtcta agtgcttctc
     1861 tattcataaa cctctccttt gatggtatct agccaatttt catctcttct taggagcgaa
     1921 agctgattga gtacttggta acgaaattct tccaatccca ttccttgaac aactattttc
     1981 tcagcagcga tattgagata agagactgct aagcaagaag cttcaaaacc agtctttcct
     2041 tggctgagaa aaacggctgt taaggctcca accaaatctc ctgtccctgt tatccagtct
     2101 aattctgcac agccatttcc cagtacagcg acctgatttt tcgaaacgac gaggtccttg
     2161 ggacctgtga ctaagaaaga cataccatga taggtctgac accagtcttt caagacttga
     2221 agcaaatcct ccgtttcttg atctttagca ctcgcatcga ccccaacgcc gtggtgcttt
     2281 aatccaacaa gacttcgaat ttctgacatg tttcctttaa ggaccgtagg tctatagtct
     2341 aaaaggtctc tgactaagct cttacgaatg gatgaagctg ttacgccaac cgcatctact
     2401 accattggga gacaaacttg agctgcataa gaagctgcta tacggattgc tttctccttc
     2461 tcagctgaca aatgccccaa attgatgaag agagcctgac tttgcttagt aaaatcaaga
     2521 acctcacggg gatcatctgc catgacaggt ttgcatccca gagccaaaat cccatttgcc
     2581 agcatctcac aagaaatctc attggtcata cagtgaatga gggaactaga gcctatagga
     2641 aagggatttg taaattcctg catcagtcta tcctttcact aaagaaatat ccctgcactt
     2701 ttttaaagaa ttcctgcttg attaaaaatc gaaaggcaat aaaggaaatc gctgtaccaa
     2761 tcaaggttgc tccgaaaaat cgaggcgtgt agataaacca gctaagctta gtagctgatc
     2821 ctgtaaagag taccataaca ggataggaaa caatagaacc aataatacct gttcccacaa
     2881 tttctcccaa ggcagaaaag taaaattttc gaccgtactt ataaaagaga cctgctagaa
     2941 gggctccaaa agtcgctcct gtgagagcta aaggcggaat cccttgagtc gtcatacgga
     3001 taaaggctgt cactgtagcc atagccaagg cataaacagg tcccatcatg attcccgcta
     3061 gaatattgac tacactggac atcggtgcca ttccctcaat ccgaaagata gatgtaagga
     3121 ctacatcaag ggcaatcatc atagataaaa tggtcaattt gtgaacttgt agttggtgct
     3181 ttctcatgtt tctattcttc tcctttttct aaagactgta aatcgctctt ccatgtctgg
     3241 tgttggtaag ccatctccca aaacttggct tccatatgaa cactgatgtg gaaggcatct
     3301 agcatttttt gcttatctgt ctcatcactt tctcgataga gctgattgac cagtgctccc
     3361 tcctctctga tctgttgctc taactcatcc gtaatataag tttcaatcca ttgttgatag
     3421 agaggatttg gtgatggttt aagattaagt gatttgccta tatcatggta taaccaagga
     3481 caaggaagca agcttgcaaa agcgatggct aagttcggtt ctgcaaattg cctataaata
     3541 tgagaaatgt aatgataaca ggttggagcg attggatgtt gctccatttc ctggtcgctg
     3601 atttccaatt ccttgaaaaa ttgttggcga ataaataact caccctccac taaaccctga
     3661 gcattttgtt tcaagagtct tttcatctct tggtttgaag tcttatcagc caaaagatga
     3721 tagatttctg agaaagcctt cagatagtag gcatcctgaa tcaggtaata gcggaaaatg
     3781 gcaggttcta aattcccctc ttgtaattgt aaaataaagg gatgatgaaa ggaagcctgc
     3841 caagctttct tggataattc catcgcaata tctgtaaatt ccataataac tcctttataa
     3901 aaatagactg gtttgaagca ataaaaagaa aagcaggtag attaattttg tttttttagg
     3961 aatataaaaa gtccgatagc tattcttcaa ctgtgcatgt tcgtcatatc cgtgagcaga
     4021 tagagctctc aggtaaagat ggcgccacct aaagactgtc atcagaacct tactgtaaat
     4081 caagggcgac caaaaatgta gttcttgacc acgtaatagg caagcttctt tgagggactt
     4141 gatttcttgc tgaatgagag gaaaggaatt gaataccaca atcaaggcat aggaccaaga
     4201 gcgtgatagc cccttttgag ccaagtacaa gagaagctct tttagtgaaa tagaggaaac
     4261 aaagacaagg ccgatacaaa ctgtcacaaa ggccctcgtt ccaagcatga ctgcctgtga
     4321 agcatctccg tgtaactgaa ctgcccagta gttggcaaaa gatggtaaaa tggcaagtat
     4381 gatcatccaa gctaacattt taaatcgacg gtaatagagc ataaagagaa tacaaaatgc
     4441 gactaccgaa agattcagag caatcgaagg aatgaaagat gtttccaagg ataaaatcag
     4501 caagaagaga ctgataatcg gtgtctgggt tgctactttg accatactat ctcacctccc
     4561 cttgggtatt gctactctga gatgtaagtg gtttggtaat ggtcacttct ttcacatgcc
     4621 gaagaccctg actagtcatc tcaatccaat aatcaaccac agaaatcaaa gggtctaaac
     4681 gatgactaat gagcagaaaa cttcttcctt gattcctctc ctccacaatc cacttgcaaa
     4741 aataatggca ggctctatca tccaaacctg caaaaggttc atctagcaag atcacggaag
     4801 ccttactggt caagatggtc aagagctgaa gaattttttg ctgaccacca cttaattgat
     4861 agggactctt atcgactgcc tgctccaaat caaaatatcg taaagcttga aaaatccgct
     4921 gatttctttc agaatcaggt ccatctaatt gaagctcctc tcgcagactg actcggataa
     4981 actgcttctc agcttcctga acaatacccg tcagatcacg gtacaaactc tttttctttt
     5041 tcaggactgt ccccttccat gcaatgcgcc ccttatactt ttgaaattga agaatagacc
     5101 gaaagagggt tgatttcccg acaccattgt cacccaggat acaggaaatc ccttgataga
     5161 atgtgaaatc agcaattgaa aagagggggc gattaccaag ctcaccagtc acacggttca
     5221 tatggaataa ttccgggcta gaagcaactt cctttgaagc aacctgtgtc atctcatagg
     5281 aagggatttg aaacacttcc cttagttttc cgtctcttgc tcaaccatat ggtcgatata
     5341 ggctttatag tcagataaat catggtcgca caaaataact gtcttcccat caagagccaa
     5401 ctcttttaga atctccaata tctcgattct gctcttgcgg tcaatggaag cgaagggctc
     5461 atccaagaga tagaccctag gattcatggc aaagaggaca gccagcgctg ctttttgctt
     5521 ttccccacct gataggtgat gaatgggacg gtgtaagatt gcctcgcaac gacattgctg
     5581 gacaacctct gctattttag aatcaatttc ctgaaggtga tagccgatat tctccaaggt
     5641 gaaaatcagc tcctcaaaca agttctccat ggtaaattga tgattgggat tttgaaagag
     5701 aatgccaacc ttctggacac gttcgacgat agaaagctga ctgacctcac tcccatctat
     5761 caggacttga ccgctatagg gaagagaact gacttgggca atcatttgaa agaggctgga
     5821 ttttcctgaa ccactgctcc caactaacaa ggtaaaggct tgcgcatgaa aagtaaaatc
     5881 aaacggctca gagaaaattg gggactgaat cgctcgtagt tccagaccca tctatgcttt
     5941 tcctccagtt gcaaactgat gatagagttt gacaatggca cgaaccaaga tggtacagaa
     6001 gaaataaaca gaaataaaac gtaccacaag caaggaaagg acaaacggaa gggaaaaggc
     6061 gtagtaacct aacttaatgt attcatagac aaagctaaca agcgtaatcc caatactatt
     6121 agcagttaga gagagccaac tttcatagcg attcttagtt acgataaaac caaattcact
     6181 tcccaaacct tgaaccaagc cagacaaaag agctcctaga ccaaattggc taccataaag
     6241 gacttcagca agcgcagcta gcacttctcc aatcgttgca cttccgactc tcggaacaaa
     6301 gatggcagca atgggcgcag ccatacacca gagaccgaag aggatttcat tggcaaaggc
     6361 ctgcaaacca agaggtgtta agagtagact gagaatatta tacacatatc ctgaaccaac
     6421 gaaaacccca ccaaaaaaga tagacaagaa agcaagcaag ataacatctt ttaactgcca
     6481 ttttttcaac ataaaaaaac tccttttttt aaagaaaagt ggggcactca agaagaccga
     6541 cctaaatact ttgtata
//
LOCUS       DAVLWA010000095         6539 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLWA010000095 DAVLWA010000000
VERSION     DAVLWA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.095"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..423
                     /locus_tag="VYN80_001791"
     CDS             <1..423
                     /locus_tag="VYN80_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV1541659.1"
                     /translation="YNIITVKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEIF
                     VWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLAR
                     YLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYGS"
     gene            413..733
                     /locus_tag="VYN80_001792"
     CDS             413..733
                     /locus_tag="VYN80_001792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV1541660.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            796..2571
                     /locus_tag="VYN80_001793"
     CDS             796..2571
                     /locus_tag="VYN80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV1541661.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSVMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQALGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDDKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSENERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIIEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIQLILETGGL"
     gene            2572..3957
                     /locus_tag="VYN80_001794"
     CDS             2572..3957
                     /locus_tag="VYN80_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV1541662.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVKFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3980..4591
                     /locus_tag="VYN80_001795"
     CDS             3980..4591
                     /locus_tag="VYN80_001795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV1541663.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKAFAVAKSLKNSLMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            4667..4870
                     /locus_tag="VYN80_001796"
     CDS             4667..4870
                     /locus_tag="VYN80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541664.1"
                     /translation="MTVSGYLFQNFGLLDNENVSTDLDLGLISRKCNFKEKDKLKKDV
                     LARVGLTHIHLEQKIYELSGGEA"
     gene            4967..5077
                     /locus_tag="VYN80_001797"
     CDS             4967..5077
                     /locus_tag="VYN80_001797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541665.1"
                     /translation="MKLLLDMKNENRLIIIATHNPDIWNQADEVINLNQL"
     gene            5672..5959
                     /locus_tag="VYN80_001798"
     CDS             5672..5959
                     /locus_tag="VYN80_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541666.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            5961..6104
                     /locus_tag="VYN80_001799"
     CDS             5961..6104
                     /locus_tag="VYN80_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541667.1"
                     /translation="MSSEQQERMAVQYAEPSLLFTVRSLLKMLEWSRRQALEQDSAYK
                     IGV"
     gene            6117..6413
                     /locus_tag="VYN80_001800"
     CDS             6117..6413
                     /locus_tag="VYN80_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541668.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEVKSQITFK"
BASE COUNT         2188 a          961 c         1346 g         2044 t
ORIGIN      
        1 tataatatta ttactgtaaa acgtggttta tctcaaaata agagtcatgg ggatatttta
       61 caattacttt cagatgaagg aagtatttct gctaaagaat ttatatacat tgtagaaaat
      121 caagaaatat ttgtgtggtt caataaaata aatccaagct tagattcaat cttttcaact
      181 tatgaattga agatgcagga cgcaacaatt tcatcttctg agttagaatt tttatgtgat
      241 ttactattgt ataaaacttt agatcaagga aggtacaatg tagaggggcc gttagttctt
      301 gctagatatt tattgggatg tgagtttgaa gtaaagaatc tcagaatgat catatcagct
      361 cttcaaaata caattccctt tgaatcaata aaagaaagga tacgcccaca ttatggaagc
      421 taataagtat aaaattggca taattggtag ccgtgatatt attttaccat ttagcatgat
      481 tgggtttgat atatttcctg cctaccaaga acaagaagct ataaatacac taagaaaatt
      541 agctcaatct gattatggtg tcatttatat cactgaagac attgcttcaa tgatattaga
      601 tacaattcgc cattatgatt cccaagttgt gcctgctatt attttattac cgactcataa
      661 acaaggttta aatttaggat taaaacgtat agaggataat gtagagaaag cagtaggaca
      721 caatatttta taataatgta caaaattgtc tgtaatatta ttctataatt tttggactta
      781 gtaaggagaa taactttgac tcaagggaag attataaaag tatcgggacc tctagttatt
      841 gcatcaggta tgcaggaggc taatattcaa gatatttgcc gtgtaggtaa gctagggtta
      901 atcggtgaaa ttattgaaat gagaagagat caggcatcta tccaagtcta tgaagaaaca
      961 tctggtcttg gtccgggaga acctgttgtt acaactggag aacctctctc ggttgaatta
     1021 gggcctggat tgatttctca aatgtttgat ggcatacaac gcccattaga tcgatttaaa
     1081 ttggctactc ataatgattt tctagttcgt ggggtagaag ttccaagttt ggatagagat
     1141 attaagtggc attttgattc cactatatca attggtcaaa aagtgagtac gggtgatatt
     1201 cttggaactg tcaaggaaac cgaagtagtt aatcataaaa ttatggttcc ttatggaata
     1261 tctggcgaag tcgtttctat tgcctctggc gattttacaa ttgatgaagt tgtatatgaa
     1321 ataaaaaaat tggacggtag tttctataaa ggaacactta tgcaaaaatg gcctgtccgc
     1381 aaggcgcgtc ctgtttctaa acgtttaatc ccagaagaac cattaatcac aggtcaacga
     1441 gttattgata cattctttcc agtaaccaaa gggggagcag cagctgttcc tggaccgttt
     1501 ggagcaggaa agacagttgt acaacaccaa gtagctaaat ttgccaatgt tgatattgtt
     1561 atttatgtcg gttgtggaga acgtggaaat gaaatgacgg atgtactgaa tgagtttcct
     1621 gagttgattg accctaatac aggacaatca gttatgcaac ggacagttct gattgctaat
     1681 acttcaaata tgcctgttgc tgctcgtgag gcttcaattt atacaggaat taccatggct
     1741 gagtattttc gtgatatggg ctactctgtc gccattatgg ctgattcgac ttcacgttgg
     1801 gcagaagcgc tacgtgaaat gtcaggacgt ctagaagaaa tgcctggtga tgagggttat
     1861 cctgcttatc ttggaagtcg tatcgctgaa tattatgaaa gagcgggacg ttctcaggct
     1921 ctagggcttc cagaacgtga aggaacgatt actgctattg gagctgtatc gccacccggt
     1981 ggagatattt cagaaccagt tactcaaaac actttacgga ttgtgaaagt tttttggggg
     2041 cttgatgctc cgttggcaca gcgacgtcat tttcctgcaa ttaactggct tacctcttat
     2101 tcactctata aagacagtgt gggaacttat atagatgata aagagaagac agattggaat
     2161 agtaaaataa ctcgtgcgat gaactactta caacgggaat ctagtttaga ggaaattgtt
     2221 cgtcttgttg gaattgattc tctgtctgaa aatgaacgac taacgatgga aattgctaaa
     2281 caaattagag aagattattt gcaacagaac gcttttgatt cggtagatac attcacttcg
     2341 tttgcaaaac aagaagcaat gttaagtaat atcctaactt ttgctgatca ggcaaatcat
     2401 gctttagagt tgggttctta ctttacagag attatagaag gtaccgtggc agttcgagac
     2461 cgtatggcga gaagtaaata tgtttcagaa gatagattag atgaaatcaa aattatttca
     2521 aatgagatta cacatcaaat tcaattgata ttagaaacag gaggtctata aatgagtgtt
     2581 ataaaagaat atagaactgc tagtgaagtt gttgggcctc ttatgattgt tgaacaagtt
     2641 aataatgtgt cttacaatga gttagttgaa attcaacttc ataatggaga aattcgtcgt
     2701 ggacaagttt tagagatcca cgaagataaa gcaatggttc agctttttga aggatctagt
     2761 ggaataaatt tagaaaagtc taaaattcgt tttgctggtc atgcactaga attggctgta
     2821 tctgaggata tggttggtag aatttttaat gggatgggaa aaccaattga tggtggacca
     2881 gatttaattc cagagaaata tttagatatt gatggtcaag ctattaatcc tgtatctaga
     2941 gattatccag atgaatttat tcagacaggg atctcctcta ttgatcattt gaatactctt
     3001 gtacgtggtc aaaaattacc agtattttca ggttcgggct tacctcataa tgaattagca
     3061 gctcagatag caagacaagc gactgtttta aattctgatg aaaattttgc ggttgtattt
     3121 gcagcaatgg gtattacttt tgaagaagct gagttcttta tggaagaact cagaaaaaca
     3181 ggagcgatcg atcgttcagt cttatttatg aacttggcaa atgatcctgc aattgagcgt
     3241 attgcaactc cccgcattgc tttaactgcg gcagagtatc tagcttttga aaaagatatg
     3301 catgttctag ttatcatgac ggatatgact aactattgtg aagcgttacg tgaagtctcg
     3361 gcagctcgcc gtgaagttcc aggtagacga ggctatccgg gatatttata tacaaattta
     3421 tcaactctat acgaaagggc tggtcgctta gttggtaaaa aaggttcagt gacacagatt
     3481 cctattttaa caatgccaga agatgacata acacatccaa ttcctgattt aactggatac
     3541 attactgaag ggcaaattat tttgtcgcat gagttgtata atcaaggtta tcgtccacca
     3601 atcaatgttt taccttctct ctctcgatta aaagataagg gatctggaga aggtaaaact
     3661 cgtggagatc atgctccaac tatgaatcaa ctgtttgcag cctatgccca agggaaaaag
     3721 gttgaagagt tagcagtagt attaggagaa tcggctttat ctgatgtaga taaattgtat
     3781 gtgaagttta caaagcgttt tgaagaagag tacatcaacc aaggatttta taaaaatcga
     3841 aatattgaag atacgttgaa tcttgggtgg gaattactat caattcttcc tagaacagag
     3901 ttaaaacgta tcaaagatga tttgcttgat aaatacttac ctttggtaga agtttaatcc
     3961 agaaatggag tgattatcta tggtgcgttt gaatgtaaaa ccaactcgta tggaattgaa
     4021 taacttaaag gaacgtttga aaacagctga acgtggacat aagttattaa aggataaaag
     4081 agatgaattg atgaggcgat ttatttcttt gattcgtgag aataatcaac ttcggaaaga
     4141 agtagaaagt tatctaattg ataatctaaa agcctttgca gttgctaaat cattaaagaa
     4201 ctctctaatg gtggaggaat tattttcaat tccatcgaaa gaaattgaat tatttgttga
     4261 gaaagaaaat atcatgagtg taacagttcc tagaatgcat atgaatatta cttctcaaaa
     4321 tgagaacagt gaatacagct atttatcttc taatagtgaa atggatgatg tatttgctac
     4381 aatgaatagt ttaattgata aattactaag actggcagaa gttgaaaaaa cgtgtcagtt
     4441 aatggctgat gaaatagaaa aaacacgtag acgtgtaaat ggattagaat attcgattat
     4501 tccaaacttg tcagaaacta ttcattatat agaattgaaa ctagaggagg cagaaagagc
     4561 caatttagtt cggattatga aagtgaagta gatcctttat ttagatttta attagatgaa
     4621 ccaatatcag cttggataag gcggtaagcc tttctaagct tttttattga cagtatcagg
     4681 atatcttttt caaaattttg gtttgttaga taatgaaaat gtttctaccg atttagattt
     4741 aggattaata agtcgtaaat gtaattttaa agaaaaagat aagttaaaaa aagatgtatt
     4801 agcaagagtt ggtctaactc atattcatct agaacaaaag atatatgaat tgtctggcgg
     4861 tgaagcataa cgtgttgctt tagcgaaaat cattcttaaa aatccaccat taatactcgc
     4921 ggatgagctg actgcatcac tcgatccgac gacttcacaa ggaattatga aattattatt
     4981 agatatgaaa aatgaaaatc gtctaatcat cattgcaact cataatcccg atatttggaa
     5041 tcaagcagat gaagttataa atttaaatca attatagtta cagccaatat atattttcta
     5101 tattttaaag ttattttatc tgaatccgta aaagcagttc aatcttgtac tgcttttttc
     5161 ttttacgact tacgctgttt ggcttcatga gcaagccctc aaagtgaact aataacaaat
     5221 catgagaagt caaagtgatg aaatttctgt aacagaagta gagcgaattg ctgaaccatt
     5281 aaatgagtat atctcgaagt agaagcgtct attttgactt ttagtctgat agaatggtag
     5341 tttgaaaatg taaatgatag ctactgttca taaaacataa aaggtaagta cggtgttgtg
     5401 agaagagcta gaaattagca cttacctgat tattccaata acctcaaaga tgtgataaaa
     5461 atttcaacta ctcttatcta taataaagct acctgttctt tgaaaattga attcactccg
     5521 tcttgattag cgtgcctgtg taagtaggga ctgagagtat ttccctgtga agggcaactg
     5581 gcacaagacg tgtgtgagaa ttttctaaca gacacggagt ttatcgtttc cagactcaaa
     5641 cgatgcgaca aactagataa aggagttaat catgaaggta gtaaacttgt atgatttgaa
     5701 acaaatggga aataaaggtg gttgtaccat ccaattgatt catcactttc cctttggtat
     5761 gggcttagga catctcaaaa aagactacat tgaatttaaa cgtgttggta tctttgatgg
     5821 gaaagcagta gaagttactc ttcgagaacc ttattcgaga gatctactgc aggttgtcaa
     5881 gtcgattaag caacgtcaga aattgatagc ttatcgttat aaagaaggaa agctgctatt
     5941 tgttaagaag gaggtctgag atgtcctctg aacaacagga acgaatggca gttcaatatg
     6001 cagagcctag tcttttattt actgtaagaa gtctcttaaa gatgctagaa tggtctagac
     6061 gtcaggcttt agaacaggat tccgcctata agataggggt gtagaaatta gaagaattgc
     6121 tacaatctcc ctatgcgatt gatacgatta atctgaaaaa agatttttta gaaaaaccaa
     6181 ttgatataga gaaatttaaa gcttttttag aaaaagaaga gattccttta gccatcgctt
     6241 ggcaagggga ttccctgcat ttctacacga aagaccgttc gattctagac aatcatttag
     6301 accatctgtt agaaaaaatg gtgaatgatc ctgagaaatt atctgatttt tccatggata
     6361 agtcattaga cgatgcaatt gatgaggtta aatcccaaat tacctttaaa taatagagta
     6421 tcgcaatttg attgcggaac tcttgtttaa tgagagttga gataatataa aggcgataat
     6481 aattgggaga ataattatat tccccgccaa accccactaa gtttttatct tggtggggt
//
LOCUS       DAVLWA010000096         6156 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLWA010000096 DAVLWA010000000
VERSION     DAVLWA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.096"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            65..769
                     /locus_tag="VYN80_001801"
     CDS             65..769
                     /locus_tag="VYN80_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1541669.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    828..915
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            951..1265
                     /gene="rplU"
                     /locus_tag="VYN80_001802"
     CDS             951..1265
                     /gene="rplU"
                     /locus_tag="VYN80_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1541670.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1281..1625
                     /locus_tag="VYN80_001803"
     CDS             1281..1625
                     /locus_tag="VYN80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1541671.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1642..1935
                     /gene="rpmA"
                     /locus_tag="VYN80_001804"
     CDS             1642..1935
                     /gene="rpmA"
                     /locus_tag="VYN80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1541672.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2246..2451)
                     /locus_tag="VYN80_001805"
                     /pseudo
     CDS             complement(2246..2451)
                     /locus_tag="VYN80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2888..3805
                     /locus_tag="VYN80_001806"
     CDS             2888..3805
                     /locus_tag="VYN80_001806"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1541673.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3898..4746)
                     /locus_tag="VYN80_001807"
     CDS             complement(3898..4746)
                     /locus_tag="VYN80_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1541674.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            4887..5726
                     /locus_tag="VYN80_001808"
     CDS             4887..5726
                     /locus_tag="VYN80_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1541675.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5833..6108
                     /locus_tag="VYN80_001809"
     CDS             5833..6108
                     /locus_tag="VYN80_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1541676.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT         1970 a         1034 c         1266 g         1886 t
ORIGIN      
        1 atctaaatgg tagcctacga ttttttagaa agaaaatggt acaatatttc taagagaaaa
       61 tacaatggga ggtaaaatga ggttattacc tataagaaaa atatcacgtc agtctaaaag
      121 gttagcactt tttttgacgt tttgtgctgg atatgtggat gcttacactt ttattgttcg
      181 cgggaatacc cttgtagctg gacaaactgg aaatgttgtc tttctttcag tagaattaat
      241 taaaaataat gtttcggatg ttagggacaa ggttctcacc ttgctagcgt ttatgatggg
      301 agtcttttta ttaacgattt ataaggaaaa attgagaatt gtgaaaaaac ctattctgtc
      361 cttgattccc ttggcaatct tatcaatcat tattgctttt gtgccgcaaa ctgtggataa
      421 tatctatcta gtgccgccct tggccttctg tatgggactg gtgacaactg cttttggaga
      481 agtgtcgggt attgcctata ataacgcttt tatgacaggg aatatcaaac ggaccatgct
      541 ggcttttgga gattatttcc gaaccaagca cactcctttt ttgcgtgaag gattcatatt
      601 tgttagcctg cttagtagtt ttgtccttgg cgttgtcttt tcagcctatt tgacgatttt
      661 ctatcatgaa aagaccattc ttggtgttcc tattatgatg agcgtttttt acctcagcat
      721 gctttttgcc tcttggcaga aaaaagtaaa agaaaaagct tcattttagg tgttattact
      781 tgtaagaaaa taggagatat gctatacttg aagagttgac tatgcacgat cctgtgcaac
      841 cgcacgaaca tcgttgcttg attattcaag tttaagtggt tcgtcccacg atgctaggcg
      901 agtcttcaca aaaaatcggg gaaacccata aaaatcatag gaggtgcata atgagcacat
      961 acgcaattat caaaactggc ggaaaacaag ttaaagttga agttggtcaa gcagtttacg
     1021 ttgaaaaatt gaacgttgaa gctggtcaag aagttacttt taacgaagtt gttcttgttg
     1081 gtggtgaaaa cactgttgtc ggaactccac ttgttgctgg agctactgta gttggaactg
     1141 ttgaaaaaca aggaaaacaa aagaaagtgg ttacttacaa gtacaaacct aaaaaaggta
     1201 gccaccgtaa acaaggtcac cgtcaaccat atacaaaagt tgtcatcaac gcaatcaacg
     1261 cttaatttta aggagaacac atgatacagg cagtctttga gagagccgaa gatggcgagc
     1321 tgaggagtgc ggaaattact ggacacgccg agagtggcga atacggctta gatgtcgtgt
     1381 gtgcatcggt ttctacgctt gccattaact ttatcaattc tattgagaaa tttgcaggct
     1441 atgaaccaat cctagaatta aacgaagatg aaggtggcta tctgatggtt gaaataccaa
     1501 aagatcttcc ttcacaccag agagaaatga cccagttatt ctttgaatca tttttcttag
     1561 gtatggcaaa cttatcggag aactattctg agttcgtcca aaccagagtt atcacagaaa
     1621 actaacacgg aggaaaacat tatgttaaaa atgactctta acaacttgca acttttcgcc
     1681 cacaaaaaag gtggaggttc tacatcaaac ggacgtgatt cacaagcaaa acgtcttgga
     1741 gctaaagcag ctgacggaca aactgtaaca ggtggatcaa tcctttaccg tcaacgtggt
     1801 acacacatct atccaggtgt aaacgttggt cgtggtggag acgatacttt gttcgctaaa
     1861 gttgaaggcg tagtacgctt tgaacgtaaa ggacgcgata aaaaacaagt ttctgtttac
     1921 ccaatcgcaa aataaaaagg ttcattgaac cttttatccc gaaccttgaa atgtaaaggt
     1981 gaggaagcta gaaacagctt aaaataaggc tttccgagtt ttcggagagc ctgttttttt
     2041 attttgatac ccatactttt taatatgtat agtggattga tactcttcga aaatcaaatt
     2101 caaaccacgt caatttcacc ttgccgtact caagtacagc ctgcggctaa cttcctagtt
     2161 tgcactttaa ttttcattga gtatgaatct ggaatagaac actgttaaaa attgatatga
     2221 gtttcctact cgatttcttc agattttatt tcaattgact ataagaactg ctatctggtt
     2281 tcaaatattg gcttgtcgtc ccttcacttg tctgaatggt ttgagccgtt ccttctgata
     2341 agggaaaaga tattctttag ctgaaacggt ctgagaatcc aagctaacag atggagagaa
     2401 aaatagccca gtctgaagca cactagatgg gagttgttgg aaagcagtca tctagttttt
     2461 ctttgctgtt ttggcttttt tctttgtcat cagggaaagt gacttgcttc aaatcaggga
     2521 gtttaggaaa ttcttcaaac atcttgccca agttgtctca ttttgtaaat tttacagcca
     2581 taggtgtacc tcgattctaa attctacctt tttggcacca attagtaatt taaagttctc
     2641 aggagttagt tgcattgctt tttcatacat aacatagtat gacactttat ggttaccgaa
     2701 caagtctaat cactccttgc aaaattttga agatgtccta caagtttttg agaagaatat
     2761 tgcaaagttc atagttaagc aacgatagaa taaagtgctt tttgaaaaca ggtacacttc
     2821 atgtgaaaaa aacaacattt cagattttct gttccagaaa tagctatcgc tatgatataa
     2881 ttattttatg attattacta ttcctataaa aaaccaaaaa gatattggca caccatctga
     2941 ttcagtcgtt gttctcggct attttgatgg catacataag gggcatcaag aattatttcg
     3001 tgttgccaac aaggctgcga gaaaggattt attgcctatc gtcgttatga cctttaatga
     3061 atctccaaag atcgctttag agccttatca tccagatttg tttttgcata ttttgaaccc
     3121 tgctgaacgt gaaagaaaat taaagcgcga aggtgtagaa gaattatatc tccttgattt
     3181 tagtagtcaa ttcgctagtc tcactgcaca agaattcttt gcaacttata tcaaggctat
     3241 gaatgccaaa attattgttg caggttttga ttatacattt ggttctgaca aaaaaacagc
     3301 agaagattta aaggattact ttgatggaga agttatcatt gttccacctg tagaagatga
     3361 gaaaggaaag attagttcaa ctcgtatccg tcaagctatt ttagatggaa atgtgaaaga
     3421 agcaggaaaa cttttggggg caccgcttcc atcaagaggt atggtggttc atggtaatgc
     3481 tcgtggtcgt acaattggtt atccgacagc gaatttagtg cttttagacc gtacttatat
     3541 gccagcagat ggcgtttatg tcgttgatgt tgagattcaa agacagaagt atcgtgctat
     3601 ggctagtgtc gggaaaaatg tgacctttga tggagaagaa gcacgttttg aagtcaatat
     3661 ttttgatttt aatcaagata tttatgggga aaccgtcatg gtttattggc ttgatcgcat
     3721 tcgtgatatg accaaatttg actcagttga ccaattagtg gatcagttaa aggctgatga
     3781 agaagtaact cggaattggt cttaagagct tgagtaaata aaacaaaaaa gaggttgtct
     3841 gtaacccaaa agatagatga tttagtctaa cttttgaggt cacgacatta cctcttttta
     3901 ttctttttca aaggtgaagc cttctcctag gatttcatgg gcttctgtaa tagttataaa
     3961 ggcttgagga tcgattcgat gaatcatttc cttcgttttc acaatttcat ttcttccgac
     4021 aatacagtag atgattttca aattttcttt actatagtag ccttgaccag aaataaaagt
     4081 aacacctctt ccgaggtcat cattaatcgc cttagcaagt tggtcaggac gttttgtgat
     4141 aatcataaag cctttgccgg catatcctcc ttcaccaatc aaatcaataa cacgagaaac
     4201 aataaaatca aacaaaagcg tgtaggaaac caatctcaaa tccttgaaga ttaggagaat
     4261 caacatgaga atacaaaaat ctaagataaa gagcagtttt cctatggata tatgagtgta
     4321 tttgttgaga atacgagcta gaatatcagt tccgccagtt gtacctccag cattaaaaat
     4381 aattccaagg ccaattccca ataggattcc cgctataagg gctgtgatta gtaaatcacc
     4441 ttgaagatca atatgaaggg gaatatgctc aaaaaaagct aaccaggcgg acaaagctaa
     4501 ggttcctagt aaactagaat agagggattt ggctccaaag atcttccaag ctaggatgaa
     4561 aaggggaata ttaatcagca ggttcatgag ggaaacaggg attttaaaaa gataaaaggt
     4621 gatgagggta atgcctgtcg cccctccttc aaagagatga tggggaacta caaaataagt
     4681 cagtccaaaa gcataaatag cagcacctag taaaatggta aaaatggggt aaattttttt
     4741 aatcatagga cacctctttt cttataagtt gattatatca aattttacgg aaaatttgat
     4801 tgactccatt aggattttta atcttttttt gatataatta gaagtaagga aaccaatctg
     4861 aatcctaaaa tagaaagatt gatactatga caaaaattaa gattgtaacc gattcatctg
     4921 ttactattga acctgaacta gtaaagcaat tagatattac aattgttcca ttatctgtaa
     4981 tgattgataa tgttgtttat tctgatgcgg atttgaaaga agaaggtaaa tttcttcagt
     5041 tgatgcaaga aagtaagaat cttccgaaaa caagtcagcc acctgtaggt gtctttgctg
     5101 agatttttga agacctatgc aaagatggtg gccagattct tgctattcat atgtcccatg
     5161 ctctttcggg tacggtagaa gcagcacgcc aaggtgctag cctatctact gcagatgtga
     5221 ctgttgttga tagttccttc actgaccaag ccctgaaatt ccaagttgtt gaggctgcga
     5281 agttagctca agaaggtaaa gatatggagg caattttatc tcatgtagaa gaggttaaaa
     5341 accatacaga gctttatatt ggtgtttcaa ctttggaaaa tcttgtcaaa ggtggacgaa
     5401 ttagccgtgt aactggcttg ttgagctctc ttctcaatat ccgtgttgtc atgcaaatga
     5461 aagaccatga attgcagcca atggttaaag gtcgtggaac taaaacattt aaaaaatggt
     5521 tagatgagtt gataacatcg ctttctgaac gtgctgtagc agagattgga atttcatatt
     5581 ctggtagtga tgattgggca aaagagatga aagaaagctt acaagcttat gttgaaaagc
     5641 caatttctgt tttggaaacg ggttccatta ttcaaactca cacgggtgag aacgcttggg
     5701 ctattttgat acgttaccac tcctaaaaaa ataaataaaa tgggaagaaa tagcgttttt
     5761 aacttgacct aaaagggatt ttaggatatg attatatttg ttaattagaa attatttgga
     5821 ggaatcatta acatggcaaa caaacaagat ttgatcgcta aagtagcaga agctacagaa
     5881 ttgactaaga aagactcagc agcagcagtt gaagctgtat ttgcagcagt agctgactat
     5941 cttgcagctg gtgaaaaagt tcagttgatc ggttttggta actttgaagt tcgtgagcgc
     6001 gcagaacgta aaggtcgcaa cccacaaact ggtaaagaaa tgacaattgc agcttctaaa
     6061 gtaccagcat tcaaagctgg taaagctctt aaagacgctg ttaaataatc agtctttaaa
     6121 aagcctattg tatcaagctt tctagcttgg tcagta
//
LOCUS       DAVLWA010000097         6117 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLWA010000097 DAVLWA010000000
VERSION     DAVLWA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.097"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VYN80_001810"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VYN80_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1541677.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1320..2024
                     /locus_tag="VYN80_001811"
     CDS             1320..2024
                     /locus_tag="VYN80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1541678.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2017..3366
                     /gene="vicK"
                     /locus_tag="VYN80_001812"
     CDS             2017..3366
                     /gene="vicK"
                     /locus_tag="VYN80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1541679.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3368..4177
                     /locus_tag="VYN80_001813"
     CDS             3368..4177
                     /locus_tag="VYN80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1541680.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4268..4510
                     /locus_tag="VYN80_001814"
     CDS             4268..4510
                     /locus_tag="VYN80_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1541681.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4512..4766
                     /locus_tag="VYN80_001815"
     CDS             4512..4766
                     /locus_tag="VYN80_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1541682.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4841..>6117
                     /locus_tag="VYN80_001816"
     CDS             4841..>6117
                     /locus_tag="VYN80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV1541683.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYR"
BASE COUNT         1941 a         1016 c         1380 g         1780 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat tcctggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagcattga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggtccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttt ggtataatag tagagaaaaa ggtgaacata
     1321 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
     1381 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg ctagagcaat
     1441 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
     1501 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
     1561 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
     1621 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
     1681 ctatgccagt agatggtcag gaagcagata gtaaacctca acctatccaa attggggatt
     1741 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
     1801 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
     1861 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
     1921 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
     1981 tgacgcgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
     2041 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
     2101 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
     2161 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
     2221 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
     2281 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
     2341 gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
     2401 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
     2461 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatatc
     2521 aatggtgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
     2581 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
     2641 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
     2701 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
     2761 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
     2821 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
     2881 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
     2941 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
     3001 acgcaggttg tcgacaatat tttaaataat gctattaagt attcgccaga tgggggtaaa
     3061 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
     3121 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
     3181 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatt
     3241 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
     3301 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
     3361 gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt ccagtggaaa
     3421 ttctttttat ctggaaacct caaaaaagaa gcttttagtg gatgcaggct tgtctggcaa
     3481 gaaaattacc agtctgctag ctgaaattaa ccgcaagcca gaagacctgg atgccatctt
     3541 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
     3601 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
     3661 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cttttggaga
     3721 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
     3781 tatgaaggat gacaagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
     3841 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
     3901 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttggtca
     3961 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gcactaagaa
     4021 gatttacctt gggcatttat ctaaggaaaa caatatcaag gaactggctc atatgaccat
     4081 ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg tttatgatac
     4141 ctcaccagat accgcaacac cattgacaga gatatagaaa gaacgctgag aaggtgttct
     4201 ttttatattg actgaacacc taaaaagtaa tacaatggtg ttaccattaa aaaagggagc
     4261 acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat ttatgaaagc
     4321 aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa ctcttgaagc
     4381 cctagaagat gaatacgatg ctcgtgtggc agatttagcc tatcaagagt atttagaaga
     4441 cttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt taggcttgaa
     4501 ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa ttgaaaaaat
     4561 tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt ttggaagacc
     4621 caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc tatagaattg
     4681 gtaattatcg agttatcgta caaattgtag atgatgaatt agtcgttgct actctagaag
     4741 ttggtcatcg gagagatatt tattgaatta cttttttcaa gtaaattttc attctttaaa
     4801 atatgatata atagggctta caatataaga gaggttgctg atgacaatag atattagtga
     4861 agaaagtttg gctaaagaat cggctgactt attaaaaatt cttttaaaag atcgaacgac
     4921 aaagaaatca attgtttggg ctacgcattc ttatgaattg ttaggaaagg gatttgctcc
     4981 aagtgatcgc atcaatccaa gtaaggttac tggaaacttt gccaacttga ttcagcccag
     5041 atctgaaaaa tccaaatatg aacaaaaaga tcggacaaaa ataagggcag aagtgtttac
     5101 accaacgtgg ttggtcgcaa agcaaaatgg atatgtagaa tcgaaattag gctcgttaag
     5161 tcttgaggag tatgtggact tacgatggtt agaggtaact tgtggtgaag caccttatat
     5221 ggtaacgaga tatgataccg taactggtga ggaaattcca ttatctgagc gagttggttt
     5281 tgttgataga aaactgcaac ggattagtcg tgaggtttca gatgaagtca cattttataa
     5341 acttgtaaaa gatgcttatc atgcttcgta tggttatgag tatcaaggag attctctcct
     5401 tttggcgcgt gagaatcttt tggcaacttt tgaggactat tatctagcta agactggaaa
     5461 tcaaccaaca ttagagcaaa agaaagaaat tgcgactatt atctcttaca atgtctttca
     5521 gatggatgga ttgaaaaaaa gtagtcctta ttcagctaaa caaaagcaat ctcaacaatt
     5581 gagtttgttt gcggatgagc tagaagtcca agaagtagaa gaatctaaaa cccatatcaa
     5641 agactggaaa aagaatagaa tgataggttt tgagcgccta tctagcgagg aaagtgaaat
     5701 gaaatttgat gtcgtgatag gaaatcctcc gtatcaggaa acaggagaag ccagagatga
     5761 gccgatatat cactatttta ttgatgaagc ttataaaatc gcagataaat ctatactaat
     5821 caccccagca aggtttttat ttaaagctgg tcaaactcct aaaaattgga tggaaaagat
     5881 gcttgaagac aagcatttaa aagttcaatt ctatgaactt aaaagcggta aagtatttac
     5941 aggaacggat attaagggtg gagtagcaat aacttatcgt gatgttgata aagatttggg
     6001 accaatcgga gtatttacta tttttgaaac tttgaatggt attgtaaaaa aaatcgaaaa
     6061 acatccagaa tttgaaactt tttctgattt agtttatcct caaggtattt atagatt
//
LOCUS       DAVLWA010000098         6053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLWA010000098 DAVLWA010000000
VERSION     DAVLWA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.098"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..1151
                     /locus_tag="VYN80_001817"
                     /pseudo
     CDS             1..1151
                     /locus_tag="VYN80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1266..2069)
                     /locus_tag="VYN80_001818"
     CDS             complement(1266..2069)
                     /locus_tag="VYN80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1541684.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2350..3105)
                     /locus_tag="VYN80_001819"
     CDS             complement(2350..3105)
                     /locus_tag="VYN80_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1541685.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCFSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(3175..5826)
                     /locus_tag="VYN80_001820"
     CDS             complement(3175..5826)
                     /locus_tag="VYN80_001820"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1541686.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(5838..>6053)
                     /locus_tag="VYN80_001821"
     CDS             complement(5838..>6053)
                     /locus_tag="VYN80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1541687.1"
                     /translation="LNHNTLVDIKSKLESLKLKHSRYYISKISKYDLKYNPGNYTMSP
                     LAAFAEIEEIYIELSKIGLVKIVNKSN"
BASE COUNT         1785 a         1308 c         1126 g         1834 t
ORIGIN      
        1 aaaattcctt accttgaaac gactggtatg cctactagaa ttctccttag aaaacgtcgt
       61 tttaagtgct atcattgctt gaaaatgatg gtcgctgaaa cttctatcgt caagaaaaat
      121 caccaaattc ctcgtattat caaccaaaag attgcgcaaa agttaattga aaagatttct
      181 atgaccgata ttgcccatca gctggccatt tcaacttcaa ctgtcattcg aaagctcaat
      241 gacttccact ttgagtgtaa ttttagaaat ctgcctaaga ttatgtcttg ggacgttgaa
      301 acagtccggg gagtgactgt ttcaatcggg agatggagat gagctttatt gcgcaagatt
      361 ttgaaaagct cgatatcatc actgttcttg agggtagaac acaagctgtc atccgagatc
      421 actttcttaa atatgataga gccgtccgat gtcgcgtcaa aattattact atggatatgt
      481 ttagtcctta ctatgactta gctaaacagc ttcgctttca aatttctagg ctcaggctga
      541 aacagtctcc cagactgttt cactcccgaa tgctaaaatc gttcttgatc gctttcatat
      601 tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtc caaatcatga atcagtttca
      661 tcgaaaatcc catgaataca aggctatcaa gcgctactgg aaactcattc aacaggatag
      721 ccgtaaactc agcgataaac atttttatcg ccctactttt cgtatgcatt taaccaataa
      781 agagatttta gacaagcttt tgagctattc acaagacttg aaacatcact atcagctcta
      841 tcaactcttg ctgtttcact ttcagaataa ggaaccggag aaatttttcg aacttatcga
      901 ggacaatctt aagcaggttc atcctatttt tcagactgtc tttaaaacct tcctcaaaga
      961 taaagaaaag gttatcaacg cccttcaact acactattct aatgccaaac tggaagcgac
     1021 caataatctc atcaaactta tcaagcgcaa tgcctttggt tttcgaaact ttgaaaactt
     1081 caaaaaaacg gatttttatc gctctgaaca tcaaaaaaga aaggacgaaa tttgtccttt
     1141 ctcgaactta gcttttcttc aacccactac aattgacaaa gagccaaact ttgatgatat
     1201 acgttttctt gtgagaatat ttacttcatt ttttcctaaa attcaatgtt tactcagtat
     1261 ttggattatg aaaaatcgag gtctaaatct agatacattt tttctgaaga caaatcattt
     1321 tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa actcaggacg tcgctgtaaa
     1381 atctttgcat tatctaatac caaggcatca cgaaaatact tggaatgttg ctgaaatggt
     1441 atattatcaa tatcaaaacc aaactcacga agatactgaa tcaaaaagac cgttactgtc
     1501 cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc ctgaaataaa atgctggatt
     1561 tgtttaacca catccgcctg agttagtgtc gcatatgcaa cttgtttttc ctgattaaaa
     1621 tcataatcta aggtcatttg aatcatggag taatcagagt acacaacact ttcaccattc
     1681 aaaacaggtt cattctttgt gatattggtc tgacgaaatt gccccaccgg aatagagggt
     1741 tcaaatatat cttgaaacaa ctccttatga atagcaagta aggtcgcagg actaaagcta
     1801 aagcctcttc gagacaatag ttctacaata cgtagagaaa ccaagtctgc ctcttccgta
     1861 cttgcatcaa tagtatggtg ataagccgtt gcatcctcat aaacctgctc ataagtcagt
     1921 tctccccggg actgtttctc agccaaagat tccatatacg ctgatggcac tagattgtca
     1981 actttctgca gaccaaaacc tatccgccat aaatcacgct tcgcttcata agacaagttt
     2041 ggattgtcaa tgttgtaagt tggttgcata gaaatatcct ttcaattgtt tttcttcatt
     2101 accagtaaat cacgaaattg gttggaaact ggcttaaact ataaaaagtc ctcacaatag
     2161 gttctatgat atatatatat atatacttca tgtaaataat aaaagagtta taatcagcac
     2221 tactgataaa ttcaataccg atttcgccat cttaaaactt agtcaaacga ataccgttga
     2281 tttccagttc attaagtcgt tgacctgtca agacttctcg ttccttcaac tggaaaagtt
     2341 aagggggaac taatacaaat cctctacgct tctatattca atgatttcat ttttgataat
     2401 attcttttta aactcaaaat gttttaaagg attatcgatt aacactaact ttggaatatc
     2461 atctagattt gtgaccaagg ataaaataaa atccgattga aactccttga ctttttcaaa
     2521 ttcttttgca gttaaacgca cacgcccctt agattttcgg atacctttta cttcggcaag
     2581 gtaagaatac tcttggacat ctacctgaaa gtcatatcca tcaccataaa gacgggcatc
     2641 ggttaactgt ccaccttgga atttttcttc tttatcaaaa tgaaggataa aataattctc
     2701 agcctccatt cccgtttcct gtagacgttt gaatttagtt cgaactattg gtttttcaac
     2761 gatggttatg cctgttttct tcccttcact ggcaagtaac attttaacta tttcagcata
     2821 actatgaaca tcttcatttc caaacatcat atcaattaaa tctttacgaa aacgataaac
     2881 ttcagctttc tgccaccaac cttttcgatt attgtcaaaa taaggatcaa ataaatccat
     2941 tctatttttt acaactccag ttgtttcagc aattcccaga gaaaccacat agcgataaaa
     3001 ctccgacttg ctaaagcatt gaaattcttt tataaaaagc ttgtcaaact tggcaagacc
     3061 gtagccaatt aaatttaaca attcataatg agggtgtttg gacatcactt tctccatcaa
     3121 tagatattga tttcattata ccaaaaaaca cggctcatca ccgtgtttct gtgtttattt
     3181 caccaacttc ttcatctcat caatacgtgc tacggtcaca tcatacttgg cttggtagtc
     3241 ggcttgtttg tctttttctt tttggacaac ttctggttta gcgttggcga cgaagcgttc
     3301 gttagagagt ttcttaccaa ccatgtccag ttctttttgc catttagcga gttctttgtc
     3361 gagacgagct agttcttctt caacattgag gagatcggcc agtggcaaga agatttcggc
     3421 tcctgtaata acgcttgaca tagccagttc aggtgcaggg atggttgatg cgatttccaa
     3481 gtgttctgga tttgtaaagc gtttgatata gttgacattg ctgttaaaga aggcttccaa
     3541 gtcactatcg cttgtcttaa caaggatggt gataggcttg cttggtgcta catttacttc
     3601 cgcacgcgca ttccgaacag cacgaattaa gtctttgaga ctttccacac cagtgtgagc
     3661 cgcaaggtct tcaaaggcta gattaacagt tgggtatgca gctgtcacga tagaaccttc
     3721 tgagatttgt ccaaagattt cctctgtcac gaatggcatg attgggtgaa ggagacgaag
     3781 gatcttgtcc aaagtgtata gtagaacaga acgtgtgatg actttctctt cttcgttatc
     3841 gctataaagg acttccttgg tcaactcaac ataccagttg gcaaattctt cccaaatgaa
     3901 gttgtaaagg atatgaccag ccacaccaaa ctcgaactta tcaaagtttt cagtaacttt
     3961 tgcaatggtt tcgttgagat tgtggagaat ccagcggtcc gtcacattac cagcctcacc
     4021 tgttgcaact tttgtgacat tgtcatgcgc cacatccagc gtcaaacctc cattgttcat
     4081 gaggatatag cgagaaatgt tccaaatctt gttaataaag ttccatgaag catccatttt
     4141 ctcataagag aaacgcacgt cttgtcctgg cgcagaaccg tttgaaagga accatcgaag
     4201 ggcatcggca ccgtatttct cgataacatc cattgggtca atcccgttac cgagagactt
     4261 agacatcttg cgtccttgct cgtcacgaat gagaccgtgg ataaggacgt tttggaatgg
     4321 ttgacggcct gtgaattcca atgactggaa gatcatacga gacacccaga agaagatgat
     4381 gtcgtaacct gttaccaagg ttgaagttgg gaaataacgt ttaaagtctt ctgagtcgac
     4441 ttcaggccag cccatggttg aaaatggcca gagggcagaa ctgaaccaag tatccaagac
     4501 gtcttcgtcc tgagtccatc cgtcaccttc tggagcttct tcgccgacat acatttcacc
     4561 atcagcattg taccaggcag ggatttggtg accccaccaa agctgacgag agataaccca
     4621 gtcgtggaca ttttccatcc attgaaggaa ggtatcgttg aaacgaggtg ggtagaattc
     4681 gaccttgtcc tctgtgtctt ggttggcaat ggcgttctta gccaattggt ccatcttgac
     4741 gaaccattga gtagacaagc gtggctcaac tacgacacct gtccgttctg agtgaccgac
     4801 tgaatggaca cgtttttcga ttttgacaag ggcaccgatt tcttccaact tagcaacgac
     4861 tgccttacga gcttcaaaac gatccatgcc tgaaaattca aaggcaagct cattcatagt
     4921 tccgtcgtcg ttcatgacgt tgacttgtgg caagttatga cgttggccaa ccaagaagtc
     4981 atttggatcg tgggcaggtg tgattttcac gacaccagta ccaaactcag gatctgcgtg
     5041 ctcatctcca acgattggga tgagtttatt agcgattgga aggatgacgt ttttaccaat
     5101 caagtccttg tagcgcgggt cttctggatt aaccgcaacc gcaacgtccc caaacatagt
     5161 ctcaggacga gttgtagcaa cttcaagggc gcgtgaacca tcttccagca tgtaattcat
     5221 gtggtagaag gcaccttcta catccttgtg aatcacctca atatcagaaa gggctgtgcg
     5281 agctgctggg tcccagttga tgataaactc accacgatag atccagcctt tcttgtaaag
     5341 gtccacaaag accttacgaa cagcttttga caaaccttcg tcaagagtga aacgctcacg
     5401 agaatagtct acagaaagcc ccatcttgcc ccattgttcc ttgatggtag tggcatattc
     5461 gtctttccat tcccagacct tcgtcaagaa agactcacga cctaggtcat aacgcgtaat
     5521 accctcacca cgtaagcgct cctcaacctt agcctgagtc gcaataccag cgtggtccat
     5581 acctggaagc caaagggtat caaagccctg catgcgtttt tgacggatga tgatatcctg
     5641 caaagtcgta tcccaagcgt gaccaaggtg aagtttccca gttacgtttg gtggtggaat
     5701 cacgattgaa taaggcttag ccttttgatc gcctgaaggc ttgaaaacat ccgcatcaag
     5761 ccatttttgg taacgaccag cctcaacctc ggctggattg tatttagatg aaagttcttt
     5821 agacatgtgt gttctcctta attactttta ttgacaattt taaccaaacc aatttttgaa
     5881 agttcaatat aaatctcttc aatttcagca aatgctgcaa gtggtgacat agtataattt
     5941 ccaggattat atttaagatc atacttgctt atctttgaaa tataatacct agagtgtttc
     6001 aattttaatg attccaactt tgatttaata tctactaatg tattatggtt taa
//
LOCUS       DAVLWA010000099         6007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLWA010000099 DAVLWA010000000
VERSION     DAVLWA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.099"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..324
                     /locus_tag="VYN80_001822"
     CDS             <1..324
                     /locus_tag="VYN80_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1541688.1"
                     /translation="VFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVR
                     AQSRVPFSEEKDIAFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKV
                     LNEEK"
     gene            326..2548
                     /locus_tag="VYN80_001823"
     CDS             326..2548
                     /locus_tag="VYN80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1541689.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            2588..3697
                     /locus_tag="VYN80_001824"
     CDS             2588..3697
                     /locus_tag="VYN80_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1541690.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3854..4744
                     /locus_tag="VYN80_001825"
     CDS             3854..4744
                     /locus_tag="VYN80_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1541691.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            4769..5092
                     /locus_tag="VYN80_001826"
     CDS             4769..5092
                     /locus_tag="VYN80_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541692.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            5082..>6007
                     /locus_tag="VYN80_001827"
     CDS             5082..>6007
                     /locus_tag="VYN80_001827"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1541693.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEG"
BASE COUNT         2125 a          852 c         1206 g         1824 t
ORIGIN      
        1 gtttttgcaa attgtatgac ttatctagct gatggagttc caggagatat ttttgaaact
       61 cataagaaat atttagatat tcatattgtt gttgaaaata ctgaaaaaat ggcagtaact
      121 tccccagttc gtgcccagtc acgtgtacct tttagcgaag agaaggatat tgctttttac
      181 gatagcaaag actatcaaat tgttgaattg cttccaggga atatgttggt gacttttgaa
      241 gaagatcttc accaaccaaa gattcattgc aatgatgaaa ctgttaaaaa acttgttatc
      301 aaagttttaa acgaggaaaa ataaaatgaa aaaaaatatt aaacaatatg taaccttagg
      361 tactgtagtg gtattatcag catttgttgc taactcagtt gcagctcagg agactgaaac
      421 ttctgaagta tcaacaccaa agttggtgca acctgttgca ccaacgactc cgatttcgga
      481 agtacaacct acatcggata actcttcgga agttactgta caacctcgaa cagttgaaac
      541 tactgttaag gatccatctt ctacagcgga agaaactcct gtcttagaaa aaaataatgt
      601 tactttaaca gggggcggag aaaatgttac taaagagtta aaggataaat ttactagcgg
      661 tgactttact gtagtgatta agtacaatca gtcaagtgag aaaggcttac aagctctgtt
      721 tggaatatct aattccaaac ccggtcaaca aaatagttat gtagatgtgt tccttagaga
      781 caatggtgag ttggggatgg aagcgcgtga tacttcttcc aataaaaata acctagtatc
      841 cagacctgct tcagtttggg gtaagtacaa acaagaggct gtgactaaca ctgttgcagt
      901 agtagcagat tcagtcaaaa aaacatattc tttatacgca aatggtacaa aagtagtaga
      961 aaagaaagtg gataatttcc taaacatcaa ggatattaaa ggtattgatt actatatgct
     1021 tgggggagtg aaacgtgcag gaaaaacggc gtttggtttt aacggaacac tagaaaatat
     1081 caaattcttt aatagtgcat tggatgaaga aactgttaaa aagatgacaa caaacgctgt
     1141 tactggacat ttaatttata cggctaatga tacaacaggt tctaactatt tccgtattcc
     1201 agttctgtat acttttagca atggtcgggt attttcaagc attgacgctc gttacggtgg
     1261 aactcatgat ttcttgaata aaattaatat tgctacaagt tatagtgatg ataatggtaa
     1321 gacatggact aaaccaaaat taacattggc attcgatgat tttgcgccag taccattaga
     1381 atggcctcgt gaagttggtg gacgtgactt acaaatcagc ggtggtgcaa cctatattga
     1441 ctctgttatt gttgaaaaaa agaacaaaca agtactcatg tttgctgatg tgatgcctgc
     1501 tggagtaagt tttagagaag caactagaaa agattcaggt tataaacaaa ttgatggtaa
     1561 ttattacctt aaattaagga aacaaggtga tactgattac aattatacta ttcgtgagaa
     1621 tggtactgta tacgacgatc gtaccaacag accaactgaa ttttcagtag ataaaaattt
     1681 cggtattaaa caaaatggta attatttgac ggtagagcaa tattcggttt catttgaaaa
     1741 taataaaaag actgaatatc gtaatgggac taaagtccat atgaatatct tctacaaaga
     1801 tgcattgttt aaagtagtgc caacgaacta tattgcatat atttctagca atgatcatgg
     1861 cgaatcttgg tctgcaccaa ctttattacc tcctataatg ggacttaatc ggaatgcgcc
     1921 atatttaggt cctggacgtg gaatcattga aagctcaact ggacgtattc ttattccgtc
     1981 ttacactggt aaagagtctg cgttcattta tagtgacgat aatggagcat cttggaaagt
     2041 taaagtagtg ccacttcctt ctagttggtc agcagaagca caatttgtag aattgagtcc
     2101 aggagtaatt caagcatata tgcgtacaaa taatggtaaa attgcatatt taacaagtaa
     2161 agacgcaggt actacttgga gtgcaccgga atatttgaaa tttgtttcaa atccaagtta
     2221 tggaacacaa ttatcaatca tcaattatag ccaattgatt gatggtaaaa aggctgtcat
     2281 tttaagtact ccaaactcca caaatggtcg taaacacgga caaatttgga ttggtctaat
     2341 taatgatgat aatacaattg attggcgtta tcatcacgac gttgattata gtaactatgg
     2401 atactcatat tcaacattga cagagttacc aaatcatgaa attggattga tgtttgaaaa
     2461 atttgattca tggtctcgta atgaacttca tatgaaaaat gttgtaccat atataacatt
     2521 taagattgaa gatctgaaaa agaattaaag ctgaaatttg aaaatatata aaaagaggat
     2581 aaaaattatg gtaaattacg gtattgttgg agctggatat tttggagctg atttagctcg
     2641 ctcaatgaac aaaattgaag atgcaaaagt ggttgcggta tttgacccaa atcatggaga
     2701 agaagttgct caagagttgg gatcagatgt ttgtgcaagt ttagatgaac ttgtagcacg
     2761 tgaagatatt gattgtgtga tcgtagcttc acctagctac cttcaccgtg aaccagttgt
     2821 gaaagctgct caacatggca aacacgtatt ttgtgaaaag ccaattgcat tgtcttatga
     2881 agattgtaaa gccatggttg acgcatgtaa agaaaataat gtcatcttta tggctggtca
     2941 catcatgaac ttctttaacg gtgtacacca tgctaaagaa ttgattactc aaggtaaaat
     3001 cggtaaagtt ctttattgcc atgctgctcg tacaggttgg gaagaacaac aaccaactgt
     3061 atcatggaag aaacttcgtt ctcaatctgg aggacatttg taccaccata ttcatgaatt
     3121 agattgcatt cagtttatca tgggaggact tcctgaaaaa gcgacaatgg taggaggcaa
     3181 tgtatatcat aaaggtgaaa actttggtga tgaagatgat atgctcattg taaacttaga
     3241 atactctgat gatcgttatg ctgttttgga atatggtaat gctttccgtt ggggtgaaca
     3301 ctacgtcttg attcaaggaa ctgaaggagc tatcaaactt gacttgttca atactggcgg
     3361 tactcttcgt gttaaaggtg aaggagaatc acacttctta gttcatgaaa ctcaagagga
     3421 agatgatgat cgtacagcta tctataccgg tcgtggtatg gatggagcaa ttgcgtacgg
     3481 taaaccagga gtacgttgcc cattatggtt gcaaacatgt attgataaag aaatggaata
     3541 tctacatgac atcattaaag gtggagaaat tacagaagaa tttgaaaaac ttctcaatgg
     3601 tgtagctgct ttagaatcaa tcgctaccgc tgatgcatgt actttatcag ttaaagaaga
     3661 tcgaaaagta agtctttcag aaatcacaaa tgcttaactt ttgtaaaaca gaatagtaaa
     3721 ttcttgtcat tatataattt ctaaagttct gtgatacaac tcattgaata aagaaataga
     3781 gatgggactg ggataatgcc cagtcccatt ttttatcaaa aagtaatgag atcaaaaatg
     3841 tgggagtgtt gaaatgaaga ttataggtat cgatattggc ggaacaacaa ttaaggcaga
     3901 tttatacgat gagtttggaa cgagtttgaa tcatttcaaa gagatagaaa caattattga
     3961 ctatgatttg ggaacgaatc agatattaaa tcaggtctgt gatttaattg gtgagtatac
     4021 tttaaatcat tcaattgatg gtgttgggat ttccactgct ggagttgtta atgctaatac
     4081 tggagaaatc atctatgcag gctatacaat accagggtat atcggagtaa actttactgc
     4141 cgaaatagaa aaacgttttg ggttgtatac ttttgttgaa aatgatgtta attgtgctgc
     4201 attaggtgaa ttgtggaagg gacaagccaa agataagaaa aatgtagtaa tggttactat
     4261 tggaacaggt ataggaggca gtattattgt caacggacaa attgttaacg gatttaacta
     4321 tactgctggt gaagtaggtt atattcctgt aggtaattcg gattggcaaa gtaaagcctc
     4381 aacaaccgca ttgattcatt tatatcaaaa aaagagcttg aaaactaatc aaactggacg
     4441 tactttcttc actgatttaa gatctggaga taaagttgct gaagaaactt ttgaaatttt
     4501 tgtagaaaat ctaacaaaag gtttattaac gatttcttat ctacttaatc cagaaattct
     4561 catattagga ggtgggattc tggatagtaa ggatattttg ttacctgaaa ttcaaagttc
     4621 tttagctaaa aatgcaatgg ataataggtt tttacctaaa aatcttgtgg cagctacatt
     4681 aggaaatgaa gctggtcgta taggagctgt aaaaaatttc ttagatagaa tttctaataa
     4741 atagtatgta agataaggag gtgtcacaat gactaactct gtattttcga caatgcaaga
     4801 tattgagaat gttgcaaccg atattataaa atcatatgat aatgagattt atacttataa
     4861 agctgtttcc caagaagaat tggaaaaact agaaaaaagt tatgatgaaa aaagtcacga
     4921 agaattagtt tcaatagaaa gcaatttaga aatgaaacaa cagaacctta ttgatgaggt
     4981 taataaaaca atcaaggaaa atgatgcaaa tattcagtat atttcatcaa gtaggagagg
     5041 agaatttgta gaaaaaatta ttggtagggt ggtagaaaaa tatggccatt agtcagatga
     5101 aaagaatctc tctactattt tctaaaagta gtcttgatga tgttttaaaa actattcaag
     5161 aactagagtc agtgcagttc cgtgatttaa aggttcagga taactggtca gaagctctag
     5221 aaaaagatga agttgtattt ccaactattc aaatttttca tacttctaat tccaatcatg
     5281 gggttattga gggaaatgat gccttgactt atttgatgaa tcaacaacaa catttagaag
     5341 caactgtaga gaaattacaa gaatacctac cgaaagaaaa cacgtttaaa ttattgcagc
     5401 aacctccgat aactacctct tatgaagaat tagagaaatt tggtaaagct aatgttgctg
     5461 agggtgttct taaaaaagtg aatcatcaaa ttaacagagt tcatgaatta gaaagacaca
     5521 ttcaaagtaa taatgaggaa atagagcgat taataaagtg ggaaaaatta gaaattgttc
     5581 ctgcgaattt agaacaattt tctttctgta aaggaaaagt cggaacaatt ccaaggactg
     5641 aagataatcg cttatacaat agtcttttag aaaacaatat tgaagttcaa gaaatatttt
     5701 ctaatgatag agagtacggt gttgttgttt tctatcagtc tagttactct atagattttg
     5761 atgaatactt atttgaacca tttgattatt ctagaaagga attaccgaag cagcgagtag
     5821 tagatttaga tcaagaaaac atgcagttaa taactgaaaa agagaatatt atcgcatcgt
     5881 tgcaagattc aaagaaatat ttgatagatt tacaatggca aatagactat attttatcta
     5941 tctatgctcg tcaaatctct aagaataact ttttgtgcac tccgcatcta gttgcattag
     6001 aaggatg
//
LOCUS       DAVLWA010000100         5603 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLWA010000100 DAVLWA010000000
VERSION     DAVLWA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.100"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            102..761
                     /locus_tag="VYN80_001828"
     CDS             102..761
                     /locus_tag="VYN80_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1541694.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFIKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            786..1853
                     /locus_tag="VYN80_001829"
     CDS             786..1853
                     /locus_tag="VYN80_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1541695.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            1956..2966
                     /locus_tag="VYN80_001830"
     CDS             1956..2966
                     /locus_tag="VYN80_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541696.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2978..4669
                     /locus_tag="VYN80_001831"
     CDS             2978..4669
                     /locus_tag="VYN80_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1541697.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4672..5382
                     /locus_tag="VYN80_001832"
     CDS             4672..5382
                     /locus_tag="VYN80_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1541698.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDKIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1836 a          778 c         1071 g         1918 t
ORIGIN      
        1 ttttaaaaaa atttttgaaa gcgcttgaca aaggaaacgg tttcattgta taatgtaaac
       61 atggtgacac cgatgtcaaa tataaaaaag aaggtcttta tatgaaaaac ttaacaaaaa
      121 ttaagttcaa agagaatggg gaatttaatc atttccctgg gaatacagtt gtagcaaatc
      181 tttatactaa acaagatttg atggaagttg ttgatattat tcaatcacgt tatagagaat
      241 taccatttat cgataagttt actttaactc caaggaattc tattcacatg actgtaattg
      301 aattgttgtg ccatgaaaat cgtgaaacgg agttttggag cagtaatctt cctctagata
      361 cacctttaca ggaaatacac gattactttg ctaaacaact tgaaattttt ccattgttgg
      421 atgaagaaat tcatatgcgt gtaactgaaa tgggaaaaca aaacatacta gttgaacctg
      481 cagatgaagc ttctgcaaag agattagaag aaattcgtac ttatgtctca gaaaaagcag
      541 gtgttcgttt ccctaatcat gatagatatc aattccatat ttcaattggg tatcttcgga
      601 ttcctctaac cgaagaggag gaagaagagt ttattaaagt cagagcagaa ttaactgaaa
      661 ttttattaga gaagattcca actattactg taaaccgtat tgactatact gtatttgaag
      721 atatgagaca atttgttcca tatcacgaaa aatttaaata aaataataaa aaaggagaac
      781 ttatcatgaa aaaaatgaaa gtttggtcta ctgtacttgc aacgggagtt gctcttacta
      841 cacttgctgc ttgctctgga ggttcaaatt ctacgactgc ttcttcatct gaagaaaaag
      901 ctgataaaag tcaagaacta gtaatctatt cgaactcagt ctcaaatggt cgtggtgatt
      961 ggttaactgc taaagcaaaa gaagctggtt ttaatatcaa aatggttgat atccctggcg
     1021 ctcaattagc agaccgtgtt attgctgaga agaataatgc agttgcagat atggtatttg
     1081 gaattggtgc tgttgattca aataaaatta gagatcaaaa attactagta cagtacaagc
     1141 ctaaatggtt agataaaatt gatcaatctt tatcagataa agataattat tataatcctg
     1201 tgattgttca accattagtt ttaattgggg cgcctgatgt aaaagaaatg cctaaagatt
     1261 ggactgaatt aggtagtaag tataaaggta aatattcaat ttctggtctt caaggaggta
     1321 caggacgggc aattctagca agtatcttag ttcgatacct tgatgataaa ggtgaattag
     1381 gtgtttccga aaaaggttgg gaagtagcaa aagaatattt gaaaaatgca tacactcttc
     1441 aaaagggaga aagttcaatt gttaagatgt tagacaaaga agatccaata caatatggaa
     1501 tgatgtgggg ttctggtgca ttagttggac aaaaagaaca aaatgttgtt ttcaaagtta
     1561 tgactcctga gattggtgta ccatttgtaa ctgaacaaac tatggtttta agcactagta
     1621 aaaaacaagc gttagctaaa gaatttattg attggtttgg tcaatcagaa attcaagtag
     1681 aatatagtaa gaactttgga tctattcctg caaataaaga tgccctcaaa gatctaccag
     1741 aagatactaa gaaatttgtt gatcaagtga aaccacaaaa tattgactgg gaagctgttg
     1801 gaaagcattt ggatgaatgg gtagaaaaag ctgaattaga atacgtacaa taagaaataa
     1861 gagttaattt ttatgtccaa gtctctctat tatttctaaa tttatggaag aaataaagag
     1921 aggcttttgg tttagtaata gagggagtta gtgagatgat taaatttgat aatattcaaa
     1981 ttaaatatgg tgattttgtt gcaattgata atctgaattt agatatacat gaaggggaat
     2041 tttttacatt tcttgggcct tcaggatgtg gtaaatcaac tactttgaga gcattggtag
     2101 gttttctaga tccatcatca ggaagtattg aagttaatgg aacagatgtc actcatttgg
     2161 aacctgaaaa gcgtggaatt ggtattgtat ttcaatctta tgcgctattt ccaactatga
     2221 ctgtttttga taatattgca tttggtttaa aagttaagaa ggtagctcca gatgttatta
     2281 aagctaaagt atcagcagtg gcagcaaaaa ttaagatctc tgatcaacag ttacagcgta
     2341 atgtatcaga attatctggg ggtcaacaac aaagggtagc attggctcgt gctctggttc
     2401 ttgaacctaa aattctttgt ctagatgaac cattgtcaaa ccttgacgca aaattacgtg
     2461 tagatttgag aaaagagttg aaaagacttc aaaaagagtt aggtattact actttatatg
     2521 ttactcatga tcaagaggaa gccttgactt tatctgatag aattgcagtc tttaacaatg
     2581 gatacatcga acaggtcggt acaccagtag agatttatca taattctcaa actgaatttg
     2641 tatgtgattt tattggagat attaatgttt tgaccgatga aacagtccac gaagtattat
     2701 tgaaaaatac aagcgttttc ttagaggata aaaaaggata cattcgatta gagaaagttc
     2761 gattcaatcg tgaaactgaa caagatttta ttctaaaagg gacaattatt gatgttgagt
     2821 tttctggagt tacaattcac tatacaataa aagtttctga aagtcagatt cttaatgtaa
     2881 caagtattga tagtcaggct gctattagat ctgtcggaga aagtgtggaa ttatttatca
     2941 caccatcaga cgttctgcaa ttttaaggag gtgcagtatg cgtcataaat taaatttaaa
     3001 agattggctt attcgtttag ggttaatctg gttcttagta acatttatta tttatccaaa
     3061 ctttgatcta gtagtgaatg tatttgtaaa aggaggagaa ttttcccttg atgctgtaca
     3121 tcgtgttcta aaatctcaga gggcacttca gagtattatg aacagtttta agttagcatt
     3181 ttcactcatt attacagtta atgtcgtagg tattctttgt gttctattta cagagtactt
     3241 tgatattaaa ggtgctaaaa ttttaaaatt aggttatatg acctctttaa tttatggagg
     3301 agtggtttta gcgactggat ataaatttgt ctatggtcct tatggattga ttacaaaatt
     3361 tttacaaaat gttatccctt ctttagaccc taactggttt attgggtatg gtgcagtctt
     3421 attcattatg acattttcag gaactgctaa tcatacattg tttttaacaa atacaattcg
     3481 aagcgttgac tatcacacta ttgaggctgc tcgaaatatg ggagcaaaac catttactgt
     3541 tttccgaaaa gtagtgttac caaccttaat tccaactcta tttgcactta ctattatggt
     3601 ttttcttagt ggtttatctg cagtagcagc acccatgatt gttggtggta aagaatttca
     3661 aactataaat ccaatgatta ttacatttgc agggatgggg aattctcgtg atttagctgc
     3721 cttacttgca attattttag gtattgcaac tacaattttg cttactatca tgaataagat
     3781 agaaaaaggt ggaaattata tttctatctc taagactaaa gcgcctctta aaaaacaaaa
     3841 aattgcgtct aagccttgga atatcattgc tcacattgta gcatatggat tgttcacagt
     3901 tttcatgctt ccactaattt ttatagtatt atactcattt acagatccag ttgcaattca
     3961 aacaggtaac ttaacattat caaactttac tttagaaaat tatcgcttat tctttagtaa
     4021 tagtgcggca ttctctccat tcttggtcag ctttatttat tctattattg ctgcgacaac
     4081 agcaacaatt ctcgcagttg tatttgctcg tgttgtcaga aaacataaat ctcgttttga
     4141 tttcttattt gaatatggtg ctctacttcc ttggttacta ccaagtacac ttttagcagt
     4201 aagtttatta tttactttta atcagccaca atttcttgtc ttgaatcaga ttttggtagg
     4261 tagtttggta attctactta ttgcatatat agttgtaaaa atcccatttt cttatagaat
     4321 ggtacgtgct attttattta gtgttgatga tgagatggaa gatgcagcaa gaagtatggg
     4381 tgcttcacct ttttatacta tgatgaaggt tatcattcca tttattttac cggttgttct
     4441 ctctgttatt gctttaaact ttaactcttt attaactgac ttcgacttat ctgtattcct
     4501 ttaccatccc ctagctcaac cattaggtat tacgattcga tctgcaggtg atgaaacagc
     4561 aacatctaat gcacaagctc tggtatttgt ttatacaatt gttctgatga ttatttctgg
     4621 aacggtatta tacttcacac aaagaccggg gcgtaaagta aggaaataat catgacagcc
     4681 actagtcttg ggttatcaaa tattgaaata gttgtcagga ttgttttatc agtagtcatt
     4741 ggtagtataa ttggtttaga gagagggagc aaatcccagc ctgcaggcat ccgaacttat
     4801 agtattgttt gtctagctgc atgtttgatt atgatgacga atgaatacgt atcttataaa
     4861 tttgggacag gagatcctac acgattagga gctcaagtta tatcaggtgt gggttttcta
     4921 ggcgctggaa cgattcttat tacagataaa aagaaaatta caggtctgac aactgcagca
     4981 ggcatttggg cttcggcagg aattggatta gctattggag taggttttta tgagggagct
     5041 cttttagtag ccatttctgt ttggggtgtg atatccatgt tccaaccact aaaaaaatat
     5101 ctgcaaaatc gttctaaaat gattgaattg tatatagtgg ttaaatctac agaagcctat
     5161 aatcgtgtat tggtttattg tgcagaaaat ggtatcagaa tgaccgattc aagaacagcg
     5221 tttggagatg ttaattctga caaaattgaa tactttgatg ttccggacaa aaaaatagcg
     5281 tcatttatta ctctggaatt atcaggtaga tttgaacatc ttcgactgat ggaagaaatt
     5341 gctaatattg taggtgttat ttatgtcgaa gaaattagct gaaatttagt aaacaaaggg
     5401 acagatgttt ttgtcctttt gttttttaga tgttttaatc atctaatttt tgagtagatt
     5461 tttgctcaga ggtttttatg aaagattata aaatcaattt tgatttaggt aaaatagaat
     5521 attttgataa taattgtttg atacaagtat acaaatttat ttctttttat gatatttgtg
     5581 aaatggtgtt tgcctttcat tta
//
LOCUS       DAVLWA010000101         5420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLWA010000101 DAVLWA010000000
VERSION     DAVLWA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.101"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..200)
                     /locus_tag="VYN80_001833"
     CDS             complement(<1..200)
                     /locus_tag="VYN80_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541699.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTT"
     gene            370..642
                     /gene="rpsP"
                     /locus_tag="VYN80_001834"
     CDS             370..642
                     /gene="rpsP"
                     /locus_tag="VYN80_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1541700.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            662..901
                     /locus_tag="VYN80_001835"
     CDS             662..901
                     /locus_tag="VYN80_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1541701.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            1169..1373
                     /locus_tag="VYN80_001836"
                     /pseudo
     CDS             1169..1373
                     /locus_tag="VYN80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            1477..2265
                     /locus_tag="VYN80_001837"
     CDS             1477..2265
                     /locus_tag="VYN80_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1541702.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            2296..2814
                     /gene="rimM"
                     /locus_tag="VYN80_001838"
     CDS             2296..2814
                     /gene="rimM"
                     /locus_tag="VYN80_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1541703.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            2804..3523
                     /gene="trmD"
                     /locus_tag="VYN80_001839"
     CDS             2804..3523
                     /gene="trmD"
                     /locus_tag="VYN80_001839"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1541704.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGSQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKILVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            3535..3873
                     /locus_tag="VYN80_001840"
     CDS             3535..3873
                     /locus_tag="VYN80_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541705.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(3904..4224)
                     /locus_tag="VYN80_001841"
     CDS             complement(3904..4224)
                     /locus_tag="VYN80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541706.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            4322..4537
                     /locus_tag="VYN80_001842"
     CDS             4322..4537
                     /locus_tag="VYN80_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1541707.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(4606..4686)
                     /locus_tag="VYN80_001843"
                     /pseudo
     CDS             complement(4606..4686)
                     /locus_tag="VYN80_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4742..5278)
                     /locus_tag="VYN80_001844"
     CDS             complement(4742..5278)
                     /locus_tag="VYN80_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1541708.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         1796 a          928 c         1141 g         1555 t
ORIGIN      
        1 gtggtaaaag taccaaactg gattctccat agtaaaatga tatctcctgc tagtgaatca
       61 tctactaata agtccatatt tcgcttggga acgggcttgg gacttagaag ccaagtatcc
      121 aactctcgtt caggcgatat gaagggtttg acttggtgtt ctttataaaa tttttctaaa
      181 atagtattca cggatagcat ctttcaaaca tttatttcct attctaacaa atttctaaat
      241 actttaagaa atttatagtc tgtcaagttt ttttcttgac acatgccaga aatctgctat
      301 aatagaacat gtgctagata gctcagctat ttcaccgaaa taaaaaatta aagaaatgag
      361 actttaaaaa tggcagttaa aatccgtttg actcgtatgg gttctaagaa aaaacctttc
      421 taccgtatca acgtagcaga ttcacgttca ccacgtgacg gacgtttcat cgaaacagtt
      481 ggaacttaca acccacttgt tgctgaaaac caagtaactt tgaaagaaga ccgcgttctt
      541 gcatggttgg ctaatggagc tcaaccttca gacacagtac gtaacatcct ttcaaaagaa
      601 ggcgtattga aaaaattcca cgattctaaa ttttcaaaat aagtttaaag taggttgaca
      661 gatggatacg attgaaaatc tcattattgc gattgtgaaa cccttaattt cacaaccaga
      721 tgccttaact atcaagattg aggatacacc agaatttttg gaatatcatt tgaatcttga
      781 tcaaagcgat gtgggtcgtg taatcggtcg taagggtcgc actatttctg cgataagaac
      841 gattgtctac tctgtcccaa ctgaatacaa aaaagtaaga atcgttattg acgaaaaata
      901 agaagggcgg gacggatgtc tcgctttttt gtgtggaata gaagatatag taaaatgaaa
      961 taataacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagta
     1021 aaggtgtact attctagttt caatatacta taaaagattg aacgtttagg caattccaag
     1081 actacttact cgaacattaa cagcagtctc ggactgatga aggtctcttt atgaagttag
     1141 tggagaagtc ggagaagtag ccgaggtttt gaataggcgc tctggtcgaa aagaagggat
     1201 aggattcgaa cgaggaactt gccagagaac tggctgatat catttactac accgtcgcaa
     1261 tcgtagctat taatcatatt gaccttacca agactgtctt tgagaaagac aaaaccgcag
     1321 ccgttaatta ccaacataaa catgatttgg aaggattttt gaagggaaag tagagatgtt
     1381 gtccccttcc tccaacttga gaaaatcctt ggaacgtgat aaaataaaag ataaggattt
     1441 ttaataattc attatataga atgagtggat ttctttatga agaggtttga agtatctaca
     1501 gaaattggta gcctctctgt tacttatcaa aagcaaaaga aagtgctagt ttgtctaaac
     1561 ggagcaggtt tgctaccaag ttatgaaaat ttttcactta tacttgaaaa acctcctcct
     1621 acaattggtt atttgacaat tgattttccg aacacaggta ggagtccgat tcatgaccaa
     1681 gctggaaaaa atctagataa tcttgcagat gtggtttatg aagtacttga agaattaggg
     1741 atttctgaat atatactttg tgcacatagt tggagtggaa ttttagcttg caaattactc
     1801 gagaaaccaa ttaagtgtca gactttagta gcaattgaac cgacaactaa aaaagtcatg
     1861 tttgctgatt tttcagaaaa tccttatcca gaaatggaag agcagatgag gctgattgac
     1921 gagtgtggtc ctgaacttta ttttaagaac ttaactcaag caacatttag tcctgaaacg
     1981 aataaaaaaa tctgggaatt aatgcaagaa aaaggcttag agttggaaaa tcaagatcca
     2041 gaatttcaga tatctggaga gattactgag gaagattttg agaatttgtc gatagaatct
     2101 catgtccctg tatttatttt ttgtcagact tatagagaaa aagagtacag agaatcagaa
     2161 tattggactt ctaatactaa actcatttta ggagggaatc accattattt acaatggtca
     2221 gaatcggaaa aaattgcggc tattattcga gaattgtcag aataagatgg aaaaaaggag
     2281 attacaggag acaagatgaa ctactttaat gttgggaaaa tcgttaatac gcagggatta
     2341 cagggtgaga tgcgagtctt gtctgtgacg gattttgcag aagaacggtt taaaaaagga
     2401 gctgagctgg ctttgtttga tgaaaaagat cagtttgtcc aaacagtgac catcgctagc
     2461 caccgtaaac agaagaactt tgacattatt aaattcaaag atatgtacca tatcaatact
     2521 atcgaaaagt acaagggata tagtctcaag gtcgctgagg aagatttgaa tgacctagac
     2581 gatggtgaat tttactatca cgagattatc ggtttggaag tctatgaggg tgatagcttg
     2641 gttggaacca tcaaggaaat cctgcaacca ggtgctaatg atgtctgggt ggtcaaacga
     2701 aaaggcaaac gtgatttgct tttaccttat atcccaccag tggttctcaa tgttgatatt
     2761 ccaaataaac gggtcgatgt ggaaatctta gaagggttag acgatgaaga ttgatatttt
     2821 aaccctcttt ccagagatgt tttctccact ggagcactca atcgttggaa aggctcgaga
     2881 aaaagggctc ttggatatcc agtatcataa ttttcgagaa aatgctgaaa aggcccgtca
     2941 tgtagatgat gagccctacg gaggcagtca aggcatgttg ctcagagcac aacctatttt
     3001 cgattccttt gatgctattg aaaagaaaaa tccgcgcgtt attctcctcg atcctgctgg
     3061 aaagcagttt gatcaggctt atgctgaaga tttggctcaa gaggaagagc taatctttat
     3121 ctgtgggcac tatgagggtt atgatgagcg cattaagatc ttggtaacag atgagatttc
     3181 cctaggcgac tatgtcctca ctggtggaga attggcagct atgaccatga ttgatgctac
     3241 agttcgcctg attccagaag tgattggcaa ggagtctagc caccaagatg atagtttttc
     3301 ttcaggtctt ttagaatatc ctcagtacac acgtccctat gattatcgag gcatggtcgt
     3361 gccagatgta ttgatgagtg gccaccatga aaagattcgt cagtggcgat tgtacgagag
     3421 tttaaagaaa acctacgagc gcagaccaga tttacttgaa cattatcaac tgacagtaga
     3481 agaagaaaaa atgctggcag aaatcaaaga aaacaaagaa taaaggagaa acctatgcaa
     3541 gtaatcaaac gtaatggcga aattgctgaa tttaatccag ataagattta ccaagccatc
     3601 ttgaaggcag cccaaactgt ttatgttttg acagatgatt tgcgtcaaaa tcttgctcaa
     3661 gtcactaaga aggtagtttt ggatttacaa gaagccaagg tggaacgtgc gactatcagt
     3721 atgattcaat ctatggttga acatcgttta ttgggcgcag gttacattac cattgcagaa
     3781 cactacattt cctatcgttt acaacgtgac ttggaaagaa gtggttatgg agatcatatt
     3841 gcagttcatt tacattttga acaaattcgc taagaaaaaa gagtgggatg aagcaactac
     3901 atctcactct ttcttaaatc tatttttttg ggctgtttgg acagttgagg ggacaaatcg
     3961 caggcgttga atatcgctta tacggataat ccggctcaca tttttggcaa aatttttcac
     4021 gataatttgc tggccttgct gatcatattt gatgatatca cctgtaaaac ttgtctcaga
     4081 caagataagg tgaacagcgg tttgtttctg gatagcctct tcaatagtgg ctgtaaggga
     4141 gttggtttca gtctggtcag gttggtcatg tccatttaaa aaatcatgta ttttatctag
     4201 aacttgctgg aatttatgtc tcatattggc ctcccttctt ttatatcaat attatataaa
     4261 attttcctaa aatctacaag attttacgaa taaactaatg aaactaaaaa aggagaagaa
     4321 aatggcagaa tttacatttg aaatcgaaga gcacttgttg actctttctg aaaacgaaaa
     4381 aggttggacc aaggaaatta accgtgtgag ctttaatggt gcccctgcaa agtttgatat
     4441 tcgtgcttgg agtccagacc atactaaaat gggcaaaggg attactctct caaatgaaga
     4501 atttcaaacg atggtggatg cctttaaagg caactaatac tcttcgaaaa tctcttcaaa
     4561 ccgcatcagc tttgccttgc cgtatgtatg gttactgact tcgtcagttt catccacaac
     4621 ctcaaaacag tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
     4681 tgagtataag ttttcaaaat aaaaaaaagg atattgagtc atgataactc ggtatccttt
     4741 tttagttagt aaagtaagcc tgatttttaa ggcgttgaag taggggacgg ctaataaaac
     4801 taatagccag aagtttgcca aggtctggaa tgataaaggg aagaaccccc acaacaagag
     4861 ctttttcaaa tgccattcca gctaggaaat gcaagctgag aatcccgccg acaaagacaa
     4921 gggcatcacc caagaggttt gcaagaaaaa tcttaacaac accactcttg ctgttggtta
     4981 gagaggaagt aagtccagag taaacgagat aaaaccaaag atagcctgca gtagggccaa
     5041 ctaaagcctg aaaaccagct ccacctcctg caaagacagg aagaccgata gcacctagaa
     5101 gaagatagag tccagcagaa agtacagcct ctctcggtct aaagacagta gcaatcaagc
     5161 cgattgcaaa gttttgcaga gtgaagggaa caggtccaat tggaagactg atttgtgcca
     5221 aaacagcaat gagagcagcc ccaatagcag ggatagcata aacgtgagct tttttcaaaa
     5281 tagttaacct cttttctaat gtatagtaac tattatactc atataggagt cgatgtcaac
     5341 cattttttaa aaacaaggta acaaaaactg taacagccga ttttcgtgac tagtcaagtt
     5401 ggataatttt tttgttgatt
//
LOCUS       DAVLWA010000102         5381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLWA010000102 DAVLWA010000000
VERSION     DAVLWA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.102"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            71..>5381
                     /locus_tag="VYN80_001845"
     CDS             71..>5381
                     /locus_tag="VYN80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1541709.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEF"
BASE COUNT         2125 a          757 c         1049 g         1450 t
ORIGIN      
        1 ttataacaaa ggctagcaaa gttaaagttt attatctatt ggaagttaaa taaatatgta
       61 aggaattaaa atgaaaaaaa gtacagtatt gtcattaacc acagctgcag ttattttagc
      121 agcctatgcc cctaatgagg tagtcttagc agacacatct agctctgaag atgctttaaa
      181 catctctgat aaagaaaaag tagtattaga taaggaaaca gaaaataaag agaaacatga
      241 agatattcat aatgctatag aaacttcaaa ggatactgaa gaaaagaaaa caacagttat
      301 tgaggaaaaa gaagttgtta gtaaaaatcc tgtgatagac actaaaacta gcaatgaaga
      361 agcaagaatc aaagaagatt ccaatcaatc ccaaggagat aatgcacact cgtctgcaaa
      421 taaaggcaca gaaaatccga aaaaagaaga tagacttgtc tatattgctg aatttaaaga
      481 taaagaatct ggagaaaaag caatcaagca actatccaat cttaagaata caaaagtttt
      541 atatacttat gatagaattt ttaacggtag tgccatagaa acaactccag ataacttgga
      601 caaaattaaa caaatagaag gtattacatc ggttgaaagg gcacaaaaag tccaacccat
      661 gatgaatcat gccagaaagg aaattggagt tgaggaagct attgattacc taaagtctat
      721 caatgctccg tttgggaaaa attttgatgg tagaggtatg gtcatttcaa atatcgatac
      781 tggaacagat tatagacata aggctatgag aatcgatgat gatgccaaag cctcaatgag
      841 atttaaaaaa gaagacttaa aaggaactga taaaaattat tggttgagtg ataaaatccc
      901 tcatgcgttc aattattata atggtggcaa aatcactgta gaaaaatatg atgatggaag
      961 ggattatttt gacccacatg ggatgcatat tgcagggatt cttgctggaa atgatactga
     1021 acaagatatc aaaaacttta acggcataga tggaattgca cctaatgcac aaattttctc
     1081 ttacaaaatg tattctgacg caggatctgg gtttgcgggt gatgaaacaa tgtttcatgc
     1141 tattgaagat tctatcaaac acaacgttga tgttgtttcg gtatcatctg gttttacagg
     1201 aacaggtctt gtaggtgaga aatattggca agctattcgg gcattaagaa aagcaggcat
     1261 tccaatggtt gtcgctacgg gtaactatgc gacttctgct tcaagttctt catgggattt
     1321 agtagcaaat aatcatctga aaatgaccga cactggaaat gtaacacgaa ctgcagcaca
     1381 tgaggatgcg atagcggtcg cttctgctaa aaatcaaaca gttgagtttg ataaagttaa
     1441 cataggtgga gaaagtttta aatacagaaa tataggggcc tttttcgata agaataaaat
     1501 cacaacaaat gaagatggaa caaaagctcc tagtaaatta aaatttgtat atataggcaa
     1561 ggggcaagac caagatttga taggtttgga tcttaggggc aaaattgcag taatggatag
     1621 aatttataca aaggatttaa aaaatgcttt taaaaaagct atggataagg gtgcacgcgc
     1681 cattatggtt gtaaatactg taaattacta caatagagat aattggacag agcttccagc
     1741 tatgggatat gaagcggatg aaggtactaa aagtcaagtg ttttcaattt caggagatga
     1801 tggtgtaaag ctatggaaca tgattaatcc taataaaaaa actgaagtca aaagaaataa
     1861 taaagaagat tttaaagata aattggagca atactatcca attgatatgg aaagttttaa
     1921 ttccaacaaa ccgaatgtag gtgacgaaaa agagattgac tttaagtttg cacctgacac
     1981 agacaaagaa ctctataaag aagatatcat cgttccagca ggatctacat cttgggggcc
     2041 aagaatagat ttacttttaa aacccgatgt ttcagcacct ggtaaaaata ttaaatccac
     2101 gcttaatgtt attaatggca aatcaactta tggctatatg tcaggaacta gtatggcgac
     2161 tccaatcgtg gcagcttcta ctgttttgat tagaccgaaa ttaaaggaaa tgcttgaaag
     2221 acctgtattg aaaaatctta agggagatga caaaatagat cttacaagtc ttacaaaaat
     2281 tgccctacaa aatactgcgc gacctatgat ggatgcaact tcttggaaag aaaaaagtca
     2341 atactttgca tcacctagac aacagggagc aggcctaatt aatgtggcca atgctttgag
     2401 aaatgaagtt gtagcaactt tcaaaaacac ggattctaaa ggtttggtaa actcatatgg
     2461 ttccatttct cttaaagaaa taaaaggtga taaaaaatac tttacaatca agcttcacaa
     2521 tacatcaaac agacctttga cttttaaagt ttcagcatca gcgataacta cagattctct
     2581 aactgacaga ttaaaacttg atgaaacata taaagatgaa aaatctccag atggtaagca
     2641 aattgttcca gaaattcacc cagaaaaagt caaaggagca aatatcacat ttgagcatga
     2701 tactttcact ataggcgcaa attctagctt tgatttgaat gcggttataa atgttggaga
     2761 ggccaaaaac aaaaataaat ttgtagaatc atttattcat tttgagtcag tggaagaaat
     2821 ggaagctcta aactccaacg ggaagaaaat aaacttccaa ccttctttgt cgatgcctct
     2881 aatgggattt gctgggaatt ggaaccacga accaatcctt gataaatggg cttgggaaga
     2941 agggtcaaga tcaaaaacac tgggaggtta tgatgatgat ggtaaaccga aaattccagg
     3001 aaccttaaat aagggaattg gtggagaaca tggtatagat aaatttaatc cagcaggagt
     3061 tatacaaaat agaaaagata aaaatacaac atccctggat caaaatccag aattatttgc
     3121 tttcaataac caagggatca acgctccatc atcaagtggt tctaagattg ctaacattta
     3181 tcctttagat tcaaatggaa atcctcaaga tgctcaactt gaaagaggat taacaccttc
     3241 tccacttgta ttaagaagtg cagaagaagg attgatttca atagtaaata caaataaaga
     3301 gggagaaaat caaagagact taaaagtcat ttcgagagaa cactttatta gaggaatttt
     3361 aaattctaaa agcaatgatg caaagggaat caaatcatct aaactaaaag tttggggtga
     3421 cttgaagtgg gatggactca tctataatcc tagaggtaga gaagaaaatg caccagaaag
     3481 taaggataat caagatcctg ctactaagat aagaggtcaa tttgaaccga ttgcagaagg
     3541 tcaatatttc tataaattta aatatagatt aactaaagat tacccatggc aggtttccta
     3601 tattcctgta aaaattgata acaccgcccc taagattgtt tcggttgatt tttcaaatcc
     3661 tgaaaaaatt aagttgatta caaaggatac ttatcataag gtaaaagatc agtataagaa
     3721 tgaaacgcta ttcgcgagag atcaaaaaga acatcctgaa aaatttgacg agattgcgaa
     3781 cgaagtttgg tatgctggcg ccgctcttgt taatgaagat ggagaggttg aaaaaaatct
     3841 tgaagtaact tacgcaggtg agggtcaagg aagaaataga aaacttgata aagacggaaa
     3901 taccatttat gaaattaaag gtgcgggaga tttaagggga aaaattattg aagtcattgc
     3961 attagatggt tctagcaatt tcacaaagat tcatagaatt aaatttgcta atcaggctga
     4021 tgaaaagggg atgatttcct attatctagt agatcctgat caagattcat ctaaatatca
     4081 aaagcttggc gagattgcag aatctaaatt taaaaattta ggaaatggaa aagagggtag
     4141 tctaaaaaaa gatacaactg gggtagaaca tcatcatcaa gaaaatgaag agtctattaa
     4201 agaaaaatct agttttacta ttgatagaaa tatttcaaca attagagact ttgaaaataa
     4261 agacttaaag aaactcatta aaaagaaatt tagagaagtt gatgatttta caagtgaaac
     4321 tggtaagaga atagaggaat acgattataa atacgatgat aagggaaata ttattgctta
     4381 tgacgatggt agtgccttac aatatgaaac tgaaaaactt gacgaaataa aatcaaaaat
     4441 ttatggcgtt ctaagcccat ctaaagatgg acactttgaa attcttggaa agataagcaa
     4501 tgtttctaaa aatgccaagg tatattatgg aaatagctat aaatcgatag aaatcaaagc
     4561 gactaagtat gactcacatt caaaaacgat gacatttgat ttatacgcta atattaatga
     4621 tattgtggat ggattagctt ttgctggaga tatgcggttt tttgttaaag atgataatcg
     4681 aataaaagct gaaactaaaa ttagaatgcc tgaaaaaaat aaggaaacta aagcagaata
     4741 tccctatgta tcaagttatg ggaatgtaat cgaattagga gaaggagatc tttcaaagaa
     4801 caaaccagac aatttaacta aaatggaatc tggtaaaatc tattctgatt cagaaaaaca
     4861 acaatatctg ttaaaggata acatcattct aagaaaaggc tatgcactaa aagtgactac
     4921 ctataatcct ggaaaaacgg atatgttaga aggaaatgga gtctatagca aggaagatat
     4981 agcaaaaata caaaaggcca atcctaatct aagagtcctt tcagaaacaa caatttatgc
     5041 tgatagtaga aatgttgaag atggaagaag tacccaagct gtattaatgt cggctttgga
     5101 cggctttaac attataaggt atcaagtgtt tacatttaaa atgaacgata aaggggaagc
     5161 tatcgataaa gacggaaatc ttgtgacaga ttcttctaaa cttgtattat ttggtaagga
     5221 tgataaagaa tacactggag aggataagtc caatgtagaa gctataaaag aagatggctc
     5281 catgttattt attgatacca aaccagtaaa cctttcaatg gataagaact actttaatcc
     5341 atctaaatct aataaaattt atgtacgaaa tccagaattt t
//
LOCUS       DAVLWA010000103         5320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLWA010000103 DAVLWA010000000
VERSION     DAVLWA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.103"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..977
                     /locus_tag="VYN80_001846"
                     /pseudo
     CDS             1..977
                     /locus_tag="VYN80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1003..1647
                     /locus_tag="VYN80_001847"
     CDS             1003..1647
                     /locus_tag="VYN80_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1541710.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1689..3677)
                     /locus_tag="VYN80_001848"
     CDS             complement(1689..3677)
                     /locus_tag="VYN80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541711.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3679..4437)
                     /locus_tag="VYN80_001849"
     CDS             complement(3679..4437)
                     /locus_tag="VYN80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541712.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGIFYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4865..5056
                     /locus_tag="VYN80_001850"
     CDS             4865..5056
                     /locus_tag="VYN80_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541713.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1665 a         1056 c         1042 g         1557 t
ORIGIN      
        1 caaaagccaa acatttgaca atcgatagcc gtcgcttaat cgaaagatgg aaaaaagaag
       61 gaaaatcaaa tagagaaatt gcctctctac ttggaaaagc tcctcaaact atccacactg
      121 aaatcaagcg taggacagtc cgacaatgtc ttggaaaagg gcgcttcaaa gaggtttatt
      181 ctgccgacta tgctcaacag tcttatgaaa ataatcgcaa gcactcggtc aagagatcaa
      241 gcgtgaccaa ggaactaaag gaaaagattc tccactatca taaccaaaaa ttttcgcctg
      301 aaatgatggt tatggctaaa ggggttaacg tgggaatttc aaccatttac tattggattc
      361 atcatggaaa attggggtta agcaagcagg atctgcttta ccctagaaaa ggaaaagcgc
      421 ttaagaaaca ggctagcacc aactttaaac ctgctggtca atccatcgaa cagcggcctg
      481 aagctatcaa tcttcgcttg gagaatgggc attatgagat tgatacggtt ctacttacga
      541 gatcgaaaaa ctactgcttg cttgtcttga cggatcgaaa gagtagacat cagatcatcc
      601 gattgattcc aaataaaagt gctgaggtgg tcaatcaggc tctaaaactc atcttaaaac
      661 aacacaagat tctttccatc acggcagata atggaacgga attcaatcgc ttgtttgata
      721 tattttctga ggagcacatc tattatgcgc acccctatgc ctcttgggaa aggggaacta
      781 atgagaatca caacaggctc attcgtagat agttacctaa gggaaccaag aaaatgactc
      841 ccaaagaagt cgcattcatc gaaaagtgga ttaacaacta tcctaaaaaa tgcttggact
      901 acaagtcacc cagagaagac ttctggatgg ctaacttgaa cttgaaattt agcgtcagaa
      961 acaaatcacg aaattgattc aaattttgca ggtcgtttaa atatcctgcg tgcgggtgtt
     1021 cttgatgcta acgatggaat tatttccatt gctggtgtgg ttatcggagt tgccagtgcc
     1081 acgaccaata tctggattat ctttttatca ggttttacgg ctatcttagc tggtgccttt
     1141 tcaatggctg gtggagaata tgtatccgtt tcaactccaa aagataccga ggaagctgcc
     1201 gtttcgcgag aaaaactctt gctagaccaa gatagggaac tagccaaaaa atccctctat
     1261 gctgcttata tccaaaatgg agaattcaaa acttctgccc aactcttgac caataagatc
     1321 tttcttaaaa atccactcaa ggctctggta gaggaaaaat atgggattga gtatgaagaa
     1381 tttaccaatc cttggcacgc tgccatttct agcttcgttg cctttttcct tagaagtttg
     1441 cctccaatgc tgtcagtgac catttttcca agtgattacc gcatccctgc taccgtcctt
     1501 attgtcggtg tggcccttct tctcactggt tacacaagtg ctagacttgg aaaagcccca
     1561 accaaaacag ctatgattcg gaaccttgct attggtctct tgaccatggg agttaccttc
     1621 ctgctcgaac aacttttcag catttagaat acaagaaata cctcgatttt gaagtcgagg
     1681 tatctttttt acatttgcac aatcttgcga taacttcttg aagtaatcat gaaaatcagc
     1741 acataggcga tgaggaagat agcgcagata gacaaggtca caatcaacat catagtcgta
     1801 tccagtacac caatcacttt taaaatcagg ctaagcatat ggtaggcaaa ggcgagatgt
     1861 atgaaggcaa agagcaaagg aaggaagaaa acagttaaaa cctgtttgtt gatggtttgc
     1921 ttgatttgct tttggtccaa accgactttc tgcaagataa taaagcgttc acggtcttcg
     1981 tagccttcag aaatttgttt gtagtagatg accagaacag ttccgaccat aaagataatg
     2041 gataggaaaa taccgataaa gaagacacca ccaaagaggg cactcatctg agaactagca
     2101 tctgctagat tgctaccata aacatagcta ccttctgtgt ctaattgagc attaaattga
     2161 ttgaggtagt tttcatactc ctcagcgacc ttgagttgtt cttcttcgct ggcatctaca
     2221 ttcataccac cgtaaaactg attatagata gccgaatctg ggaattgatc caaaaaggct
     2281 tgtaaatcag gaacaacaag gtaattgtaa tcagtagtca agatattaaa cttatttgga
     2341 acatggttga caataaaatc tttagtaaat tcttctttga cagaaaattg atgatcattt
     2401 agagttagag ctttctgtcc tttcagtccg tcatttttgg caaagagacc gacctcattt
     2461 cctgatagag acagtttttg accagtcata ttttcataat ctttttggtc aaataccatg
     2521 aaaactgttt ttggttggac acggttttgt cctttttcaa aaatagttaa cttggttcct
     2581 tcttgattcg caacagcaaa gtaagtgtaa cgaagcactt ctttctcttt aatcttataa
     2641 cctttgtcac ttgcaaactg gctcaagagt ttgtccaaat cttctttttc aacattttgc
     2701 cctgaaaccc caaaatcatg aggatttaga acttttttaa agctttctga ggaattgaaa
     2761 atgcttgtcg ctgctgacat ggttaccaaa accattgttg acaagatagc gatggtggct
     2821 agtccaactg catttttctt catacggaaa atcaagttag aaacagatat gagattattt
     2881 ggttggtaat agtatttctt atttttcttt aagatttgga ggaaaacggt aatccctgca
     2941 ttaaacaaga gataagtccc aaagataacc agtaaaacag ctatgaagaa ggttgttaag
     3001 gctgtaagag gatcttttac cgtaagggca agataatagc caattcctaa acttatggaa
     3061 ccaagaatgg tttggagagg aaggaagcga ccttttttct ctccactagc tttctcacga
     3121 gagagctgga gggcattcat acgggcgatt cgaagggcat tcaggaacat gaggcctagg
     3181 aaaatcaaac cgaagacaac aagcactgta atgacaactt tcgtctggaa ggtagcaacc
     3241 agctcaacct tcaatttcat tagtttgagc aggaaagcga aaattaactt gtcaaacaag
     3301 gctccaatac cgatacccgc tccaacagtt agaatcccaa ataccactaa ctccttaaag
     3361 gtcatactga taagatgacg cttctccaag cccaacatgc cataaattcc tagttccttg
     3421 gaacggttct tcatgacaaa actattggca tagagaacga taatggctga cgcaagggtg
     3481 acgacaaaca taccaaatcc aagtgtagcc tgaatggttg ttcctccacg gatttccgca
     3541 atcttaggat tgaaggttag agagtaaaag agataggtga cagtgactgc caagagaaca
     3601 gccagcgcaa aaggatagta gagtttgcgg tttttaatca agttcgatac cgctaactta
     3661 ttggttaatc gaaacatact aattcacctc gcttgccatg acagtcaagg tatcagagat
     3721 ttcttggaac atctgacgtt ctgtcttctc tccacggtag atttgattgt aaaaaatgcc
     3781 gtctttgata aagagtacac gtttagctct gctagctgct gctgttgagt gggttaccat
     3841 gagaatggtt tggccgcgct cattgatttc atcaaagaca tcaagtaagg ctgcagatga
     3901 cttggagtca agagctcctg tcggttcgtc cgcaaggaga atttcaggtt cagtgatgat
     3961 ggcgcgtgct actgctacac gctgtttctg gccaccagaa atctcgtaag ggtacttctc
     4021 ttgtaattgg tttattccca gattctcagc tgtcaccacc aatttcttca tcatctctgt
     4081 aataggtctt cttgacaaga caagcggaag caagatattg tccttaacag acagagtatc
     4141 tagcaagtta aagtcttgga agacaaatcc tagcttttca cgccggaaac tagaagcctg
     4201 tgaattttta atagttgctg tatcagttcc gtttaggtaa acctgcccac gacttggttt
     4261 atccagcata gctaggatat tgagcagagt ggatttacca gaaccagact cacccatgat
     4321 agcaacgtag tcaccctttt ctacggtgaa gtgaatatcc ttgagggcct ctacttggtt
     4381 gccctgaaaa cgagttttat aaattttttg aacgtgtttt acatctaaaa gtgtcatgag
     4441 aatctccttt cttaatatcc catcaaatga aatgtagctt gcatcatagt gcatcccagc
     4501 tgaatgcgct caatttcttt gtgacttggt tttctagttt tcttatgtaa gatttgtttc
     4561 atgatgttac tcctttgttc tttatgagtc tagtttacat caaaaaaaga ccgcttgcca
     4621 taacctaacc ttacaaaaga gactttaatc ttacattttt gtaagattgg gtgaaatata
     4681 agcaaaatta cttagaaaat gatttgattt cctattttta aagttgtaaa atataagtat
     4741 gaaaaggctt actttaaaaa ggaggaagcg ggaactaaca ttttagtaag gttacctaga
     4801 taacatgcca ttcaatttaa cacttgcaag tcgttcaacc tataaggaaa gaaggtgcgc
     4861 tttaatgaaa cataaagaac atatactcat cggatttctc tatctgctct ctccattcat
     4921 cggtcagctc ttagttgaac acacacactt tatcagtaca gaatttaccg gcactgctta
     4981 tgttatttgc tggttatcag ttgttatcag catccaccat ttttccaaaa atgtattgtc
     5041 tcaacagcaa aaatagatta aaaaactgcg tttaaattac tgcttttata gtaaaatgaa
     5101 ataagaacag gacaaatcga tcaggacagt caaatcgatt tctaacaatt ttttagaagt
     5161 ataggtgtcc tattctagtt ccaatccact atagtttgtg gatgaagctg gcgaaatatt
     5221 attaatacta ttcaaaaatc aaagtgcaaa ctatatcaac ttcacattgc cgactaaatt
     5281 acagcctgcg gctggcttcc tagtttgcac tttgattttt
//
LOCUS       DAVLWA010000104         5300 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLWA010000104 DAVLWA010000000
VERSION     DAVLWA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.104"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..121
                     /locus_tag="VYN80_001851"
     CDS             <1..121
                     /locus_tag="VYN80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1541714.1"
                     /translation="VEDARDRRGMRLTKSQVIELLLNTVDYFDDIMGAIYSKK"
     gene            183..941
                     /locus_tag="VYN80_001852"
     CDS             183..941
                     /locus_tag="VYN80_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1541715.1"
                     /translation="MVVYIRQSKLLSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIDVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            999..1853
                     /locus_tag="VYN80_001853"
     CDS             999..1853
                     /locus_tag="VYN80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1541716.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKDYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            1965..2849
                     /locus_tag="VYN80_001854"
     CDS             1965..2849
                     /locus_tag="VYN80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1541717.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3012..4751
                     /locus_tag="VYN80_001855"
     CDS             3012..4751
                     /locus_tag="VYN80_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1541718.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            5026..>5300
                     /locus_tag="VYN80_001856"
     CDS             5026..>5300
                     /locus_tag="VYN80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541719.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFES"
BASE COUNT         1751 a          803 c         1133 g         1613 t
ORIGIN      
        1 agtagaagat gctagagata gaaggggaat gcgactaaca aagtcccagg ttattgaatt
       61 actcctaaat accgttgatt atttcgatga tattatgggg gctatttact caaaaaaata
      121 agagcaaaat tgctattatt ttttacaaat tggtgtaaca ctagtgatat aggagtgatg
      181 aaatggttgt ttatattaga caaagtaaat tactaagtga ggtttcaatt aataaataca
      241 atgctcaagt aggtgcctac ttacaaggag aagaagttat tttatatcaa tctttttctg
      301 aaataaaaga gttaacaagt gaagatatag atgtagatta tataatggag actagagcat
      361 tactaaaaat gatgggctta aacgttccgg tttatgacta tcctattgag cttaaagagt
      421 tttatggtcg aaagatttat gctggtattt taggagagat tgtgaatata cctgataatt
      481 ggggaaaatt tattaagcct aaagctggtt caaaagtctt tactggaaga gttgttaatg
      541 gaacccatga tttaataggt attggtctac ctttcgacta tcctatatgg attagtgagg
      601 ttgtagaatt catagctgaa tggcgttgtt ttgtgttaga tggtcgcgta ttagatgttc
      661 gaccctatac aggtgattat catgcacaat ttgatgcaag tgtaattgat gaagcgatat
      721 catgttggaa agatgcgcca atagcttatg gactagatat cggtgttact cgcgatggca
      781 gaacacttgt tgttgaagta aatgatggtt atgcattggg aaattatggc ctatctcctt
      841 taaaatcaat caattttcat agagctaggt ggaaagaaat ggtaaaaccc tattttgaaa
      901 aaaatgaaat ttttaaaatc caacaggatg ttattttcta aaataaatat ggtagataac
      961 caatataaaa agcatcaaaa tgaacaagga ggaatctgat gcttggagca attattggag
     1021 atattgtcgg ttctgtttac gaatggaaca atatcaaaac aaaggacttt cctttatttc
     1081 ggaaggactg cttttttaca gatgacacgg ttatgacctg tgctgttgca gaagcaatta
     1141 tgaatggtgg acaaaaagac gacttcattg acgcgatgaa gaaatatggc agaatgtatc
     1201 cgaatgctga ttacggtgct cggtttaatg cgtggctaaa cagcgataac cgtgagcctt
     1261 ataatagctt tgggaatgga tcagctatgc gtatttctcc atgtgcttgg atcatggact
     1321 gtggttttta tgcgaaaact ggtatatggc catcatctag aggacttacg agtctttctg
     1381 cagaggtaac tcataatcat ccagaaggtg tcaaaggcgc tatggctaca gctgatgcta
     1441 tctttctatg tcgtttttac tttggtggtt attgtagaga gtacgagcaa tcaattaacg
     1501 acaatcctac agagtgtaaa agacgaatta aggattatat agaaaaagaa tacgattaca
     1561 atctatctca aactttagat gaaatccgtc ctaactatcg ttttaacgaa acatgtcagg
     1621 aaactgtccc tcaagccatt atcgcctttc ttgaaagtag agacttcgaa gatgcgataa
     1681 gaaatgccat ctcacttggt ggcgacagtg atactctcgc agcaatcact tgtagcatag
     1741 cagaggcagc ttacggtatt cctgattgga tcaaggataa ggactattct tacttggatg
     1801 aacccttaaa ggatgtagtt aggcgatggg aaaatagaat aaaagcgtat taatctccag
     1861 tacgtcagat ttctcttttc tgtattttca ttgtataata ggattacatt tacttgcata
     1921 cgacgaaatg taaacatttc tgtttaaaat taaggataaa atcaatgaaa aaagcaatgg
     1981 taattatcaa ccctacttct ggtggcgaga aggctttgga ttacaaagaa aagctggaga
     2041 ataaagcaaa agaatacttt gaatatgttg aaaccaaaat taccgaaaaa gcgctggatg
     2101 caacacattt tgctgaagaa gcttctcgtg agcagtatga tgcagtggtt gtgtttggtg
     2161 gagatggaac tgtcaatgaa gtcatttcag gtattgatga gagagactac attcctaagt
     2221 tagggattat cccaggcggt acgggtaacc tcattacaaa acttttggaa atcaatcaag
     2281 acatcgatgg cgcaattgac gaactggatt ttgatttaac caataagatt gatatcggta
     2341 aagcaaatga caactatttt ggttatatct ttagtatcgg ttctctgcct gaggcgattc
     2401 acaatgttga aatagaggac aaaacaaaat tcggtatttt aacctatgct gtaaatacca
     2461 tgaagtctgt catgacagat caggtcttta acattaaggt tgagacagaa aatggaaatt
     2521 atgttggtga agctagccat gttttggtcc ttttgacaaa ttacttcgct gataagaaaa
     2581 tctttgaaga aaacaaggac ggctatgcca acattttgat tctgaaagat gcctctatat
     2641 tctccaaatt atccgtcatt cctgatttat taaaagggga tgttgtcgca aatgataata
     2701 tcgagtatat caaagcgcgt aatattaaaa tctcttcaga tagtgaattg gagtcagatg
     2761 ttgacggaga taaatcagat aacctacctg tagaaatcaa agtcctagct cagcgagtag
     2821 aagtattttc aaaaccgaaa gaggattagt atatagagaa agcctttttt aaggcttttt
     2881 gtatacttta aaagatagtt cctttaacaa cggacattcc ttgcaaatag tttacaaaaa
     2941 tagtatactg gattcattga gcttgaaaac gtttgcgtaa aatttgaatg aatactttag
     3001 gagacaaatt gatggaattg agtgctattt accataggcc tgagtcggag tatgcctatc
     3061 tttataagga taagaaactc catattcgaa ttcgaactaa gaaaggggac attgaaagca
     3121 tcaacttgca ctatggggac ccttttatct ttatggagga gttttatcag gatacaaaag
     3181 aaatggtcaa gataacttct ggtaccttat ttgaccattg gcaggttgaa gtgtcagttg
     3241 actttgcacg tatccagtat ctctttgagc tcagagatac agaaggtcaa aatattttgt
     3301 atggcgataa agggtgtgtg gaaaattctc tagaaaatct tcatgcaatt gggaatggat
     3361 ttaagttgcc ttatcttcat gagattgatg cctgcaaggt tcctgactgg gtttcaaata
     3421 cggtatggta tcagatattt cctgaaagat ttgccaatgg caatgctcta ttaaacccag
     3481 aagggacttt agactgggat tcatctgtca cacctaagag cgatgatttc tttggtggtg
     3541 atttacaggg gattattgat catatggatt acttgcaaga cttgggtatt actggactat
     3601 atctttgtcc catctttgaa tctacaagca atcacaagta caatacgaca gattactttg
     3661 aaattgaccg tcattttgga gacaaggaga cctttcggga actggtggat caagcgcatc
     3721 atcgtggcat gaaagtcatg ctggatgcgg tatttaatca tattggttcg caatctcttc
     3781 aatggaaaaa tgtcgtcaaa aatggtgaac agtctgctta taaggattgg ttccatattc
     3841 aacaattccc agtgacaact gaaaagctag ttaataagag agacttaccc tatcatgttt
     3901 ttggtttcga ggactatatg cctaagctaa atacagccaa tccagaggtc aagaattatc
     3961 ttttaaaggt tgcgacttat tggattgaag agtttaatat cgatgcttgg cgtttggatg
     4021 tggctaatga gattgaccat cagttctgga aggattttcg taaggcagtt ttagctaaaa
     4081 atcctgatct ttatatccta ggagaagtct ggcatacatc tcagccttgg ctaaatggag
     4141 atgagttcca tgccgtcatg aattatcctt tatctgatag tatcaaggac tatttcttac
     4201 gaggaattaa gaagacagac cagttcatcg atgaaatcaa tggagagtct atgtattaca
     4261 agcagcagat ttcagaggtc atgtttaatc tcttggattc acatgataca gagcgaatcc
     4321 tgtggacggc caatgaagat gttcaactgg ttaaatcagc cttagccttt ctctttttac
     4381 aaaaaggaac accgtgcatt tattacggaa ccgagctagc cttgactgga ggaccagatc
     4441 cagattgtcg tcgttgtatg ccttgggaac gtgtatcaag tgacaatgat atgctgaact
     4501 ttatgaagag gctgattaag atttggaaat acgcgtcagt aatcatttcg catggcaagt
     4561 atagccttca agaaatcaaa tctgatctag tagctctgga atggaaatac gaaggacgga
     4621 tcctcaaagc aatattcaac caatcaacag aagattatct tttagagaaa gaagcagtag
     4681 cactagcaag caattgccaa gaattggaga atcagcttgt catctctcca gatggatttg
     4741 tgattttcta aaaactagtt gatgaagatt atggtacatt tcatatctta tatagtataa
     4801 taaggctagt tactaaactt gtaaaggaga acttaaatga attgtagagg acatgaaaca
     4861 agacaaagaa ttgttagaga ttttgaagtt tagcctaaag cacatattaa gctgttagca
     4921 aatcaacaaa aacatagtga tgcaggagca actattgaag atgaatatta tgtatttatc
     4981 gctgagagta aaattgatgg caagaaggaa gttattcagt gttgcatggg tgcggcaagg
     5041 gattttttag aactaattaa tcacaaaggg ctacctcttt ttaatccgct tgtaggtgat
     5101 tctcatgtaa ataatagaca agaatatgac aatacaggga gtgaaaattt acaacctgaa
     5161 aagtggaatg aaactgcaaa gcagctttat aatgctataa tgtggttgat tattttatgg
     5221 aatgctaagc cggatacacc tttatttaat tttaaagacg aagtaattaa gtataaaaca
     5281 tatgagcctt ttgaaagcag
//
LOCUS       DAVLWA010000105         5150 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLWA010000105 DAVLWA010000000
VERSION     DAVLWA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.105"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..314
                     /locus_tag="VYN80_001857"
                     /pseudo
     CDS             1..314
                     /locus_tag="VYN80_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284991.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            387..833
                     /locus_tag="VYN80_001858"
     CDS             387..833
                     /locus_tag="VYN80_001858"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541720.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            1173..1427
                     /locus_tag="VYN80_001859"
     CDS             1173..1427
                     /locus_tag="VYN80_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541721.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            1538..2644
                     /locus_tag="VYN80_001860"
     CDS             1538..2644
                     /locus_tag="VYN80_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1541722.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            2602..3279
                     /locus_tag="VYN80_001861"
     CDS             2602..3279
                     /locus_tag="VYN80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1541723.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            3503..4372
                     /locus_tag="VYN80_001862"
     CDS             3503..4372
                     /locus_tag="VYN80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1541724.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
BASE COUNT         1747 a          929 c         1060 g         1414 t
ORIGIN      
        1 actgatttta aacagcgagc attagattac atcaaagagg ggcacagcca tgtcgaggca
       61 gccaagtttt ttggtgttgg cgtcagaact ctcttcacgt gggaaaagaa agacgtgaac
      121 aagaacacat agagaggaaa aagcgagtcg tcaaaaaccg aaagattcct ttagaggaat
      181 tgaaagcctt tgtagaggct catccagatg cttttttacg ggaaattgcg gcacattttg
      241 attgtgctgt tccttcagta tgggcagctt taaagcagat taaggtcact ttaaaaaaag
      301 atgaggagct ttaaggaaca agacccagaa aagtagcctt atttcttaag aattttaata
      361 gtttaaagca cctagcacct gtttatattg atgaaacagg aatcgaccgc tatctctatc
      421 gtccttatgc aggggctcct agaggggaga aagtctatga aaagattagc ggacgtcgtt
      481 ttgagcgaac ttcaattgtt gcaggacaag tagacggaga gtttatagct cccatgattt
      541 acaagaaaag catgacaagc gatttctttg tggagtggtt caaaacgcaa ctcctacctg
      601 ctttgaagac acctcatgtt attgtcatgg gcaatgctgg ttttcatccc aagaacattt
      661 tggatgaact ctgcatccaa gataaacact ttttcttacc tctaccacct tattcaccgg
      721 atttgaatcc tattgagcaa gcttgggcta tcttgaaaaa gaaagtgacg gatttattaa
      781 gggaagttcc aactattttt gaatgtttgg aatgcttttt taaaactaga tgactataac
      841 ggttctaaag gaacctatcg agtagtcatt aaaactaagg atactgctgg ttaagagaag
      901 acggtataca atcaaaccat tcaccgtgta gccgaaatcg ttcagaatga agacttgtat
      961 aagaaaggtt tgaatgttga acttgcgcac caacaaatta agggattttt tgaagcagag
     1021 tttaaaaatc gtattaatgg agttcttaat actaaaataa aaaatagtac attaaatcgt
     1081 gtaaataaaa aaactataca ccagagcaac aaaaactcca tgatcaattt gaagcagaag
     1141 caacggaaga tgctaaaaaa caaggcgata ttgtgttgaa tgttgaccag gatttcatga
     1201 gcatatctaa gtctaataaa agtggttcag actggaagaa aactttcaca gtgaggataa
     1261 ccaataggct agcaaatgac ttgaataatg tcttgaaaca ggctgataaa gatactccta
     1321 ataccccaac ttggctaaac tcagctgctt ctaaagctaa agatgatgac agagtatata
     1381 aactactgaa gactcttata ccaggagaaa attacctatc atgttaagga taatcagcta
     1441 gaagtagaaa cagataaata cacatatact gccgctagaa atggtagtaa ggaagttggt
     1501 attcaagagt cagatatagc agcaactcta agtgccgatg aatataattc taatcgccaa
     1561 acttttgaga gagaatacaa atacaaaagc aaatgccctt aataatggtt gggctagatc
     1621 tggttctgaa gagttcaaaa agttctccca ctttgtaggg gtagacaaag ggattgtgcg
     1681 aacgaatgta ctgactggta aaaaactatc tgataagatt aggaaagaag tgggctctgg
     1741 agatagcaaa ctaggaaaag gcggctattt ctctactggg gatgttctat taggaaaaga
     1801 tgttgtttct tataccgtac aagtattttc agagaataat gaaagagtag gagtaaacac
     1861 tcaaagtcac cgtgttcagt ataatctccc aattctagct gacttttcag tcatccaaga
     1921 tactgtggaa ccatcacgaa ccgttgttga aaaaatcatt ccaaaactaa atattcccga
     1981 agaagagaaa gggaaaataa ccgaagaaat caagaaaaag aaaaaaacct cagaattggc
     2041 agaactaatc tcagaaaatg tgaaagttcg ctatgttgat gaacaagggc gtttgctatc
     2101 attgaaaaat gatactggaa ttggagaaaa agaaagtgac ggaacctaca ttaccaataa
     2161 aaaacaactg attggtacca gctacaatgt cacagataaa aaactcagta gcatgactac
     2221 tactgacgga aaatattata cttttaaaga agcagataca aattctgcaa gtttaactgg
     2281 gaatattgta agcgaaggta gaacagtgac cttagtttat agagaaagcg aagcgccaac
     2341 cactgctaca gtaacagcca attactataa agaaggtagc caagagaagt tggcagagtc
     2401 tgttataaaa gctgatttag cgataggttc tgagtatacc acagaatcaa aaactattga
     2461 agggaaaaca acaactgagg acaaagaaga ccgagttatc acaaggaaaa caacatacac
     2521 cttggtagca actcctgcaa atgcgtacca gaagacggtg caacagttga ctattactac
     2581 cgtgagaatg ttgaggaaac agtggttccc aaaacagcaa cctctactga gacgaagact
     2641 ataacgcgta tcattcatta cgttgataaa gttacgaacc aaaatgtaaa agaagatgtt
     2701 gttcaacctg taaccttaag ccgtacaaaa attgagaaca aggtcacggg agttgtaacc
     2761 tacggtgaat ggacaacagg aaactgggac gaggttgtat ctggtaagat tgacaagtac
     2821 aaagatccag atattccaac agttgaatca caagaagtta cgtcagactc tagtgataaa
     2881 gaaataacgg taaggtatga ccgtttatca acaccagata aaccaactcc agaaaaacca
     2941 gaaattccaa gtcctcagga accaggtact ccaggtgaac cgactccaga aaaaccaatc
     3001 ccacaaccaa atccagagca tccaagtgtt ccgacaccaa acccagaact accaaatcaa
     3061 gagactccaa taccagagcc aactccagaa ccaggtactc caaaaactga aactccagtg
     3121 aatccagacc cagaagttcc gacttatgag acaggtaaga gagaggaatt gccaaacaca
     3181 ggtacagaag ctaatgctac cttggctagt gctggtatca tgaccttgtt agctggtcta
     3241 ggattaggat tcttcaagaa aaaagaagat gaaaaataat agattttaga atctaggaac
     3301 caggaaaagc tcacagatgt gggctttttt cctggttttg agaacgaggt ctttcgtgaa
     3361 gaataaaaac gcttacaagt ccgttgaact gggaaactat gaatcctatt tttttaaaaa
     3421 tatttccaga aatcagttgc taaatagggt aaaaattgat aaaataagga atatctaaaa
     3481 aattttaagg agaatctagc aaatggattt cacatgggca ctgaagtatg ccactgaatt
     3541 tttgggaact gccattttga tcattcttgg gaatggtgca gttgccaacg ttgaacttaa
     3601 aggtacgaaa ggtcaccaaa gtggctggat cgtcatcgct gttggttatg gtatgggggt
     3661 tatgatccca gccttgatgt ttggtaacgt atctgggaat cacatcaacc ctgctttcac
     3721 tctagggctt gcagttagcg gtcttttccc ttgggcacaa gtggtacctt atattatcgc
     3781 gcaagtcttg ggagctatct ttggccaagc cttagttgtg gcaacatacc gtccattcta
     3841 cttgaaaact gaaaacccaa ataacatctt gggaactttc tcaactattt caagtattga
     3901 ccatggtaca aaagaaagtc gctatgcagc aactgtcaat ggtttgatta atgagtttgt
     3961 tggttcattt gttttgttct ttgcagctct tggtttgact aaaaacttct ttggtgctga
     4021 agtgcttcaa ttcatgaaac aaaaggcaat agaagcagga caaacagttg atttttctga
     4081 cttggctatt aaagcacagg tggctccaca cactgcttca ggactttctg tggctcactt
     4141 ggcacttgga ttcctcgtta tggctttggt aacatcactt ggaggaccta caggacctgc
     4201 cttgaaccca gcccgtgact tgggaccacg tctccttcat gctttccttc caaaatcagt
     4261 tcttggtgag cataaaggcg attcaaaatg gtggtattct tgggtaccag tagtagcacc
     4321 tatcgtagca gcaattgcgg cagtagctgt attcaaattc ctttatctct aagaaatagc
     4381 tcctttaaca tttgagtgag caccatctat aagtaagaga ggatcagact ggttctctct
     4441 ttttgatttt ttagggaaat gaaagaaact ctaaacaaac tcctctccag cagtggttta
     4501 gaagtctcag tgggctattc cagcttcaat ggactatagt aggttgcagt tgaaataata
     4561 gacccttgtt tctaaaacat tgtgagaaat tggtttgaat tctccaatca aattgtgcag
     4621 ttttcattct actatatatt atcggagatg ggttccctat cttgtaagtc tgctttatag
     4681 tgggttgaag ttggaatagt cctcccttct ttctcaaaca ttgtgaggaa ttgatttacc
     4741 ttcctcaaca aaatgttcag tttctatttc attttgctat aaaataagcg attagggggg
     4801 ctattcttcg acctacattg actctgctga gtcctatgat tgttatcgtt ttatctgcaa
     4861 ttttatactc aatgaaaatc aaagggcaaa ctaagaagct agccgcaggt tgttcaaaac
     4921 acagttttga ggttgtatag tagattgaaa ctagaatagt acacatctac ttctaaaaca
     4981 ttgttagaaa tcgatttgac tgtcctgatc gatttgctct attcttgttt cattttacta
     5041 tataaaccag agactgttta cattttcagc aagtgagtgg atggataatg ctgaaaactc
     5101 cttgaaggat aagtctattt agtactttct attaattagt taaattttta
//
LOCUS       DAVLWA010000106         5072 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLWA010000106 DAVLWA010000000
VERSION     DAVLWA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.106"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(139..1701)
                     /gene="guaA"
                     /locus_tag="VYN80_001863"
     CDS             complement(139..1701)
                     /gene="guaA"
                     /locus_tag="VYN80_001863"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1541725.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     ICETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1843..2541
                     /locus_tag="VYN80_001864"
     CDS             1843..2541
                     /locus_tag="VYN80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1541726.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2584..3480)
                     /locus_tag="VYN80_001865"
     CDS             complement(2584..3480)
                     /locus_tag="VYN80_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1541727.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGILQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(3489..4424)
                     /locus_tag="VYN80_001866"
     CDS             complement(3489..4424)
                     /locus_tag="VYN80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1541728.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4421..>5072)
                     /locus_tag="VYN80_001867"
     CDS             complement(4421..>5072)
                     /locus_tag="VYN80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEV1541729.1"
                     /translation="IETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRTRKVEGRK
                     KLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHAEDDRASL
                     VEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASLDFIPAQGQDLTV
                     DNTVTWDLSMLKFLVNELDIASLRQKFESIEYFIPKSEKFFLGKDRNNVELWFEEV"
BASE COUNT         1574 a         1113 c          942 g         1443 t
ORIGIN      
        1 agtttgaaaa ctacggtcta attttttgtc caaatgtaaa tcacttggaa ctattattca
       61 atttattctt tttagtaaat gttcccgttt taatcctctg taaccgttga ttttacaaag
      121 cttttaattt ttttgcgatt actcccactc aactgttgca ggtggtttac ttgtaatatc
      181 gtagacgata cggttaacat gatccacttc atttacgata cgtactgaga ttttttgaag
      241 tacttcccat ggaattttgg caaaatcagc agtcatacca tcgatagaag tgatagcacg
      301 gattgcaatc gtgtagtcat acgtacgacc gtcacccata acaccgactg aacgaacgcc
      361 tgtgttaaca gtgaagtatt gccaaatatc gcggtcaagt ccagctttag cgatttcttc
      421 acgaagaata gcgtctgatt cacgaacggt ttcaagtttc tcttcagtga tttcacccat
      481 gacacggata gcaagtcctg gtcctgggaa tggttggcgc catacgatat ggtctggcat
      541 accaagctct gtaccaagag cacgaacttc atccttgtaa agagtattga gtggttcaat
      601 caattcaaac tgcatatctt ctggaagacc acccacgttg tggtgtgact tgatagtttg
      661 agctgtatcc gtaccagact cgataacatc tgtatataaa gtaccttgag caaggaattt
      721 cacatctttg agcttgcttg cttcgtcatc gaatacatag acaaactcgt taccaatgat
      781 tttacgtttt tgttcaggat cagaaacgcc agcaagtttg tcaaggaaac gtttagcagc
      841 gtctgctttg acgatattca aaccaaactt accaccgagc atgtccataa cttgatcagc
      901 ttcgccttta cgaagaagac cgtggtctac gaagatacag atcaattgat cgccaatcgc
      961 tttttggaga agaaccccaa cgacagatga gtcaacacca cctgatagac caagaaggac
     1021 acgtttatca ccgacggttt cacaaatttt tttgatctgc atgtcaatga aattatccat
     1081 tgaccagtca cctttagcct tacaaatgtt aagggcaaag ttacgaagga tatcatttcc
     1141 gtatacagaa tgacgaactt ctgggtggaa ttggataccg taaatgtgtt tatctgggtt
     1201 ttcgatggct gcgtatgggc agtcagctga tgtacctgta cgaacaaagt cagcaggaat
     1261 ctcagtaacc gcatcaccat ggctcatcaa aacagtctgt tcatcaggtg ttgattcaaa
     1321 aagcgctgat ggtgtgtgag ttagggttga ttgaccgtat tcacgatttc cagcatcacc
     1381 tgcaggaaca acttttcctc caagtttatg ggtcaataac tgcataccat aacagattcc
     1441 caaaattgga attccgagtt cgaagatttc tgggtcaata tcaaatgaac catcttcata
     1501 tacagaattt ggaccacctg atagaataat tcctacagga ttgacttcac gaacttcagc
     1561 agctgaaatt ttatggcttt ttagttctga aaaaacacca atctcacgga tacggcgtga
     1621 aatcagctgg ttgtactggc taccatagtc caatacgatg attttttcta catcttgcaa
     1681 atcagttgaa atgttgctca tctttttcct ttctcaaaag aaatatcttt tcccaatatt
     1741 ctatcacaaa taagggcgaa ttaccaacta aacaaggcga agtttgactt tttctagttt
     1801 attggggtac aatagagaaa agatagaaat gaagtgaaaa atatgctacc tgcttatata
     1861 aaaatccatg atcagataaa aaaggatatt gacgagtacc gttgggctat tggtgagagg
     1921 cttcccagtg aaagagattt agctgagcag tttgcggtca gtcgcatgac cctccgccaa
     1981 gccgtatctc tattagtcga agaaggcgtc ttagagcgcc gtgtaggaag cggcaccttt
     2041 gtttccagta ctcgagtaca agaaaagatg cgagggacaa ccagttttac tgaaattgtc
     2101 aaatcccaag gtaaagttcc ctctagccag ctcatttcct acaaaaaaac cattcccaat
     2161 gagcaagaag ttgccaagct aggaattttt ccaacggaca atattatccg aatggagcgg
     2221 gtccgctatg ccgaccaagt tcccctagtt tatgaagttg cttctattcc tgaaaaattc
     2281 attaaggact ttaaaaaaga agaaatcacc agtcatttct tccaaacctt gcaaaaacat
     2341 ggctatcgta tcggcaaatc tcaacagacc atctatgctc gccttgctaa agaaaagatt
     2401 gcccactatt tggaagttga aaaaggacat gctattcttg gattgaccca ggtttcctac
     2461 ctagaagatg gtactgcttt tgaatacgta aaaagtcaat atgtaggcga acgctttgaa
     2521 ttttatcttg aaaataatta gattaccacg caaaaactct ctgtcacgga caaagagtct
     2581 tttttatttt tctaagaata aatctttcaa agaaatcaaa gttacagcaa acaatatcat
     2641 tgccatacca ataaaatcaa tgggataaaa ttgttcattc attattaaga aggcaaagaa
     2701 aaccgccgat attggctcaa ttgaagccaa caagcttgac ttgaccggtc ctatcagact
     2761 ggctccttta aggaaagctg tataggcaaa gacagtcccg ataaggataa tgcctgcaaa
     2821 cgcaaggaga aaatcaagac tagtcgggat atcggcctgt agaatccctg taaaaggaag
     2881 ggcgaccaaa cctgctatga ccattcccac accaatgacc aagctgctcc cccacttttt
     2941 aatcaaggct atgggtaaaa tgatatacaa agcataagtc aaggcagaaa agagacccca
     3001 gaacagacca gcaggtgtca tggataactg gtccaactgc ccatgtgttg cgatcaggaa
     3061 ggttcctccg atggcgaata tgatggaaac tatctctccc agtgtcggtg ccaccctatc
     3121 cttgatacag ctataaatta aaattccgac aggacaaaca tactgaagca ccgtcgctgt
     3181 tcccgcattg gtctcctgaa tagcagacag ataggcgaat tggttgagaa aaagaccaat
     3241 cagagcaaaa ataagaagag acagcaaact ctttctatcc tttaaaaaga ccagtatttt
     3301 atcctttgca gtagcataag ccaagagcat gagaattcca ccagcgatta aaagacgcaa
     3361 gttggtcaag accagagccg aaattccgtg tgccattaag tattggccac tcgttcctga
     3421 caacccccaa gcaataccag ccacaactgt tagtaaagtc ccttttaaac tatttgacat
     3481 gtctctcctt actcttcttt gcgaatcaag ttcatcactt gattgcggtc taatatccat
     3541 tgagctctct cgtccttttc ataggtacga ttcgatagga aaatagtggc ttcttgcttc
     3601 tgacgattcc acatgataaa ggtacctgta tagcccgtat ggtctagcca atctccttcc
     3661 aaattccatg ctaaagaacg ttccttgtca tccaaaggag aaaaattttg atttaagtct
     3721 cttgcaaaat catctgctaa atagtgttct aaaaagattt gtaaatcctt tatagtcgaa
     3781 aataaaccag cactcccagc atgtctaccc aggagacgag ccttgggatc atgcactatg
     3841 cctgcctcta cacctctaac tgttggaaca gcaagctcaa ctggcccaaa cttggtttcc
     3901 gtcattcccc aaggtttcca gacttgatcc tttaaaatca catccaaatc ttgattaaaa
     3961 attctttcca aaataaagcc caacagcaaa aaatggacat ccgaataaag aaaggctggc
     4021 tgacttcgtc tgttgagatg aaacatcgct tcctttaatt caggggctgt taaaagatca
     4081 cgattaggaa taaaaggatc aaggtctgtt gcatgagtca agagctggcg aatagtgatg
     4141 tctggataat cactctcagg taaaaaatct attaccagtc tatcaatatc taattgacct
     4201 atttcccaca agaaggtaca aactgtgcca accccaacaa ccttgctgac actagctagg
     4261 tcataaacta gtcctgcctc agtctgcaag ccatgctctg ggtcactctg gcctaaatag
     4321 aactctgtcc attgattgtc cttaaaatac gcaaaagagg ctccgggata aatccctgcc
     4381 tcgatttgtt cttctatttt tttaataatc ttggtccact tcatacttct tcaaaccaca
     4441 attcaacatt atttctatct ttaccaagga agaatttttc agacttagga ataaaatatt
     4501 caatagactc aaacttctgg cgaagacttg ctatgtctaa ttcattgacc aagaacttga
     4561 gcatagataa gtcccaggta accgtattgt ccacagtcaa atcctgcccc tgagctggaa
     4621 taaaatcaag ggatgcccca atttcagatg attccaagaa actttcgata tcagttggga
     4681 gatgtaattc catagaaatc tcaaatttac ttaaagaaat tgatgccaaa tctgtttgaa
     4741 attcaggctt ttctcctact tctactagac ttgctctgtc atcttccgca tgaatcaaaa
     4801 tcaaatcatc ttctggtgag aaaatttcaa aagcgtagcc attttgacct ttatataatc
     4861 gatgaatcga atctgtttta gataagattc cttcaatttc taagggattt tccaccttga
     4921 caatcaatct agctagtttt tttcttccct ctaccttacg agtacgcata ctgggagctt
     4981 cttctaaaac cagcttttca agacccgttt ggtcacctag tgacagaaag gccgattctt
     5041 ctaacaaggc cttcattcca agggtttcaa ta
//
LOCUS       DAVLWA010000107         4898 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLWA010000107 DAVLWA010000000
VERSION     DAVLWA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.107"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            81..608
                     /locus_tag="VYN80_001868"
     CDS             81..608
                     /locus_tag="VYN80_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541730.1"
                     /translation="MIKYKELKSYIDEWRYYSDENNPRLDLEYCKTKIVEKAKEFDLP
                     CQIDEEQIKLGGLFNKEIEECLVISHPNHQKDYVKFCFRLKNQGSVQLLTIDTLGESK
                     QLKKYYISEDNKRFREAIRESDLSLGQKLGAQLSNLTVSGLRTLGKNQSKIDAELQYC
                     EFLTEVLTQFRSNND"
     gene            618..2156
                     /locus_tag="VYN80_001869"
     CDS             618..2156
                     /locus_tag="VYN80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV1541731.1"
                     /translation="MSKAIGIDLGTTYSAVSVLSETGQPQILLNQDGENLTPSVVFFQ
                     DFDGKDEPLVGIQAKNLAASRPEAVVQYVKRQMGNPNWKFDSPSDTVYTAEEISAIIL
                     KRLKEGAENALGDKVEDVVITVPAYFDDARRTATKHAGEIAGLNVLRVLNEPTAAALA
                     YGISAEKNETVLVYDLGGGTFDVTLMKIKDGEFDVIATDGDRNLGGFDFDNALSMIIA
                     EKMEEQGAEDIYTDEHFTALLREKSENTKRGLTTVEKTNVFLDYKGKSYKIPITRVEF
                     EEATKSLMNRTEELLDDVVEESGMSWDEIDQVLLIGGSTRMPMVQRKLEEKIGKKIVY
                     SINPDEAVAQGAAIQAALEIANKSESADVSETVRGLAEKLVVSDVTSQALGTLALSNG
                     VKRNTIIIPKNSKVPNKYSEYFSTVVDNQQNVLVEVTQGDDEDPQFVTVIGESTIKLP
                     GDWPAGTVLKVTYHYDVDQTVFVEVHDSDNHLLGTFEVERQANLDKSAVEVASRKIKD
                     LTID"
     gene            2169..3536
                     /locus_tag="VYN80_001870"
     CDS             2169..3536
                     /locus_tag="VYN80_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1541732.1"
                     /translation="MSVINYYEELGISETSSLDDVKKSIKSNRRRYRQLTGSPNIDQR
                     SMAERKMEVIAQAEKVFESEETRQKYDRELENSKQSSEGVPDSTPTNHSNSSYLDSAR
                     QAFYSGKKSLAYSYIEEALKINRNDADVWYFKAMISLEDRKLSDAELAISEANRLRPK
                     NADILSLLGDVYCEQNQQKFAIQYYQEAFELSNNSFYLLKKGRSLFLFDQYKQAVKDV
                     RYLYDHYLEENKNNEEYIYYLLSCSYREIGDNEEAYNFARKLVDVSDSLEHKLHYAGI
                     LFLKSEDECENYLDTLNERYGNNKDFQQVYFKLLLDITIERMKKAPDTDVEAFFRKYI
                     DRTKYENEAVLDNKARVRIAEAYLAKAQSLGIENKIIDNDFYNEFIENKNYINYATTR
                     HSQGMGCLMFILVFAANWFLSGFFDKFVHSFWISNALSLALLSNIVLYYNYPKGWQIN
                     RRKKG"
     gene            3544..4002
                     /gene="grpE"
                     /locus_tag="VYN80_001871"
     CDS             3544..4002
                     /gene="grpE"
                     /locus_tag="VYN80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1541733.1"
                     /translation="MSEEVLHEKVSYIVDKVEDIESLITRRLLEDKAKNSLIEELKQY
                     LIYRQDLDKGEKFAPFMKQILQVIDRIESSEEKSDLLTSIAEELLQILSLNGLQVIDN
                     SGMIDPSMHEVVNTVAVTDEQSENNIVEVLQKGYLLNNRVLRPSKVTIAK"
     gene            4027..4839
                     /locus_tag="VYN80_001872"
     CDS             4027..4839
                     /locus_tag="VYN80_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HEV1541734.1"
                     /translation="MLRFFKHRRNILYFILGFLWGRRQNAKVSPEPPTPSTPKHSELP
                     SISKATHNGKMTGFEPQKLKTYQLYQHELMFGEPGKGLNKSGFDESAVNLGQEGEINF
                     AKALQKKGLLEKLVTFWSVHNLNLEDERVDADIDCVIVSGSTIWLVDLKFYASGNVIY
                     READGLLYTIDSATGAQIGRPKKMSPNMSYAEESFSHKFASLLKYYRLETRVVLMPTY
                     KGAGRLDNVFWPGHIKAVSLEEMLDELSREDKFRDTIGGQMIRQTFNLLLKR"
BASE COUNT         1778 a          631 c         1010 g         1479 t
ORIGIN      
        1 ataaatacgc ttttcgtaaa tcgagcgaag gctatgctat actgtaaccg taaacaataa
       61 gatataaaag gagattgttg atgattaaat acaaagaatt gaaaagttat atagatgagt
      121 ggagatatta ttccgatgag aacaatccaa gattggattt agaatattgc aagacgaaaa
      181 ttgttgagaa agcaaaagag tttgatcttc cttgtcaaat tgacgaagaa caaataaaac
      241 tcggcggttt gttcaataaa gagattgaag aatgtctagt tatttcgcac ccaaatcatc
      301 agaaagacta tgtaaagttt tgttttcgtt taaaaaatca aggatcagta caacttctga
      361 ctatagatac tttaggtgag agtaaacaat taaaaaaata ctatatatca gaagataata
      421 aaagattcag agaagcaatt agagaatcgg acttgtcatt gggtcagaaa ttaggagctc
      481 aacttagtaa cttaacagtt agcggtctta gaactttggg gaaaaatcag agcaagattg
      541 atgcagaatt acaatactgt gagtttttaa cagaggtact aacacagttt agatcaaata
      601 atgattagga gtgaaatatg agcaaagcaa ttggaattga tttaggaacg acttattcag
      661 cagtatctgt attatcagag actggacaac cacaaatttt attgaatcaa gatggtgaaa
      721 atttaactcc atctgttgta ttctttcagg attttgatgg gaaagatgaa ccacttgttg
      781 gtattcaagc taaaaatttg gcagcaagta gacctgaagc agttgtacag tatgtcaaaa
      841 gacaaatggg aaatccaaat tggaagtttg attctcctag tgatactgtt tatacagcag
      901 aggaaatttc agcaatcatt ttaaaaagat taaaagaagg agcagaaaat gcccttggtg
      961 acaaagtaga ggatgttgta ataactgttc cagcttattt tgatgatgcc cgtagaacag
     1021 caacgaaaca tgctggagaa attgctggac ttaatgtttt acgagtttta aatgaaccga
     1081 cagccgctgc acttgcatac ggtatatcag cagaaaaaaa tgagacagta ttggtatatg
     1141 atttaggtgg tggaactttt gacgtcacat taatgaaaat aaaagatggc gagtttgatg
     1201 ttattgccac cgatggagat cggaatctag gagggtttga ttttgataac gccttatcaa
     1261 tgattattgc tgaaaagatg gaagaacagg gagcagaaga tatttatacg gatgaacatt
     1321 ttactgcctt acttcgtgaa aaatcagaga atacaaagag aggactaaca actgtagaaa
     1381 aaacaaatgt ctttctcgac tataaaggaa aaagttataa aattccgata acacgtgtag
     1441 aatttgaaga agcaacaaaa agtctaatga atcgtacaga ggagctgtta gatgatgttg
     1501 ttgaagagtc tggaatgtca tgggatgaaa ttgaccaagt actattaata ggtggttcaa
     1561 ctcgaatgcc aatggtacaa agaaaattgg aagagaaaat tgggaagaag attgtatatt
     1621 ctattaatcc agatgaagct gtagcacaag gagcagctat tcaagcagca ttggaaatcg
     1681 ctaataaatc agaaagtgca gatgttagtg aaacagttcg tggattagca gaaaagttag
     1741 ttgtttcgga tgtcacttct caagccttgg gaactctggc tctttcgaat ggtgtgaaaa
     1801 gaaatacaat tattattcct aaaaattcca aggtaccaaa caagtactct gagtattttt
     1861 caacagttgt agataatcaa caaaatgtgt tagtagaggt tactcaaggg gatgacgagg
     1921 atccgcaatt tgttacggtt ataggagaga gtaccattaa attaccaggt gactggcctg
     1981 caggaacggt tttgaaggta acttatcatt atgatgtcga tcaaacagtt tttgtagagg
     2041 tacatgattc ggataatcac ttattaggga cttttgaagt agagagacaa gcaaatctcg
     2101 ataaaagtgc tgtagaagta gctagtcgta aaataaaaga tttaactatt gattagggga
     2161 ggaacacaat gtcggtcata aactattatg aagagttagg gatttcagaa acaagttcat
     2221 tagatgatgt taagaaaagt atcaagtcaa atagaagacg ctatcgccag ttgacaggtt
     2281 caccaaatat tgatcaaaga tcaatggctg agagaaagat ggaagtcatt gcacaggcag
     2341 aaaaagtatt tgaaagtgaa gaaaccagac aaaaatatga tagggaatta gaaaattcta
     2401 aacaaagttc tgaaggagtc cctgattcga ctccaactaa tcattcaaat tcaagctatc
     2461 ttgattcagc tagacaggcc ttttattcag gaaagaaatc attagcttat tcctacattg
     2521 aagaagcctt aaaaataaat cgaaatgatg cagatgtttg gtatttcaag gctatgatta
     2581 gccttgaaga tagaaagtta tcagatgcag aattagctat tagtgaagct aatagattga
     2641 gacctaaaaa tgcggatatc ttatctttgc taggtgatgt ttattgtgaa cagaatcagc
     2701 aaaagtttgc aattcagtat tatcaggaag cttttgaact atctaataat tccttttatc
     2761 ttttgaaaaa gggaagatca ctatttttat ttgatcagta taaacaggca gtaaaagatg
     2821 tgcgatatct gtatgatcat tatttagaag aaaataaaaa taatgaagaa tatatttatt
     2881 accttttatc atgctcttat agagaaattg gagataatga agaagcctat aattttgcaa
     2941 gaaaattagt agatgtttca gattcactag agcataaact acattatgca ggtattctat
     3001 ttttgaaatc tgaagatgag tgtgagaatt atttagatac cttaaacgaa cgctatggta
     3061 acaataaaga tttccagcaa gtgtatttta agttactatt agatatcacg atagagcgta
     3121 tgaaaaaagc acctgataca gacgttgagg ctttctttag aaaatatatt gatcgaacaa
     3181 aatatgagaa tgaagcagtt ctagataata aggcgcgtgt tcgcattgca gaagcttact
     3241 tggccaaggc acaaagttta gggattgaaa ataaaattat agataatgat ttttacaatg
     3301 aatttattga aaataaaaat tatatcaatt atgccacaac acgccattca caaggtatgg
     3361 gatgtctcat gttcatctta gtttttgcag ccaattggtt tttgagtggt ttctttgata
     3421 agtttgtaca tagcttttgg attagtaacg cattaagttt ggcattgtta tcaaatattg
     3481 tcttgtatta caattaccct aaaggatggc aaataaatag aagaaagaaa ggataaagtt
     3541 tttatgagtg aagaagtatt gcatgaaaaa gtttcgtata tcgtagataa agttgaagat
     3601 atcgaatctt taattacacg ccgtttattg gaagataaag ccaaaaatag tctgattgaa
     3661 gaattgaagc agtatttaat ctatagacag gatttagata agggtgagaa atttgctcca
     3721 tttatgaaac agattcttca agttattgat cgtattgaat catctgaaga aaaatcagat
     3781 ttgcttactt caattgctga agagcttctg caaattcttt cattgaatgg attacaagtt
     3841 attgataata gtggaatgat tgacccaagt atgcatgagg ttgttaatac agtagcagtt
     3901 actgatgaac aaagtgaaaa taatattgtt gaagtattgc aaaaaggata tttgttaaat
     3961 aacagggttc ttagaccaag taaagtgact attgctaaat aagatatttt ttaggaggtg
     4021 gagaaattgt tgcgcttttt taagcatcgc agaaacatac tatattttat attaggattt
     4081 ctttggggca gaagacaaaa tgctaaggtt tctccagaac cacctactcc ttccacacct
     4141 aaacatagtg aacttcccag tatttccaag gcaactcata atggtaaaat gacgggattt
     4201 gaaccacaaa aacttaagac ttatcaactt taccagcatg aattgatgtt tggagaacct
     4261 ggaaaaggat tgaataaatc aggattcgat gaatctgctg ttaatcttgg tcaagagggt
     4321 gaaatcaatt ttgctaaagc attacaaaaa aaagggcttt tagaaaaact agttaccttt
     4381 tggtctgttc ataatctcaa tttagaagat gaaagggtag atgcggatat tgattgtgtt
     4441 attgtttctg gatctacaat ttggttagta gatttaaaat tttatgcttc aggaaatgtg
     4501 atttatagag aagcggatgg tttattgtac actatagata gtgctacagg tgctcaaata
     4561 ggcagaccta aaaaaatgtc ccctaacatg tcttatgcag aagaaagctt ttcccataaa
     4621 tttgcgagtt tattaaaata ttatcgcttg gaaacacgtg ttgtcttaat gcctacctat
     4681 aagggtgcag gtagattgga taatgtcttt tggccaggtc atattaaggc tgttagtcta
     4741 gaagagatgt tagatgagtt atctagggaa gataagttta gggatacaat tggtgggcag
     4801 atgattcggc agacatttaa tttacttttg aaacgttaaa ttatgtaaaa atagtaaaga
     4861 attgatgttg tagatttttt atatgagtta tattttaa
//
LOCUS       DAVLWA010000108         4895 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLWA010000108 DAVLWA010000000
VERSION     DAVLWA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.108"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            28..1573
                     /locus_tag="VYN80_001873"
     rRNA            28..1573
                     /locus_tag="VYN80_001873"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1625..1697
                     /locus_tag="VYN80_001874"
     tRNA            1625..1697
                     /locus_tag="VYN80_001874"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1658..1660,aa:Ala,seq:tgc)
     gene            1822..4723
                     /locus_tag="VYN80_001875"
     rRNA            1822..4723
                     /locus_tag="VYN80_001875"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4800..>4895
                     /gene="rrf"
                     /locus_tag="VYN80_001876"
     rRNA            4800..>4895
                     /gene="rrf"
                     /locus_tag="VYN80_001876"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1359 a         1002 c         1452 g         1082 t
ORIGIN      
        1 tgacagaaat gagtgagaac tcaaactttt aatgagagtt tgatcctggc tcaggacgaa
       61 cgctggcggc gtgcctaata catgcaagta gaacgctgaa ggaggagctt gcttctctgg
      121 atgagttgcg aacgggtgag taacgcgtag gtaacctgcc tggtagcggg ggataactat
      181 tggaaacgat agctaatacc gcataagagt ggatgttgca tgacatttgc ttaaaaggtg
      241 cacttgcatc actaccagat ggacctgcgt tgtattagct agttggtggg gtaagggctc
      301 accaaggcga cgatacatag ccgacctgag agggtgatcg gccacactgg gactgagaca
      361 cggcccagac tcctacggga ggcagcagta gggaatcttc ggcaatggac ggaagtctga
      421 ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa
      481 gaacgagtgt gagagtggaa agttcacact gtgacggtat cttaccagaa agggacggct
      541 aactacgtgc cagcagccgc ggtaatacgt aggtcccgag cgttgtccgg atttattggg
      601 cgtaaagcga gcgcaggcgg ttagataagt ctgaagttaa aggctgtggc ttaaccatag
      661 taggctttgg aaactgttta acttgagtgc aagaggggag agtggaattc catgtgtagc
      721 ggtgaaatgc gtagatatat ggaggaacac cggtggcgaa agcggctctc tggcttgtaa
      781 ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      841 ctgtaaacga tgagtgctag gtgttagacc ctttccgggg tttagtgccg tagctaacgc
      901 attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg
      961 gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg
     1021 tcttgacatc cctctgaccg ctctagagat agagttttcc ttcgggacag aggtgacagg
     1081 tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     1141 caacccctat tgttagttgc catcatttag ttgggcactc tagcgagact gccggtaata
     1201 aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca
     1261 cgtgctacaa tggctggtac aacgagtcgc aagccggtga cggcaagcta atctcttaaa
     1321 gccagtctca gttcggattg taggctgcaa ctcgcctaca tgaagtcgga atcgctagta
     1381 atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
     1441 accacgagag tttgtaacac ccgaagtcgg tgaggtaacc gtaaggagcc agccgcctaa
     1501 ggtgggatag atgattgggg tgaagtcgta acaaggtagc cgtatcggaa ggtgcggctg
     1561 gatcacctcc tttctaagga taaggaactg cgcattggtc ttgtttagtc ttgagaggtc
     1621 ttgtggggcc ttagctcagc tgggagagcg cctgctttgc acgcaggagg tcagcggttc
     1681 gatcccgcta ggctccattg gtgagagatc accaagtaat gcacattgaa aattgaatat
     1741 ctatatcaaa tagtaacaag aaaataaacc gaaaacgctg tagtattaat aagagtttat
     1801 gactgaaagg tcagaaaata aggttaagtt aataagggcg cacggtggat gccttggcac
     1861 taggagccga cgaaggacgt gacaaacgac gatatgcctt gggtagctgt aagtaagcga
     1921 tgatccaggg atttccgaat gggggaaccc aacaggtaat acctgttacc cacatctgtt
     1981 aaggatgtga ggaggaagac gcagtgaact gaaacatcta agtagctgca ggaagagaaa
     2041 gcaaaagcga ttgccttagt agcggcgagc gaaacggcag aagggcaaac cgaagagttt
     2101 actcttcggg gttgtaggac tgcaatgtgg actcaaagat tatagaagaa tgatttggga
     2161 agatcagcca aagagagtaa tagcctcgta tttaaaatag tctttgtacc tagcagtatc
     2221 ctgagtacgg cgggacacgt gaaatcccgt cggaatctgg gaggaccatc tcccaaccct
     2281 aaatactccc tagtgaccga tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc
     2341 gggaggggag tgaaatagaa cctgaaaccg tgtgcctaca acaagttcga gcccgttaat
     2401 gggtgagagc gtgccttttg tagaatgaac cggcgagtta cgttatgatg cgaggttaag
     2461 ttgaagagac ggagccgtag ggaaaccgag tctgaatagg gcgccttagt atcatgacgt
     2521 agacccgaaa ccatgtgacc tacccatgag caggttgaag gtgcggtaag acgcactgga
     2581 ggaccgaacc agggcacgtt gaaaagtgct tggatgactt gtgggtagcg gagaaattcc
     2641 aaacgaactt ggagatagct ggttctctcc gaaatagctt tagggctagc gtcgacatta
     2701 gagattcttg gaggtagagc actgtttggg tgaggggtcc atcccggatt accaatctca
     2761 gataaactcc gaatgccaat gaattatggt cggcagtcag actgcgagtg ctaagatccg
     2821 tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataatt gttaagtgga
     2881 aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct attcattcaa
     2941 agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc ggggctaaaa
     3001 caatttaccg aagctgtgga tacctttata ggtatggtag gagagcgttc tatgtgtgat
     3061 gaaggtatac cgtgaggagt gctggaacgc atagaagtga gaatgccggt atgagtagcg
     3121 aaagacaggt gagaatcctg tccaccgtaa gactaaggtt tccaggggaa ggctcgtccg
     3181 ccctgggtta gtcgggacct aaggagagac cgaaaggtgt atccgatgga caacaggttg
     3241 atattcctgt actagagtat gtagtgatgg agggacgcag taggctaact aaagcagacg
     3301 attggaagag tctgtctaag cagtgaggtg tgaattgagt caaatgctta attctataac
     3361 attgagctgt gatggggagc gaagtttagt agcgaagtta gtgacgtcac actgccaaga
     3421 aaagcttcta gcgtttaaac atactctacc cgtaccgcaa accgacacag gtagtcgagg
     3481 cgagtagcct caggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
     3541 acttcgggag aaggggtgct gacttaaagt cagccgcagt gaataggccc aagcaactgt
     3601 ttatcaaaaa cacagctctc tgctaaatcg taagatgatg tatagggggt gacgcctgcc
     3661 cggtgctgga aggttaagag gagtgcttag cgtaagcgaa ggtatgaatt gaagccccag
     3721 taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc
     3781 cgacccgcac gaaaggcgta atgatttggg cactgtctca acgagagact cggtgaaatt
     3841 ttagtacctg tgaagatgca ggttacccgc gacaggacgg aaagacccca tggagcttta
     3901 ctgcagtttg atattgagtg tctgtaccac atgtacagga taggtaggag tctaagagat
     3961 cgggacgcca gtttcgaagg agacgctgtt gggatactac ccttgtgtta tggccactct
     4021 aacccagata ggtgatccct atcggagaca gtgtctgacg ggcagtttga ctggggcggt
     4081 cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc agaatggttg gaaatcattc
     4141 gcagagtgta aaggtataag ggagcttgac tgcgagagct acaactcgag cagggacgaa
     4201 agtcgggctt agtgatccgg tggttccgta tggaagggcc atcgctcaac ggataaaagc
     4261 taccctgggg ataacaggct tatctccccc aagagttcac atcgacgggg aggtttggca
     4321 cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt cccaagggtt gggctgttcg
     4381 cccattaaag cggcacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
     4441 gtcgcgggcg taggaaattt gagaggatct gctcctagta cgagaggacc agagtggact
     4501 taccgctggt gtaccagttg tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg
     4561 ataaacgctg aaagcatcta agtgtgaaac ccacctcaag atgagatttc ccatgattat
     4621 atatcagtaa gagccctgag agatgatcag gtagataggt tagaagtgga agtgtggcga
     4681 cacatgtagc ggactaatac taatagctcg aggacttatc caaagtaact gagaatatga
     4741 aagcgaacgg ttttcttaag ttgaatagat attcaatttt gagtaggtat tactcagagt
     4801 taagtgacga tagcctagga gatacacctg tacccatgcc gaacacagaa gttaagccct
     4861 agaacgccgg aagtagttgg gggttgcccc ctgtg
//
LOCUS       DAVLWA010000109         4855 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLWA010000109 DAVLWA010000000
VERSION     DAVLWA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.109"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            80..676
                     /locus_tag="VYN80_001877"
                     /pseudo
     CDS             80..676
                     /locus_tag="VYN80_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            752..1201
                     /locus_tag="VYN80_001878"
     CDS             752..1201
                     /locus_tag="VYN80_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1541735.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1214..2289
                     /locus_tag="VYN80_001879"
                     /pseudo
     CDS             1214..2289
                     /locus_tag="VYN80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2279..3924
                     /locus_tag="VYN80_001880"
                     /pseudo
     CDS             2279..3924
                     /locus_tag="VYN80_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4027..4719)
                     /locus_tag="VYN80_001881"
     CDS             complement(4027..4719)
                     /locus_tag="VYN80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1541736.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1531 a         1032 c          837 g         1455 t
ORIGIN      
        1 tgttatagat tcaatatact atagaataga attgtaggtt ttaaaataaa cttaaataac
       61 acaacggaga tatcccatca tgaaaaagaa atggatgtat tatgctgctt gttcttctaa
      121 tgaatctgcc gatgacagtt catctgataa aggagacggt ggttcgctag tcgtttattg
      181 accaaactca gaggacttaa ttggagcaac tattcctgcc tttgaagaaa aatatggtat
      241 caaagtagaa ctgattcaag ctggtactgg agaacttttc aaaaaactag agtcagaaaa
      301 agaagttcct gtagctgatg ttatctttgg tggttcttat acacaatatg ctacccacgg
      361 agaactcttt gaaaactata cttcaaaaga aaatgataat gttatcaaag aatatcaaaa
      421 cacaactggc tactctactc cttatacact agatggtagt gttttaatcg tcaaccctga
      481 tttaactaaa ggcatgaaca tcgaaggata taacgatctt ctcaaacctg aactaaaagg
      541 aaaaatcgca actgccgacc cagcaaactc ttctagcgcc tttgctcaat taacaaatat
      601 gctacaagct caaggtggtt acaaagatga taaggcttgg tcttatgtaa aagatctttt
      661 cacacttatt gatggtatag taaaatgaaa taagaacagg acaaatcaat caggacagtc
      721 aaatcgattt ctaacaatat tttagaagta gatgtgtact attctagttt caatatacta
      781 taaaatcggt ttaagttcat ctggtgtcta taaagcagtc gctgatggag aaatgactgt
      841 tggtctctct tatgaagatc cagcagttaa actcttaaat gacggagcta acattaaggt
      901 agtctatcca aaagaaggaa ccgtcttcct acctgctagt gctgctatcg ttaaaaaatc
      961 taaaaatatg gaaaatgcca agaaatttat cgattttatt atctctcaag aagtacaaga
     1021 tacacttggt acaaccacta ctaaccgtcc tgttcgtaaa aatgctaaaa caagcgaaaa
     1081 catgaaacca attgacaaaa tcaaaacact cactgaagat tatgattatg tcatcaagaa
     1141 taaatcagat atcgttaaga aatacaacga agtctttaca gatatccaat ctaaacagta
     1201 aaagaggttc actatgagtg agatcaaaat tattaacgcc aaaaaaatct accacgatgt
     1261 ccctgttatt gagaatttga acattacaat tccaaaattc ttggagcttc aggatgtggg
     1321 aaaacgaccc ttcttcgtat gattgcaggt ttcaacagta tcaaagatgg agaattttac
     1381 ttcgatgata caaaaatcaa taatatggaa cccagcaaac gcaatatcgg gatggttttc
     1441 caaaactacg ctattttccc acatttgact gtccgagaca acgttgcttt tggtcttatg
     1501 caaaagaagg ttccaaaaga agaattgatt caacagacca acaagtatct tgaactcata
     1561 caaattgctc aatatgcgga tcgaaagccc gataaactca gtggtggaca acaacaacgt
     1621 gtcaccttgg catgcgcctt agcggttaat ccaagtgttc tcctcatgga cgagccactt
     1681 agtaatctgg aggccaaact tcgcttggat atacgtcaag ccatccgaga aatccaacac
     1741 gaagtgggaa ttacaactgt ttatgtaacc cacgaccaag aagaagccat ggctatttca
     1801 gaccaaattg ctgttatgaa agatggggtg atccaacaaa tcggccgacc aaaagaactc
     1861 tatcataaac cagctaatga gtttgtggca acctttatcg gacgcacaaa tattatccct
     1921 gccaatcttg aaaaacggag cgacggcgct tatatcgtct tttcagatgg ctatgctctt
     1981 cgaatgctag ctcttgatca ggttgaggag caagctattc atgtaagcat tcgtcccgaa
     2041 gagtttatca aagatgaatc tggagatatt gaaggaacta ttagcgatag cgtctatctt
     2101 ggactaaata cggagtattt cattgagaca ggttttgcct caaaaattca agttagtgaa
     2161 gaatcaactt ttgaagaaga tctacaaaaa ggcaatcgta ttcgtctacg aatcaatacg
     2221 caaaaattaa acagcttttc tgcagatggt tcccaaaatc tgataaaagg agtcaaccat
     2281 ggaatgtaaa aaactaaata tttggacagc ttcctctttc ttcctctttc ttacctatcc
     2341 tatctttctc gtttatccta tcgttaccgt gctcaagcaa gcacttatac atgaaggaca
     2401 attctcacta gctaattttg tcactttctt tagtaaagcc tactactctg agacactagt
     2461 caacagtttc aaggtttcca ttaccgctac tatcacttcc ttagttgtag gaaccctatt
     2521 agcttatctc ttctctatgt atgacttcaa ggggaagaaa tttctacaaa tattgattat
     2581 cattgcttcc atgtcagctc ctttcgtagg agcctactcc tgaattctct tgctgggacg
     2641 aaatgaggtc atcactaaat ttttgacaaa tgcccttcat cttccagcta tcgatattta
     2701 tggattcaaa ggaattgtac ttgtctttac actgcaacta ttcccactgg tatttctata
     2761 tgttgctgga acaatgaaca gtattgacaa ttctctactt gaagcagctg aaagcatggg
     2821 gtccttcgga tttaagcgta tcgtaacggt tgttttacct ctcctagttc caaccttact
     2881 agcagatcct tgcttgtatt tatgagagca ttctcagact ttggaacgcc tatgttgatt
     2941 ggcgaaggat atcggacttt ccctgtcctg atttataccc aatttattag cgaggttgga
     3001 ggaaattctg cttttgcaat tatggcgatt atcattgcct tggcaatttt ccttatccaa
     3061 aaacacattg caaaccgcta cagtttcagc atgaatctgc tccatccaat tgagcctaaa
     3121 aaaactacaa aaggaaaaat agctgccatt tatgcaacag tctacggaat tatctttatc
     3181 tctgttttac ctcaaatcta cttaatttat acctctttcc taaaaacatc aggtatggta
     3241 tctgttaaag gttattctcc aaacagttac aaggtagctt tcaatcgtat gggatctgct
     3301 attttcaata ccattcgtat ccctttgatt gccttagttc tagttgttct atttgcgaca
     3361 tttatctcct acctagccgt tagaaaacgg aatttgttta caaacttaat tgacagcctc
     3421 agtatggtac cttatattgt accaggaacc gttctaggga ttgccttcat ttcttccttc
     3481 aatactggtc tatttggaag tggatttctt atgattacag ggactgcttt catcttgatt
     3541 atgtctctat ctgtcagaag attaccgtat actattcgct catctgttgc tagcttacaa
     3601 caaatagcac caagtattga agaagctgct gaaagcttag gaagtagtcg tctcaatacc
     3661 tttgctaaga ttacaactcc aatgatgcta tctggtatca tttctggagc catcttatct
     3721 tgggtcacaa tgatttcaaa actctctact tctatcctcc tctacaatgt caaaacaaga
     3781 acaatgactg tagctattta tacagaggtt ctcagaggaa attacggtgt agccgcagcc
     3841 ttgtcaacta tcctgactgt tctaacagta ggttccttgc tcttgtttat gaaaatctct
     3901 aaaagcaata gcattacact ttagttttcc acatcaaaaa cagccgaaag gagtaccctc
     3961 ggctgttttt tcttatcttg atattcttat caaatcccta gctcatggca agcaagtcta
     4021 gactgattaa atagagcctt cttccatctt ctcacgtcct agctctcggt aactacggtc
     4081 accgacaact tcaaccctaa actgaccgtc ctcatattca agaatcgtca cgctaccatt
     4141 atctagacca tgcggatgca tgccattaat cagataaaca atggttccaa tagtcattcc
     4201 atggctgaca acaagggcgt tacctccacc ttgatcttcc atttcttttg caatcatttc
     4261 aaagccttcc ttgattcggc cactgagttt ttcccagcct tcagcccaac cagctgtatc
     4321 gacctctacc aagccctcag ccagttcagc ataagacaat tggtgaacgt ggtccacatt
     4381 aaagatacga ggaataatgc ccatgaaaag atcgccatca taggctccat caaaactacc
     4441 gaaacaccat tctctgatac gcttgtccat gcgataaggg attttcccct gcaagccaag
     4501 ttcttcaagg ataattccca tggtctgaat ggtacgacca gaatcactcg aataagcgcg
     4561 ctcaaactgt agatcagatt ctcgcaaacc gattcctaac tcttgaatcc ctcgttcacc
     4621 ttcagcagtt aagggagtat cgctccaacc ttgcgcgcga ccaatcgtgt taaacatggt
     4681 cttgccatga cgtaccaaat acaatcgtac ttttaccatt ttccgtcctc cgtttgcttc
     4741 tattatatca tgaatgataa aataaaagcc acccatacag gcgacttttg aaggagatta
     4801 ttatgaaaaa gtttaggagt tcattaaata aagatattag atgaaaatca aattc
//
LOCUS       DAVLWA010000110         4795 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLWA010000110 DAVLWA010000000
VERSION     DAVLWA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.110"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            99..1622
                     /locus_tag="VYN80_001882"
     CDS             99..1622
                     /locus_tag="VYN80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541737.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1606..3327
                     /locus_tag="VYN80_001883"
     CDS             1606..3327
                     /locus_tag="VYN80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541738.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3338..3922
                     /locus_tag="VYN80_001884"
     CDS             3338..3922
                     /locus_tag="VYN80_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1541739.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            3922..4617
                     /locus_tag="VYN80_001885"
     CDS             3922..4617
                     /locus_tag="VYN80_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1541740.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4605..>4795
                     /locus_tag="VYN80_001886"
     CDS             4605..>4795
                     /locus_tag="VYN80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541741.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEI"
BASE COUNT         1710 a          640 c          911 g         1534 t
ORIGIN      
        1 agttaataat taattcaaac ttatccggtg cagagagtat agcattaaaa tctgttatta
       61 cactaacaag tggagcgata ttttattttg tctcaggaat gttttcacat atcttgggat
      121 ttaggcttga aacaaattta agaaaaaggg gaatcgatgg tctggaaaaa gcaagtttta
      181 ggttctttga cttaaatcca tctggtcaaa taagaaagat catagatgat aatgcagcac
      241 aaactcatca ggtggtagca cacatgattc ccgatagttc tcaggcaata atcacacccg
      301 tacttgtact tgcacttggc tttatagtaa gtataagggt tggcataatt ttgcttgctc
      361 ttactataat tggtggctta attttagggg caatgatggg cgagcaagaa tttatgaaga
      421 tataccaaga atccctatct aaactaagtg ctgaaactgt tgagtacgtg agaggaatgc
      481 aagttgtaaa aatatttaaa gcaaatgtag agtcttttaa aagcttttat aaggcgataa
      541 aagattactc aaagtatgct tatgattatt ccctatcttg taaaaggcct tatgttttgt
      601 atcaatggtt attttttgga ctgattgcaa ttttaattat tcctatagtt tattttatga
      661 ctagcttagc tagcgcaaag gtgattttac ttgagcttat catgatttta tttttatcag
      721 gagttctctt tgtttcattc atgagaatga tgtggtactc catgtatatt tctcaaggaa
      781 attatgcagt agatacttta gaggcgcttt acgaagatat gcaaaaagac aaattagtgc
      841 atggtaatgt caataatttt aaaaactata atatagaatt tgagaatgtt agctttgctt
      901 ataatgataa agctgtcatt gaaaatttat cctttaattt agaagaagga aagtcctacg
      961 cacttgtcgg ttcatctgga tcaggcaaat caacagtagc aaaacttata tcaggttttt
     1021 acaatgttaa taaaggaagc ataaagatag gcgggatagc aataagtgaa tattctgacg
     1081 aagccttaat taaagccatt tcctttgttt ttcaagattc aaaattattc aagaagagca
     1141 tttatgataa tgtagcttta gctaataaag atgcgacgaa agatgacgtt atgagagcct
     1201 taaaattagc aggatgcgat ttaatattag acaaattccc agaaagagaa aatacaatca
     1261 taggctcaaa aggtgtttat ttatccggtg gagaaaaaca aagaattgca attgctagag
     1321 caattttaaa ggattccaaa attattatta tggatgaagc atcagcatct attgacccag
     1381 ataacgagtt tgaattgcaa aaagctttta aaaatcttat gaaggataaa acagttatca
     1441 tgattgcaca caggctatct acaattaaag accttgatga aattattgtc atggatagtg
     1501 gaaaaattat agaaagaggg tctgacaaag aattaatgtc aaaagataca aggtataaga
     1561 gcctgcaaga gatgtttaac agtgcgaatg aatggagggt ttcaaatgaa agagttttat
     1621 aaaaaaagat ttgctcttac agatggagga gcaagaaatt taagtaaagc aacactggct
     1681 tcatttttcg tttattgtat aaacatgctt cctgccatat tacttatgat ttttgctcag
     1741 gaagttttgg aaaatatggg caaaagcaat ggcttttata tagtattctc agttttgact
     1801 ttgatagcaa tgtatatttt gctttctatc gaatacgata aattatataa cacaacctat
     1861 caagaaagtg cagatttaag aataaggaca gcggagaatt tatcaaaatt acctctatct
     1921 tacttttcta aacatgacat ttccgacatt tcacaaacaa tcatggctga tattgaaggc
     1981 atagagcatg caatgagcca ctcaatacca aaggtgggcg gcatggtact gtttttccca
     2041 ttaatatctg taatgatgct agcgggcaat gtcaagatgg gtttagctgt aattattcca
     2101 tctattttaa gctttatatt tataccttta tctaaaaaat atcaggttaa tggacagaat
     2161 agatattatg atgtcttaag aaaaaactca gaaagctttc aagaaaatat cgaaatgcaa
     2221 atggagatta aagcatataa tttatcgaag gatattaaag atgacttata taaaaaaatg
     2281 gaagatagtg agagagtaca cttaaaggca gaagtaacta caattttaac tttgtctata
     2341 tcttcaatat ttagctttat atctcttgct gttgtgatat ttgtcggcgt aaatctaatt
     2401 attaataaag agataaattc tctctacctt ataggatatt tactagctgc tatgaagata
     2461 aaagactctt tagatgcatc taaagagggc ttgatggaaa tattttattt atcgcccaaa
     2521 atagaaagat taaaagaaat tcaaaatcaa gatttacaag aaggcgatga ctatagctta
     2581 aaaaaatttg atattgatct aaaagatgtt gagtttgcct acaataaaga cgcaaaagtt
     2641 ttaaatggtg taagttttaa agctaagcag ggagaggtca ctgctttggt aggtgcaagt
     2701 ggctgcggta aaacaactat cttgaaactt atatcaagac tttatgatta tgacaaggga
     2761 caaatcttaa tcgatggcaa agatataaaa gaaatatcaa cagaatctct ttttgataag
     2821 gtctctatag ttttccaaga tgtggttctc tttaatcaaa gcattatgga aaatattaga
     2881 atcggaaagc aagatgcaag tgacgaagag gttaaaagag cagcaaaact tgcaaattgc
     2941 acagatttta tagaaaaaat ggataaaggt ttcgatacag ttattggtga gaacggagct
     3001 gagctatcag gaggagaaag acaaagatta tcaatagcca gagccttctt aaaagatgcg
     3061 ccgatattga tcttagatga gataacagca agccttgatg ttaacaacga gaaaaagatt
     3121 caagagtctt taaataattt agttaaagat aaaactgttg taatcatttc acatagaatg
     3181 aaatccatag aaaatgcaga caagatagta gttcttcaaa acggaagagt agaaagcgaa
     3241 ggtaagcatg aagagctttt acaaaaatca aaaatttaca aaaatttaat agaaaagaca
     3301 aaaatggcag aagaatttat ttattaggag gactacaatg gataataaaa aattaaaagt
     3361 aaaagattta gtaagtatcg gtgtttttgg cgtaatttat tttgccttca tgtttggagt
     3421 tggtatgatg ggcttgattc caatattgtt cttaatatac ccgacagtat tagccatagt
     3481 tgcaggaact gttgttatgt tatttatggc taaggttcaa aagccatggg cactatttat
     3541 atttggtatg atatcaccac ttgtgatgtt tgcagctggt catacctacg tagttgtggt
     3601 tttatcactt atagtaatga taatagcaga attaattaga aagattggta attataattc
     3661 atttaaatac aatatgcttt cttatgcaat cttcagcaca tggatatgta gctctttaat
     3721 gcaaatgctt ttagcaaaag aaaaatatat ggagtggtct ttgatgacga tgggaaaaga
     3781 ttatgttgat gtattagaaa agttaataac ttatcctcac atggctttag tagccttagg
     3841 tgctttctta ggaggaattc ttggagcata tataggcaag gctctattga aaaaacactt
     3901 tgaaaaagca ggcattgtat aatgcctact ttttcttcaa atgctccctt taatcccaat
     3961 ccgatctcta agatattagt cgtatttatc acaggcttaa ctgttatgca tagcataaac
     4021 atccgttttg agctggcaat tgtttgtctt attggtattt tgctttattt gaatggatac
     4081 aaaaaaacac ttttcaaatg gattatattg tgtgggatac tttactcctt gcctaatttt
     4141 atggtgttat ctgaattaaa ccctatagtt aagatgtttt tgagtatacc tattgttatt
     4201 agaatgttta ttttaccatt tatggcagca agctttatga taaagacctc ggatgtaggc
     4261 gcaataattt catcgatgga taagcttaag atttcaaaga atgtatccat acctattgcg
     4321 gttatgttta gattcttccc atcttttaag gaggagaaga aaaacatcaa aatggctatg
     4381 agagtaagag ggataagttt taaaaaccca gtcaaatatc ttgaatatgt ttctatgcca
     4441 ctactcatta tatcatctaa tatatcagat gacattgcaa aagcggcaga aacaaaggca
     4501 atagaaaatc caattgccaa gaccagatac attcgcgtaa agatacagct aattgatttt
     4561 gtttatgttt tagcggttgc tggacttatt gtgggaggct taatatggtt gaaataaaaa
     4621 atttaagtct tgattatggt gaagagcata tattagatga tatatcacta tccatagccg
     4681 agggagagtg cgtgctattt acaggaaaaa gtggaaatgg taagtcatct ttaataaatt
     4741 caatcaatgg actagctgta aggtatgata acgcaaagac aaagggcgaa ataat
//
LOCUS       DAVLWA010000111         4651 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLWA010000111 DAVLWA010000000
VERSION     DAVLWA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.111"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            97..555
                     /locus_tag="VYN80_001887"
     CDS             97..555
                     /locus_tag="VYN80_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1541742.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            568..1176
                     /locus_tag="VYN80_001888"
     CDS             568..1176
                     /locus_tag="VYN80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541743.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1247..1579
                     /locus_tag="VYN80_001889"
     CDS             1247..1579
                     /locus_tag="VYN80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541744.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1590..3005
                     /gene="ply"
                     /locus_tag="VYN80_001890"
     CDS             1590..3005
                     /gene="ply"
                     /locus_tag="VYN80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1541745.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            3888..4604
                     /locus_tag="VYN80_001891"
     CDS             3888..4604
                     /locus_tag="VYN80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1541746.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
BASE COUNT         1462 a          813 c         1036 g         1340 t
ORIGIN      
        1 aggaagtcta ataagttata gtccagtttt tactatactt tgatattcaa atatgttata
       61 ctatgattat caaagtaaat gaaaggagtt acgaatatga cgccagaaga aatgtacctg
      121 acagagcgat tagacgtaca gatagctcat tttttaaaga aaagcgttca acatcgtagg
      181 cgctataagg tattaaaaat aacagaaatc gtggcaggtt tcctcatagc tgtcttttgt
      241 gctattccta tgccaggtga tcgctaccgt ttgatttcgg ttgccttatc cagtctcggc
      301 ttgctgtgtg aggggattat caatttgtat aatgcaaagg aaaattggat ttcttaccaa
      361 aaaactgcgc aactcctgga gaaagaaaaa ttcctctatc aatgccaaac ggagaaatat
      421 gcaggaaaga ccaaggcttt tgccctattt gtcaagacat gcgaaggtct tatctcagag
      481 gagattaacc agtgggaaag tatccagtca aaagaagtgg cagctagtgc agatgctcca
      541 gttaaaaaag agtaggaggt agaggaaatg tctcaatcca gctacctgtc gcccttgctc
      601 tggttaaaaa aagaagccga taaggaaaag atgagcgcga cccagtgcca gatatttttc
      661 ttttactatc aaatgtttga gctcttattt gctagagaaa gcgacatgaa agacttatgt
      721 ctgggaacga aaggttttta tttctcgcag ttagagaaaa atttgctttc tggagtttcc
      781 cgatttctaa aaaacttgga ggggaaagta actctcaagg ctaaccaaga agtatcagct
      841 cgcaaagccc tttttctagc cttgacaact agccaatcag attggcagga gttagctcct
      901 gtttttgatt tttatcagac tatcgggagg cttgaaaatc cttctctctt gagttctcag
      961 gacagacaac atctgatgtg gatttaccag tcagctttgg agaaggatta tattgtcaag
     1021 gttattggcg acaagcattt tgtattgaag agacaagatg ctactaaatt gacagcgcgc
     1081 caaactcaaa ctttggaaat tctgagtcaa tcagaagact tggtcaatcc tgtctatgtt
     1141 acattaggag aaaagggggt gctcttgctt gattaagaga ggagatgttg tagctcttta
     1201 tttgcctttt ccgactatta gtagcgattt ggctgtgaag aatcatatgt atatctgtat
     1261 tgacaacagc atgactaaaa acaaagagtt ggttaaaaat cagaccttca aaccagctct
     1321 cttgaccaga cgtttggtca agaactttat gatagaagag ccggatctag ctcgtaatcc
     1381 ttttacaaga ccaaccttga ttgacttaga taaggtattt atgttggata atacggttat
     1441 tccgacttct tatctagcca gacggcgacg caatgtctca gaagaattgt acgaggaaat
     1501 tttggattac ttagtccaac cacggctgat ttcgctgaac aagtctgagt ttatgcaact
     1561 caatccagga acttattagg aggtagaaga tggcaaataa agcagtaaat gactttatac
     1621 tagctatgaa ttacgataaa aagaaactct tgacccatca gggagaaagt attgaaaatc
     1681 gtttcatcaa agagggtaat cagctacccg atgagtttgt tgttatcgaa agaaagaagc
     1741 ggagcttgtc gacaaataca agtgatattt ctgtaacagc taccaacgac agtcgcctct
     1801 atcctggagc acttctcgta gtggatgaga ccttgttaga gaataatccc actcttcttg
     1861 cggtcgatcg tgctccgatg acttatagta ttgatttgcc tggtttggca agtagcgata
     1921 gctttctcca agtggaagac cccagcaatt caagtgttcg cggagcggta aacgatttgt
     1981 tggctaagtg gcatcaagat tatggtcagg tcaataatgt cccagctaga atgcagtatg
     2041 aaaaaatcac ggctcacagc atggaacaac tcaaggtcaa gtttggttct gactttgaaa
     2101 agacagggaa ttctcttgat attgatttta actctgtcca ttcaggcgaa aagcagattc
     2161 agattgttaa ttttaagcag atttattata cagtcagcgt agacgctgtt aaaaatccag
     2221 gagatgtgtt tcaagatact gtaacggtag aggatttaaa acagagagga atttctgcag
     2281 agcgtccttt ggtctatatt tcgagtgttg cttatgggcg ccaagtctat ctcaagttgg
     2341 aaaccacgag taagagtgat gaagtagagg ctgcttttga agctttgata aaaggagtca
     2401 aggtagctcc tcagacagag tggaagcaga ttttggacaa tacagaagtg aaggcggtta
     2461 ttttaggggg cgacccaagt tcgggtgccc gagttgtaac aggcaaggtg gatatggtag
     2521 aggacttgat tcaagaaggc agtcgcttta cagcagatca tccaggcttg ccgatttcct
     2581 atacaacttc ttttttacgt gacaatgtag ttgcgacctt tcaaaatagt acagactatg
     2641 ttgagactaa ggttacagct tacagaaacg gagatttact gctggatcat agtggtgcct
     2701 atgttgccca atattatatt acttggaatg aattatccta tgatcatcaa ggtaaggaag
     2761 tcttgactcc taaggcttgg gacagaaatg ggcaggattt aacggctcac tttaccacta
     2821 gtattccttt aaaagggaat gttcgtaatc tctctgtcaa aattagagag tgtaccgggc
     2881 ttgcttggga atggtggcgt acggtttatg aaaaaaccga tttgccacta gtgcgtaagc
     2941 ggacgatttc tatttgggga acaactctct atccgcaggt agaagataag gtagaaaatg
     3001 actaggagag gagaatgctt gcgacaaaaa gaggcgatga tctctctgcg gatatctgaa
     3061 agtttatctc ttgcctagcg atttccattg gagtatgctc gtgatgttaa aatgaatcta
     3121 tcaaacagaa tgtgtaaaaa agtaaaaagg gtagccaatt ccttgatata caggggattg
     3181 attatccttt acttgtttta gatgattgga aattcaagaa cgacacgtat cggaaatatt
     3241 ttgctgttta tatctaatac ccatttatgg aaaaaatgaa attaaatatg aacaaatcaa
     3301 ttgctagcaa tggtttagaa gtgccagtgt actagtctaa cttctccatc ttgggaacac
     3361 gtaaattata ctcttcgaaa atctcttcaa accatgtcag ccctatccgc aacctcaaaa
     3421 cagtgttttg agcaacctga ggctagcttt ctagtttgct ctttgatttt tattgagtat
     3481 tagagatttg cttagaaact cttctattga tttcttagaa tacaatagcg cctataagaa
     3541 ctgttggatt tcaaagtatt tacttgaact atgatatgtc tggagtagac tgtcaacaaa
     3601 aggtgcagta tgattgtttt tgtcgcttat atctacaaac ttcaaacagt aatttgagct
     3661 gactttgtca aacttggctg aaactttcta tttgcagata atggtctagt ttgttttttg
     3721 aatgtggatg agtgtctgtt gataaaaaga tagaaaggca agaccatctt agcaggtaaa
     3781 gtgctatcct agcttgattt gctaataaaa tccatttcct ctagtgaaaa tcaaaaaaac
     3841 ttgtgttata ataagaaaga ttaaaatgtg aaaaaaggag attcctaatg ggacgtaaat
     3901 gggccaatat cgtagccaag aaaacggcta aagatggagc caactctaaa gtatatgcaa
     3961 aatttggtgt agaaatctat gtagcagcta aaaaaggtga tccagatcca gaatcaaact
     4021 cagctttgaa attcgttatc gaccgtgcta aacaagccca agtgccaaaa cacattatcg
     4081 ataaagcgat tgataaagcc aaaggaaaca cagacgaaac ctttacagaa ggacgttacg
     4141 aaggttttgg gccaaatggc tctatgctaa ttgtggatac tttgacttct aacgtcaacc
     4201 gtacagcagc caatgtccgt gcagcctttg gtaaaaacgg cggaaacatg ggcgcttcag
     4261 gttctgtttc ttacctcttt gacaacaaag gtgttattgt atttggaggt gaagatgcgg
     4321 acgcagtctt tgagcaattg ctcgaagcgg atgtggatgt ggatgacgta gaagcacaag
     4381 aaggtacaat cacagtttac acagcgccaa ctgaccttca caaggctatc gttgccctcc
     4441 gtgagtctgg tattgaagaa ttccaagtga ctgaattgga aatgattcct cagtcagaag
     4501 tggaattgtc aggcgaagac cttgaaacct ttgaaaaact ttacagcgtt cttgaagacg
     4561 acgaagacgt ccaaaagatt tatacgaacg tagatgggtt ttaatttaac ctatagaaca
     4621 tgatcctaag tgagaaaatc acttgggatt t
//
LOCUS       DAVLWA010000112         4596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLWA010000112 DAVLWA010000000
VERSION     DAVLWA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.112"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(58..1068)
                     /locus_tag="VYN80_001892"
     CDS             complement(58..1068)
                     /locus_tag="VYN80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEV1541747.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSIVLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1217..2386
                     /locus_tag="VYN80_001893"
     CDS             1217..2386
                     /locus_tag="VYN80_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1541748.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2383..3153
                     /gene="recO"
                     /locus_tag="VYN80_001894"
     CDS             2383..3153
                     /gene="recO"
                     /locus_tag="VYN80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1541749.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDDRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3150..4142
                     /gene="plsX"
                     /locus_tag="VYN80_001895"
     CDS             3150..4142
                     /gene="plsX"
                     /locus_tag="VYN80_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1541750.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4148..4381
                     /locus_tag="VYN80_001896"
     CDS             4148..4381
                     /locus_tag="VYN80_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1541751.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4427..4596
                     /locus_tag="VYN80_001897"
                     /pseudo
     CDS             4427..4596
                     /locus_tag="VYN80_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1347 a          882 c         1175 g         1192 t
ORIGIN      
        1 aacatatacc tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtgtta
       61 gaaaatgccg ataagggtct gcataccaag agtggtgagg atgatggcaa tccagcagat
      121 ggctccaaga acaatggatt ttccactgga tttgaccata gcgaccagat tggttttgag
      181 accgatagca ctcatggcca tgacaataag gaatttagag agttgtttga gaggggtaaa
      241 gaaactactg gacacaccta gagaggtgag tagtgtagtg aggagagagg caaggataaa
      301 gtaaaggata aaaagtggga agactttttt cagttgtagg ctttgcttgt tttcttgttg
      361 gcgactttgc cagtaggata gaaagagcgt gatagggata atggccaaag tacgtgtgag
      421 tttaacaatg gttgcagact cgagggtatt gctttggtaa agactgtccc aagcgctggc
      481 ggcagccgtt acagaggaag tgtcgttgac cgcagtccct gcaaagaggg cgaagccttc
      541 attggataga tgaagccagg tgccgagggt tggaaagatg agcgcagcca agacattgaa
      601 gaaaaagata acggaaatgg cttgggctac ttccttttcc ttagcatgga taacaggcgc
      661 tgtcgctgca atggcagaac ccccacagat agaagaacct actccaacca aggtagccag
      721 ttttgtatcc agtgcaaaga aacgctggaa gaggtaggca ataatcagag ctattgagat
      781 agtggacagg atgacaggga gtgaagattg gccaactgcg aagacctgcg agatattgag
      841 accaaaacca agcaagacaa cggcatattg gagcaacttc ttggaactaa aggtcaaacc
      901 agcatccagt tgtttatagg acgagagaaa gggatgtagg agcatgccta tgaaaatggc
      961 aaaaacgggc gcgccaatca cagggaagaa tcctcctaag taccaagata ggatggaaat
     1021 gagaaggcag gccaagatac ctgctccatt ttttgataga aatgacataa aaacctccaa
     1081 ataaaatcta ttaccattat agacctataa acaggaaaag taaaatagaa agtggaaagc
     1141 tattctctaa tgtatttttg cggtcggggg gcttttgtag tataatagag atacgttttg
     1201 aaagtaggag gtatctatgg acttaactaa gcgctttaat aaacagttag ataaaattca
     1261 agtttcgttg attcgtcagt ttgaccaggt tatttcggag attcctgggg tcttgcgttt
     1321 gaccttgggg gaacctgatt ttacaacgcc agaccatgtc aaggaggcgg ccaagcgagc
     1381 gattgatcag aaccaatcct actatacagg gatgagtggt ctgctgactc tacgtcaggc
     1441 agccagtgac tttgttaagg aaaagtacca actggactat gctcctgaaa atgaaatctt
     1501 ggttacaatt ggggcgacag aggctttatc tgcgactttg acggctattt tggaagaggg
     1561 agacaaggta cttttgccag ctcctgctta tccaggctat gaaccgattg ttaacttagt
     1621 tggggcagaa attgttgaga ttgatacgac tgaaaatggt tttgtcttga ctcctgagat
     1681 gttggagaag gccattttgg agcagggtga taagctcaag gcggttattc tcaactatcc
     1741 agccaatccg acaggaatta cctacagtcg agagcagtta gaggccttgg cagctgtttt
     1801 acgcaagtac gaaatttttg ttgtctgtga tgaggtttac tcagaattga cctacacagg
     1861 cgaagcccat gtgtctctag gaacgatgtt gagagaccag gctattatta tcaatggttt
     1921 gtctaaatcg catgccatga caggttggcg tttggggctg attttcgctc ctgcgacctt
     1981 cacagcccag ttaatcaaga gtcaccagta cttggtcact gccgcaaata ccatggcgca
     2041 acatgctgca gtagaagcct tgacggctgg taaaaacgat gcggagccca tgaagaagga
     2101 atatatccag cgtcgggact atatcatcga aaaaatgact gctcttggtt ttgagattat
     2161 caaaccagac ggtgccttct atatttttgc taaaattcca gcgggctaca atcaagactc
     2221 ctttgctttt ctgaaggatt ttgctcagaa gaaggccgtt gcctttatcc ctggtgcagc
     2281 ctttggacgt tacggggaag gctacgtccg cctatcttat gcagccagca tggagactat
     2341 caaagaagcc atgaaacgac ttgaggagta catgagagaa gcatgattca gtctatcacg
     2401 agtcaaggct tggtgcttta caatcgcaat tttcgtgagg atgacaagct cgtcaaaatt
     2461 tttacagagc aggttggcaa acgcatgttt tttgtcaaac acgctggtca gtctaagctg
     2521 gcgcctgtta ttcagccctt ggtgctggca cgatttctct tgcgaatcaa tgatgacgga
     2581 ctcagttaca tcgaagacta tcatgaggtc atgacttttc ccaagattaa tagtgacctc
     2641 tttgtcatgg cctatgcgac ctatgtggca gctcttgcag atgctagttt gcaggacaat
     2701 cagcaggatg ctcccttgtt tgcttttttg caaaagactt tggagttgat ggaagcaggc
     2761 ttggattatc aggttttgac caatattttt gaaattcaaa ttttgactcg atttggaatc
     2821 agcctcaatt ttaatgagtg tgtcttctgc catcgggttg gtcaggcttt tgacttttct
     2881 ttcaaatatg gagcctgtct ctgtccagag cattatcatg aggatgatag acgttgccat
     2941 ctcaatccca atatccccta tctgctcaat caatttcaag ctattgattt tgagactttg
     3001 gagaccattt cgctcaagcc tggaatcaag caagagctac gccaatttat ggatcaacta
     3061 tatgaagagt acgttgggat tcacctaaaa tcaaagaaat ttattgattc cctagcagac
     3121 tggggacaat tactaaaaga ggaaaagaaa tgaaaaaaat cgcagtagat gccatggggg
     3181 gcgattacgc acctcaggcc atcgttgagg gtgtcaatca agccctatct gacttttcag
     3241 atatcgaggt tcaactttac ggagatgaag ctaaaatcaa gcaatatctg acagcgacag
     3301 agcgcgtcag cattatccat acggatgaga agattgattc ggatgatgaa cctacgagag
     3361 ctattcggaa taagaaaaat gccagtatgg tattggcagc caaggctgtc aaagatggtg
     3421 aagcagacgc tgtcctttcg gctgggaata caggtgcctt gttggcagca ggattcttca
     3481 tcgtgggtcg tatcaagaat atcgaccgtc ctggactcat gtctaccttg cctaccgttg
     3541 atggaaaagg ttttgacatg ctagaccttg gtgccaatgc agaaaataca gcccagcacc
     3601 tccatcaata tgcggttcta ggttccttct atgctaaaaa tgtccgtggc attgcgcaac
     3661 cacgcgttgg tttgctcaac aacggaacag agagtagcaa gggcgacccg cttcgtaagg
     3721 aaacttatga attactggcg gctgatgaaa gtttgaactt tatcggaaac gtggaagcgc
     3781 gtgatttgat gaatggcgtt gcagatgttg ttgtggcaga tggtttcacg ggaaacgctg
     3841 tgctcaaatc catcgaaggg acagctatgg gaatcatggg cttgctcaag acagctatta
     3901 caggtggtgg tcttcgagcg aaactaggtg ccctccttct caaggacagc ctcagtggtt
     3961 tgaaaaaaca gctcaattat tcagatgttg gtggagcggt cttgtttggt gttaaggcac
     4021 ctgttgtcaa gactcatggc tcaagcgatg ccaaggctgt ttatagtacg attcgtcaga
     4081 tccgtaccat gctagaaaca gacgtggttg cccagactgc gcgtgaattt tcaggagaat
     4141 aaaagagatg acagaaaaag aaatttttga ccgtattgtg accattatcc aagagcgaca
     4201 gggagaggac tttgtcgtga cagaatcctt gagtctgaaa gacgatttgg atgcggattc
     4261 tgttgacttg atggagttta tcttgactct ggaagatgaa tttagtatcg aaatcagcga
     4321 tgaagaaatt gaccaactcc aaaacgtagg agatgtggtt aaaatcattc aaggaaaata
     4381 gcaatcggag ttccaagtca acggaagtag atggttttta gaaatgagaa atatcggaca
     4441 agctggaaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
     4501 acaaactcca cgtaaatcca gcaaactcaa gccgctaaca gttgaagata aagcctgtaa
     4561 tcatgcgcta tctaaggaga gaagcaaggt tgagaa
//
LOCUS       DAVLWA010000113         4519 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLWA010000113 DAVLWA010000000
VERSION     DAVLWA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.113"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            96..329
                     /locus_tag="VYN80_001898"
     CDS             96..329
                     /locus_tag="VYN80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1541752.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VYN80_001899"
     CDS             329..1663
                     /locus_tag="VYN80_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1541753.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VYN80_001900"
     CDS             1673..1927
                     /locus_tag="VYN80_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1541754.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VYN80_001901"
     CDS             2023..2418
                     /locus_tag="VYN80_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1541755.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2831..3280
                     /locus_tag="VYN80_001902"
     CDS             2831..3280
                     /locus_tag="VYN80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1541756.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3267..3824
                     /locus_tag="VYN80_001903"
     CDS             3267..3824
                     /locus_tag="VYN80_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541757.1"
                     /translation="MARTELPDQIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDSLIYGLLKSEWEE"
     gene            3826..>4519
                     /locus_tag="VYN80_001904"
     CDS             3826..>4519
                     /locus_tag="VYN80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1541758.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKE"
BASE COUNT         1386 a          815 c         1050 g         1268 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcaacct gcggctagct ttctagtttg ctctttgatt
       61 ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagctgg
      181 gttttaaacc ccttgccaat cccttgatgc gcaatacagt tggtcgtaaa gtatcactta
      241 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
      361 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
      421 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
      481 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
      661 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
      721 gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgata gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc ctatggagat
     1321 tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
     1441 taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga ccaaggacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gcaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt cgcgcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaataaaag tgtctttttg
     2581 ctttaaagac gagagttgcg ggcaactgcc cgaaattggg tggtaccgcg gataaacaca
     2641 ttcgtccctg tcatgtagat ggcagggctt ttcttttacg tcttagtcaa agggggttgt
     2701 tatgaagcaa tcttttaaca caagtaaact atattatggt tttcctgttt ttattttagg
     2761 atatcaagat tagaattttg gctataacat cacgacctgt agttcctctt atagtctagg
     2821 agattgggtt gtgattggag tcgttgcgag agagaatgcc gcagagcaga tcaaacagta
     2881 tcaaaaattt actgtgaata tttctgatga aacttctatg cttgcgatgg agcaggctgg
     2941 ttttatcagt catcaggaga aattggaacg tttgggagtg cattatgaaa tttctgaacg
     3001 aactcagact cctattttag acgcctgtcc acttgtttta gattgtaggg tagataggat
     3061 tgttgaggaa gacggtattt gccacatctt tgccaagatt cttgagcgac ttgttgcccc
     3121 agaactcctg gatgaaaagg gacattttaa aaatcaactg tttgccccaa cctatttcat
     3181 gggagatgga tatcagcgcg tttatcgcta tctggataag cgtgtagata tgaagggcag
     3241 tttcatcaaa aaagcgagga agaaggatgg caagaactga gctgccagat caaatcgaaa
     3301 cagaacgtct cgttttacga gtccgtactg tggcggatgc tgaggatatc tttgactatg
     3361 ctagtttgcc agaggtcgcc tatccagcag gttttcctcc agtcaagacc ttggaagatg
     3421 agatttatta tctggagcac attcttccgg agcgtaatca aaaggaaaat ctcccagcag
     3481 gctacgggat tgtcgtcaaa ggaacggata agatcgttgg ctctgtcgat ttcaaccatc
     3541 gccatgaaga tgatgtgctg gaaatcggct ataccttaca cccagactat tgggggcgag
     3601 gttatgtgcc agaagctgcg cgtgccttga ttgacttagc ctttaaagat ttgggtcttc
     3661 acaagattga actaacttgc tttggatata accttcaaag taaacgagtc gcggaaaagc
     3721 ttggctttac cctcgaagct cgcataagag accgcaaaga tgctcaaggc aactgctgtg
     3781 acagtctgat atatggcttg ctgaagagcg agtgggagga ataagatgag cgatgtaaaa
     3841 gaggaagtaa gctctctatc tgagaagcag ttacgccaaa ttgatgttga gtatgctgaa
     3901 ttaaatgata gtgatattat cgagcgatta gcttatttag aaattaacaa caatgaaaaa
     3961 cgtattgtca tctctgatat tgaaccaacc aaggaaataa tgtcagtctc ggaccaaatt
     4021 tttgagattc aaaagaattt tcaaaaaatc aagaatatgt tcgaattgtt tatttcagat
     4081 gtctcggatt ttttaagtat taaaaataaa cttgagtcaa aggaattaga aattgaagag
     4141 gcagatgtaa atcgtttcat gattcattta ttatccagtg gaaaattgtt tgtcgatttt
     4201 aacgaaaacc aaattaaaca aaaatattcc aaagatagtg aggagtttga ttgtatacat
     4261 ggttttgcaa gctatcagta tgataccaat tttgcttatc gattttgcca ttctttgaga
     4321 aattattcgc aacatactga cttaccaata aatgaggtaa aagctgttag cccagatgat
     4381 gagactgtta taatagattt ctatattgat ttagattatc ttttaaattc caactttaaa
     4441 tggaaaaaat tgaaagggga gttgataaaa ctaaaccaag aaacatctaa gattgacgct
     4501 atagctctag tgaaagaat
//
LOCUS       DAVLWA010000114         4145 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLWA010000114 DAVLWA010000000
VERSION     DAVLWA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.114"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(45..596)
                     /locus_tag="VYN80_001905"
     CDS             complement(45..596)
                     /locus_tag="VYN80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541759.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(946..1770)
                     /gene="nadE"
                     /locus_tag="VYN80_001906"
     CDS             complement(946..1770)
                     /gene="nadE"
                     /locus_tag="VYN80_001906"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1541760.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(1767..3227)
                     /locus_tag="VYN80_001907"
     CDS             complement(1767..3227)
                     /locus_tag="VYN80_001907"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1541761.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3343..3831)
                     /locus_tag="VYN80_001908"
     CDS             complement(3343..3831)
                     /locus_tag="VYN80_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541762.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(3821..4030)
                     /locus_tag="VYN80_001909"
     CDS             complement(3821..4030)
                     /locus_tag="VYN80_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541763.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1168 a          990 c          781 g         1206 t
ORIGIN      
        1 atttacaata aatagttgac ttcgatagaa acagaaaata gaattcaaca ttcttcccta
       61 cttataccat aataaataag atccgcaaga tagccttttc tctctacacc attaacaatg
      121 gtttgtagat aggacattcc agccttttcc atgacacgac ctgaagctgg gttgagactg
      181 gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa aacaaaagtc caagatagct
      241 ttcaaagtct ctgtcatcat accatttccc cagtaagcct tgcctaacac atagccaatt
      301 tcacagctta aatcagcctc gtctatctta acaatgctga tatctcctat tacttgctct
      361 gggttttctt ttagacaaat ggcccattta taatagttga gattagtata ggaagcaacc
      421 caattgcaaa tcgagtttcg agtgatttcg acatcaggat ggggatccca ggtaacatag
      481 gtcagattct cagcggatga agcccaattt tgaaacatgg cttctgcatc actctccaca
      541 aatcttctta aaatcaaacg ttctgtctgt aatatttgcg taccgataga tttcatgatg
      601 ctacctcact tccttatttc tatactcgta gtaaaaaggc tcaactcagt aacagctaga
      661 aaacgagttt gtcttctaat ttcttgacgc agttgctgcc tggtttgaaa tgcgctctag
      721 caagcttttt caaacctagt catcgttgca ggggtaagac gatgaaatcg aactcttcga
      781 gttactgaat ctgcctcatt ttcttatttc taaactcggg gttcaagatc ccccagacct
      841 tctctcactt tcttatttca agatgagagg ctgaaaacct ccagtggagg tttttcttgc
      901 tttttaattt cagggcaaga agctaaaaag gtcccccgga cctttttact cccaaaagtc
      961 atcaaatacg gtgatgggta agtggcgttt gtgttggcct ttgtgccacc agttttcaat
     1021 ggtcgcttga gcttctgggc tgattgtttt gccttctagg tagtcgtcaa tctctgcgta
     1081 ggtgactcca agtgcgactt cgtcagctag gcctggttta tcttcttcta ggtctgccgt
     1141 tgggattttt tcataaaggg ctggctctgc gccaagtttc tgcaagagct gttttccttg
     1201 gcgtttattg aggcggtaaa gagggagaat atccgcaccg ccgtcaccaa acttggtaaa
     1261 gaaacctgtg atattttccg cggcgtggtc tgttccaatg accgctccgc tatgggaacc
     1321 agcaagggca tactgagcaa tcatacggca acgtgccttg atattcccct tgttgaagtc
     1381 tgaaacagga ctacctgtcg cttcaactgc agctgtcatg gcatcagctg attccttgat
     1441 attcacaacc aagctgacat ctggctggat gaaggctagg gctttttgag catctgcttc
     1501 atcagcttgc actccgtatg gcaggcggac agcgataaat ttgtagctat cgtctcccgt
     1561 ttcagctcgc agttcttcca tagctaattg cgccaaacgt cctgccaagg ttgagtcttg
     1621 tcccccagaa atccctagta caaaggtttt taggaaggga tgttttttca gatatctttt
     1681 taagaaatca atagaacgac ggatttcttc ctgggcatca atcactggtt tgacacccag
     1741 ctcttggata atcgtttctt gcaaactcat tcttcttctc cttcaccaag ggcttccttg
     1801 cgcatcttat caatcaagtc catcttatct tgccatacat cacgcgccaa atccactgga
     1861 tagtgctgcg gattgagcac acgcttatac tcatcccaca acttgtcaaa ttccttacgg
     1921 gcataatcct gaatgtcagt caaactaggc aagttgtaaa ctaatattcc ttctttgaag
     1981 atatccacca agagaggaac ggcatcaaaa ttacgaaccg tcttcttgat gtatgtatag
     2041 gtcggatgga acatcttgat ttctgtcatg tcgctaatat ccacaccatc ataagtgata
     2101 tagtcgcctt ctgacttgcc tttttcacga ctggtaatgc gccacacctg cttcttacct
     2161 ggcgtagaaa ctttttcagc attattagac agcttaatcg tattgcgcat ctgaccagtt
     2221 tcatcttcga ttgcaacaat cttgtaaacc gccccaagag ccggctggtc ataggctgta
     2281 atcagcttgg tacccacacc ccagacatca atcttggcct tttgcatctt gaggttaagg
     2341 atggtatttt catctagatc attagaagca taaatcttag cctctgtaaa tccagcctca
     2401 tccagttgct gacggacttt cttagaaatg taggcaatat ccccagagtc aatccgcaca
     2461 cccataaagt taatctgatc acccagctca cgcgccacct gaatggcagc tggtacaccg
     2521 atgcgaaggg tgtcataggt atccacaaga aagacacaat ttttgtgggt cgcagcgtaa
     2581 gccttgaaag cttcatagtc attgccataa acctgtacca aggcatgggc atgggttccc
     2641 aaaacaggaa tgtcaaagag cttacccgca cgcacgttgc tagttccatt ggcgccacca
     2701 atcacagctg cgcgtgttcc ccagatggcc gcatccattt cttgagcccg acgtgtccca
     2761 aactccatca agggttcatc ttcgataacc gaacgaatac gagctgcctt cgtcgccacc
     2821 aaggtctggt agttgacgat gttcaaaaga gccgtttcga ccaactgaca ttgggctaga
     2881 ggtccttcca cctgcacaat cggttcatta gcaaaaacca aatccccttc ttgggcagaa
     2941 cgaacggtca actccaactt gaaattgcga aggtaatcca agaacgcccc atgataacca
     3001 agcgactcca aataggctat atcactatct gaaaaacgca agtcttcaag atagttcaca
     3061 attctttcca aacctgcaaa aaccgcatag ccgttcttaa aaggctgttg gcggaaatac
     3121 acctcaaaga ccgccttctt attgtgaatc ccttggtcaa agtaaacctg catcatgttg
     3181 atctggtaca agtccgtgtg caatgtcaaa ctatcatctg gatacatact tttcctactt
     3241 ccttagctag aaacccatga aaattttcaa gaactttcat gtattccaat aaattagtac
     3301 tattatatca cattttagct ggattgagaa aagagtaaca agctattctc cactctccaa
     3361 ttcatccata tcttgttcaa attttttctg agcccattcg ccatagctct taagaccaag
     3421 attgccaata aagatccacg gaaggtaaat gatataagta atgacccaag cagacaggta
     3481 tttaaaattc aaaggattgt gctgataaat ttctatgttg aattgataat tctgcaacat
     3541 caaaagagcc gtaatagcca aggttaggaa aaaacaaccc aaaatcgtaa aatgaaaacg
     3601 actatagtag gtcactccca gataacgggc acgattgaaa aagtaaaatg tccctatgat
     3661 gataacgatt agcagcatat tagaattaaa aaggcttggt gctaatactg aaatgatata
     3721 agataggagc gacaaagcga tgcagatata gaaactttca gagcccgctt tattgaacag
     3781 ttgttcttct ctttcgtcta gtaattgata ataataaaat ctatttttca tcttcttcct
     3841 cccaaaatag ttggtctagg gttttcccta aacatctgca aatggactgg cagagcgaga
     3901 gactgggatt gtatttcccc gcttctatca aaccaatagt ctggcgcgtc accccgacag
     3961 cctctgccag ttgaccttgt gttaaatcac gctctacccg agctaatttt aattttaaat
     4021 ttttagccac cttcgtcctc cttatagttt taatactcat ctactcttaa aaaatccaaa
     4081 accaacacaa gctatcagct atttattata accccattaa tttgcactcc agtattatct
     4141 tcagc
//
LOCUS       DAVLWA010000115         4080 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLWA010000115 DAVLWA010000000
VERSION     DAVLWA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.115"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            23..805
                     /locus_tag="VYN80_001910"
     CDS             23..805
                     /locus_tag="VYN80_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1541764.1"
                     /translation="MIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALCEYLNVSKAT
                     LTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLRKTYTVLDEV
                     QLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDMILWSSLLLN
                     ENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDELLLLASTDHL
                     SYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            810..1511
                     /locus_tag="VYN80_001911"
     CDS             810..1511
                     /locus_tag="VYN80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1541765.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1536..2453
                     /locus_tag="VYN80_001912"
     CDS             1536..2453
                     /locus_tag="VYN80_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV1541766.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2481..4022
                     /locus_tag="VYN80_001913"
     CDS             2481..4022
                     /locus_tag="VYN80_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1541767.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
BASE COUNT         1325 a          628 c          793 g         1334 t
ORIGIN      
        1 actgcaaaaa gaactaattc caatgataga agctaactta ccaaatatgg catatgctga
       61 aaaagacatt gctaaattct tcttaaaaca gcaacctctg aatgattatt catctaaagc
      121 attgtgcgaa taccttaatg tatccaaagc aacattgact cgatttgcga aaaaatgtgg
      181 ttttaaaggt tttagacaat tcattttcaa ataccaagag atgattcatg agaaagaaaa
      241 gttggcatta tatacagagg caacagaaaa agttttatcc gactatgagg aaatgttgag
      301 aaaaacttac acggttcttg atgaagttca acttgagcgt attgctgaga tgatagaaac
      361 tgctgagcgt gtatatctct acggtaaagg aagttctgtt cttgctttac aagaaatgaa
      421 gatgagattt atgcgtctcg gagtgattgg tgaagtatta tcagacgagg atatgatttt
      481 gtggagtagc ttactactta atgaaaattg ccttgtcatt ggagcatcca tttcaggtca
      541 aactgatatt gtactagaag gtctacaaaa agctgcagat aaaggcgcta aaacagtttt
      601 aatgactaca agaaaatttg acgaagaaga ttgtttcttt gatgaactat tgttattagc
      661 ttcgaccgat catctctcgt atggcaatcg catatcacct cagtttccaa tacttttaat
      721 tacagactgc ttattctcta attatctgga aagtccagag agacaatatt attacaatca
      781 aactattatc cataaggagg aataataaaa tgagccaaat ttggactaaa gaaaaattta
      841 taagccaagt tcaaggtgga gtcattgttt cttgtcaagc tttacctggt gaagcccttt
      901 ataatgaaga atttagcttg atgcctttta tggctaaagc agctttagag gcaggagcag
      961 tgggcattcg cgcaaattct gtgcgtgata ttaaagcaat tcagaaagta gtagatttac
     1021 caataattgg aattatcaaa agggattatc cacctcaaga accatatatt actgctacga
     1081 tgaaagaagt agatgaactt gtagaatgcg gaacaacagt cattgcattt gatgcaactt
     1141 taagaccaag atatgatggc ttagttgtca gtgaatttat caaaaaaata aaagaaaaat
     1201 atccgaatca attgctgatg gcggatgtaa gtaatttaga tgaaggtctc tatgcattta
     1261 aatcaggcgt tgattttgtt ggtacaacat tatcaggtta cacaagtaca agtgtacaat
     1321 cagatgagcc tgattttgaa ctaatgaaaa aattggctga ttttaatatt ccggtaattg
     1381 ccgaaggaaa aattcattat ccagaacaat taaaaaaagc ttatagttta ggtgttacca
     1441 gtgtagtcat tggtggagcg attacacgtc caaaagaaat tgctcagcga tttattaatg
     1501 tcatcaaata aaaaacaata taaaggagaa atctgatgaa agatttaact aaatacaaag
     1561 gcgttatccc tgcattttat gcttgctatg atgaaaatgg tgaaattagc caagatcgtg
     1621 taaaatctct ggtacaatat ttcattgaca aaggtgtaaa aggtatctat gtaaatggtt
     1681 cttcaggtga atgtatttac caaagtgtag aagatcgtaa acaaattatt gaagctgtta
     1741 tggaagttgc taaaggtaaa ttaacagtta tcaaccatat tgcatgtaat aacacgaaag
     1801 atagtatcga attggcaaaa cattcagaaa gtgttggagt cgatgctatt gcagctatcc
     1861 cacctattta tttcaaattg ccagagtatt caatcgcagc atattggaat gcaatgagtg
     1921 aagctgcgtc aaatacagat tttattatct ataatattcc acaattggca ggggttgcgt
     1981 tgactggtag tttgtatgca acaatgcgtc aaaatcctcg tgtgattgga gttaaaaatt
     2041 cttctatgcc tgtacaagat attcaaatgt ttgtagctgc aggtggagaa gattacattg
     2101 tattcaatgg tccagatgaa caatatcttg gtggtcgctt gatgggagca gaagctggta
     2161 ttggtggtac ttatggcgtt atgccagatt tgttcttgaa attggaaagt ttgattcaag
     2221 aacgagattt agatacagct aaaaaacttc aatatgctat caatgaagtt atctataaga
     2281 tgatatcagg taaggcaaat atgtatgctg tagcaaaaga agttttgcgt ctaaatgaaa
     2341 aacttgattt aggttctgtt cgtcaacctt tagaagcatt ggcagaaggt gacttggaag
     2401 ttgcaaaaca agcagcagaa cttattcaac aagcacgaaa agaattttta taataaataa
     2461 aatcaatatg gagatatgtt atgggcacaa caggatttac aataattgac ttaattatct
     2521 tgattgttta tttacttgcg gtgttggttg caggtatcta tttctctaaa aaagagatga
     2581 aaggaaaaga gttctttaaa ggagatggtt cggttccttg gtatgttact tcggtatcca
     2641 tttttgccac aatgctcagt ccgatttcct tcttgggact cgctggtagc tcttatgcag
     2701 gtagctggat tttatggttt gctcaattag ggatggtagt agctattcca ctgacaattc
     2761 gttttatctt acctatcttt gcacggatag acatcgatac ggcatatgat tacttggata
     2821 aacgttttaa ttctaaagca cttcgtatta tttcagcact cttgtttatt atttatcaat
     2881 tgggacgtat gtctatcatt atgtacctcc catcagctgg tttatcagta ttgacaggaa
     2941 ttgacatcaa tattttgatt attttgatgg gtgtagttgc aattgtttat tcttatactg
     3001 gtggtctaaa atccgtatta tggacagact ttattcaagg tgtgattctg attagtggtg
     3061 tcgttttagc tttatttgta ctgattgcta atattaaagg tggctttggt gcagtagcag
     3121 aaacattagc aaacgggaaa ttccttgctg caaatgaaaa acttttcgat cctaacttgc
     3181 tttcaaactc catcttttta attgtgatgg gttcaggctt tacaatcttg tcttcctatg
     3241 cttcatctca agatttggtt caacgtttta ctacaacaca aaatattaag aaacttaata
     3301 agatgttgtt cacaaacggt gttttgtcac ttgcaactgc aacagtcttt tacttgattg
     3361 gtacaggctt gtacgtattc tatcaagtac aaaatgcaga tagtgcagct agcaatatcc
     3421 ctcaagacca aatctttatg tactttattg cataccagtt accagtaggt atcacaggtt
     3481 tgatcttggc agcgatttat gcagcatctc aatcaactat ttcaacaggt ttgaactctg
     3541 ttgcaacttc atggacattg gatattcaag atgtcatttc taaaaatatg tcagacaatc
     3601 gtcgtacgaa aattgcacaa ttcgtatctc tagcagtagg tttattctca attggtgttt
     3661 ccattgtcat ggctcactca gatattaaat ctgcatacga atggttcaat agtttcatgg
     3721 gacttgtact tggtctactt ggtggtgtat ttattcttgg atttgtttct aaaaaagcaa
     3781 ataaacaagg tgcttatgca gcgctgattg tatcaaccat cgtcatggta tttattaaat
     3841 acttccttcc tccaacagct gttagctact gggcatattc attgatttca atctctgtat
     3901 cagtagtttc aggttatatt gtatctgttc ttactggaaa taaagtatct gcacctaaat
     3961 atacaacgat tcatgatatt acagaaatta aagcggattc aagttgggaa gttcgtcact
     4021 aatagaaaag agaaaataat atgattattt ctgaacaatc tgattttaaa cgatatgctt
//
LOCUS       DAVLWA010000116         4028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLWA010000116 DAVLWA010000000
VERSION     DAVLWA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.116"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            10..963
                     /locus_tag="VYN80_001914"
     CDS             10..963
                     /locus_tag="VYN80_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1541768.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1040..3028
                     /gene="uvrB"
                     /locus_tag="VYN80_001915"
     CDS             1040..3028
                     /gene="uvrB"
                     /locus_tag="VYN80_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1541769.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3039..3602
                     /locus_tag="VYN80_001916"
     CDS             3039..3602
                     /locus_tag="VYN80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541770.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3568..>4028
                     /locus_tag="VYN80_001917"
     CDS             3568..>4028
                     /locus_tag="VYN80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541771.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT         1170 a          712 c          937 g         1209 t
ORIGIN      
        1 gacggggata tgaagaacaa aagaatattt aaagacttcc aagcttcaaa aatgagttta
       61 aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct
      121 tttactttgt atggtattgg cttgttagct ctcatcggac ttgctagaaa ttttggagag
      181 gctggtcaaa atcttgcaag ctacttgcag accttgcatc agagcttgac ggataaaaca
      241 agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc
      301 agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat
      361 ttcctcagca atcttctgaa aggatttagt ctaggtctgg cactttttct tctgaccttg
      421 ttaggtttgg tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct
      481 cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg
      541 gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt
      601 cttatatcta gcctgttctt taccctgctt catatgggca attctggcct cacccctcta
      661 tctctagtaa atctcttttt attcggagtt gccatggctc tttaccttct caaaactgat
      721 acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt
      781 gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc
      841 aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg
      901 gtattactac tgctgattgt ctatcttgcc aataaattaa agaaagaaaa tgaaaggatg
      961 tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc
     1021 acatggagag aggattctta tgatcaatca cattacagat aatcaattta aactagtatc
     1081 aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga
     1141 ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat
     1201 gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct
     1261 ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt
     1321 cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat
     1381 tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc
     1441 ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg
     1501 ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg
     1561 tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgtaatgata ttgatttcca
     1621 acgcggaaga tttcgcgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga
     1681 acatgccttt cgagtagaat tttttggaga cgaaattgac cgtatccgtg aggttgaggc
     1741 tctgacaggt caggtattgg gagaggtgga tcatttggcg attttcccag ctactcactt
     1801 tgtgaccaat gacgaccata tggaagttgc cgttgccaag attcaagcag aattagaaga
     1861 gcagttagct gtctttgaga aggaaggtaa attgcttgaa gcccagcgct tgaaacaacg
     1921 gacagagtac gatatcgaaa tgctgcgcga gatgggctat acaaatgggg ttgaaaatta
     1981 ttctcgccac atggatggac ggagcgaagg agagcctcct tatacgcttc tcgacttttt
     2041 cccagatgat tttttgatta tgattgacga gagtcatatg actatgggac aaataaaagg
     2101 catgtacaat ggagaccgtt cgcgtaagga aatgctagta aattatggtt tccgtttacc
     2161 gtctgccttg gacaatcgtc ctctccgtcg tgaggagttt gaaagccatg tgcatcagat
     2221 tgtttacgta tcagcgacac ctggtgacta tgaaaatgaa cagactgaga cggtgattga
     2281 gcaaatcatt cgaccgacag ggcttttgga tcctgaggtg gaagtccgtc cgactatggg
     2341 acagattgat gatctcttag gtgaaatcaa tgcccgtgtt gaaaagaatg aacgaacctt
     2401 tatcaccacc ttgaccaaga aaatggcaga ggacttgacc gactatttca aggaaatggg
     2461 catcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg
     2521 tgatctccgc ttgggtgtct ttgatgtctt ggttggaatc aatctccttc gtgaagggat
     2581 tgacgttcct gaggttagct tggttgccat tctcgatgct gacaaggaag gtttccttcg
     2641 taacgagcgt ggactcatcc agaccattgg gcgtgccgcc cgtaacagtg aaggtcatgt
     2701 catcatgtat gcagatacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg
     2761 ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa
     2821 gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga
     2881 agtcgacatc aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga aactagaaaa
     2941 acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat
     3001 gatgttggaa gtcaaggcct tggattaggg gaattagtat gatttattta agaaagttaa
     3061 agaaagaaga tttgatgtct ttatgggaaa tggcttattc acagcttaat ccagtttgga
     3121 aacagtatga tgctccctat tatgatgatt atcagtattt ttcaaatttt aaagaattcg
     3181 aactacaaaa atcagaatcc attttaagca actcaaatcg ccttggtatt tttgttgatg
     3241 ataaactagt tgggactgtt tcgcgttatt gggtatgtaa agaaacaaga tggatggaat
     3301 tgggaattgg tatttatgat aaaaaattct ggaacactgg tattgggaaa gttgctatgt
     3361 tgcagtggat agataggacg tttcaggatt acttggagtt ggagcatctg gttttgacaa
     3421 cttggtcagg aaatcttggt atgatgaaac ttgctgaaaa attaagaatg aaaaaagaag
     3481 ctcatattcc aaaagttcgt tattatcaag gtaaatattt tgatagtatt aaatatggta
     3541 ttttgagaga agactgggag aaaataaatg acggttatta tcaaatcaat ggaaactcct
     3601 gaagagatag aaggcaaatc cctcgttcac tggcaaacgt ggagagagtc ttatgatgac
     3661 cttttgcctg cggaatttca ggatacaatg acattagaaa gatgtcgatt ctttagtcaa
     3721 aagtatccag aaaatacatt gattgcgatg gatggcgtga aggtggttgg ttttatcagt
     3781 tatggcaatt ttcgtgatga gactattcaa gctggtgaaa ttattgcttt atatgtttta
     3841 aaagactatt atggaaaagg tatcgcacaa aagttaatga aagcagcttt gactgctctt
     3901 aatcattttt ctgaaatttt cttatgggta ttgaaagata acaagcgcgc cattgctttc
     3961 tatcaaaaaa tgggttttac ttttgatgga caagaaaaaa tacttgaact tggaaagcct
     4021 ataaagga
//
LOCUS       DAVLWA010000117         3972 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLWA010000117 DAVLWA010000000
VERSION     DAVLWA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.117"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..152
                     /locus_tag="VYN80_001918"
     CDS             <1..152
                     /locus_tag="VYN80_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541772.1"
                     /translation="DVVSSKHCFEVVDRTDEVSNHTHGKATLTWFEEIFYEYKSKTFV
                     NRDTL"
     gene            complement(464..625)
                     /locus_tag="VYN80_001919"
     CDS             complement(464..625)
                     /locus_tag="VYN80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1541773.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(610..828)
                     /locus_tag="VYN80_001920"
     CDS             complement(610..828)
                     /locus_tag="VYN80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1541774.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(926..1711)
                     /locus_tag="VYN80_001921"
     CDS             complement(926..1711)
                     /locus_tag="VYN80_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541775.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1713..2531)
                     /locus_tag="VYN80_001922"
     CDS             complement(1713..2531)
                     /locus_tag="VYN80_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1541776.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2533..3525)
                     /locus_tag="VYN80_001923"
     CDS             complement(2533..3525)
                     /locus_tag="VYN80_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541777.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1324 a          873 c          705 g         1070 t
ORIGIN      
        1 ctgacgtagt cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta
       61 accatacgca cggcaaggcg acgttgacgt ggtttgaaga gattttctat gagtataagt
      121 ccaaaacttt cgttaaccgg gatacattat aagcacattc ataccatcaa aaatagtagc
      181 aataaacttg aacaactacc agaaacacga aaacaggcat aattgatgaa atatcagcgc
      241 tattaaacaa tataaataac cgctaagaat aaggcttaac ggttatcttc tacctatcta
      301 ttttttcaac tcctcagtcg tttatctacc tataacctag gctttacgag tgccttgctc
      361 taccaactga gctaaatcgg cgattacact tacagtatgg cactctgaga atagatgtca
      421 agtaatatta ttcaagcttt ttaatcaaaa tctgacaagg attttacgga ttccatagct
      481 gagaaaaaat ctctaacttt ttaaagccaa gacctcgata aaagtcattt gttcgatcat
      541 aatctttatt agaaccttct gccactgttt tgacctgcag ataaccaact tttttacgag
      601 cttcactctc taaagtagca agcaattggc tcccaatttt tctaccttga taagcttttt
      661 tacgccgaga caatcaatct ctgcacaatc ttcactcgaa taggataacc ttacaaatcc
      721 agtcaaatca ctctcctgat aggcggtcca aacttgcagt gtcgtggttc cttctatata
      781 ggcttgtgtg ctttctggga ttccaaacca ttctggcaaa tccttcaaaa cctcaacgac
      841 aactgccttt tttgattttc atccttgact tcttcaatta caaacatcat aagttccttt
      901 cttgatttta gtcttacttt agcttttacg aacccgcact ttcgtaggaa tctaagccct
      961 tatcccaaag tttaagggaa ataacaaaga aaaccagaga aatcaacatc aaacctccta
     1021 cgttaaagaa cacatccttt tcctgtaaga aatagctagc tggatagtag gctgtaaagg
     1081 cgaaaggcac gataaagcta atcaaccaac gaagaagaga attgtaaata gaaatcggat
     1141 acttagcaaa gtcattgaac atatagaaga tgtaaatcat ggcgcctgac tgcttagtcc
     1201 aaaaggcgat actggctgtt gcgattttaa gagaagtata aatcaaggtc gcaaaaggaa
     1261 tacaaactag gaaaagcagg aattttggaa gagtccaaac aatgctggtc actgttgttc
     1321 ccaataaaat accaccgact aagagttcac ccaaggcatc aatctgaaag gtttcaacta
     1381 ggatgtgaaa gagaggattg atgggacgag tcagatactt gtcaaactct ccttttcgga
     1441 ctaggcgttg ccctagtgcc cagagattgt caaaaaagag atggtccatt cccttgggaa
     1501 tcaaggaaaa tccataaatg aaagctatct cttgaaaggt ccagccttct aggaatggaa
     1561 tatgttgaaa gatgacattg agaaacaaga gattcaagcc ttgagccaga aagactccca
     1621 agacaccaac cacaaaatcc accttatatt ccatgatttg tttgatgtat tgtctgataa
     1681 aaatcagatg cattcgttga tattttttca tactaacctc cttgaatggt gataaaggac
     1741 tggacccgtt tccaaattaa ctgagacaat cccaccatca ctaagagcca gaagaactgc
     1801 aaaaggagtg cctgaagaat ctgactggca tcgtattttc caacaatgat cataactgga
     1861 gtataaatca aggacgaaaa aggcaaaaag gagagaatat ctgaaacaac ctttggaaag
     1921 aatgccaagg gaatcaaact ccccgacata aaagccacta tggaagtctt aagtaggttg
     1981 gaaccccaaa gatttttaaa cacaaaggct gaaaatccaa agcaaatatt aaagaaaaag
     2041 ttaatcagat aggcgagcgt taagctaaaa agataaagga cagttaatcc tagcacctct
     2101 acaatacctt gacccgatat gattttcatc aagacaatga cacttaaaaa tggaaggcca
     2161 acgctgataa aaatcaacca cttggaacca agctcggtga aaagatagga ggccgcaaaa
     2221 tgcactggtc gcaacaaacg catgataatg gagccatcct tgacctcctc cccaatcata
     2281 aaggacgaat cggatctagt cagaagattg gtcacaaaac tcatgatgat gtagagggtg
     2341 atatccgcca tactgaagcc ctgaatcaaa gactcttgcg aagaatcaaa gacagccttc
     2401 cagagataaa aggccacaaa agcccccatg acatcgccaa tccgatagag aataaagttg
     2461 actcggtaag taatcaactc ctgaacccct gcattgataa agggtttata acgtctccac
     2521 aatttgatca tcctagagct cctttcggta gaagcgacgg ataatatcct caatatccgt
     2581 atccaccatc ttcaaatcgc ggatttcaaa atcagatagg gtttgcttga taatgtcagc
     2641 tgactggtag cgagaactat caaattcaat gttgaggctg tttccttgtc tatcaatggt
     2701 catatcagac agaccttcat agtgagagac gagatgactt tgacctggta gcagttcaaa
     2761 agagagagtc ttcatcttac caaaggtctc cttgagttgg ctcaccgttc catcaaaaat
     2821 cttttgcccc ttgtcaatca tgaaaatccg atcacaaagt tgctcaatat cactcaaatc
     2881 gtgagtggtc aaaagaatgg tagtttcttc ctcttgattg atctgagtaa ttgcccgacg
     2941 aatattatcc ttaaccgaaa cgtccaaacc aatggtcggc tcatctaaaa aaagaacctt
     3001 gggattgtgg agcaaggagg ccgcaatatc cgctcgcatc cgttgtccca gtgaaagagt
     3061 ccgcacggga tccttgataa agtccttcaa atccaagact tcattcaaaa agtccatacg
     3121 cttatgaaag agcgagtttg gcacatcata aatctctttt aagacagtgt aggtctcttg
     3181 cagagccaaa tcccaccata gctgggtgcg ttgtccaaag actacgccaa tatctttgac
     3241 ataatcttgc cgattgtcct ggggaatctt gccgttaatc cgacaaaaac cagatgttgg
     3301 tttcaaaatt cctgtcagca ttttaatggt tgtcgacttc ccagcaccat ttgccccgat
     3361 aaatcctaaa atctgccctt ttggaacctc aaaggtcaaa tccttgaccg cttcaaaggt
     3421 ctgcttttca ggatgaataa aggagcgcaa agcccccttc aagcccggtt ccttaacaat
     3481 cttcacaaaa tgtttctgaa gatgttccac ttctatcatt gccatatcta tctccctatc
     3541 gtaaggaata gcaacattgt gtttttaact acaaatattc taaatcctta ctttctacac
     3601 cttctacatt ttattactac agaaggcttt ataccaacct attatagtcc aataaaaaac
     3661 gaaatgcaag cgtttgccta tagattgtga aattaggatt tctctcatga aataatactt
     3721 ttaatcaaaa attctgttta atagtttaaa ttaccaacct actcatcttc tcaccagagg
     3781 gcggtattaa ctttgatcat aaaatcggat actccctcta tccagaaaat atgctcagaa
     3841 tgattgtctc taaacaatac ctgctctttt acctcattca tgaaaaagaa gtccataccc
     3901 tgagaattat caactcacgc accgactacc taaaccaact cgaccacctc tttcgtacat
     3961 gtaggaaact at
//
LOCUS       DAVLWA010000118         3955 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLWA010000118 DAVLWA010000000
VERSION     DAVLWA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.118"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            190..642
                     /locus_tag="VYN80_001924"
     CDS             190..642
                     /locus_tag="VYN80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541778.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYICRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1461..1820
                     /locus_tag="VYN80_001925"
     CDS             1461..1820
                     /locus_tag="VYN80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541779.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1830..2195
                     /locus_tag="VYN80_001926"
     CDS             1830..2195
                     /locus_tag="VYN80_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1541780.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2182..>3955
                     /locus_tag="VYN80_001927"
     CDS             2182..>3955
                     /locus_tag="VYN80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1541781.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKIYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYE"
BASE COUNT         1431 a          600 c          788 g         1136 t
ORIGIN      
        1 gttaagtgaa aatattataa gtgagaaaga tggaagttat caacattcac tgactataat
       61 gaaggatttc aataatcaaa aaatagttaa ctatattgct catttcaata gttatgacat
      121 tttgaaaaaa ggttctcact tatcaggtgc atataaacga aaaatttttt ctaaagaaaa
      181 tacacgattt tggagtttaa tattaactcg aaagaatctt cgcaacaact tgatattctc
      241 tataaagaag ttggtgtatt aagtttggga caaaaagttg tagccatgct tgatttttta
      301 ttagcatata gtgattattc taaagacttc agaccattga ttattgatca gcctgaagac
      361 aatctagaca atcgttatat ttgcaggcat ttagttcagc agtttagaga tgtgaaagct
      421 caacgtcaaa ttattttagc aacacataat gctacaattg taacaaattc tatgacagat
      481 caagttgtta ttatggagtc agatggagtt aacggatgga ttgaatcaca gggatatgtt
      541 agtgaaaaat atataaaaaa tcatatcatc aatcaattag agggaggaaa agattccttc
      601 aagcataaaa tgtctatata tgagacggct ttatcagagt agagtcagaa aaagtaggtt
      661 agaaatttag cctacttttt tctttgtccg acaggcatag tgtgcatctg aggtccaagt
      721 cctctgtgga tatttgctgc agatgaaacc aatagcgact cctaagcctg aatatcgtga
      781 ggtagggggg ataggaagga attagcgaaa tcaaggttct acaaacagaa tcgtgacttg
      841 aagccatata tagcggatga ggaactctaa aatccaaata ggtgtcgtaa cctatatacg
      901 taaattacga gagtaaacta ggaaagatgt acggcttatt ccgtgagcgt ttaggacgta
      961 gtacaacgaa tcatgggagt cagctgaaca catagtattg aagaaatttc tgtaatggaa
     1021 atggagcgaa gaagtgaaca attaaatgaa tacctctcta attaaatttg tcaattctaa
     1081 ttcctggtat gaaaagacag tgacctgaaa atgtaaacga tgggagctga ttataaatat
     1141 aggacggtac atgcagtggt gttagagatt agtccctact tgatttgtga taacttcccc
     1201 aaatttcttc tgctatactt ttctcaactt ttaaaaatcc aactaagaat tttacctggg
     1261 ggtttggggg cgaagccacc aagttatctt atcgttggct gtcaaaactg gaaggttttg
     1321 gttagctggc gatatgattt tttgggtatt gtaaccacaa tgcttgagct cgcaaagacc
     1381 gaacaaggac tttaagaaat ttcccgtatg acacacgggg tcggggttta cccgagaaag
     1441 gatgaaagtg aggaaaagag atgtctgaac agtaccgtga cattcgaaaa gaggtaaatc
     1501 taaccgcaga tgagctaaaa cagattgaaa aaatgatgga ggttgataat tatcgtcact
     1561 tttcaccatt cgtcagggat aaaattttga tgactgatga caaacagtta gctgccaaag
     1621 aatggttttc cctttggcag tctcaaaagt ttgaacagat cagtcgagat gtgcatttgg
     1681 ttttgattat cgcaagagag aatcaccaag tgactcaaga acacgtgtca atcttattga
     1741 cctgcgttca agaattgatt gcggaagtca atcaagtaca gtcactcagt cgtgggtttc
     1801 gtgaaaaata catgagatag gaggctacta tggtttatcg ttatcgtacc aatctcaaaa
     1861 aagtatttct aacagattca gaattacacc aattgaatga acggattgct aagagcaatt
     1921 gtcaaaattt ctcagtctat gccagaaaag ttttgcttaa tcccaatatg tcatttgtca
     1981 cgattaacac tgatacttat gaacagttag tgtttgaatt gaggaggatt ggaaataaca
     2041 tcaatcaaat tgcgcgtgcg attaatcaaa gccatctgat ttctcagaac caattacaag
     2101 aattgagtaa aggagtcgga gagttaatta aggaagtgga caaagaattt caagtggagg
     2161 tgaaaagact gaaggagttt catggtagtt actaaacact ttgcgacgca cggaaaaaaa
     2221 tatcgtaggc gtttaattaa gtatattctc aaccctgata aaacggacaa tttgaaattg
     2281 gtatctgatt ttggcatgag caattacttg gacttcccta gtcatgcaga aatggtagag
     2341 atgtacaatg tcaacttcag caataatgac aagttgtacg aatccagaaa cgaccgacaa
     2401 gaaaaacacc aacaaactat tcatgctcat tacctcattc aatcattttc tcctgaagat
     2461 aatctgacac ctgaagaaat taaccgcatt ggttatgaga tcatgatgga attgacagga
     2521 ggtcgtttta agtttatcgt agcaactcac acagacaaag atcatgttca taatcacatc
     2581 ctaatcaacg ctattgaccg caattcagat aaaaagttga tatggaacta tgccttggaa
     2641 cgaaacctcc gtatgatttc agaccgtatt tctaaagtga ctggtgcaaa aattattgag
     2701 aaacgttatt cctatcgaga ttataaaaaa tatagggaat ctagtcataa gtttgaattg
     2761 aaacaacgtc tttatttttt gctacagcaa tctaaatcgt ttgatgattt tttagagaaa
     2821 gcaaagcagt tgcatgttca gattgatttt agtcaaaagc atagccgttt tttgatgaca
     2881 gatagaacga tgataaaacc aattcgagga cgccaactca gcaaacgaga tttatatgat
     2941 gaagactttt ttaaaatata ttttgccaaa caagagattg aaagtcgttt aaaattttta
     3001 ctaaagtctg tttattcatt agaagaattg catgtaaagg ctaaagagtt gaatctaacc
     3061 atcgagttaa aacagaaaaa tgtgatgttt accctagaag aagatggcaa aaagataagc
     3121 ctgagtcata aaaaaattag cgataagaaa ttgtacgatg ttcaattttt taatcgttat
     3181 tttgaagata gagaagttag agatattcaa gcattagaaa atcttcagga agattttcag
     3241 acttttcgag aggagcagca taaggagaaa gtctccgctg aagaaatcga ggaagccttc
     3301 aagaaataca aggaaaaacg agatgctatc catgagtttg agattgaact tacggataat
     3361 cagatagaga agttggttga tgatggtgtc tatatacagg tttcgtttgg tatcaaacag
     3421 agtggactca tctttattcc taactaccag ctagatattt ttgaggaaga caatcataaa
     3481 aaatataaag tctacattcg agaaacgtct tcttactttg tctataataa agaaaacatg
     3541 gataataatt gtttcatcaa gggtaggacc ttaatcagac aattgagcaa cgatagccaa
     3601 aaactcccct atagacgacc aactttaaaa agtcttaaag agaagatttc tgaaattaat
     3661 cttatgattg aactatctaa cacgaacaaa caataccaag aaatcaaaga tgagcttgta
     3721 ttagaaattg cagagattga tatgcagcta gaagaaaccc aagaaaaaat cgccacctta
     3781 aacaagatgg cggaagtcct tatcaatttg aagagtgaag atcatgagac tagaaaatta
     3841 gctaagtatg attttgacca aatgaacatg acggaatcaa taatgttaga tcgactcaat
     3901 acggatattt taaaattaca acaggaactt ggtaatgaaa ttaataaata tgagg
//
LOCUS       DAVLWA010000119         3916 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLWA010000119 DAVLWA010000000
VERSION     DAVLWA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.119"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            146..430
                     /locus_tag="VYN80_001928"
     CDS             146..430
                     /locus_tag="VYN80_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541782.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            423..671
                     /locus_tag="VYN80_001929"
     CDS             423..671
                     /locus_tag="VYN80_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541783.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            767..985
                     /locus_tag="VYN80_001930"
     CDS             767..985
                     /locus_tag="VYN80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541784.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(1353..3677)
                     /gene="pflB"
                     /locus_tag="VYN80_001931"
     CDS             complement(1353..3677)
                     /gene="pflB"
                     /locus_tag="VYN80_001931"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1541785.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1209 a          713 c          758 g         1236 t
ORIGIN      
        1 agatgaaaaa cagaattttt cgattgtgat ttaaagcaac aagaagattt tcagtatcat
       61 cctatagata cgagctaatt aagaaaaact acatttttga atataaacta caataatata
      121 aactaaattt tataggagga agacaatgga ttggtacgat tatatgatac aggcatccaa
      181 acaatcacaa ttcaacgcaa gccattggtt tcgctatttg cgaaaagtta tttttgaaga
      241 ctattcttat ttaacaaacc aagatgtaga aaagttgcta gactccaaag aactaacccg
      301 ttttcaaaaa attagcttga agtatgcctt tcaagagcat actccaactc ataaatatgt
      361 gatttcatta aataaacctg ctaagttaac caatgttcaa aaattgatgg agaaatacaa
      421 acatggataa aatgaaaccg gtcttccaag ccctaaataa ggaattaatt caggaaaatc
      481 tgactttaac aattatctgt gtcggtggtt atgtcttaga atatcatggt ttacgtgcca
      541 cacaagatgt tgatgctttt tatgaccaaa atcagaaaat caatgaaatt attgctcgtg
      601 taggaaaaca gtttaatctt aacatccacg aagagttatg gctcaacaat catgttgcaa
      661 agcaaatatg aacaaacaac ctcccttaag tttatgcgaa tccttgtact catttgagaa
      721 tctgaccgtt ttagtagtcc ctattgagta tgtgttagga atgaagatga tgagtatcag
      781 agagcaggat ttgcaagata ttggtgcaat tattaaatac aaaaactttc attcaccatt
      841 cgataccttt aaatatttaa aagacatggg atttgatact attgacctct ctgttctatt
      901 agaaggtttt agttatgctt atggtatgga ttggttagaa aaatttttta aagaaaatca
      961 agataaactt cgcgaatttt attaacagta tcaaacaaga tgattgtcaa ttagccagtc
     1021 gtcttgtttt ttatgatgaa atgaaataaa atctgaacaa atcgcttaga gaattccagt
     1081 caatttctag cgctgtttga gacgtttcag tggactatag taaaatgaaa taagaatagg
     1141 acaaatcgat caggacagtc aaatcgattt ctaagaatgt tttagaaata aaggtgtact
     1201 attctagttt caatctatta tatttcagat tcggtccact attccttccc aaaaacattg
     1261 catattgcgt caaaggaatt gctcacacta tcccaagcac aaaaccctca gtcgattgac
     1321 cgagggttcc tttttattat tcaagaactt gattagctca atgcatccaa ggcgtcatcc
     1381 attgaaagaa cttcgtggaa gacacgttgt gtcaattcag ttttttgttc tggagtgagg
     1441 tatttagtgt ttacacagta tccagagata cgtacgataa cgtcttcgcc tgacatgatc
     1501 ttttcgtaaa catcgttcaa gtccataacg ttcaagttaa cgtgttgtcc accgttttcg
     1561 aagtaaccat caaggattgt taccaagtta tcaacttgtt catcacgagt cttaccaaga
     1621 gcgcgaggtg atacttgtgt agtcaatgag ataccgtcag ctgcataact aaagtcaagg
     1681 ctagaaagtg agttcaagtt ttgcaaccaa ccacctttag ctttgttaga tgggttagca
     1741 cctggtgaga agaattcaag tttagacaag ttcacagaac catcttcgtt gaggtataca
     1801 cctttgtgaa ctggtgagtt accagtttgt ttagagtaag caacgttaga tgtgattgtc
     1861 aaaagtgata ctgtagcttc tgcgtctttg tatagtttgt ggctacgtag acgagttgtg
     1921 taagcttcga tcaaccattc tgccaattcg tttgaacgtg ggtcatcttc accccagcgt
     1981 gggtagtcac cgattgtttc gtaatcgtag atgtagccat cttcgtcacg gattggttta
     2041 actgtagcgt atttgatagc tgacaatgta tcaacagtgt tagcaaatcc acagataccg
     2101 aatcccatgt tggcacgttg tttagttggc aagaaggcca tttgaacagc ttcgtagttg
     2161 tacctatcag tcatgtagtg gatgatgttc aaggcatcta cgtaagtgtc agtcaaccag
     2221 tcaagagatt tttcaaagtt cgctttaact gattcgaatt caagaacttc gtcacggatt
     2281 ggttcgatat caaatacttt gtagtctttg tgaacatcgt cgtaaccacc attcaaacca
     2341 gtaagaaggg ctttaagaac gtttacacga gcaccgaagt actggatgtt gtggcgttgt
     2401 tcttcatttt ctggatcaag tggagacaca cagcatgaga tacagctcat ttcaccatat
     2461 ccgtctttag ccattgttgt tacaccttcg tattggatag aagagtgttt gtggctcatg
     2521 tgcatacagt agcgacggaa gttgtatggc aatttgtcag tccaaagaac tgtcaagttt
     2581 ggttctggtg agttaccgat gttgtcaaga gtgttcaaga aacggtagtc catcttagta
     2641 acacggtgac gaccgtcgtt acccatacca gccatagaag ttgtgataaa ggttgggtca
     2701 cctgagtaca attggtcata agcttttgta cgagcaaatt taactgtacg aagtttcata
     2761 acgaaatcat caacgaattc ttggatttct gattcagtaa atgtaccacg agcaaggtca
     2821 cgttctgcaa agatgtccaa tacgattggt acacgaccta gagatgtagc agcaccgtta
     2881 atcacacggc agacagccat gaaagcaatg ttaacccatt ggattgcttc tttcacgttc
     2941 atcgctggtt tgcgaacatc aaccccgtaa aggtcaccca ggcgaacaac ttgttgcaat
     3001 gcttggtatt gaaggtttac ttcttcacga agacggattg tttcttcatc gatttctttg
     3061 attgcattcc agtcgtttac tttttcttgc atcaagtagt ctgcaccgta aagagcaaga
     3121 cgtgcgtaaa caccgatgat acgtccgcgt gagtatgcat ctggaagacc agttacagtg
     3181 tgtgcatgac gagcgcgacg aatatttgaa gtgtaggcac ggaaaatacc gtcgttaact
     3241 gttgttacat atttagtgaa gatttcgtga acagctgggt ctggttcgta tccattttct
     3301 tttaaagtag tttcagccat acggatacca ccttttggca tgaagttcaa tttgaagagt
     3361 tcatcatttt ggataccaaa gataacttca ttttctttgt cgataaatcc agcagggata
     3421 tcagcgatag atgttggacg agtgtccatt gggaaacgag tttcttcgta gtgtgcttta
     3481 gtttcttcta caattttctt gatgtgaagt gaacgctctg ttggtcctgc aaggaagctt
     3541 tcgtctccat cataaggtgt gtagttagct tgtacaaagc gtgatacact tgctttttct
     3601 ttccaatcta cgcctttgaa gccttcccaa gctttgtcaa aaatatcttg tgcttcaaca
     3661 actgtcttaa caaccatatt aatatcctct tttttctagt aacagtcatc tgttacattc
     3721 ataagacaag tataccataa agtaaccgat ttcaacaagt gcaaaatccc tatttttaca
     3781 ctttcttttc taaaacagtc tatattttat ttcaaactgt attatatttt tgaaaaaaat
     3841 aaagtccttt tttctttttt tcagaaaaaa gggtataata aaagaaaata agcagtaaca
     3901 ctcaatggaa atcgaa
//
LOCUS       DAVLWA010000120         3832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLWA010000120 DAVLWA010000000
VERSION     DAVLWA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.120"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..>3832
                     /locus_tag="VYN80_001932"
     CDS             <1..>3832
                     /locus_tag="VYN80_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1541786.1"
                     /translation="GEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQETAGVH
                     YKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLVELPNTGSKNEMQA
                     LVAGASLATLGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLLNYNTD
                     YELTSGEKLPLPKEISGYTYIGYIKEENITSESEVSNQKSSIVTPTKQQKVDYSVTPN
                     FVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQLAEHKN
                     LETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEIQENPD
                     LAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTKKAQVI
                     KEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKGVPEVQ
                     PALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPEQVAPL
                     PEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVE
                     PSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPSAEDTK
                     PNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNS
                     TDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPET
                     TGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQYQGGN
                     PGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYR
                     HKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENYFMKVK
                     SENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKTQQSET
                     GVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGTNNEKY
                     YAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHADGAIA
                     GERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLIDKSFAS
                     IDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLWDPVGG
                     LEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEILTKDS
                     TVQRGEVLEDAQIKE"
BASE COUNT         1453 a          582 c          797 g         1000 t
ORIGIN      
        1 ggtgaaaaac aagagcgttt ttcatttaga aaattatcag taggacttgt atctgcaacg
       61 atttcaagtt tattttttat gtctgtatta gctagttcat ctgtggatgc tcaagaaact
      121 gcgggagttc actataaata tgtggcagat tcagagctat catcagaaga aaagaagcag
      181 cttgtctatg atattccgac atacgtggag aatgatgatg aaacttatta tcttgtttat
      241 aagttaaatt ctcaaaatca actggtggaa ttgccaaata ctggaagcaa gaatgagatg
      301 caagccctag ttgctggtgc tagcttagct actctgggaa ttttaatttt tgccgtctcc
      361 aagaaaaagg ttaagaataa aacggtatta catttagtat tggttgcagg gataggaaat
      421 ggtgtcttag tttcagtcca tgctttagaa aatcatcttt tgctaaatta caatacggac
      481 tatgaattga cctctggaga aaaattacct cttcctaaag agatttcagg ttacacttat
      541 attggatata tcaaagagga aaacataact tctgagtctg aagtaagtaa tcaaaagagt
      601 tcaattgtca ctcctacaaa acaacaaaag gtggattata gtgttacacc gaattttgta
      661 gaccatccat caacagtaca agctattcag gaacaaacac ctgtttcttc aactaagccg
      721 acagaagttc aagtagttga aaaacctttc tctactgaat taatcaatcc aagaaaagaa
      781 gagaaacaat cttcagattc tcaagaacaa ttagccgaac ataagaatct agaaacgaag
      841 aaagaggaga agatttctcc aaaagaaaag actggggtaa atacattaaa tccacaggat
      901 gaagtgctat caggtcagtt gaacaaaccg gaactcttat atcgtgacga aactatagaa
      961 acaaaaatag attttcaaga agaaattcaa gaaaatcctg atttagcaga aggaactgta
     1021 agagtaaaac aagaaggtgc attaggtaag aaagttgaaa tcgtcagaat attttctgta
     1081 aacaatgaag aagtttcacg agaaattatt tcaacttcaa cgactgcgcc tgtttcaaga
     1141 atagtcgaaa aaggtactaa aaaagctcaa gttataaagg aacaagctga gactggtgca
     1201 gaacataagg aagtacagtc tggagctatt gttgaacccg caattcagcc tgaactacca
     1261 gcagctgtat taaccgacaa aggtgagtca gcagtccaac cagagttgcc agaagctgta
     1321 gtaagtgata aaggcgtacc agaagttcaa ccagcgttac cagaggcagt catgactgac
     1381 aagggtgatc cagagcaggt agaaccgcta ccagaatata caggagtaca agctggagct
     1441 attgttgaac ctgaaaaagt tgaaccggaa tatgctggag tacaagctgg cgctattgtt
     1501 gaaccagagc aggtagcacc actaccagaa tacacagggg tgcaagccgg agcgatagtc
     1561 gagccagaga aagtagaagc accgaaggaa tacacaggag tgcaggctgg agcgatagtc
     1621 gaaccagaga aggtagaagc accgaaggaa tacacaggag tgcaagccgg agcaatagta
     1681 gaaccagaaa aagtagaacc atcaaaagaa tacacagggg tgcaggctgg cgctattgtt
     1741 gaaccagagc agatagcacc actaccagaa tacacagggg tgcaagccgg agcgatagtc
     1801 gagccagaga aagtagaagc actgaaagaa tatactggta aaatcgaaca acctagtgca
     1861 gaagatacta aaccaaataa tgaaaatact aatacaccag aagaaatgag tattcagaaa
     1921 aaatctagtg cattaattaa tatgaacttt attacagatt cgtcgaaggt tactggagta
     1981 ggatcagcta cttttattgc accaaatgta cttttaacag tagcacataa tttcattaat
     2041 aattctactg ataatactac tggcgaattt agaggtgata aatctaaaaa cgtatatgag
     2101 tgggtgactc ctgatggaca aaaaggaaca tttactgcta acaatattca tttttataat
     2161 aaaaaagatt atccaaaagg atttatctat gatttagcag tgattaagct acctgaaaca
     2221 actggcagag aacatgtaga attagttaaa aattattcga aagtaaatct taatgacaaa
     2281 ctaaatgttc atggatatcc agctgggaaa tatacgcact taaaagatgc tacggtagaa
     2341 atggaacaag aatatgcgaa taacacctac ggtgttcaat atcaaggtgg aaatcctggt
     2401 atgagtggtg gaggaatatt taatgctaat ggtgaagtta ttggtgtgca tcaaaatgga
     2461 gcacaaaatc gctcaggcgg attgatttta tctcctacac aattagcttg gattaagagt
     2521 attatagcag gtaatgaaat tccaccagta tacgatgaac tttatcgtca taaagatgag
     2581 aaaaaagatg acgctaaaga tgaaaaagaa gttattaaga aattagaact tagaaatata
     2641 tcttctgtag aactatattc aaaagatggt aataagtatc gacatgtaac ttcacttgct
     2701 tctttaccaa gtaatgcaga aaattacttt atgaaagtta aatcagagaa ctttaaagat
     2761 gtgatgctac cagttacaag cattactaat gatactaaag ataatagaga tgtttataag
     2821 atagtagcaa gtgcaaatag tctgatacaa catgaaaata ataatgtact agaaaattat
     2881 acatactatt taccaaaaac tcagcaaagt gaaactgggg tatatacatc atttaaaaat
     2941 ttagtagatg caatgaatag caatcctaat ggcacattcc gtttaggagc aactatggat
     3001 gctcgtgaag ttgaacttcc agatggtcaa gaaagttatg tgaataatgt attccatgga
     3061 atattagtag gaacaaataa tgagaaatat tatgctattt ataatttgaa aaaaccatta
     3121 tttggggaat taaatggtgc tacagtagaa aaactttcac taaaagatgt taatatttcc
     3181 gcaaaggacg atactgcaac acttgcgaaa gaagcaaata acaatactca tattgataac
     3241 gttcatgctg atggagcgat agctggggaa cgtagcatag gaggattagt atcacaagtt
     3301 aataattcta ctatttctaa cagtagctat acaggtagaa taactaatac atataaaaca
     3361 gttgctagtt atcaaatcgg aggtttagta ggcaaacttt ctggtccgag gggattaata
     3421 gataagtcgt tcgcatcaat cgatttatct tcaaatgcaa cacaaggaga ccagtctatt
     3481 ggtggaatag ttggtgcagt agaaaatagt gcgcttatta gtaatagtta tgctgaagga
     3541 aatctaaata atgttcaacg ttttgcaaac gttggtggag tagttggtaa tctttgggat
     3601 ccggttggag gattagaaaa atccggtcga ctttctaatg tactaagcga tgttaatgta
     3661 acgaatggta atgctattgc aggttataat tttaatggta taaaagcaaa tggcacatac
     3721 agtaataaaa ataataaggt tgtgaacgta gtacaagaag atgatgaaat cttaactaag
     3781 gattctactg ttcaaagagg agaagtatta gaagatgcac aaataaaaga ga
//
LOCUS       DAVLWA010000121         3818 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLWA010000121 DAVLWA010000000
VERSION     DAVLWA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.121"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1070
                     /locus_tag="VYN80_001933"
     CDS             <1..1070
                     /locus_tag="VYN80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV1541787.1"
                     /translation="IKKNKILFINFNGKGYGDNPKSICEYLRVTYPELDLVWLTKDNE
                     DFPDGVRVVRYKSLQSFYEQASSKVWVYNVRNFERLLKKRGQFYIQTWHGASSFKLIE
                     KQADLPLKYILEAKYDARVTDIMISDSRKQTEEFQKYFWYSGEIFEVGMPRNDALFHY
                     KEDYDKLNNIRKKLSIDSDDYVILYAPTFRDDGDASYLDINFERLLQSIEQEIKKKCK
                     ILIRLHPNDSHFSNNISFNHDIIDVTLFSDMQELILLADVLLTDYSSAIFDFMLLNKP
                     YVRYVNDLEKYAELRGVSDTYYELPDSIIKTAEELYDLLPKKIENFDYDSIKKYRNEI
                     LCPIFNGTASENVGRRIIQEL"
     gene            1106..2134
                     /locus_tag="VYN80_001934"
     CDS             1106..2134
                     /locus_tag="VYN80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1541788.1"
                     /translation="MKNNDLKIGSGAIHQISATLSQNNISGKILYCADPVVDDLYGSI
                     VRSQIEEIGRVKEESCNYNTIAYAMNIAERAIATDIDCIVGMGGGRVLDVCKYASFIS
                     KRPYLSIPTTAANDGIASPVAVLKRQDDRPKSLGAAIPSMTLIDIDVIASGPIQNIKA
                     GIGDTISNYTALKDWELAVERGKDEMHGFAYLMSQNSLDALMKTKYNSITPDFIEVLV
                     NSLVLSGIAMDFAGSSRPVSGSEHLFSHALDYYGSTRNLHGIQVALGTVAVLKLIENS
                     VDTVVDYLQRFEVHINPKLLGIDEELFIYCMQHATKMRSNRYTYLHEVDLSTDRLKQI
                     YKELISEL"
     gene            2150..2854
                     /locus_tag="VYN80_001935"
     CDS             2150..2854
                     /locus_tag="VYN80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEV1541789.1"
                     /translation="MKALILAAGLGTRLAPITNEVPKSLVPVNGKPILMKQIENLYQN
                     NITDITIIAGYKSSVLTDAVTEKYPEINIIDNVDFKTTNNMYSAYLGKAAMGDSDFLM
                     MNADVFYDASVIKSLLLHKAPNAIVTDLGIYIEESMKVVEKNGRLVEISKQISPEETL
                     GASIDVYKFSYEAGARFFEKCKEFIEDKRELQMWSEVALNAILSEVEFVACPLEGRWL
                     EIDNHEDLVAAEKLFA"
     gene            2869..3702
                     /locus_tag="VYN80_001936"
     CDS             2869..3702
                     /locus_tag="VYN80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV1541790.1"
                     /translation="MKLTNRVDYFGADISELQNKKLFLFDMDGTIYEEDRLFEGTLEL
                     LDYIHNIGGEYIFITNNSSKSVVDYVEKVNRLGIKAERDNFFTSAQATIVYIKENYPK
                     SKVYCQGTKSLIKELSDAGIDVTEQVSADIDVVLVGFDTELTSDKIRNTCEILSTKDV
                     PFIATNPDIRCPVSFGFIPDCGSICDMISKSVDRKPVYIGKPEPTMVDIVRKKLNYSL
                     FETVVIGDRLYTDIMTGINAGVTSVCVLTGEATVNDIQQGSIKPTYTFKNVKEMWKGI
                     V"
BASE COUNT         1312 a          485 c          760 g         1261 t
ORIGIN      
        1 ggataaagaa gaataagata ctatttatta attttaatgg taagggatat ggtgataatc
       61 cgaaaagtat ttgtgagtat cttcgagtta catatccaga gttagattta gtttggctta
      121 caaaagataa tgaagatttt ccagatggag tgagagttgt aagatataaa tctctacaat
      181 ctttttatga gcaagctagt tcgaaggttt gggtttataa tgtaagaaat tttgaaagac
      241 tactaaaaaa acgcggacag ttttatattc aaacttggca cggtgcttct agttttaaac
      301 tgatagaaaa gcaggctgat ttaccattaa aatatatttt agaagcaaag tatgatgcaa
      361 gagtcacaga tataatgatt tcagatagca gaaaacaaac tgaagaattt caaaagtatt
      421 tttggtattc aggagaaatt tttgaggtgg ggatgccgag aaatgatgcc ttgtttcact
      481 ataaggaaga ctacgacaag ttaaataata tcagaaaaaa actaagcatt gattcagatg
      541 attatgttat tttatatgcc cctactttca gagatgatgg ggatgcatct tatttagata
      601 taaattttga acggcttttg caaagtattg aacaagaaat taagaagaaa tgtaaaattt
      661 taattagatt acatcctaat gactctcatt ttagtaataa tatttctttt aatcatgaca
      721 taattgatgt tacgctattc tcagatatgc aggaacttat attattggca gatgttttac
      781 tgacagatta ttcttccgcg atatttgatt ttatgctttt aaacaagcct tatgtcagat
      841 atgtgaatga tttagaaaaa tatgctgagc ttaggggggt atcagataca tattatgaat
      901 tgccggattc tattattaaa actgctgaag aattgtatga cttactgcct aaaaaaatag
      961 aaaattttga ttatgattct ataaaaaaat atagaaatga aatactgtgt ccaatcttta
     1021 atggaaccgc tagtgaaaat gtaggaagga gaataattca agagttgtga agtaaaacat
     1081 tgggttattt ttaaggtaac gagatttgaa aaataatgat ttaaagatag gaagtggagc
     1141 tattcatcaa atctccgcta cactttctca aaataatatt tcgggaaaga ttttatattg
     1201 tgctgatcca gttgtcgatg acttgtacgg ttcgatagta cgttcacaaa tagaggaaat
     1261 tggtcgggta aaggaagaaa gttgcaatta taatactatt gcttatgcga tgaatatagc
     1321 agaaagggct attgccacag atattgactg tattgtggga atgggaggag gtcgtgtttt
     1381 agatgtctgc aagtatgcat cttttatttc gaaacgtcct tacctatcga ttccgacaac
     1441 agcagcaaat gatggcattg cttccccagt tgctgttttg aaaaggcagg atgatagacc
     1501 aaaaagctta ggggcggcta tcccctcaat gacactaatt gatattgatg ttattgcatc
     1561 aggacctata caaaatataa aagctggtat cggtgataca atatccaatt acactgcatt
     1621 gaaagattgg gagttggcag ttgagcgagg gaaagatgag atgcatggtt ttgcatatct
     1681 aatgtcgcaa aattctttag atgctttaat gaaaacgaag tataattcta ttacccctga
     1741 ttttattgaa gttttggtaa actctttagt tttatcaggc attgcaatgg attttgcggg
     1801 aagtagtaga cctgtcagtg ggtcagagca cttatttagt catgcattag attactatgg
     1861 ttctacaagg aatcttcatg gaattcaggt tgcattaggt acagtcgcag ttttgaaatt
     1921 aattgaaaat tctgttgata ctgtagtgga ttacttgcaa agatttgagg ttcatattaa
     1981 cccgaaactt ttggggatag atgaagagtt gtttatttat tgtatgcaac atgctacaaa
     2041 aatgagaagt aatcgctata cctatctgca tgaagttgat cttagcacag atagattgaa
     2101 acaaatatat aaggaactca taagcgagtt gtagaaaaga gaggaaaaaa tgaaagcact
     2161 tattttagca gcagggttag gaacacgtct agctccaatt accaatgagg taccaaaatc
     2221 tttggtacca gtcaatggta agccaatttt gatgaaacaa attgaaaatt tatatcaaaa
     2281 taatattaca gatattacga ttattgctgg atataagtca tctgtattaa cagatgcagt
     2341 tactgaaaag tatccagaaa ttaatattat tgataatgtt gattttaaaa cgactaataa
     2401 tatgtattca gcctatctag gaaaagctgc aatgggtgat agtgacttct taatgatgaa
     2461 tgcagatgta ttttatgatg cttctgttat taaaagtctg ttgcttcata aagctccaaa
     2521 tgcaattgta actgatttag gtatttatat tgaagagtct atgaaagtcg tagaaaaaaa
     2581 tggtcgttta gtagaaattt ctaaacagat ttcacctgag gaaactttag gagcttctat
     2641 tgatgtttat aaattctctt atgaagcagg tgctcgattc tttgaaaagt gtaaggaatt
     2701 tattgaagat aaacgagaac ttcaaatgtg gagtgaggtt gctcttaatg caattctttc
     2761 tgaagttgaa tttgtagcat gtccattaga gggtcgttgg ttagaaattg ataatcatga
     2821 agacttagtt gctgcagaaa aactatttgc ttgaaccgaa tacggagaat gaaattgaca
     2881 aatagagttg attattttgg tgctgatatt agtgaacttc agaataaaaa attattctta
     2941 tttgatatgg atggtaccat ttatgaagaa gataggttgt ttgagggtac tctcgaatta
     3001 ttagactata ttcataatat tggcggtgag tatattttta ttacaaacaa ttcatctaag
     3061 tctgttgttg actatgttga aaaagttaac agattaggta ttaaagctga acgagataat
     3121 ttttttacct ctgctcaagc cacaattgtt tatattaaag aaaattatcc taaatctaaa
     3181 gtttattgcc aaggaacaaa atctttgata aaagaactat ctgacgcagg aattgatgta
     3241 actgagcaag ttagtgctga tatagatgtt gttcttgttg gttttgatac agaattaacc
     3301 agtgataaaa ttcgtaatac ctgcgagatt ctatcaacaa aggacgtacc tttcatagct
     3361 actaaccctg atattcgctg cccagtatcg tttggattca tcccagattg tggttctatt
     3421 tgtgatatga ttagtaaatc agtcgatagg aaacctgttt acataggtaa acctgaacct
     3481 acgatggttg atattgttcg aaaaaaatta aattattctc tatttgaaac agttgtgatt
     3541 ggagatcgct tgtatacgga tatcatgact ggtataaatg caggagtaac ttcagtttgt
     3601 gtgctgacag gagaagcaac ggtgaatgat attcaacaag gtagtataaa accgacttat
     3661 acatttaaaa acgtgaaaga aatgtggaaa ggaattgtct gattttttca ttctgaatag
     3721 tgaattcatg aatactgtca aaatactcgt atgtatttaa attgatttta ttattagact
     3781 tcctgcaaaa ctagaatcct agttcatgat tgataata
//
LOCUS       DAVLWA010000122         3656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLWA010000122 DAVLWA010000000
VERSION     DAVLWA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.122"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(163..1578)
                     /locus_tag="VYN80_001937"
     CDS             complement(163..1578)
                     /locus_tag="VYN80_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1541791.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1591..3429)
                     /locus_tag="VYN80_001938"
     CDS             complement(1591..3429)
                     /locus_tag="VYN80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1541792.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3447..>3656)
                     /locus_tag="VYN80_001939"
     CDS             complement(3447..>3656)
                     /locus_tag="VYN80_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1541793.1"
                     /translation="FAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTE
                     YNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1227 a          747 c          484 g         1198 t
ORIGIN      
        1 ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta atccccctat
       61 aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata tttaatgaga
      121 tgctgcctta accgtgataa tatctaatca tcaataaaca aactatttga ataaggattc
      181 tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat atcgattgaa
      241 tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct tagaaagctg
      301 tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca taatttccac
      361 accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact gaattctata
      421 atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt tagcacctaa
      481 tccgttttcc actataaata atggaatttg ataacggtca taatatctat ttaaaattat
      541 acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat aaggatttac
      601 taaaccacca ataatattcc cttctcctga attatactgt gttggaagag cagattgagt
      661 cacactcatg taatagctaa aggataaaaa atctacagta taatttttta ataactctgc
      721 atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt ttgcataatt
      781 cggataataa cctctaacat gcacatctga aaatagataa tttagattct catactcatg
      841 agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag ctaataccat
      901 acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca aactcgaggc
      961 gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct ccttaggtat
     1021 atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt taaatgtaag
     1081 ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt tttcataaaa
     1141 atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata atggagtctc
     1201 atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat caaacaattc
     1261 atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg gaaaaattct
     1321 actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca aggatatatc
     1381 ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt ctgtaggatt
     1441 ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata agcccttacc
     1501 atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc aaagaaaatc
     1561 atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt ccttagaatg
     1621 ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg tattcgttac
     1681 aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt ccatttcaaa
     1741 aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa aaccttgacc
     1801 ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct catcactctt
     1861 caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa atggtgcata
     1921 aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat ctgaaaatgt
     1981 tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa atatcatttt
     2041 tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt catcgattcc
     2101 aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa ttaaagcatt
     2161 tacaatattt gatggcacat cagaataaat aaattgaggc aacgctatca aagatgggac
     2221 agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta atccaccacc
     2281 aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata atgcaggttc
     2341 ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt
     2401 atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt ttgaccctaa
     2461 cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa aaataggtgc
     2521 aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa gaatagctaa
     2581 tgtaagccat ccagctacac catacatttg cccaactaga tttgataatc cttcaccaac
     2641 aattactcca ataggcccga ctacaactaa ggcaatacag cttgatacta ataatactag
     2701 cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca ttttttcaat
     2761 atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat aactagctgg
     2821 tgtcacaggt acaccaaata aactaagagg attccctgat tgcaccattt gaacaaaatt
     2881 tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt ttaatttttg
     2941 tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc caaaaaatgt
     3001 caaaaaagca atagtctgag aatctgattg caatatacca agcattggta aaataattac
     3061 caagactttc agcatacctc cccctaacat tgctggaatg attggagtca tggaaccagc
     3121 gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa ctgaatcgga
     3181 ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat taccaagtat
     3241 aatttgatat tgtccattct ttttcataat acctattaca cctggtatct tcttcacatc
     3301 atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta cacaatgggt
     3361 aactctattg acattttttt cacctccaat tacatcgagg attttttgta ccgtatcttt
     3421 ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg acgaatatga
     3481 gccatcaaat aaactaattc actagaagtc agcaaataat tgtactccgt ttgtataaac
     3541 attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat taatgctaac
     3601 aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg ggcaaa
//
LOCUS       DAVLWA010000123         3565 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLWA010000123 DAVLWA010000000
VERSION     DAVLWA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.123"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..78)
                     /locus_tag="VYN80_001940"
     CDS             complement(<1..78)
                     /locus_tag="VYN80_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541794.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASH"
     gene            complement(264..875)
                     /gene="rpsD"
                     /locus_tag="VYN80_001941"
     CDS             complement(264..875)
                     /gene="rpsD"
                     /locus_tag="VYN80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1541795.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1070..2122)
                     /locus_tag="VYN80_001942"
     CDS             complement(1070..2122)
                     /locus_tag="VYN80_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1541796.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2119..2817)
                     /locus_tag="VYN80_001943"
     CDS             complement(2119..2817)
                     /locus_tag="VYN80_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1541797.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2984..>3565)
                     /locus_tag="VYN80_001944"
     CDS             complement(2984..>3565)
                     /locus_tag="VYN80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1541798.1"
                     /translation="WSDNGDAKNPALSPLGENVKTKGQYFYQLALDGNVAGKEKQALI
                     DQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNS
                     GSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNT
                     GETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          980 a          796 c          689 g         1100 t
ORIGIN      
        1 gtgtgatgct tctgttatac tacctgttcg ctcacaataa gagagaactt ttttacgaaa
       61 atctattgaa tatgccataa aaggattata ccacattgtg tactattttt ggttcatttt
      121 actatatcac tttggactat atcggtctag ttatgtctca acgattatag ctggatacta
      181 agagaaatca aaaaagcact aagggagaac gtcccaaagt gcttaatatc aaggcctcaa
      241 agtctatctt attcaataaa atattacaac attttgttgt agaattcaac gacaagtgct
      301 tcgttgattt ctgggttgat ttcgtcgcgt tctggcaagc gagtcaatga accttccaac
      361 ttttcagcgt cgaatgatac gaatgctgga cgtccaagag tagcttctac tgcttcaagg
      421 attgctggaa ctttcaatga tttttcacga actgagatca cttgacctgg agttacacgg
      481 tatgatggga tatcaacgcg tttcccgtca acaaggatgt gaccgtggtt tacgaattga
      541 cgagcttgac gacgagtagt cgcgagacca agacggtaaa caacgttatc caaacgacgt
      601 tccaaaagaa gcataaagtt gaaacctagg attccgcctt tgatttttgt agcttgtacg
      661 aacaagttac ggaattgttt ttcacctaca ccgtaagtga aacgaagttt ttgtttttca
      721 gccaattgca aaccgtattc tgacaattta gaacggttgt ttggtccgtg ttgtcctggt
      781 acgtagttac gacgtgccaa ttctttacct gtacctgtaa gtgaaaggcc aaggcgacga
      841 gcttgtttcc aagatggtcc tgtataacgt gacatatgaa tgtcctcctg atataaataa
      901 tatttcggtg gaaatagtca cttagaaagc cctgattcgt gcagatgccc ttcgcctaaa
      961 cagccaaggt tacttgtcat aagacacctg ttgacgagct tcatgctttc ctgctgatat
     1021 ttcacacaaa atctattcta ccatgaatag ctggatttgt aaaggggttt taggctttat
     1081 tttcactacc agagaggttg agaacgaggg taaaggtact tcctagaccg tactggctgc
     1141 tgactgtgat ttccccaccc aattgatggg ccaattcacg cgcaatcgca agtcctaatc
     1201 catgaccacc tgtcttcatg ttacgcgaag tttcgacacg ataaagacgt ttgaaaatat
     1261 tttccaaatc ctctggggca ataccctgcc cttcatcggt cacactgatt gaaagctggt
     1321 ccttctccag cttagccacc acttccagct tggttcctgg agcagaatat ttaaaagcgt
     1381 tatcgaccag attcaccaag atacgagaaa gcttagcata atctccctca atccgggcag
     1441 actctgggat tacctgcaag tggacatctc ttctctcctg ctcaatcaaa aactgaaatt
     1501 cactcatgca ctcaattaag agcttgtcca gaaaaatact gtctttactg gtagtttcca
     1561 cctgattcct agctgtgttt agggtcaaaa aattcaactc ctcaaccagt ttattgagcc
     1621 tctccgtctg gcgtccaatg gttgctagat aatgagcttg ctccgactcc ttgataatcc
     1681 catccaaaat cccttctacc gtcgcttgga tcgaagtgat aggagtctta atatcatgcg
     1741 acaactgggc aatcatcaag cccttttctc gttcgctttc ttccaaggaa tcaaagctta
     1801 cctgcaaatc atgggacatc tcattaaaag tttgccctaa ttgctgaaat tctacaggac
     1861 cttgaacctc caaatttgaa ggaaaatcct tggccgctac ccgcttggca tgctccttga
     1921 gtttgcccaa cgacgtaaag actggcaata ggagaaagag actaatccca gcaccgacaa
     1981 ggctggcaac gatggtcatc ccaagcaaaa agtaaatctc gcctttcgca atcagcattt
     2041 tttgaacagc ccaaaaaaca accaaaatgg ttaagagggt tgaaataata tatccaacca
     2101 aaatataact ttttagtttc atgtttgtcc tctcggtttc tctatcttat atcccaaccc
     2161 ccaaactgtc ttaatagtgg gcgtttggtc actactatat tttgccagct cctgtcgaag
     2221 agcatggata tgcacattca aggtattggt gtcatccacg tagtcttctt tccagatctt
     2281 ttcatagagg tctgtcttgg agaaaactcg ctctggattg ctagctaaaa tccacagcaa
     2341 ttcaaatgat ttaacagtta aatccagcat ttcttctcct atttgaactt catgactact
     2401 atgattcatt tttagattgc caagggaaat cagctctgtt tcgcccccac gatgaaggcg
     2461 gcgcaaaata ttgtggacac gcaaaaccag ctcacgtggg ctaaaaggct tagcaataaa
     2521 atcatctgct cccaagctca ggccgtaaat cttgtcctgt tcactggtct tagcagtaat
     2581 aaataggaaa ggctgctctg gtgataagta ttgaacctca ctgattaaat cataaccatc
     2641 catccgaggc atcatgacat ctgtgataat caaatcaatc ggttttttct tgaacagctc
     2701 tagcgcttcc agtccatcat gggctaccaa gacctgataa cctgcctgaa ttaagtatct
     2761 ctgatgaata tctgtgattt ctacctcgtc gtcaacgagt aaaattgtct ttcccatctg
     2821 tctctccttt gataaaaaca gtgctatact gctttaagta taacactatt tttattgttt
     2881 tacgatgatt ttaatctgct attgaaagaa agtcctgatt gctgattgag ttgttaaact
     2941 aatcttagtc agcccactat ccatcattta tttccttagc tgattagttt tcttcttttt
     3001 tgtttttcaa acctagacca ccgagtaaac ctgcaagcgc aagcccaagg aaaccaatac
     3061 ttgccattga tgtttgagtc tcaccagtat ttggtagcat agctttatcc tctgacatca
     3121 tcgtatcaga catcttgtta gcagaagcag ccatgttttc acctgccatc gtgttggtag
     3181 aacttgtcat ggtgtcagca ggcatgctat ctgtagtacc tgtagcatga ttgtgattca
     3241 tcggagtcac gccagaacca gagttagaag gtgataatga accatttgct gtgtctgatg
     3301 tttctttaac atttatctta atagtgactt ttttagttgc tacgatgttg tccaaatctg
     3361 gtttaccgtc tttgttacca tagacattga ctgtagcgct gtaagtttga gtaccgtttg
     3421 ctcggaactg gtcaatgagc gcttgttttt ctttgccagc tacatttccg tccaaggcta
     3481 attgatagaa gtattgacct ttggtcttca cgttttcacc tagtggagat agggctgggt
     3541 ttttagcgtc accgttatct gacca
//
LOCUS       DAVLWA010000124         3520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLWA010000124 DAVLWA010000000
VERSION     DAVLWA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.124"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            335..3454
                     /gene="phtE"
                     /locus_tag="VYN80_001945"
     CDS             335..3454
                     /gene="phtE"
                     /locus_tag="VYN80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1541799.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLVGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     SEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGKKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1314 a          572 c          681 g          953 t
ORIGIN      
        1 tggagacatt gactggtcta aaaagtagtc ttcttctcgg aacgaaagat aataacacta
       61 tttcagcaga agtagatagt ctcttggctt tgttaaaaaa aagtcaaccg gctccgatac
      121 agtagtaaaa tgaatggagc atattttatg gagaagtaac ctttcgtgtt acttctcttt
      181 tttagaaaaa cgtaacagaa acttgacaaa agtaatttta aatagtaaac tatttactgg
      241 ttaattaaat ggttaaataa ccggtttaga aaactattta ataaagtaaa agaagttgag
      301 aaaaaacttc atcatttatt gaaatgaggg atttatgaaa tttagtaaaa aatatatagc
      361 agctggatca gctgttatcg tatccttgag tctatgtgcc tatgcactaa accagcatcg
      421 ttcgcaggaa aataaggaca ataatcgtgt ctcttatgtg gatggcagcc agtcaagtca
      481 gaaaagtgaa aacttgacac cagaccaggt tagccagaaa gaaggaattc aggctgagca
      541 aattgtaatc aaaattacag atcagggcta tgtaacgtca cacggtgacc actatcatta
      601 ctataatggg aaagttcctt atgatgccct ctttagtgaa gaactcttga tgaaggatcc
      661 aaactatcaa cttaaagacg ctgatattgt caatgaagtc aagggtggtt atatcatcaa
      721 ggtcgatgga aaatattatg tctacctgaa agatgcagct catgctgata atgttcgaac
      781 taaagatgaa atcaatcgtc aaaaacaaga acatgtcaaa gataatgaga aggttaactc
      841 taatgttgct gtagcaaggt ctcagggacg atatacgaca aatgatggtt atgtctttaa
      901 tccagctgat attatcgaag atacgggtaa tgcttatatc gttcctcatg gaggtcacta
      961 tcactacatt cccaaaagcg atttatctgc tagtgaatta gcagcagcta aagcacatct
     1021 ggttggaaaa aatatgcaac cgagtcagtt aagctattct tcaacagcta gtgacaataa
     1081 cacgcaatct gtagcaaaag gatcaactag caagccagca aataaatctg aaaatctcca
     1141 gagtcttttg aaggaactct atgattcacc tagcgcccaa cgttacagtg aatcagatgg
     1201 cctggtcttt gaccctgcta agattatcag tcgtacacca aatggagttg cgattccgca
     1261 tggcgaccat taccacttta ttccttacag caagctttct gccttagaag aaaagattgc
     1321 cagaatggtg cctatcagtg gaactggttc tacagtttct acaaatgcaa aacctaatga
     1381 agtagtgtct agtctaggca gtctttcaag caatccttct tctttaacga caagtaagga
     1441 gctctcttca gcatctgatg gttatatttt taatccaaaa gatatcgttg aagaaacggc
     1501 tacagcttat attgtaagac atggtgatca tttccattac attccaaaat caaatcaaat
     1561 tgggcaaccg actcttccaa acaatagtct agcaacacct tctccatctc ttccaatcaa
     1621 tccaggaact tcacatgaga aacatgaaga agatggatac ggatttgatg ctaatcgtat
     1681 tatctctgaa gatgaatcag gttttgtcat gagtcacgga gaccacaatc attatttctt
     1741 caagaaggac ttgacagaag agcaaattaa ggctgcgcaa aaacatttag aggaagttaa
     1801 aactagtcat aatggattag attctttgtc atctcatgaa caggattatc caagtaatgc
     1861 caaagaaatg aaagatttag ataaaaaaat tgaagaaaaa attgctggca ttatgaaaca
     1921 atatggtgtc aaacgtgaaa gtattgtcgt gaataaagaa aaaaatgcga ttatttatcc
     1981 gcatggagat caccatcatg cagatccgat tgatgaacat aaaccggttg gaattggtca
     2041 ttctcacagt aactatgaac tgtttaaacc cgaagaagga gttgctaaaa aagaagggaa
     2101 aaaagtttat actggagaag aattaacgaa tgttgttaat ttgttaaaaa atagtacgtt
     2161 taataatcaa aactttactc tagccaatgg tcaaaaacgc gtttctttta gttttccgcc
     2221 tgaattggag aaaaaattag gtatcaatat gctagtaaaa ttaataacac cagatggaaa
     2281 agtattggag aaagtatctg gtaaagtatt tggagaagga gtagggaata ttgcaaactt
     2341 tgaattagat caaccttatt taccaggaca aacatttaag tatactatcg cttcaaaaga
     2401 ttatccagaa gtaagttatg atggtacatt tacagttcca acctctttag cttacaaaat
     2461 ggccagtcaa acgattttct atcctttcca tgcaggggat acttatttaa gagtgaaccc
     2521 tcaatttgca gtgcctaaag gaactgatgc tttagtcaga gtgtttgatg aatttcatgg
     2581 aaatgcttat ttagaaaata actataaagt tggtgaaatt aaattaccga ttccgaaatt
     2641 aaaccaagga acaaccagaa cggccggaaa taaaattcct gtaaccttca tggcaaatgc
     2701 ttatttggac aatcaatcga cttatattgt ggaagtacct atcttggaaa aagaaaatca
     2761 aactgataaa ccaagtattc taccacaatt taaaaggaat aaagcacaag aaaactcaaa
     2821 acttgatgaa aaggtagaag aaccaaagac tagtgagaag gtagaaaaag aaaaactttc
     2881 tgaaactggg aatagtacta gtaattcaac gttagaagaa gttcctacag tggatcctgt
     2941 acaagaaaaa gtagcaaaat ttgctgaaag ttatgggatg aagctagaaa atgtcttgtt
     3001 taatatggac ggaacaattg aattatattt accatcggga gaagtcatta aaaagaatat
     3061 ggcagatttt acaggagaag cacctcaagg aaatggtgaa aataaaccat ctgaaaatgg
     3121 aaaagtatct actggaacag ttgagaacca accaacagaa aataaaccag cagattcttt
     3181 accagaggca ccaaacaaaa aacctgtaaa accagaaaac tcaacggata atggaatgtt
     3241 gaatccagaa gggaatgtgg ggagtgaccc tatgttagat ccagcattag aggaagctcc
     3301 agcagtagat cctgtacaag aaaaattaga aaaatttaca gctagttacg gattaggttt
     3361 agatagtgtt atattcaata tggatggaac gattgaatta agattgccaa gtggagaagt
     3421 gataaaaaag aatttatctg atctcatagc gtaaggaata gcagtagaaa aagtctgaat
     3481 caaaaatgaa gttctctcaa aagttagaaa taaaactctg
//
LOCUS       DAVLWA010000125         3396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLWA010000125 DAVLWA010000000
VERSION     DAVLWA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.125"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            508..3066
                     /locus_tag="VYN80_001946"
     CDS             508..3066
                     /locus_tag="VYN80_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1541800.1"
                     /translation="MERSQFEEMFQLQSSRLTTQEKLQLFTSVFAGRYDVYAKNFINE
                     QGKIQYFPSYDYGWKQLPPEKRSFQTLTNSVLKSHFRGEAAIGIFPMHLDDSCYFLVL
                     DLDEGDWKEAGLTIRRIARERQMEAHLEISRSGYGLHIWFFFEEAILSRKARLFGKKL
                     LELAMQESMQLSFDSFDRMFPNQDVLPKGGFDNLISLPFQGEAYHQGRTVFVDEQFQP
                     YEDQWRYLQEIQKISTAKVALLIQEELGKQELDKELKIVLSNMIQLEKSSVTPKTLFF
                     LKNMASFSNPEFYLKQAMRQPTYQIPERMYLFGESDHYLWLPRGLLYPLQDKFKQVSV
                     EDRRKVQRSISVEFKGELTFEQELALSDMTSKENGLLHAETGFGKTVLGAALISERKT
                     KTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGREKVIGYVGQYGGTKKWLSKL
                     VDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMFEKVVAQFRGKYLYGLTATPE
                     RKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSFGHLEIEKTKASNFIQLSDWI
                     ATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFEKLLKEKEVDGCYIISGKTKV
                     RERTSLLETLEQLDKGFVLLSTGKYIGEGFDLPQLDTLILAAPFSWKNNLIQYAGRIH
                     RNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDYRVIEGEEKQFVYVDSRYEKV
                     LREDLAGERQECLLILPYVHQTKLMNFLKEFRISQIEICIPETVANKAWLDQLKSQKI
                     KVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTILRLESASIVSELVAGLR"
BASE COUNT         1053 a          508 c          735 g         1100 t
ORIGIN      
        1 agatcttatg ggactttttt tctacccaaa ataggctcca taatatccat agggaattta
       61 cccactacaa atattataga gcccaaagtt ttaggtcgct tgataatatg cgttttttga
      121 attttataga ctgctcgttt aaactctatt tacttcgtac cttctggagc gagacggaat
      181 attagtcaca tacaaaatga gtactattag gattttattt tcatgtacaa tttcagccag
      241 tcttgttata atcagcctat aggaatcaag gaggtgactc ttatggctgt ttttgtgtct
      301 ttggatggaa ttgtggtaga agtccttgat gtcttttctt cttttaatgg ggatagtgag
      361 tttttcttgt gtatagcatt ttgaatctgg aataggacgc catgactgct aaaagatttc
      421 tataaattaa tttgattttc ctaatcaatt tgttcatatc ttatttcatt ccactataaa
      481 cgtcttaaag acaagagtca gtttgttatg gaacgctctc agttcgagga gatgttccaa
      541 cttcaaagta gtcgcttgac gacgcaagaa aaattacaat tgtttacctc tgtgtttgct
      601 ggccgttatg atgtttatgc taagaatttt atcaatgaac aagggaaaat tcagtatttt
      661 ccttcctatg attatggttg gaagcagttg ccacctgaaa aacggagttt ccagacattg
      721 acgaactccg ttttgaaatc tcattttcgt ggggaggcag ctatcggtat ctttcctatg
      781 cacttagatg atagctgtta ttttttggta ctggatttgg atgaaggaga ttggaaagaa
      841 gctggtttaa ccattcgaag aatagccagg gaacgccaga tggaagccca tttagagatt
      901 tctcgttcgg gttacggact ccatatttgg ttcttctttg aggaagcgat tctgagtcga
      961 aaggctcgcc tgtttggaaa gaaactatta gaattggcta tgcaggaaag tatgcaactg
     1021 tcctttgatt cttttgatcg catgtttcca aatcaggatg ttcttcctaa aggtggattt
     1081 gataatttaa tttcccttcc ttttcaagga gaagcttatc atcaagggcg aacggtcttt
     1141 gtggatgaac agtttcagcc ttatgaagat caatggaggt atctacaaga aattcagaag
     1201 atttcaactg ctaaagtggc actgttaatc caagaggagt taggcaagca agaattggac
     1261 aaggagttga agatcgtctt atccaatatg atccaacttg aaaaatcgtc tgtgacaccc
     1321 aagacactgt ttttcttgaa aaatatggct tcattttcta atcccgaatt ttatttaaag
     1381 caggctatgc gacagccaac ctatcaaatt cctgagagaa tgtatttatt tggagaatcc
     1441 gatcattatt tatggttgcc aagaggtttg ctgtatccat tgcaagataa atttaagcag
     1501 gtatctgtgg aagataggag aaaggtacaa aggtctatta gcgtggaatt taagggagaa
     1561 ctcacttttg agcaagagtt agccctgtca gatatgactt ctaaagaaaa tggtttactt
     1621 catgcggaga ctggttttgg gaagaccgtt ttaggtgctg ctcttatctc tgaacggaaa
     1681 acaaaaacaa ttattctagt ccataataag caactcttag accaatggct agatcgctta
     1741 aactgctttt tgactttcga agaggaggag gctatccgtt atacggcatc aggtcgtgaa
     1801 aaggtaatcg gctatgttgg acagtacggt gggactaaga aatggctgag taaactggtt
     1861 gatgtcgtta tgattcaatc tctatttaag ttggaaaata gtcaaagtct tttggatgag
     1921 tatgagatga tgattgtgga tgagtgtcat catgtctctg ccttgatgtt tgaaaaagtt
     1981 gttgctcagt ttagagggaa gtatctttac ggtttgacgg ctacgcctga gcgtaagaat
     2041 ggtcatgagc ctattgtttt tcagagaatt ggtgagatac tccatactgc tgataagagg
     2101 gaaacggatt ttaaacggca attgcaatta agattcactt cttttggtca tttggagatt
     2161 gaaaagacca aagcaagtaa ttttatacag ctcagtgatt ggattgctac agactcagcg
     2221 aggaatcagt tgattctcaa ggatattcta gcccaagtgg cagaaggacg gaatatcttg
     2281 gttttagtta atcgaattca acagatagat gtctttgaaa agttattgaa agagaaagag
     2341 gttgatggct gttacattat tagcggaaaa accaaagtcc gagaaagaac gagtttactg
     2401 gagacgttag aacagttaga taaagggttt gttttgttgt ctactggaaa atacattggc
     2461 gaaggttttg acttacctca gttggacacg cttatcttgg cagcaccctt ttcttggaaa
     2521 aataatttaa ttcagtatgc aggtcggatt catagaaact acaaggataa gtctttggtg
     2581 cgtattttcg attatgtgga tattcatgtt ccttatttag aaaagatgtt tcagaaacga
     2641 caagtagctt atcgaaagat ggattatcgt gtcatcgagg gtgaggagaa acaattcgtt
     2701 tatgttgata gtagatatga gaaggtgttg agagaggact tagcagggga aagacaggaa
     2761 tgtctgctta ttttacctta tgtgcaccag acaaaactga tgaattttct aaaagaattt
     2821 aggattagtc aaattgagat atgtatacca gagacggttg caaataaagc atggctagac
     2881 cagttgaaga gccagaaaat taaagtgtct tttactcaat caaaaatagt aacgcctatt
     2941 cttttggtga ataagactat tgtttggtat ggtgcaatgc cattattagg gaaggtagat
     3001 gagatgacca tattacgttt ggaatcagct agtatagttt ctgaactagt ggcaggttta
     3061 cgatagagaa aatttttaaa aatttctatg tatgattttc atttctttag tgagactgtt
     3121 gccattatca cattcgaatc acacaaaata aaaaaatttt tataagtact tgacaaatag
     3181 attgaaatat cataaaataa aaacggttac agagttatta attatttaag cttcatgtca
     3241 ccattaaaaa ttgaaataaa aggatgttat cactaataca agtgagcagg aacctattta
     3301 atcacatcag aagaagtttc ttgatgtttt taagtaggtt ccttttattt taaaagggaa
     3361 attttatgat cataaaacga atactaaacc acaatg
//
LOCUS       DAVLWA010000126         3394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLWA010000126 DAVLWA010000000
VERSION     DAVLWA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.126"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     misc_feature    77..212
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            251..751
                     /locus_tag="VYN80_001947"
     CDS             251..751
                     /locus_tag="VYN80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1541801.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            827..1195
                     /gene="rplL"
                     /locus_tag="VYN80_001948"
     CDS             827..1195
                     /gene="rplL"
                     /locus_tag="VYN80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1541802.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            1382..1552
                     /locus_tag="VYN80_001949"
     CDS             1382..1552
                     /locus_tag="VYN80_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541803.1"
                     /translation="MQKEGQSSRQFMKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            1552..1800
                     /locus_tag="VYN80_001950"
                     /pseudo
     CDS             1552..1800
                     /locus_tag="VYN80_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            1814..2137
                     /locus_tag="VYN80_001951"
     CDS             1814..2137
                     /locus_tag="VYN80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541804.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSYD"
     gene            2130..2510
                     /locus_tag="VYN80_001952"
     CDS             2130..2510
                     /locus_tag="VYN80_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541805.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            2523..2756
                     /locus_tag="VYN80_001953"
     CDS             2523..2756
                     /locus_tag="VYN80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1541806.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            2759..3346
                     /locus_tag="VYN80_001954"
     CDS             2759..3346
                     /locus_tag="VYN80_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1541807.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKLRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT         1086 a          535 c          702 g         1071 t
ORIGIN      
        1 aaagaaaaat cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt ctttttttga
       61 ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg agctcgactc
      121 ataaatatcc taccgaggac aagacgtatc atgtaaaaag aagcgtattg tactttcgtg
      181 tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa ttacggaggt
      241 gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg acgtagtagc
      301 tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt tgacagttga
      361 gcaagataca gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata aagttattaa
      421 aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg catctgtatt
      481 tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag cgaaaatctt
      541 gaacgacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa tcgaaggcgc
      601 tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg aaggacttct
      661 ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg cagtcaaagc
      721 ggttgcagaa agcaaagaag acgcggctta atcttaagct acacagcgta gtctagctac
      781 gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg cattgaacat
      841 tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg accttgtaaa
      901 agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg ctgcagctga
      961 tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat ctgcaggcga
     1021 caaaaaagtt ggcgttatca aagttgtacg tgaaatcact ggtcttggtc ttaaagaagc
     1081 taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa ctgcagaagc
     1141 tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta aataatagag
     1201 tatcgcaatt tgattgcgga atccctattt accagtcttt gagagcgata agcgattgat
     1261 agaaactgat aaattgattt ggtttcctgc caaaaattct ttggcaggat ttttgtttta
     1321 ataaactttc gggggtagat actagattgt ctaccccttt ttttgtgttt cggaggtaat
     1381 tatgcaaaag gaaggacagt catcacgtca attcatgaag agtcatctca tgactatcat
     1441 gggagttgat attgtaaaag caactcagtt agttgatgaa atggaacaag taggtttaat
     1501 ccgatttgat gaatttggaa atgttggaat tttagtcttg gagggacaat aatgaagcgt
     1561 attaccgcta atcaatatca gacatcagag cgttattata agctccccaa acttttattt
     1621 gagagtgaac ggtataagaa tatgaaaccg gaggttaagg tggtttactc agttttaaaa
     1681 gatcggttgg agttgtcttt gagcaaaggt tggattgatg aggatgggac tatttatttg
     1741 atttattcca attcaaattt gatggcactt ttaggctgtt caaagtcaaa attactctcc
     1801 atgtgagttt gaagtgacat ttttagatga ttaccataaa aaacataact acccactatt
     1861 ttacgaatcc tatcttcaaa acgttatgga attccttgaa agtcaagaca taaagaatgg
     1921 ggttgatgcc tttgtagatg atcatcaaaa tctcgttttt gttttatatg gacaaggcta
     1981 tcgagccgag ggaaaagagg gaatacttac aacccaagta actgtaaaag cttatgatga
     2041 agacaagaaa ccgattaact tcgcaaattt attagattcc ttaatcgtgt cagaatatca
     2101 aatggaaccg aatctttggg aggtctccta tgattgatct ctatctaagt aaaaatagcc
     2161 gaagaaatca acttctttta gacttcttcc aaaactatgg catcgaggta tcttgtcatt
     2221 cagtttctga aatgacaaag gacaaattaa ttgagatgat gagctattct tcagattgtt
     2281 ttgagttttt atctccaaat ttattacgtt ttaagaatcg agataaccta aaattaacag
     2341 atttcatcga aatgatatta aaaaatcctg aactaaccat cagacttcct cttgcggttt
     2401 caaataagcg agtttatcca agtctgaatt tggaagaggc tagagctcta ttgccaagag
     2461 atacgaaaca attgatttac atggcgcaaa cacattattt atctaactaa aggagaagca
     2521 atatggctga acgattttgg gagaacttgt ccattatttt ggctgaaaga aatatcagct
     2581 ggattgagct aaccagaaaa atgtttgcgg gagagtttca ctatccaagt gaattgaatc
     2641 gtttgtatca aaagattcgt cactacaaga tggaacaacg aatgcctcaa agtccatggg
     2701 tagaaaggat tgtgcaggta ttagatttag attatgaaga tttatttcgg aggtaactat
     2761 gaaaagaata attccagttt atatattcca acaagtaaat gtcctattgg tatctctata
     2821 cttactgaaa tttctttgta tcggtgagtt aactatacta cagattctat attgtgcgtc
     2881 actcatttct tttttatgga tgtatgccca acgtaaacaa gcgcataagg tcaatatgaa
     2941 attaaggata aaatggattg gtattggatt agccagtcta ctgattataa gtctatgttt
     3001 tagtctaatc catgcgcaag aaagcacgaa tcaagcaaac ttaattgggc ttcaatatca
     3061 agttccttgg ttttcatttt tattgttctt aatcaatgcg agtatggttg aagaatttct
     3121 gtatagagaa attttatgga acttggttag aaaattagat attcgagttg ctttgacaag
     3181 tgttttattt gccttagcac atcatccagg aaccattata gcttggtgtt tgtatgtatc
     3241 gctggggatg tttttagggc ttgtacgcta caaattggac ttatggggca gtatggggct
     3301 acatttggtg tggaactttt tagtttatag tttgctgctt ttttaaaata ggtgacctga
     3361 tttatctcat aaatctgtag tttttatggg attc
//
LOCUS       DAVLWA010000127         3244 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLWA010000127 DAVLWA010000000
VERSION     DAVLWA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.127"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(165..419)
                     /locus_tag="VYN80_001955"
     CDS             complement(165..419)
                     /locus_tag="VYN80_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1541808.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(439..2280)
                     /gene="typA"
                     /locus_tag="VYN80_001956"
     CDS             complement(439..2280)
                     /gene="typA"
                     /locus_tag="VYN80_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1541809.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2442..3167
                     /locus_tag="VYN80_001957"
     CDS             2442..3167
                     /locus_tag="VYN80_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1541810.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          959 a          715 c          619 g          951 t
ORIGIN      
        1 ataatatgca accagtacta accaaatata aaatagatgc cattaacgaa ttttattcaa
       61 gtttttccca ttcatactat acaagtaaaa gagatggtgt taactaaaaa gcaattcaaa
      121 ctattgtaaa attcctagca aaaagagagc cgaaactctc ttttttatct tcttttactt
      181 tttttgactg gcatgagtgt gatgtctctc acactaaagt aagctaggat caacatggct
      241 attgctagga atatttctgt tggtaattga aaaattttca gaaaagatag aaccaataaa
      301 atcaagagtg ccactaaaat acataccata gcgacgatat tgacagtccc tttaatgctt
      361 tctggtgtcg caaatacata gagtaggagc agtaaaattc ctaggactaa atagaccatc
      421 tttctctctt tctagctctt attcagctga ttttttcttc ttgttagctt tctcacgctc
      481 tgctttgtta aggatttgtt tacgcaaacg gatagactca ggcgttactt ccatgtactc
      541 atcgtcgttc aagaactcaa gagactcttc aagtgtcaag atacgaggcg tcttgataac
      601 agctgtttgg tccttagtag ctgaacgaac gttggtcatt tgttttgcct tcgtgatgtt
      661 aactgtcaag tcattttcac gagagttttc accgatgatc attccttcat aaacctcagt
      721 acctgggttg acaaagatcg taccacgttc ttcgatagac atgattgagt aagttgtagc
      781 cttaccagca tcgatagaaa caagggcacc acggtgacgt ccaccaattt cccctggaat
      841 caatggcaag tattggtcga aggtatggtt catgataccg taaccacgag tcattgacaa
      901 gaactcagtt gagtatccaa tcaaaccacg cgctggaaca aggaagacca aacgagtttg
      961 accattacca gttgaaatca tatccaacat ttcaccttta cgttcagaaa ggctttggat
     1021 aacagaccct tggtattctt ctggagtgtc gatttgtaca cgttcaaatg gttcacattt
     1081 aacaccgtcg atttctttta cgataacttc tggacgagat acttgaagtt catagccctc
     1141 acgacgcatt gtttcgataa ggattgacaa gtgcaattct ccacgtcctg aaacagtcca
     1201 tttatctggt gaatcagttg ggtcaacacg aagggaaacg tctgtttgca attctgcctg
     1261 caagcgttct tccaccttac gagaagttac ccatttacct tctttaccag caaatggtga
     1321 gttgttgacc aagaaagtca tttgaagagt tggctcatcg atgtgtagga ttggaagagc
     1381 ttctactgca tctgtcggag tgatggtttc accgacaaag atgtcttcca tacctgaaac
     1441 ggcaatcaag tcacccgctt tggcttcttg gatttcacga cgttccaaac caaagaaacc
     1501 gaagagtttt gtaacacgga agtttttagt tgtaccgtca agtttagaaa gggtaacttg
     1561 gtccccaacc ttaactgtac cacggaagac acgaccgata ccgatacgtc caacgaagtc
     1621 attgtagtcc aaaagtgaca cttggaactg caaaggctca tctgagttat ctactggagc
     1681 tgggatatgg tcgataatcg tgtcaaagat tggtgccata gtcgcttctt ggtcagctgg
     1741 atcatctgac aatgaagaag ttccgttgat cgctgaagca taaaccactg ggaaatcaag
     1801 ctggtcgtca tctgcaccaa gctcgatgaa aagttccaag acttcatcca ctacttctgc
     1861 tggacgagct gatggcttat cgattttgtt aacaaccacg attgggacaa ggtcttgttc
     1921 caaggctttt ttcaatacga aacgagtttg tggcatggtt ccttcatagg catctacgac
     1981 caagacaaca ccgtcaacca ttttcatgat acgctcaact tctccaccga agtccgcgtg
     2041 tcctggtgtg tccataatgt tgatacgagt tccgttgtaa gcaacggcag tatttttagc
     2101 aaggatggtg attccacgct ctttttcgat atcgtttgag tccatagcac gctctgccaa
     2161 ttcagtacgt gcatcaagcg tttctgattg tttcaataat tcgtcaacca gggttgtttt
     2221 accgtggtca acgtgggcga taatcgcaat gttacggata tcttctctta attttgtcat
     2281 gatttcctct ataatattca aaatttattt tctaactgaa cgattatacc ataatttcaa
     2341 ataaataaca taactcaagc aagtgtaaat gttttcactc cgcttttctt ttcacgtcaa
     2401 gccttttcaa agcaagcgac ttatgataag ataggcacag tatgcgttta gataatttat
     2461 tagcccaaga aaaagttagc cgaaaggcca tgaagcaagc actcctcaga gggaaaattc
     2521 tagtcgatgg ttgcccagcc cgctccctag ctcaaaatat cgatacagga ctacaagaac
     2581 tcctttttca ggatcgaatc attcaaggct atgaacacac ctatcttatg cttcataaac
     2641 ctgctggtgt cgttacagcc aacaaagaca aggaacttcc gaccgtcatg gacctgcttc
     2701 catctgacat ccagtctgac aagctctatg ccgttggccg actggaccga gatacaacgg
     2761 gactcctcct cttgaccgat aacggtccct tgggcttcca actcctccat ccccaatatc
     2821 atgtcgataa gacttatcaa gttgaggtta atggacttct aacacctgac catatccaag
     2881 cctttcaaaa gggaattgtc tttttagatg gtactgtctg taaacccgca aaactagaga
     2941 ttctatctgc aagtccctcc atcagtcaag cctctatcac catttcagaa ggaaaatttc
     3001 atcaaatcaa gaaaatgttc ctctcggttg gtgttaaggt gactatcctc aagagaatcc
     3061 aatttgggga cttcacattg aacccagatt tagcagaaag taactaccgc cctttgaacc
     3121 aaaaagagtt acaaatcatt aaaaactatt tagagatgag tcgataaaac aaaaaaagct
     3181 ttaaaactaa agcttttttc ttttatttac cgaaaaatta aggcgattgc tacaatccag
     3241 ttaa
//
LOCUS       DAVLWA010000128         3178 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLWA010000128 DAVLWA010000000
VERSION     DAVLWA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.128"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            99..830
                     /locus_tag="VYN80_001958"
     CDS             99..830
                     /locus_tag="VYN80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541811.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            845..1996
                     /locus_tag="VYN80_001959"
     CDS             845..1996
                     /locus_tag="VYN80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1541812.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRASNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSICLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(2048..2707)
                     /locus_tag="VYN80_001960"
     CDS             complement(2048..2707)
                     /locus_tag="VYN80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1541813.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            2777..>3178
                     /locus_tag="VYN80_001961"
     CDS             2777..>3178
                     /locus_tag="VYN80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541814.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT         1166 a          467 c          652 g          893 t
ORIGIN      
        1 tactctgaca cagaacttta agaaaaagat aattattgta gtaatttctt taagaaaatg
       61 atataattca ttaaaaagta tttggaggag gaagaattat gttgaatcag gatctctttg
      121 attcgcttga ggcacaaaaa attgtagata ctttgatgaa agggcaaaaa gattatgtag
      181 atgaacgtct agaaaaaaga gagacaatga tagtgtcgaa tggttatgca tggacgcgac
      241 ctaatcatat tgatactgca tttgcatcag cagatttgtt tgagtataaa ttacaattag
      301 caggacagac ttggggatat ttagaatttg aaacaaatac agaaaaatat gggaaagtat
      361 tgttaattat aaagggtaag aagcgactta cgaaccaatt tcctttggta caaaaaaata
      421 agagtggcta cttatttgaa tacgctcaga tgaatacact ttatcttaat caacattctt
      481 cctacaaaaa tgatgaagat agtcattcct ttccaattca gatggagtta gtttctgatg
      541 aaatgattca agaaattgaa caagctacta aaaattcgaa tatcgaaaaa tttatgattt
      601 taacttatga ggcggactca gaaaacaata ttatatctgt agatgttgtt atgcctgatg
      661 cacgaactgg tcagctacac ttgattcaag atttgtctga gtatattcaa tcaagttcgt
      721 accatttcga agaagctaaa tatcaagata ttcctaattt ttcagaatta tcggaaacag
      781 aagattttga aattattcca agaatagaaa aacaagaagg tcaaaagtag taaggagtat
      841 ataaatgttt aatggtcggg tattgaaaga attacggctg ttaaatggtt taagtagagc
      901 agaattagct cagagaatta atttaacgga acaagccatt tggcagtttg agtccaacga
      961 aacgaaacct aaattatcaa ccaaaatgca tttggccaac caatttcatg ttgatttaac
     1021 ttattttgaa caggaagaag agagcattcg atttgattct tctgtaattg cctttagaaa
     1081 tgcagaccta gcaacacgga aaacaataga tattcaaact atgtatttac ataaggtaga
     1141 tagtttgatt gattattttg aaagttttgt aattatacct aatattataa ttcatgacct
     1201 aagtaatgta gtgagtgaat cttatcataa gggagaatcc attgaggaat tggctcttta
     1261 tgccagggaa aaattaggta tttcaaaaga taatcatgat ttgctttata aattagaacg
     1321 ttcaggtatc tatatcgtgg aacgattaat taatggccaa gctgatgctt atagcgcatg
     1381 gtcaaaattg ggaagacctt atattgtgtt gggaacgaat aaatcatctg tacgtcgaaa
     1441 ttttgactta gctcatgagc taggacatat tcttttacat aaatataagg atatgaatga
     1501 agatggcgat cgtttggagc aagaagcaaa ttattttgca tcatgttttt tattgccaaa
     1561 agaagagttt ttagtcaaat ttgaagagag ggttggcaag cgtgccagca atcctgatag
     1621 ttatatttta ttgaagtcgg atttgaatgt ttcgatacag gctttagagt atcgagcttt
     1681 taagttagga ttattgactc caaagcaaca ttcttacttt tatcgtcaaa ttgcgcaaaa
     1741 aggttacaaa atgattgaac ctttggatga tcaaattttt gttaaaaaac caagcaaagt
     1801 aaagagtatt ctggacgtcg ttttgagtaa tcatctagtc agtctagcga ctataatgtc
     1861 taaacaaagt atttgtttac agtttataag cgaaatattt tcagtcgaaa tgaaattttt
     1921 tgatcagtat caagaagata gaagaacaga tcgatttgat aacatcatcc ctttgtacaa
     1981 aagaaataat ttataattta actgtgaaat aatttcatga aggacatatg gcagaaattg
     2041 gattttatca gacaagcagt agcttaccaa aaataatcaa acaagtagca agtaagagga
     2101 aaggcacgaa aggaagtctt tcctttttct tttgcaggat aaaggccagg atacccgtcg
     2161 cagaagcaaa ctgaatcaag atcagtaact ccgttacgct aaagacgaga gcacaagaag
     2221 ctaaaaagag gaaatctcct gcgcccatgc ggatatcgat aaaatgagcc aaaattccaa
     2281 gagcaaggaa ggagaccatg accagattcc agccagagga agctattagg attaggtgga
     2341 aagtcatcca gaccagtaag ggatattcct gatggtgaaa gtcgtagata cccaaggtca
     2401 aaccagcggt gattaggacg acttgcccca aggaaagcaa ttcccaagag taaagcagaa
     2461 agaggagtcc taagactaat tcaaagaggg cataccagac aggatagcga actttgcagt
     2521 agcgacagcg aaagcgattg aagacctgtg agagaatcgg aatcaaatct aagggacgca
     2581 agcgagtctg acaggaatcg cagtgactgg ctgaactgat aatggattgc tctggaaaac
     2641 ggtcaatgac caaaccaaga aaggaagcga gaatgctccc gacgagaaaa aaataaaaat
     2701 caatcatact tatctattcg taaaaaatgg gagaaatagt ataatggaga aacatagata
     2761 agatggtgag gaaaagatga caattcgttt tgaagaaaag gtgagtatag aaaacgctca
     2821 atttgtatgc cagtggtcca actccctagg caaatccttt caagaacaat ggatgggacc
     2881 aaggattcct tttctactga cccttcaagc cttggaagga gtcttttcaa tctttgatga
     2941 acaagagttt gtgggactta tccagaaaat caggctagaa gacagcaatc ttcatatcgg
     3001 gagatttttt atcaaccccc agaaacagga gcaagactta ggtagccagg ctttaaggaa
     3061 atttgttagt ttggcctttg aaaatgaaga tatagatagt atttctctaa atgtcttcga
     3121 ggcaaatcaa agagctcaga atctttacca aaaagaagga tttgaaatcg ttcaaatg
//
LOCUS       DAVLWA010000129         3109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLWA010000129 DAVLWA010000000
VERSION     DAVLWA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.129"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            86..1156
                     /locus_tag="VYN80_001962"
     CDS             86..1156
                     /locus_tag="VYN80_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV1541815.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            1211..2352
                     /locus_tag="VYN80_001963"
                     /pseudo
     CDS             1211..2352
                     /locus_tag="VYN80_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2435..2528)
                     /gene="ffs"
                     /locus_tag="VYN80_001964"
     ncRNA           complement(2435..2528)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYN80_001964"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2561..3028)
                     /locus_tag="VYN80_001965"
     CDS             complement(2561..3028)
                     /locus_tag="VYN80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1541816.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
BASE COUNT         1147 a          610 c          492 g          860 t
ORIGIN      
        1 tactacaatc taaaaaccat gacaaatgaa atcatggtta aagaagagta tttagcatta
       61 gtaaattaaa aaaaggagta tcaatatgga aattgataaa gtaaaagccg atttaaaaca
      121 agtcggcaaa cgtgtagcag acctcagcca aagcattacg aatgaagaac aaacaaagaa
      181 tgccttcatt atgccgttct tccaagcgct tggatatgat atcttcaatc cacttgaatt
      241 tgtcccggaa tttactgccg acgtgggcat taaaaaaggc gaaaaagtcg actatgcgat
      301 cattttggat ggcgagcctc aaatcctaat tgaatgtaaa tcaattacag aaaaccttac
      361 taagcacgac tctcaactat tcagatattt cgtaactact aaatcaaaat tcggtatctt
      421 gacaaatggt agagaatata aattctttac tgatttagat gagccaaata aaatggacac
      481 aactccattc ttaacaatcg atgtgactga tatcaaagaa aatcaattta ctgaaatcat
      541 caaattccac aaagagaatt ttgatattga taatattgtt tcatcagctt ctgaattgaa
      601 atacctcaac aatcttaaag cgttccttac tgaaaatatc actacaccgt cagatagctt
      661 cctcagatat ctaacttctg aaatctatga agggcgtgtt actcaaaata tcttaacgac
      721 attttctccc attattgtga aagggtttaa tcaattcatc acagagcgag ttaatgaaaa
      781 attaagtgct gcacttaata caagcgttga aaccaaagta acaaccgata ttccaaaagt
      841 tgaagctgaa gctgaagaga ttgtcgaagt aactgacgaa attatcacaa ctcctgctga
      901 attagaagtt tatactgttg ttaaaatgct tgctagggat gtagtttctc cagaacgtgt
      961 attttacaga gataaccgta gctatttcaa tgttttggtt gacgataaca tcaaaaaatg
     1021 ggtattacgt tatcgttcaa attcaaaaaa aagcactatc gaaatccgtg ataaaggcat
     1081 cttcccggtg tctacccctc tcgaagtcgc aaattatgcc aatgaaattt tagaagtaat
     1141 caagaaattc tcataataaa aaaagccctc tttactatac ccattttatc aaaaaagtga
     1201 ggtaaaatca atgtggatgg aagaattgcc aaacggcaaa tataaatttt ttgagagata
     1261 caaagatcca tatactgaga aattaaaaaa agtctcagta acgatggaga aaaaaactcc
     1321 ccaagcaaga aatcaagctg cgattttact tcaggaaaag ataaaacaaa agttaggaga
     1381 aaaacaacat tctgtttcta atataacttt tgaaaaacta tatgaagaat ttgaggagaa
     1441 ttggaaacac ggcgtaaaaa actcaaccgt ctacgcttca aagaatgtaa aaaaagagat
     1501 tttaaagcag atagagggcg actacctagt tagaaatttg atagacgttt attacaaaaa
     1561 gtaatagatc aactattaca agatgggaga tctcataact atgtttctaa aatcaaattc
     1621 aagctcaatc agataatgaa atttgctatc agaatgaatt atattgatac aaatgaaatg
     1681 ctatttgttg aaacgcctag aaaagtaatt acatccgacg aactcagaaa gaaaaataca
     1741 aaatacttag accaaaaaga gtttaagtta ttcatccaaa atttaaaaga cgaggcccta
     1801 tgtgattatc gaattacaaa gtatatccga atagctaaag ttctttttct tactggcatg
     1861 aggtatggag agctggcagc cttaaactac aaggaagata tagatttttc taaaaagact
     1921 attcacatca agcatacata cgatttcaga caaaaagaga gaactacacc aaagacaatc
     1981 aagtccgata gggttataac agcacctcaa aaagtgttag atattatcaa agagcaaata
     2041 atagagaatg cgacaaatgg atttgataca gattttattt tcatcaatac tctaggagaa
     2101 ccaataacaa atgccagggt tatttgtgca ttgaaaagac atggtcaaaa aatcggcata
     2161 gaaaaaaaca taactacaca tacatttaga cattctcaca tatccctact tgctgagctg
     2221 ggcattccct tgactgccat catggacaga gtagggcata gtgactcaaa gaccacacta
     2281 gagatttatt ctcacgttac tcaaaaaatg gtatcagaca tatctagcaa gttagacaag
     2341 ataaaatttt aaattgtgcc cctcgtctgc ccctttttct tatacaagac aaacaaaaac
     2401 cccttaaagt gttgatttta aagggttttt aaagtgcacg aaaaaagagc acacaattca
     2461 aatcgcttag ggctgctgga ttcctcccct gacccgcttc acgcagaact gttgctccac
     2521 tatttattat atcacattcc tattcatttt aaaagcaaaa ttattttttc cgtctatttc
     2581 taaaaaaatc ctgcatgata gctgcgcatt catcttccaa aattcctatt tcaacctcca
     2641 cacgatgatt gagacgctca tctgtcaaga tatcgtacaa actcccagca gcgccaaatt
     2701 tctggttttt agccccatag accacatttg gaatgcgggc aagcccaatc gccccactac
     2761 acatgacaca aggttcaatg gtcacaaaaa gtgtgcaatc cagcaagcgc cagctctcct
     2821 cactcaagtt cgcatcctct atagccataa tttccgcatg cataaccgct cgctgtaatt
     2881 cctcacgcgc attatgccca cgaccaatga tttccccatc tttgacaatc acacaaccaa
     2941 ttggaatttc atcgtgttca agagcaatct cagcctctct caaagcctcc ctcataaaga
     3001 cttctttttc ttcaagcgta taatacatct cttttctctt ttcctactta tcgattttat
     3061 tattatatca tgaatcccaa cacaaaagaa gccaccgaat gcggtgact
//
LOCUS       DAVLWA010000130         3051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLWA010000130 DAVLWA010000000
VERSION     DAVLWA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.130"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(193..921)
                     /locus_tag="VYN80_001966"
                     /pseudo
     CDS             complement(193..921)
                     /locus_tag="VYN80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1181..1492)
                     /locus_tag="VYN80_001967"
     CDS             complement(1181..1492)
                     /locus_tag="VYN80_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541817.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1507..2793)
                     /locus_tag="VYN80_001968"
     CDS             complement(1507..2793)
                     /locus_tag="VYN80_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1541818.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          907 a          672 c          535 g          937 t
ORIGIN      
        1 ttttcaaaat atctaagaca tcgcttcttt gtttaatttg ataggcttta atacctaagc
       61 tccgacttta tcacttaaaa aataaaaaag gacagctaat ctcttgcgca tcaagggatt
      121 tcgccattct ttaatctcgt ttaagtgttg tatatggagc tttgcataat tttttaaaac
      181 taaacaacta cattaagcac gaactgtgac gctggttcca tcttccttat aaagattgat
      241 aagcccctct ttaagagctc gaaccatgtc tccagcttga acaagttgtg ggactttaat
      301 agtcaatagt tccattggat ttggagcgcg gtcgatttta ttgcctttgg catcatgcaa
      361 atcttcaata taggtttcaa aatgacggaa acctggacca taaaactcaa cttggtcccc
      421 ttcgttaatg acgttccgtt gacgaatggt tgctgtttgt gtcgcatcat cataagaaac
      481 cacttcagcg acaaacttgt actcagggat tttacgacga gcaccaaaca actgctcatt
      541 ttcagatggt gtaccatagt aaaatcctgt agccagttca cgttgggcaa ccttccacat
      601 ctcgtccacc aagtcttgtt tgatagcttc aaacttttca ggactttcaa gataggcatc
      661 cacagccgcc ttgtagcagt tggttactgt tgatacatag tgaatagact ccatacgtcc
      721 ttcgattttt agactgtcca caccattttc aatcatatct gagatatggt caatcataga
      781 catatcaacg gctgacattg aaaattcttc tggaatctca ccctgcaaac tcttacgttc
      841 tttcccaaat ggcatatcgt aaaggtcgta cttccaacgg catgactgag aacatccacc
      901 acggttggca tcacgcatac tctagacgga ttgttttgac aattctaacc ttttagactt
      961 tgcataataa ctgttaaaca gtcgaaaatt accccagttt taacaagtag tcaaaatacc
     1021 agataaagta aaaaggggct aggaaaaaag tctttaaaat cttaaaaacg catagtatca
     1081 ggtattgaac aatcttgata ttatgcgttt tattgtggga agatttactt cattttctct
     1141 tggaattgag ttttctccta cttcaaaact agatgatttg ctatttaaaa tagcgttcct
     1201 tatgattttg gcaacctgga ttaaagcgag aaaaacaaaa ggggcaactt aagctttctt
     1261 tatattgctt atatgttagt agttttagac aaacaccaca taaaataggc ttatcctgta
     1321 tcaaagataa gggatacggc tcaaaagggt gattttctaa tgaatcatga caccgataac
     1381 aagcataata ctttttacaa tcatagcatt gtaaagcaat aatatccagc ttgctatggt
     1441 aatgaatgca tcttgtttgc ttatccacta agaaaccctg tgcctctatc atttcctttt
     1501 cctttcttaa gctcgaactg tgacgctggt tccatcttcc ttataaagat tgatgagtcc
     1561 ttcttttaat gcacgaacca tgtctcctga ttgaacgggt tgaggcacct taatagtcaa
     1621 tagttccatt ggatttggag cgcggtcgat tttattgcct ttggcatcat gcaaatcttc
     1681 aatataggtt tcaaaatgac ggaaacctgg accataaaac tcaacttggt ccccttcgtt
     1741 aatgacgttt cgttgacgaa tagttgctgt ttgtgccgca tcatcataag aaaccacttc
     1801 agcgacaaac ttgtactcag gaattttacg gcgagcacca aacaactgct cattttcaga
     1861 tggtataccg tagtagaaac ctgttgccaa ttcacgttgg gcaaccttcc acatctcgtc
     1921 caccaagtct tgtttgatag cttcaaactt ttcagaactt tcaagatagg catccacagc
     1981 cgccttgtag cagttggtta ctgttgatac gtagtgaata gacttcatcc gtccttcgat
     2041 ttttagacta tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatgtc
     2101 aacggctgac attgaaaatt cttctggaat ctcaccctgc aaactcttac gttctttccc
     2161 aaatggcata tcgtaaaggt cgtatttcca acggcatgac tgagaacatc caccacggtt
     2221 ggcatcacgc atactcatgt ggtttgaaag agtacaacgt ccagagtatg aaatacacat
     2281 agctccatgg acaaaggctt caatttcaac atctgtacgt ttgcggattt cagctaattc
     2341 ttccattgaa acctcacgcg ctaaaacgac acgagtcaag cctagctctt tccagaactc
     2401 aagggtttca tagttagtgg cactggcttg ggtagaaagg tggatttcaa ggcctggtgc
     2461 ttcagtcgct gcaatcataa tcaaggctgg gtcagatacg ataactgctg caatcccgat
     2521 atcacgcagt ttacggaacc actcaccagc accagcttca tttccttcgt gcataaccat
     2581 attagccgct acatagacct tggcaccata cttggccgca aactgcacgc cttcttccat
     2641 ctgttcgaaa gtaaagtttc ccgcacggct acgaagacca taggcctgac caccgataaa
     2701 gacagcatct gctccatact gaacagctac ctttagcttc tctaaagtcc ctgcaggtga
     2761 taaaacctca ggacgtttta atgtttttgt catgatttct cctatttgca atataaaagt
     2821 ttgacatttt tccttctcat tttatagtaa ataaaggata aaatcaagcc tagacggatt
     2881 gttttgacaa ttctaatctt ttagactttg cataataact gttaaacagt cgaaaattat
     2941 cccagtttta acaagtagtc aaaaagtcta gaaaagtaaa aaccagtatc caaagatact
     3001 gactcgtaaa aatacataaa ttagtcctta gatgactact cccactctgt t
//
LOCUS       DAVLWA010000131         2996 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLWA010000131 DAVLWA010000000
VERSION     DAVLWA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.131"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(76..537)
                     /locus_tag="VYN80_001969"
     CDS             complement(76..537)
                     /locus_tag="VYN80_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541819.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(504..647)
                     /locus_tag="VYN80_001970"
     CDS             complement(504..647)
                     /locus_tag="VYN80_001970"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1541820.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            916..1296
                     /locus_tag="VYN80_001971"
     CDS             916..1296
                     /locus_tag="VYN80_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1541821.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1395..1616
                     /locus_tag="VYN80_001972"
     CDS             1395..1616
                     /locus_tag="VYN80_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1541822.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1633..1947
                     /gene="trxA"
                     /locus_tag="VYN80_001973"
     CDS             1633..1947
                     /gene="trxA"
                     /locus_tag="VYN80_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1541823.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2322..2786
                     /gene="queF"
                     /locus_tag="VYN80_001974"
     CDS             2322..2786
                     /gene="queF"
                     /locus_tag="VYN80_001974"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1541824.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
     gene            2848..>2996
                     /locus_tag="VYN80_001975"
     CDS             2848..>2996
                     /locus_tag="VYN80_001975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541825.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSLKHCFEVADRTDEVSLK
                     HCFEVV"
BASE COUNT          939 a          625 c          564 g          868 t
ORIGIN      
        1 ttataaagac acgcaaatga gtataaatca gctaattttt ggtttacatt cctattttta
       61 tgcataatta ttctgtcatt ctctaatttg tgaatcaata tatctgatta gaaaactacc
      121 attgagtttt tgctccacct cattagtccg ctcaaaacgc cgtccgctaa tcttgtcata
      181 gactttctcc cctctaggag cccctgcata aggacgatag aggtagcggt tgattcccgt
      241 ttcgtcaata tataggactg gtaaatcttt taggttatcc aaaatatcaa gaaactcaga
      301 aactttctca ggcttttgtt ccctaaaact ggtcgtcttt tttaaaatga ccttaatctg
      361 ctttaaaact gcccatacgg agggcaaagc acaatcaaaa cgggccgcaa tttcccgtaa
      421 aaaagcgtct ggatgagcct ctacaaaggc tttcaattct tctaaaggga tcttacgctt
      481 tttgacgact cgctttttcc gctctaagtt cccttgctca cgtctttctt ttcccacgtg
      541 aagagagttc tgacgccgac atcaaaaact ttggccgcct cgacatgtct gtgccctcct
      601 ttgatggaat ctaatgctcc ttgtttaaaa tcagtactat atgccatagc tttattagac
      661 catgcttatt ttaaactaag caactataga tagagaaatg aaataaaatc tgaattcgaa
      721 tgtgataagg agttagaaat tgaaacaata ggattccccc attattcaca caaaaataaa
      781 accactaaaa aacagagctt gccagcattc ttgaaaagta gtataattat tgcatgcgca
      841 atcatcttgt gacgcagaga ctgcccgtta atggactttc ttctatttta tgactctaaa
      901 aagaaaggag acactatgac ctatttggaa aaatggtttg acttcaatcg gcgtcagaaa
      961 gaaattgaaa gtctcttgga agagaccatt gcccaacaga gcgagcaaag tctgaccttg
     1021 aaagagttct acctgctcta ctatctggac ttggctgaag aaaaatctct acgccagatt
     1081 gacctgccag ataagcttca tctgagcccg agcgcagttt ctaggatggt ggctcgcttg
     1141 gaagctaaaa attgcggtct actcagtcgc atgtgttgcc atcaagatag acgctctagt
     1201 tttatctgcc tgacaaatga tgggcaaaag acactggcct cgctacaaaa gactgtcgaa
     1261 gaaagcttgg aagcaggttt ggatttcttg atttaagatg atttttaaaa aagttgcgtg
     1321 cgcaatcatt tttcttgaca ttctctttta taaggagtaa aataaagtta gcattaaaca
     1381 aaggagtttt aaatatgatt gaaattacct atatagatgc cagcaagaac gaaagaactg
     1441 tgactttcga gtcttatgaa gactttgaac gttcgcaaca agcttgcctt atcggcgtcg
     1501 cagactacta ccctgtccaa aaattaacct acaagggtca taatttggac taccatggga
     1561 cttatggaga tatcttcttc tatctcatga aacaagattt aagccaatat aactaaaaaa
     1621 aggagaaata caatggcaaa agcaattaca gatgcaacat tcgaacaaga aacaaaagac
     1681 ggtttggtct tagtagactt ctgggcaact tggtgtggtc catgtcgtat gcaaggtcca
     1741 atcttggaca aattgtctga agaactttca gaagatgtct tgaaaatcgt taaaatggac
     1801 gttgatgaaa atccaaacac agctcgtgct tttggaatca tgtctattcc aactcttctc
     1861 ttcaaaaaag acggccaagt tgtcaaacaa gttgcaggtg ttcacacagc agaacaaatc
     1921 aaggccatca ttgctgaatt gagctaatca aacgagagac caagtacttt ctttggtctc
     1981 ttttttcttg ccctttgcca tttttcaaaa aatatgctag actatagtag attgaaacta
     2041 gaatagtaca tctctacttc taaaacattg ttagaaatcg atttgactgt cctgatcgat
     2101 ttgtcctgtt cttatttcac tttactatat aaatagaata ttacgatatt tgggatatgc
     2161 catcgctaaa aaaaacctcg atttggtatt ttttagctcc ctaatgggag ctttttacgt
     2221 gctctgaaca tttcccattt tggaaggagt actatgaaac gtcaatcagc cttggtcgtc
     2281 ttagtggtgg tcaagattca aaaacctgcc ttttctggac catgcaacac tatgaaacag
     2341 tcgaagctgt cacctttgcc tacggccaac gtcatcacct cgaaattcaa attactagag
     2401 aaatcgctaa ggaacagggc attcgtcacc atatcctcga tatgtctctg ctgggacaaa
     2461 tcactgctca gccagacttt gcgacgattc atatttccta cattcctgac aagctctgtg
     2521 tcgagtcaaa atccctcaaa ctatatctat ttagctaccg aaaccacgga gatttccacg
     2581 aaaactgtat caacaccatc gggaaagact tggtcaactt gctagaccct cgctatttag
     2641 aagtctgggg aaaattcact ccgcgcggtg gcatttcaat cgacccctac tataactacg
     2701 gtaagcaagg aactaagtat gagggcttgg cagaacaacg cctcttccaa cacgaccttt
     2761 atccagagaa aattgacaac cgctaaagtc atactcaatg aaaatcaaag agcaaactag
     2821 gaagttagcc gcaggttact caaagcagtg tttgaggttg tagataaaac tgacgaagtc
     2881 agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagctt aaaacactgt
     2941 tttgaggttg cagatagaac tgacgaagtc agcttaaaac actgttttga ggttgt
//
LOCUS       DAVLWA010000132         2951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLWA010000132 DAVLWA010000000
VERSION     DAVLWA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.132"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            17..157
                     /locus_tag="VYN80_001976"
     CDS             17..157
                     /locus_tag="VYN80_001976"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541826.1"
                     /translation="MADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFDMAKTQIE
                     TD"
     gene            249..1928
                     /locus_tag="VYN80_001977"
     CDS             249..1928
                     /locus_tag="VYN80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1541827.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEERQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            2196..2762
                     /locus_tag="VYN80_001978"
     CDS             2196..2762
                     /locus_tag="VYN80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541828.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1114 a          371 c          636 g          830 t
ORIGIN      
        1 caatgactgg aatggattgg ctgatttaat agcaaatctg attgcaaaat atgctggtgc
       61 tttagattta gataatctac ccgatccaac gccagcaaaa aatcaagaga tgaaaaatag
      121 ttttgacatg gcaaaaacac aaattgagac ggactaataa aagtgttata atatacttga
      181 aatgatagtc caaacttaaa ttaaaaaaat tatatatgat atagaaaaat tatattgagg
      241 ttaatactat gtcaaaagaa aaaataaaag tatacctcta tacacgagta tctacgtcaa
      301 tacagataga ggggtattcg ttagaggcac aaaaatcacg aatgaaagct tttgctattt
      361 acaacgatta tgaaattgtt ggagagtatg aagatgccgg gaagtctggc aagtctattg
      421 aaggtagaat acagttcaat cgaatgatgg aagatataaa atctggaaaa gatggagtat
      481 cttttgttct tgtgtttaag ttatcaagat ttgcaagaaa tgccgctgat gttctttcta
      541 cacttcagat aatgcaggat tatggagtta atcttatctg cgttgaagat ggcattgatt
      601 catctaaaga tgctgggaaa ctaatgattt ctgttttatc agctgtggct gaaatagaaa
      661 gagaaaacat ccgtattcaa acaatggaag gttgcattca aaaagcaagg gaaggaaaat
      721 ggaatggtgg ctttgctccg tatggatata aacttgaaga cggcaagctg tttataaatg
      781 aggaagaggc agttgcaata agaacgattt tcgaccagta tgtaaatact acgataggag
      841 ccaatgggat atctaaatac ttagagaatc atggaattag aaaaatccca agacagaatg
      901 gtaagaatcc tttgtttgat gcaggtctta taagaaagat attaaagaat cctgtatata
      961 atgggaaaat agcatttgga agaagaactt tagaaaaagt tcatggtaca agaaatgaat
     1021 ataagcaggt tgaacaagat gaatatctaa tatctgaagg gatacatgaa gctatagttt
     1081 ccgatgaagt ttggcaagct gctcaggtta agctaaaatc tcaagcaaag aaatatgagc
     1141 atgtgaataa aggaaaagat acacgcacac acttgctttc tggaattgta aaatgcccga
     1201 tatgtggagt gggaatgttt gggaacaagt gtatcaagaa aaagaaagat ggcacaaagt
     1261 ataaagattt ttattactat ggttgtaaac ataggcagat gataagaggt cataagtgta
     1321 cattcagtaa gcaaattaga gaagaattgt tagatgatgc tgttgcagag gtgattgtca
     1381 agatagtaag taatccgaaa tttgcttcta tgatgcaaga aaaaatcaac atgaaggtgg
     1441 atacctctga aatagaaaaa gaaatagata attaccaaaa ggaattgaga aagagtcatt
     1501 ctacaaagtt taagctaatt gaggaaatag ataatttaga tgttgaagat aagcattata
     1561 agagaaggaa acaggattta gatgatagac tatatcgtat gtatgacaaa atagatgaat
     1621 tagaatcatc actaattgat gcgaaagcaa agaaacagac tattgaagct gaaaagctta
     1681 caggagataa catatataag gttctaatct attttgataa gctctataaa gtcatgaatg
     1741 atgtagagcg taggcagtta atttcagctt tgatttctga aattcaagtt tatgaagaaa
     1801 gacaatctaa cggacaatgg ctaaaatcca ttacttttaa gctaccaatt atagaagaaa
     1861 atctcaacat aggtttggac aatgatgaac aagttgaatg tgtaagttta ttagagaaac
     1921 gtagttaatc ctcaaaaggt tccccgaacc ttttgagtcc cactgacgca tcacactgag
     1981 gcggtatcac tgcttgtacg agttgaagca tcagcaaagt aaaagtagat gttcagccaa
     2041 tgcgacgagg tgagaccgaa ggtcgataag gtgtgttctg ggtataagac tcgtaaagta
     2101 agtaggaaat atgatgagat gatacagaga gcctgaattg gtcaggtgac gtgcttattt
     2161 acattagcct gataatgaaa atggagagat tatcgatggt tatattggaa ttgtaccaaa
     2221 atgattattc taaagattta gtagcatttg attccataga agatgggaaa gcctttgtag
     2281 ctcaaatccc gggatacact ctggaaacag aagatggttt tgaagtggag tattttaatc
     2341 ctaaaaacat accggattat atggaaatta tttttaacgg aaatattgtt cctttatcta
     2401 aatttatgtt cgatcctgag gaaaatgtaa acattatttg gaaagagatt tcaaatttat
     2461 ccctcaaaaa tgatagagtg atcgaaggat attcaaaaat tgatgcatat gtcgttaaca
     2521 accatgaagt taaagcctat gtcgaaacaa gagaaacgaa ctatcgtaaa gcaaaagact
     2581 tcttagaaag ccgcggatat gaaatcgaca gaagcttttt cggaagtgaa gacggtgaag
     2641 cgattcttta cagaaaaaaa gggatcgaag tttggcattt cttgtgtcac ttagacccaa
     2701 tgtttgtaga gattgaagat gtagaaggat atgttaaaga agaagttgga gagattcaat
     2761 agtcaaccaa agttccttgt gagtatatac ctttgctagt aaaagagtca aaacgttata
     2821 acgaaggggg ggaagaaaac ccttgagtgt aagtcttttt cttgtttaga gggaaagtat
     2881 tagagtagcc gctcataatt tatactagat ttgataactg atttgagaca gattttattt
     2941 ctataaatat t
//
LOCUS       DAVLWA010000133         2911 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLWA010000133 DAVLWA010000000
VERSION     DAVLWA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.133"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(122..655)
                     /locus_tag="VYN80_001979"
     CDS             complement(122..655)
                     /locus_tag="VYN80_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1541829.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(744..1520)
                     /gene="recX"
                     /locus_tag="VYN80_001980"
     CDS             complement(744..1520)
                     /gene="recX"
                     /locus_tag="VYN80_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1541830.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1558..>2911
                     /gene="rlmD"
                     /locus_tag="VYN80_001981"
     CDS             1558..>2911
                     /gene="rlmD"
                     /locus_tag="VYN80_001981"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1541831.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          914 a          527 c          534 g          936 t
ORIGIN      
        1 ctctttctag taatgtgttg taactctgct ataatggatt tattcctttt tgtatttaca
       61 caatttattt tatactacca aaaaaacaag gtcaggattt tgttcctgac ctttgacaac
      121 tttaccgatt ctttagttct acatagcgct tgtaccaaat gtttacatag gcttctgaga
      181 aaggaccacg tccattgtta atccaatcaa caagaatttt gacatgttct tttaaaatat
      241 agtccaagtc atcagaataa ttcattttgc gtttgtgacg ctcgtattct tcaacgtcca
      301 agagacgttt ttccccatct gtaaaaattt taacatccaa atcgtaatca atatacttca
      361 gtgcttcttc atccagatag taggggctag ccatattgca atagtaagaa gttccattat
      421 cacgaatcat ggcaatgata ttaaaccaat atttcttgtg aaagtaaaca atagccggtt
      481 ctcgagtgac ccaacgacga ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa
      541 taatggcgtt ttctgttgtt tttagtacca tggtgtcccg ccaagttcgg tggagactcc
      601 catcatgctt ataactttga attgtaataa agtcgccttc ttttggaagc ttcataacta
      661 accaactttc tacaatttat aagtttatca tttactattg taccataaaa ttacctaaaa
      721 tctgtgaatt tcacttggaa atattaaaga tattctctaa gagcgcttgc tatatccgaa
      781 aaatcgtagc cctttcgtgc taaaacttga gttaaacgct gcttcagttc gtatccttca
      841 tactttcgag catacttagt atattgctta tcaagttcct tgaagatgag ttcttgagtc
      901 gtttcttggt caacttggct atctaagtcg tcaaaggcaa ttttagcatc agaataagag
      961 aagcccttgt tagtcaagtt ctggataatc ttatcttgca gggcacgagc tggaagtttt
     1021 ccctcatatt ttttcaatag tttattggct acacgttgag caacttcaga aaaatcaaat
     1081 tctttcaagt tctcttctat agtagatttt gaaatccctt tttgagatag tttctgagtc
     1141 agcacataag gacccttgtc tcctgaaagt tgattggtat tgatgatagc ataagcgtac
     1201 tgaccatcat taatccactt atcttcttta agattagcaa taacttgaga aacgatgttt
     1261 ttatcaatat cgtatttttt cagatattct ctgacttctt tttcagtgcg tgctttaaag
     1321 gataagtggt agagagcgag attcttacca taagaaaatt gagcaaagtc ttgaatctct
     1381 ttcaattcct cttcgcttat caccttatct ctcgataaca taaaacgaac aattgtatct
     1441 tcggtgatat agcatttgtc gccattatca agctccatca ggtagagtct ttttttcttt
     1501 tcaagttttg tgattttcat agctctatta taactcaaaa tgtgataaga taggggtatg
     1561 aatctgaaag tgaaacaaaa aataccatta aaaatcaagc gcatgggaat taacggtgag
     1621 ggaatcggct tttaccaaaa aacattagtc tttgtaccag gagctctcaa aggcgaagat
     1681 atctattgtc agattacttc tattagacgc aactttgttg aagcaaaatt actgaaggtc
     1741 aacaagaagt ctaaatttcg aattgtgcca tcttgtacta tttataatga atgcggaggc
     1801 tgccaaatca tgcacctgca ttatgataag cagctggagt tcaagacgga cttacttcat
     1861 caagcgctga aaaaatttgc tcctgcagga tatgaaaatt atgaaattcg tccaactatt
     1921 ggaatgcagg aaccaaaata ttacagagct aagttacaat ttcagactcg aaaatttaaa
     1981 aatcaggtca aggcgggctt atatgcacaa aactctcact atttagtaga gttgaaagac
     2041 tgcctggtac aagataagga aacccaagtg attgctaatc gcttagcaga attacttact
     2101 tatcaccaga ttccaatcac ggatgagaga aaagttctag gtgtccgtac tattatggtc
     2161 cgacgcgcga gaaagaccgg acaggttcag attattattg ttacaaaccg ccagcttaat
     2221 ttaactcaat tggtaaaaga gttggttaaa gatttcccag aagttgtgac agtagctgtt
     2281 aatacaaata cagctaaaac cagtgagata tatggtgaaa agacagagat tatctggggg
     2341 caagagagta ttcaagaagg tgtactcaat tatgaatttt cactatcccc tcgagctttt
     2401 tatcaactaa atcctgagca aacagaagtc ctctatagcg aagcagtaaa agcgctggat
     2461 gttgataaag aagaccattt gattgacgct tattgtggag ttggaacgat tggatttgcc
     2521 tttgcaaaga aagttaaaac actcagaggt atggatatta ttccagaagc tattgaagat
     2581 gccaagcgaa atgctaaaag aatgggattt gacaatactc attatgaagc tggaacggca
     2641 gaagagatta ttcctcgttg gtacaaggaa ggctaccgag cagatgcttt gattgttgac
     2701 ccaccacgta caggtctgga tgataagtta ttagatacta ttcttactta tgtaccagaa
     2761 aaaatggttt atatttcttg taatgtttcg accttggctc gtgatttggt acgcttagta
     2821 gaagtctatg atcttcatta tatccagtcg gtcgatatgt tcccacatac agctcgaact
     2881 gaagctgttg taaaattaat aaaaaaagtt t
//
LOCUS       DAVLWA010000134         2893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLWA010000134 DAVLWA010000000
VERSION     DAVLWA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.134"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            339..476
                     /locus_tag="VYN80_001982"
     CDS             339..476
                     /locus_tag="VYN80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1541832.1"
                     /translation="MEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEK
                     N"
     gene            542..2194
                     /locus_tag="VYN80_001983"
     CDS             542..2194
                     /locus_tag="VYN80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1541833.1"
                     /translation="MLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLLIFILT
                     FVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAILMQKI
                     LSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVRGIISL
                     ILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTSPGFDE
                     QAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDYNPNGN
                     VLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFEDDLTSR
                     MSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFW
                     IDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAV
                     LGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASV
                     FLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2196..2828
                     /locus_tag="VYN80_001984"
     CDS             2196..2828
                     /locus_tag="VYN80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541834.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT          862 a          533 c          612 g          886 t
ORIGIN      
        1 gaaaaagaaa ggggtttcat tgcatgagaa aaaaacgtgg aattaaaaaa ttagttacat
       61 ttgatgcggg atggtaaaga ataattgaag aagattcttt tatcaatagt ttagtaagct
      121 tgttagttta tagagggcag gggtatttct gccctctgtt ttagaataag gaatgcacta
      181 tgaaaaaatt atttatactc atatccaacc tgcttgctag tttgttcttt gtttgggtat
      241 ttaccatttg gactgataca tatgtctccc attattaccc caacgttgtt gtacgtgatt
      301 cttcccctga aacaactttt cagcatgttg ctacacgctt ggaaaaacta gcagaagaaa
      361 cagatagctt tattgcgatt caacaccaag attctaactc agaaggtact acagtttttt
      421 cttatacgac ctttggggac ggaaagttgc ctgatgggct tcaggaaaaa aactagaaga
      481 tgctcaaagt agtagtgttg aaacaaacta ttttgtattc gatggacacc tagatattca
      541 cttgctaagg gaggagctaa gtcaacttgg cttgactaac atgaacctga caattccatc
      601 taaactatct acgttaatgg ctatctttag taacggattt cagttaatca gtctattgat
      661 tttcatctta acctttgtag cactgacttt aattagtcaa attagccaat tacggtcgtc
      721 gggcattcgc ctcatttcag gagagaaacg gtggtccatt ttccttagac cagttggtga
      781 ggatttgaaa gggatagctg ttggtttcag tttagctggc gtgctcgcca ttcttatgca
      841 aaaaattctg tcgcttccaa cgcaatccct aatgaccata ggagcaggct tgctcagtta
      901 taatctcatc ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag ttgggataaa
      961 aaagatacac ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa tcattagttt
     1021 gattttaatc ggtcaactat tagcgattat tattgtaacg ctgggaatag ggagtagctt
     1081 aaagtattcc caagcctggc aacagcatcg gattggacaa gaggcttgga gtcaggaaag
     1141 gcaactgatt accctatcaa tcagccgtga aggaacgagt cctggttttg atgaacaagc
     1201 tcaaaggaaa ctcagaactt ggtatcaatt gatggatctg gctgtttcag aacaaaaggc
     1261 tttcttatct agacaccagt taattgaccg tactttgcaa aatggcatgg cttcctccaa
     1321 aaacttgata acctctacag aatggcacga ttacaacccg aatggcaatg tccttatcgt
     1381 cacaccgcaa tacttggagc gtcaaaacat tcctgtagat acaactattg agcaaaagat
     1441 gaatcacctt aatgtagggg agtttgtctt attgctgcct gaacacctcc gttcagagga
     1501 agaacattat aaatctgttt ttgaagacga cttaaccagt cgcatgtcta gtcaagatga
     1561 acgacagcaa atgactgcta cggtaggtta tttagaatca ggtcaggatc gttttgtgta
     1621 taatacgacc cctatttctt accagcagtt tttgaaagat ccaatcatca ttgttataac
     1681 accccaatca actggtccac agtccatttt gttttggata gacgcagtac agaactacgt
     1741 tctctttaat caattgtctg atgcccagga gcttatccag agacaaggca ttgaaaattg
     1801 ggtctcagaa atgcaaacag gttaccacaa ctacatcaca ttattggata atatccagag
     1861 ggaacgttgg gtaatgctag caggagctgt gcttgggatt gcaacttcaa tcttgttgtt
     1921 taacactatg aataggctct actttgaaga atttagacgt gccattttta tcaaacgcat
     1981 tgcaggtctc aggttcttag aaatccatcg cacttatctc tttgctcaac tgggtgtgtt
     2041 tttactggga tttgttgcga gtgtatttct tcaggtagag ataggagttg ctttcttagt
     2101 cttgttactc tttactggtc tatctctttt acagttacat gtccaaatgc agaaagaaaa
     2161 caagatgtcc attcttgttt tgaagggagg ttaatatgat tgaacttaaa caggtgagta
     2221 aatcttttgg agaacgagag ttattttcga atctttcaat gacatttgag gctggaaaag
     2281 tctatgcctt aattggttca agtggtagcg gaaaaacaac cttgatgaac atgattggga
     2341 aattagaacc ttatgatggg acgatttttt accgaggtaa agacttggcc aattataaat
     2401 cgagtgattt tttccgtcac gaattgggct acctcttcca gaactttggc ttaattgaaa
     2461 accaaagtat tgaagaaaac cttaagctag gtctcattgg tcaaaagttg agtcggtcgg
     2521 aacagcggtt gaggcagaag caggctttag aacaggtcgg cctggcttat cttgacctag
     2581 ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg gcaaaaatta
     2641 tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata gacccagcaa
     2701 cctctcagtt gattatggag attttgctat ctcttcgaga tgataatagg ctaatcatta
     2761 tcgcaacaca taatccggca atttgggaga tggctgatga agtgttcacg atggatcatc
     2821 tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta tcatgaatca
     2881 agaattggag tta
//
LOCUS       DAVLWA010000135         2667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLWA010000135 DAVLWA010000000
VERSION     DAVLWA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.135"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(204..584)
                     /locus_tag="VYN80_001985"
     CDS             complement(204..584)
                     /locus_tag="VYN80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1541835.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            complement(1001..>2667)
                     /gene="phtD"
                     /locus_tag="VYN80_001986"
     CDS             complement(1001..>2667)
                     /gene="phtD"
                     /locus_tag="VYN80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1541836.1"
                     /translation="LVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIP
                     LRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDG
                     YVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKTY
                     DLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLG
                     KPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDS
                     LSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNL
                     QYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERP
                     HSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEDKEHDEVSEPTHPESDE
                     KENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEK
                     VTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESK"
BASE COUNT          738 a          555 c          479 g          895 t
ORIGIN      
        1 tttgaaggat tctaaagcta gaatagcccc atcacaatca gttttgattg attcacaatt
       61 tagaaacact atagtttcac tcctgttaaa ataaaaagga actgcataaa gcaatccctt
      121 tctaattttg aaatcattta cttaacattt tatagttgag ataatcaata gcttacctat
      181 aaaaagagtt atagtaaaat tccttattta ttgatttcaa gctccgctaa ctgtgtttga
      241 ataactgaca gttctgcacc agcctgcaga agagcagctg cattataggc accttctaca
      301 attggaaccc tgttgatgat gatactttta tcactgaaat cagtcaccat ttttaagttc
      361 attttagcag aacctaggtc aaaaaaggca agtaaagtat ctgctggatt ttcggaaaca
      421 accctatcta cttgatcaaa actcgttcca attcctccgc cctcggttcc tcctacataa
      481 gtaatcggaa catctttagc tactttacta atcagttcaa caacaccttc tgcaatgtgt
      541 ttggaatgtg aaacgataac aagaccaata ccaatacttt ccatcaaacc accccagttt
      601 ctaaaatagc agtaaagagt aatcctgatg agaatgatcc aggatcaata tgtccaagaa
      661 accatatgct cctaagacaa gagccaacag actggtcatc aataatagta ttgttctttt
      721 tttcatcatt actccttaac tagtgtttaa ctgattaatt agccagtaaa tagtttatct
      781 ttatttacac tatctgttaa gatatagtaa aataagaaca ggacagtcaa atcgatttct
      841 aacaatgttt tagaagtaga ggtatactat tctagtttca atctactata ttttgcacat
      901 tttcatgaaa aaaatgagaa ctagaactca cattctgctc tcatttttcg ttttcccgtt
      961 ctcctatcct gtttttagga gttagaaaat gctgctacct ttacttactc tcctttaata
     1021 aagccaagag actatctact tctgctgaaa tagtgttatt atctttcgtt ccgagaagaa
     1081 gactactttt tagaccagtc aatgtctcca cagcattttg tctaatacta gaatctgtta
     1141 ctttttctag caaggcctcc gcatctgcta tcttagcgtt aataacagaa tgctctactt
     1201 gaggaatttc agcctcatct gtagtatctt cagcttcttc ctctgtctct tccgtatcag
     1261 tgcttggttt ataaagatta tctgctgaag gatttaaacc agcgtgattc tctttttcat
     1321 cagattcagg gtgagttggc tcacttactt catcatgttc cttatcttcc tcaggttttt
     1381 ctgtctgagg tttctcctcg tttggttttt ccgtttgatt ggtatcagct tgaccatttt
     1441 tgtttctttg aacatggtcg ctagcgttac caaaaccatt atctgaatgc ggacgttcgt
     1501 ttggatgttc gacatagtac ttgacagtcg ccaaaagatc ctcaagagta taccccttag
     1561 gtgcctcata aaggccttcg tcaaaccact caaatttgat gttatggtaa tggtcataat
     1621 gaggtatgat taaactaccg tttttgactt ctacagtata ttgaagattg taaggcatac
     1681 gatcaagtgg caccttctta gctgctttca cgcggttgta gatagcttct gctccttttg
     1741 cctcagtatt tcctgaatcc tgatggtctg tcgaaggagg ggtcaaacct ttctctttag
     1801 cataagcctg ggctgccgct ctctcagctt cagacaaact atctttttta atccagtggc
     1861 tatgggtcat atgtggagtt acataggcat ccccctcatc actggttata tcacgaggat
     1921 caaagatata accgtcttct gttgtgtact tgcctgccaa cttggctact tgaatctcat
     1981 catcagtgta ggtaatttgc gcatttggtt ttcctaaacg ttctggatga cgaatcggag
     2041 ctaagaaggc aagaatatca tccactaact tgactttatc acttgagaca tccttgagtc
     2101 gttccaacag gttatccaaa gcctcaaaat caacttgtcg acctttatta tcaagtaaat
     2161 cttggtgaat tcttgctagt aagtcataag tcttattgta aaattctcta tcactagatg
     2221 ggaggtcagt tttcttagct cctagcttat gcgataaact ttcctgcttg gccagtttgc
     2281 tatcaatgcc tgctgctgtt tctgctgaaa gatccttggc tgggatataa cgagaaactc
     2341 cattctcctc aaagacataa ccatcgccta cttttcgaac agcttctttg accaatttct
     2401 catcaattgg attgcttgga gctggttgag gatttggtgc aggttgcaga cttggactag
     2461 gttccggagt cgattgtgga cttggttgtt ctggtcttga atctggtacc caatggtttg
     2521 aacgataacg aaggggaata atacgagcaa ttcgtttttc caattcagac atttgttcat
     2581 aagggataaa gtggtaatgg ttaccatgag ggacagctac acctctggcg gttcgacttg
     2641 tgatttgcgc tgggtcgaaa acaaggc
//
LOCUS       DAVLWA010000136         2621 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLWA010000136 DAVLWA010000000
VERSION     DAVLWA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.136"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..203)
                     /locus_tag="VYN80_001987"
     CDS             complement(<1..203)
                     /locus_tag="VYN80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1541837.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVVLHELGHKDHTRSEHQNAR"
     gene            complement(216..479)
                     /locus_tag="VYN80_001988"
     CDS             complement(216..479)
                     /locus_tag="VYN80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1541838.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(479..712)
                     /locus_tag="VYN80_001989"
     CDS             complement(479..712)
                     /locus_tag="VYN80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541839.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(712..1080)
                     /locus_tag="VYN80_001990"
     CDS             complement(712..1080)
                     /locus_tag="VYN80_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541840.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            1652..1843
                     /locus_tag="VYN80_001991"
     CDS             1652..1843
                     /locus_tag="VYN80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1541841.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            1866..2069
                     /locus_tag="VYN80_001992"
     CDS             1866..2069
                     /locus_tag="VYN80_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541842.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(2224..2391)
                     /locus_tag="VYN80_001993"
     CDS             complement(2224..2391)
                     /locus_tag="VYN80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV1541843.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            2411..2621
                     /locus_tag="VYN80_001994"
                     /pseudo
     CDS             2411..2621
                     /locus_tag="VYN80_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
BASE COUNT          758 a          443 c          575 g          845 t
ORIGIN      
        1 cgggcgttct ggtgctctga gcgtgtgtgg tctttgtgtc caagttcgtg taggacgact
       61 tggatttgtt gttcaggagt caagttgtgg tcaatagcga gcacgtttgt gtctggattg
      121 tagaaaccgc tattgtgcca gtttgaaccg tcgaagagac aaagttctac ttgatattct
      181 tcgcagagtt tagcgagtgt catagttctc cttcgttatt tatagacatc tctgcggtgg
      241 gcgatttcca cggctaggac gactagttta tcgtcttgga tatcacagat gatgcggtaa
      301 ttctctactc tgtatcgcca gtaacctgca aggttggctt ttagtgcttt tccgtgttgt
      361 cgtggattgg tcgtgttttc aatgttttta gcaagccagg ataggatttg ttttctggtt
      421 ggggtatcta gttttttaag ttgcttgaga gcttttttat caatatctag ccgatacatt
      481 aagcaatatc ctctcgagtt agtcctagtt catccaagac ctcatccatg gtataagtag
      541 ctgggtcggc taaatactcc atataggctt ggtcggctgc tcgtgcgtct tcgatatctt
      601 ccatgagtgc catgaagtcg tcaaaatcca tggtcgttgt gtcgataccg tgtttgttta
      661 ggtagtccgt aatgtaggaa tttttttctg tgaagttgat agtgatagtc attagcgttc
      721 tcctttgctt ttgaagtggg cggataggac ggatgtaatg aagtcgatat catcttcatt
      781 tagtggtttc ccgtcaaata acatggtgtt ggctgctgct ttgcgtaagt ctatgatttg
      841 tccgtttact tgggcaaatt catcactccc agcgatagca gggttatctg ttcgtccgag
      901 caggtagtcg gtggatacgt tgaaataatt agcaatttct gcgatacgtt cagcgtttgg
      961 cgtagaattt tttatcttat agagtgtatt tctaccataa cctaagtctt cttctacttg
     1021 tccaagagct tttccacgct tttttgccaa ttcttttatt ttttcaaatg tctcaaacat
     1081 tgttaaatca acctttctaa gacatcacaa aaaaaattta acaaatttgg tgtaaaaagg
     1141 ttgactaatt atcccaaaag gtgtaaaatg ttttttgtaa gtgataaaca actaaaaaaa
     1201 caactaagaa ataaattata aaaatgtttt ggcgaacggt atttatagat ttattggtgt
     1261 ttttattatg ctttaatttt agccgatttg gtgtaagttg tcaagtataa tgcagaaaaa
     1321 tagttacatt tttagttgtt tcttatttac aaataagtaa agaggaggaa cgtgctgatg
     1381 gtaagtattc ttaaaaattt agaacaagaa aaagaccacc ttgaaaaagt cattaaggta
     1441 gtcagcgctg gtggtaaatt tctgagattg ccatatcaaa aaagtcacgc tcgattagtg
     1501 agaatctgaa attgatttct caaaatcttt accatcatcg tagatgtcgc gtttgcttgc
     1561 tttgagtaaa tattctttaa aagtcataag gttaactcct ttctgcttat attatagcag
     1621 aaaaagaaga aagaaaaaga aaggagaaaa tatgccagat atcgcaaacg gtcgcgaaag
     1681 agttattgct ttcttgaaag agaaaggcat taaaaaagca actctagcgg ttgcttacgg
     1741 ctttaaacga caggaagtga caaacattct aagtggaacg actaaaggtc cacgagcgaa
     1801 cagtttcatt cttcaggtta ttgaagatta tgggattgag taggaaagat ttgaggagta
     1861 ggaaaatgag accaaaacga tatccgtata gtggacaaaa agagtccacc tttgtaaagg
     1921 cagaccctga gttagttgaa aaacttttaa gaaacactag ttttcttgag tgtttacaaa
     1981 aaaagcctat caattttcag atagactcag aagaatttaa gcgtcttagc tatgaagcca
     2041 ttcatgatac ttctcaagta actcaatagg aggaaggaat aaaatgattc accattatat
     2101 aactcactat gccagcaatg ggaaagatta cgccgaagca tggattcaaa ttgatttttt
     2161 gggaatgtgc ttttgtgtat ggaaaaagcg tacaaccatt gaacgattgt acgcaaacga
     2221 agattagact tttttccaac cgttgccttt agcagatgtc ggagggagcc gatcaccttt
     2281 tccgatagtt gcggtatgac cattagtaac ttttccgcca cgaggtccta cctctacata
     2341 gcgaccaggt ttctgattat ctgttccagg ttttattgga gtatttgcca tactatctcc
     2401 tcctttctat aagtttgaga aaatcaacgg tatctggtat tatttcgatg gctcaggcta
     2461 tatgctttca gaccgctgga agaagcacac agacggtaat tggtactact ttgaccaatc
     2521 aggcgaaatg gccacaggct ggaagaaaat cgctgacaag tggtactatt ttgatgtaga
     2581 aggtgccatg aagacaggct gggtcaagta caaggacact t
//
LOCUS       DAVLWA010000137         2584 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLWA010000137 DAVLWA010000000
VERSION     DAVLWA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.137"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            129..1622
                     /locus_tag="VYN80_001995"
     CDS             129..1622
                     /locus_tag="VYN80_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1541844.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2584
                     /locus_tag="VYN80_001996"
     CDS             1635..>2584
                     /locus_tag="VYN80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541845.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAY
                     GGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLV
                     FLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLAL"
BASE COUNT          912 a          387 c          503 g          782 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatttaacg
     2161 agatgtttgg ggatccttta aataataaca agaaatttgc agtaaaaacg ggacaacagt
     2221 gttttaaatt ttcaagtgga aaatttcttg ataaacatga tagagttttt gaaggttatc
     2281 ctgcctatgg tggaaatggc attgcttgga aaagtcgaaa atatttaatt gataatccta
     2341 ctatcatcat tggtcgtgta ggtgcatatt gtggtaatgt aagaactacc cacggtaagg
     2401 tatggataag tgacaatgct atatatataa aagaatttaa gaattccgat ttcaatcttg
     2461 tatttttatt agaattaatg aaagtaattg attttagtaa atttgctgat ttctcaggtc
     2521 aaccaaagat aactcaaaaa ccattagaaa atcagaaata tatcctcccc cctctagccc
     2581 tcca
//
LOCUS       DAVLWA010000138         2497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLWA010000138 DAVLWA010000000
VERSION     DAVLWA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.138"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            23..1450
                     /gene="galT"
                     /locus_tag="VYN80_001997"
     CDS             23..1450
                     /gene="galT"
                     /locus_tag="VYN80_001997"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1541846.1"
                     /translation="MYNEIDKIYVKNRVLALIGEEGTDRISDENDLKQIKDYLVEIAL
                     KNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISPQEAVEEFYKLSKDSDY
                     IKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKLVKATNYPKCLLCMENE
                     GYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIFLNSQHVPMAITSKTFE
                     QLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPMEKAKFESEFRFKDFED
                     VNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEVDIIAMTEDVPHHTVTP
                     IARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKENIGLIEVMGLAILPPRL
                     KPELEEVGKYLLGEENAIADYHLEWADQLKEKYPRINKEEVNNVVQHEAGQVFARVLE
                     DAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1463..2473
                     /gene="galE"
                     /locus_tag="VYN80_001998"
     CDS             1463..2473
                     /gene="galE"
                     /locus_tag="VYN80_001998"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1541847.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
BASE COUNT          877 a          354 c          528 g          738 t
ORIGIN      
        1 tgatagagta attgaatacg gaatgtataa tgaaattgac aaaatatatg tgaaaaatag
       61 agtactagcg ttaataggtg aggagggaac tgatcgcatt tccgatgaaa atgacctgaa
      121 acaaataaaa gattacttag tggaaatcgc cttaaaaaac ggtaaaatta aagatttaat
      181 cgaggaaaag gaatgtttag gagcagagtt aatgaatttc atagttcctt tacctagtcg
      241 attaaatgat attttttgga gttcatatga tatatcacct caagaagcag ttgaagagtt
      301 ttataagtta agtaaagata gtgattatat taaaacttct gctattgcta agaatattga
      361 gtttcgagca tcaactaaat acggagagct agagattacg ataaatttgt caaaacccga
      421 aaaagatcca aaaacaattg ctgctgaaaa attagtaaaa gccactaatt atcccaaatg
      481 tttactatgt atggaaaatg aaggatatca ggggcgaata aattatccag cacgttcgaa
      541 tcatagaatt attcgattga aacttggtga cgaagtatgg ggattccaat attctcccta
      601 cttatacttt aatgagcatg cgatattttt aaatagtcaa catgtaccaa tggctattac
      661 ctctaaaaca tttgaacaat tattggagat tgttgatatt ttgccaggat attttgctgg
      721 ttcaaattct gatcttccta tctcaggagg ctccattctt agtcataacc actaccaggg
      781 agggaaacat attttcccaa tggaaaaagc aaaatttgag agtgaatttc gttttaaaga
      841 ctttgaggat gttaatgctg gtattgtaaa atggccaatg tcagtaatta ggttacaaag
      901 tgaaaataaa aatcgcttgt tagatttagc tactaaaatt ctaaataagt ggagagaata
      961 ttcagattta gaagtagaca tcattgctat gactgaagat gtcccacacc atacggtgac
     1021 tcctattgct cgtaaagtgg acgggagata tgaattagat attgttctaa gagataatca
     1081 tactactgaa cagtacccag atggtgtttt ccatcctcat caagatgtac aacatattaa
     1141 aaaggaaaat atcgggttaa tcgaagtgat gggacttgcg atattaccac ctcgtttgaa
     1201 accagagtta gaagaagtgg gaaaatatct tttaggagag gaaaacgcaa ttgcggatta
     1261 tcatttagaa tgggccgacc aattgaaaga aaaatatcct cgcattaata aagaagaggt
     1321 taataacgta gttcaacacg aagcaggaca agtgtttgcg agagttctgg aagatgcagg
     1381 agtttataag aatacaccgt ctgggcatga agcgtttatg agatttgtta agtcggttgg
     1441 aattaattag gaggaaaccc aaatggcaat attggtaaca ggcggagctg gttatattgg
     1501 tagccatacc gtagtagaat tactaaattt aggaaaggaa gtcatcattg tcgacaacct
     1561 ttcgaactct agcatcttgg tgctagaccg tattgaagca attacaggaa tacgtcccgt
     1621 gttttacgaa ttagatgttt gtgataaaca agcattgaga aaggtatttg aacaagaatc
     1681 gattgatgct gcaattcatt ttgcaggtta taaagctgtc ggagaatccg tgcaaaagcc
     1741 tgtgatgtac tacaaaaata atattatgag tacattggca cttgttgaag tgatgtcaga
     1801 atttaatgtt aaaaagattg tcttttcttc aagtgcgact gtatatggaa ttaacaatca
     1861 gtcacctcta attgagacga tgcaaacaag tgcgacaaat ccttatgggt atacgaaagt
     1921 gatgcttgag caaattttaa aagatgttca tgtggcagat tcagaatgga gtattgcgtt
     1981 gcttcgttat ttcaatccaa ttggtgctca tgagtctggt ttaattgggg aagatccctc
     2041 aggaattcct aacaacttga tgccttatat tgcacaagta gcggtaggta agctgtcaga
     2101 gctaagtgtg ttcggaaatg attatgatac gcttgatggt actggtgtgc gtgattatat
     2161 ccatgtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat ctgaaaaaac
     2221 agatgtttat atctataacc taggctcggg agaaggcact agtgtattac aacttgtaaa
     2281 tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa gacgttcagg
     2341 agacgttgca acttgttatg caaatgcaga caaagcatat aaggaattaa attggaggac
     2401 aacaaaatcg attgaagaca tgtgtagaga tacatggaat tggcaatcaa aaaatcccaa
     2461 tggctataat tgatgatact gtttatatga gtgctgt
//
LOCUS       DAVLWA010000139         2480 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLWA010000139 DAVLWA010000000
VERSION     DAVLWA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.139"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(98..421)
                     /gene="tnpB"
                     /locus_tag="VYN80_001999"
     CDS             complement(98..421)
                     /gene="tnpB"
                     /locus_tag="VYN80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1541848.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(682..981)
                     /locus_tag="VYN80_002000"
     CDS             complement(682..981)
                     /locus_tag="VYN80_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1541849.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1146..1553)
                     /locus_tag="VYN80_002001"
     CDS             complement(1146..1553)
                     /locus_tag="VYN80_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1541850.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            1679..2480
                     /locus_tag="VYN80_002002"
                     /pseudo
     CDS             1679..2480
                     /locus_tag="VYN80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          771 a          440 c          476 g          793 t
ORIGIN      
        1 taaaatgaaa caagaacagg acaaattgat caggacagtc aaatcgattt ctaacaatgt
       61 tttagaagta ggggtgtact attctagttt aaatccacta tatttgggga gtgatagaaa
      121 agcccttcat aagccagtct acttgttcag gtgcgagagc tttgacatcc ttttctgtac
      181 tgggccaagt cagttttccg ttctcaaagc gtttatatag tatccaaaat ccttgaccat
      241 cccagtaaag ggctttaaag cggtctttac gtccaccaca aaagagaaag acttgaccgg
      301 agaaaggatc caattcaaag tgggttttaa cgagataagc cagtgaatcg attccttgta
      361 tcatatctgt cttgccacaa acaaggtgaa cttgacctaa atcacttagt tgaatcatca
      421 tagtacaata cttttcctcc gataattatt ttttatctag tatactggaa gttggggaat
      481 taggatagat accttgttat gacgcgctta cgtaacttgt aactagctgc ctagtttgat
      541 ctttgcttct tcattgatta gcagcagatt tcaaaatgat aaaaacgtat aatagtgggt
      601 gtagaaactc ttgctactat gcgttttatt atggaaagac ttattggact ttctctcaaa
      661 tcgagttttt actcaatttt cttacttgat tgggattgaa attccaatta atttctctga
      721 gtagagtgtc ttgatattgg cttcatcaac agaggcctta tcaattttac gtttcaagaa
      781 aaattcttga atggtttcga tttcaggctc acgaatagca cggtgtttgt ttgagatgag
      841 gatttcatag tgaagcggag cttgggtaaa aataacatct gtattccctg cagaataaac
      901 ctcaacaagg gttgcatcgg tactttctag ctgacttttt acaagttgcg agtgtgagtt
      961 tgtcgtattg ataagcttca taatatttcc tccgattttc taattctatt atagcacttt
     1021 ttgaataaag tcgcttgatt tatactcaat gaaaataaaa gagcaaacta ggaagctagc
     1081 cgcaggctat acttgagtat ggtaaggcga cgctgatgtg gtttgaattt tattttcgaa
     1141 gagtattagt caatcttatg ctgttttttc caagattcaa tggcccattt atggctacca
     1201 cgtttaaggt ttttgatagc ctcgtcaata gggaaccagg caatatgatt aaagttttct
     1261 agtggctttt gtacttcttt gaaaggagtt gcttcataga ggtaggcagg attgtagtag
     1321 taggtatcac gatgacgaga atagaaatat tcgtcagctt gtccgtaata ggtaccaatt
     1381 tctgctgtga aaccaagctc ttcaatcaac tcacgcttta gggcttcctg atgattttca
     1441 cctgcttcaa tttctccaca tggtaggaac caagcaccat ttggttcttg aacaagaaca
     1501 atttgttttt gttcaggatt agggataact gcatatacgc catagcgagc aatatagtct
     1561 gtattcgctt tttttctccg aaagttgggt ttgccattgc attttcctca ttatctagta
     1621 tcgttattat tatagtgaaa tgaaccaaaa atagtacaca atgtggtata atcttcttat
     1681 ggcatattca atagattttc gtaaaaaagt tctctcttat tgtgagcgaa caggtagtat
     1741 aacagaagca tcacacgttt tccaaatctc acgtaatacc atttatggct ggttaaagct
     1801 aaaagagaaa acaggagagc taaaccacca agtaaaagga acaaaaccaa gaaaagttga
     1861 tagagataga cttaaaaact atcttactga caatccagac gcttatttga ctgaaatagc
     1921 ttctgaattt ggctgtcatc taactaccat ccactatgcg ctcaaagcta tgggttacac
     1981 tcgaaaaaaa gaaccacacc tactatgaac aagacccaga aaaagtagcc ttatttctta
     2041 agaattttaa tagtttaaag cacctagcac ctgtttagat tgacgaaaca ggattcgata
     2101 cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaataaga ggcaaagtat
     2161 ctggaagaag atatcagaga atttctttgg ttgcaggtct aacaaatggt gagttaatcg
     2221 ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagcttgg tttcagaagt
     2281 ttctcttacc aacattaacc acaccatcgg ttattattat ggataatgca agattccata
     2341 gaatgggtaa gttagaactc ttgtgtgaag agtttgggta taacttttta cctcttcctc
     2401 cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa aagcacctca
     2461 aaaaggtatt accaagttgc
//
LOCUS       DAVLWA010000140         2440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLWA010000140 DAVLWA010000000
VERSION     DAVLWA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.140"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(112..408)
                     /locus_tag="VYN80_002003"
     CDS             complement(112..408)
                     /locus_tag="VYN80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV1541851.1"
                     /translation="MKSKRMEFHNKFLIVSAILAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(939..1832)
                     /locus_tag="VYN80_002004"
                     /pseudo
     CDS             complement(939..1832)
                     /locus_tag="VYN80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
BASE COUNT          843 a          482 c          267 g          848 t
ORIGIN      
        1 aaaatagatt gacaaactta tgataacaaa aaacaaaagg gttatcccaa taaaattaag
       61 ataacctaaa atactttcac attctttttt agaaagtatt ttcatctagt tctaccatag
      121 agctgcttta gcagcacctt tctttttcaa tatataaaga actgttgctg caatacccga
      181 accaatagaa cctactcctg tgattgcacc tacaattgaa attactgctg taactgtact
      241 ataagcacta atcaaattga ctacagtagc agcagctttt gtcgaaattc ctaatgtccc
      301 agccaacata ggaaaagaaa caacacctaa gcttaaccaa ctaatgactg ctaatattgc
      361 tgaaacaata agaaatttat tatgaaattc cattctttta gatttcattt tagtgtactc
      421 cttttgtata ttatgtatgt tttattgaga gttaattcgc acatgttgta ttcctaactc
      481 tcttcaaatg ttcgtctata ttttatacta cttttattaa catatctata ataaataaat
      541 tactactaca ttttcctaga ttatcaaatt acttttatat cactaagact caataaaaga
      601 taatgtctat ttaactttta tacgattact accttctttt tagtttacac ttcctacatc
      661 ataaataaac atactcaacc ctcacaatct ccaaataact cttctatatc attgaaaatt
      721 gagtttcatc ctctatacta atgtaggaca ttcctttaat ccaatataag aataccttgt
      781 tccattaatc tctacccgat attatagata gaaccaaatt cctatactta aaaggacaaa
      841 taacgttcca accataataa acctcctttc tttttgagaa cgttttcttt ataggtttag
      901 tatagcaaaa acatgaaaaa aacaacatga cttagaagtc aggttgttgg gaaatctatc
      961 tgtttttctg ctaaaattct ctgaactaat acctgatcgt ctagcaattc tgctagagaa
     1021 atcgcctttt catagaaact ttctgcctct aaaatattct tgtctacaaa taaagcatat
     1081 tttgccttta gcaaaaatag atgaggtcgg taaaaaacat gtcttgtttt agcaataatt
     1141 ttctcaaatg tttgaatata tatcagacta tcttctgtct gaatttggat aaataggctc
     1201 agaagaactt tttccatttg aactaggcta gccttatctt catattctga acatagcaat
     1261 actttttgag ctaactcctc aaaatgttct ttatcttcta atcccatagc acaacaaaag
     1321 aaatacaatt caataatcaa caaatcattt acagaaaaat atttcttttt taataactgc
     1381 tggaaatatt cttccactaa ccctaaaccg taggtcacat ctgaactacc ataaatatca
     1441 aaacgagctt gtgtcacatc cacaattaac tgctcttcct caggcaaatt ctcataatag
     1501 ttttcatata tttcctcaag tatagcttct tttcattcga ttcttccctt atcagcgtag
     1561 gtttgagaat gaatcagctc atctttgagt tttaggaatc cttttggaat ctctataacc
     1621 ttaacatcca caatattctg taatgatact ccaagtttat ccgcaatcaa taataattta
     1681 ggaagtgttg ctaaagcttg cccattttca atacgtgcta gctgacgaat cgtcaactct
     1741 gattcatcac cacaaatatc tatctgagtt tttccacaag atcgcctaat agttttgatt
     1801 ttataaccaa tttcacgttt caactcatcc atagtaatac tccttaagtt gattacaaag
     1861 atttcttttt cttcctaatt cttttgtcaa tcatatataa aacgataagc gaaaacagcg
     1921 gtagaacaat ataactcttt tcactcatta tcagctgaaa tcccagggtt gctagtatct
     1981 taaagatgaa aatattcatc aaaaatagaa aaacaaatac taaaaaatat tttaaaacct
     2041 tcttcatttc ttttcttcaa ctttcctaat ttctacacct attttatcga aaatatatat
     2101 aaaactccaa ataaacactt caactgcact ttttttggca aaaaatagac agaacaaaca
     2161 tcctacctac taaaaatatg atatgaaagg aaaatgattt tttactgaca ccctaggcaa
     2221 gcttgggaca aataaatcat tgatagcaac atttacgaca tcctatttaa agttccttca
     2281 atttctaatg tccccgctat ttctaagcca cctctggtat gatctattcc tagtcagaat
     2341 agataacacc gttctaaatt acgattcata gttactcctt ttttgactcg ttactataag
     2401 actcctattt acatgcgcaa aataattttg attcagattc
//
LOCUS       DAVLWA010000141         2205 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLWA010000141 DAVLWA010000000
VERSION     DAVLWA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.141"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..764
                     /locus_tag="VYN80_002005"
     CDS             <1..764
                     /locus_tag="VYN80_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEV1541852.1"
                     /translation="KKKFRHISLTGDELKYKMEFSNKVWICWFQGEEHAPELIKTCIQ
                     SMRNQLQGREIIVLTEKNISDYTEIPDYIIEKYKKGWISRAHYSDILRIDLLCRHGGL
                     WVDATVLNTGGDFSNLEVPLFVYKSLNLSRKDSQAIAASSWLISSYSNHPILLYTRKL
                     LWAYWRRKNSLCNYFLFHICFTIATERYPIEWSAVPTFNNHSPHILHFELNEQFSEKR
                     WEQLKHISAFHKLNHHIDYSSGVNTFYKFIVSSKV"
     gene            769..>2205
                     /locus_tag="VYN80_002006"
     CDS             769..>2205
                     /locus_tag="VYN80_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEV1541853.1"
                     /translation="MSNKISKNLAYNMGYQLIGIAAPLITSPYLSRILGAENLGIHSF
                     TMSVALYFMMFMLLGIANYGNRTIATVKREGKDILSKTFWSIYSIQLIMSILVTIAYL
                     AYLYLGAVHYKVIAILQLFLLLSNAVDITWFFYGLENFKQIVFRNTLVKLLGLFLIFL
                     CVRQGTDLWKYTFINGSVTLVGQLLLWGQLKGILTWKKIQLKELLPHIKPILILFIPV
                     LAISIFTNMDKYMLGLMVDVKQVGFYDNAGRIIEIPKALIAALGAVMLPRTSYLLAEG
                     QKEKSKYYIEVTILYVMIISSVLMFGLISVSDIFSIIFWGEEFLESGRLISAMSPAFV
                     FSVLGNIIRTQYLIPRAKDKDYVVSLIAGAVVNLVLNYFFIKPFGAMGATVSTVLAEF
                     VLSGMQFWSVRRDLDLKRYLKNGLIFYLFGLIMHLIIIALKTQLPYNIMSLILLIVLG
                     GIVYVIFCCCYILMSRNLHFKLLKEKIIR"
BASE COUNT          710 a          288 c          404 g          803 t
ORIGIN      
        1 taaagaagaa gtttagacat atttcgttga ctggagatga gcttaaatat aaaatggagt
       61 tttcaaataa agtttggatt tgttggtttc agggagaaga gcatgcccca gagttaatta
      121 aaacatgtat tcaatcaatg aggaatcaac ttcaaggaag agaaatcatt gtgttgacag
      181 aaaaaaatat aagtgattat acagagattc cagactatat tattgaaaaa tacaaaaaag
      241 gatggatttc tcgtgcgcat tactctgata tccttaggat agatcttcta tgtcgccatg
      301 gaggattatg ggtagatgca acagtgttga atacaggagg agatttctca aatcttgagg
      361 taccactttt tgtatataaa tctttgaatt tatcaagaaa agattcacaa gctattgcag
      421 catcaagttg gttaatttcg tcatatagta atcatcctat attactctat acgagaaaac
      481 tcctatgggc ctactggcga agaaaaaata gtttatgtaa ttattttttg ttccatatat
      541 gttttacaat agctacagag cggtacccaa tcgaatggtc tgctgtccct acttttaata
      601 atcattctcc tcatatactt cattttgagt tgaatgaaca attttcggaa aaacggtggg
      661 aacaactgaa gcatatttcg gcgtttcata aattaaatca tcatatagat tattctagcg
      721 gtgttaacac tttttataag ttcatagtat cttcgaaagt ttagaaaaat gagtaataaa
      781 attagtaaaa atttagccta caatatgggg tatcagttaa ttggtattgc agctcccttg
      841 ataacttctc cttatttatc acggatatta ggtgctgaaa atttaggtat tcattctttt
      901 actatgtcgg ttgcacttta ctttatgatg tttatgcttc ttggaattgc aaattacgga
      961 aatagaacaa ttgcgactgt taaacgagaa ggaaaggata ttctatcaaa aacgttttgg
     1021 agtatctact ctatccagtt gataatgtct attctggtaa ctatagctta tcttgcttat
     1081 ctatatttgg gggcagtaca ttacaaagtt attgcaatat tacaactctt tttattgtta
     1141 tcaaatgctg ttgatattac ttggtttttc tatggattag aaaattttaa gcaaatcgtt
     1201 tttaggaata cattagtcaa acttttagga ttatttttaa tttttttatg tgtgcgtcaa
     1261 ggcacagatt tatggaaata cacatttata aacggatcag taactttagt tggccaactt
     1321 ttactatggg gacaattaaa aggaatatta acttggaaaa aaattcaatt aaaggaatta
     1381 ctacctcata tcaagccaat tttaattctt tttattcctg tactagccat tagtattttt
     1441 actaatatgg ataaatatat gcttggttta atggttgatg taaagcaggt tggattttat
     1501 gacaatgcag gtagaattat tgaaattcct aaagctttga ttgcagcttt aggggccgtt
     1561 atgttaccaa gaacttccta tttattagcg gaaggacaga aagaaaagag taaatactac
     1621 atagaggtaa caattttata cgtgatgatt atttctagtg tattgatgtt tgggctaata
     1681 tcggtatctg atattttttc cattatattt tggggagaag aatttcttga atcaggtaga
     1741 ttgatttctg ctatgtctcc ggcttttgtc ttctctgtat taggaaatat cattaggact
     1801 cagtatttga ttcctagagc taaggataaa gattatgtag tgtctctaat tgcgggggct
     1861 gttgttaatc tagtgctaaa ctattttttt ataaaacctt ttggtgctat gggagcaaca
     1921 gttagtacag ttttggcaga atttgtattg tctggaatgc agttttggtc agtaagaaga
     1981 gacttagatt taaaaagata tttgaaaaac ggtttgatat tctacctatt tggtctgatt
     2041 atgcacctca ttattatagc actcaaaact cagttaccat acaatataat gagtcttatt
     2101 ttactaatag ttttgggtgg aatagtatat gttatttttt gttgttgcta tattttaatg
     2161 tcaagaaatt tacattttaa gttattgaag gaaaagatta tacga
//
LOCUS       DAVLWA010000142         2201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLWA010000142 DAVLWA010000000
VERSION     DAVLWA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.142"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..2036)
                     /locus_tag="VYN80_002007"
     CDS             complement(<1..2036)
                     /locus_tag="VYN80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEV1541854.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSN
                     GYSIYNTGTSIQTMLTLGSGLGTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGN
                     GAQMNGWQAKREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASASTSASASASTSAS
                     ASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASVS
                     ASTSASASASTSASASASTSASESASTSASESASTSASASASTSASASASTSASASAS
                     TSASESASTSASASASTSASASASTSASESASTSASASASTSASESASTSASASASTS
                     ASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASAST"
     gene            complement(2034..2147)
                     /locus_tag="VYN80_002008"
                     /pseudo
     CDS             complement(2034..2147)
                     /locus_tag="VYN80_002008"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          542 a          483 c          524 g          652 t
ORIGIN      
        1 gttgatgctg aagccgacgc actggtactt gctgaagctg atgcactggt tgatgctgaa
       61 gctgaagcac tcgttgatgc tgaagccgag gcactggttg atgctgactc agacgcactc
      121 gttgacgctg aagctgacgc actcgttgag gctgaagccg aagcactcgt tgatgctgag
      181 gctgacgcac tggttgatgc agattcagaa gctgatgtgc ttgctgaggc tgacgcactc
      241 gttgatgctg actcagacgc actcgttgac gctgaagccg aagcacttgt tgatgcggaa
      301 gctgacgcac tggtacttgc tgactctgat gcactggttg acgctgaagc cgaagcactc
      361 gttgatgctg aagctgatgc actagtactt gctgaggctg acgcactcgt tgatgcagat
      421 tcagacgcac tggttgatgc agattcagac gctgaggtac ttgctgaggc tgacgcactc
      481 gttgaggctg aagctgaagc actggtgctt gctgagactg atgcgctggt acttgctgaa
      541 gctgaggcac tggttgatgc ggaagctgag gcacttgtcg atgctgaagc cgaagcactc
      601 gttgacgctg aagccgacgc actggtgctt gctgaagctg aagcactcgt tgatgcagat
      661 tcagacgctg atgtgcttgc tgaggctgaa gcactggttg acgctgaagc cgacgcactg
      721 gttgaagccg acgcactggt gcttgctgaa gctgaggcgc ttgtgcttgc tgaggctgag
      781 gcactggttg atgctgaggc tgaggcgctt tgactagcac ttactgagag tgacttagac
      841 tgtgaaagtg actgactggt cgtgctagat tcaactacct ttcctctacc attgaagtag
      901 ttagttctaa tgttatctgt tttagcagcg taaggggtaa atgtaaagga tgtatccgtt
      961 ccagtaattg gtacagtcca agatgatgtt aatccatatt ctctcttagc ctgccaacca
     1021 ttcatttggg caccatttcc ccaagtatac ctactacccc gcttcgtcat tgtagatgta
     1081 ttatagtatt gaacctgtcc accatttttg ttagtaatgg agttctttac atctgaaggg
     1141 gtaccaagac cactgccaag ggttaacatt gtttgtattg aagttcctgt attatagata
     1201 gaatatccat tactcatctt ttttgatata ttaccaagag tctttgtttt aggatccaca
     1261 tacgtaaccg tataggtaaa ggtcagtttt aagccatcat ttgtcactgt caatcgatag
     1321 taaattggaa cattatatac cgaatctaca cctttacctg tataaacttt tgtctcactt
     1381 ttagcaatgt ttagacttgc attaattgca ggggcgccat ttacaatcgt attgccagaa
     1441 aaaacagcag cttttataga agccagcaat tgctcgatgg aatccactga acgcttgcgt
     1501 cgcttagcat aagattggag attgacattt gtaactgatg ctacataatc actagctggt
     1561 ttcttacgat cttcttcgac cttcttagca gttgcttctg tagcggcagc tgtttgtgat
     1621 cctaccacag tagatgatgc agagatactt gtcgaagccg atgtacttgc tgattctgat
     1681 gcacttgtac tcgctgaggt cgaagcgctg gttgaggctg actcagatgc agatgtactt
     1741 gccgactctg aagctgacaa actagttgaa ctcgctgaat tacttgtaga tatcgtacct
     1801 agaactactg taccatttgt tgccaaagca tccgttttct ctacagtttt ttcaagtact
     1861 gccgactcat ttgtaaatac cttcgtttga gttgcaacgg ttccacttat gacagttccc
     1921 gcagcaacta tccccttgag gatatcaagc ccagtaattg aatgacttac tttatcttct
     1981 accgtttcgg tcatgacctg agtagtatca acaccaccac gcatgacctt aaacaagcca
     2041 aaaagagaag tcgaggcccg tagccaatgc ttccctgatt tgattaattt gaaccgagtt
     2101 actcggtctg tttctctata ttcacccttc tgacgtctaa aaaatatttt gaaatttcct
     2161 ttcgagtatt attttcatgt atggatataa ataaattacc a
//
LOCUS       DAVLWA010000143         2185 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLWA010000143 DAVLWA010000000
VERSION     DAVLWA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.143"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1068
                     /locus_tag="VYN80_002009"
     CDS             <1..1068
                     /locus_tag="VYN80_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1541855.1"
                     /translation="ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANG
                     FPDKVTDMDGNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKS
                     VVFNINDKRNNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTV
                     KEFILNKDTGEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGW
                     EISGFEGKKDAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQL
                     NESHRKEDLQREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTL
                     LAAGIMFIVGIFLGLKKKNQD"
     gene            1700..2176
                     /locus_tag="VYN80_002010"
     CDS             1700..2176
                     /locus_tag="VYN80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1541856.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
BASE COUNT          828 a          295 c          407 g          655 t
ORIGIN      
        1 gaatcggttg tagataatta tttaatctac ggagatttac acattgataa cactagagat
       61 tttaatatta agctgaatgt taaagacggt gacatcatgg actggggaat gaaagactat
      121 aaagcaaacg gatttccaga taaggtaaca gatatggatg gaaatgttta tcttcaaact
      181 ggctatagcg atttgaatgc taaagcagtt ggagtccact atcagttttt atatgataat
      241 gttaaacccg aagtaaacat tgatcctaag ggaaatacta gtatcgaata tgctgatgga
      301 aaatctgtag tctttaacat caatgataaa agaaataatg gattcgatgg tgagattcaa
      361 gaacaacata tttatgtaaa tggaaaagaa tatacatcat ttgatgatat taaacaaata
      421 acagacaaga cactaaacat taagattgtt gtaaaagatt ttgcaagaaa tacaaccgta
      481 aaagaattca ttttaaataa agatacggga gaggtaagtg aattaaaacc tcatagggta
      541 actgtgacca ttcaaaatgg aaaagaaatg agttcaacga tagtgtcgga agaagatttt
      601 attttacctg tttataaggg tgaattagaa aaaggatacc aatttgatgg ttgggaaatt
      661 tctggtttcg aaggtaaaaa agacgctggc tatgttatta atctatcaaa agataccttt
      721 ataaaacctg tattcaagaa aatagaggag aaaaaggagg aagaaaataa acctactttt
      781 gatgtatcga aaaagaaaga taacccacaa gtaaaccata gtcaattaaa tgaaagtcac
      841 agaaaagagg atttacaaag agaagatcat tcacaaaaat ctgattcaac taaggatgtt
      901 acagctacag ttcttgataa aaacaatatc agtagtaaat caactactaa caatcctaat
      961 aagttgccaa aaactggaac agcaagcgga gcccagacac tattagctgc cggaataatg
     1021 tttatagtag gaatttttct tggattgaag aaaaaaaatc aagattaaga caaaagctat
     1081 agaaaaaaat ggtttatgta ctgagattag atagtgaggt gatgacatag ttttgtgaaa
     1141 acggccattt ataactcaat tatttagttt acttcacttc actattgata ctatttggag
     1201 ttattaatgg acttagttta gataactaat gaattgattg aaagggttag tattgacaat
     1261 attggtcata ttgattagaa aatagagtct atcaaaattt aaaggctaat agaggtgatg
     1321 agacaatttc aaggaattaa gtagcgtttg gttgcttaat tcctttttat ttggaaggaa
     1381 gtttttcgta accgcccaat aacgaagtct attggacggt tacgatatga tcactgactt
     1441 cgtcagtctt atctacaacc tcaaaactgt gttttgagca acctgcggct agcttcctag
     1501 tttactcttt gattttcatt gagtattaga acagaaaaaa tgcttggagt atttgtttgt
     1561 gtgtttattt ttatataaca aactataaac aaaacaaaaa tataaaaaaa gagacaaaaa
     1621 agaacaaaaa gtaattgaca acggtttcat atagtgttat acttatggca taaagttaat
     1681 ggaaagaagg gaaaaactta tggtattaga ttatttcttt gacaaaaacc ttgtgttttg
     1741 cttagaagcg gataatcaag aacatctctt tgatcaggtt gcaactttat tggaagaacg
     1801 agaaattgta actccaactt atcgtgaagc cttgatcacg cgtgaaaagt catttccaac
     1861 tggtttagat atggaatttc taggaaagga cttgccaaat gtagcgattc ctcacacaga
     1921 tattgttcat aatctagctg agaaagtggt ggttgttcga ttagaaaaac cagtaacttt
     1981 tcacaatatg atagctcctg ataaggaagt agaagtatct ctactcttct ttatcattaa
     2041 caattcaagt tcaagtcaaa cgaacattct ggctcagttg atggactttt tcacaggaaa
     2101 tggtcatctt gaagacctat caaaaatttc tgaaccagaa aaactttatg cttacattgc
     2161 tgaagcaacc gcttaatctt gtcta
//
LOCUS       DAVLWA010000144         2093 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLWA010000144 DAVLWA010000000
VERSION     DAVLWA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.144"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..258
                     /locus_tag="VYN80_002011"
     CDS             <1..258
                     /locus_tag="VYN80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541857.1"
                     /translation="TLIFLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYL
                     FLGYLLVIQKAGYLFSILLLFSNFSVPFIKEKEYELFKNSK"
     gene            1384..1887
                     /locus_tag="VYN80_002012"
     CDS             1384..1887
                     /locus_tag="VYN80_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541858.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKLTSYC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTN
                     VKEMKWL"
BASE COUNT          705 a          272 c          378 g          738 t
ORIGIN      
        1 actcttatct ttttgataaa aatttgtgca gataccacga taaaattttt attcatcaga
       61 ttatttgaca caagtacttt aatgaagagg agaatatttt ttcttttcgc attggatgtt
      121 atagcttact tatttttagg atatctttta gtgattcaaa aagcaggata tctgttttcg
      181 attttacttc ttttctctaa tttttcagtt ccatttatca aagaaaagga atacgaatta
      241 tttaaaaata gcaagtaata gaaatatgga ttaaatctta gaacggctta aaatcagaca
      301 tgataaagga aagctatcta ggtaaccaag tactataaac acagtcgagg atcacttgtt
      361 aggattacat tttaatcatg cttaataaga atggagaatt cctatgggga ttgtattact
      421 ctgaagtttt gaatacctta gttctcgtga ttttgagcta ttctagcttt agaatccttc
      481 agaacgtaaa attttaagca gtcaaagact tagaatagcc cgaaaacatt tattttatag
      541 caattctaag attagaattg ctcatatata tgtatttgag ctgctcgata gttaggacag
      601 ctctattctg agaaatctga cctatcaatg cttcagaata gtatttcgca aatggttttt
      661 attgaagagg tgattgtttt atgatagtct aaaataaaac tgatttatgg agtaaatgga
      721 gaatcgtcac atgaaacatt tttttgcagg aattggtgag ataaaacttg ttggctactt
      781 attgtatata tgtgtaggac tagcccctct ttttcatata tatgtcatag gttcggaact
      841 gagttttgtg aaaatagtgt tatctatttt gggagttata tttgtaagta tgttaactat
      901 tgctcgaatt tatcggactt ttctataaag aatagtttgg acttgaactg tatctaaaaa
      961 cagaggttgg tcgattatca gcctctttct tgtcgttgaa aagagtgaga tatattgact
     1021 tttgaaaaaa atgtcataat tgagttggga taaacctaaa agtaatatag tagattgaat
     1081 ctggaatagt ccgttatgac tgttaaaata tttctagaaa ttaatttgac tttcctaatc
     1141 gatttgttca catcttgttt caatctacta tactttctcg ttaaaccgtt cggcaattca
     1201 tggtgacatg gacacatagt agtaagcaca ctatgtggct tggcagagct aaaaactgtt
     1261 cccttgcgat aagcctaata agcacaacat agggagttag agaagccgac tctaatcaat
     1321 cactttgggc agtagtgaga actgccccgt gctttttatt ttgagaaaat atggagtttg
     1381 tcgttgaaat tacttgattg tattttggat tatcaagaaa gattcaatgg aaaaacatgt
     1441 caagtatcaa cgaattataa gtatttagag actttcaaag tgaatttttg cttgactgat
     1501 ttacatcatt tatttggctt acacaaaatc acacgagatt ttgctagtca tacgatatct
     1561 gttattcaaa ctggtgtttt tattttggaa gattttagaa atatcctatt gtacaatgat
     1621 gtaatcgaaa ggatatcttt atatgagttt atcggcgata tcttctattc taagctaaca
     1681 agttattgca tcgtagctaa ggatttatct aaaaatacta tgaaattgga cgtcatattt
     1741 tttgaggata aaaataaaag atccgcagtt ttaggtttac gaagagataa aagcggagta
     1801 tttaaaccag ttactctaca ttttacaagc gctaagaaat atgctaaagt tcgtaaaaca
     1861 aatgtgaaag aaatgaaatg gctataaaga ttgtccgcag ccttaagctt gcgttttttg
     1921 ttgtttaacg aaattaaaaa acaagctctc tcatcggaaa tgtttttata ggaatgaagg
     1981 aagatgatgc tatgtttaaa aagagaatcg aaaagggcaa aagtagtgtc tttatctttt
     2041 tggagtagaa gcttatgata tagaaatttg taataaaaat gtaatataaa aca
//
LOCUS       DAVLWA010000145         2074 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLWA010000145 DAVLWA010000000
VERSION     DAVLWA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.145"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..981
                     /locus_tag="VYN80_002013"
     CDS             <1..981
                     /locus_tag="VYN80_002013"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1541859.1"
                     /translation="LTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAP
                     FYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYG
                     SFFGYELPFHLISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGF
                     AWCVILLGLLFIAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLF
                     NLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHA
                     INIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            997..1473
                     /locus_tag="VYN80_002014"
     CDS             997..1473
                     /locus_tag="VYN80_002014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV1541860.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1507..>2074
                     /locus_tag="VYN80_002015"
     CDS             1507..>2074
                     /locus_tag="VYN80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541861.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQ"
BASE COUNT          668 a          280 c          409 g          717 t
ORIGIN      
        1 ttgacggata atcaagatga aatacctatc aaattaacga atcattcttt aattgaacca
       61 tttgaattat tgacagaaat gtatgctctg cccaaatatt atgagaaaga tcctacacct
      121 gtattagcac cattttactt tacatttttt ggaatgatgg ttgctgattt aggctatggt
      181 ttactattgt ttttaggaac aatgttagca ttaaaaattt ttcatctacc ttcagcaact
      241 aagagatttt taaaattctt taatatatta ggggtagccg ttgcaatttg gggtggaatc
      301 tatggctcat tttttggata tgagttgcca tttcatctga tatctacaac ctctgatgtc
      361 atgactatat tagtagtgtc agttgtgttt gggtttatta cagtatttgc aggtttgtta
      421 gcttcaggac tacaaaaagt aagaatgaag aaatatgcag aagcatataa ttcaggattt
      481 gcgtggtgtg ttattctgct tggcttgtta tttattgctg ttggaatgtt gatgcctgat
      541 atgagaccgt tatttgtatt agggaaatgg gtatctattt ttaatgctgt ggggattttg
      601 attgtttcta ttattcaaac caaaagcttg tcaggtattg gagcaggatt gtttaatcta
      661 tataacattt catcttatat aggtgattta gttagtttca ctcgattgat ggcattagga
      721 ttatctggag caagtatagc atcagctttc aatttaattg ttggtttgtt tccgggaata
      781 ttggctaaac tgacaattgg attagtatta ttcattcttt tacatgcgat caatattttt
      841 ctatcgttac tatcaggata tgttcatgga gcacgtctga tatttgttga attttttggt
      901 aagttttatg agggtggagg aaaaccattt caacctttga aggcttctga gaaatatatt
      961 aaggttatta caaagaatta atggaggata tatataatgg aacatttagc aacttatttt
     1021 tcaacctatg gaggagcttt cttcgctgca ttgggaattg tattggcggt tggattaagc
     1081 ggtatggggt ctgcttatgg agttggtaag gctgggcaat ctgccgcagc tttactgaaa
     1141 gaacagcctg aaaagtttgc ctcagctttg atattgcaat tattgcccgg aacacaagga
     1201 ttatatggtt ttgttattgg aattttaatt tggttgcaat taactccaga acttccttta
     1261 gaaaaaggcg ttgcttattt ctttgtagct cttccaattg ctattgtagg atacttttca
     1321 gctaagcatc aaggaaatgt agcagtagcg ggaatgcaaa tcttggctaa aagaccaaaa
     1381 gaattcatga agggagcaat tttagctgcc atggtagaaa cctatgcaat tcttgctttt
     1441 gtcgtatcat tcattttgac ccttcgtgta taagaaataa atttgcaatt caaaggaggt
     1501 gtctaaatga gcaatttaga aaacttacga gagtctgtta ttgaacaagc tcatgaaaaa
     1561 gggcgtatga aattattgga ttccaaaaag aagattgatg atgaatttga aatgcaaaag
     1621 tcgctcatta taaagaaaaa agaagctgaa catgaacgaa agttaaaaga attgcaacag
     1681 aaatatcaaa taatttttca acaattaaaa aataaggaac gccaatcaac gttagtatca
     1741 aaacagaaaa tattaaaaga actttttcaa tctgctttac tagaaatgga atcttggagt
     1801 gcagataaag aaatggagtt catctatcga attctggaac gatattcaca acaagaggtc
     1861 atagtaacct ttggggaacg gactttagct aaattcaatt tggaacaatt agagaaattg
     1921 aaattctctt ttccaaatta tttatttagt gaacaaccta tctcaaatga atcaggctta
     1981 cttatttcaa taggtaaaat tgatgataac tatttgtata aaacattaat tggatcgatt
     2041 tctaaggaag aaagttcaag tatcgcaaat caaa
//
LOCUS       DAVLWA010000146         2043 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLWA010000146 DAVLWA010000000
VERSION     DAVLWA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.146"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(334..759)
                     /locus_tag="VYN80_002016"
     CDS             complement(334..759)
                     /locus_tag="VYN80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541862.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(903..1271)
                     /locus_tag="VYN80_002017"
     CDS             complement(903..1271)
                     /locus_tag="VYN80_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541863.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKVMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKK"
     gene            complement(1293..1499)
                     /locus_tag="VYN80_002018"
     CDS             complement(1293..1499)
                     /locus_tag="VYN80_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541864.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(1486..1647)
                     /locus_tag="VYN80_002019"
     CDS             complement(1486..1647)
                     /locus_tag="VYN80_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541865.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT          647 a          426 c          234 g          736 t
ORIGIN      
        1 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
       61 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
      121 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
      181 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
      241 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
      301 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
      361 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
      421 gttattactt cctaatatcc cattatgctt tttcgctaag gaaatagaag ccacttctcc
      481 cttaccaata atcttgttac tatcatgatt tcttgttaaa tctctatata atgcgtattc
      541 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
      601 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
      661 aacaatttta cccgaataga gcttttctaa aagatgttca gtaccaaccc ataaaaatac
      721 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc cccctcttca
      781 tctagcccat atacaatatc atatctgaaa gcatctagta acagttcctc atacttccct
      841 tgcgaaattc tgttattttc taaaagttgt tcagttgaat taacatatta tcctaatatc
      901 atttactttt tactttctga caaaggacga tataaacttg tatcatagcc taatcttgaa
      961 gctgtctcta taacactaat atccatattt ttaatttctt ctgcatcaag gtatccatca
     1021 ttcctcaatc tatataacat aactttatga ctgataccat aaaactgacc caattttata
     1081 atatcttcta cttcaagatg agttctattg gcattttctc taatttcctc aaccatccta
     1141 tacagtgaag atgggaaaat taaaaaataa gaagcaaact gatccgcttt tctttcagtt
     1201 tcatctcctt caccaatcaa gataagactg actgaactct tcttcacctc atcataataa
     1261 agatgataca gttcatgtgc taaagaaaat ctttaccttc ctaatgacat gtctgaattg
     1321 actgcaatga gactgaactg agttccttta taacagactc cgctaatatt ctttccgagt
     1381 ccataaaata ccagcgtcaa attttctatc ttttgtacca atttaaaaat atctatcggc
     1441 gattcaccat cagctcccaa cttttttcta agatttgaag ctttattact taaatccatt
     1501 cgtgatattc ctttcacctt tttccatttt ggcaatcatg ctttgattca aagacaaata
     1561 ctcagcaatc ttatcttgag taagatttga agatattcta agttctttta ttctttttcc
     1621 aacatcacat acatttatca catacattta tcatattcat acacctctca taaaaagaat
     1681 agcacactct tgtcataatt ttttaaataa aaaaattatg acaaaacaag gaagcaattt
     1741 attgatgctg cttaaaaatc taaaattgat gagttcctat caaaaatcgt atcttcaacc
     1801 tcaaaacagt acttaaagct atccgactcg gtttacattg tcaaatttag attttatttg
     1861 agcataactt tctagtttgc tttttgattt ttgtttaata tagtagcaaa attgaaggaa
     1921 atatctccac aagaaagcgc atactattaa gctttttcaa gacctaataa tatgcgctgt
     1981 tctgatttga aagacattcc attattattt tactgtaatc aagccatctg gctctactgt
     2041 gaa
//
LOCUS       DAVLWA010000147         1949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLWA010000147 DAVLWA010000000
VERSION     DAVLWA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.147"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..331
                     /locus_tag="VYN80_002020"
     CDS             <1..331
                     /locus_tag="VYN80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541866.1"
                     /translation="GAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAM"
     gene            443..1048
                     /locus_tag="VYN80_002021"
     CDS             443..1048
                     /locus_tag="VYN80_002021"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541867.1"
                     /translation="MDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSP
                     KYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTL
                     GRLAIYHENKNPYVWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGST
                     KQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1105..1902
                     /gene="psrA"
                     /locus_tag="VYN80_002022"
     CDS             1105..1902
                     /gene="psrA"
                     /locus_tag="VYN80_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1541868.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          696 a          284 c          342 g          627 t
ORIGIN      
        1 tggtgcaata cacgatggat ggttggctat ttcgaactat gaaaactcat taaataaaga
       61 ttacctattc tatattcttt catcaaatgt agtttattct caatttctat ctctaattag
      121 tggagctgtt gtgaaaaact tgaatagtga taaagttgct tctattctta tccctctccc
      181 cccactatcc gaacaacaac gcataataga agcaatcgaa tcagctttag aaaaagtaga
      241 tgaatatgct gaaagttata atagactaga acagctagat aaagaatttc cagataaact
      301 aaaaaaatct attcttcaat atgctatgta aggaaaatta gttgaacaag acccaaatga
      361 tgaatcagtc gaagttttac ttgaaaaaat acgagcagaa aaacaaaaac tctttgaaga
      421 aggcaagatt aaaaagaaag atttggacat ttctattgtt tcccaaggag atgataactc
      481 ttattatgag gaagtacctt gtgaaatacc agaaagttgg gaatgggtga ggttaaacga
      541 tataacctcc tacattcaaa gagggaagtc tccaaaatat tcaaatattc ctatataccc
      601 tgtgattgct cagaaatgta atcaatggtc tggcttttct attgacttag caagatttat
      661 tgatcccgaa acagttcata gttaccagaa agaaagatta ctaagagatg gtgatttaat
      721 gtggaattca acaggtctag gtacgttagg acgattagct atttaccatg aaaataaaaa
      781 tccttatgtc tgggctgttg cagatagcca tgttacagtt ataagagtct tatcgggagt
      841 tattaattgc catttcatct ataattttct ttcctctcct attgttcagt cagttattga
      901 agaaaaagcg tcgggtagta caaaacaaaa agaattatta acgaaaacta taaaagaata
      961 tttaatcccc ctcccacccc ttcctgaaca atccagaatc gttgataaaa tcgaacaatt
     1021 tttcgcccat attgacgcac taatttagta taatagtttt acgtgcggtg gaatttctat
     1081 caaacataag ataggagttt tacgttgaaa gattttgaat tacatttgaa gaaagctggt
     1141 ttagctgaaa atactgtgcg ttcgtacctt tatggtgttc gatttttctt ggagaattac
     1201 gaactcaaaa tggaagattt atttgaatac aaaggatatt tattagacaa ttttaaacct
     1261 aaaactgtca atttgcgact acaaggagta aataaatatc tggcttttat tggtcatgat
     1321 gatttgaaat taaaatttgt caaagtacag caaaaaccgt ttttagaaga tgtcattagc
     1381 catgctgatt acctttttct taaacgtagt ttgaaaaaag atggtatttt aaaatggcat
     1441 tttgtcgttt ggtttttagg tgcaactggg gctcgtgtaa gtgaacttat taaactaaaa
     1501 gtagagcatg tagaaatcgg ttattttgac atttactcaa aaggtggtaa aattcgtaga
     1561 ctgtacattc ctaaaaaatt aagaaatagt tgtcttagtt ggttagaatc agaaaatcgc
     1621 cgaagtggtt atttattttt gaataaattt aacgaaccaa ttacagcaag aggagttgct
     1681 caacagttaa aaaattatgc tgataaatac aaaatgaatc ctaaagtaat ttaccctcat
     1741 tcttttaggc atttatttgc taagaatttt ttagcgaagt ataatgatat tgccttgctt
     1801 gcagatttga tgggacacga aagtatagaa actactcgaa tttatctaag gaaaacagct
     1861 actgaacaac aaaatattgt agataaaatt gttaattggt aaaaaataac aggtggtcaa
     1921 actgactacc tgctattttt gtgattatg
//
LOCUS       DAVLWA010000148         1948 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLWA010000148 DAVLWA010000000
VERSION     DAVLWA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.148"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..552
                     /locus_tag="VYN80_002023"
     CDS             <1..552
                     /locus_tag="VYN80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541869.1"
                     /translation="SFATLLIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLP
                     LFGILIIYFIWLLIEGLKYIRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYID
                     LYESCRQNNLSVLLNFLQNSDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARE
                     NVLNKEDAEKLLNEIDKKLEISR"
     gene            608..1303
                     /locus_tag="VYN80_002024"
     CDS             608..1303
                     /locus_tag="VYN80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541870.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1315..1731
                     /locus_tag="VYN80_002025"
     CDS             1315..1731
                     /locus_tag="VYN80_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1541871.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT          610 a          293 c          434 g          611 t
ORIGIN      
        1 tcttttgcaa ctctattaat agtgacaaat ttgttatcca ttattcatta ttttggagcc
       61 aaaaatttaa tagtttcttt taatgaaact tatcctcgta ttcaatccag tgttggtatc
      121 atgtggctac cactgtttgg tattctgata atctatttta tttggctctt aatagaggga
      181 ttaaaatata tcagaaaaat aaattatatt attcaaaata gggaaataat ctatttttat
      241 tcgcaggtaa aagttgtaga taaacttact gattctgaat cgaaaaaata cggtgaatat
      301 attgatttgt acgaatcgtg tcgtcagaac aatttaagtg ttctacttaa ctttcttcaa
      361 aattcggatg ataaacgagg agttattgct agtaactcag tgagaattat gtttgaatct
      421 ttggataaag cagaccaaat tgcggccctg aatatttgta gaaactatgt agcacgagaa
      481 aatgttttaa ataaagaaga tgcagaaaaa ttgttaaatg aaattgataa aaaacttgaa
      541 attagcagat aggacaattc aaatagagtt gagtaaaaag tttgaaaagt gaaacaagga
      601 gaaagctatg ccagtaaatg aatatggtca gatgattggt gagtcaatgg aaggttatac
      661 acccggtgca ctgccttcta ttgatttctt agaagggcgt tatgctagga tagaggctct
      721 ctcggtagaa aaacatgcgg aggatttgct agctgtttat ggcccggata ctcctcggga
      781 gatgtggacc tatctttttc aggagccagt agcagatatg ggggagctgg ttagcctttt
      841 aaatcagatg ttggctcgta aggaccgttt ttactatgca atcatagaca aggcgactgg
      901 taaggctttg ggaacttttt ctctcatgcg cattgaccag aataaccgag taatagaagt
      961 cggagcagtc actttttctc cagagctaag gggtacacgg ataggaactg aggctcagta
     1021 tctcctagct cgctatattt ttgaggagct aaactatcgt cgctacgagt ggaaatgcga
     1081 tgccttaaat ctgccatcca gacgagttgc ggagcgtttg ggatttgtct atgaaggaac
     1141 cttccgccag gcggtggttt ataaggggcg tacgagagat acggattggt tgtctatgat
     1201 tgatatggac tggcctcaag tcaaagatcg tttggaaaca tggctgcgtc ctgaaaattt
     1261 tgataaaaat ggacagcagt acaagagctt gagagaactt taagaggtgt tgagatgatt
     1321 actattaaaa agcaagaaat tgtcaagcta gaggatgttt tgcatctcta tcaggctgtc
     1381 ggttggacaa attataccca tcaaccagag atgctggagc atgccttatc gcattcatta
     1441 gtaatttatc tggcacttga tggtgatgct gtggtgggct tgattcgttt ggttggagat
     1501 ggattctcat cagtttttgt acaggatttg attgttttac caatctatca gcgtcaaggg
     1561 attggtagtg ccctaatgaa agaggcctta aaagattaca aagatgctta tcaagtccag
     1621 ctggtgacag aagagacaga aaaaaacgtg ggattttatc gttctatggg ctttgaaatc
     1681 ttatccacct ataattgtat agggatgact tgggcgaatc gagaaaaata acaaaacttg
     1741 ttttttctta agcaaaattt aaggatggtc tagtattata tagtcattaa ataaagacct
     1801 cctaacttta tttaataaaa tcctaaactt ttttcatcac aatctcctaa tgaagccacc
     1861 caatcaggtg gcttttttgc gtggtgaggt gctggtgata gaaatttttt gcaaaatagt
     1921 aaaattttag aaaagttaag ctagtttt
//
LOCUS       DAVLWA010000149         1865 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLWA010000149 DAVLWA010000000
VERSION     DAVLWA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.149"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(125..1756)
                     /locus_tag="VYN80_002026"
     CDS             complement(125..1756)
                     /locus_tag="VYN80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1541872.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          573 a          369 c          388 g          535 t
ORIGIN      
        1 aggtgtacta ttctagtttc aatatactat atatctacag aatactcttt cagtttcatt
       61 cagcctagtc ttgcttagga aaaagtaaaa agccatccaa ttatgatgga tagcttcttg
      121 gttcttagat ttgttctgca aaaacctttt cagcaaggtt catagcgtgg tctgatacac
      181 gagtgtagtg tgagatgatg tcgataaagt tgaccccagc ttgtgttgaa cattcgcctt
      241 tgttgagacg tttgatgtgg gtttttctga gaacacgttc tatcttattg attgcttcat
      301 gacgttctac aagactgcgt gctttttcaa tatcattgtt ttccacacta tccagagcat
      361 ctttgataaa gtcactagtt tggcggtaaa cttcctctaa ttctttcaag gcggcatcag
      421 aaaattcaac atttttccgt tgaagatagt cagtcagatt gagtagagcc tccgtgtggt
      481 ctccaatccg ttccaaatca cgggaggaat caaggatatt ggtaagcact tcactttctt
      541 tttggctgag agcttcgctt gaaagggcaa tgagataacg tgttaattgc tcatcgatgg
      601 tgttaattgc ttcttcggtt ttatgccctt tttcagcaac tttttcatcc aagtcaatga
      661 tgtacttata ggaaaggtca aaggctttag cagcgtagtt tcctaagtgc aagagctctt
      721 tcttagcatt tcctagagcg atagatgggg cctgtttgat gaaatgttca tcaagatata
      781 agggttcgta tttgacaacc tcgtcctctc caggaataat cttggttaca aagtaagcca
      841 gagctccgat aaatggaaat tggacaatgg tgttggtaat attaaaggtt ccgtgagcaa
      901 aggcgatggt catttccggt gctagattta gcgtagcttc aaaccaatga atcaggacag
      961 taaaaggaac tagaaaaata acgcagacga ctgttccgat aacgttgaag gcaacatgag
     1021 ctcctgctac ccgtttagct gcaatattag cccctaaaga ggcaatgatg gctgtaatgg
     1081 ttgtcccgat attgtcacca aatagaactg gcaaagctcc ctgtaggtca attagattgc
     1141 cggcgtagag gttttgtaaa atcccaatgg tagccgaaga agcttgaatt agcaaggtca
     1201 agccagtacc gacaaagaca cccaaaacag gattcttact tagctcaatc atatagtcct
     1261 taaagacctg taaatccttg agtggagcca ttgcgccgct catgagattg agggcaaaaa
     1321 agataccacc gacaccaaag aggatgcgtc cgatattatt gactgtccga tttttcgtaa
     1381 aaaagagaca gacggcaccg ataaagagca taggtagggc atagttacct aatttaaaac
     1441 cgatgagaaa ggatgtgaca gttgtcccaa tattagcacc catgacaatc ccgatagcct
     1501 gacgtaaggt taagagaccg gcactgacca ggccgactgt aataactgtt acaccagaac
     1561 tagactgaat tagagcagtc atcccaatac caaccagaac tccaaagaaa ggattactag
     1621 tatatttgtc aatgtaaaaa cgaaggcgat ctccagcagc ttgttgcaaa ccgtctccca
     1681 tggtcttgat actatataag aatagcccca gaccacctaa aaagtgaaat aaaatttcct
     1741 gccaattaat ggacatttct ttttcctccg aaaaataatc gcggaatatc tcctattcta
     1801 ttttaaagga taaaagtaaa tctaacaagt gttaacgtca agtttgtaaa agaaaattat
     1861 aattg
//
LOCUS       DAVLWA010000150         1845 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLWA010000150 DAVLWA010000000
VERSION     DAVLWA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.150"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            105..1451
                     /gene="gdhA"
                     /locus_tag="VYN80_002027"
     CDS             105..1451
                     /gene="gdhA"
                     /locus_tag="VYN80_002027"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1541873.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSIWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          571 a          340 c          377 g          557 t
ORIGIN      
        1 gaattgaaag aatttttaga aaatttctgt tttttcttta cagtgaaaaa aaattctgct
       61 ataatgtctt atgtaataaa atatgacaat aaaaggagaa cgatatgaca tctgctaaag
      121 aatatatcca aagcgtgttt gaaactgtga aagctcgtaa cgggcacgag gctgaattcc
      181 tccaagctgt tgaagaattt ttcaacactt tagaacctgt atttgaaaaa caccctgagt
      241 atatcgaaga aaatatcttg gcacgtatta ctgagcctga gcgcgtggtt tctttccgtg
      301 ttccttgggt tgaccgtgat ggaaaaattc aagtaaaccg tggttaccgt gttcaattca
      361 actcagctgt tggaccatac aaaggtggac ttcgtttcca cccaactgta aaccaaggga
      421 tcttgaaatt cctcggattt gaacaaatct ttaaaaatgt cttgactgga cttcctatcg
      481 gtggaggtaa aggtggatca gacttcgatc ctaaaggtaa aacagatgct gaagtgatgc
      541 gcttctgcca aagcttcatg actgaattgc aaaaacacat cggaccatca cttgacgtac
      601 ctgctggtga tatcggtgtt ggtggacgtg aaattggtta cctttacggt caatacaaac
      661 gtcttaacca atttgatgct ggtgtcttga ctggtaaacc tcttggattt ggtggtagct
      721 tgattcgtcc agaagcaact ggttacggtt tggtttacta tactgaagaa atgctcaaag
      781 ctaacggtaa cagctttgct ggtaagaaag tcgttatttc aggttctggt aacgttgctc
      841 aatatgctct tcaaaaagca actgaactcg gtgcaactgt tatttctgtt tctgactcaa
      901 atggttatgt catcgatgaa aatggtatcg acttcgatct tttggttgat gttaaagaaa
      961 aacgtcgtgc tcgtttgact gagtatgcag ctgaaaaagc aactgctact tatcatggag
     1021 gttctatatg gacttacgct ggtaactatg atatcgctct tccatgtgcg actcaaaacg
     1081 aaatcaacgg tgaagcagct aaacgtttgg ttgatcaagg cgttatctgt gtatctgaag
     1141 gtgccaacat gccaagcgac cttgatgcca tcaaagttta caaagaaaat ggtatcttct
     1201 acggacctgc caaagctgcc aacgctggtg gtgtagccgt ttcagccctt gaaatgagcc
     1261 aaaatagtct tcgcctctca tggactcgtg aagaagttga tggccgtctc aaagacatca
     1321 tgacaaacat cttcaacaca gctaaaacga cttcagaaac atatggtctt gataaagact
     1381 accttgcagg agcaaacatt gctgcctttg aaaatgtagc aaacgctatg attgcccaag
     1441 gtattgttta agatttattt gcttaatcct caatcactac gattggggat ttttagttga
     1501 gttagacagt tgggaaattc taattaaatg aaataaaaac tgaacaaatt gattgcggaa
     1561 ttcaaaccaa tttctaacat atttctagaa attaatttga cttttctaat cgatttgttc
     1621 atatcttatt tcaatttact atacttactt aacccttttt atactcaatg aaaatcaaag
     1681 agcaaactag gaagcgagcc gcaggctgta cttgagtacg gcaaggcaaa gctgacgtgg
     1741 tttgaagaga ttttcaaaga gtattaaaca aaaagaaaaa ccgctactcc taagaatagc
     1801 gatttaatca tgtttttaag ctactactgt agttgctcta ttatt
//
LOCUS       DAVLWA010000151         1813 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLWA010000151 DAVLWA010000000
VERSION     DAVLWA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.151"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1013
                     /locus_tag="VYN80_002028"
     CDS             <1..1013
                     /locus_tag="VYN80_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1541874.1"
                     /translation="YWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKLVAV
                     SVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMGIDV
                     TVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLVLFAI
                     ASLVLVPLIRKDGGPAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTMQGG
                     MFKMDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPEQKR
                     RLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFIRDG
                     AK"
     gene            1017..1466
                     /locus_tag="VYN80_002029"
     CDS             1017..1466
                     /locus_tag="VYN80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEV1541875.1"
                     /translation="MKVCLVGSSGGHLTHLYLLKPFWNDKERFWVTFDKADARSVLSE
                     EVVYPCYYPTNRNIKNLIKNTFLAYKILKKERPDLIISSGAAIAVPFFYIGKLFGSKT
                     VYIEVFDRIDASTLTGKLVYPVTDRFIVQWEEMKKVYPKAINLGGIF"
     gene            1466..>1813
                     /locus_tag="VYN80_002030"
     CDS             1466..>1813
                     /locus_tag="VYN80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEV1541876.1"
                     /translation="MIFVTVGTHEQQFDRLIKEVDYLKKENLIQDEVFIQIGYSSYIP
                     KYCEWEKIISYEKMNQLIKESDIIITHGGPATFMGVIAKGKVPIVVPRQKKFGEHVND
                     HQLQFVKLTKEIYN"
BASE COUNT          621 a          232 c          376 g          584 t
ORIGIN      
        1 aatattggaa gcatgtgttt tttaatctaa aaaatagcaa gaaaatttta ctgttaacag
       61 taacgaaaaa tatggaaaaa gttcttgata aattgctaga atctgatgaa ctttcatgga
      121 aattggtagc agtaagtgtt ttggataaat ctgattttca acatgataaa atacctgtaa
      181 ttgaaaagga aaaaattatt gaatttgcaa cgcatgaagt tgtggatgag gtgtttgtca
      241 atcttccagg agagagctac gatattggag aaattatctc taggtttgag acaatgggga
      301 tagatgtaac tgtaaatctt aaagcatttg ataagaattt gggtcgcaat aaacaaattc
      361 atgagatggt aggattgaat gtagtcactt tctctacaaa tttttataaa actagtcatg
      421 tgatttcaaa gagaattctc gatatttgtg gtgccactat tggccttgtt ctttttgcta
      481 tagctagtct agttttagtt ccattgattc gtaaagatgg cggaccagct atttttgctc
      541 aaactcgtat agggaaaaat ggtcgacatt ttacctttta taaattccgt tcgatgcgga
      601 tcgatgctga agctatcaaa gaacaattga tggatcaaaa tacgatgcaa ggtggtatgt
      661 ttaagatgga caatgatcct cgtgttacaa aaattggtcg ctttattcgt aaaaccagtt
      721 tggatgaatt gccgcagttt tggaatgtct ttataggaga tatgagtttg gtgggaacac
      781 gtccacctac agtagacgag tatgatcagt atactccaga acagaaacgt cgactcagct
      841 ttaaacctgg tattacaggt ttatggcagg ttagcggccg tagtaaaata accgattttg
      901 acgatgttgt aaaattagat gtggcttata ttgataattg gacaatctgg aaagatattg
      961 aaattttgct taaaactgtt aaagttgtat ttataagaga tggagcgaag taaggaatga
     1021 aagtatgtct agttggatct agtggaggcc atttaactca tttgtattta ttaaaacctt
     1081 tctggaatga taaagaaaga ttttgggtta cttttgataa ggcggacgct cggagtgtac
     1141 tctctgagga agttgtatat ccctgttatt atccaaccaa tcgtaatata aaaaatttaa
     1201 taaaaaatac ttttctcgcc tataaaattt taaaaaaaga gagaccagat ttgattattt
     1261 cttctggtgc cgctatcgct gttcctttct tttatattgg gaagttattt ggatcaaaga
     1321 cagtgtatat agaagtgttt gatagaattg atgcttcaac actgacagga aaacttgttt
     1381 atccagtaac ggataggttt attgtacaat gggaagaaat gaaaaaagta tacccaaaag
     1441 caattaacct aggagggata ttctaatgat ttttgtgact gtaggtacac acgaacaaca
     1501 atttgatcga ttgattaaag aagttgatta tctaaaaaaa gaaaatctta ttcaagatga
     1561 agtatttatt caaataggat attccagtta tattccgaaa tattgtgagt gggaaaagat
     1621 aatttcttat gaaaaaatga atcaattgat taaggaatca gatattatca ttactcatgg
     1681 tggaccagcg acgtttatgg gggttattgc taaaggaaaa gttccgatag ttgttccgag
     1741 gcaaaaaaaa tttggagagc atgtgaatga ccatcagctt caattcgtaa aactgacgaa
     1801 agaaatatac aat
//
LOCUS       DAVLWA010000152         1756 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLWA010000152 DAVLWA010000000
VERSION     DAVLWA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.152"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(1..632)
                     /locus_tag="VYN80_002031"
                     /pseudo
     CDS             complement(1..632)
                     /locus_tag="VYN80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            995..1594
                     /locus_tag="VYN80_002032"
     CDS             995..1594
                     /locus_tag="VYN80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541877.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEGTGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT          583 a          283 c          339 g          551 t
ORIGIN      
        1 gcgacatcgg acaactctat cgtagcgaag aaagtgattt cgaatgatag cttgtgttct
       61 accctcaaga acagtgataa tatcgagctt ttcaaaatct tgcgcaatga agctcatctc
      121 catctcccga ttgaaacagt cactccccgg actgtttcaa cctcccaaga cataatctca
      181 ggcagatttc taaaattaca ctcaaagtgg aagtcattga gcttgcgaat gacagttgaa
      241 gttgaaatgg ccagctgatg ggcaatatca gtcatagaaa ttttttcaat taacttttga
      301 gcaatttttt ggttgatgat acgagggatt tggtgatttt tctttaccag gggagtctca
      361 gcaaccatca tttttgaaca gtgatagcac ttgaaacggc gttttctaag gagaattcta
      421 gaaggcatac cagttgtttc gaggtaaggg atcttagacg gtttttgaaa gtcatatttc
      481 ttcattagac ttccacaatc agggcaagat ggagcctcat aatccagctt agcgataatt
      541 tccttgtgtg tatccttatt gatgatgtct aaaatctgga tattagggtc tttaatgtct
      601 agtaattttg tgataaaatg taattgttcc atatgaatct ttctaatgag ttgtttggtc
      661 gcttttcatt atagatctta tgggactttt tttctacaat aaaataggct ccataatatc
      721 tatagtggat ttacccacta caaatattat agagccgtta atatttggag aagaggatgg
      781 ccatcttctc catttttatg tgcagagtta tagtgacttg atgctgggaa agtacactgt
      841 gactgctaat actcaatgaa aatcaaagtg caaactagga agctagccgc aggctgtact
      901 tgagtacgtc aaggtgaagc tgacgtggtt tgaatttgat tttcgaagag tataaaacat
      961 tcctagaagt tactttgatt tccctaatct atttatgcaa attttatgtt attttactat
     1021 aacagttgtt gctaagccaa ataatagtgg ggaagttcat ttagacgtaa gcattgaaga
     1081 taatcaggga ggtagtgggc ataatttcag ttctgtttca agtagctcac aaacagctaa
     1141 atatgaagga actggttata ataacaattc atcattatat ataacgattg ataaaacgtc
     1201 tgatgcaaca gctcttttga aattaaagtt gaataatgtt gataatcaac ctgctactga
     1261 agttcctagt tcaggaatta ctgtaaaatt aaatgctaaa gataatgctg gaaactggac
     1321 aagtgcttcg aataaaaaag aagtaacagt aaaaattgtt tctgctaaac cgacatatcc
     1381 agacaaaatc ttagtgaaaa atcctgataa tataaaagat acagaaaaaa atgccattat
     1441 tgaaaaattg aaagaggcaa ataaaaatca tccagcagga gctccaacct ttgctaaagg
     1501 tgaaggagag catgcaaatg atattgtagc aacttattca gatggtacaa cttattatgt
     1561 accgttaaat gatgtgacaa aatatgcgag gtagtggctg taccactcac ttattcacct
     1621 ccccgtgatt tgtagtagtg ataggctttc tcactattat tataaaacaa aataaagatc
     1681 acaacacttt ttcattctgt gttgtgcctt gagtgaaacg aaaggaatga attataaata
     1741 tgaaaagtat agtcac
//
LOCUS       DAVLWA010000153         1742 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLWA010000153 DAVLWA010000000
VERSION     DAVLWA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.153"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            319..795
                     /locus_tag="VYN80_002033"
     CDS             319..795
                     /locus_tag="VYN80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1541878.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            795..1556
                     /locus_tag="VYN80_002034"
     CDS             795..1556
                     /locus_tag="VYN80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1541879.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          638 a          235 c          341 g          528 t
ORIGIN      
        1 gcctaataat cacatgtgga gtgttgctac aacgaaaaag gtgataatcc ttaatttcaa
       61 gctattttat aagcattttg tctttgtaga taaaggcaat tttgacaata aaaatcctaa
      121 aaggtgaatc gttatagatg tatttgtaga tattgtttgc gcatcgaaaa aattaataca
      181 agaataaata tttatagctc tttaggtgac ttttatagaa gtaaagttta ggatagaaaa
      241 acaagaaata acgcaccatt tttggtgcgt tatgcttttt tatgctataa tggatttata
      301 aaaataaagg agtttgctat gattggaaag aacataaaat ccttacgtaa aacacatgac
      361 ttaacacaag acgacttttc ccgaattgta ggaatttctc gaaatagctt gagccggtat
      421 gaaaatggaa ctagttcagt ctctacggaa ctaatagaca tcatttgtca gaagtttaat
      481 gtatcttatg tcgatattgt aggagaagat aaaatgctca atcctgttga agattatgaa
      541 ttgactttga aaattgaaat tgtgaaagaa agaggtgcta atctattatc tcgactctat
      601 cgttatcaag atagtcaggg aattagcatt gatgatgaat ctaatccttg gattttaatg
      661 agtgatgatc tatctgactt gattcatacg aatatctatt tagtagaaac ttttgatgaa
      721 atagagagat atagtggcta tttggatgga attgaacgta tgttagagat atctgaaaag
      781 cggatggtag cttaatggaa atccaagatt atactgatag tgaattcaaa catgctttag
      841 caaggaatct tcgttcactg acaagaggaa aaaagtccag taagcaacct atagcgattt
      901 tgcttggagg gcaaagtggt gccggtaaga ctacaattca tcgtattaaa cagaaagaat
      961 ttcaaggaaa tattgttatc atagatggtg atagttttcg ttctcagcat ccacactatt
     1021 tagaactgca gcaagaatat ggcaaagaca gtgtagaata taccaaagat tttgcaggaa
     1081 aaatggtaga gtctttagta acaaaattga gtagtttgag atacaatctt ttgatcgagg
     1141 gaactttacg aacagttgat gttccaaaga aaacagcaca actcttgaaa aataagggat
     1201 atgaagtaca attggcctta attgcgacaa agcctgaatt gtcgtatcta agtactctta
     1261 tccgttatga agaactgtac attatcaatc caaatcaagc acgcgcaact ccaaaagaac
     1321 atcatgattt cattgtaaat catctagttg ataatacacg acaattggaa gaactggcta
     1381 tctttgaaag cattcaaatt taccaacgag atagaagttg tgtatatgat tcaaaagaaa
     1441 atacaacttc agcagcagat gttcttcaag agttactctt tggggagtgg agtcaggtag
     1501 agaaggagat gttgcaggtg ggggaaaaga gacttaatga attacttgaa aaataaacaa
     1561 ttgatatttt taggagaata gaaatgagag ggtttaataa caagataaag tctgtttatc
     1621 aagaactaac aaattccaaa gagaaattcg gtagctttca caagacttta attcatttgc
     1681 atacacctgt ttcttatgat tacaagctat tttctaattg gactgcaacg aaatatagaa
     1741 aa
//
LOCUS       DAVLWA010000154         1686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLWA010000154 DAVLWA010000000
VERSION     DAVLWA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.154"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..76
                     /locus_tag="VYN80_002035"
     CDS             <1..76
                     /locus_tag="VYN80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1541880.1"
                     /translation="VYHYRVAYRLYLEKLVMNRGFISC"
     gene            307..1593
                     /locus_tag="VYN80_002036"
     CDS             307..1593
                     /locus_tag="VYN80_002036"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1541881.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT          473 a          247 c          395 g          571 t
ORIGIN      
        1 cgtttaccat tatcgtgttg cttatcgttt gtatcttgaa aaattggtaa tgaatcgggg
       61 ttttatttct tgttgaggta attttagtaa atttccgaac taatttactc ttttatgtaa
      121 agataatagt aaatagctag taatttttct aaatcatttt ttaatagttg gaaatagcaa
      181 atctttctat tgtttcttct tgataaaaag gcgatttttt attataataa attgtaagat
      241 ataattgcag gtgagagtcc tgccatgtat gtgagaaagg aagagcctga gggctcagac
      301 aaaattatga cttcagttgt tgttgtaggt acccaatggg gtgatgaagg taaagggaag
      361 attacagact tcctttcagc gaatgcagaa gtgattgcac gttaccaagg tggtgataat
      421 gctggtcaca cgattgtgat tgacggtaag aaatttaagt tgcacttgat tccatctggg
      481 attttcttcc ctgaaaaaat atctgtcatt gggaatggta tggttgtaaa tcctaaatct
      541 cttgtaaaag agttgagcta tcttcatgag gaaggtgtaa caactgataa cttgcgtatt
      601 tctgatcgtg cgcatgttat tttgccttat catatcgagt tggatcgctt gcaagaagaa
      661 gctaagggcg acaataagat tggtacgaca attaagggaa ttggtccagc ttatatggac
      721 aaggctgctc gtgttggaat tcgtattgca gatcttttag ataaagatat tttccgtgag
      781 cgtttagaac gtaaccttgc tgaaaagaat cgtctttttg aaaaattgta tgacagtaaa
      841 gcgattgttt tcgatgatat ttttgaagaa tattacgaat atggtcaaca aatcaagaaa
      901 tacgtgatag atacatctgt tatcttgaat gatgcgcttg ataatggcaa acgtgtgctt
      961 tttgaaggtg cacaaggtgt tatgctagat atcgaccaag gtacttatcc atttgttacg
     1021 tcatcaaacc ctgtagctgg tggtgtgaca attggttctg gtgtcggtcc aagcaagatt
     1081 gacaaggttg taggtgtatg taaagcttat acgagtcgtg taggagatgg tcctttccca
     1141 actgagttgt ttgatgaagt gggagaacgt atccgtgaag tgggtcatga gtatggtaca
     1201 acaactggtc gtccacgtcg tgtaggttgg tttgactcag ttgtgatgcg tcatagccgt
     1261 cgtgtttctg gtattactaa cctttctttg aactctattg atgttttgag cggtttggat
     1321 actgtgaaaa tctgtgtggc ctatgatctt gacggtcaac gtattgacta ctatccagct
     1381 agtcttgagc aattgaaacg ttgcaagcct atctatgaag agttgccagg ttggtcagaa
     1441 gatattaccg gagttcgcaa tttggaagat cttcctgaga atgcgcgtaa ctatgttcgt
     1501 cgtgtgagtg aattggttgg cgttcgtatt tctactttct cagtaggtcc tggtcgtgaa
     1561 caaacaaata ttttagaaag tgtttggtcc taagagattt ttaagatttg tttaagatag
     1621 gtcgggtata ctatagacag ttacaagaag acctcctaac ttgttgtaac aaatatccta
     1681 aacttt
//
LOCUS       DAVLWA010000155         1612 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLWA010000155 DAVLWA010000000
VERSION     DAVLWA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.155"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            343..564
                     /locus_tag="VYN80_002037"
     CDS             343..564
                     /locus_tag="VYN80_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541882.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            648..1451
                     /locus_tag="VYN80_002038"
     CDS             648..1451
                     /locus_tag="VYN80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1541883.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
BASE COUNT          521 a          332 c          303 g          456 t
ORIGIN      
        1 caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag ttcttagcgt
       61 ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa tactttgagt
      121 acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa gggtatgctc
      181 ttgtcgaaac tttttttgac tcacttgacc atctaaatac taccatgaaa aagaatatcc
      241 tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg gatcaagccc
      301 ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt ctatggactt
      361 ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt cgtaaacttt
      421 attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt tatactaagt
      481 tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt tcaagatgat
      541 gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt gaaatccact
      601 tccttttagg gtacaatggt tataaatgaa tttttgagag gatcagaatg aaaaaactag
      661 caacccttct tttactgtct actgtagccc tagctgggtg tagcagcgtc caacgcagtc
      721 tgcgtggtga tgattatgtt gattccagtc ttgctgctga agaaagttcc aaagtagctg
      781 cccaatctgc caaggagtta aacgatgctt taacaaacga aaacgccaat ttcccacaac
      841 tatctaagga agttgctgaa gatgaagccg aagtgattct ccacacaagc caaggtgata
      901 ttcgcattaa actcttccct aaactcgctc ctctagcggt tgaaaatttc ctcactcacg
      961 ccaaagaagg ctactataac ggtattacct tccaccgtgt catcgatggc tttatggtcc
     1021 aaactggaga tccaaaaggg gacggtacag gtggtcagtc catatggcat gacaaggata
     1081 agactaaaga caaaggaact ggtttcaaga acgagattac tccttatttg tataacatcc
     1141 gtggtgctct tgctatggct aatactggtc aaccaaacac caatggcagc cagttcttca
     1201 tcaaccaaaa ctctacagat acctcttcta aactccctac aagcaagtat ccacagaaaa
     1261 ttatcgaagc ctacaaagaa ggtggaaacc ctagtctaga tggcaaacac ccagtctttg
     1321 gtcaagtgat tgacggtatg gatgttgtgg ataagattgc taaagccgaa aaagatgaaa
     1381 aagacaagcc aactactgct atcacaatcg acagcatcga agtggtgaaa gactacgatt
     1441 ttaaatctta aaaaccaaaa aaatacagta tccacattcg gtactgtatt tctggctcta
     1501 taatatctat agtgggtaaa tccactatag atattatgga gcctatttta ttgtagaaaa
     1561 aaagtcccat atgacctata atgaaaagcg acaaaacaac tcattagaaa ga
//
LOCUS       DAVLWA010000156         1584 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLWA010000156 DAVLWA010000000
VERSION     DAVLWA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.156"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(158..245)
                     /locus_tag="VYN80_002039"
     tRNA            complement(158..245)
                     /locus_tag="VYN80_002039"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Ser,seq:gct)
     gene            complement(252..325)
                     /locus_tag="VYN80_002040"
     tRNA            complement(252..325)
                     /locus_tag="VYN80_002040"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ile,seq:gat)
     gene            complement(361..431)
                     /locus_tag="VYN80_002041"
     tRNA            complement(361..431)
                     /locus_tag="VYN80_002041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Gly,seq:tcc)
     gene            complement(451..523)
                     /locus_tag="VYN80_002042"
     tRNA            complement(451..523)
                     /locus_tag="VYN80_002042"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Phe,seq:gaa)
     gene            complement(527..600)
                     /locus_tag="VYN80_002043"
     tRNA            complement(527..600)
                     /locus_tag="VYN80_002043"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(564..566),aa:Met,seq:cat)
     gene            complement(611..700)
                     /locus_tag="VYN80_002044"
     tRNA            complement(611..700)
                     /locus_tag="VYN80_002044"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(662..664),aa:Ser,seq:tga)
     gene            complement(711..784)
                     /locus_tag="VYN80_002045"
     tRNA            complement(711..784)
                     /locus_tag="VYN80_002045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Ile,seq:cat)
     gene            complement(798..871)
                     /locus_tag="VYN80_002046"
     tRNA            complement(798..871)
                     /locus_tag="VYN80_002046"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(835..837),aa:Met,seq:cat)
     gene            complement(877..950)
                     /locus_tag="VYN80_002047"
     tRNA            complement(877..950)
                     /locus_tag="VYN80_002047"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Pro,seq:tgg)
     gene            complement(966..1039)
                     /locus_tag="VYN80_002048"
     tRNA            complement(966..1039)
                     /locus_tag="VYN80_002048"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Arg,seq:acg)
     gene            complement(1049..1134)
                     /locus_tag="VYN80_002049"
     tRNA            complement(1049..1134)
                     /locus_tag="VYN80_002049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1098..1100),aa:Leu,seq:taa)
     gene            complement(1142..1213)
                     /locus_tag="VYN80_002050"
     tRNA            complement(1142..1213)
                     /locus_tag="VYN80_002050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1179..1181),aa:Gly,seq:gcc)
     gene            complement(1236..1308)
                     /locus_tag="VYN80_002051"
     tRNA            complement(1236..1308)
                     /locus_tag="VYN80_002051"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1273..1275),aa:Thr,seq:tgt)
     gene            complement(1327..1408)
                     /locus_tag="VYN80_002052"
     tRNA            complement(1327..1408)
                     /locus_tag="VYN80_002052"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1372..1374),aa:Leu,seq:tag)
     gene            complement(1414..1486)
                     /locus_tag="VYN80_002053"
     tRNA            complement(1414..1486)
                     /locus_tag="VYN80_002053"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1451..1453),aa:Lys,seq:ttt)
     gene            complement(1511..1583)
                     /locus_tag="VYN80_002054"
     tRNA            complement(1511..1583)
                     /locus_tag="VYN80_002054"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1548..1550),aa:Asp,seq:gtc)
BASE COUNT          441 a          441 c          344 g          358 t
ORIGIN      
        1 atcgctctga acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc
       61 ttcaacccac tacagttgac aaagagcctt aagtttacgg atctgaagtt ttattgtctc
      121 taaaaacaaa aaagagagtt acaaactctc attaaaacgg agaataaggg attcgaaccc
      181 ttgcgccagt tacccgacct aacgatttag caaaccgtcc tcttcagcct cttgagtaat
      241 tctccaatta atgggcacga gtggactcga accaccgacc tcacgcttat caggcgtgcg
      301 ctctaaccac ctgagctacg cgcccaagtt aaaaaacttg gtaatttgaa caaagttcaa
      361 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtagtt ttaccactaa
      421 actacacccg cataaatact atcaataaaa tggcgcgaga cggaatcgaa ccgccgacac
      481 atggagcttc aatccattgc tctaccaact gagctaccga gccttattgc gggagcagga
      541 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccgagctgct ccatcccgcg
      601 ttaatataaa aggaggatgt gggattcgaa cccacgcacg cttttacacg cctgacggtt
      661 ttcaagaccg ttcccttcag ccggacttgg gtaatcctcc aatattcaaa tggaccttgt
      721 aggacttgaa cctacgacca ctcggttatg agccgagagc tctaaccagc tgagctaaag
      781 gtccgacaag atcattatag cggcgaaggg gatcgaaccc ccgacctccc gggtatgaac
      841 cggacgctct agccagctga gctacaccgc catgaatcgg gaagacagga ttcgaacctg
      901 cgacaccttg gtcccaaacc aagtactcta ccaagctgag ctacttcccg agttaaatag
      961 aaaaatgcac cctagaggag tcgaacctct aaccgcctga ttcgtagtca ggtactctat
     1021 ccagttgagc taagggtgct ccatattatg ccgaggaccg gaatcgaacc ggtacgatcg
     1081 ttaccaatcg caggatttta agtcctgtgc gtctgccagt tccgccaccc cggcctctct
     1141 aagcgaacga cgggattcga acccgcgacc cccaccttgg caaggtggtg ttctaccact
     1201 gaactacgtt cgcactgttt tcttctatct aaaaatgccg gctacatgac ttgaacacgc
     1261 gaccctctga ttacaaatca gatgctctac caactgagct aagccggctc atttgttata
     1321 tcttaatgcg ggttaaggga cttgaacccc cacgccgtta agcgccagat cctaaatctg
     1381 gtgcgtctgc caattccgcc aaacccgcat atatgacccg tactgggctc gaaccagtga
     1441 cccattgatt aaaagtcaat tgctctacca actgagctaa cgagtctaaa ataacttgcg
     1501 ttaccttaaa cggtcccgac gggaatcgaa cccgcgatct tcgccgtgac agggcgacgt
     1561 gataaccgct acactacggg acct
//
LOCUS       DAVLWA010000157         1536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLWA010000157 DAVLWA010000000
VERSION     DAVLWA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.157"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..695
                     /locus_tag="VYN80_002055"
                     /pseudo
     CDS             1..695
                     /locus_tag="VYN80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(730..1536)
                     /locus_tag="VYN80_002056"
                     /pseudo
     CDS             complement(730..1536)
                     /locus_tag="VYN80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          484 a          261 c          307 g          484 t
ORIGIN      
        1 agtaaaagga acaaaaccaa gaaaggttga tagagataga cttaaaaact atcttactga
       61 caatccagac gcttatttga ctgaaatagc ttctgaattt gcctgtcatc caactaccat
      121 ccactatgcg ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac
      181 aagacccaga aaaagtagcc ttatttctta aaaattttaa tagtttaaag cacctagcac
      241 ctgtttagat tgatgaaaca ggattcgata cttattttta tcgagaatat ggtcgctcat
      301 taaaaggtca gttaataaga ggtaaagtat ctggaagaag atatcagagg atttctttgg
      361 ttgcaggtct aacaaatggt gagttaatcg ctccaatgac ttacgaagag atggtgacga
      421 gcgacttttt tgaagcttgg tttcagaagt ttctcttacc aacattaacc acaccatcgg
      481 ttattattat ggataatgca agattccata gaatgggtaa gttagaactt ttatgcgagg
      541 agtttgggca taaactttta cctcttcctc cctactcacc tgagtacaat cctattgaga
      601 aaacatgggc ttatatcaaa aagaacctca aaaaggtatt accaagttgc aatacctttt
      661 acgaggctct tttctcttgt tcttgtttca attgactata ttagaggcga gacatttttc
      721 ggttctttgt caactgtagt gggttgaaga aagcgaagat ctagaaagga caaatttcgt
      781 cctttctttt ttgaagtttt caaagttcct aaaaccaaag gcattgtgct tgataagttt
      841 gatgagatta ttggtggctt ccagtttggc gttggaataa ggtaattgaa gggcgttgac
      901 gattttctct ttatctttga ggaaggtttt aaacaaagtc tgaaacagag gtggaaaagc
      961 aagagctgat agagattata gtggtgtttc aagtcttcgg aatagctcaa aagtttatct
     1021 agaatttctt tattagtcaa gtgcatacga aaagtagggc gataaaatcg tttatcactc
     1081 agtttctgac tatcttgttg aatgagcttc cagtagcgct tgatagcctt gtattcatgg
     1141 gatttcggat gatggcttgt gttctgctct caagaacagt tatgatattg agtttatcaa
     1201 agtcctgagc aataaagctc atctccatct cccgattgaa acagtcactc cccggactgt
     1261 ttcaacgtcc taggacataa tctcaggaag acgcgaaaaa tcatgctcaa agtgaaaatt
     1321 attgatcttg cgaatgacag ttgaagttga aatagacaac tgatgatcaa tgtcggtcat
     1381 agaaatcttt tcaattaact tttgagcaat cttttggttg atgatacaag gaatttgatg
     1441 attcttcttg acgatagaag tctcagcgag ctccattttt gagcaatgat agcacttaaa
     1501 acggcctttt ctaagaagaa ttctagtttg aatttt
//
LOCUS       DAVLWA010000158         1501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLWA010000158 DAVLWA010000000
VERSION     DAVLWA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.158"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            143..1339
                     /gene="tuf"
                     /locus_tag="VYN80_002057"
     CDS             143..1339
                     /gene="tuf"
                     /locus_tag="VYN80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1541884.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAVLVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          465 a          317 c          308 g          411 t
ORIGIN      
        1 taaattttta ccaagaataa ttcacaaaaa cgttgtaaaa cacttgcaat ttagctgaaa
       61 tttgataaaa tagtaaggaa agttagactg tattgcctac tgtctatcta taaaatatat
      121 tttattggag gcttttactc aaatggcaaa agaaaaatac gatcgtagta aaccacacgt
      181 taacattggt actatcggac acgttgacca cggtaaaact accctaactg cagctatcac
      241 aactgttttg gcacgtcgct tgccttcatc agttaaccaa cctaaagact atgcgtctat
      301 cgatgctgct ccagaagaac gcgaacgcgg tatcactatc aacactgcgc acgttgagta
      361 cgaaactgaa aaacgtcact acgctcacat cgacgctcca ggacacgcgg actacgttaa
      421 aaacatgatc actggtgctg ctcaaatgga cggagctgtc cttgtagtag cttcaactga
      481 cggaccaatg ccacaaactc gtgagcacat ccttctttca cgtcaggttg gtgttaaaca
      541 ccttatcgtc ttcatgaaca aagttgactt ggttgacgac gaagaattgc ttgaattggt
      601 tgaaatggaa atccgtgacc tattgtcaga atacgacttc ccaggtgacg atcttccagt
      661 tatccaaggt tcagcactta aagctcttga aggtgactct aaatacgaag acatcgttat
      721 ggaattgatg aacacagttg atgagtatat cccagaacca gaacgtgaca ctgacaaacc
      781 attgcttctt ccagtcgagg acgtattctc aatcactgga cgtggtacag ttgcttcagg
      841 acgtatcgac cgtggtatcg ttaaagtcaa cgacgaaatc gaaatcgttg gtatcaaaga
      901 agaaactcaa aaagcagttg ttactggtgt tgaaatgttc cgtaaacaac ttgacgaagg
      961 tcttgctgga gataacgtag gtgtccttct tcgtggtgtt caacgtgatg aaatcgaacg
     1021 tggacaagtt atcgctaaac caggttcaat caatccacac actaaattca aaggtgaagt
     1081 ctacatcctt actaaagaag aaggtggacg tcacactcca ttcttcaaca actaccgtcc
     1141 acaattctac ttccgtacta ctgacgttac aggttcaatc gaacttccag caggtactga
     1201 aatggtaatg cctggtgata acgtgacaat cgacgttgag ttgattcatc caatcgccgt
     1261 agaacaaggt actacattct ctatccgtga gggtggacgt actgttggtt caggtatggt
     1321 tacagaaatc gaagcttaat tcgatttagt tcccagaaga acaattattt aagtcagaca
     1381 ctaaaagaat cttgcttggc aaggttcttt tttggctctt tgtcaactgt agtgggttga
     1441 aaaaaaacta agctcgagaa aggacaaatt tcgtcctttc ttttttgatg ttcagggcga
     1501 t
//
LOCUS       DAVLWA010000159         1498 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLWA010000159 DAVLWA010000000
VERSION     DAVLWA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.159"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            13..466
                     /locus_tag="VYN80_002058"
                     /pseudo
     CDS             13..466
                     /locus_tag="VYN80_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1143..1403)
                     /locus_tag="VYN80_002059"
     CDS             complement(1143..1403)
                     /locus_tag="VYN80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541885.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          508 a          281 c          257 g          452 t
ORIGIN      
        1 tatagtagtt ctatgaatga tgaagcaagt aaacaactaa ctgatgcacg atttaagctc
       61 cttgttggtg ttcagcgtac cacttttgaa gagatgttag ctgtattaaa aacagcttat
      121 caaaaaagtc ggacaagctg gtaaaatctt ggctgacagt ggttatcaag ggcccatgaa
      181 gatatatcct caagcacaaa ctccacgtaa atccagcaaa ctcaagtcgc taatagctga
      241 agataaagcc tataaccatg cgctatctaa ggagagaagc aaggttgaga acatctttgc
      301 caaagtagaa acgtttaaaa tgttttcaac aacctatcga aatcatcgta aacgcttcgg
      361 attacgaatg aatttgattg ctggtattat caatcatgaa ctaggatttt ggtttcgcag
      421 gaagtctatt atatatgcac agacatatga aatctttttg agatagcagt tatgatatat
      481 ttatccattc acaacatctt ctgtactaat actacaatat tggtacattt tgattacaca
      541 atcgtttcaa aaaatatgat ggaagcctga gtattttata gtaattccct tcctatcaac
      601 tagatcaacc agttgagtgt tgaatgttga aacaccctag cctcagcaaa ggactatgat
      661 cagcatttcc tagccaatag gacgctccaa atcacctatt ctaagtccat cactagcttg
      721 gtcagtaaga tacagactgc aaggcgagac tgacgtggtt gatgagattt tggaagagta
      781 ttacaatcat agattcacca gtagacttga ccgcagattt aactatagta tattgaaact
      841 agaatagtac acctctactt ctaaaacatt gttagaaatc gatttgactg tcctgatcaa
      901 tttgtcctgt tcttttttca tttcactata ctaaaaaacc acctgaaaca aatcaagtga
      961 ctagaggata aaaacaaaga ctgggcaaaa agttttgaaa agcaaaaaac gcataatatc
     1021 aggtcttgaa aaaccttgac gtttgattgt gggaagattg acttcatttt ctcctgaaat
     1081 tgaagttttt tctagtttcc agatatagac gaattcaata tagccatctc gctacaatta
     1141 ttttaaccat cgagaaaatt tttcagaatt tcaaaaattt cattttgatc caatgaaatg
     1201 tcatcatttt gaagaatagt tgacttcaca ggctctaatt ctatcacaaa gcggtgtatg
     1261 tctgctttta ccgcactact tgatgttata gccgataact tttcatctcc tagcaaggaa
     1321 gctgtcaaac gggtaatgtc attcaaatgc tttttaatgg acttacttaa accttgagca
     1381 cctgtagcag agcgttccct catatccagc caagctttag ccttaaaaat aattaatact
     1441 caatgaaaat caaagagcaa actaggaagc tagctgcagg ctgctcaaaa cactgttt
//
LOCUS       DAVLWA010000160         1474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLWA010000160 DAVLWA010000000
VERSION     DAVLWA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.160"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(64..628)
                     /locus_tag="VYN80_002060"
                     /pseudo
     CDS             complement(64..628)
                     /locus_tag="VYN80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(704..916)
                     /locus_tag="VYN80_002061"
     CDS             complement(704..916)
                     /locus_tag="VYN80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541886.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(925..1230)
                     /locus_tag="VYN80_002062"
     CDS             complement(925..1230)
                     /locus_tag="VYN80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEV1541887.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1217..1411)
                     /locus_tag="VYN80_002063"
     CDS             complement(1217..1411)
                     /locus_tag="VYN80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1541888.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          410 a          316 c          274 g          474 t
ORIGIN      
        1 ggaaaattga tttatagaaa tgttttagca gtcgatgcgt actgttatag attcaacgca
       61 ctataattga tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc
      121 tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt aaactgagct taggctaaaa
      181 gagtccactg gactctttta ctccgtccca taaccaatga tttaatggcg cattcagcta
      241 gattattgga aaggaccaga tgtccgtctt tcaaaatagt cttaaaggtt tctttatact
      301 tgaggctata cttgaggctg tattcaattg cccttcctag ttttgaaccc gataaaactg
      361 actgacgccg gcaccaagca aagaaatctt ccattagggg ttggagatgt tcttgacgtt
      421 tctgtagtcg ttcatcagct ggcaaagcct cccagtttct ttccaaggaa aataactgat
      481 tacaataagc taaaccttta gctcctaagg atgatttatt cgcttgcttg ggggtcgctt
      541 caaaaaactt ccttctcaca tgtacccaac atccaacaag ttcagcttct tccagttgac
      601 aataagcttc tagcggttcg cagtttacca agcttagcgt cgccttgctc ctaaaccttg
      661 gactgctatc gcataggcag aactagagga ctaaagtcct aagttactgc cgcaacatat
      721 cacaatgcac atagccagaa taatctccta ggaattcttg cactaccgaa ccactccgac
      781 gctgatcatg gtggtacagc gtgattgctt gattctcagc tttcccagac aaaaaggtcc
      841 aatagtaggt cagatggcta tcattctcta gaacccgata ggaggtttca tctgcatgaa
      901 gtaagggctg aatcaatagt ctcgttataa aggggcttta ggaataggag cttttacaat
      961 tttatcactt ggattattct tactgcatgt ttggcatttg taagcgtgtt ggatatgatc
     1021 cactcttttt aattgcgcag gaataaagac caattcttgt cgttgaaggc tcgctccaat
     1081 ctcttttaaa tcgtcctgac aatcagggca aatgctctct tctacttgat gatgaacttc
     1141 ttctgaatca aattgggcaa gaatagcttg acgtttccct ttagatttct tacgtttgta
     1201 ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt ctcagatgga gactcttctt
     1261 caaaaaggtt gagttgtcca aaagggcaaa cacttttctc agaggatttt ccagagagct
     1321 tttgcgttag ataagccact tgttcacaca gaagggaaag ttcattagtg agactatcaa
     1381 ttatagcact ctgttgttga atgattttca atcttctccc atagactttc ctccttattt
     1441 cctaacttta ttatatagtg tattgaatct ataa
//
LOCUS       DAVLWA010000161         1469 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLWA010000161 DAVLWA010000000
VERSION     DAVLWA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.161"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            22..1017
                     /locus_tag="VYN80_002064"
     CDS             22..1017
                     /locus_tag="VYN80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541889.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPF
                     VSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            1007..>1469
                     /locus_tag="VYN80_002065"
     CDS             1007..>1469
                     /locus_tag="VYN80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541890.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT          496 a          208 c          283 g          482 t
ORIGIN      
        1 atgatagaat aaggaaaaaa catgaatgtc aaaaagataa tgtcaatttt tcaatccttt
       61 tatgttgatg tcagtattga ggaactgact ttgactttac caatcagttt tgtaaaaagg
      121 tttgagtata ctcaaatgac ttttcataag gaatcatttt tattgattaa agaaaagaga
      181 agggggagtt tgagttcatt tgttactcag gctcgcacta tgggtgaaaa agccaatatg
      241 gatgttgttt tggtgttttc gaagttatca gacagtgaaa aaaagcaatt acttcaagct
      301 agagttccgt ttgtagactt taagggaaac ctcttcttcc ctccattggg actagtactg
      361 aatgcgaatg atactgaagt ccctaaggaa ttaacaccta gcgaacaatt aacgtggatt
      421 gcctttttat tgacaaaagg tcaaaaagta gtagatgttg atttgctttc acaagtcact
      481 ggacttccaa actcaacaat ttataggtgt ttgaggactt ttaaagcttt atattggtta
      541 aacaagcaaa ataagcttta cacatatacg gtgtcaaaga aagaattatt cttaaaatcc
      601 gtgtcatgtt tatttaatcc catcaaaaaa cggattttat tgccagatgg cgatataaag
      661 cagataaaat ctgtttctaa ccttctatat ggtggtgctt atgctttgtc gcattcaact
      721 tttttagctg aaacggatga aaatactagc tatgtcatat ggcagagaaa attcaatcag
      781 ttatccttgc cactttctca gcatgtttta aaaggaaaga tgctagagat atggaaatat
      841 cgtccttttg tatctgagtt ttggaatgat tttaaaaata atcatgataa acaatttgta
      901 gatccgattt ctctttattt gaccttaaaa gatgatgatg acccacgtat agaggaagag
      961 agtgaagcac tagaaaatat gatattacag tatctgggag aagatgatgc cagctaatac
     1021 gaaagttatt tttcaagaaa tgtttgcgga ttttcagaac tattatgttc tgattggggg
     1081 aactgctacc tctatcgtat tggattcgca aggatttaaa agtcgcacaa caaaagatta
     1141 tgatatggtc atcatttatg aagtaaaaaa taaggaattt tatactacct tgaatcattt
     1201 tttagaattg ggagagtatc aaggaagtca gaaagatgag aaagcgcagc tttttcgatt
     1261 tacaacaact aatcctgagt ttccttctat gattgaacta tttagtatct taccagaata
     1321 tccattaaag aaggacggtc gagaaattcc cttacatttt gaccaagatg ctagtttatc
     1381 agccttatta ttggatgaag attattataa tatattggtg catgaaaaag aaaccattca
     1441 ggggtattcg gtattgagta attgtggtt
//
LOCUS       DAVLWA010000162         1459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLWA010000162 DAVLWA010000000
VERSION     DAVLWA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.162"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(1..505)
                     /locus_tag="VYN80_002066"
                     /pseudo
     CDS             complement(1..505)
                     /locus_tag="VYN80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            761..1459
                     /locus_tag="VYN80_002067"
                     /pseudo
     CDS             761..1459
                     /locus_tag="VYN80_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          453 a          280 c          275 g          451 t
ORIGIN      
        1 tttgaacaac ttcatatcat gactatagtt cacagtgata tccaaagaaa caattctccc
       61 ttgacttgtg acaatcgctt gagccttcat agcgtggcat ttctttttac cagaatcatt
      121 cgctaattct ttttttaggg cgattgattt gtacttccgt cgcatcaatc attaccgtgt
      181 cctcagaact gagaggagtt cttgaaatcg taaaaccact ttgaacaaga gttacttcaa
      241 cccattggct ccgacggatt aagttgcttt cgtgaatacc aagatcagcc gcaatttctt
      301 cataagttcg atattctcgc acatattgaa gagtggccat aagaaggtct tctaggctta
      361 atttaggttt tcgtccacct tttgcgtgtt taagttgata agctgttttt aatacagcta
      421 acatctcttc aaaagtcgtg cgttgaacac caacaagacg cttaaatcgt gcatcagtta
      481 gttgtttact tgcttcatca ttcatagaac tattatacca tattttgttt cgcaggaagt
      541 ctattgaaaa atctccagat tagtgaactc acggatagtt cctaatcagg agatttctta
      601 tttgcacttt tcttgtacaa ctttagtcca tggtaaataa gcctctaaaa cctctttgtt
      661 tacgagagtt tcctcgtttg gaagacattc tagaagatag gatagatatt tctcgctatt
      721 tatactaaac taaaatcaaa aagcattata taatagtgat atgaaatcaa ctaaaaaaga
      781 aatccaaacc atcaaaacac ttttaaaaga ctctcgtaca gctaaatatc ataaacgcct
      841 tcaaatcgtt ctatttcgtc tgatgggcaa atcttataaa gagattatag aacttttaga
      901 ctgtaatcaa acaacgattt ggcgaaatgt aaaaaaatat gaggagttcg gactcgactc
      961 cctccttcaa gaaacacgtg gtggtcgtaa ccatgcatat atgacagttg aggaagagaa
     1021 agtctttctt gcccgccatt tgaaggctgc agaggcagga gaatttgtta caattgatgc
     1081 cttatttcag gcttataaaa aggagttagg tcgttcctac acacgtgatg ccttctatca
     1141 actgttgaag cgccatggtt ggcgaaatat tacgccacgt ccagaacatc ctatcaagaa
     1201 gaaaagaaag cgctttaaaa ccagtagatg ttttcataag gttcgcttga tgtaccaaga
     1261 tgaagctggt ttcggtagaa tcagtaaact gggatcttgt tgggctccaa taggagtagg
     1321 tccacatgtc catagtcact atatacgaga atttcgctat tgttatggag ctgttgatgc
     1381 ccatattgga agatgtcatg aatcaactcc aagatgtcat acaaggactg gagaaggagg
     1441 tgataaagtc catcgttaa
//
LOCUS       DAVLWA010000163         1446 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLWA010000163 DAVLWA010000000
VERSION     DAVLWA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.163"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..1042)
                     /locus_tag="VYN80_002068"
     CDS             complement(<1..1042)
                     /locus_tag="VYN80_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1541891.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIE"
     gene            complement(1108..1446)
                     /locus_tag="VYN80_002069"
     CDS             complement(1108..1446)
                     /locus_tag="VYN80_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541892.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
BASE COUNT          507 a          267 c          198 g          474 t
ORIGIN      
        1 gttctatata taagaaatct aagtttttta aggcagcaac tatctgtaga tactgaccat
       61 ctgaatcatt aacttctcta tttttagtag agttactttc aattccaagg aactcaatcc
      121 ttctccagtc ttttactgta tcccatggta ctaaattctg tatctgtatg ttaatactag
      181 attgactact ctttgtttct tgtaaaaaaa ctcctgagac aaggatgata atcgaaataa
      241 tagcaagcca tacgacaaaa ataagcttgt tttttgcctt gtttttaata atttcaatag
      301 gcttttcaat ctgaatcgtc aaccaaaata aaacaaaagt aataagatca atgatttgaa
      361 atagtataaa attcgttaaa agcagagaga aaaataactt agaactatag gtgaagaaac
      421 cattacccaa aaaagaactg taagaaatca tcaataaagc catcataatc agttcaaaaa
      481 taagagaaat gccatactct cttcttaagt cgtatatagg caagcccaaa gaacgtcgta
      541 tcactccttc tttgatctgc ctcgtcctaa caacaaataa aacaactaat aaagttaaat
      601 aaattgaacc cattaacagt attcttaaag ttccagtgaa ttgtaatatt cctcctatat
      661 accaaggata agccatatag actgtttgta gtcccgtcat cgtaagtggt ttcaaactgt
      721 ctacatccat tttcccaagt gtgtaataca tccctactat tggtgctgac tttaattgtt
      781 cattatttac atcatcaaaa tttacatact tcaactgacc cgaactctga acgattggct
      841 tgtaaatggt aaccttttcc gtttttgata agtcacgaac catttcgtat atttcgttat
      901 tagaaagatt actttttcct tggatagaaa aagctccatc aattggtaaa ggtatgggga
      961 tttcggtatc actaatagtg accagaccaa ttgtagcaat caaacaaaag aagaatccaa
     1021 tgatacataa ttttaatttc ataagcaatt accatattac aagagaccca atcttataat
     1081 tgggtctctt gttttcataa attattttta aaatctataa taatcccaat atgcatttaa
     1141 gcgaacatcg ttccaagatt ttgttgcact tgaatatgcc catccataat cttttttatc
     1201 ctgagcttta actgttcccc aaaaattcgt tactgaagat tttgatccaa tattacttgg
     1261 agaactaaca aagtaattcg aataaccata gttatcattt acaccatatt cccatacatc
     1321 aataatggga cttggagcag ggtgtgccgt tactgcgtta acgacactag ctgtagtagc
     1381 aaagctaaaa ctgaaaacac ttgcaactag aaaaacagat gctttcttat taattctttt
     1441 tttcat
//
LOCUS       DAVLWA010000164         1417 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLWA010000164 DAVLWA010000000
VERSION     DAVLWA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.164"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            118..1296
                     /locus_tag="VYN80_002070"
     CDS             118..1296
                     /locus_tag="VYN80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1541893.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          532 a          253 c          206 g          426 t
ORIGIN      
        1 tatataataa aaagagacca gtaagtaatg aaaagcttaa aaaaattaac atctacattt
       61 atccatagca aaaaatcata ggattttaag aatctgacaa ctaggaagaa aactaatatg
      121 accttatcta agaaacaatt acaactaaga gctaaaatac tggaaacagt ttacacatta
      181 ggtcccattt cacgaattga aatcgctact aaaacaggaa taacacctgc aacaactagc
      241 agtatcacaa atgatctgat taaagaaaat attctcttag aacttggtga agacgaacac
      301 gatacaagcg tagggagaaa aaaaatcctg ctagacattc aagctaaacg attttactac
      361 attggttgtg aactatctga aaaacatttc acttttgctt taggagataa tctaggaaat
      421 atcttaaagg aagagaaaga aattgttact aaacagttaa tccaagaaaa gggaaatcaa
      481 ctcattaacc agacactaaa gcaattctta aataactgct ctgattatga aatagaagca
      541 attggaatcg cgctacctgg tcgctattta gataattata aaataacaac aaacaatcct
      601 ttatggcaac atatcgactt agaaatgatt caatcccact ttgataagcc tttgtttttc
      661 tctaacaatg ttaactgtat ggctataggt aaacgcctat ttagtcgcca acagaatgac
      721 cctaattttg cttattttca ttttgctaga ggtatgcact gttcttatat ctacgacgga
      781 aacatttatg ggaaaggaaa tctaatgatt ggagaaatag ggcatacagt tgtttcatct
      841 gagggagaag aatgtagctg cgggcgcaag ggatgcttac aaacttttgc tggagaatct
      901 tggttaatca aaaaatcaaa aattctatat catcaatctc cctattctct acttcctagt
      961 ttagtcaaaa atgccgatga tattgatatc caagttattt taaccgctta tcaattaggt
     1021 gacactggaa taatcacact tatccatcaa gccttactct atcttagtca aaccatttta
     1081 aatatcagta tgatgattga ctctcaaaaa atctacctac atagtccttt actaacaaat
     1141 caacacatca tccaaaaact atatagtgaa atgaactaca aacccaaact gctctataat
     1201 cgcctacctg aagtcattat tgaaccttac aatgatttta cagccgcaca ctcagctatt
     1261 gcactctgtt tgtatcatac aattctacat tcttaatttt ctattacatt taataaatag
     1321 gctaaaaaga gggagtatat cataaaaagc gaataaacct gttttctgaa aatcagaatt
     1381 ctgttttgtt cgctttcttt atatttggaa ttatact
//
LOCUS       DAVLWA010000165         1379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLWA010000165 DAVLWA010000000
VERSION     DAVLWA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.165"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(151..339)
                     /locus_tag="VYN80_002071"
     CDS             complement(151..339)
                     /locus_tag="VYN80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541894.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(299..481)
                     /locus_tag="VYN80_002072"
     CDS             complement(299..481)
                     /locus_tag="VYN80_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541895.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(483..1262)
                     /locus_tag="VYN80_002073"
     CDS             complement(483..1262)
                     /locus_tag="VYN80_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541896.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          458 a          294 c          201 g          426 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagctc tctgcgactt ggattaccat ctagataagt ttaaagagcc aatccaatat
      181 cttaaataat atcttgtaaa acagcaattg aactgcaaag gaaatgatca tccaaaagaa
      241 tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagaccaa aaaaaatttc
      301 attctctctc ctctggccta atgagccacc gagccgtatc cattagctta tctgcaataa
      361 aaagttttcc cagaccaaca acgacatggt cagctttctt tatcgttttc atcactttta
      421 caccttgcac ggtcaaaaag taattaccat aaattttatc aaaagctttg ataggattca
      481 tattactctc ctttaatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat
      541 tgaacttcac tatagttgac tagcttggat aattctttct gcaagctttc ccccattggt
      601 ttcatagttg caaaggttaa accacctgca atgataccac ccaagatagg aataaatttc
      661 cctattcctt tggccagccc tcccttggta agattcacac caaatatttt taagactttt
      721 ttcaaaatag ggtaccaaag cgtctttgtt aaagctttat taggcactgt tttcattacc
      781 tgtttggcaa ttgttatacc accagcacat agcaaagcag cggttccatt cacccctaac
      841 attacgccta gataaagcaa gagggtattt tgagcatctt cactcaactc ctcgcgtgaa
      901 gcccaaagat cctcataacc ataaatataa cctaattctt gagccaattt cagagagaaa
      961 gcataaaatt gagccacatc agctggaatg gtaattgcca tagcaagccc tccagctaat
     1021 cctgccaaaa cagaagtccc actcgctaat aatacattgt cccgaataca agcattagct
     1081 acacgatcta atatttcttg agatagaaga gtcgttgtac cttgttccaa taaggtagga
     1141 atatcttttg gatccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
     1201 ttaacaacag gtagtttcac cacttgttgc aatacttgca tagctaagtc ttgttcagcc
     1261 atatacaaac tccttatttc ttttttacac cttatcatat tatatttcct acaaaaaaag
     1321 cattttctaa ttagataaca ttaaaaaata cagaacccct tttttatagt aaaatgaaa
//
LOCUS       DAVLWA010000166         1308 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLWA010000166 DAVLWA010000000
VERSION     DAVLWA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.166"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..156
                     /locus_tag="VYN80_002074"
     CDS             <1..156
                     /locus_tag="VYN80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541897.1"
                     /translation="LGIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDNEYREMMKN
                     KKMDQER"
     gene            230..403
                     /locus_tag="VYN80_002075"
     CDS             230..403
                     /locus_tag="VYN80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541898.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            589..1116
                     /locus_tag="VYN80_002076"
     CDS             589..1116
                     /locus_tag="VYN80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV1541899.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1243..>1308
                     /locus_tag="VYN80_002077"
     CDS             1243..>1308
                     /locus_tag="VYN80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1541900.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRI"
BASE COUNT          690 a          175 c          203 g          240 t
ORIGIN      
        1 cttggtatag atttatccat agaaatagat aagctaataa aacagggaga aaaaccaagt
       61 gtaatagcac agattaaaaa gttccaagac caagttaata aagataatga atacagagaa
      121 atgatgaaaa acaagaaaat ggatcaagaa agataagacc tggtaaaaat atcttatcca
      181 agctataata tgaccaaaca actaaagtca gatctaggag gtaatagaga tgaataaaag
      241 gcaagagcta atagatgaac tcataaaagc aaatgaagat ggaacataca aaacctataa
      301 aagcacagaa gaaataaaag caatgaacaa tgaagaaata cagattatat attccaacat
      361 gaaaaactat ctatcagaca aaagaacaca cataaactat taaaggtgga aaggtagtgt
      421 aagctatttt taaaagtata aaaggtacaa agacctaaga aatatataaa aacatcaaaa
      481 taaagtattc caccaccaag aacaataaaa taaaaacaag caaagggaag tatagaaaaa
      541 ttaaaaccta tacttccctt ttttaattca aacaaaggag agaaaagcat gataaacgaa
      601 gaaataagca aggaagcagg tcaagcagca caaaccataa tatcatacac aataaaggca
      661 acaaaagaat caatcaattt agaaaaagaa ataagaaaaa agatgaatga aactttagaa
      721 aaagcaaatg gaaacttaaa aagtcttatg ggcgatgaaa tgaaaataaa agatctctac
      781 aagaaaggac aactagaaaa tataagcata gatcaaatcg acctcaaaga cttaaaaaaa
      841 gaactaaaca aacttggagt aagtttctca gtaatgaaaa acaaagaaag caaaaactat
      901 gaaatattct tccaagccaa agacataaaa gtaatggaat atgcctttaa gcaagtcata
      961 gccaaggaaa ataaaaaaga aaaagaaagt atcctaaaac aaataaagaa atacaaagac
     1021 ctatccaaaa acaaagataa gacaaaagaa aaaggaaaaa ggaaagtaaa agaaaaagta
     1081 aaaccaaaca aaaaagatat gaccagagaa atctaaaggg agtaacgaaa agttacaccc
     1141 ttaaataacc ataataacaa gacttccgaa aaacagaagt ccagaattcc gaaaatcgga
     1201 aatcaagaat ttcgattatc gaaaatcaag aaaggagcag atatggcaac aaacaaaaga
     1261 tactattgga taaaattaaa agaagaattt ttcacagaca aaaggata
//
LOCUS       DAVLWA010000167         1292 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLWA010000167 DAVLWA010000000
VERSION     DAVLWA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.167"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            137..>1292
                     /locus_tag="VYN80_002078"
     CDS             137..>1292
                     /locus_tag="VYN80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV1541901.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSE"
BASE COUNT          485 a          154 c          231 g          422 t
ORIGIN      
        1 aaattataat ttatctataa ctcaaataaa gaattctatt aaagaaaatt ccttagtttt
       61 gtttgttggt gcaggaatat cagctaattc taatctacca acttggggag aattgattca
      121 atcattaaaa aaagaattga acataccaga agaaagaaca gatagtccac tgagaattgc
      181 tcagtattat tatgatactt ttgggaaaaa tcagtacaca aagaagatag aagaaatttt
      241 ctttaagaag ggattgagta aaccaaatga gttacataaa ttaatagaaa aaattgcacc
      301 aaaacatatt attacgacaa actatgattc acttctggag agtcaatttg aaagtggctt
      361 gttaaagtac aatgtagtcg ctgaggataa agacatccct tatacgagtt cggaaagata
      421 cttgattaaa atgcatggtg acttcagtaa gaaaaatatt gttttgaaag aagatgatta
      481 tttagactat catttgaact tcccaatgat ttctacttta atccagtcat tgataatgaa
      541 tcatactcta ttgtttgtgg ggtattctct aagtgattcg acttttaatt caatttttag
      601 aatgattcaa aatacattta aattggatgc taaaaatgct tatttttata ctcccgagga
      661 accgtctatg atcattcgag agtattataa gaagcaagga attttcatta tctctaatga
      721 agagaatcta ggtcaggaga catcagaaaa acagaacaaa ttgtattgca ggacaaaaga
      781 ttttttagag gttctatcag aaaaccgaag tcaagatgta aataatgctg atgatttatg
      841 gaatcaactg gcttttttag atagacttag ttttattgat gcgaaagatt tttctagata
      901 ttctgattta aagaaaaggg ctttaaactg ggatgatgag tattgctggt ttggaaatga
      961 tcaattaaga ttcgaaattg atggtcatga agaattacga attatggtat ctaaaaaatc
     1021 tttattaaat cgctttctag atatggagat tggtgagcct agggatttaa aggggaatcg
     1081 attcttaagt aaggctttta agttgtatga agaaaaacaa tattctttag caaaagcaaa
     1141 atttagagaa ctagctaata tagcatttcg tcagaaagac tattttaatt tcttagtttg
     1201 tgaatttaat tttcagcaaa ttcaaataat tgatagatac gaaaaaacac ctagttatgc
     1261 agagccactt tatgatggag aattaagtga at
//
LOCUS       DAVLWA010000168         1218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLWA010000168 DAVLWA010000000
VERSION     DAVLWA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.168"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            176..799
                     /locus_tag="VYN80_002079"
     CDS             176..799
                     /locus_tag="VYN80_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541902.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            796..>1218
                     /locus_tag="VYN80_002080"
     CDS             796..>1218
                     /locus_tag="VYN80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541903.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKE"
BASE COUNT          454 a          153 c          237 g          374 t
ORIGIN      
        1 tcaaatattt tatgatgact attacaatgg ttattctatt tacatttctt atgacagggc
       61 aaatagagtt gctatatatt ggagcggctt ctctgctagt tttgatgttg tcaatcataa
      121 tgagttttag aaaattatct gaaagctaca ctaaaatttt aaaaggagat gaatcatgac
      181 aattgacctt ttgaacgttt caaaaagttt cggctcaaag aagatcttta cagacttaaa
      241 tttaatattt gaatctggaa aaagctatgc attgattgga ggttctggct ccggtaaaag
      301 tacccttctt aatattatag gaagattaga aaaaattgac agtgggaatg ttttagttga
      361 taaacaagat atttggaaga taaaagaaag aacttttttt aaaaatactg ttggatatgt
      421 atttcagaat tattctttaa tagataacaa aacagtgtat gataatctga gccttatcac
      481 taaagacaaa aaaacaataa ctgacgtact tgaaaaagta ggactgtcaa gtgactattt
      541 acaccaaaaa atatatgaat tgtctggagg gcaggctcaa cgtgtagcta ttgccagaat
      601 gttaatgaaa ccacgtaaaa ttatcttagc agatgaacct acaggagctt tagatggtga
      661 gattggaaaa gaaattattc gtttactatt gaatgaaaca gctgaagata aatatgtcat
      721 tatagcgaca catgatccag ctgtctataa cgaagttgat gtgataattg acatgaaaga
      781 tatagggtat aaggtatgaa aaagaaaatt tatattgctt tgatctttgt aactggggta
      841 ctcgccattt tcttttttgg aaaacaaatg attacaaaag aaaatatcaa taagccaacg
      901 gtagagttaa caatctatac gctttcttct agtgatacag aaaaatggaa taaagtgaga
      961 caagtagaaa cggaagaagc tatatacttc ataactgtga aagaagtatc atcttcagaa
     1021 gaagtatttt caaacatcat tgcaaatgga gctgctacgg ggttcggagt tcgtgaagaa
     1081 gaagtgaaga aattcaataa tggtttagga gacaccatag aggactctaa gcataataaa
     1141 ctaattgaaa tagaattttt tactttttct gatgatggcg cgggatttgt ggtagcaaac
     1201 tttgactacg gtaaagaa
//
LOCUS       DAVLWA010000169         1177 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLWA010000169 DAVLWA010000000
VERSION     DAVLWA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.169"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..849)
                     /locus_tag="VYN80_002081"
     CDS             complement(<1..849)
                     /locus_tag="VYN80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1541904.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
BASE COUNT          393 a          154 c          204 g          426 t
ORIGIN      
        1 taggtttgaa aaatcattaa tttctttttc gagtatttta actaatgcct cttctttggt
       61 tagtcgtaat aaagttattc ctttatcaat caaatcggag ttatgttgta gtattcccaa
      121 gcgtacttga gagaatccaa gaatatcgta tatttttaat tcttctgcta ataccaaaag
      181 ttgtagggtg attgtttcac attctttgaa taaatgatgt tgaaaatata cagtggagag
      241 attggctaaa aaagatgata aaaatgcttt tgtttctcgg aagtatttat atttctcgat
      301 ggttttcata attctatcaa taagaagggg aagattttca gaaggaaaga aaaagagaat
      361 ggttccaagc aatttcaaat cactaatgta ccatgtatcc ataggttcaa gatagtccca
      421 aatttcttca tacaaatctt tcaagacgtg gttgtttttg aatcctttag ttcgtaactc
      481 tgtgacaatt tttgtatgac gatagatatt ttgaatagga acatcgtgat gtgtcttaag
      541 atatttttga catttctcag tgagttcaac catttttcta gtatcttgaa aagtagacgg
      601 attttggctc tctacaataa tatctcgcct tttactagga tggtattcgt tgcatatata
      661 cttgaattct gccaagctca tatcaatttg ttcaagaaga aataccatgt tttcaaaact
      721 gggaataact ttaccgtgtt caatatttgt tagagttgtt cgatgtatga cttgtccaca
      781 tacatcttgt tgggtcaatc cttttgactt tcgaatttct ttaaaaattt gtccataatc
      841 ccatctcata ttgttcctcc taggtaaatg tgaatatttt ctacatttat aaaaaacgta
      901 gaaaaaggtg gtattattat atcaaaaagg agggaaaaag tcatgggctt taaaaaatat
      961 ttgaagaatt taccgaaaaa ctctggattt ttgatttgga gttggattca acttatctgg
     1021 tttgaaacat ggttttgggg ataaagaaat tagagtctat acataataag taaaggagga
     1081 atttgtatga tataccatcg tttagaataa gtctatattt aaaatagaag ttataaggaa
     1141 tcttcttgaa taaaaaatga aaggaaacag aaaaaat
//
LOCUS       DAVLWA010000170         1124 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLWA010000170 DAVLWA010000000
VERSION     DAVLWA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.170"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            57..>1124
                     /locus_tag="VYN80_002082"
     CDS             57..>1124
                     /locus_tag="VYN80_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541905.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEP
                     NEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGF
                     LRDYSGELRFCGDDIKKTSYLN"
BASE COUNT          389 a          141 c          202 g          392 t
ORIGIN      
        1 tattgctaat ttatgcagca actattaatg ctctggtgtt gaatgaatta attgcgatga
       61 atttagagcg gtttttgaaa ttgtcaatct accaaatgat tgtctggtgt gggataatat
      121 tccttgactg ggtagtgaaa aattatcagg ttgaagtgat ccaagagttt aatctagaga
      181 ttcgaaatag agttgccaca gacatctcta actctaccta tcaagaattt catagtaaat
      241 catcaggaac atatctttcg tggctaaata atgatgttca gactttaaat gatcaggctt
      301 ttaaacaact ttttttagta ataaaaggaa tttctggtac tatatttgca gttgtgactc
      361 ttaatcacta tcattggtca ttgactgtag ccaccttgtt ttcattaatg attatgctac
      421 ttgtaccaaa aatctttgca tcgaaaatgc gagaagttag tctaaattta actaaccaaa
      481 atgaagcttt tttaaaatct agtgagacta tattgaatgg atttgatgtg ttagcatcct
      541 tgaatctttt atatgtattg cctaagaaaa ttaaagaagc aggaatttta ttaaagatgg
      601 ttatacaaag aaagacaact gtagaaacgt tagcaggcgc tattagcttc tttctcaata
      661 ttttttttca gatatctctc gtttttttaa caggctatct tgcaataaaa ggaatagtga
      721 aaattggtac tattgaagca ataggagcac taacaggtgt tatttttaca gcgctaggtg
      781 aattaggagg tcaattatcc tctattattg gtacgaagcc tattttttta aaattgtatt
      841 caattaatcc aattgagtca aataaaatga atgatatcga accaaatgag gtgaatagag
      901 attttccgtt atatgaagca aaaaatattt gctataagta tggagataaa gaaatattaa
      961 aaaacttaaa tttttgtttt caacgtaatg aaaagtattt aattttaggt gaaagtggaa
     1021 gcgggaaatc tacattatta aaattattga atggcttttt gagagattat agtggagaat
     1081 tgcgattctg cggggatgat ataaaaaaaa cctcctattt aaat
//
LOCUS       DAVLWA010000171         1120 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLWA010000171 DAVLWA010000000
VERSION     DAVLWA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.171"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..673
                     /locus_tag="VYN80_002083"
     CDS             <1..673
                     /locus_tag="VYN80_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541906.1"
                     /translation="KSTLIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQS
                     PIIPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGG
                     QRQRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDIN
                     LTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGK
                     HHVSS"
BASE COUNT          463 a          185 c          176 g          296 t
ORIGIN      
        1 gaaatcaaca ctcattaaaa ccttgtctcg acttataaag ccattagagg gagaagtatt
       61 gcttgataat aaatcaatta attcttataa agaaaaagat ttagcaaaac acatagctat
      121 attacctcaa tctccaataa tccctgaatc aataacagta gctgatcttg taagccgtgg
      181 tcgtttcccc tacagaaagc cttttaagag tcttggaaaa gatgaccttg aaataataaa
      241 cagatcaatg gttaaggcca atgttgaaga tctagcaaat aacctagttg aagaactttc
      301 tgggggtcaa aggcaaagag tatggatagc tctagcccta gcccaagata caagtatcct
      361 acttttagat gagccaacta cttacttgga tatctcatat caaatagaac tattagacct
      421 cttgactgat ctaaaccaaa aatataagac aaccatttgc atgattttgc acgatataaa
      481 tctaacagca agatacgctg attacctatt tgcaattaaa gaaggtaaac ttgttgcaga
      541 gggaaagcct gaagatatac taaatgataa actagttaaa gatatcttta atcttgaagc
      601 aaaaattata cgtgacccta tttccaattc gcctctaatg attcctattg gcaagcacca
      661 tgttagctct taataattga atattggaat tcatttaaaa gtcgcaaatt ttgaactgac
      721 cctcaaaagt taggccaaaa aactaactaa aggaggtcag ttttttaatg gcaaatacag
      781 cacagaattt aagataaaat tagtaaaaga atatcatgaa tgaaaaatag gatatagaga
      841 ataacagcaa tattaaaaag atcaggactg ataataaaca aaaagaaagt acaacgacta
      901 gttcaaaagc taaaacttca agtaaaaagt tattcaagaa aatctagaaa atactcatcc
      961 tacaaaggac aggtaggaaa aatatcagac aacaaaataa aaagaaatat ttattaccga
     1021 aaaaaagggg aaatcgagtc aagtaccaac aggctcatct attccaaaaa gatatttact
     1081 cgatggaaaa actcctagaa ttagtgttac aaatattgac
//
LOCUS       DAVLWA010000172         1119 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLWA010000172 DAVLWA010000000
VERSION     DAVLWA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.172"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..1018
                     /locus_tag="VYN80_002084"
     CDS             <1..1018
                     /locus_tag="VYN80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1541907.1"
                     /translation="KFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFG
                     QTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNG
                     KANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWR
                     DMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSK
                     FWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDD
                     KTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNAC
                     KNAK"
     gene            1008..>1119
                     /locus_tag="VYN80_002085"
     CDS             1008..>1119
                     /locus_tag="VYN80_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1541908.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIR"
BASE COUNT          436 a          158 c          187 g          338 t
ORIGIN      
        1 caaatttttt ctaatagcta ttttagctat gtgtatagtt tttagcgctt gttcttctaa
       61 ttctgttaaa aatgaagaaa atacttctaa agagcatgcg cctgataaaa tagttttaga
      121 tcatgctttc ggtcaaacta tattagataa aaaacctgaa agagttgcaa ctattgcttg
      181 gggaaatcat gatgtagcat tagctttagg aatagttcct gttggatttt caaaagcaaa
      241 ttacggtgta agtgctgata aaggagtttt accatggaca gaagaaaaaa tcaaagaact
      301 aaatggtaaa gctaacctat ttgacgattt ggatggactt aactttgaag caatatcaaa
      361 ttctaaacca gatgttatct tagcaggtta ttctggtata actaaagaag attatgacac
      421 tctatcaaaa attgctcctg tagcagcata caaatctaaa ccttggcaaa ctttatggag
      481 agatatgatt aaaattgatt caaaagcctt aggtatggaa aaagaaggtg atgagttaat
      541 caaaaatact gaagctcgta tatccaaaga attagaaaaa catccagaaa tcaaaggaaa
      601 aatcaaagga aaaaaagtat tatttactat gattaatgct gcagatacat caaaattctg
      661 gatttatact agcaaagatc caagagcaaa ttatttaaca gatttaggtc tagttttccc
      721 tgaatcatta aaagaatttg agagtgaaga tagttttgca aaggaaattt ctgcagaaga
      781 agcaaataag ataaatgatg ctgatgtaat cataacttat ggtgatgata aaactcttga
      841 agctttacaa aaagatcctc ttttaggtaa aataaatgca attaaaaatg gtgccgttgc
      901 tgtaattcca gataatacac cgttagcagc ctcatgcact ccaacaccac tttcaataaa
      961 ctatactatt gaagaatacc taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa
     1021 aacaaataaa cctaggcata atttttgtaa tctgcctagg tcttcttatt acaatatttt
     1081 tgtcattaaa gcttggaaca aaagaaatta atatcagag
//
LOCUS       DAVLWA010000173         1107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLWA010000173 DAVLWA010000000
VERSION     DAVLWA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.173"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..440)
                     /locus_tag="VYN80_002086"
     CDS             complement(<1..440)
                     /locus_tag="VYN80_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1541909.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDFGKITIFNQTVEQNSENILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASL"
     gene            complement(474..644)
                     /locus_tag="VYN80_002087"
     CDS             complement(474..644)
                     /locus_tag="VYN80_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541910.1"
                     /translation="MKKENEYVILTTALLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(657..1102)
                     /locus_tag="VYN80_002088"
                     /pseudo
     CDS             complement(657..1102)
                     /locus_tag="VYN80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          354 a          217 c          119 g          417 t
ORIGIN      
        1 aacaatgatg cagctatact tagacgttgt ttcatgccca ttgaaaaatt tttcactttt
       61 ttctttttaa catcttttaa tcctaccatt gatagatatt cattaagtct ttcagtatct
      121 aaactaagcc ctttcatatt tgctattaac ttcaaatttt tataagcatc aagatgacca
      181 taaaatgaag gtcccaaaac taatcctatc ctagataata tattttcgga attctgttct
      241 acggtttgat taaaaatagt tattttccca aaatctggtt ttaaaatccc tagaatcatt
      301 ttgataattg tagtttttcc agccccattt ggtccaacaa aaccatatac ttttccctta
      361 tttaaataaa atgaagtgtt atgaataact tcaatatctt taaagctttt tgttagattt
      421 ataatttcaa ctatagccat ctctactcct tagtttacag caaaaaatta tgcttaattc
      481 ttattgtttt tgtaaacaat cagcgaaccc aatactattc cattcaacaa gcctaaagaa
      541 ataccatatt caactggaaa atctaaaaaa atagcaaaca ctattccaat catcacccct
      601 agtaaggctg ttgttaaaat tacatattca ttttctttct tcattttctt tacacctcat
      661 ttcctttttg tatataagct gtagtactct ccaccaagag tcattaagta cttatgattt
      721 cctgactcaa caatcttacc acctttcatt acaaaaataa tatccgcatc cttaatcgtt
      781 gacaatctat gagctataat tatttgagta caaccctgac tttgtatata ctttgttatt
      841 ctttcctcat taatagtgtc taatgcacta gttgcttcat ctaaaattac aatactagga
      901 ttatttatta atgcacgtgc cagtgctatc cgttgccttt gcccacctga aatatttgac
      961 cccatctctg agatgatagt attaaatttc atcggcatag ccatgatttc atcatagatt
     1021 tgaactgctt tacaaacttc ctctatcttt tgtgaagtaa ctagtgcttt aaagttatgt
     1081 tatcaagaat acttcggttc aataaaa
//
LOCUS       DAVLWA010000174         1104 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLWA010000174 DAVLWA010000000
VERSION     DAVLWA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.174"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            1..855
                     /locus_tag="VYN80_002089"
                     /pseudo
     CDS             1..855
                     /locus_tag="VYN80_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            848..>1104
                     /locus_tag="VYN80_002090"
     CDS             848..>1104
                     /locus_tag="VYN80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1541911.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIG"
BASE COUNT          416 a           97 c          220 g          371 t
ORIGIN      
        1 attttttata tatgttaaaa aaacttgaca agaatggatt tggaaaggtt aagacagtga
       61 taacatgcca tggatgggtt gaatataact taaaaaagaa attcttgact tattttgatt
      121 tttggacata ttctatggga gatgctttta tttgtgtatc tgaaactatg aaaaaaagat
      181 tggagagtat aataaaaaaa taagaagata gttgcaataa ataacggaat taatgtatca
      241 aatagcgatt tagatgtagt aggtgttcaa gactttaaaa aagaattttg tattccaaat
      301 aacaaaaaaa tcatttgtta tgttggaagg ttggatccag aaaaaaggcc ggatagattc
      361 cttgaatttg cagaaaaatt atttttagtc agagaagatg tgatttttat tatggctgga
      421 aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc atttgaaatg tagagataat
      481 ttcaggttac ttggtgaaat ctatccagct actatagttt accaaatatc ggatttattg
      541 tatattcctt ctgatacaga aggaatacct atgtgtgttt tagagtcaat gtcacaagga
      601 actccggttt tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca tagggttgat
      661 ggatttttat ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa ttttttactc
      721 aatgattctg agtatttgaa atatataggt gagaatagta aatcaaaaat aagaaaacat
      781 ttttctgtgc aaaaaatgtt tgtagaaacc atgagagtat atgatgaatt attagagaag
      841 agtagtcatg gataggaata tagatcaaga attagtaagt attataatac caacacataa
      901 taggtatgaa tctctgatta gagctgtgaa aagttgttta catcaaagtt ataaaaatat
      961 cgaagttata attattgatg ataattatag taatgtaaat ttaagaaata aaattatcca
     1021 tcaatttggc tataccaatc atagaattaa gttaatttta agtaatgaag atttaggtgc
     1081 aactaatgcc agaaacatag gtat
//
LOCUS       DAVLWA010000175         1100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLWA010000175 DAVLWA010000000
VERSION     DAVLWA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.175"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..968)
                     /locus_tag="VYN80_002091"
     CDS             complement(<1..968)
                     /locus_tag="VYN80_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1541912.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPFGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAIL"
     gene            complement(981..>1100)
                     /locus_tag="VYN80_002092"
     CDS             complement(981..>1100)
                     /locus_tag="VYN80_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1541913.1"
                     /translation="CYKSQILIIWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          380 a          197 c          123 g          400 t
ORIGIN      
        1 cttaaaatag ctgactcttt gtaattatct gaattatatt tcatccccaa tttatatatt
       61 cctactatct ttggctttcg ttccaatatt tgtttaacta tgaactgttt tctatttgtg
      121 tttgaaatat caatcgcttc tatcactggg gcatttattt ctataaattc tttttttaat
      181 tgtttagtat ctttgggaag acaatatcct ccaaatccaa aagaaagatt attataaaaa
      241 tttccaattc ttggatctaa acaaacacct tttattacaa cttcagcatt taaacttctc
      301 ctctcagcaa aagaatctag ttcattaaaa aaagcaacac ggagagctaa gaatgtgtta
      361 gaaaaaagct taattgcttc tgcttcagta ggagaaacta acataacatt tttaatattg
      421 gcagtactat gagtactaat cgaaaggaac aactctgcaa tttttcttcc ttcaactgtc
      481 tcatctccaa caactatgcg acttggatat aaattatcat atatagaaca accttctctc
      541 aaaaattcag ggacaaaaat gatatttttt gtatcaaaca gcctttttaa tttgtttgaa
      601 aagccgaacg gaactgttga ctttaaaata atctttccat taggttttac cctcagaatc
      661 ttcgataccg tttgttcgat ttcatatgta ttaaaactac caattttctc atcataatct
      721 gtcggaagcg caataatata ataatcaata ttatttttaa tttcagaaaa tgtatcaaaa
      781 aaagtaatat ttaagttatt ctcgcaaaaa aacttcataa gctcttcatt tttagatgga
      841 agaatgccct tttttaaatt atttattttt acagaatcta tatcatatgc aacaacttta
      901 tatttagatg caaatagtaa cgcgtaggcc agcccaacat gccccaaacc aattactgct
      961 atattcataa aactacttcc ttatttctta atccaaaatc taatagaata agctgcccca
     1021 ttccttaaat acaactcttt aatattgttt aaaagttttt caactgattt ccagattatc
     1081 aaaatctgag atttatagca
//
LOCUS       DAVLWA010000176         1087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLWA010000176 DAVLWA010000000
VERSION     DAVLWA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.176"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            340..>1087
                     /locus_tag="VYN80_002093"
     CDS             340..>1087
                     /locus_tag="VYN80_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1541914.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIET"
BASE COUNT          488 a          159 c          194 g          246 t
ORIGIN      
        1 aatcatctaa agatgaaata acataattgt cccttggtta caagggcgca attatacacc
       61 ctaaagggtg cttgcgtcct gcggagtcaa acccttgcag ggagcaacaa ccattcgctt
      121 tttaaaagtc aagggtaaat aaactcgcta aggctcgccc ttgactttta aaatttgaaa
      181 tgaaagtaac aattaagccg acatactgaa acaacaaaaa ttaattcagt agtcggcttt
      241 ttttattcta tcatttcaaa acgctcattc acaaagagga tataaaaaaa ctattaagcc
      301 tccacctaaa acaacaaaga aagaaaggaa gtgataaaaa tggcagacag ttttcatttt
      361 tcagtaaaca taatatcaag aggaaaaggc aaaagtgcag tagcaagtgc agcatatata
      421 agtggagaaa aaataaaaaa tgaatgggac ggagtaaccc atgactacac aagaaaagaa
      481 aaaatattag taaaaaatat aatattgcct gatcatatac caaaagaatt taatgatagg
      541 tctaccttat ggaataaagt agaaatggca gaaaaaaatt ctaatgcaca actagcaaga
      601 caattcataa taggactacc aaaagagtta tctttaagtg aaaataaaaa cctggttgaa
      661 agatatataa aagaaaattt aacatcacaa ggaatgatag tagattatgc aattcatgat
      721 gagagtcaag ataaaaatgg aaatatccat tgtcatataa tgaccataat gcgaccaata
      781 aacgaaaaag gagagttctt agccaagtca aaaaaagaat atatcctaga tgaaaaagga
      841 gaaaaagtat taaacaaaaa tggaaaacca aagacaagaa aagtagaact aacaacctgg
      901 aacgatacag gcaatgtaga acaatggaga gaaaattttt cagatctttg taacaaatat
      961 ttagaaagag caggagcaga aaaaagagta gaccatagga gttttaaaag acaaaattca
     1021 gattatctac cgacaatcca tttaggatca gcagcaagtg caatggaaag aaagggaata
     1081 gaaactg
//
LOCUS       DAVLWA010000177          984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLWA010000177 DAVLWA010000000
VERSION     DAVLWA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.177"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(119..832)
                     /locus_tag="VYN80_002094"
     CDS             complement(119..832)
                     /locus_tag="VYN80_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1541915.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
BASE COUNT          310 a          192 c          179 g          303 t
ORIGIN      
        1 ttgacaagtg tcagatgtaa attcataatt aacagcttca ttaaaatcaa cttcagacat
       61 ttcaatgtac tcttcaatag taatattagc tttttttgta gtcattagct aatctccttt
      121 aaaatgtgat cttttcaaga atatttttat ctaaacaatc cagcaagtct tggtaagaat
      181 agacttcgta agtcggctgg gcttgtgtgt gattttcgag gtgatgagga ttataccaga
      241 tagtgtcaat ccccgcatta ttgccacctt gaatgtcggc ggttagagaa tctccaatca
      301 tcagcgtctt ttctttacta aatccagcaa tttgctggcc aatcttttca taaaagagag
      361 catccggctt ttgagtttgc aactgttctg agataaagac ttgattgaaa taaggtgcta
      421 gaccagattg agccaaacgt cctgtctgaa tggcagtaat gccatttgtc gcagcataca
      481 agtcataatc acgctcaatg aggctgtcca agagatcatg agcgcccgat agtgtttgtc
      541 cctgctgggc gaggtaaaat tggtaacgct gggcaagaaa actaccgtct ttttcctgtc
      601 caaaatgaga aaataaacga gaaaagcgcg tgttaaccag ctcttgttta ctgattttct
      661 tcagctccaa gtctttccag agagccttgt tcataggaac gtaataatct ttataagcct
      721 gaatatccgc aactccttct tcttttagaa gttgggtcaa agccacatcc tcagcagcat
      781 caaaatcaag aagagtgtgg tcgaggtcga agagtagaaa tttgtagaac aatttgaggt
      841 tttcctttct gaaaattcat taagaacatt atatcataaa gcacctcata caattaacta
      901 atttaatcac ttaaaaaaaa ttcgaacact ttctatacaa ctgacagctc aaatctttca
      961 gaatagaaca atactaacta tcga
//
LOCUS       DAVLWA010000178          983 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLWA010000178 DAVLWA010000000
VERSION     DAVLWA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.178"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..559
                     /locus_tag="VYN80_002095"
     CDS             <1..559
                     /locus_tag="VYN80_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541916.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(513..>983)
                     /locus_tag="VYN80_002096"
     CDS             complement(513..>983)
                     /locus_tag="VYN80_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1541917.1"
                     /translation="DLSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLK
                     HNQLITPVATSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPL
                     FYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
BASE COUNT          354 a          156 c          146 g          327 t
ORIGIN      
        1 gaacaaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat tttcaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgctcagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgctac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atc
//
LOCUS       DAVLWA010000179          972 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLWA010000179 DAVLWA010000000
VERSION     DAVLWA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.179"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            99..864
                     /locus_tag="VYN80_002097"
                     /pseudo
     CDS             99..864
                     /locus_tag="VYN80_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          361 a          108 c          177 g          326 t
ORIGIN      
        1 taatggagga ataaaaaatg taagttataa atgtaagtaa gcattatggt cattcaatca
       61 ttctcaaaga tataaatttt gcacttaaca agggtgaaat tgttggtcta gtagggagaa
      121 atggagttgg taagagtacg ttgatgaaaa ttcttgttca aaataatcaa ccgacttcag
      181 gtaatattat aaacagtgat aatgttgggt atttaatcga agaaccaaaa ttatttttat
      241 ctaaaacagg tttagagaat ttaaaatatt tgtcaaattt atatggtgtt gactacaatc
      301 aagaaagatt tagaagtttg atccaagagt tagatttgac tcagtctatt aataaaaaag
      361 taaagaccta ttctttgggt acaaaacaaa aattagcttt gcttctaact ctcgttacgg
      421 aacctgatat attgatttta gatgaaccga ctaatggttt agatattgaa tcatcacaaa
      481 tagttttagc ggttctaaaa aaaattagct ttaaatgaaa acgtgggaat tttaatatcg
      541 agtcataaat tagaagacat tgaagaaatt tgtgagaggg ttcttttctt ggagagcggg
      601 cttttgacat ttcaaaaagt aggaaaagat agtcataatt tcttgtttga gatagctttt
      661 tcatcagcta cagatagaga cattttcatt accaaacaag aatttgggga tattgttcag
      721 gaagagggat tgagaattac tatgtctggg aatattcaaa atagtgagct ttttaaattt
      781 tttaacgaaa actctattaa agtagttgat tttgaaacta aaaaagagac gcttaaagat
      841 atttacctaa atcgttcaaa ataaaggaag gttataatca tgaaattaaa taaacagaag
      901 aatcggatga tttacgtctt gtctaatttt ctatatgcca tctcagtttc cattatttat
      961 gctttgaatg gc
//
LOCUS       DAVLWA010000180          967 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLWA010000180 DAVLWA010000000
VERSION     DAVLWA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.180"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            22..>967
                     /locus_tag="VYN80_002098"
     CDS             22..>967
                     /locus_tag="VYN80_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1541918.1"
                     /translation="MEDLISIVVPVYNVEKYLKKSIESILNQTYQNIEILLVDDGSTD
                     SSGKICESFSKVDPRIRVFHKENGGLSDARNFGIEQMKGQYVAFIDSDDYISKDYVWK
                     LYSSIKNNDSEVSICSFLLVDEKGEKIKDELLDSGKICLTGQQILEKVLTADGYRYVV
                     AWNKLYRSTLFEKLKFKKGMLYEDEFLNYPLFWDCKRVSIVEEPLYLYVQRKGSIVQS
                     NMTLEKIKMKDEMHTSRIEFYSEKGHSFLHEKACQQYCNWIVTATTNHSKILNPNFSK
                     YLQRQFRKFAKYTRNNDIRLIVQNILGFIDIRLAAYVKS"
BASE COUNT          358 a          108 c          192 g          309 t
ORIGIN      
        1 aaaatgaagg ataaataagt tatggaagat ttgataagca ttgttgttcc agtctataac
       61 gtggaaaaat atttaaaaaa atcaatagaa agtattttga atcagactta tcaaaatatc
      121 gagattttat tggttgatga cggaagcaca gatagtagtg ggaaaatttg tgaatcattt
      181 agtaaagttg atcctaggat aagagtattt cataaagaaa atggtggttt atcagatgct
      241 cggaattttg gaattgagca aatgaaaggt caatatgtag cgtttattga tagcgatgac
      301 tacatatcta aggattatgt ctggaagttg tattcttcta taaaaaataa tgattccgag
      361 gtgtcgattt gttctttttt attagtcgat gaaaaagggg aaaaaataaa agatgagcta
      421 ttagattcgg gaaaaatatg cttgactggt caacaaatat tagaaaaagt attaacagcc
      481 gacggctatc gctatgttgt tgcttggaat aagctttatc ggtcaacttt atttgaaaaa
      541 ttaaaattta aaaaaggaat gttgtatgag gatgaatttc ttaactatcc tctattttgg
      601 gactgtaaaa gggtatcaat tgtagaggag ccgttatatt tatacgttca acgaaaagga
      661 agcattgtac aaagtaatat gactttagaa aaaataaaga tgaaggatga gatgcatact
      721 tcacgcattg agttttattc agaaaagggg cattcttttt tgcacgaaaa agcgtgtcaa
      781 cagtactgca attggattgt tacagcgact accaatcata gtaagatttt aaatcctaat
      841 ttttcaaagt atttacaacg acagtttaga aagttcgcta aatatacacg aaacaatgat
      901 attagactaa ttgtgcagaa cattctagga tttatagata ttcgtttagc agcttatgta
      961 aaatcaa
//
LOCUS       DAVLWA010000181          943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLWA010000181 DAVLWA010000000
VERSION     DAVLWA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.181"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..777)
                     /locus_tag="VYN80_002099"
     CDS             complement(<1..777)
                     /locus_tag="VYN80_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1541919.1"
                     /translation="MIEVSIIIPIYNAEKTIKNCVDSALKQNLESLEVILVNDGSNDS
                     TAEILNQYDRNSRVKIFHQLNKGVSTARNKGLSHASGEYVFFLDSDDILDDGMLSKMY
                     QFVKNNKVDLLSCWHKEPATTQYGGNENKSTSFIARTREEIGAHFVDIFPRSACAKLF
                     FRKIIEENNITFSTEMSLGEDMSFVYQYLMVSKSIGVIDGVYYNIQNVNPQSLSKRYV
                     QNIENSLIIQNQLWNQLLEVYPKIEENYYKQHMDFRFYLAN"
BASE COUNT          329 a          174 c          117 g          323 t
ORIGIN      
        1 gttagcaagg taaaatctaa aatccatatg ttgcttatag tagttctcct caattttagg
       61 ataaacttcc aacagttgat tccataactg gttctgaatt attagggaat tttcaatatt
      121 ctgcacatag cgcttagata acgactgtgg atttacattt tgaatattat aatacacccc
      181 gtcaataact ccaatacttt tagaaaccat taagtattga tacacaaatg acatatcttc
      241 acctagtgac atctcagtcg aaaaagtaat attattttct tcaattattt tgcgaaaaaa
      301 caatttggca caagcacttc tcggaaagat atcaacaaaa tgagcaccta tttcttctct
      361 tgtacgtgca ataaatgaag ttgacttatt ctcatttcca ccgtattgag ttgtagcagg
      421 ttctttatgc caacaagata agagatcaac tttattattt tttacaaatt ggtacatttt
      481 agaaagcata ccatcatcta aaatatcatc tgaatctaga aaaaatacat actctccact
      541 agcatgagat aaccccttat ttctagcagt agacacccct ttattaagct gatgaaaaat
      601 cttaaccctt gaattcctgt cgtactggtt caaaatttct gctgtagaat cgttagaccc
      661 atcattcacc aaaatcactt caagagattc taaattttgc tttaaggcac tatcaacaca
      721 atttttaata gttttttcag cattgtatat tggaattatg attgatactt ctatcatcca
      781 actatttcct cttttctatt gtttatgaaa atacttattg ttgaaaaaaa taaaattaaa
      841 caccaataat gtggatctaa ttcttcaaaa agccaaaaca ccaataaagc taaaatatag
      901 cctatactga ttaactttcc agatggggca ctttttaaaa att
//
LOCUS       DAVLWA010000182          921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLWA010000182 DAVLWA010000000
VERSION     DAVLWA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.182"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            68..250
                     /locus_tag="VYN80_002100"
     CDS             68..250
                     /locus_tag="VYN80_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1541920.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            260..625
                     /locus_tag="VYN80_002101"
     CDS             260..625
                     /locus_tag="VYN80_002101"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1541921.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            622..>921
                     /locus_tag="VYN80_002102"
     CDS             622..>921
                     /locus_tag="VYN80_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1541922.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGA"
BASE COUNT          375 a          115 c          153 g          278 t
ORIGIN      
        1 aatcgaaaaa ttagctaaga aatattctga taacttaaac atcaaagttc aagagagagt
       61 tcgtgaaatg gcaaatgata ataagagcca ttatttgata tacagagttt taggtatttc
      121 atttgaagaa ggagaaaata tcgatttgta tcaaaataaa ggtcgttttt tatacaaata
      181 tgctggttca tttttagaag aagctgcagt actatgcttt aacgaaaaat ttggtacaga
      241 aaatacttaa aaagttaaca ttcctaattc tgaaagtaca aaacctaaga cttttgaaat
      301 tgattgttta gtcggagaaa aacacgcata cgaaataaaa tggtgggatg caactacaga
      361 tggagaccat ataactaaag aacacactag aataaaagtt attcataaca aaggatatat
      421 accaattcgg ttaatgttct actatccaaa tagaactcaa gctataaaaa ttcagcaaac
      481 tttagaaaca ttgtataacg gtattggagg gaaatattat tatggagatt ctgcctggga
      541 acatttaaga gcagtgaccg gtattgattt acttagtatt ctaacagata ttgcaaataa
      601 aaaaacaggg gtaaaatcaa aatgacagta ttaaaaggag ataacttaga aatattaaaa
      661 actattgaat cctcaagtat tgatttaatc tatatggacc ctcctttctt tacacagaaa
      721 acccaaaaat tatctaataa caaaaatatt atgtattcat tcgaagatac gtggacttcg
      781 attgaggatt acaaagaatt tttgtctgta agattagaag aatgcaaaag agtgctaaaa
      841 aatagtggca gtattttcgt tcattgtgat aaaattgcaa atcatcatat tagattaatt
      901 ttagataata tctttggagc a
//
LOCUS       DAVLWA010000183          875 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLWA010000183 DAVLWA010000000
VERSION     DAVLWA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.183"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..850)
                     /locus_tag="VYN80_002103"
     CDS             complement(<1..850)
                     /locus_tag="VYN80_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1541923.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLI"
BASE COUNT          327 a          150 c          129 g          269 t
ORIGIN      
        1 ttattagtaa gtatattaag tatggtgaac ctaacaagcc agttacaaca cctactggat
       61 atctagctgg taaaatattt tgagagaata tgtctgataa caaaactagt aaaattccaa
      121 ccaatccagc taatattggg cttcttttct tgccaatatt taaggctatg ggaccagcta
      181 aaaaagatat acaagctatt ggtcctgtaa ttgaagtaga aaaagcagtt aaagatacag
      241 cgcaaaaaat taaaacaagc cttgaaagct cgggatttgc tccaagtccg attgctattt
      301 cttcaccaag ttcaataatt tctagtcttt tattaaaaaa taaaactaat atagtagcaa
      361 taatacttac tattagaaca agaggtatgt catctaactt tgtaaaagat aaagagccac
      421 tgagccatct cataacttct tgtaattcat atcttgctac tttcaacaat aaaaatgagg
      481 tgcctgctct tgtgacagct tgaaaaccaa tacctaatat tatcagtctt gctgctgaaa
      541 aaccatcttt tttagctagt aaaaataata ttaaagatga tgttagtcca caagttattg
      601 aaataattcc agtagttaaa ctatttgttt ttaataccaa tatgcaaaag accgctgcaa
      661 tagatgaaga acttgtgaca ccgattatat caggacttgc aagaggattt cttaacatag
      721 tttgaaagat aaatcctgcc aatccaaaag accagccagc tataattcct gctaataatt
      781 ttggtaatct aatttccata atcgaaaaac tagctccagg aacagtttca ctatttaaga
      841 ctttaatcaa agttgaaaaa gaataacttt catct
//
LOCUS       DAVLWA010000184          834 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLWA010000184 DAVLWA010000000
VERSION     DAVLWA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.184"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..816)
                     /locus_tag="VYN80_002104"
     CDS             complement(<1..816)
                     /locus_tag="VYN80_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1541924.1"
                     /translation="MPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLS
                     VVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMAL
                     SSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNAL
                     AMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDI
                     RTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRG"
BASE COUNT          297 a          164 c          127 g          246 t
ORIGIN      
        1 ccctctattt ttcatagtta cataaataag tactggaccc ccgattattg cagtaattat
       61 ccctacttca atttcacctg gtttacctaa catacggccg attatatcac atataagcaa
      121 gagctctgca cctataaaag atgaagaaat ggtcattgtg cgtatatctt tgcttataaa
      181 taagccacaa aagtgaggaa ctataagacc tacgaagcca ataggtccac caattgcagt
      241 aatacttgaa cataaaagca cacttgcaat tattgcaagt gatcttatcc tattaacatt
      301 aactccaaga ccaacagcca tttcatcacc catagctaaa gcgtttaaat ctgatgaaat
      361 aaatatagct atcaagtgac ctaaaattat aaaaggtagt agtgtagata tagaagataa
      421 tgtagctgct ccaaggctac ctatttgcca aaatctaaat ttgtctaaga cgttattatt
      481 cggtaaaatt aaaaaactta caaaactgct taaagccata ctaacacaag ttcctgataa
      541 ggcaagtttt ataggggtaa ggcctgcttt tccgcttaca gcaatcgcgt atacaaaaat
      601 tgcacttact aagccaccaa tgattgcaaa acttatatgg cttatgcttg atgaaattcc
      661 taaaaaagaa agaccaatta ctacactaag acttgctcct gtgtttatac cgagtatacc
      721 tggatcagct attgggtttc tagtaactga ttgcatcaat acaccgctta tggcaagact
      781 agaacctgct aaaattgcaa aaatagttct aggtattctt ttatatataa ttga
//
LOCUS       DAVLWA010000185          827 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLWA010000185 DAVLWA010000000
VERSION     DAVLWA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.185"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            67..>827
                     /locus_tag="VYN80_002105"
     CDS             67..>827
                     /locus_tag="VYN80_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
                     /protein_id="HEV1541925.1"
                     /translation="MLADVLALVDAEAEALVDAEAEALVDAEAEALVDADADALVDAD
                     SEALVNAEAEALVDAEAEALVDAEADALVDADSDALVLAEADALVDAETDALVLAEAD
                     ALVDADSDADVLAEAEALVDAEAEALVDAEADALVDAKADALVDADSDAEVLAEAEAL
                     VDADSDAEVLAETDALVLAEAEAEALVDADSDAEVLVDAEAEADVDADSDALVLAEAE
                     ALVEAEAEALVLAEADALVDAEADALVDAEAEALV"
BASE COUNT          154 a          189 c          276 g          208 t
ORIGIN      
        1 gctgaggccg aggcgctggt tgacgctgaa gctgaagcac ttgtgcttgc tgaggctgat
       61 gcacttgtgc ttgctgatgt acttgcgctg gttgacgctg aagctgaggc actcgttgat
      121 gctgaagctg aggcgctggt tgacgctgaa gctgaggcgc ttgtcgatgc tgatgctgat
      181 gcgcttgttg atgcagattc agaggcgctt gtcaatgctg aagctgaggc actcgttgat
      241 gctgaagctg aggcgcttgt cgacgctgaa gccgacgcac tcgttgatgc tgactcagac
      301 gcacttgtac ttgctgaagc cgacgcactc gttgatgctg agactgatgc gctggtactt
      361 gctgaggctg acgcactggt tgacgctgat tcagatgctg atgtgcttgc tgaggctgag
      421 gcactcgttg atgctgaagc tgaggcgctt gtcgacgcgg aagctgacgc actcgttgac
      481 gctaaagccg acgcacttgt tgatgccgat tcagacgctg aggtgcttgc tgaagctgag
      541 gcactcgttg atgccgattc agacgctgag gtacttgctg agactgatgc gcttgtgctt
      601 gctgaggctg aagctgaggc actggttgat gctgattcag acgctgaggt acttgttgac
      661 gctgaggctg aggcagacgt tgatgctgac tcagacgcac ttgtacttgc tgaagctgag
      721 gcactcgttg aggctgaagc tgaggcgctt gtacttgctg aagccgacgc actggttgac
      781 gctgaagccg acgcacttgt cgatgctgag gccgaagcac tggttga
//
LOCUS       DAVLWA010000186          805 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLWA010000186 DAVLWA010000000
VERSION     DAVLWA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.186"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            134..403
                     /gene="blpJ"
                     /locus_tag="VYN80_002106"
     CDS             134..403
                     /gene="blpJ"
                     /locus_tag="VYN80_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1541926.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            472..720
                     /gene="blpK"
                     /locus_tag="VYN80_002107"
     CDS             472..720
                     /gene="blpK"
                     /locus_tag="VYN80_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV1541927.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          250 a           98 c          199 g          258 t
ORIGIN      
        1 actgctacct ttaagaagat agtagacgta tatacttttt taagaaaatc aaaaagatac
       61 taaaaaaaaa tctatttgtt tattgaattt aagactttgg taacaaattg aaaataaaaa
      121 ggaggtattc atcatgaata caaaaatgtt gtcacagtta gaggttatgg atactgaaat
      181 gcttgcgaaa gttgaagggg ggtatagctc cactgattgt caaaatgcac tgattacagg
      241 agtcactacg ggaattataa ctggtggaac tggagcaggt ttggccactt taggagtagc
      301 tggacttgct ggtgcatttg ttggagcaca cattggtgca attgggggtg gattaacatg
      361 cttaggaggt atggttggtg ataaattggg actaagttgg taagacttat agtaaaatta
      421 tgaggattct attctgattt attttagttt tttaaaaaag gagtatttgt catggataca
      481 aaaatgatgt cacaattttc tgttatggat actgaaatgc ttgcttgcgt tgaaggtggc
      541 ggatgcaatt ggggagattt tgccaaagca ggtgttggag gagcagctgt agttgctgct
      601 ttaggttgtg cagcgggtgg agtgaaatat gggaaaattc taggaccatg gggcgctgca
      661 ataggaggaa ttggaggagc agtggtttgt ggatatttag cctataccgc tacatcataa
      721 aaatgaaagc taaattttat gaaggctata ttctttatta ttctcttcgc ttttcaaacc
      781 tatcttattt atctatccat aagta
//
LOCUS       DAVLWA010000187          759 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLWA010000187 DAVLWA010000000
VERSION     DAVLWA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.187"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..577)
                     /locus_tag="VYN80_002108"
     CDS             complement(<1..577)
                     /locus_tag="VYN80_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1541928.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGE"
BASE COUNT          225 a          143 c          123 g          268 t
ORIGIN      
        1 cctctccctc ttcctcgcta aaagtagcta gagctccgtt ttgaatacca ttacagctaa
       61 cgaaagcttt agaaaattgg agattagaga gattttgtag ggtcaatgta ccaacaaaag
      121 cacctgtaat atcgcgataa tttccaccta ttaaaatcaa atctgttaat tttcgttcgc
      181 ttaaaatcag aaaaacaggt agactgttgg ttacgacgcg gatattgtca ataggcaact
      241 cacgcgcaaa aaactctaat gttgttcctg gtccaatgaa aatagtttct ctttcttcta
      301 ctagactgcc tgcaaaatgg gctatttctt gtttttctgc cgtttggagg gcttgttttt
      361 caatatttga tcgctcatta gtcaaaaggg agttggttcg aagtttttca gctccaccat
      421 gcacacgaat cagcaaatct ttatcagcta attcctgtaa atagcgcctt gcagtcatat
      481 ctgaaacggc tatttcgtcc ataatctgtt taactgttat ggttccttta ctatttacta
      541 tctctaaaat tttggttaat ttttcttgtt tgagcatatt atcacctcgt ttcctactac
      601 tatcttacca taaacaaact catcattcaa atacaaaaac aacaaaatga aacaaaaaca
      661 aaaatatcga agtttgtttt caaaactttc gatatttttg ttgggttatt attttgttgt
      721 ttctagttta ctttttgatg gttaagagtg gtggagaat
//
LOCUS       DAVLWA010000188          709 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLWA010000188 DAVLWA010000000
VERSION     DAVLWA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.188"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..674
                     /locus_tag="VYN80_002109"
     CDS             <1..674
                     /locus_tag="VYN80_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1541929.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
BASE COUNT          327 a           91 c          135 g          156 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttt
//
LOCUS       DAVLWA010000189          642 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLWA010000189 DAVLWA010000000
VERSION     DAVLWA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.189"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..580)
                     /locus_tag="VYN80_002110"
     CDS             complement(<1..580)
                     /locus_tag="VYN80_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1541930.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMY"
BASE COUNT          218 a          128 c           81 g          215 t
ORIGIN      
        1 gatacatttt taattcttta gtttttccca tataactaac catactacta tcacttacat
       61 atgggaagtc ctcataatat attactttat aacgcataaa ttcaagcgcc cttccaatac
      121 tattcacaaa aacatgatca acatggtcac caagtgaaag cggacaatat acgacacatt
      181 tgtcgtctaa atgcattaac agctctttta tgatatcatt ctttaatgtg tcctcatttt
      241 ttaattcact atagatatga cggtatagaa aattgccatt tctatctttc ctatagagac
      301 attcatagta cgataagtgt ctaaaatcac actgtagacg ttcacaagct aacctgtctt
      361 ctttcttcct ttcttcaatc ggatatttcc caaggttaca caacttatga aattgcttag
      421 cagagggctg tagctgttgg ctcaaagggt aaccagaaaa tatagtaata acaagtacaa
      481 tttctccttc tgaagttaat tttgaaatat aatcaccaca ggaaaaaatt gcgtcatcta
      541 aatgtggaga taaaaagata tacttagtat tgttactcat aaccattccc tctacaattt
      601 atctaaaaac tcactaagtg tctgattaaa ttccacatca tc
//
LOCUS       DAVLWA010000190          627 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLWA010000190 DAVLWA010000000
VERSION     DAVLWA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.190"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            270..569
                     /locus_tag="VYN80_002111"
     CDS             270..569
                     /locus_tag="VYN80_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1541931.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          236 a          122 c          106 g          163 t
ORIGIN      
        1 caaacaaagc atacgaacct ttagtaaaat catttccatg aaactagaat agagccctct
       61 tagcaaaaat cattatttta atttatttct aatcactcct tgacataaat aactctcacc
      121 aataaaagac tatgtcttaa aaaaatggta taataaaatc aatacttggg cttgatggct
      181 atgctactaa taacaattag gagagaaaat caggcacttg ttaacaacaa ggattatccc
      241 cttgagatga aaggaacttt agaaatctta tgatgaacat gcaaaacatg atgcgtcaag
      301 cacaaaaact tcaaaaacaa atggaacaaa gccaagctga acttgctgct atgcaatttg
      361 ttggcaaatc tgctcaagat cttgtccaag cgaccttaac tggcgataag aaagttgtca
      421 gcattgattt caatccagct gtcgttgacc cagaggacct tgagactctt tctgatatga
      481 ccgttcaagc catcaactct gctcttgaac aaatcgatga aactaccaag aaaaaactgg
      541 gtgctttcgc tgggaaatta cctttctaaa aacaaggagc tagaacaatg cttgtcgata
      601 acaaaggcta agaaaggtgc aaaaatg
//
LOCUS       DAVLWA010000191          598 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLWA010000191 DAVLWA010000000
VERSION     DAVLWA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.191"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..>598
                     /locus_tag="VYN80_002112"
     CDS             <1..>598
                     /locus_tag="VYN80_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541932.1"
                     /translation="EYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLL
                     RCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFD
                     EEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPSSGMDYSN
                     MIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          233 a           69 c          112 g          184 t
ORIGIN      
        1 gagagtatca gataaaaaat cttagctaca aatttactga tgatgagtgt ttaagcttaa
       61 aagatatttc attcaagctt gggaaaattt atggcataat aggatccaac ggacgaggaa
      121 aatcaacgct tttaagatgt ttaataggtc ttgagaaaaa atcaaaagaa gaaatttatt
      181 ttaagggaga gaagctatct aaaaaagaaa gactcaaaaa ctcttcactt gttatgcaag
      241 atgtaaatca tcaattattc acagatgaag tattcaacga gcttagatta ggagtaaaga
      301 attttgatga agaaaaggcg aaaatcattt taaaagattt aggcctggac gaatttattg
      361 aaaggcatcc gatgagttta tcaggagggc aaaagcaaag gcttgcaata gcatctgtta
      421 tgtgcaagaa ttctccattt atcttttttg acgaaccttc aagtggtatg gattattcca
      481 atatgataaa aatatctgaa ctgattaata agtataaaac catggataaa ataattttta
      541 ttgtttccca tgatatagaa tttttaaatg aagtggcaga tgaaattttt gaattgta
//
LOCUS       DAVLWA010000192          587 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLWA010000192 DAVLWA010000000
VERSION     DAVLWA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.192"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(<1..339)
                     /gene="tnpB"
                     /locus_tag="VYN80_002113"
     CDS             complement(<1..339)
                     /gene="tnpB"
                     /locus_tag="VYN80_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1541933.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESR"
     gene            complement(320..505)
                     /locus_tag="VYN80_002114"
     CDS             complement(320..505)
                     /locus_tag="VYN80_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1541934.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
BASE COUNT          188 a          119 c          111 g          169 t
ORIGIN      
        1 acgactttct gataaattta ttttgggagt gatagaaaag cccttcataa gccaatctac
       61 ttgttcaggt gtgagagctt tgacatcctt ttctgtactg ggccaagtca gtctgccgtt
      121 ctcaaagcgt ttatatagta gccaaaatcc ttgaccatcc cagtaaaggg ctttaaagcg
      181 gtctttacgt ccaccacaaa agagaaaaac ttgaccggag aaaggatcca attcaaagtg
      241 ggttttaacc agataagcca gtgaatcgat tccttgtctc atatcagtct tcccacacac
      301 gagatagacc tgccctaggc tagatagatg aattgtcata gagcaacacc ttatccaaaa
      361 gctgttctac catttcgaga ttgagagatt gaaaaaaact tacttcaagc ttgccaatac
      421 gaattttaag cagaatatca tttctctttt tagaatcaaa aggacgagat tgtggaatct
      481 ctacaggaac aataggttgt ggcattaaaa tatcctccaa tagttttact ttactaatag
      541 tatacaggag gaactccatg aatgatagat accttgttat gacgcgc
//
LOCUS       DAVLWA010000193          469 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLWA010000193 DAVLWA010000000
VERSION     DAVLWA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.193"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            7..>469
                     /locus_tag="VYN80_002115"
     CDS             7..>469
                     /locus_tag="VYN80_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1541935.1"
                     /translation="MSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFV
                     GNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVRE
                     FLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYY"
BASE COUNT          173 a           48 c           95 g          153 t
ORIGIN      
        1 gaatatatgt cagatcgaat tttaaagttg atggcttgtt ttaaaaagag tagaatgaag
       61 aatttagctt tagtttatag ttatggaata ataatttatc caaatggtac acgagaagag
      121 gagaagaccg attttgttgg aaatcccttg tttgttcaaa tggttcacaa tatagcaggt
      181 acgtcatttt ggttgtgtaa aaaagaggtg ctagaattaa ttaatggttt tgagaaaata
      241 gattcacatc aggacggtgt tgttttatta aaactacttg ctcaaggata ccaaattgat
      301 atagtgcgag aattcttggt gaattactac gctcacagta aagaaaacgg tatcactgga
      361 gtgacacaaa aaacaattaa tgcagatgaa gaatattata attactgtag gaaatatttt
      421 aatttattga gtttcaacga gagaatattg gttacaaaga aatattatt
//
LOCUS       DAVLWA010000194          438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLWA010000194 DAVLWA010000000
VERSION     DAVLWA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.194"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            complement(1..357)
                     /locus_tag="VYN80_002116"
                     /pseudo
     CDS             complement(1..357)
                     /locus_tag="VYN80_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          137 a           72 c           95 g          134 t
ORIGIN      
        1 aaaggtttta aagacagtct gaaaaagagg atgaacctgc ttaagattgt cctcaataag
       61 tccgaaaaat ttctctggtt ccttattata gagctgatag tggtgtttca agtcttctga
      121 atagctcaaa agcttgttta aaatctcttt attggttaag tacatacgaa aagtaggacg
      181 ataaaatcgc ttattactca gtttacggct atcctgttga atgagtttcc agtagcgctt
      241 gatagccttg tattcatgag actttcgatg aaactgattc atgatttgga catgcacacg
      301 actcatagca cggctaagat gttgtacaat atgaaagcga tcaagaacga ttttagcatt
      361 cgggagtgaa acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagt
      421 tgtctagcta agtcatag
//
LOCUS       DAVLWA010000195          433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLWA010000195 DAVLWA010000000
VERSION     DAVLWA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.195"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            36..>433
                     /locus_tag="VYN80_002117"
     CDS             36..>433
                     /locus_tag="VYN80_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV1541936.1"
                     /translation="MSLSLSMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINV
                     LSQKIHKYNSDVQVVPISIKVSSVEELENIVAEYGSIDFIVKAIDTPIDIIKIVNQFA
                     VSHKISYISGGFNGCYLIIDNIYIPTIGSC"
BASE COUNT          145 a           57 c           80 g          151 t
ORIGIN      
        1 tattagtatt aggagccgga gccttaggat gttatatatc tctaagtcta agtatgtatg
       61 gagtgaggaa acttattgtc gctgattacg atataataga accatcaaat ttaaataggc
      121 aaattcttta tacagagtcg gatgttggta aggagaagat taatgttctt tctcaaaaaa
      181 tacacaagta taattcagat gttcaggtag tacctatttc tattaaagtt tcttcagtag
      241 aagaattaga aaacattgtt gcggaatatg ggagtataga ttttatcgtt aaagcaattg
      301 atacgcccat tgatattata aaaattgtca atcaatttgc tgtatcgcat aagatatcct
      361 acatatcagg agggtttaat ggatgctatc ttattattga taatatatat atccctacca
      421 tcggttcttg ctt
//
LOCUS       DAVLWA010000196          427 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00046183
            SAMN33607121-rid17946233.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLWA010000196 DAVLWA010000000
VERSION     DAVLWA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607121
            Sequence Read Archive: SRR23725577
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721454.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721454.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:40:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,118
            CDSs (total)                      :: 2,072
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 63 of 123
            Pseudo Genes (incomplete)         :: 53 of 123
            Pseudo Genes (internal stop)      :: 34 of 123
            Pseudo Genes (multiple problems)  :: 23 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607121-rid17946233.denovo.196"
                     /strain="AUSMDU00046183"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2020"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST199"
     gene            <1..385
                     /locus_tag="VYN80_002118"
     CDS             <1..385
                     /locus_tag="VYN80_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1541937.1"
                     /translation="LFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYV
                     GDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVI
                     IVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          152 a           45 c           92 g          138 t
ORIGIN      
        1 tttgtttgaa ggtacgatta gagataatat tttattggaa gaaaattata ctgatgaaga
       61 aatactacag tctttagagc aagttggttt gagtgtaaaa gattttccta ataacatttt
      121 agattattat gttggtgatg atgggagatt actgtcagga gggcagaaac aaaaaattac
      181 tttagctaga gggctaatta gaaataagaa aatagtatta attgacgagg gaacttctgc
      241 tatcgatagg agaacttcgt tagcgattga acgtaagata ttagatagag aggatttgac
      301 tgtcattatt gttacccatg ctccgcatcc ggaacttaaa caatatttta ctaagatata
      361 tcaatttcca aaggatttta tttaagaata taggggaggg aaacatgaga ttctattgtg
      421 aagctta
//
